LOCUS       DAVKJU010000001        81533 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVKJU010000001 DAVKJU010000000
VERSION     DAVKJU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 81533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81533
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.001"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..439)
                     /locus_tag="VW191_000001"
     CDS             complement(<1..439)
                     /locus_tag="VW191_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9622857.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSVTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKA"
     gene            complement(884..1282)
                     /locus_tag="VW191_000002"
     CDS             complement(884..1282)
                     /locus_tag="VW191_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9622858.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(1610..2092)
                     /gene="greA"
                     /locus_tag="VW191_000003"
     CDS             complement(1610..2092)
                     /gene="greA"
                     /locus_tag="VW191_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU9622859.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(2163..3818)
                     /gene="mltG"
                     /locus_tag="VW191_000004"
     CDS             complement(2163..3818)
                     /gene="mltG"
                     /locus_tag="VW191_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU9622860.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(3899..4393)
                     /locus_tag="VW191_000005"
     CDS             complement(3899..4393)
                     /locus_tag="VW191_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9622861.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(4390..4854)
                     /locus_tag="VW191_000006"
     CDS             complement(4390..4854)
                     /locus_tag="VW191_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9622862.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEETLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(4864..6198)
                     /locus_tag="VW191_000007"
     CDS             complement(4864..6198)
                     /locus_tag="VW191_000007"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU9622863.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(6209..6826)
                     /locus_tag="VW191_000008"
     CDS             complement(6209..6826)
                     /locus_tag="VW191_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9622864.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(6874..9972)
                     /locus_tag="VW191_000009"
     CDS             complement(6874..9972)
                     /locus_tag="VW191_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9622865.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(10065..11231)
                     /locus_tag="VW191_000010"
     CDS             complement(10065..11231)
                     /locus_tag="VW191_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9622866.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNKSKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(11239..12333)
                     /locus_tag="VW191_000011"
     CDS             complement(11239..12333)
                     /locus_tag="VW191_000011"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU9622867.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKLFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(12490..14172)
                     /locus_tag="VW191_000012"
                     /pseudo
     CDS             complement(12490..14172)
                     /locus_tag="VW191_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14344..16302)
                     /locus_tag="VW191_000013"
     CDS             complement(14344..16302)
                     /locus_tag="VW191_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9622868.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGIESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(16506..18128)
                     /locus_tag="VW191_000014"
     CDS             complement(16506..18128)
                     /locus_tag="VW191_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9622869.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     FQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            18234..19679
                     /locus_tag="VW191_000015"
     CDS             18234..19679
                     /locus_tag="VW191_000015"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEU9622870.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(19722..19919)
                     /locus_tag="VW191_000016"
     CDS             complement(19722..19919)
                     /locus_tag="VW191_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9622871.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKVKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(20020..20628)
                     /locus_tag="VW191_000017"
     CDS             complement(20020..20628)
                     /locus_tag="VW191_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEU9622872.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(20653..21165)
                     /locus_tag="VW191_000018"
     CDS             complement(20653..21165)
                     /locus_tag="VW191_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU9622873.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAAFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(21251..22186)
                     /locus_tag="VW191_000019"
     CDS             complement(21251..22186)
                     /locus_tag="VW191_000019"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEU9622874.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEYGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(22257..22556)
                     /locus_tag="VW191_000020"
     CDS             complement(22257..22556)
                     /locus_tag="VW191_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU9622875.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(22546..23295)
                     /locus_tag="VW191_000021"
     CDS             complement(22546..23295)
                     /locus_tag="VW191_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEU9622876.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(23349..23708)
                     /locus_tag="VW191_000022"
     CDS             complement(23349..23708)
                     /locus_tag="VW191_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEU9622877.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(23710..25110)
                     /locus_tag="VW191_000023"
     CDS             complement(23710..25110)
                     /locus_tag="VW191_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEU9622878.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSIRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTIFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(25405..25644)
                     /locus_tag="VW191_000024"
     CDS             complement(25405..25644)
                     /locus_tag="VW191_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU9622879.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(25676..26146)
                     /gene="ssbA"
                     /locus_tag="VW191_000025"
     CDS             complement(25676..26146)
                     /gene="ssbA"
                     /locus_tag="VW191_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEU9622880.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(26158..26448)
                     /locus_tag="VW191_000026"
     CDS             complement(26158..26448)
                     /locus_tag="VW191_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU9622881.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(26601..27947)
                     /gene="asnS"
                     /locus_tag="VW191_000027"
     CDS             complement(26601..27947)
                     /gene="asnS"
                     /locus_tag="VW191_000027"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU9622882.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(27963..28328)
                     /locus_tag="VW191_000028"
     CDS             complement(27963..28328)
                     /locus_tag="VW191_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9622883.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(28321..29508)
                     /locus_tag="VW191_000029"
     CDS             complement(28321..29508)
                     /locus_tag="VW191_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9622884.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAVVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(29505..29993)
                     /locus_tag="VW191_000030"
     CDS             complement(29505..29993)
                     /locus_tag="VW191_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEU9622885.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGISQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            30314..30862
                     /locus_tag="VW191_000031"
     CDS             30314..30862
                     /locus_tag="VW191_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9622886.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQSVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(31100..36781)
                     /locus_tag="VW191_000032"
     CDS             complement(31100..36781)
                     /locus_tag="VW191_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEU9622887.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETNPAAT
                     TDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDRGYSG
                     FRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVGNRVEF
                     SIAYSGPGEFVNNNFLLDKGNGFGEPSRATISTNRLRDQTKDIRKGANFVSHSSYSLT
                     SAVETNSNQTIRFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNYYFRE
                     KPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIYKVHQ
                     LGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFYLDND
                     GNGVYTEGVDELVQKIAIKDGAKGEKGVQGERGLTGAQGQAGRDGVTPTVTVKDNKND
                     GTHTITINDGRGNVASTVVRDGFDGASPLVATQRNEADKTTTVIFYYDQNGNNELDAS
                     DKKLKEVIIADGAKGEQGLQGRDGVQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGK
                     SLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGK
                     NGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTT
                     IRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKG
                     PKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGK
                     DGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTD
                     ITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKY
                     TPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLA
                     VIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGAIGQDG
                     QTPTITTQRGQDGQSTVVTITTPGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIR
                     RATDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTK
                     DNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKET
                     VVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDG
                     SHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTV
                     RDGKSPKVTITDEQNGTHKISVLNGDGTTTETIIKDGKSPVATVRDNQDGTYTIRVEN
                     GNGTVSETTVRDGKSPTAKVVDNGDGTHTITVVNSDGTTTTTTVRDGREPKLEVIDNN
                     DGSHTIKVTGADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGK
                     DGKDSVSPTVTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHT
                     ITIINSDGTVTKTVIKDGKDGRDGRDGRDGKDGKDGKCGCQDKPVTPSNDKPVPPTPN
                     VPTPEVPVKPVPAQPTPNVPTPEVPVQPTPAVSTPEVPVKPVPAVPEQPVVPTPAQPA
                     TPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKED"
     gene            36997..38481
                     /locus_tag="VW191_000033"
     CDS             36997..38481
                     /locus_tag="VW191_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9622888.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELTQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            complement(38532..39260)
                     /gene="yaaA"
                     /locus_tag="VW191_000034"
     CDS             complement(38532..39260)
                     /gene="yaaA"
                     /locus_tag="VW191_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEU9622889.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(39339..39503)
                     /locus_tag="VW191_000035"
     CDS             complement(39339..39503)
                     /locus_tag="VW191_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEU9622890.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            complement(39682..41277)
                     /locus_tag="VW191_000036"
     CDS             complement(39682..41277)
                     /locus_tag="VW191_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9622891.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVEGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            complement(41289..41699)
                     /locus_tag="VW191_000037"
     CDS             complement(41289..41699)
                     /locus_tag="VW191_000037"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9622892.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETTRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            complement(41815..42606)
                     /gene="yghU"
                     /locus_tag="VW191_000038"
     CDS             complement(41815..42606)
                     /gene="yghU"
                     /locus_tag="VW191_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEU9622893.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            complement(42619..45315)
                     /locus_tag="VW191_000039"
     CDS             complement(42619..45315)
                     /locus_tag="VW191_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEU9622894.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            45619..46803
                     /gene="mntE"
                     /locus_tag="VW191_000040"
     CDS             45619..46803
                     /gene="mntE"
                     /locus_tag="VW191_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEU9622895.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(46946..48817)
                     /locus_tag="VW191_000041"
     CDS             complement(46946..48817)
                     /locus_tag="VW191_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9622896.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQN
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTAGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(48814..49998)
                     /locus_tag="VW191_000042"
     CDS             complement(48814..49998)
                     /locus_tag="VW191_000042"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU9622897.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHNPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(50010..50777)
                     /locus_tag="VW191_000043"
     CDS             complement(50010..50777)
                     /locus_tag="VW191_000043"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU9622898.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(51054..51902)
                     /locus_tag="VW191_000044"
     CDS             complement(51054..51902)
                     /locus_tag="VW191_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9622899.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            complement(51904..52278)
                     /locus_tag="VW191_000045"
     CDS             complement(51904..52278)
                     /locus_tag="VW191_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEU9622900.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(52352..53704)
                     /locus_tag="VW191_000046"
     CDS             complement(52352..53704)
                     /locus_tag="VW191_000046"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEU9622901.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKKTGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(53728..54507)
                     /locus_tag="VW191_000047"
     CDS             complement(53728..54507)
                     /locus_tag="VW191_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEU9622902.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(54494..55351)
                     /locus_tag="VW191_000048"
     CDS             complement(54494..55351)
                     /locus_tag="VW191_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEU9622903.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            55487..56455
                     /locus_tag="VW191_000049"
     CDS             55487..56455
                     /locus_tag="VW191_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9622904.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(56489..57400)
                     /gene="whiA"
                     /locus_tag="VW191_000050"
     CDS             complement(56489..57400)
                     /gene="whiA"
                     /locus_tag="VW191_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEU9622905.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(57397..58374)
                     /locus_tag="VW191_000051"
     CDS             complement(57397..58374)
                     /locus_tag="VW191_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEU9622906.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(58371..59261)
                     /gene="rapZ"
                     /locus_tag="VW191_000052"
     CDS             complement(58371..59261)
                     /gene="rapZ"
                     /locus_tag="VW191_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU9622907.1"
                     /translation="MTKKQFHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(59313..59693)
                     /locus_tag="VW191_000053"
     CDS             complement(59313..59693)
                     /locus_tag="VW191_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU9622908.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(59704..60291)
                     /locus_tag="VW191_000054"
     CDS             complement(59704..60291)
                     /locus_tag="VW191_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEU9622909.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(60300..61532)
                     /gene="clpX"
                     /locus_tag="VW191_000055"
     CDS             complement(60300..61532)
                     /gene="clpX"
                     /locus_tag="VW191_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU9622910.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(61564..61734)
                     /locus_tag="VW191_000056"
     CDS             complement(61564..61734)
                     /locus_tag="VW191_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9622911.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(61734..62240)
                     /locus_tag="VW191_000057"
     CDS             complement(61734..62240)
                     /locus_tag="VW191_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU9622912.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQDQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(62370..62888)
                     /locus_tag="VW191_000058"
     CDS             complement(62370..62888)
                     /locus_tag="VW191_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEU9622913.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(63385..64890)
                     /gene="lytC"
                     /locus_tag="VW191_000059"
     CDS             complement(63385..64890)
                     /gene="lytC"
                     /locus_tag="VW191_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEU9622914.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            complement(64928..65686)
                     /locus_tag="VW191_000060"
     CDS             complement(64928..65686)
                     /locus_tag="VW191_000060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU9622915.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(65784..66461)
                     /locus_tag="VW191_000061"
     CDS             complement(65784..66461)
                     /locus_tag="VW191_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEU9622916.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLIKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            complement(66470..67414)
                     /locus_tag="VW191_000062"
     CDS             complement(66470..67414)
                     /locus_tag="VW191_000062"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEU9622917.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(67596..68108)
                     /locus_tag="VW191_000063"
     CDS             complement(67596..68108)
                     /locus_tag="VW191_000063"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEU9622918.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(68195..68953)
                     /locus_tag="VW191_000064"
     CDS             complement(68195..68953)
                     /locus_tag="VW191_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9622919.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            69161..69307
                     /locus_tag="VW191_000065"
     CDS             69161..69307
                     /locus_tag="VW191_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9622920.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(69454..70584)
                     /gene="ugpC"
                     /locus_tag="VW191_000066"
     CDS             complement(69454..70584)
                     /gene="ugpC"
                     /locus_tag="VW191_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU9622921.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(70867..70952)
                     /locus_tag="VW191_000067"
     tRNA            complement(70867..70952)
                     /locus_tag="VW191_000067"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70916..70918),aa:Leu,seq:aag)
     gene            complement(71034..71609)
                     /locus_tag="VW191_000068"
     CDS             complement(71034..71609)
                     /locus_tag="VW191_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9622922.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(71609..72397)
                     /gene="codY"
                     /locus_tag="VW191_000069"
     CDS             complement(71609..72397)
                     /gene="codY"
                     /locus_tag="VW191_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEU9622923.1"
                     /translation="MAHLVEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            complement(72661..74220)
                     /locus_tag="VW191_000070"
     CDS             complement(72661..74220)
                     /locus_tag="VW191_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9622924.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNTLSEKQ
                     TGFVIRNKGDK"
     gene            complement(74419..75645)
                     /locus_tag="VW191_000071"
     CDS             complement(74419..75645)
                     /locus_tag="VW191_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEU9622925.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            75839..77155
                     /locus_tag="VW191_000072"
     CDS             75839..77155
                     /locus_tag="VW191_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEU9622926.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            77254..78597
                     /locus_tag="VW191_000073"
     CDS             77254..78597
                     /locus_tag="VW191_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU9622927.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            78597..79379
                     /locus_tag="VW191_000074"
     CDS             78597..79379
                     /locus_tag="VW191_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEU9622928.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            79505..80587
                     /locus_tag="VW191_000075"
     CDS             79505..80587
                     /locus_tag="VW191_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9622929.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTIFETAEFENLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     IIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            80580..80783
                     /locus_tag="VW191_000076"
     CDS             80580..80783
                     /locus_tag="VW191_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9622930.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(80911..81533)
                     /locus_tag="VW191_000077"
                     /pseudo
     CDS             complement(80911..81533)
                     /locus_tag="VW191_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        23333 a        18339 c        15086 g        24775 t
ORIGIN      
        1 gcgcttttcc ttttctaggg taaagcagat cctgtttgct taaccccaat tttccatgat
       61 gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccatc accatcattt
      121 caggcgaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc ttggtcacgc
      181 ttgatttctt gaccgagcgc ttgcgattgt tttcataaga ctgttgagca tagtcggcag
      241 aataaacctc tttgaagcgc ccttttccaa gacattttcg gactgtccta cgcttgattt
      301 tagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta tttgattttc
      361 cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt ttaccttttg
      421 tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac aacaagtata
      481 acacagaggt gttttcttat gcctacaaga gctttcatta tttccatttt cttttggatt
      541 tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt acctgtaaat
      601 ttaatgaaag caacacaaaa tccgagaggg gaatctcgga ttaatagata gagagttttt
      661 agtttaaata aattgtttaa aatatcaaca acatcacttc ttttcttaac ctgataagtc
      721 ttgattccta attttggggc tacgattata ttgtcctcaa tatcgtctag aaagacacaa
      781 tttctaggtt ataactggta tttatcaata gttactcata tacatcagtc cacctccata
      841 cttatgtgcg agcctctctt tgtattatac ctccatactc accttacaga ttcttttggt
      901 aataatatct ttgcctaatg tagagatagt cttgcaaaga aaaaacttcc ttgtagccat
      961 gtttctgata aaagtccggt gcctggaact ggtaagtatt gacaaaggca aaacaacaat
     1021 ttcgattctt agcttcactt tctgcctgtt gcaatagttt tgaaccgatt ccttgccctc
     1081 gcagttcctc ttttacaaac aaatactcga tttctagcca atttccaaaa gtctctgcta
     1141 tcaaacctgc caggagattg cccttttcat cttcgacata aagattaagt ggctcacttt
     1201 cagcctcttc tctttttgaa cggttataaa cacgaatcag attccctatt tcttgcgatt
     1261 tatgtgattc cttattttcc aatctaaagt atagtgaaat gaaataaaac atgcgcaaat
     1321 cgattaagga atttaatcta atttctaaca atgtcttaga aatcaaagtg tactatttta
     1381 acttcaatgc actatacatc taatactcaa taaaaatcaa agagcaaact aggaaactag
     1441 ccgcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa gtcagtaacc
     1501 atacatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag tagcaaaatg
     1561 gaaaaaggag tgagtgaagc acatcgcctc cccactcctt tttctgtttt taggctgttt
     1621 tttcaacctt caagattttt acatcatagc taccaacagg cgtttcaatg gttgctgtat
     1681 cacctgtttt cttgccaatc aaggcctgcc caattgggct ttcatttgaa accttacctg
     1741 caaaggcatc cgcaccagct gaacctacga taatataaac ttcttcttcg tcctcaccaa
     1801 tttcttggat ggtgactgtt ttaccaatcg ctacttcgtc ctgggcaact gcgtcgctat
     1861 tgacgatttc agcatagcgg atttttgttt ctaagctaga gatttgtcct tcgacaaagg
     1921 cttgttcatc cttagctgct tcgtactcac tgttttctga aaggtcaccg tatgaacggg
     1981 caatcttaat gcgttctacc acttctggtc gacgaaccaa tttcaattct tctaattctt
     2041 tttcaagttt ttccttttcc tcaagggtca taggatatgt tttttctgcc atttttctca
     2101 actttcttct gataatattt tctaaagaaa attatgtgaa gtatcacata attttagttt
     2161 gtttagttta atttgctgtt gacatgttca gcgacattgc ggtcgtggtc ttcttgattg
     2221 ttagcatagt agaccttgcc ttctgtgaca tctgctacaa agtagaggtt atcgctctta
     2281 gtttgattga tgcttgactc aatcgcatcc agacttggac tatcgactgg accaggcatg
     2341 agacctacat ttttataaac attataaggt gaatcaatgt tggtatcaat cgcaacatcc
     2401 tcagctagac tgatattttg ccccagtttt ccttgggcat acaagattgc aatattactt
     2461 tgaagtggca tatcacgatt caaacgattg tagaatacac ctgcaatgag cttacgatct
     2521 tctgtcttgg caccttcttt ttcgaccaag gaagcaatgg tcaacaactc attgacagtc
     2581 aagtttttag atttgatagt actatagtaa ggagataggt tcttatccat agcagctaac
     2641 atctcatcaa tcaagctctc aatagttgtg ctttccttga tagagtatgt agctgggaaa
     2701 aggtatcctt ccaaacgata acgcgcaccg ctgtctttta caggcaaact ttccagtaaa
     2761 gtaggatatt tcgctactgc ttgactgata aacgtctcat cttgaacttt agccaagaaa
     2821 gcctccgctg tcaaagactc tttgaagtca ccttgcaatt gacccacagt ttgagcaatc
     2881 tgatccaagg tataaccttc tggaattgtc aaagtcgcaa gtacaggttc ttgcggttca
     2941 tctgttccac ctttttgcaa ctctttgagt aagtcttctg tactcatact cttttgcaaa
     3001 ttgtagtaac ctgctttcaa gtcggtataa tttttatact tggcataaaa actaaaaatc
     3061 agaccatgct ttaccaaacc agctttttca agcgtcgtac cgatttcttg aacgtttgaa
     3121 ccttctggaa ttccaaccgt cacatatttc ttagaattag catcgatagg taataaggaa
     3181 tctagcacgt actggtaacc aaagtaacca cctgctgaga gcaaggcaag gaatacaagg
     3241 aatgaaatca caaaagcttt cacatgtgac ttcttctctt tcttaggctt aacgggtttt
     3301 gctccttctc tacggctacg acgtgatgta tctctgatga tatccactgt ttctgtagca
     3361 ggcgtaggtg tttctggacc tgcttgttct gcttttttct ctgccgtcgt ataggaaaca
     3421 gctacccttg ttggggtttc attgtattct ctttcaagtt tcttaggtct aacaggacct
     3481 ggacctggtc ttgatccact ttcttccgct ggagaagaag gtacatcttg acttggatga
     3541 cttggaacac caggagtttc tctttgaatc tcatctgctg gagaagctgg tacaccttga
     3601 cttgggtgag taggcacggt aggagctttt ctcataatct cctctaccgt tgacaaggaa
     3661 tcagccatga gttcttcagc tgaaggttca tttgcaggag tgcgaactac tgcctcatct
     3721 tctttcagaa cttcatcata gccttttact ttttctaaat ctctcagaat ctgctcttta
     3781 aagcttaatt tctcttcttc tcttgacttt tcactcaaaa gtttttcctc cttgttgata
     3841 atccataata ttatatctta aaagtccaaa aaaagcaatc tatgatactt ttcctaactt
     3901 attttttcat ttcccagacc atatcatacc atgtttcccc tgcaaaggtt gactgggact
     3961 ggccttcgtt gacaaatcca tgtttttcat aataggcgat gagatagtca tgacaggtta
     4021 gattaatgcc atctctctca tcttcaagag caacttcttt caaggctgtc agcaatttct
     4081 gacccagtcc gagtccctgt gcctccttgg caatagacag acaggtcaca gagatataac
     4141 caccaggctc atgactatag tcttttattt cttctgtaaa agactggtct tgcagatggc
     4201 ggtgcaggcc aactggtcct tcgatataac ccatgattct tccttctttt tcagcaacca
     4261 gaaaagaggt ctgaatttct ctcaaatgtg cttcaaagac agaaggagga atggcttctt
     4321 cgaccgaaaa attttcaaat tcaagttcaa caatccgatc caaatcttct aatcttgctt
     4381 gtctgatttt cattgttcct ccagataaaa gggattaaac caaatcatac tatagccctg
     4441 gctagttgca tagagcaaag tttcttcttc atcaacaaaa ccgttcattt caaaatagga
     4501 aagcagctca tcaggactct ccaaacgaat ccctttgtaa tccagctcaa ctgccacctc
     4561 tttcaaggct gcaagaagaa gtgttcccag gccctgtctc tgatggtcag actcgatgac
     4621 taaagaatgt acttttagac attgcggatt gtctgactgg ggacttgata aaatatagcc
     4681 taaaagttga ttttcatccc tagctagaag aaaggtatcc gcacacttac ggatactttc
     4741 ttctaaaata tgggaaagtt gctgcttttc agctggaaaa gacgaggtct gaagtgcccc
     4801 tatctcaggc aaatcaaact tgcttgcctg aatgatctta attggaattt ccatgggaac
     4861 atcctattga acattgcttg tcaagttaga caagagacgc tcaaatgagt attcataggt
     4921 ttggatgtct cctgctccca taaagacgta aacagcattg tcatggtcta ggagtggaga
     4981 aacattttca acagtaatca cttggtgttt tttgttgatt ttattggcta ggtcttctac
     5041 cttaacgtca ccatgatcta cttcacgagc cgagccataa atttgcgcta gataaacagc
     5101 atctgcttgg tttaaagcat gggcaaagtc gtccaacagg gcaatggttc ttgtaaaggt
     5161 atgcggttga aagactgcta caatttcctt gcttgggtat ttctgacgag ccgcatccaa
     5221 ggtcgcaata atttctgttg gatgatgggc aaagtcatca ataatcactg tatcattgac
     5281 aattttctca gtgaaacgac gtttaacacc ggcaaatgtt ttcaagtgct cacgcaccaa
     5341 gttcaaatca aatcctgctg tgtaaagaag accaataacg gctgtcgcat tcatgatatt
     5401 gtgacgacca aaggttggaa tgtggaattg ccccaagttt tgtccacgga aatgaacggt
     5461 gaaggttgaa ccagttgttg aacgaagaag atcactagct acaaagtcat tgccttcagc
     5521 ttcaaaacca taataataaa ttggtgcatc agacgtaatc ttacgcaatt cagcatcttc
     5581 accatagaca aaaagaccct tggtaatttg tttggcatag tcgttaaagg cattgaaaac
     5641 atcctcgaga cttgtgaaat aatctggatg gtcaaagtca atgttggtga taatagagta
     5701 ttctgggtgg taaggcatga agtgacgctc atattcgtca gattcaaaga caaaatattt
     5761 ggcattagcc gaaccacgac ctgtcccatc tccaatcaag aagctggtat ctgtaatgtg
     5821 agacaagaca tgagacaaca tacctgtcgt tgaagttttt ccatgtgctc ctgctactcc
     5881 catgctaaca aagtcacgca taaagctacc tagaaactca tggtaacgtt tgtagctgat
     5941 accattttgg tccgcatagg caatttcgac gttgttatct ggacgaaagg catttccagc
     6001 gataatttcc atatcaccgt ctagattctt ttcatcaaaa ggaagaatgg taattcctgc
     6061 ctgctcaaga ccgcgttggg taaagtagta cttttcaaca tctgatccct gaaccttgtg
     6121 ccccatctgg tgcaacatca aggccaaggc actcatccct gatcccttaa ttccgataaa
     6181 atgatatgtc tttgacatgt tttctcccct attctgtcat tctggtcaga ttcaactctt
     6241 gggcaacccg acgttcttgt tctgtttgtt tacttttttt attgtagatt tggctcttct
     6301 ttagaaaatc ataattgttt ttctttggag caggtgctga cacttcttca ttcttggtag
     6361 ggatagaatg aacttcttcc gccaagatat aatgagactg ggtcaatttt tggctatatt
     6421 tgacaaattc accaggattt tccttttgga aaggagttgt cggttgattg ccctgtctaa
     6481 ctagactggg ctgagaatga cgtctcgcaa ggctgaaatc ctgagttagg tagttagcag
     6541 agcgtttctt tttcaagtcc gcacgcgctt cttcacgcgc cacctccgca tagctctttc
     6601 cttctttttt aacccctaaa ggagcctttt taggtttttc gacttgcttt tcaatcggtt
     6661 ttactggttt ttcttcagta ataggagccc attctaaata atttttatct cgatactcac
     6721 ccttgatatt actgatcaga tcagactcat catagagatt catgactggc atttcagtca
     6781 acatgacctc gtcatctgac accaatggaa atcgttcttg tttcattttc tatttccttt
     6841 caacacttca ttatagcgta ttgtcttgat ttttcaagtg ctggcttcag aaattcccaa
     6901 aatttctcta atttctgcta gggtcagact accacgtgac tctgtgccat ccaatacttg
     6961 tgacaccaga tgtttctttt gttcttggag ttcctgaatt ttttcttcaa tggttccctt
     7021 ggtcaccaag cgatagacct caaccgtttc ttcctgaccc atccgatggg cacggccaat
     7081 ggcttgcgct tccaccgcag gattccacca aaggtcaacc aagatcactg tatctgcacc
     7141 tgtcaggttc agaccgaccc caccagcctt gagggaaatc agaaaggcat ctctttctcc
     7201 ttggttaaag gccttggtca tgtcttgtct ttccttggct ggggttgaac ccgtaatttt
     7261 aaaggatgtc aggcccaagt ctggcagttc ttgttcaatt ttttccaaca ttcccttgaa
     7321 ctgagagaaa atcaagacac ggtgtccgcc gtctgccacc tgtaccagta gatctcggag
     7381 actatctagt ttgccgctgg ctccctgata atcttccata aacagggcag gagtgtcaca
     7441 tatttgacgc aagcgcatca aaccagataa aatttccaca cgacttcgct gaaattcctg
     7501 ttctgacact tgagccagat ggtctcgcat ctgttgtaac tgggcaaggt aaatagcctt
     7561 ttgctggtct tccagttcat ttttataaac cacctcaatc aagtctggca attcagtcag
     7621 aacttcttct ttcttgcgtc gcatcacgaa aggcttgata aactgagcca ctcgctctgc
     7681 tggcaatttc ataaattctt tcttgcttgg caaaagtccg ggcatgacga tttggaaaat
     7741 agaccacaac tcacccagat ggttttcaat cggagttcct gacaaggcaa agaccgacgg
     7801 caccacaaat tgtctcaagg tctgggcaat cttggtctgg gcatttttca tgacctgagc
     7861 ctcatctaag aaaaggaagt caaaggccat cccctgataa aactcactgt cctgacggaa
     7921 ggtggcatag ctagtcacat agatttgatg gctctcggca agaatctcct cacgacttgc
     7981 tttcaaacca tgaacaacag ccacatccaa ctgtggggca aatttctgaa actcatctgc
     8041 ccagttgtaa atcaaacctg acggagcgag aatcaaaacc cgactttctt ttgtcacttg
     8101 actagtcaaa aaagcaatgg tctgaagggt tttcccaagt cccatatcat cagccaaaat
     8161 cccaccaaaa ccataatgat ggagcatctg caaccagcca attccctttt cctgataatc
     8221 tcgcaagtca gcctgaacct gagttgcctg tcgaggaaag tcctctggat gtgtcaaatc
     8281 ctgggccaga ttctggaatt cttgtgaaaa agaaacacgg tctcgccctt caaagagatg
     8341 agctaaactg taggccaagg atttccgagc ctgcaaggtc ccatctttta attcaaattg
     8401 ccccagttcc tgtagatttt ggcgaatttt cttggtttct tcatcgaaaa agtaaacttg
     8461 attagacgaa tcaatataaa aatcctgatt ggcaaccaag gcctgcatgg cttggtcgat
     8521 ttcctcctgg gcaatatctt gaaaatcaaa ctggatttcc aagagacctc ccttggaggc
     8581 aatctgcacc tgaggactcg ctaggctata aagctcttct agtttatctg ataggtcaac
     8641 atgcccgagt ttttcaaaga ctggaatgat atcatgaaaa aaatgataga cagactccgc
     8701 ttttaaggcc tgacgccaag attgaaaatc ggcttcaaag cccgcagcca aacagacttg
     8761 gaaaattctt tcttctaagt ccgcgtcact tgaaaatggt aattcttcta gctcttgtcg
     8821 gctagatacc tgtctatttc cataatcaaa ctgaatttct aaacgaatcc gattatcttc
     8881 ttccctgtca aagtaaaaag agggcgcaaa agttttgatt tgtagacgtt ctggagctga
     8941 aacggtgccc atctggataa aaagagtcag acaggaggcc aatttgtctc gatcactgct
     9001 atcaaattgc aggtatttct ttccttgttg acccacaggt aacgctttaa tttccttgag
     9061 aagacgcatc tgctggtctg ttaaaaaata aacctgacct ttatggaaaa gtactgctcc
     9121 ctgataaaag acattgaccc taggactctc actgatttcc atttcaaaat agtccgagta
     9181 ttctgttact gtaaaggcaa atagattggc atcagcatgc atatcctgaa aaagcagggt
     9241 ttggtagcta tccacttgat ggtcaaattg aaaatggggc aaggccatca gtaaattcac
     9301 accctgctca aaaaaggtca gagggaaaaa gaggtgccga ccttggtttt ggaaaaagag
     9361 gtctgaaacc agcccttcct ccgttagtcc gtgcaagaaa gtcaaaagtt cttggctggc
     9421 atcatcaaag gcttcccaag aaagagactc ctcataaatc ttgccaatca tatacgactt
     9481 tctctgctcg acaatcctta aaaaaagtgg aatatcccga atgacatagt atttttggct
     9541 attgatttgg ccgattctca gagtccacaa gatatgattg gttcctgctt ccacctgacc
     9601 cacagctgat aactcatagg cgcattctga ttttggagat aaaattcgat ccaaaaactt
     9661 gccacccaag gtcaccttgg tttcaacagc ctctttttct tcatgacctt cttccagact
     9721 ccacaagatt tcctgaccac gctcatcatt tttcagaaaa tgctctagcg ctgccaaatg
     9781 cacacagtaa cccctctttt gaaaaaaatc acaggcacaa aaaaccaaat catcctctaa
     9841 actatagcgc agttcttctt ctgcaacgcg agcgtagagc cgattgttct tttccttgat
     9901 gatatcaacc ttaccagttt cataaagggc aacaccttcg atacgaactt tccccggaat
     9961 caatttagcc atattttcac ctttacctta tctttttatt ataccatatt ttcgcctatg
    10021 aaaatagcct tctaggaaga cttttctcct agaagactgg atttttaacg tttggcaaaa
    10081 gtagccacaa tccgctgaca gacttcctgc aacagagatt tgggcatagc tatattgatg
    10141 cgggcatgga gacttccttc ctctccaaaa tccaaaccac ggttgaggat aaccttggat
    10201 ttatttctca acaactcttg caatgtttca tcagtcaggt cataggctga aaagtcaagc
    10261 caaatcaagt aggtaccttg cggtttcatg accttgattt tagtctcttt tccaaataga
    10321 tctaccacat aattgatgtg gtcttcaaag acttgcttga gttcctctag ccaatcttta
    10381 ccgtatcgat aggcagcttc tgtcgccaaa taacccaagc ctgaaatttc atgctgatta
    10441 ttggccaaca ggcgtttctg gaaagccagt ctcaacttag gattttcaat gactgcatag
    10501 gaattttttg ttccagcaat attaaatgtt ttagtggcac tgctcaagac gatagcaaaa
    10561 tttttgaagg caggattgat ggtattgaaa gactggtgtt tgtgaccaaa gagggtcaaa
    10621 tcttggtgaa tctcatccga aactaacaaa acaccgtgtt tttggcagag ttggccaatc
    10681 ttctccaaca cttctttttc ccaaacacgt ccaccaggat tgtgagggtt gcaaagaata
    10741 tagagtttaa cctcctcttc caccaaatcc ttttcaagtt ggtcaaagtc aatctcaaac
    10801 agaccatcct tttccactaa ggaattagta atcaatctac gattattcaa cttgacactg
    10861 cgagcaaagg gtgggtagac aggcgtgtta attaaaaccg cctcgccttc ttttgtaaag
    10921 gtttgaatag ctgttgagat ggctggtacc acaccctcga taaagacaag agcctctttg
    10981 tcaaagttgt aaccgtattg tgtagcttcc cacttttgaa cttccttaat taagtcttca
    11041 ctggcatagg tataaccata aaccagttgg tctgcgtaag tttgcacggc ttggcggatt
    11101 tcaggcaaga ccacaaagtc catatccgct atccaagctg gtagaacttc actatccgtt
    11161 tctgtttctt tccatttata ggtatggtgc cctaaacggt tgggcaggct tgtaaaatca
    11221 tattttccca tctttgtctt atccttctat ggcttggcgc aaatctgcaa tcaaatctct
    11281 agcatcctca atcccaatag acaaacgcaa gaggtcatct gtcaaaccat aagaatggcg
    11341 tacctctgct ggaatatcag catgagtttg agtcgttgga taagtaataa gactttccac
    11401 tccacccaaa ctttccgcaa aagagaagag cttgagactg ttcaaaatat gaggaatgcg
    11461 tgtttcatcg gctactttaa aggaaatcat gcctccacga ccagtgtaga gaacttcctt
    11521 aactgctgga gaatccttca aaaaggcaac cacttcttgg gcgttagctg ttgagcgctc
    11581 catacgaaga gacaaggtct tgagaccacg aagcaactgg tagctgtcaa atggagacaa
    11641 gactgcccct gttgtattaa gattgtaaaa aagcttctcg tatagttcta aactattggt
    11701 cacaaccact ccagccaaga catcattgtg gcctgctaga tacttggttg ctgaatggag
    11761 aacgatatct gctccatctt caatcggacg ttggtagata gggctataga aggtattgtc
    11821 caccaccact ttggcaccct tagcatgagc caattttgct agtttttcga tatcaaattc
    11881 caacatcaag ggattggttg gggtttcgat atagagaaca tccacatcct tttctaactc
    11941 ggcaatcaac tcttcttctg tattggcata ggtaaaatgg aaacgacctt cctgctccac
    12001 ttggttaaac cagcgaaaag aaccgccgta aagatcacgc actgccaaga ccttacttcc
    12061 tactggaaag ccgctaaagg ccagtacaat agctgacatc cctgagctag tcgctaaggc
    12121 atagtctgct gactcaatag tcgccaagac ttcctcagcc ttactacgag ttggattttt
    12181 agtgcgcgta tagtcaaacc cagtagatcg accaaactct ggatgctgat aggtcgttga
    12241 aaaatgaagc ggtgtcacca atgcacctgt cgcttcatct gacttaatac ccgcctgggc
    12301 caaaattgtg ttaatgtgta attccttgct catacaatac ctccaaatct atagtaacta
    12361 ttgtaccact tattttcatc aactcatttt cttcttttca agagctagtt atagtttcaa
    12421 actatataaa aagggagttt ttcctgctcc cttaaataga ctataaaatg gtgaatctca
    12481 aaagacactt cactctatca tttgctcctg cacaaaacga gcataacgct catgattttc
    12541 cagtagttcc ttatgagttc ctgagccagt gattttcccc tcctctaaga agaaaataca
    12601 atccacatct tttaccgttg acaaactatg cgctataatc acaaccgtct tctcctttaa
    12661 tacagaatag aggctactga taatcgcata ctcagaatcc gcatcaagat tagcagtagc
    12721 ttcatcaaat ataagaattt cagcatcttt taagtaggtt ctagctattt gaagtctttg
    12781 gctttgcccc cctgacaaga atcgtccgcg ttcaccaact tcagtatcta gtccctcttt
    12841 catggagcga atctcatcac ctagtgatac taagtctagc actttcatca attcatcatc
    12901 agttactaag cgattcaaac cgagacaaag attgtcacga atactgccag ataagactgc
    12961 attattttgt gaaacccaag cgattttact tctccattct tttaagttaa aatcatatat
    13021 acttgattgc tccattagaa tatctcctga aagcggttta taaaaccgct ctaacaaacg
    13081 cacaatcgtt gattttcctg atccagatgg tccaacaaaa gcaatttttt gccccttgaa
    13141 aattgaacaa gtaatatcct ttaagacagg tcgattttca tcataaccaa aatagacatg
    13201 gttaaaattc aacccttgtc ctgataccga ttttcctccc tcaaattttt ctttaggaac
    13261 tgcaagcaag ttctccagtg caactgaaga tcccttgctc ctagaataaa cagttacaaa
    13321 attagctata ttactaatag gattaagtaa ttgaaagagg taaatcaaaa acgaaaccaa
    13381 ggttcccaca gatatatatc ctgcgctgac ccgataaccc ccataggtta gcatcacagc
    13441 tatagccgca aagataaata agagagcaaa cgaggtctca aaagaagtaa ccctatctga
    13501 tttcagtgaa ttgttttgta ccctttcaat acaattatcc aaaacatcct gtacactttt
    13561 ctctgcttgg ttagtcttaa ttaattcatg ttcttgaatc ttttcagtca attgccctgt
    13621 taaaaatccg atttcctcct gtaaacgacg actatacttt tcactgatat tggaaagggg
    13681 caagataata aacatcatac aaggaagagt gatgaataaa agtagagaaa gattccaatc
    13741 aagactaaat aagactacaa tggaaccaag taccataact aaactcagaa taatatttgg
    13801 gaaagtcgta attaaaaact cacgaatgac actcgtgtca ttgacaatgg cagaagtcaa
    13861 ctccccactt tagctcttat caaagaagga tttctctgca taaatcaacc cctctatcac
    13921 ttttttcctg atttttgcta tctttttttc acccgattga ctaaacagat agtaaccaat
    13981 agaagaaaac aaggcttgac caataaaaat caaaaacgat tgaaatactt tggaacctat
    14041 attttcaata gaactcccat ctattaaacc ttttaagata aggggaagca acaaagcaag
    14101 tagactagac agaacaagga agaaactccc cataatcacc ttagtatcta ctcttaataa
    14161 ttttaatttc ataaatactc cttataatat ttcaacggat aaagtcggga ataactcaat
    14221 ttgaggataa aatctaataa atcttcctat aacaaaacgc ataacatcta ggattttata
    14281 tacctgatat tatgcgtttt taagcacaaa gacttcttac acaaacttat ctacaattag
    14341 attttatttg acatgttttg ccaattcttc ttgggctttt ttattggatt cttctttttc
    14401 tttcaaccat ttttctctag cttttgcata ttcgtctgtt gtgacaatct tatcttgtac
    14461 tttgaggtat ttatatgatt caatcccttt tgtaccggtt aaaccatagg cagcagcaaa
    14521 tggtacggtt cttctcaatg atggtgttcc cccacgcgaa acacttggaa gaactaaaga
    14581 actatcaatc aaccaagctt gaatatcagc atatttctca taacgtttgg ctggatcttg
    14641 ctctttatta gcttcttcca acatttgagt atagacatcc agtccaactt ccttagcctt
    14701 gtcattggct tctccaggct ctagtccaag attttgtaga actcctccac tattagtatt
    14761 aaaaatatcg agataggttg acgggtcttg ataatcaggt ccccaaccgc catgatataa
    14821 atcatagtct ttctgagcag ctgtttgagc aaagtagcct gaactgtcaa actcatctga
    14881 tgttaattgc tgaatgtcaa tcactacatt atcagaacct aaaacagatt caattgattg
    14941 tttgatagaa ctaactcctt gtatgcctac tttatctgtt acttccacag tcttatccaa
    15001 gtggattggg aattgaacac cctttgcttc gagttctttc ttagcttccg caaacttagc
    15061 cttggctttc tcaggattgt agtaagggtc ttgaccatcc gcaaagttga taccttgcca
    15121 ttccttacca tagttgacca tcttagaggc tacaacttca ccaaagtctt ttcccttgat
    15181 actgacaaag tttggaggaa ccactaggtt acgcaaaatc tttgttgcac cttctttccc
    15241 ttcagactga gccccataag atgttctgtc aaaagcaaaa ttgatagcct gacggaagtt
    15301 tttattgaga actgcttcct gagtcgattt cttttcaatg tcacttgttt tagaagtata
    15361 attgtaagac ttcctatcta ggttaaaatt aaagaaatat gaagttgaat tttgcatact
    15421 atagatgata ttgtttttgt atttttcttt aatcccttca tagctggagc tgttaggaaa
    15481 aagacgagcc gtagtataag caccagctgt aaaattacgt tccagtgatt cttggtcgct
    15541 accatcatag taggtcaatt tcacatcgtc tacaaagaca ttcttagcat cccagtaatt
    15601 agggtttttc ttatattcaa tagcagattt tgagacaagt gctttcatca agaaaggtcc
    15661 attgtacaaa atactagatg gatccgcctt cccaaaatca tccccttttg atttcaggaa
    15721 atctgcatta acaggaaaaa gtatcgttgc aagtgttttt gaattccagt aaagttctgg
    15781 tttaaccaaa gtatattgaa ccgtttggtc atcaagtgcc ttgacaccga cagttgaaaa
    15841 gtcgcttgtt ttaccagtga tatagtcatc caaaccagca acagagtcct gcactagata
    15901 caaggcttct gattttttat cagctgcata ttgcaaacct gtcacaaaat cctgggcagt
    15961 tacaggcgca tattcttctc cctcagaagt aaaccacttg gcatccttac gaagtttgta
    16021 ggtataggtc aaaccgtcct gagaaacagt ccaatcctct gctaatgatg gaataatatt
    16081 cccatattgg tcattttcta ataacccgtc taccaaattt gcaacaatat cggatgttgc
    16141 tgcgcggttt tctgctagat agttcaagct agatggatca cttgaataaa catagttgta
    16201 tgtttttgac gccgtgctag aatttccaca cgcgctcaat aaaactcctg tacccaggac
    16261 aagacctgcc aaggttagat atttgctctt agactttttc atttccggaa cctcctgctt
    16321 taaatatttg ttttattata caacgtttag tttgtttagt caatgatttt ttccaaaaaa
    16381 tcgaaaaaag cctcattttc tatttttgaa atataatttt agctattttt acgctaaaaa
    16441 aggaataagc ttcaagttct cctaaaagat tgttcaactt attcctttta tttataaatg
    16501 ctaccttacg aaagcttaaa ttttgctcgc aggcgatctg cttgggcttt tcctattacc
    16561 ttatctaata aataggtacg cagactcata accatataaa gtccaccccc catggcaccg
    16621 acaagagcta cataaaagaa gctccacaaa cgtccacttg gttggaagaa aaatcctaac
    16681 agccactgga tggttcctat taacagaaac atgactaggg tcagcaaact gattaaaatg
    16741 gttcgcttca aaatcacctt gcgcttgaca ccagttactt tacaaatatc ccgatacatc
    16801 aagacgttag gaatgatgag agcaatggtt gttgaaatca aaggaccata actgtggaag
    16861 agggcgatgg taggtagttg caagactagc ttggcaatag aaccatagat aaaatagaga
    16921 acggccttgc ggttgcggaa catggcctga aacattggag acaagaccat gtacaagcct
    16981 aaaataatag actgcaaaac tgcaaagaca aataagccca gagccaaact atctggctta
    17041 ccatagaaga ccgtataaag aggttctcct accataacca ctccaaccgt tgctggtagc
    17101 aagaacataa agagtagggt gagactgtcc tgaacgagac gagaagctgc tttcaagtcc
    17161 cccttgacat agttttccgt caaaagtggc aaaccaacac tcccaatcga aacccctaca
    17221 gaaatcaaaa tcatcgtgat tttattagga ttggctgaga aataagaaaa catgacaacc
    17281 aagtcctcat tgctgtagtt ggtaaaccag ctcatactat tgataaaggt cagctgatcc
    17341 aaaatctgga agagctggat ggcagaccct gtcaggataa aaggaatggc ttccttaatg
    17401 gtatcaacca agagacgctt actgttaatc ttatctcctg tttcaaagac tcttttgagt
    17461 gaaccttctt gggcaaggaa ataaatcaag actgcaaaac tggctaccat accgacaaag
    17521 gcagcaaagg ttgattgggt aacggctgct agataatctc ctgaaccgag cttcataatg
    17581 ataaaggttg ctaggagcat ccagataaca cgaatgacct gctcagcaat ttggctcatg
    17641 gcatagggtt tgaggttatt catcccttgg aaaaatcctc ggataacact catagacggg
    17701 aaaatcaaga ctccccaagc caagctttgc atgattggga tcaagtcttt gcccacgcca
    17761 gacaagtctg ctagccaagg agcaaagaca tacaagacta aagcaaaaac caggcctagt
    17821 cctgtcataa agcctaagaa gctccgaatc agggcaaagc tatgctcttc ttctcgcatg
    17881 gtattatact tggcaacttg cttggccacc gcaactggaa tccccgctgt tgaaaccaac
    17941 aagaaccaag catagatatt gtaacccatg gtaaagagac catttgcctt agctgcataa
    18001 gcccccatcc agatgtacca agggataatg taaacagccc cgagtaggcg actgataaag
    18061 ttactagccg ttagccaagc agtcccccgt aacatctggg cctgctggtg attgttttcg
    18121 tgcgacatag attcctcatt caattttgat aactaggaaa aactccttat cttccattat
    18181 aaacttttcc ttgttacttg taaatttgcg actttcggtt tacaatagaa agtatgataa
    18241 aaattgaaac tgtattagat attttaaaga aagatggcct ttttcgcgaa attattgacc
    18301 aaggtcatta ccactacaac tacagcaaag ttgtttttga tagcatcagc tacgacagcc
    18361 gaaaagtaac agaagacact cttttttttg caaaaggcgc tgcctttaaa aaagaatacc
    18421 ttctttctgc tataacacaa ggtttagctt ggtatgtagc tgaaaaggac tacgaagtcg
    18481 atatccctgt catcattgtg aacgatataa agaaagccat gagtttgatt gccatggagt
    18541 tctatggtaa tccacaagag aaactcaaac tccttgcctt tactggtact aagggcaaga
    18601 caacagcaac ctatttcgcc tataacatct tatctcaagg gcatagacct gctatgttgt
    18661 cgaccatgaa cacaactctt gatggcgaga ctttctttaa gtcagcgttg acaacccctg
    18721 agagtattga cctctttgac atgatgaatc aggctgtgca aaatgaccgt acccacctca
    18781 tcatggaagt ctccagtcaa tcctatctgg tcaaacgtgt ctatggtcta acctttgatg
    18841 tgggagtttt cctcaatatc agcccagacc atatcggccc gattgaacac cctagctttg
    18901 aagactattt ctaccacaag cgtctcttga tggaaaatag ccgagcagtc atcattaaca
    18961 gtgacatgga ccacttctca gtcttgaaag aacaggttga agatcaagac catgatttct
    19021 atggtagcca atttgataac caaatcgaga attccaaagc ctttagcttt tcagctacgg
    19081 gtaaactcgc tggagattat gatatccaac tcattggcaa cttcaaccaa gaaaatgcag
    19141 ttgctgctgg acttgcttgt ctccgtctcg gagcaagtct tgaggacatc aaaaaaggca
    19201 tcgctgcaac ccgcgttcct ggtcgtatgg aagtcctcac tcagaaaaat ggagccaagg
    19261 tcttcatcga ctatgcccac aatggggata gtctgaaaaa actcatcaat gtggttgaaa
    19321 ctcatcaaac cggaaagatt gctctggttc tgggatcaac aggaaacaag ggagaaagtc
    19381 gtcgtaagga ctttggcctc ctcctcaatc aacaccctga gattcaagtc tttctgactg
    19441 ctgatgaccc taactatgaa gacccaatgg ccattgcaga tgaaattagt agctacatca
    19501 atcatcctgt tgaaaagatt gcggatcgcc aagaagccat caaggcggca atggctatca
    19561 caaatcacga attagatgca gttattattg cgggtaaggg agccgattgt taccaaatca
    19621 tccagggcaa gaaagaatcc tacccaggag atacagccgt cgcagaaaat tatttataag
    19681 aatagaaaaa atcaagggaa aatgaaaccc ttgatttttt cctattcttt agtaaaaacg
    19741 tttttcaaac cttcaacggc accttctaca gcaccttttg cgtcttctac aacgtctttt
    19801 accttagcaa ctgttttttc aacagctcct tcaagttctg tcttgctgtc tccagtaact
    19861 ttaccaaaac cttctttgac agcgcctgtt gcttgttcca atttgttttc aagtgacata
    19921 tgattgtctc ctttagttta ttccgatatg tgttaccggt tacatatagt ttataccgaa
    19981 tactaaggaa agtcaaacaa tgtactcaga aacaagaaat cacgctaagt agaaagttaa
    20041 tctctcctta tcaaaatcga tagccaactc atactttcca tcttcatttt gcacaatata
    20101 gcctaagaca accaaactgt ccacaaagat atcacggcat ttctgcataa gctggtcttt
    20161 tttgagaaat ttcaacaaaa aagccgtcat atacttgagt gcatactcag gattaacatc
    20221 tcctaaaatg tcatagagct cctgctgttt ttctgtcaaa ggatactgct gtttgacctt
    20281 gtaaaaataa ttggacaggg tcattttaat gcgcgcaaag tccgtctttt caactaaaat
    20341 agctgcattg atttgattgc gcaattccgt ctcaaaactc tgctccaaca aggcttgata
    20401 gaccggacta gcttctctga caaaaatctc ttgatccagt tcaagactat caagtgattc
    20461 aagcgtagga aaattgaggt aatagcgctt attttcacgt agaatcaagc ctgcctttat
    20521 atactcttcc atgagtttat caactgcaac atctggaaat tgagccttaa tttcccgtag
    20581 aatcacatcc tcatactggt ccagatagcg gatcaattct ccaaaaatgg ctgtctcgtc
    20641 aaacgagatg gattaaaaat ctgaatcatg aagattcctt tctttacatt gtaacagagg
    20701 cgatgctgcc aactgactag gattggtacc agcttggttc agaggaacag gcagacctag
    20761 ttctatgtaa tactccctcc aaaaatcact aatctcctgc tccccatctc caaatttcat
    20821 aggaatagtt tcaaaaatag gtaggatttc tgctatatac tgggagcagt agaaagcagc
    20881 tgcatctgga tagaaagaag cattgtaggg tgctccaaga tgtttgcaag ctctttcctt
    20941 caccgactgg atatccattt ctgggtaaac atagaggtcg tataaatgat tggactcaaa
    21001 gaagtctgcc ggttcttgac agacaacacc agcctgtcca ctagcatgat aaatcatccc
    21061 atccaaataa atggcaacat ggctatagtt acctgtggaa gtctggatgg cctgtcccat
    21121 gtctgaccca tctctcacaa aaatcaaatc gccattttct aacatacttc actcctagaa
    21181 aaaaggagcc gaaactcctt tcgatataag gcaaactaga ctttccgatt ttgagctgac
    21241 acccaaaatc ttacgcatta aagctttcag tcaattgagg taccacttgt ttcttacgtg
    21301 aaacggcacc agcaaggaag gcatgattgt tttcaagttt gaagttgaaa gccgcttcga
    21361 ccttgtccat attggcacca agagccaaga tttctgagtt tgagttgacg atatctgtaa
    21421 tcatcaagac aaagtcagag tagccgtttg attcgttggc agcttgcatt gcagcttcaa
    21481 tttctgcttg gcgttccaaa acttcagcga tgtcaactgt gttcacttgg gcaacacgga
    21541 cattatttcc gttgagttca aaagtcttag catcgatgtc aatcaattct tcagcagatt
    21601 tgctagccaa gttggtacca gctttcaaca ttgccaaacc atattcttcc aagttcacac
    21661 cagccaattc agccaattca ggagcaatga ttttatctgt tgggtgtgtt gttggtgatt
    21721 tcaaaagaag ggtatctgaa atcaaacctg aaagcatcaa acctgcaatc tctttaggca
    21781 cagctacacc atattctttg aacatacggt aaacgattga agacgctgat ccaactggct
    21841 ccaaacgcat gtaaagtggg cttgcagttt caaagttagc cacacggtgg tggtctacaa
    21901 caccgtaaac ttctacttca gcgatatctg atacagattg ttggaattca ttgtggtcag
    21961 tcaagataac ttgctctgcc ccctctgctt tggcagaagt gataacacgt ggtgcttcca
    22021 caccaaaata gttcaagaca aaggctgttt cttcatttgg agttccaagg gcaacagctt
    22081 ccgtatccaa accgtaagct tcttttgcaa ggtaggcaaa agctacagat gacccgatgg
    22141 catctgagtc tggattttgg tgaccaaata ctagaatctt ggacatgata atacctcatt
    22201 ttgtttattt tctttattgt agcatttttt tcgctttttg acaaaggtaa gaggagtcaa
    22261 aggactcccc acgattcttc aaaataactg agtaaatttc tatcttgatt ttcagataaa
    22321 aattcttcct tctgtggcct cttcttacgc ttgagaagag cttcagccga catggcttct
    22381 tccttactgg caaaaccttg agcatagata agtttgactg gcaagcgtgc tcgtgtatat
    22441 ttggctccct tcccactatt gtggatagcg aggcgtcttc tcacatcagt cgtatagcct
    22501 atatagtagg atccatcacg acactccaga acgtacatat aagccttatg atccataata
    22561 aatctcttcg atttcgggcg tataagagcc atcatcattg tggacaatca aaggaggtaa
    22621 gaccttaaag ccacttgttg agccatcctt gatcgcctca atcaaaagca tattggcttc
    22681 cttttctctt tttggataaa caaactgcag gcgcttaggg gctagattat gtcgttttaa
    22741 cgtatccaaa atatccagaa gtcgatcagg acgatgaacc atggccaaac gcccattaga
    22801 cttgagaata ctctgggcac tacgacagat ttcttccaaa ttagtcgtga tttcgtgtcg
    22861 agccaagaga taatgttcac tctcgttcag attagaataa ggatccacct tgaaataggg
    22921 tggattacac aaaatcatat ccaccttact cccctgaatg tgagcaggca tatttttcaa
    22981 atcatcgcag atgacctgca tttgctcctc taatccattc aaacggacag agcgttcagc
    23041 catatccgcc aaacgctcct gaatctcaac agacaatatc tgtgcttgag tacgagtgct
    23101 agcaaaaagc cccactgctc cattcccagc acagaaatcc acaatcaacc ccttcttagg
    23161 aaaacgtgga aatcgtgata agagaacact atccaccgaa tagctaaaaa cctctctatt
    23221 ttgaatgatt ttgatatctg tcgaaaagag ctggttaatg cgctctcctg attttaataa
    23281 ttgttcttct tccatggtcc tattatagca aattcatatt aacattacaa aaaatataaa
    23341 actctaaact acttcttctt ttttaaatgg tgcagggctt ctccagtcca gattggtagc
    23401 attcgtcgaa agggagcaaa accgtagtta aagcggtcgc ttgaaaagcg tctccgtcta
    23461 ggaaactggt acttttcttc ctccaaagtg cggatagaaa gactggcttt ccccgtaaat
    23521 tcatctaaat ccactacctg aacttgaacc tcttcatcga ctttcaaggt ttcatgaata
    23581 ttttcaataa atcctgtccg aatttctgaa atgtgaatca gccccgtatc acccgtctct
    23641 aactcaacaa aggcaccgta gggctgaatc cctgtaatac gcccctttag cttatcaccg
    23701 attttcatct tagtcctcga tttcaatagt ttcaattaca acatcttcaa ctggcttgtc
    23761 catagctcct gtctcaacag cagcaatggc atccaagaca gcgtaagatg cttcatcagc
    23821 taactgacca aaaatcgtgt gacggcggtc taggtgaggt gtcccacctt gattggcata
    23881 gatttctgca atcggttctg gccaaccacc acgagtaatt tctttcttag aataaggtag
    23941 gtgttggttt tgcacgataa agaactggct gccgttggta tttggaccag catttgccat
    24001 ggaaagagca ccacggatat tgtaaagctc ttctgagaat tcatcctcaa aagattcgcc
    24061 gtagattgac tcgccaccca taccagttcc agttgggtct ccaccttgga tcataaagtc
    24121 cttgataata cggtggaaaa tgacaccatc atagtagcca tcttttgaaa gagatacaaa
    24181 gttagccact gttttaggag catgttcagg gaaaagcttg atacgtaagt ctccgtgatt
    24241 ggtcttaata gtcgcaagag gaccttctac tgtttcaatg tctacttgtg gaaaatgcaa
    24301 ttctttttct accataccaa atacttctaa ggcatcaaaa atgccatctt cttctaatgt
    24361 ttttgtaata taatctgctt tttctttgat tttatcatga gaaattccca tggcaacgct
    24421 gattccagca taatcaaaga gttccaagtc gttgagacca tctccaaaaa ccatgacctt
    24481 ctctggtttc aagccaaggt gttccacaac cttttccacc cccgtcgctt tggagcctga
    24541 aatcggcaca atatcagacg aatgttgatg ccaacgaacc atgcgaagtt tgtctgagag
    24601 actgtcaggc aagtgcaagt catctccctt atcttcaaaa gtccacatct gatagatatc
    24661 ttctttttca tggaaatcgg gatctacatc taagtcggga taaattggat tgatagcttc
    24721 actcatcata tcggtgcgaa tcgacaactt ggcatcatga ctcccaacca agccatactc
    24781 aattccttct tgcttagccc aagagatata ctcctcaaca tctgactttt caatctgatg
    24841 ctgataaatg acctgacctt ttttatcttc gatataagcc ccattcaaag ttacaaaaaa
    24901 gtcaggcttg agatcacgaa tctctggaac aacaccaaaa atgccacgtc cagaggcgat
    24961 tcctgttaaa attccttttt cacgcaactg tttaaaaaca gtgggaattg tagttggaat
    25021 aaaccctgtc tttgaattcc gcaatgtatc atcaatatca aaaaagacaa tcttgatctt
    25081 ctttgccttg tatcttaatt tcgcgtccat ctcactacct ctttcaatct aactctttcc
    25141 attatatcat aaagtaggca aatcccctat tttcaaaaag tttatcattt ttattttaat
    25201 ttcttggatg agaaaagaga catatttatg aaaaagctcc atcgtgcttt taatgtgttc
    25261 tcttgttttc aaactcgtaa aaagggagcc actgagccta actcgctctc tcatttcaaa
    25321 gcttgtgaaa aaagacccgt tggggtctta attcgctttc ttgttttcaa gctcatgaaa
    25381 aagagaccca accgggtctt ttctttaatc ttcgtttacg aaaggcatca aagccattac
    25441 gcgagcgcgt ttgatagctg ttgttacttt acgttggttt ttagctgaag ttcctgttac
    25501 acgacgagga aggattttcc cacgttctga aacgaaacgg ctaagaagct cagtatcttt
    25561 gtaatcaaca tattcaattt tgtttgctgc gatgtaatca acttttttac ggcgtttgaa
    25621 tccgccacga cgttgttgag ccatgttttt tctcctttat aagtttagtt gtccattaga
    25681 atggtaaatc atcatctgaa atatccaatg ggtttgttgc tccaaatgga ttttcattac
    25741 gtgaaaagtc tggtactgaa tttgtaggtg ctgaatagtt tgcagttggt gcagagtaag
    25801 ctccacctgt gtgaccctca cgcacactac ggctttccaa catttggaaa ttctcagcca
    25861 cgacctctgt cacgtagaca cgttgtcctt gctggttatc gtaactacga gtctggatac
    25921 gacctgtcac cccgataagt gagccttttt tagcccagtt agcaagattt tcagcctgtt
    25981 ggcgccacat aacgacattg ataaaatcag cctcacgttc accattttga ctcttaaatg
    26041 tacggtttac tgcaagagta aaagtcgcaa ctgctacatt tgatggggta taacgcaact
    26101 cagcgtcacg tgtcatacgc cctacaagta caacattgtt aatcatagtt taccttctta
    26161 cgcgtcaatt ttgacgatca tgtgacgaag aatgtcagcg ttgatttttg aaagacggtc
    26221 aaactcttta agagctgcat cgtcatttgc ttcaacgtta acgatgtggt aaagtccttc
    26281 acggaaatct tggatttcgt atgcaagacg acgtttttcc caagtttttg attcaacaac
    26341 agttgcaccg ttgtcagtca aaatagagtc aaaacgtgct accaaagcgt ttttagcttc
    26401 ttcttcaatg tttggacgaa tgatataaag aatttcgtat ttagccattg atatgttcct
    26461 ccttttggtc taatgacccc aagactttgc aaggggtaag tgaggttcgc tcacaacaaa
    26521 ctattatact agaaaaaatt tttttacgca agtaaaaaca ctagaattcg aaaaaacgcc
    26581 acatgggcgt tttcctgttc ttatggtttg atacggtgca acatacgtgg gaatggaata
    26641 gcttcacgga tatgttttgt tcctgctgcg aaggttacca tacgttcgat accgatacca
    26701 aatcctccgt gtggaactgt accgtattta cgaaggtcaa ggtagaattc atattctgta
    26761 cgatccatgc caagttcatc catcttagcg acaagggcat cgtaatcttc ctcacgcata
    26821 gacccaccga taatttctcc atagccttct ggagcaagca agtctgcaca aagcacgcgc
    26881 tctggatttc caggaactgg tttcatgtag aaggccttga tggctgctgg atagttcatg
    26941 acaaatgttg gcacaccaaa gtggtttgaa atccaagttt cgtgtggtga cccaaagtca
    27001 tcaccatgct caagatgctc gtagtcagca tcttcatcat tttcatgctc ttgcaagagg
    27061 tcaatggctt gatcgtaagt gatacgtttg aatggctctg caatgtagcg tttcaagagt
    27121 tctgtatcac gttccaaggt ttccaaggct tgaggcgcgc ggtcaagaac accttgtaga
    27181 agagctttca cataagcttc ttgcaagtca agcgactcat catgtgtcaa gtatgagtac
    27241 tcagcatcca tcatccagaa ctcagtcaag tgacggcgtg tttttgattt ttcagcacgg
    27301 aaaactggac caaagtcaaa gacacgacca agagccatag cccctgcttc taggtaaagc
    27361 tgacctgatt ggctcaagta ggctggcgtt ccgaagtagt cagtttcaaa gagttctgta
    27421 gaatcttctg ccgcatttcc tgaaagaatt gggctgtcaa acttcataaa accattcttg
    27481 tcaaagaact cataagttgc atagataata gcgttacgga tttgcaacac agctacttgc
    27541 ttacgagagc gtagccacaa gtgacggtta tccatcaaaa agtctgttcc gtgttctttt
    27601 ggtgtgattg ggtagtcttg agattcaccg atcacttcga tgtctgtgat gtccaactca
    27661 tagccaaatt tagaacgttc gtcctctttg acaatacctg tcacataaac agacgtttct
    27721 tggctcaagc gtttgataac atcaaacttc tcaagtccca cttcttcacc aaatttttcg
    27781 acaaagtttg gtttaaaagc cacaccttga aagaaggctg ttccatcacg caattgtaag
    27841 aaagcgattt tcccttttcc tgatttgttg gcaacccaag cgccaatcgt cacttcctga
    27901 ccaacatagt cttttacatc aataatcgtt acacgttttg tcattatttt tccttttctt
    27961 ttttattctt tatggcaaac cacctctata ttgttcccat ccaggtcaat cataaaagca
    28021 gcataataaa tcggatgctc acttcgataa ccaggaaccc cattgtctcg cccacctgcc
    28081 tctaagccag cctcataaca agcctgaact tcttccttat tttctgctaa aaaagcaaaa
    28141 tgaacaggat cttgtgttcc ctgagtcagc caaaaatcac caccaggatg agggctgttc
    28201 ggggatagaa aactaattag agaactagtc ttaaaagcca atttatagtc caaaggagcg
    28261 agaaaactcc tataaaatcc ttctgaaatt tgtaaatcct ttaccttaat ctcaaaatga
    28321 tcaatcattc tcactaccca taaatgcttt caagcgttcg actgcttctt taagcgtgtc
    28381 taggtctgtc gcatagctga ggcggacatt ttctggtgct ccaaatccag ctcctgttac
    28441 caaggccact tcggcttctt ctaagatagc agttgtaaag tctgtcacat ccgtgtagcc
    28501 tttcatctcc atggcctttt tgacatttgg gaagagatag aaggcccctt gtggtttgac
    28561 cacttcaaat cctggcacct ctgcaaggag gggatagatg gtattaagac gttcctcaaa
    28621 ggcctgacgc atgctttcta cagtatcttg ctcacctgat agagcctcaa ctgctgcata
    28681 ttgggctact gctgacggat tcgaagttgt ttgacctgca atcttggaca tggcagcgat
    28741 aatgtctgct tctccaacgg cataaccaat ccgccaacca gtcatggcat aggttttaga
    28801 cacaccattg atgaccactg tttgcttgcg aatcgcttcc gataggctag aaatcggtgt
    28861 gaactcatga ccattataaa ccaagcggcc atagatatca tctgctagga tgagaatatc
    28921 attttctaca gcccagtttc caattgccaa gagttcctca cgggtgtaaa tcatacctgt
    28981 gggattagat ggcgaattca gcaccaaaac cttggtcttg tcagtgcgag ctgcttctaa
    29041 ctgctctacg gtcaccttaa agtgattgtc ttccttagca gaaacaaaga cgggaacgcc
    29101 ttctgccatc ttgacctgat ctccatagct aacccagtat ggggttggga tgatgacttc
    29161 atcacctgga ttgaccacag ccataaagaa ggtatagaga gaatatttgg ctcccgcagc
    29221 gactgtcact tgatttgacg ctacagaata gccgtaaaag cgctcaaagt agctattgac
    29281 caccgcctta agctctggca gacctgaggt tactgtataa aaagaagcac gcccatctcg
    29341 aatcgatgca atggcggcat cttggatatt tttgggagta gtgaaatctg gctcacccaa
    29401 ggttagagac aaaatatctc taccctcagc cttcagtgct ttggcacggg ctccagcagc
    29461 caaagtcaca ctttcttcca tttctaaaac acggttggat agtttcatag gccctcctta
    29521 ttgaccaatg ctcctgtttc aaaatctact agataaaaat cagatcctga cttaacttcc
    29581 cagattggct tgtcttgata acgaccaaag gttatcttgt caatctcgcc agctcccttt
    29641 tccttagaaa ccgtttctgc tttttcttgt gaaataccct gatttagctg ataaacgtaa
    29701 atcttatggt catctttacc aatcaggaca gcaagcgctt cttgctgttt attacgacca
    29761 agaacgctgt aataagattc caagccattg tataaatcaa cctgatcagc ctgctctaat
    29821 cctgcatact gctgagctaa tttttctcct tcacttttag ctgtttgata gggtttcatg
    29881 ctaagagaaa ccatatacag aaaggaagca ctgataacca caaacaaaat cgtcatccct
    29941 agaccatact gccacagtag attatttttt gctttgtttt gtcttttttt cactcgtcta
    30001 ttttaccatc tattaagctt tattacaagt gaatataaga atactcttcg aaaatctctt
    30061 caaaccatgt cagctttatc tgcaacctca aagctgtgct ttgagcaacc aattctattt
    30121 ctcccttcaa acaaaaccga ttttgaaagt gaaacagttc ttactttttc agtcacaaat
    30181 gactagagtt tccgaaattt tacaattttc aaataaactc tgagcaaatt tcttgcaagt
    30241 tcttttgttt tgttgtaata tattttataa caacgagaga gttctcgaaa ttttaagaaa
    30301 aaggagacac atcatgtcta aaaaagtatt atttatcgtc ggatcactac gtcaaggttc
    30361 tttcaaccac caaatggcgc tcgaagctga gaaagcactt gctggtaaag cggaagttag
    30421 ctaccttgat tattcagccc ttcctctctt cagccaagat ttggaagttc caacacatcc
    30481 agctgtagct gctgctcgtg aagcagttct cgttgcggat gctatctgga ttttctctcc
    30541 agtctacaac ttctctatcc ctggtacagt gaaaaacttg cttgactggc tatctcgtgc
    30601 ccttgacttg tctgatacac gtggcgtttc tgcccttcaa gacaagtttg tcacagtatc
    30661 atctgtagcc aatgcagggc acgatcaact tttcgctatc tacaaagacc tcttgccatt
    30721 tatccgtaca caaagcgttg gtgatttcac tgctgcacgt gttaatgact ctgcctgggc
    30781 agacggaaaa ttggttcttg aagaaacagt cctaaactca cttgaaaaac aagctcaaga
    30841 cttggtcgaa gcgattcagt aaaaaataga aaattttaga aagtctaaat caattatagc
    30901 cacctaatag ggtggctttt tatttctcaa tcaagatttt cagacaaaca tcagccatcc
    30961 aaaaaaaacc ttgagcgtta ccactcaagg tttttagcag tcttttccat cataggaaaa
    31021 tcattctaag aatattcttt cattcgagat ctatcactta tcatcttctt tcgagaaatt
    31081 agtgactgct tttggctgat taatcttctt ttcttcgtcc tacatatagg ctcaatagaa
    31141 taccgctacc taaaagaaca gagatataat cagattggct tccagtttca ggtaatttgt
    31201 ccccacggtt ttctttacct gtagttggtg ctactgggtt agcatttact ggagttgctg
    31261 gttgagccgg tgttggtact actggttgtt ctggaaccgc tggtactggt ttaaccggta
    31321 cttctggtgt tgaaacagct ggagttggtt gtactggcac ttctggtgtc ggtacatttg
    31381 gtgttggttg cgctggcaca ggtttaaccg gtacttctgg tgtcggcaca tttggtgttg
    31441 gaggaactgg tttgtcattt gatggtgtta ctggtttgtc ttggcatcca cattttccat
    31501 ctttaccgtc tttgccgtct cgaccatcac gtccatctct accatctttg ccatctttaa
    31561 taactgtttt tgtcacagtt ccatcagaat tgatgattgt gattgtatga ctaccatcac
    31621 cattatcttc aacagaaact tttggtgatt taccatcttt accgtcttta atcaccattt
    31681 ctgtcttact tccatctgga ttagtgattg taacaacgtg agttccatcg ttattatttt
    31741 taacagttac agttggtgaa acgctatctt tgccgtcttt accatctttg ataatagatt
    31801 tgtattcacg accatcagaa tctacgattg ttaatgtatg agttccatct ccgttatcaa
    31861 cgatgttcgc ttttggtgat ttaccatcaa agattgtagt tgtcgttcct ttaccatcag
    31921 cacctgtcac tttaatagtg tgtgaaccat cgttgttatc aataacttca agttttggtt
    31981 ctctaccatc acgaactgta gttgttgtag ttgttccgtc tgagttcaca actgtgatag
    32041 tgtgagttcc atctccatta tccacaacct tagcagttgg tgatttaccg tcacgaactg
    32101 tggtttcaga aacagtacca ttaccgtttt ccacacgaat agtgtaagta ccatcttggt
    32161 tatctctaac tgttgctact ggtgatttac catctttaat gattgtttca gtagttgttc
    32221 cgtcaccatt tagaacagag atcttatgag ttccattttg ttcatcagtt atagtcactt
    32281 ttggtgattt accatcacga actgtggttt cagttgtcac accttctgga tttgtaataa
    32341 cgattgtatg acttccgtct ccattatcac gaacagatgc ggttggagta cgtccgtcaa
    32401 caccgtcacg tccattctta acaacaaact cattcttagt tccgtctggg ttagtgattg
    32461 ttactgtgtg gcttccatct ggattttcag tagttgttgc tgttgcagct ttaccgtctt
    32521 taccatcttt gatgatagcg ttggtaacat tgccgtctcc atctgtaact ttaacagtat
    32581 gagttccatc gttgttatcc gttacttcaa ctttaggagt cttaccgtct ttaccgtttt
    32641 taacaacagt ttctttagtt gaaccatctg gatttgtcac cgtaattgtg tggcttccat
    32701 ctgggttttc tgttgtagtg atattcgcag ttttaccgtc tttaccatct ttaacaactg
    32761 ttgttgattc agaaccatct gggttttgaa ctgtgatagt gtgagttcca tctccattat
    32821 cttttgtagt aatagttgga gctgaaccat cacgtccatc tataccgtta taaatatctt
    32881 tactatttaa tactttatcg acaccttcgt cgtatacccc attgtcatta acatcatcat
    32941 aaactgtaac acggataccg ttacgcgttg gagttgcagc agcatctgta gcgcgtctaa
    33001 ttcttgaagg tgaagtaata tcttctactt tgattttcgg tgcacggcca tctttaccat
    33061 tcttaccatc ttttacagtg aaggttactg gatctttacc tggtgttgtg atagttacaa
    33121 cagtgctttg gccatcttgt ccacgttgag tagtgattgt aggagtttgt ccatcctgac
    33181 cgattgcacc agtttctccc ttagcaccat cttgaacaaa gactgtagtt ggttgtgaac
    33241 catcatggtt atcaacagtg atttctacac ctttatgccc tgcttcatct ttacgaacag
    33301 ctgtaactgt aggtgatttt ccatctactc catctctaat taccgcaagt ggtttttctg
    33361 gatttaatgg ttgtcctggg ttagctggat cttcttggta aactttagtt tctttaccat
    33421 ccttgacagt aagcaatgat ttaccatctc gtccgtccgc gcctttagca ccatctttaa
    33481 tcatttctga acctagaagt tcatcagttc ctggtgtata tttgccgtca ttgttttcat
    33541 ctgtatagaa tgtcacaatt gtatgagatg gatttccgtt ttttccttgg acacgaagta
    33601 aatcaacttt tggagtttta ccgttcagac cattttctcc gtccttaaca tttactgtaa
    33661 ctggatcttt acctggaaca gtgaatgtaa tgtcagtact atggccatct ttacctggag
    33721 ttacagttac ttctggagtc ttaccatctt ctccgcgttc acctttgtca cccttagggc
    33781 ctttgatgtt gccctcttta tcccagttgc cgttattctt aacgaagacg tcgcctgttt
    33841 ctgtattaac gtatttatcg ccgtctttac cttgatgtgc ttctgggtta gctttgccgt
    33901 tcaacacgtc tttaccgtca cgtccatcac gacctactgc gccatccgca cctttagctc
    33961 cttgcgctcc agttaggccg cgttcgcctt ggtctccttt ttcgccttta gcaccatctg
    34021 cacctttgtc ccctttaggg cctttgatgt tgccttcttt atcccagttt ccattgtttt
    34081 taacatagac atcaccggtt tcggtattaa tatatttatc tccattttta ccttggtttg
    34141 gttgtggatt tacttttccg tttaatacat ctttaccatc acgacctgct tgtcctgtag
    34201 ctccatcacg gatcgtagtt gtcacatctg gacttcctgg attatgaacc gttaatgtat
    34261 gtgtgccgtt ttgcccctca gttattgtta ctgttggggt tttcccatca gcaccttttt
    34321 gtccatcctc tacaaatgtt gtttgtggac gacctcctac tggtgtaatt gttactaata
    34381 caccattttt accattttga acacttcgtg tcgctgtaat tgttggtgat tgtccattag
    34441 taccatttct tccatcagtg atagttgcta atggttggtt tggattaagt ggttgtcctg
    34501 gtctagccgg atcctctaca tagacttttg tttcagttcc atttttaaca gcaattagag
    34561 attttccatc tttacctgga gttcctggat taccttgttc tccttttggt ccttgtggtc
    34621 ccgctggtcc tctttgtcca tctgcgcctt ttggtccttg aactccatca cgaccttgaa
    34681 gaccttgttc acctttggca ccatctgcaa taataacttc ttttaatttc ttatcagaag
    34741 catctaattc attatttcca ttttgatcat aatagaagat aacagttgtt gtcttatctg
    34801 cttcatttcg ttgagtcgca actaatggac ttgcgccatc gaaaccatct cttacaacag
    34861 tacttgcaac attacctcta ccgtcgttaa tagtgatagt atgagtgccg tcatttttat
    34921 tatctttaac ggttacggtt ggagttacac cgtcacgacc tgcctgacct tgcgctccag
    34981 tcaggccgcg ttcgccttgg actccttttt cacctttagc accatcttta atagcaattt
    35041 tttgaacaag ttcgtctacg ccctcagtat aaacaccgtt tccatcgtta tcaagataga
    35101 aagtgaggtt atttgtatca ccgactttat tttgatcaac acgtggtttt actgtatcga
    35161 ttagatcttt agccacacca atttgcataa tacgtggctt aacttcttta ataactgaat
    35221 tactaattgg caatcctagg attgtttcgt tacccaattg atgaacttta tagatgattt
    35281 gacgttgtcc tttttcacct tctcgaacta atctagtttg accttctggt aacttactag
    35341 ttggaagata gacagtttgg aagtcaatgt cttctgaaac agtcttatta ttagtaccac
    35401 catttggatt tgcatccaag aggagtggtt tttctctgaa gtaatagtta gagttactat
    35461 atggatcatt actagttgca gatcctgatt ccccttgacc tacattaaaa gtaacaggtc
    35521 ttaatcgaac agacagatca ccattagggt tggctatagg taaactaaat ctaatagttt
    35581 gattactgtt tgtttcaaca gcagatgtta aactgtagct tgaatgagat acaaaatttg
    35641 caccctttct tatatcttta gtttgatctc ttaatctgtt tgttgaaata gttgcacgag
    35701 atggttctcc aaatccattc cctttgtcta agagaaagtt attattaacg aactcacctg
    35761 gaccagaata tgcaattgag aattctactc tatttccaac tctagttgtt gttacatcaa
    35821 aatgaatcgg gtttccacga cgtgaatttc cattaccacg gaaagtatga gaacctctgc
    35881 taatatcagt gaaaacagta ttatttccta gattagttac tggttggttc gttggagcag
    35941 gcgccgctct aaaaccacta taccctctat caacatcttc cttacggata gctggatttt
    36001 ggcgagcatc agaaaggctc aaacctggtt tcaagatttc attaatagat gctggagctg
    36061 aagttgtttc tactttggca gctgtgctag ttggcaaaac agcactatca acagctgaac
    36121 gatcagttgt tgcagctgga ttagtctctt ccttcgcttc aactatatta gctttcttcg
    36181 ctacaggagt ttctgataca gttcctttgt tagagttaga acgaagttct agaccaataa
    36241 tagatgactc tgctgctgat aattcagctt cagatgcatc tgctttccca agcaagcttt
    36301 gaccagcttg gatacttgtt tgcaagttag cagcacttgc ttctgtatat aacttgcttt
    36361 ctaacttttt agcacgcgaa agagccgtta acaattttga cttgtcaata gcatcttgat
    36421 gcgttacttc ctcttttttg tcttttgctt cttcaacttt ttctgctggt ttagtaactt
    36481 ctccagtttt aactggagtc gtttctactg gattagctac agttggagct gcataggttt
    36541 tctctttatt agacgcagaa acctctgctg gtttagtagc tgcttctccc ctaccaacag
    36601 aaatactatt ggtagtttct tgtttgtttt cttccgcttg tactgtttgg acactagctg
    36661 ctgcacctaa taccaaagcg gtaccgagca agacactaac tgcaccaaag taatacttac
    36721 gaatagaata acgttgttgc atgctatacc agtcaaatga ttttttatgt gaatgtttca
    36781 ttttaaactc ctagatagta ttttatagtt tacaatgtac aactatttga ggcattaatc
    36841 aatattttct gaacaattaa tatattctgt ttttgcactt gaaagccact tatattattc
    36901 taatttttcc gattactaac gacaattttc ttgctctaat ttttttatac tacttagaca
    36961 aaaaaagata taatgaatta tggaggaaat acccgtatga gaaacctttt atccacaaaa
    37021 gttcaaagac aattacgctt aatggaaacc ctaattcaga atcgtaattg gatgaaatta
    37081 catgaactag ccgaaaaatt aggatgcaca gaacgaatct taaaaagtga tttaaatgaa
    37141 ttacgtattg cctttccatc tattaacatc caatcttctg ttaacggaat catgatagat
    37201 ttagaagtta acactagtgt agaagatatt tatcaatatt ttcttgctaa ttctcaatct
    37261 tttcaattac tagagtatat gttcttcaat gaggggctac ctatttatcg aacaatagaa
    37321 aatctttact ttagtagtgc caacctctat cgactgggca gaaatattac aaaggttctt
    37381 tcatctcagt ttcaaattga attatctttt actccatccg agatacgtgg aaatgagatc
    37441 gatattcgtt acttttttgc ccaatacttt tctgagcgct attatttcct agattggcct
    37501 tttcctgacc ttcctgagga agatttaaca gagtttgctg acttcttcta taaaatcacg
    37561 aattatccaa tgcgattttc aatttataga atgtacaaac tgatgatagc tatcagtatc
    37621 cacagggtta aaaatggtca cttcattgac ctccccaacc acttttacaa agaatattat
    37681 cctcttttaa agagcattcc aaatttccag gagacacttg cttatttctc taaacatttt
    37741 gggcttgaaa tgactccaga tacaatcgct caaattttta tttcttttct tcagaatgat
    37801 attttcctag atcctcaaga attcttcaac tctttagaag ataacagtca agcaagatac
    37861 tcttaccaat tacttagtca aatattagaa cgcctatcaa aacaatataa gattactttt
    37921 actaaccacg atgagctgat ttggcattta cacaacactg ctttctttga aagacaggaa
    37981 atcttttcta ccccaatctt atttgaacaa aaagcattga cgattaaaaa atttgaagtt
    38041 tacttcccag actttatggg aagtgcgcgt caagaactga ctcaataccg tcaggcaatt
    38101 ggacagcatg accatcctga acagttggaa cacttgatgt acaccatctt aacccatgct
    38161 gaaaacctct ctactcagtt gttagaaaat cgaccaccta tcaaggtttt gattatcagt
    38221 aactttgacc atgctatatc ccttaccttt gttgatatgc tttcctatta ctgtaataac
    38281 cgctttacct tcgatatttg ggatgaatta aaaacaagtc ctgagatttt aaatcagaca
    38341 gattacgata tcatcgtgtc taatttctat attccaggga ttaccaagaa gtttatttgc
    38401 cgaaatcatc tgtcaatcat gaatttggtt aatcatctta acaccttatc aaatgagatt
    38461 catttgtcca atactttata agttatcaaa aaagtcagca taatcgtgct gacttttctc
    38521 ttatctgatt tctatacttc ctttacaaaa accaatccct gtggttgtga caaatcttct
    38581 cggtaaacaa atccagcaaa gttcaagcga cgtgcttccc ccacagtttg tacttgattt
    38641 tctattaaag ctgttagaaa ggccccgcgc gctttcttgg agatagttga gtgaatcttc
    38701 agctgaccgc ctctatcctc catgaatttg aaggtcacca tctttgctct gatttcctta
    38761 gaaaatacag tctcaaactc tgatgacaag agagagaaaa tcacttcttc cttcttcaga
    38821 gcttcatcat agactgcctt ccaatggctc ttcaaagtct taccagcgac ttttaatttc
    38881 atcaaaaaat ccaaacggtg aggagccatg ggtgacaagg ctggaacaac accgtacaaa
    38941 gccgaggtaa tgaagacatg attttcaaga taatcttgtt ccgcctcggt cagcttatct
    39001 ctcttaatgt tgcggtacat aagcccatca aaaagtttca aggctggata gtgttgagca
    39061 gtttgccttt tcaaagcttg gatattttga aattcttccg ccgctttctc agctgatacc
    39121 ttgtagaaac tctccaattg actggcagaa tagagagcca aggcatcaag cacggtctga
    39181 ctttctggtt ttaaaggaat tgcctcgata cttggcaagt ctgtgttcat ttcttttgct
    39241 gttgggatta aaattttcat attgatagtg tagcatattt tttagccaca accaagaaat
    39301 tcatttgaaa aacctcggtt tgaccgaggt ttttctattt atttgggcca tcctaaccag
    39361 acagccgcga gaacaagggc tatgccagct aaacttgtta agatagataa gaatttatct
    39421 tttttctcct tgaactgaga aaaacctatc ttcaaagcga gcagtccgag tagcattgaa
    39481 gcaaccatca aataaaaacc catttctttg tcctccatta ggcttaattt accttattat
    39541 aacatatatt tcttaaataa acctttttta tactcaatga aaatcaaaga acaaactagg
    39601 aaactagccg caggctgctc aaaacactgt tttgaggttg cagatagagc tgacgtagtt
    39661 tgaagagatt ttcgaagagt attattgtac tttaaaggtc ttgagataat tgtcttttcc
    39721 tacttgacct tcgaaggttt taccattttc aaggtaagga aggtcatcgg atactgaagc
    39781 cttgaccttg tatatcttgc cctcaacttt aaagaagtag acagtgtctc ccttgataac
    39841 agctgatttg aggtctgcta ctactccctt gatgctttct gtcgttgcat tgtcaatttc
    39901 aaggtcgttt ttattggcat acttgctgag catctcttcc actgtagtag caacgataac
    39961 attttggtac tcgactgcgt ctaccagggc gtactctttg accaagccag cattgtcctt
    40021 taagcccatg atgtagagag gcttgtcatt gaggttgatg aggattggga aggttgcttt
    40081 gtaggatttc tcctgaacag caccttctgc tgattcacgg gctgattctt ctgtcgcaga
    40141 agccaagcta tacttagtga tttctcctgt tcgcatattt tcaaggatga aaccaagatt
    40201 actctcatcc gcattagccg acgtcacacc tgtgtagaga tagatgtcat taccgataga
    40261 caagtaatta tagcccttgg tagtctgggt cacgtttttc ttggaaatca tggcattcaa
    40321 gaaaccgtcc ttgtacttgc cgttgtagtt aatttgctca atggtttcct ctgctggata
    40381 gatcctgtcc acccattctg gaacatctga taagctgtat tccttggttt ctccatttgt
    40441 agcatccaag ataatgactg aagcaggacg aggaacagca agtccaaatt gcttttggta
    40501 aaccgttgct acatagaaag gattgccctc atcgtccacc tcaaaagatg gagttttaaa
    40561 gattttggtc gggtacttca agcgcaggtg acgtttgaca tcacggttaa aatactccga
    40621 gtctgaatac ttgattggtg tcttcaagtc caccaaatcc gcatttccag ttaccatgtc
    40681 caccttgata tactcaccga ttcccttggc ttgattgtta aaccatttga tagggtctgc
    40741 atattctagt ggtgtgaccc gataaggttt cccatcaatt gtcaattggg tataggtatc
    40801 tgccgctacg tattgcgaca ccttatcggt tagggaaccc aagtagcggt ctccaatttt
    40861 ttcagcagta cttctatcta ggataggaac cttactggtg tcactcttag gaaattcagt
    40921 aaagtctttt tccgtaaccg tgactacatt ggcataattt ttagcctgaa acatgctaga
    40981 agttaccaag gaaaccaaag ctgccaagag aagaattcct ccaatcgaag ctaacagaat
    41041 tttccctaac cgattgattt tgaaaccctc aagatttaag gcagcttccg ccttaccgtg
    41101 gcgcacatga accgttttga caaggtttat ccccttgcca aagccaaata agattgccac
    41161 aactaacaaa tgcccacaga ggaagaagag aaattcccag ctggtcaggt taaggggcgg
    41221 taaaaagata taccaggtcg ttgcgataaa aataagttca aagactattc gtttcatttg
    41281 ctttcctcct aaatgattcg tccttccaag tgatccaact catgctggca aatctgagct
    41341 gggaagcctg tcaaggtaat ggtctgttcc tgccacttgc tgtcacgata ggcaagcctt
    41401 gtggtttcat aacgcttagt tgatctcaca cctaccaagg acaaacagcc ttcttctgcc
    41461 tcataggatc cttcaaagga cagaagcact gggttaaaca tgaccacggg aaccaagcca
    41521 agattaaaga taatcacgcg cttctgcacc ccaatcatat tggcagctag accaacacag
    41581 gtatcacgat ttgctaagag tgtatcctgc aaatctctgg caagataaag gtcctcctga
    41641 cttgccggct gagacacttg agataaaaat aagatatcct tcacaatttt cttttccact
    41701 tcctcacact cctcttgctt tttactatat tatagcaatt atagcacaaa agccgataac
    41761 tgcaaaccct ttccctcaac tgtagcaaaa agccatccga agatgacttt tttgctattt
    41821 aatttctgta taagttactt ccaagccacg cttaacagct ggacgattgg caattttttc
    41881 tgcccatttt actagatttt gataacttga ggcatccaag aattttgcag aaccttggta
    41941 aagatttcct tgaactaact gtccatacca agaccagata gcaatatctg caatcgtata
    42001 gtcattgcct gcaatataag gtttctgagc caattcctta tccaataaat ccaactggcg
    42061 tttcacttcc attgtaaaac ggttaatagg atattccaat ttttcaggag cataattgaa
    42121 gaaatgtcca aatcccccac ctagaaaagg tgctgcacct gcttgccaga atagccaatt
    42181 caaaacttct accttttcca caggattact tggtaaaaag gctccaaatt tctcagcaag
    42241 gtaaagaaga atatgagcag actcaaagac tcttacatct tcagtacctg actggtccaa
    42301 taaggctgga atcttggaat ttggattgag cttcacaaag tctgatccga attgatcccc
    42361 atccatgata gcaatcttat acaagtcgta agccgcttcc ttaaaaccag cttctagtaa
    42421 ttcttccaat aagatagtaa ccttcacacc atttggtgtt cccagtgaat aaagctgaaa
    42481 agcttgttct ccttttggca agttttgttc gaaacgggca cctgctgttg gtctgtttag
    42541 ccccgtaaaa gctccttgat tactagcttc atcctgccat acggtcggta attgatatgc
    42601 tgacatccga aacctccctt aaatcgcatt cttgtcaaaa ccgagtttgc gttgaataaa
    42661 cttaacgatt tcgacgatga taatcattga gaagcttcca gccataacaa ttccccattg
    42721 tgacaagtct agtttggtta cgtggaagat tccttcaagc ggttctacaa cgattgttgc
    42781 catgagaagg ataaaggata ccaagatgga ccagttaaag gtcttagact tgaatgggcc
    42841 aactgtcaag atggattggt agacagactt gacattgtag gcatggaaga gctgaatcaa
    42901 accaagggtt gcaaaggcca tcgttagggc atctgcatga atagcatgat tgtcacccac
    42961 atgaactggg taagcaatcg caaggccata aacactcata acaagagctg cttggagtac
    43021 accttgataa atgatagaac tcaaaacacc acctgagaag aagcttgcct tgcgtccacg
    43081 tggtttatga ttcatgacgc caggttctgc aggttcaaca ccaagagcga tagctgggaa
    43141 ggtatccgtt accaagttga tccacaaaag atgaaccggc tgtaagacat cccaaccaaa
    43201 caaggttgat aggaagatgg ttaatacttc agcagtatta gcagaaagta ggtactgaat
    43261 agtcttttga atgtttgaga agaccttacg tccttcttcc actgcgacga taatagtcgc
    43321 aaagttatca tctgcaagaa tcatatcaga agccccctta gaaacctctg taccagtgat
    43381 tcccataccg ataccgatat cggctgtttt cagagctggc gcgtcattga caccgtcacc
    43441 tgtcatggca acgactttac cttgtttttg ccaagccttg acgatacgaa ccttgtgttc
    43501 tggagacaca cgggcataaa cagagtattg accaacgact ttttcaaatt cttcatctga
    43561 cagttcattg agttcagcac cagttaaaac gtgaccttct gtatcgtttg cgtcaatgat
    43621 tcccaaacgt ttggcaatgg cttccgctgt gtcttggtgg tcacctgtaa tcataattgg
    43681 acggattccc gcttccttag ccacacgaac agcctcagcg gcttcaggac gttcagggtc
    43741 aatcatccca atcaaaccag taaaaattaa atcattttca agctcttcag aagtgagatt
    43801 ttctggaata ctatcgataa tcttataagc acctgcaagg acacgcaagg cttgatgagc
    43861 catttcagaa ttgtttgtat gaatgagatt tgtaaccttc tcatcaatcg gagcaatatc
    43921 cccagcctta tcacgaagaa gacaacgttt taagagttgg tctggcgcac ccttgactgc
    43981 tacaaggaaa cgaccatctg gcaatgggtg aactgttgac atgagcttac ggtcagagtc
    44041 aaatggcaat tcagctacac gaggatattt ctctaagaaa cctttgacat catagccctt
    44101 gtccaaggca tattggataa aggctgtttc ggttgggtca ccaatcaagt taccttccac
    44161 atcgattttc gtatcattgg ccaagacaac tgaacgaagt agtggcattt caagacctag
    44221 ttcaatatca tcagctgagt catgtagaac cgcatcgtag aagacttttt cgactgtcat
    44281 cttgttcata gtcagcgtac cagtcttatc agaagcgatg atttcagttg aaccaagtgt
    44341 ttcaactgct ggcaacttac gaacgatgga atgtcgtttg gccaaaactt gagtaccaag
    44401 agaaagaacg atggtaacga tagcaggaag tccttctgga atggctgcaa cggcaagggc
    44461 aacagaagtc aacaactcac caagtggatt tttcccttga atgaagacac ccactacaaa
    44521 agtaacaagg gcaatgacca agatagcata ggtcaagacc ttagaaaggt tgttcaaatt
    44581 ttgtttgagt ggtgtatcag tctcatccgc atcttgaagc ataccagcaa tatgaccaac
    44641 ttcagtgtac atacctgtat tgacaacaac acccatccca cgaccatagg ttacgtttga
    44701 gttttggaag gccatgttga cacggtcacc aataccagca tctgtcgcaa gctcgactga
    44761 caagtctttt tcgactggta cagattcacc tgtcaaggct gcttcttcaa ttttaagaga
    44821 gttggcttct atcaaacgta ggtccgctgg taccacgtca cctgcttcaa gggcaacgat
    44881 atcgcctggt accaattctt tagagtcaat ctctgccatg tgtccatcac gaagaacgcg
    44941 ggcaactgga ctagacatgg atttgagggc ttcaatagct tcttcagctt ttccttcttg
    45001 gtaaacacca aaggcagcgt tgatgataac cacagctagg atgataatgg catctgcgat
    45061 atcttcccca ccagaagtca cgactgacaa gattgctgcc gcaactagga tgataatcat
    45121 caaatcctta aattgctcga tgaatttgac caagattgat cgtttctcgc cttcttcgag
    45181 ttcattgtgc ccaaattcgg caaggcgctt ttccgcctca cttgatgaca aaccttgctc
    45241 ggtcgcatcc acagcctgca agacctcttc agggctctga gtataaaacg cttggcgttt
    45301 ttgttctttt gacatgtgtc tcctccttga cattgtgtgc aaaacagact ctctttctgt
    45361 catatctttt cacgacaaac aaaaagagac ctgttaatca taacaagtct cgctgtttaa
    45421 gatagggccg gaaagcatac ttttcagcat aaaattcgga atgacgacac tatcacaggt
    45481 ttctgccagc tactcccttg agtagtacca ttataccaaa ttttggggag ttttcaaaga
    45541 gtaaaaactg ccttatttga atttttcctt gaaaaccagt ataatgatag aatgctatgt
    45601 gactagaaag gaagttgaat gaagcaatct atctcaaatc tcaagttagc tgagcgtgga
    45661 gccattatca gtatttcgac ctatttgatc ttgtctgcag ccaaattagc agctggtcat
    45721 ctccttcatt catccagttt ggtggccgat ggttttaata acgtatcgga catcattgga
    45781 aatgtggccc tcttaatcgg gattcggatg gcgcgccagc ctgcagaccg tgaccaccgt
    45841 tttggtcatt ggaagattga agatttggca agcttgatca cttctatcat catgttctat
    45901 gtcggtttcg atgttctaag agataccatt caaaagattc tcagtcggga agaaacggtc
    45961 attgatcctc ttggtgcaac tctaggaatc atttctgcag cgattatgtt tgtggtctat
    46021 ctctacaata ctcgcctcag taagaaatcc aactccaaag cgctgaaggc agctgctaag
    46081 gacaatcttt ctgacgctgt tacctcactt ggaaccgcca ttgccatcct agctagtagt
    46141 ttcaattatc cgattgtgga taaactggtt gctatcatca tcactttctt tatcttgaag
    46201 actgcctatg atatcttcat cgagtcttcc tttagtcttt cagatggctt tgacgaccgc
    46261 ctgctcgagg actaccaaaa ggctatcatg gaaattccca aaatcagcaa ggtcaaatcg
    46321 caaagaggtc gcacctacgg tagcaacatc tacctggata ttacactaga gatgaatcct
    46381 gacttgtctg tttttgaaag ccatgaaatc gcggatcagg tcgagtctat gctggagaat
    46441 cgttttggcg tctttgatac cgatgtccat atcgaaccag cacctatccc tgaggatgaa
    46501 attttagaca atgtctataa aaaattgctt atgcgtgaac aattgatcga ccaaggaaac
    46561 caactagaag aactcttgac tgatgatttt gtctatattc gccaagatgg agagcagatg
    46621 gataaagagg cttataagac caaaaaagag ttaaattctg ctatcaagga cattcaaatt
    46681 acttccatca gtcaaaaaac caaactcatc tgctatgagt tagatggtat catccatacc
    46741 agtatctggc gtcgccacga aacctggcaa aatatctttc atcaagaaac caaaaaagaa
    46801 tagagaaatc ctttcatgag acgggatttt tctattcgtt tatactcaat aaaaatcaaa
    46861 gtgcaaatta ggaagccggt cacaggctgt acttgagtcg gcaatgtgaa gccgacatag
    46921 tttgcacttt gattttcgaa tagtcttaac tatcaaattc actgagatac tcatagcgtt
    46981 cgtatttttc aaggagtgct tcatttttct catccaattc tttttggaga gtagccagct
    47041 taccaaagtc agagccgtta gcctgcattt cctcttcaat agcagcgata cgtttttcca
    47101 aggtttcaat atcaccttca atacttgccc actcctgctt ttcttggtag gtcatgcgtt
    47161 tcttgtcttc tcgaaccttg accacttttt ccttttcggc cttttgcact tgattggcca
    47221 tatctgtttc aaaagctttt tcatcaagat agtcggtgta atgaccaaag aaaggacgaa
    47281 tcttgccatc ctcaaaagcg agaatcttgg tcgctacctt atccaagaaa tagcggtcgt
    47341 gactgactgt taaaacggga cctgcaaaac cttgcaagaa attctctaag actgtcaaag
    47401 ttgcaatatc taggtcattg gttggctcgt ctaaaagaag aacatttggt ttttccaaaa
    47461 gcagtttgag gagataaaga cgttttttct caccccctga caatttctca atcaaagtcc
    47521 catgcgtcga acgtgggaag aggaattgct ccagcaactc agcgatggaa gtcgtagaac
    47581 caccactggt cttgacctcc tctgccactt cctgcaggta attgatcaca cgcttgcttt
    47641 catccaaacc ctcaatttgt tgagagaaat aggcgatgcg aacagtttcc ccaatcacaa
    47701 cttgtcctgc tgtcggctca agacttcctg caatcaggtt aagtagggtt gattttccaa
    47761 caccattgtc cccaacaatt ccaatacggt ctttagcctg aactaagaga ttaaaatttt
    47821 gcaaaatggg cttattttca taggcaaagg aaacatcctg aaactcgatg actttcttcc
    47881 caatccgact ggtttcaaag ttcatagtca agtctgtctc agcactactg cctgaaactt
    47941 cctttttcag atcatggaaa cgattgatac gagcttgttg cttggtcgca cgtgcctgcg
    48001 gttgtctgcg catccaggcc agttcttgtt tgtagagttg ttctttttta tgaagtagag
    48061 ctgcatcgcg ctcgtcctgt tctgccttaa ggcgaacata gtcctggtaa ttgccctgat
    48121 attctgtcaa gcctgctcgc tccaactcga aaatccgtgt tgacaaagcg tctaagaaat
    48181 aacgatcgtg agtgataaaa aggacggtct tcttagaatt tttcaaaaag agggtcagcc
    48241 actcaataat cgcaatatcc agatggttgg tcggctcatc caaaagcaaa aggtcgtggt
    48301 tgccaagtaa gacttgtgcc aactgtaccc gtcttctcag accacctgac aattccccaa
    48361 caggagtaga taagtcttga atgcccaatt tgctaagaac ggtcttgacc tgactctcga
    48421 tttcccaagc ttggagagag tccatctctg ccatgacacg ttccaaacgc gcctgcttgt
    48481 cctcactata gtcgagcata atcaattcat actcacgaat gagctggatt tccttgagtt
    48541 cactagatag aaccgtatcc aaaactgtct ttctatcatc aaaatcagga tcctgagtca
    48601 agtaaccaat ctggtaatca tttttagctg aaaaaggact gacatcccca tcaaatccag
    48661 aaacaccaga aaggacgtcc aaaagggtgg tcttgccagt cccattgaca ccgattaaac
    48721 caattctgtc taagtcatgg ataataaagg aaatatccct aaaaacggtc ttgtcaccaa
    48781 cggatttact tagtttttca acgataaaat cactcatttt ttctccctca ggtaagcatg
    48841 gatggcttca cgattattct ccaattctcc atcgacaatg gcaaactcaa tctctgttaa
    48901 aatctctccc aagtctgggc ctggctgata gccatattcc ttgatcaaaa tatcgccatt
    48961 aatctgaatc tctttcttgt catggatagt caagctttgg tatttttctg tgatggcttg
    49021 tgggttgact tcttttcctt gagcttgacg aagattttca gcctgtaaaa gcaaatctat
    49081 gtcaaagcga taacaatctc gcttgctcaa ttctccattt tcacgcagag ccaaaataat
    49141 cagcaaatcc tgaacttgct tggcaaactg gcgtgaggtc ttccaagatt tcaaaaatga
    49201 ctgcgcattt tcaatctcca aagcccatag taaagccgcc caggcttgtt cagaggattc
    49261 aaaagtaaaa tcagtctcca aatcaaacag tctgttgagc ttgtcctggc tagatgccat
    49321 atcagggaga tagtcataag cttgactctc aatcatggaa gccaagcccc ttctccaaaa
    49381 tggagccagc aagagtttat caaactcgac gaaggtacgc tctacagaaa ttttctccaa
    49441 aagcggcgtc aaggtcttca tagctttaaa tgtttctggc tcaagtgcaa aaccaagact
    49501 agcctgaaaa cggaaaccac gcataatccg taaagcatct tcgttgaaac gctcactagc
    49561 cactccaact gctcgcaaga cttgcttttc caaatcttct aaaccatgga acaagtcaac
    49621 gatttctcct gtctcatcca aggcaaaggc gttgactgtg aaatcacggc gtttgaggtc
    49681 ttcttctagc gatcgtacaa aggaaaccgc actgggtctg cgatagtcca catagacatc
    49741 ctctgtccga aaggttgtta cctcatactc ctcatcccca tctaagacca agacggttcc
    49801 atgctcgatt ccgatatcgg ctgttcgcgg aaaaatctgc ttggtctctt ctggataaga
    49861 agacgtcgca atatccacat cgtggatagg gttatggaga agggcatctc gaacagagcc
    49921 cccaacaaaa taagcctcaa agcctgcttc tttaattttt tctaatactg gtaaagcctt
    49981 ctgaaattca gaaggcattt gcgttaatct cataataagt gttctaatcc atagacaagc
    50041 tcatgacgct tgacaacttc tttaattccc aaattgactc ctgtcatgaa ggagatgcga
    50101 tcataggagt catgacggag ggtcaaccct tctccctgat tgccaaagat gacttcctga
    50161 tgagctacca agcctggcaa acgaactgag tggatgcgca taccatcaaa gtcagcacca
    50221 cgagcaccag caatcagctc ttcctcatct gctgcgcctt gctgaatgga ctctcgaacc
    50281 tctgccatca actcagctgt tttaatggct gttccactcg gagcatcctt tttcttgtca
    50341 tgatggagct caataatctc cacatttggg aaatatttgg cagcctgcgt cgcaaattgc
    50401 atgagtaaga cagcacccaa ggcaaagtta ggggcaatca ggccacccaa gtcttgggca
    50461 cgagaaaatt cttttagctc tgcaatttct tcactcgtga aaccagtcgt tccaactact
    50521 ggagcaaagc cattttcaag agcaaaacgt gtattttcgt aggcaacagc tggagtagta
    50581 aaatctaccc agacatccgc ttcaaaacca gctaaatcag ccttatcctt gaaaacagga
    50641 ataccctgcc attctgactc agactcaaaa ggatccaaaa ctgctaccaa gtccaagtct
    50701 ggatcagtca ataccatctg acaagcagcc tggcccatct ttcccttaaa accggcaata
    50761 attactcgaa tactcatctc tactcctgtc taagatacaa agtccgtaag aacacaaagt
    50821 gaaaatagga attccaatca agaagtgtct acttcttgga agaactatct ttttcacaca
    50881 gggttccagg cgtgttcaat tatcaagata caaaggacct tagctgcctc tgaaaaatag
    50941 ggaatggcac tgactttcca cgaaaggcaa gacaggcatc ttttttcaag aggcaggtag
    51001 tccgtgttca atttctaaga tacaaggcat cttaactagc ctagaagcgc caactaaatc
    51061 actggaatat aacccagagc aatacttcct gctcctaggt gcgttccaat gacactacca
    51121 aatgtagcaa gtgaaacatc cgaacccaag ccaaaatcaa gcaagtgctg acgcaattct
    51181 tcagcctttt caggagcatt cccatgaatg acaatgaccc ggtattgacc tgaagccgtt
    51241 gtttccttga taatttcaat taagcgcttg gtggccttct tttcagtacg aactttttcg
    51301 taaacttcaa tcacaccttg atcgttaaaa taaaggattg gcttaatgct aagcaaattg
    51361 cccaaaatgg cagccccatt tgaaaggcgt ccacctttta ccaaatgatc caagtcatct
    51421 accatgataa aggctgacgt acggctgatt tgaatggcta gcttatcctg aatgctggca
    51481 aaatcatcgc cctgatcacg ccaattaaag acgctttcaa ccatgatgcc taggggagca
    51541 cttgtaatca aagtgtctgg gaaagcaatg gttaagccct catagtcatc gaccatatac
    51601 tggatatttt ggtaaaaacc tgaaattcca gaagatagga aaagccccaa ggcatgtgta
    51661 tagccttgtt ctttgagcga agttaagatc tcatctaact tggcaatact tggttgactg
    51721 gtcttaggca attcagaagc ctgagccatt ttttggtaaa attcctcagc agacagattg
    51781 atgccttcga catattcctc accatcaata ttgacaggaa tatccaagac aaacaagtct
    51841 tctctttgca aggtctcttc actgagataa gcagaggaat ctgtgaaaac agctaatttc
    51901 atattagaac tccaaattaa ttcctggtaa gtctaatgca atttcagtca cttcgtaagt
    51961 caaacgattg agcatgttca aacatggacg agccaaggtt tccacctctt cttggttcaa
    52021 ttcacttggt tcattgacaa tacggccatc gatatggttt acttgtgaga ttgttccact
    52081 aatgacaaac ttatcaaata caatcataaa gctcaagatg acaatcaagg aagtcacttg
    52141 attttcttgg tcatgttgga gcaattggaa attcacatcc accttggttt caggagctcc
    52201 attttcattt tcccattcaa aattacgcgc atcaaaatga tactgactaa caaattcttg
    52261 ttcacgttta agattcatgt ctttctccat cggctacaat attataagct attgtaccat
    52321 aattttttat tttcatctag ttttctagga tttagtcaat cccaatttca gcacgaacta
    52381 catctgtgat agtatcaaca tagtagtcca cttcttctgt tgtaggcgct tctgccataa
    52441 cacgcaagag gggctctgtt ccacttggac gaacaaggat acggccgttc cccgccattt
    52501 cttcttccat cttctcgatg atggccttga tagctggcac ttccatggcc ttttccttca
    52561 tgacgttttc cactcggata ttaactaatt tttgtggata aatcgttact tctgccgcca
    52621 actctgataa gcttttacca gttttcttca taattttagt caattgaact gctgataatt
    52681 gaccatcacc tgtggtattg taatccatca agataacgtg accagactgt tcaccaccaa
    52741 ggttgtagcc tgattttctc atttcttcaa caacgtagcg gtcaccaact gcagtaactg
    52801 ccttgttaat accttcgcga ttcaaggcct tgtggaaacc aaggttagac ataacagttg
    52861 tcacaattgt attttgagcc aattgtcctt tttcagaaag gtattttccg atgatgtaca
    52921 taatcttgtc accatcgacg atgtcaccat tctcatcaac agcaatcaag cggtcactgt
    52981 ctccatcaaa ggccaaacca atagctgacc cactttcttt gaccacttct tgaagggctt
    53041 ctggatgtgt tgaaccaaca ttaaggttga tgttaagacc gtctggtgtt tccccgataa
    53101 ccgtcaattg ggcaccaagg tctgcaaaga tttgacgggc actggtagaa gctgctccat
    53161 tagctgtatc caaggcaacc ttcattccat caagaggagt tccagttgaa acaaggtatc
    53221 cttcatactt acgcaagcct tctggataat ctaccaaaat tcctaagcct tctgcacttg
    53281 gacgaggaag agtgtcttcc tcagcatcta gcaaggcttc aatttctgct tctttttcat
    53341 catctagttt gaagccatca ccgccaaaga acttgattcc gttatcaagg gctgggttgt
    53401 ggctagcaga aatcatgaca ccggcacttg ctccttcagt ttcaaccaag taagctactg
    53461 ctggtgttgc aaggacacca agtttgtata cgtgaatccc tactgaaagg agacctgcca
    53521 ccaaggccga ttccagcatt tcccctgaaa tacgtgtgtc acgtcctaca aagactttcg
    53581 gcgcttccgt ttcatgttga ctaagaacat agcctccaaa acgtcctagt ttaaaggcta
    53641 attctggtgt tagttctagg ttagcttctc cacggactcc atcagtccca aaatatttac
    53701 ccattgttat aaaatccttt tctattttta attcgttttt gaactagttg ctttcgttga
    53761 cgaagatgtc tccgatgaac tgcttgtact tgaatttgat gtgcttgaac ttggtgctac
    53821 tggttttgta gtcaccttca ttattgtatc aaacggagtg ataactgccg gtaagacaac
    53881 accattgcga tcgattgcct gcaaaggtac tgaaccactg taattacctg ttatacgttc
    53941 gctagttggc aagacagcga taatcttatc aattctatcc aatgtctctt ggtcactcgt
    54001 aatcgacact tctttatctg acaccatgac attttcaatt tgtacccgac tatcaatttg
    54061 actagggtca atctctggta caatctttac cttatccttc tgagccttct taccaatctt
    54121 gactgtaatt ttttgcggag tcgccacagc ggtcagccca ttgggtaaat cttcaatgct
    54181 caaaggaact tcaatcgttc caacaccggc atctgttagg tcagcagtaa ccttgaattt
    54241 acgtgtactt tcttgcattt cactagctag cgataggcga tttgcaccag tcaagaccac
    54301 tgatacttct gaagcaaaac cgctaataaa atacttatca ctattatagc gtatgtcaat
    54361 agggacattt gttactgtat tagtataggt ttccgttttt acctgcctag cactggtact
    54421 gttttgaaaa ttcgtcgccg tagcatagac aaataagaca caagcaaaaa agagtgagga
    54481 tatgatatat aaactatttt ttttcatgtt tccatcctcc tagccatcgt tctttaaaac
    54541 taagacccac ttcctctttt ggaagtaaga tttcacgtaa ttctgtttca aattcatcaa
    54601 gtgttaggtt gtgcttaaac cttccattat aggttatcga aattcctccc gtttcctctg
    54661 atacgacaaa agtcaaggca tctgagactt ctgataaacc gatagccgcc cggtgtctgg
    54721 tcccaaattc cttggaaata cctgtgtttt ttgtcaaggg cagataggca gacgtcacag
    54781 cgatacgttc ttctttgata atcaccgcac cgtcatgtag gggagtgttg ggaataaaaa
    54841 tgttaatgag aagttctgca gaaatcttag catccaaggg gattcctgtc gaaatatact
    54901 cctgcaaggt acgtacacgc tgaatagcaa ccaaggcccc gattttacga ggactcatgt
    54961 attcaacaga cttaacaaag gcacgaatca tctgttcctc agcactaata ggggcattgg
    55021 aaaagaaatc tgtcgctctt cccaaacgtt ccaaaccagt ccgaatctct ggagagaaga
    55081 taacaaccgc cgcaataacc ccataagtaa taatttgatt gattaaccaa gaaatcgtag
    55141 tcaaaccaat catatttgca aggatttgag ctaaaataaa taccaaaact ccacgtacca
    55201 aaatcataat cttggttcct gcaatagctt ttgtaaaatg gtataaaata taagcaacaa
    55261 tcaaaatatc aatcagattg atagctatcg tccatggact tgcaaacaaa ctggtccaat
    55321 attgcagatt ggataattgt tgaaaattca tccctgatat cctccctatc aaaacacttt
    55381 cgtcctatta taccattttc gggcattttt ttccctatcc tagtccattt tacattgaac
    55441 aaaaatatga taaaataaac tgactagaaa aaacaaagga gaaactatgt ctcaactcta
    55501 tgatattacc attgtgggtg gtggtcctgt cgggcttttt gcagcctttt atgcccacct
    55561 acgccaagcc aaggttcaaa tcatcgactc tcttccccag ctaggtggac aacctgctat
    55621 tctctaccct gaaaaggaaa tcctagacgt accaggcttc ccaaacctga ctggagaaga
    55681 gttgactaac cgcttgattg aacagctaaa tggatttgat acccctattc atctcaatga
    55741 aacggttctt gagattgaca aacaagaaga agaatttgcc atcacaactt ctaaaggaag
    55801 tcacctgact aaaacagtta tcatcgctat gggtggcggt gccttcaaac cacgtccgct
    55861 ggaacttgaa ggggttgagg gctatgaaaa tatccactac cacgtttcta acattcagca
    55921 atacgctggt aagaaagtga cgattcttgg tgggggagac tcggctgtgg attgggcttt
    55981 ggcttttgaa aaaatcgcac caactaccct tgttcaccgc agagataatt tccgtgcctt
    56041 ggaacacagt gttcaagcct tgcaagaatc atctgtaacc atcaagacac cattcgcccc
    56101 tagccaactc cttggaaatg gaaaaacact tgataaactt gaaatcacaa aagtcaaatc
    56161 tgatgaaact gaaaccattg atctagacca cctctttgtc aactatggtt tcaaatcttc
    56221 tgtcggtaac cttaaaaact gggggctcga cctcaaccgt cacaagatta tcgtcaacag
    56281 caaacaggaa tccagccaag caggtatcta tgctatcggt gactgctgct actatgacgg
    56341 aaaaattgat ctgattgcga caggcctcgg agaagctcca actgctgtca acaacgctat
    56401 caactacatt gaccctgaac aaaaagtaca accaaaacac tctaccagtt tataaaaaag
    56461 aaccacgagt cacataggat tcgtggtttt ataattcatc cgctatctta ttgatttttc
    56521 tgagtctgtg attgacacca cttttggtca gaggggtgct gaggctatct gccaactgtt
    56581 ggatagagta gtctgggtgc tgaatcctca gttgcgccac ttcctgcaaa tctactggca
    56641 aattttctaa gcccatgata tcttttattt tactgatatt gttaatggtc ttcatgctgg
    56701 cagaaactgt ccgagcgata ttagctgtct cggcattatt agcccgattg aggtcgttac
    56761 gggtttctcg caaaatctta acccgctcaa aatcatcacg tgcctgcatg gctccgatta
    56821 ctatcaagaa gtccataatg tcttctgctc gctggagata ggtcacagcc cccttcttgc
    56881 gctcaagcac cttggcatcc agtaaaaact gttggagaag ggaggcaatt ccttgcgcgt
    56941 ggtccagata aacagaactg atttccaact ggtacttgcc tgactcaggg tcacgaatgc
    57001 tcccatttgc caagaaagcg ccacagagat aggcacgacc tgcttcctcg tccgataaaa
    57061 tcgcctcatc aatacctgtt tccaggccaa agaaagagtc tgccaagtgc aaatcactta
    57121 acaaatcctg caccttttca tctgtaaaaa cggtatagac gcgattcttg cgaagattgc
    57181 tccgttggtg gtgacgaatt tcagatttga tttcatagaa atggagaaag gactcataga
    57241 ggtgacgggc cagtttggca ttttctgtca caactgacaa agtcaagccc gaagtcgaga
    57301 gaccgatgct accagacatt ttgataatgg cagataattc atgccggctc agatggtgtt
    57361 ggcccaggat ttcttctttt actgctactg tgaaactcat tttttcacct gtataatgcg
    57421 catcaactcg tccacaatca aatctccatc gtggaaggca ccgccatttt ccagacgaag
    57481 gaagttagat gaaatcacgc gcgaaacttg cttacaaaga cctacaaaat cgtgttccac
    57541 ttgcactaag tattcatcaa aacggttgga attcatgtat tcctgaggca ctttttcaat
    57601 attcaccaag acagtgtcga taaaagggcg accaaggtga cgatgcaaga cttccacgtg
    57661 gtcgctatct gtaaagtgtt ccgtctcccc acgttgggtc atgatattgc agacataggc
    57721 aatttctgcc ttagtttcca aaagagcccg cccaatttcc ttaatcacga tattgggcaa
    57781 aatagaggta aagagggaac ctggccctag gacaatcatg tcactttcaa ggatggtctg
    57841 cactactcga cggctggcca gaggcgtatc atcgtttagg gcattggtca catagacatt
    57901 gtcaattatg cctcgatggt ctacaatatg actctctcca gccacttctg tcccatcctg
    57961 aaagactgca tgaagggtca aaggatggtc actggaagga taaattttcc ctgttgtatg
    58021 gaaaaatttg ctcaataact gcatggcatt ataggttgaa ccctgcattt ctgacaagcc
    58081 agcaatgatg agatttccca atggatggcc agcaaaggct ccggcatcct cagagaaccg
    58141 atactgaaag accttctcat aaaacttagg catatccgac atggccacaa ggacattacg
    58201 aagatcacct ggcggtgtca actgttgcat attttttcgg agttcacctg aagaaccacc
    58261 atcatctgcc accgtcacga tagctgcgat ttccacatct ttttcccgca gactttttag
    58321 aatgacggga attccagtcc ctccaccaat caccgttatc tttggttttc tcatgaacgg
    58381 tttaccgttt cctttctgcg gtctttgtcg cgatgccctt cattaacaga ccaattcttg
    58441 gataagtcct gcgccaagcg tttagcaaat gccacactac ggtgttgtcc acccgtacat
    58501 cccatggcaa tggtcaaaac ggacttacct tccttttggt aacttggcag aatcggctca
    58561 atcaaggcca ataaatgttg ataaaagtct tctgactcag gatggttcat gacataatca
    58621 taaacaggtt catccacacc cgtttggttt ctcagttctg gtagataata gggatttggc
    58681 aagaaacgga catcaaagac caagtccgca tcaatcggga ttccatactt aaatccgaaa
    58741 gacatgactt cgatacggaa agactgggct tgttcttggt ctgaaaactg ctctgcaagg
    58801 gttttgcgca gctcacgtgg agtgagttca gtcgtatcca ccacattttg gctcatattt
    58861 ttcaaaggtg ccaagagttc acgttccaac ttgattccat ctaaaatacg accgtctgct
    58921 gctagtgggt gactccgtct ggtttccttg taacgagcga ccaattcctt atcagccgca
    58981 tccaaaaaga ggattttgaa atccaaacca tcttgatttt ccaactcatc caaaacagct
    59041 tgaatctctg aaaagaaaga acggctacgc atatccacta ccaaggccaa cttaggattg
    59101 tcttccttaa tttcaaccag ctgcaaaaac ttaggcaaga gagctggcgg catattatca
    59161 atggtgaaat aacctagatc ctcgaaggac tgaatggcta cagttttccc tgcgccactc
    59221 atccctgtca caatcaccaa gtgaaattgt ttctttgtca tctttttctc cttatatcaa
    59281 aagaagtttg gcaacaccaa acttcaacta gcttatccaa tctctgcgat gacttcaatt
    59341 tcgactttta catcacgagg aagacgagct acctccacag ctgaacgagc tgggaattcc
    59401 tctttgaagg ccgtttggta aacctcatta aaaggaacaa agtcgttcat atcgctcaag
    59461 aagcaagttg ttttgacaac atggtcaaag tctgttcctg cttctgccaa aatagcaccg
    59521 atgtttttca agacttgctc tgtctgttct tggatattct ctcctacaat ttccccagtt
    59581 tcaggggata ggggaacttg accgctagca aacaaaaggt tgccaacgat ttttccttga
    59641 acatagggtc cgatagcctt tggggcctta tctgtatgaa ttgtttttgc cattttcttt
    59701 tcctcacaat ttttctaaga ttgcatccca agcctcatcc atccctgcct tgctgacaga
    59761 tgaaaagagg atgaaatcgt cactcgggtc aaagtttaat ttctttttga ttgctgattc
    59821 atgcttgttc catttaccac gaggaatctt gtccgccttg gtcgccacaa tgatgactgg
    59881 aatctcataa tacttgagaa attcgtacat ctgcacatca tctgctgacg ggtcatgacg
    59941 aaggtcaact agactgacaa ccgcacggag attttcccga gtcgttaagt actcctcaat
    60001 catgcacccc cacttttcac gttccttttt agaaacacga gcatagccat aaccaggcac
    60061 atccacaaag cgcatcttgt catcaatgtt aaaaaagttc aggagctggg ttttaccagg
    60121 ttttcctgat gtacgggcga gattcttacg gttcaacata gtgttgataa agctggattt
    60181 accaacattt gaacgccctg ctagggcaat ctctggcagt tcatcctgcg gatagtggga
    60241 cttattagct gcactgagca agatttcagc attgtgtgta ttaagttcca tagtcacctc
    60301 taggctgttt ctaggatcgg tttatccgtt ccatcgacag tttctttagt gatgcgaacc
    60361 aatttcacat tttcctgact cggcacctca aacataacat ctagcatggt ttcttcgata
    60421 atggagcgaa gtccacgcgc ccctgtcttc cgttcgattg ctttattagc aatctcttga
    60481 agggcttcgt cgtcaaattc caactcaaca tcatcataag aaagcaaagt ttggtattgt
    60541 ttcaccaagg catttcttgg ctctttcaag atgcgaacca agtcatcaac ggtcaattgc
    60601 tcaagagccg caaaaacagg caagcgtcca atcaactcag ggataatacc aaatttttga
    60661 atgtcttcag cgatgatttc ttgcatatat gagctgtttt cgtcaatcgc cttattattt
    60721 tgaccaaatc cgatgacttt ttcacccaga cgttgtttga caatttcttc aataccatca
    60781 aaagcaccac ccacgatgaa gaggatattt tttgtatcca cttgaatcat ctcttgttgt
    60841 ggatgtttgc gtccaccttg aggcggcacg ctagcaacag ttccctcgat aatcttgaga
    60901 agggcttgtt gcaccccttc accagaaaca tcacgtgtga tagacacatt ctcactcttc
    60961 ttggcaatct tgtcaatttc atccacatag ataatgccac gctctgcacg ttcgatgtta
    61021 aagtcagcaa cctgcaagag tttgaggagg atattttcca cgtcctcacc cacataacca
    61081 gcctccgtca gagctgtcgc atccgcaata gcaaaaggca cattcaagct cttagccaag
    61141 gtctgggcaa ggaaagtttt ccctgaacca gttgggccaa tcatcaaaat gtttgacttc
    61201 tgcaaatcca catcttctga ctcttcgcgt gtatcgtgga aattgatgcg tttgtagtgg
    61261 ttataaaccg ccactgccaa ggcacgcttg gcacgatctt gaccaattac atagtggttc
    61321 aagatatgga ggagttcaat tggttttggc acctcagaca agtctgccaa gacttcctca
    61381 accaattctt ctcgaatgat ttcctgagct aactccacgc attcattaca aataaaagca
    61441 ttgttgccag caattatttt ttgtacttct tcttggtttt tgccacaaaa tgagcaataa
    61501 accatcatat cattttttct atttgtagac atgatttcct tccattctat actgtcattc
    61561 tatctaaaat aaggtcatgt aaaaagcatg aatactattg accagattgg taaaggcatt
    61621 taaccaaagg aggatagaaa gcccgtaacg ctttttacga aaagcctgtg ctcctgccag
    61681 aaagcagatg aaacacagaa aagccgtgaa tagaccaaat aaactccgtt ccattagact
    61741 tcctttctct tgcggtattg gatggtaaaa tcataaggat tcttctcatc tttggcgtaa
    61801 aatttgcttg aaactgtctc aaaaagagac aagtcaagct cttcagggaa ataggtatct
    61861 ccttccaccc gagcatgaat gtgagtgaca atcacttcat caaggtaagg ttcaaaagcc
    61921 tgaaaaattt gcttcccacc gataatgtag agattctttt cttgatcctg ataccagtca
    61981 agaacagact ggacgtcctg aaaagtagca accccatcta tcttttcttc cggattacgc
    62041 gtcaaaatca gggtttcccg ttttggaagc aagcgacgcc ccatcccatc aaaggtcaca
    62101 cgccccatca agatagcatg attcagagtt gtttctttaa agtgctgcaa ttctgctggc
    62161 aaatgccaag gcagacgatt ttccttacca atcacaccct cttcatcctg ggcccaaata
    62221 gctacgattt tcttagtcat gcttccatcc ttttcactga tagtactatt ttatcaaaaa
    62281 actcaaaaaa agactggttt ggaatagctt acaaaataga aaaaatctgt aagaaatttc
    62341 ctacagattt atctatgttt ccttatttct tacaaaccag gtgcttgtcc aagttcggct
    62401 gcaagcatcc aaattgtttt atctgtttca gttttagcgc ctgcaaagat accgtttgtt
    62461 acatcgtcac cttcttcatc agtgacatcc aaaccttttt ggaaaagttc tgacaagtaa
    62521 cggtagataa caagaacacg ttccaagctt tcttcaacat tacggtattc accagcttct
    62581 tcttcgattt cactattttg aaggaactct gtcaatgtag agaatgggct tccaccgagt
    62641 gtaatcaagc gttcgctgat ttcatccaat tgaccgtcaa gagcttccat gtactcatcc
    62701 atttttggat gccatacaag gaaaccacga ccatgcatat accagtgcac ttggtgcaaa
    62761 gcaacgtgag ctacatacaa atcagcaaca gcttggttca agacttcctt tgtttttgcc
    62821 aatgctactg gcgctgcttt tgtcaatgat gttacgtctt ttactgcttc ttttttcaat
    62881 tctaccattt tctttacctc attcattatt ttttgtaacc acttcttaat gattactacc
    62941 ttagtatact actaaggaaa accaatagca agacaaagaa cttatatgag aaaagttgca
    63001 atagactgat aatttaagaa ttctttagaa gacaaaaaag agggttggat gcttatagtc
    63061 aatggaaatc catcagcaaa ctaggaaact aaacccagct tgaccaaagc acaagttttg
    63121 aggtcgcagg tagaactgac caagctgatg tggtttgaag aaattcccca atactataat
    63181 agattgaaat aagatgaaac caagtcgatt aggaaaggta aatgaatttt taaaatgttt
    63241 taccaatcac aaggtctttg tctatattca atctactata taagcttcat ttccgagcaa
    63301 gatcttgaat ttactcgagg atgaactatc ataaaacaaa gaaaaagagg gtgctactat
    63361 cacatccctc tttcaaatca tcgcttaata ccaaacgctg acatctacgc gcccttggat
    63421 tcctttcatg tattcagaag aggtatattg ccaacctttt tctcctgaat aataagggtt
    63481 ttcccattct aaagcattcg tataggccgc tacccagttt acatgtttta aaatatctgg
    63541 gtgttttaaa cgcgtctgta ataaactacg atagctatag acatatacat tttgataacc
    63601 cgcctgcttc atcgtgtcca tgtacttgtt gatgatttta acccaagtgt ctgtatcact
    63661 tggagctctc ttgctcttat ttacatattc ccagttctca acatcataat agatagggta
    63721 agacaggttc atattgtatt tctttataag ttcaatggtc tgtttagcgt cattctcagc
    63781 atcggtctca ttttcagcat aggtatagag atagacacca taaggaattc ccagacggtt
    63841 taactcctta atgttatgcg ccaattcctt gtcttcttta ccgctataac ctagacgaac
    63901 aatgacacca tccacctcgt tctcatcaat aacctttttc caatcattga tacgaccatt
    63961 gtgctcacta atatcaatga ttttcttagc atgttcggtt cccacttgtt cttctctatt
    64021 attaaagaaa tagcgtttac catctctgtg aacccagccg acattcaaat ctttcttttc
    64081 tttgagctca ccagaggccg caaagatata gcgagcacca tccataatca cttcgtcagt
    64141 cgccatggca ccacttccag tcaaatagta gttgccttgc cactcattcc gagccatata
    64201 gccccacttc ttgaaatagt accatttgcc gcccactttt tgccactctt ggttagcata
    64261 agttccatcg gattttagat aaaaataagc agaataggct ggatcataga gccattcatt
    64321 ttgtatcata gctccttgac cattcaagaa ataacttcct tgccattggc ttttagccat
    64381 gtaaccccac ttcttgaagt agtaccactt atttccaatc aattgccatt cttgatgagc
    64441 ataagcccca tctgacttga gataaaacca actcttgtaa ttactgtcat agatccactc
    64501 gttttgggcc atatatccac ctgatttcag gtagtaattt ccatgccatt cattctgcga
    64561 gtaacgacca tctgaattaa tataaaacca agccttatag tagttgtcaa aaatccattc
    64621 actttgagct ttagaaccat catcctttac ataataatcc ccatcccagt gggcagcagc
    64681 attagtctgt acttctgcgc tcgtttgcgt tttattagaa gactgatttt gctctgaact
    64741 acttgaagct gtcgttgtat cctgcgaagt ttttgctact tcagtttcat ttgcagcgac
    64801 atgactagtt gccaagccta gtaaacagat acttgctaat ccaattttta ttttcaatct
    64861 ttcctctcct ataaaaaatg taacagacat ctgaatgctg ttccacctag cttttgctac
    64921 ttactgatta ttttacaaag tcaagcaaag ccaagaagct ttcagcttca agtgacgcac
    64981 cacctacaag ggcaccgtca acgtctgggc aagccatgta tgaagcaaca ttttcaggtt
    65041 taacagaacc accgtattga acacgaactt tgtctgcgac ttcttgacca aagtcagcag
    65101 ctacaacgtc acgaacaact ttacacattt tttgtgcatc atcttgtgaa gctgatttac
    65161 cagtaccgat agcccagatt ggctcataag cgataactga ggcagcaact tgttcagcag
    65221 tcaatccagc caatgcagca gatacttgag cacctacgaa ttcagcagct ttaccagctt
    65281 cgtaagtttc aagtgattca ccacaacaga tgattggaag cataccgttc gcaaagattg
    65341 cttttgcttt tttgttgata tcttcatcag tttcatggaa gtagtcacgg cgttctgagt
    65401 gaccgataac aacgtagtca gtaccgattt ctttcaaaac ttgtgggcta gtttcaccag
    65461 tgaaagcacc tgcattttca aagtagcagt tttgagcagc aactttaagg tttgagcctt
    65521 ttgcaacagc aagaacagtt gtcaaatcaa gagctggagc agcgatacct gcttcaacaa
    65581 gatctgatga aggaagtttt gatgcaactg cttcaacgaa tgctttagct tcttctggat
    65641 ttttgttcat tttccagtta ccagcgataa atggtttacg tgacatttca catacctctt
    65701 ttttcaattt attttctatt tattctatca caattataca cagcttgcaa atcttactcg
    65761 gatttcaagc ctacttgcat ggattaatcc ttccagagat ccatggcatt tctgaaatct
    65821 gcagatacct gtgtcaactg aggattgctt gcttctcttt ctgctctctt ggcctcaatt
    65881 tgagccacac tcttgattcc ttcatggcgc cagtttctca aaatcgcctg aatgtacttc
    65941 cagtttggtt ttccattcaa aacagcttca cgaagagcct ccttaatcaa gtcagcactg
    66001 gttccatctt cctttagtgt cttgatcaaa tcctcaatct caaaaggcgt caacaatcgt
    66061 cccaactcct gctggaaggt ttccaccaaa tccttgagct ggttttgagg tgttagctgg
    66121 tctgaacttg aacgaactgc tccaaacagg tcatccaaac gttccaaggc caaactagca
    66181 tcaaagagca attcaatttc gccatttaat tcgatagtac gatactggag cagtcccctt
    66241 tccgtcagat tagaaatgga ctggttgaca tccgaaattt ccttgccaat cctttcagca
    66301 atctggcttg gcgacatttc ttctaagcct gtcgtatttt gcaaatagaa aaattgccaa
    66361 accagaaaat cgtcgctaga aggaaagagt tccttaaaat gcaagagcag ggcactcggt
    66421 aaaaccaagg tacctgattt aaaagcgtct aaatatgtca taattcctct tataaatacc
    66481 catatgcaga tgcatcatct tctatctttc tccaatcaaa aggcgtctcc tgatagaccg
    66541 catggtttac ccaattactg aaaaagaggg ctgcagatga agaccaacaa agacaaggta
    66601 cctgattaac atcatcatct ttaaagtaat tttctggaat atgtgggtcg aaacctgcat
    66661 cacgatctcg aaaatactct tttgccaagg tatcacggtc atactccaaa tgaccaaaac
    66721 tataaatttc tcgtaaatca cgactggcca aaatagaaac cccaacctga ggtccttctg
    66781 ataaaatctc gagattggtc ttgtttaaga cctcttcctt agaaatctcc gtgtgccgtg
    66841 aatgagggga tacatagcta tcatcaaagc ctctaaatag aaggtgaccc tcttttaggg
    66901 tgtcctgagg ataaatacct gatagcttac tgtccatctg gtatttttct acaccatagc
    66961 gcagataaag cccagcctga gccccccaac agatatgaag ggtcgaatag acatgagtct
    67021 tagaccactc aagcatctgt ctaaattcct cccaatagtc cacttcctca aatggtaaat
    67081 gctcaactgg agcacccgtg atgatcatcc catcaaaata ctcatccttg acttcaggaa
    67141 aagttttata gaaggtctcc atgtgctctg aacgagttgt tttagaacgg tggctctcca
    67201 tatagagaaa atcaatatcc agttgtaggg gtgtattagc caagtggcgc aacaactggg
    67261 tctctgtgac cattttctgt ggcatgagat ttaaaattaa aatcttcaaa ggacggatat
    67321 cttggtgggc agcacgttga tcatccatga caaagatatt ctctgtccgt aaaatctcaa
    67381 cagctggcaa ttttttatca attcgaatcg gcataaccct ctcctaactg tactctttca
    67441 ggaataattg catatgtttg aagacaaatc tcaaacactt agtcctttta ttatactgca
    67501 agaagatata gttttcaatt atagtttttc tctaactagt tatagtctat ttttatatcc
    67561 tagtgtagag aaaacagccc tagggactgt tttcattaat aatgcataag aactttgtag
    67621 tcgtagtcac caattttttc acggccgttc aattcatcca attcaacaag gaaggcacaa
    67681 cctgccataa caccaccaag tttttcaatc atctcgatag ttgccttaac agttccacct
    67741 gtcgccaaaa ggtcatctac aataagaaca cgttgacctg gcttaatggc atccgcgtgc
    67801 atagtcaagg tatcgacacc gtactctttt tcatagtcag cagaaataac ttcgcgtggc
    67861 aatttacctg gcttacgaac aggcgcaaaa ccaattccca actcaaaggc aactggacaa
    67921 cccacgataa atccacgagc ttcaggtccc acgatcatgt cgactttctt gtcagtagca
    67981 tactgaacga tttcacgaac agcgtagcta taagcatttc catcagccat caaaggacta
    68041 atatcacgga aggtaatgcc ttcctttgga taattttcaa ttgttgcaat gtaatctttt
    68101 aaattcatct ttttctttct ttcaaagttt tttactctct attatagcat attttttaag
    68161 aaagaaaaaa ggaaaagtta acttcaataa ttatctaacg ttttgacgat ttataactag
    68221 ccatcgcaat aaagcccaat ttctgtttat tcttagcaaa cattttatac atagttaaaa
    68281 actgctttct attctccttt ttacaagcat ttacacaaat tttcaaagtt cctagcaaac
    68341 cttcgtcata aatcataccc gataatttca ttaatgtcat ttcaccagtc aatgctttca
    68401 catcacaata acctgattct atcatcacct gttcccaacc atcttgagtt aaaggaccta
    68461 catttacatg aattgcttgt gataattcct gtctgataga ctctttagct tccttaagaa
    68521 gcacatcatg tgtcaagaga agacctccag gttttaatac ccttagatat tccattacac
    68581 attttttctt agcttgatcg gcttgcatag tcagcatagc ttcatttata acaatatcaa
    68641 aactagcatc ttgataagga agtttcattg catttgctct ttcaaaactg attaaatgag
    68701 caacacctgc cgttccagca gattttttag ccacttctaa agcttgagca tccatatcaa
    68761 cagcagttat cttgcaacca aaacgctgtg ccaactcaat tgctgtagtt cccctattac
    68821 acgcaacctc tagtattctc ttttcttttg aaaatcctcc ttctgcaatt aaccaatctg
    68881 tggcacgctt tccacctgga cgtaagcgtt ttttccccaa ttttgctaaa aacttatgac
    68941 ctgcttctga catttgaaca cctctgtttt ttcttcatta taacacaaaa tgatattatc
    69001 tgacaattct aatctattat ttgataaact agaatcaaaa gcatacaagt caccataact
    69061 atagcataaa tttattatta cctctctagt tcgaattcaa ttgctcacgt agtttacgca
    69121 tatagcttca cttgtagatg gtacccacaa ataatactca atggaaatca aagagcaaac
    69181 taggaaacta gccgtaagct gctcaaagta catctttgag gttgcagata aaactgacga
    69241 aatcagtaat catacgtacg gcaaggcgac gttgacgtgg tttgaagaga ttttcgaaga
    69301 gtattaaaga gcatcacaat atagatgcat aatatgactt ctctagcaaa aaggagagaa
    69361 gcattttgtt tctctcccta gattaactga tatctctcca ctacaattga taaagagata
    69421 tcttttcttg tagtgctttg aattttattt attttagtag attgtttttt cagtttctac
    69481 atcgaagaag tgtgctttgt tcaagtcaaa tccaagctca actgttgcac ctgtttgcaa
    69541 gtagtcacga gcatcaactt ttgcaacaaa ctcgtcttta ccaacttggc agtaaaggtg
    69601 agattctgaa ccaagcagtt ctgatacaga gatagtcgct tttacaacac agtctgggaa
    69661 tgtttcaagg aaagcaggtt ctgcattcac gtcttctgga cggataccaa agatcaattc
    69721 ttttccttcg tagccttttt cacgaagaac tttcaatgct ccttctggca ctttcaaacg
    69781 gaaaccgtca gaaacaattt cgctaccaac caatttcacg gtgatgaagt tcatagctgg
    69841 gcttccgatg aatcctgcaa cgaatttgtt aactggattt ttgtaaactt cttgaggagt
    69901 accgatttgt tctacacgtc cgatagtacc tgtaccagca gggttcttag tagctgacat
    69961 aataacgata cggtctgcaa gtgtcatcgc ttctgtttgg tcgtgagtta catagatagt
    70021 tgtagctccg atacgacggt gaattttagc gatttcagca cgcattgata cacgaagttt
    70081 ggcatccaag tttgacaaag gttcgtccat caagaatact ttcgcatcac ggacaatcgc
    70141 acgccccatg gcaacacgtt gacgttgacc acctgaaagg tcagctggtt tacgttccaa
    70201 gaattctttc aatccaagta tttcagctgc ttcttgaaca cgtttgttaa tgtcttcttt
    70261 gctgtattta cgcaatttca aaccgaaagc catgttgtca taaacagtca tgtgtgggta
    70321 aagagcgtag ttttggaata ccatggcgat atcacggtct tttggagcta cgtcgttgac
    70381 aactacgcca tcgatagatg cagtaccttc tgtaatgtct tcaagaccag caatcatacg
    70441 gagtgtagtt gatttaccac atcctgaagg tcctacgaaa acgataaatt ctttatcttt
    70501 gatgttcaag ttgaaatctt caactgaata gtgttcgctg tttggatatt ttttgtaaat
    70561 atttttaaga ttcaattcta ccatgaggtg aactcctttt gtcttttgat aattatatta
    70621 tatatgaaaa cgctttatat ttctatggca agatgaccaa aaaaataaac aagttctgtg
    70681 caacttgcac aaaactttag agaatatcgg agaatttgaa gaagataatc tttattattc
    70741 ttctatgaca aaagcagaaa caaggatcag ttttcccata ttttcgctga ttctccacta
    70801 catttgactg gattctaatt ttttagagaa atacaaaaga gctagcttta gctagctctt
    70861 ttcctatgcg gagagaggga cttgaaccct cacgacctaa agcggtcaca ggatccttag
    70921 tcctgcgcgt ctgccaattc cgccatcccc gcgtcgatta ctttactagt atatcaactt
    70981 ttgggatgct tgtcaacact ttttttcaaa ttttttcatt ttcaccaacc aggttactca
    71041 gaaagttcat ttagattttc atctactaac ttagctccga gtgtattttt gaaatgacct
    71101 agggcaaatt gatgattttc aggccagatg gaagcaacag ctggtttaac aatctcgatg
    71161 tcatatccta gattataggc atctatagct gtatgtagga cacagatatc cgtcaagaca
    71221 cctgttaaga taacggtaga cactctacgc tctctcaaac gaatatctag gtcagtccct
    71281 gaaaaagctg agtaatggcg tttatccatc caaaagacac gactgtctga accatgctct
    71341 tgataaaaga tccccaaatc tccatataaa ttccgtccac tcgtcccaat cagattatga
    71401 ggaggaaata acttactttc cggatggaaa caatcgtttt cttcatgagc atcaatagta
    71461 aagaagatat aatctcctcg ttcaaaagct aatcgagtta ccttgctgat ggcatccgaa
    71521 atcgcctgag ctggagcacc tgctgttagt ttcccactat cagcaacaaa atcttctgta
    71581 taatcaatcg aaattaaagc ctttgtcatt agtaatctct tttcttcact tcttcaaaaa
    71641 tatctgaaat caagacctta agataggttc ccttcattcc aagtgagcga ctttcaataa
    71701 tccccgcaga ctcaagttta cgaagagcat tgacaatcac agagcgagtg attccgatac
    71761 gatctgcaat cactgacgca gtcaacttcc cttcatttcc atttaattcc cctaaaattg
    71821 ctgaaacagc acggagttcg gagtaagaaa gggtattgac cgccatggtg acagcagtac
    71881 gacgacgaat atttttctca tcttcttcac gttggaagtt aagaagctga atcccaacaa
    71941 cggtactggc aatctcaaca agaaccaagt cctcatcttc gaatttttta tcattacgcc
    72001 aaataatcaa agaaccaagg cgaatccccg atacatgaat cggtgcaata gtcgtcaagc
    72061 catctggaaa atcatctcta ctctcaacag ggaaaatact catatcatgc tcaacaggta
    72121 agtttgcttc tgtttcgtaa atcatattag ccccttgaac gtagtcatct gggaaaatct
    72181 tagtttggaa gaattgctct acgcgatctg tatttgtttt ataacgcata aaatagccaa
    72241 gcagacgtcc cttactattg ataatgcagg cattgcaatg aataatatcc gctaactgac
    72301 gcgtaatagc gttgtaagga agctcatcct gcaactgctc ctctgagcgt ttcaaaattg
    72361 atgtaatttt tctagttttt tctactaaat gtgccatttt tcacctcgaa tttaatcgct
    72421 atcattataa cataaaaacg tctctttttc aataattatc tgaaaattcc ttattgactt
    72481 gcattgactt acaatttaat taaaaaccag aatattttta attaaattgt tccttttcta
    72541 ttgacaagtt gcctattttt gtgtatcata acattataaa agataatata ataattttat
    72601 ttgtcttttc acattcggtc tccttatata aaaaagcgat tcattttgaa ccgctttttc
    72661 ttatttatcg cccttgttac gaataacaaa gcctgtttgc ttttcgctta aagtattgcg
    72721 tggtttttta ttatccttac ggtaacgttt ttccttatca aaacgatcgt tgccacgact
    72781 tccttttttg aactcatcac ggcgatcacg ctctcgacgg tcgtccccac gacggcctcc
    72841 acgacctccc ttagctttac caccgaaacc attacctgat ggtttaaacg gtagtggttt
    72901 ttcacgtgca atctccactt ctggaaggct atctgggtct tggactgtca gactcaagat
    72961 atacattgcc aattcttctg gagtaaactc agcagccaat ttgcgagcat ccttaccaaa
    73021 tttctcaaag ttggcacgaa tggtttcatc tgcaaaatca cgttcgattt tcttgagagc
    73081 tacctgtttt tttgattgga aggattcttc tacacttgca ggtttgagac ctttcatgcg
    73141 tttcttagtc aagttttcaa tgatttgaag gtaacccatt tcgtttggag caacaaaagt
    73201 aatagattga cctgacttac cagcacgacc tgtacgaccg atacggtgaa cataactctc
    73261 aggatcttgt ggaatatcgt agttgtagac atgggtcaca cctgaaatat ccaaaccacg
    73321 cgctgcaacg tctgtcgcaa ccaaaacatc aagattgcca tttttaaagt cacgaaggac
    73381 acgaagacgt ttgttttggt ctaggtcgcc atgaattcct tctgcacgga agccacgaat
    73441 tttcaaacca cgagtcaatt catccacacg gcgtttggta cgaccaaata caatagcgag
    73501 ttctggttgt gccacatcca tgagacgagt catggtgtca aatttttctt gttccttaac
    73561 acggatatag tactggtcaa ccaattctgt tgtcaattcc ttagccgcaa tcttgacatg
    73621 ttcaggggct ttcataaact gaacaccgat acgtttgatg gcatctggca tagttgctga
    73681 gaaaagcaaa gtttgacggt tctcaggtac acgggaaata atggcttcga tgtcttcaag
    73741 gaagcccatg ttaagcattt catccgcttc gtcaaggata agggtttcaa tgtcttgtaa
    73801 tttcaaggcc ttgcgtttaa tcaagtccaa gaggcgacct ggagttccca ccacgatatg
    73861 ggcaccagat ttaagagcct taatttgttt ttcaatgctt gatccgccat atactgaacg
    73921 gactttgact cccttactac gaccaaagcg gaagagttct tcttgacttt ggacagctag
    73981 ttcacgagtt ggagcgatga ccaaggcttg gatagtcgct tcttctgtac ggattttttc
    74041 aagggtaggc aagccaaagg ctgcagtttt tcctgtacca gtctgagctt gaccgataac
    74101 atccttgcct tcaagggcca aaggaatagt ttgttcttgg ataggactag cttctacaaa
    74161 accagctttt tcaatttctg ctagcaaatc agcagacaag tttaattcat taaatttcac
    74221 gttattcttc tttctaaagg tggtgcgaag ccaccctata gggcttagtt tatacttttc
    74281 tttttatgac gtattttcat ataactagat ataaaatcgt gttgcttctt ttccacaaaa
    74341 gaaaagtact gttttctttg caacctatct agtataacac aagaccagag caaaagatag
    74401 ccccatttct acagaaaatc atgtaagcgg tttttgactt tcttttttga ttgaacgacc
    74461 tagataataa gacaaagcca aggcgatact gtataaaatg agaaaaacga acaaggtttg
    74521 tgtgtacgaa tgagccattt tataagtctc tgctaataaa ataggtcccg ctaaaccagc
    74581 cattgcccaa gctgttaaaa tataaccatg cagagcggcc aattccttgg ttccaaaaat
    74641 atcactgaga taagctggaa tcaaagaaaa accagctcca tagcaagtca tcaaaataga
    74701 catagcaact acaaataaaa cggaatctgt aaagagccaa agtgagagag aaaagaaaag
    74761 attgacaagc agtaatatac taaaggttag agggcgaccg atatagtcag acaaactcgc
    74821 ccagagcaag cgaccaaatc cattgaaaat ccccaaaaca cccaccatta ctgctgcatg
    74881 acttgtagac aagccagcca tctcctgtgc cattggcgat gccgctgaaa ttaagcctaa
    74941 accacaagct atgttgataa agaaaataat ccaaagcata taaaaccgat tgctttttag
    75001 agcctgattt gcagccattc cttgcgtcaa agaggctgtt ttttctttcc ctgaagaaga
    75061 taaaattgca agctcttgct catttggacg cttaatgaat tgtgaagcta ggagcatgat
    75121 aataaagtaa cttgctccta aaatataaaa agtttctaca atccctaccc ctgcgatgag
    75181 gtgttgcgct atgggactag tcaataaaga agcaaaacca aaccccataa tcgctaaacc
    75241 tgttgcgaga ccacgtttat caggaaacca ttttataatc gtcgacacag gggtaatata
    75301 gcctgctccc aaaccaagcc cacctaaaat gccataagcg agatacaaca accacagctc
    75361 ctgacggtct attgcaaatc ctgctaagat atttccacct gcgtatagaa aagcagatag
    75421 acttcccatg actttcggac caaatttttc taccaaacgc cccataaatg cagccgataa
    75481 gcccaaacaa aagattgcta gactaaaggc gaaggcaaca gaagcctgat cccatcccgt
    75541 tttttcaata atagggttac gataaacact ccaggcataa gtcgaaccca gcattaagtg
    75601 taaaatgacc ccagcaaagg caataatata acgattcgat ttcataaaac ctcctatttt
    75661 cgaacattta ttctatctta tataaaaata gataggattt tagtttatca agcttgttaa
    75721 aaaattacta gttttttgtc tggaaagcgt ttttatcttt ttctgtcttt ttcgtagcag
    75781 aattgtgcaa aagttttttt cttgtgctag aatgaaatgc gaataggagg aattctaaat
    75841 gttaacttat gatttaatcg ttatcggatt tggtaaagct ggtaaaacac tagctggtaa
    75901 attggcttca gctggcaaaa aagttgccct cgttgaacgt agcaaagcta tgtacggtgg
    75961 aacttgtatc aacatcggtt gtatcccaac taaaactttg ctggttgctg ctgagaaaga
    76021 cttgtctttt gaagaagtca ttgctaccaa aaacacgatc actggtcgcc tcaacggtaa
    76081 aaactatacg actgttgctg gtactggcgt cgatatcttt gatgctgaag ctcacttcct
    76141 ttcaaataaa gtcatcgaaa tccaagctgg tgatgaaaaa caagaattga ctgctgaaac
    76201 tatcgtcatc aacactggtg ctgtttcaaa cgtcttgcca atccctggac ttgctacaag
    76261 caaaaacgtc tttgactcaa caggtatcca aagcttggat aaattgcctg aaaaacttgg
    76321 agtccttggt ggcggaaata tcggtcttga atttgctggc ctttacaata aactaggaag
    76381 caaggttaca gtcctagatg ccttggatac attcctacct cgtgcagaac cttccatcgc
    76441 agctcttgct aaacaataca tggaagaaga cggtattgaa ttgcttcaaa atatccatac
    76501 tactgaaatt aaaaacgacg gtgaccaagt gcttgtcgta actgaagacg aaacttaccg
    76561 tttcgacgcc cttctctacg caactggacg caaaccaaat gtagaaccac ttcaacttga
    76621 aaatacagat attgaactaa ctgaacgtgg cgctattaaa gtagataaac actgtcaaac
    76681 aaacgttcct ggtgtctttg cagttggaga tgtcaacggt ggtcttcaat ttacttacat
    76741 ttcacttgat gacttccgtg ttgtttacag ctaccttgct ggagatggca gctacacact
    76801 tgaggaccgt ctcaatgtgc caaatactat gttcatcaca cctgcacttt cacaagttgg
    76861 tttgactgaa agccaagcag ctgatttgaa acttccatac gcagtgaaag aaatccctgt
    76921 tgcagccatg cctcgtggtc acgtaaatgg agaccttcgc ggagctttca aagctgttgt
    76981 taatactgaa acaaaagaaa ttcttggtgc aagcatcttc tcagaaggtt ctcaagaaat
    77041 catcaacatc attactgttg ctatggacaa caagattcct tacacttact tcacaaaaca
    77101 aatcttcact cacccaacct tggctgagaa cttgaatgac ttgtttgcga tttaagttga
    77161 aatctcatct taactgacag ccctctttgg gctgttttta cttctacgaa acaccaaatc
    77221 tgtcttttcc ctcttttgtg atataataga aacatgaact taaaaactac tttgggcctt
    77281 cttgctgggc gttcttccca cttcgtttta agccgtcttg gacgtggaag tacgctccca
    77341 ggtaaagtcg cccttcaatt tgataaagat attttacaaa acctagctaa gaactacgag
    77401 attgtcgttg tcactggaac aaatggaaaa accctgacaa ctgccctcac tgtcggcatt
    77461 ttaaaagagg tttatggtca agttctaacc aacccaagcg gtgccaacat gattacaggg
    77521 attgcaacaa ccttcctaac agccaaatct tctaaaactg gaaaaaatat tgccgtcctc
    77581 gaaattgacg aagccagtct atctcgtatc tgtgactata tccagcctag tctttttgtc
    77641 attactaata tcttccgtga ccagatggac cgtttcggtg aaatctatac tacctataac
    77701 atgatattgg atgccattcg gaaagttcca actgctactg ttctccttaa cggagacagt
    77761 ccacttttct acaagccaac tattccaaac cctatagagt attttggttt tgacttggaa
    77821 aaaggaccag cccaactggc tcactacaat accgaaggga ttctctgtcc tgactgccaa
    77881 ggcatcctca aatatgagca taatacctat gcaaacttgg gtgcctatat ctgtgaaggt
    77941 tgtggatgta aacgtcctga tctcgactat cgtttgacaa aattggttga gttgaccaac
    78001 aatcgctctc gctttgtcat agacggccaa gaatacggta tccaaatcgg cgggctctat
    78061 aatatctata acgccctagc tgctgtggcc atcgcccgtt tcctaggtgc cgattcgcaa
    78121 ctcatcaaac agggatttga caagagccgt gctgtctttg gacgccaaga aacctttcat
    78181 atcggtgaca aggaatgtac ccttgtcttg attaaaaatc cagtcggtgc aacccaagct
    78241 atcgaaatga tcaaactagc accttatcca tttagcctat ctgtcctcct taatgccaac
    78301 tatgcagatg gaattgacac tagctggatc tgggatgcag actttgagca aatcactgac
    78361 atggacattc ctgaaatcaa cgctggcggt gttcgtcatt ctgaaatcgc tcgtcgcctc
    78421 cgagtgactg gctatccagc tgagaaaatc actgaaacga gtaatctgga gcaagttctc
    78481 aagaccattg agaatcaaga ctgcaagcat gcctatattc tggcaactta tactgccatg
    78541 ctggaatttc gtgaactgct ggctagtcgt cagattgtta gaaaggagat gaactaatgg
    78601 tttatacttc actttcctca aaagatggca attaccccta tcagctcaac attgcccacc
    78661 tctacggaaa tctcatgaat acctacgggg acaatggaaa catcctcatg ctcaagtatg
    78721 tggctgaaaa actgggagcc catgtgaccg ttgacatcgt ttctctccat gatgactttg
    78781 atgaaaatca ctacgacatc gcctttttcg gtggtggtca agactttgaa caaagtatca
    78841 ttgcagacga cctacctgct aaaaaagaga gcattgacaa ctacatccaa aacgacggtg
    78901 tagttctggc tatctgcggt ggtttccaac tattgggtca atattatgtt gaagcttcag
    78961 gaaaacgtat cgaagggcta ggggtcatgg gacactacac gctcaaccag accaataacc
    79021 gttttatcgg tgacatcaag attcacaatg aagatttcga tgaaacctac tatggatttg
    79081 aaaatcacca aggccgtacc ttcctctctg atgaccaaaa accgctggga caggttgtct
    79141 atggaaatgg aaacaacgaa gaaaaggtcg gtgaaggggt tcattataag aatgtctttg
    79201 gttcctactt ccacgggcct atcctctctc gtaatgccaa tctggcttat cgcctagtta
    79261 ctactgccct caagaagaaa tatggtcagg atatccaact ccctgcctat gaggatatcc
    79321 tcagccaaga aatcgctgaa gagtacagtg acgtcaaaag caaggctgac ttttcttaaa
    79381 caaaggaaaa tgatatcaaa gaactccgtt atcttgtcgg agttttttgt cttttctttt
    79441 acccttctcc cttgcatttt ctctcatttt ttgccaaaat agaggggtag aaagaaggta
    79501 gcatatgtct aaattacaac aaatcctaac atatcttgaa tcagaaaaac tagacgtcgc
    79561 tgtcgtatct gaccccgtca caatcaatta cctcactggt ttttacagtg atccccatga
    79621 acgccaaatg ttcctctttg tcctagcgga tcaggaacct ctcctctttg tcccagctct
    79681 tgaagtagaa cgtgcaagta gcaccgtttc cttcccagta gtgggctatg tcgattctga
    79741 aaatccatgg caaaaaatca aacatgctct tccacaactt gacttcaaac gtgtcgctgt
    79801 tgagtttgac aatctcatct tgaccaaata ccatggtttg aaaacaattt ttgaaactgc
    79861 tgagtttgaa aacctcactc ctcgtatcca acgcatgcgc ctcatcaaat cagctgatga
    79921 agtacaaaaa atgatggttg caggtcttta tgctgacaag gctgttcatg ttggttttga
    79981 caatatttct cttgataaga ctgagacaga tatcatcgca caaattgact ttgccatgaa
    80041 acgtgaaggt tacgaaatga gctttgatac catggtcttg actggtgata atgctgcgaa
    80101 tccacacggc attccagcag ctaataaggt tgaaaatgat gctcttctcc tctttgacct
    80161 gggggttctg gtcaatggct atgcgtcaga tatgactcgt acagtcgctg tcggcaaacc
    80221 agaccaattc aagaaagata tttacaactt gactcttgaa gcccaacaag ctgctcttga
    80281 ctttatcaag ccaggtgtga ctgctcatga agtggaccgc gctgcccgtg agatcatcga
    80341 aaaagctggt tatggtgagt acttcaacca ccgtctcggg catggtatcg gtatggatgt
    80401 ccatgaattc ccatctatca tggaaggaaa cgacatggtc atcgaagaag gcatgtgctt
    80461 ctctgttgaa ccaggtatct atatccctgg taaagtcggt gttcgtattg aagactgcgg
    80521 tgttgttacc aaggatggct tcgacctctt tacaagcacc agcaaagatt tgctttattt
    80581 tgattaaact atatagcccc tatgctttcc tttcaaaata tctaggggct attttattgt
    80641 catttttctg ctattatgct aaagaaattg gctgcaataa tctaacccta agtgtctgga
    80701 atgataacga gggtgctctc cgcttttatc aaagacaagg gatgaaaccc caagaaacaa
    80761 caatggaaat gataattgat taagaagtca tctatcaaaa gatgttagaa aaagttcaat
    80821 ttcactagaa aatgaggaaa atcttcccac aataaaacgc ataatagtgg gtgtagaatg
    80881 aactgccccc caaaaagtta gacaattaat ttatccgaag gatttagttc tgtattgcac
    80941 agggataagt ccttttagtt ttaccttaat tcgtttgttg ttgtagtaat caatatagtc
    81001 tataatggct tgttccaatt ggttaagtga tttaaatgtt ttctcatagc cataaaacat
    81061 ttcggatttc agaatcccaa agaaggactc catcataccg ttgtcttggc tgtttccctt
    81121 gcgtgacata gatgcttgaa ttcccttact ctctaggaac cgatgataag aatcgtgtcg
    81181 gtattgccag ccttggtcac tatggagaat cgtattctcg tagtgcttct ctttgaatgc
    81241 ctgttccaac attgtttgta cttgttctaa attaggcgaa caagaaagat tataggcgat
    81301 aatttcgctg ttaaagccat ctaaaactgg tgataagtaa agcttttgag tacttgctgg
    81361 aatggcaaat tcagtcacat ctgtgtagca ctgttccatt gttttagagc cttcaaattg
    81421 gccttgaatg agattctctg ccttcttacc aacgtctcct ttattttcgt ttctgtcgca
    81481 ttttagcttg taaattgagt actttcatca agccttgaac tcttttatga ttt
//
LOCUS       DAVKJU010000002        51591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVKJU010000002 DAVKJU010000000
VERSION     DAVKJU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.002"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(18..1277)
                     /locus_tag="VW191_000078"
     CDS             complement(18..1277)
                     /locus_tag="VW191_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEU9622931.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPEESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     gene            complement(1624..3372)
                     /locus_tag="VW191_000079"
     CDS             complement(1624..3372)
                     /locus_tag="VW191_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9622932.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLVIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(3374..5098)
                     /locus_tag="VW191_000080"
     CDS             complement(3374..5098)
                     /locus_tag="VW191_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9622933.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(5156..6094)
                     /gene="msrB"
                     /locus_tag="VW191_000081"
     CDS             complement(5156..6094)
                     /gene="msrB"
                     /locus_tag="VW191_000081"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9622934.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(6242..6313)
                     /locus_tag="VW191_000082"
                     /pseudo
     CDS             complement(6242..6313)
                     /locus_tag="VW191_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6389..7258)
                     /locus_tag="VW191_000083"
     CDS             complement(6389..7258)
                     /locus_tag="VW191_000083"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEU9622935.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            complement(7260..8546)
                     /locus_tag="VW191_000084"
     CDS             complement(7260..8546)
                     /locus_tag="VW191_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEU9622936.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(8697..9434)
                     /gene="mecA"
                     /locus_tag="VW191_000085"
     CDS             complement(8697..9434)
                     /gene="mecA"
                     /locus_tag="VW191_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEU9622937.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(9634..10668)
                     /locus_tag="VW191_000086"
     CDS             complement(9634..10668)
                     /locus_tag="VW191_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9622938.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(10697..12130)
                     /locus_tag="VW191_000087"
     CDS             complement(10697..12130)
                     /locus_tag="VW191_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEU9622939.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            complement(12146..13132)
                     /locus_tag="VW191_000088"
     CDS             complement(12146..13132)
                     /locus_tag="VW191_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9622940.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(13134..14237)
                     /locus_tag="VW191_000089"
     CDS             complement(13134..14237)
                     /locus_tag="VW191_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9622941.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(14227..15072)
                     /locus_tag="VW191_000090"
     CDS             complement(14227..15072)
                     /locus_tag="VW191_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9622942.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGTLR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSYKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKDPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(15351..16625)
                     /gene="psr"
                     /locus_tag="VW191_000091"
     CDS             complement(15351..16625)
                     /gene="psr"
                     /locus_tag="VW191_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEU9622943.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(16622..17470)
                     /gene="pheA"
                     /locus_tag="VW191_000092"
     CDS             complement(16622..17470)
                     /gene="pheA"
                     /locus_tag="VW191_000092"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEU9622944.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEGVTIPLQSQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(17467..17943)
                     /locus_tag="VW191_000093"
     CDS             complement(17467..17943)
                     /locus_tag="VW191_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU9622945.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(17936..19219)
                     /gene="aroA"
                     /locus_tag="VW191_000094"
     CDS             complement(17936..19219)
                     /gene="aroA"
                     /locus_tag="VW191_000094"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEU9622946.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSASFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(19311..19649)
                     /locus_tag="VW191_000095"
     CDS             complement(19311..19649)
                     /locus_tag="VW191_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEU9622947.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(19660..20763)
                     /locus_tag="VW191_000096"
     CDS             complement(19660..20763)
                     /locus_tag="VW191_000096"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEU9622948.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLESIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITKNTDYTVVIK"
     gene            complement(20773..21939)
                     /gene="aroC"
                     /locus_tag="VW191_000097"
     CDS             complement(20773..21939)
                     /gene="aroC"
                     /locus_tag="VW191_000097"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU9622949.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLLLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(21949..23016)
                     /locus_tag="VW191_000098"
     CDS             complement(21949..23016)
                     /locus_tag="VW191_000098"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU9622950.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(23035..23889)
                     /locus_tag="VW191_000099"
     CDS             complement(23035..23889)
                     /locus_tag="VW191_000099"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU9622951.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEAHLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            complement(23879..24556)
                     /locus_tag="VW191_000100"
     CDS             complement(23879..24556)
                     /locus_tag="VW191_000100"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEU9622952.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(24553..25716)
                     /locus_tag="VW191_000101"
     CDS             complement(24553..25716)
                     /locus_tag="VW191_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU9622953.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFISKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(26401..28035)
                     /locus_tag="VW191_000102"
     CDS             complement(26401..28035)
                     /locus_tag="VW191_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9622954.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMIICFSNIAIHFPFEEIPIYFSLMIAILLVIAFSTSLTAFYNVFYESK
                     DLISYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVDPFSTEAILGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKEFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(28016..28765)
                     /locus_tag="VW191_000103"
     CDS             complement(28016..28765)
                     /locus_tag="VW191_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9622955.1"
                     /translation="MIEFQNVSKLYGDKKALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVADASQGH"
     gene            28944..30398
                     /locus_tag="VW191_000104"
     CDS             28944..30398
                     /locus_tag="VW191_000104"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEU9622956.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPSAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(30488..33106)
                     /gene="alaS"
                     /locus_tag="VW191_000105"
     CDS             complement(30488..33106)
                     /gene="alaS"
                     /locus_tag="VW191_000105"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU9622957.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNHNASQLSSKLVAIVADNAEVEAVSEGTTSLIFAETPFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQPKIQELLDAVAGKL"
     gene            complement(33128..33613)
                     /locus_tag="VW191_000106"
     CDS             complement(33128..33613)
                     /locus_tag="VW191_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEU9622958.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(33888..34127)
                     /locus_tag="VW191_000107"
     CDS             complement(33888..34127)
                     /locus_tag="VW191_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9622959.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(34388..35458)
                     /locus_tag="VW191_000108"
     CDS             complement(34388..35458)
                     /locus_tag="VW191_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9622960.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGSEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(35455..36228)
                     /locus_tag="VW191_000109"
     CDS             complement(35455..36228)
                     /locus_tag="VW191_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9622961.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(36225..37031)
                     /locus_tag="VW191_000110"
     CDS             complement(36225..37031)
                     /locus_tag="VW191_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9622962.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(37012..38169)
                     /locus_tag="VW191_000111"
     CDS             complement(37012..38169)
                     /locus_tag="VW191_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9622963.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(38330..39235)
                     /gene="murB"
                     /locus_tag="VW191_000112"
     CDS             complement(38330..39235)
                     /gene="murB"
                     /locus_tag="VW191_000112"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU9622964.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(39347..40099)
                     /locus_tag="VW191_000113"
                     /pseudo
     CDS             complement(39347..40099)
                     /locus_tag="VW191_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
     gene            complement(40109..40840)
                     /gene="budA"
                     /locus_tag="VW191_000114"
     CDS             complement(40109..40840)
                     /gene="budA"
                     /locus_tag="VW191_000114"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEU9622965.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     VGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            40982..42253
                     /locus_tag="VW191_000115"
     CDS             40982..42253
                     /locus_tag="VW191_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEU9622966.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(42273..43088)
                     /locus_tag="VW191_000116"
     CDS             complement(42273..43088)
                     /locus_tag="VW191_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9622967.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(43245..43898)
                     /gene="phoU"
                     /locus_tag="VW191_000117"
     CDS             complement(43245..43898)
                     /gene="phoU"
                     /locus_tag="VW191_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9622968.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(43910..44668)
                     /gene="pstB"
                     /locus_tag="VW191_000118"
     CDS             complement(43910..44668)
                     /gene="pstB"
                     /locus_tag="VW191_000118"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9622969.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLNPQHKETEDYITGKFG"
     gene            complement(44681..45484)
                     /gene="pstB"
                     /locus_tag="VW191_000119"
     CDS             complement(44681..45484)
                     /gene="pstB"
                     /locus_tag="VW191_000119"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9622970.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQALDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(45495..46379)
                     /gene="pstA"
                     /locus_tag="VW191_000120"
     CDS             complement(45495..46379)
                     /gene="pstA"
                     /locus_tag="VW191_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9622971.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(46369..47286)
                     /gene="pstC"
                     /locus_tag="VW191_000121"
     CDS             complement(46369..47286)
                     /gene="pstC"
                     /locus_tag="VW191_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9622972.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(47286..48164)
                     /gene="pstS"
                     /locus_tag="VW191_000122"
     CDS             complement(47286..48164)
                     /gene="pstS"
                     /locus_tag="VW191_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU9622973.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(48738..50042)
                     /locus_tag="VW191_000123"
     CDS             complement(48738..50042)
                     /locus_tag="VW191_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEU9622974.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(50043..50816)
                     /locus_tag="VW191_000124"
     CDS             complement(50043..50816)
                     /locus_tag="VW191_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU9622975.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNGRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGHLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(50806..51084)
                     /locus_tag="VW191_000125"
     CDS             complement(50806..51084)
                     /locus_tag="VW191_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEU9622976.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(51084..51485)
                     /locus_tag="VW191_000126"
     CDS             complement(51084..51485)
                     /locus_tag="VW191_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEU9622977.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
BASE COUNT        15278 a        11722 c         9797 g        14794 t
ORIGIN      
        1 ttttcgtaat ttttctttta aagttctact gtcaggactt gaccttgctt aacaacctgt
       61 tctccggcga tataaacatc atctacatca ctagatttaa ctgcataaac caggtgagac
      121 agcatatttt cctgaggttg gagatgaatt ttcccttgtg gttgaatgac cagaaaatct
      181 gcttgcttgc cgacttccag acttcctatc tgattttcca ttccaaggac cttagcccct
      241 tcgattgtca gtaccttgag agctgtttcg attggaaact ggctggcatc cccacttttc
      301 atcttctgaa gaagagctgc agtccttcct tcctcaaaca tatctagatt gttattggaa
      361 gcaaccgagt cagtcgcaat tccgactgct actcccgctt tttggagctg gataattgga
      421 gcaattcctg atgccagttt gaggttactg ataggattgt gggcgatagc cacttgagaa
      481 gatgccaagc gttcaatttc tctctcgttt aattcgaccc cgtgagcaaa tacggacgga
      541 tgatctaaat aacccagttc ttcaagaaaa gcaagggggc gtttgccgta tcgtttgagg
      601 ataattcctg actcctcctt ggtctccgcc acatggacat ggagcggaat atttagctct
      661 tttgccattt ccaaactcgc ttccagcaag tctctactgc agctatacgg agaatgaggt
      721 gctaccataa ccttgaaatt tggattttta tattttaaga tttcatctat gatggatcga
      781 gttctgctta tagtctcagc agttgtttct gtctctgaag aaaagagagt cggagaaaaa
      841 taacaacgca tcttggaagt tttcaccacc tgataaattt gctggatatc cacaccattg
      901 ggattataca tatcgttaaa ggttgttgtt cctgactgga gcatctctgt tagggcttct
      961 ttaaccgcat tggtagtcat gtcgggagta aactcagatt cttctggcca gatatagtca
     1021 ttgagccatt catggagatt gctgtcatct cggatccctc tcagacctgt cattgcagaa
     1081 tgggtgtgac aattgaccaa accaggcata atccaagctc cctgatagtc tataatctgc
     1141 tcagcttgtt ctaaaaacgc tggcttatct tgaccgacat agacgatttg agaatccttg
     1201 actgctaaga ttccatcaag ataaacatgg aaattttgat cacaagtcac gatatttaca
     1261 tgctgaaaga ctttcattat aggctccttt tctaaaagac aagttgtact ctcttcaaac
     1321 cacttcagat tcactttgcc gtaggtgtgg ttactgactt cgtcagttct atctgcaacc
     1381 tcaaaacagt gttttgagct gacttcgtca gttctatctg caacctcaaa acagtgtttt
     1441 gagctgactt cgtcagtctt atctacaacc tcaaagctgt gctttgagca acctacggct
     1501 agcttcctag tttgctcttt gattttcatt gagtattaca gtgaacaatt tttctagcta
     1561 ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc aaaagagaat
     1621 ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac cctttctatc
     1681 catgagttct tgatggttac catattcaac aatatcacca tctactaaga caagaattaa
     1741 atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc ggcccttcat
     1801 gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg aagaagttgc
     1861 ctcgtctaag ataagaatct ttggaataac cagaaagact cgggctatgg tcaagagctg
     1921 agcttggccg acagagagag attctccagc attttccaac ttggtatcgt atccctgtgg
     1981 caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta cttgctctcg
     2041 actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg tgagccaggt
     2101 ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat cataaatgga
     2161 ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa ggagattgat
     2221 aagagttgat tttccagcac ctgtcggacc aacgatggct atcttactac cagctggaat
     2281 atcgatagac aagtccttaa tcaaaatttt ttcaggatgg tagccaaaag agacatgttt
     2341 aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg tttcagccac
     2401 ttcagggcta tctaagactc catagatacg ctctacgcaa gccagagcac tttgcaactc
     2461 agctagcact gaagaaatat cattaaaggg cttggtgtat tgctgaacat agttcaaaaa
     2521 agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag ctcctactcc
     2581 agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga ccgttgaaga
     2641 ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac gcaatctttg
     2701 gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa gcgattcttc
     2761 aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat gataggatct
     2821 cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga ctaagagagt
     2881 catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca aaacaccaat
     2941 gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatgt ccgtggttac
     3001 acgactaacc atctctccac taccttgcct atctacaaaa gcaatcggta aacgatggag
     3061 cttatggatg attcgctctc gcaaatctct agtataagag aagattagac gattatagag
     3121 gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca tctggagaaa
     3181 aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga cctgcccaat
     3241 cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa aggctaggaa
     3301 aaggaggaag ggatggtttg ctaaatctac ggctaaacgt ttgagcgtct ggtttacagt
     3361 ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca cgatagactt
     3421 ggctggattt catcaagtca tcgtgcttgc caacagctag caactcacct ttttccaaga
     3481 ggagaatctg gtccgccatc tgtaaagttg aggttcgttg agagatcaaa attaagctcg
     3541 tgtttggaaa attttctcta atagctttca agagcttgga ctctgtaatg gtatccagtg
     3601 ccgaggttgc atcatctagg atgataaacg gagcttggcg caagactgct cgggcgatag
     3661 acaacctttg tttttgtcca cctgagaaat ttcgcccccc tgcctcaact agggcatcca
     3721 aaagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag gcctgccaga
     3781 gtttctggtc agatacttct tgattgaaac ctagagtcaa gttggaacga atggttcctt
     3841 taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc tccaaattaa
     3901 gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga taaagtccaa
     3961 gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata ccaaggattt
     4021 gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca tcaggatagg
     4081 taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt tgttctaatt
     4141 ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg gctgagatat
     4201 aggactggtt gagggaattg atcagcatgg caagcttgac caattccacc aaaatctgta
     4261 agaggtaatt gataagagca atgagagcac cttgactgag cactcctcct tgaattgaaa
     4321 tatagccttg ccagataata acgagaagag ttccattgac aatcagatag gtcagaggtg
     4381 ttaataaact agaccagaaa cctgtctttt cttgtaatct agcataaact tggttaaggg
     4441 tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc cgcatccctt
     4501 gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga ctactgtaga
     4561 gaggattgac caatcgagat agccctacaa tgacaatggt caaaatggca accatgacta
     4621 agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca ccaaaaacga
     4681 taatgggcgc tcgcaaaaag agacgcagga attgattgat accagtctga atctggtagg
     4741 tatccgaagt caagcgagtg accaaactag aagttgtcag acggtctctg ctgtccttgg
     4801 acaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaag cctactgctg
     4861 cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact gcaaagataa
     4921 ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa gattggtcaa
     4981 caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac agcttgaaca
     5041 agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg tgtttcataa
     5101 atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct ctgttttatt
     5161 tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc ttggccacaa
     5221 agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc ggtccatctg
     5281 tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt cgctccattc
     5341 catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg ctaaaacttg
     5401 gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca cctgtcgtaa
     5461 tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat ggagcctctg
     5521 tagcagcttc ttgtgtgaca cgataagact cttcagatag actggccttc aacacctctt
     5581 gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg gtcacatcga
     5641 tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct tcagccagaa
     5701 tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg cgttcctgct
     5761 cctgtaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa atcccagttc
     5821 gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg ataactcgga
     5881 aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc acttggaccg
     5941 tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact tgaccattag
     6001 cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc tctaggcccc
     6061 aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta tcacgttttt
     6121 aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc gtatatatgt
     6181 tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg acttcttcag
     6241 ttctatccac aacctcaaaa cactgttttg agcagcctgc ggctagcttc ctagtttgct
     6301 ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac cctctggtca
     6361 agagtttccc catcctatct tctattcttt attttgcttc tacacggaca ccttgggtat
     6421 caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct gccttaattg
     6481 ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca gaaaggtagg
     6541 ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct cttaccaagt
     6601 cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg gtcaccatgt
     6661 ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca acagcttcct
     6721 tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat tcatagtttg
     6781 gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag acttgccctt
     6841 caacagaact tgcaataacg agattaccat aaatggctgg agccacattg tcaggatgcc
     6901 cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt tgacccagtt
     6961 ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa ccgcgcgcca
     7021 aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca atttgcaaag
     7081 cgattttgag caagagatta cgctcatcat gtggaatcca tttgccaatc tggtgttcaa
     7141 tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt acagctacac
     7201 cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata atcttcatct
     7261 tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt cttcaattca
     7321 gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac gacacgcgcc
     7381 ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg agcattgaag
     7441 atttcagcca acttcaagac ctgacctttt gagtctggag ccaagattga gaaatagtag
     7501 tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc gttgaagtct
     7561 ttgccaatgg taccatcatt caaacgacga acgatacgga caatatcagc tacaacactt
     7621 gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc aataccgata
     7681 gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg cgctttcggt
     7741 aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat agaaccaacc
     7801 aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt gatattgcgg
     7861 attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa ttggctcaaa
     7921 ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc gctttctgca
     7981 aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc ttccaccatc
     8041 ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt aattttatca
     8101 gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac tgctgcttcg
     8161 tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc atggacagct
     8221 aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg agtgataaag
     8281 gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc ctggtctgat
     8341 aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag caagcgattt
     8401 ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg tgctgattga
     8461 ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac ggtaccaaat
     8521 cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa tatagccatt
     8581 ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta taaaaagaaa
     8641 aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag aaaggattag
     8701 ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc atactcttgt
     8761 aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc caacagccaa
     8821 gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata atagcgctcg
     8881 ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc aaaagctacc
     8941 aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt cttactctca
     9001 gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc aagagattga
     9061 atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt tttgataaat
     9121 tcatctggag acatttgagc caattcttcc atatctggca aatccgataa gtcttcaaaa
     9181 tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc aggttttgga
     9241 gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc tagctcatcc
     9301 aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc agcaatctcc
     9361 attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt atcactaatt
     9421 tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat actagatttt
     9481 tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg gtctttctat
     9541 aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa atcggctctt
     9601 agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg ctacaccgag
     9661 gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct tataacgagt
     9721 cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg atacatcctg
     9781 caaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt tatctaggat
     9841 agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg acaaaacatc
     9901 tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact gagaaaaatc
     9961 acctactcta ttgattgttg gattaaagag ataaactaac acatttccca tcacaaccaa
    10021 aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca catttaagcc
    10081 aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca gtttttcaat
    10141 tttcacattt gcataagcat gtttaaattt ctcaatcaac ccatcaattt ttttctctaa
    10201 caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg catcgtcaat
    10261 catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt tcaaggttgt
    10321 ttccgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta catcattgaa
    10381 aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac tataaccacc
    10441 aatttgatga ttcaaaatcg ttttggccga atcttctgga ggagtgattt tatagataag
    10501 ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg ctttaattgt
    10561 aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg cttttcgttt
    10621 tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga tttttttctc
    10681 ctttgtttag tagatactag ttttcctttg taagcatttt tgctacaaat ataatcacaa
    10741 gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa aagctgcttc
    10801 ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg attgtccttg
    10861 agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa ctaagcagaa
    10921 agactccaat cataccatag tcggtataca actccatgat ataactactt ccgataccat
    10981 gccctttcaa gtattccttg ttcaagacaa gataggatag attgtgggca taactattac
    11041 tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct ccgaaaatgg
    11101 ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa ttacggaaat
    11161 ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca aaactagtcc
    11221 cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgagaa acttggacat
    11281 tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa cctacaaaaa
    11341 tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc cgcataaagt
    11401 aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca attgccaaat
    11461 gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat ttctttggct
    11521 tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg taggtaaaat
    11581 aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc aaacgataca
    11641 agagccgttc aaataaccga atgaaataga aaggataggt tagaagaaaa actcctagtg
    11701 atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata tttgctactt
    11761 ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc aagcccagaa
    11821 tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag gtatccaaag
    11881 caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa atggttaaat
    11941 agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt aagctactaa
    12001 ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac agatacacaa
    12061 ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat aatcgtttcc
    12121 catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca atatttttca
    12181 gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta attcttctcc
    12241 ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa aaacgatgaa
    12301 tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta tccagaataa
    12361 agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga tagtttttca
    12421 caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc tctgcatagg
    12481 gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta gtattaacca
    12541 catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg taaatcaacc
    12601 ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg caaatcgtcc
    12661 caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca cagacaaaat
    12721 agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc atgacaccac
    12781 agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga atcatttctg
    12841 gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc tgcttcatcc
    12901 catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac accctgtcat
    12961 cttgttcagc gattgaatca cacaagcgac cactttcatc tgttgcacca tcatcaacaa
    13021 gaataatttc cagattttga taggtctgct tctgaatgga agctatcgat ttttctaggt
    13081 actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc atgctactcc
    13141 tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg aatgaattgg
    13201 ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat cgcctgagct
    13261 gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa ttcctcagcc
    13321 cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac atacactcca
    13381 ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagatag atactgataa
    13441 ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc tttgactcgt
    13501 tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata gttttttccc
    13561 tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt ttcctcaatc
    13621 cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc ttgagatttt
    13681 tctagaatag tctgaaaatc atatccattg tagattgtct gtaatttaga agcataatct
    13741 ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat tgtattcgca
    13801 gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag ttcttcaata
    13861 cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa cagtggtgga
    13921 ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa acgacgagcc
    13981 agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct ggtttggcga
    14041 taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata tcccttgtca
    14101 aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt tgaaacaatg
    14161 gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa aaataagatt
    14221 tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca tatcagcgat
    14281 gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat aatccccaaa
    14341 agcagttctg agatagacat catagccgat tggaataggc atctctgttc cttcaaatgg
    14401 caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt agccaggacc
    14461 tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca tttctttctc
    14521 agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta aaatgcgact
    14581 tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa agagtgaata
    14641 aatcaaggcc caacgaacct gttttttccg ctcagctgga tctttcggat aataatccaa
    14701 aggcaaaaca tccaaggcca gaccatgtgg caaatccaaa tcctgctgat aaggcttgat
    14761 acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa aatccttgta
    14821 actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg ctaatttctc
    14881 ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa atcccttgtt
    14941 tcgaagggta ccaatagcgc ctccgccaca gagataacag agcaaatcat gttctttaca
    15001 aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt ttaaatcagt
    15061 catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca aggggtgaaa
    15121 atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct atcaaacttt
    15181 gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt caccttgccg
    15241 taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt gtttttagca
    15301 gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc ttatctcaag
    15361 cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc caagcctcca
    15421 taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt ttcaatacca
    15481 ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata gggtacgttg
    15541 gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag tttagtggga
    15601 tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt acggccgtaa
    15661 tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc attcatctgc
    15721 tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcata taaatcatct
    15781 ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc atcaatcgtc
    15841 accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc aaccaaggcg
    15901 tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat ttctgcccct
    15961 ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc tgtctgtttt
    16021 ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg catgaaactg
    16081 actagcttca ttttcttatc tgagcccccg acatttaata ccataataga gtcagttcgt
    16141 gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat attaactcca
    16201 tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc agcagttttc
    16261 tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa agccacacag
    16321 accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg tcttttcttt
    16381 tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt cgcatagctt
    16441 ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt tcccctagca
    16501 agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt gagatagtga
    16561 atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg attttcttta
    16621 ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca cccagaattt
    16681 tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag tccttatcgg
    16741 tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt ggacgacttt
    16801 caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcttta taaagtgcac
    16861 ctggaaggtt gtcaggtagg gtcaaggcta aactcatttt ttcagtttgt gattgcaagg
    16921 gaatagttac accttcagct cccaaaaccc aaaaacgtgt gaaattggct tccatttcct
    16981 gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta ggtgcaactg
    17041 ctgcaaaggg ctgatctgga tgttcggaaa taaaacgggc cgcataagct gtactagctg
    17101 ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct tgagccaagg
    17161 cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc atcaactgct
    17221 gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga aaaagatagt
    17281 ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca gaatagttca
    17341 ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc tgcaattcct
    17401 catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga cctagataag
    17461 ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca catccaaaac
    17521 ccgactagcc acttcctcat accaagcctg tctttcttga aaaatagcta ctagttcttc
    17581 cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga tacgtaggta
    17641 gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga gtaagtcacg
    17701 atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg tttgtagtaa
    17761 atcagctagg acttctgatt ctacctgacg aaaggctgct tctccctttt cagcgaaaaa
    17821 attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa ggtaattagg
    17881 gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata acaccttagc
    17941 catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg atggcttctg
    18001 cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct gtcatcatgc
    18061 cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat ttccctttga
    18121 taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag gcgtctgcca
    18181 caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg ataactgtta
    18241 caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc aatcgtggaa
    18301 tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc aaggtagcag
    18361 atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca cgaatgacat
    18421 caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaaa cgagaatttg
    18481 ggacaatcaa acctgcgact aaccaaaagg atgcactgga aatatctcct ggtacgacca
    18541 ccttttgtcc agtcagtttt tgaggtcctt gaactgtgat tttcttgcca tccacactta
    18601 aatggccacc aaattgttgc aacatatctt cagtatgatt acgggtgcac tctttttcga
    18661 taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac ttgacttggg
    18721 cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct tttaagcgaa
    18781 ggggaggcaa gtctcgttca gtttgccctg agatgctgac gcccattttt ttcagtggaa
    18841 gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc tctacttcga
    18901 aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa tttcccatat
    18961 taagggcatt ttgcggcgct tttaagccag ccatgcctac accttgaatg gtaataaccc
    19021 catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc gaaagaacgt
    19081 cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt ccaaagataa
    19141 tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt aaatggcgaa
    19201 tatttgtttt tagtttcata ctggacctca tacttgcaat acttttacct attttatcat
    19261 aaaaagccag aaattcctta aaaattcctg actttaggat agttcttttc ttatttcagc
    19321 aattctgaaa ctggttcgaa aacaattttt tcaatgtcag aaaggtaaat ggccaattgt
    19381 tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt accaaagcct
    19441 tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag tttttgaatt
    19501 tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc tgctgcaatc
    19561 gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc gcgactgagt
    19621 tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttaatga cgactgtata
    19681 gtcggtattt tttgttatga gatgctcggc tctttccaag tcttgagcat ttttaaatga
    19741 aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga tgttaaccaa
    19801 ggaagtccca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat caggaacgtc
    19861 aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca tggtctggcg
    19921 ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct tactaatggc
    19981 ctgaccaatc gattccaaac gttccttgaa atcctcaatt ctatccagaa tggtctcgct
    20041 attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc gggtcatatc
    20101 tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat agaccgcagt
    20161 ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag aagtgacacg
    20221 atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa gcagatcctt
    20281 catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat aataggcatt
    20341 ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag ccatgggatg
    20401 ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat ccacaatggt
    20461 tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca aattggccaa
    20521 ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga catctgccaa
    20581 aggagcaaaa ctagcaaaat catccgttgc acggtcaatc atgccttctt tcaaggcgat
    20641 atctctcgaa gcttgactac gattataacc taaaatttca taatctggat gatcgcgttt
    20701 gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga ttgtttttgc
    20761 cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg cttcttttag
    20821 ttcctcaaga ttatctgatg agaatttttc gaggatctct tgcgccagaa ccgttgctac
    20881 aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc tctccacggt
    20941 tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat aaagagtagg
    21001 aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac caccttcaaa
    21061 accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga gaatttcatc
    21121 cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt ccaccccttt
    21181 aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc tatcccattg
    21241 gacataggaa ccaagaccaa ctggaactcc tccgacgact gtctccacaa ccccaccgat
    21301 ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt ctcgttcttg
    21361 gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga ctgtcagatt
    21421 ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa tctccatatc
    21481 cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg tggtttcacg
    21541 agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa tccccccaac
    21601 caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa ggcggtcttc
    21661 aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga tgacatccat
    21721 agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga caacctggtc
    21781 actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc taagatcctc
    21841 attgatatcc tcagctgtca atagaagtcc agctggaatt ccctcaataa tagctgttag
    21901 acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt attttaccaa
    21961 gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa gctctggcac
    22021 caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag cctgataaag
    22081 cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac acatctctgt
    22141 gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct tggaaatctg
    22201 taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg cagtcgcttc
    22261 gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat tgtccaactc
    22321 atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg tctctgcatg
    22381 ttccaaaata ctctcaacag aaccattcag tcccgtcaag agagcccaca gttctggatc
    22441 ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc tttttccgag
    22501 ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg tccccaccat
    22561 atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat caacctgagc
    22621 tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag aggctacaaa
    22681 tccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc tacgagtcag
    22741 accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct ttctttcttc
    22801 accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc tgagcttgac
    22861 cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct tttgcggttg
    22921 ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa tctgaatatc
    22981 ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct ttctttattg
    23041 gtatttttct gttaaagact gccaaatctc ttctgtcggc atttccttgc ctgtccacag
    23101 ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg ctggattgcc
    23161 ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga tatctgcaac
    23221 taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc catccatgcc
    23281 cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca gttcagaaac
    23341 atattctaaa gcacacaaat ccactttaaa acctgtctgc tcctgtaact tgtctaggta
    23401 aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac tgacgccatc
    23461 caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca gggtcatctt
    23521 tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat ctgtattata
    23581 tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca agtgcgcttc
    23641 atcgctcagc ttatccaaat aaggaatcac ctgctcctta tagggcatgg acagattgat
    23701 gccaaacatc tggtagcgac gaatattggc cactgtttct gccaagtcac tcgcttcaat
    23761 ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat tgtggatgaa
    23821 gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg tatagccatc
    23881 aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc tgcccagggg
    23941 cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc gcagtgatac
    24001 gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca ggattgaggg
    24061 ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg tgagccatca
    24121 ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt tccatcatgt
    24181 tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag tccagcattt
    24241 cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt tgatagagtt
    24301 gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt tctccacctt
    24361 cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca aaaatggctg
    24421 gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca atgatgtcgg
    24481 catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt ggcattactg
    24541 aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat tactactttc
    24601 atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa tctggtaact
    24661 tcttcctgca aaacctttat caatttgttc tgtaaatttc tgacgggaaa cattggcagc
    24721 attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct gggaaatcaa
    24781 cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa agctaggcgg
    24841 atctaggaca atcacatcgt aggtcaagtc tttgcgtttg gcatatttga aatactcaaa
    24901 gacatccatg actataaaac gatgctcgtc tgtgctgagc ccatttgcct gaaaatgcgc
    24961 ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc tagctcctcc
    25021 catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca aggatttacc
    25081 catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga aaattcctgt
    25141 catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa cagtgaaaaa
    25201 gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct taaagcggat
    25261 tttctcataa gctcctaaaa cctcagggaa aacctgtcta aaggcttctg atatagtctg
    25321 acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc cataaaggtc
    25381 cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg cagttgtcaa
    25441 atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg tttcaaagaa
    25501 agcttgattg aaggccacct tgtctttgct gataaaccag cccaagccct tgttttgctg
    25561 agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta cttcctgatc
    25621 cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct tagcaagctt
    25681 cttttcaacc cttttgctga ctcttattct attcataact accattatat caaactttta
    25741 gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa aaatggtata
    25801 ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacagtaaaa aatgctcttc
    25861 cgtcttggag gagcatttct ttttatcaac gaaaatcaaa tagcaaacta tgaaactagc
    25921 ctcaggttaa ccgtgagatt ataggtagag aggttgtatc agcaatatgt gtctgtcaaa
    25981 tttagtgaca aaggtagtag aagaaagatg aagaaataaa tcagcttcag tagatatctg
    26041 gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat caataaataa
    26101 tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat ttcgaactct
    26161 aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta acgtttttga
    26221 aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta tattatattt
    26281 cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt tacaactaca
    26341 ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca accaaccagc
    26401 tcatcttttt tctatttctg ccaatatacg tgacaggtaa taatgatagc caaaaatagc
    26461 aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga taggggagag
    26521 agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac tataaataaa
    26581 caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa tgctactcca
    26641 attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga tgacactggc
    26701 aaggatccat gccacaataa ggtaaatcat cgaaatgatg ggcaagccta gatatagaga
    26761 aagaccaagc aaagtcagaa ctggtaaaaa ggactggaca gcatatataa tccaaaattt
    26821 cactttcaca taacgagcaa agtcaaaggg taaactctta agaaaatcaa cattttccct
    26881 ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa ctgctataaa
    26941 cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa ctatctgaga
    27001 atctcggatt ttggaaatca gaccgatcat catgagataa ggaaagaaag cacttgtaaa
    27061 aagcactgta accacgccag taccctgtcc caagagggtg aggtggtagc gtaaaaccat
    27121 gcggaaaaat tcctttttag tggttgaaat tctctccttg ctgcgacgtt cttttttgac
    27181 cttctcctca ctattaagca ggatcacgtc ataaaaacga ggaaggacct tctttttggt
    27241 cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccaata tggcttctgt
    27301 cgaaaaagga tccactgcta ttttgtaaaa gatatgaaga ggataaagga gaaatggaat
    27361 gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga taaatattaa
    27421 aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat actttctgaa
    27481 gaccctagtt tgagccaaga aatgcactgc cactaccatc actagagcca cagagacaaa
    27541 taataaggtc aaggacagta gcatcaaagg caaacccagc catagagaag gagctagcct
    27601 aatgtagagg accagaaaat aagctaggat tggtacaatt ccagttagag ctggcaaaag
    27661 gacagacagt cctttagcaa ttataatctc tgattcttta aaggcatagg gcctatacga
    27721 tatcaaatcc ttactctcat aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc
    27781 aatcactagt aaaatagcaa tcatcaagct aaaataaata ggtatttcct caaaaggaaa
    27841 atgaatggct atattactaa aacagatgat catcaagaga ctggaaaaaa tgtaagaact
    27901 taagactcta gcggaaacat ttactttttt cccaggattc ttagcctgct tctttcgtag
    27961 attagccaga ttagcttctt gagatgaata aaggatattg atatcaacta attttttaat
    28021 gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg tagatacttt
    28081 ccaaagactg gtctgggtaa tcttttctca agtcctctac actaccacaa taaatcaaat
    28141 gccccttttt caaaatggca atccgatcac agacttgctc tgccacctct aggacatgag
    28201 ttgaaaacaa gactgtcttc cctttttgtg catgttcctt catcatctgt ttcaaatcaa
    28261 aggcagcctg gggatccaaa ccagtcaagg gttcatccaa gacccaaata tcgggatcag
    28321 acaagagtgc tccgatgaca aagactttct gacgcattcc gtgagaaaga gtttcaataa
    28381 cctgatagcg attttcagca aaatcaaaaa cgttcaatag cctagctaga ctagcctcca
    28441 agtcagatct actcagatca taggatgagg cgatcaattc ccaaaattca ttggccgtta
    28501 agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgttta atagccaagc
    28561 gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt gaaatgatac
    28621 tgactaggga ttttatagtg gtcgattttc cagccccatt atgaccaatc aagcccataa
    28681 tctctccatt ttcaatctgc aaattgagat tactcaaggc ctttttatca ccatacaact
    28741 tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta ttatatatcg
    28801 ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt aaaatagttt
    28861 ccttctatct tacgcaaacg tttgcgctaa tagatacttt atgatagaat aaagaacaag
    28921 attgacaagt aagaggaaac attatgcaaa atcaaacact catgcaatac tttgaatggt
    28981 atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca cacctagctc
    29041 atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac gaaaaagatg
    29101 tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa gggactgtcc
    29161 gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt aaagcgcagg
    29221 gaattcaacc tatggccgat gtggttctca accacaaggc tgctgctgat cacagggaag
    29281 cctttcaagt tattgaagtg gatcctgtag accgtacagt tgaacttgga gaacccttca
    29341 ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctac aatgacttcc
    29401 actggcactg gtaccacttc acaggtacag actatgatgc caaacgccgt aagtctggga
    29461 tttatctgat ccaaggggac aacaagggct gggctaacga ggaattagtc gataacgaaa
    29521 acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct gaagtcatcc
    29581 aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct ggtttccgtt
    29641 tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc gatatgaagg
    29701 aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac aaggaagcca
    29761 atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt cgtctccatc
    29821 agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc attttcacag
    29881 atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac cacgataccc
    29941 aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca gcctatgccc
    30001 tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac tatgggattt
    30061 caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta gccatccgaa
    30121 aagatttggc ctatggagaa caaaatgact actttgacca tgctaactgt atcggttggg
    30181 tacgttcagg tgctgaaaat caatccccaa gcgcagtcct tatctcaaat gaccaagaaa
    30241 acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta gatttacttg
    30301 gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc cctgtctcag
    30361 ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata accaagctag
    30421 gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg gatagatctt
    30481 tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta ggttggttgc
    30541 ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg attggtgcta
    30601 attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca aggacattga
    30661 ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt tgtttccagt
    30721 tatctgcaaa ggtacgaagg gcacctgcat cggatacaga cacttgacta gcaatgtaac
    30781 gatgaccgtt gacttcctta acatctttga agatatcgcc tgcggctgca gctgcggctt
    30841 tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga agtccttcta
    30901 ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca gctttaagag
    30961 catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc aagatacggc
    31021 gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc tcagaagtgt
    31081 tgccaacatg agtaccacca caaagttcaa tagagtagtc accaatagtc acgacacgaa
    31141 cttccttgcc gtatttctca ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa
    31201 tatccgtttc aactgtctta acttcaagag cttcccagat tttttcgttg acttgctgtt
    31261 caatcgcacg caattcttca gcagttacag cttggaagtg ggtaaagtca aagcgaagga
    31321 attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg
    31381 cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg cgattgctat
    31441 caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca actgtatgaa
    31501 gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaacc ttacctgact
    31561 catccaagat ttgtccgtgg tcagcgacct gtccacccat ttcagcatag aatggcgttt
    31621 ccgcaaagat aagtgaggta gttccttctg atacagcttc tacttctgca ttgtcagcaa
    31681 cgatagccac caatttagaa gacaattggc tagcattgtg gttgaagaca ctttctacag
    31741 tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca gctgacgcac
    31801 gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct acagtcatac
    31861 cagcctcttc agcaatttct tcagtcaatt caactgggaa tccgtatgta tcatagagtt
    31921 taaagacatc ttgaccagca atgacagatt gacctttttc tttcaagtct gccacaatgc
    31981 cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct tcgctcttaa
    32041 cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt tccatgattt
    32101 ttccaacagt tggaacgagt ttgtaaagga aaggctcgtt gatacccaat ttttgaccat
    32161 gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca tttcctggaa
    32221 gggcaccatc accgatagca aatgaaagag aacgaatgtg gtctgcgata accttgaagc
    32281 tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact tcacggataa
    32341 tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg gccaccaaac
    32401 gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat tcgctacgag
    32461 gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttcg atgtaacggt
    32521 cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca aaggcttccc
    32581 cacggtcaaa gaaaatctct gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt
    32641 tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag cggttgtaag
    32701 aatctttatc atctggatag taagtcatgt aaagtttctc agctgggaag tcaaaccatt
    32761 cagggcttgt caaaagctca taagcccaag tgatggcttc atcacggaag taatccccga
    32821 tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt
    32881 cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt tcagggataa
    32941 tggtcccgtc aaagtatttc ttgagggttg ctaccccaga gttgatccac aaaagagttg
    33001 ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg gtcgcccaga
    33061 aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg tctccttatt
    33121 tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg acacagaggg
    33181 aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta gaggtcagat
    33241 ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc aatttgaaat
    33301 tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca tacttatctc
    33361 gccaaaaggt caacttctta cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa
    33421 aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca ccagccgcat
    33481 catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga taggcaattt
    33541 ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa
    33601 gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa
    33661 aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta tgcaattgta
    33721 tgattgagta gcatgacttt ctagcttaga tggctcgcag caccgccatt tctctggact
    33781 aagacaagtg aaaattaatt ctcaactttc ttattataac gtttttttaa gcttgcgtca
    33841 actggaaatg atctccgttg aattagacca attccctaca tctctgatta ctttttcagg
    33901 atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt actaaacaca
    33961 gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga ctcagttgtt
    34021 aggtattgtc gatccaagcc atcctttaaa tggaatagta taacagtttg gttaacaatc
    34081 ataaaggttg gccagaaact ttttttgaaa aaagtagaca ttttcattat ttgttgccgc
    34141 tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc
    34201 tgtacttgag tacggcaagg caacgctgac atggtttgaa gagtataggc ttagtatact
    34261 actaggcaag caaataagca aataagcaaa taaacaacta gaatagaaaa agatagggct
    34321 ctaaaaactg acttctattc cttaaaaacg aaccagcttg actgattcgt cttcttacgt
    34381 ttatctccta cttccgatac attttaaact gtaggaagag gtcgctatat ttccctgtcc
    34441 atttatggtc aaatttctca taaacttcta ggtgtttcat ggtttcaaca tcgggataga
    34501 aggccttatc ttcctttgtt tcctctggga gcaattcctt cgctggtagg tttggtgttg
    34561 aatagccgac atactccgca ttttggagag cattttcagg tttcaacata aagttgataa
    34621 aggcataggc tgagttttgg tttttaactg ttttgggaat gaccatattg tcaaaccaaa
    34681 gattgctggc ctctgtcggt accacataac gtagattttc atttttttct aacatttggc
    34741 tggcttcacc agagaaggtc acgccgattg caacattatt ctgaatcata tagcccttca
    34801 tctcgtccgc aacgatagcc ttgatatttg gagtcagttt gtagagctta tccactgtct
    34861 cttccaactg ctgcagatcc ttggagttga ggctgtagcc gagggaattg agtcctagtc
    34921 ccagcacctc acgcgcccca tcaaagagca tgatagaatt cttatactcc ggcttccaaa
    34981 ggtcatccca atgctcaggc gcttcatcta ccatggtttc gttgtagaca attcctaagg
    35041 ttccccagaa gtaagggatg gagaatttat tacctgggtc aaaggactgg ttgagaaact
    35101 ctgatccgat attttcgatt ccttcaattt ttgaataatc aagcggaacc aagaggtctt
    35161 cgtccttcat cttgttaatc atgtattcac ttggaatggc aatatcgtag gtcgttccac
    35221 cctgctttat cttagtgtac atggcttcgt tggagtcaaa agtctcgtac tgaacttgaa
    35281 ttcctgtttc ttctgtaaac tgagtcaaga gttcaggatc gatatagtct ccccagttat
    35341 agataaccaa tttttgacta tctcgactat tgattttact atctaaatga gtcgcaattc
    35401 cccacaagac aaggataatc gctgcaattc ctgctaaaaa tgaatagatt tttttcatgc
    35461 ttgctcctcc ttctcacgag agataaagta ataacctaca actaggataa tactaaagag
    35521 aaagactaga gcagacaggg cattgatttc taaggaaatc cccttgcgag cacgagagta
    35581 aatctcgact gatagggttg aaaagccatt tcctgttaca aagaaggtca cggcaaagtc
    35641 atctaacgaa taggtgaagg ccatgaaata accagtaatg atagacggag tcaggtaagg
    35701 aagcatgatt tccttgaaca tctgaaattg actagctccc aagtcatagg ccgcatgaat
    35761 catgtcgcca ttcatttcct tgagtcgagg caagaccatc aagaccacga taggaatgga
    35821 gaaggccacg tgactagata gaacggtcaa aaagccaagt gaaaacttga gttgggtaaa
    35881 gagaatcaag aagctagcac caatcataac gtcaggcgca accatgagga tattattgag
    35941 tgatagaaag gcttcttggt atttcttacg agactggtag atgtaaatgg caccaaaagt
    36001 cccgataatg gtcgctatca aggctgatag gaaggccaag aaaaatgtct gagccaaaat
    36061 cagcatgagt ctcccatctc caaacatggt ttcaaagtga gtccagctaa aacctgtaaa
    36121 gctattcata tcatcaccag cattaaaggc atagccaatc aagtaaaaga taggcaggta
    36181 gaggaccaga aagaccagtc ccagataaag gttggcaaat tttttcatcg ttctctcctt
    36241 tccttagtca cccacatggt gatgaacatg gtcaggatga gaatcacacc gatggttgaa
    36301 cccataccat agttgtcatt ggttagaaaa ttctgctcaa tagccgtccc caaggtgata
    36361 acgcggttcc caccaatcaa acgggtcagc atgaagagac tcaaacttgg gataaagacc
    36421 gactgaaccc cacttcgcac accgttcata gatagaggga agatgacatg acggaaggtc
    36481 tcccacttag ttgcaccaag gtcataacta gcattgatga gattattatc catatcgtct
    36541 aagacattga aaatcggcaa aatcataaag gggagctcga tgtagcttgc gacaaagata
    36601 aaggaaaaat cggtaaaaag caactgttgt gaaccaattc cgataaattc caagaattgg
    36661 ttaatagagc cattttgacc aaaaatcccg ataaaagcat aggctttaag gagcaaattg
    36721 atccaggtag gaaggataat cagcatgagc cagagttgac ggtgcttgag acgggtcaaa
    36781 aagagggctg ttggatagct gataagcaag gtcacaaagg tcacaatccc tgcataaagc
    36841 actgagttga aactcatttt gaggtaggtc aagttttgtg acgcaaagta agatttataa
    36901 ttttctaaac taaactggcc ttcaatgttg aaaaaggatt gtccgaaaat caagaccaag
    36961 ggtgccaata caaagagggc aatccaaagc atgtagggca ctacaaagag tttagagctt
    37021 gttttcttca tctctttcct cctcgatcgc attgatcaga cctgcttctt gctcttcgat
    37081 ttccacgtat tcttcgatac gagcatcgaa ctcttcttcg gtttcattga gacgcatgat
    37141 gtggatgtct tctgattcaa agtccagacc gatttcctca cccacgatgg ccttacgggt
    37201 tgagtggatc atccattcat ttccaagttc gtcataggcg ataatttcat aatgaactcc
    37261 acggaagagc tgggtgtcga ccttaacttg gagtttgcct tcttcaggaa gggtaatgcg
    37321 caagtcctct ggacgaataa cgacctcaac aggttcattt ggcttcatcc caccatcaac
    37381 cgcttcaaag cgtttgccgt taaattcgac caagtagtcc tcaatcatgg tacctggcaa
    37441 gatgtttgac tccccgataa aggtggcaac aaagtggttg attggctcat cgtagatgtc
    37501 cacaggggtt ccagactgga caatctcgcc atcattcata acgaaaatcc agtcactcat
    37561 ggcaagagct tcttcctgat cgtgagtgac aaagacaaag gtaatgccca atcgttgttg
    37621 taattcacgc aattcgtact gcatgtctgt tctcaatttc aagtccagcg ctgataaagg
    37681 ctcgtccaac aagaccacac ggggttggtt gatgatagca cgggcgatgg ccacacgctg
    37741 acgttgtcct ccagaaagtt tgcggatgga acgtttttca taaccttcca actgaaccat
    37801 cttgagaact tccgctacgc gttgctcgat ttctttcttg tcaatcttgc gcaagcgaag
    37861 tggaaaggca acattttcaa acacattcat atgtgggaac aaggcatagg attggaagac
    37921 agtatgtacg tcgcgcttgt tggttggaat atcattgata cgaacaccgt ctagcatgat
    37981 atctcctgtc gtcgcatcca gtaaacctgc aataatgttt aggatagttg atttccccga
    38041 accagatgca cctagaaggg tgtagaattt cccttcttcc aactcaaagt tgatgtcttt
    38101 gagaaccttg gtgttgctgt cttcaaaaac tttagagacg tttttgaatt cgataattgg
    38161 ttttttcaat tggcataaat tccttctttt tcatagatta accgatcggg gctctgtcag
    38221 gtccccacta cctcttgcag ggagtaaaac cacctgcata catcttcgct accgataggc
    38281 tttcaccccc ctttccatga catagcagca gcttttcaaa tacagcttcc tacttgcttt
    38341 cacccaagat ccggacttct ctttcaagcg taatacctga gtgttccttg actttttcga
    38401 taaccgattg gatcaagtcc tcgtagtctt tggccgttcc atctgcgaca ttgatcataa
    38461 atcctgcatg cttttctgac acttctacgc caccgatacg atagcctttc aagccagctt
    38521 ctgaaattaa ctgacctgca aaatgcccga ctggacgctt aaagaccgag ccacaagatg
    38581 ggtattccaa aggttgcttg agttcacgta ggtgcgtcaa gcggtccatt tcctgcttga
    38641 taacctgatg ggttcctggg gctagggcaa atttaactga caagacaact gcaccagact
    38701 cctgaatagc tgaatggcgg taaccaaaag ccaagtcttt agcagacagg gtttcgattt
    38761 ctccatcctt ggtcaagacc ttacaagact gcaatatgtg agcaatctcg ccaccatagg
    38821 cacccgcatt cataaagaca gcaccgccaa cgcttcctgg aataccacaa gcaaactcaa
    38881 agccagtcaa actatgacgg agggcaatgc gagttgtttc aatcaagtta gccccagctt
    38941 ctgcttcaat ggtatagcca tcaacagaaa cgttattgag cttgtcacac aagatgacaa
    39001 atccacgaat cccaccatca cgaacgatga tattgcttgc attgccaaga accatccaag
    39061 ggatattttc ttggttggca aatttcacaa ggcgagccaa ctcaaaacga tttcgtggaa
    39121 agaccaaata atcagcctct ccacctactt ttgtataact atagctatgc aagggttcct
    39181 gaaaacggat atcaattcct tctaagattt caagcatttt ttctcttaca gacatgtcac
    39241 tcttcctttt acaaaattca ttccattata ccatttttag agacatttga cgaccataaa
    39301 aataccttgt ttggattttg cataagaaaa agaggttccc ctctttttat gattttttac
    39361 aaaagatttc cttggttcca taggcgacca gaacgagctc cagtgctaga atcacttcaa
    39421 ccaagactgg atttgtcaac cagcctactt ggaaaagaga tggtgccaga tcaaagaagg
    39481 catgcaagcc ataggctgtt aggagataaa tccatttctt ctggcgaaca gcttgataaa
    39541 cccaaactgt caaaagtaat tggaaaccaa gcgccaagat tcgctcaaaa ccaagcaaat
    39601 aaatctgcca gaccgaaagt gactgaatgg tttttaacat attttcagac agtaattgca
    39661 taacctgtgg attctgagtt tgaactgccg aaagaacaat gtaaagattg agtaaactag
    39721 taaggcctag aaaaatcaac tccaagccac catgccccaa tccataggcc aaggcgtctg
    39781 ccttttccaa acttctcttt ttctccaacc atttgaagaa aacaagacga gccgtttcct
    39841 caaaaaatgc taccatggct aggccataga taatatagat aagcggatga acttacaaga
    39901 gggcaatact accgtctttt tgaggatgca aaatcaagat atgcaccagt ttttccaaaa
    39961 tctgcgaaga gacaaagaaa gcgactgcac ccaagtccaa gacagctaga ttaatctggt
    40021 atttcttttt ggcaaaccaa atgcttccta tcagaaaagc tagcaacagc aacatggtaa
    40081 taatgatatg aatggtcatt ttcttctcct attctgcctt ttcaatatct tttttcatct
    40141 cgtcaacatt gaacttagca aacaagtatt gacggtcttg gactgggaaa cgttggtcca
    40201 actggtcaac tgctcccacc tcgataatgc cttccttgat gacaaaatcc atgacatgtc
    40261 caccgaaggt caaatcatct gatatgaagt gcagatggta gcctgccaca ctgaccccat
    40321 ggaaaatctc aggcgtccag aaaccaacaa tagtccccac aacattgtca cgactatatt
    40381 ccggttgatg ggttgcgaca tcagcaaact tggtatcggg tgttgactta ggaatcatac
    40441 gcacatgcat atgtgaaaat tccccccgaa tcttgataga gcggaaaaga ttttccccat
    40501 cataataaga ctcaattcgt tcttccaatt ccttgtctgt catctcaaag cgctggcgga
    40561 aaatgacctc tgcctgatgc ggtactactg ctgcataagg aataagggca tctggtgaca
    40621 cttccacaat ttctggctgg tctcctgacc ctttggcctg ataagccttg ccatccaaga
    40681 caatcaattc cccatcaata gaatccaagg ttcccaaacc aaggtcacca tgctctagca
    40741 attctcccac tgtcatggta ccaccataaa ggccagccat caaggctcca agagtattgt
    40801 attgaaataa tttcactggt tcctgcacgt tcttatccat tcactttctt gtctgttatt
    40861 ctataaatct tactcctaag tataccacat ttgcccctag atgtgaacga gagaaacgct
    40921 ctagacattg ccaagaagga aaaaaaaggg tacaatgtaa caaaatcaag ggaggtctgg
    40981 aatgaagaaa caaagcaagt acaaagaggt cgtttcctat ctgaaaaatg gtatcgagtc
    41041 tggacgattt ccgacgggta gtcgcctgcc ttctatccgt caactgagcc ttgactttca
    41101 ctgcagcaag gacaccattc aacgagccct gctggaatta cggcacgaac aatatctcta
    41161 tgccaagcct cagagtggct actatgtatt agaacaaggg caacatcaag acctagaaat
    41221 cgaggttacc gacgaacatg ccagtgccta tgacgatttc cgactctgtg tcaatgaaac
    41281 cttgattggc cgagaaaact acctcttcaa ctactatgac aatcaagaag gattagaaga
    41341 cctaagacag tccattcaca aactcctctt tgagcaagct ctctactgca aggctaacca
    41401 actagtactg acttctggaa cccaacaagc cttgtttatc ctctctcaaa tatcctttcc
    41461 tagacaagcc aaggaaatct tggtggaaca gccaacctac catcggatga atcgcctctt
    41521 gattgcacag gggctggact atcaaacgat tgaacgaggc attgatggga ttgacttgga
    41581 ggagctggaa ggccacttca aaacaggaaa aattaaattt ttctacacca ttcctagatt
    41641 tcactatcct ctgggacatt cctattctga gcaggacaaa cgatctattc ttaacttagc
    41701 tgccaagtat gatgtctata tcgtagagga cgattatctg ggtgatttgg actccaagaa
    41761 gggccaaacc ttccactatc ttgatacaga ggagcgtgtc atttatatca agtccttctc
    41821 gaccagcctt tttcctgccc ttcgtattac agcactcatt cttccaaatg ctatcaaaga
    41881 agcatttgtg gcctacaaaa atatcctaga ctacgacagc aacctcatta tgcaaaaggc
    41941 cctgtcactc tatattgaca gtcaattgtt tgaaaaaaat cgtttggctc gcttgaccaa
    42001 tcatgaatct taccaaaaac aaatcgagga aaggataact aaaacacctt gtccccttcc
    42061 tcattatccc ctacacgatg gtttattgct agacctgaga cagtatccta aaatcgccag
    42121 tctcaaacac agtcaactgg gcttggactt ctttgaagag gcctatttaa gcacctgtcc
    42181 ttatcaattt gccaaggtgt ccctagacaa tctggaaaat gttttaaact atttaaaagc
    42241 agaattggaa tgacatccaa ctctgctttt tcttattctt caacttcagt taaacttgct
    42301 gctttttcaa gatagctttg gtaagttcct tcatccttaa gtttttggat gaccttgtct
    42361 accacttctt tcaaatcatc actatttttt ctaagagcaa cggcattggc gtcgccgtcc
    42421 ttcatcttca agctgacagt tgcgacagct aagccagcgt ttttagcagc ataactaagt
    42481 gcaacaggct catccatatg aacagcatct acttttccag cctgcaattc attgactgct
    42541 tcacccatat tagttaggga agttaattga gcttttggca attgttcctt gaccattgat
    42601 tctggaacag tccctttttg ggctgcaata ttagcacttt ctaggctagt taaatccttg
    42661 tatttttcca catcagcctt acgaaccaag aaactaatct tgttttcata gtatgggatt
    42721 gaaaaatcaa agacttcttt tctctcgtca gtagcactaa ttcctgcaac tgctaggtca
    42781 gccttaccag tttgaagact ggtcaaaaca ttatcaaaac tcatgcttga gatttccaac
    42841 ttaaccccaa gttcatcagc gatagcctga gccatgtcga tgtctgcacc gactacctgg
    42901 ttctttccat caaccaatga ttgaaattca aagggtgcat agtcaggact ggttgccaca
    42961 actaatttcc ccttttgctt aatggcctca acagctgact gagaaccatt agaacctgac
    43021 tggcaagcta ctaagaagaa acttgcaaga aaattacata aaacaaatat ccattttttt
    43081 gatttcataa ataaacaacc tctctgttaa ttttgtataa ttataacgat atccaagtta
    43141 cttgtcaagt gttttttaaa tttttatctc aaaaatattt tttcgttcaa aaaaaggagc
    43201 catcagttga tttcaagctc ctttttatac agaattaaac tattttatag ttcgacaatc
    43261 ttacctgttt caaagtagac aacccattca cagatatttt tagcatagtc accgatacgc
    43321 tccaagtagg aaataacttg gaaataatca cgacccgtaa caatggcttc tggatttttc
    43381 ttaatctctt cagtcgcaag gtcacggata gtttcaaaat agtggttaat ttgctcatcc
    43441 atggaggcca cccggtatgc gtcgtcaaca gaaccattaa gataaagatc aagtgctgct
    43501 tcaacaacgc ttttaacttc acgtcccatt tttttaattt cttcctctac agctggaatg
    43561 cgctcttccc ccttcatacg gatggttgcc tgagcaatgg ctacagcgtg atcccccata
    43621 cgctccacat ctgatacagc cttaaggaca gtcaagactg tacgcaaatc ttgagagact
    43681 ggttgttgga gtgcgatcat ttcaaatgat ttcttttcca gtttcacttc gtattcattt
    43741 acttctgcat catcttcgat gacctctttt gccaggtcac ggtcatgcgt gacaaaagca
    43801 cgtaccgtac gattgatttg tgagagcact tcttgtccca tagcgtagaa ctggttatgt
    43861 aatttctcta aatcttcttc aaattgagat cgtaacatct ttcatctcct tatccaaatt
    43921 ttcctgtaat atagtcttcc gtttccttgt gttggggatt aaggaacatc tgcttggtat
    43981 cattaaattc aatcaaatct ccatctagga aaaatcctgt cttatcagag atacgtgaag
    44041 cttgctgcat ggaacgggtt accagaagca tggtgtactt gtcttttaga ccatacaagg
    44101 tttcctcaat tttaccagct gaaatcggat ccaaagccga agttggctca tccaagagga
    44161 tgattttagg actagttgcc aagacacggg ccacgcagac acgctgctgt tgaccacctg
    44221 acaatccaat agctgaatca tatagacgat ccttgacctc atcccagata gaggcacctt
    44281 gcaaggcttt ttctacggct tcatccagaa cctgcttatc cttaattcca ttgatacgaa
    44341 gcccgtagac aacattctca tagatagtca tagggaaagg attaggttgt tggaaaacca
    44401 ttccgatttc cttacgtaat tcaaccgtat ctgtacgcgg actgtagatg ttgtgaccat
    44461 tgtacaccac ggatccagtt gtggtcacct ctggattgag atctcccatg cggttgagag
    44521 acttgaggag ggttgacttc cctgatccag atggaccaat caaggctgta atttccttag
    44581 gttggaaaga tagggaaaca ctattcaaag ccttcttttt attataataa acggacaggt
    44641 ctgatacctg taaaatcgca tctgtcatac ggtttccttt ctaaccaaag tgaccagata
    44701 catagtcatt ggtggactgt agcttggcat tttggaaaat agttgcagtc ttgtcatact
    44761 caatcaaatc acccaagtaa aagaagcctg tatagtcact tgcacgagca gcctgctgca
    44821 tattatgcgt tacaatgatg atggtaaagt ttttcttgag ctcaaacatg gtctcttcta
    44881 gttgcatggt cgcaatcgga tccaaggctg aggctggctc atccattaag aggatatctg
    44941 gcttaacaga gatggcacga gcgatacaga gacgttgttg ctgaccacct gataaggtca
    45001 aggctgactt gtggagatcg tctttaacct gatcccaaag ggcagcctga cgaagggagg
    45061 tttctacgat ttcatctagg gcttgcttat ccttaactcc agcacgttca tgcgcaaagg
    45121 taatattacg gtaaattgac ttagcaaatg gattggggcg ttgaaaaacc attccaatgt
    45181 gtttacgcat ttcataaacg ttgatttctg gacggttgac atcaattcca cgatagagaa
    45241 tctgcccagt tactttagca atatcaatgg tatcattcat gcgattgaga ctgcgtaagt
    45301 aggtagattt ccccgatccc gacggaccaa tcaaagctgt aattttattt ctttcaaatt
    45361 gcatatcaat ccccttaatg gattcatttt taccatagta aacatggaca tccttagtag
    45421 aaagggctac tttttcttca ggaaaggtaa ggatatgctt ctcatcccag ttatatgttg
    45481 acatggcttc tcctttaggc agcggttaat ttcttgtgta gatagcttcc gaacttacga
    45541 gctccaaagt taaaaatcag gataaagatc aggagcacag cggcagaacc tgctgataca
    45601 atggttccat ctggaatagt gccttcacta ttgactttcc agatatggac agccaaggtt
    45661 tctgcttgac ggaagataga gatggggcta gtcacactga ggatattcca gttagaccag
    45721 tcaagagctg gcgccgattg ccctgctgta tagatcagag ctgcagcttc gccaaagata
    45781 cgaccagatg ccaaaacgac acccgttaca atacctggaa gcgcttctgg aataacaaca
    45841 tgaaccactg tctcccagcg agaaatccca agagccagac cagcctcacg ttgggtatgg
    45901 tgaacgtgtt tcaaactatc ctctacatta cgcgtcatct gaggcaagtt aaagactgtc
    45961 aaggccaagg cacctgaaat gattgaaaat ccatactcaa actggactac aaagatcaag
    46021 taaccaaaga gacccaccac cactgatggt aaagaggaca agatttcaat acaagtccgc
    46081 acaaagttgg taacaggacc ttttttagca tattcagcca agtaaatccc agctcccata
    46141 gaaagaggta cagaaataat caaggtaatg accaatagga aaaaggaatt gtaaagctga
    46201 atgccaatcc caccacctgc ttgaaaagca gaagaccttc cagtcaagaa agaccaagag
    46261 atatggggca agccccgaac caagatatag agaatcaagg aagccaagat tgtcacaatg
    46321 atgctagcaa tcgtatagag gacagctgtt gcaagtttat ctaatttctt agcgcgcata
    46381 atttttcttt cctctttctt tcgtaatcaa tttaatcaca ctgttaaaaa ctaaactcat
    46441 caagagcagt accaaggcca gtgaccagag aacattatta tttacagttc ccatgacagt
    46501 gttcccaatt cccatagtta atatagaagt taaagttgca gctggtgtgg tcaaggaagt
    46561 tgggataaca gctgagtttc cgacaaccat ctggatagct agagcctcac caaaggcacg
    46621 cgccatccca aagaccactg cagtgaaaat accagaacgg gccgccttca agatcacacg
    46681 ccagatagtc tgccagcgag tggctcccat agcgaaactg gcttcacgat aataacgagg
    46741 aaccgcacgc aagctatccg ttgtcataaa ggttacggtc ggcaaaatca tgacaaagag
    46801 gacggaaatc cctgacaaaa tcccaaaacc agtcccacca aagacactgc gaacaaaggg
    46861 aacgacgact tgcaagccaa taaatccgta cactactgaa ggaatcccaa ccaggagttc
    46921 aatagctggt tgcaaaatct tcgccccttt tggtgatact tcggtcataa aaactgctgc
    46981 accaatagca aagggtgttg cgataagggc tgagagaatg gtaacgataa aggaacccaa
    47041 aatcatagga agggcaccaa attctttact agaaggattc caagttcctc ccaaaagaaa
    47101 gtcaaagata ttcacaccat tgacaaagaa ggtcgacaag cctttttgcg ctacgaaaac
    47161 caaaatcatg gccacaagga tgactatcaa agaaagacag gcaaaggtca aaccttttcc
    47221 taatttctcc agacgagaat tctttgatgg aagcaacatt ttcttagcta attcttcttg
    47281 attcattatt gtctcccttc caacactgtc acagttccgg cagcatcttt ttcaaccttc
    47341 atttccttaa tcggaatata cttcaatcct ttgacaatcc cttcttgggt ctcatccgag
    47401 agaacaaaat tgagaaattc tgcagccaac tcattgggct gccccaatgt atacatatgc
    47461 tcataagacc acaagggcca attattgcta cttatatttt ctggacttaa gtcatagcca
    47521 ttcaacttca tgcttttgac cgaatcatct atataggtaa gagataaata agagatagct
    47581 cctggacttt ttgatacgat tgattttacc gctccatttg aatcctgctc ctgactttgc
    47641 atggcagact gaccttccat aatgacagta tcaaaggtag cacgagagcc agagccggct
    47701 gcccgattga taacagagat gggtaagtcc ttaccaccaa cctctttcca attggttacc
    47761 tcacctatga agatttgacg aagttgctct gtcgttaggt tatcaacatc aacctcctta
    47821 ttgacaatca gagccaagcc agctaccgcg accttgtggt caacaagagc agaagcatca
    47881 attccgtctt tttcctcagc aaatacatct gagtttccta tatcaactgc cccagactga
    47941 acctgggaca agcctgtacc agaacctccc ccttggacat tgaccgtttt tccaacatgg
    48001 atcgtgccaa attcatctgc cgctacttca accaagggtt gcaaggcagt tgagccaaca
    48061 gccgttatgg attctccacg atcaatccag ctagcacagc ctactaaaca agccgtcagc
    48121 caaaaagcga taagagacag agcaagcttt tttctttttt tcactgtttt tctcctcgaa
    48181 aataattatg aatactgtga attttttaag tagttcttta tgagttgacg catgaattct
    48241 taccaaattt ctgcgcaatt gattatttat ataatatagg ctatattact ctttcctaac
    48301 ctcctttttt catatgtgga taaaatctct tgtctatccc ttcccccatt gtcacccatt
    48361 atagtcattt cgtgtctctt tttccccttt ttaatgcaag ggaaattact cttcttagat
    48421 gataatccaa aagctagaaa ggtatctcaa acctctctac tctcccagac tagtttacaa
    48481 ctaaaaggaa aagattctat tttatgagaa atctagttta caagcggtaa gaacgctaat
    48541 aactaaactt cttgtactct ttgaaaatct cttcaaagca gtgttttgag ctatctatgg
    48601 ctagcttcct agtttgctct ttgattttca tcgagtagta aaactacatg tgatggcaat
    48661 caagatatca agaatcatcc tactaaaaaa atccatactt tcactataac atagaataag
    48721 atatttgact agcattttca tttgaatctg aggccttttg gaaaataatt tttcaaaaca
    48781 tttccagtaa cctttgcaaa gcccaagcca ttgcctttaa ccaaaacttg gtaccaacca
    48841 tttggcagac tttctgccag ctgaacggtt tctccagccg catacttgac aaacgcttct
    48901 tggccaattt caaccgactg ttcgacctga ctcggtttca aggctaaacc aagagcgaaa
    48961 ctgggctcaa agcgtttctt cttaaaagta cccagatgca gtccattgcg agcaatcttg
    49021 agcttcccta aatctggcaa aagttctggc aagagataaa gctggtctcc aaaaatctgc
    49081 aagatacccg gtagattgac cttcaaatgg ttttgggcaa attcctgcca caaggcaact
    49141 tgttcacggc tgaggttact cttacttgcc ttaaatttag gagctggatt gttaccctta
    49201 aactgtagat gggcaacaaa ctgaccctct cccttaaact gatgagggta catccgagcc
    49261 gtttctggca ggtcaatacc agctaccatt ccattgatat gctctactgg caacaagtca
    49321 aaatcatact cttccagcaa ccaattgaca atctcttcgt tttcctcggg tgcccaggta
    49381 caggtcgaat aaaccagacg accaccttca gctaacatgg tcactgcatc ctccagaatt
    49441 tctctttgca agctagcaca ttgactcgga taatctaagc tccaatagtc catagcatca
    49501 ggttgcttac gaaacattcc ttcaccagag caaggggcat caagaacgat taagtcaaaa
    49561 tagcctttaa agaccttgac caagcggtcg gcagattcat tggtcaccac gacatttgtc
    49621 gctccaaaac gctccatgtt ttcaaccaaa atcttagccc gtttgcttga aatttcattg
    49681 gaaacaagta gcccctcccc tgctagatag gctgccagtt gagttgattt gccccccggt
    49741 gcagcagcca agtccaagac cttcatacca ggactgggtt gggctacttg agccaccatt
    49801 tgagcagcag gttcttgcga ataaactaaa cctgtagcat gctcaggcga tttccctgaa
    49861 accttcccat agtggcccca aggggtttga gtaatggcat cagaaaagga aagttgctct
    49921 tcttttaagg gattgacccg aaaggccgaa accgcttcct cctcaaaaga ggcaagaaaa
    49981 tctcttgcct catctcctag tatttcttta tatttttcaa caaatccttc tggaaatttc
    50041 atttaagttc ttttcctttc gtaaatatag gactgaattt cctcctgcat ctcaagaggc
    50101 accatcatga ccggctgtct ggtttgaaaa tcaggagctt caccaaaaag ggtcacaacc
    50161 cgatagccca gactttcccc taaaatacta gctgcggcat aatcccatgg ttgcagatag
    50221 gtgagatagg tcaacaaatg ccctgacaaa atcttggcaa aactaatggc cgcacttcca
    50281 tagacacgaa caccaagaac cgctcggctc aaatcagcca gcccccattc attggtttcc
    50341 agcataccac tattccctgc aatgagaaaa tctccaagtg gtttagtttt aaaaggagct
    50401 aggggcctac catttagaca aactggaaat tccccaccac cgtggtaaca atcccctttg
    50461 accacatcat aaatcagacc aaactgtccc tgaccatttt caaaataagc catcataaca
    50521 gcaaaatctt cctgctgggc tacaaaatta ttggtaccat caatgggatc aatgacccaa
    50581 accttgccct cttgaaccga ggctcgcaga caaccttctt cagcacaaat cttatcctca
    50641 ggataacggg acaaaatctc accaaccaag agttcctgaa cttctttgtc cagtctggtc
    50701 accaaatctg ttggagagga cttggtttca acacgcaagt cttcctgcat atggtcaaga
    50761 atgtactggc ctgctttctt aacaagctct ttagcaaatt caaatttact ttccaagaga
    50821 aatctttcct tccccttttt ctttggcagc ctgaactgct cggtaaagtg aatagccact
    50881 aaccgtttca aattctcgtc ccaaacgttt ctcttcgctc ttgcttggca cgaccgcttt
    50941 gaatccctta taggagtcca gtaacttttt agcctctact ttgccttcat aggcagcttc
    51001 aacatcatta aaaaaagaaa gcactgaagc aagttcttca gtgctccacg acaaatctag
    51061 tgggtaacta tactgtttgt tcattaacta ataccagctc tcattcttgc ttcttttagt
    51121 tcttgcttac gataactacg agggagaaaa gcacgaatct catcttcatt aaaaccgatt
    51181 tgcatacgct tggcatcaat aataattgga cgacgcaaaa gactaggata ctgctcaatc
    51241 aaatgaagca attccgatac cgaaatactc tctacatcaa tattcaattt ttgaaaaatt
    51301 tttgaacgag ttgaaatgat gtcatcagta ccattttcgg tcaaggaaag gatgtgttgc
    51361 aattcttttc ttgttaaagg actggtcata atattgtgtt ccacaaaggg aaccttatgt
    51421 ttttctaacc aggccttagc cttacgacat gatgtacagc tcggtgatag aaatagtgta
    51481 atcatgcttt tctcttctta tctatacttt gctacttcta ttatacaaaa aaataaagcg
    51541 cttgactagg gatttttaga aaaaaagcct attttttcaa gaaaaatagg c
//
LOCUS       DAVKJU010000003        49855 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVKJU010000003 DAVKJU010000000
VERSION     DAVKJU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.003"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..766
                     /locus_tag="VW191_000127"
     CDS             <1..766
                     /locus_tag="VW191_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9622978.1"
                     /translation="NTKSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVT
                     IKKGETVAIVGESGSGKSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVL
                     QNSTLSYGTLRENLTFGHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSG
                     GQQQMILLARCLLSKPSVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIK
                     SADKILVMHNGEIVEIGTHRELLELGGIYKQLYSNN"
     gene            790..2529
                     /locus_tag="VW191_000128"
     CDS             790..2529
                     /locus_tag="VW191_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9622979.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            2580..3347
                     /locus_tag="VW191_000129"
     CDS             2580..3347
                     /locus_tag="VW191_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9622980.1"
                     /translation="MSQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRISSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     IGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            3349..3981
                     /locus_tag="VW191_000130"
     CDS             3349..3981
                     /locus_tag="VW191_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9622981.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLVYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4927..5340)
                     /gene="ndk"
                     /locus_tag="VW191_000131"
     CDS             complement(4927..5340)
                     /gene="ndk"
                     /locus_tag="VW191_000131"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU9622982.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(5451..9128)
                     /gene="rpoC"
                     /locus_tag="VW191_000132"
     CDS             complement(5451..9128)
                     /gene="rpoC"
                     /locus_tag="VW191_000132"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU9622983.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGAIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(9161..12772)
                     /gene="rpoB"
                     /locus_tag="VW191_000133"
     CDS             complement(9161..12772)
                     /gene="rpoB"
                     /locus_tag="VW191_000133"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9622984.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            13188..13258
                     /locus_tag="VW191_000134"
     tRNA            13188..13258
                     /locus_tag="VW191_000134"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13220..13222,aa:Cys,seq:gca)
     gene            complement(13456..14787)
                     /locus_tag="VW191_000135"
     CDS             complement(13456..14787)
                     /locus_tag="VW191_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEU9622985.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(15016..15840)
                     /gene="endA"
                     /locus_tag="VW191_000136"
     CDS             complement(15016..15840)
                     /gene="endA"
                     /locus_tag="VW191_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEU9622986.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15879..16067)
                     /locus_tag="VW191_000137"
     CDS             complement(15879..16067)
                     /locus_tag="VW191_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU9622987.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(16060..17343)
                     /gene="murA"
                     /locus_tag="VW191_000138"
     CDS             complement(16060..17343)
                     /gene="murA"
                     /locus_tag="VW191_000138"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9622988.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(17367..17597)
                     /locus_tag="VW191_000139"
     CDS             complement(17367..17597)
                     /locus_tag="VW191_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEU9622989.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17655..18692)
                     /locus_tag="VW191_000140"
     CDS             complement(17655..18692)
                     /locus_tag="VW191_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU9622990.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18676..19164)
                     /gene="coaD"
                     /locus_tag="VW191_000141"
     CDS             complement(18676..19164)
                     /gene="coaD"
                     /locus_tag="VW191_000141"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU9622991.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(19154..19693)
                     /gene="rsmD"
                     /locus_tag="VW191_000142"
     CDS             complement(19154..19693)
                     /gene="rsmD"
                     /locus_tag="VW191_000142"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU9622992.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19758..20750)
                     /locus_tag="VW191_000143"
     CDS             complement(19758..20750)
                     /locus_tag="VW191_000143"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEU9622993.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTSKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20862..21545)
                     /locus_tag="VW191_000144"
     CDS             complement(20862..21545)
                     /locus_tag="VW191_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEU9622994.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(21601..22341)
                     /locus_tag="VW191_000145"
     CDS             complement(21601..22341)
                     /locus_tag="VW191_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU9622995.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRDQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSPALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22375..22653
                     /locus_tag="VW191_000146"
     CDS             22375..22653
                     /locus_tag="VW191_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU9622996.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            22731..23657
                     /gene="yidC"
                     /locus_tag="VW191_000147"
     CDS             22731..23657
                     /gene="yidC"
                     /locus_tag="VW191_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9622997.1"
                     /translation="MKSIKRFALSAMGAAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVHEELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23732..24526)
                     /gene="pflA"
                     /locus_tag="VW191_000148"
     CDS             complement(23732..24526)
                     /gene="pflA"
                     /locus_tag="VW191_000148"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEU9622998.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(24652..25902)
                     /locus_tag="VW191_000149"
     CDS             complement(24652..25902)
                     /locus_tag="VW191_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU9622999.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVASIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25969..26796)
                     /gene="purR"
                     /locus_tag="VW191_000150"
     CDS             complement(25969..26796)
                     /gene="purR"
                     /locus_tag="VW191_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEU9623000.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(27063..28004)
                     /locus_tag="VW191_000151"
     CDS             complement(27063..28004)
                     /locus_tag="VW191_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEU9623001.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            complement(27994..29250)
                     /locus_tag="VW191_000152"
     CDS             complement(27994..29250)
                     /locus_tag="VW191_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU9623002.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(29252..29914)
                     /locus_tag="VW191_000153"
     CDS             complement(29252..29914)
                     /locus_tag="VW191_000153"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEU9623003.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29877..30533)
                     /locus_tag="VW191_000154"
     CDS             complement(29877..30533)
                     /locus_tag="VW191_000154"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9623004.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30544..31422)
                     /gene="rsgA"
                     /locus_tag="VW191_000155"
     CDS             complement(30544..31422)
                     /gene="rsgA"
                     /locus_tag="VW191_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU9623005.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHIPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(31424..32296)
                     /gene="rsmA"
                     /locus_tag="VW191_000156"
     CDS             complement(31424..32296)
                     /gene="rsmA"
                     /locus_tag="VW191_000156"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU9623006.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(32427..32735)
                     /locus_tag="VW191_000157"
     CDS             complement(32427..32735)
                     /locus_tag="VW191_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623007.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32728..33369)
                     /locus_tag="VW191_000158"
     CDS             complement(32728..33369)
                     /locus_tag="VW191_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623008.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(33371..35401)
                     /locus_tag="VW191_000159"
     CDS             complement(33371..35401)
                     /locus_tag="VW191_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9623009.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMTN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GARPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLTGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(35670..36569)
                     /locus_tag="VW191_000160"
     CDS             complement(35670..36569)
                     /locus_tag="VW191_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9623010.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQGLTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(36641..37201)
                     /gene="rnmV"
                     /locus_tag="VW191_000161"
     CDS             complement(36641..37201)
                     /gene="rnmV"
                     /locus_tag="VW191_000161"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEU9623011.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(37201..37974)
                     /locus_tag="VW191_000162"
     CDS             complement(37201..37974)
                     /locus_tag="VW191_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU9623012.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(38280..38945)
                     /locus_tag="VW191_000163"
     CDS             complement(38280..38945)
                     /locus_tag="VW191_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEU9623013.1"
                     /translation="MTILGKDTVQQSAKGESVTQEATPEYKLENTPGGDKGGNTGSSD
                     ANANEGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKNAIEKAAKNKQDEIKGAPL
                     SDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGVQAIAMVTVPKRPVAPNAA
                     PKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALASLGLVVATSGFALLGRKTR
                     RRK"
     gene            complement(39208..39342)
                     /gene="rpmH"
                     /locus_tag="VW191_000164"
     CDS             complement(39208..39342)
                     /gene="rpmH"
                     /locus_tag="VW191_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU9623014.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(39487..40701)
                     /locus_tag="VW191_000165"
     CDS             complement(39487..40701)
                     /locus_tag="VW191_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9623015.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(40857..41150)
                     /locus_tag="VW191_000166"
     CDS             complement(40857..41150)
                     /locus_tag="VW191_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623016.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            41312..41764
                     /locus_tag="VW191_000167"
     CDS             41312..41764
                     /locus_tag="VW191_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU9623017.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(41799..43187)
                     /locus_tag="VW191_000168"
     CDS             complement(41799..43187)
                     /locus_tag="VW191_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9623018.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            43253..44215
                     /locus_tag="VW191_000169"
     CDS             43253..44215
                     /locus_tag="VW191_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEU9623019.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(44212..44403)
                     /locus_tag="VW191_000170"
     CDS             complement(44212..44403)
                     /locus_tag="VW191_000170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623020.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDETDEVS
                     NHTYGKATLTWFAEIFEEY"
     gene            complement(44467..45477)
                     /gene="ccpA"
                     /locus_tag="VW191_000171"
     CDS             complement(44467..45477)
                     /gene="ccpA"
                     /locus_tag="VW191_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEU9623021.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(45902..46501)
                     /locus_tag="VW191_000172"
     CDS             complement(45902..46501)
                     /locus_tag="VW191_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623022.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDITILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(46503..47600)
                     /locus_tag="VW191_000173"
     CDS             complement(46503..47600)
                     /locus_tag="VW191_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9623023.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTVR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(47600..48337)
                     /locus_tag="VW191_000174"
     CDS             complement(47600..48337)
                     /locus_tag="VW191_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623024.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(48339..49223)
                     /locus_tag="VW191_000175"
     CDS             complement(48339..49223)
                     /locus_tag="VW191_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623025.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(49210..49404)
                     /locus_tag="VW191_000176"
     CDS             complement(49210..49404)
                     /locus_tag="VW191_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623026.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(49401..49607)
                     /locus_tag="VW191_000177"
     CDS             complement(49401..49607)
                     /locus_tag="VW191_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623027.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        14559 a        11203 c         9488 g        14605 t
ORIGIN      
        1 taatactaaa tccgaattaa ttccagaaag agtcagtcaa gatataaaat ttgataaaaa
       61 aatagaatta aaagatattt ggtataaata tggattattt gatgattatg ttttgaaagg
      121 aataaatgtt actattaaaa aaggagaaac tgttgctatt gttggagaat caggttcagg
      181 taagagtaca ttagctaaaa ttttattagg tttattagaa cctaatattg gttcaataga
      241 agttgatgga gtagaaaaag aagaaattgg tcaaacattg tatagaaaga tttttggagc
      301 agtgttacaa aattcaaccc taagttatgg taccttaaga gagaatttga catttggaca
      361 ctttgtttca gatgaagaat taatgacaaa tctaaattca attggtctta gcaatgtagt
      421 taaatcttta cctcttggat tagagacaat catcgctgaa gaaggtaata acttttctgg
      481 agggcagcag caaatgatac ttttagctcg ttgtcttttg tcgaaacctt cggtagttgt
      541 tttggacgaa gcaacaagta gtttagataa tttatctcaa caaattacaa cttcttactt
      601 aagtgaaatc ggtaccacta agattttaat tgcccatcga ctagatacta tcaagtctgc
      661 agataagatc ttagtaatgc ataatggtga aattgtagag attgggaccc atagagaact
      721 tcttgaacta ggaggcattt ataagcaatt gtattcaaat aattagtttt tgattaaaag
      781 ggtaaattta tgaagattat gaaaaaaaaa tattggactt tagcgatatt attcttttgt
      841 ttgttcaata attctgttac tgctcaagaa atacctaaaa atcttgatgg caatataact
      901 cacactcaga ctagcgaaag tttttctgaa tctgatgaaa aacaggttga ctattctaat
      961 aaaaatcaag aagaagtaga ccaaaataaa tttcgtattc aaatcgataa gacagaatta
     1021 tttgtaacaa cagataaaca tttagaaaaa aactgttgta aattggaact tgaaccacaa
     1081 ataaataacg atattgttaa ctctgaaagt aataatttac taggcgaaga taatttagat
     1141 aataaaatta aggaaaatgt ttctcatcta gataatagag gaggaaatat agagcatgac
     1201 aaagataact tagaatcgtc gattgtaaga aaatatgaat gggatataga taaagttact
     1261 ggtggaggcg aaagttataa attatattct aaaagtaatt ctaaagtttc aattgctatt
     1321 ttagattcag gagtcgattt acaaaatact ggattactga aaaatctttc aaatcactca
     1381 aaaaactatg tccccaataa aggatattta ggaaaagagg agggagagga aggaataata
     1441 tcagatattc aagatagatt aggtcatggt acggctgttg tagctcaaat tgtaggggat
     1501 gacaatatta atggagtaaa tcctcacgtt aatattaacg tctatagaat atttggtaag
     1561 tcgtcagcta gtccagattg gattgtaaaa gcaatttttg atgctgtaga tgatggcaat
     1621 gatattatca atcttagtac tggacaatat ttaatgattg atggagaata tgaggacgga
     1681 acaaatgatt ttgaaacatt tttgaagtat aaaaaggcta ttgattacgc gaatcaaaaa
     1741 ggagtaatta tagtagctgc attagggaat gactccctaa atgtatcaaa tcagtcagat
     1801 ttattgaaac ttattagttc acgcaaaaaa gtaagaaaac caggattagt agttgatgtt
     1861 ccaagttatt tctcatctac aatttcggtc ggaggcatag atcgcttagg taatttatca
     1921 gattttagca ataaagggga ttctgatgca atatatgcgc ctgcaggctc aacattatct
     1981 ctttcagaat taggacttaa taactttatt aatgcagaaa aatataaaga agattggatt
     2041 ttttcggcaa cactaggagg atatacgtat ctttatggaa actcatttgc tgctcctaaa
     2101 gtttctggtg cgattgcaat gattattgat aaatacaaat taaaagatca gccctataat
     2161 tatatgtttg taaaaaaaat tctggaagaa acattaccag taaaaaatgg tataaaagtg
     2221 ttaaatatac caaacgtatt gagatatgat ttgaatatgt tacaattaga atataaaaat
     2281 gaacaaagtt gggatagttt catagataat gttaatttaa ttgagttgga agagagaatt
     2341 caaactacta ttggaattaa acaaataaac acacacaata ttattactat tgcccgagaa
     2401 gggtactctc aaaattattt acctaacact tcagaaaata catataattc attacaagtc
     2461 agtttagttg gagtattact actttttata agtatggtaa atattttatg ggctaaaaaa
     2521 agtaaatgaa aataaaattt ggagccctct gaaaaagtaa gtcctacagt tcaactaaaa
     2581 tgagtcaaaa gatgaatcac cttgatgtag gggagtttgt cttattgctg cctgaacacc
     2641 tccgttcaga ggaagaacat tataaatctg tttttgaaga cgacttaacc agtcgcatat
     2701 ctagtcaaga tgaacgacag caaatgactg ctacggtagg ttatttagaa tcaggtcagg
     2761 atcgttttgt gtataatacg acccctattt cttaccagca gtttttgaaa gatccaatca
     2821 tcattgttat aacaccccaa tcaactggtc cacagtccat tttgttttgg atagacgcag
     2881 tacagaacta cgttctcttt aatcaattgt ctgatgccca ggagcttatc cagagacaag
     2941 gcattgaaaa ttgggtctca gaaatgcaaa caggttacca caactacatc acattattgg
     3001 ataatatcca gagggaacgt tgggtaatgc tagcaggagc tgtgcttggg attgcaactt
     3061 caatcttgtt gtttaacact atgaataggc tctactttga agaatttaga cgtgccattt
     3121 ttatcaaacg cattgcaggt ctcaggttct tagaaatcca tcgcacttat ctctttgctc
     3181 aactgggtgt gtttttactg ggatttgttg cgagtgtatt tcttcaggta gagataggag
     3241 ttgctttctt agtcttgtta ctctttactg gtctatctct tttacagtta catgtccaaa
     3301 tgcagaaaga aaacaagatg tccatgcttg ttttgaaggg aggttaatat gattgaactt
     3361 aaacaggtga gtaaatcttt tggagaacga gagttatttt cgaatctttc aatgacattt
     3421 gaggctggaa aagtctatgc cttaattggt tcaagtggta gcggaaaaac aaccttgatg
     3481 aacatgattg ggaaattaga accttatgat gggacgattt tttaccgagg taaagacttg
     3541 gccaattata aatcaagtga ttttttccgt cacgaattgg gctacctctt ccagaacttt
     3601 ggcttaattg aaaaccaaag tattgaagaa aaccttaagc taggtctcat tggtcaaaag
     3661 ttgagtcggt cggaacagcg gttgaggcag aagcaggctt tagaacaggt cggcctggtt
     3721 tatcttgacc tagataagcg catctttgag ttatcgggcg gagaatcgca acgggttgcc
     3781 ttggcaaaaa ttatcttaaa gaatccaccc tttattctgg cagatgagcc aacagcttca
     3841 atagacccag caacctctca gttgattatg gagattttgc tatctcttcg agatgataat
     3901 aggctaatca ttatcgcaac acataatccg gcaatttggg agatggctga tgaagtgttc
     3961 acgatggatc atctgaaata aaaatccttg tttttaattg cacgatgagt tactgaaata
     4021 ttatcatgaa tcaagaattg gagttaattt agaattgtac ttaatttaga attgtacttt
     4081 attaatattg aggtaacttt ttcttgataa aggaagaaat aatggagagg aagttagaat
     4141 gaaaaaattc gacaattata ttattgagaa gccttgcgat tctaattcag ataaactgca
     4201 aaaaatctta ataattgaaa gtttggtaga tgatattttg caattttctc tcagaatcaa
     4261 taatagtgta ggagagattt tcctcctaca accgttttaa aagaaaacta tctttattcc
     4321 atgttatttt gaggaagata ttgtgaaagt caaagatgat gataaagttg agtggaattt
     4381 gttagaattt caaaaattta gagcattttt ggcttagtaa tctgtgttga aggctcaaaa
     4441 cctatggtaa aaaagtagct ttgaaaacgt attgcctcca aagatttagt taaataatga
     4501 tttaacacaa aaagaaatta ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag
     4561 gtgggaaaaa cttgcgattt tcacagagaa aggaagaaaa agtatagaaa tatagtcaat
     4621 tgaaacaaga acaggataaa agaacctttt gtgccatatt tttctccttt cgctttacaa
     4681 ttggattgaa cacctttatt gtatcgcgtt tggagttttt ttggtataac cttcgacgca
     4741 cacccgcata gcgggtgttt tttttgtctc gcacctaacg gagcgagaca aactaatagt
     4801 cacttaatca aaaaacgcac catatcaaaa actaaaaagt ttgatatcat gcgtcatgtc
     4861 ttaaactaat tgactatact ttctattcaa atgagctttt aaccaattga ttgagccaat
     4921 ccactcttaa aaccaaagag caatttctcg cttagctgac tcttctgaat ctgaaccatg
     4981 tacaacattt tggataatct cattttctcc agcagctttt gcaaaatcac ctcgaatagt
     5041 gcctggtaaa gcttcttctg gacgagttgc acccatcatg gtccgccaag tttcgattac
     5101 tttgggacca gaaatgacac ccacaagaac tggacctgaa gtcatgaatt cacgaatcgg
     5161 tgggtaaaaa ctctgaccaa ccaagtcctg atagtgctgg tcaatcaact cttctgaaac
     5221 ctgtgaacga aactccaatt tttcgattgt aaatccacgt tgttcgatgc gctttaacac
     5281 ttcacccact agccctcttt ttacaccatc tggtttgatg ataaagaatg tttgttccat
     5341 acccgtctcc tttgtcagct tctttctttt attttaccac atttcgtgga aaaatggaga
     5401 aagttttcag aagagagaat gagagaaccc tcgggttctc tcattctctc ttattctact
     5461 gtttcttcca cagtttcaac ggcagtatcc acaactactt ctgttgtttc ttcatttcct
     5521 tcttcctcta ctggaggatt aaggtattct tcttcgttga cagcatgtgg ttcaaggtta
     5581 cggtaacggg ccataccagt accagctggg atgatcttac cgatgataac attttcttta
     5641 agtccaagga gatggtcttt cttaccacgg atagctgcgt cagtaaggac acgagttgtt
     5701 tcctggaagg aagccgctga caagaaactg tttgtttcaa gtgaggcttt ggtaattccc
     5761 ataaggactg ggcgacctgt cgctggaact ccacctgcga taaggacatc tttgttggca
     5821 tctgtaaagt cattgatatc catgagggta cccatgagaa gatctgtatc acctggatcc
     5881 atgacacgga ctttacggat catttgacga accattacct cgatgtgttt gtcaccgatt
     5941 tctacccctt ggctacggta aactttttgt acttcaccga gaaggtacgt ttcaactgac
     6001 aagacatcac gaactgcaag gagacgtttt ggttggatag aaccttctgt cagagcagca
     6061 ccacgcgcta cttggccccc aacttcgaca cgcatacgag ctgtaaatgg aacgacatat
     6121 tcaccttcgc cagtttcacc cttaacaaag actttcttgg tacgagttga tgcatcttct
     6181 tcgatagcag taacttgtcc tttaacctct gtaataaccg cttccccttt aggattgcgg
     6241 gcttcaaaga tttcttggac acgaggaaga ccctgagtga tatcggtatt tgaggcaacc
     6301 ccacctgtgt ggaaggtacg cattgtaagc tgtgtaccag gttccccgat agattgggca
     6361 gcgattgtac caactgcttc accaacttca accgcatcac cagtcgccaa gttgataccg
     6421 taacagtgac ggcagacacc gtgacgagtg ttacatgtaa atacagaacg gatagtcact
     6481 tcttccacac cagcattgac aatttcacgc gccttgtctt ctgtaatcaa ttcatttgga
     6541 ccaataatca ctgcaccagt ttctggatgt ttaacagttt tcttagtgta acgaccgttg
     6601 agacgctctt cgagagactc gatcatctct tttccttctg cgatagaacg gatcaagaga
     6661 ccacggtcag ttccacagtc gtcctcacgg atgataacgt cttgggcaac gtcgaccaaa
     6721 cgacgagtca agtaacctga gtcggctgtc ttaagggccg tatcggtcat acctttacga
     6781 gcaccgtgag ttgagaagaa catttccaat accgacaaac cttcgcggaa gtttgaaagg
     6841 attggcaatt ccatgatacg tccattcgga gcagccatca gaccacgcat accggcaagc
     6901 tgtgagaagt ttgagatgtt accacgggct ccagagtcca tcatcataac gattgggttc
     6961 ttaggatctt gattagcaat caagcgtttc tcaagttttt cacgggcagc acgccattca
     7021 gctgtaacag cattgtaacg ctcgtcgtct gtgatcatac cacgacggaa ttgtttggtg
     7081 atttgttcga cacgtttgtg tgattcttca atgatttcag ccttgtcatc aacgactggg
     7141 atatcggcaa tacccactgt caatcctgca agagttgagt ggtggtaacc gaggttcttc
     7201 atgcggtcaa gtagggcaga agtttctgtc gtacggaaac gtttgaagat ttcagcgatg
     7261 atatttccga ggtttttctt cttgaatgga gggttgagct caagattgct gatagcttcc
     7321 ttgatagctc caccaagtgg caagaagtat ttagctggaa caccttctgt caagttggca
     7381 ttgtttggtt cttgcaagta tggtagcccc tctggcatga tatcgttgaa gagaatttta
     7441 ccaactgttg taagcaagac cttatgtctt tgctcttctg tccaaggctt gttgaggctg
     7501 tctgttgcga taccaacacg tgagtggagg tgaacataac cattgcggta agccataacc
     7561 gcttcgtcac ggtctttgaa gaccattcct tcaccttcgc gaccagcttc ttccatggtc
     7621 aagtagtagt tacccaaaac catgtcctga gatggagtaa ctaccggttt cccatctttc
     7681 gggttcaaga tgtgctcagc agctagcatg aggatacgag cttctgcttg tgcttcttct
     7741 gaaagtggta cgtggatggc catttggtcc ccgtcaaagt cagcattgta ggcttcacag
     7801 acaagtgggt gcaagcgaag agccttacca tcaatcaaga ctggctcgaa ggcttggata
     7861 cccaaacggt gaagggtcgg tgcgcggttc aaaagcactg ggtgttcttt aatcacttct
     7921 tcaaggatat cccagatacg ctcatctccg cgttccacca agcgtttagc tgctttgacg
     7981 ttttgcacga tatcacgggc aacgatttca cgcatgacaa atggtttaaa gagttcaatc
     8041 gccatttcac gtggcacacc acattggtac atcttaagag ttggaccaac ggcgataacg
     8101 gaacgtcctg agaagtcaac acgtttaccg agcaagtttt gacggaagcg tccttgttta
     8161 cctttaagca tgtggctcaa tgatttcaat ggacggctac ctggtcctgt gattggacga
     8221 ccacgacgac cattgtcaat caaagcgtca actgcttctt gaagcatacg cttctcattt
     8281 tgaacgatga tacctggtgc atttaactca agcaaacgag ccaaacggtt gttacggttg
     8341 ataacacggc ggtaaaggtc attcaagtca gatgaggcaa aacggccacc atccaactgc
     8401 aacattggac gaagatctgg tgggataacc ggaaggatgt taagaatcat ccattcaggt
     8461 ttgtttccag acttgtaaaa ggcatccaaa acatccaaac gacggatggc tttgacacgt
     8521 ttttgtccag tagcagtttt caattcttct ttgagttcag caatttcttt ttcaagatct
     8581 acttgcttca aaaggtcttg gatggcttcc gcacccatct tagcaacaaa tgaaccataa
     8641 ccatattcgc gcaagcgctc tcggtattcg cgctctgtca tgatagactt gtgctcaagt
     8701 ggtgtatcct taggatcaat caccacataa gccgcaaagt agataacttc ctcgagggca
     8761 cgagggctca tatcaagggt caagcccata cggcttggaa tccccttgaa gtaccagatg
     8821 tgagatacag gagctttcaa ttcgatatgt cccatacgct cacgacgaac tttcgtacgc
     8881 gttacttcaa ccccacagcg gtcacaaaca attcctctgt aacgaatgcg tttgtactta
     8941 ccacaagcac attcccagtc ttttgtagga ccaaagatca cttcatcaaa gagtccttca
     9001 cgttctggtt tcaaggtacg ataattgatt gtttcaggtt ttttgacttc tccataagac
     9061 catgaacgga ctttacttgg agaagctagg gtgatttgca tacttttaaa acgatttaca
     9121 tcaaccacta tttcttccct ttctattcta agtgaactgc ttattcttgt tcagcagctt
     9181 cttctgttgc ttccgctttt gttgctttct cagcttcttc agcttcaaag gctgctttag
     9241 cctcttgggc tgctttttcg cgggcttttt caaggtcatc tacgtggatg acatcttcgt
     9301 ccattccttc atccaagtcg cgaagttcca cttcttggtc atcttcgtct aggacacgca
     9361 tgtcaagacc aagagattgc aattctttga caagaactcg gaaggattct ggaacacctg
     9421 gttttggaat tggtttgcct tttgtaatag cttcataggc tttcaaacgt ccgttgatat
     9481 cgtccgactt gtaagtcaag atttcttgaa ggacatttga cgcaccgtag gcttcaagag
     9541 cccaaacctc catctcaccg aaacgttgtc caccaaactg agctttacct ccgagtggtt
     9601 gttgggtaac agttgagtaa ggtccgactg aacgcgcgtg caatttatcg tcaaccatgt
     9661 ggtggagttt gatcatgtac atgactccaa cagaaacacg gttatcaaat ggttcacctg
     9721 tacgtccatc gtaaaggatt gtcttggcat cgctatccat acctgcttct ttaacagttg
     9781 accaaagatc ttcagaactt gctccatcaa agactggtgt cgcaatgtga ataccaagag
     9841 tacgagctgc cataccaagg tgaagctcca taacctgacc gatattcata cgtgatggca
     9901 ccccaagtgg gttcaacatg atgtcgactg gagttccgtc tggaaggtaa ggcatgtctt
     9961 ctacaggaac gatacgagag acaacccctt tgtttccgtg acgtccggcc attttatctc
    10021 cgaccttaat cttacgtttt tgagcgatgt aaacacgaac caacatgtta acacctgatt
    10081 gcaactcatc tccatttaca cgtgtaaaga tcttaacatc acgaacgaca ccatcggcac
    10141 cgtgtggtac acgaagagaa gtatcacgca cttcacgaga cttgtctcca aagatagcgt
    10201 gcaagagacg ttcttcagct gaaagatctt tctcaccctt aggtgttact ttacctacaa
    10261 gaatatcacc ttctttaacc tcagcaccaa tacggataat ccccatttcg tcaaggtctt
    10321 tgagggcatc ttcaccaacg tttggaattt cgcgagtgat ttcttcaggc ccaagctttg
    10381 tatcgcgcgt ttctgattcg tattcttcaa ggtgaacaga tgtgtagaca tcgtccttca
    10441 ccaagcgttc gctcatgata acggcatcct cgaagttgta accttcccaa gtcatgtagg
    10501 caacgattgg gttttgtcca agcgccattt ctccattttc catagaaggt ccgtcagcga
    10561 tgaaatcgcc tttttcaacg acatcaccaa cttttacgag agtgcgttgg ttgtaagcag
    10621 tacctgagtt tgaacgacgg aatttttgga tgtggtaaac atccaatgaa ccatcttcac
    10681 gacgaacttc taccttgtca gcatctgcgt aagtaacttt accatcatac tgagcaatca
    10741 cagccgcacc agaatcgtgg gctgcttggt attccatacc agtaccaacg taaggtgcct
    10801 gaggattaat caatggcaca gcctgacgtt gcatattggc tcccatgagg gcacggttgg
    10861 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactacc tgttttggtg
    10921 aaacgtccat gtagtcaaca atattagctg gatactcttg gttgacccct tggtgacgtc
    10981 ccatgacaat cttctcagca aaggttccat cttcattcag acgagagtta gcctgagcta
    11041 cagtatattc atcttcttca tcagctgtca accaaacaat ttcgttcgtg acaacacctg
    11101 tttcacggtc aaccttacgg tatggtgttt gaacaaaacc atatttgttc aagtgtccgt
    11161 aagatgacaa gttattgatc aaaccgatgt taggtccttc aggtgtttcg attggacaca
    11221 tacgaccata gtgagtgtag tgcacgtcac gcacttcata tccggcacgg tcacgagtca
    11281 aaccaccagg tcctaaggct gacaaacggc gtttgtgaga caactcagaa agcgggttgt
    11341 gttggtccat gaactgtgac aactgtgatg aaccaaagaa ttctttaact gcagctgtta
    11401 caggacggat attgataatt tgttgtggtg tcaagacttc attgtcctga acagacatac
    11461 gttcacggac attacgttcc atacgagaaa gtcccaaacg tacttggttg gcaagcaatt
    11521 caccaaccgc acggatacga cgatttccaa ggtggtcgat atcatctaca cggccaagtc
    11581 cttcagccaa gttgaggaag tagctcatct cagcaaggat atctgcagga gtcaccgtac
    11641 gaaccttgtc atctgggtta gcattaccaa tgatcgttac gacgcgatct ggatcagttg
    11701 gagcaacaac cttgaatttt tgaagaacaa caggctcagt cacaacggct gcatcgtttg
    11761 ggatgtagac aatcttgttc aagtcgccat ccaaatggct ttcaatgctt tcaatcacgc
    11821 tacgagtcat aatcgtacca gcttctacca agatttctcc agtttcaggg tctaccaatg
    11881 gctctgcaat ggtttggttg agcaaacgtg ttttaacatt gagtttttta ttgattttgt
    11941 aacgaccaac tgctgccaag tcatagcgac gtgggtcaaa gaagcgagcc acaagcaagc
    12001 tacgtgagct ttcagctgtc ttaggctcac ctggacgaag gcgttcgtaa atttctttca
    12061 aggcttcgtc tgtacgagag tccattggat tcttgtggat atctttttca acagtgttgc
    12121 gaaccaattc gctgtcacca aagatatcaa agatttcatc atcacctgag aaaccaagag
    12181 cacgaaccaa ggttgtaaat ggaatcttac gagtacggtc gatacgagtg taggtgatat
    12241 cttttgagtc gctttcaagt tccaaccaag ctccacggtt agggataaca gttgaaccat
    12301 agcccacctt accatttttg tctactttgt cgttaaagta aacacctggt gagcggacca
    12361 actgagaaac gataatacgt tcaccaccat tgatgatgaa agtacccatt tctgtcatga
    12421 ttgggaaatc accaaagaaa acttcttggg tcttgatttc gcctgtttct ttattgatca
    12481 aacggaaggt tacaaaaatt ggtgctgagt agctagcatc gtggatacga gcttcttcta
    12541 gcgtgtattt tggttccttg atttcatatc caacaaattc caactccatt gtgtctgtga
    12601 agtttgaaat tggcaataca tcttcaaaca cttccttaag accgtggtct aggaaagctt
    12661 tgaatgagtc agtttgaatt tcaatcaaat ttggtaagtc aagaacttct ttgattcttg
    12721 aaaaactacg acgggtacga tgtttcccgt attgaacgtc atgtcctgcc aagatgattc
    12781 tcctttgtaa ataagttcca agccttgtca atcaggcttt tctaatcgtc atatggttgt
    12841 aaacccctta tcaccgtgtc ctcttgacga attttcagaa tctttaagcc tctgttacaa
    12901 atgctcataa tcttgaaaaa aagcacaaaa agagcagcta aatctgactt tttcagaaga
    12961 tttaactgct gtgagccttg tctggacaat atttcagaca aaacctacga caaatgatta
    13021 cccatattat accctattta gctagatttt tcaaggggtt tcagtaggtt tttggtaaat
    13081 tttttcccat agaaaacttg gcatcacatt cgaatcacgc tatggtacaa aaaactgaaa
    13141 aaactattga ctgaaaatca ttttcaaggt ataataataa acgttaaggc ggtatagcca
    13201 agtggtaagg cacggctctg caaaagcttg atcgtcggtt caaatccgtc taccgccttc
    13261 tataacttga tttatcaggt ttcaaatgaa cagaaagccc aatttgaagg gcttttctta
    13321 ttttccctcg aataaatacg tataacttta aaaacttttg gagcgagttt gtggcagagt
    13381 tctttccatg gcataattcc cttttgaaat caattgaaaa aagcttcgtc acgcctggtg
    13441 acaaaagcct ctagtttatt ctgtttcttc ttctatttcg acttctgtga tttggacttt
    13501 taccttggta acacgtccat tcttcacttt atcattggtt aagataaact gtttgttttg
    13561 gctgaccaat tcatagctga gtttctcagt cgttggaatg gttcccactc ctgtcaaata
    13621 ataaccagcg atagtatcaa cgtcatcact ttccagttca acattaaagt aattattgaa
    13681 atcattgagg gtcatagttc cttgaacaat gtaggtatcc tcgccaatct gatgaacatc
    13741 tattgctgct ttgtccgttt catcatcaat ttcaccaaca atttcctcta agaggtcttc
    13801 caaagtcacc aaaccagcca taccaccgta ttcatccagc aagattgcca tttgtctttg
    13861 ggtatttcgc agttctttta gcaagtcatc cacaaaaata gtttcaggta caaaaagtgg
    13921 atcttgtaaa attctcttcc aaacaatatt gtcaaaaccg tccacaaagc ctgccttaag
    13981 gagactcttg gtgtgaatga ttccaattac attgtcctta tccccatcat aaaccgggat
    14041 acgagaataa ttttgtttta aaatactttg gataatggct tgactatcat cctgaatatc
    14101 caccataaag gcatccgttc gaggaaccat aacctctcgt gccatcagtt catcgagcga
    14161 aaagacacct tgtagcatct caatctcatc agcatccaat gtttcttcac tatttgtcag
    14221 catataggca atttcatcac gggtcatctt ttcatccgca tcatcgaatg acataggagt
    14281 caaatggctc aagaaattgg tcgaagcagc taaaagccaa acaaaaggac tgactagttt
    14341 tccgatccca atgataaccg gcgctgtacg aattgccaag gcatccttta gattaagagc
    14401 gattctctta ggatataatt ccccaaaaac gatggaaata taggtcaaaa atgccaagga
    14461 tagaaaagtt gccacggctt gtgctgtttc gccattccca agccaagagg caatcacacg
    14521 tcctagagta tcagttaaac tcgcccctga taagattgta atcagggtga ttcctacctg
    14581 aatggttgat aaaaagtggt taggattttc tagtaccttc agcaggcgga tgtagcgtct
    14641 gtctccttct tccgcctttt gttcaactcg ggcacgatta agagaaacca tggccatttc
    14701 tgtggctgag aaaaaggcat ttaataccgt caagataaac aataaaacaa attgtagcaa
    14761 caaattctga ctacttgggt cttccatagt tcttctccta aaatagtatc ttatccctta
    14821 ttatatcaca aaatttaatc caatacatat tattttttct taccgtaact tctcaaagta
    14881 acttccatct ctctcccaaa actggaagtt agttttagaa gttgctattt tttcttggta
    14941 cctgttctag ccctgcttaa actagtaaaa agaactccta catccataag tgacatagga
    15001 gtttaggctt atcttttact gagttacagt tacttctcca gttcggtaat ccagttgaag
    15061 tcccttttga acattgggaa ctagaacatt gaattccaat tctccatccg aagacttggc
    15121 ttcaatctgt gaagctgagg gaactaaatc ctcgtttgaa gcgtagtaaa gggttacacg
    15181 gtaacggaca cgcttgtttt ggtccaaggc tttacgcacc ttgctttcat agtagttttg
    15241 accagtcgaa tactcggctt gtgcctgatt tgcccaggct gtctgaacag caatgttttt
    15301 aggattgctt gttgaggcat caaaaccatc caaaccaccg attaaggcat agcctaacaa
    15361 atgacctcta tcgactgcat gggtataaga gccctttaga ttcttgacct gatgccaacc
    15421 tggaggagtc caagaagttg aaccattccc agtttcttta cgattcttgt actgacgagt
    15481 ggccttagac aagagggcat tagctacggt tggaacagtt tccttgccca ctgtctttgt
    15541 tttattgtca gcgtagggct tacttgaaac cttggcatct agatttgttt tattaccatt
    15601 gacgataaaa gcacctgagc cattccactc cagactcccc tttatttgac tcttgactgc
    15661 gtctgttaag acactctctg ccaatgcttg actaggagct tcagacgctt gttttttctg
    15721 actaagattg gttttgggac tattaggtgc cgacggcatc tgcttgatat aatagctccc
    15781 tgtagacaaa agcaataaca ctagcagtcc gattagtgtc tgtcttgttt ttttgttcat
    15841 atttctcctt atctttagaa aaaggctggt tctccagcct aatttcctgt aaatttatga
    15901 atcaattcct gccattttgc tggagacagg atagcccatg gatcttgacc cttgcccaag
    15961 attccataac ctaccattaa accgattcct agagccagag tacctaaaat cagtactatg
    16021 atgactaaaa gtaaacgctt gactacgtag cttgatttct tattcatctt catcacttgc
    16081 ctcaatccgc tgaatcttag cacctagctg cgccaacttc tcatggaaac cgtagtaacc
    16141 tctatccaag tgaaccaatt taccgaccac agtttctccc tgtgctacca aacctgtcaa
    16201 aatcaaggct gcactggcac gaaggtcagt tgaaagaact tctgctccct gcaaaggctg
    16261 tccaccaaca atacgagctg tatcacggat aatctcagaa tgcaagccca tgcggcgcat
    16321 ctcttctagg tgttggaaac gattttcgaa aactgtctcc accatggttg attcgccttt
    16381 tgcaactgtc atcaaggctg taaattgagc ctgcatatct gttggaaatc ctgggtgggg
    16441 caaggttttc acatgaacag cttttagatt ttctagttga gaacgaacac gaattccttc
    16501 gtcttcttca attacttcaa cacccatttc aagtaacttg gcaatcaagg gacggttgtg
    16561 ctcccagaca gcgtctcgaa tcaagacatc accaccagtc atggcagcag ctaccataaa
    16621 ggttcctgct tcgatacggt cttggactac attgtgagtc gtaccatgaa gtttctcaac
    16681 accagtaatg gttatagtct ctgtaccagc acctttgacc ttggctccca tttcattaag
    16741 gagaatggct aagtcaacaa tctcaggctc acgcgcagca ttctcaatca ctgtcacccc
    16801 atcagccaga gtcgctgcca tcatcaagtt ctgcgttgca ccaacacttg gaaagtccat
    16861 atagatatga gcgccatgca agcgttctgc cttggcttcg atgtaaccag ctgtctgact
    16921 aatcttaacc cccatagctt ccagaccttt caaatgaaga tcaataggac ggctaccaat
    16981 cgtacaacca cctggcatgg ataccttggc atgacccaca cgggcaagga ttggccctaa
    17041 tacaacgatg gaggcgcgca tcttgctgac atacttgtaa ggggcttcct cagtgatgtc
    17101 gccagtagca tccaccttga caagatgagc ttcctcatca aagtcaacct tggcattcaa
    17161 accaccaact acctgattca taataaagac atccgacaaa atcggaacat tctgcaagac
    17221 ggtctttcct tcacttgcta gaatagtcgc tgccaacaag ggtaagactg cattttttgc
    17281 tccctcgatc gtcacgcttc ctaccagacg attatcgcca ccttgaacca caattttatc
    17341 catactcgtt tcctttactc ttgatttcat aataatcctc taagcgcttc ttgccacaaa
    17401 tgatacaggc taataaagaa agaactcagg atgtagccta tgacaatgct aaaaaaggct
    17461 actaataaac gaacttttct tgtattatca tctgtcactt ttaaaacctt ctcccatcta
    17521 acaagatcct ttaaaaggta aaaactcaca taaataaaga acatatgact acttagagtg
    17581 aataataatt gaaccatttg actattatac catcttctct gtttgtgcgc tagtttggaa
    17641 acttcactca aaaattaggg attgtttttc aagtaatcaa tcgcatcttg tagggtttta
    17701 acgggcacga ttttcatatc cgtcttgatt gttttagccg cttctagggc tgtttggtag
    17761 ttgtttttcg cgtccggatg cgccttttgt tcttcttcgc taacagggtt atcaggagca
    17821 aagaaaatag cagcacctgc cctagccgaa gctacaacct tcttatcaat gcctccaatg
    17881 tctcccacat taccatcgcg gtcaatggta cctgtaccgg caacaatacg accattacga
    17941 agatctgggt gagctatttg agtatagata gctagactaa acatgagacc agcacttggg
    18001 ccgccaatac cagctgttga aaagctaatt gggacattgc tgattacctc tgtacgatca
    18061 atcaagccga ttccaattcc atttttgcca ttttccaagg tgataatctt tccttctgca
    18121 gacttggttt gcccatcctc ttcataggtg accttgacgg aatcccctaa tttttgagaa
    18181 ctgacgtaat caatcaagtc tttggaacta tcaaaggtct gatcattgac tgctgtgact
    18241 gtatcagaga tattgagaat ccctttaaag gttgaattat ccgtcacatt caaaacataa
    18301 actccaaagt acttgagttc gatatcctta ccagctgttt ttagtccttg atacttggcc
    18361 atattttgcg atgtttgcat gtagaattga ttgattcgca taaattcaac atcggaagaa
    18421 ccacctgtag tctcctgagc actacgaata tctgtaaaag gtgtcaacca agcataaatc
    18481 atatgagcta aagtggcatg ttgaacacca accgtaacga attgataggc accagcttcc
    18541 ttatcttctg tgtcatttac tttaaggact tggcgaatat cttccgaacc acctggaacc
    18601 tctatataat aaggcaaggg cactacaaat gccaagaaag tcacaatcaa ggccgcaatg
    18661 acgtataagg gccatctaat cttttttttc atttcttatt tcctccaaaa tactctcggg
    18721 aacatggcag gcaatatcct gaccaaactt caaaagctct cgaacacctg atgaactgat
    18781 atagagatgt tcaggccggc tatgtaaata aatagtctct atatcagaag acagctgatg
    18841 attgtagtaa tcaaaactag cttcatattg caaatccgcc gcattcctca acccccgtac
    18901 taggaaagta gcccccagtc tttttgcaac atcgaccacc aattcatcat gagaagccac
    18961 gacttcaaca ttttccaaat gtcccaaagc cttttctagc ccccgtttac gattttcaag
    19021 agggagaaat ccttgtttgt ggggattaaa aaaaataccc acataaagct tatcaaaaag
    19081 tctgctcgcc cgttcaatga tatccagatg cccatttgtc atcggatcaa atgagcctgt
    19141 gaataagcca atcttatctg acatagactg tcaccttact aattccataa atcttttcct
    19201 tccagatacc cagacaggca atctcttctg gaagttcaac ggccttatcc gtctcgcaca
    19261 caaccataac atcttcagaa aaaagctctc tcttagccat tttttcaata tctgctacga
    19321 tttgttcctt ggcataggga gggtctaaga aaacgaggtc aaattcccca gatacctgtt
    19381 ccaatgccct ctctgcatcc atcttgagga gttgaaattt tccaacttcc ttggtcatct
    19441 ggatattttc agccacgatg gtctgagcct tacggtctcg ctccaccaaa acagcactgg
    19501 acatgccacg cgatactgct tcgatagata aaccaccact acctgcataa aggtccaaga
    19561 ctcgtccccc ttcaaagtag ggaccaatca tgttaaaaat ggctccccta accttatccg
    19621 aagtaggtct tgttgtcttg ccttctagtg tcttgagggg acgtccccca tagattcctg
    19681 atacgatttt catactgttt attataccaa attatagaca aaaagagaaa gaaaaccgaa
    19741 ccttgcggtt cgattctcta caaaatattt tcgtaagtat cgcgtacttc ttgaggccaa
    19801 acacttgttt gcacttctcc gatgtgtctc ttgcgaagta ggaacatggc catacgagat
    19861 tgtccaattc ctccaccgat tgtcaatggg aataggccat tcaacaaaga cttgtgccat
    19921 tccaattcca agcggtcttc atcacctgta atttccacct gacgtctaag agtttcttca
    19981 tctacacgaa ttcccataga agacaactca aaggctccac ctaaagactc attccagaca
    20041 agaatatcac catttagacc cttgtagcca ttctcagact cgcttgtcca gtcatcatag
    20101 tctggtgcac gtccatcgtg cggtttacca tctggcaact cgccaccgat accaatcaaa
    20161 aagacggctc caaattcttt acaaatcgca ttttcacgtt ctttcgatgt caagtctgga
    20221 tagcgttcta ccaattcttc tgtatggata aaggtgattt gttttggcaa gatagactcg
    20281 atgtcatagc gagcttcaac agctagctca gtcaggcgaa tagccttata aatcttctca
    20341 actgtttctt ttagataaac gatgttacgc ttaccatttg ggataacctt ctcccagtcc
    20401 cactggtcaa cataaacaga gtgggttgca tccaaggaat cctcatctgg acgaagggct
    20461 ttcatgtgga caaagagacc ctctccttca ccaaagccaa aacgagccaa ggtgtggcgt
    20521 ttccatttag caagtgagtg caccacttca taagtagcat cagggatttg gagaaccttg
    20581 accgatactg gattttccac accagacagg ttgtcctgca ttccgtcacc gaccttactc
    20641 aagataggac cttgaacttc gacaacttct agcttatctt tcaaatactg ggtaaaagtg
    20701 tttttgacaa aggaaatttc ttcttgttga tggataaaac ttttcttcat agacaactcc
    20761 tcaaaaattt aataaacgta ttatacacct atttccaaga aaagaaaaga gaaaatttaa
    20821 aaaaaatcag tgtcactcaa aacactgatt tcatagacct tttagtcatt gcgaccaaag
    20881 atacgtagaa tacttaggaa aagattgata aaatcaagat agatgctgag agccattgac
    20941 accacccagc ctgtcgctac acgaccttgt gattgttcat aggcaaggcg aatcttttgg
    21001 ttatcccaag caatcaaccc tgaaaaaacc aaaaccatgg ctacactaat catataatca
    21061 aagaagccac tagccaagaa aatattgact accatagcaa tcagtaaacc aatgagagct
    21121 gccatcattg ctcgaccaat cccactcaag tctttcttgg taaagatacc aactgccgcc
    21181 atgacaaaga agagaagggc gctcgataca aaggcagata aaactgtacc cggagtatag
    21241 aaagccacca caaaactaag ggtaaagccg tttaaaacgg agtaaagtaa aaatactgga
    21301 agagcagctg gactatttct tgaagccatg ctactggcaa cgaaaaccaa agccaactcc
    21361 gcaaaagtag caatggtcaa ccagagacgc ccctgcatca acaagtaaac caactgagac
    21421 tgaaagaccg tcaacataag gcctgatacc aaagcagata gtccgattcc cagaccaaca
    21481 aaggcataaa ccttagcgta aaattgattg agacctgcac ggtcatgtat aatagtgtga
    21541 ttcatccttt tctccttttc tgtgaacatc tttccttgat tataacaaaa aaagttaaaa
    21601 ttagcttaaa tggaagatta aaataccggc tgcaactgca acattcaaac tctcggcctg
    21661 ccccttcatg ctaatatgga ccaactggtc tgcactttca gccataaggg gactaattcc
    21721 ttgaccctca tttcccataa ctagtacaaa attttctata gaaggcagtt ctctgtaatc
    21781 aacagaatct ttagataggg ttgttgctag cactgggata gctacctttt tagtttcgtc
    21841 aagaagcgct ggactagtca tccggtaaat gggcagatgg aaatgactac cttgcatgga
    21901 acgtagggtt ttgagactgt agatgtctgc cgacttatct gaaacaatca ctcctgtaaa
    21961 acctgctgca tccgcagttc gaatgatagt tcctacatta ccgggatctt gcacatcttc
    22021 caaaaacaag aacttgccct gactcaaatc agcttgtcct acttcttctt tttgaaccac
    22081 cgccacaatt ccctgtggag tctgagaatc cgccaaatct agcaaaatat cctctgaaat
    22141 ccagacagtt tgcggaaaag cagctaactg atctcgataa ttttctaggg caaagatttt
    22201 ctcaatcgtc actccagctt ggacagcttc ttcaaacaag tgccagcctt caatcaaata
    22261 ggcagacttg cggtattttt tttggtgtaa tttcttggca ttttttacca cagaattggc
    22321 ttttgaggtt ataatagtca tagaaatatt ataacacaat caaaggggtt tggtatgcaa
    22381 aaggttagaa tgattgccca aggtagggta cagggagtcg gctttcgttg gggtgtttac
    22441 agcttggcac ttgaaattgg tggcatcaca ggtcgagtat ggaataacga cgatggcaca
    22501 gtggaaatct tagcccaagc agactcatct gctatcatgg caaaatttat ccaagaaatc
    22561 cgaaaaggac cgacaccttt ttcaaaagta agctacttag atgtcaaact aagcaacttt
    22621 cctccctact ctgactttaa aatcgcaaat taggtctcca gaactattgt atattttgta
    22681 aaaaaacagt agaatagaaa ggtataattt ttaaagaagg aataaaaaca gtgaaatcta
    22741 ttaaacgttt tgcactctca gctatgggag cggctatgtt gcttgtcttg actggttgtg
    22801 tcaatgtcga taaaaccaca ggtcagccaa caggatttat ttggaatacc atcggagccc
    22861 ctatggctga agctatcaag tacttcgcta ctgataaagg tctaggcttt ggtgtcgcta
    22921 tcatcatcgt aaccattatc gtgcgcttga ttatcttgcc acttggtatc taccaatcat
    22981 ggaaggcaac gcttcactct gaaaagatga acgccctcaa gcacgtcctt gagccacacc
    23041 aaacgcgtct caaagaagcg actactcaag aagaaaaact cgaagcccaa caagctctct
    23101 ttgctgctca aaaagagcac ggtatcagca tgtttggcgg tgtaggatgt ttccctatcc
    23161 tccttcaaat gcctttcttc tctgctatct actttgctgc ccaacatact gaaggggttg
    23221 ctcaagcaag ctacctaggc attcctctag gttctccaag tatgattttg gttgcctgtg
    23281 ctggtgtcct ttactatctt caatcgctcc tttcacttca cggagtagaa gatgaaatgc
    23341 aaagagaaca aatcaagaaa atgatttaca tgagcccact catgatcgtc gtcttctccc
    23401 tcttctcacc agctagtgtc acactttact gggttgtcgg tggtttcatg atgattctcc
    23461 agcaatttat cgtcaactat atcgttcgtc caaaacttcg caaaaaagtc catgaagaac
    23521 tggcaaagaa cccatcaaaa gcaagtgctt tctcaacacc aagtggacga aaagacgtta
    23581 cccctgaaca accaactgct attacaagca agaaaaaaca caaaaatcgc aacgctggaa
    23641 aacaacgttc gagataagaa gaaaaaggct tagctgattt gctaagtctt tttgcaagtc
    23701 aaaagcccga tgtttccatc aggcttcttt tctatccatg tacacgtttc atataatctt
    23761 gataactttc ggtatccatg agttttttag cgttcttgac gcgatctgct gttggtggtt
    23821 tgactccttc gagggaatat ggaattccaa gttcacgcca cttgaactca cccatggtgt
    23881 gataaggtag aatttcaaac ttatcaacat ttttgagggt cttgacgaac ttaccaagtt
    23941 caatcaagtc atcatctctg tctgtcaatc ctggaactag cacgtggcga atccagacag
    24001 gttttccaat atctgataga tactgggcac aagccaagat atttttattg gtttggctag
    24061 tgacaatctt gtgctgttct tcgttgattt ccttgatatc caaaagaacc aagtcagtga
    24121 cagccatgag tttgtcaaac ttctcaaggt aacgcggttt attacggaaa ggaagagcac
    24181 aggtgtccaa ggtacagtgg attccttgtt ccttagcctt ggtgaagaga gcaatcagga
    24241 aatcaatctg caagagagct tctcctccac tgactgtaat cccaccctta tttccccaga
    24301 aaccacggta gcgcaaggcc tctgtcaaga catcatctac cgtccgttca cgtgacttat
    24361 tggactccat agcccaagtg tctgggttgt ggcaatactg gcaacgcatg tgacagccct
    24421 gcaaaaagac aataaagcga ataccaggcc catctactga cccaaagctt tctgtcgaat
    24481 gcaccatccc tgtcacttgt ccataatcaa ttgtttcttc agacatatcg ttaccttcct
    24541 tgaaaacgtt ttatagtttt attatatcac gacttgaagg aaaaacaaga tttttgccta
    24601 ttttttagaa agcaatctgt ttttcaattt cattttcaat ctatcatcag attattcttc
    24661 aaaatcaaag ccatataagg ttgcaaaata gtcctcgggg cgctctgcac ggcggatttg
    24721 acgggcttta ccttcttcgg tatagaggat ttccgcagaa cgtaatttgg cattatactg
    24781 gtagcccatt gaaaatccgt gggcacctgt atcatgaatt accagcaaat caccgatttc
    24841 tgtatgaggc agttcgcgat tgactgcaaa tttatcattg ttttcacaga gtgaaccaac
    24901 cacatctacc gcttcagctg gtccatctgg atgggtcacg ttgctaatat gatggtaagc
    24961 tccgtacata gctggacgca tgaggttgac tgctgaggca tccacaccta gataggtacg
    25021 gtaggtttcc ttcttatgag tgactcttgt gactagagca ccgtgaggtg ccagcataaa
    25081 acgacccaat tcggtgaaaa tcttgacctg accaagacct gctgacgtaa gaacttcttc
    25141 atacacctta cgaactccct caccaatcaa ggcgatatcg ttcggctcct ggtctggacg
    25201 ataattaaca ccaataccgc cagaaagatt gataaagtct agccaaatgc ccaacttttc
    25261 cttgatttca acagccagtt caaagagctg acgagccaac tctggataat agagatgggt
    25321 cacggtattg gacgctagga aggagtgaag cccaaaagtc ttggctcctt tttccttcaa
    25381 gatagcaaag gcttcaaaga gctggtcctt ggtcatgcca aacttagcct ccccaggatt
    25441 gtccataatg tctgtcccca gttcaaaaac gcctccagga ttataacgac aagagatgat
    25501 ttctggaatg cttgctactc tctccagatg ttcaatatct tcaaaggcat ccaagttaat
    25561 ggtcgcaccc aattcacgtg cataggcgta ttccttgtct ggcgtgttgt tggaagagaa
    25621 cataatctca gaacccagaa agtccagttt atggctcatc aaaagctcta cataactaga
    25681 gcagtccaca ccacaacctt cttcttggag aattttcaaa atagctggag ttggagtagc
    25741 cttaactgca aaatattcct taaagccctt gttccacgaa aaagcttggt tgacagctct
    25801 tgccttctca cgaatcccct tctcatcata caagtgaaag ggagtcggga actcggcaac
    25861 aatcgcttct aactcttctc tattgataaa tggtgttttc ataagcttcc ttctattctg
    25921 tttgcaaaga aaggctggga caatcgttcc aacctttagt tctatctctt atttatcttc
    25981 gtcaaagata ttgccaacct caacatcgat ggcttggttc ttgacatcaa tattagttac
    26041 cttcaagagt gacttgtagt caaactgctt ttcacgttct tcttgggcat tgtccgcaaa
    26101 gaccgctaca cctgccagtt ctgagtcgaa ctcgcgcaag agactaatca taccgttgac
    26161 cgttccgcca cctttcaaga agtcatccac aatcaagaca cggctgcctg ccttaagact
    26221 acgttttgaa aggaacattt tctcgatacg gtcaccactt gaacctgaaa catagttgac
    26281 gctaacagtt gaaccttcgg taattttcag gtcacggcgc acaatgacaa aagagacatt
    26341 gaggacattg gcaactgcat ttgcaagtgg cacaccctta gttgctacgg tcataaccgc
    26401 gtcaattttt tggtccataa agcttttggc aataatacga ccaatatttt tcaagatggc
    26461 tggtgtgcta agcaaatcag acagatagat ataaccacct ggcaagatac ggtcactttc
    26521 tgacaacttg gtacgcaagt cttcaaccat ttccttagca tcctgactcg aaatagacgg
    26581 tgtgaagatg acacctccgc cagccccagt cactgtctgg atatgaccga tttcaatttc
    26641 ctcaaaggcg cgtttgataa tgacgatatc ttctgagatg gatgatttag cagactcata
    26701 cttttcagca aaagtattga gactagttag tttataagga ttattaatca aatagttgga
    26761 aatgacaacc atccgatcac ttcttcttaa tttcatttct atttcctatt ccatttttat
    26821 tcaaaaaatc aaaaagcaaa ctagaaagct ggtcgctggt ggttcaaaac actgttttga
    26881 gattgtcaat agaactgaca aaccctgtaa tatacctgca tatatacata cgacaaggcg
    26941 atactaccct agtttgaaga gattttcgaa gagtattcat ttttgtcttt tacttattat
    27001 accatattca cataaaaaaa cgaacattct tatcctaaaa aatgctcatt tttcttaaat
    27061 tattaatcta aatctggttt atagaaggaa cgattatcca tagcgaagat tttattggac
    27121 atctctcctt tatccaccaa agccagagct gttgacatca tcatcatgct tgcatccaga
    27181 ttgtcaatca tatggataat ctctgcctcc ataatacgtg gacggactgg acttccatat
    27241 tcaagcaagc cgtggtgact gaggatgaca tgacgaagca aaacgacttc ttccttggta
    27301 tcatcgatgc cgagttccat aactgtcttg gtaatttcgc tatcaatcag agcgatatgt
    27361 ccaagaagat tgcctctgac cgtgtactcg gtctggtctg gtcccgtcaa ctcgatgacc
    27421 ttagccaagt cgtgcaacat aatccccgca tagagcaggc tcttattgag ctgaggataa
    27481 acttcgctaa tagcgtctgc caaacgcacc atggtcgccg tatgataggc caagcccgtt
    27541 tcaaaggcat ggtggttggt cttggcagct ggataggagt agaattcctt atcatacttg
    27601 gtgtagagat ttcggacaat ccgttgccag acaggatttt caattttgaa aatcatttgc
    27661 gacatgtagt cacgaatttc cttgacatca actggtgact tgaccttgaa atcagctggg
    27721 tcattgggtt caccagcttg aggcaggcgg agagtaattt gattgacttg aggggtattg
    27781 ttataaactt ctcggcgtcc tttcatgtgg acaaccttac ctgcggtaaa ggcctcaatg
    27841 ttatgaggtt gggcatccca gagcttccca tcaatctcgc cactatcatc ttggaaggta
    27901 aaggctaggt agtttttccc agctcgagtt tgcctcaggt cagctgattt gattaggtaa
    27961 aagccttcaa atagctcatc ttttttcatg tgactaatct tcatattctt cctcattttc
    28021 ttgaaaatgg agtagatcaa gcgcaggctc gccttctgac aactcaatgt gacggagcgt
    28081 ccgctcgata gctatggtac gacggtttaa taattcatca atattgccag aggcatgttg
    28141 gagatgtttt tgtgccttga ccagaatacc accaaacttg ccaaactcgg tcttgacact
    28201 ggcaagagtc ttgctgatat ggtcggcact cttttggata ttaagggtct tgaaaccaac
    28261 tgatagggag ttaagaaggg ctgatagggt acttggtcct gcaacgataa tctgttcttc
    28321 ccgtctcaaa tcatcaaaga agaccggatt gcgcacaatc tctgaataaa gaccttctgt
    28381 cggaacaaac aagattccaa aattggttgt ccgaggcggt gctatgtact tgctcttgat
    28441 atccttggca aagcgcttga cgcttgctag gagtgactta cgacagcgtt caatctcatc
    28501 cttgtcacct gtctcatagg cttcttccaa gcggtaataa tctgccagtg gaaacttaga
    28561 gtcaattggc agatagacgt attcttggtc gccttgtccg ggtaacttga tggcatactc
    28621 cactcgttca ctagagtttt caaccgttgc gtattctcgt tcgtactggg caggtgtcat
    28681 gatgtcttca ataatttgcc ccagttgcaa ttctcccaga atccctcgcg tcttggttcc
    28741 agagagaacc ttgttaagag ctccgacatc acgggcaact gtctgcattt ctccaagacc
    28801 acgattgaca gactccagtt gtttagaaac tgtctcaaag gaagcctgta agcgtgtctg
    28861 caaggtcttt tctagttttt cctcgaccgt ctggcgcatt tgttccaaac gttgctcatt
    28921 tgattcctgc aaggcttgga gacgttggtc tgtcttgtct ctagtttgga ggagattatc
    28981 tgtcatttct tgacggactt gggtcaggcc ttggtgcaat tcaatccgca cttcttgcaa
    29041 acggtcgctg acagccactt ccaaatcttt ttggtctaac tggctggctt gtctggcttg
    29101 gtcaaagcgg taatccaact ggtctgacaa atgatctgcc tgatcctcca agctcttact
    29161 taagtgtttc tcctgcctat cctgcctttg ccaaatcaga aagagaccag ctaggttggc
    29221 aattaataat agaataagta aactttccat cctacctcct gtccttgcta tgcagtacga
    29281 ccacataacc atctgggcaa gttaccgaca cttccctatc tatatattcg ttagaagcgt
    29341 acactttttt aaagaaaaaa ttctcctctg tcaactcata cttagctcca agaatagtca
    29401 gctggctatc ccgaactggc ataaaggcta gatagtcgta gtctgaacga ggttctagct
    29461 gactggtccc ttctgaacaa taggcaatca aattttgccc atcctcaatt tctatttgac
    29521 gcatataggg tgccaactta ggattgctag gtagaaagac attggccaac atatggtcaa
    29581 tacggccacc caaggcaccg aaaatagtga cctgagcctg aggattttgt tcaaagatgg
    29641 ttaagagagc caattccaga tctgtatcat ccttttctgg tcgtgcttgg acaaaatact
    29701 gggcacgttt ttgaatcacc tgtcgctctt cttccgtcac agaatcaaaa tctccgactg
    29761 ctagagcaag aggtaagtct tcttccaaga cccagagcga gcctcgatcc accccaacaa
    29821 aagcatcaaa atctgtccga taatgaccgc ggtttccgcc tgcaaaaact gcaaccctag
    29881 tccagttgtt ttctgagagt ttgtactcgc tcattgactt ctcccttaaa gacataggaa
    29941 cctgctacaa aaacagtcgc accagcttct ttggcttgag caatagtttg gtcatcaatc
    30001 ccaccatcca cttcgatttc aaagttcaaa cctttttcct cacgaagagc aaccaactca
    30061 cgaaccttat ccatggtttc tggcagaaag gcttgcccac caaaacctgg attaaccgtc
    30121 atgactaaaa cttggtcaac tagatgaagg acgtgcttga tggcttcaac tggtgtgcca
    30181 ggattgataa cgactgaagg cttaactccg agtgaacgaa ttttttggag ggcgccatga
    30241 atatgaggtg ttgcttctac atggatactg atgatgtctg cacctgcacg cgcaaaatct
    30301 tccagatgat gctcagggtt tgacaccatc aagtggcaat cgaaaaccat cttactatga
    30361 ggacgaaggc tctcgaccac acctgcacca aaactgattt gcggtacaaa atgactgtcc
    30421 atgatatcga tatgggcata ttctgcccca gttgcttcta gacgtttgat ttcacgttca
    30481 aagttggcat aatctgctgc cagaattgac ggagcaatct tgtattgaga cataggtttc
    30541 tccttatttt ggaatttttt tgctgacttt tttataggtt tctctacgat tttcaatttc
    30601 actaaggaat tgcaggtaat tgtcaaaacg gaaggttgca ataacaccct cttcaacagc
    30661 tggtttgacg gcacaagacg gctcatgggt atgggtacaa gtacggaact tacaatctcg
    30721 gctaacagta gcaatctctg ggaaagcctg attgaggtct tcagcccttg atacttcata
    30781 gtccaaggat gaaaatcctg gtgtatctgc gattttaccc ccgttgagat tgtaaaaact
    30841 aacagctcga gtggtatggc gaccgcgacc tagactgtct gaaatttctc ccgtttcaag
    30901 attgaggtct ggtgcgattt tattgagaag agttgacttc ccaacacctg tctgccccat
    30961 aaagaccgta accttgcctg ttaacaaaga caggagttcc tctttactgg tcacaaagtc
    31021 atagccgatg tcaccatagg tctgctggta aaaatccagt tctcccctat cttccaacaa
    31081 atccattttg gaaatataga caatgggatg gatgcccttg tgctccaaaa gaaccaagaa
    31141 acgatccagc aaattgctgt taaaatcagg ttccttgacg gacatgatta ctacagcttg
    31201 atcgatattg acaataggcg gacgaaccag actgtttttc cgttcgtgaa ttttgaggat
    31261 atagccttct gaattttcct cggcagagaa atctacccag tccccaacat aagggatatg
    31321 gcctttttta cggaaattcc cacgcgcgcg tgtttgataa acctggccat cactctccac
    31381 atagtagaaa cctgccaagg ctttaatgat ttgtccctgc atcttagagt ccttgccctt
    31441 taagtgcgtc tgctagaccg gcaaattctg ccaagctgag agcttcccca cgcacacttg
    31501 gtgacaagcc tgcctggtcc aaagccttgg tcagcttgtc cttgacctct tcagtcttac
    31561 caaagtaacc tgtcaagtta ttccacaagg tcttgcggcg atgggtaaaa ctagccttgg
    31621 aaaccttaaa gaaaaagttc tcatcttcta ctgctacggc tggctctgga cgacgcacca
    31681 ttttcaagat ggctgaatcc acatttggcg ctggcacaaa gaccgtacga ggcacgataa
    31741 aggcaacctt ggctgtcatg taatactgca cggcgataga caagctaccg taagccttgg
    31801 tgttaggctg ggctgaaatg cggtccgcta cttctttctg catcatgacc acaaactcac
    31861 taaaaggaat gccactctca atcaagtgca tgagaatagg cgtcgtgatg tagtaaggca
    31921 aattagccac taccttgatt ggcaggttag gatttttaaa attttggata tgttgcgcca
    31981 aatcaacctt gagaatatct tcgttaacta cggtcacatt atcaaaatca cgcagggtat
    32041 ctgccaaaat tggcaccaaa cggtggtcaa tctcaaaagc catgacttgg gctgcacgct
    32101 cagccaaaaa ttctgtcaag gcaccaatac ctggcccgat ttcgatgaca ttgacctgat
    32161 catcaatttc agccgtatcc acaatttttt gaaggatatt ggtatccgtc aggaaatttt
    32221 gcccgaagga ctttttaaag gtaaaaccgt gacgctccag cactgccttg gtcacgctat
    32281 aatctgcaat tctcatctat tctcttttct attatctgtt actactattg taacacattc
    32341 cccttgtatt ttgggaatga aaatcatacc aaaaaactat ctttcctcat gaagaaagat
    32401 agtcttggaa atctttaaaa gtggccttag cagttctctc gaaacatgcg ttcaaaaaaa
    32461 tcagcttctt gataacttcg attttcgggt agggtcaaat ccacatcctc ctccacatca
    32521 tccactgtca gttcaaccgt ctgaggtaaa tttttcaaac gataatgctt ttgcctttgc
    32581 ccttcaatac tagcaaaatc aagcaaatag ccatgcactc ggatttgaac caaaaggaga
    32641 tgattggtca tattttccat gatgaggata tcttctaaac cacctctggc actaggataa
    32701 tcagataccc tgcgaatacg gattttttca cacatgagca agtttcctca tatcaatgat
    32761 ttcatcagcc ttattccaga ctaggggatt atgggtcgca atgatgataa ttcgattttc
    32821 atctttcaaa tccaccaaga gattcataac ctcctctgaa ttttcaggat caagagctgc
    32881 tgttggttca tctgccaaaa tcaagggtgg atttttcaaa atagtcttag caagggcaac
    32941 tcgttgggcc tctcccccag ataaagtata gattttttgt tctaaatcca aataccctag
    33001 attaactttt tctaaagccc ccacttgcct ttccaaacgt tctacttttg agattttctg
    33061 accaacaaaa cccaaatcca aattttcttt gattgattgg ttttctaaga ggccgaaatt
    33121 ttgaaagaga tagcccatct ggtctcgaaa atactcacga gtgggaatgg tttttaaatc
    33181 tttcccctga tagagaaccc ttccaccatc tatcttttct agctttccca agatattcag
    33241 cagcgtcgtc tttccacttc cactctttcc gattaaggca taaaccttgc ccttctccag
    33301 cttgagattc aaaccagaga aaatcgcgcg gtcattaaat ttcttttcca atccttgaat
    33361 atcaatcatc ttattttcct ttcaatactg ccatgctacc agcttcttct tttttgtcct
    33421 gccttactaa aatcaagagg gcgttaagcg taaacaaagc tacaactaga gcgctaatca
    33481 aaccatctct tgtcaaaata ctagatagga ctaggccaag caagagaact cctccttgcg
    33541 ccagtaaata cttgccatga agctcataga ttgtcatacc agtaagacgt ttaatcataa
    33601 gatcccgtct gaactgctca aaatagagaa gattcatgga atcaaataac aagatagccg
    33661 tagacagggt caaaatcgtc ccaataagga gagagtaaaa ctccacccgt ttgtcattta
    33721 gtaccttggc aaaaaatagc tggcttttta ccaagtaaga aaccttgtga tacagacctt
    33781 gctcttttag agctgtgatg ctgtctccat atcgatctag tttcaaggca ctattagccg
    33841 tagttcccca caacatccct gcaacaggtc ttgctccagt cgcttgcggc gttagaacta
    33901 caataatcgg atcttttagg tactggcgtg ttgtcttgta cccatcatta tagaggaaac
    33961 gttcctgtcc tgtttctgta aaagctagcc ttacctgtgg gaggtagtgt ttctgactcg
    34021 tcacttctac actttcagat gaaaagtttt gcaggtaatc tgtaaacaat ccttggtagt
    34081 agacagactg ctctcgcaag ctctcaggca agatcagccc aaactctccc tcagacaagt
    34141 tttgcatctt gtccataaac tctgaagaaa cggtaatctt ttcttctatc agatagcgtg
    34201 gtgagacata gataacattc cctgacggtg tgtagtcact gagacgattg ccatctaggt
    34261 ccacttctgc tccatctgag aaattgtcaa cattgctcat aatataaaaa gagtctgtat
    34321 tggctaaccg ttcttcagta aatgtctgcc actcacgatt atccttacgc gttccttctt
    34381 catcggcaaa tgcactagac caaccaaagg atagacggta acggtctgag gactggctcc
    34441 atttattgct agctctctcc atttcctgca tttcacggta gtggggtagg agagctgtcg
    34501 cactcgatcc gaccaccaat acagctaaga gttgccccac catcatcaat gtcatcatac
    34561 gtttgagagg gagtttccct ttcaatagat ccaccagact attttcctgt aaaccaagta
    34621 gatagacgac acttagtaag gtagaaatcc ctgccaaggt caatccataa agtagaagag
    34681 caatgatgac cagttgcacc gttgccataa acaaggcacc ttgataccag agaatcccca
    34741 atcccaaaag actggatacc agcactgagc agataagctg tctcacatct tctaacactg
    34801 gtctgagagc aactccaaac aagctctcac cagctattaa gcgaatccct gcctgacgaa
    34861 gggatttgat ccgataaatc agggtcagac tcataaaggt cagcagaaaa atagctaaac
    34921 tcagtagcac cataggggtg gccgtcaata gtaagactat cgaaaatgga tcttctccat
    34981 tcgaaacaaa gccttgataa ccaagctctt tcaaggtggt ctgtaagctc actccatcca
    35041 aacttcctga cacaatcaag taagacccta ataaatcact cgtttctgca ctctccttgg
    35101 aggaaatggt cagaccttct ggaagctttc cctcaccata agtggcatag gtaaagcgtg
    35161 tttctccagc ttcatttggc tcaacgattc gacgggcgat gagactatta tgctctaccg
    35221 ccaagtgact caaagcctct gtcacctctt cgcgactagc tactggttga tttgtcatca
    35281 ttcctgtcac atcaacagaa ggatagttac caaataaaat ctcttctcga tggtcaacgg
    35341 aagttatcac catataaagc gataccaata ccattgaaat caaaataaat aaacgtttca
    35401 tcatttcctc caaaaagtta cttggtcact tagtgagaag gtgaccacag tcactgcgat
    35461 gttaagaagc ttattaagtt ctttctgcta gcaacatctt caaatacatt atatccgttt
    35521 tttcaagaac tatcaaggac atcaatgtcc ttaaaatcta actatttcat attattttta
    35581 gcttatatct aaaaaaacag ttctaaagac gaactttaga actgttttga tacgaggaca
    35641 aggacatttt tgtcactgat tcactatgtt tataaatatt ttttgacatc ctcttgccat
    35701 tcctctgtca atttctttac tagaaatcca tctccaatta gcttcgcaaa tgttttagaa
    35761 gactgataaa atgattcagc taaactaaaa tctctttcta ctcttaatgc ttgcttccac
    35821 aaaaacataa atacaagagg tttcttttga taatcttgag ttttatccat aatttcttgt
    35881 aaacacgata gataatcatt aaaacaatca gttactccta ccttttcaag acaacacatt
    35941 cctgaaataa gagtatctct aattataaag taattctcta tttgagttac cttcttctgt
    36001 tccaaaagaa agagcattag cttttcgatt tgatttataa atttggcaag atcttttcta
    36061 atgagcattt gataaaaaaa caaacgaatc aacaacatat catttattga ataagcttct
    36121 ttttctataa tctgaggcat atgttcctca agtattaact ctgcatattc aggccttcta
    36181 gttctagaag tatccaaaca cgcctgcaat acttcacatg ctaattgttc ttctttagga
    36241 agattatcaa agtatgtttt ataaatctct tccaaacacg cttccttctt atcgtactcc
    36301 tcttctttac catagattgg ttcacgtaaa atctggtatt tcaattctaa ataagcagaa
    36361 ggaagagctg aaaaatccgg cataaggcta taaactggaa ctcctagccg gcgagcaata
    36421 taggctaact tgggcaaact cggttgcgaa gctccacttt caattcgcga cagttgacga
    36481 actgtcagtc cttgctcatc tccacaaaaa tctggacgac taattccttt ttctatccga
    36541 atctctttaa ttttttgacc aattaacatc agacgcctcc tcttctacct gcttcgaaaa
    36601 atactgtgaa acttaaaaaa ttgtaacggt cacatctttc ctactcataa gatttcatag
    36661 cttcttccac ttctgccaaa gtaaccccaa acaactctag gcgtttgagg agttgcttgc
    36721 cgttagaata gccgattcgg agagtctctc cgagatattc tctacgctta cggctgtctg
    36781 cccctgctag aaaaccaagg cgaatcaaat cgctacgact aatgtcaaac tgactctcat
    36841 gttcaaattg ttctgtcacc tgagctagag ccgttttcag gtcttcatag ctggcatgct
    36901 caattcccag agaacgcccc ttggtcttgg acttgggaac agcttcatct cgcttgagaa
    36961 aggcatgctg aactgttgga atggccgtca taatcatgcg ccgaatccgc tccccattaa
    37021 aatctgggtc tgtaaagaca atgactccat gacgttggtg tagacgctga atccgctcta
    37081 tgtcctggtc attgatggca gaacctcgag tctcataggt ctccacatcg aaataacgct
    37141 tgagattgac cgtatcatca cgaccttcaa ccacgataac ttgggaaatt ctctctttca
    37201 ttacttgctg tccaatccaa aaatgcgttc tgcatttgca gtcgttgcta ccgccagctc
    37261 ttctgtcgtc ataccacgca agtcagcgat aaagtcgacc acatagcgag tataggctgt
    37321 tttattttca cgaccacgct tgggtacagg tgctaagtaa ggcgcatctg tttctaccaa
    37381 catcttgtcc aaaggtaact ctttagctgc ttcttggagg tcagttgcct tcttgaaggt
    37441 caccactcct gagaaggaaa tggtcatacc aagatccaca aacttctctg cccactcaag
    37501 cgtccctgaa aatgaatgca tgataccacc acgaggacca acgccctcac tcttgataat
    37561 ctcataggta tcttccagcg catcacgggt atggacaaca aaaggcaaat ccaagtcctt
    37621 agatagctga atctgacggc gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca
    37681 atggtagtct aagccaattt cacctaaagc cacaaccttg gaatgtttta acttatccaa
    37741 caagtaagcc tcaacttcct ctgtataagt accagcttct gtaggatgcc aaccaatagt
    37801 cgcatagagc tgctcatact catctaccaa ctccaaggca tgctcaatcg tcggtttatc
    37861 aaaaccaaca atattcatct gtgtcacacc catctcagca gccaaggcaa tttcttctgc
    37921 ctcacgacct gcaaattctt ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc
    37981 taacctcgtt ttctatcctt ctattatacc aaaaaagagg aggggcacct aatttttcgg
    38041 tttcccctcc tctcttcaat agagagctat tctgctatct tttctatccg atattgccca
    38101 tctcctattc cacagttaga gacagaagag attggctaca ttgatagaat attcctattc
    38161 agctaaagta tgtgattatc ttgggttctc acttttctca cccatcacta ggattgatga
    38221 cttctcaaaa tcccccttat aatctaactt taagtagaga atagaatttt ctagctgttc
    38281 tattttctac gtctagtctt tcttcctagc aaagcaaaac cacttgttgc caccactaga
    38341 ccaagactag caagtgctgc acttgatgct gaacctgtgt taggtaattg tttgccagca
    38401 ggtgactggt aggtaacatc ttgcattgtt cctgcagttg cttgcggtgc acttgttgtc
    38461 ttaggagcag cattaggagc cactggtctc ttaggaactg taaccatggc aatggcttgc
    38521 actccaatcg tttctgcttc cttcacatct tccatagttt tcgcattttc aatctctttg
    38581 agagctgctt gtttttctgc ttccactctt gctaaaagtt ctgctttttc tttatcagaa
    38641 agcggtgcgc ctttgatttc atcctgcttg ttcttggctg ctttttcaat ggcattttta
    38701 gctgattctt tttcagtagc taattgctta gaagcttgtg attgagctga gttttgtgaa
    38761 cctgtgtgag ctgatccacc cgcctggcta ccaccgcctt cattcgcatt agcatctgag
    38821 cttccagtat tgcctccctt atctccacct ggtgtatttt ctagcttata ctctggtgta
    38881 gcttcttgag ttacagattc acctttcgca gattgttgaa ctgtatcttt tcctagaatc
    38941 gtcactactg aaccatctgg gaatgtgatt gtcgctgtgc catctccagc tacttcaact
    39001 ttatagtaaa ataaaataat aacaggacaa atcgatcagg acagtcaaat cgatttctaa
    39061 caatgtttta gaaacagagg tgtactattc tagtttcaat ctactataac atcgcttccg
    39121 aatccatttg catctactga acttttttac aaaaataaaa atcagtagac tcgagttcct
    39181 actgattttt gatagaattc gtttggatta tgcagccaaa actttgcgtc ctttacgacg
    39241 acgagctgcc aatacgcgac gaccattttt agttgacata cggttacgga atccgtgttt
    39301 gcgcgcacga cgaagtttac ttggttgata agtacgtttc acgatgaata cctcctcata
    39361 gattttgtat tcgtttagcc ggctataaag tgatcagtta ctaaacatac tttactattc
    39421 tatctgattc tttcactttt gtcaatagcc ctaggcaaat gtttccagct ttttcgaatg
    39481 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
    39541 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
    39601 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
    39661 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
    39721 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
    39781 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
    39841 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
    39901 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
    39961 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
    40021 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
    40081 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
    40141 cttaggataa agggctccag ttgggttatt tggattgata aggacgattg ccttggtatt
    40201 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattcaacag cttcatcaca
    40261 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
    40321 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
    40381 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
    40441 cagctggcaa tactgcatga tggccttacg ggctgagaaa attcctttgg agtcagaata
    40501 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
    40561 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
    40621 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
    40681 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
    40741 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
    40801 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggt attttgcaaa gacacatcac
    40861 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
    40921 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
    40981 aataagatta gatggtagag tacatgaaaa actaaaaatc caataaaact tggataaaga
    41041 agcggtacca gactctctaa ttgacagatc atgatcactt ctaccaagac tgaaactagg
    41101 ataattccat aataaaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
    41161 ttcacttctt tcctgctatc ctttctttta ttttatcata tatttatata gagtttaagc
    41221 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
    41281 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
    41341 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
    41401 acgaaacgac gctaaactca ccatcgtaca tgtcattgac acacgcgctc tccaaagcgt
    41461 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
    41521 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
    41581 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
    41641 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttagtcggct cttcatctga
    41701 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
    41761 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
    41821 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
    41881 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
    41941 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
    42001 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
    42061 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
    42121 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
    42181 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
    42241 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
    42301 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
    42361 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
    42421 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
    42481 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
    42541 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
    42601 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
    42661 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
    42721 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
    42781 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
    42841 gacatgattt tagaacccaa catggcatcc ttggtcccta gagcaatctc cgtgccctct
    42901 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
    42961 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
    43021 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
    43081 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
    43141 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
    43201 ccccttttgt gatacaatga ttataccaca tttcagaaag agttagtaaa tcatgcctaa
    43261 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
    43321 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
    43381 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
    43441 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg tgatcacaca
    43501 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
    43561 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
    43621 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
    43681 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
    43741 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
    43801 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
    43861 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
    43921 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
    43981 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
    44041 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
    44101 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
    44161 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
    44221 ttcgaaaatc tctgcaaacc acgtcagcgt cgccttaccg tatgtatggt tactgacttc
    44281 gtcagtttca tctacaacct caaaaacatg ttttgagctg acttcgtcag tttcatccac
    44341 aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc ctagtccgct ctatggtatt
    44401 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
    44461 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
    44521 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatagc
    44581 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
    44641 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ctttatcagc
    44701 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
    44761 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
    44821 taccaaaccc tcactataag tgattcctgc ttttttcaag gtttccttgt agccaactaa
    44881 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
    44941 ttctttagca aggtaactca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
    45001 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
    45061 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
    45121 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
    45181 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
    45241 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
    45301 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
    45361 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
    45421 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
    45481 ttttcctgtc ctttctatct cacatattct tttacaagta gaggtgctga ttgaagctct
    45541 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
    45601 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
    45661 agaaaaccta ggtttagctc cttgctacca ccttagacta aacaaaaagg aggaaactaa
    45721 gccctcctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
    45781 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata
    45841 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcataaaa atattatatc
    45901 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
    45961 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
    46021 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
    46081 ctgtcaacgg attgggacgg gtcatcacca cctccatcaa ttcaggcgaa tactccttgc
    46141 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
    46201 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
    46261 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
    46321 ctgtcttaac aggcatttct acgtcaagga tggtgatatc tacagactcc ttttctaata
    46381 gttggattgc ttcttgccca ttcttggctt gaaggacaga ctccacatct ggttgaagcg
    46441 tgagcaattg acacatggca tctcgcaaca tactttgatc ttctgcgact aatactttca
    46501 tcttagtttc tctccttata aggtagtcga acttgcactt ccgttggatg tttctgactg
    46561 attacactta cttctcctga aaatggaaaa acacgatctc ggactgtatg gagctcatct
    46621 ccttttagaa aagcaaagcc gcagccatca tcacttactg ttaaaatgag ttccttctct
    46681 gtccgttcta attttaagta agctttagac gctttggcat gtttgatgat attggtcacc
    46741 aactcaagca aaatcataga agccattgac tccaattctt gagtaaggct agcagtatct
    46801 agttggttat ccgtttccac ctcaattcca gcaatttcta acatcttttt cacagtctct
    46861 agttcggatg tcaaagttct agacttaaga ttttccacaa tggttcgaac ttcacgcatt
    46921 gattctttgc taatttgctg tatttctctt aattcctttt ccacttgtgg ataagcctgc
    46981 atttgaaata gctgcaaggc tagatctgtc ttgacactaa gcatagcaaa ggtatgtccc
    47041 agactatcat gcaaatcctg accgatacga ttgcgctcat tttctgctag caatagattt
    47101 atctgggtat tttgcttgac ctgagcttct ttcaaatcct cgacaaggcg aattcgaacc
    47161 gagcccaaag tcatcaaatc gacaaaagta agaattacaa gcatatagac tagaaactca
    47221 acttcgattc tctgaaaaat caaaagtcgc cccacaacaa ggacttgagc aaggagaaat
    47281 gtccatacat gtaaagactt taaactacgt acgccaaaat gataaattaa gagattggaa
    47341 aggaaaaaga aaaaccagat ataattccca gcaacaaagg cggtattccc agctacatag
    47401 accagcatga gcccccagaa aagccaagat aggcgctggt tcttagttat taaaacaccc
    47461 aaatacgtca ctacaaatag aatatcaacc aataaatgcc aagcaggaag ctccccaatc
    47521 actacaggta ggatgggaaa aagcataaaa atcaaactgg cccaaaacat atagtgtatg
    47581 cttttcagtc tttcaagcat taagcattct ccttatgacc ttgaaggtaa atagtcaaac
    47641 caaataaaac tgctgaaaaa acaagtaaat aaactgtggc tgatagattg atgccaccct
    47701 cgtttaagaa ggtcttgagc aactccatca attgataggt cgggagacac ttaccaactg
    47761 cttgcatcca gtctggaaat aaagaaatgg gcatccagag tccgcctaaa acagccaatc
    47821 ctagataaag aagattgccc acaacagaca tcagctgact agttggtaag agagtcaagg
    47881 tcaagccaag tgctacaaaa gcaatactcc ccacaatcag caaaatcgca gctccaaccc
    47941 attttactag aggcatgtct acacctctta caaaatgtcc aactgtaaaa acaaccagaa
    48001 ttgagaccaa ataatcaacc agcatacttg ttatctttga tagataatat tctaccatat
    48061 ttacagggct atgacgcaat gttttctgcc agttgttcat cttatcggta tgtaaaacag
    48121 ctggaaatga gaggatagtt gtcgacatca tggaaaaggc agtcatggag atgaggtaat
    48181 cacgcataaa attcgcgagt tcacctggtg tgtcctgata gataccagaa aaaaataaat
    48241 agaaagccgt cggcatccct accgacaata gataatagat taattgtcgt ttggtcaata
    48301 aaaattctat cttactaagt gctatccatc gtttcatctt agttatctcc cttctgcgtc
    48361 tcttcaaaga ttgtattcaa caaactacga ttattaactt cgatttcttg catcctacat
    48421 cctgcttggg ctaatagttc ccagaaagca tctgcttctc gagtgactac ttgtaaagaa
    48481 tcttgcttta gggtccagtt ttcaaccaaa tttgactgct ctacgacttc cttgtaagct
    48541 ataggaagga taaagtgctt ttctatttcc tcgctacgca tagctagagg cgtcgtatct
    48601 cgaatcaact ctcccttatt taagagcaaa atgcggtctg cagtgtgttc cacttcctca
    48661 atataatggg acgaatagag aatggtgact ccttgcgctt ttaactcctg gacaatttcc
    48721 caaaaacgtt gacgagttga ggtatccatg gacgcagtag gctcatctaa aaaaacaatc
    48781 tttggtcgcc caattaaggt caagacgaaa gaaaagagac gcttttgacc gcctgataat
    48841 ttttctgcca actgttcttt ttgttgcttg acaaactgca atagttgatc aatttcctga
    48901 ttgctcaaag gatttggata gatacgttga aagaaagcaa tcaactcttt aacttttaat
    48961 ttctgaacga ttacattttc ttgagggagg taagccctag tatagtctaa cttagaactc
    49021 gtcactggta aaccttgaat agatacttga ccgcttgtga ccagtttatc tccaagcaaa
    49081 caatctaaga gtgttgtctt tccggcacca tttggaccaa ttaaggcaac acattcacca
    49141 tcgttgattt caaaggagat attccgcaaa atttccttgt cttttatttt cttactcaat
    49201 ttctcaactt taaccaccgt catgagactc tcctttcaac cactccattc ccataaggaa
    49261 aacgacgaaa atcataaatc caaaccccaa agcaccacga atgaattggc gaagcaaggc
    49321 ttgatcaaac caatcagtaa acatttccac taaccatacc aagagtgaca ggccgataaa
    49381 gaaatagatg atccctctct tcattcctca aactcctttt tcacatctct gactaatttc
    49441 aaaccttctc taacaagcca agacatgatt ccaaagccag caaagacctt ccaaggaaaa
    49501 tggtagaaat cctcatccaa tcccgaaaac atgagatagg tcataactcc tgctactact
    49561 aaacttactg cgataatcat tttatttctc atctcttctt cccccatttc atactacaat
    49621 tatagtcttt taaaatcaga ggagacagaa gcttctgtca ctagaaaata tgacaaatgt
    49681 cataaaaaat tctgttcaaa acaagcaaga tacactatac aataaaacac aatcagaaaa
    49741 atctaaggca acttcctcaa aagagatatc aaacccaatt cacaccataa tgtaaactaa
    49801 tacttattta aaatcaaaaa gagtaaaaat ttttatcaga caaacacata tatag
//
LOCUS       DAVKJU010000004        48150 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVKJU010000004 DAVKJU010000000
VERSION     DAVKJU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.004"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            145..6846
                     /locus_tag="VW191_000178"
     CDS             145..6846
                     /locus_tag="VW191_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU9623028.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEVPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSDLPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7094..7950)
                     /locus_tag="VW191_000179"
                     /pseudo
     CDS             complement(7094..7950)
                     /locus_tag="VW191_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8162..8521)
                     /locus_tag="VW191_000180"
     CDS             complement(8162..8521)
                     /locus_tag="VW191_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEU9623029.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8613..9569
                     /locus_tag="VW191_000181"
                     /pseudo
     CDS             8613..9569
                     /locus_tag="VW191_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9553..10110
                     /locus_tag="VW191_000182"
     CDS             9553..10110
                     /locus_tag="VW191_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9623030.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10103..10357
                     /locus_tag="VW191_000183"
     CDS             10103..10357
                     /locus_tag="VW191_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623031.1"
                     /translation="MDKQYLREKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10369..12423
                     /locus_tag="VW191_000184"
     CDS             10369..12423
                     /locus_tag="VW191_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU9623032.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWVALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12602..13462
                     /locus_tag="VW191_000185"
     CDS             12602..13462
                     /locus_tag="VW191_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU9623033.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13681..14391
                     /gene="ccdA2"
                     /locus_tag="VW191_000186"
     CDS             13681..14391
                     /gene="ccdA2"
                     /locus_tag="VW191_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9623034.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14411..14977
                     /locus_tag="VW191_000187"
     CDS             14411..14977
                     /locus_tag="VW191_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEU9623035.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14988..16100
                     /gene="msrB"
                     /locus_tag="VW191_000188"
     CDS             14988..16100
                     /gene="msrB"
                     /locus_tag="VW191_000188"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU9623036.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKTVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16368..17105
                     /locus_tag="VW191_000189"
     CDS             16368..17105
                     /locus_tag="VW191_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623037.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17102..18793
                     /locus_tag="VW191_000190"
     CDS             17102..18793
                     /locus_tag="VW191_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9623038.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFVQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            18909..19472
                     /locus_tag="VW191_000191"
     CDS             18909..19472
                     /locus_tag="VW191_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEU9623039.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            19494..24962
                     /locus_tag="VW191_000192"
     CDS             19494..24962
                     /locus_tag="VW191_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9623040.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAEAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAQPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEIKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKSAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNSKEDRGAEETPKQEDEQPAEAPE
                     IKVEEPVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVEVTTVETREEVIPFETKE
                     QEDDTLKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIEQPQDEIIKKG
                     TKGLEKPTLQWANTEKDVLKKSATASYTLTKPAGVEIKSIKLALKDKDGQLVKEVTVA
                     ENNLNATLDKLKYYQGYTLSTTMVYDRGEGEETEKLEDKQIQLDLKKVEIKNIKETSL
                     MNVDAEGNETDKSLLSEKPTDVSQLYLRVTTHDNKVTRLAVSSVEEVVVDGKTLYKVV
                     AKAPDLVQRRADDTLSEEYVHYFEKQLPKVNNVYYNFNELVKDMQANPTGEFKLGADL
                     NAANVKPNGKSYVTKPFKGKLLSNDGGRFTIHNIERPLFANIEGGKIHDINLANVNIN
                     MPWADKVAPIANVIKNNATIENVKVTGNVLGKDWVSGFIDKIDGSGKLINVAFIGNVT
                     SVGTGGNFLTGIVGENWKGYVERAYVDAHIKGKRAKAAGIAYWSQNQGNNFTIGSEGA
                     IKKSVVKGTIDVEKPIEVGGAVGSFTYHGSIEDTVSMMKVKNGEIFYGSKDIDDDPYY
                     TGNHVNRNYVVIGVSEGTSTYRYSNQHNRIKPITQSEADVKIAETAITADKFTITDPI
                     VNKLNALTTRDNEYRTTQDYEATREQAYRNIEKLQPFYNKEWIVNQGNKLVEGSNLLT
                     KEVLSVTGIKSGQFVTDLSDIDKIMIHYSDGAKEELNVTRQESNVQQVREYSITDLDI
                     LYTPNMVEKDRAQLMTDVKSKLSSVELESDGVRQLLVKRDTKKDANANSVGRQNGYIR
                     DLFLEESFSEVKANLDKLVKQILENEDHQLNDNELAERALLKKVEDNKAKIMMGLAYL
                     NQYYAFKYDELSIKDIMMFKPDFYGKTASVIDRLINIGSAENNLKGDRTQDAYRGIIS
                     GATGKGSLHDFLTYNMKLFTNETDINVWFKKAIEKNAYVVEQPSTNPAFANKKYRLYE
                     GINNGQHGRMILPLLNLKNAHLFMISTYNTISFSSFEKYGKDTDEKREKFKSEINKRA
                     KEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHNSPNGWASRHGHIDGKPDYAP
                     IREFFGRINKYHGYKYGYGAYAYIFAAPQPMDAVYFVMTDLISDFGTSAFTHETTHVN
                     DRMAYYGGHWHREGTDLEAFAQGMLQTPSVSNPNGEYGALGLNMAYERQNDGNQWYNT
                     NPNDLTTRAEIDNYMKGFNDTLMLLDYLEGEAVLNKANQDLNNAWFKKVDKQLRGAST
                     KNQYDKVRDLTADEKNITLNSVDDLVDNNFMTKHGPGNNVYDPTGFGTAYVTVPITAG
                     IYGGNTSEGAPGSMSFKHNTFRMWGYYGYEKGFLNYASNMLKNESRQAGHNTLGDDFI
                     IKKVSDNKFSTLEDWKKAYFKEVVDKAKAGFNPVTIDSTTYSSYDDLKNAFAAAVEKD
                     KATLKNGSVKSDNTVALKEKIYKKLLQQTDSFKTSIFR"
     gene            25023..26744
                     /gene="pabB"
                     /locus_tag="VW191_000193"
     CDS             25023..26744
                     /gene="pabB"
                     /locus_tag="VW191_000193"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEU9623041.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKNLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            26745..27362
                     /locus_tag="VW191_000194"
     CDS             26745..27362
                     /locus_tag="VW191_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9623042.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            27412..28410
                     /locus_tag="VW191_000195"
     CDS             27412..28410
                     /locus_tag="VW191_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9623043.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            28537..29496
                     /locus_tag="VW191_000196"
     CDS             28537..29496
                     /locus_tag="VW191_000196"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEU9623044.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTVGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            29593..30432
                     /locus_tag="VW191_000197"
     CDS             29593..30432
                     /locus_tag="VW191_000197"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU9623045.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRIDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(30480..30650)
                     /locus_tag="VW191_000198"
     CDS             complement(30480..30650)
                     /locus_tag="VW191_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEU9623046.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            30771..31655
                     /gene="miaA"
                     /locus_tag="VW191_000199"
     CDS             30771..31655
                     /gene="miaA"
                     /locus_tag="VW191_000199"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU9623047.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHFVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            31648..32886
                     /gene="hflX"
                     /locus_tag="VW191_000200"
     CDS             31648..32886
                     /gene="hflX"
                     /locus_tag="VW191_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEU9623048.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKFQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            32879..33502
                     /locus_tag="VW191_000201"
     CDS             32879..33502
                     /locus_tag="VW191_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU9623049.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLVQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            33517..34446
                     /locus_tag="VW191_000202"
     CDS             33517..34446
                     /locus_tag="VW191_000202"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEU9623050.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTVRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTNASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            34467..35222
                     /locus_tag="VW191_000203"
     CDS             34467..35222
                     /locus_tag="VW191_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9623051.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(35276..36184)
                     /locus_tag="VW191_000204"
     CDS             complement(35276..36184)
                     /locus_tag="VW191_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9623052.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLSHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            36248..36517
                     /locus_tag="VW191_000205"
     CDS             36248..36517
                     /locus_tag="VW191_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEU9623053.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            36517..36897
                     /locus_tag="VW191_000206"
     CDS             36517..36897
                     /locus_tag="VW191_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9623054.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            36986..37213
                     /locus_tag="VW191_000207"
     CDS             36986..37213
                     /locus_tag="VW191_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623055.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLEYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(37253..37978)
                     /locus_tag="VW191_000208"
     CDS             complement(37253..37978)
                     /locus_tag="VW191_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEU9623056.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            38140..39981
                     /gene="typA"
                     /locus_tag="VW191_000209"
     CDS             38140..39981
                     /gene="typA"
                     /locus_tag="VW191_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU9623057.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            40001..40255
                     /locus_tag="VW191_000210"
     CDS             40001..40255
                     /locus_tag="VW191_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEU9623058.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            40619..40918
                     /locus_tag="VW191_000211"
                     /pseudo
     CDS             40619..40918
                     /locus_tag="VW191_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            40989..43010
                     /locus_tag="VW191_000212"
     CDS             40989..43010
                     /locus_tag="VW191_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEU9623059.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            43012..43653
                     /locus_tag="VW191_000213"
     CDS             43012..43653
                     /locus_tag="VW191_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623060.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            43757..45109
                     /locus_tag="VW191_000214"
     CDS             43757..45109
                     /locus_tag="VW191_000214"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU9623061.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            45113..46171
                     /locus_tag="VW191_000215"
     CDS             45113..46171
                     /locus_tag="VW191_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU9623062.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            46181..47380
                     /gene="divIB"
                     /locus_tag="VW191_000216"
     CDS             46181..47380
                     /gene="divIB"
                     /locus_tag="VW191_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEU9623063.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     NQRSSRR"
BASE COUNT        14823 a         8837 c        10700 g        13790 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgctgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caagcttcaa gtcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ctttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgcagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatacgat atcaccaaat accttcataa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaat
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttat ggatcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg gaatgatcgt gttggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctacctcta ccaaagccaa
     2401 tgggtttctg ttaaaaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt gttcttgaat ggaaaatctc ttggtcttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagtcg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc acccttactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaag aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtgc caaaagtaca gaccattatt ggagaggcac ctgaaatgcc taccactgtt
     3181 ccgtttgtat acagtgatgg tagccgtgca gaacgtcctg taacctggtc ttcagtagat
     3241 gtgagcaagc ctggtattgt aacggtgaaa ggtatggctg acggacgaga agtagaagct
     3301 cgtgtagaag tgattgctct taaatcagag ctaccagttg tgaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcctatgttt tgactgatgg aagtgttgaa
     3421 gagtatgaag tggacaagtg ggagattgcc gaagaagata aagctaagtt agcaattcca
     3481 ggttctcgta ttcaagcgac cggttattta gaaggtcaac caattcatgc aacccttgtg
     3541 gtagaagaag gcaatcctgc ggcacctgca gtaccaactg taacggttgg tggtgaagct
     3601 gtcacaggtc ttactagtca aaaaccaatg caataccgca ctcttgctta tggagctaag
     3661 ttgccagaag tcacagcaag tgctaaaaat gcagctgtta cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggccc tcttcaaacc
     3781 tatgcaattc aattccttga agaagtgcca aaaattgctc acttgagctt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgat aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctgaac
     4021 gctgaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagtgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtcca
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaagg tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagactt gccacttgcc tttgcttcag actcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgtcc tattcggaga ttcaggtatt
     4741 ttgagcaaac gttcagttga taacttaagc gttggcttcc atgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtga gagcagacaa caaactagga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa gcaaggacaa
     5101 acaagaatcc aagttgacgg taaagactta gcaaacttca accctgatct gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagat
     5461 ttgtcagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcg
     5821 caagccaaac ttcacttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagcagat aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac caagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaataat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc acttgactaa ggaaaaacca gtttctgaga agacagttgc tgctgtacaa
     6421 gaagatcttc caaaactcga atttgttgaa aaagatttgg cctacaagac agttgagaaa
     6481 aaagattcaa cactgtatct aggtgaaact cgtgtagaac aagaaggaaa aactggtaaa
     6541 gaacgtatct ttacagcgat taatcctgat ggaagtaagg aagaaaaact ccgtgaagtg
     6601 gtagaagctc cgacagaccg catcgtcttg gttggaacca aaccagtagc tcaagaagct
     6661 aaaaaaccac aagtgtcaga aaaagcagat acaaaaccaa ttgattcaag tgaagctagt
     6721 caaactaata aagcccagtt accaaataca ggtagtgcgg caagccaagc agcagtagca
     6781 gcaggtttag ctcttctagg tttgagtgca ggattagtag ttactaaagg taaaaaagaa
     6841 gactagaaat tttgatacct tctttatcat taaagaccaa agaaaaactc ttgtcctaaa
     6901 tagaagataa agagtctgga acaaaatagt tctcaaccac aaaaaagcta gatatttcca
     6961 attgcggaac tctagctttt taattttgag tcgttctctt attgtatagt atattgaaac
     7021 tagaatagta cacttctact tctaaaacat tgttagaaat caatttgact gtccttatcg
     7081 atttgtcctg ttcttatttc atttcactat gttttaggag gttattggcc ataagtacca
     7141 atcccatgtc aattctcact tgacgcttcc ctctcagatt acatctcttg taacccaaac
     7201 aagcctttat ctgcccaaag acaggttcca catcaatctt gcgttgagcg aaaatctgtc
     7261 taccttgggg agataaaagc gcctgacatt ctttagcttt caagttttga tagcgttcgt
     7321 tcatatacag tcccttttga ggggctgatt caggttcatc agcgtagtaa accttgattt
     7381 cctgttgaaa gtctgtctgt gttttctgat gtttggtatg gtgaaaacga taataccagc
     7441 catcaggatg tgtgtagcta tcctccttgt cattatagtg ccaattcgct aagtttttag
     7501 ctgactcttt ataccctctc ttttgtttct tatcaaacat ggcatattta atcagaaggg
     7561 gagtgatttc ttcttgaaaa tagacctgta tctgttcttg aagtttggtg gagaatttct
     7621 ctgtcgtttt cttccacaca aaactatact tgttggcatt ggcttcaatc ttagttccgt
     7681 caataaacaa acaatctaag gtcactaact cttccatttt tcaatttttc gaccagagaa
     7741 aatccctttt gaataggcaa atagaagagt agatacaagc attttagggt gataagacgg
     7801 gcgatcaaag gcatgatagg aggtgtagaa gtgacgttcc tccaaggtat tcaccacttt
     7861 ttcaatagta aagacgagat ggtcttgtgg taagaaggaa ctgatttcta gtggtaaagt
     7921 tgtttgattt gtgttatagt gaatatgcat gggatagcct ttttaaatgg tttattaagc
     7981 aattctattt taccaagact atcgtaacca tgcaaaaaaa gcaacggcaa atccgttgct
     8041 ttttttggag ataagagact attgtcccag actcttttct ttgtataaaa ttgtatagtg
     8101 ttattgtcta tactattttg tagagaaagc aaaaatccct agaaaatgct tagggattcg
     8161 actatttcat taagaacaat tcaacaatca tttctatata catgataagg gttaataaga
     8221 atcctgcacg ccagaagaat ttgataaatt taggatagta aaagctgcgt tttttcaaaa
     8281 ggaagaaaaa gacgagaata atggctagga tcgagagggc tagccccagt ctagggagga
     8341 aattatgggt aaaggtttta gctgtaatca agtaatactc aaataccaag actggaaaag
     8401 ccaaatccgc aaagtttcgt cctagttttt ttaatctaaa aagtttggtt aggataatgc
     8461 agacgactaa ggtcagtatc aataataaaa tagatgctaa tttcattaaa atcataccca
     8521 tattgtatca taaaaaaagg ctaatggaaa cgagaaacag aggaatttat aggaaagtca
     8581 ggcttttgag attgtgggtg atttttgtta taatgaaagt catgaaatct tataatacct
     8641 tgaatgatta ttatcgaaaa ctctttggag aaaagacttt taaagtccct attgatgcgg
     8701 gatttgactg tcccaatcgt gatggaactg tggctcatgg aggcagtact ttttgtacgg
     8761 tttcaggttc tggagatgct attgtagccc cagatgcgcc tatccgtgag caattttata
     8821 aggaaattga ctttatgcac cgtaagtggc cggatgttca gaagtatctg gtttattttc
     8881 aaaattttac caacacccat gaaaaggtgg aagttattcg agagcgttat gaacaggcta
     8941 tcaatgagcc aggtgtggta ggtatcaata ttggaacgcg cccagactgt ttaccagacg
     9001 aaaccatcga atatttggct gagttatcgg agtgcatgca tgtgacggtt gaattgggct
     9061 tgcagaccac ttatgaagca acctctgacc tgattaaccg tgtgcattct tatgaattgt
     9121 aggtggaaac ggtcaagcgt ttgagaaagt atcccaagat tgagattgtt tcccatttga
     9181 tcaatggttt gcctggtgaa acccatgaga tgatggttga aaatgtccgc cgttgtgtca
     9241 cggataacga tattcaaggg attaaactgc atttgctcca tcttatgaca aatactcgta
     9301 tgcaacgaga ttaccatgag ggacgtttac aattgatgag tcaggacgaa tatgtcaggg
     9361 tcatctgtga ccaactggaa attattccca aacatatcgt catccatcga attacaggag
     9421 atgcgcctag ggatatgctg attggtccta tgtggagcct caaaaaatgg gaagtcctca
     9481 acagcattga gatggagatg cgacgtcgtg gaagtgttca aggatgcaag gctgtaaaac
     9541 aggagtttga aaatgaaaag accacttgag atggcacatg attttttggc tgaggtcgtg
     9601 acaaaagagg atgtcgtagt ggatgcgact atgggaaatg gtcatgacac gcttttttta
     9661 gccaagctag ccaagcaagt ctatgccttt gatattcaga agcaagcctt ggaaaagacc
     9721 caagagcgtt tgcatcaggc tggcttgaca aatgcccagt taatcttgca aggccatgag
     9781 acactggacc agtttgtgac aaaagctaag gcagggattt ttaatctggg ctatttgccg
     9841 tcagctgata agtctgtcat cacccgaccg cagacaacga ttgaggcatt agaaaagcta
     9901 tgtggcttac ttgtcaaagg tggacgaatt gctattatga tttactatgg tcatgaagga
     9961 ggcgacctcg agagagatgc tgtcttggat tttgtaatcc agttgaacca acaagagtac
    10021 acagctgcca tttaccgaac tttaaatcaa gtcaacaacc cgccgttttt agtgatgatt
    10081 gaaaaattag agagatatag acatggataa acaataccta cgtgaaaagc tggatgccat
    10141 gcgccagaat tttgttgaat caacccacca cgaacgagcg gtgggcgtgc tagaccaagc
    10201 gcatatgagc aaaaaaatgc ttaaaatcaa gaaaaaatta gttgctcttg aaatggaacg
    10261 gtgccagaga aaaattgagc acaaagactg ttccaagatt gaccaaaaaa tcaaagagca
    10321 gaaggagata tttgaatcct gttgtaaaaa agattaagga ggacgtgcgt ggaattactg
    10381 atttacctca tcctattttt actggtcttg attgtctcaa gtacaaccaa taagctcctg
    10441 ccctttttgc ctctcccttt ggtgcagatt cttttgggaa ttgtgattgg tctcttttta
    10501 cccaatactg actttcatct taatacggag ttgtttttgg ccctggttat cggacccttg
    10561 cttttccgag aggctgaaga agcagatgtt acggctattt taaaacactg gcgaatcatt
    10621 gtttatctca tatttccagt gatttttatc tcgaccctga gtttgggtgg cttggcccat
    10681 cttctttggt tcagccttcc cttggcagct tgcttggctg ttggggcagc ccttggtcct
    10741 acggacttgg tggcctttgc ctctctttcg gagcgtttta gctttcctaa gcgcgtgtcc
    10801 aatattctta agggcgaagg actcttgaat gatgcttctg gtttggtggc ttttcaggta
    10861 gctttgacag cttggacaac tggagctttt tctctggggc aagctagcag ttcgctcatc
    10921 ttttcaatcc taggcggttt tttaattgga tttttaacag ccatgaccaa ccgcttcctc
    10981 cataccttct tgctaagtgt gcgcgcaacg gatattgcca gtgaactttt attagaattg
    11041 agtttgcctc tagtgacctt ctttctggca gaagaagtcc atgtttcagg gattattgcc
    11101 gtcgtagttg ctggaatttt aaaggcaagt cgcttcaaga aaatcacgct cctcgaagcc
    11161 caagtggata cggtgaccga gacggtctgg catacagtga cctttatgct caacggttct
    11221 gtctttgtga ttttagggat ggagttggaa atgatagcag aacctatctt gaccaatcca
    11281 atctataatc ctctactttt attgctatct ctcatcgccc ttacctttgt cctctttgtc
    11341 attcgtttta ttatgatcta tggctattat gcctatagaa cccgacgcct caagaaaaag
    11401 ctaaataagt atatgaagga catgtttctc ttgacctttt caggtgttaa gggaacggtg
    11461 tcgattgcta cgattctctt gataccaagt aatctagaac aggagtatcc tctcttgctt
    11521 ttccttgttg caggtgtgac gcttgtcagc tttttaacag gtctcttggt cttgcctcat
    11581 ctttctgatg aagaggaaga aagcaaggat tatctcatgc atatcgccat tttgaatgaa
    11641 gtaacgctag agttggaaaa agagttggaa gacaccagaa ataaacttcc cctctatgcg
    11701 gctattgaca attatcatgg acgtattgaa aatctcattt taagccaaga aaaccaggat
    11761 gatcaagaag actgggttgc tttgaaactc ttaattctta gtattgaaag tgatggtttg
    11821 gaacaggcct atgaagaggg gaacattagc aatcgtgctt accgagttta ccaacgttat
    11881 ctgaaaaata tagaacaagg aatcaatcgt aaacttgcct caagactgac ctattatttt
    11941 cttgtttcct tgaggatttt acgttttctt cttcatgaag tttttactct tggaaagacc
    12001 ttccgtagct ggaaggacaa ggagcaaagc cgtctccgtg ctcttgatta tgaccaaatt
    12061 gcagagctct atcttgccaa tacagagatg attattgaaa gtttggaaaa cctgaaggga
    12121 gtctacagac gctctttgat tagttttatg caggagtctc gtcttcgaga aacagctatt
    12181 atcagcagtg gtgcctttgt cgaacgggtt atcaatcgtg tcaaacccaa caatatcgat
    12241 gaaatgctga gaggctatta tctggagcgc aagttgattt tcgaatacga agaaaaacga
    12301 ttgattacga ctaagtatgc caagaaatta cgacaaaatg taaataactt agagaactat
    12361 tccttgaagg aagctgccaa taccctgccg tatgatatgg tggaattggt aaggagaaat
    12421 tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgg attatatatg
    12481 tgactgactt cgtcagtttc atctacaacc tcaaagcagg gctttgagca acctgcggct
    12541 agcttcctag tttgctcttt gattttcatt gagtataaga ttgtaagtga aggagtgtga
    12601 catgaaaaaa tggtggaaag agctgataga caagccttta ttaaaagctt ttttgcatta
    12661 ttatcaagca tcagatagtg agttgaccag tgtcgcagta gcctactatt ggttgatttc
    12721 gattttcccc ctgcttttgg tggtggtcaa tatcctccct tattttcaga ttcctgtggg
    12781 agaatttctg ggctttatga aagacgtcct gcccccaagc ctctatgaag gtgtggaaaa
    12841 aatagccaga gaagtcttga cgcaaccttc aacaggttta ttgagttttt ctgttttatc
    12901 ggcgttatgg agtttttcaa aatctatgaa tttcttgcaa aaagctttta acaaggctta
    12961 tggcgtcgaa aagagtcgtg gacttatttc ccatcagatg ctgagtctcc tagtcagttt
    13021 tggcttgcag ctcctctttg cgtttgcctt gtttttgatc ttatttggtc agatgatttt
    13081 ggctttactt gctcattatt ggacaaaaga tggcattatc tatcaggctt tacaagggtt
    13141 ggcaggtcct ctgatttacg ccctcctctt tgctatcttg gtcatgcttt attattttct
    13201 tcccaatcta tctaacagga aaattagcta taccctacca ggcagtgcct ttgttctctt
    13261 agtgatttta ggtttactaa ctctgttctc tagttatctc aattactatg tccatcattt
    13321 ggtggatgtc cgaattttag gttccgttct tcttgttgtt atgatgtttt ggtttatctt
    13381 gattgctaaa attgttatcc taggcgcggt tatcaatgcc agtatgcaga gtttgaaaga
    13441 tccggtcttt aaaaaagact aactatttat ccattataaa agcgcatagt atcaagactt
    13501 tatagaacct tgatactatg cgcttttgcg cttttgtgct ttgacagttt tttgtatagt
    13561 ttttgtccaa gatttctaaa tcatctgatt aaattcctat tttcccctat cattgtccct
    13621 gttttttcga aaatggaagg agtataataa atctatcaat aaaatgatag gaggaacttt
    13681 atgggtcata ttttcttttt tctaagtgtc tttttggcag ggattctatc cttcttttct
    13741 ccttgtatct tacctttgtt accggtctat acaggagtgt tactagatga taaggatggt
    13801 gctcaggctt ctagcggcaa attttcaatc tcagttacta gtttattacg aactctggcc
    13861 tttatagcag gaatttcctt tatatttatt ttgttgggct atggagctgg ttttttaggc
    13921 gatttgcttt atgcttcttg gttccaatat cttactgggg caattattat ccttcttggt
    13981 ttgcaccaaa tggagattct acactttaag gggctttata aggaaaagag gctacaactg
    14041 caaggacagg ggcaaaatgg taagggctat agtcaggcat ttttattggg cttgaccttt
    14101 agttttgctt ggacgccttg cgtggggccg gttctggggt ctgttttggc cttggcggct
    14161 tcaggtggtt caggagcttg gcagggagct ggtctcatgt tggtgtatac gctgggcttg
    14221 gcgctaccat tcttgcttct agctctgacc tctagttatg ttttgaaaca tttccgaaaa
    14281 cttcatccct atctcggaat cctcaaaaaa gtgggtggtt ttctcattat tgtgatggga
    14341 ctcttggttc tgtttggaaa tgcttcaatt ttaagtcaat tatttgaata aaatggaaag
    14401 gaatatcaat atgaaaaaat ggcaaacatg tgttcttgga gcaggttcgc tcctttgttt
    14461 gacggcttgt tcaggcaagt ccgtgactag tgaacaccaa acgaaagatg aaatgaagac
    14521 ggagcagaca gctagtaaaa caagcgcagc taaagggaaa gaggtggctg attttgaatt
    14581 gatgggagta gatggcaaga cctaccgttt atctgattac aagggcaaga aagtctatct
    14641 caaattctgg gcttcttggt gttccatctg tctggctagt cttccagata cggatgagat
    14701 tgctaaagaa gctggtgatg actatgtggt cttgacagta gtgtcaccag gacataaggg
    14761 agagcaatct gaagcggact ttaagaattg gtataaggga ttggattata aaaatctccc
    14821 agtcctagtt gacccatcag gcaaactttt ggaaacttat ggtgtccgtt cttacccaac
    14881 ccaagccttt atagacaaag aaggcaagct ggtcaaaaca catccaggat tcatggaaaa
    14941 agatgcaatt ttgcaaactt tgaaggaatt agcttaggag gtgtcttatg aatgataagt
    15001 taaaaatctt cttgttgcta ggagtatttt ttctagccat aaccggtttc tatgttctat
    15061 tgatacgaaa tgcagggcag acagatgcct cgcaaattga aaaggcggca gttagccaag
    15121 gaggaaaaac agtgaaaaaa acagaaatta gtaaagacgc agacttgcac gaaatttatc
    15181 tagctggagg ttgtttctgg ggagtggagg aatatttctc acgcgttccc ggggtgacgg
    15241 atgccgtttc aggctatgca aatggtagag gagaaacaac caagtacgaa ttgattaacc
    15301 aaacaggtca tgcagaaacc gtccatgtca cctatgatgc caagcaaatt tctctcaagg
    15361 aaatcctgct tcactatttc cgcattatca atccaaccag caaaaataaa caaggaaatg
    15421 atgtggggac ccagtaccgt actggtgttt attacacaga tgacaaggat ttggaagtga
    15481 ttaaccaagt ctttgatgag gtggctaaga aatacgatca acctctagca gttgaaaagg
    15541 aaaacttgaa gaattttgtg gtggctgagg attaccatca agactatctc aagaaaaatc
    15601 caaatggcta ctgccatatc aatgttaatc aggcggccta tcctgtcatt gatgccagca
    15661 aatatccaaa accaagtgat gaggaattga aaaagaccct gtcacctgag gagtatgcag
    15721 ttacccagga aaatcaaaca gaacgagctt tctcaaaccg ttactgggat aaatttgaat
    15781 ccggtatcta tgtggatata gcaactgggg aacctctctt ttcatcaaaa gacaaatttg
    15841 agtctggttg tggctggcct agttttaccc aacccatcag tccagatgtt gtcacctaca
    15901 aggaagataa gtcctacaat atgacgcgta tggaagtgcg gagccgagta ggagattctc
    15961 accttgggca tgtctttacg gatggtccac aggacaaggg cggcttacgt tactgtatca
    16021 atagcctctc tatccgcttt attcccaaag accaaatgga agaaaaaggc tacgcttatt
    16081 tactagatta tgttgattaa tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    16141 taccgtatgt agggttactg actccgtcag ttttatctac aacctcaaag ctatactttg
    16201 agctgacttc gtcagtttta tctacaacct caaagcggta ctttgagaaa cctgtggcta
    16261 tcttcctagt ttactttttg atttttattg agtataagag gtctttccta agcagttaga
    16321 ggagaatttt gctatactga tactagtaag tggtaaagga gcagagcatg acctacacaa
    16381 tcttaatcgt agaagatgaa tatctggtaa gacaaggttt gactaaactg gtcaatgtag
    16441 cagcctacga tatggaaatc atcggtcagg ctgaaaatgg aaggcaggct tgggaattga
    16501 tccaaaagca ggttccagat atcattttaa ccgatatcaa catgcctcat ctaaatggca
    16561 tccagttggc cagtctggta cgagaaacct atcctcaggt tcatttggtc tttttaacag
    16621 gttacgatga ttttgattat gccttgtctg ctgtcaaact aggtgtggac gactacctgc
    16681 tcaaaccctt ttctcgtcag gatattgagg aaatgttggg gaaaatcaaa caaaaactag
    16741 acaaggaaga gaaagaagag cagttacaag atttattgac taataggttt gaaggaaaca
    16801 tggcccagaa aatccagtct catctggctg atagccaatt tagtttaaag tctttagcca
    16861 gtgacttagg ttttagtccg acctatctga gttccttgat taagaaagag ttgggcttgc
    16921 cttttcagga ttatctggtg agagaacgtg ttaaacaagc caagctcttg cttttaacta
    16981 cagatctgaa gatttatgag atcgcagaga aggttggttt tgaagatatg aactatttta
    17041 cccaacgttt taagcagatt gcaggtgtga cacctcgtca gtttaagaag ggagaagacc
    17101 gatgaagcgt tcttctcttt tagttagaat ggttatttcc atctttctgg tctttctcat
    17161 tctcctagct ctggttggaa ctttctacta tcaatcaagt tcttcagcca ttgaggccac
    17221 cattgagggc aacagccaaa cgaccatcag ccagactagc cactttgttc agtcttatat
    17281 caaaaaacta gaaaccacct cgactggttt gacccagcag acggatgttc tggcctatgc
    17341 tgagaatccc agtcaagaca aggtcgaggg aatccgagat ttgtttttga ccatcttgaa
    17401 gtcagataag gacttgaaaa ctgttgtgct ggtgaccaaa tctggtcagg tcatttctac
    17461 agatgacagt gtgcagatga aaacttcctc tgatatgatg gctgaggatt ggtaccaaaa
    17521 ggccattcat cagggagcta tgcctgtttt gactccagct cgtaaatcag atagtcagtg
    17581 ggtcatttct gtcactcaag aacttgttga tgcaaaggga gccaatcttg gtgtgcttcg
    17641 tttggatatt tcttatgaaa ctctggaagc ctatctcaat caactccagt tggggcagca
    17701 gggctttgcc ttcattatca atgaaaacca tgaatttgtc taccatcctc aacacacagt
    17761 ttatagttcg tctagcaaaa tggaggctat gaaaccctac atcgagacag gtcagggtta
    17821 tactcctggt cacaaatcct acgtcagtca agagaagatt gcaggaactg attggacggt
    17881 gcttggcgtg tcatcattgg aaaagttaga ccaggttcgg agtcagctct tgtggacctt
    17941 gcttggggcc agtgtcacat ctcttcttgt ctgtctctgc ttagtgtggt tcagtcttaa
    18001 acgctggatt gctcctttga aggatttgag agaaaccatg ttggaaattg cttctggtgc
    18061 tcaaaatctt cgtgccaagg aagttggtgc ctatgaactg agagaagtaa ctcgccaatt
    18121 taatgctatg ttggatcaga ttgatcagtt gatggtagct attcgtagcc aggaagaaac
    18181 gacccgtcag taccaacttc aagccctttc gagccagatt aatccacatt tcctctataa
    18241 cactttggac accatcatct ggatggctga atttcatgat agtcagcgag tggtgcaggt
    18301 gaccaagtcc ttggcaacct atttccgctt ggcgctcaat caaggcaagg acttgatttg
    18361 tctctctgac gaaatcaatc atgtccgcca gtatctcttt atccagaaac aacgctatgg
    18421 agataagctg gaatatgaaa ttaatgaaaa tgttgccttt gataatttag tcttacccaa
    18481 gctggtccta caaccccttg tagaaaatgc tctttaccat ggcattaagg aaaaggaagg
    18541 tcagggccat attaaacttt ctgtccagaa acaggattcg ggattggtca tccgtattga
    18601 ggatgatggc gttggcttcc aagatgctgg tgatagtagt caaagtcaac tcaaacgtgg
    18661 gggagttggt cttcaaaatg tcgatcaacg gctcaaactt cattttggag ccaattacca
    18721 gatgaagatt gattctagac cccaaaaagg gacgaaagtt gaaatatata taaatagaat
    18781 agaaactagc taactcccag tcaattctgg gagttttatg cgtaattggg caaattttct
    18841 aggttgtcta tcttgctaaa aacaagaaaa aacggtatga tagaagagtg acaacagagg
    18901 taacagagat gaacgagaca cagattcaaa gagaaacacg tcaggtagtg gaagatgttt
    18961 tagaaaagac caatttgaag cagggggccc tttttgtttt aggtctatct tctagtgagg
    19021 tgctaggtgg tcagattggg aaagaatcca gtcaagagat tggtgagctg atagtggaga
    19081 ctatcttggg tattctaggt agtagaggta tccatttagc agttcaaggt tgtgagcatg
    19141 tcaatcgggc tctcgttgtt gaacgtcagg tggcagagca gtttgatctg gaaattgtca
    19201 gtgtccatcc tactcttcat gcaggaggtt cggggcagtt ggcagccttt aagtttatgc
    19261 aggatccagt tgaggttgaa tttatcaagg ctcatgctgg attggatatc ggagacactg
    19321 caattggcat gcatgtcaag catgttcagg ttccgattcg ccctattttg agagagattg
    19381 gtcatgccca tgtaacggca ctggctagtc gtccaaaatt aatcggaggt gcgcgtgcgc
    19441 actatccgca agacgctatt agaaagtctt gagaatgaat aaaggagata aaagtgttta
    19501 aaaaagaccg tttttcaatt cgtaagatta agggagttgt aggctctgta tttcttggaa
    19561 gccttttgat ggctccttct gtagtggacg cagccaccta tcactatgta aataaagaga
    19621 ttatttcaca agaagctaaa gatttaattc agacaggaaa gcctgacagg aatgaagttg
    19681 tatatggttt ggtgtatcaa aaagatcagt tgcctcaaac agggacagaa gcatctgttt
    19741 tgacagcttt tggtttgctg actgttggga gcttgctttt aatctacaag agaaagaaaa
    19801 ttgctagcgt ctttctagtt ggagctatgg gattggtagt tcttcctagt gcagaggctg
    19861 tagacccagt tgcgacccta gcgctggcta gtcgagaggg tgttgttgaa atggatggct
    19921 atcgctatgt tggttatcta tcaggtgaca tcctcaaaac gcttggcttg gacactgttt
    19981 tagaagaaac ctcagcacaa cctggagagg tgactgtagt cgaagttgag actcctcaat
    20041 caacaacaaa tcaggagcaa gctaggacag aaaaccaagt agtagagaca gaggaagctc
    20101 caaaagaaga agcacctaaa acagaagaaa gtccaaagga agaaccaaaa tcggagataa
    20161 aacctactga cgacaccctt cctaaagtag aagaggggaa agaagattca gcagaaccag
    20221 ctccagttga agaagtaggt ggagaagttg agtcaaaacc agaggaaaaa gtagcagtta
    20281 agccagaaag tcaaccatca gacaaatcag ctgaggaatc aaaagttgaa ccaccagtag
    20341 aacaagcaaa agtcccagaa caacccgtgc aacctacaca agctgagcaa ccaagtacac
    20401 caaaagaatc atcacaagaa gacaattcta aagaagatag gggagcggaa gagacaccga
    20461 aacaagaaga tgaacagcca gcagaagccc cagaaatcaa ggttgaagaa ccagtagaat
    20521 caaaagagga gactgttaat caacctgttg aacaaccaaa agtggaaacg cctgctgtag
    20581 aaaaacaaac ggaaccaaca gaggaaccaa aagttgaagt aacaacagtt gaaacaagag
    20641 aagaagtcat tccttttgaa acaaaagaac aagaagatga cactttaaaa cgcggaacaa
    20701 gacaagtagt tcaaaaaggt gtagaaggta agaaacaaat tactgaaact tataaaacaa
    20761 ttcgtggaga aaaaacaaat gaagctccaa tagttaagga aacagtaatt gaacaacctc
    20821 aagatgagat tatcaaaaaa ggtacaaaag ggttagaaaa accaacatta caatgggcta
    20881 atactgaaaa agatgtcttg aagaagagtg ctacagctag ttatacattg actaaaccag
    20941 ctggtgtaga aattaaatca attaaattag cgctaaaaga taaagatgga caacttgtta
    21001 aagaagtaac ggtagctgaa aataatttaa atgctacttt agataaattg aaatattatc
    21061 aaggttacac cctatctact acaatggttt atgatcgtgg agaaggtgaa gaaactgaga
    21121 aattagaaga taaacaaatt caattagacc ttaaaaaagt tgaaatcaaa aacattaaag
    21181 aaacaagctt aatgaatgtt gatgctgagg gtaacgaaac tgataagagc ttattatctg
    21241 aaaaaccgac agatgtttcc caattgtatt tacgtgtgac aactcatgac aacaaagtga
    21301 caagacttgc ggtaagttca gttgaagaag ttgttgttga tggaaaaaca ttgtacaaag
    21361 ttgtagccaa agctccagac ttagttcaac gtagagctga tgatacactg agtgaagaat
    21421 atgttcatta ttttgaaaaa caattaccaa aagtaaataa tgtatactac aacttcaatg
    21481 aacttgtaaa agatatgcaa gctaatccaa cgggtgagtt taaacttggt gcagacttga
    21541 atgcagcaaa tgtaaaacct aacgggaaat cttatgtaac gaaaccattt aaagggaaat
    21601 tactaagtaa tgacggcggt agatttacaa tccataatat agaacgtcca ttattcgcta
    21661 atattgaagg tggtaaaatc cacgatatta atcttgctaa tgtaaatatt aatatgcctt
    21721 gggctgataa agttgctcct attgcaaatg taatcaaaaa taatgcaacc attgaaaatg
    21781 tcaaagttac tggtaatgtc ctgggtaaag actgggtatc tggatttata gacaaaattg
    21841 acggcagtgg taaattaatt aatgttgcat ttatcggtaa tgttacatct gttggtactg
    21901 gaggcaactt cttaacaggt attgtgggag aaaactggaa aggatacgtt gaaagagcat
    21961 atgttgatgc tcatattaaa ggtaagcgag ctaaagctgc gggtatagcc tactggtcac
    22021 aaaaccaagg gaataacttt actattggat ctgagggggc tattaaaaaa tctgttgtta
    22081 aaggtacaat agacgttgaa aaaccaattg aagttggtgg cgctgttgga agttttactt
    22141 atcatggttc tattgaagat actgtttcta tgatgaaagt taagaacggt gaaattttct
    22201 atggttctaa agatattgat gatgatccat attacacagg taaccatgtt aatcgaaact
    22261 acgttgttat aggtgttagc gaaggtactt ctacatatag atattctaat cagcataatc
    22321 gtattaaacc tataacacaa tcagaagctg atgttaaaat tgcagagaca gctatcacag
    22381 cagataaatt tacaattacc gatccaattg taaataaact taacgctctc acaacacgtg
    22441 ataatgagta tagaacaaca caagattacg aggcgactcg cgaacaagct tatcgcaata
    22501 ttgaaaaact tcaaccgttc tataacaaag aatggattgt caaccaaggt aacaaacttg
    22561 tagaaggttc aaacttgttg acaaaagaag tattatctgt tacaggtata aagagcggtc
    22621 aattcgtaac tgatttgtca gatattgata aaattatgat tcactattct gatggagcaa
    22681 aggaagaatt gaatgttact cgtcaagaat caaacgttca acaagtgcgt gagtacagta
    22741 ttacagattt agatatactc tatacaccaa atatggttga gaaagatcgc gctcagttga
    22801 tgactgatgt aaagtctaag ctatctagtg tagaattaga gtctgatgga gtgcgtcaac
    22861 ttctcgttaa gagagatact aagaaagatg cgaatgcaaa tagtgtaggt cgtcaaaatg
    22921 gatacattag agatttattc cttgaagaaa gtttctctga agttaaagca aatcttgaca
    22981 aattggtaaa acagatactt gaaaatgaag atcatcaact caacgataac gaattagctg
    23041 aacgtgcttt actgaagaaa gttgaagata ataaagctaa aatcatgatg ggactggcat
    23101 acttaaatca atattatgct tttaaatacg atgagttatc aattaaagat attatgatgt
    23161 ttaaacctga tttttatggt aagacagcaa gtgttattga tagattgatt aatattggtt
    23221 ctgcagaaaa taatctaaaa ggagatagaa cgcaagatgc ttatcgtggt ataatttcag
    23281 gggcaacagg taaaggtagt ttacatgact tcttgaccta caatatgaaa ttattcacaa
    23341 atgaaacaga cataaatgtt tggtttaaga aagctattga aaagaatgct tatgtagttg
    23401 aacaaccatc aacgaaccct gcatttgcca acaaaaaata tcgtctatac gaaggaatta
    23461 ataatgggca acatgggcgc atgattttac ctcttcttaa cttgaaaaat gcacatctat
    23521 ttatgatttc aacatacaac actatctcgt ttagttcgtt tgaaaaatat ggcaaagaca
    23581 cagatgaaaa acgtgaaaag ttcaagagtg agatcaataa acgtgctaaa gagcaggtta
    23641 attacttaga tttttggtca cgtcttgcta ctgataatgt ccgtgataaa cttcttaaga
    23701 gtcaaaatgt agttccaact cctgtatggg ataatcataa tagcccgaac ggatgggcta
    23761 gtagacatgg tcatatagat gggaaaccag attatgctcc aattcgtgag ttctttgggc
    23821 gaatcaataa gtatcatggt tacaaatatg gatatggcgc atatgcatat atttttgcag
    23881 cacctcaacc gatggatgca gtttatttcg ttatgacaga tttaattagt gactttggaa
    23941 catctgcatt tacacatgaa actactcacg ttaatgatcg tatggcttac tatggtggtc
    24001 attggcatcg tgaaggtacg gatcttgaag catttgctca ggggatgtta caaacgccat
    24061 cggtatcaaa tccaaacgga gaatatggcg cattaggtct aaatatggcg tatgaacgtc
    24121 aaaatgatgg aaatcaatgg tataacacaa atccaaacga ccttacaact cgtgcagaaa
    24181 ttgataacta catgaaaggt ttcaacgaca cattaatgct tcttgattac cttgaaggag
    24241 aagctgtcct caataaagca aatcaagatt taaacaacgc ttggttcaaa aaagtggata
    24301 agcaacttcg tggagctagt acgaagaacc agtatgataa agttcgtgat ttaacggcag
    24361 atgagaaaaa tatcactcta aattcagtag atgatttagt tgataataat tttatgacga
    24421 aacacggacc tggaaacaat gtatatgatc caactgggtt cggaacggcc tacgtgactg
    24481 tacctattac tgcaggtatt tatggaggta acacaagtga aggtgctcct ggttctatgt
    24541 cattcaaaca taacacattc agaatgtggg ggtactacgg atatgaaaaa ggattcttaa
    24601 actatgcttc taacatgctg aaaaatgagt ctagacaggc tggtcacaat actctaggtg
    24661 atgatttcat cattaagaaa gtttctgata ataaattcag tacattggaa gattggaaga
    24721 aagcatactt caaagaagtt gtagataaag ctaaagcagg atttaaccct gttacaatcg
    24781 acagtactac atatagtagt tacgatgact tgaagaacgc atttgctgca gcagttgaaa
    24841 aagataaagc tactcttaaa aatggatcag ttaaatctga caatactgtt gcacttaaag
    24901 agaaaatata caaaaaactt cttcaacaaa cagatagctt taaaacttct atctttagat
    24961 aatcgaaatc tctcatctgc tttgcggatg agggttttct tattttatgc tatacttaag
    25021 gtatgcatag aaaaacagtg attgatttta ggactttagg ggagagatat acctttaccc
    25081 agcctattaa agagttgaaa acgagaaatg tagaagaagt ggcagatttg ctggcacaag
    25141 tggaaagcta ccaagagcaa ggttattatg tggtggggta tgtcagctac gaggctgcac
    25201 ctgcttttga ggaaaaatta gcagttcaca aggatcccct actgggagag tacctgcttt
    25261 attttactgt tcatgatagc gtagaaactt cccctattcc tctgacttat gaggatattg
    25321 atttgccctc aaattggcag gaagtaacgt ctgcagcaga ctatgaaaag gccattgccc
    25381 agatacacca tcacttgagg cagggagaca cctatcaggt caactacacc gtccaactca
    25441 agcaaaagtt aagtgccaat ccttttgcca tctacaatcg tatggtggta gagcaggagg
    25501 cgggctacaa tgcctatgtg gaacatgacg agatggcagt gatttccatg agcccagagc
    25561 tcttttttga gcaaaatgat cgcgagttga caacacgacc aatgaagggg acgactcagc
    25621 gtggggtaac tgaccaagaa gatcttgaac aggccagttg gttggaacag gatcccaaaa
    25681 atcgctctga aaatatgatg attgtggacc tcttgcgcaa tgatatgaac cgtatttctg
    25741 aagttgggag cgagcacgtg gagcgtctgt gccaggtaga gcagtattca actgtttggc
    25801 agatgacttc gaccatcaag agtcagttgc gagaggatgt ggaccttgtt gaaatcttcc
    25861 gctcactctt tccttgtggt tccataacgg gtgcaccgaa aattgcgaca atggagatta
    25921 tcaagaactt ggagcctcaa ccgcgtggag tctactgtgg aacgattggt ctcttgcttc
    25981 caaatggacg acggattttt aatgtggcca ttcgtaccat tcaacttcac aaaggtcaag
    26041 ccatttatgg agttggcgga gggattactt gggatagcac atgggaatct gaataccgag
    26101 aggttcatca aaaggcagca gttctttatc gtaaacaagc tcgcttccaa ctgattacga
    26161 caggggaaat cagccagaag aacctgctgt ttgaagatca acatctggaa agactgagaa
    26221 aagctagtcg ttattttgcc tttccttttg atgcagaaga cttgggacac aagattgagg
    26281 aagagtgtca ggattgtgaa gctaatcaag attaccgctt gcgaatcagc cttagcaaat
    26341 ctggagagat agaagtcaat cgtcaagtat taacccctct cagtacaagc ttttgtcagg
    26401 cccaagtctg ccttcaggaa gctgctttga atcaatcctt tacctacttt aaaaccactc
    26461 accgaccgca tttgagccta ggagaacaag agaagattta ccacaataag tcaggagaac
    26521 tgcttgaaac ctctatagga aatttggttc tgaaaatcgc tggaaaactc tacacaccgc
    26581 ctatccgact tggaatcttg ccaggaattt accgtcagca tttgctagaa acaggacagg
    26641 tagaagagaa agtcttgacc ttggcagatt taacccaagc agaagctatt tacggctgta
    26701 atgcagtgag aggcttgtat gaactaagcc ttgaggagaa ctagatgaaa ttgatatttt
    26761 tgcatgggtt agggcagtca gcagagtctt ggaaagaagt tcggaatcta ctgacagatt
    26821 atccttctga ggctatcgag ttatttcctt ctggagttag taactatcaa caagctaaag
    26881 agcgagttta tcagcaccta gctcaagaga cagaaccttt tgttttgatt ggattgtcct
    26941 taggtgctgc cctgacacta gaactttcca gttacgattt accaaatctt cgagctttga
    27001 ttctgtcagg atgcccgctt aaattagcag ggaatatcct attttatctt cagttgctga
    27061 tttttaaact acttcccaaa agagtatttg aaaaacaggg agcagacaag actcttatgg
    27121 tcggagtttc tgaggaattg aagacacttg atttaacgga tatagcaggg acttgctcct
    27181 atccaacctt actaatttgt ggcagcaaag ataaaccgaa tcttagttct atgaaagctc
    27241 tccatagact gttaacagat tcgcagtttc agatcattcc tgatggccct catgtcttga
    27301 ataaggcaaa accaaaggag tttgtagaaa agaccagaag tttccttgaa ttgctgaaat
    27361 aagtttgata aaataatatt gaaatcgata tcataattat gggagaaaga aatgaataaa
    27421 cgtctatttt taaaaatgag tctggtgacg ttgccaattt tagccttgtt ttcacaacca
    27481 gttttggcgg aagaaaacat ccatttttcg agctgtaagg aagcttgggc gaatggctat
    27541 tcggatattc acgagggaga acctggttat tctgccaagt tagaccgtga tcatgatggt
    27601 gtggcttgcg aattgaaaaa tgctcctaag ggtgctttta aagcaaaaca gtcaacggct
    27661 attcaaatca acacaagttc agcaacaaca agtggttggg ttaagcagga cggcgcttgg
    27721 tactactttg atggaaatgg aaatctagtg aaaaatgcat ggcagggaag ctattacctg
    27781 aaagctgatg gtaaaatggc acagagtgaa tggatttatg actcttctta tcaagcttgg
    27841 tattatttga catcagatgg ttcttatgca aaaaatgcat ggcaaggagc ttattacctt
    27901 aaatcaaacg gtaaaatggc acaaggtgag tgggtttatg attcttctta ccaagcatgg
    27961 tattacttga aatcagatgg ttcatatgct cgcaatgcat ggcaaggaaa ctactatttg
    28021 aaatcagatg gtaaaatggc taaaggtgaa tgggtttatg atgccaccta tcaagcttgg
    28081 tattatttga catcagatgg ttcttatgct tacagtacat ggcaaggaaa ttactatcta
    28141 aaatcggatg gtaaaatggc tgtcaatgaa tgggttgatg gtggacgtta ttatgttggc
    28201 gctgacggag tttggaagga aggtcaagca agtacagttt cttctagtaa tgatagcaat
    28261 agtgaatatt ctgctgcttt aggaaaggca aaaagttata attcgttatt ccacatgtca
    28321 aaaaaacgta tgtatagaca attaacttct gattttgata aattttcaaa tgatgcagct
    28381 caatatgcca ttgatcattt agatgattaa cagatactgg taggcgaaaa aattccttat
    28441 aattcattta cgcaaacctt tgcataagtg aatggatttt gatatactag tactgtaagc
    28501 attttcaatt actacacaat gaaaaaggag aatatgatga gtcaaaagat tattgggatt
    28561 gaccttggtg gaacttctat caaatttgca atcttaacaa cagtaggaga aatccaagga
    28621 aaatggtcaa tcaagaccaa cattttggat gagggaagtc atatcgttga tgatatgatt
    28681 gagtctattc aacatcgttt ggacttgctt ggattggcag cagcggactt ccaaggcatt
    28741 ggaatgggat caccaggtgt ggttgaccgt gacaaaggga ctgttatcgg tgcctacaac
    28801 ttgaactgga aaacccttca accaatcaaa caaaagattg aaaaagcttt gggcattcca
    28861 tttttcatcg ataatgatgc caacgtagca gctcttggtg agcgctggat gggtgctgga
    28921 gataaccaac cagacgttgt ctttatgaca ctcggtactg gtgttggtgg tggtattgtc
    28981 gcagaaggca aattgcttca tggtgttgct ggtgcagcag gtgaacttgg tcatatcact
    29041 gttgactttg accaaccaat ctcatgtact tgtggtaaaa aaggctgcct tgagacagtt
    29101 gcttcagcaa cagggattgt caacttgact cgtcgctatg ccgatgaata cgaaggcgat
    29161 gcagccttga aacgcttgat tgataacgga gaagaagtaa ctgctaagac tgtctttgat
    29221 ctcgcaaaag aaggagacga ccttgctttg attgtttacc gtaacttctc acgttacttg
    29281 ggaatcgctt gtgctaacat tggttcaatc ctaaacccat caacaatcgt catcggtggt
    29341 ggtgtgtcag ctgcgggaga attccttcta caaggtgttc aaaaagttta cgatgaaaat
    29401 agtttcccac aagtacgcac atctactaaa ttagctcttg caactctagg aaatgacgct
    29461 ggagttatcg gagcagcatc acttgtattg caataagata atgagagagg aaaatgctaa
    29521 cctagagtta gtaatgttcc tcttttattt tttatgctat actagttagg acaatgaatg
    29581 aggtgaaacg aaatgacaaa agcagatacg atttttaaag agaatattga acgcattcta
    29641 aaggaaggtg ttttctcaga gcaggctcgt cctaagtaca aggatgggac tgttgctaat
    29701 tccaagtatg taactggtgc cttttccgag tacgacttat ctaaaggaga atttcctatc
    29761 acaaccttgc gtcccattgc aatcaaatcc gccatcaaag aagttctctg gatttaccaa
    29821 gaccagtcaa atagcttaga agtgcttaat gacaagtaca atgttcacta ctggaatgac
    29881 tgggaagttg gagacacggg aaccattggt gagcgctatg gtgccgttgt taagaaacac
    29941 gacattatca ataagcttct caaacagttg gaaaccaatc cttggaaccg ccgcaatatt
    30001 atttcgctct gggattacca agctttcgaa gaaacagatg ggctgctccc gtgcgccttt
    30061 cagaccatgt ttgatgtccg ccgtatagat ggagaaatct atctggatgc gaccttgacc
    30121 cagcgctcga atgatatgct ggtggcccac cacatcaacg ctatgcagta tgtggctttg
    30181 cagatgatga ttgccaaaca ttttggctgg aaggttggga agttcttcta cttcatcaac
    30241 aacctccata tctatgataa tcaatttgaa caagctcagg aattgctccg tcgggagccg
    30301 tcaaactgcc aaccacgctt ggttttaaat gttcctgatg ggactaattt ctttgatatc
    30361 aaagcagaag attttgagtt ggtggattat gaccctgtta agccacagtt gaagtttgac
    30421 ctagctattt aaaagaatag aaaaaagaag ttgagaataa tcccaacttc ttttgtttct
    30481 taacgtgata ctcggcgacg agctgctttt ttacggtttt cttcgatgaa agctgctttt
    30541 tgctcttctg gttcgattac tttcttttta aatgcgtata ctgcacctgc aacggcagcg
    30601 acagttcctg cgacacctgt tacaagacct ttagcgaatc ctttagccat gagtcttcct
    30661 cctttatatt ctcaatcagc cagcctcctc aagaggtcac atttttctga ctgacctttt
    30721 tgtgttataa taatagtaac gaaaaaatgg gaatttttca aggaaaaaag atgaaaacaa
    30781 aaataattgt gattgttgga ccgactgctg ttggaaagac ggctctagct attgaagttg
    30841 caaagcgttt taatggcgaa gtggttagtg gagatagcca acaagtctat cgaggacttg
    30901 atattgggac agctaaggct agtccagaag agcaggcagc tgttcttcat catttaatcg
    30961 atgttagaga gataaccgag tcttactcgg cttttgattt tgtttcagaa gctaagatga
    31021 ctattgagga tattcacagc cgtggcaagc tagccattat cgccggtggg actggacttt
    31081 atatccagag cttgctagaa ggttaccacc taggtgggga gactcctcat gaggagattt
    31141 tagcttatcg agctagtttg gagccgtatt cagatgagga attagcccat tttgtggagc
    31201 aagcaggcct tgagattccc cagtttaatc gtcgtcgtgc tatgcgtgcc ttagaaattg
    31261 cccattttgg tcaggatttg gaaaatcaag agaccttgta tgaaccgctg attatctgct
    31321 tggatgatga acgtagtcaa ctttatgagc gtatcaacca ccgagtggat ttgatgtttg
    31381 aggctgggct tttggatgag gccaagtggc tgtttgacca ttcccctaat gtacaggctg
    31441 ctaaaggaat tggctataag gaactctttc cttatttccg tggagagcag acctttgagg
    31501 aagctagtga gagtcttaaa caggcgaccc gtcgttttgc caagcgtcag ctgacctggt
    31561 tccgtaatcg catgcaggtc accttttatc agattggaga atctggtgta caagaccgca
    31621 ttttaagcca gatagaggag tttttagatg attgaaacgg agaaaaaaga ggagcgagtc
    31681 ctgctgattg gtgtggaatt gcagggtatg gacagttttg acctctccat ggaagaattg
    31741 gctagtttag cgaaaacggc aggggcagtc gttgtagata gctacagaca aaaacgtgaa
    31801 aaatatgatt ccaagacctt cgtcggctct ggtaagttgg aagagattgc gcttatggtg
    31861 gatgcagaag aaatcactac tgtcatcgtc aacaaccgtc tgaccccaag gcagaatgtc
    31921 aatctagagg aagttctcgg tgttaaggtc attgaccgta tgcagttgat tttggatatc
    31981 tttgccatgc gggctcgaag ccatgaaggg aagttccaag tccacctagc ccaactcaaa
    32041 taccttttgc ctcgcttggt tggtcagggg attatgctca gccgtcaggc agggggaatt
    32101 ggttcccgtg gtcctggtga aagccagctg gagctgaacc gtcgtagcgt tcgcaatcaa
    32161 atcacggata tcgagcgcca gctcaaggtg gttgagaaaa atcgtgcgac tgtcagagaa
    32221 aaacgtttgg agtctagcac ttttaagatt ggtttgattg gttataccaa tgctgggaaa
    32281 tcaactatca tgaacatctt gaccagtaag acccagtatg aagcagatga gctctttgcg
    32341 actctggatg cgacaaccaa gagtattcat ctgggaggca atctccaagt aactttgaca
    32401 gataccgttg gctttatcca agatttgccg acagagttgg tgtccagttt caagtcaacc
    32461 ttggaagaaa gcaagcatgt ggaccttctg gttcatgtta tcgatgctag caatccttac
    32521 cacgaggagc atgaaaaaac ggttctctcc atcatgaaag acctggacat ggaagatatt
    32581 cctcacttga cgctttataa taaagcggat ttggtggagg atttcacgcc tacccaaacg
    32641 ccatataccc tcatttctgc caagtctgag gacagtcgtg aaaacttgca agcattattg
    32701 ctagataaga ttaaggaaat ttttgaagca tttaccctgc gagtgccttt ttcaaagtcc
    32761 tacaagattc atgatttaga gagtgttgca attctggaag aacgtgatta tcaggaagac
    32821 agcgaagtga ttacaggcta catttccgaa aaaaataaat ggaggttaga agaattttat
    32881 gactgatatt aaagctttgg ctctaaagta tggaggttat acaagtctgg acaaggtcta
    32941 tctggttcag cttctagctg gtaaaacaga gcaggagcag ttaacactta ttacacctcc
    33001 gccaagtgtt gttaatgcct actttgcaga actctatcag aaaaagagtc ctcaggctgc
    33061 gacggattat tttgcagaac tcagtcagga actgaatctt tacaatactg agccaagttt
    33121 caccctagaa aataagcctt ttattcggct taatctgtct ggtaaatcct ttggtttttg
    33181 ttatgatagt gacggccttg gtcggatttt ctctgaaaat gaggagaaaa tctcggatga
    33241 cttgctcttt gaaattgcgc aaattttccc ccatcaatta gtctttgaag agtctggtaa
    33301 gatttacatg aagcctgtcg aggacgagga agttgtcagc ctagaaagtc tcacagcttt
    33361 gactgatttg gaaagattgg ctgatggtcg aaaacgtctc aaaggctaca gccaagagga
    33421 tttattgcaa gaagctgctg ctttttctgg caaacgctat ttccgatcgg aaaaccgcac
    33481 agccatgtta tatattgatt aattagaaag tatcgaatgg atattcaatt tttaggaacg
    33541 ggggctggtc agccctctaa agctcgcaac gtttcaagtc tcgccctgaa acttttggat
    33601 gagattaacg aagtttggct ctttgactgt ggagaaggta cgcaaaatcg cattctggaa
    33661 accacagttc gaccacgtaa gatcagcaaa atctttatta cccatctgca tggagaccac
    33721 atttttggtt tgccaggttt cctttctagc cgtgcctttc aggccaatga agagcagaca
    33781 gatttggaaa tctacggacc tcaaggaatc aagtcatttg tcttaaccag ccttcgtgtg
    33841 tcaggttctc gtctgcccta ccgcattcat ttccatgagt ttgaccaaga ttctctagga
    33901 aaaatccttg agaccgataa attcactgtg tatgcagagg agctggacca tactattttc
    33961 tgtgttggct atcgtgtcat gcaaaaggat ctagaaggga cgctggatgc tgaaaaactc
    34021 aaggctgctg gtgttccgtt cggcccgctt tttggtaaaa tcaaaaacgg ccaggatctt
    34081 gttttggaag acggaactga aatcaaggcg gctgactata tctcagcgcc acgtccaggt
    34141 aagattatca ctattttagg agacactaga aaaaccaatg ctagtgtgcg tctggctgtc
    34201 aatgcagatg tcctagttca tgagtccact tatggcaagg gtgatgaaaa aattgctcgt
    34261 aaccatggtc actcaactaa tatgcaagct gcacaagtag cggtagaagc aggtgccaaa
    34321 cgcctcctac tcaaccatat cagtgcccgt ttcctctcaa aagatattag caaactcaag
    34381 aaagacgctg ccacaatttt tgaaaatgtc catgtggtca aagacttgga agaagtggaa
    34441 atctagcagt cacagaaagg ataagtatgc ctactattct cattaccgga gctagcggtg
    34501 gtctagctca agaaatggtc aaactcctgc ccaatgacca actcatcttg cttggtagaa
    34561 ataaggaaaa attagcccaa ctctacggaa attattccca tgcagaattg attgaaattg
    34621 atattaccga cgattcagcc ctagaagctc tggtaactga tctttatctc cgctatggca
    34681 agattgatgt cttgattaac aacgctggtt acgggatttt tgagggattt gaccagattg
    34741 ctgataaaga cattcaccag atgtttgagg tcaatacctt tgccctgatg aatctgtctc
    34801 gtcaccttgc ggctcgtatg aaggaaagca gcaaagggca tatcatcaac atcgtcagca
    34861 tggcaggtct aatagctact ggcaagtcta gtctttactc agcgaccaag tttgcggcta
    34921 ttggtttttc aaatgctctg cgactcgaac ttatgcccta tggagtctat gtgacaacag
    34981 tcaatccagg tccaatccga acaggatttt ttgaccaagc tgacccagat ggaacttatc
    35041 ttaaatcggt tgaccgcttc ctcttagagg cagatgcagt ggctaaaaag attgtcaaga
    35101 ttataggaaa aaataaacga gaactcaatc tcccgatttt gttgaaccta gcccataagt
    35161 tttatactct ctttcccaag ctagctgata agttggcagg ggaaactttt aattataagt
    35221 aaaaagaacc aatgtgcagg ttgttgctag cctacatatt ggttcttatc atttctcaac
    35281 tatcaaactg aacttcttct agtagatagt cgataaagcg ttcgcccatc ttagataggc
    35341 tggttttctc atgctggata tagaccagct caatcgggtc atcaatatcc agtgggatag
    35401 aaacgatatt gtctccgttt aggttgctgt tcaaaatccc tgtcgcaatg gtataaccat
    35461 ccaaaccaat caagagatta aagagggtgg cacggtcact gaccacaatg gatttcttgt
    35521 ggtgttcttg agaaagaatc tcttctgaaa agtagaagga gttgtgcgtc ccttggtcat
    35581 agctgaggta agggaaattc tccaaatcag acagtttcac cttgtctttc tttgccagag
    35641 ggttggtctt gctgacaaag atatgcggtt gcgctgtgaa gagatggtgg gatagcaggt
    35701 gattgtcatc cagcatcttg gttaaaacat cacggttgta actgtttaag aagaggaccc
    35761 cgacctcact gcggaagttc ttgacgtcgt cgataatctc ccaagtccga gtttcacgaa
    35821 ggaagagttc gtatttctcc atatcgcttt tcttgagcaa agagacaaag gcattgacca
    35881 caaaggcata gtgttgagac gaaacgctaa agagttcgcg gtgggcgaca ggatttttat
    35941 agcgttcctc cagaagctga gtttgctcga caacctgacg ggcataagag agaaactcca
    36001 tcccatcacg ggtcaaggta atacccttgg gattgcggat aaagatttca atgcccattt
    36061 cattttccaa atctcgcact gcattggaga gactgggctg agtgataaag agttgcttgg
    36121 ctgcctcatt catagagcca gtttcgacga ttttgataat ataatgtaat tgttgaattc
    36181 tcatgttttt attgtaccac acttgcctta gaatcgccaa agaagatagg aaaataagga
    36241 gcattgtgtg agtctcttct tgaaaaaaac acaaaatttt gctagaatga gtcttatgaa
    36301 aacattctat gatgtgcagc aattcctcaa acgatttggt attattgttt acatgggaaa
    36361 acgcttatat gatattgaac tgatgaagtt ggaactatct cggatttacg atgcagggtt
    36421 gatggataaa ttagactatc tagaggcaga agcagttctt cgcagagagc acaaggtaga
    36481 attggattat attgagaaaa atggagaaaa gaactaatgg ttacttggat tttgtgggca
    36541 cttatactag caatgttggc gtggatgggc tttaactatc ttcgtattcg ccgtgcggct
    36601 aaaattgtgg acaatgagga gtttgaagcc ttgattcgta cgggtcaatt gattgatttg
    36661 cgcgacccag cagaattcca cagaaaacat atccttcgtg cacgcaatat tccttcaagt
    36721 cagttgaaaa ctagtcttgc agcccttcgt aaagataaac ctgtccttct ctacgagaac
    36781 caacgtgcgc aacgagttac aaatgcagct ctttacttga aaaaacaagg tttttctgag
    36841 atttatatcc tttcttatgg cttggattct tggaaaggga aagtgaagac tagctaacct
    36901 tatttccaaa ttagtcgaat gataggatga agctattgtt ttatagtata attaatttgt
    36961 ataaaattta aagagttctt agaacatgaa agaaaagaag aaaaatccac tttttgtggg
    37021 tatcttgtcg attattcttg gtttgttatt tccaattgta ggcctgattt tgggtatcat
    37081 aggattggtc ttggctattt cataccaaaa agagtcacaa ttagagtata aaatagaaaa
    37141 gattcttaat atcttaggaa ttgtgatttc tgtagttaac tggattgtag caatcgcctt
    37201 aatttttcgg taaataaaag aaaaaagctt tagttttaaa gctttttttg ttttatcgac
    37261 tcatctctaa atagttttta atgatttgta actctttttg gttcaaaggg cggtagttac
    37321 cttctgctaa atctgggttc aatgtgaagt ccccaaattg gattcttttg aggctagtca
    37381 ccttaacacc aaccgagagg aacattttct tgatttgatg aaattttcct tctgaaatgg
    37441 tgatagaggc ttgactgagg gagggacttg cagatagaat ctctagtttt gcgggtttac
    37501 agacagtacc atctaaaaag acaattccct tttgaaaggc ttggatatgg tcaggtgtta
    37561 gaagtccatt aacctcaact tgataagtct tatcgacatg atattgggga tggaggagtt
    37621 ggaagcccaa gggaccgtta tcggtcaaga gaaggagtcc tgttgtatct cggtccagtc
    37681 ggccgacggc atagagcttg tcagactgga tgtcagatgg aagcaagtcc atgacggtcg
    37741 gaagttcctt gtctttgttg gctgtaacga caccagcagg tttatgaagc ataagatagg
    37801 tgtgttcata gccttgaatg attcgatcct gaaaaaggag ttcttgtagt cctgtatcga
    37861 tattttgagc tagggagcgg gctgggcaac catcgactag aatttcccct ctgaggagtg
    37921 cttgcttcat ggcctttcgg ctaacttttt cttgggctaa taaattatct aaacgcatac
    37981 tgtgcctatc ttatcataag tcgcttgctt tgaaaaggct tgacgtgaaa agaaaagcgg
    38041 agtgaaaaca tttacacttg cttgagttat gttatttatt tgaaattatg gtataatcgt
    38101 tcagttagaa aataaatttt gaatattata gaggaaatca tgacaaaatt aagagaagat
    38161 atccgtaaca ttgcgattat cgcccacgtt gaccacggta aaacaaccct ggttgacgaa
    38221 ttattgaaac aatcagaaac gcttgatgca cgtactgaat tggcagagcg tgctatggac
    38281 tcaaacgata tcgaaaaaga gcgtgggatt accatccttg ctaaaaatac tgccgttgct
    38341 tacaacggaa ctcgtatcaa cattatggac acaccaggac acgcggactt tggtggagaa
    38401 gttgagcgta tcatgaaaat ggttgacggt gttgtcttgg tcgtagatgc ctatgaagga
    38461 accatgccac aaactcgttt cgtattgaaa aaagccttgg aacaagacct tgtcccaatc
    38521 gtggttgtta acaaaatcga taagccatca gctcgtccag cagaagtagt ggatgaagtc
    38581 ttggaacttt tcatcgagct tggtgcagat gacgaccagc ttgatttccc agtggtttat
    38641 gcttcagcga tcaacggaac ttcttcattg tcagatgatc cagctgacca agaagcgact
    38701 atggcaccaa tctttgacac gattatcgac catatcccag ctccagtaga taactcagat
    38761 gagcctttgc agttccaagt gtcacttttg gactacaatg acttcgttgg acgtatcggt
    38821 atcggtcgtg tcttccgtgg tacagttaag gttggggacc aagttaccct ttctaaactt
    38881 gacggtacaa ctaaaaactt ccgtgttaca aaactcttcg gtttctttgg tttggaacgt
    38941 cgtgaaatcc aagaagccaa agcgggtgac ttgattgccg tttcaggtat ggaagacatc
    39001 tttgtcggtg aaaccatcac tccgacagat gcagtagaag ctcttccaat cctacacatc
    39061 gatgagccaa ctcttcaaat gactttcttg gtcaacaact caccatttgc tggtaaagaa
    39121 ggtaaatggg taacttctcg taaggtggaa gaacgcttgc aggcagaatt gcaaacagac
    39181 gtttcccttc gtgttgaccc aactgattca ccagataaat ggactgtttc aggacgtgga
    39241 gaattgcact tgtcaatcct tatcgaaaca atgcgtcgtg agggctatga acttcaagta
    39301 tctcgtccag aagttatcgt aaaagaaatc gacggtgtta aatgtgaacc atttgaacgt
    39361 gtacaaatcg acactccaga agaataccaa gggtctgtta tccaaagcct ttctgaacgt
    39421 aaaggtgaaa tgttggatat gatttcaact ggtaatggtc aaactcgttt ggtcttcctt
    39481 gttccagcgc gtggtttgat tggatactca actgagttct tatcaatgac tcgtggttac
    39541 ggtatcatga accatacctt cgaccaatac ttgccattga ttccagggga aattggtgga
    39601 cgtcaccgtg gtgcccttgt ttctatcgat gctggtaagg ctacaactta ctcaatcatg
    39661 tctatcgaag aacgtggtac gatctttgtc aacccaggta ctgaggttta tgaaggaatg
    39721 atcatcggtg aaaactctcg tgaaaatgac ttgacagtta acatcacgaa ggcaaaacaa
    39781 atgaccaacg ttcgttcagc tactaaggac caaacagctg ttatcaagac gcctcgtatc
    39841 ttgacacttg aagagtctct tgagttcttg aacgacgatg agtacatgga agtaacgcct
    39901 gagtctatcc gtttgcgtaa acaaatcctt aacaaagcag agcgtgagaa agctaacaag
    39961 aagaaaaaat cagctgaata agagctagaa agagagaaag atggtctatt tagtcctagg
    40021 aattttactg ctcctactct atgtatttgc gacaccagaa agcattaaag ggactgtcaa
    40081 tatcgtcgct atggtatgta ttttagtggc actcttgatt ttattggttc tatcttttct
    40141 gaaaattttt caattaccaa cagaaatatt cctagcaata gccatgttga tcctagctta
    40201 ctttagtgtt agagacatca cactcatgcc agtcaaaaaa agtaaaagaa gataaaaaag
    40261 agagtttcgg ctctcttttt gctaggaatt ttacaatagt ttgaattgct ttttagttaa
    40321 caccatctct tttacttgta tagtatgaat gggaaaaact tgaataaaat tcgttaatgg
    40381 catctatttt atatttggtt agtactggtt gcatattatt ttgaaataaa actactatat
    40441 aatattattc acagaggtaa tatagtaaaa tatctaaaaa attcgatttt ttccttgaca
    40501 acaaataata aaaaagttat tcttatcaca taagaaagcg gtttcaataa aagagagagg
    40561 aggatttatt ttgttgcatt gttgctcaag gtgtgctttt tttgaaagaa aaaataaaat
    40621 gaaaacgaaa aaacatagat tacttgctct agctcttatt tcaaatttta cattattggg
    40681 agctgcatca gctgctgtac aatatccaga tggaggagta tggacatatg gagaaggttc
    40741 aggaggtggt taggcttttt caaattacta tcatggtaaa aaatatcatt attcatccat
    40801 cgtgagcaga tgggatggtc attctgataa aggagaagct cctgctggaa aaacctccta
    40861 tgcatggatt tggactaaat ggggagaaca agtagcattt tactgtgatt atgactaatc
    40921 gttcattgat ttaaatcgtt ccccttgtaa gaaattacag ggggaatttt gtttacaagg
    40981 agtctactat gaagaaacta ttcatattat tatcaacttt ttttctcagc ttcttccttg
    41041 cttggattat tgtcttacgt gcgccacaat atttatatgc aagctatgat tccgtttctt
    41101 tacttcgtgt caaaaaagat actcaggaac cgacgcgtga ggtatttgaa caggaattgg
    41161 agaattttgc aaactcagaa cagagtttaa tagctagaag aattgtagag ccgagtaagg
    41221 atgggacgac tcactttact tatgcaactt atggtcaggg aactttacca aaagaattcc
    41281 aagaagctag ccaagaaagt cgtgaacgta gtgatccgct aaatagttat ctccttttgt
    41341 caggctcctt gacgaaagaa aagcttgccg ataaattagg agatttgggt tataaagcaa
    41401 gtgctgaccg aaagataccg ccctattttc ttgcttttcg aatattacta aatcccctta
    41461 ttttaattag tttagcaata tttggcttat ctttctttgc tttagtgatt atcactcgga
    41521 ttaaggaaat gagagcagca ggtataaaac tcttttctgg tcagactctc ttatccatca
    41581 tggggcattc tttatctact gatatcaaat ggctccttct atcagccctc ctttccttcc
    41641 taggtggggg agtcgttctt tttagtcaag gtttgtttta tcctatcttg ttagccacct
    41701 atggttttgg gattagtttc tatctgttgt ttttattggc gatttcaatt ttactaatgc
    41761 ttctttatct aatgagtttg aattacaaag cattagttcc cgttattaag gggagattac
    41821 cccttaaacg cctgatgatt ttaaccctat tgtgtcagtt ggttgctgtc tttacagtag
    41881 gatacgctgt taagacaggt ttgacgtctt accaacgatt gaaagaactt gaaatttcaa
    41941 aacaagcatg gcaggataga gcagactatt atcaaatttc ttttggctta ggtgatagag
    42001 gaaaagatac agaaaatcag agcaagtggt atgcctttgc caaggaagca atcgaagaag
    42061 aacaagctct ttatgtaaag gataatctgc tccattttgc caatccacaa ggaaaaaatg
    42121 aacagggaga gacactggat acctatagtc cagatgctaa tacgctctat gttagtccca
    42181 gttatttgga caaggaaaag gtcgtggtag atgctgagac caaacagaag ttagcccatc
    42241 tccaaaaagg tgagtttatc ctcttgctcc cagaacattt gcgctctcga gaagcagaac
    42301 ttaagaaagt ttttgaagaa agattgagtt attatggaaa atctggtgag gaggcaagtg
    42361 ctcctttgga ttatgagatg aaagcgcacg ttagttatct ttcaatggga gaaaagcggt
    42421 ttgtttataa taacggtgag aatcccgtat ccactcagta tttgactgat ccgattttag
    42481 tggtattcac gccgacttct acaggtgata gttttatatc cctatctagt tggtctatca
    42541 atgctggaaa acaactcttt atcaaaggat atgagagtgg gctagaactt ttgaagaaag
    42601 ctggaattta tgagcaagta tcctatctta aagaaggaag aagtgtttat ctaactcgtt
    42661 ataatgaagt tcaaactgaa acagcaactt taatcttagg agctattgtg gggatagcta
    42721 gttccttgtt actattttat tctgtcaatc ttctatattt cgagcaattc cgccgagata
    42781 tcttgattaa acgaatttca ggtttacgat tttttgaaac acatgctcag tatatggtta
    42841 gtcaatttgc cagttttgta tttggtgcta gtctttttat tttaagcagt cgagacttgg
    42901 tgattggctt gctcacttta ttagtctttc tagctagtgc agttctgacg ctttaccgtc
    42961 aagcgcagaa agaatctcgt gtttctatga caattatgaa aggaaaatag gatgattgaa
    43021 ctaaagaata tatctaaaaa atttggaagc cgtcagctat tttcagatac gaatcttcat
    43081 tttgaaggtg ggaaaattta tgccttaatc ggtacaagtg gctgtggtaa gacaacactc
    43141 ttgaatatga ttggacgatt agagccatat gacaaagggc aaatcatcta tgatggcact
    43201 tctcttaagg acatcaagcc ttctgttttc tttagagatt acttaggata cttatttcaa
    43261 gattttggct taattgaaag ccaaaccgtc aaagagaatc tcaatctggg tttagttggt
    43321 aaaaagttga aggaaaaaga gaaaatctct ttgatgaaac aagctctaaa ccgtgtaaac
    43381 ctctcttatt tggatttgaa gcaacctata tttgagttat caggaggaga agcacaacgt
    43441 gttgcattag cgaagataat tttaaaggat ccacctttga tcctcgcaga tgaaccaacc
    43501 gcttccttag accccaaaaa ctctgaggaa ttactttcca tcctagaatc tttaaaaaat
    43561 ccgaatcgaa ctattattat tgcgacccac aatcctctta tttgggaaca agtggaccaa
    43621 gttattcgag ttaccgattt atcacataga tgatatggca agattcagct agaagaaaga
    43681 gtcacaaaca cactttgtgg cttttttatt tccataaaaa tggtaaaata gtaggagtag
    43741 aaatggagtt cgagacatga aagtaataga tcaatttaaa aataagaaag tccttgtttt
    43801 aggtttggcc aagtctggtg aatctgcagc tcgtttgttg gacaagctag gtgccattgt
    43861 gacagtaaat gatgggaagc ctttcgagga caatccagct gcccaaagtt tgctggaaga
    43921 agggatcaag gtcattacag gtggccatcc tttggaactc ttggatgaag agtttgctct
    43981 tatggtgaaa aatccaggta tcccctacaa caatcccatg attgaaaagg ctttggccaa
    44041 gggaattcca gtcttgactg aggtggaatt ggcttatttg atttcagaag caccgattat
    44101 tggtatcaca ggatcgaacg gtaagacaac cacaacgact atgattgggg aagttttgac
    44161 tgctgctggg caacatggtc ttttatcagg gaatatcggc tatccagcta gtcaggttgc
    44221 tcaaatagcg acagacaaga acacgcttgt tatggaactt tcttctttcc aactcatggg
    44281 tgttcaagaa ttccatcctg agattgcggt tattactaac cttatgccaa ctcatatcga
    44341 ttatcatgga ttatttgagg aatatgtagc agccaagtgg aatatccaga acaagatgac
    44401 agcagctgat ttccttgtct tgaactttaa tcaagacttg gcaaaagact tggcttccaa
    44461 gacagaagcc actgttgtac cattttcaac acttgaaaag gttgatggag cttatctgga
    44521 agatggtcaa ctctacttcc gtggtgaagt agtcatggca gcgaatgaaa tcggtgttcc
    44581 aggtagccac aatgtggaaa atgcccttgc gactattgct gtagccaagc ttcgtgatgt
    44641 ggacaatcaa accatcaagg aaactctttc agccttcggt ggtgtcaaac accgtctcca
    44701 gtttgtggat gacatcaagg gtgttaaatt ctataacgac agtaaatcaa ctaatatctt
    44761 ggctactcaa aaagccttat caggatttga caacagcaag gtcgtcttga ttgcaggtgg
    44821 tttggaccgt ggcaatgagt ttgacgaatt ggtgccagac attactggac tcaagaagat
    44881 ggtcatcctg ggtcaatctg cagaacgtgt caaacgggca gcagacaagg ctggtgtcgc
    44941 ttatgtggag gcgacagata ttgcagatgc gacccgtaag gcctatgaac ttgcgactca
    45001 aggagatgtg gttcttctta gtcctgccaa tgccagctgg gatatgtatg ctaactttga
    45061 agtacgtggc gacctcttta tcgacacagt agcggagtta aaagaataaa atatgaaaaa
    45121 aattgtcttt acaggtgggg ggacggttgg acacgttacc ctcaatcttt tgttaatgcc
    45181 caagttcatc gaagatggtt gggaagtcca ctatatcggg gacaagcgtg gtatcgaaca
    45241 ccaagaaatc cttaagtcag gtttggatgt caccttccat tctattgcga ctgggaaatt
    45301 gcgtcgctat ttctcttggc aaaatatgct ggacgtcttc aaagttggct ggggaatcgt
    45361 ccaatcgctc tttatcatgt tgcgacttcg tccacagacc cttttttcaa agggaggctt
    45421 tgtctcagta ccgcctgtta tcgctgcgcg tgtgtcagga gtgcctgtct ttattcacga
    45481 atctgacctg tctatgggct tggccaataa aatcgcctat aaatttgcga ctaagatgta
    45541 ttcaaccttt gaacaagctt cgagtttggc taaggtcgag catgtgggag cggtgaccaa
    45601 ggtttcggat caaaaaaatc cagaaccaga tgaattggtg gatattcaaa gccactttaa
    45661 tcataaattg ccgactgtat tgtttgttgg cggttctgca ggtgctcgtg tctttaacca
    45721 attggtgaca gaccataaga aagaactaac agagcgctac aatattatca atctaactgg
    45781 agattctagc ctgaatgagt tgagccaaaa tctttttcgt gttgactatg tgaccgatct
    45841 ctatcaaccc ttgatggaat tggctgatat tgttgtgaca cgtggtggtg ccaatacgat
    45901 ttttgagctc ttggcgatag caaaattgca tgtcattgtg ccacttggtc gtgaagctag
    45961 tcgtggtgac cagattgaaa atgcagctta ctttgttaag aaaggctatg cagaagacct
    46021 tcaagaaagc gatttgacct tggatagttt ggaagagaag ctttctcact tactaagtca
    46081 caaggaagat taccaagcta agatgaaagc ttctaaggaa ttgaaatctc tagcagattt
    46141 ttatcaattg ttgaaaaaag atttatcata aggaaagtaa atgtcaaaag ataagaaaaa
    46201 tgaggacaaa gaaatcctcg aagaattgaa agagttatca gaatggcaga aacgaaacca
    46261 agaatatcta aaaaagaagg ctgaagaaga agcggctcta gctgaggaga aggaaaagga
    46321 aagacaagct cggatgggag aagaatctga gaagtcagag gacaaacagg accaggagag
    46381 tgaaacagac caggaagatt cagaatcagc taaggaagag tctgaagaaa aagtagcatc
    46441 ctcagaggct gacaaagaga aagaagagaa agaagaatca gagtctaaag agaaggagga
    46501 acaggataaa aagcttgcta aaaaggctac aaaggaaaaa ccagccaaag caaagattcc
    46561 tggtatccat atcttgcgag ccttcacgat tttatttcca agtctgcttt tattgattgt
    46621 ctctgcctac ttgctcagtc cttatgcgac catgaaagat attcgtgttg agggaacggt
    46681 gcaaactaca gctgatgata ttcgacaggc ttcaggcatt caggattcgg attatacgat
    46741 taaccttctg ctagacaagg caaaatatga aaagcagatt aagtctaact attgggttga
    46801 atcagctcaa cttgtctatc aatttccaac taagttcact attaaggtca aggaatatga
    46861 tattgtggcc tactatattt ctggtgaaaa tcattatcct attctttcca gtggtcagct
    46921 tgagactagt tctgtgagtc tgaacagttt accagaaact tatttatcag ttctctttaa
    46981 tgatagtgaa caaatcaagg tttttgtctc agaacttgct caaattagcc cagaactcaa
    47041 ggcggctatc caaaaggtgg aattagcccc aagcaaggtg acatccgatt taattcgatt
    47101 gaccatgaat gattcggacg aagtcttggt tcctctatct gaaatgagta agaaactgcc
    47161 atattacagt aagattaagc cacaattgtc agaaccgagt gtggtcgaca tggaagctgg
    47221 aatttacagt tacactgtgg cggataaatt aattatggag gctgaggaaa aagccaaaca
    47281 agaggccaag gaagctgaga aaaaacaaga agaagaacag aaaaaacaag aggaagagag
    47341 caatcgaaat caaacaaatc agcgttcatc gcgtcgctag gtttaccttt tctcttatag
    47401 ttctttagtg accatgtttt tacgtttaat atttgacatt tgtttttctt tatgttacat
    47461 ctgcaatgta atcgattaca aaataatttt tgatgaagaa ggtaacacat atgtcagatg
    47521 aagtattctt atttgaagaa attgaagaaa tcgtagcccc tacagatggt gaatttttgg
    47581 gggaagtttt acttggaact ggggtagttc tcttaattgg agtagcctgt tgttaaaaag
    47641 atagggagtg ataatcatgc aagataactt tttatttgag gaaattgaag aaatttcagt
    47701 accagttagt gatttttcag ctggacttgc aacaggtatc ggatttggtt tagcaatcct
    47761 tgctcttgct ggttgttgaa gtttgttcat ttactaacat caagcttttt caatttcatt
    47821 ttagacagtc atttgaattt tccgtattag tcttgcagca agagattaat agaattagtc
    47881 attattttat tgattgcgga ctgagggact agagtatgtt ttacttagcc cctcttttat
    47941 ttattaaagg ttaggtttgt tatgagaatt gttgataaga ttaagatatt acctactcct
    48001 tatgagggac agtatcatct atatatacca tccagtaaga aacatgtatt agttgggaaa
    48061 caggaaaaaa atggttagag caactaatag gtcaagaatt taccatatcg gacttattag
    48121 tgttagtagg gaagaaatat tatttaagat
//
LOCUS       DAVKJU010000005        48051 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVKJU010000005 DAVKJU010000000
VERSION     DAVKJU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.005"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(441..2168)
                     /gene="ezrA"
                     /locus_tag="VW191_000217"
     CDS             complement(441..2168)
                     /gene="ezrA"
                     /locus_tag="VW191_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEU9623064.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKGIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2250..4196)
                     /gene="gyrB"
                     /locus_tag="VW191_000218"
     CDS             complement(2250..4196)
                     /gene="gyrB"
                     /locus_tag="VW191_000218"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU9623065.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4211..4774)
                     /locus_tag="VW191_000219"
     CDS             complement(4211..4774)
                     /locus_tag="VW191_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9623066.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4859..5413)
                     /locus_tag="VW191_000220"
     CDS             complement(4859..5413)
                     /locus_tag="VW191_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEU9623067.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5424..6647)
                     /locus_tag="VW191_000221"
     CDS             complement(5424..6647)
                     /locus_tag="VW191_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9623068.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPRLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6684..9134)
                     /locus_tag="VW191_000222"
     CDS             complement(6684..9134)
                     /locus_tag="VW191_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEU9623069.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNSRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQESLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9334..10263)
                     /locus_tag="VW191_000223"
     CDS             complement(9334..10263)
                     /locus_tag="VW191_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9623070.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQVFLLDEEVCKLHPKNRFLDTGFYDYDPDMVR"
     gene            10403..10690
                     /locus_tag="VW191_000224"
     CDS             10403..10690
                     /locus_tag="VW191_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEU9623071.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10724..12058)
                     /gene="ciaH"
                     /locus_tag="VW191_000225"
     CDS             complement(10724..12058)
                     /gene="ciaH"
                     /locus_tag="VW191_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEU9623072.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12048..12722)
                     /gene="ciaR"
                     /locus_tag="VW191_000226"
     CDS             complement(12048..12722)
                     /gene="ciaR"
                     /locus_tag="VW191_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEU9623073.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12831..15377)
                     /locus_tag="VW191_000227"
     CDS             complement(12831..15377)
                     /locus_tag="VW191_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU9623074.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENNVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15500..15940)
                     /locus_tag="VW191_000228"
     CDS             complement(15500..15940)
                     /locus_tag="VW191_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEU9623075.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15915..16784)
                     /locus_tag="VW191_000229"
     CDS             complement(15915..16784)
                     /locus_tag="VW191_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEU9623076.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16762..17308)
                     /locus_tag="VW191_000230"
                     /pseudo
     CDS             complement(16762..17308)
                     /locus_tag="VW191_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            complement(17319..18017)
                     /locus_tag="VW191_000231"
     CDS             complement(17319..18017)
                     /locus_tag="VW191_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEU9623077.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPSGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18010..18276)
                     /locus_tag="VW191_000232"
     CDS             complement(18010..18276)
                     /locus_tag="VW191_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEU9623078.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18342..19268)
                     /locus_tag="VW191_000233"
     CDS             complement(18342..19268)
                     /locus_tag="VW191_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEU9623079.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19343..20158)
                     /locus_tag="VW191_000234"
     CDS             complement(19343..20158)
                     /locus_tag="VW191_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623080.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20162..20482)
                     /locus_tag="VW191_000235"
     CDS             complement(20162..20482)
                     /locus_tag="VW191_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9623081.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20689..20769)
                     /locus_tag="VW191_000236"
                     /pseudo
     CDS             complement(20689..20769)
                     /locus_tag="VW191_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20813..22810)
                     /gene="metG"
                     /locus_tag="VW191_000237"
     CDS             complement(20813..22810)
                     /gene="metG"
                     /locus_tag="VW191_000237"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU9623082.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIIAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22934..24193)
                     /locus_tag="VW191_000238"
     CDS             complement(22934..24193)
                     /locus_tag="VW191_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623083.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSSFTVSGKEEEIPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24195..24896)
                     /locus_tag="VW191_000239"
     CDS             complement(24195..24896)
                     /locus_tag="VW191_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623084.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24880..26079)
                     /locus_tag="VW191_000240"
     CDS             complement(24880..26079)
                     /locus_tag="VW191_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU9623085.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVARADCHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26365..27711
                     /gene="gor"
                     /locus_tag="VW191_000241"
     CDS             26365..27711
                     /gene="gor"
                     /locus_tag="VW191_000241"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU9623086.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            27908..28444
                     /locus_tag="VW191_000242"
     CDS             27908..28444
                     /locus_tag="VW191_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU9623087.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            28495..28581
                     /locus_tag="VW191_000243"
                     /pseudo
     CDS             28495..28581
                     /locus_tag="VW191_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(28650..28865)
                     /locus_tag="VW191_000244"
     CDS             complement(28650..28865)
                     /locus_tag="VW191_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEU9623088.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28963..29283
                     /locus_tag="VW191_000245"
     CDS             28963..29283
                     /locus_tag="VW191_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623089.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29313..29651)
                     /locus_tag="VW191_000246"
     CDS             complement(29313..29651)
                     /locus_tag="VW191_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623090.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29663..30382)
                     /gene="trmD"
                     /locus_tag="VW191_000247"
     CDS             complement(29663..30382)
                     /gene="trmD"
                     /locus_tag="VW191_000247"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU9623091.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30372..30890)
                     /gene="rimM"
                     /locus_tag="VW191_000248"
     CDS             complement(30372..30890)
                     /gene="rimM"
                     /locus_tag="VW191_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU9623092.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30921..31709)
                     /locus_tag="VW191_000249"
     CDS             complement(30921..31709)
                     /locus_tag="VW191_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9623093.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADVVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(31813..32056)
                     /locus_tag="VW191_000250"
                     /pseudo
     CDS             complement(31813..32056)
                     /locus_tag="VW191_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779614.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
     gene            complement(32286..32525)
                     /locus_tag="VW191_000251"
     CDS             complement(32286..32525)
                     /locus_tag="VW191_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEU9623094.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32545..32817)
                     /gene="rpsP"
                     /locus_tag="VW191_000252"
     CDS             complement(32545..32817)
                     /gene="rpsP"
                     /locus_tag="VW191_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU9623095.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32987..33196
                     /locus_tag="VW191_000253"
     CDS             32987..33196
                     /locus_tag="VW191_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623096.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33672..33884
                     /locus_tag="VW191_000254"
     CDS             33672..33884
                     /locus_tag="VW191_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623097.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            33967..34770
                     /locus_tag="VW191_000255"
     CDS             33967..34770
                     /locus_tag="VW191_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU9623098.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34827..36368)
                     /locus_tag="VW191_000256"
     CDS             complement(34827..36368)
                     /locus_tag="VW191_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9623099.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHELDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36370..36852)
                     /locus_tag="VW191_000257"
     CDS             complement(36370..36852)
                     /locus_tag="VW191_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9623100.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(36852..37937)
                     /gene="rlmN"
                     /locus_tag="VW191_000258"
     CDS             complement(36852..37937)
                     /gene="rlmN"
                     /locus_tag="VW191_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU9623101.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(37958..38488)
                     /locus_tag="VW191_000259"
     CDS             complement(37958..38488)
                     /locus_tag="VW191_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEU9623102.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(38644..39249)
                     /locus_tag="VW191_000260"
     CDS             complement(38644..39249)
                     /locus_tag="VW191_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU9623103.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39422..40459)
                     /locus_tag="VW191_000261"
     CDS             complement(39422..40459)
                     /locus_tag="VW191_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9623104.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40493..41428)
                     /locus_tag="VW191_000262"
     CDS             complement(40493..41428)
                     /locus_tag="VW191_000262"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEU9623105.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41981..43171)
                     /locus_tag="VW191_000263"
     CDS             complement(41981..43171)
                     /locus_tag="VW191_000263"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU9623106.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43386..44729)
                     /locus_tag="VW191_000264"
     CDS             complement(43386..44729)
                     /locus_tag="VW191_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9623107.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44869..45684)
                     /locus_tag="VW191_000265"
     CDS             complement(44869..45684)
                     /locus_tag="VW191_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9623108.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEVVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            complement(45736..47916)
                     /locus_tag="VW191_000266"
     CDS             complement(45736..47916)
                     /locus_tag="VW191_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9623109.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT        14258 a        10504 c         8684 g        14605 t
ORIGIN      
        1 cgtaaacaaa gaggttttag aggcctattt accgtgggac taaagttgta caagaaaagt
       61 gcaaataaga aatctccaga ttaggaacta tccgtgagtt ctctagtctg gagatttttc
      121 aatagacttc gttattggac ggttaccaag ttactcaaag tacagatttg aagctgcaga
      181 taagactgac gaaatcactt taatactaaa tgaaaatcaa aaagcaaact aagaagctag
      241 cctcaggctg tacttgagta cggtaaggcg acgctgacat gatttgaaga gattttcgaa
      301 gagtataaaa cactgttttg aggtggtgga taaatcggct gggtaaaaac tcaatctcag
      361 gagaaaatga agtaaatctc cccacaacta tagaaaaaga tttgattctg ctcctcacac
      421 aataaaatct ttttctttta ttaaaaacga atcgtttcac gtgttttctc atatgaggta
      481 acaaagcgat tggttacacc aggctctgcc acttccaatg cttgagaaat cttgtcaaat
      541 gaagcgtgat aatcaaattc tttttcaaaa atatctaaag cttcgttaaa tgcttcttga
      601 atgcgttcat caaatgagcg atagcggtta gaatattgca agagttgctc tgtcaaagtt
      661 gcatattgta caatattata agtttccgtt tctaaagctt ccatatcatt cgttgcaatt
      721 tcaagaactc gggtaacaga ttcaatgtta atcatttttt gttctaactc aaccattaaa
      781 tcctcggtat tattgcttgc cgtaaagaat aacttcaaga aagtttgtgg aatacctggc
      841 agattgcgtt tttccatgta tcgcttgata gtatggagac gattgacata aacattggcc
      901 ttttgacgtg cattaatatc atctttctca atttgtgtca ggcgctcact aactgaaatt
      961 tgctcatctt caataccttt tagttgagtt tgtaaatcct caagattttc ttcaagaact
     1021 gaataagctt gggttggttc ttcttgattt gaagttacct caacaatagc tgcctcaaaa
     1081 ctctctaatt ctgtctgaat acgacgaaca tggcttgcag ctgtctcagg aagtaaatag
     1141 gtcttgttca aacgtgcaat atcttctccc aataaagtat tattctcttt catatgttga
     1201 aggtaagttg gaagagttgc aagtagattt tccactactt tctgagcagc aatttctcga
     1261 gtaaaaatat catacaaggc attgatttcc tcttgtgcct gtccattctc atattcggca
     1321 ttatccaatt ccaactgacg aatattctct tggtttttct tgaatgcttc ataaagcaag
     1381 tggaaacgcg cttcaatatc cgtttcaaca aaatgataat tagcatcaat tagtttacga
     1441 taaccggctt ccaaatcctg taattgatct ggcaattctg tagaaagcgt cgtaaccaag
     1501 gctggaacac gatccacaat atgacttaag gccaaaatgt gattttctgt attatccaaa
     1561 atcactgcgg cttccacagg gtcacccgat gaattcaagg ttacaaattg tgaaaattca
     1621 gattggatat tttctaattg tttttcaatt tcatccaagg cttgaccata ctgttctgaa
     1681 ttttcagcaa ctctatgctg aagttcctca aataaatcca aagcatgaag aacacgacca
     1741 ctatttttag attcttgctt ctctaagtct gccaaagcat tgcgaattgc cgcaatatct
     1801 tcttcaatca aagtaatttg actctcaatt tggtcaattt gatgactggc cttgagaaaa
     1861 cgaaatgaat ggttatagcc ttctgcttca aagagattat tttcaatatc ggcaaaagag
     1921 ttgagagata aatcgaccca tttttgattc cattcacgga aagccacttg actttgtcca
     1981 atcaagtgca tattttttac agcttctact tcatcattta ctggaagatt gtatagttct
     2041 tcttttcttt cttctagcgc ctctaatctc ccctcgtttc gcttacgtag aaagattgcc
     2101 actacataag ccagaactaa aatgactgca attgcaacca ttaaataaat tagttgtcca
     2161 ttagacatat caaactcctt ttttacttga aacaatcgta acgattatat catatttttt
     2221 agaccaaggg aactacttct ccctattttt tagacatcaa gtgtactata gacagcattt
     2281 tcttcgataa actcacgacg aggctctact cgatccccca tcaacatatc aaagatttta
     2341 tctgcttctg cagcatcatc tacagaaact ctagccatca agcgatgttc gggatccatg
     2401 gttgtttccc acagctgatg atcgtccatt tcacctagcc ccttataacg ctgaatagtc
     2461 ggtttggtac gaccttcact ataacgggct aaagcttctt ggagtttgat ttcttgatct
     2521 gcacccggct ggatatattc tttaatctcg cttccaacct tgacaccata gattggtggt
     2581 tgggcaatat aaacataacc agcttctagg attggtttca tataacgata aatcaaggtt
     2641 aaaagaaggg tacgaatatg ggctccatcg acatcggcat cggtcatcaa aacgagtttt
     2701 tggtaacggg ctttcgaaac atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa
     2761 agactacgaa tttcttcgtt agctagaatc ttatccatac ttgctttttc aacgttcaaa
     2821 atcttaccgc gaattggaag gatagcctga aactcacggt tacgaccaga tttggctgat
     2881 ccaccagctg agtctccttc gacgatgaag agttctgttt cagcagggtt attagaagaa
     2941 cagtctgcta gtttccctgg aaggttggaa atttccaaac cagatttttt acgtgtgact
     3001 tcacgcgcac gcttggcagc cacacgagcc ttggcagcta aaatcccttt ttccacgata
     3061 cgtttggcaa tctgtggatt ttccatgagg aaatcggaga aggcttcact gaagaggcga
     3121 ttggtaatct tgaccacttc gctatttccc aatttggtct tggtttgtcc ttcaaactgt
     3181 ggatttgggt gtttaactga gataactgca gttaagcctt cgcgaacatc ttcccctgtt
     3241 aaattatctt cattgtcttt cagtaactta ttcttacgag cataatcatt gataacacga
     3301 gtcagtgccg tacggaaacc ttgttcatgc gttccacctt catgggtatg aatattattg
     3361 gcgaaactca tgacattttc atggtaacct gttgtgtact gcatggctac ctcaactgtg
     3421 atatcatcca tctcaccgtc tgtatagatt ggtgtatcaa agattacatc cttgttctcg
     3481 ttgatatatt caacgtaact agcaatccca ccttcataat gataatgctt ggtttgttcc
     3541 aaaccttggc gcttatcagt gatagaaatt tgaagaccgc gatttagaaa ggccaactct
     3601 tgaatccgtt tatttaattt atcaaaatca aaggttgttg tttcagtaaa gatttttggg
     3661 tccggtatga agtgaaccgt tgttccagtt ctatctgtat ccccaaccac ctcaaggtct
     3721 gcaacaacat gaccacgacg gtattcttgg taatgaatct taccattttt gtgaacatga
     3781 acgtctaatt gagtggaaag ggcattaact actgacgacc ccaccccgtg aagaccacct
     3841 gaaaccttgt atccaccacc gccgaacttt cctccagcgt gaaggactgt aaagacggtc
     3901 tcaacagcag gacggcctgt tttttcctga atatcgactg ggataccacg cccatcatcc
     3961 acaacagtaa tcgaatcatc tggctcaata aaaacttgaa tatggctggc aaatcctgcc
     4021 aaggcctcgt caattgagtt atcaacaatt tcccagacta gatggtgaag accttctttt
     4081 gaggttgatc caatgtacat ccctggacgc atacgaacag cctctaagcc ctctaaaact
     4141 tgaatttgac tggcatcata atcctgtgcc tgcagatttt tgatttcttc tgtcatctta
     4201 ttcctttttc ttatatgtct aatacttgtc ttaaattcac gatactggta aacaagtggg
     4261 tatcaagtcc tgcagcttga cctgcttcga tatcaatcgg ccgatcacca atgacaagac
     4321 cagagctaat ctgatacttt tctcttaaat aaagcatgga ttcgggattt ggctttctct
     4381 taaagcctga gctagaagtc accacttctg taaaataagc tgctatagag gttttttcta
     4441 aaatttccaa aacctgatca tttcgatgag agaccaaaaa atgacggcca ccttgatttg
     4501 aaatgacttc caataggtca gaaactcctt caaataaaat cgggtgttca agctctctgg
     4561 cttcattttc cttgtacttt tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg
     4621 caaaaggagt agaaaccttt aaagcttgat agacactgtc atggtcttgt gtgataccat
     4681 acagtgccaa tgtttcaaca aatgcagctg ttgaagtttc ataattatcc agtaaagttc
     4741 cacctaaatc ccagatgtaa tcgtgatatt tcataccctt cattatagca tttttttatg
     4801 aaaaaagcga tgatttccct gagttttcca cataaaaaac gagagaataa ctcccgtttt
     4861 actgattttt accaaagaca tctcgataga gcattcctgt tcgtaaactc tctgcgtccc
     4921 ctcctagttg ctccaccaac tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg
     4981 ttgtcaactg accatctacc actactgttt ccttgacgta gtgaccatca aggatttgct
     5041 cttgaacgcc gtcataacaa gtgtatcgct tatttttcaa tatctctgct tgattgaggg
     5101 caattggtgc cgcacaaatg gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt
     5161 cttgaatcaa ggtctgatta tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa
     5221 caatcatatc atagtctgat aaatttccat caaagacatg atctgctctt acttggattg
     5281 catgcgaacc cgttacttgc tcttcaaaac caaccatatc acatgtgata ttggctcgac
     5341 gcaagacatc tacaactgtc aaggcttcaa tttcttcaaa gccctgagct aacataactg
     5401 ctacttttac catgattttc tccttattta atttgtttta atacaacctt tttccgtttg
     5461 aatgggactt ttccgctctt ttcttcagct agattagtct ggtaactcat cgataaaagc
     5521 aaaccaacac caatcagatt actgataata gctgatcccc cttgcgaaat gaaaggcaag
     5581 ggaatccccg tcaaaggaag tagtccagtc acagcaccga tattctcaaa gatgtggaag
     5641 agcaacatca taatcaaacc tgtggaaata taagtgtaga actggttatt tgatttaaga
     5701 gtaatcttca acatacggta aatcaacatg agatagaggg caataaccag gacagagccg
     5761 ataaagccaa aatcttctgc aataaccgta aaaatcatat ctgactctcg aactgggata
     5821 agcagattcg aagcattaaa tccctgacca aataagccac cactcccaat ggcaatctgc
     5881 ccttgagcct gctggtaagt cgttgtttgg gcaaactcaa agggattgag ccaagccaaa
     5941 atccgattga tttggtaggt cggcattcca atctggtgaa gaaaagctcg tccgtccttg
     6001 ctaataaaga tagctaagaa accagcaact cctgttacag cagtcacaaa tactgggata
     6061 ataattttcc aagaaacccc tgataataaa acgattcctg agaaaatggc tacaaaaacc
     6121 aaagccgtcc ccaagtcact ttgaagtgct aaaagaacta ggactggaat ggtaaagaga
     6181 atcatccaga aaattaacaa aaagtccagc ggaaccgtgc gtctccattc cttatgtttc
     6241 tttgtaaatt ggacaatgac acgagccaac atgaggatat aagatatctt cataaattct
     6301 gacggttgga atagggtaat tccatttatt gatacccagt ttttggcacc cgttgatgca
     6361 actaatcttg gattataaaa tacaatcggc aagatcataa gtcccaagcc taaaatatat
     6421 agaaaggggg tcaccttcca aagaaattct gtattaaaga gcatgaccac aaaaccaatc
     6481 acaagcccca aggcaatcca ggcgacctgc tgccctaaaa tgggcagaat attattggga
     6541 taatcatgac taacggctat atagatagcc accacaccga tgaccagtag aaaaaatact
     6601 ggcaagagca aactataatc gactctagag tcgagagaac gtttcatata tactaacctt
     6661 atactttcat acaatactat ttatcaaagt tcattaaaaa atctatcaat agcctcgtca
     6721 acttcggatc gagagatggt tttaacagtt gcttcttctg ctagagatgc tactatttgt
     6781 ttaccgtatc gttttccgac gattctccta tccaaaataa gagttaagga acgttggtat
     6841 tcacgtctca tacttcttcc caaagcctgt tttaaacgaa taatggccat tggcaattga
     6901 taatcataaa aggcattttt cccttcttga ttcagttctt gattaatctt tttcgtcaag
     6961 gattcttgag gattttggaa aggaagtctc ggtacaactt gaatcacaaa aggatggctt
     7021 gaaaaatcaa ctccctccca gaaacttgct gcaccaagca agatttgttg ttcacctttt
     7081 tcaaagcgtt tctttagctg atgaacatcc ccatttttat actgggccaa gtggctaact
     7141 gtaagtaaat ccgatactgc tagaagcatg tctttagcgg taaagagaac caaaatcggt
     7201 tgctggaaag cttgaatttc cactagtaaa gcagctacct ctctggcata gacttctaag
     7261 gaggtttctg ttaccagagg gaaatctttg accataacca cttcttgttc ctgttttccc
     7321 cgagattcaa tcttgacaaa tttggcttca ggatagccta aaaggtctgc caaagaaacc
     7381 ctctgactaa tctcaagagt agccgatact cccaggactt ggcaactctc aggaagtaaa
     7441 gaggacagaa gagtacggcc tgattttgta gaagaaatct gaattttctt ttgactcgtt
     7501 tcagttactt caagccagta atcaccttca gctgtaaaat agcgaaccag ctcatcaaag
     7561 ccttccactt ctaattctga aaaatactga tggagattgt ccagagaatc taagatattt
     7621 ttcctagatt tgccagactg aaattgttct atcaagtaga gacactcaaa gcgaatactt
     7681 tctagtatcc gttgttgaac cctgttttct tctcctacta aagccttatc aattaaatcg
     7741 ataatagatt gtatatcgta ggtctcttga agcagatttt ctagagctaa caaaatcttt
     7801 tggacttcat caataatcag taaacggtca ctgacaaatt caggattatc ttcaagtctg
     7861 gtcacgagat aggcatgatt agtcactaaa agcttgcaag tctctgccct ttcttgacta
     7921 cgtttccaaa aatcttccgt cacaaataag ctctgggatg ataaattccc atcatgacga
     7981 aggtctgcta gaaaatgttg gtaacggtag agttgcccga tttcatccaa atctcctgtc
     8041 tctgtctcag taagccagac caagacttgc attttaaagc gtctaaataa gcgattttca
     8101 tcattttcct gcaaggaatg ataaaaggca tccaacttca gataattttg tggtcccttt
     8161 aagctatgaa tatctgtatg gaacacttcc ttgagacgtt taccttcttc ttccatgatt
     8221 tgattttgaa gaatctttgt cggaacacta agaacaattt gtcgctcttt ggattgagat
     8281 aaagcgggta agagatagcc ataggttttc ccaatccctg tcggcgcttg aatcagagag
     8341 acaggttcat ctttcaatag caagccaacc tctttagcaa aactttcttg ttcctccctc
     8401 acttcaaggt tcaacagaga aatattttta gagaagtctt gagatagttt tcgtagctcc
     8461 agagaagctg ccgttttctt aaaatacaga ccttgaactt ggaccaagtc tggagaactc
     8521 aggatagatt ggttgcgata agtttcctca ataaccaggt aggactcata taggagagcg
     8581 tcagccattt ccagcaagcg ttccaagaga cctttaggaa gctgggtcat cttttcccgt
     8641 aaaaaaagaa gtaattctgc tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta
     8701 agaggaattc ctaattctcg acacaaaatc ggcaagctat atttttccag ttcagggaaa
     8761 aagacctggg ccaattcgac cgtatcaaca cgagagtttc ttagctcata gccttcaaaa
     8821 aataaatttt ccgccaagag attagcatca aactgaacat tatgggctac aaaaatccca
     8881 tcctccacca agtcaaatat ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga
     8941 cgttggtctg tcaatcctgt cagttctttg atatgagcat ccaagggttc atgtggattg
     9001 acatccgtcg tatagtgatc gacgatttct ccgtcctcaa tcacgacaat tcccacttgg
     9061 ataattttag ccttactacc tgtgctagtt gcctctaaat ccaccacaac atagcgatta
     9121 ccaatcgttt tcattataaa aaattatacc aaaaaaaggg ccatttggca cctgcaaaca
     9181 taggataatt ttcaaactca agaacactac ttttcttaaa aatcccatcg aatagagcat
     9241 tctagcctgc tgatttttgt aaaatagtga agaaatacga aaaattgaga gaagaaatgt
     9301 ttgcatcttc cttctctcaa cgaatcatgt attttatcta accatgtcag gatcgtagtc
     9361 ataaaatcct gtatcaagga aacggttttt agggtgaagt ttacaaactt cttcatccag
     9421 caagaagact tgatcatggc taaagttgcc ctcttgaatc aagttacgcg cttggataaa
     9481 gagttttgca atctcaacaa agttctgacc aggagtgtag agcccttcta gtttccagcg
     9541 agtaaagcca tgctccacca attctgttaa tttgatcatc aaatcaaggt cattgttggc
     9601 aaagatatgg gtcccatgat tatcttcaaa aatggaatag tggctctctg gatcacttgg
     9661 ctcagccaag aagaggtcac gtttatgcgt cttttcatca tcgatatgtg taaagttata
     9721 gtagttttgc aagagtggac gtttagaatg atggatgacg ctagcaccgt aaaccaaaac
     9781 ttcagcagga atttccaaaa tctctggcat tttgaaaagt tcagctgatg gaatttcacg
     9841 cgccaaaaca gcctcagatg cgccagcctt ttgtccccag aagttaatct gacgactgct
     9901 agttaccatg gttgaagcat cgtagatggt cttaaatgaa taaccatcgc ggttaactac
     9961 gtaaaagacg cctgcatccc caatcgtaat atagtctgtc ttgatttctt ccaagaagtc
    10021 taagaaaggc ttgatacggt ccatcatatc ttggtgcatg agagcattga ccgcaacgag
    10081 caattcctta ccagcatcat gaaccaactt agcgatttca cgtaattggt cataactaaa
    10141 ggtcgttggc agacgaaggc caaaatcttt ctcacctaca tagatacggt ctacgccagc
    10201 ttcgagtagt tgttcaactt gttcaatact ttcagcagtt gctgtaatga caatcttttc
    10261 cataagaaaa attataccac atttctccaa aatcagccat gttttgaaaa tgaaaaggga
    10321 ctttattctt tttgtaacct ttctgtaata attctctgcc gaaaaaatga taaaataggt
    10381 atgtactgtt aaggagagaa tcatgcccgt aagaaaatta caatcctatg aggtagacta
    10441 tcaagaagaa ttaaaccagc agcttcctca ttaccaagct tatacacctg aagcgcaatc
    10501 tgatgccaat ctcaaggaaa tcctattttt tattaatatc gctgtttttt gtatctgtat
    10561 tgctatcttt agttttatct ttttagcatt aaaattatca actgctcttg cctttgccgc
    10621 agcaatcgga ttcagcttac ttgttttaaa agttcaacgg tctattatca aacgaaaacg
    10681 tagaagataa atccaaaata tcgccccaat tggagcgata tttttatttt ttctttttag
    10741 atggtgtctg aatggcaatc ttcacttcaa agattgttcc cttgggttta ttatctttga
    10801 cagtaacagt tccttttaga gcatctacaa tttgcttggc tagggataat cctaatccaa
    10861 aaccaccttt ttgccgggtt ctagccttgt ctactcgata aaaacggtca aaaattttct
    10921 ttttatcttc tgtcgaaata ccgattccat tatcagaaac aagtaaataa agattgcgat
    10981 cggtcgccga gataagaaaa tcaatttcac catcctcctc agtatacttg acggcattat
    11041 cgaaaagaat ggtcatcagt tgtttcagaa gaagctgatc tgtgacaatt gttcgatgga
    11101 tacgattttc aaaacggaag acacgattat tttccgaagc aatcatctca tagtttgtga
    11161 aagttgtatt aaaaaagcta gttggaactt ctgcaagctc cggcttaatc ccatcatctc
    11221 tccgagctaa gttcagcaag cttgtcgtta aaaaacgcat atttcggact tcttccaaac
    11281 tcgatgcaat gctttcgctc acatccataa tggtagcttc tggcttacga aaaagggtct
    11341 ctaagcgatt ttgcaaaact gcgagtggag ttcgtaactc atgactggca ttttccacaa
    11401 aagactgttg cttctgcata ctctcaagca ggggcctaac actgacccta gctagataga
    11461 gactggcaag taaagacaaa atccagaaac tagccatcac gaccacaatc aattgctcat
    11521 gcttttgact agcctgctcc aactgactgg tattaatcaa gacagcagca tacttgatat
    11581 tggttgaaac cgaactaata ttggtctcca tcaaaatcac acgatagatt tcttcctgcc
    11641 catagctatt aaaaacctga atctggtaga tatgtcctag ttctttcttt tctaacttaa
    11701 tcttatccaa gcccaaaaat cgatttccag aaagaagttg agtaaagtct ttatcaaaga
    11761 gaatgacttc cgtattggaa ctgacattag gttttatttc tactttacta gcgtccgccc
    11821 tagcattttc taaatcttta atctcttctg ttgccctatt tatagccagc tgaataactg
    11881 cttgaggatt ttcactcagt ccatgaagct tatcgtccac cgaagtataa agactcgaat
    11941 gcatgacttg taaaataatc agagtcattg tagaaaaaat cagggtgaag acaccgaagt
    12001 tgcggataaa ataactaaag tcatccgcat accatgtttt tttaagttta ctgaacatct
    12061 tttaaaagat acccaacact acgcaaggtt tgcaaattct ctgcaaaagt ggttcccttt
    12121 aatttcttac ggacttttga aacatagact tcgacaaccg aaatcgttgt atcactatca
    12181 aatccccata gacggtcaaa aatctgcgtc ttaggcaaaa tcacattttg attttgaagg
    12241 aaataaacta gtaaatcgaa ctctttcccc agcaattcga caggagtatc ttcaacttta
    12301 acggtattgg ttgataaatt aaccacgata ttcccataag tcaaggtgtt ttcattaaac
    12361 ttccctgaac gtttgagaag ggcctgaatt cgcattttaa gttcttctag gtagaaaggt
    12421 ttggtcagat aatcatccgc tcccagttca aatccatgtc ccttgtcatc caaactttcc
    12481 ttggcagtca taatcagaac tggtgtcgta attccctttt cacgcaattc ttttaagact
    12541 tggaaaccat ttttttctgg caacatcaaa tccagcaaaa tcaagtcata gacaccactc
    12601 tcagcttcgt agagaccttc ttctccatca aatacctgca taacatccgc aaaatcgtct
    12661 aaaaagtcaa atactgaatt tgacagacct aggtcatcct caaccaataa gatttttatc
    12721 atgagaaact cctccttatt aaaactatta taccaaattt gccttaaaaa aaactcaact
    12781 ctctgcattt tacatgagat agctgagttt tctttttatt ttaggcttat ttatgcattt
    12841 ccgtattgaa gaacaactgc ttcgactgca gctttttcac ggctaatcaa gtcaacacgt
    12901 gctgcaattt ccttgattcc cataccgatg ttacggctaa gagcaaggtc agaaagttgc
    12961 ggctcaaaga actccttgta ttccgccaag cgttgctgag tcttaaatac atgagcagga
    13021 aggataacaa agctatcaaa gctcatatct cctccaaggg ctgccttaat ccaagcccag
    13081 ttttcacgcg cccaagacca agctgttttc tgagttgctt gatgagctag gaattggtaa
    13141 taccaagcag acaagtcctg tggtttgacc acaaatttgt ccttccaaga agtaatcaag
    13201 ttttggatat tatccgcatc tgtactgtat gcaagagctg ctgccaactg gcgtttaaag
    13261 acagcatctg ttgcgtgagt ataagtatca agataaagtg ctaacaagtc tttagtctca
    13321 tgatgtttca tctcattaat cagaacttgt gaacgaatag ctgctgggag ttctgcaaga
    13381 ttctccttgt gtgttgcgaa gatttggcta gcgacttgac gagcttctgc atcatttgag
    13441 cgaatcatca tcgaaacagc cagctgacga accaattcat cctcatctga ttctccgtct
    13501 ttagcttcaa aaccaagacg gtcatagtta tgacgagcca atttagcaac cagtcctttg
    13561 aaggctgttt cagcatccgt tccttcatca ataaagcgct caagggctga aatcacttga
    13621 gaaacagctg aaaccaccag ataagactct tccttagcaa gtttatcaag aactggaagc
    13681 aagtctgcat aagaaatgtg ccctgcctca gccaacaaac gacgttcttg aacaatttgc
    13741 agtttgcttg tgttatcaag tgtctctagc tcagcaagaa cagcttctaa caagtctcct
    13801 tgatagtcgg taatatagtg ggcagtattt tcagtgttga gacgaagagc tccttcattt
    13861 tcagcaagaa gagctgcgta gccagggatt tcgatacttt cagtttcgag tgtatcaggc
    13921 aagcctttcc agttgctatt gaggggcacc acccagagac ggttcttgtc ttcgttctca
    13981 ccgatgaaga attgtttttg tgaaatcttc aagacattat tttcaacttt gacagtaaga
    14041 actgggtaac caggctgttc caaccaagaa tccatgaagg ctgcgacatc acgtcctgac
    14101 gcttgaccaa gggcatccca aaggtcacta ccaatggtgt tgctgtattg gtgtttttca
    14161 aagtaggcat gcaaaccttt agcaaaatca gcatctccta gccaacggcg aagcatgtgc
    14221 atgagacggc ttcctttggc atagacgata gcgccgtcaa agagtgtatt gatttcatct
    14281 ggatgtttaa cttcgacgtg gacagactga acgccatcag tagcgtcacg ttcaagagca
    14341 agaggtactc cacctgtttg gaaatcttca aagatattcc agcttggttc gatggtatcc
    14401 acacagacgt attccatcat attagcgaaa ctttcattga gccaaaggtc atcccaccat
    14461 ttcatagtca cgaggttccc aaaccattgg tgagccaatt catgggccac aacaagggca
    14521 acttgttgac ggctagcaaa tgtagagttc tcatcgacaa ccaagtaaac ttcacggtag
    14581 gtcacaagac cccagttttc catagcacca gctgagaagt caggaagggc gatgtggaga
    14641 gattgaggaa ttggatactt aactccatag taatcttcgt aaaactcgat agagcgaaca
    14701 gcgatatcca gtgagaaatc aagatttgaa agtggatgtg ctttggttga gtagacacct
    14761 accagggtac catttttagt tttagcggtc accccttgca aatcaccagc aacaaaggcc
    14821 aacaagtaag aagacatgcg aggtgttgtc tcaaacttcc agatacctgt ttccttacgg
    14881 ttttcaacat cgatttctgg catgtttgac aaggccaatt caccttctgc ttggtcaaag
    14941 cgaagagaga ggtcaaaagt tgctttggct tcaggctcat ccacacatgg gaaagcttcg
    15001 cgcgcaaaat ggctctcgaa ctgagtagac aagacctcct tcttgactcc atcaactgta
    15061 taataagaag ggtaaatccc tgtcatgttg tctgtaattt taccagaaaa ggcaagaacc
    15121 aattcaactt gaccagcctc agccaattcg atatgaaggg cttcattgtc atggtcaact
    15181 gtaaatggac gagcttgacc tgcaacttct acagaggtga tttccaagtc tttttggtgg
    15241 agggagatgc ggtcactctg tgcttgacca gtgatggtca ctttcccaga aaaagtcttg
    15301 gtctcacgac tcaaatctaa aaataaatca taatgttcag gaacaaattg cttaataaaa
    15361 tgttcaactg cttgcatagt tttctcctat tctaagttta agagctgctg ctcttcttca
    15421 aacaaactta ttatatcata ttttgcttga aaaaaaagga ttggacagcg atttccaaaa
    15481 ctttcttcct tctaatagtc tacagtgggt agaccttgcg aaattcttct aaaacaacct
    15541 tgctttcaga tgtaaaagtc agccctactt cacccaaaca gtctgtgaaa aagtcctcat
    15601 gaacctggcg ccaataggca agtgatttgt ctccttcacc ttccttaaag gcatgttgcg
    15661 cagaaacttg attgaagagt tcaacagaaa cctttgtaat ttcgacaatg cagacagact
    15721 gattttgact atctaaaatg atatcgaagg tccctacttg gggaagtggt tcgtcttcta
    15781 gtacatagag gtcataggct gatgctgttg ctgtcttttc tcctttaaac accaaatccg
    15841 ctaaaagatc tggttcaact ccaaaagccc aagcatcgat ttcatctccg atcaaaggat
    15901 tgatttgctt gtatttattc cacatttctt gcggtatcat gggtactcct ttgtaatttt
    15961 ttactttctt cttttatgtg tttaagatga tctggatggt caatctctaa atcaaaaatc
    16021 tctggaatag aactgtagtg gataatgcac ttgataccca actgattcat tttttgtatg
    16081 aaagaagtat tcagatagcc tgctacagca aaatcaatct tgttctttct tgctttatcc
    16141 tgcatatctc ttagcatatc taacattatt ggactttcca tatcatgcca ttgactgttt
    16201 ctcatagtcg caaaaacaaa ggaagtcaaa tcattcattc caactacaat ctttgaaatg
    16261 cccgtttcca gtatactaga taagtcaaaa tacgctgacg gtaattcaat catcgttccg
    16321 actttcccag taaaaccctg ctgacgcaat actgtaatag cttgttttaa ttggtcgtca
    16381 tcattgacaa aaggaaagat aacagataga ttggggttgg tttgataaac ttctgtaacg
    16441 acatgtgctt cagcctgaaa ttcatccaaa cacgccagta aacgcctggt tcctctatag
    16501 ccaaacaagg gatgcccttc gtcaaaaaac tctctagtcc ccactaaaca attggcttct
    16561 gtattcgtta attcagaaaa acgataccaa acttcttcat ctgagtacag ggagcagata
    16621 gtctctagat aatcttttac aaattgctga caacttgcta atatgatgtt ttgattaagc
    16681 tctctcaaca aatattcccc acgaatcatg ccgacgtggt gaaatagttg aggataaatt
    16741 ttttcaagaa ttttttcgcc actaagggca agttgatttc tcatcattca ccttccaatt
    16801 catgtaagaa gtcttgtcca gttctggaaa tcctaataat tcagacttaa ccttcaagac
    16861 taatggcgat gcattttctt ctgtaatctc ttgaatatcc atccaaatat atccaagtga
    16921 atcattcgca ccatcagaca cagcttccga aatcgtaact tgaggtgcac tctcattcat
    16981 tagacacagc ttccgaaatc gtaacttgag gtgcactctc attcatttca acatcataca
    17041 aggccatgac atggtgaacc ataaaatttg ttaactcttc tctgacgaaa acatcgtaga
    17101 ttcgaggatt agagtagctt ctaacagtaa atcccgtctc ttccataact tctctagtca
    17161 gcgtttccgt cagtccttca ccaagttgct gactgcctcc aggtagatca taccgatgtt
    17221 gataagggcc tctcgttttt tcaatgcaaa gtaactttcc attttcaaag caaacacagt
    17281 agaccccaaa gtgatttttg atttccatcc aactcctcct acttcaaaga ccagccacca
    17341 tctattgtca agatttgtcc ttgcatggcg ctcgcttttc cacttgctaa aaaaagacta
    17401 agctctgcta tttcctctgg ttcaatccag cgcttgattg gtgtttcact agccacccag
    17461 tcagctaagc cacttggttc aaaatccgca gcggtcatag ctgtcttgac tgctcctgga
    17521 gcgataccaa agacctgaat cccagcttca gcatagtcta gagccaactg cttggtgaag
    17581 ccagccaagg catgcttgga tgaagtatag gcgtgaccac ctccacctgc taggctagaa
    17641 gcaatggaac acatattgat gatgattccc tttttatttt ccagcatttg tgtcaaataa
    17701 taccgagtca actctactgg aataatgtag ttgatttcaa aaatctcttg aatgtcctgc
    17761 gccgtttgtt ccaacagtgg tttgtaatca tccaaaactc cagcagtatt acacaaaaca
    17821 tccacctgag ggcaccagtc aaaaataggt tccaagtcca aggtcaaatc tctctgtaaa
    17881 aagtgaaaat caccctctaa gagtggcttt tcaccttggt caactccata aacttgatag
    17941 cccttctcta aaaagaggcg agcttgagcc aatccgatcc ctgaactcac tcctgtaatg
    18001 agtacacgtt tagtcatgca cttctaccca atccgttgcc aaaacatcac aaactgtcgg
    18061 gctccacatg gaaaaacctt ctccttcgcc agaaacgttg attaggaaat aaggtgtcat
    18121 ttcaagtgca agcccatttt gctcgatggt atcaaagagt tggacatagt tttccgcacc
    18181 tccccaacca gttcgtacat attttctctt agcctttaac ccaggcagga tctcttcaaa
    18241 tgtcatgttt ttctccttta attctacatt cttcatttaa ttatagcaaa aaaccgcttt
    18301 atacggcttt ttgaatgtga gttattcaaa cctgctacta cttacggcaa attattccct
    18361 gcagcaagat aaatttcata ccattctttt cttgttaagc taaagtttgc cgctcggcta
    18421 acttctctca agtgcttagg atttgttgta cctacgactg cctgcatttt tgctggataa
    18481 cgcaatatcc aagaaatggc aatagttgaa gaggttactc catatttaat agctaaacga
    18541 tcaagtactt gatttaaagc ttgaaatttc tcatttccaa caaaattccc tttaaaatac
    18601 ccgaattgta agacagacca tgcttgaatg accacatcgt gtaattggca atattcaaaa
    18661 atgctgccat ctcgcatagc tgcttgacta tcttccatat taacatgaaa agctgattca
    18721 aatcctggag taaaagccgc actcaattgt agctgattaa cagctaacgg ctgcttgaca
    18781 tcttttttaa gcaactccat catcatagga ttttgattag aaactccaaa atctcgaact
    18841 ttaccttgtt tataaaggag attaaaggct tctgctactt ggtcagattc catcaaagca
    18901 tctggtcgat gaaggagcaa gctatctaga tgatcaatct tcaatctttg caaaataccg
    18961 tctactgatt ttataatata gtccttagaa aaatcaaaat aggtaaattc ttcaatgcga
    19021 atgccacatt tggactgaat ccacatcttt tctcttaaat ctggacgatt ttttaggaca
    19081 agacctaaca gttcttcaca acgaccacga ccataaatat cagccaaatc gaaggcattg
    19141 attccaacag aaagtgctgt ttctacaagc tcttcaactt cttttacaga cttatctttt
    19201 attctcatca ttccgagaac aatttctgat aattctttgt catcttgacc aagagttatg
    19261 tatctcatca aatttttctc ctttaatttc taacattctt cccttcatta taacaaaaaa
    19321 ccgctttgca acggcttttt gactatactt cactccattt tatcttctta aacccacgga
    19381 acaagacaaa gattccaata aagaggacag ctaaaggaat aacttttgta aggaaaacat
    19441 ttgaaattcc catccactca taataacgga gcagagaacc caccacaaga tgggcaataa
    19501 tcatactgac aaatggacga aagaccgctt ctttccaatt ccaaataccg ataactagcg
    19561 aaatcgtaaa gacagacaaa ctatcccagg gagccggaat ataaaaggct ccttcttgta
    19621 tgaagcttgc cattcctaca tatcctaaaa caactagaag aactatagtc ccaacaacaa
    19681 tgtaagtgcc aattttcatt ttaggagaat cttggactaa acttcttcgt aaaattgtgg
    19741 ccacaagtcc aaatccaatc agaaaaataa gaagttgccc taaaaatgtg agcaaattga
    19801 ctgttaagag aggaccttta gaaaaatcac ttagtagttg ataataacgt aataccgcca
    19861 ggacaagaat tggcgtcaaa agggactctt tgatagaact gcgaggtgct cccttgagaa
    19921 tctctttcat tattttttta ggattcttac ctagataatc ctctgcactc atgccatctc
    19981 gttctgcttc tgagaaatct agcatcatca aatagatctg ctctctgaga tagtcttcat
    20041 catagagaaa tccagcaaga ttaaaacttt cccacaactc ctcaaaatac ttttgattct
    20101 cctcagaaaa ctcatgtagc aaagcgcttg tttcttcgta atacttcatt ttcttcatgg
    20161 tttaaccccc attcttaatc ccttctactt tttgactcaa atcgtcccat tgttgccaaa
    20221 agactgagac acgctcttct ccttctttca ttaatgaaaa atacttccga tctggaccat
    20281 ctggcgacgg gcgcatgtcg cctcttatcc attgattttt ttctaacttt tgcaacaaag
    20341 gataaatagt tcctggaacg atagtatcaa atccagcctc tcgcaaagtc tgaaccaact
    20401 cataaccata ccgctctttt tgaccaatca tatccaagac acaaccttca agaacacctt
    20461 ttaatagctg agtttctttc atcacttctc ccttctaatc tattttgtaa tacctactag
    20521 taacttcacc tatagtatat cacttctaca ctagtttgta aagcataata gttaatactc
    20581 ttcgaaaatc tcttcaaacc acgtcagcgt cgccctaccg tatgtatggt tactgacttc
    20641 gtcagtttca tctacaacct caaaactgtg ttttgagctg acttcgtcag tttcatctac
    20701 aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc ctagtttgct ctttgatttt
    20761 cattgagtat aaataaaaaa acagaactag cctgaactag tcctgtctac ttttacccaa
    20821 tcacacttcc atttggtaca gctggatcaa ctgtgagaag ggttaatttg ccatcatgtt
    20881 cagctgagag aatcataccc tggctgacat attttttcat cattttacgt ggtttgaggt
    20941 tagcaatgat ttgaactttc ttgccgacca attcttgttc atttggatag tattttgcaa
    21001 ttcctgaaag aatctgacga tcttctccat caccagcatc caagcggaat tgaagcaact
    21061 tatctgaacc ttctacttta gacacttctt tgacttctgc gacacggatt tcaaccttgt
    21121 caaagtcttc aaacttgatt tcatccttgt ttagtttgag ctcaacttcg tccggattcc
    21181 attctttttc gactgctggt ttattgcctt ccatttgttc cttgatatag gcgatttctt
    21241 cttccatatt tagacgtgga aagataggtg ttcctttggc aactacagtc acatctgctg
    21301 ggaagtcagc caaactcaag ttttcaagac tagaaacttc ttccaaacca agttgagtca
    21361 aaactgcacg actagtttcc atcataaatg gttcaatcaa gtgagcaact acacgaatgc
    21421 tggctgccaa gtggctcatg acacttgcca attggtcacg aagagcttca tccttggcca
    21481 agacccatgg tgcggtctca tcgatgtatt tattggtacg agagatcaga gtccagactg
    21541 cttcaagcgc acgtggatag tcaactgctt ccatgtgtgt atggaagtct gcgattgatt
    21601 gttctgcaac ctcagcaaga acatgatcaa attcagtcac accttctaca taggcaggga
    21661 tttgtccatc aaagtactta ttaatcatgg aaaccgtacg gttaaggagg ttcccaaggt
    21721 cattagccaa ttcatagttg atacggccga catagtcttc aggagtaaag gttccgtctg
    21781 aaccaactgg aaggttacgc atgaggtagt aacgaagtgg atctagtcca taacgctcta
    21841 ccaacatttc agggtaaacg acattccctt ttgacttaga catttttccg tctttcatga
    21901 caaaccaacc atgggcaatc aaacgatcag gtaatttaac atccaacatc ataagaagga
    21961 ttggccagta gatagagtgg aagcgaagga tatcttttcc taccatatgg aagactgttc
    22021 cattccagaa cttgtcaaag ttaccatgtt cgtcttgagc gtagccaaga gctgtcgcat
    22081 agttaagaag ggcatcaatc caaacgtaga caacgtgttt tggatttgat gggacaggca
    22141 ctccccatgt aaaggttgta cgagataccg ccaaatcttc caaacctggc tcgatgaagt
    22201 tgcgtagcat ttcattaagg cgaccatctg gcgtgataaa ttcaggatga gctttgaaaa
    22261 attcgaccaa acggtcttgg tatttgctaa ggcgaaggaa gtatgattct tcagaaaccc
    22321 attcaacctc atgacctgat ggagcaatac caccagtcac atttccagct tcatcacgga
    22381 aaacttctgc cagctggctt tctgtaaaga attcttcgtc tgatactgaa taccaaccag
    22441 agtattcacc caagtagata tcatcttgag caagtaagcg ttcaaagacc tgtgcgacaa
    22501 ctttttcatg gtagtcatca gttgtacgga taaatttatc gtatgagata tctagtaatt
    22561 gccagagttc tttaactcca accgccattc catcaacata ggcttgaggt gtaataccag
    22621 cttcttccgc tttctgctgg attttctgac catgttcatc aagacctgtc agataaaata
    22681 catcgtagcc catcaggcgt ttgtaacgtg ctaggacatc acatgcgata gttgtgtagg
    22741 cagaaccgat atgaagtttc ccagatggat agtaaatcgg cgttgtaata taaaaatttt
    22801 tttcagacat aatttttcct ttccaggcaa atgaaacctg tttttctaac acttcattat
    22861 atcacatttt taatgaattt cgatagggaa atccatacaa aaacaagata gacgagtgtc
    22921 catcttgttg atctcattca taacgaaggg cttcaattgg atcaagtttc gatgccttgt
    22981 tggctggcaa gactccaaaa atcataccaa cactagccga aactgcaaga ctaaataggg
    23041 caactgggat tgatactcca acttctatac cttctattaa accttgcagt aacaaacctg
    23101 ctaaggcagt taaaccactt gcaattgtca agccaattaa gccacctaac aaggtcaaaa
    23161 tcatggattc aatcaaaaac tgaattaaaa tattggcacg tgttgcgccc aaagccttac
    23221 gaagaccaat ctcacgagtg cgctctgtca ccgaaaccag catgatgttc atgacaccag
    23281 ttcctccaac aaagagagaa atccctgcga tggaactaat aatcgtcgtc ataaaactaa
    23341 acgattgttg aatttctgca aatacaacgg actcatctgc cacctggtat tctccctgtt
    23401 gtaagcctgc aagctctgtc atttttcgtg ccagttctgg acccagagtt ggggttaaac
    23461 tggtatcatt cactcgaaag acaatactag ctatttcatc tatattaaaa ttcgcagcaa
    23521 gggagatatt ggtagtaata ggcaagccac caaacccata tatttttgac cttttagcct
    23581 ccggactagt ataaacccca atgacccggt aactaaatcc attgacttct acaaccttgt
    23641 taatagcctc ttgaggagat tcaaataaac taatggacaa ttcctcatct agcaaaataa
    23701 cacttgcaaa ctctttgaaa tcttgctctc tcagactacg acctgcaata atttcattct
    23761 taacagcgtc catgtaagtt ctgtttccac ctgtcaaatt agcattctca acctttttat
    23821 cttgataggt caagatggca ttcgttgaat tggttacata gtaactatcc actcccttca
    23881 gtttagctgc ctcttggacc caggattctt gcggttttgg cggttcaaca ggaatttcct
    23941 cttcctttcc agaaaccgta aaagatgatt gtttctgagt aaaagaacca tctttacttt
    24001 ttttaggaga gaaaaagacg ctaatatttt tctgagattt agtcatatct ttattgactt
    24061 gacgagatag ggaatcaccc aaagccataa tcacaacaac tgatgaaaca ccgataataa
    24121 tcccaatcat agtaagcaaa gaacgcatct tgtgagccat gatagatgaa aaggcaaatt
    24181 tcagattctg catcttagtt ttcctccttt cctaactgag cactgtcaga cgaaatgacc
    24241 ccatcccgaa tgacaatctg acgtttagca taggcagcaa tctcaggctc atgcgttacc
    24301 atgataatgg tttttccttc tttattcaaa tcaaccaata attgcataat ttggttacct
    24361 gttttggtat ccaaggctcc tgtcggttca tccgctagga taatagaagg attgtttacc
    24421 aaggcacgcg caatggctac acgttgcttt tgaccaccag ataattctga aggtaaatgg
    24481 tgactacgtt ctgtcaattc aaccttgtct aaatattcct cagccaactt gcgacgtttt
    24541 gaagacgaaa ctcctgcgta aatcaagggc aattctacat tttgcagagc attgagcttc
    24601 gatagaagaa agaactgctg aaagacaaaa ccgatttgtt ggttacggac cttagccagt
    24661 tgtttttcac caagcccagc cacttcttga ccttcaagat aatattctcc actggttggt
    24721 gtatccaaca tgccaatcgt attcatcaga gtggacttac cagacccaga tggtcccatg
    24781 atggctacaa attcaccctc attcacttct agattgatat ttttgagaac ctgcagttct
    24841 tggtcaccat tacggtagct tctgaagata ttttttagac taattagttg cttcatcagc
    24901 cttcacctct tttccttctt ccaaggaaga tgttggatta ctgatgacct tagcaccatt
    24961 cgttaaacca gaagtgattt cttgattttc tgcgtcagca tttcccaatg aaacctcaac
    25021 ttttttagcc ttttgttgtt catccacaat ccagacataa tttttactat catccattac
    25081 tagactgcta acaggaacaa gaatagcctt agttttgctt ttaacctcaa tgttgacaga
    25141 aaaaccctgt ttcaaatcac caacctcgcc tgtcacatca atagtataag ggtatttaga
    25201 acctgtatta ttcccggctg ctggactagc tgcttcacca ttgtttttag gatagtcaga
    25261 aatatagctt aatttcccag tccatttttt atcaggatac actttagaag taaagcttac
    25321 ttcttgacct acagaaaggt tggctagatt gtactcagac aattctccct tgacttgtaa
    25381 attttcattg ctgacaatat gaaccataac ttggctcgcc cctgttggag atttagaaac
    25441 attgctattg acttcgacta cagttccctc tagggtactg agaacagttg ttgcatccaa
    25501 ttgactttga gccttgctta attgcgccgc agcatctgca cgcgcatcac gggcatcacc
    25561 caattgagca tcaatagaag caacagaatt tccagccact ggagttgggc tttgcaccgt
    25621 tgcatcttct cctcctactg gcgctggtaa ctgtggagcc ggagctgaag cggcttcatt
    25681 tcgtgcttga ttgagttcat tgatatgaca atctgcccta gctactgctc gactagctga
    25741 atcataggcc gcctgcgctt ctgaactact gtacttgact aaagcctgcc cttcgctgac
    25801 cttatcgccc acagaaacaa ggatttcatc taaatcaccc ttactagcat caaaataaac
    25861 atattgttca ttttttgctg ttactgtccc tgacaataaa acagaggagg ccacgcttcc
    25921 ttccttggca acaacaagat gagtaggctc atcttttaga gcagtctgag aaggttgtct
    25981 aaagagtaaa atccccccgg cacccaatac aactacactc gcagcaccga ttgctgcata
    26041 cagttgccac tttttagctt taccattctt tttcttcata atgaaactcc ttttcttttt
    26101 tacaatactt tgctattata ccaaatttcc ctccagcaaa caatgcagtt cagatggaaa
    26161 taatggcagt tcaggattaa acaatcgttc ggaattttgc ttttctattg tactagatat
    26221 ataatacacc atcttgactt atttggcaag cttttttgca attttttgtg cgtagcagat
    26281 ttttgcaatc aaatcaaaaa aagaataaaa tataactatc aaaaaagacg ctctactatt
    26341 taaaagatta caaaggagtt ttcaatgaga gaatatgata tcattgctat cggtggaggt
    26401 agcggaggaa tcgctaccat gaaccgtgct ggtgaacatg gagccaaagc agccgttatt
    26461 gaggaaaaga aattaggtgg aacttgtgtc aacgtcggtt gtgttcctaa aaaaatcatg
    26521 tggtacgggg cacaaatcgc tgagactttc catcaatttg gagaagacta cggctttaag
    26581 actactgatc ttaactttga ctttgcaacc ctacgtcgca atcgtgaatc ctacattgat
    26641 cgcgctcgtt cttcttatga tggtagtttt aaacgcaacg gtgtagactt gattgaaggt
    26701 catgctgaat ttgtagattc tcatactgta agcgtaaatg gtgaactgat tcgtgctaaa
    26761 catatcgtga ttgctacagg tgcccatcca agtattccta atattcctgg tgctgagcta
    26821 ggtggctctt ctgatgatgt atttgcctgg gaagaacttc cagagtcaat tgccattcta
    26881 ggcgctggtt atatcgccgt tgaattagct ggcgtactcc acacttttgg tgtcaagaca
    26941 gatctctttg ttcgccgcga tcgtccttta cgtggttttg attcctacat cgttgaaggt
    27001 ttggtcaagg aaatggaaag aacaaactta ccacttcaca ctcacaaagt ccctgtcaag
    27061 ttagaaaaaa ctactgacgg cattaccatt catttcgaag atggtactag tcacacagct
    27121 agccaagtta tctgggctac aggtcgccgt ccaaacgtta agggcttgca acttgaaaaa
    27181 gctggagtga ctctgaacga acgtggcttt atccaagtgg atgaatacca aaatactgtt
    27241 gttgagggaa tctatgctct aggtgatgta acgggcgaga aagaactgac tccagttgca
    27301 atcaaggccg gacgtaccct atctgaacgt ctctttaacg gaaaaactac tgcaaaaatg
    27361 gattactcaa ctattccaac tgttgtcttt tcacaccctg ctatcggaac tgttggtttg
    27421 acagaagagc aagctattaa agaatacggt caagaccaaa tcaaggttta taaatcaagc
    27481 tttgcatcta tgtactctgc ttgcactcgc aaccgtcaag aatcccgttt caaactcata
    27541 acagctggtt cagaagaaaa agttgtcgga cttcatggaa ttggctacgg cgttgatgaa
    27601 atgattcagg gatttgccgt tgctatcaaa atgggagcaa ccaaggctga ctttgatgca
    27661 actgtggcaa ttcacccaac tgcatctgaa gaatttgtaa ccatgcgtta atcgaaacaa
    27721 aagaagctga tataaatatg tctgcttctt ttttaatgat tttcgaatca acaaaaaaat
    27781 tatccaactt gactagtcac gaaaatcggc tgttacagtt tttgttacct tgtttttaaa
    27841 aaatggttga catcgactcc tatatgagta taatagttac tatacattag aaaagaggtt
    27901 aactattttg aaaaaagctc acgtttatgc tatccctgct attggggctg ctctcattgc
    27961 tgctttggca caaatcagtc ttccaattgg acctgttccc ttcactctgc aaaactttgc
    28021 aattggcttg attgctactg tctttagacc gagagaggct gtactttctg ctggactcta
    28081 tcttcttcta ggtgctatcg gtcttcctgt ctttgcagga ggtggagctg gttttcaggc
    28141 tttagttggc cctactgcag gctatctttg gttttatctc gtttactctg gacttacttc
    28201 ctctctaacc aacagcaaga gtggtgttgt taagattttt cttgcaaacc tcttgggtga
    28261 tgcccttgtc tttgtcggcg ggattctcag cttgcatttc ctagctggaa tggcatttga
    28321 aaaagctctt gctgtggggg ttcttccctt tatcattcca gaccttggca aacttctggc
    28381 tattagtttt attagccgtc ccctacttca acgccttaaa aatcaggctt actttactaa
    28441 ctaaaaaagg atatcgagtt atcatgactc aatatccttt tcttttattt tgaaaactta
    28501 tactcaatga aaatcaaaga gcaaactagg aagctagccg caggctgctc aaaacactgt
    28561 tttgaggttg tggatgaaac tgacgaagtc agtaaccata catacggcaa ggcaaagctg
    28621 acgcggtttg aagagatttt cgaagagtat tagttgcctt taaaggcatc caccatcgtt
    28681 tgaaattctt catttgagag agtaatccct ttgcccattt tagtatggtc tggactccaa
    28741 gcacgaatat caaactttgc aggggcacca ttaaagctca cacggttaat ttccttggtc
    28801 caaccttttt cgttttcaga aagagtcaac aagtgctctt cgatttcaaa tgtaaattct
    28861 gccattttct tctccttttt tagtttcatt agtttattcg taaaatcttg tagattttag
    28921 gaaaatttta tataatattg atataaaaga agggaggcca atatgagaca taaattccag
    28981 caagttctaa ataaaataca tgatttttta aatggatatg accaacctga ccagactgaa
    29041 accaactccc ttacagccac tattgaagag gctatccaga aacaaaccgc tgttcacctt
    29101 atcttgtctg agacaagctt tacaggtgac atcatcaaat atgatcagca aggccagcaa
    29161 attatcgtga aaaatttttc caaaaatgtg agccggatta tccgtataag cgatattcaa
    29221 cgcctgcgat ttgtcccctc aactgtccaa acagcccaaa aaaatagatt taagaaagag
    29281 tgagatgtag ttgcttcatc ccactctttt tcttagcgaa tttgttcaaa atgtaaatga
    29341 actgcaatat gatctccata accacttctt tccaagtcac gttgtaaacg ataggaaatg
    29401 tagtgttctg caatggtaat gtaacctgcg cccaataaac gatgttcaac catagattga
    29461 atcatactga tagtcgcacg ttccaccttg gcttcttgta aatccaaaac taccttctta
    29521 gtgacttgag caagattttg acgcaaatca tctgtcaaaa cataaacagt ttgggctgcc
    29581 ttcaagatgg cttggtaaat cttatctgga ttaaattcag caatttcgcc attacgtttg
    29641 attacttgca taggtttctc ctttattctt tgttttcttt gatttctgcc agcatttttt
    29701 cttcttctac tgtcagttga taatgttcaa gtaaatctgg tctgcgctcg taggttttct
    29761 ttaaactctc gtacaatcgc cactgacgaa tcttttcatg gtggccactc atcaatacat
    29821 ctggcacgac catgcctcga taatcatagg gacgtgtgta ctgaggatat tctaaaagac
    29881 ctgaagaaaa actatcatct tggtggctag actccttgcc aatcacttct ggaatcaggc
    29941 gaactgtagc atcaatcatg gtcatagctg ccaattctcc accagtgagg acatagtcgc
    30001 ctagggaaat ctcatctgtt accaaggtct taatgcgctc atcataaccc tcatagtgcc
    30061 cacagataaa gattagctct tcctcttgag ccaaatcttc agcataagcc tgatcaaact
    30121 gctttccagc aggatcgagg agaataacgc gcggattttt cttttcaata gcatcaaagg
    30181 aatcgaaaat aggttgtgct ctgagcaaca taccctgacc gcctccgtag ggctcatcat
    30241 ctacatgacg ggccttttca gcattttctc gaaaattatg atactggata tccaagagcc
    30301 ctttttctcg agcctttcca acgattgagt gctccagtgg agaaaacatc tctggaaaga
    30361 gggttaaaat atcaatcttc atcgtctaac ccttctaaga tttccacatc gacccgttta
    30421 tttggaatat caacattgag aaccactggt gggatataag gtaaaagcaa atcacgtttg
    30481 ccttttcgtt tgaccaccca gacatcatta gcacctggtt gcaggatttc cttgatggtt
    30541 ccaaccaagc tatcaccctc atagacttcc aaaccgataa tctcgtgata gtaaaattca
    30601 ccatcgtcta ggtcattcaa atcttcctca gcgaccttga gactatatcc cttgtacttt
    30661 tcgatagtat tgatatggta catatctttg aatttaataa tgtcaaagtt cttctgttta
    30721 cggtggctag cgatggtcac tgtttggaca aactgatctt tttcatcaaa caaagccagc
    30781 tcagctcctt ttttaaaccg ttcttctgca aaatccgtca cagacaagac tcgcatctca
    30841 ccctgtaatc cctgcgtatt aacgattttc ccaacattaa agtagttcat cttgtctcct
    30901 gtaatctcct tttttccatc ttattctgac aattctcgaa taatagccgc aattttttcc
    30961 gattctgacc attgtaaata atggtgattc cctcctaaaa tgagtttagt attggaagtc
    31021 caatattctg attctctgta ctctttttct ctataaggct gacaaaaaac aaatacagga
    31081 atatgagctt ctatagatac atcctcaaaa tcttcctcag taatctctcc agatatctga
    31141 aattctggat cttgattttc caactctaag cctttttctt gcatcaattc ccagattttt
    31201 ttattcgttt caggactaaa tgttgcttga gttaagttct taaaataaag ttcaggacca
    31261 cactcgtcaa tcagcctcat ctgctcttcc atttctggat aaggattttc tgaaaaatca
    31321 gcaaacatga cttttttagt tgtcggttca attgctacta aagtctgaca cttaattggt
    31381 ttctcgagta atttgcaagc taaaattcca ctccaactat gtgcacaaag tatatattca
    31441 gaaatcccta attcttcaag tacttcataa accacatctg caagattatc tagatttttt
    31501 ccagcttggt catgaatcgg actcctacct gtgttcggaa aatcaattgt caaataacca
    31561 attgtaggag gaggtttttc aagtataagt gaaaaatttt cataacttgg tagcaaacct
    31621 gctccgttta gacaaactag cactttcttt tgcttttgat aagcaacaga gagactacca
    31681 atttctgtag atacttcaaa cctcttcata aagaaatcca ctcattctat ataatgaatt
    31741 attaaaaatc cttatccttt attttatcac gttccaagga ttttctcaag ttggaggaag
    31801 gggacaacat ctctactttc ccttcaaaaa tccttccaaa tcatgtttat gttggtaatt
    31861 aacggctgcg gttttgtctt tctcaaagac agtcttggta aggtcaatat gattaatagc
    31921 tacgattgcg acggtgtagt aaatgatatc agccagttct ctggcaagtt cctcgttcga
    31981 atcctatccc ttcttttcga ccagagcgcc tattcaaaac ctcggctact tctccgactt
    32041 cctccactaa cttcataaag agaccttcat cagtccgaga ctgctgttaa tgttcgagta
    32101 agtagtcttg gaattgccta aacgttcaat cttttatagt atattgaaac tagaatagta
    32161 cacctttact tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcctg
    32221 ttattatttc attttactat atcttctatt ccacacaaaa aagcgagaca tccgtcccgc
    32281 ccttcttatt tttcgtcaat aacgattctt acttttttgt attcagttgg gacagagtag
    32341 acaatcgttc ttatcgcaga aatagtgcga cccttacgac cgattacacg acccacatcg
    32401 ctttgatcaa gattcaaatg atattccaaa aattctggtg tatcttcaat cttgatagtt
    32461 aaggcatctg gttgtgaaat taagggtttc acaatcgcaa taatgagatt ttcaatcgta
    32521 tccatctgtc aacctacttt aaacttattt tgagaattta gaatcgtgga attttttcaa
    32581 tacgccttct tttgaaagga tgttacgtac tgtgtctgaa ggttgagctc cattagccaa
    32641 ccatgcaaga acgcggtctt ctttcaaagt tacttggttt tcagcaacaa gtgggttgta
    32701 agttccaact gtttcgatga aacgtccgtc acgtggtgaa cgtgaatctg ctacgttgat
    32761 acggtagaaa ggttttttct tagaacccat acgagtcaaa cggattttaa ctgccatttt
    32821 taaagtctca tttctttaat tttttatttc ggtgaaatag ctgagctatt tagcacatgt
    32881 tctattatag cagatttctg gcaggtgtca agaaaaaaac ttgacagact ataaatttct
    32941 taaagcattt aaaaatttgt tagaatagga aataaatgtt tgaaagatgc tatccgtgaa
    33001 tactatttta gaaaaatttt ataaagaaca ccaagtcaaa cccttcatat cgcctgaacg
    33061 agatttggat acttggcttc taagtcccaa gcccgttccc aagcgaaata tggacttatt
    33121 agtagatgat tcactagcag gagatatcat tttactatgg agaatccagt ttggtacttt
    33181 taccactgaa acatgatttt aaaaactccc attcgaatat gattcaaaat tcgcttagaa
    33241 tcaatgtttt cccacttttt caaaaaataa attttcatct aatttaaata actttcaatt
    33301 aagtgtgaat tttgcttaaa atcattaaaa aacaccccat ggtgtgaacc atgaagtgtt
    33361 gtaaatgctg tgttgtagct agttcttagc gtttagagaa ctggctagct ttacgagctt
    33421 tcttaagatc tggtttccaa aatactttga gtacacttat ggtattgatg cccctaaaca
    33481 tctaaaaact ctggtagaaa aagggtatgc tcttgtcgaa acttcttttg actcacttga
    33541 ccatctaaat actaccatga aaaagaatat cctgaagaag ggagttactg gactttctaa
    33601 aatgaaatct tgcgacctgg atcaagccct tcatgagcat ttttcagaag aagaattagc
    33661 tggtcacttt catgtccttc tatggacttt tttacaatgg cattgctatc acacccaata
    33721 cctatctaag cgcctggttc gtaaacttta ttgcagctct tcctctaaat ttcctaattg
    33781 ttgaaccaat tgcccgtttt atactaagtt cttttcagaa accatttact ggggaagaag
    33841 ttgaagattt tcaagatgat gatgaaatcc caactattat ctaagccagt tctgtaaact
    33901 actaatattt gaaatccact tccttttagg gtacaatggt ataaatgaat ttttgagagg
    33961 atcagaatga aaaaactagc aacccttctt ttactgtcta ctgtagccct agctgggtgt
    34021 agcagcgtcc aacgcagtct gcgtggtgat gattatgttg attccagtct tgctgctgaa
    34081 gaaagttcca aagtagctgc ccaatctgcc aaggagttaa acgatgcttt aacaaacgaa
    34141 aacgccaatt tcccacaact atctaaggaa gttgctgaag atgaagccga agtgattctc
    34201 cacacaagcc aaggtgatat tcgcattaaa ctcttcccta aactcgctcc tctagcggtt
    34261 gaaaatttcc tcactcatgc caaagaaggc tactataacg gtattacctt ccaccgtgtc
    34321 atcgatggct ttatggtcca aactggagat ccaaaagggg acggtacagg tggtcagtcc
    34381 atctggcatg acaaggataa gactaaagac aaaggaactg gtttcaagaa cgagattact
    34441 ccttatttgt ataacatccg cggtgctctt gctatggcta atactggtca accaaacacc
    34501 aatggcagcc agttcttcat caaccaaaac tctacagata cctcttctaa actccctaca
    34561 agcaagtatc cacagaaaat tatcgaagcc tacaaagaag gtggaaaccc tagtctagat
    34621 ggcaaacacc cagtctttgg tcaagtgatt ggcggtatgg atgttgtgga taagattgct
    34681 aaagccgaaa aagatgaaaa agacaagcca actactgcta tcacaatcga cagcatcgaa
    34741 gtggtgaaag actacgattt taaatcttaa aaaccaaaaa aaatacagta tccacattcg
    34801 gtactgtatt tcttttactc tcattcttaa gttaaattat taaaatccca tatttggtct
    34861 atccagcctt cataaaagtc tagctcgtgg cagaccataa ggatagatcc cctatattct
    34921 ttgagagcgc gtttgagctc atcctttgca tccacatcca aatggttggt cggctcgtcc
    34981 agcactaaaa cgttgttttc acgattcatc aagagacaga aacgaacctt ggcttgctct
    35041 ccccctgata atacttgaat ctggctttca atatgtttgg ttgtcaaacc acaacgggca
    35101 agggctgcac ggacttctgc ttgattaagg gcaggaaagg cattccagac agcttcaaga
    35161 ggagtttggc gattaccgcc ttctacttcc tgttcaaaat aaccaagttc taaataatct
    35221 ccacgctcca cttccccagc gattggcgag ataatgccca agagactctt caagagagtt
    35281 gtttttccaa taccattagc accaataatc gcaacctttt gattgcgttc gaaggtaaga
    35341 tttaaaggct tagtaagagg acggtcgtaa ccaatttgca agttcttggc ttggaagata
    35401 aagcgccctg gtgtacgagc tggtttgaaa tcaaaggatg gttttggttt ctcactttgg
    35461 agttcgataa tatccatctt atccaatttc ttttgacgag acatagccat attacgagtt
    35521 gcaacacggg ctttattacg agccacaaag tccttgaggt ctgcaatctc tttctgctgg
    35581 cgttcgtagg ctgcctctag ctgagatttc ttcatagcat aaacttcttg gaactggtag
    35641 tagtcaccag agtaacgcgt cagctgttga ttttccacat gatagacaat attaataacg
    35701 tcattgagga atggaatatc gtgcgaaatg agaacaaagg cattctcata gttttggaga
    35761 tagcgcttga gccaatcaat atgctcagca tccaagtagt tggtcggctc gtccaacagc
    35821 aagatatcag gcttttcaag gagaagtttt gccaaaagca ccttggttct ttgcccacct
    35881 gacaaagaag ttacatccgt atccatgcca aagtccataa caccaagagc acgcgctact
    35941 tcgtcaatct tagcatccaa ggtatagaaa tcacgactct ccagacggtc ttgaagttct
    36001 cctacttctt ccatgagagc atcaacatcc gcgccgtctt cagccatttt catatagagg
    36061 tcattgatac gagcttcagc tttgaaaagc tcatcaaaag ccgtacggag aacatcacgc
    36121 accgactgtc tttcagcaag gacagagtgc tgatccaagt aaccagccgt cacatatttg
    36181 gaccactcaa cctttccttc atctggcagc attttaccag tcacgatact cataaaggtt
    36241 gattttcctt caccattggc accgaccagg ccgatatgtt ctcccttgag gagacggaag
    36301 gacacatctt caaaaattgc acggtcacca aaaccgtgac tcagattttt aacttctaaa
    36361 atactcattt taattcctta tcttgttttt atgtaatcgt ttataaagga gccaagccag
    36421 atagccaccc aaagtgttgg tccacaaatc atcaatctca aagacgcgat tgaaatcaaa
    36481 gaaaaagtct aagattaatt gcgtacactc gattccaaga ctcacaagaa aactaaaaag
    36541 aaggaccttt tttgttttcc gcaaatttgg aaatagataa aggagttgga aaatcagagg
    36601 aaaaaacaag aagacattga ggatattttg taaaaaaatc caacataatt gtccaatgtc
    36661 actcacttcg cccagtttcc agagagaatt gaaaggagtc aaaagaaaaa ccaggcgtcc
    36721 aagatgctgg atacctggag ttcccactcc cacggtagat tgttcttgag gagtaaagca
    36781 aaaacagaca atgcaaatgc tatagaaaat gactccccag accaaaatat gattataagt
    36841 cttcttcatc attaaggatt taccgctgcg actgccttct ggcggtcacg tttcattgtg
    36901 ttagagcgca attgtccaca agctgcgtca atatctgtac catgctcttg acgaaccaca
    36961 cagttgaccc cttttttctt aagcgtatca tagaaagcca acacgcgctc tttgggacta
    37021 cggctatatt ggtcatgctc actaactggg ttataaggaa tcaagtttac ataagacaat
    37081 ttcttgatgt tcttgagcaa ttcagtcaat tccaaggctt gttctacacc gtcgttgact
    37141 tcattaagca tgatatattc aaaggttaca ccacggtttg ttgtctcaat gtagtattca
    37201 atagcagcaa agagtttttc aatcggaaag gcacggttaa tcttcatgat acttgaacga
    37261 agttcattgt taggtgcgtg aagagacacg gcaagattga cctgaacccc ttcatcagca
    37321 aagtcacgaa ttttatgagc caaacctgag gttgaaaccg tgatgtgacg agcaccgata
    37381 gccattcctt tatcatcatt gatagtacga aagaaattca agacattgtt gtaattatca
    37441 aagggctcac cgattcccat gacaacgata tggctgacgc gttcatcctg accacgctca
    37501 gcaaagtatt tctgaaccag catgatttgc gctacgattt caccgttatt gaggtcacgt
    37561 tgcttcttaa tcaaaccaga ggcacagaag gtacaaccga tattacagcc gacctgagtg
    37621 gtcacacaga cagataaacc atagtgttga cgcatgagta cagtctcaat taacataccg
    37681 tcgggcaatt caaagagata tttgactgta ccatcagcag actcttgcac aatacgttgt
    37741 ttcaagggat tgaccacaaa ctggtcattg agcttagcaa tcaaatcctt ggaaaggttg
    37801 gtcatttctt caaatgactg cacacgttta cggtagagcc attcccagat ttgatctgca
    37861 cggaatttct tttctccctg ctccaatacc cattcctgca tggtttgatg tgccaaacta
    37921 tgaattgagg gtttcatttc ttctccttat tctctactca cttctgacga atgacaaaat
    37981 gacgttgtcc cttgtcctct ttctgagaat gctggtcttt ctgacgacgt ctatttttct
    38041 tatctgcatt cgactttcgt ttagtttgag tcggtttctt tccttttcta gaaggtgttt
    38101 cttcttccgt cttacgcatt ttcttgtcaa atgatgctcg cttaggggct tcattttcta
    38161 agacaaaata ggcacaacca taactacaat actctaaaag gtagtcttgt aaacgactga
    38221 ttttttcaag tttttcttct gttcggtcat ccttgtaaaa acctcgtagg cgaagctgtt
    38281 cgttgctcca gtcccccacg atataatcaa acttggttaa tacttctgaa aaacgctgat
    38341 taaaagtcgt cacatcaaag gcatccttga tattttcaac caaggaaaaa gctatccctt
    38401 ccgtttcgac cttgtccccg tgtaaatgga actccggacc aggaaacttg ttatagttgt
    38461 ataattcagg tgcaatttct tttcgcatag atatcctttt ttcacgatta cttaatactt
    38521 tattctacca taatttctag cagttagcac gtttctcata aaaatgaaaa aagtctgacg
    38581 attttgtcag accagaatct tataacctaa aaagagaaga acaattcttc cctccaacta
    38641 tcattattta gcagctgcgt acaattcatc tactttattc cagttgatta ctgaaaagaa
    38701 agctttgatg tagtcaggac gcacgttgcg gtatttcacg tagtaagcat gttcccaaac
    38761 gtccaagccc aagattggtt ttttaccttc tgagattggt gtgtcttggt ttgctgttga
    38821 agtcacttca agtttccctt ctttgttgac aaccaaccat gcccaacctg aaccaaaacg
    38881 agttgttgct gctgcagtga aggctgcttg gaattcttca aatgaaccaa atgttgcatc
    38941 gattgctgct gccagttctg ctgaaggagc tgttttctcg ggagtcatca attcccagaa
    39001 aagagcgtgg ttcaagtgtc cgccaccatt gttgataagt gcttgacgga tatcagctgg
    39061 gatagattct acatcagcaa gcaaggcttc aaggtcttca ccgatttcag ggtgtttttc
    39121 taaagctgca ttggcattgt tgacataagt ttgatggtgt ttgtcatggt gcaaatgcat
    39181 tgtttccgca tcgatgtatg gttccaaagc gtcgtatgca tatggaagtt ctggtaagat
    39241 aatagccatc tgtaatacct ctttttcttt ctatatgaaa atgataacgc aaacattcac
    39301 ttttttcaag ttttttgctt atagataagg aaatcactga catactttct aataaaataa
    39361 gcaaatcagc aagaaaccca cgacaagata gtcctgacgc gggttgtagt ttcaacaaat
    39421 gtcaattgac ctgactagca atctgtaaga gtgccttttc aaaaaggaaa cctttttcat
    39481 aaagacctgt cttaatctga tagtctgtct caatcaaata ggaaatagct tgcttcaaaa
    39541 agctcaaaga aagtcctctc gaatctctta atgcaaactt gatttgataa ggatttgggt
    39601 tacgtcccag ataactacct aaactacttg caatctgcga ttctgtttgg ccagactccg
    39661 ccaaaatctt cacctgagta aaagtccgaa attgtcccag catgactgca atcagtttga
    39721 tttcatcttc cccttgcaag gtcaagtctc tcaccaaatc gcgcgcctga tccatctttt
    39781 tagtcagaat aaactgagtt aaatcaaaaa tattgtcctg caaggtcttg ggaattgcgt
    39841 taacaatatc ctcttcctca ataacagaat tcgccttata ggactgtaaa aagagaagat
    39901 ttttctggat ttcgctaaat tgaaaccccg acttgatgag gagattttca aaagaatgat
    39961 tggtaaactg cagaccttgt ttctgactcc acttttggaa atactggcgc aattcttgtt
    40021 cttttacttc tactgcatcg aagaccttgg catcacgctt aagtaatttg actaaccgtc
    40081 ttttgctatc cagctttcct tctgcaaaga ttatcaactt ggttgttgga gaagaattgt
    40141 caaggtattc ctcaaatgac ttaagctcat catctgtcaa aaagcgtttc ttagcagtcg
    40201 tgatatccat aaaataatct aatatcacga ttttctcatc cgcaaagaaa ggaagactga
    40261 ccaactccag ttccacatcc ttgtaaacta cttctttcat atcaaagtag gcaaagttga
    40321 ggtcagcaga atcataccca atctgtttca acacttgact cttcatcact tcaaactgac
    40381 cctgatctgt ccctgtaaat aggctcaggc tcggtaaatt tgataaagtc aacttctgac
    40441 tttcttcaat ggctagcatc gtctctcctt tcttcagatt tttcgattta atttagtcaa
    40501 tatagcgcaa tttcccacgg aaatcttcta agctctcgta gcctttttcc accatgattg
    40561 ctttcagttc attggtaatg cggtcaaaag cactgacgcc ttctttgtga agggtcgttc
    40621 ccacctgcac catacttgct ccacagagga tgtgttcaaa ggcatctcga ccagtcagaa
    40681 cgccacctgt tccgataatt tggatttgag gatttaaacg ttgataaaag gcgtgaacat
    40741 tggctagagc agtcggtttg atgtattctc caccaattcc accaaaacca ttcttaggcc
    40801 gaataacgac agattcgtct tctatataga ggccgtttcc gatagagtta acgcagttga
    40861 caaacttgag cggatatttg ttgaaaatag ctgccgcttg atcaaagtga acaatatcaa
    40921 aataaggtgg caatttaatt ccaagaggtt tggtgaagta agcaaacact tctgccaaaa
    40981 tccggtctgt tgtctcaaaa tcataggcaa tctgaggttt acctggaaca tttggacagg
    41041 aaagatttag ctcagtcaga ccacgaaaat cactctcttg gacttttttc aaaatagtat
    41101 gggtttcctc tggagacatg ccgaccagag ataagaagaa agttcggttc gactcttttt
    41161 cctgcaaatc caaaagataa tccaaataat agtctaagcc attatttggc aagcccatag
    41221 agttgatgga accaagtgga acatcttggt agcgtggctc aggattcccc tgacggaagt
    41281 ccaaggtcgc tgtcttagta acaaaggttc ctgccgctga gtttttgacc tcttctaact
    41341 cctctatcgt catacaagcc acacctgctg cattcatcaa gcaattgtca aactcaaaac
    41401 cagcaatttg tgttttcgtt gataccatga ttctaatcct ccagatttct tttattgcta
    41461 atattatacc ataattttaa ttctaggttt actagattct gtggttctat ctatttattc
    41521 ggaaaagaaa gtctagtaca gaagtcatgc tatggaaaca gctagtgtat tagaagtttg
    41581 aacaaggctt gggtaacaga cttttgaatg aagtccaagc aacaaggaga aatctcaacc
    41641 atgagtccat tccatgactc tgttgctcta gaaaaccttg caatagtcaa gtgataacga
    41701 ataaaacttt ggattgaaat atccccaaga ttatctatta cgaggagaaa tcttaacctt
    41761 tatcattttt aaagaaatat agtaaaatga aacaagaaca ggagaaatcg atcaggacag
    41821 tcaaattgat tgctaacaat gttttagaag ttgaggtgta ctattctagt ttcaatctac
    41881 tatacatgta aatctctact ttcgcaaaat ggattttcag aacagtatcc cagtatagtt
    41941 aaaaactata aaaaagagag gacgttcccc tcttaaaatc ttattttact gcttctttca
    42001 aagcatctac cttatccaaa cgttcccatg gaagatcaat atctgtacgt cccatgtgac
    42061 cgtaagccga tgtttgacgg taaattggac gcttgaggtc cagcatttgg ataatccctg
    42121 cagggcgaag gtcaaagatt tgacgagccg ctttttcaag ttgactttca gctactgttc
    42181 ctgtaccgaa agtatcgata cgaacagaaa caggttgcgc aacaccgata gcataggcca
    42241 actgcacttc tgccttctta gcaaggcctg ctgcaacgat attcttggca atatagcgag
    42301 ccgcataaga ggctgaacga tccaccttag tcgcatcttt accagagaag gcaccaccac
    42361 catgacgaga gtagccacca taagtatcta caataatctt acgaccagtc aaacctgagt
    42421 ccccttgagg accaccgatt acaaaacgac ctgtcggatt gataaagaat tttgtcttat
    42481 catcaagata agaagatgga ataacttctt tgatgacctt gtcaatcaca tcttgatgga
    42541 tttgttcatt agtggcctct ggatcatgct gagtagaaat aacgactgta tctacacgta
    42601 ccggacggtc attttcatcg tactcaactg taacttgtga ttttgcatct ggacggagat
    42661 agctaatttc tccagactta cgaagttctg ccagacgacg aaccaattta tgactgagtg
    42721 caattggcaa tggcataagc tcttctgttt catctactgc aaatccaaac atgagccctt
    42781 ggtcacctgc tccaatcaag tccagtggat cttgatcagc atttccgcga acctccaagg
    42841 cttcgttaac accttgagcg atgtcaggag attgttccac caaagatggg tgtactccca
    42901 ccgtctcagc agaaaatcca tattctgtat tggtataacc aatctctgca atggtatcac
    42961 gaaccacacg gttaatatcc acataggcat ttgtagaaat ttcaccaaaa acgtggacag
    43021 aaccagtata tacagctgtt tcagcagcaa cgtgcgcctc tggatccttt gctaaaatag
    43081 catccaaaat cgcatctgaa atttggtctg caatcttatc cggatgcccc tcagatacag
    43141 attcagacgt gaataattta cgttctgaca taaaaatgtc cccccttaaa aaatattgtt
    43201 atagagttac aaagtactga taggaaaact cttcgtaata aatacctacc atcttatcgg
    43261 gactatataa ctttatcatt ataccatttt taatcgcaat ttgcagtctt taatgtagtg
    43321 tttcggttta gatagttcgt tctcataaag caaaaagttg gagctcagta ctccaacaat
    43381 ttctattaaa atgtttggcg tttagcttta cgctctgcac gaccgcgagc gcgattttca
    43441 gcacgcttag ttttacggcg cttttcatca accgcccatt gaattttctt cttataaccc
    43501 ggtttgactt ttttcttttt ctttttaacc aaaccaatca tttcgatatc aagtttatct
    43561 tgttttttct cacggttggc acgacgatca cggtcatagg tatcttgaaa ttccccatct
    43621 ttgaccatct taggactaaa cttgattccc aatttctcca actcacggat atccgagtca
    43681 tcacttggct gataaagggt aatagctgta cctggtaggc catttcgtcc agtacgacca
    43741 acacggtgaa caaaaaaaga taagtcttgc ggaatggcat cattgatgac atggctgaca
    43801 ccttcaatgt caatcccacg cgctgctaaa tctgttgcga caatatactc aaaatccaga
    43861 ttttgcacct gattcatgat tcgcttgcgt tcacgagggg caatatcgcc atggattttt
    43921 gcaaccttca agccttgagc agtcagatat gaatgcaatt catcagcacg cgttttagtg
    43981 ttaacaaaaa tcattgccaa atacggctgc atcaactgag tcaactggta aatttgagca
    44041 ttcttatcat gtcccttggt cgaaatcaac caattatcaa tggtgtcaga aataaccgtt
    44101 ttagtcttaa ttttctccat aacaggattt gataagtatt ttttcaagaa tggttgcagt
    44161 ttttgtggga tagtcgctga gaagaccatg aattgcaagt cttttggaag actgccagca
    44221 atcttatcaa cagtttccaa gaatcccata tccaaggtca tatctgcttc atcaacaaca
    44281 aatgtcttgg ctttatgaat agctaaatca ccagatttaa ccaagtcgta gatacggcct
    44341 ggtgttccaa taacaatatg aggctgattg ctttccaatt tctcaatctg gcgagcctta
    44401 tccgtaccac ccacataatt aaccacacga acttcgacat ctgagtgagc tgaaatctga
    44461 cgcgctactt ggtaaatttg agtagccaac tcacgactcg gtgcagtaat cactgcttgt
    44521 acactatcgc tagcttcatc taattgctgg aaaatcggta acaagaaagt gtgagtctta
    44581 cctgaacctg tttttgattc tcctactagg tcacgacctg ccaaaacaat aggaatcaac
    44641 ttgtcttgca cctctgttgg agttgtaaat tttaactcct tcaaggcttc tctaatatag
    44701 tttttaaatt gaaatttcgt aaatgacata acatcctcga ttctatctat cttatcaatt
    44761 ataccatatt ttattccatt acagtagtct cacttattta ggctatttcc agtagcttct
    44821 ctagtaagaa aaggctggaa ttttatagtt ccaacctctt ttcagttatt atttccagtt
    44881 taacatagca ttcaagccat agtgatcact cacttgtgga ctcttgttac catcaaatac
    44941 gacatgtaaa ttttccaccg ctaactcttt ggtagtaaag acataatcga ttcgaagggg
    45001 ttcagtgttc cctttccagc catcaatttc aggcggaaca gtatagctac cacttttctc
    45061 ttgagcaact tcaaatgcgt cttgtaagcc taatggacta gctaaaatag cttggtaacc
    45121 ttcctgacct gctgggttgt taaaatctcc agctagtaaa agtggcttgt tcaatttttt
    45181 caagacaacc tcaaatcgtg cccattcttc ttgaaaacct ttatcccacc aagagagatg
    45241 aacactggca actgctagct ccttgccatc gactacagtt tcagctaggg caacacggcg
    45301 agtatgatag tctgttggat catccacatc tgaaaccaaa atttctctgg cttcaatagg
    45361 tgttttagac aagatagcca caccttcgtg gtagcggtca tagccgatat ggttataggc
    45421 ccaggtccag tagtaatttt tcccttgctc agataacttt tcaaccaaga gtctaacata
    45481 gtgatcttgg tgaataggct cagctgctgg caaagcttga taaaggtcat taacctccac
    45541 ctctgacgag gtcatctcct gattaatttc ttggaaacaa atcaaatcat agtccttttc
    45601 aagaatatct tcaagcaaaa tctggaattt ttcctctgct tctttctcca tccaactatg
    45661 agtattgagt gttaaaaatt tcattctttt ctcccaaaat aagaggttgg aatacagctt
    45721 ccaacctcaa gtatattaca attctacttt agcaactgct gttttagctg caagagaacc
    45781 tgttttttct aatttaactg atttaattgc atcaccattt gtgaagacaa ctactgttga
    45841 agtttcacgt cctgctgcac ggatagcatt caagtcagct gtgacaagga gatctcctgc
    45901 tgcaactttt tgtccttcag caacatgaac tgtaaatggt ttaccttcaa gacttactgt
    45961 gtccaaacca atgtgaacca atacttcaag acctgcttcc gtcacaatac caaaagcatg
    46021 ttttgttggg aagatgcttg atacagtacc tgaaactgga gatacaatgt ttccatttgc
    46081 aggttctact gcaaatccat cacccatcat tttttgagca aatactggat cctttacttg
    46141 ttccaaagca acaacttgac cgtctgctac tgagtaaact tcctcagtaa gatctttgaa
    46201 gtgaacagtg ttttgttgta cttcagtcat ttggcttgga agagtttcag gaatgatttc
    46261 acctgaatca aggatatctt ggatatcaga tttcaaaatg tcagcttttg gaccgtagat
    46321 agcttgaacc ccttgtcctt tcatcacaag acccatagct ccttctgctt tccattgctc
    46381 tgcatttcct actttatctg catctttaac agttacacga agacgagtca tacatgcatc
    46441 aacatcaacg atgtttacac gtccaccaag aaggttgata atgtttacag cttgagagcc
    46501 tgctgcaact ttcacttcgc tgctggtttc ttctgaacct tcagcagttt cgtagtttcc
    46561 gttgcgccct ggagttgcgt agttgaattt ttgaatcatg aagtttgcga taaagtacat
    46621 gattacagca aagagaacag ttacccaaac gaagttaacg atatccatac caataccagc
    46681 actgattgca ataggtgtac gagtcaagaa ctcgattgaa ccgaatgagt gcatacgtag
    46741 gtttacgacg tcagccatag cgaaggcagc accttgaaca agtgagtaaa caagatacat
    46801 aggtgttgcg atgaacatga acatgtattc gattggttca gtaacccctg tcaagaatgt
    46861 tgcaagagct gttgcaatca tcataccttt gtatttatgt ttcttgtcag catcaacatt
    46921 acggtagata gcaacaatca cacccatcaa gataccgaat gaaccgatca tttgtccaac
    46981 tttgaaacga gctggatgta ctgtatctaa caagtgttgg tattgactag catcagtacc
    47041 tttaaggttt acaaggtctg ttacccatgc aagccatagt gggtcttgac cgaatacttg
    47101 agtaccttta gctgcaccag ttaaaatgtc ataagtacca ccaagagctg tgtagttcat
    47161 tgggatagtc aacatgtggt gaagaccaaa tggcaagagc aaacgttcca aagtaccata
    47221 caagaatggt gcaagaattg gagcagtttc ttgtgagttg gcaatccaga taccgaaatt
    47281 attgatacct gtttgaacta ctggccagaa agcagcaagt agaattgcag cgattgctga
    47341 acgaagaata actacaaatg gtacgaaacg tttcccgttg aagaatgaaa gtgcatcagg
    47401 aagtttacgg aagttgtagt atttgttgta agcagttgcc cctacaaaac ctgagataat
    47461 ccctacgaat acccccatgt tcaaggctgg agcttcaaga acactgataa agtaatcagc
    47521 aactttgatt gaaccaccaa agaaagtagt taccatagca tctggatttt tcaacatatc
    47581 gcctgataca ccaaagattg taccagtgat acggttaatc aagatgaagg caagaccagc
    47641 ggcgaaagca ccaccagcac gttctttagc ccagcttcct ccaatggcta gggcaaacaa
    47701 aatgtgaagg ttaccgataa ccccccaacc gatttgctca agaattccac ctgtgatgac
    47761 aagtggtgca aaggttgggt taatcatcac gatagactta ccgattgaaa tcatcaaacc
    47821 agcagccggc ataactgcga taactaccat caaagcctta ccgaattttt gccaaaattc
    47881 gaaagacaag acatttttga atgtatcttt catcattcaa atctccttta tttttattta
    47941 ggcaaacgtt ttacgaaaac gtttgcactt tctatgattt aattatacca ctttaatttt
    48001 ttttgtaaag gcttttataa aaaaattaca cttttttcta aaaaatttct a
//
LOCUS       DAVKJU010000006        46455 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVKJU010000006 DAVKJU010000000
VERSION     DAVKJU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.006"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            97..780
                     /locus_tag="VW191_000267"
     CDS             97..780
                     /locus_tag="VW191_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9623110.1"
                     /translation="MNFSFLPKYLPYFNYGAVETILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            780..1514
                     /locus_tag="VW191_000268"
     CDS             780..1514
                     /locus_tag="VW191_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9623111.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGISLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKAKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            1668..2525
                     /locus_tag="VW191_000269"
     CDS             1668..2525
                     /locus_tag="VW191_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEU9623112.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            2667..3539
                     /locus_tag="VW191_000270"
     CDS             2667..3539
                     /locus_tag="VW191_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU9623113.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRQLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPESMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            3821..4504
                     /gene="rpiA"
                     /locus_tag="VW191_000271"
     CDS             3821..4504
                     /gene="rpiA"
                     /locus_tag="VW191_000271"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEU9623114.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            4518..5729
                     /locus_tag="VW191_000272"
     CDS             4518..5729
                     /locus_tag="VW191_000272"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU9623115.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            5731..6273
                     /locus_tag="VW191_000273"
     CDS             5731..6273
                     /locus_tag="VW191_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU9623116.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELKNLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            6289..7098
                     /locus_tag="VW191_000274"
     CDS             6289..7098
                     /locus_tag="VW191_000274"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9623117.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            7574..8554
                     /locus_tag="VW191_000275"
                     /pseudo
     CDS             7574..8554
                     /locus_tag="VW191_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            8579..9091
                     /locus_tag="VW191_000276"
     CDS             8579..9091
                     /locus_tag="VW191_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEU9623118.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNGETAI
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            9749..10459
                     /gene="deoD"
                     /locus_tag="VW191_000277"
     CDS             9749..10459
                     /gene="deoD"
                     /locus_tag="VW191_000277"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU9623119.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(10482..11006)
                     /locus_tag="VW191_000278"
     CDS             complement(10482..11006)
                     /locus_tag="VW191_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEU9623120.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(11059..11295)
                     /locus_tag="VW191_000279"
     CDS             complement(11059..11295)
                     /locus_tag="VW191_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU9623121.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(11363..12283)
                     /locus_tag="VW191_000280"
     CDS             complement(11363..12283)
                     /locus_tag="VW191_000280"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEU9623122.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            12481..12921
                     /locus_tag="VW191_000281"
     CDS             12481..12921
                     /locus_tag="VW191_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623123.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIAAGSYCFLVLWIATEGYFF
                     RMSLEDLIRLWTSLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            13137..13718
                     /locus_tag="VW191_000282"
     CDS             13137..13718
                     /locus_tag="VW191_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9623124.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETSGDLTIVIQKKQ
                     GAPSAKSKMEGVFGNCEILKKDKGYYILRSVKE"
     gene            13715..14992
                     /locus_tag="VW191_000283"
     CDS             13715..14992
                     /locus_tag="VW191_000283"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEU9623125.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILVPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            15010..15672
                     /gene="deoC"
                     /locus_tag="VW191_000284"
     CDS             15010..15672
                     /gene="deoC"
                     /locus_tag="VW191_000284"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU9623126.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            15659..16048
                     /locus_tag="VW191_000285"
     CDS             15659..16048
                     /locus_tag="VW191_000285"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9623127.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            16138..17190
                     /locus_tag="VW191_000286"
     CDS             16138..17190
                     /locus_tag="VW191_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEU9623128.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            17334..18869
                     /locus_tag="VW191_000287"
     CDS             17334..18869
                     /locus_tag="VW191_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623129.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQGLGVLMAGGNLGKEK
                     SDV"
     gene            18862..19920
                     /locus_tag="VW191_000288"
     CDS             18862..19920
                     /locus_tag="VW191_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623130.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            19923..20879
                     /locus_tag="VW191_000289"
     CDS             19923..20879
                     /locus_tag="VW191_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623131.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            20964..22311
                     /locus_tag="VW191_000290"
                     /pseudo
     CDS             20964..22311
                     /locus_tag="VW191_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(22354..22995)
                     /gene="plsY"
                     /locus_tag="VW191_000291"
     CDS             complement(22354..22995)
                     /gene="plsY"
                     /locus_tag="VW191_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU9623132.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            23131..25074
                     /gene="parE"
                     /locus_tag="VW191_000292"
     CDS             23131..25074
                     /gene="parE"
                     /locus_tag="VW191_000292"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU9623133.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTALKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            25494..27965
                     /gene="parC"
                     /locus_tag="VW191_000293"
     CDS             25494..27965
                     /gene="parC"
                     /locus_tag="VW191_000293"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEU9623134.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLRIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKNFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            28095..29117
                     /locus_tag="VW191_000294"
     CDS             28095..29117
                     /locus_tag="VW191_000294"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU9623135.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            29173..29877
                     /locus_tag="VW191_000295"
     CDS             29173..29877
                     /locus_tag="VW191_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623136.1"
                     /translation="MVLSTLAILVACGKTDKEADAPTTFSYVYAVDPASLGYSITTRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPIVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            29942..30631
                     /locus_tag="VW191_000296"
     CDS             29942..30631
                     /locus_tag="VW191_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEU9623137.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            30628..31551
                     /locus_tag="VW191_000297"
     CDS             30628..31551
                     /locus_tag="VW191_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEU9623138.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            31566..32210
                     /gene="pcp"
                     /locus_tag="VW191_000298"
     CDS             31566..32210
                     /gene="pcp"
                     /locus_tag="VW191_000298"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEU9623139.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            32378..32608
                     /locus_tag="VW191_000299"
     CDS             32378..32608
                     /locus_tag="VW191_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEU9623140.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            32658..32738
                     /locus_tag="VW191_000300"
     tRNA            32658..32738
                     /locus_tag="VW191_000300"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32692..32694,aa:Tyr,seq:gta)
     gene            32748..32819
                     /locus_tag="VW191_000301"
     tRNA            32748..32819
                     /locus_tag="VW191_000301"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32780..32782,aa:Gln,seq:ttg)
     gene            32981..34183
                     /gene="rpsA"
                     /locus_tag="VW191_000302"
     CDS             32981..34183
                     /gene="rpsA"
                     /locus_tag="VW191_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU9623141.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(34269..35595)
                     /locus_tag="VW191_000303"
                     /pseudo
     CDS             complement(34269..35595)
                     /locus_tag="VW191_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            35789..36286
                     /locus_tag="VW191_000304"
     CDS             35789..36286
                     /locus_tag="VW191_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU9623142.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            36286..37941
                     /gene="dnaX"
                     /locus_tag="VW191_000305"
     CDS             36286..37941
                     /gene="dnaX"
                     /locus_tag="VW191_000305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU9623143.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGANTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            37969..38163
                     /locus_tag="VW191_000306"
     CDS             37969..38163
                     /locus_tag="VW191_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEU9623144.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            38313..39083
                     /gene="sufC"
                     /locus_tag="VW191_000307"
     CDS             38313..39083
                     /gene="sufC"
                     /locus_tag="VW191_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU9623145.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            39111..40373
                     /gene="sufD"
                     /locus_tag="VW191_000308"
     CDS             39111..40373
                     /gene="sufD"
                     /locus_tag="VW191_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU9623146.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            40384..41610
                     /locus_tag="VW191_000309"
     CDS             40384..41610
                     /locus_tag="VW191_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9623147.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILIEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATVLDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            41597..42037
                     /locus_tag="VW191_000310"
     CDS             41597..42037
                     /locus_tag="VW191_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU9623148.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            42091..43503
                     /gene="sufB"
                     /locus_tag="VW191_000311"
     CDS             42091..43503
                     /gene="sufB"
                     /locus_tag="VW191_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU9623149.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(43702..44943)
                     /locus_tag="VW191_000312"
     CDS             complement(43702..44943)
                     /locus_tag="VW191_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEU9623150.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            45046..46155
                     /locus_tag="VW191_000313"
     CDS             45046..46155
                     /locus_tag="VW191_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9623151.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT        13958 a         8257 c        10450 g        13790 t
ORIGIN      
        1 tttaaagatt tttaaataaa ttggataaaa aatttaaatt ttaattcaaa acatgttata
       61 atcatacagt attctatttt agaaagcagt gtgactatga atttttcttt tttacctaag
      121 tatttacctt attttaacta tggggctgtt gagacgattc ttatttctat ctgtgttatc
      181 tttttgggaa ctattttggg tgttgtcttg gcttttgggc aacgttcaaa gtttaaaccg
      241 cttgtttggt tggccaactt gtacgtttgg attttccgtg ggacaccgat gatggttcaa
      301 attatgattg cctttgctct tatgcatatc aatgctccga ctattcagat tggaatttta
      361 ggtgttgatt tttcgcgtct gattccaggg attttgatta tctctatgaa tagtggtgct
      421 tatgtttcgg agactgttcg tgccggaatc aatgcggttc caaaaggtca gctagaggcg
      481 gcttattcgc tagggattcg tcctaaaaat gcgatgcgtt atgtgatttt gccacaagca
      541 atcaaaaata tcttgccagc attggggaac gaatttatca ccattatcaa ggacagctcc
      601 ctcttatcag ctattggggt catggagttg tggaatgggg ctacaacagt ttctacaaca
      661 acctatctac ctttaacacc acttttattt gcagcatttt actacttgat tatgacctct
      721 attctgacag tagccttgaa agcttttgaa aaacatatgg gacaaggaga taagaaataa
      781 tgacagaaac cttgataaaa attgaaaatt tacataaatc ttttggaaag aatgaagtat
      841 tgaagggcat cagcctcgag attaaaagag gagaagttgt cgttatcatc ggtccttcag
      901 ggagtgggaa atctaccttg cttcgctcta tgaatttgtt ggaggaagca accaagggga
      961 aggttatctt tgagggagtc gatattacgg acaagaagaa tgacctgttt gccatgcgtg
     1021 agaagatggg catggttttc caacaattta acctctttcc taatatgact gtgatggaaa
     1081 atattacctt gtcacctatc aaggctaaag gtgaaagcag ggaagttgct gagaagagag
     1141 cccaagagct tttggaaaaa gttggtttgc cagataaggc agacgcttat ccacagagtc
     1201 tgtcgggtgg gcaacaacaa cggattgcca tcgcgcgtgg gttggctatg gaaccagatg
     1261 ttttgctctt tgacgaacca acttcagcct tggatcctga aatggtaggt gaggtattgg
     1321 ctgttatgca agaccttgcc aagtcaggga tgactatggt tatcgtaaca catgagatgg
     1381 gatttgcccg tgaggtggca gatcgtgtta tctttatggc agacggtgtg gttgttgaag
     1441 atgggacacc tgagcagatt tttgaacaaa cccaagaaca acggactaaa gatttcttga
     1501 gtaaggtttt ataatctatt ttaaaatttc aagacaagat tagtgaaaac ttcaaccaga
     1561 gctttttctt atagtttgaa actataggat aacctaggaa agaagtgtta gagggcacta
     1621 gcagtttttg gtataatgga agatatttga aagaaaagag aagtgatatg acacagatta
     1681 ttgatgggaa agctttagcg gccaaattgc aggggcagtt ggctgaaaag actgcaaaat
     1741 taaaggaaga aacagctcta gtgcctggtt tggtagtgat tttggttggg gataatccag
     1801 ccagccaagt ctacgttcgc aacaaggaga ggtcagctct tgcggctggt tcccgtagcg
     1861 aagtagtgcg agttccagag accattactc aagaggaatt gttagacctg attgctaaat
     1921 ataatcagga tccagcttgg catgggattt tggtccagtt gccattacca aaacatatcg
     1981 atgaagaggc ggttttatta gccattgacc cagaaaagga tgtggatggt ttccatcccc
     2041 taaacatggg acgtctttgg tctggacatc cagtcatgat tccctcgaca cctgcaggaa
     2101 ttatggaaat gtttcatgaa tatgggattg acttggaagg taaaaatgcg gtcgtaatcg
     2161 gtcgttcaaa tatcgttgga aaacctatgg ctcagcttct tttggctaaa aatgcgacag
     2221 taaccttggc ccactcacga actcataatc ttgccaaggt ggctgctaaa gcagatattc
     2281 ttgtagtcgc aatcggccgc gccaagtttg tgactgctga ctttgtcaaa ccaggtgcgg
     2341 ttgtcattga cgttgggatg aatcgagatg aaaatgggaa gctctgtgga gatgttgatt
     2401 atgaggcagt tgcaccactt gctagccata tcacgccagt ccctggaggt gtcggtccta
     2461 tgaccattac catgctgatg gagcaaactt atcaagcagc acttcggaca ttggatagaa
     2521 aataagagaa acattcgatt aatagagtgt attttctata gctatatcta aaatggcaga
     2581 aatgaatatt aaattttaga tataagatta caaaaggagg tctgcgtctc ctttttattg
     2641 tataataaaa gtgagaggaa aaaaggatga aagtgattaa tcaaacctta ttagaaaaag
     2701 ttattattga acgttctcgt tcaagtcata aaggagatta tggtcgtcag ctgctgcttg
     2761 gtggtactta tccttatgga ggtgccatca tcatggctgc tttagcagct gtaaaaagcg
     2821 gtgcaggatt ggtgaccgtt ggaacggaca gggaaaatat cccggctctg cacagccatt
     2881 tacctgagtc tatggccttt tctcttcaag accagcaatt gttaaaagag caattggaga
     2941 aggcagaagt tgtcttgctg gggcctggtt tacgagacga tgcttctgga gaaaatctag
     3001 taaaacaggt ctttgttaat ttaagccaaa atcagatttt gattgtagac ggaggtgcct
     3061 tgaccattct tgctaggaca agtttgtcat ttccctcaag ccagcttatc ctaactcctc
     3121 accaaaaaga atgggaaaaa ttatctggaa ttacgattga aaagcaaaag gaagatgcaa
     3181 cggctagtgt tctgacttcc ttccctcaag gaacaatttt ggtggagaaa ggcccagcta
     3241 ctcgtatttg ggaagttggc caatctgatt attaccagtt acaggttggt ggtccttatc
     3301 aggcgactgg gggaatggga gatactctgg ctgggatgat cgcaggattt gtaggccaat
     3361 tccgacaggc cagtctctac gagcgtgtgg cagtagcgac ccatcttcat tcagccatag
     3421 cccaagaact atctcaagaa aattatgtgg tcttaccgac ggaaatcagc aggtatcttc
     3481 ccaaaataat gaaaattatt tgtcaacaag agagggtaag caaagataaa cttgtttaaa
     3541 atagacagat aaatctactt ccttctctta atttatgatt cttgaaatgc gcctgcttta
     3601 aggctaaact agacgtatca aagttaagag tttgtttttg taactggaaa gagatgaaca
     3661 aatcaaatac ataaaagata aactcatttt tattcgtttg gtaaaaataa aatgcatatt
     3721 tttaaagaaa gattgatgaa aaatgtagat ttgatgtttt tacttgaata aaaagcggtt
     3781 tcatagtaaa ataagaactg agaatgatgg aggtaaatcg gtggaaaatc tgaagaaaat
     3841 ggcaggtatc aaggctgctg agttcgtgag tgatggaatg gtcgttggac ttggaacggg
     3901 ctcgactgcc tattattttg tcgaagaaat cggtcgtcgt atcaaggaag aaggattgca
     3961 gattacagct gtaacaactt ctagtgtgac cactaaacag gcagaaggcc ttaatatccc
     4021 gctcaagtct attgaccaag tagactttgt cgatgtgacg gttgatggcg cggatgaagt
     4081 ggatagtcag tttaatggaa ttaaaggcgg tggtggtgcc cttctcatgg aaaaggtggt
     4141 cgcaacacca tcaaaagaat acatttgggt ggtggatgaa agcaagctgg tcgaaaaact
     4201 aggtgctttt aaattgccag tagaagtggt tcagtatggt gcagagcagg tctttcgtca
     4261 ttttgaacga gctggctaca aaccaagttt ccgtgaaaaa gacggccaac gttttgtgac
     4321 ggatatgcag aattttatca ttgacctcgc cttggatgtc attgaaaatc caattgcttt
     4381 tggacaagaa ttggaccatg tcgttggtgt tgtggagcat ggtttattca accaaatggt
     4441 ggataaggtc atcgttgctg gacgggatgg agttcagatt tcaacttcaa aaaaaggaaa
     4501 atagaagggg gcataagatg tctaaattta atcgtattca tttggtggta ctggattctg
     4561 taggaatcgg tgcagcacca gatgctaata actttgtcaa tgcaggggtt ccagatggag
     4621 cttctgacac actgggacac atttcaaaaa cagttggttt gaatgtccca aacatggcta
     4681 aaataggtct tggaaatatt cctcgtgaaa ctcctcttaa gactgtagca gctgaaagca
     4741 atccaactgg atatgcaaca aaattagagg aagtatctct tggtaaggat actatgactg
     4801 gacactggga aatcatggga ctcaacatta ctgagccttt cgatactttc tggaacggat
     4861 tcccagaaga aatcctgaca aaaatcgaag aattctcagg acgcaaggtt attcgtgaag
     4921 ccaacaaacc ttattcagga acggctgtta tcgatgattt tggaccacgt cagatggaaa
     4981 ctggagagtt gattatctat acttcagctg accctgtttt gcagattgct gcccacgaag
     5041 acattattcc tttggatgaa ttgtaccgta tctgtgaata cgctcgttcg attacccttg
     5101 agcgtcctgc ccttcttggt cgcatcattg ctcgccctta tgtaggtgaa ccaggtaact
     5161 tcactcgtac ggcaaaccgt cgtgacttgg ctgtatctcc attttcccca actgttttgg
     5221 ataaattgaa tgaggctggt atcgatactt atgctgtggg taaaatcaac gatatcttta
     5281 acggtgctgg tatcaaccat gacatgggtc acaacaagtc aaatagtcat ggaattgata
     5341 cactattgaa gactatggga cttgctgagt ttgaaaaagg attctcattc acaaacctag
     5401 ttgactttga tgccctttac ggccatcgtc gtaatgctca cggttaccgt gattgcttgc
     5461 atgagtttga tgaacgctta cctgaaatta tcgcagctat gagagagaat gaccttctct
     5521 tgattactgc ggaccatgga aatgacccaa cgtatgcagg aacggatcac actcgggaat
     5581 atattccatt gttggcctat agccctgcct ttaaaggaaa tggtctcatt ccagtaggac
     5641 attttgcaga tatttcagcg actgttgccg ataactttgg tgtggaaact gctatgattg
     5701 gggaaagttt cttagataaa ttggtataag atgacgcgct atgctttgct ggtgagaggt
     5761 atcaatgttg gtggtaagaa taaggtcgtc atggcggagc ttcgtcaaga attgacaaac
     5821 ttgggactgg aaaaggttga gagctacatc aacagtggca atattttctt tgcttcgata
     5881 gattccaaag cccaattggt tgaaaagcta gagactttct ttgcagtcca ttatccattt
     5941 attcagagct tttctttact gagtctagag gactttgagg cggaacttaa aaatctacca
     6001 gcttggtgga gcagagactt ggcacgaaaa gattttctct tttacactga gggtttggat
     6061 gtggaccaag tcatcgcgac agttgaaagt ttagagctga aagatgaagt gctttatttt
     6121 ggaaaacttg ggattttctg ggggaaattt tctgaagaat cctattctaa gactgcctat
     6181 cagaagtact tgctgaaggt gcctttctac cgccacatta ctattcgtaa tgctaaaacc
     6241 tttgacaaaa ttggtcaaat gctaaaaaaa taataaagga gacacacaat gacattttta
     6301 aacaaaatcc atgaaactgc tactttcctg aaagaaaagg gaattgcagc ccctgagttc
     6361 ggtctaatcc ttgggtcagg acttggagaa ttggcagaag aaatcgaaaa tccagttgta
     6421 gtagactatg ctgagattcc aaactggggc cgttcaacag tagtcggtca tgctggtaaa
     6481 ttggtatatg gtgaactggc aggtcgcaag gtcttggctc ttcaaggtcg tttccatttc
     6541 tatgaaggga atcctctgga agtggtgact ttcccagttc gtgtgatgaa agttcttgga
     6601 tgtgaaggtg ttattgtaac caatgcagct ggcggtatca gatttggtcc tggtaccttg
     6661 atggctatct cagaccatat caacatgacg gggcaaaatc cattgatggg tgaaaacttg
     6721 gatgactttg gcccacgttt cccagatatg tctagggcct acacaccaga ataccgtgcc
     6781 actgcccatg aagtggctaa aaaacttaat atcaagcttg atgaaggtgt ctatatcgga
     6841 gttactggtc cgacttatga aacaccagca gaaattcgtt cctataagac actgggagca
     6901 gatgcagttg gtatgtctac ggttcctgaa gttatcgtgg cagcccactc tggcttgaaa
     6961 gttctgggaa tttcatgtat cactaacttt gcggctggtt tccaagaaga actcaatcac
     7021 gaagaagttg tagaagtgac tgaacgtgtt aaaggtgatt tcaaaggctt gcttaaagcg
     7081 attcttgctg aattgtaaga aaaaagattt aaaaggggta gtgtctctgt cttttcagga
     7141 ttgactgcct atccgtatta aagaagaaac agaggaatac tatgagcttc ttcctgctct
     7201 tataactgaa agaagcggaa gaataggtat gtctgatctg atagccagca ttgtgaaaga
     7261 caagattcta ggatactagc attagctttc tagccaagca gactagtatg ataaggagag
     7321 atgagaatga attgactttc tgaatttctc agtcttatca tatatagcac aatgagattt
     7381 cgcttgagtc tgcttgtaaa taaacgaaaa gaaagataat aaataatgaa aattggtcaa
     7441 cgaattatgc gctttggcat aaaaaattaa gtatcggagt tgtatctgtt gtagtcggct
     7501 ttgatttcta gctccagctg gaatttcagc caatgaagta aagcaagatg taacatctga
     7561 agtggtaata ggtgtgctag attctaagga ggaattgaaa gagtcagaaa atgatgctcc
     7621 aaaactagaa actcctctta gagaggagcc aagactagct cctcaaacgc ttccggaagc
     7681 aagtgaagtt cttgaaaaca aaagggaaga gtcaaaagta gagataacag agccagctca
     7741 agcggatgat atccgcaagg ttgttgggga attagccaag gatataagta ttactaagtt
     7801 gtatatgaca ggtcattctc ttggatgtta cctagctcag attgcagcgg ttgaagatta
     7861 ccaaaaatat cttgattttt ataaccatgt attgaggaaa gtgacaactt tcagtgctcc
     7921 taaagtgatt acttccagaa ctgtttggga tgctaagaat ggtttctggg atgttggttt
     7981 ggaaagtcgt aaattagctg ttagtggaaa aattaagcat tatgtggttg ataatgacaa
     8041 tgttgtgact cccttgattc ataataatcg tgatattgtt acatttacag gtaattcacg
     8101 ctttaaacac cgttctcgtg gctattttga aagtccaatg aatgatattc ctaactttaa
     8161 tattggtaaa caagctacct tggataaaca tggttatcgt gatccgaaat tggataaagt
     8221 gcgattcttt aagaaacagg ctctgcctcg atcttctagt caaccaagcg ctgaaccaat
     8281 ggaaaatatt gccttaggaa aacaggttac tcaaagttcg acagctttcg gaggagatgc
     8341 tagaagagct gtggatggca aagtcgatgg taactatggt cacaattctg tcactcatac
     8401 aaacttccaa tctaagcctt ggtggcaagt agatttggct aaagaagaaa ccattcgcca
     8461 aatcaatatt tacaactgaa cagacactgc ccaggataga ttggcaaact ttgatgtcat
     8521 tcttttagac agttctggta aagaaattga gtgaaaacgt ataacatctc ctaaagatgt
     8581 gtcagcacaa attacgatta accataaaaa agcgcgctat gttcggattg agctagaagg
     8641 ctataatgcc ctcagtcttg cagaagttga agttttccgc tttatagcta cgaatggtga
     8701 aacggcgata caagtttcta agccagttca accaatcagt cagactcctg tgaaggataa
     8761 aacattgaca attcaacaca gtggagctta cattgcccgc tactccataa cttgggaaga
     8821 agttccagta gataaagatg gaaaccaagt tgttcgtagt cattcttggg aaggaaacgg
     8881 tcgcaaccag actgcaggtt ttgtcctcaa cctcccaatc aaagaaaata tgagaaatct
     8941 gcgagttaag attgagaaaa agacgggcct actatggaat agatggcaaa caatctatga
     9001 aaacagacca attttagctc aaccccaccg taaaattacc cattggggta cgacattgaa
     9061 ttccaaggtg agtgacgatg atgtcttgta atctgatggt agaatgacag ttagtttgtc
     9121 tagtttataa gaaagtacta cctgagcttg aatagaactc aggtagctct ctatgaaaga
     9181 acaaaattaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggttgctt
     9241 aaagcactgc tttgaggttg tagataagac tgacgaagtc agtcacatat ataatccaag
     9301 gcgacgttga cgtggtttga agagattttc gaagagtata aacagaaagg tagagcgcgt
     9361 gttctaattt gaacacgagt agaaaacttt tctaaaaaca aaaacgaaag gatgggtaaa
     9421 ctgtattcgc tgaactgaat acgggcgact ctcctctaaa tcaaaattaa gaaagaaagg
     9481 aattgaaccc accctaaaag cagtgggaaa aagatagttg gtctagcgag catcgctcac
     9541 tgcgcccaac tcctattttc ccttcgcttt ttgatgggtt tggtatcttt ctcaatataa
     9601 aatataaaat aaagaaaggt agagcgtgtg ttttgatttg aacacgagcg gaaaactcgg
     9661 aaaatagata atctgactga aaaatcagga tttctcgtca ggttcctaat tttcagtcgt
     9721 tttcttctcg ctctttgtat cataaattat gtctatccat attgctgctc agcagggtga
     9781 aattgctgat aaaattcttc ttcctgggga tcctcttcgt gctaagttta ttgcggagaa
     9841 tttccttgat gatgctgttt gttttaacga agtgcgtaac atgtttggtt acactggtac
     9901 ttacaagggt cactgtgtat ctgtcatggg aactgggatg ggaatgccat ctatttcgat
     9961 ttatgcgcgt gagttaatcg tagactacgg tgtgaagaaa ttgattcgtg tgggaactgc
    10021 aggttctttg aatgaagagg ttcatgttcg tgaattagtt ttggcgcagg cggctgcaac
    10081 caactcaaac atcgttcgta atgactggcc acagtacgat tttccacaaa ttgctagctt
    10141 tgatttgctt gataaagcct accatatcgc caaaaaactt ggtatgacta ctcacgttgg
    10201 gaacgttttg tcatctgatg tcttttactc aaattacttt gaaaagaata tcgagcttgg
    10261 taaatgggga gtcaaggctg tggaaatgga agcagcagct ctttactatc ttgctgccca
    10321 ataccatgtt gatgcgctag ctatcatgac catctctgat agcttggtca atccagacga
    10381 agacacaact gcagaagaac gtcaaaatac cttcactgat atgatgaagg ttggtttgga
    10441 aaccttgatt gcagaataat tatagccaaa aaggaagccc tttatgaatt gtaggacttc
    10501 cttttcttat ccagaaattg atctagctct ctctgatttc gaaggatagt gactttatgt
    10561 gaatattctt ggcaaagttt ttggtaattt tctttttgag ttttgctacg cccatcccaa
    10621 agaatccatc tgataaactc ccactcaaag cgttcagggc aatctgccgc catactttct
    10681 ctgacttttc cacggtattt aagataacgc ttaaaggctc taaagagaca ggtcaatggc
    10741 aaaaaattga gaaagatgat ttggtcagct tcttgcattc gttcttggta gtagcaccaa
    10801 gaataattac catcgatgac ccaagcttta tgcttggtga gaaagttttt tatctcggtt
    10861 aacatccatt cgcagtcact gtcttgccaa ccaggttgaa attggagtgt gtccatgtgc
    10921 agttttggaa tggagtagta gttagataac ttttctgcta gagttgactt accagaacca
    10981 gaatatccga taattgtgat tttcattttc taccttttcc tatttggaga caaaaaaaca
    11041 gcctctatgg actgtttctt atttagcaag tttagctgaa agacgagctt tatcgcggct
    11101 tgctttgttt ttgtgaatca aacctttagt ttctgcttta tcgatagctg agctagcagc
    11161 acggaaaagt tcttcagatg ggtttgcttc gaaagctttt atagcagtac gcatagctga
    11221 tttttgagct gagttctttt cgttttgttt aacgttcaat tcagcgcgtt tgatagctga
    11281 tttaatgttt gccaatgttc ttacctccat atttactaac tataccatta tatatgaaaa
    11341 cttacgtttt gacaagggaa aattattttt ttaagtaaat ttcatcgatt tcatggttct
    11401 tgcttttatg aagaatcact tctgcacgat ttctggttgg ttcaatataa ttttgtagat
    11461 ttgtgagatt gatactggtc cagacctgat gggcaaaggc ttccacttcc ccaatcggca
    11521 tctgagtaaa acgataatag tagctatcag ggtcgttttg ggctagactc agcattttca
    11581 agaaacggtc cagataccaa ctttcaatat catccactcc agcatctaca tagatggaaa
    11641 agtcaaagaa gtcagtgata tagagacgat cgttttgtgg attttgaaag acattgattc
    11701 cctcaacaat tacaaaatca gcagctttga cactttgttt ctcttcgggt acgatgtcgt
    11761 aaacttcatg agaatagaca ggaatatcta catcttgtcc atttttgatg cggtccaaga
    11821 agttgagaag agcttccata tcatagcttt caggaaatcc tttacgattt aaaatcccct
    11881 gctcaatcaa ggtttgattg ggatagagaa aaccatcagt tgtaaccaac tcaaccgtag
    11941 catctgtaaa cgtacgggac agtaggattt gaagtaggcg actggttgtg gattttccaa
    12001 cggcaacact cccagaaacc ccaataataa aaggttgaga tttactttca cgttggagga
    12061 aaattccttt tgaaaaggct aaatcttcct tagttcgctt gtaaatctga atcagatgag
    12121 ccaaggggag atagatatct gtaacgtctt ggagactgat ttggtcatta aaactcttga
    12181 tagattccaa ttcttcttct gtcaaaggag gtgttgtctt tcgatgtaaa gattgccaag
    12241 tctggcggct gattttttca aaatgtaaaa attcgttggt catttgtttt cccctagata
    12301 ttttgtctat cataccataa aaatctaaaa aaataaagcg gtttcttgaa tgtggtaagt
    12361 caatggtata tagtataagt gatcaattta ttgatgaaat aggagataga ttctactgat
    12421 ttataagttt ataagcttat aaagggggct ttttagttca caacttatag gaggtgtact
    12481 atgaaaatct taaaacgtta catattggaa ctctgtttta ttttaagttt tgctttacct
    12541 tttataaaag gagccaatgc agataatggt agatgctttg tggaaaccta ttacggtttt
    12601 acttttttga tggaacatgc tattgtaaca gctgtcttta tctattcgtt tttaattgct
    12661 ttcttactaa aaaaacgatg ggcgaaatgg attgctgctg gtagttattg ctttttagtc
    12721 ctatggattg ctgctggtag ttattgcttt ttagtcctat ggattgcgac agagggttat
    12781 ttcttccgca tgtcactaga agatttgata cgactttgga caagtttgga aatcctgaca
    12841 caaacgtatc agttgggctt ttatctaaac attttgttgg gaattttgtt gataataaag
    12901 tattttaagg ttaagcaata gaaataataa tctgcctgat tatagatgat aaactttttg
    12961 tttttatctg gactgtgata tttatagttc taaatggttt attatttata agtgttgaaa
    13021 gagattgaag aaaatggctt cgtgactcgc tttcttcggt ctttttcgtt ttattatcaa
    13081 tttgtaaacg atttacaatt tgagtcaaat tatggtaaaa tagtgtcatg agtaaaatgt
    13141 attatgcaga aaatcctgac gctgctcacg acattcatga gttgagagta gagttgttgg
    13201 gacaaaaaat gaccttttta acggatgcgg gtgtttttag caagaaaata gttgactttg
    13261 gaagtcaact attgctcaag tgtcttgagg tcaaccaagg agaaactgtc cttgatgtag
    13321 gctgtggtta cggacctttg ggtttgtcct tagctaaggc ttatggagtt caggctacta
    13381 tggttgatat caacactcgt gccttagatt tagctcggag aaatgctgaa aaaaataatg
    13441 caaaagcgac gattttccag tccaacatct atgaacaagt tgaaggacat tttgatcatg
    13501 tcatttccaa tcctcctatc cgtgcgggca agcaagtcgt tcatgaaatc attgagaaaa
    13561 gtaaagattt cttggaaact agtggggatt tgacaatcgt catccagaaa aaacaagggg
    13621 ctccaagtgc taagtccaag atggagggtg tgtttggtaa ttgtgaaatc cttaaaaaag
    13681 acaagggata ctatatcctt agaagtgtga aagaatgaga gcagttgatt taatacaaaa
    13741 aaaacgagac ggtcaagaac tgacttcaag tgaaattgaa tggctggtag aaggctatgt
    13801 gtcaggaact gttcctgatt atcagatgtc tgcctttgct atggctgttt attttaaagg
    13861 aatgacgact cgagaaatct ctgatttaac gatgaatatg gttaagactg ggcaagagtt
    13921 tgacctgtca gccattgatg gtgtgaaagt tgacaagcat tctactggtg gtgtcggcga
    13981 taaggtaacc ttgattttag ttcctcttgt tgctagcttc ggtgtgcctg tagcaaaaat
    14041 gagtggtcgt ggtcttggcc atacaggcgg aacaattgat aaattggagt ccattaaggg
    14101 ctatcaagta gaacgtagtc aagaggattt tattcgtcag gttcaggata ttggagtatc
    14161 tgtcattggg caatcagacc agctggtcaa agcagataag cttctctatg cccttcgtga
    14221 tgtgaccgca actgtcgaca cgattccttt gattgcgagt tcggtgatga gcaagaaaat
    14281 tgcggcaggg gcggatgcta ttttgctaga cgtgactgtc ggtgagggtg ccttcatgaa
    14341 gacggttgat gaggcgcgtg agttggctca aactatggta gatcttggta aggttgttgg
    14401 acggaagaca gtagcagtga ttacggatat gagccagcct ttgggacgag cgattggaaa
    14461 tcgtctggaa atccttgaag cattggagat tttgcaaggt caaggacgtc aggatattac
    14521 ccactttatt tgtgaattgg ctcaaattat gcttggtttg gcaaatgtta acaaaactgt
    14581 tgaagaagtt cgccaacatc ttgagaatgg tcaagcactg gctaagtttg aggaaatggt
    14641 gcaagcccaa ggtggagact tggaagacct ctatcgtcct gtaaatgtag ctcatgtggt
    14701 ggaaatccct gctcaggaaa cgggtgtcat ttcagctctt ccagctatgg actttggtct
    14761 ttatgccatg aggctgggtg ctggtcgtgc agttaagtct gatgccttgg actatgaaac
    14821 aggaattgtt tttgaaaaga agattggaga ctccgttcaa aagggtgaaa ttgttgcaaa
    14881 agtatacaca aatgaaaaaa ttccacctca actagttaca gatttccaaa aatgtgttaa
    14941 aataagtgat gaagtaaaaa aaatccgtga aattgtagaa atcatctcct aaatggaggg
    15001 aaaaccgaaa tgaaattaaa taaatatata gatcatacgc ttttaaaaca agatgcaaag
    15061 aaaaaacaaa ttgatagttt gttgtctgag gctagagagt atggctttgc cagtgtttgc
    15121 gttaatccga cctgggttga acatgctaaa aaaggacttg aaggcacaga tgttaaggtt
    15181 tgcacagtag taggtttccc tttgggagca acaacttcag ccgtgaaagc atttgagaca
    15241 aaagaagcta tccaaaatgg tgcagatgag attgatatgg tgatcaatgt tggagctctc
    15301 aaatcaggta atttagcctt ggttgagtca gatattcgcg cagtagtgga agcaagtggt
    15361 gataagttag tgaaagtcat tattgaagct tgccttctga cagaccaaga aaaaattgtt
    15421 gtttgccaat tggcccaaaa agctggggct gactttgtta aaacatctac tggtttttca
    15481 actggtggtg ctacgatagc agatgttaca ttaatgcgtg aaacagttgg atctgatatg
    15541 ggagtcaagg ctgctggtgg agctcgttct tatgcagatg ctcttgcctt tgtcgaagca
    15601 ggtgcgaccc gtatcggaac gtcagctggg gtagctattt taaaaggaga attggcagat
    15661 ggcgactact gagttgattg aactggcaat tgaaaccagc aaacatgcct atgtccccta
    15721 ttctcacttt cctatcggag cagttttagt agccaaagac ggaagtgttt acacgggagt
    15781 gaatatcgaa aatgctagct atcctttgac caattgcggt gagagaacag ctatttttaa
    15841 ggctatatct gaaggccaaa gagaattttc agaattgatt gtctatggtc aaactgaaaa
    15901 accgatctcg ccatgtggtg cttgtcgcca agtgatggct gaattttttg aacaagatct
    15961 aaaagtgacc ttagtcgcaa aagataaatc gacggtcgag atgacggtcg gggaattact
    16021 tccatactct tttacagact taaactagtc tgagtcgctc tctgagtggc acggtccttg
    16081 tggccaacca atccatactt gcaacatcgt tgcacatctt atttaggagg ttcagtaatg
    16141 aacaagaaac aatggctagg tcttggccta gttgcagtgg cagcagttgg acttgctgca
    16201 tgtggtaacc gctcttctcg taacgcagct tcatcttctg atgtgaagac aaaagcagca
    16261 atcgtcactg atactggtgg tgttgatgac aaatcattca accaatcagc ttgggaaggt
    16321 ttgcaggctt ggggtaaaga acacaatctt tcaaaagata acggtttcac ttacttccaa
    16381 tcaacaagtg aagctgacta cgctaacaac ttgcaacaag cggctggaag ttacaaccta
    16441 atctttggtg ttggttttgc ccttcataat gcagttgaag aagcagctaa agaacacact
    16501 gacttgaact atgtcttgat tgatgatgtg attaaagacc aaaagaatgt tgcgagcgta
    16561 actttcgctg ataatgagtc aggttacctt gcaggtgtgg ctgcagcaaa aacaactaag
    16621 acaaaacaag ttggttttgt aggtggtatc gaatctgaag ttatctctcg ttttgaagca
    16681 ggattcaagg ctggtgttgc gtcagtagac ccttctatca aagtccaagt tgactacgct
    16741 ggttcatttg gtgatgcagc taaaggtaaa acaattgcag ccgcacaata cgcagccggt
    16801 gcagatattg tttaccaagt agctggtggt acaggtgcag gtgtctttgc agaggcaaaa
    16861 tctctcaacg aaagccgtcc tgaaaatgaa aaagtttggg ttatcggtgt tgaccgtgac
    16921 caagaagcag aaggtaaata cacttctaaa gatggcaaag aatcaaactt tgttcttgta
    16981 tctactttga aacaagttgg tacaactgta aaagatattt ctaacaaggc agaaaaagga
    17041 gaattccctg gcggtcaagt gatcgtttac tcattgaagg ataaaggggt tgacttggca
    17101 gtaacaaacc tttcagaaga aggtaaaaaa gctgtcgaag atgcaaaagc taaaatcctt
    17161 gatggaagcg taaaagttcc tgaaaaataa tggatggaag tcatttctta ggaagcggcc
    17221 aagggccgct tttttaagag ttgagacaaa gtcattttgt ctcagtaaag cttgctacta
    17281 gacaaactag caagttttat tgaaataaaa taagactctg aaaggaagag cacatggcac
    17341 acgaaaatgt cattgagatg cgtgatatta ccaaggtgtt tggtggattt gttgccaacg
    17401 acaaaatcaa cttgcaccta cgaaaaggtg aaattcatgc acttttagga gaaaatgggg
    17461 ctggtaagtc cacgctaatg aacatgttag caggccttct tgaaccaact agtggtgaaa
    17521 tcgcggtcaa cggtcaagtt gtcaatctcg actccccatc taaagcagct agcttgggaa
    17581 tcgggatggt tcaccagcac tttatgttgg ttgaagcctt cacagtggct gaaaacatca
    17641 ttttaggtag tgaattgact aaaaatggtg tgctagatat cgctggagct agcaaagaaa
    17701 tcaaggctct ttctgaacgt tatggcttag ctgttgaccc ttctgccaag gtagcagata
    17761 tctcagttgg agcccaacaa cgtgtagaaa ttttaaaaac actttatcgg ggggctgata
    17821 tccttatctt tgacgaacca acggctgttt tgactccatc agaaattgat gagttgatgg
    17881 ctattatgaa aaatcttgtc aaagaaggaa aatcaattat cttgattacc cacaaattgg
    17941 atgaaattcg cgcagtttct gaccgtgtta cagttatccg tcgtgggaaa tcaattgaaa
    18001 ccgttgaaat tgcaggggct accaatgctg atttggcgga aatgatggta ggacgttctg
    18061 tttcctttaa aacagagaag caagcctcta aaccaaaaga agtggttttg tctatcaaag
    18121 atttggtggt caatgaaaac cgtggtgttc cagctgttaa aaatctgtcc ttggatgttc
    18181 gtgctggaga gattgttggt attgcgggga ttgatggaaa tggtcagtct gaactgattc
    18241 aagccattac aggtcttcgt aaggttgaat ctggtagcat tgagctaaaa ggagattcaa
    18301 ttgtaggctt gcacccacgt cagattacag aactaagtgt tgggcacgtt ccagaagacc
    18361 gtcaccgtga tggcttgatt ttggaaatga tgatatctga aaatattgcc cttcaaacct
    18421 actataaaga accacatagt aaaaatggaa ttttgaatta ttcaaatatt acttcttatg
    18481 ctaaaaagct gatggaagag tttgatgttc gcgctgccag tgaattagtt cctgcagctg
    18541 cactctcagg aggaaatcaa caaaaagcaa ttattgctcg tgaaattgat cgagatcctg
    18601 atctccttat cgttagccag ccaactcgtg ggttggatgt cggtgccatt gagtatatcc
    18661 acaaacgctt gattgaagag cgtgataatg gcaaggctgt ccttgttgtc agctttgaat
    18721 tggatgagat tttaaacgtc tcagaccgta ttgccgttat ccacgatggt aagattcaag
    18781 gtattgtatc accagaaaca accaataaac aaggacttgg tgtcttgatg gctggtggaa
    18841 acttgggaaa ggagaagagt gatgtctaaa aaattacaac aaatttcggt tcccttgatt
    18901 tctgtattcc taggaatttt actcggagcc attgtcatgt ggatcttcgg ttatgatgct
    18961 atttggggct acgaagaatt gttctataca gcctttggca gtctgcgtgg gattggagaa
    19021 atcttccgtg ctatgggtcc tctggtcttg attggtcttg gttttgcggt tgccagtcga
    19081 gctggtttct ttaacgtcgg acttcctggt caggctttgg caggttggat tctcagtggt
    19141 tggtttgccc tgtcgcatcc agatatgccc cgtcccttga tgattctagc aaccatcgtg
    19201 attgccttga ttgctggtgg gattgtcgga gcgattccag gtattcttag ggcctatcta
    19261 gggacgtcag aggttattgt aaccatcatg atgaactaca ttgtcttgta tgtagggaat
    19321 gcctttatcc atgctttccc taaagacttc atgcaaagta cagattcgac cattcgtgtt
    19381 ggggctaatg caacctatca gacaccttgg ttggctgagt tgactggtaa ctcacggatg
    19441 aatattggta ttttctttgc catcattgcc gttgcagtta tttggttcat gctcaagaaa
    19501 acaactcttg gttttgaaat ccgtgcagtt ggtcttaatc cacatgcttc agaatatgct
    19561 ggtatttctg ccaagcggac tattatccta tctatgatta tttcaggtgc cttggcaggt
    19621 cttggtggag ctgttgaagg tttgggaacc ttccagaacg tctatgttca aggttcgtca
    19681 ttagctatcg gatttaacgg aatggcggtt agtttgcttg cggccaactc accaattggt
    19741 atactctttg cagccttcct atttggcgtt ctccaagttg gggctcctgg tatgaatgcg
    19801 gcgcaggtac catctgagct tgtcagcatt gtaacagcgt ctattatctt ctttgtcagt
    19861 gttcattacc ttatcgaacg ctttgtcaaa ccgaaaaaac aagttaaagg aggtaagtaa
    19921 agatgtctat tataaccttg ctccccctct tggtgtcttc tatgctgatt tactcagcac
    19981 ctctcatctt tacaagtatc ggtggtgttt tctctgaacg tggtggtgtg gtaaacgttg
    20041 gccttgaagg aattatggtt atgggtgcct tttctggagt tgtctttaac cttgaatttg
    20101 cagaacaatt tggagcagca actccatggc tatccttgct tgtagcagga ttggttggta
    20161 gtgttttctc tatcatccac gcagcagcga cggttcattt ccgtgcagac catgttgtca
    20221 gcggtacggt attgaacttg atggcgcctg ccttggctgt tttcttagtt aaagttcttt
    20281 ataacaaagg acaaaccgac aacctaagtc aaacttttgg acgctttgat ttcccagtct
    20341 tggcaaatat cccagtgatt ggtgatatct tcttcaagtc aactagtcta cttggttatc
    20401 tggcgattgc cttctcattc cttgcttggt ttattctctt taagactcaa tttggtcttc
    20461 gtctccgctc tgtcggtgaa catcctcaag cagcggatac cttgggaatc aacgtctaca
    20521 agatgagata tttagggatt attatttcag gttttctagg tggaattggc ggagcgattt
    20581 atgcgcaatc aatctcagtt aacttctcag tgacaactat tgttggacct ggatttatcg
    20641 cccttgctgc gatgatcttt gggaaatgga atccaatcgg tgctatgctt tctagtctct
    20701 tctttggact ttcacaaagt ttggctgtta tcggttctca attgccgttc ctacaaggag
    20761 tgccagcggt ttatcttcaa attgcacctt atgttttgac aattcttgtc ttagcagcct
    20821 tctttggaaa agcagtcgca ccaaaagcag atggtatcaa ctacatcaaa tcaaaataag
    20881 catacaaaaa aacgtcagtc caatgactga actgcccccc aaaagttaga cagaaaaaat
    20941 ctaacttttg gggtgttttt attatgaaat taagttatga tgataaagtt cagatctatg
    21001 aactaagaaa gcaaggatat agcttagaga agctttcaaa taaatttggg ataaacaatt
    21061 ctaatcttag gtacatgatt aaattgattg atcgttacgg aatagagttc ggcaaaaaag
    21121 gaaaaaatcg ttactattct cctgatttaa aacaagaaat gattcataaa gtctgacatg
    21181 aaggctggac taaagataga gtttctcttg aatacggtct cccaagtcgt acgatacttc
    21241 ttaactggct agcacaatac aggaaaaacg ggtatactat tgttgagaaa ccaagaggga
    21301 gagtacctaa aatgggacgt aatccaaaaa cgagacctga agagaggaca gaattagaac
    21361 gtcttcaagc agaaaatgag tacctgagag cggagaatgc catcctaaaa aagttaagag
    21421 aactccgatt gaaggaggaa aaagagaaag aagaaagaca gaaattgttc aagaattaat
    21481 gactgagttt tcgttagatc ttcttctaaa agccattaaa ctagctcgtt cgacctacta
    21541 ctatcacttg aaacagctag acaaaacaga taaggaccaa gagcttaaag ctgaaattca
    21601 atccatcttt atcgaacaca agggaaatta tgcttatcgt cggatttatt tagaactaag
    21661 aaatcgtggt tatctggtaa atcataaaag agttcaacac ttgatgaagt actcaattta
    21721 caagctaaaa cgcgacagaa acgaaaatat tcttctcata aaggagacgt tggcaagaaa
    21781 gcagagaatc tcattcaagg ccaatttgaa ggctctaaaa caatggaaca gtgctacaca
    21841 gatgtgactg aatttgccat tccagtaagt actcaaaagc tttacttatc accagtttta
    21901 gatggcttta atagcgaaat tatcgcctat aatctttcaa cttcacccaa cttagaacaa
    21961 gtacaaacaa tgttggaaca ggcattcaca gagaagcact acgagaatac gattctccat
    22021 agtgaccaag gctggcaata tcaacacgat tcttatcatc ggttcctaga gagtaaggga
    22081 attcaagcat ctatgtcacg caagggaaac agccaagaca acggtatgat ggagtccttc
    22141 tttgggattc tgaaatccga aatgttttat ggctatgaga aaacatttaa atcacttaac
    22201 caattggaac aagccattat agactatatt gattactaca acaacaaacg aattaaggta
    22261 aaactaaaag gacttagccc tgtgcaatac agaactaaat cctttggata aattatttgt
    22321 ctaactgttt gggggcagta cagaactggc gttttatttt ttaggatctt gatgggttag
    22381 gttcaatccc caagggacca aattttcagt tttatttttg atacgagcta tattgtcctt
    22441 atgacgaatg ataatcaaac tagcaagtgc taagataata gcgatgaaga gagagtcata
    22501 gttactcagg ataaaaccaa aaagtggaaa gagcagaacc ccgataacag ccgcgatcga
    22561 tgctgtgaca ctagacagtg aaatcatact gccaagatag agagctccaa agaagataat
    22621 cgcaaggtag agacagaaga taggcgcaaa tccgaaaatc actccagcac tggttgcgac
    22681 agccttacca cctttaaatc ctgcaaagat agggaaggta tggccgataa cagccaaaag
    22741 tccaaagatg agaggagaaa cgccttgtag atgaaaaata atcggaagca gcgttgctag
    22801 ggttcctttg aaaaagtcaa tcacaaaggt tgccatacca gctttcttac ctaaaatgcg
    22861 gaaggtgttg gtcgttccag tgttaccaga accatgctcg cgtagattga tttgaaagaa
    22921 tacttgtcca atccagagac cagatggaat cgaacccagc agataggcta ggattaataa
    22981 aactattgta atcatactcc tattatatca tgaattggga aagaaaagca gagaatctct
    23041 gctgaaattg tcacatcgga gaaagaaaaa ttttgcaaaa tccttggaaa acctgtagaa
    23101 tagtaaagat gaacgaatag gaggttcctt gtgtcaaaaa aggaaatcaa tattaacaat
    23161 tataatgatg atgctattca ggtgctagaa gggttggatg cggtccgaaa acgtccaggg
    23221 atgtatattg gatcgaccga tggcgctggt cttcatcacc tagtttggga aatcgttgat
    23281 aatgcagtcg atgaagcctt gtctgggttt ggtgatcgta ttgatgtaac tatcaataaa
    23341 gacggtagtc taacggttca agaccatgga cgtgggatgc cgacaggtat gcacgctatg
    23401 ggaattccaa ctgttgaggt tatctttacc attcttcatg ccggagggaa attcggtcaa
    23461 ggtggctata agacatcagg tggacttcac ggagtgggtt cttccgttgt taacgccctt
    23521 tctagctggt tggaagttga aattacccgt gatggcgcag tttacaagca acgttttgaa
    23581 aatggtggaa aacctgtcac gactttgaag aaaatcggta cagcactcaa gtctaaaaca
    23641 ggcaccaaag ttacttttat gcctgacgcg actatctttt ctacgacaga tttcaagtac
    23701 aataccattt cagagcgcct taatgaatca gcctttctct tgaaaaatgt gaccttgtct
    23761 ttaacggaca agcgaacaga tgaagcgatt gagttccact atgagaatgg agtacaagat
    23821 tttgtttctt atctcaacga agataaggaa atcttgacgc cagttcttta ctttgaaggg
    23881 gaagacaatg gttttcaagt ggaagtagcc ctccagtaca atgacggatt ctcagataac
    23941 attctatcct ttgtcaataa cgttcgcacc aaggacggtg gaacgcacga gacaggactc
    24001 aagtctgcca ttaccaaggt catgaatgac tatgcacgta aaacaggtct tctcaaggaa
    24061 aaagataaaa accttgaagg ttcagactat cgtgagggac tagcggccgt tctttctatc
    24121 ttagttcctg aagaacactt gcagtttgaa ggacagacca aggataaact aggaagcccc
    24181 ctagctcgcc cagttgtgga tggaatagtg gctgataagt tgaccttttt ccttatggaa
    24241 aatggggaat tagcttctaa cctcatccgc aaggctatca aggcccgtga tgctcgtgaa
    24301 gcagcacgta aggcgcgtga tgagagccga aatgggaaga aaaacaagaa agataagggc
    24361 ttgttgtctg ggaaattgac cccagcccaa tctaagaatc ctgctaagaa tgaactctat
    24421 ctagttgagg gggactctgc cggtggttct gccaaacaag gtcgtgaccg caagttccag
    24481 gctattctac ctcttcgtgg taaggttatc aatacagcca aggccaagat ggcggatatc
    24541 ctcaaaaatg aagagatcaa taccatgatt tataccattg gtgcgggtgt tggagcagac
    24601 ttctctattg aagatgccaa ctatgataag atcattatca tgaccgatgc ggataccgac
    24661 ggtgcccata tccagacctt gctcttgaca tttttctacc gttacatgcg tccgctagtc
    24721 gaggcaggtc atgtctatat tgccctccca cctctttaca agatgtccaa aggtaaaggc
    24781 aagaaagaag aagtggccta cgcttggacg gacggagaac tagaagaact ccgtaaacag
    24841 ttcggtaaag gcgctaccct ccaacgatac aaaggacttg gtgagatgaa tgcggaccag
    24901 ctctgggaaa caaccatgaa cccagaaaca cgtaccctca tccgtgtcac aattgaagat
    24961 ttagcgcgcg ccgaacgccg cgtcaatgtt ctcatgggag ataaggtaga accacgccgt
    25021 aaatggattg aagataatgt caagtttacg ctagaagaag cgacagtgtt ttaatgaaag
    25081 aaaataatat gttagtaaac tggaaattag agtcggatgt gaatgactat gtgaaaaagc
    25141 agtttgaaaa tcttggtttg aaaaaattgc aggattatta atgaagaatc tgctatgagt
    25201 gcttatctta aagaggcgct taagggagcg gttaagacac aaactaagac taattttgga
    25261 aaaccagatt ttcatattga aaaatacaaa caggatagtt tggttactta tcgtaactga
    25321 acacgggacg aattcccgta aaatatcttt tactgcataa aaacctactc tacattcttt
    25381 gaaaggagtt gaacacgccc tagatactgt gtgaaaaaga taaattctct tgtgagtttg
    25441 cttacttcaa gaattttcta ttttcacttg gtattttatg ggctttgtat cttatgtcta
    25501 acattcaaaa catgtccctg gaggacatca tgggagagcg ctttggtcgc tactccaagt
    25561 acattattca agaccgggct ttgccagata ttcgtgatgg gttgaagccg gttcaacgcc
    25621 gtattcttta ttctatgaat aaggatagca atacttttga caagagctac cgtaagtcgg
    25681 ccaagtcagt cgggaacatc atggggaatt tccacccaca cggggattct tctatctatg
    25741 atgccatggt tcgtatgtca cagaactgga aaaatcgtga gattctagtt gaaatgcacg
    25801 gtaataacgg ttctatggac ggagatcctc ctgcggctat gcgttatact gaggcacgtt
    25861 tgtctgaaat tgcaggctac cttcttcagg atatcgagaa aaagacagtt ccttttgcat
    25921 ggaactttga cgatacggag aaagaaccaa cggtcttgcc agcagccttt ccaaacctct
    25981 tggtcaatgg ttcgactggg atttcggctg gttatgccac agacattcct ccccataatt
    26041 tagctgaggt catagatgct gcagtttaca tgattgacca cccaactgca aagattgata
    26101 aactcatgga attcttacct ggaccagact tccctacagg ggctattatt cagggtcgtg
    26161 atgaaatcaa gaaagcctat gagactggga aagggcgcgt ggttgttcgt tccaagactg
    26221 aaattgaaaa gctaaaaggt ggtaaggaac aaatcgttat tactgagatt ccttatgaaa
    26281 tcaataaggc caatctagtc aagaaaatcg atgatgttcg tgttaataac aaggtagctg
    26341 ggattgctga ggttcgtgat gagtctgacc gtgatggtct tcgtatcgct attgaactta
    26401 agaaagacgc taatactgag cttgttctca actacttatt taagtacacc gacctacaaa
    26461 tcaactacaa ctttaatatg gtggcgattg acaatttcac acctcgtcag gttgggattg
    26521 ttccaatcct gtctagctac atcgctcacc gtcgagaagt gattttggcg cgttcacgct
    26581 ttgacaaaga aaaggctgag aaacgtctcc gtatcgtcga aggtttgatt cgtgtgattt
    26641 cgattttgga tgaagtcatt gctcttatcc gtgcttctga gaataaggcg gacgccaagg
    26701 aaaacctcaa agttagctat gattttacgg aagaacaggc tgaggctatc gtaactttgc
    26761 aactgtaccg tttgaccaat accgatgtgg ttgtcttgca ggaagaagaa gcagagcttc
    26821 gtgagaagat tgctatgctg gcggctatta tcggtgatga aaggactatg tacaatctca
    26881 tgaagaaaga acttcgtgag gtcaagaaga actttgcaac tcctcgtttg agttctttag
    26941 aagacactgc gaaagcaatt gagattgata cagctagtct tatcgctgag gaagatacct
    27001 acgtcagcgt gaccaaggca ggttacatca agcgtaccag tccacgttcc tttgcggctt
    27061 ccaccttgga agaaattggc aagcgtgatg atgaccgttt gatttttgtt caatctgcca
    27121 agacaaccca gcacctcttg atgttcacaa gtcttggaaa tgtcatctac agaccaatcc
    27181 atgagttggc agatattcgt tggaaggaca tcggagagca tctgagccaa accatcacaa
    27241 actttgaaac gaatgaagca atcctttatg tggaagtact ggatcagttt gacgatgcga
    27301 caacctactt tgcagcgact cgccttggtc aaatcaaacg ggtagagcga aaagaattca
    27361 ctccatggcg gacctataga tctaagtctg tcaagtatgc taagctcaaa gacgatacag
    27421 atcagattgt agcagtggct ccgattaaac tagatgatgt tgtcttggtt agtcaaaatg
    27481 gttatgccct gcgtttcaat atcgaagagg ttccggttgt cggtgctaag gcagcaggtg
    27541 tcaaggctat gaatttgaaa gaagatgatg tcctccaatc tggctttatc tgtaatactt
    27601 cgtccttcta cctcttgacc cagcgtggaa gcttgaaacg tgtttccatt gaggaaattc
    27661 tagcaaccag ccgtgccaaa cgaggattac aagtcttgcg tgagttgaaa aacaaaccgc
    27721 atcgtgtctt cttggcagga gcagttgcag agcaaggatt tgttggcgat ttcttcagta
    27781 cggaagtgga tgtgaacgac caaactctgc ttgtccaatc caataaagga acaatctatg
    27841 aaagccgatt gcaagacttg aacttgtcag aacgcactag caatggaagc ttcatttctg
    27901 acacgatttc agatgaagaa gtttttgacg cttatcttca ggaagtagtt actgaagata
    27961 aataagtgaa atgaagaatc agttctaaga tggtttttat tggaggaatt ttctgaaaat
    28021 tacaaaatat acttgctatt ttagaaaaat agtgtagaat ataagaaata ggtttttaga
    28081 ataaggagtg gaatatgaca gtaacgattg attgggaaaa cctcggtttt tcctatatga
    28141 aattacctta tcgctatctt gctcatttca aaaatggaca atgggatcaa ggagagctta
    28201 cagaggatgc aactttgcat atttcagagt cttctccaag tcttcactat ggacaacaag
    28261 catttgaagg tttgaaagct tatcgtacta aggatggcag tgttcaactg ttccgtcctg
    28321 atgaaaatgc taaacgcctg caacgtacat gtgaccgtct cttgatgcca caagttccga
    28381 cagacatgtt tgtagaagct tgtaaagcag ttgtccgtgc gaatgaagaa tacgtaccac
    28441 catacggaat aggtggaacc ttatatcttc gccctctttt gattggtgtc ggagatatta
    28501 tcggggtaaa accggcagaa gagtacattt tcaccatctt tgctatgcca gttggaaatt
    28561 actttaaagg tggtttggtc ccaaccaact tcttgattca ggatgagtac gaccgtgcag
    28621 caccaaatgg tacaggtgcg gctaaggttg gtggaaacta tgctgcaagt ctcttaccag
    28681 gaaaaatggc caagtcacgc catttctcag atgttatcta tctggaccca tcaactcata
    28741 caaagattga agaagtcgga tcagctaatt tctttggaat tacagctgat aatgaatttg
    28801 taacaccatt gagtccatct atcttgccat ctattaccaa gtattccttg ctttatttgg
    28861 cagaacatcg cttgggatta actcctattg agggtgatgt tccaattgat aatcttgacc
    28921 gttttgtaga ggcaggtgcc tgtggtacag cagcggttat ttctccaatt ggaggtattc
    28981 aacatggtga tgatttccat gtattctata gtgaaacaga agtaggtcct gtgacgcgta
    29041 aattatataa tgaattgacg ggtattcagt ttggcgatat tgaagcgcca gaaggttgga
    29101 ttgtaaaagt agattaaaat aaaccaaagg agatttttta tgaaatagaa aaagtggctc
    29161 ttaacagcag gagtggtcct gagcacgtta gctattttag tggcttgtgg aaaaactgat
    29221 aaagaagcag atgcaccgac aacattttct tatgtctatg cagtagatcc agcatcattg
    29281 ggctacagta taacgactcg aacatcgacg acagacgtta ttggaaatgt tattgatggt
    29341 ttgatggaaa atgataaata cggcaatgtt gctccttctc aaaaagacta tgatttgaat
    29401 agtacaggat gggctccaag ctatcaagat ccagcgtctt acttgaatat tatggatcca
    29461 aaatctggtt ctgccatgaa acaccttggt attacgaaag gaaaagataa ggatgttgta
    29521 gctaaacctg gtttggataa atataagaaa ttgttagaag atgctgtttc tgagaccact
    29581 gacctagaga agagatatga aaaatatgcc aaagctcaag cttggtcgac agatagttca
    29641 ttattgatgc caacagcttc atctggtggt tctccaattg taagtaacgt agtaccattc
    29701 tcaaaaccat actcacaagt tggtattaag ggggaaccat atatctttaa aggaatgaaa
    29761 ttgcaaaaag atattgttac aacaaaagaa tataacgagg tttttaaaaa atggcaaaaa
    29821 gaaaaattgg aatccaatag caaataccaa aaagaactag aaaaacacat taaataagga
    29881 atggtattga tcttgataaa atttttaaaa tactgtcatt ttgaatataa aggagtttga
    29941 tatggagtgg attagattaa taggaatagc aatcattgtt gtgggtttta ttttaaaatt
    30001 tgatacaatt gcaacagtag tcttagctgg tttggttaca gctttagttt caggtgtttc
    30061 tctcgttgaa tttttggaga ttttgggaaa agaatttagc aatcagcgag tgctcacgat
    30121 ttttatggtt accttgcctc ttgtggggct gtcagaaacc tttggactca agcaacgatc
    30181 aatcgatttg attcgaaaga ttaaaggtct gacagttgga aacttctata cagtttattt
    30241 ctttattcga gagttagctg gtttcttttc aattcgtcta ggaggacacc ctcagtttgt
    30301 cagacctttg gttcaaccta tgggagaagc agctgcagag tctcaattag gtagaaagtt
    30361 aacagaggtt gaagatgaga caataaaagc gcgtgcggct gcgaatgaaa attttggaaa
    30421 tttctttgct caaaatacgt ttgtaggtgc tgggggagtc ctcttgatag ggggaacatt
    30481 agatcagtta ggctatgaaa gcaattatgc agggattgct tctacatcta ttattgttgc
    30541 tgttataaca cttattgtag tggggattta caattattta tttgataaaa aattgatatc
    30601 aaaaaagact aggggaggag aacaaaaatg acagagttgg caaagcaact attagagttg
    30661 acctatattg tgattggttg tcaatttctc catacagcct attgtagtta taaagataaa
    30721 acaaacccag ttcgacttgg gacatctgca ttttggactc tattgtctat tacgtttata
    30781 ggtggttcct atatgccaaa tatgagtatt ggtattattg taatcctatt atcgctgtta
    30841 acattgttta agcaagtccg tatcggaacc ttgccatcct tagatgaaat gaaagccaat
    30901 attgaatcta acaggttgaa aaataaaatt tttattccag ttatgctgat ggcaatactt
    30961 gcgttggtct tagcgcaaat gattccagaa tttagcaaga tttcgattag ccttgccgcc
    31021 ttgtttgcta caatttctgt tcttgtgatt accaatagtc accctaagag tctgttatca
    31081 gaaaataatc gaatgactca gcaagtttca acaagtggga ttgttcctca attattaggg
    31141 gctttggggg ctatttttac tgtagcaggt gttggtgatg ttatctctca tctgattagc
    31201 ggtattgttc cttcagatag tcgctttata ggagttttgg cctatgttct tggaatggtt
    31261 ctattcacaa tgattatggg aaatgctttt gcagcattca ccgttattac agcaggtgtt
    31321 ggagttccct ttgtatttgc tctgggagct aatccaattg tggctggtgc tcttgccatg
    31381 acagcaggtt attgtgggac cttattgacc ccaatggctg ctaattttaa cgctctacca
    31441 gcagcattga tggatatgaa agatcagaat ggcgttataa aggctcaagc aggtgttgct
    31501 ctagtaatga ttgttattca catattctta atgtactttc tcgcatttta gtaaaggaaa
    31561 ataaaatgaa aatattggtt acaggtttta atccttttgg aggtgaaaag attaatccag
    31621 ctttggaggc tgtaaaatta ttaccatctg agattaatgg ggctgaagtt cgctgggtag
    31681 aaattccaac ggttttttat aagtcgtcag aagttttaga ggcagaaata ttacgatatc
    31741 aaccagatgc tgtactttgt attggacaag caggcggcag gaccggctta acacctgaac
    31801 gagtggctat taatcaagat gatgctcgca tacctgataa cgaaggcaat caaccaattg
    31861 atacaccgat tcgtattgat ggagcatcgg cctattttag tagtttacct atcaaagcga
    31921 tggtacaagc tatcaaaaaa cagggacttc cggcagttgt atccaatagt gcaggaacct
    31981 ttgtttgcaa tcatttgatg taccaagctc tttatttagt ggataaaaaa ttccctaata
    32041 tgagagcagg tttcatgcat attccatata tgatggaaca agtagtaaat aagccgaata
    32101 ctgcaggaat gagtctatgt gatattgtaa gaggcataga ggtagctatt gaagctattg
    32161 tagactacaa agataaggat ttgcagttag taggcggtga gactcactga taacctaaaa
    32221 aggatagtca attatgcttg tttactaact attaactatg ctaaatcgat tgaggttgtt
    32281 tacataaaac tctatatcag agaagcctga tatagagttt tttcttgcta gttttaggat
    32341 tttcttgtaa aatagaaaaa gtgaagagag gtatgaaatg agcaagaaag ataaaaaaat
    32401 cgaaattcaa gtagcggatg ccaaagttaa tgttggtaaa gacagttttg aaggttatac
    32461 attgactatc ggtaaaaaag ttatcggaga aattgccgaa ttagacggac aatttgccat
    32521 tataaagaat gggaatgtcg atagttttta taaaaaattg gaaaaagctg tggaaatttt
    32581 gattgaaaat tataatttag caaaataagt cttgtttttt tgaaattttc atgatataat
    32641 agtccatgtt gattgtagga gagatagcga agaggctaaa cgcggcggac tgtaaatccg
    32701 ctccttcggg ttcgggggtt cgaatccctc tctctccatt tcattaatgg ggtatagcca
    32761 agcggtaagg caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt
    32821 tcttaggtaa taatcaagat aaaaagcaaa atatcttagg gtattttatt tttataattg
    32881 aaagacgtga atgatatgaa catgtccttg cgggtgctta ggaaaaaaat tataagtatg
    32941 tcaagtttaa gaaaaacttg attgttggag gattttttag atgaatgaat ttgaagattt
    33001 gctaaatagc gttagtcaag ttgagactgg tgatgttgtt agtgctgaag tattgacagt
    33061 tgatgcgact caagctaacg ttgcaatctc tggaactggt gttgaaggtg tcttgactct
    33121 tcgcgaattg acaaacgatc gtgatgcaga tatcaatgac tttgttaaag taggagaagt
    33181 attggatgtt cttgtacttc gtcaagtagt tggtaaagat actgatacag ttacatacct
    33241 tgtatctaaa aaacgccttg aagctcgcaa agcatgggac aaacttgttg gtcgcgaaga
    33301 agaagttgtt actgttaaag gaacgcgtgc cgttaaaggt ggactttcag tagaatttga
    33361 aggtgttcgt ggatttatcc cagcttcaat gttggatact cgtttcgtac gtaacgctga
    33421 gcgttttgta ggtcaagaat ttgatactaa aatcaaagaa gttaacgcta aagaaaaccg
    33481 cttcatcctt tcacgtcgtg aagttgttga agcagctact gcagcagctc gcgctgaagt
    33541 attcggtaaa ttggctgttg gtgatgttgt aactggtaaa gttgctcgta tcacaagctt
    33601 cggcgctttc gtcgaccttg gtggtgttga cggattagtt cacttgactg aattgtcaca
    33661 tgaacgtaat gtatcaccaa aatcagttgt aactgttggt gaagaaattg aagtgaaaat
    33721 ccttgatctt aacgaagaag aaggacgtgt atcactttca cttaaagcaa cagtaccagg
    33781 accatgggat ggcgttgagc aaaaattggc taaaggtgat gtagtagaag gaacagttaa
    33841 acgtttgact gacttcggtg catttgttga agtattgcca ggtatcgatg gacttgttca
    33901 cgtatcacaa atttcacaca aacggattga aaatccaaaa gaagctctta aagttggtca
    33961 agaagttcaa gttaaagttc ttgaagttaa cgcagatgca gaacgcgtgt cactttctat
    34021 taaagctctt gaagaacgtc cagcccaaga agaaggacaa aaagaagaaa aacgtgctgc
    34081 tcgtccacgt cgtccaagac gtcaagaaaa gcgtgatttc gaacttccag aaacacaaac
    34141 aggattttca atggctgatt tgtttggtga tatcgaactt taatcaaatt gaaaattcac
    34201 aaaatccttt gtttactaaa caagggattt ttctggctct ttgtcaactg tagtgggttg
    34261 aagaaaagct aagctcgaga aaggacaaat tttgtccttt cttttttgat attcagagcg
    34321 ataaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata
    34381 agtttgatga gattattggt cgcttccagt ttggtgttag aatagtgtag ttgaagggtg
    34441 ttgacaagct tttctttatc tttgaggaag gttttaaaga cagtctgaaa aataggatga
    34501 acctgcttaa gattgtcctc aataagtccg aaaaatttct ccggttcctt attctgaaag
    34561 tgaaacagca agagttgata gagctgatag tggtgtttca agtcttccga ataactcaaa
    34621 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcgc
    34681 ttatcactca gtttacgact atcctgttgt atgagcttcc agtagcgctt gatagccttg
    34741 tattcatggg attttcgatg aaactgattc atgatttgga catgcacacg actcatagca
    34801 cggctaagat gttgtacaat atgaaagcga tcaagaacga ttttagcatt cgggagtgaa
    34861 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagt tgtctagcta
    34921 agtcatagta agggctaaac atatccatag taatgatttt gacgcgacat cggacggctc
    34981 tatcatattt aagaaagtga tctcggatga tagcttgtgt tctaccttca agaacagtga
    35041 tgatatcgag cttgtcaaaa tcttgcgcaa taaagctcat cttcatctcc cgattgaaac
    35101 agtcactccc cggactgttt caacgtccca ggacataatc tcaggaagac gcgaaaaatc
    35161 atgctcaaag tgagaatcat tgagctttcg aatgacagtt gaagttgaaa tggccagctg
    35221 atgagcaata tcggtcatag aaatcttctc aatcaacttt tgcgcaattt tttggtcgat
    35281 aatacgagga atttggtgat ttttcttgac gatagaagtt tcagcgacca tcatttttga
    35341 acagtgatag cacttgaaac gacgctttct aagtagaatt ctagtaggca taccagttgt
    35401 ctcaaggtaa ggaatcttag acggtttttg aaagtcatat ttcttcattt gacttccgca
    35461 ctcagggcaa gatggggcgt cgtagtccag tttggcgatg atttctttgt gtgaatccct
    35521 attgacgaca tctataattt ggatatttgt gtctttgata tcgagtagtt ttgtgataaa
    35581 atgtaattgt cccatatgaa tctttctaat gagttgtttg gtcgcttttc attataggtc
    35641 atatgggact ttttttctac acaaaaatag gctccataat atctatagtg gatttaccca
    35701 ctacaaatat tatagagcca aaaatccttt gtttactaaa caagggattt ttcttttgtc
    35761 tctgctcctt ttttgatata atagttctat gttaaaatca gaaaaacaat cacgttatca
    35821 aatgttaaat gaagaattgt ccttcctatt ggaaggcgaa accaatgttt tggctaatct
    35881 ttccaacgcc agtgctctca taaaatcacg ttttcctaat accgtatttg caggctttta
    35941 tttgttcgat ggaaaggaat tggttttagg ccccttccaa ggaggtgttt cctgcatccg
    36001 tattgcacta ggcaagggtg tttgtggtga ggcagctcac tttcaggaaa ctgttattgt
    36061 tggagatgtg acgacctatc tcaactatat ttcttgtgat agtctagcta aaagtgaaat
    36121 tgtggtgccg atgatgaaga atggtcagtt acttggagtt ctggatctgg attcttcaga
    36181 gattgaggat tacgatgcta tggatcgaga ttatttggaa caatttgtcg ctattttgct
    36241 tgaaaagaca gcatgggact ttacgatgtt tgaggaaaaa tcttaatgta tcaagcactt
    36301 tatcgaaaat atagaagtca aaacttctcc cagttagttg gtcaagaagt tgtggctaag
    36361 actcttaaac aagcggtgga gcaagagaaa ataagtcacg cttatctttt ttctggtcct
    36421 cgtggaacgg gaaaaaccag tgttgctaaa atctttgcca aggctatgaa ctgtcccaat
    36481 caagtgggtg gcgaaccttg caataactgc tatatttgtc aagcagtgac ggacggtagt
    36541 ttagaagatg tcattgaaat ggatgcagct tctaataatg gggtagatga aattcgcgaa
    36601 attcgtgata aatctaccta tgcgcctagc cttgctcgtt ataaggttta tatcatagat
    36661 gaggttcaca tgctgtctac aggggctttt aatgccctcc taaagacgct ggaagaacca
    36721 acacagaatg tagtctttat tttggccact actgaattgc acaagattcc tgctactatt
    36781 ctatcccgtg tgcaacgttt tgagtttaaa tcaattaaga cacaggatat taaggaacat
    36841 attcactata tcttagaaaa agaaaatatc agttctgaac cagaggctgt ggaaatcatt
    36901 gccagacggg cggaaggtgg aatgcgggac gccttgtcta ttttggatca agccctgagt
    36961 ttgacacagg gaaatgagct gacgactgct atctctgaag aaattactgg caccattagc
    37021 ctatcagcct tggatgatta tgtggcggcc ttgtctcaac aggatgttcc caaagctttg
    37081 tcttgcttga atcttctttt tgacaatggt aagagcatga ctcgttttgt gaccgatctt
    37141 ttgcactatt taagagactt gttaattgtt caaacagggg gagcaaatac tcatcatagt
    37201 tcagtctttg tagaaaattt ggcacttcct caaaaaaatc tgtttgaaat gattcgctta
    37261 gcaacagtga gtttagcaga tattaagtct agtttgcaac ccaagattta tgctgaaatg
    37321 atgaccgtcc gtttggcgga aatcaagtcc gaaccagctc tatcaggagc ggttgaaaat
    37381 gaaattgcta cgctgagaca ggaagttgcc cgtctcaaac aagagctttc taatgtaggt
    37441 gcggttccta aacaagttgc accagctcct agtcgaccag ctacgggcaa aacagtctat
    37501 cgtgtcgatc gcaataaagt gcaatctatc ttacaagagg ccgtcgaaaa tcctgattta
    37561 gcacgtcaaa atttaattcg tttgcagaat gcctggggag aggtaattga aagtctaggt
    37621 gggccggaca aggctctgct agttggttct caaccggttg ctgccaatga acaccatgct
    37681 attcttgctt ttgagtctaa cttcaatgct ggtcaaacta tgaaacgaga caatctcaat
    37741 accatgtttg gtaatatcct cagtcaggcg gcaggttttt cacctgagat tttagctatt
    37801 tccatggagg aatggaaaga agttcgcgca gccttttcag ccaaagccaa atcttctcaa
    37861 actgaaaaag aagtagaaga aagcctgatt ccagaaggat ttgaattttt ggctgataaa
    37921 gtgaaggtag aggaagacta aagaaagatt tcatgataca ataagtttat gaataaacaa
    37981 caatttatta ttatggcgct atttacagct gctgagacct attttttcaa tgaagcctgg
    38041 atgactggcc gctatattat ggcagccttt tgggcaattt tactctttag aaatttccga
    38101 gtcagttatg tgatgggtaa aatcgttgat gtcatcgatc agcattttaa taggaaagac
    38161 tagccctcag cttccagaca aaatcaaagc cttttaggct tttttttgtt atactagaaa
    38221 agtatattta tagaattttt gctctatttc tggggaaatc agacgttttt ctagtaagta
    38281 ctgtaaaagt tttgaaaaag aaaggaacta tcatgtcagt aatagagatc aaagatcttc
    38341 acgttgagat tgaaggaaaa gaaattttaa aaggggttaa cctgaccctg aaaacaggag
    38401 aaattgccgc tatcatggga ccaaatggta caggtaaatc gactctttct gccgctatca
    38461 tgggaaatcc aaactatgaa gtaactaaag gtgaagtttt gtttgatggc gtaaacatcc
    38521 ttgagttgga agtggatgag cgtgcgcgta tgggactttt ccttgctatg caatacccat
    38581 cagaaatccc tggaattacc aatgctgagt ttcttcgtgc cgctatgaat gcgggtaaag
    38641 aagatgatga gaagatttca gttcgtgagt ttattactaa gctagatgaa aaaatggaat
    38701 tgctcaacat gaaagaagaa atggcagagc gttacctcaa cgaaggcttc tctggtggtg
    38761 agaaaaaacg caatgaaatt cttcaacttt tgatgttgga gccaacattt gctcttttgg
    38821 acgagattga ctcaggtctt gatattgacg ctcttaaagt tgtgtctaaa ggtgtcaatg
    38881 ccatgcgtgg tgaaggtttt ggtgctatga tcatcactca ctaccaacgt cttttgaact
    38941 atatcacacc tgatgtggta cacgtgatga tggaaggtcg tgttgtcctt tctggtggtc
    39001 cagaattggc tgcgcgtttg gaacgtgaag gatacgcaaa attagctgaa gaacttggct
    39061 acgactacaa ggaagaattg taattccctc gtatctttta ggagaagtaa atgactagag
    39121 aaaatattaa acttttttca gaaatgcacg ctgaaccaag ctggttggct gatctccgtc
    39181 aaaaagcttt tgacaagatt gagactttgg aattaccagt tattgagcgt gtcaaattcc
    39241 accgttggaa tctgggtgat ggaacgatta cagaaaatga gccatcagca aatgttccag
    39301 atttcacagc tttagatcat cacttgaagt tggtgcaagt aggaactcaa actgttttcg
    39361 aacaaactcc agttgagtta gctgaacagg gtgttgtctt cacagacttt cactcagctt
    39421 tagaagaaat tccagagctg atcgaagaat tcttcatgtc atctgttaag tatgatgatg
    39481 acaagttggc ggcttaccac acagcttact ttaacagtgg tgctgtactc tatattccag
    39541 ataacgtaga aatcacagag ccaattgaag gaattttcta ccaagatagc gatagcaatg
    39601 tgccgtttaa caagcatatt atgattatcg ctggtaaaaa ttctaagatt aattatctgg
    39661 agcgtttaga gtcacgcggt gaaggaagtg ccaaagcaac tgctaatatc acagtagaag
    39721 tgattgcacg ttcaggtgcg caagtcaagt ttgctgctat cgaccgacta ggtgaaaacg
    39781 tcactgccta cattagccgt cgtggtaaat taggcaacga tgcaagtatt gactgggcta
    39841 tcggtgtcat gaacgaagga aatgtcgttg ctgattttga tagtgacttg attggtaatg
    39901 gtagccatgc tgacctgaag gttgtagctc tttcaagtgg tcgtcaggta caagggattg
    39961 atactcgtgt aactaactat ggctgcaact ctatcggaaa tatcctacaa catggggtta
    40021 tccttgaaaa agcaactttg actttcaatg gtatcggcca catcatcaag ggtgctaagg
    40081 gagcagatgc ccaacaagag agccgtgttc tcatgctttc agaccaagcg cgttcagatg
    40141 ctaacccaat tcttttgatt gatgaaaatg atgtcactgc aggacacgca gcgtctatcg
    40201 gacaagttga tccagaagac atgtattacc tcatgagtcg tggattggat aaggcaactg
    40261 cagagcgttt ggttgttcgt ggtttccttg gatccgttat cgtggagatt ccagttaagg
    40321 aagttcgtga tgaaatgatt gcaactatcg aagagaaatt gtcaaaacgc taaggggaag
    40381 cctatgttag atgtagaagc tattcgcaag gattttccaa ttttagatca gattgtcaat
    40441 gatgaacctc tggtctatct ggacaatgct gcgacgacac aaaaaccact agtagttctg
    40501 aaagctatta acagctacta tgagcaggac aatgccaatg ttcaccgtgg tgtccatacc
    40561 ttagcggaac gagcgacagc ttcttatgaa gctgctcgtg aaaccattcg taagtttatt
    40621 aatgcaggct ctacaaagga agttctcttt accagaggaa cgacaaccag ccttaactgg
    40681 gtagcacgct ttgctgagga aattctcatt gagggagacc aggtcttgat ttcagtaatg
    40741 gaacaccatt ctaatatcat tccatggcag gaagcttgtc gaaagactgg agcagagctt
    40801 gtctatgtct atcttaaaga cggtgccttg gatatggagg atttgcgagc taaattgact
    40861 gataaggtta aatttgtttc cctagctcat gcctccaatg tacttggtgt ggtcaatccg
    40921 atcaaggaaa tcactcaatt agcccaccaa gttggggcaa tcatggtagt ggatggtgct
    40981 caatctacac ctcatatgaa gattgatgtc caggacttgg atctggactt tttcgccttt
    41041 tcgggtcaca agatggctgg tccgactggt atcggtgtcc tttacggcaa agaaaagtat
    41101 cttgagcaaa tgtctccagt agaatttggc ggcgagatga ttgattttgt ctacgagcaa
    41161 tctgctagtt ggaaggaatt gccttggaaa tttgaggctg gaacgccaaa tatggcagga
    41221 gctattggac ttgcgactgc agttgattat ctggaaaaga ttggtatgga tgccgttgaa
    41281 gctcatgaac aggaattgat tgcgtacgtc tatccaaaac tgcaggcaat tgagggattg
    41341 accatttacg gttctcagga tttggctcaa cgttcgggtg ttattgcctt taacctaggt
    41401 gatctccatc ctcacgatct tgcgacggtt ctggattatg aaggagtggc tgttcgtgct
    41461 ggtcaccatt gtgcgcaacc cttgcttcag tatttggaag tcccagcaac agctcgtgca
    41521 agtttttata tctacaatac caaggcagat tgcgacaaac tagtcgatgc cctacaaaag
    41581 acaaaggagt ttttcaatgg cactttctaa actagatagc ctttatatgg cagtggtagc
    41641 agaccattcg aaaaatccac atcaccaagg gaagttagaa gatgctgagc aaatcagtct
    41701 caacaatccg acttgtgggg atgtcatcaa cctctctgtc aagtttgatg cagaggaccg
    41761 tttggaagat attgcttttc taaattcagg atgcacgatt tcaactgctt ctgctagtat
    41821 gatgacagat gccgttttag gaaaaaccaa acaagaaatt ttagaactgg cgactatttt
    41881 ttctgaaatg gttcaagggc aaaaagatga gcgtcaaggc caacttggag acgcggcatt
    41941 cttgtcaggt gttgccaaat tccctcaaag aatcaagtgt gcaaccctag cttggaatgc
    42001 ccttaagaaa acaattgaaa atcaagaaaa acagtaagac aagtttcttt tgtcttatga
    42061 attattagaa atgaagaaag aaaggatact atggctgaag aaagagtaga accaaaacca
    42121 attgaccttg gtgaatataa atttggtttc catgacgatg tagagcctgt cttatcgaca
    42181 ggaaaaggac tcaacgaagg tgttattcgt gaattatctg ctgctaaggg tgagcctgag
    42241 tggatgttgg agttccgttt gaagtcttat gaaaccttca aaaaaatgcc catgcaaact
    42301 tggggagcag acttgtcaga gattgacttt gatgacttaa tctactacca aaaaccatct
    42361 gacaaaccag cccgttcttg ggatgatgta cctgaaaaga ttaaagaaac ctttgaacgt
    42421 atcgggattc cagaagctga acgtgcttat ttagcagggg cttctgccca gtacgagtca
    42481 gaagtggttt accacaacat gaaggaagag ttccaaaaat taggtattat ctttacagat
    42541 acagattccg cactcaagga atacccagac ttatttaaac aatactttgc gaagttggta
    42601 ccgccgacag ataacaagtt ggcagccctc aactcagcag tatggtcggg tggaactttt
    42661 atctacgtgc caaaaggtgt caaggtagat attccacttc aaacttattt ccgtatcaat
    42721 aacgaaaata taggtcagtt cgaacgtacc ttgattatcg ttgatgaggg agcaagcgtc
    42781 cactacgtag aaggatgtac agcaccaaca tattcaagca atagcttgca cgctgccatt
    42841 gtagaaattt ttgctttgga cggagcttat atgcgttata caactatcca aaactggtct
    42901 gataacgtct ataacttggt aacaaagcgt gctaaggctc aaaaggatgc cactgttgag
    42961 tggattgatg gaaacttggg tgccaaaacg actatgaaat atccatctgt ttaccttgat
    43021 ggagaaggag cgcgtggtac catgctctct atcgcctttg ctaatgcagg gcaacaccaa
    43081 gacacgggtg ctaagatgat tcacaatgct ccacatacca gctcgtctat tgtgtctaaa
    43141 tccatcgcta aaggtggagg aaaggttgac taccgtggac aagtcacctt taacaagaac
    43201 tctaagaaat ctgtttccca cattgaatgt gataccatta tcatggatga cttgtcagca
    43261 tcagatacta ttccatttaa tgaaattcac aactcgcaag tggctttgga acacgaagcc
    43321 aaagtatcta agatttcaga agagcaattg tattatctca tgagccgtgg attgtcagaa
    43381 tctgaggcaa ctgaaatgat tgtcatggga tttgtagaac cctttacaaa agaacttcca
    43441 atggaatacg cagttgagct gaaccgcttg attagctatg aaatggaggg atcagttgga
    43501 taaaatttga ttttatactc ttcgaaaatc tcttcaaacc acgtcagcat cgccttaccg
    43561 tatgtatggt tactgacttc gtcagtttca tctacaacct caaaacagtg ttttgagcaa
    43621 cctgcggcta gcttcctagt ttgttctttg attttgaaga tgaacttgta tcaaaaaatc
    43681 gcggtttaaa atcgcgattt tttataattt ctcgttaaca aagcggacaa actgattcca
    43741 ccaaactttt aagaagaagg ctttttcaat tttcttgtct gctaccattt cgaaactagg
    43801 gcgctctgtg gtgatgtaac cttgaccaat caagtccttg tcttcataag tcaaatggcc
    43861 aaccactgtt ccagcttcaa gtggtgctgg gattgctttg gaatcaggtg tgaattgaac
    43921 agattgggaa gattgattcc caacacgttc gattagatag atatcctctg gagccactgc
    43981 agttactgta tcttcttttc catcttgtac aggggctttg ctatcttgat aggcatcgcc
    44041 ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata taatccatta gggaagatgt
    44101 agctgtaaat cgagcgtaag gattattgtc ttgatgatct gcatttaaaa caactgtgat
    44161 gactctcata cctttttcga cagtagtacc aacaaaagac tctccagcct tatctgttgt
    44221 tcctgttttt agcccatcaa aaccaccacg gtaagcaggc ataccttcta acatgtagtt
    44281 ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt tttttggtga tttctaagac
    44341 ttgtgggtat tttttgatga ggttgcgagc aacgatagcg acatcataag cactaagctt
    44401 attttcctca tcttttttag aacctgggta aatgttatcc cctagagttt cattgttaag
    44461 acctgtcgta ttgacaacag tggcatcctg aattccccat tccaagagtt ttgcccgcat
    44521 catatcgacg aaatcttttt ctgagccagc aattttctca gctagggcaa tagcggcgct
    44581 gttggcacta gataccagag ttgcttcaag caactcttcg acagtataat tacgggcctc
    44641 cataggaata ttactggctt cagaatttgt cgtcaattga taaggataat cagaaatatc
    44701 tacaggagtg gagagggtaa tacttccgtt ttccaaagct tcatagacca gataaacagt
    44761 aatcaatttt gttatggaag caatttcgac aggttgcgtt gcatccttct catagagaat
    44821 tttaccagta tttgcctcaa cagcaatcgc atgtttagcg gcaatggtaa aatcttgagc
    44881 aacagcagta gaagcacccc ctaaaagaga gacagttaac aaagttaaaa atattttttt
    44941 catagtagtt ttattctatc ataaagaaaa aaaatattct tgctttaata attcatctgt
    45001 taagcttttt gaaaatatgg taaaataaag tgagggaggt aactcatgtt tcgtagaaat
    45061 aaattatttt tttggaccac agaaatttta ctcttaacca tcatctttta cctatggaga
    45121 cagatggggt ctttgattaa cccttttgtt agcgtgctta atacaattat gattccattt
    45181 ttattagggg gctttcttta ttatttgaca aaccctattg ttactttctt aaataaagtc
    45241 tgtaaactca atcgtttgct tggtatttta attaccttgt gtactttggt ctggggaatg
    45301 gtcataggtg ttgtctatct cttacctatt ttgattaatc agttatctag tttgattata
    45361 tctagtcaaa ctatttatag tcgagtacaa gacttaatca tagacttatc taattatcct
    45421 gcgctccaga atttggatgt agaagctaca attcagcagt taaacttatc ctatgttgat
    45481 attcttcaaa atatcctaaa tagcgtatca aatagtgtgg ggagcgtctt gtcagctctt
    45541 atcagtactg ttttgatttt gattatgact ccagtttttt tggtttattt cttattagat
    45601 ggacataaat tcttgcccat gcttgaaaga acgattctaa agagggatcg cttgcatatt
    45661 gcaggcttat taaagaattt aaatgcgacg attgctcgct atattagtgg agtttcgatt
    45721 gacgcaatca ttataggttg tttggcttat attggctata gtattattgg tttaaaatat
    45781 gctttagttt ttgccatttt ttctggtgta gccaatttaa ttccttatgt ggggccaagt
    45841 attggtttga ttcctatgat catcgcaaat atattcactg atccccatag actgctgatt
    45901 gcagtgattt atatgcttgt tgttcagcag gtagatggca atatcttata tcctcgaatt
    45961 gtaggaagtg ttatgaaggt tcatccaatc acgattttag ttttactttt gttgtcaagc
    46021 aatatctatg gtgtagttgg aatgattgtc gcagtgccaa cctattctat cttgaaagaa
    46081 atttctaagt tcttatcccg tttgtatgaa aatcataaaa taatgaaaga acgagaaaga
    46141 gaattagcta agtaaaagtc aggagaaccc tgatttttct ttactggaag tggcctttag
    46201 attagaagac tgaaaataag ttaaagtctt aaactaattt tcacagctaa gaatagtaga
    46261 agttaatctg ataaaaatcg aaaaaaccag tggaattccg tgtcagggta agttccactg
    46321 gttttcatag tctattaaag ttcgaatgaa acctatttat agtatattga aactagaata
    46381 gtacacctct aattctaaaa cattgttaga aatcgatttg actgtcctga tctattcgtc
    46441 ctattcttat ttcat
//
LOCUS       DAVKJU010000007        46021 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVKJU010000007 DAVKJU010000000
VERSION     DAVKJU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.007"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(167..517)
                     /locus_tag="VW191_000314"
     CDS             complement(167..517)
                     /locus_tag="VW191_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623152.1"
                     /translation="MEHLFKMIILLPCFYFFSWIERANRDSKIFSIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGVHKGRCRN"
     gene            complement(657..992)
                     /locus_tag="VW191_000315"
     CDS             complement(657..992)
                     /locus_tag="VW191_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623153.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(1081..2391)
                     /gene="der"
                     /locus_tag="VW191_000316"
     CDS             complement(1081..2391)
                     /gene="der"
                     /locus_tag="VW191_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU9623154.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(2405..3118)
                     /locus_tag="VW191_000317"
     CDS             complement(2405..3118)
                     /locus_tag="VW191_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEU9623155.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(3115..4011)
                     /gene="dnaI"
                     /locus_tag="VW191_000318"
     CDS             complement(3115..4011)
                     /gene="dnaI"
                     /locus_tag="VW191_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEU9623156.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(4012..5181)
                     /locus_tag="VW191_000319"
     CDS             complement(4012..5181)
                     /locus_tag="VW191_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEU9623157.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDELRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKAKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(5182..5655)
                     /gene="nrdR"
                     /locus_tag="VW191_000320"
     CDS             complement(5182..5655)
                     /gene="nrdR"
                     /locus_tag="VW191_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEU9623158.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            5795..6160
                     /locus_tag="VW191_000321"
     CDS             5795..6160
                     /locus_tag="VW191_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9623159.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            6165..6860
                     /locus_tag="VW191_000322"
     CDS             6165..6860
                     /locus_tag="VW191_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623160.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            6877..7644
                     /locus_tag="VW191_000323"
     CDS             6877..7644
                     /locus_tag="VW191_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623161.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELTFIAIFYLGTYYILRNKVNLL"
     gene            complement(7997..9196)
                     /locus_tag="VW191_000324"
     CDS             complement(7997..9196)
                     /locus_tag="VW191_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623162.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(9193..10086)
                     /locus_tag="VW191_000325"
     CDS             complement(9193..10086)
                     /locus_tag="VW191_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623163.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEISLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            10571..10790
                     /locus_tag="VW191_000326"
                     /pseudo
     CDS             10571..10790
                     /locus_tag="VW191_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            11205..11822
                     /locus_tag="VW191_000327"
     CDS             11205..11822
                     /locus_tag="VW191_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEU9623164.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(11865..12752)
                     /locus_tag="VW191_000328"
     CDS             complement(11865..12752)
                     /locus_tag="VW191_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9623165.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNVGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(12901..14784)
                     /locus_tag="VW191_000329"
     CDS             complement(12901..14784)
                     /locus_tag="VW191_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9623166.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGVFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGFFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            15161..16615
                     /locus_tag="VW191_000330"
     CDS             15161..16615
                     /locus_tag="VW191_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9623167.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLIHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDKNWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSHAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            16596..17561
                     /locus_tag="VW191_000331"
     CDS             16596..17561
                     /locus_tag="VW191_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9623168.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(17597..18871)
                     /locus_tag="VW191_000332"
     CDS             complement(17597..18871)
                     /locus_tag="VW191_000332"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEU9623169.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(18871..20043)
                     /locus_tag="VW191_000333"
     CDS             complement(18871..20043)
                     /locus_tag="VW191_000333"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEU9623170.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(20174..20422)
                     /locus_tag="VW191_000334"
     CDS             complement(20174..20422)
                     /locus_tag="VW191_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623171.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(20490..21115)
                     /locus_tag="VW191_000335"
                     /pseudo
     CDS             complement(20490..21115)
                     /locus_tag="VW191_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(21177..21626)
                     /locus_tag="VW191_000336"
     CDS             complement(21177..21626)
                     /locus_tag="VW191_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9623172.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(21628..22386)
                     /locus_tag="VW191_000337"
     CDS             complement(21628..22386)
                     /locus_tag="VW191_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEU9623173.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(22501..24480)
                     /gene="pknB"
                     /locus_tag="VW191_000338"
     CDS             complement(22501..24480)
                     /gene="pknB"
                     /locus_tag="VW191_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEU9623174.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(24477..25217)
                     /locus_tag="VW191_000339"
     CDS             complement(24477..25217)
                     /locus_tag="VW191_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU9623175.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDEE"
     gene            complement(25232..26545)
                     /gene="rsmB"
                     /locus_tag="VW191_000340"
     CDS             complement(25232..26545)
                     /gene="rsmB"
                     /locus_tag="VW191_000340"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEU9623176.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLLAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(26538..27473)
                     /locus_tag="VW191_000341"
     CDS             complement(26538..27473)
                     /locus_tag="VW191_000341"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU9623177.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(27486..29882)
                     /locus_tag="VW191_000342"
     CDS             complement(27486..29882)
                     /locus_tag="VW191_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU9623178.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVVKRAYEVMNILRSSLSETSSILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(29948..30262)
                     /locus_tag="VW191_000343"
     CDS             complement(29948..30262)
                     /locus_tag="VW191_000343"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU9623179.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(30287..30913)
                     /gene="gmk"
                     /locus_tag="VW191_000344"
     CDS             complement(30287..30913)
                     /gene="gmk"
                     /locus_tag="VW191_000344"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU9623180.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVSLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(31043..32656)
                     /locus_tag="VW191_000345"
     CDS             complement(31043..32656)
                     /locus_tag="VW191_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU9623181.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(32847..33101)
                     /locus_tag="VW191_000346"
     CDS             complement(32847..33101)
                     /locus_tag="VW191_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEU9623182.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGACSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(33105..33359)
                     /locus_tag="VW191_000347"
     CDS             complement(33105..33359)
                     /locus_tag="VW191_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEU9623183.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(33597..34694)
                     /locus_tag="VW191_000348"
     CDS             complement(33597..34694)
                     /locus_tag="VW191_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEU9623184.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRTQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLYPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(34704..35450)
                     /locus_tag="VW191_000349"
     CDS             complement(34704..35450)
                     /locus_tag="VW191_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9623185.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(35553..35906)
                     /gene="rsfS"
                     /locus_tag="VW191_000350"
     CDS             complement(35553..35906)
                     /gene="rsfS"
                     /locus_tag="VW191_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU9623186.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(35919..36419)
                     /locus_tag="VW191_000351"
     CDS             complement(35919..36419)
                     /locus_tag="VW191_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEU9623187.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(36420..37013)
                     /gene="yqeK"
                     /locus_tag="VW191_000352"
     CDS             complement(36420..37013)
                     /gene="yqeK"
                     /locus_tag="VW191_000352"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEU9623188.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(37013..37642)
                     /locus_tag="VW191_000353"
     CDS             complement(37013..37642)
                     /locus_tag="VW191_000353"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU9623189.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(37694..38005)
                     /gene="yhbY"
                     /locus_tag="VW191_000354"
     CDS             complement(37694..38005)
                     /gene="yhbY"
                     /locus_tag="VW191_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEU9623190.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(38220..39326)
                     /gene="yqeH"
                     /locus_tag="VW191_000355"
     CDS             complement(38220..39326)
                     /gene="yqeH"
                     /locus_tag="VW191_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEU9623191.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVVTRKAII"
     gene            complement(39329..39919)
                     /locus_tag="VW191_000356"
     CDS             complement(39329..39919)
                     /locus_tag="VW191_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEU9623192.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRIQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(39871..40779)
                     /locus_tag="VW191_000357"
     CDS             complement(39871..40779)
                     /locus_tag="VW191_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9623193.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(40836..41396)
                     /locus_tag="VW191_000358"
     CDS             complement(40836..41396)
                     /locus_tag="VW191_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU9623194.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            41535..42753
                     /gene="sstT"
                     /locus_tag="VW191_000359"
                     /pseudo
     CDS             41535..42753
                     /gene="sstT"
                     /locus_tag="VW191_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            42821..43774
                     /locus_tag="VW191_000360"
     CDS             42821..43774
                     /locus_tag="VW191_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9623195.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAISVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYGQ"
     gene            43918..44298
                     /locus_tag="VW191_000361"
     CDS             43918..44298
                     /locus_tag="VW191_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9623196.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            44397..44618
                     /locus_tag="VW191_000362"
     CDS             44397..44618
                     /locus_tag="VW191_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9623197.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            44635..44949
                     /gene="trxA"
                     /locus_tag="VW191_000363"
     CDS             44635..44949
                     /gene="trxA"
                     /locus_tag="VW191_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9623198.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            45255..45788
                     /gene="queF"
                     /locus_tag="VW191_000364"
     CDS             45255..45788
                     /gene="queF"
                     /locus_tag="VW191_000364"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEU9623199.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            45850..>46021
                     /locus_tag="VW191_000365"
     CDS             45850..>46021
                     /locus_tag="VW191_000365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623200.1"
                     /translation="MFEVVDKTDEVSLKHCFEVVDKTDEVRSKHCFEVADRTDEVRSK
                     HCFEVVDRTDEVR"
BASE COUNT        12949 a        10495 c         8871 g        13706 t
ORIGIN      
        1 tcaattaggg gatctaaagc attttatacc aatgttttat aagttgtatt gtattattcc
       61 atattcaatc cactatacaa gatgaatatt tctctcaagt ttcataatca aaataaaaat
      121 gactgtatct atagctccat aaacaaatca tagatacagt cattttttag ttcctacacc
      181 ttcctttatg cacaccttta gatttcccta gttcccgcct caagcgaatc atttttttga
      241 aaaagacata ggctaaccaa tcactagcaa aagcgataag tagtaataat agccaaatac
      301 cccataactt gatatctgtc aaatttctca agacgatatt gaaaaacaga actgaaaaaa
      361 ctgtccaagc aaggctaaaa agagcataga gggtgatgta aatccagtaa aaatagtaaa
      421 aaattgagaa aatcttacta tctctgtttg ccctttcaat ccagctgaaa aagtaaaaac
      481 agggtaataa gattatcatt ttaaacaagt gttccatcac aaacctcctc ttttaaattg
      541 ttttctttag tttcgttcta ttaaatgtta ttaccaatat aaaccttgga atagcgtgta
      601 ataaatgatt ttagaggtac cataagccac ctcctacaaa tagaaaccga tataaatcaa
      661 tgccttccac ccttagactt ccctagttcc tgtctcaagc gaaacatttc tttgaaacag
      721 gaataagtta accaattcat accaatagct agcagaataa aaagaaacca aatgccccat
      781 aacttgatat ctgtcacatt tctcaagacg gtattgaaaa acagaactga aacaactgtc
      841 caagcaaggc taaaaagaga atagaagggg atgtaaaacc agtaaaaata ataaaaaatt
      901 ggaaaaaact tactatttct gttggccttt tcaatccagt tatcaaaata aaagtacggt
      961 gctaaaagta agaatttaaa caaatgttcc atcaccgaca tccccccttc ttttgatagc
     1021 gttttctatt attttattat atcaaaaaaa tccggaactg tcattccaga ttctactttt
     1081 ttatttgcgt tttcttgcga tgagatgaat cggtgttccc tcaaaaacaa aggccttgcg
     1141 gatttgattt tccaagaaac gcaggtaaga aaagtgcatg agttcttctt cattgacaaa
     1201 gatgacaaag gttggtggtt tggttgccac ttgggtcgca tagaaaatct tgagacgttt
     1261 tcctttgtct gtcggtgttg ggttgatggc aatggcatcc atgatgacat cgttcaagac
     1321 agctgatgga atacgtgtat tttgactttc gctgatttgc ttaatcatct caggaagttt
     1381 gtggagacgt tgcttggtta aagctgatac aaagataatc ggtgcgtaag gcaggtattg
     1441 gaactgctca cggatatctt cttcccagtt tttcatagtg tggttatctt tttcaagcgt
     1501 atcccacttg ttgaccacga taatcatccc tttaccagct tcatgagcaa atccagcgat
     1561 acgcttgtcg tattcacgaa tcccctcttc cgcattgatg accattaaga ccacatctga
     1621 acggtcaata gcacgcatgg cacgcatgac agagtacttc tcagtatttt cataaacctt
     1681 accagactta cgcataccag ccgtatcaat catggtaaac tcttgaccat ctgtatctgt
     1741 aaagtgggta tcaatagcgt cacgagttgt tccagcaaca ggactggcaa tgacacggtc
     1801 ttctcccaag atagcattaa tcaagcttga ttttccaacg ttaggacgac caatcaagct
     1861 aaacttaatg acatctggat tttcttcttc atattcattt ggaaggtttt ccacaatcgc
     1921 atctagtaca tcccctgtac cgataccatg gacagatgag ataggcagtg gttcacccaa
     1981 accgagggca tagaaatcat agatatcatt tcgcatctca gggttgtcca ccttgttaac
     2041 tgcgaggata actggtttgt gggtcttata aagcttacgg gctacgtatt cgtctgcatc
     2101 ggtaattcct tccttaccgg acacgacaaa gacgataaca tctgcttctt ccatggcaat
     2161 ttctgcctgg tgcttgattt gttccatgaa aggagcatcg acatcatcaa ttcctcctgt
     2221 atcaatcatg ctaaaagaac gattgagcca ctcacccgtt gcataaatac ggtcacgtgt
     2281 cactccttcg acatcttcta caatggagat tcgctcacca gcgatccgat taaatagggt
     2341 tgatttccca acattgggac gtcctacaat ggcaatagtt ggtagggcca taatttctca
     2401 ctttctacaa taatttcttc tgttcaagat tttttctagt tgagcttggt tcagcttgac
     2461 caaactgttc tgctagacgc tgactccagc ttgtggtcgc acgcgcccca gcatagtcag
     2521 cctgaacacg gtcataagct tggattgcct cagttgactg ttcttggtat tcttcctcaa
     2581 agactacatt atctagtggc agtcttggtt tcacatcatg agtttgattt ggaacaccta
     2641 gtgccatccc aaagacagga taggtgtagt caggcaggtt aaagagttct gccacttctt
     2701 ctgacttgta ccgtaccaag ccaataatca caccaccata acccaaactt tcagctgcca
     2761 gcagggcatt ttgtccagcc aaagccgcat ccaccgaact aatcaagaga ccttctacac
     2821 cttggggttg gaaggtgtcg gtatgaagtc gggctccctt ttctgctcgg ttcaaatctc
     2881 cgacaaagag aaggaaaaca gcagactggc gaatggcttc ttgaggtacc aattcataca
     2941 aggcatcttt cttctcttga cttcgtacca caatcacaga gtaggattgg aaattcttcc
     3001 aagatgatgc catctgggct gctgtcaaaa tctcatttaa gtctacttgg ggaatttctt
     3061 gctctttaaa cctgcgcact gaagtatgag ccttcatcaa tttaatggtt tctgtcatcg
     3121 acggtttact ccttctaaac gagtctcctc agccaaataa cggatgcgtt ccatgacccg
     3181 tctggcttcc caggtttcgt catttccatg tttcactttc gcaaaatgct tctccaaatc
     3241 ttcaaagttg aagttggatg tgaaaaaggt cggtaaattt tcctgcatcc gatattggag
     3301 aatgacctgc aggatttcgt cacgcaccca aacggttgat tgctcggcgc caatatcatc
     3361 taaaatcagg acctcagaca gcttaatctc atccaccaag gtcttaacat tgccatcact
     3421 gatagcattt ttgacatcaa tgacaaagct aggatagtgg aggagagttg atgaaacacc
     3481 acgtttttct gataaatcat gagctaaggc cgccaccatg aaacttttac ccacaccaaa
     3541 gtctccatat aagtaaagac ctttcaaatt attggggtaa tcgttaacaa attctatcat
     3601 tctttttaga acagttaagc gctgaacatc atcacgataa acatctaaaa aactgacatt
     3661 tttcaggcta gatggaaaat taattaagtt gagacgtttc ttgatagccg cttctttttc
     3721 agctgcgatt agttcaggag tttcttcata agaaacatcc gcatagccat ggttcatgac
     3781 taatataggc ttgtagcctt tggcaatata atctgtatct cctcggagga atttatctct
     3841 ctcagtgatg tattggttaa acttggaaat actacggttc aactcgtcct gattgaggga
     3901 ttcttgctgg acaaaggccg caacatcagg atccttcatg attttctgaa ctaaatcttg
     3961 atagtgaaaa cgacttggtt gacgtttgag tacatctccg acactttcca tctaatcacc
     4021 tccgttatct aattttgcta ataattctag ggtttggcgt tccatctctt ctaattcctc
     4081 agcggatgac gtatttttat attcaggatt actccattta ggaatattgg tttttgctgg
     4141 ggcagtctga ttctgttttt gtgtttttgc tttctgtcct cgctcacgga tgcgcaagac
     4201 tgcctcttct gccgaatgaa tcttttgata ggcatagtca ttggctacct tcatggcata
     4261 tttctcattg atatttgccg aatcgacctt attaaaggtc aataaaagaa tgatattgat
     4321 gacttcgtcc agcaagccca agccagccat ctgttgcaag agttctcttt ctgtttgggt
     4381 aatggttccc ttgcgtgttt gcttgatttc tgccaagaac tgcagggcag ttttactttt
     4441 ggcttctttg ataatggttg tttccttaag actaaagtca gaggaaactg gtttttgagc
     4501 aattttttca cgcatgcgtt tggttgaaat aacctgagaa acagctgttg acttggccaa
     4561 ttgataggtt tcaaaccaag tccatttctt ctcctcggca atagcaaaga ggtttaagac
     4621 atcggactgc tcatccgcaa aacgaagttc atctcgagcc atcagctggc gaaaatgttc
     4681 caagtcaaaa tcattggcca ctttcttctt gagaccaagg tcttcttgac tgcctagttc
     4741 tgccaattct ggaaagactt gattgagtga gacaggtatt tcttcaccat cagcactttc
     4801 aactttcaaa tcctccacag cagagtcgcc aatctttttc tctaagagtc tgcgataaac
     4861 aggatgctcc aagaagtctt gactagatag aggagcatgg agggctagct gataaacatc
     4921 ccccttttga tagagagtca agagattaaa agcagataag attttcaatg attttatcag
     4981 tctatccatc ccaaagttga gatgattgag gatacttgaa aaaagatatt cctttctacc
     5041 attatcccaa aaactgattg tataaagata aaggctcagt gcctcctgac cgataatcgg
     5101 gaggtagcac tgtaccagag atgaggtatc ttgcgacacc cgattattct ttagataaga
     5161 aaaacggtca attggcttca tttatctttc ctttttcttt ttagaggact gggtgatttg
     5221 ttggagcaag ctctctaact cactgacatc cttaaaacta cgatagacac tagcaaaacg
     5281 tacataggta atctcgtcca attcagccaa ctcctccatg acgagtgaac caatgtcctc
     5341 actttgaatt tcattttcat ttcgaccacg gagtttctgt tcgatacgat tgactaccat
     5401 gttgatttca tcacttgaca caggacgttt ctgggctgag cggataatcc cattaaagat
     5461 tttatctctg gagaattgtt cccgtgtacc atctttttta acaaccacta aggttctttc
     5521 ttctactcgt tcgtaggttg taaaacggtg ttggcattcg tcgcactcac gtcttctacg
     5581 aatggtgttc ccttcttctg cttggcgact atcgataaca cttgacttgg tagccccaca
     5641 ttttggacaa cgcatccttt ccctccttat cgttttcttt tcattatacc attttttaag
     5701 cgattcccaa aacaattctt ctttttgctt gacaagtttt ttgttttgtt gtattattta
     5761 attaagacaa aagaataaga gaaaggagac taagatgtcc tggacatttg acaacaaaaa
     5821 acccatctat ttacagatta tggagaaaat caagcttcag attgtttccc atacactgga
     5881 acccaatcaa caacttccaa ccgtgaggga gctagctagt gaggctggtg tcaatcccaa
     5941 taccatccaa agagccttat cagaccttga acgagaagga tttgtctaca gcaagcgaac
     6001 aactggacga tttgtgacta aggataagga gctaatcgcc cagtcacgca aacaattatc
     6061 agaagaagaa ttggaacact tcgtttcctc catgacccat tttggctatg aaaaagaaga
     6121 actaccaggc gtagtcagtg attatattaa aggagtttaa gcctatgtca ttactagcat
     6181 ttgaaaatgt atccaaatca tatggagcaa caccagccct tgaaaatgtt tctcttgaca
     6241 ttccagctgg aaaaattgtc ggccttcttg ggccaaacgg ctcaggaaaa acaaccctga
     6301 ttaaactaat taatggcctc ttacaaccag atcaaggacg tgtcctcatc aacgacatgg
     6361 acccaagccc agcaaccaag gccgttgtag cttatttgcc tgatacgacc tatctcaatg
     6421 agcaaatgaa ggtcaaagaa gccctaacct acttcaagac cttctataaa gatttcaatc
     6481 ttgaacgcgc tcatcatcta cttgcagacc tgggcattga tgaaaatagt cgtctcaaga
     6541 aactatcaaa aggaaacaaa gaaaaggttc aactgatttt ggttatgagc cgtgatgctc
     6601 gtctctatgt tttggatgaa cccattggtg gggtggatcc agcagcccgt gcttatatcc
     6661 tcaataccat tatcaacaac tactcaccaa cttctaccgt tttgatttct acccacttga
     6721 tttctgatat cgagccaatc ttggatgaaa ttgtcttcct aaaagacgga aaagtcgtcc
     6781 gtcaaggaaa tgtagatgat attcgctacg agtcaggtga atccattgac caactcttcc
     6841 gtcaggaatt taaggcctaa gcaaaggaga ttatttatgt tttggaattt agttcactac
     6901 gaatttaaaa atgttaacaa gtggtattta gccctctacg cagccgtgct agtcctttct
     6961 gccctcatcg gaatacagac acaaggcttt aaaaatctac cttaccaaga aagtcaggct
     7021 actatgctac tttttctagc tacagtcttt ggtggcttga tgcttacact tgcgatttca
     7081 accattttct tgattattaa acgcttcaaa ggtagtgtct acgaccgaca aggctatctg
     7141 actttgacct tgccagtttc tgaacaccat atcatcacag ccaaactaat cggtgccttt
     7201 atctggtcat tgattagcac cgctgtattg gctctaagtg ctgttattat tctggcttta
     7261 acagctccag aatggattcc tctttcttat gtgattacat ttgtagaaac acatctccct
     7321 cagatctttc ttacaggtat atccttccta ctaaatacca tttcaggaat cctctgcatc
     7381 tacctggcta tttccattgg acagcttttc aatgaatacc gtacagcact cgctgttgca
     7441 gcctacattg gtatccaaat cgtcattgga tttattgaac ttttcttcaa tcttagttct
     7501 aatttctatg tcaattcact ggtaggactc aatgaccatt tctatatggg agcaggtata
     7561 gccattgtta aagaactcac attcatagct atcttttatc tcggaaccta ctacatcttg
     7621 agaaataagg ttaatttgct ttaaataatt tttacctaga tatgtaacat actcatagaa
     7681 caaaagagac caggcaaaaa gtctttaaaa ttagaaaacg catagtatca agtgttaaaa
     7741 atccttgata ttatgcgttt tttgtgagtt gacttatttc ctttcactat atcgcaaaat
     7801 gaaataagaa cggaacgatg ggattttgga attcaaatca atttgtaaga atgttttaga
     7861 agtaatatta tcctattcca gattcagttc actatacaat tgagttttca agcaacttgt
     7921 ttacataatg tgtacataat taggttcgtg attccaccct tttcaccttt aaaaacctcg
     7981 ctttcgcaag gctcttctat ttataagata aggcacgttt aaaggttttc caaatcccta
     8041 aatcatccgt ttgaagaacg agactagcat acatgcgtcc gataaatcct gttgctacca
     8101 ccgcaaaaat cactgtaata gcaagtgaaa tccatgcttc cgctcccccc gcatagccat
     8161 taatcgttcg aaacggcata aagaaggtcg aaataaaggg aatataagaa ccaatcttca
     8221 agaggagatt gtcaccagct gcacctagag ctgtcactcc aaaaaaacca cccataatca
     8281 aaatcatcaa aggcgacaag gctttccctg agtcctcagg acgagaaacc atagatccta
     8341 ggaaggctgc caagactaca tacatgaaaa gactgatcaa aataaagagc agggtattca
     8401 gtgagatagc atctcccaag tgatccaaaa taccagactg agccaagaat ggcaaatctt
     8461 taaagagcaa aacggcagcc agaccaccta caacatagat cccaatatgc gttaaaatca
     8521 ctagaaacag agccatcatc cgcgcataga aatagtgact tgcccttatg ctagaaaaaa
     8581 cgacttccat aattttggtg cctttttcac tggcaacttc ctgagctgtt acacccgcat
     8641 aggtaatcag aatcatataa agaaagaatc ctaaggcacc tgctgcaatt gtttgaataa
     8701 actttttatt ttccttggct tcatcaatct tttctgtgaa ttgaattgtc tgcgctaagc
     8761 gtttttcctg ctcttgagac aaggaagcag ttgaacgatt aagctgattt tgcagttcat
     8821 tgagtgtacc tgtaacctca aatttaattc cattttcaag cgatgtttcg ccatgataaa
     8881 ctgcctttag aacactatct tcttgatcaa tggtcaaata accttttaat ttttcttctt
     8941 taattgcttc tttggcactt gcttcgtctt tatagtcgaa gttaacacca tttacattct
     9001 tcagtccttc tgctacagat ggcactgttg tcactactgc cactttatta tttttagcca
     9061 tagaagaacc ttggagatgc ccaattccta cagagattcc taaaaagagg aacggcgaaa
     9121 tcaccataaa gaagaaactc catgactcga catgtcgaag ataggtttcc ttgattacaa
     9181 cccacatatt tctcatactt ccactcctga ttctagttta aagatttcat cgatagttgg
     9241 cgcttgttgg tcaaatgttg cgatatattg accttgagtc aagattggga agagttccct
     9301 tccagcgctc tcatcctcca aaatcaattt ccaactgcct tgtttggtca agctcacctg
     9361 tttgacatga ggaagatttt ccaattcttc cttgcttcgt tcacttgaaa caaagagacg
     9421 cgttttcccg tattgattgc ggacatcctg aactggtccg tgcaagacca cacggccatc
     9481 tcggatcatc agaatatcgt cacaaagttc ctcaacattg gtcatgacat ggtcagaaaa
     9541 gataatggtt gctccgcgct ctttttcctg aaaaatgact tgtttgagca attctgtatt
     9601 aactgggtcc aatccactaa aaggctcatc caagataatc aagtctggtt catgaatcag
     9661 agtaataatg agctgaattt tctgctgatt tccttttgac aaactcttga ttttatctgt
     9721 cagctttcct ttcacttcca acctcttcat ccattgaggg agtttttcct tgacctcctt
     9781 ggcatccatg ccctttagag tcgccaagta gcgaacttgc tcaaggactg tcaatttagg
     9841 catgagactg cgttcttcag gtagatagcc aatccgagca taagtttcct gacggatatc
     9901 ctgcccatcc agactgattt ctccttggta ttctaaaaat ttcaaaatac tgtggaaaat
     9961 tgttgttttc ccagcaccgt ttttcccgac tagtcccaaa atacgacctg gtcgtgcctg
    10021 aaagtcaata ccaaacaaaa cttgcttgga tccaaaactt ttctctagac ttcttacttc
    10081 tagcatcttt cacctccgaa atgtcttgca ctcattatac tcctttttga tagcctttac
    10141 aatgatttct gtccattttt agaagactat tgctgtgtaa aatatggcct ggagcgtttt
    10201 tagtgataaa tccattatac agtagcaaac tcaatttatc acctctgctc ctcaactgtc
    10261 tatttttgat cctgaattgt tatttgagta actccttttt cctcataaag ttttcttcct
    10321 ctaaaacttc tggaaaaagg cgaatagttt cagacaacat ttttataaga aacaagttca
    10381 tctgtcattt caagaaggag taatccttta tttactaatg gacggaacgg aattcaaccg
    10441 cttgtccgat atgttttcta aggattatat agtaaaatga aataaaaaca ggacaaatag
    10501 atcgggacag tcaaatcgat ttctaacaat gttttagaag tagatgtata ctattctagt
    10561 ttcaatctgc tatatctatt atgcacaccc ctataggatc taatgaaaat cacaacaggc
    10621 tcattcatag atggttacct aagcctaagg gaactaagaa aacgactacc aaggaagtcg
    10681 cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttgaactac aagtccccca
    10741 gagaagactt ctggatgact aacttgaact tgaaatttag caataattaa ttcaccatct
    10801 aactatattt agtaattatt tcagaactga ttaatattaa aattaactaa cgattcaaag
    10861 gattcatact agccataaat tacgtccatc agagagagac tcttactact tttagatttt
    10921 agtctttcta gcttcagaat acatctaaac tttagggaaa atgactattc gaaagcgcga
    10981 atgcctcaaa attatctcag ataagctatt cgaaacttag aatgctttta aatttatgga
    11041 attgcgatta ttcgaaacct agaatgcata taacctttag ttgacagacc tattctaagt
    11101 ctcgaagggc tatttacttt ctattcctta tcaaaaaaga ctcattcccc ctttctcctc
    11161 caaaatatgg tatagtagaa atatactatc tatgaggagt ttacatgtca caggataaac
    11221 aaatgaaagc tgtttctccc cttctgcagc gagttatcaa tatctcatcg attgtcggtg
    11281 gggttgggag tttgattttc tgtatttggg cttatcaggc tgggatttta caatccaagg
    11341 aaaccctctc tgcctttatc cagcaggcag gcatctgggg tccacctctc tttatctttt
    11401 tacagatttt acagactgtc gtccctatca ttccaggggc cttgacctcg gtggctgggg
    11461 tctttatcta cgggcacatc atcgggacta tctacaacta tatcggcatc gtgattggct
    11521 gtgccattat cttttatcta gtgcgcctat acggagctgc ctttgtccag tctgtcgtca
    11581 gcaagcgcac ctacgacaag tacatcggct ggctagataa gggcaatcgt tttgaccgct
    11641 tctttatttt tatgatgatt tggcccatta gcccagctga ctttctctgt atgctggctg
    11701 ccctgaccaa aatgagtttc aagcgctaca tgaccatcat cattctgacc aaacccttta
    11761 ccctcgtggt ttatacctac ggtctgacct atattattga ctttttctgg caaatgcttt
    11821 gacacgtaaa aaatccgttt ggtttcccaa gcggattttg tgctttattt tgaaacttct
    11881 tttgcaagaa caaagttccc aagtgtggca gaaccatttc ctgcgactgc tggcgtcacg
    11941 atatagtcac gcacatctgg tactggtagg taaccattaa gaagagatgt aaatttctca
    12001 cggacacggt ccagcatatg ttgttgagcc atgacccctc caccaaagac aatcacgtct
    12061 gggcggaaag tcactgtcgc attaaccgca gcttgagcga tatagtaggc ttgaacatcc
    12121 caaacagggt tgttgagttc aatagtttcc ccacgtacac ctgtacgagc ttccaaactt
    12181 ggaccagctg cataaccttc tagacatccc ttatggaaag gacaaacacc cttaaactct
    12241 ttttcaatat ccattgggtg tctagcaaca taataatgac ccatttcagg gtgacccaca
    12301 ccaccgataa actcaccacg ttggatgacg cctgcaccga tacctgtacc gattgtgtag
    12361 taaaccaagt tttcgatacg accaccaaca ttgttacggg caaccacttc accgtaagca
    12421 gagctgttta cgtctgttgt gaagtacatt ggcacgttta gggcgcgacg aagggcacca
    12481 agcaagtcca catttgccca gtttggtttt ggagtcgtcg tgataaagcc ataagttttt
    12541 gagtttttgt caatatcaat cggcccaaat gaaccaactg caagaccagc aaggttatcg
    12601 aattttgaga agaactcaat ggttttatcg attgtttcga ttggagttgt tgttggaaat
    12661 tgtgtttttt ctacgacgtt aaagttttca tcaccgacag cacagacaaa ctttgtaccg
    12721 cccgcttcca agcttccata taattttgtc atgataaacc tcttgttttt attttcttta
    12781 ttatagcata cttcgaaagt ctaaatgtct ctatttttta gattttcctc tgtaaatctt
    12841 actatctaat aaaaacgaac aaacatgtca tttgttcgtt ttcacattag agaggattga
    12901 ttagattttc acttcgatca cagcatcccc cttcgcaact gaacctgttg cgactggagc
    12961 tactgaagcg tagtcagctg tatttgtaac gataaccatt gttgtatcat caagtccagc
    13021 tgcagcgatt ttgtttgagt caaatgttcc aagaacatcg ccagctttca ccttattacc
    13081 ttgagcaact tttgcttcaa aaccgtcacc gttcatagat acagtgtcaa taccaacgtg
    13141 aatcaaaact tcagcaccat ttcttgtttt caaaccaaaa gcgtgccctg ttggaaaggc
    13201 aattgaaact tcagcatcag ctggtgcata gaccacgcct tggcttggtt tcacagcgat
    13261 accttgtccc atagctccac ttgagaagac tgggtcattg acatcagcaa gagcgacaac
    13321 atcaccaacg ataggagtta caagtgtttc attttgaaga gctgctggcg caacttcttc
    13381 tttttcttca gccacttcag cttgttttgc agctgcagtt gcgtctactt catcttcgta
    13441 accaaacatg taagtaagag caaaaccaag ggcaaatgat acagctacca taagaaggta
    13501 ttgtggaagt tgtccgttac caacataaag cattgtacca gggatgatgg tgataccatt
    13561 accagtacca gcaagtccaa ggatagaagc caatccacca ccgattgcac cagcaatcaa
    13621 tgaaaggaag aatggtttac ggaagcgcaa gttcaccccg aagatagcag gctctgtaat
    13681 acctaggaag gcagaaagag cagccgggaa agcaagtgtt ttcagttttg gattttttgt
    13741 tttaacacca accgcaacag tagcagcacc ttgagctgtc atagcagctg tgatgatagc
    13801 gttgaatggg ttagcatggt cagcagcaag taattgcact tcaagcaagt tgaagatgtg
    13861 gtgcacacct gacacgacga tcaattggtg aaccccacca atcaagaaac caccaagacc
    13921 aaatggcatg ctaagaatcg cttttgtagc aataaggatg tagttttcaa caacgtggaa
    13981 aactggtcca atgacaaaga atccaaggat agacatgacc aaaagtgtca cgaatggtgt
    14041 taccaagagg tcaatgacat ctggaacaac cttgcggaca gctttttcaa atttagctcc
    14101 gacaaccccg atgatgaagg ctggaagaac ggaaccttgc aaaccaacaa cagggatgaa
    14161 accaaagaag ttcatcgctg ttacttcacc accttgagca actgcccaag cgtttggaag
    14221 tgagccagag acaagcatca taccaagaac gataccaacg gcaggatttc caccaaatac
    14281 acggaaggtt gaccacacaa ccaaacctgg caagatgatg aaggctgtat ctgtcaagat
    14341 ttgtgtgtaa gttgcaaagt cacctggaag tggcatttca agagcgttga aaagaccacg
    14401 cacacccatg aagagacctg tcgctacgat aactgggatg attggaacga aaacatcacc
    14461 gaaagtacgg atagcacgtt ggaaccagtt cccttgttta gcaacttctg ctttcatgtc
    14521 atccttagat gatgttggta agccaagtac aacaacttca tcgtacattt tgttaactgt
    14581 acctgtacca aagataattt ggtattgccc tgagttaaag aaaacacctt gaactttttc
    14641 caagttctca atcacttctt tattgatttt ctcttcatct ttgaccatga cacgtagacg
    14701 agtcgcacag tgggcaacac tattgacatt ttcacgtccg cccaaggcat cgatgacttt
    14761 ttttgcaatt tcctgattgt tcatttgcaa aaatctcctt atataacatt ttgttcttgt
    14821 ttgaaagcga ttttattcgc ccttacgact attattttat catgttttaa aaatatgtca
    14881 agcgttttgc ataaaaaata ttctatccac ttaatgggca agctttagat tgattgtttt
    14941 tgactgtttc atcaggaatt ccttgaaaaa taagtttgaa accttggtta aaaagtcttt
    15001 tatttcattg tctttcacat tttcgtaaaa atttaattga tttcctgtat ttattttatg
    15061 ataatcgttt gacagttttt tctctttttt aacataaaag gcttgcattt tttatcgaaa
    15121 acagttacta ttaaaagtga taagattttt ggaggaatga atggaatgga caactgagcg
    15181 tcgttacaga ctttatcaag attggacgca agaagaaatt caacatataa aggaaaatat
    15241 ggcacaatct ccatggcata ctcattacca tgttgagcca aaaacaggac ttctcaacga
    15301 cccaaatggc ttttcttact ttgatggcaa gtggatcctc ttttaccaga attttccttt
    15361 tggtgcagcc cacggtttaa aatcttgggc acagctagaa agtgatgatt tgattcactt
    15421 taaagaaact ggaatcaaag ttttaccaga tactccatta gatagccacg gtgcctactc
    15481 tggttctgcc atgcaatttg gcgataactt attcctattt tatacaggaa atgttcgcga
    15541 taaaaactgg atccgtcacc cataccagat cggtgctttg atggacaagg agggtaagat
    15601 tacaaagatt gacaagatct tgattgacca gccagcagac tctactgacc acttccgcga
    15661 tccacaaatt tttaactttc agggtcaata ttatgccatt gtcggcggac aagacttgga
    15721 gaaaaaaggt ttcgttcgtc tctacaaggc tgtcaataac gactacacaa actggcaagc
    15781 agttggcgac cttgactttg ctaacgaccg tactgcctac atgatggaat gtcctaatct
    15841 ggtctttgta gaggaacaac ctgtccttct ctactgtcca caaggattgg ataagaaagt
    15901 tctagactac gataatatct ttccaaatat gtataagatt ggggcttcct ttgaccctaa
    15961 aaatgccaaa atggtagatg tgtctcaact tcaaaacatg gattacggtt tcgaagccta
    16021 tgcaactcaa gccttcaacg ctcctgatgg gcgtgctcta gcagttagct ggcttggttt
    16081 gccagatgtt tcttacccat ctgaccgttt tgaccaccaa ggaaccttct ctttggtcaa
    16141 ggaactcact atcaaagacg acaagctcta ccagtatcca gtcgctgcta ttaaggacct
    16201 tcgtgcttct gaagaagcct tctcaaaccg ttcccaaacc aagaacactt acgaacttga
    16261 actcaacttg gaagctaata gccagagcga gattgtctta cttgctgata aagaaggtaa
    16321 gggactttca atcaactttg accttgtaaa cggtcaagtg acagtggatc gtagccatgc
    16381 tggagaacag tatgcccaag aatttgggac aactcgttct tgccctatcg agaatcaggc
    16441 tactactgct acaatcttca tcgataactc tgtctttgaa attttcatca ataaaggaga
    16501 aaaagtattt tctggtcgtg tcttcccaca tgcggaccaa aatggtatcc tgatcaaatc
    16561 tggaaaccca actggaactt actatgaatt agattatggt cgcaaaacta actgatgtcg
    16621 ccaaacttgc aggcgtcagt cctactaccg tttctcgggt tatcaataaa aaagggtatc
    16681 tatctgagaa aaccatccaa aaagtcaatg aagccatgcg agaattgggc tataaaccca
    16741 acaacctggc tcgtagtctg caaggaaaat cagctaagtt aatcggcttg attttcccta
    16801 atatttccaa tgttttctat gcagaattga ttgataaatt ggaacaccaa ctcttcaaaa
    16861 atggttacaa gaccatcatc tgcaacagtg aacatgattc tgagaaggaa cgcgaataca
    16921 tcgaaatgtt ggaagccaat caggtggacg gcatcatttc tggtagtcac aacctaggaa
    16981 tcgaagacta caatcgtgtg acagcgccga ttatttcctt tgaccgaaac ctatcgccag
    17041 acatccctgt cgtctcctct gacaactatg ctggtggggt tcttgctgcc caaaccttgg
    17101 tcaagacagg tgcccagtct atcatcatga ttacagggaa tgacaattcc aattcgccaa
    17161 ccggactacg ccacgctggt tttgcatccg tactcccaaa agctcctatt atcaatgttt
    17221 ccagtgactt ttctcccgtc agaaaagaaa tggaaatcaa gaatatcttg acccgggaaa
    17281 aaccagatgc catttttgct tcggatgatt tgacagctat tctggtcatt aaaatcgctc
    17341 aagaattggg catttctgtc ccaaaagagc tcaaggtcat cggctatgat gggacctact
    17401 ttatcgaaaa ttactaccct caattggcta ctatcaagca acctttggaa gagattgctt
    17461 gtctcactat tgatcttctc ttgcaaaaga ttgaaggcaa ggaagttgcc acaactggtt
    17521 acttcttacc agttacacta ttaccaggaa aaagtattta aacacaagaa aactcagacc
    17581 gattcgtctg agttttttat gatcttaaat tttcgagata gcgctgggct gtctctaggt
    17641 taaaggtttt atctgagatg aggcgctcta ctaggggagc aacttcagat tcactagccc
    17701 cagctaggag agctagggat ttggcctgta gtttcatgtg gccttgctgg atgcccgtac
    17761 ttaccaaagc tttgagggct gcaaaatttt gggcaagacc gatggacacg ataatctggg
    17821 ctaattctct ggcagaagga tttcctagta gatcatgact gaaagctaca cgtgggttga
    17881 ggccgataga gccaccctta gtcgctacag gcatgggcag ggtcatctca ccgaccaatt
    17941 cttctctttc aaggtccagc gtccagcaac taagaccttg atagcgtcca tctcgactgg
    18001 caaaggcatg ggccccagct tcgatggcac gccagtcatt accagtggca atcaaaatcg
    18061 catcaatacc attaaaaatt cctttattat gagtagcagc tcggtaagga tcagcctgcg
    18121 caaactgact agccaacgca attttctccg caatctctcg tccttgatcc ttttggcggc
    18181 tcaagtagcg aaaggccatg cgacagcttg cagtcaccag agaatcggtc gcgtagttgg
    18241 acaggattcc tatgagactc tgtccctgac tgagttcttc taagactggt ttcaaggctt
    18301 ccagcatggt gttgagcata ttggcaccca tggcttcctg ggtatcgaca tgaatataaa
    18361 caacgagaaa gtctggttcg ccttttatct gctcgacatg cagatcacgc gccccacctc
    18421 cacgtttaac gatagaagga taggcttgat tggcaaactc caagagctcc gctttcttgc
    18481 tggcaatctt ctcttgcgct agtttaggat tagcaacttg ataaagggct acctgcccaa
    18541 tcatctggcg ctgatggact tgtgcagtaa aaccacctgc acgcttgatg attttgctgg
    18601 catagctggc cgccgcaacc acagagggtt cttctgtcac atagggaacg gtgtattcct
    18661 gaccgttgac aagtacctcc ggaaccagcg aataaggcag agaaaaagtt cccactacat
    18721 tctcactcag ctggtctgcc acagtcacac tcatctgttc atccttctcc agactagctt
    18781 gtctctcagg actaaggagc gcctgagctt ttagcagctc gaggcgctct tggtatgatt
    18841 ttttagaaaa tccattccaa cttatcttca ttatttttca accttgctat aacggcgttg
    18901 gtggtcgaga atttcaacca aggcaaaatc ttgattttca tagccagcaa actgggcaga
    18961 gtttgtttca tccaagttta cttcctcaaa aaagaccttt tcatagtctg caacggataa
    19021 ggcagttcgt tggttgagct tgttcaagcg gtctttatcc aaataagctt catatccttc
    19081 aaccaattca ccactgaaga actcagccac agctccactt ccgtaactat aaagggcgat
    19141 tttatcccca gctttcaagc tatctgcatt ttccaagaga gacaaaagtc caaggaaaag
    19201 tgaacctgtg tagatattcc ccaccttttg actgtagaga atagactggt caaaatgctt
    19261 ttgtaagagg tcttttttct cttgaggcag gctcttatcc atgatttttt tcaagccttt
    19321 tagcgctaat ttaggataag gcaagtggaa acaaacagcc gcaaaatcat ccagagtaag
    19381 ctggtagcgt ttttgatatt caagccaagt cgttttcaaa ctatccaagt attgttgggt
    19441 agaatagaca ccatttacat aaggagttgt cgagtaattt ggtcgccaga aatccatgat
    19501 gtcacgggtc tgagctacat tgtcattatt aaaggccatc atgcgtggat tttgtgtaat
    19561 caacatagct acacttccag caccttgagt tggttctccc ggagtttcaa taccatattt
    19621 ggcaatatca ctggcaatga ccaagacctt ggactccgga gaattttcca catgcaattt
    19681 ggcataatgg agggcagctg tcgccccata gcaggcttct ttaatctcga aactacgagc
    19741 aaagggctgg atgcccagca agccatgcac aaagacggcc gcagccttac tctggtcaat
    19801 tcctgactcg gtagccacaa tgaccatgtc aacttcttgt ctttcttgct cagttaaaat
    19861 agagtcacta gcactggccg ccaaggtcac gatgtcctca gttaggggcg caatactcaa
    19921 ttccttgagt aagagtcctt tacttaattt ttcagggtca attcccctcg cttctgctaa
    19981 gtcttgtaat ttcaagacat attgactggt cgcaaaacca atcttatcaa taccgattgt
    20041 catatttacc tctgttttat cattcatgta aaaaatcgtt ctatactatt ttatcacaaa
    20101 tggcagtaaa agagagaaaa aagacttgat tcaccaaatc aagcctctta ttggtcatca
    20161 ttttaaagaa tgattagttg ctagagagtt caccgatata agtagcttta taagctccat
    20221 tcacagttat cagctcctgg aggatcaaat ttcctgagta aatccttccc atctcatcta
    20281 caaatttttg ataaaactga ctggtcggaa tttctctgac atccttatca aatgtcttat
    20341 caagtgtttt actaaccttc tcagcaatca attgatgctc ttgccatcca ctttgaaact
    20401 ctgagcccga actagaaacc atgactggga taaacaacaa ggtcagtaga tttacagaca
    20461 ataaggaaag tagtagactt cctgcaaaac tagaatccta gttcatgatt gataatgcca
    20521 gcaatcaaat tcattcgtaa tccgaagcgt ttacgatgat ttcgataggt tgttgaaaac
    20581 attttaaacg tttttacttt ggcaaagatg ttctcaacct tgcttctctc cttagatagc
    20641 gcatggttat aggctttatc ttcaactgtt agcggcttga gtttgctgga tttacgtgga
    20701 gtttgtgctt gaggatatat cttcatgagc ccttgataac cactgtcagc caagatttta
    20761 ccagcttgtc cgatatttct gcgactcatt ttgaacaact tcatatcatg acaatagttc
    20821 acagtgatat ccaaagaaac aattctccct tgacttgtga caatcgcttg agccttcata
    20881 gcgtgaaatt tctttttacc agaatcattc gctaattgtt tttttaggac aattgatttg
    20941 tacttccgtc gcatcaatca ttaccgtgtc ttcagaactg agaggagttc ttgaaatcgt
    21001 aacaccacct ttgaacaaga gttacttcaa cccattggct ccgacggagt aagttgcttt
    21061 cgtggatacc aaaatcagcc gcaatttctt cataagtgcg gtattctcgc acatatagaa
    21121 agcgttgtta atttatttat ctcatttttc agaaaattct tttatttctg taaagtctac
    21181 gatactcgat gtgtttttat ataatgatac agtctgagaa tcactgttcc gctagccatt
    21241 ccaatagaga ttaccaaagc caacatgaca accaaggtcg cacttgccag tgctttatta
    21301 tagtcccctg tcacaaaaaa ggcagttgtt cggtaggaga gataacctgg aaccagcggt
    21361 gccaaaatgg ccaagataaa gaccacagca ggtgtcttat aaagaatact taaaatctgg
    21421 ctgacacaag aaccaataat ggctgcaatg aaggtagcta caatgacatt ggtcggttcc
    21481 ttgagcaaga gatagattag ccagacagtc atgcccaaaa tccctccagg taagagcata
    21541 gaccgttgca cattgagtac gattaaaaaa gtgataatgg caagaaaact tgctactgct
    21601 tgtaataaaa aggttgttag tgtcatatta gttcatcaat accaaggcga cagaagttcc
    21661 tgcccctaaa gcgagggtaa tgagcaggga ttcaaacatc ttactcatac cagagtttat
    21721 gtggttggtc ataatatcac ggaccgcatt ggtcaaggca atacctggta caaacggcat
    21781 gaccgcacca gctataatca aatctgccgt tgaaggaaaa cctgtgtagc gagcccaaaa
    21841 ctgggcaatt atcccaaaga caaaggctcc agcaaaggct gtcacaaagg gaattcggat
    21901 aaatttttcc acatagaggg aaaaggcaaa accaaataag gtcgccactc ctgccccaag
    21961 tgcgtcgtag atatttccgc taaacataac tgaaaagaaa ggagcactaa aggtcgcagc
    22021 cagagttacc tgcaacttag tatagggaag gggttgagct tgcaaggccg tcaattgctt
    22081 aaaggctgtt tctaagtcaa tctgcccccc aactagctga cgagaaatct ggttcacatc
    22141 gcagactttt tcgatgttat aagaagagga ggtcacgcgc ttcatgcgcg aaatattggt
    22201 attttcaata gagaaaaaga tagcggcagg catggcaagg acattgcaat ccacaatccc
    22261 ctgcgaatgc gcgattcgaa tcatggtatc ttctacacga tggatttctg agccactttt
    22321 aaggagaata gtccccgcta gcataatcac atcaatgacg gcatttaatt cttttgattc
    22381 ttccatgctt tcctcctttt atcaactccc tctattctat cacaaatctg gactcaaaaa
    22441 aaatctttgc catgaaatca tgacaaagat tgattactca ttttgattat ccatctgctt
    22501 ttaaggagta gctgaagttg ttttaggttt gtagattgaa atcttgactc tagtcttatt
    22561 taggtctacc ttttcacctg ctctaggact ttgttcaaca accatgcctt ctacactacc
    22621 tgcaggcgct gtcgtcactt ctacaacttc tatattagct tccttaatcc caacaatttg
    22681 aatcaaattg ttcttagtaa actccaagct ggaaccaatg taactcggca tggcaacact
    22741 tgtaactttt ttagctactg tcaagacaat ttgagtaggt ttactcacat cataagtcgt
    22801 tccggcacct ggactttgtt tcataatcgt tcctggttcg ctttcgctgg actcttcttc
    22861 ctctatctta atcaaattct caggaacctt cttctgcttg agttctgaga ttacttctgt
    22921 agagttccgt ccaatatagt tccctaattg aatcgtcgta gcttttttag ctactgtcaa
    22981 aacaatttga gttgccttgc tcaagtcata ggtcgtacct tctggtagac tttgcttcag
    23041 gaccgttcca gcctcactct cattcgactc ttcttcctca attttaatca aattatctgg
    23101 aacttttttc tcttttaatt ccgcgataac atcagaagat ttccggccga cataattact
    23161 aatttggaag gattgtttgc ctgatgagac aaccagattg attttcgttc cttcttttcg
    23221 accagttcca gcgccaggat ctgtacggat aatccgccct tcttccacct tttcactagc
    23281 ctctgtcttc tcctcaccaa tctcaaaatt ggcttttttg agcgttgcct tggcctctgc
    23341 aactgtctga cctgccacat ctggaatggc aatggttgca ggagttctgg atagtatcca
    23401 aataagagaa gctgccacca atacaaggct ggccaacaaa atcaggtaac gcatcttaaa
    23461 tctatgtttt ttcggtgctt gtggttggta agtttcctct gtcacagcct ggcttgggtt
    23521 tttgattgat ttgtgttctg tctgcgcttg aaccttagga atagatgtca aggtactctg
    23581 agaaaccttc ggcaaggtct tggtatctgc cttgctcgtt tcatcaaaga ttaacttact
    23641 ttcatttcta cgattgtagg acaagctact agacaagtcc acatacatct ctgaaaccga
    23701 gcggtagcga ttggtcaact ttttagcagt tgccttgata ataacatttt ctaaagcctg
    23761 aggtacagat ggattttctg caataacgga cggcaggggt ttctggaaat gctggagggc
    23821 aatggtcacc gcgctatccc cgtcataagg gatatggcct gtcaacatct catagaaaat
    23881 aatccccatg gcatagatat cactctgcac agtcgccttc gaaccacgcg cctgctctgg
    23941 tgacaagtaa tgaactgagc ccaacatcga gttagtctgg gtcagacttg tctctgcaaa
    24001 ggctacagca atcccaaagt ctgtgacctt ggccgtccca tctggtgtca aaaggatatt
    24061 ttgaggtttc aagtccctgt gaacaattcc tcgagtatgg gccaagcgca tagccaagag
    24121 aatttgtccc atgatacgga ctgcttcttc attagaaaga ggataatgtt ccttgatata
    24181 gcgtttgagg tctagtccag caacatactc cattgcaaga tactgttgac cgtcttcctc
    24241 accaatatct gttatccgaa cgatatgagg atggtctaga tctgccatag ctctcgcttc
    24301 acgctgaaaa cgagctacag ctatcgggtc cgtctggtag ttggtcctca gaaccttcac
    24361 tgccacttct tccccatcta agattaagtc tttggctagg tagacatccg ccatgcctcc
    24421 tcgaccaatc tgtttgacaa tccgatagcg tccggcaaaa atcttgccga tttggatcat
    24481 tcttcatcct cctcgttcat agaaacaagg gcaaccgtaa tgttgtctaa acctcctgca
    24541 ttgttagcaa aacgaacaag tgtctccgtt ttatctgcta aaggaatatc actggttaca
    24601 atatcacgaa tctcactgcc tgaaatcatg ttggtcaagc cgtcactatt gagcaagaga
    24661 tagtcacctg actcaaggat aactgtccca aaatcaggct gaatttcatc tttttgccca
    24721 atagactggg tgataatatt tttttgcgga tgagcttctg cctcttctgg tgtcaattga
    24781 ccagccttga gcaattcatt aaccaaggaa tgatcgctcg tcaactgatg gtattcttct
    24841 ccacgaatca agccgatacg cgaatcacca atatgagcat agatagcctg attatcaata
    24901 atagcaagga cttccaaagt agttcccatg cctctgtaag cttcatcctg accaagctgg
    24961 tgaatctttt gattttcaat ttctaggtaa tgggcgaacc attcacgcac ttcattgact
    25021 gtatcgatct gggtatcaac ccaagctaca cccaggtctg tgaccgccat ttcactagcg
    25081 atattccctg cacgatgacc tcccatccca tcagctaaaa taatcatggt acgtccagct
    25141 ctattgacat agtggttgac atagtcttgg ttatttgttc gtttctgacc aacatctgtt
    25201 aataatgaaa tttccatgtg tcagttcctt cctaatccga tatcttgcga aattgactga
    25261 tgaagaatcc atcacttcca tacaattcag gtgtaatgag gatacagccg tctttcatga
    25321 tatccttaca ttcatgttct agttttacct gctcgaactc gggatgactc tctaaaaagg
    25381 ccttaacaac ttgaaaattc tcctctgaga cgatagtgca ggtgctataa gttattatac
    25441 cacctttgcc tagtatttga caaacactac ctaatatttc taactgaatt tcctgcaagg
    25501 acgcgaaatc tgccgtttct ttattgtatt tgatatctgg ttttcggcgc aaaagaccga
    25561 ttcctgaaca aggagcatcc accaaaatct tatcaaagga atcctggtca aaaaactcat
    25621 gcacctttct ggcatccaat ttttgagttt gaacccgatc ttcaactccc agacgttggg
    25681 cattttcttg aattaaatcc aacttgtggt cgtacaagtc cagagcagta acctgacctg
    25741 tcgtaagata agaggctata tgggctgttt tcccacctgg agccgcacag gcatcaagca
    25801 ctcgctcatc accttgtaaa tcaagcgtcg gagcaaccaa ctgactggac tcgtcttgga
    25861 tggtaatggc tccatccgca aacaaattat gccctgcaaa atgcccctgc tccttaacca
    25921 gaccagtggt tgctaaaagg gaattattcg cctccaacaa ggcttggatt tcctcttttc
    25981 gacttaggtc tgttacacga atactggctt tgtttcgcac taacaggctt tcaaagatgg
    26041 cttttgctct ctcctctccg tattcttcct tgagtttggc aactagccaa actgggagag
    26101 aataggcaat ggagtcacgc ttgttttttc gcttgatgct agcaatatct ggccagcctt
    26161 cacgcaagat acggcgaagg acagcgttga ccaatttttc actgcctttt ttacggagtt
    26221 tggccaattc cactgcttca ttgaccacag catgatctgg aatcttgtcc aaatagcgga
    26281 gttggtaggc actcatgaga agaaggacat agagccagct gtctaactgg tctctgtctt
    26341 cgataaagtg ggataggtac cattccagag tcagtttacg ggctaccgtt ccatagacca
    26401 gctcggtcac taagcccttg tctgctgcca aaagttgact tccctttaga tgcttattta
    26461 aggcgatatt tgagtatgct tggttcacaa aaacatcctc tagcactgct agagctaaac
    26521 ttctagccgt ttctacttta gtcaccaaat cgttctccta cagtcaatgt acgtccaact
    26581 ccgttgagga aggaagcaat gtccatctta ggcttaccag ctggctgcac ttgtttgagg
    26641 gatagagccc cttcagccgt tgcgacaatc aattctttct tgccgataga gagaatctca
    26701 cctggatttc cctgaccttc tactggtagg gcttcataaa tcttaaagcg gtcgccctta
    26761 aggaaagtat gggcaacagg ccaggggttc attccacgaa tttggttaaa gagttgacga
    26821 ttggttttgt tccagtccag tttttcttcc tctggcttta tatttggaga gaaggtaacc
    26881 tgactcgtat cctgcggttc aggtttgata tcaccagcaa tataggcagg cagagtgtcc
    26941 aaaagcaaat cacgaccaac tagcgccaat ttttcaaaca aggcgccaac attgtcctca
    27001 tctgtgatcg gaatgctgcg acgagaaatc atatctcctg catccatttc cttaaccatt
    27061 tccatgatgg tcacaccagc ttcctcatcc ccttgaatca aggcataatg gataggcgca
    27121 ccaccacggt gtctaggaag gagggaggca tgaacgttga cagcaaagtc catgctatca
    27181 aggagtttgc ttgggagaaa ctgcccaaaa gcagcagtca caattccatc tgctcctagc
    27241 ttcataagat cttccatctc tggacttcca gataattttt caggttggta gatagatagt
    27301 cctgcttcct tggcagcctg cttgactggg gtttcttgga taactttttt acgaccaaca
    27361 gcacggtctg gctgggtcac aacggctaga atttcgtaac ggtcatctgt caaaagtcct
    27421 tttaagactg ttgctgaaaa gtcgggggtc cccataaaga ttagttttgt catatcttct
    27481 ccttcttata aaaattgctg cggctcatgg tcaatgctga gacggagctc actattttcc
    27541 cgttcttgag tcaaggccag gacctggttg agggtcggcc ccagctcatc ttctaaacgg
    27601 tatttaatta aaatctggta atgatagagg ttgtgggtac gggcaatcgg ttttggcgtt
    27661 ggccccagaa tggaactggt ctctgacaag cttgaccgca aaatattcat gacttcatag
    27721 gcacgtttga ccacctcttc ttctttcttg tgagaaaggg taataccaat cgtgaaatag
    27781 taaggtggat agccgagttg tcgtctgatt cccatttcat aggcataaaa gccttcgtaa
    27841 tcttgatcct tggcaaatcg aatagcatag tgctgcggat tgtaggactg tatcaagact
    27901 tgacctgcct tttcagcccg acctgctcga cctgccacct gagtcaagag ctggaaggtt
    27961 ctctcagaag aacggaaatc aggcagattc aaggccgtat ccgcatttag aactccgact
    28021 agggtaacat tgggaaaatc caaccccttt gcaatcatct gagtaccaag taaaatatcc
    28081 gcttcccctc gcccaaactg gtcaagcaag gcttggtgac tgcctttctt tcgagtcgta
    28141 tccacatcca tcctcagaat gcgagcttgg ggaaagagtt ctgctagctc atcataagcc
    28201 ttctgagttc ccgtcccata gtaacgaata ctgcggctct tacagttagg acagacctga
    28261 ggaatatcct tcgagaaacc acaataatgg cagttcatag tcttggtatc catatgcaag
    28321 gtcagagaaa tatcgcagtt gggacaagta tccaccgtcc cacactcccg acacatgaca
    28381 aagctagaat aaccacggcg attgagcatg agaaccacct gctctttttt aaccagacgg
    28441 tcttggatag cctctagcaa aggaggcgta aagtttgacg tctcattttg tccgatatag
    28501 tctcgaaagt caatcacttg aacctcaggg attgtagcca aaggattggc acgttgggtt
    28561 agacgtaagt gttgatagac gcctttgcca gcacgtgccc ggctctctaa gctcggcgtt
    28621 gcagatccaa gtaccagagt tgcttgatta tactgagccc gtaaaatagc tacctctctg
    28681 gcatggtaac ggggattgct gtcctgctta taagccgctt catgctcttc atcaataatc
    28741 atgacaccca gatttttcag aggagcaaag atagcagatc tggcaccaac aacaacttgg
    28801 gcatcgccac gctccacctt gcgccattca tcatactttt caccattgga taatcctgag
    28861 tgaagaatgg ctaccttgtc cccaaaacgt gctataaaac gctcggtcat ctgaggagtc
    28921 aaggaaatct caggtaccag caaaatagct gtcttgccct tatccagggc accttggata
    28981 atctgcaagt aaacctcggt cttcccactt cctgtaatcc cttgaagtag aaagggaggt
    29041 tgagaactgc caatagaact cacaaccgca tcacgcgcct gtctttgttc tggatttaac
    29101 tccaaaggtc tacttgcttc aattccttca aaataagcag ccgagcgttg aacttccttt
    29161 tggactatgg taacagcacc ttgatccaca aagaagttga cttgctctcg cgagtaggac
    29221 tctaacaagc tagccaagga agcgctctct ggatgagaca gcagataatc tctcagttcc
    29281 aactttttct tggcacgtgt agaaatctca acaccttcta attgagcatg gtcaacctca
    29341 taccaagact gggtcttgac cttcttttga tcgactgcct gatattccag accaagcagg
    29401 ccttttctag tcaaacgcat catttcagct tgcttggcaa ggtctagtga agaaaaggct
    29461 agcgaatctt ctgaaccaaa caggcgcact cgttcttcct gactcaagcc ttccagagga
    29521 tagagaatct tgtcatagct agaattcaga aatcctggaa gcatggcctt gaggatagaa
    29581 attttgtagg agaagacaga tttgcgtaac tcctcagcca gccagagttg ttctggcgtg
    29641 agaacaggag aaaaatccag cacctctgca atatctttta aatcttgctc catctcttct
    29701 ccatctgatt gggactccaa accaagaaca atcccttgaa tcaggcgatt acccttacca
    29761 aaaggcacat gaacccgcat cccaacttcc agcattccct caaattcctc cggaatcctg
    29821 taactatagg gctggtccgt ctgcatcaag ggcacatcta cgataatctt agctagggcc
    29881 atcttctcac ctcctccttg tcagtacatt cttgcaatag aaaaaataag attgagtccc
    29941 cccaacctta aattttttca ccatcttctt tttctttagc aatttgctct ttgattttct
    30001 tttcttcttc ttctttgcgg cgtttttctt cttcgatacg gcgacgcact gcttcacgtt
    30061 ttccttctgg atctgggtga attgtaacgt ttcctgattc gatttcttct aaagcgcgaa
    30121 gagttgattt ttcagacttg aaaccttgag ttgctggggc acctgcttcc aattcgtggg
    30181 cacgttttgc ttccaagatt acgagtgaat attttgaagg aaccttgtcg agcaaggtat
    30241 caatagaggg ttttaacatc atttgcttgt acctattttc taaattttat cgggtagttg
    30301 gagattttgg taacatctcc tgatagtgac caatgacacg atccacacag aagtgttctg
    30361 cttcaatcac acatttgaca cgttcagcag ctagggacac ctgatcgttg acaatggcat
    30421 aatcatactc acgcatgagg gcaatttctt ccttggcctt ttcgattcgt tgggcaatca
    30481 cttctgcact atctgttcca cgacctacca agcgatcttg caattcatcc aaatctggtg
    30541 gtgtcaggaa gataaagaca gcatctggaa cctttttctt gacctgaaga gcaccctgaa
    30601 cttcaatttc aaggaaaaca tcgattccct tgtccaaggt ttcattgaca taggtcagag
    30661 gagttccata gtagttaccg acatattctg cgtattccaa catctgtcct tgacgaatca
    30721 gctcttcaaa ttcttcacga gtacggaaga aatagtcaac accgtccact tctccaggac
    30781 gttgtgcgcg tgtcgtcatc gatacagagt attgaaattg gttttcagaa ctctcaaaaa
    30841 tctctcttct aaccgttcct tttccaaccc ctgaaggacc agaaaaaacg attagtaagc
    30901 ctcggtctgc cattgtgtct ccttttagtc aatctgtgaa ataacatttc tctagaataa
    30961 tggcaaaaag ccagattatc ctttacagtc tttctatcta gtgtaacaaa aaagcagtaa
    31021 tttttcaact gctctttctt atttatttag cataatctac tgcacgaagc tcgcgaatca
    31081 cggttacctt gatatttcct ggataatcga gattgttttc aattttctta cgaactttgt
    31141 gagccaagat tgtgactttg tcgtccttga tttttcctgg attgaccatg atacgaattt
    31201 cacgtcctgc ttgaagggca aagctagttt gcactccttc aaagccgtta gcaatttctt
    31261 ccaaatcatg gagacgcttg atgtagcttt caagagactc actacgagca cctggacggg
    31321 ctgcgctcaa ggcatctgct gcagcgacga taactgctat cacgctctca gcttcaacat
    31381 ctccgtggtg actagcaatc gtattcacca caactggggg ttccttgtac ttacgggcca
    31441 attccatacc gatttcaacg tggctacctt caacctcatg gtcaatggct ttcccgatat
    31501 cgtgaaggaa tccagcacga cgggcaagag ccgcattttc accaagttcg ctcgccatga
    31561 taccagccaa cttagcaacc tcaatcgaat ggcgcaaaac attttgtcca tatgaagtac
    31621 ggaactgcaa acgtcccata atcttcatca agtctggatg aaggtttggc gcaccaattt
    31681 cataggcagc agcctcaccg tattcacgaa tcttattgtc aatctcttga cggtttttct
    31741 caaccaactc ttcgatacga gctggatgta tacgaccatc tttgagcaac atttccatag
    31801 tcatacgggc aatctcacga cgaatcggat caaatcctga caaggtcacc acttctggtg
    31861 tatcgtcgat aatcacatcg acccctgtca aactttcaaa ggtacgaatg ttacgacctt
    31921 cacgaccaat aatgcgtccc ttcatagtat cgtctggcag atgaactgtt gagtttgttg
    31981 actccgctac atattcacca gcgatacgtt gcatagcttg aaccaagatg tccttggcca
    32041 ttttgtcaga acgttccttg acctcttgct cagcttcgcg aatgcgactg gcaatctccc
    32101 tggtcaagtt ttcctctgtc tgagccaaga taatatctcg tgcttctgcc tgagacagag
    32161 caccaatacg ctctagttct gcttcttttt gtctttcgac ttcctctaat tgctcttcac
    32221 gcgcatcaag gtttttcgct ctatcagaaa tactttgttc tttttgttca agtgtttgtt
    32281 ctttactcgt caaattgtcg tccttacggt caaggctagt agctctctct gtcaaacgac
    32341 tttcgatttg tttgagttct tgacgttctg atttgaattc agcgtccact tcttcacggt
    32401 attttctggc ttcttctttg gcctccaata gtgcttcttt tttaagagac ttgctttcac
    32461 gtttggcttc attaacaagt aaatccgctt cacgctcagc ttgtccacgt aaattagttg
    32521 cttcttgttc agcatttaaa agcatcaact ccgcagcttc ctgagatgat ttcatcttag
    32581 ctgagatgct gacatatcca atgactaaac caatgatgac ggcaaaaaca gcaatcgcaa
    32641 gcgacatgat ctccatgttt ttacctcatt ttattgttat tccgaatgac atacattctt
    32701 ttacattcta ccataaaaaa gtgattttca caaacctaaa atagaatatg ttttggggaa
    32761 tttggaatat atttgccaaa ataaacttgt tttttagaaa tagtagttta gtagagactt
    32821 gagaaaaagc ctacctttca atagacttag taatgatctt taaaggacaa gaaagccacg
    32881 ctatctccat ccatcatata aatcaagcga ttttctgcat caatacgacg tgagcaagct
    32941 ccttggtagt catacttgag tggttctggt ttacctattc ctgtaaaggg atcacgttga
    33001 atatccttga ttagtttatt gattcttttt aacgttttct tatcctgatt ttgccagtag
    33061 caataatctg cccaggcatc ttctgtaaac ttgagcagca tttcttactc ctcaataaca
    33121 tggacctgag tacttccagc acgaacttga gccattcctc gcaaaacctt atcagaaagt
    33181 tccttatttt gagcaattct cagggtttct tggatactat cccactcact ctttgaaagg
    33241 actacaatgt cctcgtctgg atttttattg accaccgtca aaggctcaaa ttcatcattt
    33301 accttcttca tgtagtcctt taaatgattt cggaatgttg agtaaaggac tgcttccata
    33361 accatacctc gttttagctc ttttccacta ttatacacaa aaagaaagaa attgtcagga
    33421 acttgtacaa gattttcttt tctatctatt tatactcaat gaaaatcaaa gagcaaacta
    33481 ggaaactagc cgcaggctgt acttgagtac ggcaaggcga cgttgacgcg atttgaattt
    33541 gattttcgaa gagtattatt cgtaaaaaat ctcaaaaagc ctacctttcg gtagacttag
    33601 tttgtttcta ttctaatcgg cactcttcca aaattttgct ctgctatact tggcttccct
    33661 agttggtaaa tctggtctgc tttttgggtc atagcatccc aaggttcttt gccaattcgg
    33721 ctgactagac tgacctgtcc tttcagagac ttgagatgtt gcctgccttt ttcagtaaac
    33781 ccaaggacat gaatggcttc tggcaagaca ttttctctag cctgcaccaa gatataggac
    33841 aagaggcgtc tgacacgcgc cttggtgtaa cgtttggtag tcactaactc gaccaattct
    33901 tccacagact gggctgtttt aatagcttct ttaatgcgca cagccatttc ttgattgacc
    33961 tgatagatgg tggttaggtc gggatttgac aagatttgat agcggagcaa gggaaaatag
    34021 tcttcccaga tcaccttact ggcctgctca aatagggcaa cagaaggcat aaaacgttct
    34081 aagaaatctt ggtccttctg atgttgacgg agggctgtcg ccgaggcaaa gtccacatct
    34141 ttattcacag aatggtaacc tgccccctga cgctgaatcg gatacagctt gatattccgt
    34201 cctgcaaccg ccttggcata ggccagagca agaacatgat tgggggtatt acctgaaaaa
    34261 tcaagaccag caaattcctt ccacatagct tgggttttct ggggatagga aagggaatca
    34321 ggcagatttt tcacaaattt ctccatctca gcacctttct ctgtgtataa gtcagcgatt
    34381 ttctggtaat ccagaacttc ctctgtccca aaaactagac tatcaatgcc caagcgatcc
    34441 aagatatcca cagctccttg accaaagaaa tcagctgcct gaacactgac taaaaagggc
    34501 aattccacta ccaagtctgc gccattttcc agcgccatct gggtccttgt ccacttatcc
    34561 acaatagcag gctctccacg ctgcatgaaa ttccctgaca tggcaacaat tttcagtccc
    34621 tctgcctgat ccagcaggta tttatggcca ttatgaaaag gattgaactc cgcgataata
    34681 cctgtgatgg tcatgctaat ctcctacttc tgcgccacaa aaaaccaacg ggtgctagtt
    34741 tctgttggct ccttatcctc aaagtctgca tagagtttga aggacttaaa tccagcctgt
    34801 tccagcaaga tatcataggt caagatctca taggtcctct cctcatgtac ttcatcgtgg
    34861 cgactaaagg aaccgtcagc ctccttgata aagaaagtca gctcatgcac gatggagtga
    34921 ggaacttcgt cctcatatgt atcccaaagc atggcaaaat cttccgcatt ttcatggtag
    34981 gaatagccag ggaatacttc atctgtctgg taggtcgagt gcacgtcaaa gatgaaaact
    35041 ccctcttcat tcagcgcatt gtacacgtcc ttaaagacgt cccctacttc cacctcatcc
    35101 tgcatatagc agatagagtc cgaataacac gtgacaaaat cgtatttccc tgccttggac
    35161 aaattcagca tattaccttc tataaaagca atcttttgct tggctgaagt tgctctcttc
    35221 tccgcaatct tcaacatatc cgcactcaag tcaagtccag tcacatcaaa accagcctga
    35281 gagaagcgca ctgattgaat tcctgtccca caagccaatt ccaagagttt ctttctctcc
    35341 ttggtcttag gcaaatgacg cagagaaaaa ttcgtccatt tgtcgtataa actatcgtcc
    35401 atgaccgcat catagaccgc cgcaaaggtt tcataagtcg ccataatcat gatactaaga
    35461 gctccatggt aaacaccact agccctttac ctttctatca atttttctaa aataagcaaa
    35521 gaaacccagt tgactacaac tgagttcatc ttctaagcaa gagcttctga aatatctact
    35581 gaattcgcct catgccatag cttctctaga ttataatggg cacgcatttc ttctgaaaag
    35641 atatgcacga caacagcacc gaggtccagt aagacccagc ctccagctgc atcgccttcg
    35701 acatggctac ctttaaagcc tgcttgagct actttttcac ggatattagc tgcgatagcg
    35761 tccaactgac ggctattcat tgagctagtg atgacaaagt agtccgtcac actagtcaaa
    35821 tcttgtacat caagtgcgag gatatcctcc gcacgtttct catcagccgc tttcacgact
    35881 agttctagta attctttttc gttcatttaa tcctcttttt agaaaatatg tttgtaatct
    35941 agaacatctg caaagcgctc ctcccaaatg tcctcataaa attcattaat cgtttctgct
    36001 ggaatgtcat ccccatcaaa ggttgtgaca gcaccttctg gaacaataag ctgatagcca
    36061 tattcaaagg caaccttgac agaggtatcc acacaatatt ctgtctgcat accacataaa
    36121 actaattttt caatcccctg cttatccaag tattctttta agccagtttc tttgaaaata
    36181 ctgttatact tcttctgaaa gaccttttca gcaggttttc gatttaaaag cgcagataac
    36241 tgccaatctt ctgatgtttg agcttcagag ttctcaatat gttgaacata gataatttca
    36301 atattcttgc ttctagcctg gttttgtaaa taagaaattt tttccaatag actttttgtc
    36361 tggaacccag tttctactaa aacattctga acatcaatga ttataaaagc cgtcttcatt
    36421 tagtcctctt tcaaatagtg cacaaaggcg ttataggttt caagggtttg gggatagatg
    36481 gggaatccct gatgagctag atactccacg gtacgagctg tttcgtaggc caccgcctta
    36541 ttgagcgata gacttgcaat ctcacgcgcc acatctactc caggaaaggc tcgattgtgc
    36601 tcgatatagt ctgctacata gacaacctta tctaggtctg tcatctgacc agcaccgact
    36661 gtatggactt caatggctcg caggatttct gaatcatgca aatccaaatc ttcctgaatt
    36721 ttgtaaattc cgaccatacc atgccagaca ttattacccc agtttttgag gtcaggatct
    36781 agctggtaac ggtcaatcaa agctagaaat tcctgatctg acaacttttt agcatagtca
    36841 tgaagaagac ctgctagact tgctttctca acatctacac caaagcgctg agctaattcc
    36901 atagcggcac gctccacacc tagacaatgg gttagacgtt tctcaggtag aagctctgcc
    36961 attttttcca acaaggcctc acgggagcag ttgatatagt cttgatagga catcagtaga
    37021 gcccctcctt ctcgatgtag tctagcactg gctgaggtag gagaaagttg ggtttccgac
    37081 cttgggcaat gaagtcccgc accatgctgg acgagatatc catgagcggt acgtccaccc
    37141 agataactgg ataggaagtc cctgccttgt agcgtggacg ctgaaccccc acaaactgaa
    37201 ccatgtcaac cagttcatca attcggtacc acttaggcag atagtcaacc atgtcggcac
    37261 cgatgataaa gtaataatcc gtatctggat tcttctctgt caaaatcttc atggtgtcgt
    37321 aggtgtagga aatacccttg cgctccaact caatggtttc aatgactagg ccgtcaattc
    37381 cctcaattgc caactcaagc atcttgagac gatggtgttc agggatggtt tcctttttat
    37441 caacgtgagg aggttggtat tcaggcatga gaagaacttg atccagtccc aactgttgcc
    37501 gtacttgatc cgcaacaatg agatgggcat tgtgaacagg gttaaaattc ccccctaaaa
    37561 tcccaacttg tttgcgtttt ttctccttga tttctggctc caactctacc ttggtaaagg
    37621 gagtcaatag ttcgattgtc ataggctagt cccctttatt tctctataaa aacagttatt
    37681 tggagtttcg gtcttagatt tctttgactt tcttagaaat cttgcgattt tctttcttgc
    37741 tagattgttt aaacaaaatc aagatgcgtc ctattttttg gactgtatcc acaccgattt
    37801 cttcttccaa aatttcagct acttcgtgga tgttttcatc tgtgttttgt aagagagtaa
    37861 ccttgattaa ttcacgcgca tcaagagctt gacggacgct ggttttgatt tggtcgttga
    37921 gtccattttt cccgatttgg atgataggtt tgagggtgtg tgcctggctg ttgaggaagg
    37981 cacgttgttt tgatgttaat gacataattt ctttaaaaga gtttcttttt atacttttct
    38041 gaagtggtga cggacgtcag caaagtcctt cggactttca tgactaaaat ttgagcctaa
    38101 ggtctcaaat tttccgcagt tggtacaatc acttgtacca acttacacca cagcgaaaag
    38161 tattctctat ggggctcgcc ttgctcgccc aaattcagac aaccctttcc tttctgtgct
    38221 taaataattg cttttcgtgt gacgacggcg acgccttctg gtgcccagac ggcgactttt
    38281 gctgtgcctg ttacacgaat ccatcctagg cctgagatga ctaggtctgt cttatcttta
    38341 atggtaaaga catgctggac tagccttggg aaatcttctt tttccttgct atttggtggt
    38401 gtcagaagag ttcccaggtg cttatcgtag aaagcactag ctccttcaag cttgctacga
    38461 tggagtttga gttcattatc aaagaaagca gtaaatcctt gcttttctcc tgctataaag
    38521 tcaaagcgtc ccaaaccacc taaaaatagg gtttgctcag gattaagctg ataggtctta
    38581 ggcttgattt cctttttagg actgacatac ttgaggtttt tggccgtcaa gtagtgagcc
    38641 atctggtggc ggtggataat tcccggcgta tcgtaaatat aagatccgtc gtcaagcgga
    38701 atctctattt tgtccaaggt tgtccctgga aagcgtgaag tagtgatgac attctgatca
    38761 cccgtgattt cttggataat agcattgatt agagttgatt ttccaacgtt ggtcacaccg
    38821 accacataga catcgcggcc cttacggtag tgttcaatct tatcaatgac ttccttaatg
    38881 gcatgtttat tttgtgctga agttaggacc acatcgactg gacgaagacc ttcttcatgg
    38941 gcacgtttca tgagccactg gctaatctta ccagacttaa ctgacttagg aaggatatct
    39001 tttttatttc ctaccaagag gacatcattg cccgagacga aacgtggtaa acctgggatg
    39061 acagatccat taaaatcaaa gatatcaatg acattgacca ctaaagcatc actgtctccc
    39121 acctcgtgca agagcttgag gaaatcatcg ttcgtcaact ggacatctgt gatttcattg
    39181 tagtggcgga gacggaaaca gcgttggcaa tagacttcgc cagtctccaa acctttttca
    39241 agtgccgact ggggggtaaa accaagacca gccttatctg tcgtctgaat ggttgctcca
    39301 caaccaatac agagaatttc ttccatagtt aaattccttt tttatatgta atcggtccgt
    39361 acttttcagt gatttttctc ataacacgac gctcacgagt tcggttaatc tgcgttttga
    39421 ttgagtcatg ttggaccaag ggtttgacta aaattgaccg aatccctgca cggtgggctg
    39481 ctcgtatatc tgtcatgagc tggtcaccaa ccatgaccac ttcctttttg tcatagtgga
    39541 attccttcat agcacggtca ataccaaatg tgaagggctt caaggcccag taaacgtaat
    39601 caatcccaaa tttctcaact gctcgttgaa tgcgtttttt ggtgttattt gacactacga
    39661 taatgccaat acccgcgtcc cgaaggtcat gtagccattg cttcatctct ggcgttccat
    39721 cagggttgtt ccaagcaatg agggtattat ccaaatcgac caaaacagcc ttgattccct
    39781 gcgcctgcag gcttgggact gtcagatcat agactgcttc cacagcaaaa tctggtatat
    39841 aattttcaat cgccattctg gctccttttc ttaacttttt ttcgcaattt tccttagtaa
    39901 caaggataaa acaatccaca aacctgcact agccaaactg atatagagcc aagcatgggg
    39961 ctcatctgtt aaaggtaagg gaacattcat tccgaaaaag cctgtcacga ctgccaaaac
    40021 agctagcaag actgaaatga tagtcaaggt tgtcaaattg tcattcagat tattgtttag
    40081 aatattgttg taagaggctg aaagctgctg taaaatctga gagattaggt ctgtcatgga
    40141 taccagctga tgagcctcaa tcatggcatc atcaaactgt tctctctcaa tctcatcaaa
    40201 actacgatac aaggcatgac cttgaatatg ctctaacaaa atccgatttt gtttggcagc
    40261 tgccgtcaga taaaccatac cagtctccaa atcagacagg gcaaagaggt ttttcttggt
    40321 agttcgctgg cgcaagaggt cattgacctc atccctactc ttgtccatct gctcaatgac
    40381 aggatagtag gcattgctga tgatttccag actggcaaag agaaacttat aaatcgaaag
    40441 cgtgtcatgg ctctccagat aacgagtcat ctgttcaatg acataggcgt tcttggtatt
    40501 actaatggta atcaggcgac gatgctcgac aataaaggtc atgggaaaag tctcatagta
    40561 ggccttgtcc ttttttacgt ctaggacatt atagataaag gtaaccgtcc cactttcacg
    40621 gtggtagtcc atgtgggcgc gctcgtttct atccagtgcg tattcaatgg tttccttgtc
    40681 caaaccgtaa atttcagaaa ggtcttctag tttattcaac tttcctaggt ctaggtctat
    40741 ccaggtacaa ccattgccca actgcttttc taaaaccatc ttttctcctt attaaactcc
    40801 ctctattgta ccacaaatta cttaaaataa caggtctagt ctgtgcgtga gaaattgcta
    40861 acaactgtga tatttcggtt agggacaaag tgaagggctt gctcctcgct acgaagctgt
    40921 gtcgtacgaa ttcccacact gacaacctta cccgatacag taatcggtcc atttgtcaga
    40981 acgacctcat ctcccacatc cagttgacgt tcaaagagga tgaaaaagcc attgatgaca
    41041 tcagacagaa agccttgggc tcccatacca atcgctaccc cagcaatacc agctccagcc
    41101 agcaaactag aaactggcaa acctaaaatc gacaaaatgc agtagagtaa aaagaaatat
    41161 agcgtataat taaacacatt ttctagtaaa cgtgagatgg ttttttggcg tccaacatca
    41221 tgacgagaca tttttagaga aggtttgaca attctctgca ccatggtatg aagcattttt
    41281 ttagcaatat aaaatacaat caaaagcagt aaaagagaaa tgaccttggt tagaatattc
    41341 tcgataatgg ttgtcacatc tagcttttca atataagcct gaataaattt ttgcatataa
    41401 aactccttta tttcattata ccatattttc atcagtctca ttctccataa atattcaaaa
    41461 aatagacaga aaattcttga ttttgtctat catttatact ataatcataa tcactacaca
    41521 aaaaggagat tattatgaaa agaatcattc atgcttggaa taaggcaagc cttgtcaagc
    41581 gtatcttgat tggtatgctt tgtggggaat cctaggactg acccttccta atctctcagg
    41641 aattgggcta ctcggggatt tattcgttgg aggtctaaaa gctgttgctc ctatcctagt
    41701 ctttgccctc gttgccaatg ccctttccca acatcaaaag ggacaagata gcaatatgaa
    41761 aactgttgtt ttcttgtata tcctatgata gggactttcg ccgctgctct tgtagctgta
    41821 ctagcaagtt tcatcgtccc tattgaaatt accctaaata gtgccaatac tgaaattgca
    41881 ccaccagatg ggattgggca ggtcctcagc aacctcttgc tcaaactggt tgacagccca
    41941 gtcaacgccc tgcttactgc taactatatt ggaatcttat cttgggcagt catttttgga
    42001 atcgctatga gagaagccag taaaaccagt aaaaatagta aagaattgct aaaaactatc
    42061 gctgacgtga cttctaaaat tgtcgaatgg atcatcaatc tggctccatt tggaatcctt
    42121 ggtcttgttt ttaaaaccat ttctgacaag ggagtcggaa gccttgccaa ctacggtatt
    42181 ttattggttc cattagtaac gactatgctt tttgttgccc ctgtggtcaa ccctttgatt
    42241 gccttcttct ttatgagacg caatccttac tctctagttt ggaactgcct ccgtgtcagc
    42301 ggtgtgacag cctttttcac tcgtagttct gcgactaaca ttcctgtcaa catgaaactc
    42361 tgccatgacc ttggactcaa cccagatacc tattctgttt ctatcccact cggttctact
    42421 atcaatatgg ctggagtagc gattaccatt aaccttttga cccttgttac agttaacact
    42481 cttggaattc ctgttgactt tgccacagcc tttgtcctca gtgtggtagc agctatctca
    42541 gcctgtggtg cttcaggtat tgccggaggt tccctccttc ttatcccagt tgcttgtagc
    42601 cttttcggta tttctaacga tattgccata caagttgttg gggttggttt tgtgattggt
    42661 gtcatccaag actcatgtga aacagccctt aactcttcta cagatgtcct ctttaccgcc
    42721 gttgccgaat acgcagcaac ccgtaaaaaa taactcatca aggcaagcct gctcatgtct
    42781 tgtcttttac gcttttattc taacttatta ggaaattctt atgtctatta gccaacgtac
    42841 gaccaagctc atcttagcta cctgtcttgc ctgcctgctt gcttattttc tcaatctttc
    42901 gtcagcagtt tcagctggaa ttatcgctct cttgagccta tctgatacgc gtagaagtac
    42961 tttaaaactg gctcgcaatc gtcttttttc tatgcttcta gctctggcta tcagtgttct
    43021 agcttttcac ttgagcggat ttcatatctg gagtctcggc ctctatctgg ccttctacgt
    43081 tcctttagcc tacaagatgg gctgggaaat tggcatcaca ccaagcacgg ttttggttag
    43141 tcatctcttg gttcaagagt caacctctcc agaccttcta gtcaatgaat tccttctctt
    43201 tgctattggt acaggatttg ccttgcttgt taatctctat atgccttcac gagaagagga
    43261 aatccagcac taccacacgc tggtggaaga aaagttaaaa gatatcctcc agcgcttcaa
    43321 atactattta tccagaggag acggacgcaa ccgagcacag ctggtagcag aattagacac
    43381 gcttttgaaa gaagccctca gactggtcta tttggatcac tctgaccacc tctttcacca
    43441 gaccgactac catatccact actttgagat gagacaacga caaagtcgta tcctgagaaa
    43501 catggcccaa cagattaaca cttgtcacct tgccgccagt gaaagcctga ttttagcgca
    43561 gctcttttca aaaattgctg gtcaactgag ccagaccaat cctgcttctg atttgctaga
    43621 tgaaattgaa cgttatctgg aagtcttccg gaaccgcagt ctgcccaaga caagagaaga
    43681 gtttgaaacc cgcgccaccc ttcttcaact cctacgtgaa gccaaaacct tcatccaagt
    43741 aaaagttgat ttttaccaaa aatatggaca gtaaaaaata aaaccactaa aaaacagagc
    43801 ttgccagcat tcttgaaaag tagtataatt attgcatgcg caatcatctt gtgacgcaga
    43861 gactgcccgt taatggactt tcttctattt tatgactcta aaaagaaagg agacactatg
    43921 acctatttgg aaaaatggtt tgacttcaat cggcgtcaga aagaaattga aagtctcttg
    43981 gaagagacca ttgcccaaca gagcgagcaa agtctgacct tgaaagagtt ctacctgctc
    44041 tactatctgg acttggctga agaaaaatct ctacgccaga ttgacctgcc agataagctt
    44101 catctgagcc cgagcgcagt ttctaggatg gtggctcgct tggaagctaa aaattgcggt
    44161 ctactcagtc gcatgtgttg ccatcaagat agacgctcta gttttatctg cctgacaaat
    44221 gatgggcaaa agacactggc ctcgctacaa aagactgtcg aagaaagctt ggaagcaggt
    44281 ttggatttct tgatttaaga tgattttaaa aaaagttgcg tgcgcaatca tttttcttga
    44341 cattctcttt tataaggagt aaaataaagt tagcattaaa caaaggagtt ttaaatatga
    44401 ttgaaattac ctatatagat gccagcaaga acgaaagaac tgtgactttc gagtcttatg
    44461 aagactttga acgttcgcaa caagcttgcc ttatcggcgt cgcagactac taccctgtcc
    44521 aaaaattaac ttacaagggt cataatttgg actaccatgg gacttatgga gatatcttct
    44581 tctatctcat gaaacaagat ttaagccaat ataactaaaa aaaggagaaa tacaatggca
    44641 aaagcaatta cagatgcaac attcgaacaa gaaacaaaag acggtttggt cttagtagac
    44701 ttctgggcaa cttggtgtgg tccatgtcgt atgcaaggtc caatcttgga caaattgtct
    44761 gaagaacttt cagaagatgt cttgaaaatc gttaaaatgg acgttgatga aaatccaaac
    44821 acagctcgtg cttttggaat catgtctatt ccaactcttc tcttcaaaaa agacggccaa
    44881 gttgtcaaac aggttgcagg tgttcacaca gcagaacaaa tcaaggccat cattgctgaa
    44941 ttgagctaat caaacgagag accaagtact ttctttggtc tcttttttct tgccctttgc
    45001 catttttcaa aaaatatgct agactatagt atattgaaac tagaatagta cacctctact
    45061 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttatttc
    45121 attttactat ataagtagaa tattacgatg tttgggatat gccatcgcta aaaaaacctc
    45181 gatttggtat tttttagctc cctaatggga gctttttgcg tgctctgaac atttcccatt
    45241 ttggaaggag tactataaaa cgtcaatcag ccttggtcgt ctttagtggc ggtcaagatt
    45301 caacaacctg ccttttctgg accatgcaac actatgaaac agtcgaagct gtcacctttg
    45361 cctacggcca acgtcatcac ctcgaaattc aaattgctag agaaatcgct aaggaacagg
    45421 gcattcgtca ccatatcctc gatatgtctc tgctgggaca aatcactgct caaccagact
    45481 ttgcgacgat tcatatttcc tacattcctg acaagctctg tgtcgagtca aaatccctca
    45541 aactatatct atttagctac cgaaatcacg gagatttcca cgaaaactgt atcaacacca
    45601 tcgggaaaga cttggtcaac ttgctagacc ctcgctattt agaagtctgg ggaaaattca
    45661 ctccgcgcgg tggcatttca atcgacccct actacaacta cggtaagcaa ggaactaagt
    45721 atgagggctt ggcagaacaa cgcctcttcc aacacgacct ttatccagag aaaattgaca
    45781 accgctaaac tcatactcaa tgaaaatcaa agagcaaact aggaagttag ccgcaggtta
    45841 ctcaaagcag tgtttgaggt tgtagataag actgacgaag tcagcttaaa acattgtttt
    45901 gaggttgtag ataagactga cgaagtcaga tcaaaacact gttttgaggt tgcagataga
    45961 actgacgaag tcagatcaaa acactgtttt gaggttgtgg atagaactga cgaagtcaga
    46021 t
//
LOCUS       DAVKJU010000008        44153 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVKJU010000008 DAVKJU010000000
VERSION     DAVKJU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 44153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.008"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            96..167
                     /locus_tag="VW191_000366"
     tRNA            96..167
                     /locus_tag="VW191_000366"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Glu,seq:ttc)
     gene            173..246
                     /locus_tag="VW191_000367"
     tRNA            173..246
                     /locus_tag="VW191_000367"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:207..209,aa:Asn,seq:gtt)
     gene            complement(287..829)
                     /locus_tag="VW191_000368"
     CDS             complement(287..829)
                     /locus_tag="VW191_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEU9623201.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSIDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            957..2568
                     /locus_tag="VW191_000369"
                     /pseudo
     CDS             957..2568
                     /locus_tag="VW191_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(2598..5150)
                     /locus_tag="VW191_000370"
     CDS             complement(2598..5150)
                     /locus_tag="VW191_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEU9623202.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTLKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(5212..6834)
                     /locus_tag="VW191_000371"
     CDS             complement(5212..6834)
                     /locus_tag="VW191_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9623203.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEDDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            7040..8065
                     /gene="trpS"
                     /locus_tag="VW191_000372"
     CDS             7040..8065
                     /gene="trpS"
                     /locus_tag="VW191_000372"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU9623204.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            8217..9695
                     /gene="guaB"
                     /locus_tag="VW191_000373"
     CDS             8217..9695
                     /gene="guaB"
                     /locus_tag="VW191_000373"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU9623205.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SDNLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(9745..10842)
                     /gene="recF"
                     /locus_tag="VW191_000374"
     CDS             complement(9745..10842)
                     /gene="recF"
                     /locus_tag="VW191_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU9623206.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(10845..11213)
                     /gene="yaaA"
                     /locus_tag="VW191_000375"
     CDS             complement(10845..11213)
                     /gene="yaaA"
                     /locus_tag="VW191_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEU9623207.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            11372..12622
                     /locus_tag="VW191_000376"
     CDS             11372..12622
                     /locus_tag="VW191_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9623208.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILDETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVWKNVGQSILELGYHYCSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            12619..13902
                     /locus_tag="VW191_000377"
     CDS             12619..13902
                     /locus_tag="VW191_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU9623209.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVILTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13944..14765
                     /locus_tag="VW191_000378"
     CDS             13944..14765
                     /locus_tag="VW191_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9623210.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            14776..15321
                     /gene="pgsA"
                     /locus_tag="VW191_000379"
     CDS             14776..15321
                     /gene="pgsA"
                     /locus_tag="VW191_000379"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU9623211.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            15318..16145
                     /locus_tag="VW191_000380"
     CDS             15318..16145
                     /locus_tag="VW191_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9623212.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            16130..16969
                     /locus_tag="VW191_000381"
     CDS             16130..16969
                     /locus_tag="VW191_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEU9623213.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16962..17756
                     /locus_tag="VW191_000382"
     CDS             16962..17756
                     /locus_tag="VW191_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9623214.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            17814..18632
                     /gene="mreC"
                     /locus_tag="VW191_000383"
     CDS             17814..18632
                     /gene="mreC"
                     /locus_tag="VW191_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU9623215.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRAYNENESLKKQIYQLEVKSNEAESLKTENEQLRQLL
                     GMKSKLQATKTLAADVIMRSPVSWKQELTLDVGKSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGTTTIYGIIVGYDKENEVLKISQLNSNSDISTGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            18632..19126
                     /gene="mreD"
                     /locus_tag="VW191_000384"
     CDS             18632..19126
                     /gene="mreD"
                     /locus_tag="VW191_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU9623216.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGIATLLFILLGAFLHKLNSVILLNRW
                     TRILAMIVMSFLFDMGTYLFALVVGLTLDSLPIFIVYSLVPSMILNLVWMLIFQFIFE
                     RYYL"
     gene            19220..20407
                     /locus_tag="VW191_000385"
     CDS             19220..20407
                     /locus_tag="VW191_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEU9623217.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLENEITELSKNIVSRND
                     SLEKQARSAQTNGAATSYINTIVNSKSITEAISRVAAMNEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLSDDAQALTTKQAELKAAELNLAAEKATAEDEKASLL
                     EKKAEAEVAAKVAAEAEAAYKAKQVSQQQTVVASGNTTFAAQVQAVASSESATYTPVA
                     VKQRPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVG
                     AIACWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTLGNHRGWFNPTTTSSGFVTYI
                     YAD"
     gene            20632..21411
                     /gene="rpsB"
                     /locus_tag="VW191_000386"
     CDS             20632..21411
                     /gene="rpsB"
                     /locus_tag="VW191_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU9623218.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAAAVEAEFAASEAQADSIEEIVEVVEGDNA"
     gene            21490..22530
                     /locus_tag="VW191_000387"
     CDS             21490..22530
                     /locus_tag="VW191_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEU9623219.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALTMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVIEGGDEALAKQISMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKSQLTDEVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            complement(22652..23578)
                     /gene="cysK"
                     /locus_tag="VW191_000388"
     CDS             complement(22652..23578)
                     /gene="cysK"
                     /locus_tag="VW191_000388"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU9623220.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKTANSNIQVYAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     IAKKLGTGKKVLALAPDNGERYLSTALYEFEV"
     gene            complement(23863..24498)
                     /locus_tag="VW191_000389"
     CDS             complement(23863..24498)
                     /locus_tag="VW191_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEU9623221.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            24554..25852
                     /locus_tag="VW191_000390"
     CDS             24554..25852
                     /locus_tag="VW191_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9623222.1"
                     /translation="MKVNLDYLGCLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            25849..26511
                     /locus_tag="VW191_000391"
     CDS             25849..26511
                     /locus_tag="VW191_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU9623223.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            26591..27139
                     /gene="raiA"
                     /locus_tag="VW191_000392"
     CDS             26591..27139
                     /gene="raiA"
                     /locus_tag="VW191_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU9623224.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            27275..29248
                     /locus_tag="VW191_000393"
     CDS             27275..29248
                     /locus_tag="VW191_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEU9623225.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            29245..29697
                     /gene="rplI"
                     /locus_tag="VW191_000394"
     CDS             29245..29697
                     /gene="rplI"
                     /locus_tag="VW191_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU9623226.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            29741..31093
                     /gene="dnaB"
                     /locus_tag="VW191_000395"
     CDS             29741..31093
                     /gene="dnaB"
                     /locus_tag="VW191_000395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU9623227.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDHGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            31095..31361
                     /locus_tag="VW191_000396"
     CDS             31095..31361
                     /locus_tag="VW191_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623228.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            31450..32796
                     /gene="cbpD"
                     /locus_tag="VW191_000397"
     CDS             31450..32796
                     /gene="cbpD"
                     /locus_tag="VW191_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEU9623229.1"
                     /translation="MKILPFIVRGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      32879..32981
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            33052..33342
                     /locus_tag="VW191_000398"
     CDS             33052..33342
                     /locus_tag="VW191_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEU9623230.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            33305..34063
                     /locus_tag="VW191_000399"
     CDS             33305..34063
                     /locus_tag="VW191_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623231.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            34102..35109
                     /locus_tag="VW191_000400"
     CDS             34102..35109
                     /locus_tag="VW191_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9623232.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            35109..35837
                     /locus_tag="VW191_000401"
     CDS             35109..35837
                     /locus_tag="VW191_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623233.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            35956..36414
                     /locus_tag="VW191_000402"
     CDS             35956..36414
                     /locus_tag="VW191_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEU9623234.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            36416..38848
                     /locus_tag="VW191_000403"
     CDS             36416..38848
                     /locus_tag="VW191_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9623235.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            39169..39822
                     /gene="rr06"
                     /locus_tag="VW191_000404"
     CDS             39169..39822
                     /gene="rr06"
                     /locus_tag="VW191_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEU9623236.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            39819..41159
                     /gene="hk06"
                     /locus_tag="VW191_000405"
     CDS             39819..41159
                     /gene="hk06"
                     /locus_tag="VW191_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEU9623237.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            41242..41769
                     /locus_tag="VW191_000406"
     CDS             41242..41769
                     /locus_tag="VW191_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEU9623238.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            41848..>44153
                     /locus_tag="VW191_000407"
     CDS             41848..>44153
                     /locus_tag="VW191_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEU9623239.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSKN
                     NLTVTSSGQDISKKYADEVESHLQSILKDVNKNLKKVQHTQNVDFNKKLSRIKTKYLY
                     GLKEKSEAELTLKTKETKEELTAAFEQFKKDTLKSGKKVAEAEKKAKAQKEEDRRNYP
                     TNTYKTIELEIAEAEVGVAKAELELEFAQAQVQIPQDTEKINAAKSKVEAAKSNVKKL
                     EKIKSDIEKTYLYKLDNSTKETPKPRVRRNSPEIKAKGRVKNYEEANIELSKYMTDLY
                     KLDNSTKETPKSRVRRNSPQVGDSRELKETIDKAKETLSTYMVTRLTKLDPSVFWFAD
                     LLMDAKKVVEEYKTKLEDASDKKSVEDLRKEAEGKIESLIVTHQNREKENQPAPQPGG
                     QAGGSMVVPPVTQTPPSTSQSPGQKATEAEKKKLQDLIRQFQEALNKLDDETKTVPDG
                     AKLTGEAGKAYNETRTYAKEVVDKSKKLLSQTAVTMDELAMQLTKLNDAMSKLKEAKA
                     KLVPEVKPQPENPEPKPQPEGEKPSVPDINQEKEKAKLAIATYMSKILDDIKKHHLKK
                     EKHHQIVALIKDLDKLKKQALSEIDNVNTKVEIENTVHKVFADMDTVVTKFQKGLIQN
                     TPQVPEAPKSPEVPKVSDTPKAPDTPQVPEAPKSPEVPKVPEAPKAPDTPQVPEAPKS
                     PEVPKVPDTPKAPDTPQVPEAPKAPDTPQIPEAPAPETPAPAPEAPKTGWKQENGMWY
                     FYNTDGSMATGWLEYNGSWYYLNANGAMA"
BASE COUNT        14060 a         7191 c         9503 g        13399 t
ORIGIN      
        1 ttagaaaatt aaaagatatt ttagagaaaa aatctcaaaa gtgattgaca attagcaaga
       61 aattgatata atggttatat ctgctacaga tagaaggtcc gttggtcaag gggttaagac
      121 accgcctttt cacggcggta acacgggttc gaatcccgta cggactattt tatccgccat
      181 agctcagttg gtagtagcgc atgactgtta atcatgatgt cgtaggttcg agtcctactg
      241 gcggagtata gataaaaggg aacacagctg tgttcctctt tttgtatcaa tttgtatcac
      301 caagcatttt cataaggaag tctgttattt cttgagaact ttcttttttt ccatgtgcaa
      361 tccaagtttg gcagacacca aaaagtgcat gagttagata gatgctacta tattctaatt
      421 cagtggtatt tagattcagt tgcataaatc gcttttgtaa atctatactg agcatgatat
      481 gaagtttatt tcgtaagaaa ttttggattt ctttagtccc attttcagaa agaagggcag
      541 ccagaagtgg ttctgactct agatattcaa agacttctaa aatagcgtct cttttgtgat
      601 gagcatgttt ttgaaaaata tattcaaatg tatggaatag tttgctttga tagtgctcaa
      661 tcatatcata cttatcctta tagtgagtat agaagctgga acgactaatt ccggcttttt
      721 ctactaattt gacagtagaa attttatcaa atggctgttc catcagtaat tgtaccatag
      781 cattttcaat agttcgcttt gtttttaagc gtttgttact ttcttgcata tttcttcctt
      841 gtaaacaaat tagactatat gtctaaaaat agatttttta tcttgtaatt tagatttttt
      901 aatgtataat ctattatatc aaaattttag acaatatgtt taaaaaagga gaaactatgt
      961 ttaaagaatg gaaagcaatt taaaaaaaca acctttatta ttgtcatgat cgggatttct
     1021 cttattccag ctctgtacaa tatcatattt ttgtcattaa tgtgggatcc atatgggcaa
     1081 ttgtctgact tacctgtggc agttgtaaat aatgataaag aggcttccta taatggtaat
     1141 actatggcaa taggaaaaga catggtgtcc aatttaaaag aaaataaaac cttggatttt
     1201 cattttgtag atgaagagga aggaaagaag ggattggaag atggcgatta ctatatggta
     1261 gtgactttac caagtgattt atctaaaaaa acaactacat tatccaatat tcaatcgaca
     1321 gcagcttatc aatcattgac aagtgagcaa caaactggga taagtgattc tgtatctcaa
     1381 aattcaactg atagtattca atcggctcag tcaattgtag ctttagtaca aggtttacag
     1441 ggaagtttag aaaacttaca aaatcaatct tctaatcttt cgactttaaa aaatcaagct
     1501 aatcaagtat tacctattac ttctacttct ttgataggat tgtcaagtgg attaacagag
     1561 atacaaggag ctgttactag caaattagtt cctgccagtc agttgattgc atcaggtgta
     1621 aacgcatata ctacaggtgt tgataaagtt tctcagggcg caagtcaact aagtgaaaaa
     1681 aatgccacct tgacacaaaa atcttctaga ttgacagcag gagttgataa attacaatca
     1741 ggatctgggc aattagcaga caaatccagt cagttacttt caggtgcttc ttcattagag
     1801 aatggagcta ataaattggc agatggatct gggaaactag cagaaggtgg aacaaagtta
     1861 acttctggat tggaagattt acagacagga cttgcttctt taggacaagg actaggtaat
     1921 gctagtgatc aactcaaatc agtatcaaca gaatctaaaa atgcagagat tttgtcaaat
     1981 ccactcaatc tttcaaaaag cagacaatga tcaagttctt gtaaatggaa tcgcaatagc
     2041 tccttatatg atatcagttg ctctttttgt tgcagcaata tcaacaaata tgatattttc
     2101 gaaattgcct tcaggacgtc atccagagat ccgttgggct tggttgaaat ctcgagctga
     2161 aataaatggt attatagctg ttttggcagg aattttggta tatggaggag ttcatcttat
     2221 tggtttaact gctaatcatg agatgagaat atttattctc atcatcctaa caagtttagt
     2281 attcatgtct atggtgacca ctttaacaac gtggaatagc cgtataggag ctttcttctc
     2341 acttattttg cttttactac agttagcatc aagtgcaggt acttatccac ttgctttgac
     2401 aaatgatttc tttaaatcta ttaatccctg gttaccaatg agctattcag tttcgggatt
     2461 acgacaaaca atctccatca acaagtcatt ttcctagctg tcatactagc tctatttact
     2521 ggtttaggta tactagccta tcaacataag aaaatggaag aagattaaaa aaatcgaccg
     2581 attaactggt cgatttttta tgccttagat gactttcgtc tgtgattata gattccaaat
     2641 agtaagagag aagtaaagga acagattgct ccagtaataa aaccattggg aatgaaggaa
     2701 agtgtaatag ttcctttccc cttgggaatg tcaactttca taaatccagt ttgagcttgt
     2761 ttaatttcta ttttcttacc atcttggtag gcagaccaac ctttgtcata aggaatggtg
     2821 aagaaaatag atgtatcttg ttggacatca tatgtagcaa aaaccttgtt tttagaagtt
     2881 gatactgtga caggttgttc tttaattttt tgaattgcct cggtgaaagt tttggtatct
     2941 aaacgataga aggtaggaga ttcaaatgat acttgtgaat ttccagggaa actaacattg
     3001 atattgaaag tttttttctc tttagtatat cctagattaa agaaggagaa gacattatca
     3061 gttgtaaaag tctttttttc accatttaca aggatgtcaa ccttcttttg tttatcgtta
     3121 gaaaagtgaa ggtttatgaa agagagataa acttggctgt tttctggaac ttcaatttga
     3181 tactggattg ctgcatcttc atttgaagaa cttgtgacac taatcaaatc attagtattt
     3241 tctatttttt ctgttttttc ataaggtatt ggagaaaaat aatcaaaatt gacgttagca
     3301 agttgattta aaaatgaggc ctgattatcc aaggtatgtt cattgaactt gacatcattg
     3361 taaacagatt gactagcaac tgcaatcgga agagagtatt gattttcata tagggtaaga
     3421 ttatcttttt gatagatatc tttaaagcca tacttatcaa taggactgtc tgagatattg
     3481 tactggatac caaataaact atcagccaaa atactattat ttgcatatcg gagattgaga
     3541 ttagtcccag aggatttaaa accaagttta tctaaagtag agcttgatga acgatttcga
     3601 acagatgaaa attgagagat tccattgtag ttgaatttca tactgtcatt tcctgtctga
     3661 gtttgtagtt tttcagtacg agtaaattga tttccaatat atgttgagaa agattccata
     3721 gctgggatat ctcgactata agcacttcga gaagcaaatc cccattcctt agcaattccg
     3781 tccatttgag atgaagcatt taaactcatt tcaaccagta taaataaaga gattagaatg
     3841 gcaaatagat tcacagatat aaactttttg ataactgcaa ggagtaaaag agaatagaca
     3901 accaaaaatt caagagtaag cagaatattc aaatctgtta aaaaagaata atgcgatttt
     3961 agatagatgg tagctaaaaa tcctgttact acaagaaaaa gcgaaactaa aaaattccag
     4021 actttaagtt ctttcagacg ctttaagact tctgctgctg tgtaaattaa caaggtagag
     4081 aaaatccaag catagcgatg taaaaacatg tttggagtat gcatgccttg ccaaaataag
     4141 tcaagagctt ctatgtaaaa gcttgcaatt agaaatgcaa agaatattac atatatgagt
     4201 ttcacgtgaa acttaataga tttcagcgta aaaaataaaa tggtcaaaat aaagggaaat
     4261 agtccaacaa aaatcattgg gatggcccca tactttgttg tgtcaaagga accaatgaat
     4321 tgcttagcaa agagatcaag ataccagcta ctttcagttt gaaactttgt aacttcagtc
     4381 aatttttccc catgtgtctg taaatcaaat agagtgggaa gagtcataat caaactagcc
     4441 ataccagcta aaaaggagat aactatgaaa tcaagaacag atgattttag agtcttaaag
     4501 tcccacgaaa tttgacagag ataccagaaa ataagaaaca atactgtcat atatccaaaa
     4561 taataatttt gaataaataa gattgacaga cttgtaaagt acaataggag tttcttttca
     4621 gttatcagta gatgtaaacc agttataatt aaaggaatca agataaaaac atctagccag
     4681 gtttttatct ctaattgact gacagtgaaa ctcatcagag cataggaagt agataaggct
     4741 agttttaaag tctgagggat agattgaaac aatttattca aactaaaaaa ggttgacaga
     4801 ccaatcaatc caaattttaa gagagtggtc agatagacag catctggcat attcgttaga
     4861 tcaaaaaagt aaactagagg cgcgagaaaa ctacccaagt aataactaga tagggcatag
     4921 aagtttagcc ctagaccact tgtaaaggtg taaaacagac tactatttcc atgtaggata
     4981 tttcgtaagg ctacatcaaa aataacgtat tgatgaaagc catctcctaa tagaggagag
     5041 ttgtcgctat tccagtagat accttgagat agatatactc cagacataat cactacagga
     5101 atgatgaaag aaataaaata ggttcgatat gtttttaaaa atgatttcat gttacctcgt
     5161 agaatgatag aaaactcagt tggttaaccc aactgagttt tgaagtttta tttagtcttt
     5221 ccaaagttct ttaacttttg cttgtacttc tgcattttct aggaattcat cgtaggtttc
     5281 atcgatacgg tcaatgacgc catttttaga taagacaatg atatggttag ccaaagtttg
     5341 aataaactcg tggtcatggc tggcaaagat gattgattct ttaaagtttt tcaatccatc
     5401 attcaagctt gagatagatt ccaagtccaa gtgatttgtt ggatcatcaa gtacaaggac
     5461 atttgatttt aagagcatga gttttgaaag catgacacga actttttctc cccctgacaa
     5521 gacatttaca ggtttgttaa cttcatctcc agagaagagc atacggccga ggaaaccacg
     5581 taggaaagta ttgtcatcat cttctttact tgcgaattga cgcaaccagt caagaattga
     5641 ttctcctcct gcaaaatcag ctgagttatc ttttggtagg taagattgac tagttgtaac
     5701 tccccacttg acagttcctt catagtcaat atctcccatg attgcacgaa ttaatgcagt
     5761 cgtttgaata tcattttgtc caataagtgc tgtcttatca tctggacgca agatgaaact
     5821 aatattatcc aagatagttt caccatcaat ctttacagtt agattttcta ctgtcaagag
     5881 atcattacca atctcacgtt ccgctttaaa gttgataaat ggatatttac gactagatgg
     5941 tacaatctct tctagctcaa tcttatcaag cattttctta cgtgatgttg cctgccttga
     6001 cttagaagca ttggcagaga aacgagcaac gaattcttgc aattgtttaa ttttttcttc
     6061 tgctttagca ttacggtctg ctagcaattt agcagcaagc tcagaagatt ccttccagaa
     6121 gtcgtagttt ccgacataga gtttgatttt tccaaagtca aggtcggcca tgtgagtaca
     6181 aactttgttt aagaagtgac ggtcgtggga tactacgata actgtgttat caaagtcaat
     6241 taagaagtct tctaaccaag taatcgattg gatatccaaa ccgttagtag gctcgtccaa
     6301 gagaagaaca tctggtttac caaaaagtgc tttggcgagg agaaccttta ctttttcacc
     6361 gttggccaat tcgctcatgt tttggtagtg taattcttct ggaatgttta ggttttgaag
     6421 tagttgagag gcttcactct ctgcttccca acctccaagt tcggcaaact ctccttcgag
     6481 ttcggcagca cgaaccccgt cctcgtctga gaaatcttcc ttcatgtaga tagcatcttt
     6541 ctctttcatg atgctataaa gtttttcatt tcccatgata acgacatcaa tggcacgttc
     6601 atcttcgtag tcaaagtgat tttgacgaag aacagagaga cgttcatctg gaccaagaga
     6661 gatgtgacca gtagtaggtt cgatatctcc agctaaaatt tttaaaaagg ttgattttcc
     6721 ggcaccatta gcaccgatta atccgtaagt atttccttct gtaaatttga tattgacatc
     6781 atcaaaaagt ttgcgatcac taaaacgtag tgaaacatca gatactgtaa gcaatatttt
     6841 tctcctatat gtgtaatata tttattctac tagaaaatac agaaatattc aaatttttat
     6901 ttgtcaattt tgtgtaaatt atatttacag tatcctttac acaaatctgt aaaaagcaag
     6961 gctgatttat tttgataaat tacggttatt tcattaaaaa aatgctataa ttgaaaggac
     7021 tatatcgaag gagaacaaaa tgactaaacc cattatttta acaggagacc gtccaacagg
     7081 aaaattgcat attggacatt atgttggaag tctcaaaaat cgagtattat tacaggaaga
     7141 ggataagtat gatatgtttg tgttcttggc tgaccaacaa gccttgacag atcatgccaa
     7201 agatcctcaa accattgtag agtctatcgg aaatgtggct ttggattatc ttgcagttgg
     7261 attggatcca aataagtcaa ctatttttat tcaaagccag attccagagt tggctgagtt
     7321 gtctatgtat tatatgaatc tagtttcgtt agcacgtttg gagcgaaatc caacagtcaa
     7381 gacagaaatt tctcagaaag gatttggaga aagcattccg acaggattct tggtctatcc
     7441 aatcgctcaa gcagctgata tcacagcttt caaggctaat tatgttcctg ttgggacaga
     7501 tcagaaacca atgattgagc aaactcgtga aattgttcgt tcttttaaca atgcatataa
     7561 ctgtgatgtc ttggtagagc cggaaggtat ttatccagaa aatgagagag cagggcgttt
     7621 gcctggttta gatggaaatg ctaaaatgtc taaatcacta aataatggta tttatttagc
     7681 tgatgatgcg gatactttgc gtaaaaaagt aatgagtatg tatacagatc cagatcatat
     7741 ccgcgttgag gatccaggta agattgaggg aaatatggtt ttccattatc tagatgtttt
     7801 tggtcgtcca gaagatgctc aagaaattgc tgatatgaaa gaacgttatc aacgaggtgg
     7861 tcttggtgat gtgaagacca agcgttatct acttgaaata ttagaacgtg aactgggtcc
     7921 tattcgtgag cgccgtattg aatttgctaa ggatatggga gaagtttata atatgattca
     7981 aaaaggtagt gaaagagcgc gtgaagttgc gggtcaaacc ctatctgagg taaaaggggc
     8041 aatgggactt cattacttta actaagttta ttttacaaga aactatcatt tctatctcga
     8101 agaaaagata gttttttatt tacccctgta acatttgaca aatttttata gaatggtatg
     8161 atagatacaa tataaaaaga gtcaagctca aaaagaaaga aaagaggaaa cttcgaatgt
     8221 ctaattggga cactaaattt ttgaaaaaag gttttacctt tgatgatgta ttgcttattc
     8281 cagctgaaag tcatgtgttg cctaacgatg cagatttaac aactaaattg gcagataatt
     8341 tgactttaaa tatcccaatt attaccgctg ccatggacac agttacagag agtcaaatgg
     8401 ccattgctat tgctcgtgca ggtggtctcg gagttatcca taaaaacatg tcaattgctc
     8461 aacaagcaga cgaggttcgt aaggtaaaac gttctgaaaa tggagttatt attgatccgt
     8521 tcttcttgac gcctgaacat acaattgctg aagcagatga gcttatgggt cgttaccgca
     8581 tcagtggtgt tccagttgtt gaaacacttg aaaatcgtaa attggttggt attttgacaa
     8641 accgagatct tcgttttatt tcagattata atcaaccaat ttcaaaccat atgactagtg
     8701 acaatcttgt tactgctcct gtgggtacgg atcttgcaac ggctgagagt attcttcaag
     8761 agcatcgtat tgaaaaactt ccgttggtcg atgaagaagg cagtctttct ggtttgatca
     8821 ctatcaaaga tattgaaaaa gttattgagt ttccaaatgc ggctaaagat gagtttggtc
     8881 gtcttctagt tgcaggtgca gtaggtgtta cttcagatac atttgaacgt gcagaggctc
     8941 tttttgaggc aggagcggat gcgattgtta ttgatactgc acatggtcat tctgcaggtg
     9001 tcttgcgtaa aattgccgag attcgtgctc atttcccaga tcggactttg attgctggaa
     9061 atattgctac tgctgaaggt gcacgtgccc tttatgaagc gggtgtagac gttgttaagg
     9121 ttggtattgg accaggttct atctgtacta ctcgtgtgat tgctggtgtt ggtgttccgc
     9181 aagtaacagc tatctacgat gctgcagctg ttgcgcgcga atatggtaaa acgattattg
     9241 ctgacggtgg gatcaagtat tctggagata ttgtaaaagc acttgctgca ggtggaaatg
     9301 ctgttatgct tggatctatg tttgctggaa ctgatgaagc tccaggcgaa actgaaatct
     9361 tccaaggacg taaattcaag acttaccgtg gtatgggatc aattgctgct atgaagaaag
     9421 gttcaagcga ccgttatttc caaggttctg tcaatgaagc aaacaagctt gttccagaag
     9481 gaattgaagg tcgtgttgct tataaaggag cggcagctga tattgttttc caaatgattg
     9541 gtggtattcg ctctggtatg ggttactgtg gtgcagctaa ccttaaagaa ctacacgata
     9601 atgctcaatt tattgaaatg tctggtgctg gtttgaaaga aagccatcct catgatgtgc
     9661 aaattactaa tgaggcacca aattattcta tgtaaaaaca atgaaaagaa ctccagtgaa
     9721 aacaggagtt cttttacaat gttgtcaatt tccatttaca gcagctttac catcctgaat
     9781 agtgaagata cttagatttt ctggcagatt ttgaagatgg tctaagcttg ttgttgtgat
     9841 aaaggtttgg attgattgag aaatcgtttc taataatttt aactgtctag tgttgtcaag
     9901 ttcactcatc acatcgtcaa gcagtaatat cggagattct gtggtaatgc tttccattaa
     9961 ttcgatttct gctaatttta tcgagaggac gagactacga tgttgacctt ggcttccgaa
    10021 actagcatcc atcccattta tataaaaaga aatgtcatct cgatgaggac cgacaccagt
    10081 attcttttta aataaatctc tggatctact tttttctaaa gcaattttga aagattcgga
    10141 taagttttgt ttgtcagtta tattgacaga agattgatag gatattgaca actcttcgat
    10201 ctgattagag agttcaaaat gtttcttacg cccaaatgat tctagttttt ttatgaaatc
    10261 taagcggtga ttcattacac gacatccata atcaactagc tgatcatcta acacagaaag
    10321 gaatgtttca tctatttttt gagctgattt taggtaagtg tttctttgct ttaggatgtg
    10381 gttataattg gttaagtcag ataaatagat tggcttaatt tgcccaagtt ccatatcaat
    10441 gaattttcgt cgaatcgaag gtgctccttt aattagttgt aaatcttcag gagcaaataa
    10501 gacaacattc atgtgtccta cataatctga aaggcgtgcc tgttttaagt gattaacttt
    10561 tgttacacgg cctttttgtg ttagttcgat ttctagggga atggatccag tttttttctg
    10621 aacgagacct gaaagatgaa gttgttcctc atcaaaatga atgagatttt tatctgttcg
    10681 agttcgatga ctacgcgtta aggctaaaaa atagatagcc tctaacatgt ttgtttttcc
    10741 ttgtgcatta cgtcccaaaa agacattcaa tttaggatta aagtctattt tcgtttcttt
    10801 gtaattacga aaagtcttga gagatagatg ttgtagccac atgattatct accagggaat
    10861 cgtggagctt gttggctttt aggtgatgaa gcaggtttcg atttgtcttt tttaactccc
    10921 ttattcatct ttttgacaag tttagcgatc cgttcttttt caactttatc agcttggtat
    10981 tcatcttgtt cttcagaagt aggttgtgtc aacaagatgt caatgttcat gtcagggatg
    11041 tcaactttat cgccaatacg aagttttttt ccacgacgac tctctaattc cctattaaag
    11101 taaacagaat gttcagagag aaatgattta atagctcctc cgctatgtgt aattccaagt
    11161 tctttgagta gtgcttggag ggtaataaat tcttcaaata atttgtattc cataattcac
    11221 ctcaatcttt ggtattatac catattttcc taaaaatagt gagagagaca gcgagaaatg
    11281 taagcgattt ttatttcaaa agtgttacag ggaacaagaa aatttcaggt tttcgtgata
    11341 taatagaagt ctgtatataa ggaggtaaat catggagtta gtgcatggaa tttcaacaca
    11401 ttttatccaa tcaaaaaagt ttaaaacaaa caaaattacc gtgcgtttta ccgctccatt
    11461 atcccttgat acgattgcag gtcacatgtt gagtgcaagt atgctagaga ctgctaatca
    11521 gatgtacccc acttctcaag atttgaggag acacttggcc agtctatacg gtacagatat
    11581 gtcaaccaat tgtttcagaa gagggcaaag ccacattata gaattgacat ttacctatgt
    11641 tcgtgatgag tttttaagta ggaaaaacgt gctaacttct cagattttgg aacttgtaaa
    11701 agaaactctt ttttcacccg tagtagttga taatgggttt gatccggcct tatttgaaat
    11761 tgagaaaaaa caattgctag caagtttagc agctgatatg gatgattctt tttattttgc
    11821 acataaagaa ttggataaat tgttttttca tgatgaacgt cttcaattgg aatatagtga
    11881 tttacgaaat cgtattttag atgaaactcc acaaagttct tattcttgtt tccaagaatt
    11941 tttagccaat gatcgaatag atttcttttt cctaggtgat tttaatgagg ttgaaattca
    12001 aaatgtatta gaatcatttg gctttaaagg tcgaaaagga gatgtgaagg ttcagtattg
    12061 tcaaccttat tctaatatcc ttcaggaagg tatggtttgg aaaaatgtgg gacaatccat
    12121 tttggaatta ggttatcatt actgttctaa atatggtgat gagcaacatt tacccatgat
    12181 tgtaatgaat ggtttacttg gtggatttgc tcactctaag ctctttacaa atgtccgtga
    12241 aaatgctgga ttagcttata ccatttcaag tgagcttgat ttatttagtg gattcttgag
    12301 gatgtatgct ggtatcaatc gagaaaatcg taaccaggct cgtaaaatga tgaataatca
    12361 actgcttgat ttaaaaaaag gttattttac agagtttgag ttaaatcaga ccaaggaaat
    12421 gattcgttgg tcgttgttac tttctcaaga taatcaatct tcattgattg aacgtgctta
    12481 tcaaaatgcc ttatttggaa aatcttcagc agactttaaa agttggattg caaagcttga
    12541 acaaattgac aaagatgcta tttgtagagt agctaataat gtgaaactac aagcgattta
    12601 ctttatggaa ggaatagaat gacaaaggtt gtttttgaag aaaaatacta tccagctgta
    12661 aaagaaaagg tttatcgaac tcgtttggcc aacggattga cagttgctct tttgcctaaa
    12721 aaggaattta aagaggttta cgggagtgtc actgtacagt ttggttcggt agatacgttt
    12781 gtcacagaag ttgacggaga tgtaaaacaa tatcctggag gaattgctca ttttcttgaa
    12841 cataaattat ttgagagaga agattctagt gatttgatgt cggcttttac gagtctaggt
    12901 gcagatagta atgcctttac aagctttaca aaaacaaact atcttttttc agcaacggat
    12961 tattttttag aaaatttaga tttacttgat gaattggtaa catcagcaca ctttactgaa
    13021 gcttccattc tgacagagca ggatattatt cagcaagaac gagaaatgta ccaagatgat
    13081 ccagattcgt gtttattctt ttcaacttta gcgaatttgt atcctggtac acctttagca
    13141 actgatatag ttggaagtga ggagtccatt tcccaaatca atctaactaa tttgcaagaa
    13201 aattttacaa agttttacaa acctgtaaac atgtctctgt ttttagttgg taattttgat
    13261 gtggagcgag tacaggacta ttttgaaagc aaagaactga aagattcaga ttttcaggaa
    13321 gtagcaagag aaaagttgtt tttacagcct gtaaagccaa cagatagtat gagaatggaa
    13381 gtatcttctc ccaaactagc gattggagtt agaggtaagc gagaagtttc tgaagcggat
    13441 tgctatcgac atcatatttt attaaaatta ttgtttgcaa tgatgtttgg ttggacttcg
    13501 gatcgttttc aaaaatgtta tgaatcaggt aaaattgatg catccttatc tctggaagtt
    13561 gaagtaacaa gtcgctttca ttttgtcata ttgacaatgg atacgaaaga gccagttgct
    13621 ttgtctcatc aatttaggaa ggctattcgt aattttacaa aggatttaga tattacagag
    13681 gaacatttag atattatcaa aagagagatg tttggcgaat ttttcagtag catgaactct
    13741 cttgaattta ttgcaacgca atatgatgct tttgaaaatg gtgagacaat ttttgatttg
    13801 ccgaaaattt tacaggaaat tactttagag gatgtccttg atgctggaca tcatttaata
    13861 gatgatggtg acatagttga ttttacaata ttcccatcgt agtaacctat tataatagac
    13921 actagaaaga agggatgaca agtatgagaa aaaaaacaat tggagaggtt ttacgattag
    13981 ctagaatcaa tcagggattg agtttagatg aattgcagaa aaagacagaa atccagttag
    14041 atatgttgga agcaatggaa gcagacgatt tcgatcaact tccaagtcct ttttacacgc
    14101 gttctttctt gaaaaaatat gcatgggctg ttgagttaga tgaccaaatt gttttggatg
    14161 cttatgattc tgggagtatg attacttatg aggaagtaga tgttgatgaa gatgagttga
    14221 caggtcgtag acgttcaagt aagaaaaaga agaaaaaaac atcattttta cctttatttt
    14281 attttatcct ttttgcttta tcgattttaa tttttgtgac ttattatgtt tggaactata
    14341 ttcaaactca accagaggag ccttctcttt ctaattacag tgtggttcaa tcaacaagtt
    14401 caactagctc tgttccccac tcctcaagta gtagttcttc tagtatagaa tcagctataa
    14461 gtgtatcagg cgaaggaaat catgtagaaa tcgcttataa gacaagtaag gaaacagtta
    14521 aattgcaatt ggcagtttca gatgttacaa gttgggtcag tgtttcagaa agcgaacttg
    14581 agggcggtgt aaccttatcg ccaaagaaga aaagtgcaga agcaacagtt gcaactaaaa
    14641 gtcctgtaac aattacgtta ggtgttgtaa aaggtgttga tttgacagta gataatcaga
    14701 ctgttgattt atcgaaatta acagctcaga ctggacaaat cactgtaacc tttactaaaa
    14761 attaaggaaa aacgaatgaa aaaagaacaa attcccaatc tcttaacaat aggtcgaatt
    14821 ctctttatac ctatttttat ctttatttta acgataggaa attcgataga gagtcatata
    14881 gttgcagcta ttatctttgc tgttgccagt attaccgact atttagatgg atatttagct
    14941 cgtaaatgga atgtggtcag taattttggt aaatttgcag atcctatggc ggataagtta
    15001 ctagttatgt cggcttttat tatgttgatt gagttaggta tggctccggc ttggattgtt
    15061 gcagtgatta tctgtcgtga gttagctgtg acaggtttaa ggcttttatt ggttgaaact
    15121 ggtggaacaa ttttagcagc agcaatgcct ggaaaaatta aaacttttag tcagatgttt
    15181 gctattattt tcttgctatt acattggact ttgcttggtc aagttctact ttatgtagcc
    15241 ttatttttca ctatctactc tggctatgac tatttcaagg gtagtgccta tgtatttaaa
    15301 gggacatttg gttcgaaatg aaatcaataa ttgatgtaaa aaatctttct tttcgctata
    15361 aagaaaatca gaactactac gatgtgaagg atattacgtt tcacgtgaaa cgtggagaat
    15421 ggctttcgat tgtagggcat aatggtagtg gtaaatcaac gacggttcga ttaattgatg
    15481 gcttactgga agcagaatcc ggagagattg taattgatgg ccaacgtttg actgaggaaa
    15541 atgtttggaa tatacgtcgt caaatcggta tggtttttca aaatccagac aatcaatttg
    15601 ttggagcgac tgttgaagat gatgttgctt ttggtttgga aaatcaggga ctttctcgtc
    15661 aagaaatgaa aaagagagtg gaagaagctc tggctttagt tggcatgttg gactttaaaa
    15721 agagagagcc agcgcgtcta tcaggtggcc aaaagcaacg tgtggccatt gcaggtgttg
    15781 tagccctaag accagctatt ttaatcttag atgaagcaac gagtatgttg gatcctgagg
    15841 ggcgtagaga acttattggg acagtaaaag gaattcgaaa agactatgat atgacggtca
    15901 tttctattac ccatgatttg gaagaagtcg ccatgagtga tcgcgtattg gtcatgaaaa
    15961 aaggggaaat tgaatcaact agtagtccaa gggagctttt ctctcgaaat gatttagatc
    16021 aaattggatt agacgatcct tttgctaatc aattaaaaaa atctttgagc cagaatggct
    16081 atgatttacc tgaaaattat ttgacagaaa gtgagctaga ggataagcta tgggaattgc
    16141 tctagaaaat gtgaatttta catatcaaga aggtactccc ttagcttcag cagctttgtc
    16201 ggatgtttct ttgacgattg aagatggctc ttatacagct ttaattgggc acacaggtag
    16261 tggtaaatca actattttac aactcttaaa tggtttattg gtgccaagtc aagggagtgt
    16321 gagggttttt gataccttaa tcacctcgac ttctaaaaat aaagatattc gtcaaattag
    16381 aaaacaggtt ggcttggtat ttcagtttgc tgaaaatcag atttttgaag aaacggtttt
    16441 gaaggacgtt gcttttggac cgcaaaattt tggagtttct gaagaagatg ctgtgaagac
    16501 tgcgcgtgag aaactggctc tggttggaat tgatgaatca ctttttgatc gtagtccgtt
    16561 tgagctgtca gggggacaaa tgagacgtgt tgccattgca ggcatacttg ccatggagcc
    16621 agctatatta gtcttagatg agccaacagc tggtctagat cctctaggga gaaaagagtt
    16681 gatgaccctg ttcaaaaaac tccaccagtc agggatgacc atcgtcttgg taacgcattt
    16741 gatggatgat gttgctgaat atgcgaatca agtctatgta atggaaaagg gacgtttagt
    16801 aaaggggggc aaaccaagtg atgtctttca agacgttgtt tttatggaag aagttcagtt
    16861 gggagtacct aaaattacgg ccttttgtaa acgattggct gatagaggcg tgtcatttaa
    16921 acgattaccg attaagatag aggagttcaa ggagtcgcta aatggatagt atgattttag
    16981 ggcgttatat cccaggggat tcgattgttc accgattgga tccacgtagc aaattgcttg
    17041 ctatgatgct actgattttg attgtgtttt gggctaataa ccccttgacg aatctgattc
    17101 tttttatagc gacagggata tttattgcct tgtcaggagt atctctttca ttttttattc
    17161 agggcttgaa gtctatgttt ttcttgattg ccttcacaac tatttttcaa ctatttttca
    17221 tttctaatgg gaatgtttta tttgagtttt cgtttgtgag aatcacggat tatgctttgc
    17281 aacaagctgg gattattttt tgtcgctttg tattgattat tttcttttca actttgttaa
    17341 ccttaacaac catgccttta agtttggcat cagctgtcga agctttatta gcacctttaa
    17401 agcgtgtgaa agttccagtt catgaaattg gattgatgct gtctatgagt ttgcgttttg
    17461 tcccaacctt gatggatgat acgactcgga ttatgaatgc acagaaagct cgtggagtgg
    17521 attttggaga aggaagcatc gttcaaaaag taaaggcgat gattcccatt ttgattcctc
    17581 tttttgcgac aagtttaaaa cgtgcagatt ccttggctat cgctatggaa gcgcgtggct
    17641 atcagggtgg aaaaggcaga agtcaatacc gacaattgaa atggactcta aaggatacgc
    17701 tgaccattct tgttattctc gtacttggtt gttgtttatt tttcttaaaa tcttagtagc
    17761 tttcaggaat tgataaaagt tactgtaaca gtttttgata taaaggtagg gatatgaacc
    17821 gttttaaaaa atcaaaatat gtcattattg tttttgtcac tgttctgctt gtctcagctc
    17881 tcttagcgac gacttattca agtacaattg tgacaaaatt aggagatggg atctcattgg
    17941 ttgatagagt tgtgcaaaaa ccttttcagt ggtttgattc tgttaaatca gatttggcac
    18001 atttgacacg agcatataat gaaaatgaaa gtttgaagaa acagatttac caattagaag
    18061 ttaaatcaaa tgaggcggaa agtttaaaga cagaaaatga acaattgcgt caattgcttg
    18121 gtatgaagtc caagttgcaa gctacaaaga ctttggcagc agatgttatt atgcgttctc
    18181 cggtatcttg gaagcaggaa ttgaccttag atgtaggtaa atcaaaaggg gcttctgaga
    18241 acatgttagc tattgcaaat ggtggcttga ttgggagtgt ttcaaaagta gaggagaact
    18301 ctactatagt caaccttctg actaatacgg aaaatgctga taagatttct gttaaaattc
    18361 aacacggcac taccacaatt tatggaatta ttgttggcta cgacaaggaa aatgaggttc
    18421 ttaaaattag tcaattaaat agtaatagcg atattagtac aggcgataag gtgacaacgg
    18481 gtggattagg aaactttaac gttgcggata ttcctgttgg tgaagtagtt gccacaacgc
    18541 atagtacaga ctatttgaca cgagaagtaa ctgttaaatt gagtgcagat actcataatg
    18601 tggatgtgat agaattagtg gggaattcat aatgagacag ttgaagcgag ttggagtatt
    18661 tttattgctt cctttctttg ttctaattga tgcccatatt agccagcttc tgggctcatt
    18721 tttcccccat gtacatttgg ctagtcattt tctttttcta tttctcttat ttgagacgat
    18781 agaagtgtca gagtatctct acctagtcta ttgttttgtg ataggcttgg tttacgatgt
    18841 ttactttttc catctaatag ggattgcaac tctcttattt atcttattgg gagcctttct
    18901 tcataaattg aatagtgtta ttttattgaa tcgttggacg agaatcttgg ctatgattgt
    18961 tatgagtttt ctatttgata tgggaactta tctctttgca ttagtggtag gtttaactct
    19021 agatagtctg ccgattttta tcgtttatag cctagtacca tccatgattt taaatcttgt
    19081 gtggatgctt atttttcagt ttatttttga aagatattat ctataagaaa gaaacaaaaa
    19141 tgtaacaaag gcgtaatatt cattaggcct ttttttggta tactagtatt gtctttaaaa
    19201 gaaggagtat ctacgtaata tgaagaaaaa aatcttagcg tcacttttat taagtacagt
    19261 aatggtttct caagtagctg ttttaacaac tgcgcatgca gaaactactg atgacaaaat
    19321 tgctgctcaa gataataaaa ttagtaactt aacagcacaa caacaagaag cccaaaaaca
    19381 agttgaccaa attcaggagc aagtatcagc tattcaagct gagcaatcta acttgcaagc
    19441 tgaaaatgat agactacaag cagaatctaa aaaacttgag aatgagatta cagaactttc
    19501 taaaaacatt gtatctcgca atgattcttt ggaaaaacag gctcgtagtg ctcaaacaaa
    19561 tggagctgca actagctaca tcaatacgat tgtaaactca aaatcaatta cagaagctat
    19621 ttcacgtgta gcagcaatga atgaaatcgt atctgctaac aacaaaatgt tggaacaaca
    19681 aaaagcagat aaaaaagcaa tttctgaaaa acaagtagca aacaatgatg caattaatac
    19741 agtaattgca aatcaacaga aattgtctga tgatgcgcaa gctttaacaa cgaaacaagc
    19801 tgagttgaag gctgcagaat taaatcttgc tgctgaaaaa gcgacagcag aagatgaaaa
    19861 agcaagtttg ctagagaaaa aagcagaagc agaagtagca gcaaaagtag cagcagaggc
    19921 agaagcagct tacaaggcaa aacaagtaag tcaacaacaa acagttgttg cttctgggaa
    19981 tacgactttt gcagcacagg ttcaagctgt agcttcttcg gaatcagcaa catatactcc
    20041 tgtggcagtc aaacaacgcc caacatacag tacaaatgct tcaagctatc caattggaga
    20101 atgtacatgg ggagttaaaa cattagcacc ttgggctgga gactattggg gtaatggagc
    20161 gcagtgggct acaagtgcag ctgctgcagg attccgtaca ggttcaacac ctcaagttgg
    20221 tgcgattgca tgttggaatg atggtggcta tggacacgta gcggtagtca ctgctgtaga
    20281 atcaacaaca cgtatccaag tttcagaatc aaattatgct ggtaatcgca cgcttggaaa
    20341 tcatcgtggg tggttcaatc cgacaactac gtcttcaggt tttgtaacat atatttatgc
    20401 agattaattt acagagggac tcgaatagag ccctcttttc aggttttact gtgataatct
    20461 ctattaaaaa ttatatcaaa atagcttgaa aatattgaaa aagtatggta aaatgaaaat
    20521 tgtcgtgtga acgataatac tcattcttga tgaattgtga agcagttgcc cttgggtcgt
    20581 tttgcgagtt gaagtcaaga agaggaaaaa aaacaaaaag gagaaatact catggcagta
    20641 atttcaatga aacaacttct tgaggctggt gtacactttg gtcaccaaac tcgtcgctgg
    20701 aaccctaaga tggctaaata catctttaca gagcgtaacg gaatccacgt tatcgacttg
    20761 caacaaactg taaaatacgc tgaccaagca tacgacttca tgcgtgatgc agcagctaac
    20821 gatgcagttg tattgttcgt tggtactaag aaacaagcag ctgatgcagt tgctgaagaa
    20881 gcagtacgtt caggtcaata ctttattaac caccgttggt tgggtggaac tcttacaaac
    20941 tggggaacaa tccaaaaacg tatcgctcgt ttgaaagaaa tcaaacgtat ggaagaagat
    21001 ggaactttcg aagttcttcc taagaaagaa gttgcacttc ttaataaaca acgtgcacgt
    21061 cttgaaaaat tcttgggtgg tatcgaagat atgcctcgta tcccagatgt tatgtacgta
    21121 gttgacccac ataaagagca aatcgctgtt aaagaagcta agaaattggg aatccctgtt
    21181 gtagcgatgg ttgacacaaa cactgatcca gatgatatcg atgtaatcat cccagctaac
    21241 gatgatgcta tccgcgctgt taaattgatc acagctaaat tggctgacgc tatcatcgaa
    21301 ggacgtcaag gtgaagatgc agcagcagtt gaagcagaat ttgcagcttc agaagctcaa
    21361 gcagattcaa tcgaagaaat cgttgaagtt gtagaaggcg acaacgctta attcatataa
    21421 atagtaatta cctaggaggg cggggcttag cccggctctc ctatttttaa aaaatatagg
    21481 agaatcaaaa tggcagaaat tacagctaaa cttgtaaaag agttgcgtga aaaatctggt
    21541 gccggagtta tggacgctaa aaaagcgctt gtagaaacag acggtgacat cgaaaaagcg
    21601 attgaattgc ttcgtgaaaa aggtatggct aaggcagcta agaaggctga ccgtgttgct
    21661 gcagaaggat tgactggtgt ttatgttaac ggtaatgttg cagcagttat tgaagtaaat
    21721 gctgaaactg acttcgttgc gaaaaacgct caattcgttg aattggtaaa tactacagct
    21781 aaagttattg ctgaaggaaa acctgctaac aatgaagaag ctcttgcttt gacaatgcct
    21841 tcaggtgaaa ctcttgaagc tgcatacgta tctgcaacag caactatcgg agaaaaaatc
    21901 tcattccgtc gctttgcttt gattgaaaaa acagatgcac aacactttgg agcataccaa
    21961 cataacggtg ggcgtatcgg tgttatctct gtaatcgaag gtggagacga agcacttgct
    22021 aaacaaattt caatgcacat cgctgctatg aaaccaacag ttctttcata caaagaattg
    22081 gatgagcaat ttgttaaaga tgagttggca caattgaacc acgtaatcga ccaagacaac
    22141 gaaagccgtg caatggttaa caaaccagct ctaccacact tgaagtatgg atcaaaatct
    22201 caattgactg atgaagtgat tgctcaagct gaagctgaca tcaaagctga gttggctgca
    22261 gaaggcaaac cagaaaaaat ctgggacaaa atcatcccag gtaaaatgga tcgcttcatg
    22321 cttgacaaca ctaaagttga ccaagcttac acacttcttg cacaagttta catcatggat
    22381 gatagcaaga cagttgaagc atatcttgaa tcagttaacg cttcagtagt tgagttcgct
    22441 cgctttgaag ttggtgaagg tatcgaaaaa gctgcaaacg actttgaagc tgaagttgca
    22501 gctacaatgg cagcagcctt gaataactaa ttatagaaag gaagtaaatt tgcttccttt
    22561 tttctatgca gtcgctcctt atagggagaa cttcctattt tcaaggtaaa aataaaaagc
    22621 cctataataa gggccaattg tatttaggag actaaacttc aaattcatag agtgctgtag
    22681 agagataacg ttcaccatta tctggtgcta gagcaaggac ttttttacct gtacctaatt
    22741 ttttggcaat ctcgatggct ccgtagatag ctgcagctga agaaatccct acaaggaaac
    22801 cttcttttcc accaatttca cggccgagtg ctagagcatc gtctgacgtt acacgaacga
    22861 taccatcata agcctttgta tccagtgtat caggaataaa tccagctgag ataccttgaa
    22921 ttttgtgagg accaggtttt tcaccagata gaatagcaga ttcatcagct tccactgcat
    22981 aaacttgaat gtttgaattt gctgttttga gtgcatgaga aacaccagaa atcgttccac
    23041 cggtacctac tccagcaaca aaggcatcta atccatcttt accgaaagca gctagtatct
    23101 cagctcctgt tgttctttcg tgtacttctg gattagctgg attgtcaaat tgaagaggaa
    23161 ggaaaccatc acgttcagca gcgatttctt gagccttagc aatagcacct ttcattccct
    23221 cgctaccagg agttaggacg agttcagcac cataagcttg gataatttta cgtcgttcta
    23281 cactcatagt ttcaggcata acgatgacga ctttataccc tttagcagca cctacccatg
    23341 aaagtccaat accagtgttt ccacttgttg cttcaacaat agtagaacca ggtttcagaa
    23401 taccatcttg ttcagctttt tcaatcatgc taagggcaat acggtctttt acagatgaac
    23461 caggattaaa tgcttcaagc tttatataga cgtctgcagc accttctggc acgatgttgt
    23521 taagtttaac aatcggtgtt tgaccgatta attcagtaat gttgttataa atagacatat
    23581 gaatacctct ttgtataaga tatctttagt ataacgctat ctataccatt tgtaaaatat
    23641 agaaatccta tcgaagcgat aggatttcta tatatcatag atcaaagtca ttaattttca
    23701 agtgattgcg ttcagctaat tctagtaaat aggtgtgaag gaaaacgata tcaggtttcg
    23761 tttgaattgc ttcattttct agtatatttt ggtacgcatc tttttatttt gctcgctaaa
    23821 gtagttgtga gaatgtcaac gaaacgctgt attattcatt gtttacacta agtttacagg
    23881 aacttcaacc tctcgtaaac cttggtcagt taaagtgact tttccattaa aaaactccac
    23941 aagtgcagct ttaatagttt ctttttcttc cttatcaaca taaatcatcg tatcgacttg
    24001 atctgtaaag tttgtatcca gctccatgag atcatgttct ttaaggaagt tactgtactc
    24061 ttggtactga gcataagaca tttgaatagc aatgccagcc tgttctttta tttcaataat
    24121 accaatttct ttgacagcta aggcgacact gccggcgtaa gcacgaatta gtcctccagc
    24181 gcctagttta ataccaccaa agtagcgtgt cacgaccaca cagacattgg tgagattgtg
    24241 attttctagt accccaagca tgggaacacc agcagtacca ctaggctcac catcatcact
    24301 tgtacgttta atttcactac gttctccaat aataaaggca gagcagttat gtgtcgcttt
    24361 gtagtgttct tttttgatgg cagtaatgaa gtcacgagcc tcttcttcgc tataaacacg
    24421 cttggcatgg cagataaagc gagatttttt gatttcttct tggacttggc cgtcctcttt
    24481 aattgtccta aattccatga tttaattgta ctaaaaaatc atctaaagtg ctagttttta
    24541 cgaataaaga agtatgaaag taaatttaga ttatctcggt tgtttattta ctgagaatga
    24601 attaacagaa gaagaacgtc agttggcgga gaaacttcca gcaatgagaa aggagaaggg
    24661 gaaacttttc tgtcaacgtt gtaatagtac tattctagaa gaatggtatt tgcccatcgg
    24721 tgcttactat tgtcgagagt gcttgctgat gaagcgagtc agaagtgatc aaactttata
    24781 ctattttccg caggaggatt ttccgaagca agatgttctc aaatggcgcg gccaattaac
    24841 tccttttcaa gagaaggtgt cagagggact gcttcaagca gtagacaagc aaaagccaac
    24901 cttagttcat gcggtaacag gagctggaaa gacagaaatg atttatcaag tagtggctaa
    24961 agtgatcaat gcgggtggtg cagtgtgttt ggctagtcct cgcatagatg tttgtttgga
    25021 gctgtacaag cgcctgcaac aggatttttc ttgcgggata gctttgctac atggagaatc
    25081 ggaaccttat tttcgaacac cactagttgt tgcaacaacc catcagttat tgaagtttta
    25141 tcaagctttt gatttgctga tagtggatga agtagatgct tttccttatg ttgataatcc
    25201 cacgctttac catgctgtca agaatagtgt aaaggagaat ggattgagaa tctttttaac
    25261 agcgacttcg accaatgagt tagataaaaa ggtccgttta ggagaactaa aaagactgaa
    25321 tttaccgaga cggtttcatg gaaatccgtt gattattcca aaaccaattt ggttatcgga
    25381 ttttaatcgc tacttagaca agaatcgttt gtcaccaaag ttaaagtcct atattgagaa
    25441 gcagagaaag acagcttatc cgttactcat ttttgcttca gaaattaaga aaggggagca
    25501 gttagaagaa atcttacagg agcaatttcc aaatgagaaa attggctttg tatcttctgt
    25561 aacagaggat cgattagagc aagtacaagc ttttcgagat ggagaactga caatacttat
    25621 cagtacgaca atcttggagc gtggagttac cttcccttgt gtggatgttt tcgtagtaga
    25681 ggccaatcat cgtttgttta ccaagtctag tttgattcag attggtggac gagttggacg
    25741 aagcatggat agaccgacag gagatttgct tttcttccat gatgggttaa atgcttcaat
    25801 caagaaggcg attaaggaaa ttcagatgat gaataaggag gctggtctat gaagtgcttg
    25861 ttatgtgggc agactatgaa gactgtttta acttttagta gtctcttact tctgaggaat
    25921 gatgactctt gtctttgttc agactgtgat tctacttttg aaagaattgg ggaagagaac
    25981 tgtccaaatt gtatgaaaac agagttgtca acaaagtgtc aagattgtca actttggtgt
    26041 aaagaaggag ttgaagtcag tcatagagcg atttttactt acaatcaagc tatgaaggat
    26101 tttttcagtc ggtataagtt tgatggagac ttcctgttaa gaaaagtttt cgcttcattt
    26161 ttaagtgagg agttgaaaaa gtacaaagag tatcaatttg ttgtaattcc cctaagtcct
    26221 gatagatatg ctaatagagg atttaatcag gttgagggct tggtagaggc agcaggcttt
    26281 gagtatctgg atttattaga gaaaagagaa gagagagcca gttcttctaa aaatcgttca
    26341 gagcgcttgg ggacagaact tcctttcttt attaaaagtg gagtcactat tcctaaaaaa
    26401 atcctactta tagatgatat ctatactaca ggagcaacta taaatcgtgt taagaaactg
    26461 ttggaagaag ctggtgctaa ggatgtaaaa acattttccc ttgtaagatg aggaaaaaat
    26521 ttgcaaaaat agaatgaaag cgttataata aaatcataaa aacaaaaatg tttagaaaga
    26581 aggtactcat atgattaaat atagtatccg tggtgaaaac ctagaagtaa cagaagcaat
    26641 tcgtgattat gtagtttcta aactcgaaaa gatcgaaaag tacttccaac cagaacaaga
    26701 gttggatgcc cgaattaact taaaagttta tcgtgaaaaa acggctaaag tggaagtaac
    26761 gattccgctt ggatctatta ctctccgcgc agaagatgta tctcaagata tgtatggttc
    26821 aattgacctt gtaactgata aaattgaacg tcagattcgt aaaaataaaa caaaaatcga
    26881 gcgtaaaaat aaaaataagg tagcaactgg tcaattattt acagatgctt tggtggaaga
    26941 ttcaaatatt gtccagtcta aagttgttcg ttcaaaacaa attgatttaa aaccaatgga
    27001 tttggaagaa gcaattctac aaatggattt attggggcat gatttcttta tctatgtgga
    27061 tgttgaagat cagacaacca atgtgattta tcgtcgtgag gatggcgaaa ttggtttgtt
    27121 agaggttaaa gaatcttaat ttcaatatgt gtaaaggtag gtttactgaa ttgtaaacta
    27181 cctttttctt ataattgata gaattactga ttacactttt aaaaagtcgc tttttgaggg
    27241 gtattatggt ataatgggtg ccaagaggtt ttgaatgaaa aaattttatg taagtccaat
    27301 ttttcctatt ctagtaggat tgattgcgtt tggagtctta tccactttca ttatttttgt
    27361 taataataat ctgttgacgg ttttaatttt gtttcttttt gtaggaggct atgttttttt
    27421 atttaagaaa ctgagagtgc attatacaag gagtgatgta gaacagatac agtatgtaaa
    27481 ccaccaagcg gaagaaagtt tgacagctct attggaacag atgcctgtag gtgttatgaa
    27541 attgaattta tcttctggag aggttgagtg gtttaatccc tatgctgaat tgattttgac
    27601 caaggaagat ggtgattttg atttagaagc tgttcaaacg attatcaagg cttcagtagg
    27661 aaatccgtct acttatgcca agcttggtga gaagcgttat gctgttcata tggatgcttc
    27721 ttccggtgtt ttgtattttg tagatgtatc cagggaacaa gccataacag atgaattggt
    27781 aacaagtaga ccagtgattg ggattgtctc tgtggataat tatgatgatt tggaggatgc
    27841 aacttctgag tcagatatta gtcaaatcaa tagttttgta gctaatttta tatcagagtt
    27901 ttcagaaaaa tacatgatgt tttctcgtcg ggtaagtatg gatcgatttt atctatttac
    27961 tgactacacg gtgcttgagg gcttgatgaa tgataaattt tctgttattg atgctttcag
    28021 agaagagtcg aaacagagac agttgccctt gaccttaagt atgggatttt cttatggcga
    28081 tggaaatcat gatgagatag ggaaagttgc tttgctcaat ttgaacttgg ctgaagtacg
    28141 tggtggcgac caggtggttg ttaaggaaaa cgacgaaacg aaaaatccag tttattttgg
    28201 tggtgggtct gctgcttcaa tcaagcgtac acggactcgt acgcgcgcta tgatgacagc
    28261 tatttcagat aagattcgga gtgtagatca ggtttttgta gtcggtcaca aaaatttaga
    28321 catggatgct ttgggctctg ctgtaggtat gcagttgttc gccagcaatg tgattgaaaa
    28381 tagctatgct ctttatgatg aagaacaaat gtctccagat attgaacgag ctgtttcatt
    28441 catagaaaaa gaaggagtta cgaagttgtt gtctgttaag gatgcaatgg ggatggtgac
    28501 caatcgttct ttgttgattc ttgtagacca ttcaaagaca gccttaacat tatcaaaaga
    28561 attttatgat ttatttaccc aaaccattgt tattgaccac catagaaggg atcaggattt
    28621 tccagataat gcagtcatta cttatatcga aagtggtgca agtagtgcca gtgagttggt
    28681 aacggaattg attcagttcc agaattctaa gaaaaatcgt ttgagtcgta tgcaagcaag
    28741 tgtcttgatg gctggtatga tgttggatac taaaaatttc acctcgcgag taactagtcg
    28801 gacatttgat gttgctagct atctcagaac gcgcggaagt gatagtattg ctatccagga
    28861 aatcgctgcg acagattttg aagaatatcg tgaggtcaat gaacttattt tacaggggtg
    28921 taaattaggt tcagatgtac taatagcaga ggctaaggac acgaaatgct atgatacagt
    28981 tgttattagt aaggcagcag atgccatgtt agccatgtca ggtattgaag cgagttttgt
    29041 tcttgcgaag aatacacaag gatttatctc tatctcagct cgaagtcgta gtaaactgaa
    29101 tgtacaacgg attatggaag agttaggcgg tggaggccac tttaatttgg cagcagctca
    29161 aattaaagat gtaaccttgt cagaagcagg tgaaaaactg acagaaattg tattaaatga
    29221 aataaaggaa aaggagaaag aagaatgaaa gtaatctttt tagcagatgt taaaggaaaa
    29281 ggtaaaaaag gcgaaattaa ggaagtacca acagggtatg cgcaaaactt tcttatcaaa
    29341 aagaatctag ccaaagaagc gactgctcaa gctgtaggtg aacttcgtgg taaacaaaaa
    29401 tcggaagaaa aagctcacgc tgagatgatt gcagaaggaa aagcaattaa agcacaactt
    29461 gaagcagaag aaactgttgt agaatttgtt gaaaaagttg gtccagatgg tcgtaccttt
    29521 ggttctatta ccaataagaa gattgcagaa gaattgcaaa agcaatttgg aattaagatt
    29581 gataaacgtc atattcaagt acaagctccg attcgagcgg ttggtttgat tgatgtgcca
    29641 gtgaaaatct atcaagatat cacaagtgta atcaatcttc gtgtgaaaga aggataagtt
    29701 tacaccttct tgacaagatt gtaaaaggaa gggaagtctg atggcagaag tagaagagtt
    29761 acgagtacaa cctcaagata tcttagctga gcaatccgtt ttaggggcta tctttattga
    29821 tgagagtaaa cttgtttttg tgcgagaata cattgagtct cgggactttt ttaagtatgc
    29881 ccatcgtttg attttccaag ccatggtcga tttatccgat catggtgatg ccatagatgc
    29941 aacaacggtt cgtactatcc ttgataatca aggtgattta cagaatattg gtggcttgtc
    30001 ttacttggtt gagattgtta attctgtgcc aacttctgct aatgcggagt attatgctaa
    30061 gattgttgca gaaaaagcaa tgctacgtcg tttaattgcc aagttgacag agtctgtcaa
    30121 ccaagcttac gaagcgtcac aaccagctga tgaaattatt gctcaggcag aaaaagggtt
    30181 gattgatgtc agtgaaaatg caaatcgaag cgggtttaag aacattcgag atgtgttgaa
    30241 tctcaacttt ggaaatctgg aagctcgctc gcaacaaacg accgatatta caggtattgc
    30301 gacaggttat cgtgatttgg atcatatgac aacaggactt catgaggagg agttgattat
    30361 cttagcagct cgtccagcag ttggtaagac agcatttgcc ttgaatatcg ctcagaacat
    30421 tgggactaag ttggacaaaa cggttgctat tttttcactc gaaatgggtg cggaaagctt
    30481 ggtagatcgt atgttagctg cagaaggctt agtggagtca cattctatcc gtacagggca
    30541 attgacagat gaggagtggc aaaaatatac tattgctcag ggtaatctag ctaacgccag
    30601 tatctatatc gatgatacgc caggtattcg gattacagag attcgttctc gttctcgtaa
    30661 attggctcaa gaaactggaa atcttggttt gattttgata gactatttgc aacttatcac
    30721 gggaactggt cgagaaaatc gtcaacaaga agtttctgaa atttctcgtc agttgaaaat
    30781 actagccaag gaattgaagg ttccagtaat cgctctgagt cagctttctc gtggtgtaga
    30841 acaacgtcag gacaagagac cggtcttgtc tgatattcgt gaatctgggt ctattgagca
    30901 ggacgctgat atcgtagctt ttctctatcg cgatgactac tatgaacgtg gtggtgaaga
    30961 agaggagggt atcccaaata ataaggtgga agttattatc gagaaaaacc gtagtggagc
    31021 tcgtggaaca gtggaattga ttttccaaaa agaatacaat aaattttcaa gtatctcaaa
    31081 gagggaggca taagatgtca gatgcattta cagatgtagc caagatgaaa aaaatcaaag
    31141 aagaaatcaa ggcacatgag ggacaagtcg tagaaatgac tttggagaat ggtcgtaagc
    31201 gccaaaaaaa tagattgggt aagctaattg aagtttatcc atccctattt attgtggagt
    31261 ttggggatgt ggaaggagat aaacaagtta atgtttacgt tgaatccttt acttactcag
    31321 atattcttac agaaaagaat ttgattcatt atcttgacta aagtgagaaa ttttctcact
    31381 ttttcttttt tctccgaata atttaggtga aggcaatcat cgctttatat tatttttcaa
    31441 ggaggaagaa tgaaaatttt accgtttata gtaagaggaa caagttatta cttgaagatg
    31501 tcagttaaaa agcttgttcc ttttttagta gtaggattga tgctagcagc tggtgatagt
    31561 gtctatgcct attccagagg aaatggatcg attgcgcgtg gggatgatta tcctgcttat
    31621 tataaaaatg ggagccagga gattgatcag tggcgcatgt attctcgtca gtgtacttct
    31681 tttgtagcct ttcgtttgag taatgtcaat ggttttgaaa ttccggcagc ttatggaaat
    31741 gcgaatgaat ggggacatcg tgctcgtcgg gaaggttatc gtgtagataa tacaccgacg
    31801 attggttcca ttacttggtc tactgcagga acttatggtc atgttgcctg ggtgtcaaat
    31861 gtaatgggag atcagattga gattgaggaa tataactatg gttatacaga atcctataat
    31921 aaacgaatta taaaagcaaa cacgatgaca ggatttattc attttaaaga tttggatggt
    31981 ggcagtgttg ggaatagtca atcctcaact tcaacaggcg gaactcatta ttttaagacc
    32041 aagtctgcta ttaaaactga acccctagtt agtgcaactg tgattgatta ctattatcct
    32101 ggagagaagg ttcattatga tcaaattctc gaaaaagacg gctataagtg gttgagttat
    32161 actgcctata atggaagcta tcgttatgtt caattggagg ctgtgaataa aaatcctcta
    32221 ggtaattctg ttctttcttc aacaggtgga actcattatt ttaagaccaa gtctgctatc
    32281 aaaactgaac ccctagttag cggaactgtg attgattact attatcctgg ggagaaggtt
    32341 cattatgatc agatacttga aaaagacggc tacaagtggt tgagttatac tgcctataat
    32401 ggaagtcgtc gctatataca gctagaggga gtgacttctt cacaaaatta tcagaatcaa
    32461 tcaggaaata tctctagcta tggatccaat aatagttcaa ctgtcggttg gaagaaaata
    32521 aatggtagtt ggtatcattt caaatcaaat ggttctaaat caacaggatg gctgaaagac
    32581 ggttctagct ggtattattt gaaattatct ggtgaaatgc agacaggatg gttaaaggag
    32641 aatggctcgt ggtattatct gggtagttca ggggcaatga aaacaggctg gtaccaggtc
    32701 tctggtaagt ggtattattc ttattcttca ggcgccttag ctgttaatac gacggtggat
    32761 ggctacagag taaacagtga tggagaacga gtatagaaaa ttggagtagg agaaatttcc
    32821 tgctcttttc tgtaagcagt ttccttgaca tttttcttat tttgcgctat catatataca
    32881 tataatatat gggagtctgt gtacagtctg agaggaagtg ttaaacttcg accgcacctg
    32941 atctgggtaa tgccagcgga gggaacgata cttagtctaa ttttgcacct tttccatgta
    33001 tggtaaaggt ttttcttttt tttaaaagga aaacgagaag aggaggttct tatgaaagca
    33061 agcattgcct tgcaagtttt acccctagca caggggattg atcggatagc tgttattgat
    33121 caggtcattg cttatctgca aactcaagaa gtgacgatgg tagtgacacc atttgaaacg
    33181 gtcttggaag gggagtttga tgagcttatg cgcattctaa aagaagcgct ggaagtggca
    33241 gggcaggagg cagacaatgt ctttgccaat gtcaaaataa atgtaggaga gattttaagt
    33301 attgatgaga aacttgaaaa gtatactgag acgacacatt agtctattgg gctttctcgg
    33361 agtattgtca atctggcagt tagcaggttt tcttaaactt ctccccaagt ttatcctgcc
    33421 gacacctctt gaaattctcc agccctttgt tcgtgacaga gaatttctct ggcaccatag
    33481 ctgggcgacc ttgagagtgg ctttactggg gctgattttg ggagttttga ttgcctgtct
    33541 tatggctgtg ctcatggata gtttgacttg gctcaatgac ctgatttacc ctatgatggt
    33601 ggtcattcag accattccga ccattgccat agctcctatc ctggtcttgt ggctgggtta
    33661 tgggattttg cccaagattg tcttgattat cttaacaaca acctttccca tcatcgttag
    33721 tattttggac ggttttaggc attgtgacaa ggatatgctg actttgttta gtctgatgcg
    33781 agcaaaccct tggcaaatcc tgtggcattt taaaatcccg gttagcctgc cttactttta
    33841 tgcaggtctg agggtcagtg tctcctacgc ctttatcaca actgtggtat ctgagtggtt
    33901 gggaggtttt gaaggtcttg gtgtttatat gattcagtct aaaaaattgt ttcagtatga
    33961 taccatgttt gccattatta ttctggtgtc gattatcagt cttttgggta tgaagctggt
    34021 cgatatcagt gaaaaatatg tgattaaatg gaaacgttcg tagaattaga atgtttctga
    34081 aaaagaaaag aggaaatcaa aatgaagaaa acatggaaag tgtttttaac gcttgtaaca
    34141 gctcttgtag ctgttgtgct tgtggcctgt ggtcaaggaa ctgcttctaa agacaacaaa
    34201 gaggcagaac ttaagaaggt tgactttatc ctagactgga caccaaatac caaccacaca
    34261 gggctttatg ttgccaagga aaaaggttat ttcaaagaag ctggagtgga tgttgatttg
    34321 aaattgccac cagaagaaag ttcttctgac ttggttatca acggaaaggc accatttgca
    34381 gtgtatttcc aagactacat ggctaagaaa ttggaaaaag gagcaggaat cactgccgtt
    34441 gcagctattg ttgaacacaa tacatcagga atcatctctc gtaaatctga taatgtaagc
    34501 agtccaaaag acttggttgg taagaaatat gggacatgga atgacccaac tgaacttgct
    34561 atgttgaaaa ccttggtaga atctcaaggt ggagactttg agaaggttga aaaagtacca
    34621 aataacgact caaactcaat cacaccgatt gccaatggcg tctttgatac tgcttggatt
    34681 tactacggtt gggatggtat ccttgctaaa tctcaaggtg tagatgctaa cttcatgtac
    34741 ttgaaagact atgtcaagga gtttgactac tattcaccag ttatcatcgc aaacaacgac
    34801 tatctgaaag ataacaaaga agaagctcgc aaagtcatcc aagccatcaa aaaaggctac
    34861 caatatgcca tggaacatcc agaagaagct gcagatattc tcatcaagaa tgcacctgaa
    34921 ctcaaggaaa aacgtgactt tgtcatcgaa tctcaaaaat acttgtcaaa agaatacgca
    34981 agcgacaagg aaaaatgggg tcaatttgac gcagctcgct ggaatgcttt ctacaaatgg
    35041 gataaagaaa atggtatcct taaagaagac ttgacagaca aaggcttcac caacgaattt
    35101 gtgaaataat gacagaaatt agactagagc acgtcagtta tgcctatggt caggagagga
    35161 ttttagagga tatcaaccta caggtgactt caggcgaagt ggtttccatc ctaggcccaa
    35221 gtggtgttgg aaagaccacc ctctttaatc taatcgctgg gattttagaa gttcagtcag
    35281 ggagaattgt ccttgatggt gaagaaaatc ccaaggggcg cgtgagttat atgttgcaaa
    35341 aggatctgct cttggagcac aagacggtgc ttggaaatat cattctgccc ctcttgattc
    35401 aaaaggtgga taaggcagaa gctatttccc gagcggataa aattcttgcg accttccagc
    35461 tgacagctgt aagagacaag tatcctcatg aacttagcgg tgggatgcgc cagcgtgtag
    35521 ccttactccg gacctacctt tttgggcaca agctctttct cttagatgag gcctttagcg
    35581 ccttggatga gatgacaaag atggaactcc acgcttggta tcttgagatt cacaaacagt
    35641 tgcagctaac aactctgatc atcacgcata gtattgagga ggccctcaat ctcagcgacc
    35701 gcatctatat cttgaaaaat cgccctgggc agattgtttc agaaattaaa ctagattggt
    35761 ctgaagatga ggacaaggaa gtccaaaaga ttgcctacaa acgtcaaatc ttggcagaat
    35821 taggcttaga taagtagaaa aatagggagt tggtgaagat tatcctttac cagcgccctt
    35881 tttcttttaa aaatgagaaa atttcggtat aatagtcaaa caaggtcaag gtttaaagag
    35941 agaggtgggt ttgttatgag atttaaaaat acatcggatc atattgaggc ctacatcaag
    36001 gcgattttag atcaatctgg tatcgtggag ttgcaacgga gtcagttggc agataccttt
    36061 caggttgttc ctagtcagat taactacgtg atcaagacac gctttacgga aagtagaggc
    36121 tacttggttg aaagtaagcg tggtggcgga ggctacattc gtataggacg gattgagttt
    36181 tctagtcatc atgaaatgct ccgggatctg ctttactcga ttggtgagcg agtcagtcaa
    36241 gaaatttatg aggatattct ccagcttttg gttgagcagg aattgatgac caagcaggag
    36301 atgaatttgc tagaatcagt agctttggat cgcgttttag gagaagaagc tccagttgtt
    36361 cgagcaaaca tgctacgtca ggtcatacaa gaggtagata gaaaagggaa gtaagatgaa
    36421 ctattcaaaa gcattgaatg aatgtatcga aagtgcctac atggttgctg gacattttgg
    36481 agctcgttat ctagagtcgt ggcacttgtt gattgccatg tctaatcaca gttatagtgt
    36541 agcaggggca actttaaatg attatccgta tgagatggac cgtttagaag aggtggcttt
    36601 ggaactgact gaaacggact atagccagga tgaaaccttt acggaattgc cgttctcccg
    36661 tcgtttgcag gttctttttg atgaagcaga gtatgtagcg tcagtggtcc atgctaaggt
    36721 actagggaca gagcacgtcc tctatgcgat tttgcatgat ggcaatgcct tggcgactcg
    36781 tatcttggag agggctggtt tttcttatga agacaagaaa gatcaggtca agattgctgc
    36841 ccttcgtcga aatttagaag aacgtgcagg ctggactcgt gaagatctca aggctttacg
    36901 ccaacgccat cgtacagtag ctgacaagca aaattctatg gccaatatga tgggcatgcc
    36961 gcagactcct agtggtggtc tcgaggacta tacgcatgat ttgacagagc aagcgcgttc
    37021 tggcaagtta gaaccagtca tcggtcggga caaggaaatc tcacgtatga ttcaaatctt
    37081 gagccgtaag actaagaaca accctgtctt ggttggggat gctggtgtcg ggaaaacagc
    37141 tctggccctt ggtcttgccc agcgtattgc tagtggtgac gtgcctgcgg aaatggctaa
    37201 gatgcgcgtg ttagaacttg atttgatgaa tgtcgttgca gggacacgct tccgtggtga
    37261 ctttgaagaa cgcatgaata atatcatcaa ggatattgaa gaagatggcc aagtcatcct
    37321 ctttatcgat gaactccaca ccatcatggg ttctggtagc ggaattgatt cgactctgga
    37381 tgcggccaat atcttgaaac cagccttggc gcgtggaact ttgagaacgg ttggtgccac
    37441 cactcaggaa gaatatcaaa aacatatcga aaaagatgcg gctctttctc gtcgtttcgc
    37501 taaagtgacg attgaagaac caagtgtggc agatagtatg actattttac aaggtttgaa
    37561 ggcgacttat gagaaacatc accgtgtaca aatcacagat gaagcggttg aaacagcggt
    37621 taagatggct catcgttatt taaccagtcg tcacttgcca gactctgcta tcgacctctt
    37681 ggacgaagca gcagcaacag tgcaaaataa ggcaaagtat gtaaaagcag acgattcaga
    37741 tttgagtcca gctgacaagg ccctgatgga tggcaagtgg aaacaggcag cccagctaat
    37801 cgcaaaagaa gaggaagtac ctgtctacaa agacttggtg acagagtctg atattttgac
    37861 caccttgagt cgcttgtcag gaatcccagt tcaaaaactg actcagactg acgctaagaa
    37921 atatctaaat cttgaagcag aactccataa acgggttatc ggtcaagatc aagctgtttc
    37981 aagtattagt cgtgcgattc gccgtaatca gtcaggaatt cgcagtcaca agcgtccgat
    38041 cggttccttt atgttcctag gacccacagg tgtcggtaag accgagttgg ccaaggcttt
    38101 ggcagaagtt ctctttgatg acgaatcagc ccttatccgc tttgatatga gtgagtatat
    38161 ggagaaattc gcagccagcc gtctaaatgg agctcctcca ggctatgtgg gttacgaaga
    38221 aggtggggag ttgacagaga aggttcgcaa taaaccctat tccgttctcc tctttgatga
    38281 ggtagagaag gctcacccag atatctttaa tgttctcttg caggttctgg atgacggtgt
    38341 cttgacagat agcaagggac gcaaggtcga tttttcaaat accattatca ttatgacatc
    38401 gaacctaggt gcgactgccc ttcgtgatga taagactgtt ggttttgggg ctaaggatat
    38461 tcgttttgac caggaaaata tggaaaaacg catgtttgaa gaactgaaaa aagcttatag
    38521 accggaattc atcaaccgta ttgatgagaa ggtggtcttc catagcctat ctagtgacca
    38581 tatgcaggaa gtggtgaaga ttatggtcaa gcctttagtg gcaagtttgg ctgaaaaagg
    38641 cattgacttg aaattacaag cttcagctct gaaattgtta gcaaatcaag gatatgaccc
    38701 agagatggga gctcgcccac ttcgcagaac cctgcaaaca gaagtggagg acaagttggc
    38761 agaacttctt ctcaagggag atttagtggc aggcagcaca cttaagattg gtgtcaaagc
    38821 aggccagtta aaatttgata ttgcataaaa gaataaaagt accagcatct gaccataagt
    38881 cacagtggag tgaaactcaa tgaaaatcaa agagcaaact aggcagctag ccgcaggttg
    38941 ctcaaaacac tggtttgagg ttgcagatag agctgacgtg gtttgaagag atttttgaag
    39001 agtataagct gcaagatgaa tgattttctt gtattgacgt tgttgttgac aaaaagtagc
    39061 ggataaatga aatccattcc attatcatag atgataggct ggtaggaaat tttcaaatag
    39121 catacagaaa atagatgtat ggagttctgg tagtagaaag ggagagagat gaacatttta
    39181 gttgcagatg acgaggaaat gattagagaa ggaattgcag catttctgac agaagagggt
    39241 tatcatgtca ttatggctaa ggatggacaa gaggtcttgg aaaaatttca agatctccct
    39301 atccatctca tggtactgga tttaatgatg cctaggaaga gtggttttga agtgttaaaa
    39361 gaaatcaatc aaaagcacga tattcctgtc atcgtcttga gtgctctggg agatgaaact
    39421 actcagtcac aggtatttga tctctatgct gatgatcatg tgacaaaacc tttttctttg
    39481 gtactgcttg tcaagcgtat taaggcgctt atcagacgtt actacgtcat agaggatatt
    39541 tggcgatatc aggatgtaac agtggatttt acctcttaca aagcacatta taaaaatgaa
    39601 gaaattgatc tcaaaccaaa ggaattactg gtactaaagt gtttgattca gcataaaaat
    39661 caagttttaa gtagagagca gatattggaa gaaatttcaa aagatgtagc tgatttacct
    39721 tgtgataggg tcgttgatgt ctatattcgt actcttcgca aaaaattagc tttagattgt
    39781 atcgtgactg tgaaaaatgt tgggtataag attagcttat gataaaaaat cctaaattat
    39841 taaccaagtc ttttttaaga agttttgcaa ttctaggtgg tgttggtcta gtcattcata
    39901 tagctattta tttgaccttt cctttttatt atattcaact ggagggggaa aagtttaatg
    39961 agagcgcaag agtgtttacg gagtatttaa agactaagac atctgatgaa attccaagct
    40021 tactccagtc ttattcaaag tccttgacca tatctgctca ccttaaaaga gatattgtag
    40081 ataagcgact ccctcttgtg catgacttgg atattaaaga tggaaagcta tcaaattata
    40141 tcgtgatgtt agatatgtct gttagtacag cagatggtaa acaggtaaca gtgcaatttg
    40201 ttcacggggt ggatgtctac aaagaagcaa agaatatttt gcttttgtat cttccatata
    40261 catttttggt tacaattgct ttttcctttg ttttttctta tttttatact aaacgcttgc
    40321 tcaatcctct tttttacatt tcagaagtga ctagtaaaat gcaagatttg gatgacaata
    40381 ttcgttttga tgaaagtagg aaagatgaag ttggtgaagt tggagaagtt ggaaaacaga
    40441 ttaatggtat gtatgagcac ttgttgaagg ttattcatga gttggaaagt cgtaatgagc
    40501 aaattgtaaa attgcaaaat caaaaggttt cctttgtccg tggagcatca catgagttga
    40561 aaaccccttt agccagtctt agaattatcc tagagaatat gcagcataat attggagatt
    40621 acaaagatca tccaaaatat attgcaaaga gtataaataa gattgaccag atgagccact
    40681 tattggaaga agtactggag tcttctaaat tccaagagtg gacagagtgt cgtgagacct
    40741 tgactgttaa gccagtttta gtagatattt tatcacgtta tcaagaatta gctcattcaa
    40801 taggtgttac aattgaaaat caattgacag atgctaccag ggtcgtcatg agtcttaggg
    40861 cattggataa ggttttgaca aacctgatta gtaatgcaat taaatattca gataaaaatg
    40921 ggcgcgtaat catatccgag caagatggct atctctctat caaaaataca tgtgcgcctc
    40981 taagtgacca agaactagaa catttatttg atatattcta tcattctcaa atcgtgacag
    41041 ataaggatga aagttctggt ttgggtcttt acattgtgaa taatatttta gaaagctatc
    41101 aaatggacta tagttttctc ccttatgaac acggtatgga atttaagatt agcttgtaga
    41161 cagattagtt ttttattaaa gttcatatag ggttaacata agtgtgttat tctttgtata
    41221 gataaaagaa aggatactaa tatggtatta gcgattattt tagtaacatt ccttattcga
    41281 ttgatttttt taaagcgttc gatagagaat gagaaacgaa tccttagcaa tggcgggaaa
    41341 gaatttggag ttgagaatac aaaacgatta acgttggctc atattgtttt ttatctctct
    41401 tgcctcgttg aagcaatggt gcacaagaca atgtttgatg gtatgagcat ggttggttta
    41461 gtcttgctta ttttttctat gctgatgttg atgttggtga ttcacttgtt gggagatatt
    41521 tggacagtga agcttatgct tgtcaataat cacaaatatg tagatcatat cttgtttagg
    41581 acagtaaaac atcctaatta ctttttaaat attttacctg agttgattgg cttgaccttg
    41641 ttgagtcatg cctatatgac ttttgtttta gtttttccag tttatgcagt tattttgtat
    41701 cgacgaatag ctgaagagga aaagctatta catgaagtta taatcccaaa tggaagcata
    41761 aagagataaa tacaaaattc gactcatata cagttcatat tgaagtgata tagtaaggtt
    41821 aaagaaaaaa tatagaagga aataaacatg tttgcatcaa aaagcgaaag aaaagtacat
    41881 tattcaattc gtaaatttag tattggagta gctagtgtag tagttgccag cttgttctta
    41941 ggaggagtag tccatgcaga aggggttagg agtaagaata acctcacggt tacatctagt
    42001 gggcaagata tatcgaagaa gtatgctgat gaagtcgagt cgcatctaca aagtatattg
    42061 aaggatgtca ataaaaattt gaaaaaagtt caacataccc aaaatgtcga cttcaacaaa
    42121 aagttgagca gaattaaaac gaagtatttg tatggattaa aagagaagtc ggaagctgag
    42181 ttgacgttaa aaacaaaaga aacaaaagaa gagttaaccg cagcttttga gcagtttaaa
    42241 aaagatacat tgaaatcagg aaaaaaggta gcagaagctg agaaaaaagc caaagctcaa
    42301 aaagaagaag atcgccgtaa ctacccaacc aatacttaca aaacaatcga actggaaatt
    42361 gcggaagcag aagtaggggt cgccaaggca gagcttgagc ttgagtttgc acaagctcaa
    42421 gttcaaatac ctcaagatac tgagaaaatt aatgctgcta aatctaaagt agaagctgct
    42481 aaaagtaatg ttaaaaaact agaaaaaatt aaatcagata ttgaaaaaac gtatttgtat
    42541 aaattagata actcaaccaa agaaacgcca aaacctagag tgcgaagaaa ttctccggaa
    42601 ataaaagcta agggcagagt caaaaattat gaagaggcta atattgaact ttctaaatat
    42661 atgactgatt tgtataaatt agataactca accaaagaaa cgccaaaatc tagagtgcga
    42721 agaaattctc cgcaagtagg cgattcgaga gaacttaagg aaacgataga caaagcgaaa
    42781 gaaactctgt ctacctatat ggtaactcgt ttaacgaagc tggatccatc tgttttttgg
    42841 tttgcagatc ttcttatgga tgctaagaag gttgtggaag aatacaagac aaaattagag
    42901 gatgcttcag ataaaaaatc ggtagaagac ttgcgaaagg aagcagaagg aaaaatagag
    42961 tctcttatcg tgactcacca aaatagagaa aaagaaaacc aaccagcacc ccaaccagga
    43021 ggacaagcag gtggttcaat ggttgtacca ccggtgacgc aaacacctcc atcaacttcc
    43081 caaagtccag gacaaaaggc gaccgaagct gaaaagaaaa agttacaaga cttgattcgt
    43141 caattccaag aagccttgaa caaactagac gatgaaacaa agactgttcc agatggggct
    43201 aaactcacag gagaagctgg aaaagcctat aatgagacta gaacttatgc gaaagaagtt
    43261 gttgacaaaa gcaagaagct tctatcacag acagcagtga caatggatga attggcaatg
    43321 caattaacca aattgaacga tgccatgtct aaattgaaag aagctaaagc gaaattggta
    43381 ccagaggtta aaccacagcc ggaaaaccca gagccaaaac cacaaccaga gggtgagaaa
    43441 ccaagcgtac cagatattaa tcaggagaaa gaaaaagcta aacttgctat agcaacatac
    43501 atgagcaaga ttttagatga tataaagaaa catcatctga agaaagaaaa acatcatcag
    43561 attgttgctc ttattaagga ccttgataaa cttaaaaagc aagcactttc tgaaattgat
    43621 aatgtaaata ccaaagtaga aattgagaat acagtccaca aggtatttgc agacatggat
    43681 acggttgtta ctaaattcca aaaaggctta attcagaaca caccgcaggt tccagaagca
    43741 ccaaagagcc cagaggtacc aaaggtttca gatacaccaa aggctccgga cacaccgcag
    43801 gttccagaag caccaaagag cccagaggta ccaaaggttc cagaagcacc aaaggctcca
    43861 gacacaccgc aagttccgga agcaccaaag agcccagagg taccaaaggt tccagataca
    43921 ccaaaggctc cggacacacc gcaggttcca gaagcaccaa aggctccaga cacaccgcaa
    43981 attccggaag caccagctcc agaaactccg gctccagctc cagaagctcc aaaaacaggc
    44041 tggaaacaag aaaacggtat gtggtacttc tacaatactg atggttcaat ggcaacaggc
    44101 tggctagaat acaatggctc atggtactat ctcaacgcta atggtgctat ggc
//
LOCUS       DAVKJU010000009        43127 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVKJU010000009 DAVKJU010000000
VERSION     DAVKJU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.009"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(85..282)
                     /locus_tag="VW191_000408"
     CDS             complement(85..282)
                     /locus_tag="VW191_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9623240.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSSLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(357..1133)
                     /locus_tag="VW191_000409"
     CDS             complement(357..1133)
                     /locus_tag="VW191_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEU9623241.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1255..2001
                     /locus_tag="VW191_000410"
     CDS             1255..2001
                     /locus_tag="VW191_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9623242.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2014..2994
                     /locus_tag="VW191_000411"
     CDS             2014..2994
                     /locus_tag="VW191_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEU9623243.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3189..3509
                     /locus_tag="VW191_000412"
     CDS             3189..3509
                     /locus_tag="VW191_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9623244.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3555..3863
                     /locus_tag="VW191_000413"
     CDS             3555..3863
                     /locus_tag="VW191_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9623245.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3880..5178
                     /locus_tag="VW191_000414"
     CDS             3880..5178
                     /locus_tag="VW191_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9623246.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            5329..7767
                     /locus_tag="VW191_000415"
     CDS             5329..7767
                     /locus_tag="VW191_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEU9623247.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERAHKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7786..8454
                     /locus_tag="VW191_000416"
     CDS             7786..8454
                     /locus_tag="VW191_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU9623248.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            8472..9560
                     /locus_tag="VW191_000417"
     CDS             8472..9560
                     /locus_tag="VW191_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEU9623249.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10015..12516
                     /locus_tag="VW191_000418"
     CDS             10015..12516
                     /locus_tag="VW191_000418"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU9623250.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWIGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGDVKEAAYTEDGLHVNSDFLDELNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KAYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            12577..13638
                     /locus_tag="VW191_000419"
     CDS             12577..13638
                     /locus_tag="VW191_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623251.1"
                     /translation="MRTQSHKEIVEEYSASRGSYEDCLNYVENTVKNIIKSQSINVHE
                     IIGRVKTVESLEGKVKRKNYSNLAEITDLCGIRIITYFSDDVDKIAELISQEFEVDVE
                     NTIDKRKSEDPTKFGYVSLHYVVGLKEENTSPTLYGRFKNIKLEIQIRTVMQHAWAEI
                     EHDLGYKSKEDIPDQYRRQFARLAGLIELADDDFVQLKNNINNYEQEIKKKLPTSKKE
                     LPIDSSTLMTYVKEDQNYIELLNTIKSSDMDIDFNIDSSFLSTMIQRAKKLDLRTIGD
                     IAELFEKYKDIFLKVLRIEEDIEPCMSISALTPLLHITNVLESVNNINISDEEFMKVS
                     DDEFSDVFGISYPIDVYEG"
     gene            13865..14560
                     /locus_tag="VW191_000420"
     CDS             13865..14560
                     /locus_tag="VW191_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623252.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDRSTGKVLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAF
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMTDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            14572..14988
                     /locus_tag="VW191_000421"
     CDS             14572..14988
                     /locus_tag="VW191_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623253.1"
                     /translation="MITIKKQEFVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLAI
                     YVALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYCSMGFEILSTYDCIGMTWINREK"
     gene            complement(15840..15932)
                     /locus_tag="VW191_000422"
     CDS             complement(15840..15932)
                     /locus_tag="VW191_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9623254.1"
                     /translation="MMELILKTIIGPIVVGVILRLVDKWLNKDR"
     gene            complement(16273..16365)
                     /locus_tag="VW191_000423"
     CDS             complement(16273..16365)
                     /locus_tag="VW191_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEU9623255.1"
                     /translation="MMELVLKTIIEPIVVGVVLRLVDKWLNKDK"
     gene            16525..17496
                     /gene="ruvB"
                     /locus_tag="VW191_000424"
     CDS             16525..17496
                     /gene="ruvB"
                     /locus_tag="VW191_000424"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU9623256.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNDLEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGDGVINDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRMATAKAYEHLGYEYNEK"
     gene            17477..18050
                     /locus_tag="VW191_000425"
                     /pseudo
     CDS             17477..18050
                     /locus_tag="VW191_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
     gene            18281..19039
                     /locus_tag="VW191_000426"
     CDS             18281..19039
                     /locus_tag="VW191_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU9623257.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            19048..19851
                     /locus_tag="VW191_000427"
     CDS             19048..19851
                     /locus_tag="VW191_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU9623258.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFVLTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            19873..21132
                     /gene="rseP"
                     /locus_tag="VW191_000428"
     CDS             19873..21132
                     /gene="rseP"
                     /locus_tag="VW191_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU9623259.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIESILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            21145..22998
                     /locus_tag="VW191_000429"
     CDS             21145..22998
                     /locus_tag="VW191_000429"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU9623260.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            23097..24476
                     /gene="bglA"
                     /locus_tag="VW191_000430"
     CDS             23097..24476
                     /gene="bglA"
                     /locus_tag="VW191_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEU9623261.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            24668..26476
                     /gene="glmS"
                     /locus_tag="VW191_000431"
     CDS             24668..26476
                     /gene="glmS"
                     /locus_tag="VW191_000431"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU9623262.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSVGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHSYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            26595..27644
                     /locus_tag="VW191_000432"
     CDS             26595..27644
                     /locus_tag="VW191_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU9623263.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(27744..31541)
                     /gene="spuA"
                     /locus_tag="VW191_000433"
     CDS             complement(27744..31541)
                     /gene="spuA"
                     /locus_tag="VW191_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEU9623264.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYRTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGPVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            31958..32371
                     /gene="rpsL"
                     /locus_tag="VW191_000434"
     CDS             31958..32371
                     /gene="rpsL"
                     /locus_tag="VW191_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU9623265.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            32391..32861
                     /gene="rpsG"
                     /locus_tag="VW191_000435"
     CDS             32391..32861
                     /gene="rpsG"
                     /locus_tag="VW191_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU9623266.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            33286..35367
                     /gene="fusA"
                     /locus_tag="VW191_000436"
     CDS             33286..35367
                     /gene="fusA"
                     /locus_tag="VW191_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU9623267.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            35471..39862
                     /locus_tag="VW191_000437"
     CDS             35471..39862
                     /locus_tag="VW191_000437"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEU9623268.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            39964..40227
                     /locus_tag="VW191_000438"
     CDS             39964..40227
                     /locus_tag="VW191_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEU9623269.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            40220..40498
                     /locus_tag="VW191_000439"
     CDS             40220..40498
                     /locus_tag="VW191_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEU9623270.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            40519..40671
                     /locus_tag="VW191_000440"
     CDS             40519..40671
                     /locus_tag="VW191_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623271.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            40693..41934
                     /locus_tag="VW191_000441"
     CDS             40693..41934
                     /locus_tag="VW191_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU9623272.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEAAPVKAWEEHAAILKSKANMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            41944..42174
                     /locus_tag="VW191_000442"
     CDS             41944..42174
                     /locus_tag="VW191_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9623273.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            42397..43119
                     /locus_tag="VW191_000443"
     CDS             42397..43119
                     /locus_tag="VW191_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9623274.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT        12818 a         7601 c         9767 g        12941 t
ORIGIN      
        1 aatttgactt tcctgataga gatgtttaca tcttatttca attcgctata ttagagtaaa
       61 attctctaca aaaagaagaa tagcctattt tactattctt ccgagtgatt tcaatttctt
      121 tggggaaata tggagatact ttttaaatac tttggcaaaa tatttatagt ctgaaaatcc
      181 aacttggtca gagatagcgc ttaaagagga gttgggagat tcagccatct tatctatagc
      241 ttgtttcagg cgatattgta agatatattc atttagagtc atggggaagt cttctttgat
      301 aaccttatat agatgcttgt tatctgcaaa tagctagagc atttcaagga aataaactag
      361 aaataacaat taatatggtg gttcagaaat accttttgat aatcaataag atcttcagga
      421 tgaagtgcgt tgattccatc catttcatat ttccgattta ataaacgata tttgttttca
      481 ccaaattgat gaaaagggag tagttgaact tggtcgatat ttaatgagtt aaatagagta
      541 gcgaattttt ctgcatcctc taaactattg ttaaaattag gaataactgg gattcttaaa
      601 acgatagttt tattttgaga aaaagcataa tgaatgtttt taataatcat ttgattaaaa
      661 accccagtca cttttttatg ttttatagaa ttataatgtt ttaggtctgt gtagataaaa
      721 tccacatatt gaattaaatc gataaatttt tcatgatcaa caaaggcagt agtttcaatg
      781 gcagtgtgta tgtgatgttc tttagctgat tttaagatgg ctttagcaaa ttcaaactga
      841 gcaaatattt cacctcctga taaagttaaa cctccaccgg attcttcgta aaattctttg
      901 tcttttaata cctctgtaat aatttcttct acagtctttt cttcaccgac taaggtgaat
      961 ttctctcgtt gagcatcttt cattttttca ggtttcattc tttgagattc aggattagaa
     1021 caccatggac agcgcagagg acatcctttt aaaaaaacag ttgtacgaat acccgggccg
     1081 tcatgaattg aaaagtgttg aatattaaaa ataattcctt tagatatttc cataagagtt
     1141 cctcctcttc ataagtctat cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag
     1201 gttaatttgt tgttttttgc tttttatttt ggtagaataa aacggaggtg taaaatgaaa
     1261 agattggaac aaattattaa attagtatca gaatatgaaa agatcgacgt taatacatta
     1321 tcggaaaaat taaatgtatc gaaagtaacg attagaaaag atttagataa attagagtca
     1381 aaaggtttat tacacagaga gcatggatat gctgtattaa atagtggaga tgacttaaat
     1441 gtacgtttgt caattaatta tgaaattaag agaaaaattg ttcaggaagc agtaaaattg
     1501 gtgtcagata atgaaacaat aatgatagaa tctggatcga cctgtgcttt acttgctgag
     1561 gaaatttgca agcaaaaaag aaatgttacg attgtaacaa attcattttt tatagcaaat
     1621 tttgtgagag cttatgattc atgtcgtatt attgttcttg gtggagagtt tcagaaggat
     1681 tcacaggtta ctgtgggacc tttattaaaa gaaatgatac agacttttca tgtgcatcaa
     1741 gcttttgttg ggacagatgg ttacgataaa gagatgggct ttacaggaaa agatttaatg
     1801 cgcagtgagg tagttcaata tatttcagca gcgtcggata aagtcattgt actaactgac
     1861 tcaagtaaat ttgataaaag gggtacagta agaagatttg ctttaagtca agtctatgaa
     1921 gtaataacag acgaaaaact ttctaaacaa aatatagcta cattagaaaa tgctgggata
     1981 atggttaagg tagtttcgta agaggttaag tgtatgaatc aagataggaa taaactgctc
     2041 tctaaaattg cttatctgta ttatattgaa aacttaaatc agtcacaaat agcagcaaaa
     2101 ttaggaattt atagaacctc tattagtaga atgttaacag aagcaaggaa tgcaggaatt
     2161 gttaaaattg aaatagagaa ttttgatacc aatatgttta agttggaaaa ttatgtaaaa
     2221 gaaaaatatg gtctggaaag tttagaaatt attccaaatg aatttgatga taatccaaca
     2281 attttatctg aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct aattgatgat
     2341 aatatgaaaa ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac
     2401 agtaaaagtg tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac
     2461 gctaaatacc atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt
     2521 acatttatga atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa
     2581 tcaagatatt ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga
     2641 attggtgatt ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac
     2701 tttaaagaat tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca
     2761 aaaggtaaag aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat
     2821 ttaaaaaata ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt
     2881 ctttctgtct tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta
     2941 aaagttttgg aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga
     3001 tagactgatt cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa
     3061 caaaaatagt ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata
     3121 gttacaaaag aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg
     3181 aggaatttat ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg
     3241 gcgatgcgaa acaacatatt tataaagcat tagattacat aaagaacggt acatgtgaac
     3301 gatgtgaaga agaaatccag ttagctgatg cagccttatt agaagctcat aatctacaaa
     3361 caaaattctt ggcacaggaa gcgtctggta caaagacaga aattacagct ctctttgttc
     3421 attcacaaga tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta
     3481 gtttgagaaa agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag
     3541 aggaggaaaa cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta
     3601 tgcttgtaaa taatatgaaa attgcagcgc aatctagtgg agttgaggca gaaatagagg
     3661 cgttttctca gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc
     3721 cacaagttgc ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga
     3781 tagctgttat tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg
     3841 ccctatcttt gattagtggg taagaaaagg agatttatta tgtcaaagat ggatgttcag
     3901 aaaatcattg caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat
     3961 gggatgttag caattttgcc attgacagta gttggtagtt tgttcttgat tatgggacaa
     4021 ttgccgttcg aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag
     4081 ccgtttatgc aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca
     4141 attgcctatt cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct
     4201 gtatctgcat tctttatttt gctaagatca tcttatatcc ctaaacaagg tgaggcgatt
     4261 ggggacgcta ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc tatcattata
     4321 ggtttggtag taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg
     4381 ccagaacaag ttccacaagc tattgcgaaa cagtttgaag caatgattcc agcatttgta
     4441 attttcttat cttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca
     4501 ttcatagaaa tgatttattc tgctattcaa gttccgttgc aaggtttaac tggatctttg
     4561 tatggtgcta ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatggg
     4621 caatcggtag taaatggagt agtgacagct ctgcttttat ctaatcttga tgctaataaa
     4681 gctatgttag cctctggtaa tctatcatta gaaaatggcg cacatattgt tactcaacaa
     4741 tttttagatt catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc
     4801 atgctttttg cagcaaaatc aaaacaatac caagccttag gaaaagttgc agcttttcca
     4861 gcaatattta acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt
     4921 atgtttgtac ctttcattct tgttcctgta cttgcagctg tgatagtata tggagctatt
     4981 gcaacaggtt tcatgcagcc attctcaggg gtaacattgc cttggagtac accagctatt
     5041 ctatcaggat ttttggtggg tggatggcaa ggtgttgtga ctcagttatt agtattggtg
     5101 atatctacag tggtttactt tccattcttt aagatacaag atcgtttagc ttaccaaaat
     5161 gaaatcaaac aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgctaa
     5221 aaattagaga gttaaaattt ttctagttaa aagcttgaaa atttctataa aaatcggtat
     5281 tatattttcg aaagaaataa aaatattttc gaaagaaagg tggttacgat ggtaaataca
     5341 gaagtagcaa gaacaacaat caagacagaa tattttggca gccttactga aaggatgaac
     5401 aaatatcgag aagatgtttt aaataaaaaa ccttatattg atgctgagag agcagttcta
     5461 gcaacacgcg cctatgaacg atacaaggaa caacctaatg tcctaaaacg tgcatatatg
     5521 ctgaaagaaa ttttggaaaa tatgtctatc tatattgaag aagaatctat gattgcggga
     5581 aatcaagctt cttccaataa agatgctcct atttttccgg aatatacgct agaatttgtt
     5641 ctcaatgagt tggatctttt tgaaaagcgt gatggagatg ttttctatat tacagaagaa
     5701 acaaaagaac aacttagaag tattgctccg ttttgggaaa ataataattt acgtgctaga
     5761 gctggtgcct tattacctga agaagtgtct gtttatatgg aaacaggatt cttcggtatg
     5821 gaaggtaaga tgaattctgg agatgctcac ttagcagtta actatcagaa acttttgcaa
     5881 tttggtttaa gaggttttga agagcgggct cataaagcaa aagtagctct agatttaaca
     5941 gatccagcaa gtattgataa atatcatttt tacgactcta tatttatcgt aatcgatgct
     6001 attaaagtat atgcaaagcg ctttgttgct cttgctaaaa gtttagccga aaatgcaaat
     6061 cctaaacgta agaaagaatt acttgagatt gcagatattt gctctagagt cccatatgaa
     6121 ccggcaacta cttttgcaga agctattcaa tcagtttggt ttattcaatg tattttacaa
     6181 attgaatcta atggccactc tctttcatat ggccgttttg atcaatatat gtatccatat
     6241 atgaaggctg atttagaaag tggtaaagaa acagaagata gcattgttga acgtctgaca
     6301 aatctttgga ttaagacaat tacaattaat aaggttcgca gtcaatcaca tacattttct
     6361 tcagcaggaa gtcctttata tcaaaatgtt acaattggtg gacagactcg agataagaag
     6421 gatgctgtta acccattatc ttatttggta ttaaaatcag ttgcacaaac ccatctaccg
     6481 caacctaatc taactgtacg ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt
     6541 attgaagtga tgaaacttgg ttttggtatg cctgcattta ataatgatga gattattatt
     6601 ccttctttta ttgcaaaagg agtattggaa gatgatgctt atgattacag tgccattgga
     6661 tgtgttgaaa cggcagttcc agggaaatgg ggctatcgtt gcacaggtat gagttatatg
     6721 aacttcccta aggttctact tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa
     6781 cggtttgcac caagctttgg tcattttaag gatatgaaga acttttctga attagaaaat
     6841 gcttgggata aaacactaag atatttgaca cgaatgagtg ttattgttga aaattctatt
     6901 gatttatcat tggaacgaga agttcctgat attctatgtt cagcattgac tgatgattgt
     6961 attggtcgtg gaaaacacct taaagaaggt ggagcagtat atgattatat atcaggattg
     7021 caagttggaa ttgcaaattt gtcggattcg ttagctgcaa ttaaaaaatt ggtgtttgag
     7081 gaagaacgta taagcccaag tcaactttgg catgcactgg aaacagatta tgccggagaa
     7141 gaaggtaagg tcattcaaga aatgttgatt catgatgcac ctaagtatgg taatgatgat
     7201 gattatgctg acaaattggt tactgctgct tatgacattt atgttgatga aattgctaaa
     7261 tatcctaata cacgttatgg aagagggcct attggaggaa ttcgttattc aggaacatct
     7321 tctatatcag ccaacgtagg gcagggacgt ggaacattag caactccaga tggacgcaac
     7381 gcgggtacac cgttagcaga gggttgttca ccatcacata atatggatca acacggccct
     7441 acatctgttt taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg tggggttctc
     7501 ttaaatcaga aagtaaatcc tcaaacgtta gccaaagaag aagataaatt aaaactaatt
     7561 gctttgttac gaacattctt taatcgttta catgggtacc atattcaata caatgttgtt
     7621 tccagagaga cgctgattga cgctcagaaa catcctgaaa aacacagaga cttaattgtt
     7681 cgtgttgcag gatactctgc attcttcaat gttctttcta aggcaaccca agatgacatt
     7741 ataggacgta ctgagcatac tttgtaaaat aaagaggttc tttttatgga atttatgctt
     7801 gacacattaa atttagatga gattaaaaag tggtctgaaa ttttgccgct agctggggta
     7861 acttcaaatc ccactattgc aaaaagagag ggttctatta atttttttga acgaatcaaa
     7921 gatgtaagag aattgattgg ctctacaccc tctattcatg ttcaggtgat ttctcaagat
     7981 tttgaaggca tcttaaagga tgctcatgaa attcgaagac aagcaggaga tgatatattt
     8041 atcaaagtac ctgttactcc agctggatta cgtgcaataa aggtgctaaa aaaagagggc
     8101 taccatatca ctgcaacagc tatttataca gttattcagg gattattagc tatcgaagca
     8161 ggagcggatt acctagctcc atattataat agaatggaaa atctgaacat tgattcaaat
     8221 tctgtcattc gtcaattagc tcttgctatt gatagacaga actctcctag taagatttta
     8281 gctgcatcct ttaaaaatgt agcacaagta aataatgctt tagctgcagg tgcgcatgct
     8341 gttacagcag gagcagatgt ttttgaatca gctttcgcca tgccatctat ccaaaaggcg
     8401 gttgatgatt tttctgacga ttggtttgtt actcaaaata gtcgttccat ttagatagag
     8461 aggaaatata tatgagaatt tttgctagtc cttctagata tattcagggg gaaaatgcct
     8521 tgtttgaaaa tgccaaatca attttggatt tgggaaatta ccctattcta ttatgcgatc
     8581 agttggttta tgatattgtt ggaaaacgat ttgaagatta cctacatagg tatggtttcc
     8641 atattgttct ggcgctattt aatggtgaag cttctgacaa tgaaatcaat cgagttgttg
     8701 ccttggctga gaaagaaaat tgtgatagta ttatcggtct tggtggggga aagacgattg
     8761 atagcgcaaa agctattgca gatttgattg aaaagcctgt tattattgct ccaacaattg
     8821 catcgaccga cgcacctgta tctgctttat ctgttattta tacagatgaa ggtgcatttg
     8881 atcattatct attttattct aaaaatccag atttagtttt ggttgataca aaagttattt
     8941 cacaagcccc taagcgttta ttagcgtctg gtattgcaga tggtttagca acttgggttg
     9001 aggcgcgtgc ggttatgcag gcaaatggaa aaactatgtt gggacaacag caaacattgg
     9061 ctggagttgc aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta
     9121 tggcagcttg tgaagctaaa gtggtgacac cagcattaga aaatattgtt gaagctaata
     9181 ctttattgag tggtctaggt tttgaaagtg gaggattagc tgcggcgcat gcaattcata
     9241 atggttttac tgcattgaca ggtgacattc atcatttaac acatggtgaa aaagtagctt
     9301 atggaacttt agtacaacta ttattggaaa atagacctaa agaagaactt gataagtata
     9361 ttgagtttta caaaaaaatt ggtatgccaa caactctaaa agaaatgcat ttggatcaag
     9421 ttggatatga tgatttaata aaagttggta aacaagcaac tatggagggt gagacaattc
     9481 atcagatgcc gtttaagatt tcgccttcag atgttgctca agctattatc gctgtagatg
     9541 cctatgtaaa ttcaaaataa acaataagga ctactgtttt ccaaatggta gtcttttatt
     9601 gatcctcgta ttgaattcta tagcagattg aaataagatg agaacaaatc gattgggaaa
     9661 gtaaaattaa tttctataaa tgttttagca attgtttcgt actatttcag attcagtcca
     9721 ctatacgttc ttcataaatc aaaaagcgac ataggtagtc ggctatttat tgtgaataca
     9781 ttaattagca ttccagtttt atcttcggtc taaaataagt attttgtgct atacgagata
     9841 agcgtcttga cttactcctt gatttactgc ataacaatgg gataaaaagt gggagataga
     9901 gcaattcata gtcatcaaaa ttaatgagat acagtataca gttttttctt taaacacatt
     9961 tcaaattccc tcaaaaatgg tataatagta acatcacaaa attggagaga gaccatgagt
    10021 ttttacaatc ataaagaaat tgaacctaag tggcagggct actgggcaga acatcataca
    10081 tttaagacag gaacagatgc atcaaaacct aagttttatg cgcttgatat gttcccttat
    10141 ccgtctggag ctggtctgca tgtaggacac ccagaaggtt atactgcaac cgatatcctc
    10201 agtcgttaca aacgtgcgca aggctacaat gtccttcacc caatgggttg ggatgctttt
    10261 ggtttacctg cagagcaata cgctatggat actggtaatg acccagcaga atttacatcg
    10321 gaaaacattg ccaacttcaa acgtcaaatc aatgcgcttg gattttctta tgactgggat
    10381 cgtgaagtca acacaacaga tccaaactac tacaagtgga ctcaatggat tttcaccaag
    10441 ctttacgaaa aaggcttggc ctatgaagct gaagtgccag taaactgggt tgaggaattg
    10501 ggaactgcca ttgccaatga agaagtgctt cctgacggaa cttctgagcg tggaggctat
    10561 ccagttgtcc gcaaaccaat gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc
    10621 ttgctcaatg acttagatga actagattgg tcagagtcta tcaaggatat gcaacgcaac
    10681 tggattggta aatcaactgg tgccaatgta actttcaaag taaaaggaac agacaaggaa
    10741 tttacagtct ttactactcg tccggacaca cttttcggtg cgactttcac tgtcttggct
    10801 cctgaacatg aattagtaga cgctatcaca agttcagagc aagcagaagc tgtagcagac
    10861 tataaacacc aagccagcct taagtctgac ttggctcgta cagaccttgc taaagaaaaa
    10921 actggtgttt ggataggtgc ctatgccatc aaccctgtca atggtaagga aattccaatc
    10981 tggattgcgg attacgttct tgctagctat ggtacaggtg ccgttatggc tgtgcctgcc
    11041 cacgaccaac gtgactggga atttgccaaa caatttgacc ttccaatcgt cgaagtactt
    11101 gaaggtggag atgtcaaaga agctgcctac acagaggatg gcctgcatgt caattcagac
    11161 ttcctagatg aactcaacaa agaagacgct attgccaaga ttgtggcttg gttggaagaa
    11221 aaaggctgtg gtcaggagaa ggttacctac cgtctccgcg actggctctt tagccgtcaa
    11281 cgttactggg gtgagccaat tccaatcatt cattgggaag atggaacttc aacagctgtt
    11341 cctgaaactg aattgccgct tgtcttgcct gtaaccaagg atatccgtcc ttcaggtact
    11401 ggtgaaagtc cactagctaa cttgacagat tggcttgaag tgactcgtga agatggtgtc
    11461 aaaggtcgtc gtgaaaccaa cactatgcca caatgggctg gttcaagttg gtactacctc
    11521 cgctatattg atccacacaa tactgagaaa ttggctgatg aggacctcct caaacaatgg
    11581 ttgccagtag atatctacgt gggtggtgcg gaacatgctg tacttcactt gctttacgct
    11641 cgtttctggc acaaattcct ctatgacctc ggtgttgttc cgactaagga accattccaa
    11701 aaactcttta accaagggat gattttggga acaagctacc gtgaccaccg tggtgctctt
    11761 gtggcaaccg acaaggttga aaaacgtgat ggttccttct tccatgtaga aacaggggaa
    11821 gagttggagc aagcgccagc caagatgtct aaatcgctca agaacgttgt taacccagac
    11881 gatgtggtgg aacaatacgg tgccgatacc cttcgtgttt atgaaatgtt tatgggacca
    11941 ctcgatgctt cgattgcttg gtcagaagaa ggtttggaag gaagccgtaa gttccttgac
    12001 cgagtttacc gtttgattac aagtaaagaa atccttgcgg aaaacaatgg tgctcttgac
    12061 aaggcttaca acgaaacagt caaagctgtt actgagcaaa ttgagtctct caaattcaac
    12121 acagctattg cccaacttat ggtctttgtc aatgctgcta acaaggaaga taagctttat
    12181 gttgactatg ccaaaggctt tattcaattg attgcaccat ttgcacctca cttggcagaa
    12241 gaactctggc aaacagtcgc agaaacaggt gagtcaatct cttatgtagc ttggccaact
    12301 tgggacgaaa gtaaactggt tgaagacgaa atcgaaatcg ttgtccaaat caaaggtaaa
    12361 gtccgtgcca aactcatggt cgctaaagac ctatcacgtg aagaattgca agaaattgct
    12421 ctagcggatg aaaaagtcaa agcagaaatt gacggtaagg aaatcgtgaa agtgattagt
    12481 gtaccaaata aactcgttaa tatcgttgtg aaataattgt aaattatagt taattaaaaa
    12541 gagagacagt ttttgttcac tttttgggag gtattaatga ggactcaaag tcataaggag
    12601 attgttgaag aatattctgc ttcacggggg agttatgaag attgcttaaa ttatgtagag
    12661 aatactgtaa agaatattat taaaagtcaa tctattaacg ttcatgaaat aattgggaga
    12721 gtaaaaactg tagagtctct tgaaggcaaa gtaaaaagga aaaattattc taatttagct
    12781 gagattaccg atttatgcgg tattcgtatc atcacgtact ttagtgatga tgttgataaa
    12841 atagctgagt taatcagtca agaattcgag gttgatgtag agaatactat tgataaacgt
    12901 aaaagtgagg atccaacaaa atttggttat gtttcacttc attatgtggt gggtttaaaa
    12961 gaagagaaca cttcacctac tttatatggt agatttaaga atataaaatt agagatacaa
    13021 attaggacag ttatgcaaca tgcctgggct gaaattgaac atgatttggg atataaaagt
    13081 aaggaagata tacctgatca atacaggaga caatttgcca gattggcggg actaattgaa
    13141 ttggctgatg atgattttgt tcagttgaaa aataatatta ataactatga gcaggagata
    13201 aagaagaaat tacctacttc gaaaaaagaa ttaccgatag atagcagcac gcttatgact
    13261 tatgtaaaag aagatcaaaa ttacattgaa ctattgaata cgattaaatc atcagatatg
    13321 gatatagatt ttaatattga tagttctttt ctatctacta tgattcagag ggcgaaaaaa
    13381 ttggatttga ggactatagg agatatagct gaattatttg aaaaatataa ggatatcttt
    13441 ctaaaggtat tgagaattga ggaggatatt gaaccttgta tgagtatatc ggcattgact
    13501 ccattattgc atatcactaa tgtgttagaa tcagttaata atattaatat ttctgatgaa
    13561 gaatttatga aagtttctga tgatgaattt tcagatgtat ttggtatatc gtacccaatt
    13621 gatgtttatg aaggttaaaa agtaattttt taaatatgaa aagaggggtt tcctatttga
    13681 ctaagttgaa taattcgtag tgttgctctt tctaaagtga aggtaactaa actcgtcaat
    13741 atcgttgtta gataagaaat aaatccttca gagtagaatc tggaggattt tttgaatctt
    13801 cttatgaaag tatgatatac tatgggcaac tataaagttt gaaaagtgaa acaaggagaa
    13861 taagatgcca gtaaatgaat atggtcagat gattggggag tcaatggaag gttatacacc
    13921 aggtgaactg ccttctattg atttcttaga aggacgttat gctcggatag aggctctctc
    13981 ggtagaaaaa catgcggagg atttgttagc tgtttatggc ccggatactc ctcgggagat
    14041 gtggacctac ctctttcagg aaccagtagc agacatggag gaactggtca gccttttaaa
    14101 tcagatgttg gctcgtaagg accgttttta ctatgcaatc atagacaggt caactggtaa
    14161 ggttttggga actttttctc tcatgcgcat tgaccagaat aaccgagtaa tagaagtggg
    14221 agctgtcact ttttctccag aactcagggg gacacggata gggacagaag cccagtatct
    14281 actggctcgc tatgtctttg aagagcttaa ctatcgtcgc tatgagtgga aatgcgatgc
    14341 ttttaatctg ccatcaagac gagctgcaga acgtttgggc tttatttatg aaggaacctt
    14401 ccgtcaggca gtggtttata aggggcgtac gagggatacg gattggttgt ctatgactga
    14461 taaggactgg cccaaagtca aagatcgttt ggaaacatgg ttgcgtcctg aaaattttga
    14521 taaaaatgga cagcagtaca agagcttgag agagctttaa gaggtgttga gatgattact
    14581 attaaaaagc aagaatttgt caagctagag gatgttttgc atctctatca ggctgtcggt
    14641 tggacaaatt atacccatca accagagatg ctggagcagg ccttatctca ttcattagcg
    14701 atttatgtgg cgcttgatgg cgatgctgtg gtgggcttga ttcgtttggt tggagatgga
    14761 ttctcatcag tattggttca ggatttaatc gttttaccaa tctatcaacg tcaagggatt
    14821 ggtagtgccc taatgaaaga ggctttagag gattacaaag atgcctatca agtccagctg
    14881 gtaacagaac agacagaaag aaccttgggc ttctattgtt ctatgggctt tgaaatctta
    14941 tccacctatg attgtatagg aatgacttgg ataaacagag aaaaataata aaacttggtt
    15001 gttcttaagc aaagtttaag gctggtctag tatcatatag tcattaaata aagacctcct
    15061 aactttattt aatgaaatcc taaaactttt tttcatcata atctcctaat gaagtcacct
    15121 aatcaggtgg cttttttgta gggtgaggtg atgctgatag aaattttttt aaaaatggta
    15181 taatttgaga aaagttaagc tagttttaag cttcgtcttg tatcatatag ttattaaata
    15241 aagacctcct aactttattt aataaaatcc taaacttttc tttttcataa taatctccct
    15301 taactccacc caatcaggtg gagttttttg gctctatttc aggcttttgg ggactattct
    15361 aaaaataatt tttcgatatt tttcggtatt tttcggattt tggtcgggga attggcgggg
    15421 actttttgag attttggcgg ggattttttt agcgaatatg actaagaaat aggtcggttg
    15481 tcgcttcagc aagttcgtcc tcaacttgat tgtaagttgc tgttaattga aggactggaa
    15541 ctgctgtaat aatgattttg cttgtccagt agtggcgtat taaatgagga gtgacatata
    15601 agattgtctc ttcgtttact aaatcaaaat ttctatgaaa ttgatagaat ccctaacttt
    15661 ctcttaaaat tgaaagtctt gttgtaaagc ggttaaagct tgagaagctg aggatgtaaa
    15721 gttttgacaa attttgtgaa ctgtgggata atggagagga attaaggatt agttctatat
    15781 catggactag aaaagacccc aagctaactt ccacattaag cttggggtct tttttgacac
    15841 tatctatctt tgtttagcca tttatcgact aagcgaagga tgacaccgac cacaatcggt
    15901 ccgatgatag ttttaaggat tagttccatc atgggctatc tcacctcctt tcgcaagcgg
    15961 tgtagcagtg ccgtagaata ttataccaca taagtgctaa actcgggagt cacccaacca
    16021 ggtggagttt tttgctctat ttcaggcttt tggggactat tttaaacatc attttccgat
    16081 aactttcact taatcttata aactttattg gacagattct aaagttggag aagctgaatg
    16141 gatgaaaata taaaactttg acaaaatgtg tgaactatgg gataataaaa aggaattgag
    16201 tactagttct atatcatagg ctagaaaaga ccccaagctc acgaccaaat aagcttgagg
    16261 tcttttttga cactatttgt ccttgtttag ccatttatcg actaatcgaa gaacgacacc
    16321 gaccacaatt ggttcgatga tagttttgag tactaattcc atcatgggct atctcacctc
    16381 ctttcgtagg cggtgtagaa gtgccgtata atattatacc acataagtgc taaactcggg
    16441 aagcaccaat ccagtgactt ttttgtttgt ggcttggttt tttgatataa tagagccatg
    16501 agtagaattt tagataatga gataatgggg gacgaggagt tagtagaacg cacgctccgt
    16561 cctcagtact tacgtgaata tattgggcag gataaggtta aggaccagct tcaaatcttt
    16621 atagaagctg ccaaaatgcg agatgaagcg ctggatcatg tcctcttatt tgggcctcca
    16681 ggtttgggga aaacgaccat ggcttttgtt attgccaacg aactaggtgt caatctcaag
    16741 cagacttcgg gtccagtcat tgaaaaagcc ggagatctgg tagcaatttt gaatgactta
    16801 gagcctgggg atgtcctctt tattgatgag attcatcgct tgcctatgtc ggtggaagag
    16861 gtgctttata gtgccatgga ggacttttac atagatatta tgattggggc tggtgaaggc
    16921 agtcgcagtg ttcatttgga gttaccacct tttaccttga ttggtgcgac gactcgggct
    16981 ggtatgctct caaatccact acgggcacgt tttgggatta caggtcatat ggagtattat
    17041 gctcatgctg acttgacgga aattgtcgag cggacggcag atatttttga gatggaaatc
    17101 actcatgagg cagcgtctga gttggcccta cgtagccgtg gaacccctcg tattgccaat
    17161 cgtctcctca agcgcgtgcg cgattttgcc cagattatgg gggatggggt tatcaatgat
    17221 gttattacgg ataaggcttt gaccatgctg gatgttgacc atgaaggtct ggactatgtg
    17281 gaccaaaaaa tccttcggac catgattgag atgtacagtg gagggcctgt tggtttagga
    17341 actctttctg tgaatattgc cgaggagcgc gagacggtgg aggacatgta tgaaccttac
    17401 ttaatccaaa aaggttttat catgcggaca cggtctggac ggatggcgac tgctaaggca
    17461 tatgagcatt tggggtatga gtacaatgaa aaataagcaa gaaattctag aggcttttag
    17521 aaaaaatcca gatatgatgg ctattctgac tatcattcaa aacctggagt tgacagattc
    17581 ctggttggca gcaggttctg tcagaaattt catctggaat cttttgtcag acaaatcccc
    17641 ttttgatcat gaaacagata tagatgtgat tttctttgat ccagattttt ctgtatgagg
    17701 aaaccttatt actggagaaa aagctgagag aggattttcc tcagtaccag tgggaattga
    17761 aaaatcaagt ctatatgcac cagcacagcc ctcatactgc tccctatacc agttcccgtg
    17821 atgctatgag taagtatcca gaacgatgta cggctgttgg actgcgcttg aatgaagaat
    17881 ccgctttgga actctttact ccatacggtc tggaggatat tttgaatttt caagttcgtc
    17941 caactcctca ttttttagag aatcaagaac gaatggctct ctataaaaca cgtttatcca
    18001 agaaaaattg gcaggagaaa tggaaaaatt tgatttttaa aaatacttaa ggaaacttta
    18061 agctagggag tgtacactaa gttcataagt taagaagacc ttaacttaaa ctcctaaaac
    18121 tttttcataa taatctccct ataaaaataa agtcgcccaa tcaggcggct tatttttttg
    18181 aaaaatgggt atggtgcctg agaataaata gcttagtgat agaagaaaat ggggaaatat
    18241 ggtataatga aacgatagat ttttgaatag gaataagatc atgtttggat tttttaagaa
    18301 agataaggct gtggaagtag aggttccgac acaggttcct gctcatatcg gcatcatcat
    18361 ggatggcaat ggccgttggg ctaaaaaacg tatgcaaccg cgagtttttg gacataaggc
    18421 gggcatggaa gcattgcaaa ccgtgaccaa ggcagccaac aaactgggcg tcaaggttat
    18481 tacggtctat gctttttcta cggaaaactg gacccgtcca gatcaggaag tcaagtttat
    18541 catgaacttg ccagtagagt tttatgataa ttatgtcccg gaactacatg cgaataatgt
    18601 taagattcaa atgattgggg agacagaccg cctgcctaag caaacctttg aagctttaac
    18661 caaggctgag gaattgacta agaacaacac aggattgatt cttaattttg ctcttaacta
    18721 cggtggacgt gctgagatta cacaggcgct taagttgatt tcccaggatg ttttagatgc
    18781 caaaatcaac ccaggtgaca tcacagagga attgattggt aactatctct ttactcagca
    18841 tttgcctaag gacttacgag acccagactt gattatccgt actagtggag aattacgttt
    18901 gagcaatttc cttccatggc agggagccta tagtgagctt tattttacgg acaccttatg
    18961 gcctgatttt gacgaagcgg ccttgcagga agctattctt gcctataatc gtcgtcatcg
    19021 ccgatttgga ggagtttagg aggaaatatg acccaggatt tacagaaaag aaccttgttt
    19081 gcagggattg ccctggctat tttcctacca attttaatga ttgggggcct cttgcttcag
    19141 atagcaatcg gaatcatagc catgctagcc atgcatgaac ttttgaagat gagaggtcta
    19201 gagaccatga cgatggaggg cctcttgacc ctctttgcaa cctttgtatt gaccattccc
    19261 ctggaaaatt acctgacttt tttgccagtt gatgggaatg tggttgccta tagtgttttg
    19321 atttcaatca tgttaggaac gaccgttttt agcaagtctt atacgattga ggatgcggtt
    19381 ttccctcttg ctatgagctt ctacgtgggc tttggattta atgctttact agatgctcgt
    19441 gttgcaggtt tggacaaggc tctcttagcc ttgtgtatcg tctgggcgac agacagtggt
    19501 gcctatcttg ttgggatgaa ctatgggaaa cgaaagttag caccaagggt atcgcctaat
    19561 aaaacccttg agggtgcctt gggtggtatt ttaggagcaa ttttagtaac cattatcttt
    19621 atgatagttg acagtacagt tgctcttcca tatggaattt acaagatgtc agtctttgct
    19681 attttcttta gcattgctgg acaatttggt gatttactag aaagttcgat caaacgtcat
    19741 tttggtgtta aggattctgg gaaatttatc cctggacatg gtggtgtttt ggatcgtttc
    19801 gatagtatgt tgcttgtatt tccaatcatg cacttatttg gactctttta atcaaaagac
    19861 ggaggaaacg ctatgctcgg aattttaacc tttattctgg ttttcgggat tattgtagtg
    19921 gtgcacgagt tcgggcactt ctactttgcc aagaaatcag ggattttagt acgtgaattt
    19981 gccatcggta tgggacctaa aatctttgct cacattggca aggatggaac tgcctatacc
    20041 attcgaatct tgcctctggg tggctatgtc cgcatggccg gttggggtga tgatacaact
    20101 gaaatcaaga caggaacgcc tgttagtttg acacttgctg atgatggtaa ggttaaacgc
    20161 atcaatctct caggtaaaaa attggatcaa acagccctcc ctatgcaggt gacccagttt
    20221 gattttgaag acaagctctt tatcaaagga ttggttctgg aagaagaaaa aacatttgca
    20281 gtggatcacg atgcaacggt tgtggaagca gatggtactg aggttcggat tgcaccttta
    20341 gatgttcaat atcaaaatgc gactatctgg ggcaaactga ttaccaattt tgcaggtcct
    20401 atgaacaatt ttatcttagg tgtcgttgtt ttttgggttt taatctttat gcagggtggt
    20461 gtcagagatg ttgataccaa tcagttccat atcatgcccc aaggtgcctt ggccaaggta
    20521 ggagtaccag aaacggcaca aattaccaag attggctcac atgaggttag caactgggaa
    20581 agcttgatcc aagctgtgga aacagaaacc aaagataaga cggcaccgac tttggatgtg
    20641 actatttctg aaaaggggag tgacaaacaa gtcactgtta cacccgaaga tagtcaaggt
    20701 cgttaccttc taggtgttca accgggggtt aagtcagatt ttctatccat gtttgtaggt
    20761 ggttttacaa ctgctgctga ctcagctctc cgaattctct cagctctgaa aaatctgatt
    20821 ttccaaccgg atttgaacaa gttgggtgga cctgttgcta tctttaaggc aagtagtgat
    20881 gctgctaaaa atggaattga gagtatcttg tacttcttgg caatgatttc catcaatatt
    20941 gggattttta atcttattcc gattccagcc ttggatggtg gtaagattgt gctcaatatc
    21001 ctagaagcca tccgccgcaa accattgaaa caagaaattg aaacctatgt caccttggcc
    21061 ggagtggtca tcatggttgt cttgatgatt gctgtgactt ggaatgacat tatgcgactc
    21121 ttttttagat aatcgaggaa tattatgaaa caaagtaaaa tgcctatccc aacgcttcgc
    21181 gaaatgccaa gcgatgctca agttatcagc catgctctta tgttgcgtgc tggttatgtt
    21241 cgccaagttt cagcaggtgt ttattcttat ctaccacttg ccaaccgtgt gattgaaaaa
    21301 gctaaaaaca tcatgcgcca agaattcgaa aagattggtg ctgttgagat gttggctcct
    21361 gcccttctta gtgcagaatt gtggcgtgaa tcaggtcgtt acgaaaccta tggtgaagac
    21421 ctttacaaac taaaaaaccg tgaaaaatca gactttatct taggtccaac tcacgaagaa
    21481 acctttacag ctattgtccg tgattctgtt aaatcttaca agcaattgcc actcaacctt
    21541 tatcaaattc agcccaagta tcgtgatgaa aaacgcccac gtaatggact tcttcgtaca
    21601 cgtgagttta tcatgaagga tgcttatagt ttccacgcta actatgatag tttggatagt
    21661 gtttatgatg agtacaaagc agcctatgag cgtattttca ctcgtagtgg tttagacttc
    21721 aaggctatta ttggtgacgg tggagccatg ggtggtaagg atagccaaga atttatggcc
    21781 attacatctg ctcgtacaga ccttgaccgc tgggttgtct tggacaagtc agttgcctca
    21841 tttgacgaaa ttcctgcaga agtgcaagaa gaaatcaagg cagaattgct caaatggata
    21901 gtctctggtg aagataccat tgcttactca agtgagtcta gctatgcagc taacttagaa
    21961 atggcaacaa acgagtacaa accaagcaac cgtgttgtcg ctgaagaaga agttactcgt
    22021 gttgcaacgc cagatgttaa atcaattgat gaagttgcag ccttcctcaa tgttccagaa
    22081 gaacaaacga ttaaaaccct cttctacatt gcagatggcg agcttgttgc agcccttcta
    22141 gttggaaatg accaactcaa cgaagtcaag ttgaaaaatc acttgggagc agatttcttt
    22201 gacgttgcta gcgaagaaga agtggcgaat gttgttcaag caggatttgg ttcacttgga
    22261 ccagttggtt tgccagagaa tattaaaatt attgcagatc gtaaggtgca agatgttcgc
    22321 aatgcagttg tcggtgctaa cgaagatggc taccacttga ctggtgtgaa cccaggccgt
    22381 gattttactt cagaatatgt ggatatccgt gaagttcgtg agggtgaaat ttccccagat
    22441 ggacaaggtg tccttaattt tgcgcgtggt attgagatcg gtcatatttt caaactcgga
    22501 actcgctatt cagcaagcat gggagcagat gtcttggatg aaaatggtcg tgctgtgcca
    22561 atcatcatgg gatgttacgg tatcggtgtc agccgtcttc tttcagcagt gatggagcaa
    22621 cacgctcgcc tctttgttaa caaaacgcca aaaggtgaat accgttacgc ttggggaatc
    22681 aatttcccta aagaattggc accatttgat gtgcatttga ttactgtcaa tgtcaaggat
    22741 gaagaagcgc aagccttgac agaaaaactt gaagcaagct tgatgggagc tggttatgaa
    22801 gtcttgacag atgaccgtaa cgaacgtgtc ggtgttaaat ttagcgatag tgacttgatt
    22861 ggtcttccaa tccgtatcac tgttggtaaa aaagcggccg atggcattgt agaagttaag
    22921 atcaaggcaa ctggtgacac cattgaggtt catgcagata acgtgcttga aacgcttgaa
    22981 atcctaagca agaaataaaa actataatca gaagaaaaac aaggaaaaaa tgtaactagt
    23041 ttttaccttg ttttttctgt ataatgggag aaatgagtag ataaagaggt aaatgtatgc
    23101 taagatttcc aaaggatttt gtctggggat cctctacttc tggaccgcag acagaaggac
    23161 gtgtagctgg tgacggtaag ggagacaatc tctgggatta ctggttccaa gtggagccaa
    23221 atcgttacta taatgggatt ggttcagata agacatcgac tttttatgaa aattgggagc
    23281 gggatattga gcttttgtta gagactggtc acacagcctt tcggacttct attcagtggt
    23341 cacggatttt tccacaaggc tgtggaaaag tcaaccctca aggtgtggat ttttatcgta
    23401 aggtctttga ggctattaag gctaaaggaa ttcgtctgtt agtcaatctc tatcattttg
    23461 atttaccttt tgcccttcaa gaggatggtg atggttggga aaataaggcg acagtctcag
    23521 cctatgaaga ctatgctcgt ttttgttttg agacttatgg agatttagtg gatcaatgga
    23581 ttacctttaa cgagcccatc gttcctgtag aatttggtta tttttatgat gcccattatc
    23641 cacataaggt ggatgcagag gcagctgtta aggtagccta tcatacacaa ttggccagca
    23701 gccgggctgt taaggcttgc catgaacttt tgcctgattc caagattggg attgtcctca
    23761 acttgacacc ggcttatcca cgtagccagc atcctgctga tgtcaaggca gctcgtattg
    23821 cggccctttt tcaggcccaa tctttcttag atccatctgt cttggggact tatccacagg
    23881 agttggtaga aatcttgcat gaacacggtc ttttacctga tgctacagag gaagagttgg
    23941 aactcattcg tgataatacg gtggacttcc ttggtgtgaa ctactatcaa cctttgcgtg
    24001 ttatggctcc tcgatttgct aagcatccag atagtccact cttaccagaa catttttacg
    24061 agccttatgt gatgcctgga cgtaaaatca attctcaccg tggttgggag atttatgagc
    24121 aagggattta tgacatcgcc caaaatatca aggaaaatta tggcaatatt gaatggatgt
    24181 tgactgagaa tggtatgggt gttgaagggg aagaaaaatt ccgtcaagat ggaatgattc
    24241 aagatgatta ccgtattgac tttgtaaaag gtcatcttcg tgaacttcac cgtgccattg
    24301 aagatggtgc caactgtaag ggctacttga tttggacctt tattgactgc tggtcatggc
    24361 tcaacagcta taaaaatcgc tatggtttgg tcgaattaga cttggaaacg caagaacgtc
    24421 gtctgaagaa atcaggccac tggttcaaag aattaagcga taataatgga ttttaaaaag
    24481 gactctgact gattatccca atcggtcaga gttttttgct tacaggagct taatttgaac
    24541 tataccaatt tttactcttg acaagtgaaa gaaacaagta tatactgttt ttgctgattc
    24601 tataaaagat gaattagact ataccaattt taaggagaaa gcacagcttg cctgtgtcgt
    24661 atatactatg tgtggaattg ttggtgttgt tggaaacaca aatgcaactg atattttgat
    24721 tcaagggctt gaaaagcttg aataccgtgg ctatgattct gtgggaattt ttgtcctaga
    24781 tggtgctgat aaccatttgg tgaaggcggt tggtcgtatt gcagaattgt ctgccaagac
    24841 agctggtgtt gagggaacaa ctggtatcgg acatactcgt tgggcaactc atgggaaacc
    24901 aacggaagac aatgctcacc cacaccgctc tgagacagaa cgttttgtct tggttcacaa
    24961 tggggtgatt gaaaactacc ttgaaattaa agaagaatac cttgctgggc accacttcaa
    25021 agggcaaaca gatacggaaa tcgccgttca tttgattgga aaatttgcgg aagaagaagg
    25081 gctctcagtt cttgaagcct tcaaaaaagc ccttcacatc atccgtggtt cttatgcctt
    25141 tgccttggtt gactcacaag atcctgaagt catctacgta gctaaaaata aatcaccact
    25201 tttgattggt cttggagaag gctataacat ggtctgctca gacgccatgg ctatgattcg
    25261 tgaaactaac caatacatgg aaatccatga ccaagagttg gtaatcgtca aggctgatag
    25321 tgtggaagtt caagactatg atggaaatcg tcgtgagcgt gctagctaca ctgctgaact
    25381 tgacttgtca gatatcggta agggaactta cccttactac atgctcaagg aaattgacga
    25441 gcaaccaact gttatgcgga aactcatcca agcctacaca gatgaggctg gtcaagttgt
    25501 cgtagaccca gatatcatta aggctgttca agacgcagac cgcatctaca tccttgcagc
    25561 tggaacatct taccatgcag gatttgcttc taagaaaatg ttggaagaat tgacagatac
    25621 accagttgaa cttggaatct catctgagtg gggctacggt atgccacttc tcagcaagaa
    25681 accactcttc atctttatca gccaatctgg tgaaacagcg gatagtcgtc aagttttggt
    25741 caaggctaat gaaatgggga ttccaagctt aacagtgaca aacgttccag gttcaaccct
    25801 ctcacgtgaa gccaactata ccatgctcct tcacgcaggt cctgaaattg ccgtggcatc
    25861 aactaaagcc tatacagcgc aaatcgcagc ccttgccttc cttgcaaaag cagtcggaga
    25921 agcaaatggt aatgctaaag cgcaagcctt tgacctggtt catgaattgt caatcgtagc
    25981 tcagtctatc gaatcaactc tttcagagaa agaaaccatt gaagccaagg ttcgtgaact
    26041 tcttgaaaca actcgtaacg ccttttacat cggacgtggt caagattact acgtagccat
    26101 ggaagcaagt ctcaaactca aagagatttc ttatatccag tgtgaaggtt ttgcggcagg
    26161 agaactcaag cacggaacca ttgccttgat tgaagaagga acgcctgtct tgtctctctt
    26221 gtcagatcca gttcttgcca accatactcg tggaaatatc caagaggtcg cagcccgtgg
    26281 tgccaaagtc ctcactatcg cagaagagaa tgtagcaaaa gataccgacg atatcgtcct
    26341 tacgaccgta cattcatacc tctcaccaat ttcaatggtc gtaccaacgc aattagtcgc
    26401 ttactttgca accctccacc gtggcctcga tgtggataaa ccacgtaacc ttgctaaatc
    26461 agtaacggta gaataagcta aaaaagtcta gttatctagg ctttttctaa tgagcaaatt
    26521 ggttgcttgt acttaagatt cgtgttagaa taatttttca aattgaagga cagagataga
    26581 caaggagaat cacaatggta gaattgggaa tttcaacatt tggggaaata acggagcttg
    26641 aagggactgg acaaacttac agtcatgctg aacgcattcg ccagttggtg gcagagattg
    26701 agctggctga caaggttggt ttggacgtat atgggattgg cgagcaccat cgggcggatt
    26761 ttgcagtatc agccccagag attgtcctgg ccgctggggc agtcaatacc aagaaaatcc
    26821 gtttgaccag tgcagtcagc attctgtcaa gcatggaccc gattcgtttg ttccaacagt
    26881 atgccactat cgatgctttg tcaaatggac gatcggagat tatggctgga aggggctctt
    26941 tcacggaatc tttccctctg tttggttatg acttgaaaga ctacgactct ctttttgatg
    27001 agaaattaga cttgcttcag ttagtcaatg aaaagaccaa gttagactgg caaggtcgat
    27061 tgactcaaac gattgctggt aaagaagttt atcctcgtcc agttcaggac aaattgccct
    27121 tgtggatagc tacaggtggt catgtcgaat caacagtgaa gattgctcag gctggtctac
    27181 cgattgtata tgctattatt ggtggtaatc cacgttattt taaaaagttg attcaggctt
    27241 atcgtgagat tggaagcgaa gcgggccatg ctgacaaaga cttgaaagtg ggagcccatt
    27301 cttgggggtg gattgcggaa gatggtgagc aggcggtgaa agattatttc catccgacta
    27361 agcaagtggt ggatgctatt tccaaagacc gtccgcactg gcaggaattg cgttatgagc
    27421 aatatttgga gcaggtaggg tcaaatggcg ccatgtttgt tggcaatcca gatcaggtgg
    27481 ccgaaaaatt gattcgcatg atagaggatt taggattgga ccgcttcatg cttcatctac
    27541 cgcttggttc tatgcctcat gaccaggttc tgagagctat tgaactcttc ggaacacaag
    27601 ttgcacccaa agtacgagct tattttgcta tgaaagaggc ttaataaaaa atcctaatca
    27661 agtttattag gacaacaaat attgtacgaa aattaatccc ccgagctata atgcttgggg
    27721 gatttttcta taggagggct agtttagttc tcttttttat tttttagtaa gaaaccgaga
    27781 ccaaggagcg caaggaggct gattcctgca aataggagtt tgttttcgtt tttgattcct
    27841 gtatttggaa gttcagcttg tttatctggg gttgcaggta tattttcctt gctagagttt
    27901 tctaccgatt cgtttcgaac cgcttctttt acagatgatg cttgtgcttc ttgtgctggt
    27961 tgttcagctt gtgaatcagc tgtagcttgg ctaggtttat tttccgcatc agctactttg
    28021 gcatctggtt tagtagaatc aggtctagct tctggagctg ggtcttgatg tgcagggtga
    28081 gaagcttcat tttgatgttc aggcttggaa ggggttgagt ctggtttctc ttctgcagtt
    28141 gactcatggc tagttccatt ttgagagact cgaagaacag tagctgtaag ggcattcaat
    28201 ttcaagcctt tttcagtcca ttcaagtcct ttcgggttgg caattccgac tggtcctgct
    28261 tggttttcat ctgccaaaac ttccgcattt cttagatgtg caaaggcagt tcccaaatta
    28321 aattcgcgag ctttttcatc cgcattgaca aagactgcgt agatatcgcc gtttggagca
    28381 gtgatttggt agccaatcac tacatcctct ttttccacac cattttggcc tgggacagtg
    28441 atgaggtgga cacggtcttt gatatcttga agactcttaa gtcggaaggc atctgtagat
    28501 tgacgaaggg caatcaaacc tttcatatag tcacggctct tgacattttc aggataagct
    28561 ttaccatctg tagccttagt ccagtcaaac ttgttgactg catcactaga atcgtaagag
    28621 tcatggatga agtaaggata gtcaaatggg ttgccgtcct tatcacgcaa caagtgagat
    28681 ttgtttggaa ccttatcctc tgctacagga gttctgtagg ctgggtcacg gaattgtttg
    28741 gtacgtccat attcctggcc agagtggata aacggagttc cttgagctgt caagaccatg
    28801 agatttccaa gtctcaaacg acggtggatt tcagcatagt tctcagcctt gcttgggtct
    28861 tttttgatag actgggcaat gatgtcaaag agggtcaagt tatcatgggc tgcgatgtat
    28921 tggatgacat ctccagggct gtcagcttca aagttagttg gctgagcaat gagattttta
    28981 aagatagtgt taacatcacg cttgccacct gtgataaagg caggttgacc ttcgtttgga
    29041 taaccagatt tgaggttgtt acggatgtca tctgaaaaga cagcgacagt atcggtatgt
    29101 ttcatccaat cttggtcagc agctttagta ggcatgtttt catcaccggc ataggttctc
    29161 caaccttcgc ctaacatgat tagatttgga ttgagggcgc gtgcagcctt gtaagcttct
    29221 tcgatagaag cggcgtcatg gtctcccatc atatcgaaac ggaatccatc cactttgtag
    29281 gtatcaacta ggtatttgat agagtcaact aggagccgtt tggtcatatg gtgggttgtc
    29341 cccaagcgtc caccaccaaa gctagttcga ggtgtgccat cggcatccat aaagtggtag
    29401 tagtttggct ctaggtcttc aaagatatcg actttggctg tgtggttata aacgacatct
    29461 aggatagctc ccataccacg tttgtggatt tcgttgatga ggtttttaaa ttctgcgatt
    29521 cgtttttctg gattcttagg atcgcttgag tacataccag tcaaggagaa gtagttttga
    29581 gggtcatatc cccagttgta gttgctgttg cttgaagcat agtcagacaa gcgttcatgg
    29641 tttttcaatt cattgacaaa gtagtaagac aagactggaa ggagctggat atgggttaca
    29701 cccaagtctt tgagatagtc tagtttttca atgaaggctt caaaagtccc aaatggtttg
    29761 gtcaagtctt ttgcaatggc aggatctgaa gtgaagtcac gcacatgagc ttcgtagata
    29821 acggcgtctt cacgagtctt gaaattgtga atcttaccat aagtcaagtc ttgaggtccg
    29881 agtttagctg gatctacaaa ggcggcttta gccactttat gggcatcgtc aatcttggca
    29941 tcgtcgctat tccaagcagc aagagattta gcgtaaggat cgagtgcaag aacagtttta
    30001 ccttgacgct cgatttggta ttgataatag tagccagtga aatctgtgat tccgagtttg
    30061 tttgtgctgt ctagagtttg tttccaagtt cctctttccc ctttttcgag agcgacagtt
    30121 ccaactactt tgtcagggtc attcttgtcg tagacaacaa cagaaacctt atcagcactt
    30181 ggtgaccaaa gggtcaaatc aacttgtttt ccttcttctt ttaggtcagc tcccagtttg
    30241 ccatcatagc tgtatgtctc atctttcagg cgccagcttg ttttggtagt gaattggtcg
    30301 gaattgtagc taacagtata aggatgtttt gtgtcagaga aatctccgct gtaggtcact
    30361 ttcttaccag cttcatcgat tgcaacatcg gtaatagtta ccttgtttcc taggtgatta
    30421 gtgatgttgg agtgtttgag gatatcttct tttttagcac cgacaagtgt tgaaaagcta
    30481 ctttcaatgc tagaagtgcc tacgtgttgg gctcctgtca tacggatatc atggacatag
    30541 tatggatttg tgtaaatcga ttcatcatcg tcttttagga aaatttggct atgatttttc
    30601 aaatctgtga acttataatt ttctttacgg attttcacgt cgtctccttg tttgctctca
    30661 tctagtaata aaaatccaaa ttctcttgcg gcttcattaa gaggaatgtc gatatagcgg
    30721 ccatatttgc ctgtagccgt aaagtctgtt ccgtcaggcc attgagcgct acttggattt
    30781 tttacatctc cccagtacca gagagatttc ttgtcatagt tgccatctgt gcggtagtag
    30841 ttgacgcgaa cagttcctgc aggctgtggc tcgtaagaga aaaccttgta atcttggtct
    30901 aaccaagcct cgttcatttt tggaactagt ttttctacag atttatcgcc ggttagattt
    30961 tttccagctg tattgttgat gaggaagcta attttcttgg cttgttctcc ctttaatttg
    31021 acatctaggt aatagccgta gtcatctttc ttggcatcct tgaaggacaa agctccgttt
    31081 ggccagtttt cagatggttt ttcaacatcg tcccaagtcc atagtccttg agcatccttg
    31141 ttttcttcag gaagtttttt gacatggata cggaagtagt tatcttcgat gggctcttct
    31201 gccttagttt cagttgttac tggactagta gaagtagttg gctctgttgc aggtttttct
    31261 tctgttttct ctagggaagc gtttgcattt ctaggtgaag aaatatcact ttcgttctta
    31321 ccagagccag ctgctgtatc agtaagaggt tgggcaaggg tggttgtatt tctgttgttg
    31381 ggttcgttag tggttggtgt actttcgtcg gatgagatag ttggtgtagc catggcaagc
    31441 aagacaaggc ttgcgccgat aaggacagaa ccagttccat ttttgaggga acggatgctg
    31501 tagaccattt ttttctcagt gtgagatggt gtttttctca taatgattct ccaatcaata
    31561 aatttctata tcagtatagc atgtagtaaa acaatatgca agcgttttct ttgatttgtg
    31621 aaaaaaattc ttacttttga gaagttttct gtactatttt atatatcagt ataaatgacg
    31681 gagataaaaa tgtcacataa gccaagccgt attattagga agatgatgtg ttcccagcta
    31741 tttttattca gaaattgatc tctaacatta ccaataaaaa agaaaaatat cttgacaagc
    31801 aagggaaaat tttgttacaa taatagacgg tactttttac ttttggtctc tcaagagtgt
    31861 acagggacgt gctgacaaat gttgcaaaag tacacacaga tgatatctgt caccaagtgt
    31921 atcatcacca aaaataaaaa aacacaggag aatgtagatg cctacaatta accaattggt
    31981 tcgcaaaccg cgtaaatcaa aagtagaaaa atctaaatca ccagctttga acgttggtta
    32041 caatagtcat aaaaaagttc aaacaaacgt ttcttcacca caaaaacgtg gtgttgcaac
    32101 tcgtgttgga acaatgacac ctaaaaaacc taactcagcc cttcgtaaat tcgctcgtgt
    32161 acgtttgagc aaccttatcg aagttactgc ctacatccca ggtatcggac acaacttgca
    32221 agagcacagc gtggtgcttc ttcgcggtgg acgtgtaaaa gaccttccag gggtacgtta
    32281 ccatatcgtc cgtggtgcac ttgatactgc aggtgttaac gatcgtaaac aaggccgttc
    32341 taaatacggt actaaacgtc caaaagcata aggaaagggg ataaagagaa atgagtcgta
    32401 aaaatagagc tccaaaacgt gacgtattgc cagatccgct atacaattca caactagtta
    32461 ctcgtcttat caaccgcgtt atgcttgatg gtaaacgtgg tactgctgct tcaatcgttt
    32521 acggtgcttt tgagcaaatc aaagaagcta ctggcaacga tgcacttgaa gtatttgaaa
    32581 cagctatgga aaacatcatg cctgtacttg aagtacgtgc acgtcgtgtt ggtggttcta
    32641 actaccaagt cccagttgaa gttcgtccag aacgtcgtac aacacttgga cttcgttggt
    32701 tggtaacaat cgctcgtctt cgtggtgaac acacaatgca agaccgtctt gcaaaagaaa
    32761 tcttggatgc tgctaacaac actggtgcag ctgttaagaa acgtgaagat actcaccgta
    32821 tggctgaagc taaccgtgca ttcgcacact tccgttggta agataggatg cgaaagcgtt
    32881 aagaaagtcc cagagaaaat agggaatcga agcaggttgc ggttgcaacc aatgagattc
    32941 atctttttct ccagactttt agcttgagct caactaaatc atgatgctag gaacggtaag
    33001 gatgcaaggt gaaaatagga aactgacgca gtattcgacg aatacaagga gttttatctt
    33061 tttcacgcag catcccgttc cagctcacat cggctaacta actttagccc gggttcaaat
    33121 tagctaaatc gattagtatt agctataact cagcttacca tctcgtaagt tgaaaccaac
    33181 aatagcatga aaacattgag aacgggtagg tcctgcctat ccgtttttat taaaatcgtg
    33241 ttataataga atagaaatca aaaataaata ggagaaacaa acctcatggc acgcgaattt
    33301 tcacttgaaa aaactcgtaa tatcggtatc atggctcacg tcgatgccgg taaaacaaca
    33361 actactgagc gtattcttta ctacactggt aaaatccaca aaatcggtga aactcacgaa
    33421 ggtgcgtcac aaatggactg gatggagcaa gagcaagaac gtggtatcac gatcacatct
    33481 gctgcgacaa cagctcaatg gaacaaccac cgcgtaaaca tcatcgacac accaggacac
    33541 gtggacttca caatcgaagt acaacgttct cttcgtgtat tggatggtgc ggttaccgtt
    33601 cttgactcac aatcaggtgt tgagcctcaa actgaaacag tttggcgtca agcaactgag
    33661 tacggagttc cacgtatcgt atttgccaac aaaatggaca aaatcggtgc tgacttcctt
    33721 tactctgtaa gcacacttca cgatcgtctt caagcaaatg cacacccaat ccaattgcca
    33781 atcggttctg aagatgactt ccgtggtatc attgacttga tcaagatgaa agctgaaatc
    33841 tatactaacg accttggtac ggatatcctt gaagaagaca tcccagctga ataccttgac
    33901 caagctcaag aataccgtga aaaattgatt gaagcagttg ctgaaactga cgaagaattg
    33961 atgatgaaat acctcgaagg tgaagaaatc actaacgaag aattgaaagc tggtatccgt
    34021 aaagcgacta tcaacgttga attcttccca gtattgtgtg gttcagcctt caaaaacaaa
    34081 ggtgttcaat tgatgcttga tgcggttatc gactaccttc caagcccact tgacatccca
    34141 gcaatcaaag gtattaaccc agatacagac gctgaagaaa ttcgtccagc atctgacgaa
    34201 gagccatttg cagctcttgc cttcaagatc atgactgacc cattcgtagg tcgtttgaca
    34261 ttcttccgtg tttactcagg tgttcttcaa tcaggttcat acgtattgaa tacttctaaa
    34321 ggtaaacgtg aacgtatcgg acgtatcctt caaatgcacg ctaacagccg tcaagaaatc
    34381 gacactgttt actcaggtga tatcgctgct gccgttggtt tgaaagatac tacaactggt
    34441 gactcattga cagatgaaaa agctaaaatc atccttgagt caatcaacgt tccagaacca
    34501 gttatccaat tgatggttga gccaaaatct aaagctgacc aagacaagat gggtatcgcc
    34561 cttcaaaaat tggctgaaga agatccaaca ttccgcgttg aaacaaacgt tgaaactggt
    34621 gaaacagtta tctcaggtat gggtgaactt caccttgacg tccttgttga tcgtatgcgt
    34681 cgtgagttca aagttgaagc gaacgtaggt gctcctcaag tatcttaccg tgaaacattc
    34741 cgcgcttcta ctcaagcacg cggattcttc aaacgtcagt ctggtggtaa aggtcaattc
    34801 ggtgatgtat ggattgaatt tactccaaac gaagaaggta aaggattcga attcgaaaac
    34861 gcaatcgtcg gtggtgtggt tcctcgtgaa tttatcccag cggttgaaaa aggtttggta
    34921 gaatctatgg ctaacggtgt tcttgcaggt tacccaatgg ttgacgttaa agctaagctt
    34981 tatgatggtt catatcacga tgtcgactca tctgaaactg ccttcaagat tgcggcttca
    35041 ctttccctta aagaagctgc taaatcagca caaccagcta tccttgaacc aatgatgctt
    35101 gtaacaatca ctgttccaga agaaaacctt ggtgatgtta tgggtcacgt aactgctcgt
    35161 cgtggacgtg tagatggtat ggaagcacac ggtaacagcc aaatcgttcg tgcttacgtt
    35221 ccacttgctg aaatgttcgg ttacgcaaca gttcttcgtt ctgcatctca aggacgtggt
    35281 acattcatga tggtatttga ccactacgaa gatgtaccta agtcagtaca agaagaaatt
    35341 attaagaaaa ataaaggtga agactaatcc gtcctcactc tagaaggaag tcacttagtg
    35401 gcttcctttt gtctttagaa aatacctcta aatatggtaa aatagtagaa gaataatgtg
    35461 aggaaaatga atgtcaaata gttttgaaat tttgatgaat caattgggga tgcctgctga
    35521 aatgagacag gctcctgctt tagcacaggc caatattgag cgagttgtgg ttcataaaat
    35581 tagtaaggta tgggagtttc atttcgtatt ttctaatatt ttaccgattg aaatcttttt
    35641 agaattaaag aaaggtttga gcgaagaatt ttctaagaca ggcaataaag ctgtttttga
    35701 aattaaggct cggtctcaag aattttcaaa tcagctcttg cagtcctact atagggaagc
    35761 tttctctgaa ggtccatgtg ctagtcaagg ttttaagtcc ctttatcaaa atttgcaagt
    35821 tcgtgctgag ggtaatcagc tatttattga aggatctgaa gcgattgata aggaacattt
    35881 taagaagaat catcttccta atttagccaa acaacttgaa aagtttggtt ttccaacttt
    35941 taactgtcaa gtcgagaaga atgatgtcct gacccaagag caggaagagg cctttcatgc
    36001 tgaaaatgag cagattgttc aagctgccaa tgaggaagcg ctccgtgcta tggaacaact
    36061 ggagcagatg gcacctcctc cagcggaaga gaaaccagtc tttgattttc aagcgaaaaa
    36121 agctgcagct aaacccaagc tggataaggc ggagattact cctatgatcg aagtgacgac
    36181 agaggaaaat cgtctggtat ttgaaggggt tgtttttgat gtggagcaaa aagtgactag
    36241 aacaggtcgt gttttaatca actttaaaat gacggactat acttcaagtt tttctatgca
    36301 aaagtgggtt aaaaacgagg aagaggccca gaagtttgac ctcatcaaga agaattcttg
    36361 gctccgagtt cgagggaatg tggagatgaa taacttcaca cgcgatttga ctatgaacgt
    36421 acaggatctg caggaagttg ttcactatga gcggaaggat ttgatgccag aaggtgagcg
    36481 tcgggttgag tttcatgctc atactaacat gtcgactatg gatgctttgc cagaggtcga
    36541 agagattgtt gcaacagctg ctaagtgggg acacaaggcg gttgctatca cagaccatgg
    36601 gaatgtccag tcctttccac atggctataa ggcggctaag aaagcgggaa tccagctgat
    36661 ctatgggatg gaagccaata tcgtggagga ccgtgtccct atcgtctata acgaagtgga
    36721 gatggactta tcagaagcaa cctacgtggt ctttgacgtg gaaacgacgg gactttcagc
    36781 tatctataat gacttgattc aggttgcggc ctctaagatg tacaagggga atgttattgc
    36841 tgaatttgat gaatttatca atcctgggca tcccttgtca gcttttacta cagagttaac
    36901 tggaattaca gatgatcatg tcaaaaatgc caaaccacta gaacaagttt tgcaagaatt
    36961 ccaagaattt tgcaaggata cggtcctagt tgcccacaat gctacctttg acgttggctt
    37021 tatgaatgct aattatgagc gtcatgatct tccaaagatt agtcagccag ttattgatac
    37081 gctggagttt gctagaaacc tctatcctga gtataaacgc catggtttgg ggcctttgac
    37141 caagcgtttt ggtgtggcct tggaacatca ccacatggcc aactacgatg cggaagcgac
    37201 tggtcgtctg cttttcatct ttatcaaaga ggtagcagaa aaacatggtg tgaccgattt
    37261 agctagactc aacattgatc taatcagtcc agattcttac aaaaaagctc ggatcaagca
    37321 tgcgaccatc tatgtcaaga atcaggtagg tctaaaaaat atctttaagc tggtttcctt
    37381 gtctaatacc aagtattttg aaggagtgtc acggattccg agaacggttc tagatgccca
    37441 tcgagagggc ttgattttag gttcagcctg ttcagagggt gaagtttttg acgtggtcgt
    37501 ttctcaaggt gtggatgcgg cggttgaggt ggccaagtat tatgacttta tcgaggtcat
    37561 gccaccggct atctatgcgc ccttgattgc taaagagcag gtcaaggata tggaggaact
    37621 ccagaccatt atcaagagtt tgatagaggt tggagaccgc cttggcaagc ctgttttggc
    37681 tacgggaaat gttcactata tcgaaccgga agaagagatt tatcgtgaaa ttatcgtccg
    37741 tagtttggga cagggtgcga tgattaaccg aactatcggt catggtgaac atgcccaacc
    37801 agcaccactt ccaaaggctc attttcgaac gactaatgag atgttggatg aatttgcctt
    37861 tttgggagag gaactggctc gtaaactggt tattgaaaac accaatgcct tggcagaaat
    37921 atttgaaccc gttgaagtcg ttaagggtga cttgtatacg cctttcatcg acaaggctga
    37981 agaaacagtt gctgagttga cctataagaa agcttttgag atttatggaa atccgctgcc
    38041 agatattgtt gatttgcgga ttgaaaaaga attaacatcc atactgggga atggatttgc
    38101 tgtgatttat ctggcatcgc agatgctggt gcaacgttct aatgaacggg gttatttggt
    38161 tggttctcgt gggtctgtcg gatctagttt cgttgcgacc atgattggga ttacggaggt
    38221 caatcctctc tctcctcact atgtctgtgg tcagtgtcag tacagtgagt ttatcacaga
    38281 tggttcgtac ggttcaggat ttgatatgcc ccataaggac tgtccaaact gtggtcacaa
    38341 actcagtaaa aacggacagg atattccgtt tgagaccttc cttggctttg atggggataa
    38401 ggttcctgat attgacttga acttctcggg agaagatcag cctagcgccc acttggatgt
    38461 gcgtgatatc tttggtgaag aatatgcctt ccgtgcggga acagttggta cggtagctgc
    38521 caagactgcc tatggatttg tcaagggtta cgagcgagat tatggcaagt tttatcgtga
    38581 tgcagaagta gaacgcctcg ctcaaggagc ggcgggtgtc aagcggacaa caggccaaca
    38641 cccgggggga atcgttgtta ttccgaacta catggatgtc tacgatttta cgcctgtcca
    38701 gtatccagca gatgatgtca cggctgaatg gcagaccact cactttaact tccacgatat
    38761 cgatgagaac gtcctcaaac tcgatgtact gggacatgat gatccgacca tgattcgaaa
    38821 acttcaggat ttgtctggta ttgaccctaa taaaattcct atggatgacg aaggcgtgat
    38881 ggcactcttt tctgggactg atgtgctagg ggtaacacct gaacaaattg gaacgcctac
    38941 gggtatgttg gggattccag agtttggaac aaatttcgta cgtggaatgg tagacgaaac
    39001 ccatccgaca acctttgcgg aattgcttca gctgtctggt ctgtcccacg gtactgatgt
    39061 ttggttgggg aatgctcagg atctgattaa gcaaggaata gcggacctat cgactgttat
    39121 cggttgtcgg gacgacatca tggtttacct catgcatgcg ggtctggaac ctaagatggc
    39181 ctttactatt atggaacggg tacgtaaggg tttgtggcta aagatttcag aagaggagag
    39241 aaatggctat atcgaagcaa tgaaggctaa taaggtgcca gagtggtata tcgaatcctg
    39301 tgggaaaatt aagtacatgt tccctaaggc ccatgcggca gcctacgtta tgatggcctt
    39361 gcgtgtagct tacttcaagg ttcaccatcc tatttattac tactgtgctt acttctccat
    39421 tcgtgctaag gcttttgata tcaagaccat gggtgcgggc ttggaggtca tcaagcgcag
    39481 aatggaagaa atctctgaaa aacggaagaa caatgaagcc tctaatgtgg aaatcgatct
    39541 ctatacaact cttgagattg tcaatgagat gtgggaacga ggtttcaagt ttggtaaatt
    39601 agatctctac tgtagtcagg cgacagagtt cctcatcgac ggggataccc ttatcccacc
    39661 atttgtagca atggatggtc tgggagagaa cgttgccaag caactggtgc gggcgcgtga
    39721 agagggagaa ttcctctcta aaacagaact acgcaagcgt ggtggactct catcaacctt
    39781 ggttgaaaag atggatgaga tgggtattct tggaaatatg ccagaggata accagttgag
    39841 tttgtttgat gagttgtttt aaaaaattgc ttaataatct attaaaagag gctaacgtat
    39901 atccaataga tttacattag ctttcttttt tgttaaaata gtctatggaa agagggtgag
    39961 agtatgtcaa agatgagtat aagcatccgt ctggatagtg aggttaagga gcaggcccaa
    40021 caggtgttta gtaatctggg aatggatatg acaacagcta ttaatatttt ccttcgtcag
    40081 gcaattcaat atcagggatt accttttgat gttagactag acgaaaatcg gaagttgctc
    40141 caagtgttaa cggatttaga ccaaaatcgt aatatgagcc agtcttttga atcagtctca
    40201 gatttgatgg aggacttacg tgcttaagat tcgttatcat aaacagttta aaaaagattt
    40261 taagttggct atgaagcgtg gtttgaaggc agaattatta gaagaagttt tgaattttct
    40321 ggttcaagaa aaagaacatc ctgccagata tcgtgatcat tcattgacgg catccaagca
    40381 ttttcaagga gttcgtgaat gccataccca gccagattgg cttttggttt ataaagtaga
    40441 caagtcggaa ttgattttaa atttgctgag gacaggcagt cacagtgatt tattttaatc
    40501 tattttaagg gggttctcat gaaactaaga atatttgcgg aagataagcc ggctaagaag
    40561 gtatttgaat atcaattaga acttgctgat cgtacaattc ttctatcgac agcactcttg
    40621 tcaggtgcta ttgctttagc aggaatcttt tctgctttga aagaaaaata aaaatagaaa
    40681 agagaaaaca gaatggtttt accaaatttt aaagaaaatc tagaaaaata tgcgaaattg
    40741 ttggttgcga acggaattaa cgtgcaacct ggtcacactt tggctctctc tattgatgtg
    40801 gagcaacgtg aattggcaca tctaatcgtg aaagaagctt atgccttggg tgcgcatgag
    40861 gtcatcgttc agtggacaga tgatgtgatt aaccgtgaga aattcctcca tgccccgatg
    40921 gagcgtttgg acaatgtgcc agaatacaag attgctgaga tgaactatct cttggagaac
    40981 aaggctagcc gtcttggagt tcgttcatct gatccaggtg ccttgaacgg agtggacgct
    41041 gacaagcttt cagcttctgc taaagctatg ggacttgcca tgaagcctat gcgtatcgca
    41101 actcaatcta acaaggttag ctggactgta gcagctgcag caggacttga gtgggctaag
    41161 aaagtcttcc caaatgctgc gagcgacgaa gaagcagttg atttcctttg ggaccaaatt
    41221 ttcaaaactt gccgtgtcta cgaagcagct cctgttaagg cttgggagga acatgcagcc
    41281 attctcaaga gcaaggccaa tatgcttaat aaggagcaat tttcagccct tcactacaca
    41341 gcgccaggaa cagatttaac acttggtttg ccaaagaacc acgtttggga atcagctggt
    41401 gctgtcaatg cacagggcga agaattcttg ccaaatatgc caacagaaga ggtcttcaca
    41461 gcgcctgact tccgtcgtgc agatggttat gtcacttcta caaaaccgct tagctacaac
    41521 ggaaatatca ttgaaggcat taaggtgacc tttaaggatg gacaaatcgt agatatcact
    41581 gctgagaagg gtgatcaggt tatgaaagac cttgtctttg aaaatgcggg tgcgcgtgcc
    41641 ttgggtgaat gtgccttggt accagatcca agtccaattt ctcagtcagg cattaccttc
    41701 tttaacaccc ttttcgatga aaatgcgtca aaccacttgg ctatcggtgc agcctatgcg
    41761 actagcgttg ttgatggagc ggagatgagc gaagaggagc ttgaagctgc agggcttaac
    41821 cgttcagatg ttcacgtaga ctttatgatt ggttctaacc aaatggatat cgatggtatt
    41881 cgtgaggatg gaacgcgagt acctcttttc cgtaatggga attgggcaaa ttaaggagat
    41941 aatatgttag gaagtatgtt cgttggtctc ttagtgggat ttttagcagg tgctatgacc
    42001 aatcgtggag agcgaatggg atgttttgga aaaatgtttc tcggttggat cggagccttt
    42061 ctaggtcact tgctctttgg aacttggggg ccagttttat caggaacagc tattatccca
    42121 gcgattttag gagccatgat tgttttagct attttttgga gacgaggaag ttaatttcct
    42181 aaatctgata ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggttgctcaa
    42241 agcactgctt tgaggttgca gatagaactg acgaagtcag taacatatat acggcaaggc
    42301 gacgttgacg cggtttgaag agatttttga agagtatgaa acgaaaagga ggttggtcat
    42361 tgtactagcc tccttttgat atgatataat agttctatga gattagataa atttttagtt
    42421 gcctgtgcgg tgggaagtcg gactgaggtc aaaaacttgc tcaaggctgg gcgcgtgact
    42481 gtaaatggta aaaaagaaaa atcagctaaa ttgcagattg atgaaaaaat agatgagatt
    42541 cgctttgatg ggcaagtgtt ggagtatgaa gagtttgtct actacatgat gaacaagccc
    42601 aaaggagtta tctcagcgac tgaggatccc aagcacagaa ccgttctgga cttgttggat
    42661 gacttggcgc ggagtaagga agttttccca gtaggacgct tggatattga cacgcatggt
    42721 cttttgctct tgaccaatga tggtcagctg gctcatgttc ttctttcgcc caagcgtcat
    42781 gtggacaaga cttatctggc acaagtcaag ggaatcatga cccaagaaga tgtggagaca
    42841 tttgctgagg gtattcctct caaagacttt acctgtcaac ccgctatact ggagcttgta
    42901 tccatagata cagaaaagaa tcaaagccaa atccgtgtga ccattgcaga agggaagttt
    42961 catcagatca agcgtatggt gggctactgt ggcaaggaag tggtggactt gcaacgattg
    43021 actatgggaa ctttagtatt agatgagaac ctggaacgag gggaatggcg tcgcttgacc
    43081 aaggaagaat tagaaattct ccgcgctaat attatttaat ttagttt
//
LOCUS       DAVKJU010000010        42871 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVKJU010000010 DAVKJU010000000
VERSION     DAVKJU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.010"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(33..1262)
                     /locus_tag="VW191_000444"
     CDS             complement(33..1262)
                     /locus_tag="VW191_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HEU9623275.1"
                     /translation="MKITIKRYSLPEILGLAALAIFLLFSLLDVSFYVQYISPMLYKA
                     AIFLIIMLIILKEFVSGSNSFESLLGLLGVSTLYFIIGSVTSYTSFIVLGLSLIYSLR
                     DIPFSKVVKVAFIISVAMLFLVILSSKIGFIPDYIEFSSTRVRHYLGFRYSLFPSTIM
                     MNIIAISFFLKQETVSYQRLFILFLVSMYLYLETDSRLTFISSSLLLLANLAMKLIPT
                     VIEKFSLLLKFFTLTYFVNAYLSYWISKNYLRTSNALLNHFFHQADQFLGGRIYLSNR
                     SLSLYGYGILGQKIAWVGNALNAQGERSTDAYLYVDNLYIQILQHFGLIALIIILSLL
                     TATLVKLLRKGQIVLSIIIVSLSFHALIDDLILSIHYNIFWILLGSLIYSNYQFSEER
                     YGEVENDSLRRTVREGY"
     gene            complement(1271..2077)
                     /locus_tag="VW191_000445"
     CDS             complement(1271..2077)
                     /locus_tag="VW191_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HEU9623276.1"
                     /translation="MERSRLIDVKIIVATHKEVKMPQDNSLYLPIHVGRDGKSDIGFI
                     GDNTGDNISSLNPYYCELTGLYWAWKNLDYNYLGLVHYRRYFTNKSQGYNENVNMDDV
                     ILSRSNVEILLEKSDVIVPKKRKYYIETLYSHYAHTLNGEHLDLARKIIEQNSSEYLS
                     SFDKVMKQRSGYMFNMFIMKKGLLDDYLPWLFSILDTMYEQMDLTDYTSFESRLFGRV
                     SELLFNVWLCKKGITPKEVPFMYMERVDLFEKGKSFLMAKFFGKKYGQSF"
     gene            complement(2064..3431)
                     /locus_tag="VW191_000446"
     CDS             complement(2064..3431)
                     /locus_tag="VW191_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9623277.1"
                     /translation="MNGKILRSSLAIIQSFLVILLTYLLSAVRETEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRRYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYLNFKGSKKILLLTATSHVEKVLDRLIESNEVVGKL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHIMAKRIIDIMGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDDWTIWKDIEILLKTVKVVLM
                     KDGAK"
     gene            complement(3446..4135)
                     /locus_tag="VW191_000447"
     CDS             complement(3446..4135)
                     /locus_tag="VW191_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HEU9623278.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     IGEGKSTTSANIAWAFARAGYKTLLIDGDIRNSVMLGVFKARNKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRNCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGIVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            complement(4145..4837)
                     /locus_tag="VW191_000448"
     CDS             complement(4145..4837)
                     /locus_tag="VW191_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein CpsC"
                     /protein_id="HEU9623279.1"
                     /translation="MKEQNTIEIDVFQLFKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKIK"
     gene            complement(4846..5577)
                     /gene="cps4B"
                     /locus_tag="VW191_000449"
     CDS             complement(4846..5577)
                     /gene="cps4B"
                     /locus_tag="VW191_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HEU9623280.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDRGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHIIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(5579..7024)
                     /gene="cps4A"
                     /locus_tag="VW191_000450"
     CDS             complement(5579..7024)
                     /gene="cps4A"
                     /locus_tag="VW191_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEU9623281.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVGFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLVIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            7249..7771
                     /locus_tag="VW191_000451"
                     /pseudo
     CDS             7249..7771
                     /locus_tag="VW191_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7764..7937)
                     /locus_tag="VW191_000452"
     CDS             complement(7764..7937)
                     /locus_tag="VW191_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEU9623282.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYADNTVN"
     gene            complement(8097..9704)
                     /locus_tag="VW191_000453"
     CDS             complement(8097..9704)
                     /locus_tag="VW191_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU9623283.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGAKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMISVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            9857..11341
                     /locus_tag="VW191_000454"
     CDS             9857..11341
                     /locus_tag="VW191_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEU9623284.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQLAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCLSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGESPYA
                     SPTDMGVNMVGFAITDDEAAIEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVTARQKAEETGGPALALELPTGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            11436..11918
                     /locus_tag="VW191_000455"
     CDS             11436..11918
                     /locus_tag="VW191_000455"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEU9623285.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDVISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAEIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(12088..14193)
                     /locus_tag="VW191_000456"
     CDS             complement(12088..14193)
                     /locus_tag="VW191_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9623286.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNVEVDGKMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     EAEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGPTGVGKTELAKQLALDMFGTKDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKGIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGGLVIREKA"
     gene            complement(14737..15717)
                     /locus_tag="VW191_000457"
     CDS             complement(14737..15717)
                     /locus_tag="VW191_000457"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU9623287.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLIASVLVAFFFALFSNQLSNNVGMILFILVLYGLIGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGIDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFVFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKMTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(15719..17971)
                     /locus_tag="VW191_000458"
     CDS             complement(15719..17971)
                     /locus_tag="VW191_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU9623288.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELETAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     LESSLNTILAGTDGIITYEKDRVGNIVPGTELVSQQTVDGKDVYTTLSSPLQSFMETQ
                     MDAFLEKVKGKYMTATLVSAKTGEILATTQRPTFNADTKEGITEDFVWRDILYQSNYE
                     PGSAMKVMTLASSIDNNTFPSGEYFNSSEFKIADATTRDWDVNEGLTTGGMMTFLQGF
                     AHSSNVGMSLLEQKMGDATWLDYLKRFKFGVPTRFGLTDEYAGQLPADNIVSIAQSSF
                     GQGISVTQTQMLRAFTAIANDGVMLEPKFISAIYDTNNQSVRKSQKEIVGNPVSKEAA
                     STTRNHMILVGTDPLYGTMYNHYTGKPIITVPGQNVAVKSGTAQIADEKNGGYLVGST
                     NYIFSVVTMNPAENPDFILYVTVQQPEHYSGIQLGEFATPILERASAMKESLNLQSPA
                     KNLDKVTTESSYAMPSIKDISPGELAEALRRNIVQPIVVGTGTKIKETSVEEGTNLAP
                     NQQVLLLSDKVEEIPDMYGWKKETAETFAKWLDIELEFEGSGSVVQKQDVRTNTAIKN
                     IKKITLTLGD"
     gene            complement(17975..18292)
                     /gene="ftsL"
                     /locus_tag="VW191_000459"
     CDS             complement(17975..18292)
                     /gene="ftsL"
                     /locus_tag="VW191_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEU9623289.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(18304..19254)
                     /gene="rsmH"
                     /locus_tag="VW191_000460"
     CDS             complement(18304..19254)
                     /gene="rsmH"
                     /locus_tag="VW191_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU9623290.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            19420..19614
                     /locus_tag="VW191_000461"
     CDS             19420..19614
                     /locus_tag="VW191_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9623291.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            19630..20172
                     /locus_tag="VW191_000462"
     CDS             19630..20172
                     /locus_tag="VW191_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEU9623292.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            20183..20425
                     /locus_tag="VW191_000463"
     CDS             20183..20425
                     /locus_tag="VW191_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623293.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            20436..20771
                     /locus_tag="VW191_000464"
     CDS             20436..20771
                     /locus_tag="VW191_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623294.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(20881..21882)
                     /locus_tag="VW191_000465"
     CDS             complement(20881..21882)
                     /locus_tag="VW191_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9623295.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(21942..23843)
                     /locus_tag="VW191_000466"
     CDS             complement(21942..23843)
                     /locus_tag="VW191_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEU9623296.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRILDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSILFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQKLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(23865..24158)
                     /locus_tag="VW191_000467"
     CDS             complement(23865..24158)
                     /locus_tag="VW191_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9623297.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(24158..24976)
                     /locus_tag="VW191_000468"
     CDS             complement(24158..24976)
                     /locus_tag="VW191_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9623298.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(24963..25742)
                     /locus_tag="VW191_000469"
     CDS             complement(24963..25742)
                     /locus_tag="VW191_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9623299.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(25757..26248)
                     /locus_tag="VW191_000470"
     CDS             complement(25757..26248)
                     /locus_tag="VW191_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9623300.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(26259..27449)
                     /locus_tag="VW191_000471"
     CDS             complement(26259..27449)
                     /locus_tag="VW191_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEU9623301.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(27461..27895)
                     /locus_tag="VW191_000472"
     CDS             complement(27461..27895)
                     /locus_tag="VW191_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9623302.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMPADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            28166..28981
                     /locus_tag="VW191_000473"
     CDS             28166..28981
                     /locus_tag="VW191_000473"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEU9623303.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            29000..29641
                     /locus_tag="VW191_000474"
     CDS             29000..29641
                     /locus_tag="VW191_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU9623304.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            29672..30673
                     /locus_tag="VW191_000475"
     CDS             29672..30673
                     /locus_tag="VW191_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEU9623305.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            30683..31312
                     /locus_tag="VW191_000476"
     CDS             30683..31312
                     /locus_tag="VW191_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEU9623306.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSALSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(31691..32076)
                     /gene="tnpA"
                     /locus_tag="VW191_000477"
                     /pseudo
     CDS             complement(31691..32076)
                     /gene="tnpA"
                     /locus_tag="VW191_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(32457..35660)
                     /locus_tag="VW191_000478"
     CDS             complement(32457..35660)
                     /locus_tag="VW191_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEU9623307.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDNKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNREKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLELSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            35883..36359
                     /locus_tag="VW191_000479"
     CDS             35883..36359
                     /locus_tag="VW191_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEU9623308.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINTFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDHKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            complement(36581..38794)
                     /locus_tag="VW191_000480"
     CDS             complement(36581..38794)
                     /locus_tag="VW191_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU9623309.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFATEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(39195..39371)
                     /locus_tag="VW191_000481"
     CDS             complement(39195..39371)
                     /locus_tag="VW191_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623310.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            39518..39940
                     /locus_tag="VW191_000482"
     CDS             39518..39940
                     /locus_tag="VW191_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9623311.1"
                     /translation="MRFIVETFPIFSISNDLLQLLTSWKKQQKYELAKFGIISNPEQF
                     IFTYIDTKGNINKPLHSDYLNNKMKTIRKRHPELTHATPHKLRHTGATLAKQAGMSLE
                     AISEALIHSDTGTTQIYVNTSNVVPMAVGEFALQSLKQ"
     gene            40141..41433
                     /locus_tag="VW191_000483"
     CDS             40141..41433
                     /locus_tag="VW191_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9623312.1"
                     /translation="MLSQYVGRKTSCIENEITKTRNHSRQRGNIMRVVFGIDVSKASS
                     EVAILVNGDKVHGYTIPNDSIGFNRLLGDLKTVSNPEIVFEATGVYSRRLQAFLEDNS
                     YAYTRLNPLAAKKQLDSLRVRKTDKIDAEKLAQSQFVLNRQPTYVQEEVYQELRDLSR
                     FYQNLTEDIVRAKNRLHKVLQVTFPELESLLSSPTGEQYWNLVIAFPCKEMVLELSRD
                     DLSTVIRNSTSKRISDKRVTYLTEKLTDLAHKSYCAVKKTSPMLEEVRYYAQELLRLS
                     ERRQVVLDEMVSLAQPLPEYEILLSIPGIAETTVTSLIGELGDIRRFQSANQINAFIG
                     IDLRHYESGNFLAKEHITKRGNPYARKILFKCIHNIASASHTNPCHIADFYEKRKRQS
                     QTTSTKPHTIASIHRLIRTMYYLITHNKIYDYNLATSQ"
     gene            complement(41826..42218)
                     /gene="rpsI"
                     /locus_tag="VW191_000484"
     CDS             complement(41826..42218)
                     /gene="rpsI"
                     /locus_tag="VW191_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU9623313.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(42238..42684)
                     /gene="rplM"
                     /locus_tag="VW191_000485"
     CDS             complement(42238..42684)
                     /gene="rplM"
                     /locus_tag="VW191_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU9623314.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT        12812 a         9336 c         7511 g        13212 t
ORIGIN      
        1 ttttccatct ctattattca tattaaaccc tattaataac cttcccttac agttctacgt
       61 aaggaatcat tctcaacttc tccatacctt tcttcagaaa actgataatt tgagtagatt
      121 aaactaccta ataatatcca aaaaatatta taatggatac tcaatatcaa atcgtcaatc
      181 aaagcatgga aactcagaga aactataata atacttaaga caatttgtcc ctttctcaaa
      241 agcttcacta aagtcgcagt cagtaaactt aaaataataa ttaaagctat cagcccaaaa
      301 tgttgaagaa tctgaatata tagattatct acatataaat atgcatccgt acttcgttct
      361 ccttgcgcat tcaaggcgtt tccaacccaa gcaattttct gcccaagaat cccataacca
      421 tataaactta acgaacgatt agataaataa atacgccccc ctaagaattg atctgcttga
      481 tgaaaaaaat gattcaacaa ggcatttgat gtccttaagt aattttttga aatccaataa
      541 cttaaatagg cattaacaaa ataggtaagc gtgaaaaatt ttaataaaag actaaatttt
      601 tcaataacag tagggattag cttcatagcc aaattagcta atagtaacaa actcgaactg
      661 ataaaagtca gtcgtgaatc agtttctaaa tataggtaca ttgaaactaa aaataatata
      721 aaaaggcgct gatacgatac tgtttcttgc ttcaaaaaaa atgatatagc tataatattc
      781 atcatgatcg ttgatggaaa taatgaatat cggaatccaa gatagtgtcg aactctagta
      841 gatgaaaact caatgtaatc aggaataaaa cctatcttac tactcaatat cacaaggaac
      901 aacatagcaa cactaatgat aaaggctact ttgactactt ttgaaaaggg aatatccctc
      961 aagctatata tcagagatag ccctaagact ataaaagaag tatagctcgt tacactacct
     1021 atgataaaat agagcgtaga aacccctagt aaacccaata gagactcaaa gctattactc
     1081 ccagatacaa attccttcaa aattattagc ataattatca aaaagattgc agctttatat
     1141 agcataggag atatatactg aacgtaaaaa cttacatcca atagcgaaaa taggaggaat
     1201 atagctaatg ctgctagtcc taaaatttct ggcagtgaat accttttgat agtgattttc
     1261 atattccctc ctagaaactc tgtccatact tctttccaaa aaatttagcc attaaaaaag
     1321 attttccttt ttcaaacaaa tccactctct ccatgtacat aaatggtacc tctttaggcg
     1381 ttattccttt tttacataac caaacattaa acaacaactc actaactcgc ccgaataaac
     1441 gtgactcaaa tgaagtatag tcggtcaagt ccatctgttc gtacatagta tccagaatag
     1501 aaaaaagcca cggtaaataa tcatctaata gtcctttttt catgataaac atattgaaca
     1561 tataaccgct tctttgtttc atcactttat caaaggatga aagatactct gaactatttt
     1621 gctcaataat tttcctagca agatccagat gttctccgtt aagggtatgg gcataatgtg
     1681 aataaagagt ttcaatataa tactttcgct tctttggaac tattacgtca gatttctcta
     1741 ataatatttc aacattagat cgagacaaaa tgacgtcatc catattgaca ttttcattat
     1801 acccttgaga tttatttgta aaataacgac ggtaatgaac tagacctaag taattataat
     1861 caagattctt ccatgcccaa taaagtcccg tcaactcaca ataatatgga tttagagagg
     1921 atatattatc gccagtatta tcaccgataa aaccaatatc tgatttaccg tctctcccaa
     1981 catgtattgg aaggtaaaga ctattgtctt gaggcatttt aacctcttta tgtgttgcca
     2041 caatgatttt tacatctatc aatctacttc gctccatcct tcatcaatac aactttaact
     2101 gtcttcaata aaatctcaat atctttccaa attgtccaat cgtctatata ggctacatct
     2161 aatttgacaa cttcatcaaa attcttaatc tcacttcgtc cactgacctg ccataagcct
     2221 gttataccag gtttaaaact tagccgacgt ttttgttctg gggtatagtg ttcatactcg
     2281 tccactgttg gtggccgtgt gccaaccaaa ctcatatctc cctttagaac attataaaac
     2341 tgtggtagct cgtccaagct agtcttccgt ataaaacgac caattttcgt gatacgagga
     2401 tcatcgtcca ccttaaacat tccaccctgc atggtatttt gttccatgag ttctcttttt
     2461 ttcgcctcgg catctacaca catagagcga aacttataaa aagtgaactg acgaccattt
     2521 tttcctatac gcgtctgagc aaaaatagca gaacccccat cctttcgaat caaaggaacc
     2581 agtacaatac tgactaaacc acatagtatc agccctacca atgcacccat gatatcaata
     2641 atccgcttag ccattatatg actagtctta taaaatgttg tagaaaaagt cacaacgttt
     2701 aatcctgcca tctcacgaat ttgcttgtta cgtgccaaac tacgatcaaa agcatttaga
     2761 ttaactgtta catcaattcc catcgtttca aactgagaga caagctctcc aatattgtat
     2821 ttttcacttg gaagattgat aaagacttca tcgaccacct catgagtcgc aaagtttact
     2881 atctccccct ctgctactac ctttaaacaa tcatgctgaa aatctggttt atctaagaca
     2941 ctgacggcta ccaacttccc aacaacctca tttgattcta ttaatctatc cagtaccttt
     3001 tcgacatgag aagttgctgt aagtaggaga atcttcttac ttcctttaaa gttgagataa
     3061 gcccgcttcc aataccactt gataaatagg tttagcacat agactaagag agcatgtaat
     3121 gtgaggaagt aaatcatgcc tcgtctggaa atactaaatc gatcctctaa gaaaaaatta
     3181 gaaatactaa tcgctagtgc aaagaatagg atatatttca atgtctggac aagttcaatc
     3241 aaatatctcc ttttaaagaa atcctgtcca taatcactga tataaaagac aaaataatgg
     3301 aggatataaa gtgcaatagc tgttgttgaa acaatctccg tttctctcac agcactaagt
     3361 agataagtca ataaaataac aagaaaactc tggattatgg ccaatgaaga ccttaatatt
     3421 tttccattca tctctatccc ccgacctatt ttttattttt cccgtaatct ccataatttc
     3481 cataagaacc gtatttgtct actgaagtat cgaatttatt caacacaatt cccaaaaacg
     3541 gctttcctgt gtgttccaac tgttcttttg ctttttgaat atcccgacga tttatttcac
     3601 ctgcctccgt cactaaaata gaagcatcgc agtttcgcgt aataatagcc gcatcaatca
     3661 cgacaccaac aggagcagta tctacaatga tgtagtcaaa atatttacgc aaggtttcaa
     3721 gcattgtagt gaaattctta ctttgaagaa gagctgtcgg attcggtgac acagagccag
     3781 cctgaattac aaagagattt tcgatattgg tatcacaaag cccttgtgat aagtctgtag
     3841 ttcctgataa aaattctgtc aggcctgtaa tcttattcct tgctttaaag acacctaaca
     3901 taacagaatt gcgaatatct ccatcaatca gcagcgtttt gtaacctgca cgcgcaaaag
     3961 cccaagcgat attggcggaa gtcgttgatt ttccttctcc tattttcaca gaagtgatag
     4021 aaaatacttt caaaccatct ccacttaact gtaggttcgt gcacaaagcg ttataatatt
     4081 cctctgcctt ttttacagaa tccaattttg cctgtgagat ttctaatgtt ggcataactt
     4141 cctcctattt tattttgtca aaatctggaa cgagccctaa aagtggaatt ttcagtacat
     4201 cttcaacatc ttcaggacgt ttcacacggg tgtcgagcaa ctcaatcaaa agaacagcaa
     4261 ttactgttac gactgctcct ccaagaaaac caaacaagga attgcgtcga acatttggag
     4321 aagagggagt cgtagctggt cgcgcttctt caagtgtcgt tacatcagat actcgcgtta
     4381 cagcgacgat cttttctgca gcaacttctc gtagagaatt agcgatacga ctggcttcct
     4441 ctggctgttt atccttgaca gagattgaga cgatacgagt gtcagctggt acagtcactt
     4501 gaactttgct ggctaacgtt tttgctggca tatccaactt caaatttgtc gctacttttt
     4561 ccaatgcatc ctgcgaaagg ataatctcac ggtagtcttt taccagataa gatcctgcct
     4621 gcaaatcctg atttgtcagc cccggcttgt ctccttgatt gcgattcact acgtaaattc
     4681 gcgtggtact cgtatattct ggcttaacaa taaaagtgct atatgcaaaa gcccccgcac
     4741 ctgtcacaag tgccactatt aaaatcatta gcttgcgttt ccacaaggtt ttaaataatt
     4801 gaaatacatc gatttctatc gtgttttgtt ctttcatcat ttctcctaaa ttagttgatc
     4861 cattacaatt tttcgaggat tgtctataaa aagttcctga gccttcgctt ctccgtattt
     4921 ttgggtaaca aggtcatatg cttctgccat atgaggaggt ctaccgtcta gattgtgcat
     4981 atcacttgca atgatatgaa ccaaatcctg ctctaaaaaa tactgagctc tttttttcat
     5041 gaatttataa cgttcgccaa aaagtttggg tttgaggaca tgtgaactat ttacttgcgt
     5101 gtaacagccc ctatcaatca gttctcgaac gcgtttttca ttattttcaa gagcatcata
     5161 gcgctcaatg tgggcaatga ctggagtaat tcccaacatc aagatcttgc tcaaggcgct
     5221 atgaatatcg cgataaggag tgttcatact gaactctatc aaggcataac gactatcatt
     5281 gagggtcgga atccgctttt tttccagctt atccagaaca tctggtgtgt agtaaatttc
     5341 agccccgtaa gcaatgacca agtcactcgc cacttcctta gctatttccc gaacctgaag
     5401 aaagttttct gctatcttct cttccggagt ttcaaacatg cccttgcgac ggtgagaggt
     5461 agagacaatg gttctcaccc cctgcctgta ggattctgtc aagagagcct tgctttcctc
     5521 tcttgacttg ggaccgtcat ctacatcaaa aacgatatgc gaatggatgt ctatcatttc
     5581 atctaccctc catcacatcc tgtatagctg ctttaactac agctaaacta ctatcatcta
     5641 tttccatcac atagaggtta ctgtctggca ttgcataaga aggaagatcc gtccgacctg
     5701 taccttttaa atcttgagaa tttactttat aattccctcc actttctaac tgagcattga
     5761 ccaaatttat catagtctca agtggcatat ttgtttggat agaatcttgc aagctattaa
     5821 tgatcgtgct ataatttttc agtgcttcgg ttgacgttaa tttttgaagg atagccacaa
     5881 tcaccttttg ttgattgcgc ccgcggtcac gatcgccatc tgctagtgag tagcgctcac
     5941 gaacaaaacc gagagcctgt tctgaatcaa gatgaacatt gcctgcaggg taatactttc
     6001 cattcgtatg ggcagtaaat tcttgatcat tataaacatc aattccaccc aacaaatcaa
     6061 tcaatttcaa aaacgaagtg aagttcaatc gcacatagta attgatatcc actccataga
     6121 gattttctaa ggtgtgaatg gacgaatcaa ctccataaat gcccgcatgg gttaatttat
     6181 ctttttgatt atttccacca tctgcgattg gtacataggc atcacgtggc gttgtggtca
     6241 agaggatttt cttggtatct cgattgacag tcatcaggat attgacatct gatcgcgaca
     6301 ccgaactaat agggccatag gtgtcaattc cactaacata gatattgaaa gactgattct
     6361 tagacgtctt aggagcttct acttttttag tgaatccctt ggtataaatc ttttttatct
     6421 tcgatgcgta gtctggatac tctgactcga tgatattttc aaagacacta tttaggacaa
     6481 tggccttagt ctcccctgca atcaaactct tgtaagctgc caagtaagac gaactctggt
     6541 tgaccgtcaa atcggtattc tgacttgact tgatatcagc tagtagtttt tgaatatttt
     6601 cattatcagt cccagtcggt gctgtcacac tcgtcagttg cgtaacattt tcgatatcac
     6661 tatctgctaa aacagcgaca ctgattgaat attctgagta attagaagtc gcatttaaac
     6721 gattggtcag tccaacaaac tgctgtactg caaagagcga cacagagctg acaaggatag
     6781 agaacagcaa cagaaaaata gtaaattttt cagctttttt atagataacc aagagtagcc
     6841 ctaccaaggc aactagtagg actaacgcag ttaccactag gttaagatat ctaaaagcaa
     6901 ggatattgta cttaaagatt aagaacaata aaaaaccaac taacaataaa taaatagtca
     6961 gcaaaacgat attaacactt cgcttcactt tctgtgaacc tgatttttta aaacgtctac
     7021 tcatgattaa cacctataca ttgaacatca tacgattata tcactttttt acggtaatgt
     7081 ctacaccttt attttcacta tctgcatctt taagtatctt agtagacttc ccgcgaacca
     7141 aaaatatagt aaaatgaaat aagaacatga taaaacgatc aggacagtca aatcgatttc
     7201 taacaacgtt ttagaagcag aggtgtacta ttctagtttc aatctaatta tgaacaagac
     7261 ccagaaaaag tagccttatt tcttaagaat tttaatagtt taaagcacct agcacctgtt
     7321 tagattgacg aaacaagatt cgatacttat ttttatcgag aatatggtcg ctcattaaaa
     7381 ggtcagttaa gaagaggcaa agtatctgga agaagatatc agaggatttc tttggttgca
     7441 ggtctaacaa atggtgagtt aattgctcca atgacttacg aagagacgat gacgagcgac
     7501 ttttttgaag cttggtttca gaaatttctc ttaccaacat taaccacact atcggttatt
     7561 attatggata atgtaagatt ccatagactg gggaagctag aacttttatg cgaagagttt
     7621 gggcataaac ttttaccttc ttcctcccta ctcacctgag tacaatccta ttgagaaaac
     7681 atgggctcat atcaaaaagc acctcaaaaa ggtattacca agttgcaata ctttttacga
     7741 ggctcttttg tcttgttctt gtttcaattg actgtattat cagcgtaata gtctaataga
     7801 tagttaaatg tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct
     7861 gattggccgc aagcagctaa aagcaaagca gatgcaaaag tcagacctgc actaaggaca
     7921 cgcttcttta tgtttatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat
     7981 ttcgagaatg aaagtttata aaccttggtt ataaaaaaac aaaggatttc tgtcttttat
     8041 acagtcctcc cttgttttta tatgatttca attttaaatt tttctgcaaa aaatatttat
     8101 agtaattcca cacagaaagc atcccatgga gccaagacct gtttttcaag tacttcttta
     8161 gccgcagtgt tttcaatcaa gacagatttg acttttcctt ctactgtcaa gtcttgctct
     8221 tcattggaca agttagccac aactaggaag cgacggtcgc catccttacg tatataagca
     8281 aagaccttat cagccgtatc aagcaattca aagtcagctc gaactagcca gctattctcc
     8341 ttgcgaattt ggaccagttt ctgataggta tagaaaatag aatctggatt tgccagcgct
     8401 tcttggacac tgatcatctc gtaatttgga ttaaccgcca accaaggttg acctgttgag
     8461 aaaccagcgt ttttgctctc gtcccattgc ataggggtac gggcattgtc acgtccaata
     8521 acacggatac tgtccatgat ttcttcaatc ggaacacctt tttcaagagc ctcacgcgca
     8581 tagttgagag attcaatatc ttctacttga tccagtgttt caaacggata gttggtcatc
     8641 ccaatctcct caccttggta gatataagga gttcctctca tgagatgaag taagattgca
     8701 aaggctttgg cagatttttc gcggtattct tggtcatttc cccagattga gacaatacga
     8761 gggaggtcat ggttgttcca gaagagggaa ttccagccgt cctcaactcc taactctgtc
     8821 tgccatttgt tgaagatttc ttttaactta gcgatattca gctctttttg atagtgccat
     8881 ttaggctgac cttcctgata ctgaagaccg atatgttcaa actggaagac catagacaat
     8941 tcttgcccct ttggatcaga gtagagcttg gcaatctctg gtgttgctcc ccaagtctcc
     9001 cctactgtca agagatcctt agctccaaag gtcgcctgat tcatttcctt gagataggga
     9061 tggagcatag gaccattatt gactaccttc tcgtcaggaa ttttgccaat catgtcaata
     9121 acatccatac ggaaaccacc aataccttta tcaatccaga agttcatcat ctcataaatt
     9181 ttctggcgaa gtttttcatt ttcccagttg agatccggct gtttcttgct gaaaaagtgg
     9241 agatagtatt gacctgactt ttcatcgtat tcccaagcag acccactaaa gatagaatct
     9301 aaatcgttag gctcatcccg ccagatatag tagtctcgct cagggctatt aggattttca
     9361 caggcctcta caaaccaggc atgctcatcc gaggtatgat tgaccaccaa gtccatgatg
     9421 atacggatat cacgcttctt agcttctgca atcagctgat ccatgtcctc catggttcca
     9481 aaaatagccg caatcgcttg ataatcagca atatcatagc cattatcatc catagggctg
     9541 tcataaacgg gagaaagcca aattgctgtg attcctagct tggctagata gtccaactta
     9601 ctggtaattc ctggcaaatc accaattcca tcttcattac tatccataaa actctttgga
     9661 tagacttgat agactacggc attatgccac catttttctt gcatcttctt acctcattat
     9721 tttaataatc tatagtatat gatagcactt tttaaaattt tgtgcaagcg tttgcttaat
     9781 ctttttaatt tcttttttag ctccgtagtt tcctaacttc caatttttta ttataataga
     9841 gtccagaggt aaaaaaatga aaaaacaagc ttttagttct gaacaatatt taaatctaca
     9901 acgcgaccac atcttagagc gcattaacca atttgacggc aagctttacc tagagtttgg
     9961 tggcaaaatg ttagaagatt tccacgctgc tcgtgtcctt cctggttatg aacctgacaa
    10021 caaaatcaag ctcttgcaag aattgaaaga gcaagttgag gttgtgattg ccattaatgc
    10081 tagcaacatt gaacattcca aagcacgtgg cgacttaggc atttcttatg accaagaagt
    10141 tcttcgtttg attgacaaat tcaatgaatt agggattttt gttggttccg ttgtcattac
    10201 acaatacgct ggccaactcg ctgcagatgc cttccgcaac caacttgaga aaaacggaat
    10261 tgattcttat cttcattatc caatcaaagg atatccgacg gatatggatc acatcatttc
    10321 cccagaaggc atgggcaaaa acgactacat caaaaccagt cgcaacttga ttgtcgtaac
    10381 cgctcctgga cccggttctg gaaaattggc aacgtgtctg tccaatatgt accacgacca
    10441 aatcaatggc atcaagtctg gctacgctaa atttgaaacc ttccctgttt ggaatcttcc
    10501 ccttcatcat ccagttaact tggcttatga agctgccaca gctgaccttg atgatgtcaa
    10561 catgattgac cccttccatc ttcaaaccta tggagaaacc actgtcaact acaaccgtga
    10621 tatcgaaatc ttcccagtgc tcaagcgcat gttggaacgc attctaggag agtctccata
    10681 tgcttctccg acagatatgg gtgtcaacat ggttggtttc gctattactg atgacgaagc
    10741 ggctatcgaa gcttctaaac aagaaatcat ccgtcgctac tatcagactg ttcttgattt
    10801 caaagccgaa aaagttggcg aagctgctgt caagaaaatt gagttgctca tgaacgacct
    10861 cggcatcaca cctgcagacc gtaaggttgc tgttactgca cgtcaaaaag cagaagaaac
    10921 tggcggacca gccctagccc ttgaattgcc aactggggaa attgtcacag gtaagaactc
    10981 agaactcttt ggtcctacag ccgctgcctt gatcaacgcc atcaagaaat cagctgacat
    11041 cgctaaagaa gtaaaactaa tcgaacctga agttgttaag ccaatccaag gtcttaaaat
    11101 tgatcatctg ggtagccgca atccacgcct tcattcaaat gaaattctga ttgcccttgc
    11161 tatcacagct acagaaaatc ctgatgctgc ccgcgctatg gaagagcttg gcaacctcaa
    11221 aggaagcgag gcccactcaa ccatcatctt gactgatgag gacaagaatg tccttcgtaa
    11281 actgggtatc aacgtaacct tcgacccata ctaccaatac gaccgcttgt atcgtaagta
    11341 aagatttgaa cctctccact ctcggagagg ttttctctct gtctcaggag ccggctttat
    11401 gttataatga aagaagaaaa ctgaaaggat ttaccatgtc aaaagaagtt attgttgaaa
    11461 gttttgaact tgaccacact attgtcaaag caccttatgt tcgcttgatt ggggaagaaa
    11521 cagggccaaa gggagatgtc atctccaatt atgatattcg cttggtgcaa cccaatgaag
    11581 actctatccc tactgctggc cttcacacta tcgagcacct cttggccaaa ctcatccgta
    11641 cccgtatcga cggtatgatt gactgttcac catttgggtg ccgtacaggc ttccacatga
    11701 ttatgtgggg acgtcatact agcgctgaga ttgcagcagt tatcaaggat tcgcttaagg
    11761 aaatcgctga aactactact tgggaagacg tccctggaac aaccatcgaa tcttgcggaa
    11821 actacaagga tcacagcctc ttttctgcta aagaatgggc gaaactcatt ctagaacaag
    11881 ggatttcaga tgatgccttt gaacgtcatg tgatttaaac acaaaaaagg aaccagtttt
    11941 tttcagctgg ctccttcttt ttttattctc aaaattttgt cttatactca atgaaaatca
    12001 aagagcaaac taggaaacta gccgcaggct gtacttgagt acggcaaggc gacgttgacg
    12061 tggtttgaat ttgattttcg aagagtatta ggctttttca cgaataacta aaccaccatc
    12121 ttccatatct gcttccagat gtttggcatc taggtgatcc aagtggaagt ctgtcacctt
    12181 atcacgaatt tcttgttcaa ccacgcgacg gagagggcga acacccatga cttcgtcata
    12241 gccttcttct gtgatatagt ccttagccgc ttgactgact accaaatcaa tgcctttctt
    12301 agccaaggtt tggttgactt cagccaacat caaatccaca atcttagaaa gatcttcctt
    12361 ggtcaagtgt gagaactcga taactgcgtt aaagcggttg aggaactctg gtcggaagaa
    12421 tggtttcaaa cgatccatca attctggttt atccgcatct tctgtcaagt tggcttcata
    12481 accaaatcca gcatttgagg tcgcgataat gacagtgttt ttaaagttaa cggtatttcc
    12541 ttgaccatcc gtcaaacgac catcatctag gacttgaagg agaagggtaa tgacttgagg
    12601 atcagccttt tcaatttcat ccaagagaat gatagagtaa ggattgcgac gcacacgttc
    12661 tgtcaaggta ttgctattgt catcataacc cacatatcct gctgttgtac caattagctt
    12721 agaaacagct gtgcggtcac tgtattcaga catatccaaa cggatgatcg catccttagt
    12781 tccaaacata tcgagtgcca attgcttagc aagttctgtc ttaccaaccc cagttggtcc
    12841 tacaaagagg aagctaccaa ttgggcgatt accttcatca aaaccagcac ggttacgacg
    12901 gatagcacga gctaccgctt ctaccgcctt gtcttggcca attaccttat cttgcagacg
    12961 atgagccata tctttcaaac gctcgatgtc tgacgctccc atttgtgaca ctggaatacc
    13021 cgtcattcgt tctacagatt cagccacatc gttgacactt gcagtcactt tcatatcttc
    13081 tgtatggttt tcgattttct tctccaattc tgcaatgcgt gttttatagt tgagagctgc
    13141 ttcaaaatct tctgcctcaa ctgctttttc ttgcttgtct ttctccgctt caatttcacg
    13201 ttcaacagca tgcacatctg ttacaggatg ctgagctgcc aaatgagctg ccgttacatc
    13261 gacaaggtcg atagccttat cgggcaaact acgttgagga atatactgaa cagaataatc
    13321 cactgctgct ttcaaaacct cgtctggcaa gatgacattg tggtgttgtt gataaaggtc
    13381 acgaattcct tgaaggattt taaaggtatc ctctgctgaa ggagcattta ctttcacttc
    13441 gttgaaacga cgagcaagag ctgcattctt caagatggtg ttacggtatt cgtcttgagt
    13501 tgttgcccca atcactgtca attcaccacg agagagggct ggcttgagaa tgtctgcaag
    13561 tcccttagat ccactatctc caccagtgct accagcaccg agaatttggt gaatttcatc
    13621 aaagaagagg ataatattcc ctgcttcttt cacttcattg actaaatttt gaacgttttc
    13681 ttcaaaacta ccacggtatt gagtcccagc ctcaagacct gaaatatcaa tggaaataat
    13741 ttccttattc ttaatagcag ctggaacatc gccgttcaca attgcttgag caagaccttc
    13801 gacaacagct gtcttaccaa cacctgcatc tccgaccaga acaggattgt tcttggtacg
    13861 acgtgagagg atctcagatg tttcttgaat ttccttgttt cgtccgataa caggatccag
    13921 cttgccctcg cgagcttctg ctgtcaagtt tcgccctagt ttagcaagga caccatcttg
    13981 tttcatacct gaagcctgtt gtggcatttt gccatcaact tctacatttc ctggtaattg
    14041 accagttgca cgatagtgag caaattcctc aggtgtgact tcacgtccat taatcaagta
    14101 acgacgattt tcagaactgt atccgcgcat accacccatc aattggttaa ataaatcatc
    14161 catgttgtta aaattattaa agttgttgtt catattcttt acctcttttt gttatttatt
    14221 attactgata ttgactatct ttgacctttg ttttaaaaaa tttagactag ctaaatcgct
    14281 actctacgta ctatttctgg tttttctttt tcaagcagga gtagactatc tttacttcta
    14341 aagatttcta gatgaaacat agacccacct ctttcttttt tactttaaat aagcgttcta
    14401 gtaaggcttt catttacctt acgttcataa tatactacat tagtcagaaa aggtcaagga
    14461 ttttgacttt gactgaccaa tattttttat actcttcaaa aacctcttca aaccacgtca
    14521 gctctatctg caacctcaaa accgtgtttt gagcagccta aagctagctt cctagtttgt
    14581 tcttgatttt cattgagtat aagtgttcac gtaaggtttt catttacctt acgttcataa
    14641 tatactacat tagtcagaaa aggtcaagga ttttgacttt aattgaccaa tattttttaa
    14701 aaagcaaaaa caccctgaaa aatcagggtg ccgttcttac atcaaataca aaattgctag
    14761 cgtcaggaga cttgctagaa gtcccactcc ccaaaagaag aagtcaacct tccactcgct
    14821 ccaaggattt cctttaccag acaatcctcc aagctcaaaa tggtgatgca caggcgtcat
    14881 acggaaaata cgtttgccac cagtcatttt gaaataactt acttgcatca taaccgaagt
    14941 tgtttcaaaa acataaacaa ttccgataat caagagagtc cattcttggt ggagggccat
    15001 agagatagct gccaacattc caccgagagc caaacttccc acatccccca taaagacctt
    15061 agcaggctta tggttaaaga caaagaaacc gagcaaacca ccaatcatgg caagaatcac
    15121 tagaaggata tccatctgac cttgcacata ggcaataacg ccataggcag acaaactaat
    15181 cacaacggaa atactagcca gaccgtcaat accatctgtc aagttcactg cgtttgaaaa
    15241 accaactagc cagaaaagag cgaagacaat atagaaaatc cctagatgta cttgataacc
    15301 aaagacagaa agcatatcgc caccacgctc atagaaaagg tagaaaatga cgccacctag
    15361 aagctgaaga gccaatttct gtttgggatt aaggccctca ttgattttac ggaagacctt
    15421 gaggaagtca tctaaaaatc cgattaaacc ataaagaacc aagataaata aaatcatacc
    15481 aacattattg ctgagttgat tactaaatag ggcgaaaaag aaagcaacta aaacagaagc
    15541 aattaagaaa accaaacctc ccattgtagg agtcccagct tttgcctggt gctgtttgac
    15601 atcctcatgc atctgctggc ctgtaatttg cgcctttcta taaaattgga taaaggctgg
    15661 aattcctacc aaagttagta aaaatgtcac aattccagca ctgatagaaa taaacatatt
    15721 agtctcctaa agttaatgta atttttttaa tgtttttgat agctgtatta gtccgaacat
    15781 cttgcttctg aacgacggaa cctgaacctt caaattccaa ctcaatatcc aaccatttag
    15841 caaaggtttc agcagtctct tttttccagc catacatgtc tggaatttct tctaccttat
    15901 ccgataaaag gagaacttgt tggtttggtg caagattggt cccttcttct acagaagtct
    15961 ctttaatctt tgttccagta cctacaacga ttggttgcac aatatttcgg cgtaaggctt
    16021 ccgccaactc accaggtgaa atatccttga tgctaggcat tgcataagaa gattctgtcg
    16081 taactttatc taaatttttg gctggagatt gaagattgag agattctttc atagctgaag
    16141 cccgctccaa gattggggtg gcaaattctc ccaactggat acctgaataa tgctcaggct
    16201 gttgaaccgt tacatacaag ataaaatcag gattttcagc aggattcata gtcacaactg
    16261 agaaaatata attggtagaa ccaaccaagt atcctccatt tttctcatca gcgatttgag
    16321 ccgtaccgga tttaactgct acattttgtc caggaactgt tataattggc tttcctgtgt
    16381 agtgattata catagttcca tatagagggt ccgtcccaac taagatcatg tgatttcgag
    16441 ttgtgcttgc tgcctctttg gaaacaggat ttcctactat ttctttttgt gacttacgta
    16501 cagactgatt gttagtatca taaatagcac ttataaattt tggctccagc ataactccat
    16561 cattagcaat agctgtaaag gcacgaagca tttgtgtttg tgtcactgaa attccttgcc
    16621 caaatgagct ttgagcaata ctaacaatat tatcagctgg aagttgacca gcgtattcat
    16681 ctgtcaagcc aaagcgagtt ggaaccccaa atttaaagcg ttttagataa tccaaccaag
    16741 tagcatctcc cattttttgt tcaagtagac tcattccaac attactggag tgagcgaaac
    16801 cttgtaagaa agtcatcatc ccaccagtag tcaaaccttc attaacatcc caatctcgag
    16861 tcgtcgcatc cgctattttg aattcactgc tattgaagta ttctccactt gggaaggtat
    16921 tattatcaat agaagaagct aacgtcataa ccttcatggc tgatcctggt tcatagttac
    16981 tttgataaag aatatcacgc caaacaaagt cctcagtgat tccttcttta gtatctgcat
    17041 taaaggtagg tcgttgggtg gtagcgagga tttcaccggt ctttgcactg accaaggtcg
    17101 cggtcatata cttacctttt actttttcta gaaaggcatc catctgagtt tccatgaaag
    17161 attgtagcgg actagacaat gttgtataaa catccttgcc atccacagtt tgttgcgata
    17221 ccagttctgt acctggtacg atatttccta cacggtcttt ttcataggta ataataccgt
    17281 ctgtcccagc aagaatggta tttaaagaac tctccaaacc agaagtccct aacaaactct
    17341 tactaccatc ctcattttca tgaagttggg ctaagccaat aaagctagaa gcaaattgtc
    17401 cgtttgggta actacgattg ggactggttg taaaatcaat ccccttgacc tctgcagttt
    17461 ccaactcttt tttgatagac atcatattgg cataggtaat cccattgccc tttgctccaa
    17521 aggaaacttg cttgagatta ggttgcgaga gttgctctct tacataagat tcttccatgt
    17581 ccagatactt atgaaagacc tctgcaacct tgttaaattg tgttttttct acgtaaagaa
    17641 tcttacccgt tgctgactta tagttctcat caatgaccgc atagacatta taggatgttg
    17701 catcctcagc aatcgggact ccatttcggt cataaatagt cccacgtttg gcaggaactg
    17761 tacgggtggt ttgatgaacc ttcttagctt ccttcgctaa atctgttcca aagcgagtgc
    17821 ctgtcccaat aatgaccgca aaattgacta aaaaaatggc aaaaacaaag acagataata
    17881 aactcagact ttttccaact ctgcgtctgt tttcagccgg cgatttccga tttttggtcg
    17941 cataacggat tactcttttt gtccacttca tatcttactc cgctattcta atattttcat
    18001 tgtttaattg caaatcgtgt gaattggcaa tttctttcaa acgttctgca cgtaatagtt
    18061 cattgacctc ttgcttggca tcgtccaatt cggttttctt ttcctctatc tgcgcattga
    18121 tttttgtcaa atcattctgc acttgcaaga gcttggtctg cataaaaata atactaatgg
    18181 ctacaataag agtggttaca gcaatggaaa agtaaaaagc tttttccaca cgcgaaaacc
    18241 gtttaagttg catctgtagt atttgacctg ttttttccat tttttctgcc atctttttcc
    18301 ctcttacttg tgaatttttc tggccacgcg caacttggct gagtgcgagc ggttattggc
    18361 ttctaactct tccgcacttg gcaagattgg cttacgagac accaattcca tcttgggctt
    18421 gagatcatct gggatgaaag gcaagccttt tggaacttca actgttgaag cttccttgaa
    18481 caattgcttg gtcaagcggt cttctaagga atgaaaggta atcactgaaa ttctaccatc
    18541 cagagccaac atatccatag cctgctggat ggactcatct gccgccccca gttcatcatt
    18601 gacttcaatt cgaatagcct ggaaaatctg cttagcagga tgccccttct tcttgagttc
    18661 cttggcaggt ttgaccaact tgataatctc tgctaactca gtcgttgtct caatcggctt
    18721 cacttcacgc gcttgctcaa tcttacgcgc aatctgttta gagaatttgt cctctccata
    18781 cttgaagaaa atacgaacca agtcatgata gtcataattg ttcaccactt cataggctgt
    18841 cagactagca tcctgattca tccgcatgtc cagtggcgca tcctttttat aagaaaaacc
    18901 acgctcacgc tggtctaatt gaggactaga cactcccaag tcataacaaa ttccatcaat
    18961 ttcctgaaca ccagcttcgc gcaaacgtgc ctgtaaatga cggaagttgt ccttgataaa
    19021 ggtcaccatt cccttctcaa tgtaaggtgc caagcgtttt tgcgcattgt caatggcatt
    19081 ctgatcctgg tcaaaggcat agagatggcc tttttcactt aatttactta ataaatactc
    19141 gctatggcct gctccaccca aagtcgcatc aacgtagata ccgtcaggtt ttacgtcaag
    19201 catatcaatc gtttcgtgga gtaagaccgt tacatgatga aattcttttg tcatatctta
    19261 tctattttac cacaaatcca accagcttgc acttgtaaga caaataggac aaggagacta
    19321 ctatttcttc aaaaaatatg tcaaaaatac ttgacacttt atgtttaaaa gactataata
    19381 aagtcaaata tatacgacat aaatcagaaa ggagaacaga tgaatcgtgt gaaagaattt
    19441 cgcaaggaat tgggtatttc ccagctcgaa ctcgccaagg atatcggtgt ctcgagacag
    19501 actatcaata tgattgaaaa cgacaagtac aatccaacct tggaactctg tctcaatctc
    19561 gcccgcagcc tccaaactga cctcaacagt ctcttttggg aggaagattt ttaaaaaagg
    19621 agcaaactca tgaaaaaaga aaccttcact gaaaaactga tcaaacgcac atacggtatt
    19681 tctggtcccc ttgacgaata caaacggcgt gaggccgatc gtattgggaa ccaagtcttt
    19741 atcatcctct tttatctgat gattttcgga aatcttattc cactccttct ggcctataaa
    19801 taccctcaag aagtggctct aatctatcct cctctgattt tagtgattgc cctcatcgct
    19861 gctggctatg tcacctacca aatgaaaaaa acagggatta cagctattga tccagacatg
    19921 ctgagcgaga aagaaagtaa gcaactacat tacccaggtc tgaaagcagg tttgttcttt
    19981 ggtctatgga tgttttttat aactcctctt ctcaggatac tcataggtga aggtcaggac
    20041 tattttcatt ctcttctcac tataagaaat ggtgtatcaa gcattctcgg ttctattttc
    20101 ttcggagcga gcatacagtt cctcatctcc cgtcgcattg caaaaactaa gaaaaatcaa
    20161 gatgaggatt aggaggtgcc ctatgaaaaa agaaaagaga cagttacgtt atcctggtct
    20221 gaaagcaggt tcgatttatg gaacagtgat attttttata attccactca ttgataccct
    20281 aacaagtgaa aatccaaatt ttatcagttc tcttctaaat acaaaacata tttttaaaac
    20341 tatactggga gctttctttt tcggagtaat gatacatatt gtcgactcac ttcgtattgc
    20401 aagagctaaa aaagaccagg attaggaggt gccttatgaa atcactagct agactactga
    20461 tcattcatgt ttttatcagt attttccttt tcttcgccct tatttcagga gctgtttctc
    20521 atacagtttt actactccta ctcctctttc ttcctgcgct caataaagga cttgagaaaa
    20581 tacaatcaaa acggatacct gtcctcaacg cagccctctt ctttctcctc atatcctttc
    20641 cacaactttt aaccaaccct gtccaatgga aattttcaat attcctagtc gtaaccatca
    20701 tttcaagttt ggcctacttc tataactttt atcaagtagt taaagaagta gatcaaaaac
    20761 agttgattta ggagcttgct cccataattt ctcatttcat aggcaaataa tcccacctag
    20821 cttgttctag gtgggattat tttatttctt aatagagatt gcaagtcatt ttcctttatt
    20881 ttagaaagtc gactctttcc aattcacact acaatccaaa acttgttgcc tttcttcttg
    20941 attgcggcct tcaatctggt caatcaaaat ctttgtagcc tgttgtcctt cctcaaagga
    21001 gggctgaacc agcgtcgaaa cacttggaga agaaaagcaa gtccactccg tattgtcaaa
    21061 accaatcaac ccaacttgcg gcaagttata attcaactct ttgataacgg taaagactag
    21121 aggtagggcc caacagttag ggataaatac cagagttttt tcatcgggat cgatttcttt
    21181 ttgtaaaaat tccttaattt gttccaaatt cgtatgctta tcttcaatgg ttagactggc
    21241 gtgacgcata ttagcatctg ttaaagcatc cacaaaacca cttgcccgct caatccgagt
    21301 actcaaacga ctcgtatccg ctgtaatcaa gagaaaatgc tcataacctt tttcgataca
    21361 ggactgggtc atgtcataaa cggcatcata gttattggtt ttaacccagc tagtccggtg
    21421 ttcatagagc tgactatcaa aaaagaccat tttcttcttt ttctcatcga tgatacgaga
    21481 atattttcgg aaattagagg tcggctgaat aataaagccg tctactccca agagaagcat
    21541 gctttcaata taccggtcct cactctcttg gctgtaatta ctatttccta tcattacctg
    21601 gtagccattc tggctggcga tatcctcaat tcccttaaca atttggtttg agaaactgtt
    21661 ggtaatatca ccgatcaaaa caccgattaa ttttgttcgt ttggagttta agctacgcgc
    21721 aacaatgctc ggtttgtaat ttgtttcatg aataactttt tcaatctttt cacgtgtctc
    21781 ttgggacatt ttttcatatt tcccgtttag gtaaaatgac acggttgttt tcgaggtctg
    21841 agccatttcc gcaatgtctt ttatggtcag tttcttctcc aatctaaaac acctcctact
    21901 aatacttatt ataacatatt cactattcaa aatgcctatt tctagcactg caagcgaatc
    21961 attctctcat ttattttatc atatactaga cattttccac gcatttttgt tccgtcaacg
    22021 aggcacaatt tttcacctac acaaatatcc tcgcttaata acagaatcag cttgtctgtt
    22081 tcgtcatttt tcacttcaaa agccagagca ttttccactt cacgatcatc tgtctggtag
    22141 acagaatgac ggatgatttc aaaactctca tgcgcaatga tggtataatc cagcttctga
    22201 ttctcaaaga attggcgctt ggtgagtttg ctacttcttt caagctcatt gtatttagga
    22261 gaaatgatgg tatcttccaa atcaaaatca acttcactcc atagtctcaa ctgattgatt
    22321 ttcccatctt gataggtcac atccttgtca aggataaact gagtcaacgc ctcatgctga
    22381 ccttgacacc tgatgtcatc caccaagagc cagacatcct ctaccaacat gaggattttt
    22441 cttctgtgaa gataaggcaa atcaggttct gctgaccaat aagccccttc aatataatgc
    22501 attccctccc tttctttatg gtgacaaaac agggagtgag gatagtattc atattcccag
    22561 gatcctgtga ttctttccgg agctttccca tctacaatgc aggtcgaatg actccaagca
    22621 ctctttaaga gataacgttc atatatctcc cgataagaat aacgcccagc atctatgaaa
    22681 ataggttggc cttgatactg taagcaaaaa ctattctcgt cactatggct atgggcactt
    22741 cctagcggac catttttgaa aaatagataa cgatgttcat ccttaatgca gacatgtcca
    22801 gagtcttcaa agatcatgga cttaggctgc caagctctcc tttcaaattc ctgcagtcgc
    22861 ttgacctttt ctcgccccag gaacaagatg ctaagcaaat caactttaac atccagaccg
    22921 ttaagaaggt cttccttgtt caaaaccaca gcagacaggc tcaacatttc tgtcgtttct
    22981 gtagaatcgc tatcaccaaa agccaaagtc cgtccatcta agcctgtcat catttgaata
    23041 taggtcgcca tcttttccag caactcttgg taactatctt gcaagtctgg aagcaagaga
    23101 cacaaatcca gcaaggcttt ataaacctct acatgataga gaatcgactg ttcaaactgg
    23161 cttccatctc ctaaaatctg tgtctcaatt tgctgtttca actcctctga agcaaaatgg
    23221 taagcttctt ctagatccat cttatctgaa aagaaatgat agatagcaag catcggaatt
    23281 gtttgtaaaa tcccccagtt actaagggtg tacttggcgc gatagtagct tttcataaag
    23341 tcaatctgct tttctagact gaccaaaatt ttctctagtt ctttctcctc tagcaagtca
    23401 aatttcaaga ggagcaagag tagtttcaac caagtaaagg aacgaatacc cgtatccaag
    23461 attctagtca tcaaggattg aggagaaaat tctctcacct gctcaatcca atcaaataga
    23521 aagaacttgc acttttgaat atagtcctta tctccttcta ccagataccc tatcataaac
    23581 tgcaagagat attcttgtcg attgagcata taagaccatt ctggatcatc ttcaaatact
    23641 tgatcccata ccatcggctg gatttgatgg atttttgaac aaggctccat atcccaagga
    23701 ctatcaaaca taaaacgatt gtccatcaag cgttcaaggg aactcttgac tttctcatag
    23761 tcttttgaac agtgggacaa gatataatca cgacattggt ttccatcgac tctttcaaaa
    23821 aattgtcttc tttcttcttt cattatctat taccagaaaa agaactactt aaaaagcagt
    23881 tcttttgtct ttcccattac actttccttt tctacataga tgaccacacc ttttgcaatc
    23941 tgcaaggaga ccaagtcatc tttgatagaa atgatttttc catgaattcc agacaataac
    24001 aacacttcat caccagatgt taaagaagct aaatactctt gtcgttgctc catctgtttg
    24061 cgaagcaact tttgctgacg aatagaatga aagcttgaca gtaaaagggg actcactgcc
    24121 aagacaatca ctattccata aaacaatgtt gtatccatta agctataatc ttaagccagc
    24181 ttccgataat tccgatgata actgttaaaa taacgagttt atatgttgtc catttctttt
    24241 ctttgatcaa gtagtaaact aaaagtgtaa atagggctgg tagaagagct ggagcaacct
    24301 tatcaagcat tccctgaata cttacgatac tttgtttagc gtctgcttta acttcccctg
    24361 cagcaaaggt aatcggcacc ataatcttaa cagatgtcgc tgccaaacca gcaattacgg
    24421 ttacaccgat aatattggca atacgagaaa tcgttgccat ctgttcgctt agtttatcaa
    24481 tcacagttgt tcctagtttg tatccataca gaccagttga caatttaatc gctgttaaaa
    24541 tcgtattcat cgcaaggaag aacaagattg gaccgacaac caagccttct tgagcaaacg
    24601 aagctgcgat ggttgagaac aatggagcta aacagaattg agaaagagaa tccccaatac
    24661 ctgccaatgg tcccatcaag gccatcttga tgctacgtgt ttcttttgtt ggtcggccat
    24721 tttccaacat tacaagatgc aagctggtaa taaaaggcag gaagtgtggg ttggtattat
    24781 agaattcaca gttttcttcc aaggcttggt agaaaccttc ctgatcctct ccatagtgtt
    24841 ttttcaaagc aggatacatc acattggcat atcccaaccc ttgatagtta ctatagttaa
    24901 atccattttg acaaaagaat gcccgcaaag acgttttaag ataatcacgt tttgttaatt
    24961 tgttagatcc agtcatcgtg tgcttcctcc tctaccacat gatccgctgt ttttggcttg
    25021 ttataaaatt caatcaaagc aaagatagta cctacaattg caataccaat tgttgggatg
    25081 tttagataag ctgcacaaac atatcccaac aagacaaagg gaatcaactc tttcttagcc
    25141 atcactgaca agatcatcgc aaaaccgata gctgggagca ttttaccagc aactgtcaaa
    25201 cctgtaagca atactggtgg aatgtagtct acgagtttca acaaggtatc cattgaaagg
    25261 gcacccaagc aacccaaggt aaatccaata aaggcaaaca accaaattgt tgcatttaga
    25321 gtgaacttga atttcttcaa attatggttt ttcaagtgct tcatcgccgt ttcaggcgca
    25381 ccagcacgta cagtgtaggc gaaagtttgt aagaattgaa tcgccacagc aatcggagta
    25441 gagagggcaa gagccgcttc tggactgact ttaccagcac tagtgatagc catcaaggta
    25501 ccaaagatac caggtccgat tgggtttggt ggaacagtac ctccagcacc aacaccgaat
    25561 cccatataag ccaattcacc aatagctccc attgcaagag cagtaggcaa atcacctaga
    25621 ataattccga caccaaatga cagaacaata catctattgg tgtaaattcc taacagcatt
    25681 ccactaaaac agaaagctgt ccataatcca attaaaatcg cttgaaatac attaatcgac
    25741 ataacgatct cctcttttaa atatagtcca taatgttgac ttgaacagct ccatcatttc
    25801 ctgttggagt tgttttcgta ttaaatgtta cttgatgagt ttggctcaat tccttgaggg
    25861 ccgccttgtc ctcttcaccc aggaagatgg agcgtgtcac ttgctcttta ccagtagcat
    25921 tgtgaatgtt cccaatattg atttctttga tagggacacc accttctacc aaggttaaag
    25981 cgtccttcac atcctttaca acgataaaga tcgtttgagc aggattagcc ttgtgaatga
    26041 tatcaatcac cttttgcaaa gggaagaaac gcatggcaac tgagtctggc acaactgttt
    26101 tcatcagagt ttgttgcatc ttgtctgtgc ttacttcgtc attggcaaca atgaccgtat
    26161 tacaacctag gtattttacc caaagttgtc cttgcccatg aatcaaccgt tcatcgatac
    26221 gggtcataat aatatttggc attgtcatat ttctcctcct accaatatag gttccagtct
    26281 ttgtagaaac ggataagggc ttctaggtaa tagtagtcac cccagatatt gccttcatcc
    26341 actcctttac ctgaatgcca tgagtacaca ccgtggagga gacttgtccc accaggggta
    26401 aattgatcat ttgcataatg ttcgatcaag gaacgaagca tggcatgcat agcatgttta
    26461 taaatatctt tgtcagcatc cacctctggg agatgtttta gcatttcatg aatcccacag
    26521 acggcgatag ctgttgctga agaatctcgt gattgagcac taccatcatt aaaaatcaaa
    26581 tcccaatagg acacatgatc ttttggcaga cgattcaaga aataattggt cacaccctta
    26641 aacaagtcaa agcaggactc gtcttttaag tgacgataag tcaaaggaat accatagact
    26701 ccccatgatt gaccacgtgc ccagcatgaa tcatcactat acccttgtct cgttacacct
    26761 ttaaagggtt gacctgtctc aggatcaaaa tagaaggtgt ggaaggacga agcgtcatca
    26821 cggattacat tattagctga agcatagaaa tgactttctg caatatcgta gtatttttga
    26881 tcgcctgttt cttgataagc aaagaataag agttggatat tgagcaagca gtcgataatc
    26941 aaacggtaat gctctttctt gcccaagtct ccccaagctt gaataaaacc acctttttct
    27001 tgatagcgtt caatcaactt atctgcagct ttcaaggttg cttctctagc ctctccatct
    27061 ccatttatct tatattcagc catacaagac ggtgtgtaca agaagccgag atcatggtga
    27121 tccaattcta ctctcttatt gacacgatcc aggaaagaaa gaacattttt atgagcgatg
    27181 tttttaaatg catcctgttg actgtattca taagccaacc acagttctcc tgtccagaaa
    27241 ccattggtcc attccgtgtt atccatgatt ggatagacat tatcaaaggt agctggcgtc
    27301 gggaaatctt ccttgaaata gtcaaggttg agttctaact gccgaataac cttatcgatt
    27361 gcctggccga cttcttcttt cgtcagaagt ggtacttcta agaagcgctc aggcgatttt
    27421 attttttcaa tcgtaacctt ttttatcatt cttttaccct ttaaataccc gattcgaaat
    27481 cttcttcctc ttcctctgca tccgttgaaa acaattcttt accatttacg actccgccct
    27541 gggccgccac tactgattta ttaacaacct catcaaagct atgagccatt ctagcaaaga
    27601 ctgcttccat taacatggct aagttcaaac ccgaaagaac attcattgtt ttggctggat
    27661 tttctcccat tatggtagaa gaaaccttga atggcgatcc tcccaagaga tcacttagga
    27721 ttaaaacttc ttcttcattt gaaattgcaa gtaagatttt ttgcttgagt tcatctgctg
    27781 gcattccttc cacaaagtca attgcctcca cattttcttg attgcctgca atcaattgta
    27841 aagaactata aatccctgta gcaaaatgtc catgccctac aagtacaatt ttcatctttt
    27901 ctcctttctt gtttaggttt accggtttac tttatattcc ccattgtaat cctttatttt
    27961 tatcttgtca atagtttttt taaaaaaata ttagcgcttt cattatcttt ttaatcattt
    28021 taatagacag aaatttcaaa atattccatt tttaaggaaa ataaatcttt ttataaataa
    28081 aaatatttat ttattgacaa aaccggttta ctagaatata atttggttat agagaagtct
    28141 taacaaattt gaaaggaagt atcaaatgac aaatacatca ttctcaattg agcagttttc
    28201 tttaaaagga aaaattgctc tcatcaccgg cgcttcttat ggaattggat ttgctattgc
    28261 caaatcctac gctgaagccg gcgctactat tgtctttaac gatatcaatc aagatctggt
    28321 caataaagga attgaagctt atcgtgaagt tggcatcgaa gcccatggat atgtctgtga
    28381 cgtgacagac gaggacggta tccaagccat ggtcaagcaa atcgaacaag aggttggtgt
    28441 cattgacatc ctcgttaata acgctggtat tatccgccga gttccaatgt gcgaaatgag
    28501 cgccgctgat ttccgtaagg tcatcgatat tgacttaaac gcaccattta tcgtttcaaa
    28561 ggcagttatt ccttctatga taaagaaagg gcatggaaag attatcaata tttgttcgat
    28621 gatgagcgaa ctgggacgtg aaacagttag cgcttatgct gctgctaaag ggggcttgaa
    28681 aatgttgacc cgcaacattg cgtctgaata cggtggagcc aatatccaat gtaacggaat
    28741 tggaccgggt tatattgcca ctcctcaaac agcacctctt cgtgagttgc aagaagatgg
    28801 ttctcgccac ccatttgacc agttcatcat tgcaaaaaca cctgctgcac gttggggaaa
    28861 tcctgaagat ttgatgggcc ctgctgtctt tctcgctagt gatgccagca attttgtcaa
    28921 tggccacatc ctatatgtag atggcggtat cttagcctac atcggaaaac aacctgagta
    28981 aaaatagaaa gaagatctta tgaaaatcgc attaatcaat gaaaatagtc aagctagcaa
    29041 gaatcacatt atttacgata gtctaaaaga agcgacagat aaaaaaggct accaattatt
    29101 taactatggt atgcgtggag aagaaggaga aagtcaatta acttatgtgc agaacggact
    29161 aatggctgcc atccttttaa atacaaaggc agttgacttt gttgttaccg gctgtggtac
    29221 gggtgtaggg gctatgcttg ctttaaacag cttccctggt gttgtctgtg gtctagcagt
    29281 ggacccaact gacgcttacc tttattctca aatcaatggt ggtaacgcct tgtctatccc
    29341 ttatgccaaa ggatttggct ggggggcaga actgaccctc aaattgatgt ttgaacgctt
    29401 atttgctgaa gaaatgggcg gtggctaccc aagagaacgt gtaatccctg aacaacgcaa
    29461 cgctcgtatc ttaaacgagg tgaaacaaat cacccacaat gatttgatga ccatccttaa
    29521 aacaatcgac caagacttcc tcaaagacac catctctggc aaatatttcc aagaatactt
    29581 ctttgaaaac tgccaagatg atgaagtcgc tgcttatttg aaagaagtat tagccaagta
    29641 aagctattct aaaccagaaa ggaactaatg gatgacgaaa atattactgt ttggcgaacc
    29701 attaattcga atctcaccat tagatgccac cagtatcggc gatcatgttg ccagttcgac
    29761 ttattttggc ggatcagaaa ttaacatcgc ttgtaatttg caagccctgg gtatctcaac
    29821 gaaagtcttt accgcactcc ctgccaacga gattggagat cgttttctca cattcttgaa
    29881 acagcaccaa atcgatacca gttcaatctg tcggcttggc gatcgaatcg gcctctacta
    29941 tttggagaac ggctttggtt gtcgtcaaag tgaagttttc tacgatcgta agcatacgag
    30001 tatcagccag attcggccaa acatgctaga tatggattct ctctttcagg ggattagcca
    30061 ttttcatttt agtggaatca ccgtagctat cggtcaagag gtccgtgcga tccttctcct
    30121 actcttggaa gaagccaagc gccgaggaat tgtcgtttca atggatctca atctgagaac
    30181 aaagatgatt tcagtcctag aagccaagta tgaattttct aagtttgcac gttttactga
    30241 ctattgcttc ggtattgatc ctctcatgat tgatgaccaa aatctagaga tgtttccaag
    30301 agacagtgct agcctagaag aggtggaaaa tcgcatgcga cttttaaaag aagcctatgg
    30361 tttcaaggcc attttccata ccctccgctc tagtgatgag caagacaaaa atgtctatca
    30421 agcctatgct ctggaagaac gatttgaaga gtctgtccaa ctaaaaactg cagtctatca
    30481 acgaattggt agcggggatg cctttatatc tggtgccctt taccaactac tccatcattc
    30541 ctccctaaaa actaccattg actttgcagt tgcgagcgca actctcaaat gcactcttcc
    30601 aggagaccat ctctccactt ccgcaactag tattgaaaat ttactggcaa atgcacaaga
    30661 tatcattcgt taggagaatt acatgaccaa atcagatacg attattgaac taaaaaaaca
    30721 gaaaattgtc gctgtcattc gaggaaatac aaaggaagaa ggactgcaag cctcgattgc
    30781 ttgtatcaag ggcggtatca aagctattga aatcgcctat accaatcagt atgcaggaca
    30841 aatcatcaag gaacttgtag acttgtatca ggacgatcag agtgtttgta tcggtgcagg
    30901 tactgtgctt gatgccgtaa ctgctagaga tgccattcta gccggagcaa attacgttgt
    30961 ttctccatct ttccatgctg aaactgcgaa aatgtgcaat ctctacagca caccgtacat
    31021 tccaggctgt attaccctca cagagatcac gactgcactt gaagccggta gtgaaatcat
    31081 caaactcttc ccaggtagtg ctctcagtcc agcatatatc tctgcagtca aggcaccgat
    31141 cccacaagtt tccgtaatgg taaccggagg agtcggtcta aacaacatcc ctcaatggtt
    31201 cgctgctggt gcagacgccg ttggaattgg tggcgaactc aataaactcg cttcccaagg
    31261 caactttgac cgcatcagcg agattgccca acagtatgtt acactcagat aaaatcataa
    31321 ccacccgtca aacgggtggt ttatcccaga gctataagcc caaattacca gccagcgcct
    31381 aaagacgctg gctttcacgt tgttcaagcc ttattgctct tgactcgtca cttgcctctt
    31441 taagagactt tggtattact taccactatc cctaaaggga tcctcatatt cttttacact
    31501 caatttatct agtgctatag tagattgaaa ctggaatagt acacctctac ttctaaaaca
    31561 ttgttaaaaa tcgatttgac tgtcctgatc gattttgtcc tgttcttatt tcattttact
    31621 atatatcata ctttactagt tctcaaattt tcatactcgt gaagaaatca tccactcgat
    31681 aatttcttta atcttgacta tatttcttaa ttgtggcttc attaagccct actggactta
    31741 cataataacc ttccgcccag aaatgccgat tcccaaactt gtacttgaga ttggcgtgtt
    31801 tgtcaaacat catgagtgca cttttgcctt ttaaataccc cataaaactt gaaacactta
    31861 gcctcgacgg aatactgact aacatgtgta catggtctgg cattaagtga ccctcgatca
    31921 tttcaacacc tttataacta cacaagcgat gaaatatttc gcctaaacta cttctatatt
    31981 gattatagat gacttttcgt ctatacttag gggtgaacac aatgtgatag aacacctcca
    32041 ctttgtgtga gataaactat gagccttttg tgccatattt tttctccttt cgctttacaa
    32101 ttggattgaa cacctttatt gtatcacgtt tggagttttt ttggtataac cttcgacgcg
    32161 cacccgcata gcgggtggtt gttttgtctc gcacctcacg gagcgagacg gactaatata
    32221 gtggagtgaa ataggatacg aacaaattga ttaggaaaat caaatgaatt tatagaaatc
    32281 ttttagcagt tatgacgtcc tattctagtt tcaaaacgct atagtcacat aatgaagtaa
    32341 aaaaggataa gtatcaactt atcctttttt aaaagaaaaa tccgaagata tttggccttc
    32401 ttcggatttt ttctattttc cacagtttca tgtaattcat ctagatgatg aacaaattag
    32461 ttgttctttc ctctacggaa taaataaaac gccccaagta gcaagaaccc tagacttgcc
    32521 aagattgact gaccttctcc tgtctgaggg agattctttt gatccgaatg gttcttttcc
    32581 tcttcagatt tttccttttc ttttgaattc tgtacttgtg gctgagctgc ttgctctagc
    32641 tttttaaaga cttcctgatc tggagctgat tcctgagttt caggattata gtaggcaatc
    32701 ttatattcat ccccttcttt tcgaatagta tagactccac gtttcaaaac ttggaattgg
    32761 ttggaaatag tagagacaga atcatcatat ttcacaatgc cccaaactcc ttgtttggca
    32821 tcataaacag actgaagggt ttcgttattt tcgatgaggc tactttctaa ctcttttatc
    32881 atttgattga aggtggcacg atccacgtta gggataagca tatagccata agaatctcca
    32941 ttttgcttat gagcctgact aatcgtaaga aattcatttt caacttcctt gtctgactgt
    33001 ccttcattga tatccttcca ggctcccttt tgcaaagcct tactcatgct gattgaactc
    33061 ttcttaaaga aaaagtaacc aatattcttt ttcgaatcgg acgattctaa aaagacactt
    33121 tgggtttcag gataatcctt ttcttgttct gtaagggagg cttctttatc attgacatag
    33181 actttatatg gattacttaa ttccagtttt ctctggtcga ttgtagttgc agcagtatct
    33241 gttgaagtgt tttggatatt gcttcctaaa aaggcaatct tatcctttag cataaaccag
    33301 ctcttatgag cagtcaatgt ttgattccag ttggtgaaat ccatggttgc tgtcgcattg
    33361 gcatcatcta gtttgctcgt tccaacgaaa gcagacggta aaactttacc tgtatcgcta
    33421 tccgctctct tagcatccgt ctctgttgta ccaggcatct tatatggatt aactgttggc
    33481 cagtagccat cgctatagtg actcaaatcg ccattgtaaa gatagaacat cccatcactc
    33541 gtataccaac cacgtttatt ttccttgttc atgtgttcgt aattcaaggt acgactggaa
    33601 aagagtgaca agccaaatcc aaaccctttc tctgcattgt acatggctgt tttatccatc
    33661 ttgttaaagg cagataggta acttgttctt ggaacacttg cgactcctgc atcacttaac
    33721 aaggattgca tcaaactgat atccttataa gtcttcaaat tcttaaagac atcataataa
    33781 ctatccgatt gaacaatggt cttcacaaga ctctgcaaac gttgtttggt ttctccttca
    33841 gacatatccg ctattcggtg aatccctctt agtacttcta ctgcggccac gtgcccctcg
    33901 ctatttgcac gactgatcga gcgtccacga ctcatatcca tcagctctcc attcaccagc
    33961 aaaggagcaa acgatttatc aatccagtgg tacatggttt gcattttatc tttatcgatt
    34021 ggattcttgg tcttttgaat gactggcaac agttgagaca ggccatcaat caaaacattc
    34081 ccataagcac ccgtataggc aacattggta tggtcgatat aggatccatc ttgataaaaa
    34141 ccttcacctt ggtctaccaa cttgaacact tgctcaatcg agcgaatggt agaagaaatt
    34201 tcttgatcat ccttacgcag taaaccagct attactttta ctcttcccat atcaactaag
    34261 tttccaccta gagccttaaa tgggttatca gtcgtctttc ggaaatgttc gggatctggt
    34321 acaaattttt caatcacatc tgtatatttt ttaatttcct catcagagaa gtattctttc
    34381 atcagagaca aggtattgtt gatggcacga ggtgtaccga tttcataatc ccaccagttc
    34441 ccaacaatgc tcttttcacg attgtagaca tgtttatgca tccattccat ggaatccctg
    34501 actgttcgaa cgacagtttc atcttgataa taacgagaag aaggattggt cacttgcttg
    34561 gccatctcct ccaatttccg ataagtggca gtcagatttg cagacatttt ataatttgaa
    34621 aatttttccc acaaataggt gcggtccgcc tgacttgaaa tactagatag gctatcagct
    34681 acctttcctt ccaattcctg gtttaatttg gccatctgtt catttttaga atcatagtat
    34741 tgattcccag cgatgatgcc attccagtca tccaaacggg ctgtgtatgc atccttaaca
    34801 gaggctagaa tcttcaaagg aatctttttc acttccttgc catctttact gacaatgaca
    34861 ttggttgtcc cttccttaag aggttctaaa attccatttt tgactgaagc aacgtcagga
    34921 ttttctacct tataagtata gtccgcaaga gaaaaaacat gtttttttcc aattggtaaa
    34981 tcaatctttt cctcaagctg tttatctgtt tgagaatcct cagaaagctg gtctgctacc
    35041 tctaccagct caatatcctt aaaggaaaca gtcccagttc ctgtttcata gaataactcc
    35101 agcttgattt tatcaacatc taaagtcggg ctatagtctg cttcaatggt ctgccagtcc
    35161 tttgttcctg acgtcgttgc agaattccac aatcgcttgt ccttaccact ttcctcaatg
    35221 atacgaacct tggcaatccc gactttatta tctgttttaa tcttgaaacg cagtttatac
    35281 tttttcttag cttcaatagg aaccatacgg tgaaccgctg cccttaattt ctcagggctt
    35341 gagatagtga tagccccatc cttagcctca atgactcgag ttgaggcatc tgcagaacta
    35401 ttcttctggt ctacccaagc tgaccacccc tgagctttgc ttcctgtcca cggactgctt
    35461 ccgtcctccg tctgaccaaa atcaccattt tcaactaaat tttttgtatc cctttgctga
    35521 tactgaactt ccttctggct ttgctgaatg gtattcgtag tcgtttcttc cgctccaact
    35581 gtcatataat ggtttaataa aaatcctgat aaaacaagtg aagacaaact cacaatgagc
    35641 ttctttgttt ttgtttgcat gtatacactc caataaacat attgtaaaat caataggaac
    35701 taaatctcaa attagagatt tatagcaaca aagtaaaccg gtttaattaa atcataccat
    35761 ttttaaagtt atttgtaaac ggtttctatc atattgaggg cttattcttc aacaatatct
    35821 tccatccgac tcgacgaaaa aagtaaaaac tgttacaatg tagaaaaaga aaagaggccc
    35881 tcatgacttc actttatgat ttttccgttt tgaaccaaaa caaccaagca actcccttgg
    35941 atagctatcg tggcaaagtt ctcttgattg tcaacactgc tactggatgt ggtttaacgc
    36001 cccagtacca gggacttcaa gaactctatg aacgctatca agatcagggc ttcgaaatat
    36061 tggatttccc ttgcaatcag tttatgggac aagcacccgg cagcgcagag gaaatcaaca
    36121 ccttctgtag cctacatttt caaaccacct tcccacgttt tgccaagatt aaggtcaacg
    36181 gtaaggaagc agaccctctc tatgtctggt taaaagacca taaatctggc ccactaggaa
    36241 aacgaatcga atggaatttc gctaagtttc tcattggtcg tgatgggcaa gtctttgaac
    36301 gcttttcttc aaaaacagac ccaaaacaaa ttgaagaggc gatacaaact ctactataat
    36361 tcacaatctc actatgatta ggtttccttt aacctgatga atagtgagat tttttgattg
    36421 gctttgactt aaatagaaaa acaccccatg atatgaaaca tgaagtgttg taaagtctat
    36481 gttgtaggtg cttatttcac attttcaatg tgaccagtga taacgaatac catacagaat
    36541 cttcatatac actaaacaaa tgactttcta attatttcaa ttagttttgg ctagtaaata
    36601 tcatttccaa caaacgccct ctcaattcct tatcctgatg atgcaagata ttcattaagt
    36661 catgagagtt tttcgcattg atgaattgat ttaacaatct atcttttaat tcatatggaa
    36721 gagaagctgt ctttagtagt ctaaaaactt cgtcatttaa agatgtcctt ttattatctt
    36781 tccattcaaa tttagctgta tcattcttat ttggcaattc aattatagac acattcgttc
    36841 ctttaaaatg aattctatgt tttctattgc ttggaacgat actagaatct ccttgtaatg
    36901 ctaactctac cattcccatt tcccaatcga ttgataatct tgttttatat ctttgaccat
    36961 tttgatcttc aagcatttca aaagaatgtt gtttccctgg gaatacatac caatctacaa
    37021 cctcaggtaa atcaacaccc atacctatct cagaaccaac caagggaatg attgcaccac
    37081 tttttgcaaa cacaggcgta gtcgagatgt ccctataaac acttaacttc acaccacctg
    37141 tgtatttttt ctctgaaaag aagtcatacc attcaccttc agggaaccat acatctactt
    37201 ttgcagattg gaatgtcaaa tccatctttt ctacaatggg agccaccatc agttctgttc
    37261 caaaaaagta ttggtttgga acattatagc tctcatcatt ctctggatag aaataataga
    37321 ttggactgat taatggggca ccttcctcat gtgtctttac attcatggta tatagataag
    37381 gaatcatctg atgtctcaaa cgaaggtatt tcttcataat cttagatgtt gtttctgaaa
    37441 aaaaccaagg ttctttacta ttaaaaggac ttctagaact atgtaatcga gtaatcggac
    37501 taaaaacacc aaactgtagc catctagttt gtagctcttc gtcataatcc cccaacatat
    37561 gtccaccgat atcatgactc caccaactat aaccgatatt agatgctgtc gctgtaaaat
    37621 agggttgaaa tcttaaggaa ttccaactga taatagtatc ccctgaaaaa ccaacagggt
    37681 agcggtgact accaggacct gcatatcttg ataaaatcaa accaccttct gcatttttac
    37741 aactatcctg atagtgataa tggtttaaaa gccaaagtgg atctagcatg ccttgtgtcc
    37801 cctgttgcca gtcaatccac caaaaatcta ctccctgctt ttctagctca taatgaacat
    37861 ctttaaagta ggcttcccta aaagagggat taaaaaaatc aaaaatagca ggttcttcta
    37921 gttctacatt taaccccaac cgttttgcga tttgaggata agcttcttca taagcccgta
    37981 tcccatcagc aggatggaca tttaaggaga gttttaactt tctatcatga agttgttgca
    38041 ataactgttc tggatttggt attaagtttc tattccaact atatcctgtc cagccacttc
    38101 caaaacgagc tggaatgtca gttatatgcc aatccatatc taacacaccg atagataatg
    38161 gaattttctc tgttgcaaat ctgtctatta aatccaagta ttcatccgac gtataaggcc
    38221 aatatctact ccaccaattg cctaaagcat atcttggcaa caagggtgtt gaaccagtca
    38281 aatggtaaaa gtctctgatt gctcctctat aatcatgccc ataggcaaag aaatacaggt
    38341 caatttgatt ttctctctca atataaccag attgttcatc ccaaataaat ccttgagaat
    38401 catccaataa ggctatacca tttcggctaa taattccatc ttctaacgag attgctccat
    38461 ctgccttatc cagagtccga gctgttcctt ttaacgtttc aatagattca ccaaaatacc
    38521 agcgactacc atatacggca aaatttcctt ttaattctat aaataaattt tcggcgttaa
    38581 attctccttt attaaagtgc agatgaaaat agtccgtcat aatatctagt acgtttgatg
    38641 tctcgatata atctaacgaa acttggccaa aatctctatt atagataagt tgtgtcgttc
    38701 tatcctcaaa acttccagtt tgagagtatt ctaaccttac tagcttgtct gttaatacag
    38761 agattcgata aaactctccc ttaaaaattt tcaatttgtt ttcctccttt tatggtagca
    38821 taaaaacaga acgcaccatt tttgatgcgt ttttcattat tctgaatgca atgttctatc
    38881 tgttatatct atgacaaata atagtcaatt gaaaaaatgt agtggacaaa atatctttta
    38941 acaaaccaag agtttattaa agagttatca cttttcaact tttctaagct tatgcagttg
    39001 tgaaacaaac tacttttaaa ctattaacta agataggatt gataaataat ttcaaactct
    39061 tactagcaat catacgatat tcaagctcgc gtgctttttt ccttcctgct tatttcttag
    39121 aactgaagaa cccggatcgg tatataaatt atccggatca acatagtcat aagactcata
    39181 acagttgcgc ttcattaagt catccccaga gcaagagctt catctcgtaa tttttcaaca
    39241 tcactaaccg taggtcgcca tccttcaatc atatttgtac ttaaagcata ccaaacactc
    39301 ttaaaaacgg atcggttttc aaaagctatt cccatgattg tcatcttttc tttatctata
    39361 tctaaggaca tatgctacct cctttagata cattatacca tgtttctctg tagcttttaa
    39421 aaattttatt ttgtttgtca tatctaagtt ttcagtacgc ttatcctatt ttataagcct
    39481 caaacccaaa tataaaacgc attaaccatc tgataatatg cgttttattg tggaaacatt
    39541 tcccattttc tctatttcta atgacttact tcaacttctg acttcatgga aaaaacaaca
    39601 aaaatatgaa ctggcaaagt ttggtatcat cagtaatcca gaacaattca tctttactta
    39661 catagatacc aaaggcaata tcaataaacc tttacattct gactatctca ataataagat
    39721 gaaaactatc agaaaacgac acccagagct tacacacgct acgccacaca aactacgtca
    39781 taccggagca acgctcgcta aacaagcagg aatgagctta gaagccattt ctgaagctct
    39841 aatacatagc gacacaggta caacacagat ttatgtcaat acttctaatg tagtccctat
    39901 ggccgtcggt gaatttgcct tacagtctct aaaacaataa ggtgaaaata aggtaaactt
    39961 tgaggtgaac tttttcaaaa aacataaaac atactaagat tagtagtgag gaaatctccg
    40021 acgggagaaa gtactcacta ctttttcttt gtgataaagt agaggtgtct tgttaagtcg
    40081 agggttcttt tgacgctaga cgtcgtacat aaaactggca agacgcctgt tttaggtgga
    40141 attttatcac agtatgtggg acgcaaaacg tcgtgtattg aaaatgaaat cactaaaaca
    40201 aggaatcatt caagacaaag aggtaatatc atgcgagtcg tatttgggat tgatgtcagt
    40261 aaggcaagtt cagaagtggc tattttagtc aatggtgata aggttcatgg ctacaccata
    40321 cctaatgatt ctatcggctt caatcggcta cttggtgact tgaaaactgt ctctaatcct
    40381 gagattgtct tcgaagcaac aggtgtctat tctcgtcgtc ttcaagcctt tcttgaagat
    40441 aatagctacg cttatacacg gttaaatcct ctagctgcta aaaagcaact tgacagctta
    40501 cgtgttcgta aaacagacaa aatcgatgcc gagaaattag cacaatctca gtttgttctg
    40561 aatcgtcaac caacttacgt ccaagaggaa gtctatcaag agttacgtga cctaagccgc
    40621 ttctatcaaa atctgactga agatattgtt cgagctaaga accgtctcca caaggtctta
    40681 caagtcacgt ttccagagtt ggaaagcctc ttgtcctcac caactggcga gcaatactgg
    40741 aatttagtca tagcttttcc ttgtaaggaa atggtgctag agctgagcag ggatgattta
    40801 tcaactgtca ttcgaaactc gacttcaaaa cgtatctctg acaagcgggt aacttatttg
    40861 actgagaagc ttactgatct agctcacaaa tcttattgtg ctgtcaagaa aacctctcca
    40921 atgcttgaag aggtccgtta ctatgcccaa gaattgcttc gactttctga acgcagacaa
    40981 gttgttctag acgaaatggt ttccttagct cagcctttgc cagaatatga gattcttctc
    41041 tctattcctg gtatcgcaga aacaacggtg acatcactca ttggcgaatt gggtgatatt
    41101 cgtcggtttc agtctgccaa tcaaatcaat gcctttatcg gcatcgacct gcgtcactat
    41161 gagtctggga acttccttgc caaggaacac atcaccaaac gagggaatcc ttatgccaga
    41221 aagattcttt tcaagtgtat tcacaacatc gcttcagcta gtcataccaa tccttgtcat
    41281 atcgcagact tttatgagaa acgaaaaaga caatcgcaaa cgacttccac caagccacat
    41341 acgattgcct ctatacatcg tcttataagg acaatgtatt acctcattac gcataataag
    41401 atttacgact ataacttagc cacaagtcaa taaacttatc tatgccacat tattctaaca
    41461 ccttgttctt aaaaaagcta gataaggtgt tccttttgtg tgcattttta tcgctactta
    41521 gcacagaaaa agaggacaat aactgatagt tagctactgc ccttaattta ttttttcttt
    41581 aaatcctgga gacgcttgac aaatggtaga aaaaaagcac ccatgaggta aactcttgag
    41641 tgctttgaaa cgttgatata attgtattga ttaacgtttt gaggcaactg accttgtcag
    41701 gttgcagcca catttgtaag cgactaaagt cgcaacaact gtgtcaattg caccaattta
    41761 gtaagaaagt ataaaaaaag aacaccccga aaggtgctct gtataagtat agtaattctt
    41821 tcgaattaac gtttactaaa ttgtgatgct ttacgagctt tcttaagacc tggtttctta
    41881 cgttcaactt tacgtgagtc acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag
    41941 tctgggtcta cttgaagaag ggcacgagcg ataccgtgac ggatagctcc tgattgacca
    42001 gcgtatccac cacctacaac gttaacgaaa acgtcgtatg aacctacagt tgaagtaact
    42061 gcgaatggtt ggttgatgac aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct
    42121 tttttgttaa cagtgatttt accagttcct ggaacaaggc gaacgcgtgc aacagcgttt
    42181 ttacgacgtc cagtacctgc atattgtgct tgtgacatac tttatttgtt cctttcctta
    42241 gataagtcct gaaatgtcaa gaacttctgg ttgttgtgca gcatgagtgt gctcagctcc
    42301 aacaaatact ttcaacttca taccttgagc gcgtccaagt gtattgtgtg gaagcatacc
    42361 tttaactgat ttctcaatca aacgtactgc atttttagaa cgaagttcac ctgcagagat
    42421 ttgtttcaat ccacctgggt ggtttgagtg agtgtagtag attttatcag ttgctttttt
    42481 accagtcaat ttaacttttt cagcattgat aacaatcacg aagtcacctg tatcagtgtg
    42541 tggtgtaaat gttggtttgt tttttccgcg aagtacgcta gcaactactg cagaaagacg
    42601 tccaagtggt acatcagttg cgtcaactac gtaccattta cgttcaactt ggcctggttt
    42661 agccataaat gttgttttgt tcatgatttc tcctatatat agttcgattt ttgtttacgg
    42721 tgatgggtgt tccttcccat cgaaaagtat ttggaaggtt ccggggcctt tcaaatgggg
    42781 taaacaatac cgcctactat cataccaaat ttcaccccta aaagtcaata gatatgaaaa
    42841 atatttttcg gtaacttctc aaagtaactg a
//
LOCUS       DAVKJU010000011        40286 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVKJU010000011 DAVKJU010000000
VERSION     DAVKJU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.011"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            5..75
                     /locus_tag="VW191_000486"
     tRNA            5..75
                     /locus_tag="VW191_000486"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Gly,seq:tcc)
     gene            110..183
                     /locus_tag="VW191_000487"
     tRNA            110..183
                     /locus_tag="VW191_000487"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Ile,seq:gat)
     gene            266..355
                     /locus_tag="VW191_000488"
     tRNA            266..355
                     /locus_tag="VW191_000488"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:302..304,aa:Ser,seq:tga)
     gene            369..442
                     /locus_tag="VW191_000489"
     tRNA            369..442
                     /locus_tag="VW191_000489"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Met,seq:cat)
     gene            446..518
                     /locus_tag="VW191_000490"
     tRNA            446..518
                     /locus_tag="VW191_000490"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:479..481,aa:Phe,seq:gaa)
     gene            531..611
                     /locus_tag="VW191_000491"
     tRNA            531..611
                     /locus_tag="VW191_000491"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Tyr,seq:gta)
     gene            616..686
                     /locus_tag="VW191_000492"
     tRNA            616..686
                     /locus_tag="VW191_000492"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:648..650,aa:Trp,seq:cca)
     gene            701..773
                     /locus_tag="VW191_000493"
     tRNA            701..773
                     /locus_tag="VW191_000493"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:734..736,aa:His,seq:gtg)
     gene            784..855
                     /locus_tag="VW191_000494"
     tRNA            784..855
                     /locus_tag="VW191_000494"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:816..818,aa:Gln,seq:ttg)
     gene            865..948
                     /locus_tag="VW191_000495"
     tRNA            865..948
                     /locus_tag="VW191_000495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:899..901,aa:Leu,seq:caa)
     gene            1055..2332
                     /locus_tag="VW191_000496"
     CDS             1055..2332
                     /locus_tag="VW191_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEU9623315.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKFLRRQFSKKNLTDEEETMIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2408..3892
                     /locus_tag="VW191_000497"
     CDS             2408..3892
                     /locus_tag="VW191_000497"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU9623316.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     NKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFVAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTDDATKTVIASTASPYKFPVVAVEALTGKVGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVRHKTIVAAADMQVAVEAYLGL"
     gene            3953..5233
                     /locus_tag="VW191_000498"
     CDS             3953..5233
                     /locus_tag="VW191_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9623317.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMNLKG"
     gene            5237..5857
                     /locus_tag="VW191_000499"
     CDS             5237..5857
                     /locus_tag="VW191_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9623318.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6023..7165
                     /locus_tag="VW191_000500"
     CDS             6023..7165
                     /locus_tag="VW191_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9623319.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQTSPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7571..7909
                     /locus_tag="VW191_000501"
     CDS             7571..7909
                     /locus_tag="VW191_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEU9623320.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7920..8288
                     /locus_tag="VW191_000502"
     CDS             7920..8288
                     /locus_tag="VW191_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEU9623321.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYNALLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            8395..9005
                     /gene="pcp"
                     /locus_tag="VW191_000503"
                     /pseudo
     CDS             8395..9005
                     /gene="pcp"
                     /locus_tag="VW191_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(9066..9923)
                     /locus_tag="VW191_000504"
     CDS             complement(9066..9923)
                     /locus_tag="VW191_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEU9623322.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYQA
                     VLARKRPKA"
     gene            10053..11195
                     /gene="tgt"
                     /locus_tag="VW191_000505"
     CDS             10053..11195
                     /gene="tgt"
                     /locus_tag="VW191_000505"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU9623323.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11293..13110
                     /locus_tag="VW191_000506"
     CDS             11293..13110
                     /locus_tag="VW191_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEU9623324.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPSYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13263..14414
                     /gene="nagA"
                     /locus_tag="VW191_000507"
     CDS             13263..14414
                     /gene="nagA"
                     /locus_tag="VW191_000507"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEU9623325.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14577..15635
                     /locus_tag="VW191_000508"
     CDS             14577..15635
                     /locus_tag="VW191_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEU9623326.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15786..16151
                     /locus_tag="VW191_000509"
     CDS             15786..16151
                     /locus_tag="VW191_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEU9623327.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16227..17168
                     /locus_tag="VW191_000510"
     CDS             16227..17168
                     /locus_tag="VW191_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEU9623328.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17116..18132
                     /locus_tag="VW191_000511"
     CDS             17116..18132
                     /locus_tag="VW191_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU9623329.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVSRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18134..18460
                     /locus_tag="VW191_000512"
     CDS             18134..18460
                     /locus_tag="VW191_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU9623330.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18453..18857
                     /locus_tag="VW191_000513"
     CDS             18453..18857
                     /locus_tag="VW191_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEU9623331.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSSSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18862..19122
                     /locus_tag="VW191_000514"
     CDS             18862..19122
                     /locus_tag="VW191_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623332.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19085..19546
                     /locus_tag="VW191_000515"
     CDS             19085..19546
                     /locus_tag="VW191_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9623333.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQTMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19524..19916
                     /locus_tag="VW191_000516"
     CDS             19524..19916
                     /locus_tag="VW191_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEU9623334.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKIRREA"
     gene            20072..20666
                     /locus_tag="VW191_000517"
                     /pseudo
     CDS             20072..20666
                     /locus_tag="VW191_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            20727..21680
                     /locus_tag="VW191_000518"
     CDS             20727..21680
                     /locus_tag="VW191_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9623335.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELEQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQVNHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLTKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21731..22921
                     /locus_tag="VW191_000519"
     CDS             21731..22921
                     /locus_tag="VW191_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU9623336.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23070..23441
                     /gene="rnpA"
                     /locus_tag="VW191_000520"
     CDS             23070..23441
                     /gene="rnpA"
                     /locus_tag="VW191_000520"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU9623337.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23416..24240
                     /locus_tag="VW191_000521"
     CDS             23416..24240
                     /locus_tag="VW191_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU9623338.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24259..25245
                     /locus_tag="VW191_000522"
     CDS             24259..25245
                     /locus_tag="VW191_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEU9623339.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSRTFYVTINVNDYVEHRAEVLQTYAQKL
                     ATRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25296..25919
                     /locus_tag="VW191_000523"
     CDS             25296..25919
                     /locus_tag="VW191_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEU9623340.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLEIPTVKGIYYLTYSDGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26050..27507
                     /locus_tag="VW191_000524"
     CDS             26050..27507
                     /locus_tag="VW191_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9623341.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFIFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27530..27811
                     /locus_tag="VW191_000525"
     CDS             27530..27811
                     /locus_tag="VW191_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9623342.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27888..28373
                     /locus_tag="VW191_000526"
     CDS             27888..28373
                     /locus_tag="VW191_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9623343.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28390..29055
                     /locus_tag="VW191_000527"
     CDS             28390..29055
                     /locus_tag="VW191_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEU9623344.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29059..29922
                     /locus_tag="VW191_000528"
     CDS             29059..29922
                     /locus_tag="VW191_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEU9623345.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29924..30628
                     /locus_tag="VW191_000529"
     CDS             29924..30628
                     /locus_tag="VW191_000529"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEU9623346.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYRAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30807..32480
                     /locus_tag="VW191_000530"
     CDS             30807..32480
                     /locus_tag="VW191_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9623347.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFALIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32592..33683
                     /gene="ulaG"
                     /locus_tag="VW191_000531"
     CDS             32592..33683
                     /gene="ulaG"
                     /locus_tag="VW191_000531"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEU9623348.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33797..35773
                     /gene="tkt"
                     /locus_tag="VW191_000532"
     CDS             33797..35773
                     /gene="tkt"
                     /locus_tag="VW191_000532"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9623349.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35890..36189
                     /gene="yajC"
                     /locus_tag="VW191_000533"
     CDS             35890..36189
                     /gene="yajC"
                     /locus_tag="VW191_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU9623350.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            36235..36663
                     /locus_tag="VW191_000534"
     CDS             36235..36663
                     /locus_tag="VW191_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEU9623351.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36665..37075
                     /locus_tag="VW191_000535"
     CDS             36665..37075
                     /locus_tag="VW191_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623352.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            37375..40026
                     /gene="adhE"
                     /locus_tag="VW191_000536"
                     /gene_synonym="adhC"
     CDS             37375..40026
                     /gene="adhE"
                     /locus_tag="VW191_000536"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEU9623353.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELIKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12187 a         7226 c         9100 g        11773 t
ORIGIN      
        1 tttagcgggt gtagtttagt ggtaaaacta cagccttcca agctgttgtc gcgagttcga
       61 ttctcgtcac ccgctttgaa ctttgttctt tgtaccaagt ttttgacttg ggcgcgtagc
      121 tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt ccactcgtgc
      181 ccatagtgtt tagtccatta ctaggggatt ggaatattat ctgttcacta agaggacacg
      241 ggcttgttcc cgtataaact attttggagg attacccaag tccggctgaa gggaacggtc
      301 ttgaaaaccg tcaggcgtgt aaaagcgtgc gtgggttcga atcccacatc ctccttttat
      361 attattaacg cgggatggag cagctcggta gctcgtcggg ctcataaccc gaaggtcgta
      421 ggttcaaatc ctgctcccgc aataaggctc ggtagctcag ttggtagagc aatggattga
      481 agctccatgt gtcggcggtt cgattccgtc tcgcgccatt tatatatttt ggaagggtag
      541 cgaagaggct aaacgcggcg gactgtaaat ccgctccttc gggttcgggg gttcgaatcc
      601 ctccccttcc attttacggg catagtttaa aggtagaact aaggtctcca aaaccttcag
      661 tgtgggttca attcctactg cccgtgttaa tagaattatg gcgggtgtgg tgaagtggtt
      721 aacacaccag attgtggctc tggcatgcgt gggttcgatc cccatcactc gcctatttta
      781 tattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga
      841 atccagctac cccagttact atttgccggc gtggcggaat tggcagacgc gctggactca
      901 aaatccagtg tccgcaagga cgtgccggtt cgaccccggc cgccggtata gtatagtgtt
      961 aggaacgttg ttattcttcg ttcctttttt atattatttt tggtataatt atagttattc
     1021 aaattttatt tagattaaga aagtgtaggg gagtatgtct tgttctatcg atttattaaa
     1081 acatcggtat ttgaaaaata ttaaagaaaa tcctgaattg tttgtcggaa ttgagttgga
     1141 gtatcctgtt gcaagtttag aaggggatgc tacagatgtt gaagttataa aggatctatt
     1201 tcattattta gtttctactt tggatctcac cgtagcaaag gtagatgatt ttggcaatct
     1261 gatccagtta gtagatccga taagtcagga tgctatttta tttgaagttt cctatacaac
     1321 gattgagttt gcatttggta aggctgaaac gattcaagag gtcgaaaatc gtttcaataa
     1381 ttatatgaat gtaattcaga gaaagttagc tgaatcaaat catgctattg ttggctgtgg
     1441 tatccatccc aactgggata aaaatgagaa ttgtccagtg gcttatccac gctatcagat
     1501 gttgatggat tatttgaatt tgagtagaaa tattattaaa tcagatttac atcatttccc
     1561 tgaatatggt acttttatct gtgggagcca ggttcagctg gatatttcaa aaaccaacta
     1621 cttgcgggtg attaatgctt ttactcaaat tgaagcggct aaggcttatt tatttgcaaa
     1681 ctctgagttt tcgggtgcgg attgggatac gaaaatttca agggatattt tctgggaaga
     1741 atctatgcat ggtatctatc cagagaatgt tggggtcaat gctagactcc ttaatgatga
     1801 agctgatttt tttgactatc taaatcattc tgcgattttt actgcggaac gtgatgggca
     1861 gacctattat ttttatccta ttcaggctgg ggactatttg gctacgcctg aaatccaagc
     1921 atttgctctg aatggggatg aggttattat ttacccccaa gagaaggatt ttgaaactca
     1981 tcgtagttac cagtaccaag acttaacgac tcgaggaaca gttgagtttc gtagtgtgtg
     2041 tacacagcca cttgatagga cttttgcttc tgcagctttt cacttgggat tattggttaa
     2101 tttagacaag ttagaagctt acttagaaac agcacctttc tttaaagtat ttggttatga
     2161 ttacaagttt ttaaggagac aattttctaa gaaaaatctt acagatgagg aagaaactat
     2221 gattattgaa ttttccaaag acttactcct actagctgag gagggactag tggtgagaaa
     2281 taaggaagaa atgacctatt tacagccttt gagagaagaa ttgagcctat aatttctctt
     2341 ataaagggag aattttctga aaaatcatga tataatggac gagactatag ataaaggata
     2401 gagagtaatg acattagttt atcaatcaac gcgtgatgcc aacaatacag taactgccag
     2461 ccaagcaatt ttgcaaggtt tggcgacgga cggcggtttg tttacaccgg ttacttatcc
     2521 aaaggtagat ttgaacttta acaaattgaa agatgcttct taccaggaag ttgctaagct
     2581 agttttgtca gcatttttag atgactttac agctgaggag ttggactact gtatcaacaa
     2641 tgcctacgat agcaaatttg atactccagc tattgcacca ttagtgaaat tagatgggca
     2701 atacaatttg gaacttttcc atggttcaac gattgccttt aaggatatgg ccttgtctat
     2761 tttgccatac tttatgacga ctgctgctaa gaaacatggt ttggagaaca agattgttat
     2821 cttgacagcg acatctggtg acacggggaa agctgctatg gcggggtttg cggatgtgcc
     2881 tggtactgag attatcgtct tttatccaaa ggatggtgtc agcaagattc aagagttaca
     2941 aatgaccact caaactggcg acaatactca tgttattgct attgatggta actttgacga
     3001 tgcgcaaaca aatgtgaagc acatgtttaa cgacgtggct cttcgtgaaa aattgactac
     3061 caacaagttg caattttcat cagctaactc tatgaacatt ggtcgtctgg tgccacaaat
     3121 tgtttattat gtttatgctt acgctcaatt ggtcaagact ggtgagatcg tggctggtga
     3181 taagattaac ttcacagtac caacaggaaa ctttggaaat atcttggctg ccttttatgc
     3241 caaacaaatc ggtctcccag ttggtaaatt gatctgtgct tcaaatgaca acaatgtttt
     3301 gacagacttc ttcaagacac gtgtttacga caagaaacgt gagtttaagg taacaaccag
     3361 cccatctatg gatatcttgg tatcttcaaa cttggagcgt ttgattttcc atcttttggg
     3421 gaatgatgcg gttaagacgg ctgaactcat gaatgccttg aatacacaag gacaatatga
     3481 actgactgat tttgatgcag agattctgga gctctttgta gctgaatatg cgactgagga
     3541 agaaacagcg gcagaaatca agcgtgttta tgagttagat tcttatattg aggatccaca
     3601 tacggcggtt gcctcagcag tttatagaaa ataccaagcg gttactgatg atgcgactaa
     3661 gacagtgatt gcttcaacag ctagtcccta caagttccca gtagttgcag tagaagcgct
     3721 aacagggaaa gttggcttga ctgattttga agctttggct caattacatg aaatctcagg
     3781 cgttgcagtg ccaccagcag ttgatgggct tgagacagct cctgttcgtc acaagacaat
     3841 agtggcagct gctgacatgc aagtagcggt tgaggcttat ttaggacttt aagatagagg
     3901 gagtaaactc ggttgggaaa ccaactgagt ttcttttcat caggaggaga gattgtttaa
     3961 gaaaaataaa gacattctta atattgcatt gccagctatg ggtgaaaact ttttgcagat
     4021 gctaatggga atggtggaca gttatttggt tgctcattta ggattgatag ctatttcagg
     4081 ggtttcagta gctggtaata ttatcaccat ttatcaggcg attttcatcg ctctgggagc
     4141 tgctatttcc agtgttattt caaaaagcat agggcagaaa gaccagtcga agttggccta
     4201 tcatgtgact gaggcgttga agattacctt actattaagt ttccttttag gatttttgtc
     4261 catcttcgct gggaaagaga tgataggact tttggggacg gagagggatg tagctgagag
     4321 tggtggactg tatctatctt tggtaggcgg atcgattgtt ctcttaggtt taatgactag
     4381 tctgggagcc ttgattcgtg caacgcataa tccacgtctg cctctctatg ttagtttttt
     4441 atccaatgcc ttgaatattc ttttttcaag tctagctatt tttgttctgg atatggggat
     4501 agctggtgtt gcttggggga caattctgtc tcgtttggtt ggtcttgtga ttttgtggtc
     4561 acaattaaaa ctgccttatg ggaagccaac ttttggttta gataaggaac tgttgacctt
     4621 ggctttacca gcagctggag agcgacttat gatgagggct ggagatgtag tgatcattgc
     4681 cttggtcgtt tcttttggga cggaggcagt tgctgggaat gcaatcggag aagtcttgac
     4741 ccagtttaac tatatgcctg cctttggcgt cgctacggca acggtcatgc tgttggcccg
     4801 agcagttgga gaggatgatt ggaaaagagt tgctagtttg agtaaacaaa ccttttggct
     4861 ttctctgttc ctcatgttgc ccctgtcctt tagtatatat gtcttgggtg taccattaac
     4921 tcatctctat acgactgatt ctctagcggt ggaggctagt gttctagtga cactgttttc
     4981 actacttggg acccctatga cgacaggaac agtcatctat acggcagtct ggcagggatt
     5041 aggaaatgca cgcctccctt tttatgcgac aagtataggt atgtggtgta tccgcattgg
     5101 gacaggatat ctgatgggga ttgtgcttgg ttggggcttg cctggtattt gggcagggtc
     5161 tctcttggat aatggttttc gctggttatt tctacgctat cgttaccagc gctatatgaa
     5221 cttgaaagga taggaaatgc aaaaaacagc ttttatttgg gatttagacg ggactttatt
     5281 ggactcttac gaagcgattt tatcagggat tgaggagact tttgctcagt tttctattcc
     5341 ttatgataag gagaaggtga gagagtttat cttcaagtat tcggtgcaag atttgcttgt
     5401 gcgggtggca gaagatagaa atctggatgt tgaggtgcta aatcaggtgc gtgcccagag
     5461 tctggctgag aagaatgctc aggtagtttt gatgccaggt gcgcgtgagg tgctagcttg
     5521 ggcagacgaa tcaggaattc agcagtttat atatactcat aaggggaaca acgcttttac
     5581 cattctcaag gacttggggg tggaatccta ttttacagag attttaacca gtcagagtgg
     5641 ctttgtgcgg aagccaagtc cagaagcggc tacctatctg ctagataagt atcagttgaa
     5701 ttctgataat acttattata taggggatcg gactctggat gtggaatttg cccagaatag
     5761 tgggattcaa agtatcaact ttttagagtc tacttatgaa gggaatcaca ggattcaagc
     5821 gttagcagat atttcccgta tttttgagac taagtgataa aaagattgtg tcagttttgt
     5881 gacagagacc taacaaacta tttcaagtaa cctagtttgt tacaaggaat agacagttct
     5941 gttaaatagg cccgagaggg ctttttttct acattttttg tgttatcata gacaggtact
     6001 catttgaaag gaatttgaaa gaatgaagaa aagaatgtta ttagcgtcaa cagtagcctt
     6061 gtcatttgcc ccagtattgg caactcaagc agaagaagtt ctttggactg cacgtagtgt
     6121 tgagcaaatc caaaacgatt tgactaaaac ggacaacaaa acaagttata ccgtacagta
     6181 tggtgatact ttgagcacca ttgcagaagc cttgggtgta gatgtcacag tgcttgcgaa
     6241 tctgaacaaa atcactaata tggacttgat tttcccagaa actgttttga caacgactgt
     6301 caatgaagca gaagaagtaa cagaagttga aatccaaaca cctcaagcag actctagtga
     6361 agaagtgaca actgcgacag cagatttgac cactaatcaa gtgaccgttg atgatcaaac
     6421 tgttcaggtt gcagaccttt ctcaaccaat tgcagaagct ccaaaagaag tagcatcaag
     6481 ttcagaagtt acaaagacag tgattgcttc tgaagaagtg gcaccatcta cgggcacttc
     6541 tgtcccagag gagcaaacgg ccgaaacaag cagtgcagtt gcagaagaag ctcctcagga
     6601 aacgactcca gctgagaagc aggaaacaca aacaagccct caagctgcat cagcagtgga
     6661 agcaactaca acaagttcag aagcaaaaga agtagcatca tcaaatggag ctacagcagc
     6721 agtttctact tatcaaccag aagaaacgaa agtaatttca acaacttacg aggctccagc
     6781 tgcgcccgat tatgctggac ttgcagtagc aaaatctgaa aatgcaggtc ttcaaccaca
     6841 aacagctgcc tttaaagaag aaattgctaa cttgtttggc attacatcct ttagtggtta
     6901 tcgtccagga gacagtggag atcacggaaa aggtttggct atcgacttta tggtaccaga
     6961 acgttcagaa ttaggggata agattgcgga atatgctatt caaaacatgg ccagccgtgg
     7021 cattagttac atcatctgga aacaacgttt ctatgctcca ttcgatagca aatatgggcc
     7081 agctaacact tggaacccaa tgccagaccg tggtagtgtg acagaaaatc actatgatca
     7141 cgttcacgtt tcaatgaatg gataaacccg acttgataac atcattttga cgaatgagat
     7201 ctagctttcg tgatggaaag cgattctcgt tcgttttttc tttgtcatac tcttcgaaaa
     7261 tctcttcaaa ccacgtcagt tttatctgaa acctcaaagc tgtgctttga gcaacctgcg
     7321 actagctttc tagtttactc tttgattttc attgagtatc aatttgaatg gaaaatggaa
     7381 agttattatc ttgtaataat ccaagcaaca ttcttgcaat ctattttact ttatatcaca
     7441 attgattggt caactattga taagtcaatg gataggagga agaaatgata gagattcaag
     7501 atttactgta tcaactccgc ttgtctgagc aagcgagtac gcaattgttt gaaaaaggct
     7561 tgggattagt ttgacacggt atcagatttt actgtttttg ctggagcatt ctccttgtaa
     7621 ccaaatggcg gttcaggagc gtttgaaaat tgatcaggct gctttgacac ggcatttcaa
     7681 gattttggaa acggaaggtt tggtggagcg tcatcgtaat cctgaaaatc agcgggaagt
     7741 gttggtagag gctgcgaagt atgccaagga gcagttagtg gtgaatcccc ctctgcaaca
     7801 tatcaaggtt aaggaagaga tggaaagtat cttaacagag tttgagagaa cagaactcag
     7861 ccgtttatta aataaattgg ttttgggtat tgaaaatata gaaatttaag tagaaataga
     7921 tgtcaattat tacaattatt ttaacaacga tcgttgcttt ggagcatttt tacatttttt
     7981 atttggaaag tattgccacg caatcagatg cgactagtcg tgtatttaat atggaaaagg
     8041 aagaattggc tcatccgtca gtaagttcat tgttcaaaaa tcaaggaatt tataacgctc
     8101 tgctaggagt ctttctcttg tatggcattt atttctcaca gaatttagaa attgtgacta
     8161 tttttgtctt atttgtgatt ggtgctgcga cttacggctc tttaacagcg gataaaaaaa
     8221 ttattttgaa acaaggtgga tcagctattt tggccttgat tagtatttta ctctttaaat
     8281 acacttgaag gtcgattcta atctcgctaa tcctttttaa tccagaataa gggaaatatg
     8341 ttatacttgt ttttaagaaa aaagtctcat tgaattggtt ttgaggagtt agaaatgaaa
     8401 gtattagtga caggttttga gccctttgga ggggaaaagg gcaatccagc tttggaggcc
     8461 attaaaggtt taccagctga aatccatggt gctgaggtcc gttggctaga ggtgccgaca
     8521 gtttttcaca aatctgctca agtattggaa gaagagatga atcgttatca acctgacttt
     8581 gtcctttgta ttgggcaagc tggtggaaga actagtttga cacctgaatg agtggccatt
     8641 aatcaagacg atgcacgcat ttctgataac gaagataatc aaccgattga ccgtcccatt
     8701 cgcccagatg gtgcttcggc ctactttagt agtttgccga ttaaagcgat ggttcaagct
     8761 ataaaaaaga aggattaccg gcctctgttt ccaatacggc agggactttt gtctgcagcc
     8821 atttgatgta tcaggctctc tatttggtag aaaagaaatt tccatatgtt aaggcaggtt
     8881 ttatgcatat tccttatatg atggaacagg tggtgaacag accgactact ccaactatga
     8941 gtttagtgga tattcggcga gggatagaag cagcaatcgg cgctatgata gaacatggag
     9001 attaggaact caagttggta ggcggagaaa ctcattgata gaaaaaagct tgaggggaaa
     9061 aaccttcaag cttttggacg ttttcgagcc aatactgctt ggtaaaacat aattttagtg
     9121 cattggatat aaggtaggag tgaaaaacta gcaatgccaa aggtaatcca attgaggaag
     9181 taccaaggaa gaagctgtaa atctaggaca aagtgctgga acttgtagcc cttcataaag
     9241 gaacggctag tttttaggat tcgtcttggt gggacctgtc ctaggtctag actataacag
     9301 agaagaaatt ccacctgtga ataggcataa tactgtggaa tatagaggat attccctaca
     9361 atgatcaaga tgagacttgc aagaaagtag agtccaaaga ccatgaggaa acgctcggtt
     9421 tcaactgatg agagatctag atttggaaac tcaggatgta gggtgacgaa ttttttggct
     9481 aaaaagctac tataaaagag gaggtaaatc ccaagtaaat tagggatact ccataaaaag
     9541 agatagaaac gtttgagaag tagggtcaaa aaggtttgag aaaagcgctc ctcatcaaag
     9601 agagctaggc tgttttttac agatggctcc gttttagaat ctttcatgag tgtcagtgtt
     9661 gcatagacgg aactggtcaa aagaatagtc ccgataaagg agactagtag aggaaagagg
     9721 taggcttgaa gtatttggcc aagtatgctg aaaaatggct gttctaaaac agtcccgtgg
     9781 atccgagata agggattaag aaaactagat aagatgacca gcatactggg aaggatatag
     9841 aggagaaaga gacggggggt gtcagcctga aaatgttttg actcctgacg aattgttttt
     9901 aaatcaattt ttggatagtt cattctctta ttataccata gttcttatac atagttcgtg
     9961 acagttccta ctttttttga taaaatcata cagtgtgtcc ttgggcacac tgtatgaact
    10021 gggactgtct ttcccagctt cggaggtaaa aaatgtcaga ttcaccaatc aaatatcgtt
    10081 tgattaagaa agaaaaacac acaggagctc gtctaggaga aatcatcact ccccacggta
    10141 cctttccgac acctatgttt atgccagttg ggacacaagc cactgtcaaa actcagtcac
    10201 ctgaagaatt gaaggagatg ggttcgggaa ttatcctatc aaacacctat catctctggc
    10261 ttcgccctgg agatgaactc attgcacgcg ctggtggtct ccacaagttc atgaattggg
    10321 accagcctat cttgacagat agtggtggtt ttcaggttta ttctttagca gatagccgta
    10381 atatcacaga agaaggagta acctttaaaa atcatctaaa tggttctaag atgttcctat
    10441 ccccagaaaa agccatctct attcagaata atctgggttc agacatcatg atgtcctttg
    10501 atgaatgtcc tcagttttat caaccttatg actacgttaa gaaatcgatc gagcgtacca
    10561 gccgttgggc tgagcgtggt ttgaaggctc accgtcgtcc acatgaccaa ggtttgtttg
    10621 gaattgtgca aggtgcagga tttgaagacc ttcgccgcca atcagctcat gatcttgtca
    10681 gcatggattt ctcaggctac tctatcggtg gtttggcagt gggagaaacc catgaagaga
    10741 tgaatgcggt cttggacttt acaactcaac tgctacctga aaataaacct cgttatctga
    10801 tgggtgtggg agcgccagat agcttgatcg atggggtcat tcgtggggtg gatatgtttg
    10861 actgtgtctt accgactcga attgctcgta acgggacttg tatgaccagt caaggacgtt
    10921 tggttgtgaa aaatgcccag tttgctgagg actttacgcc actggatcct gagtgtgact
    10981 gctacacatg taataactat acacgcgctt accttcgtca cctgctcaag gctgatgaaa
    11041 cctttggtat ccgcttgact agctaccaca atctttactt cttgcttaac ctgatgaagc
    11101 aagtgcgaca agccatcatg gatgacaatc tcttggaatt ccgtgagtat tttgtggaaa
    11161 aatatggcta taataagtca ggacgtaatt tctaaaatgg aattgatata aaaaaatcct
    11221 aagttttctc ttaggatttt tcttcttttt ttgatagaat aaagtgtaca atgaaaggaa
    11281 gaataaactc gtatgcgcat taaatggttt tccttgatta ggattatagg tttacttttg
    11341 gtactcttgt atcacttctt tcagacgatc tttcctggag gatttttcgg ggtagatgtc
    11401 tttttcacat tttcaggttt cctgattaca gctctactca ttgaagaatt ttctaagaac
    11461 aatgagattg atttgatagg attttttaga agacgctttt atcggattgt gccacctgtg
    11521 gttttgatgg tcttggtgac catgcctttt actttcttgg ttcgccaaga ctatgttgct
    11581 ggaattggtg gccagattgc gggcgtctta ggctttatga ccaacttcta tgaactccta
    11641 acaggtggga gttatgaatc tcagttcatt cctcatttgt ttgttcataa ttggagtttg
    11701 gcagttgagg ttcactacta tattctttgg ggattggcag tttggttctt atccaaacaa
    11761 gctaaatcaa atggtcagtt gaaggggatg gtctttctct tatctgctgc tgccttcttg
    11821 atcagtttct tctccatgtt tattggtagt tttctagtga cctcttattc ctctgtttat
    11881 ttctccagtt taactcatgt ctatccattc tttttgggaa gtatgttagc aactattgta
    11941 ggcgttcgtc agacgacttc cctcgtcaag cagttggata aaatctggga tttacgaaag
    12001 actttggtag tttttggagg aggctttggt ttcctagttc ttttgacttt ctttgtcaaa
    12061 ttcacttatc tttttgccta tcttatcggc ttcttgcttg ccagtcttgc agctcttgcc
    12121 atgattctgg cggcgcgtgt cttacatgaa aagacacatc atatacagga gccgaagatt
    12181 atcagctttt tagcggatac tagctatgcg gtttatcttt tccattggcc ttcttatatc
    12241 attttctcac agttgacatc aaatcttctt gctgtattac tgactctgat ttgttcttat
    12301 ggctttgcca gtctgtcatt ctatgtattg gaaccttgga ttgcaggcaa gaacacacct
    12361 attttacaaa ctcttcgtcc cctgccttat attcacgcaa ttcttgcagc aggtacagga
    12421 atcttgacct tcattgtctg catggtgacc ttgttggcac cacaagtggg agcgtttgag
    12481 acagacttga ctgttaatgg cttgaagcaa gctgcaacaa atattggcca gaccaaggtg
    12541 atggcagaac gggcagatgc aaacagtttg ggaattgctg atggcactat gttaattggt
    12601 gactcagtgg ctttaagggc aaatacagca ctacagacag ctcttcctgg agcacagatt
    12661 aacgcgcagg tcagcgtaac aaccaagacc gcaaatgaaa tcatgctaaa taatagccag
    12721 aataaatttt tacctaagac ggtggtcatt gcgactgggg taaataatcc tgagaattac
    12781 aaggatgact gggacagtat cgtgaaaaat cttcctaagg gacaccgtat gattttggtg
    12841 actccttatg agggagataa gacaaaagag acctatgcca tcgttgagaa ggctgctgcc
    12901 tatatgagag aattggcaga gaagacacct tacattacga tagcagattg gaatcaagtt
    12961 gcgaaagagc atccagaaat ttgggcagga acagaccagg ttcatttcgg gagtgagagt
    13021 agcactatcg aagcaggagc aaaattgtat gcagatacga ttgccacagc tttgcagaca
    13081 gctcaagaca agccggttaa atcaaaataa cttgtattaa aaaagagaag agttggagaa
    13141 atccagctct tttaaaatat ctccaaaaaa taggtctata ccatttacaa ataaaaaaga
    13201 aaggtttata atgtaattga cataataaat tgtagaatca atcttttaag gaggttaaca
    13261 ttatgcctaa ctatattaaa gcggatcagt ttttctaccc acacggagtt cgtcgaggtg
    13321 gttacttgga acttgtggac ggcaagtttg ggaaacatgt agagcagatt cctgaagggg
    13381 ctgaggtgat tgactataca ggttatagca ttgccccagg tcttgtggat actcatattc
    13441 atggatatgc aggtgtagat gtgatggaca acaacattga aggtacattg catactatga
    13501 gtgaaggact tcttagtacc ggtgttacca gtttcttacc gacaacttta acagccactt
    13561 atgagcaatt gcttgcagtc actgaaaatc ttggaaacca ttataaagaa gcaacaggtg
    13621 ctaagattcg tgggatttat tatgaaggtc catatttcac agaaactttt aagggggcac
    13681 aaaatccaac ttatatgaga gacccgggtg ttgaggagtt tcattcttgg caaaaagcgg
    13741 caaatggctt gcttaataaa attgcccttg caccagaacg tgatggggtg gaagactttg
    13801 ttcgtacagt tacgggcgaa ggtgtgacgg ttgctcttgg acattcaaac gcgacttttg
    13861 atgaagccaa aaaagcagtc gatgctggag cgagtgtttg ggtgcatgcc tacaatggaa
    13921 tgcgtgggtt gactcaccgt gaattgggta tggttggagc catgtaccaa ttgccacata
    13981 cctatgcaga gttgatctgt gatggtcacc acgtagatcc aaaggcttgc gaaattctta
    14041 tcaaacaaaa aggaacagaa aacatcgctc ttatcacaga ctgtatgaca gctgggggat
    14101 tggaagacgg agattatatg ttgggagaat tcccagttgt cgttgcaaat ggaactgcac
    14161 gcctcaaatc gacaggtaac ttggcaggtt ctatcctcaa actcaaagat ggtttgaaga
    14221 atgtggtcga atggggaatt gcgaatccgc atgaagcagt catgatggcc agcttcaacc
    14281 cagctaaatc cgttcacatc gatgacgtct gtggccaaat ccgtgaaggc tacgacgctg
    14341 acttcatcgt attagataaa gatttggaat tggtagcaac ctacctagat ggcgtgaaac
    14401 gttatcaagc ataagagaga aggcagaagt tagaaactag cttctgcttt tttatacagg
    14461 gtactttact ggtaaataaa aagttaaaaa aatcacaaaa aaagcttgaa gaaacaaaga
    14521 aaaaggctta caattgaact ataaatatta caaataaaaa aacggaggag tgctttatga
    14581 aagcctatac ttatgttaaa ccaggacttg cttcttttgt tgatgtagac aaaccagtta
    14641 ttcgcaagcc aacagacgct attgtgcgta ttgtaaaaac cactatttgt ggaacagacc
    14701 tccatattat caaaggggat gttcctactt gccaaagtgg taccattctt ggccacgaag
    14761 ggattgggat tgttgaagaa gttggggaag gagtttccaa cttcaaaaaa ggtgacaagg
    14821 tcttgatttc ttgcgtctgt gcctgtggta aatgctacta ctgtaaaaaa ggaatttatg
    14881 cccactgtga agacgaaggg ggctggattt tcggtcactt gattgatggt atgcaggctg
    14941 aatatctacg tgtccctcat gcagataata ctctttacca tactccagaa gacttgtcag
    15001 atgaagcttt ggttatgctg tcagacattc tgcctactgg atatgaaatt ggtgtcttaa
    15061 aagggaaagt agaacctggt tgcagcgtag ccattattgg ttcaggtcca gttggattgg
    15121 ctgctctttt aacagcccaa ttctattcac cagctaaatt gattatggta gacctagacg
    15181 ataaccgctt ggaaactgcc ctatcattcg gtgcgactca taaggttaat tcttcagacc
    15241 ctgaaaaagc cattaaagaa atttatgatt tgacagatgg tcgtggtgtg gatgtcgcta
    15301 tcgaagctgt tggtattcct gaaacatttg atttctgtca aaagattatc ggtgtagacg
    15361 gaacggttgc caactgtggt gtgcatggta aaccagttga attcgattta gataaacttt
    15421 ggattcgcaa catcaatgta acaactggtt tggtatctac aaatacgact ccacaattgt
    15481 tgaaagcact tgaaagtcat aagattgaac cagaaaaatt ggtaactcac tatttcaaac
    15541 tcagtgaaat tgaaaaagcc tatgaagtct tcagtaaggc agcagaccac catgccatta
    15601 aggtcattat cgaaaacgat atctcagaag cctaagtagt aaaaatattt ttgtacataa
    15661 gtaaatagaa attcagtcat ccatcagatg gctggatttt ttatcaaaaa attaagaaat
    15721 gagcatattt ctttccttgt ctggcggaat tggttataat atacggtaca aaggaatgaa
    15781 tgaatatgta tcgtgttata gaaatgtacg gagattttga accgtggtgg ttcttagaag
    15841 gttgggaaga agatattgta gcaagtagaa aatttgacca gtattatgat gctctcaaat
    15901 actacaaaac ttgctggttt agattggaac aagaatcccc tctttataaa agtagaagcg
    15961 acttgatgac cattttttgg gacccggaag accaacgctg gtgtgatgaa tgtgatgagt
    16021 atttacaaca ataccattct ttggctcttt tgcaggatga gcaggttatc ccagacgaaa
    16081 aactacgctc aggctatgaa aaacaaacca gtcaggaaag gaatcgttct tgccgtatga
    16141 aattaaaata gagaaaagta acttttttgg agttgctttt tttatttttc taacgctttg
    16201 cgaatagtat aggtgaggag gtaagtatgg ttcaagaaat tgcacaagaa atcattcgtt
    16261 cagctcggaa aaaagggacg caggatattt attttgtccc taagttagac gcctatgagc
    16321 ttcatatgag ggtaggagac gagcgctgta aaattggtag ctatgatttt gaaaagtttg
    16381 cagccgttat cagtcacttt aagtttgtgg cgggtatgaa tgtgggagaa aaaagacgta
    16441 gtcaactggg ttcctgtgat tatgcctatg accagaagat ggcgtctcta cgtttatcta
    16501 ctgtaggcga ttatcggggg catgagagtt tggttatccg tttgttgcac gatgaggagc
    16561 aggacttgca tttttggttt caggatattg aagaattagg caagcagtac aggcaacggg
    16621 gactctatct ttttgctggt ccggttggga gtggtaagac gaccttgatg catgaattgt
    16681 ccaagtcact ctttaaagga cagcaagtta tgtccatcga agatcctgtc gaaatcaagc
    16741 aggacgacat gcttcagttg cagttgaacg aagcaatcgg cctaacctat gaaaatctaa
    16801 tcaaactttc cttgcgtcat cgaccagatc tcttgattat cggagaaatt cgtgacagcg
    16861 agacggcgcg tgcagtggtc agagctagtt tgacaggtgc gacagtcttt tcaaccattc
    16921 acgccaagag tatccgaggt gtttatgagc gtctgctgga gttgggtgtg agtgaagaag
    16981 aattggcagt tgttctgcaa ggagtctgct accagagatt aatcggggga ggaggaatcg
    17041 ttgactttgc aaacagagat tatcaagaac accaagcagc caagtggaat gagcaaattg
    17101 accagcttct taaagatgga catatcacaa gtcttcaggc tgagacggaa aaaattagct
    17161 acagctaagc aaaaaaatat catcacccta tttaacaatc tcttttctag cggttttcat
    17221 ctggtggaga ctatctcctt tttagatagg agttccttgt tggacaagca gtgtgtgacc
    17281 cagatgcgta caggcttgtc tcaggggaaa tcattctcag aaatgatgga aagtttggga
    17341 tgttcaagta ccattgtcac tcagttatct ctagctgaag ttcatggcaa tcttcacctg
    17401 agtttgggaa agatagaaga atatctagac aatctggcca aggtcaagaa aaagttaatt
    17461 gaagtagcga cctatccttt gattttgctg ggttttcttc tcttaattat gctggggcta
    17521 cggaattacc tgctcccaca actggatagt agcaatattg ccacccgaat tatcggtaat
    17581 ctgccacaaa tttttctagg catggtaggg cttgtttccg tgcttgccct tttagcacta
    17641 actttttata aaagaagttc taagatgagt gtcttttcta tcttagcacg ccttcccttt
    17701 attggaatct ttgtgcagac ctacttgaca gcctattatg cacgtgaatg ggggaatatg
    17761 atttcacagg gaatggagct gacgcagatt tttcaaatga tgcaggaaca aggttcccag
    17821 ctctttaaag aaatcggtca agatctggct caaaccctga aaaatggccg tgaattttct
    17881 cagacgatag gaacctatcc tttctttagg aaggaattga gtctcatcat agagtatggg
    17941 gaagttaagt ccaagctggg tagtgagttg gaaatctatg ctgaaaaaac ttgggaagcc
    18001 ttttttaccc gagtcagccg caccatgaat ttggtgcagc cactggtttt tatctttgtg
    18061 gcactgatta tcgttttact ttatgcggca atgctcatgc ccatgtatca aaatatggag
    18121 gtaaattttt aaaatgaaaa aaatgatgac attcttgaaa aaagctaagg ttaaagcttt
    18181 tacattggtg gagatgttag tagtcttgct gattatcagc gtgcttttct tgctctttgt
    18241 acctaatctg accaagcaaa aagaagcagt caatgacaaa ggaaaagcag ctgttgttaa
    18301 ggtggtggaa agccaggcag aactttatag cttggataaa aatgaagatg ctactctgag
    18361 caagttacaa ggagatgggc gcatcacgga agaacaggtt aaagcttata atgaatacta
    18421 tactaaaaat ggaggggcaa atcgtaaagt caatgattaa ggcctttacc atgctggaaa
    18481 gtctcttggt tttgggactt gtgagtatcc ttgccttggg cttatccagc tctgtccagt
    18541 ccacttttgc agcagtagag gagcagattt tctttatgga gtttgaagaa ctctatcggg
    18601 aaacccaaaa acgcagtgta gccagtcagc aaaagactag tctaaattta gatgggcaga
    18661 cgcttagcaa tggcagtcaa aagttgacag ttcctaaagg aattcaggca ccatcaggcc
    18721 aaagtattac atttgaccga gctgggggca attcgtccct ggctaaggtt gaatttcaga
    18781 ccagtaaagg agcgattcgc tatcaattat atctaggaaa tggaaaaatt aaacgcatta
    18841 aggaaacaaa aaattagggc agtgatttta ctggaagcag tagtcgctct agctatcttt
    18901 gccagcattg cgaccctcct tttgggacaa attcaaaaaa ataggcaaga ggaagcaaaa
    18961 atcttgcaaa aggaagaagt cttgagggta gctaagatgg ccctgcagac ggggcaaaat
    19021 caggtaagca tcaacggagt tgagattcag gtattttcta gtgaaaaagg attggaggtc
    19081 taccatggtt cagaacagtt gttggcaatc aaagagccat aaggtcaagg cttttacctt
    19141 gttagaatcc ctgcttgccc tcattgtcat cagtggggga ttactccttt ttcaaactat
    19201 gagtcagctc ctcatttcag aagttcgcta ccagcaacaa agcgagcaaa aggagtggct
    19261 cttgtttgtg gaccaacttg aggcagaatt agaccgttcg cagttcgaaa aagtagaagg
    19321 caatcgccta tacatgaagc aagatggcaa ggacatcgcc atcggtaagt caaagtcaga
    19381 cgattttcgt aaaaccgatg ctagtggacg ggggtatcag cctatggttt atggcctcaa
    19441 atcagctcag attacagagg acaatcaact ggttcatttt cgtttccagt ttcaaaaagg
    19501 cttagaaagg gagttcatct atcgtgtgga aaaagaagaa agttaaggca ggtgttctcc
    19561 tctacgcagt caccatagca gccatcttta gtcttttgtt gcaattttat ttgaaccgac
    19621 aaatcgccca ctatcaagac tatgctttaa ataaagaaaa attggttgct tttgccatgg
    19681 ctaaacgaac caaggataaa gccgagcaag aaagtgggga acaggttttt aatctaggtc
    19741 aggtaagcta tcaaaacaag aaaacaagct tggtgacgac tgttcgtacg tctaagagcc
    19801 aatatgagtt tctgtttcct tcagtcaaaa tcaaagaaga gaaaagagat aaaaaggaag
    19861 aggtagcgac cgattcaagc gaaaaagcgg agaaaaaaat cagaagagaa gcctgaaaag
    19921 aaagagaatt cctagtcaat tcaactataa tacgttgaat ccagaatagt ccactgtagt
    19981 ttctagaaaa ttgctggaaa tggatgttaa gctccaattc atttgtttat atcttatttc
    20041 agtttactat actttgtgct aaattaaaga tatgaaacat gattttaacc acaaagcaga
    20101 aactttcgat tcccctaaaa atatcttcct cgcaaacttg gtatgtcaag cagccgagaa
    20161 acagattgat cttctatcag acaaagaaat tttagatttc ggtggtgaca cgggtctatt
    20221 agccttgccc ctagccccta gccaagcagg ctaagtcagt cactcttgta gacatttctg
    20281 agaaaatgtt ggagcaagct cgtttgaaag tggagcagca agcaatcaag aatatccagt
    20341 ttttggagca agatttaccg aaaaatccct tggagaaaga gtttgatttc cttgctgtta
    20401 gtcgggttct tcatcatatg cctgatttgg atgcggctct ctcactgttt catcaacatt
    20461 tgaaggaaga tgggaaactc atcattgctg attttaccaa gacagaagct aatcatcatg
    20521 gatttgattt agctgaactg gaaaacaagc taattgagca tggtttttca tctgtgcata
    20581 gtcagattct ctatagcgct gaagacctgt ttcaaggaaa tcactcagaa ttctttttaa
    20641 tagtagccca aaaatcactc gcctagtcag ggagtgattt ttctataagg atggaaaaaa
    20701 gaagggaaat ttgataagat aggaatatgg attttgaaaa aattgaacaa gcttatatct
    20761 atttactaga gaatgtccaa gtcatccaaa gtgatttggc gaccaacttt tatgacgcct
    20821 tggtggagca aaacagcatc tatctggatg gtgagactga actagaacag gtcaaagaca
    20881 acaatcaggc ccttaagcgt ttagcactac gcaaagaaga atggctcaag acctaccagt
    20941 ttctcttgat gaaggctggg caaacagaac ccttgcaggt caatcaccag tttacaccgg
    21001 atgctattgc tttacttttg gtgtttattg tggaagagct gtttaaagag gaggaaatta
    21061 ctatcctcga aatgggttct gggatgggaa ttttgggcgc tactttcttg acctcgctta
    21121 ctaaaaaggt ggattatttg ggaatggaag tggatgattt gctgattgat ttggcagcta
    21181 gtatggcaga tgtaattggt ttgcaggctg gctttgtcca aggagatgcc gttcgtccac
    21241 aaatgctcaa agaaagtgat gtggtcatca gcgacttgcc tgttggctat tatcctgatg
    21301 atgccgttgc gtcgcgccat caagttgctt ctagccaaga acatacttac gcccatcact
    21361 tgctcatgga acaagggctt aagtacctca agtcagacgg atacgctatt tttctagctc
    21421 cgagtgattt gttgaccagt cctcaaagtg atttgttaaa agaatggctg aaagaagagg
    21481 cgagtctggt tgctatgatt agtctgcctg aaaatctctt tgctaatgct aaacaatcta
    21541 agactatttt tatcctacag aagaaaagtg aaatagcagt ggagcctttt gtttatccac
    21601 ttgctagctt gcaagatgca agtgttttaa tgaaatttaa agaaaatttt caaaaatgga
    21661 ctcaaggtac tgaaatataa aatagatttt gttataatag ttgaaaacgc ttaaaaaggg
    21721 gtatcatgtt atgacaaaaa caattgcaat caatgcagga agttcaagtt tgaaatggca
    21781 attatactta atgccagagg aaaaagtgtt agccaaaggt ttgattgaac gtatcggttt
    21841 gaaagattca atttcaactg taaaatttga cggccgttct gaacaacaaa ttttggatat
    21901 cgaaaatcat acacaagctg ttaaaatttt attggatgac ttgattcgtt tcgatattat
    21961 caaggcttat gacgagatta caggtgttgg ccaccgtgtt gtagcaggtg gagaatattt
    22021 caaagaatca acagttgttg agggagatgt tttagaaaaa gttgaagagt tgagtttgtt
    22081 ggctcctcta cacaacccgg ccaatgcagc aggtgttcgt gctttcaagg aattgttgcc
    22141 agacattacc agtgtagttg tttttgatac ttccttccac acaagtatgc cagagaaagc
    22201 ttatcgctac cctctaccaa caaaatatta cacagaaaac aaggttcgta aatacggtgc
    22261 tcatggtaca agtcaccagt ttgtagcagg agaagctgca aaactcttgg gacgtccatt
    22321 agaagacttg aagttaatta cctgtcatat tggtaacgga ggctcaatta cagctgtgaa
    22381 agccggcaaa tctgtagaca cttctatggg gttcactcct cttggtggta ttatgatggg
    22441 aacgcgtaca ggggatattg atccagctat cattccttat ttaatgcaat atacagagga
    22501 ttttaacaca ccagaagata tcagtcgtgt tcttaaccgt gaatcaggtc ttttgggagt
    22561 ttctgctaat tctagcgata tgcgcgatat agaagcagct gtagcagaag ggaatcacga
    22621 ggctagcttg gcttatgaaa tgtatgttga ccgtatccaa aaacatatcg gtcagtacct
    22681 tgcagtgcta aatggaacag atgccattgt tttcacagca ggtgtcggtg aaaatgcaga
    22741 gaatttccgt cgtgatgtaa tctcagggat ttcgtggttt ggttgtgatg ttgatgatga
    22801 aaagaatgtc tttggcgtta caggagacat ctcaacagag gcagctaaaa tccgtgtctt
    22861 ggttattcca acagatgaag aattagtcat tgcccgtgac gttgaacgct tgaaaaaata
    22921 agtgaaacta aaaaaatatt caatacaagg agttgggaaa gttatttttc cagcttcttt
    22981 ttctgatgaa attgtccaaa accttgctat gattggcttt tttgaaaaat atggtataat
    23041 agtagtaatt taatagatgg agttgagttt tgaagaaaaa ctttcgtgta aaaagagaga
    23101 aagattttaa ggcgattttc aaggagggga caagttttgc taatcgcaaa tttgtggtct
    23161 accaattaga aaaccagaaa aaccattttc gagtaggtct atcagttagc aaaaaactgg
    23221 ggaatgccgt cactagaaat caaattaagc gacggattcg gcatattatc cagaatgcaa
    23281 aagggagtct ggtagaagat gtcgactttg ttgtcattgc tcgaaaagga gtcgaaacct
    23341 tgggatacgc agagatggag aaaaatctac tccatgtatt aaaattatca aagatttacc
    23401 gggaaggaaa tgggagtgaa aaagaaacta aagttgacta gtttgctagg actgtctctg
    23461 ttaatcatga cagcctgtgc gactaatggg gtaactagcg atattacagc cgaatcggct
    23521 gatttttgga gtaaattggt ttacttcttt gcgaaaatca ttcgcttttt atcgtttgat
    23581 attagtatcg gagtggggat tattctcttt acggtcttga ttcgtacagt cctcttgcca
    23641 gtctttcagg tgcaaatggt ggcttctagg aaaatgcagg aagctcagcc acgcattaag
    23701 gcgcttcgag aacaatatcc aggtcgagat atggaaagca gaaccaaact agagcaggaa
    23761 atgcgtaaag tatttaaaga aatgggtgtc agacagtcag attctctttg gccgattttg
    23821 attcagatgc cggttatttt ggccttgttc caagccctat caagagttga ctttttaaag
    23881 acaggtcatt tcttatggat taaccttggt agtgtggata caacccttgt tcttccgatt
    23941 ttagcagcag tattcacctt tttaagtact tggttgtcca acaaagcttt gtctgagcga
    24001 aatggcgcta cgactgcgat gatgtatggg attccagtct tgatttttat ctttgcagtt
    24061 tatgcgccag gtggagtcgc cctatactgg acagtgtcta atgcttatca agtcttgcaa
    24121 acctatttct tgaataatcc attcaagatt atcgcagagc gcgaggccgt agtacaggca
    24181 caaaaagatt tggaaaatag aaaaagaaaa gccaagaaaa aggctcagaa aacgaaataa
    24241 ataaggagga atctggtaat ggtagtattt acaggttcaa ctgttgaaga agcaatccag
    24301 aaaggattga aagaattaga tattccaaga atgaaggctc atatcaaagt catttctagg
    24361 gagaaaaaag gctttcttgg tctatttggt aaaaaaccag cccaagtgga tattgaagcg
    24421 attagtgaaa cgactgttgt taaagcaaat caacaggtag taaaaggcgt tccgaaaaaa
    24481 atcaatgatt tgaacgagcc tgtgaagacg gttagtgaag aaaccgttga ccttggtcat
    24541 gtggttgatg ctattaaaaa aatagaggaa gaaggtcaag gtatttctga tgaagtcaag
    24601 gctgaaatct taaaacatga aagacatgcc agcactatct tagaagaaac tggtcacatt
    24661 gagattttaa atgaacttca aatcgaggaa gcgatgaggg aagaagcagg cgctgatgac
    24721 cttgaaactg agcaagatca aactgaaaat caagacttga aagagatggg cttgaaggtc
    24781 gagcaaagtt atgatattgc ccaggtggct acggatgtga ctgcctatgt tcaagcgatt
    24841 gtggatgaca tggatgttga agctacactt tcaaatgatt ataaccgtcg tagcatcaat
    24901 ctacaaattg acaccaacga accaggtcgt attatcggct accatggtaa agtcttgaag
    24961 gccttgcaac tgttggctca aaattatctt tacaaccgct attccagaac cttctacgtt
    25021 acaatcaatg tcaatgatta tgtcgaacac cgtgcagaag tcttgcagac ctatgcgcaa
    25081 aaattggcga ctcgtgtttt ggaagaaggg cgcagtcata aaacagatcc aatgtcaaat
    25141 agcgaacgca agattatcca tcgtattatt tcacgtatgg atggcgtgac tagttactct
    25201 gaaggtgatg agccaaatcg ctatgttgtt gtagatacag aataagtaaa atcaggttta
    25261 tcctgatttt ttgctagtta gaggaggtta aactgatgtt gaataagata agagactatt
    25321 tagactttgc tggtttgcag taccgtaatc ctgataaagc gggagcagag cgagagaaga
    25381 tgctggcatt ccgccacaaa ggacaagagg cccgaaaggc ttttacagaa ctggccaaag
    25441 cctttcaagc aagccatcca gaatggcaac tccaacagac tagccagtgg atgaatcagg
    25501 cccagcgttt gagaccacat ttttgggttt atctacagag agacggacaa gtgacagaac
    25561 ctatgatggc cttacgtttg tatgggacat ctactgactt tggaatttct ttggaagtca
    25621 gtttcatcga acgtaagaag gatgagcaaa cactgggcaa gcaggccaaa gttttagaaa
    25681 ttccaaccgt taaagggatt tattatctaa cctactctga tggtcaaagt caacggtggg
    25741 aggcgaatga agaaaagcgt cgtactttac gcgagaaggt gagaagtcaa gaagttcgaa
    25801 aagttttagt gaaggtagat gttcctatga cagaaaattc gtctgaagaa gaaatcgtag
    25861 aaggcttatt gaagtcttat tctaaaattc ttccctatta tctagctacg agaaaataag
    25921 ataatttgta aaacatcata aatcatacag tccaagagtg aacagtccgc tgtgtaattc
    25981 ttggtctttt tgtttgcgct ttcgcattat ataataaact tacaaaaaca attcaaaagg
    26041 agaacaatta tggaagtcgt ttcaagtgtt ctaaattggt tttctagcaa tattttgcag
    26101 aatcccgcat ttttcgtagg tttattggtg ttgataggat atgcactttt gaaaaaacct
    26161 gcccatgacg ttttttcagg gtttgttaaa gcaacagtag ggtatatgtt gcttaacgtg
    26221 ggtgctggtg gtttggttac aacctttcgt ccaatcttag cggctcttaa ctacaaattc
    26281 caaattggtg cagcggttat cgacccttac tttggacttg ctgcagcaaa caacaaaatt
    26341 gcagcagagt ttccagattt tgttggaact gcgactacag ctctattgat tggtttcgga
    26401 ataaatatct tgctcgtagc tcttcgaaag attacgaagg taagaaccct ctttattact
    26461 ggtcacatca tggtacaaca agctgcaaca gtatctctta tggttctatt cttagtacca
    26521 caattgcgca atgcttacgg tacagcagcg attggtatca tctgtggact ttactgggca
    26581 gttagttcaa atatgactgt tgaggcaact caacgcttga ctggtggtgg cggatttgcg
    26641 attggtcacc aacagcaatt tgcaatctgg tttgtagata aagtagcagg acgctttggt
    26701 aagaaagaag aaagtttaga caatcttaaa ttacctaagt tcctctcaat cttccacgat
    26761 acagttgttg catctgctac cttgatgctc gtattcttcg gagccattct tttaatcttg
    26821 ggtccagaca ttatgtctaa taaagaagtc atcacttcag gaactctatt caatcctgct
    26881 aaacaagatt tctttatgta cattatccaa acagccttta ccttctcagt ttacttgttc
    26941 gttttgatgc aaggtgtccg aatgttcgta tctgagttga caaacgcctt ccaaggtatt
    27001 tcaaacaaat tgttgccagg ttcattccca gcggttgacg ttgcagcttc ttatggattt
    27061 ggttctccaa atgctgtcct gtcaggattt atctttggtt tgattggtca attgattaca
    27121 attgttttgc tcatcgtctt taaaaatccg attcttatta ttacaggatt tgtaccagtg
    27181 ttctttgaca atgcagccat tgcggtctac gctgataaac gcggcggatg gaaagcggct
    27241 gttatccttt cctttatatc aggtgtcctt caagttgctc taggagctct ttgtgtggcc
    27301 cttctcgatt tggcatctta tggtggctac catggaaata tcgactttga attcccatgg
    27361 cttggatttg gatatatctt caaatacctt ggtattgttg gttatgtact tgtgtgtctc
    27421 ttcttgcttg ttattcctca acttcaattt gccaaagcaa aagataaaga gaaatattac
    27481 aacggtgaag ttcaagaaga agcttagtat ctagaaaagg agaaataaaa tggttaaagt
    27541 attagcagcg tgcggaaatg gaatgggttc atcaatggtt atcaagatga aggttgaaaa
    27601 tgctctccgt aagcttaatc aaacagattt tacagtcaat tcatgcagtg tcggtgaagc
    27661 taaaggttta gcagtaggat atgacatcgt aatcgcttct cttcatttga ttcaagaatt
    27721 ggaagggcga actaatggga agttaattgg gcttgataac ttgatggatg ataaagaaat
    27781 caccgaaaaa ctcagtcaag cactacagta aaaggttgga gggggctgga cagaaactga
    27841 gagttatcgt ttctgtcctt ctccctcttt aaataaagga ggcagatatg aatttaaaac
    27901 aagctttaat tgacaacgac tcgatccgac taggtttaga ggctaacgat tggaaagaag
    27961 cagtcaaggt agcagtagat cccttgattg aaagtggggc aattttgcca gagtattacg
    28021 atgctatcat tgaatcgact gaagagtatg ggccttacta tatcttgatg ccaggtatgg
    28081 ctatgcccca cgctagacct gaagctggtg tgcaaagtga tgccttttca ttaattacct
    28141 tacaaaatcc tgttgtattt tcagatggga aagaggtatc tgttttgttg gcactagcag
    28201 caacaagttc aaaaattcac acaagtgtag ccattccaca aattattgcc ctgtttgaat
    28261 tagaagattc tattgcacgt ttacaggctt gccagactaa agaagatgtc ttggctatga
    28321 ttgaagaatc taaggatagc ccttatctcg aaggattgga tttggaaagt tagaaagagg
    28381 aataaagaaa tgacaaaaag aatacctaat ttacaagttg cattagacca ttcagacttg
    28441 caaggagcga ttaaagcagc tgtttctgtt ggtcaggaag tagatattat cgaagctgga
    28501 actgtttgct tgcttcaagt tggaagtgaa ctggctgaag tcttgcgtag ccttttccca
    28561 gataagatta ttgtggcaga cacaaaatgt gctgatgctg gtggaacagt tgctaaaaat
    28621 aatgcggttc gtggagcaga ctggatgact tgtatctgtt gtgcaaccat ccctactatg
    28681 gaagcagctc taaaggctat caagactgaa cgaggagaac gaggcgaaat ccagatcgag
    28741 ctttatggcg attggacttt tgaacaagct cagctttggc tagatgcagg tatctcacaa
    28801 gctatttatc accaatctcg tgatgctctt cttgctggtg aaacttgggg tgaaaaagac
    28861 cttaataagg ttaaaaaact cattgacatg ggcttccgtg tatctgtaac aggtggtcta
    28921 gatgtagata ctctcaaact ctttgaaggt gttgatgtct ttacctttat cgcaggtcgt
    28981 ggaattacag aggctgcgga tccagcagga gcagcgcgtg ccttcaagga tgaaatcaaa
    29041 cgaatttggg ggtaaatcat ggtacgtcca attggaattt atgaaaaggc aaccccaaca
    29101 cactttactt ggctagaacg tttaaatttt gccaaggagt taggctttga ttttgtcgag
    29161 atgtctattg acgaacgtga cgagcgttta gcaagacttg actggagtaa ggaagaacgc
    29221 ttggaagttg tcaaagcaat ctatgaaact ggtgttcgta ttccttctat ctgtttttca
    29281 ggccatcgtc gctacccatt gggttcaaaa gatccagttc tagaggaaaa atctctagaa
    29341 ctcatgaaaa aatgtatcga attagctcaa gacttgggag ttcgtacgat tcaattagct
    29401 ggttacgatg tttactatga ggaaaagtca ccccagacac gccaacgttt tatcaaaaat
    29461 ttgagaaaag cctgtgactg ggctgaagaa gctcaggtgg tacttgctat tgaaattatg
    29521 gatgatcctt tcatcaatag catcgaaaaa tatttggcta tagaaaaaga gattgactct
    29581 cccttcctct ttgtatatcc agatattggt aatgtgtctg catggcataa tgatatctat
    29641 agtgagtttt atcttggtca tcatgccatc gcagctctcc atctcaagga tacttatgca
    29701 gtgacagaaa gttcaaaggg ccagttccga gatgtacctt tcgggcaagg ttgtgtcaaa
    29761 tgggaagaag ctttcgatat tttaaaggaa accaattata atggaccttt cctaatcgaa
    29821 atgtggtctg aaaattgtga aacagtagaa gaaacacgcg cagccattca agaggcgcaa
    29881 gcttttctct atccactcat taagaaagca ggtttgatgt aagatgaatc aagtaatcaa
    29941 tgctatgcgt aaacgagtct gtgatgccaa tcaatcattg ccaaaacatg gacttgtcaa
    30001 atttacctgg gggaatgtat ctgaagtcaa tcgcgaactc ggtgtcattg ttatcaaacc
    30061 atcaggcgtg gattatgacg aattgacacc tgaaaacatg gtagtgactg atctagatgg
    30121 taagatccta gaaggggatt taagaccatc ttccgacctc ccaactcatg tgcaattata
    30181 tagggcttgg tcagaaattg gtagtgtggt tcacacccat tcgacagaag ctgttggttg
    30241 ggctcaggca ggtcgtgata ttcctttcta cggaacaacc catgcagatt atttctacgg
    30301 ttcaatccct tgcgcccgta gtttgaccaa ggacgaagta gaagtggcct atgaaaaaga
    30361 tactggcctg gttatcgtag aagagtttga acatcgcgga cttaacccgg ttgaagtacc
    30421 aggaattgtt gtacgcaatc acggtccatt cacctggggc aaaaatccag agaatgctgt
    30481 ttatcactct gtcgtactag aggaagtatc aaagatgaat cgctttacag aacaaatcaa
    30541 tccaagagtt ggacctgctc cccagtacat actagaaaaa cactaccaac gtaaacatgg
    30601 accaaatgct tattatggtc aaaagtaaga acgatgaagg aggagaaaaa gataaattta
    30661 gctcctcttt ttacatttga tttttattga gagtaaagtt ggagttgaag taattttaaa
    30721 agatttttta gaaatagcgc ttgatatata tatggtaaaa taaaaagaat tgctgtgata
    30781 tcaatagatt tgggggattt tttaatatgg tactggataa ggcaagttgt gatttgcttc
    30841 aatatttgat ggatcaagaa acgtccaaaa cgattatggc gatttcgaaa gatttgaaag
    30901 agtcaagaag gaaaatttat tatcacattg acaaaatcaa tgctgctctg ggtgacgagg
    30961 cgcttcacat cattagtatt ccacgaattg gtattcactt aacggaagag cagagagatg
    31021 cttgttgtaa actattatcg gaagtagatt cgtacgatta tatcatgagt gcgcatgaac
    31081 gtatgatgat aatgttacta tggataggta tttctaaaga acgtattacg attgaaaaat
    31141 tgatagagtt aacagaggta tctaggaata ctgttctcaa tgatttgaat agtattcgtt
    31201 atcaactaac tttggaacaa tatcaggtga tcttgcaagt gagcaagtca cagggatacc
    31261 accttcatgc ccaccctctt aataaaattc agtatcttca atcgcttcta tatcatattt
    31321 ttatggaaga aaatgccact tttgtatcta ttttagaaga taagatgaaa gagaggttag
    31381 atgatgagtg tttgctttct gttgaaatga accagttttt taaggaacag gttcctttag
    31441 ttgaacaaga tttagggaag aaaataaacc atcatgaaat aacttttatg ttgcaggttc
    31501 taccttattt gctgttaagc tgtcataatg ttgaacagta tcaagaaaga catcaggata
    31561 tagagaaaga atttgctttg ataagaaaaa gaatagagta tcaggtgtct aagaagttag
    31621 gagaacggtt gtttcaaaag tttgaaattt ctttgtcagg acttgaagtt tctcttgtag
    31681 ctgttctcct cctctcctat cgtaaagatt tggatattca tgcagaaagt gatgattttc
    31741 ggcaattaaa acttgcttta gaagaattta tctggtattt tgaatcacaa atccgaatgg
    31801 agattgagaa caaggatgat ttgttacgaa atttgatgat ccactgtaaa gccttgttat
    31861 ttagaaagac ttacggtatt ttttctaaaa atcctctaac aaaacaaatt cgatccaagt
    31921 atggagaatt atttttagtc actagaaaat ctgcggaaat tttagaagga gcatggttta
    31981 ttcggctaac agacgatgat attgcctatt tgacgatcca tattggagga tttttaaagt
    32041 atacaccatc gtctcaaaaa aatatgaaaa aagtttatct cgtttgtgat gaaggtgttg
    32101 cggtttcgag acttttgctg aaacaatgca aactttattt tccaaatgag caaattgaca
    32161 ctgtatttac aacagaacaa tttaagagtg tggaagatat tgcacaagtt gatgtagtga
    32221 ttactactaa tgatgatttg gatagcagat ttccgatttt aagggttaat cctatccttg
    32281 aagcagaaga tattttgaaa atgctagact atcttaaaca caatatattt cgtaatgaga
    32341 gcaaaagttt cagtgaaaat ctttctagtc ttatttcgtc ttatattgta gatagcaagt
    32401 tggctagtaa gttccaagaa gaggttcaaa cacttataaa tcaagaaata gtagttcaag
    32461 cttttttgga agatatttga aggacagtcc aatgatgaac acaaacctgt gtttttcttg
    32521 gtctttttta gtgttttgaa gggtggtata ctaatctcaa agataacaat tatatccaaa
    32581 ggaggcaaca tatgccaaac gtcaaagaaa ttacaagaga gtcatggatt ttagccactt
    32641 tcccagagtg gggaacatgg ttgaacgaag aaatcgaaga agaagtcgta cctgaaggca
    32701 actttgccat gtggtggcta ggcaactgtg gtacttggat taagacacca gctggtgcta
    32761 acgttgtcat ggacctttgg tcaaaccgtg gaaaatcaac caaaaaagtg aaagatatgg
    32821 ttcgtgggca ccaaatggca aatatggcag gtgttcgtaa gttgcaacca aacttgcgtg
    32881 ttcagccaat ggttatcgat ccatttgcta tcaacgaact agactattac ttagtttcac
    32941 acttccacag tgatcatatc gacccataca cagctgcagc aattctcaac aatcctaagt
    33001 tagagcatgt taagtttatc ggtccttacc actgtggacg aatctgggaa ggatggggtg
    33061 ttccaaaaga acgtatcatc gttgttaaac caggtgacac tatcgaatta aaagatatga
    33121 agattcatgc agtagaatca tttgaccgta cttgcttggt aactctccca gtgaacggtg
    33181 ctgatgagac aggcggtgaa cttgctggct tggctgttac agatgaagaa atggctcaaa
    33241 aggctgttaa ctatatcttt gaaacaccag gtggaaccat ctatcatggt gcagattctc
    33301 acttctcaaa ctattttgca aaacatggta aagactttaa aattgatgtt gctttgaata
    33361 actatggtga aaatccggta ggtatccaag acaaaatgac atctatcgac cttcttcgta
    33421 tggcagaaaa tctgcgtacc aaagtcatta tcccagttca ctatgatatc tggtctaact
    33481 tcatggcttc tactaatgag attctagaac tttggaaaat gcgaaaagat cgcttgcaat
    33541 acgatttcca tccatttatc tgggaagttg gcggtaagta cacttatcct caagatcaac
    33601 acttagtaga ataccatcat ccacgtggtt ttgatgattg ttttgaacaa gactctaaca
    33661 ttcaatttaa agctttgcta taatataaaa atattctttg gaggttatcc gtatgttgca
    33721 tgatacggat aattttcata taatagtaaa atagaatgtg tgattcaata atcacctcaa
    33781 atagaaagga aattctatgt caaatctatc tgttaatgca attcgttttc taggtattga
    33841 cgccattaat aaagccaact caggtcatcc aggtgtggtt atgggagcgg ctccgatggc
    33901 ttacagcctc tttacaaaac aacttcatat caatccagct caaccaaact ggattaaccg
    33961 cgaccgcttt attctttcag caggtcatgg ttcaatgctc ctttatgctc ttcttcacct
    34021 ttctggtttt gaagatgtca gcatggatga gattaagagt ttccgtcaat ggggttcaaa
    34081 aacaccaggt cacccagaat ttggtcatac ggcagggatt gatgctacga caggtcctct
    34141 agggcaaggg atttcaactg ctactggttt tgctcaagca gaacgtttct tggcagccaa
    34201 atataaccgt gaaggctaca atatctttga ccactatact tacgttatct gtggagacgg
    34261 agacttgatg gaaggtgtct caagcgaggc agcttcatac gcaggtttgc aaaaacttga
    34321 taagttggtt gttctttatg attcaaatga tatcaacttg gatggtgaga caaaggattc
    34381 ctttacagaa agtgttcgtg accgttacaa tgcctacggt tggcatactg ccttggttga
    34441 aaatggaaca gacttggaag ccatccatgc tgctatcgaa acagcaaaag cttcaggcaa
    34501 gccatctttg attgaagtga agacggttat tggatacggt tctccaaaca aacaaggaac
    34561 taatgctgta cacggcgccc ctcttggagc agatgaaact gcatcaactc gtcaagccct
    34621 cggttgggac tacgaaccat ttgaaattcc agaacaagta tatgctgatt tcaaagaaca
    34681 tgttgcagac cgtggcgcat cagcttatca agcttggact aaattagttg cagattataa
    34741 agaagctcat ccagaactgg ctgcagaagt agaagccatc atcgacggac gtgatccagt
    34801 cgaagtgact ccagcagact tcccagcttt agaaaatggt ttttctcaag caactcgtaa
    34861 ttcaagtcaa gatgccttga atgttgtggc agctaaatta ccaaccttcc taggtggatc
    34921 agctgacctt gctcactcaa acatgactta tatcaagacg gatggacttc aagacgatac
    34981 caatcgcttg aatcgtaaca ttcagtttgg tgttcgtgaa tttgcaatgg gaacaatctt
    35041 gaacgggatg gcccttcatg gtggacttcg tgtatacggt ggaactttct tcgtcttctc
    35101 tgactatgtg aaggcagctg tccgcttgtc agccttacaa ggacttcctg tgacttatgt
    35161 ctttacccat gattcaatcg cagttgggga agatggtccg actcacgaac cagttgagca
    35221 tttagcaggt cttcgtgcta tgccaaatct aaatgttttc cgtccagcag atgcgcgtga
    35281 aacgcaagca gcttggtacc ttgcagtgac aagtgagaaa acaccaactg cccttgtctt
    35341 gacacgtcaa aacttgactg ttgaagatgg aacagacttc gacaaggttg ctaaaggtgc
    35401 ttatgttgta tatgaaaatg cagccgactt tgataccatc ttgattgcga caggttcaga
    35461 ggttaatctt gctgtctcag ctgctaaaga attggctagt caaggcgaaa aaatccgcgt
    35521 agtcagcatg ccatctacag atgtctttga taaacaagat gcagcttaca aggaagagat
    35581 tcttccaaat gcagtccgcc gtcgtgttgc agtcgaaatg ggtgcaagtc aaaactggta
    35641 caaatatgtt ggtctcgatg gtgccgttct aggtattgat accttcggag cctctgcccc
    35701 agcaccaaaa gtattggcag aatatggctt tactgtagaa aatcttgtaa aagttgttcg
    35761 aaacttgaaa taatcctaaa aatcagggcg taagctctgg tttttcttac cagaaaagta
    35821 aggtacaatc ttgtaaaagt agctgaaatt tgatatagta gtcctatgta aaagacaaag
    35881 gagaaaacaa tgaatccaaa tattactttt ttaatcatgc ttgtaggtat gatggccttg
    35941 atgttcttta tgcaacgctc tcaaaagaaa caagctcaaa agcgtatgga aagcttaaat
    36001 aaactacaaa aaggctatga agtgattaca atcggtggac tttatggaac agtcgatgaa
    36061 gtagatacgg agaagggaac aatagttctt gacgtagatg gagtttactt gacttttgaa
    36121 ctagctgcta tcaagacagt attaccgctg aaagaaacag cttcactaga aggcgcaatt
    36181 gaaaaataag acgggatcac gaactcccgt tttctataaa agaaaggaaa tgggatgaaa
    36241 aaattagtct ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg
    36301 aaatcaatga cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc
    36361 aatccgattc ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag
    36421 aacaagacat cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga
    36481 atggacgctt caaatatttc tgacttacgt cagatgtgtc cagtagactg tcaagataag
    36541 atttactcat tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa
    36601 acctatcgac gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt
    36661 taaggtggaa aatcttacga atttttacga aaagtatcgt gtctatctga ctcgtccacg
    36721 tttagagctt ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa
    36781 tattccagga aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct
    36841 tgtccgtggt aaaatgaatg gtcaaggtac cattaccttc caaaatggag accaatatac
    36901 aggtggcttc aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg
    36961 gacctacgaa ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga
    37021 acaagaagtc gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct
    37081 ccttatcaaa ggaggtatta ttagaattac aaggtaagcg cttacctgta aatccctttc
    37141 tttccaaatc cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac
    37201 aaacttcaaa gataaaacct gataagaaaa gaaaatgaga aaagtttcac aagagtttaa
    37261 aaattctttg tgaaatgttt atgaaactgt ttacaaagtt gaaataatac gttataataa
    37321 acttgtgaaa aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct
    37381 gataaaaaaa ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag
    37441 ttggttcaaa aagctctagt tgcccttgaa gaaatgcgta aattggatca agagcaagtt
    37501 gactacatcg ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta
    37561 catgcctttg aagaaacagg acgtggtgta tttgaagata aagcaactaa gaacttgttt
    37621 gcctgtgaac acgtagtaaa caacatgcgt cacactaaga cagttggcgt tatcgaagaa
    37681 gacgatgtaa caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact
    37741 ccaacaacaa acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt
    37801 aacccaatcg tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt
    37861 atcgtccgcg atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact
    37921 caaccatcta tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt
    37981 gcaacaggtg gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg
    38041 gtaggtgccg gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca
    38101 cacgatatcg tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca
    38161 gttatcattg ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact
    38221 tactttgtaa acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg cgtcaaagca
    38281 aacagcaaaa actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact
    38341 tggattgcag aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa
    38401 tgtaaagaag ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca
    38461 gttttgaaat ctgaaagccg tgaagatggt attactaagg ctcgtcaaat ggttgaattt
    38521 aacggtcttg gacactcagc agctatccac acagctgacg aagaattgat taaagaattt
    38581 ggtaaagctg ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc
    38641 ggggacgttt acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc
    38701 aactcagttg gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga
    38761 cggagaaata acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca
    38821 attcaatacc ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc
    38881 atggtagagc ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag
    38941 gttgtttacc aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt
    39001 ggtactgaga ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct
    39061 ccaatggatg ctgccaaagt aatgtggctc ttctacgagc aaccagaagt ggacttccgt
    39121 gaccttgtcc aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt
    39181 aagaagacta aattcatcgc gattccaact acatctggta caggatctga agtaacacca
    39241 tttgccgtta tctctgataa agcaaacaac cgtaaatacc caatcgctga ctactcattg
    39301 acaccaactg tggcaatcgt agatcctgct ttggtattga cagttccagg atttgttgct
    39361 gctgatactg gtatggacgt attgactcac gcgacagaag catacgtatc acaaatggct
    39421 agtgactaca ctgatggttt agcacttcaa gccattaaat tggtctttga aaatctcgaa
    39481 agctcagtta agaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc
    39541 gctggtatgg cctttgccaa tgccttccta ggtatttctc actcaatggc ccataagatt
    39601 ggtgcgcaat tccacacaat ccacggtcgt acaaatgcta tcttgcttcc atacgttatc
    39661 cgttacaacg gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctactaccgt
    39721 gcagatgaaa aataccaaga tatcgcacgc atgcttggac ttccagcttc tactccagaa
    39781 gaaggggttg aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa
    39841 atgaatttta gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta
    39901 gccttcctgg cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac
    39961 catatgcaag aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt
    40021 aaataattgt ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt
    40081 aatttttcta tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt
    40141 ttatgatatt gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa
    40201 tagattgaaa ctagagtagt acacatctgc ttctaaaaca ttgttagaat tcgatttgat
    40261 tgtcctgatc aatttgtcct gttctt
//
LOCUS       DAVKJU010000012        39760 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVKJU010000012 DAVKJU010000000
VERSION     DAVKJU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.012"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            29..373
                     /locus_tag="VW191_000537"
     CDS             29..373
                     /locus_tag="VW191_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623354.1"
                     /translation="MPLPTGKIDLNEYGKHLAEKSAEIYKEKTKQAVDAMERHRQECL
                     ARDAKWQAVIDRSDELLKEKKEREHQQEIERLMEEKRKEVDREVSQRLSMPNEHQKKL
                     NKAMSEMLKDLF"
     gene            571..1869
                     /locus_tag="VW191_000538"
     CDS             571..1869
                     /locus_tag="VW191_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623355.1"
                     /translation="MTLKGMGYLRKKLANYKMGVDTRYNQYAMQHNDIDVGITIPPQI
                     RQQYRAVLGWTAKGVDSLADRLVFREFANDEFGANEIFAQNNPDVFFDSAILSALIGS
                     CCFVYISQGDDDDAPRLQVIEASNATGVLDPITGLLIEGYAVLKRDDNGYAVLEAYFT
                     SDVTWFYPKDGKPFAIENPTGVPLLVPVIHRPDTVRPFGRSRITKAGMYYQRYAKRTL
                     MRSDVTAEFYSFPQKYVLGLSQDAEAIDTWKATVSSLLTFTKDDEGEKPNVGQFTTSS
                     MSPFTEQLRTAAAGFSGEMGLTLDDLGFVSDNPSSVEAIKASHENLRLAGRKAQRSLG
                     SGLLNVAYVATCLCDEFPYLRKQFNKTVVKWEPLFEADANMLTLIGDGVIKLNQAVPG
                     YMDAETIRDLTGIKGSDKPAPVVKEGADGGRYVSESTPEN"
     gene            1878..2135
                     /locus_tag="VW191_000539"
     CDS             1878..2135
                     /locus_tag="VW191_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623356.1"
                     /translation="MKKARLDSEILEELLEKNKVNYLDANQYAIEIGEILAKVLGAYL
                     SNINQEDVERRKQIGIESAEERKRKRDERIAKFGNPRRRLN"
     gene            2172..2564
                     /locus_tag="VW191_000540"
     CDS             2172..2564
                     /locus_tag="VW191_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9623357.1"
                     /translation="MELDKFKTMMNVRKRMTYFLRFQRMAGSENQVRIDEEAWKLVLP
                     DQWHLSGEHEKAIREGLEIFAHDINSIENERARKYFIIHYCYMRKKTMSECVEMAGTS
                     STSYHRYKQISVLNFARIHQNGELEAYK"
     gene            complement(3520..4368)
                     /locus_tag="VW191_000541"
     CDS             complement(3520..4368)
                     /locus_tag="VW191_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU9623358.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLACLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            4520..4798
                     /locus_tag="VW191_000542"
     CDS             4520..4798
                     /locus_tag="VW191_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623359.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            4800..6863
                     /locus_tag="VW191_000543"
     CDS             4800..6863
                     /locus_tag="VW191_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9623360.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(6941..8197)
                     /locus_tag="VW191_000544"
     CDS             complement(6941..8197)
                     /locus_tag="VW191_000544"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU9623361.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            8339..10804
                     /locus_tag="VW191_000545"
     CDS             8339..10804
                     /locus_tag="VW191_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9623362.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLCFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLRENGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLATADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            10869..11780
                     /locus_tag="VW191_000546"
     CDS             10869..11780
                     /locus_tag="VW191_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9623363.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            12010..12708
                     /gene="dapD"
                     /locus_tag="VW191_000547"
     CDS             12010..12708
                     /gene="dapD"
                     /locus_tag="VW191_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEU9623364.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            12776..13906
                     /locus_tag="VW191_000548"
     CDS             12776..13906
                     /locus_tag="VW191_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEU9623365.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDDAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            13918..14457
                     /locus_tag="VW191_000549"
     CDS             13918..14457
                     /locus_tag="VW191_000549"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU9623366.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVAYDPQQLVKTSFALLEPQ
                     GDVEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGRTLSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            14441..15118
                     /locus_tag="VW191_000550"
     CDS             14441..15118
                     /locus_tag="VW191_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU9623367.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(15185..16084)
                     /gene="galU"
                     /locus_tag="VW191_000551"
     CDS             complement(15185..16084)
                     /gene="galU"
                     /locus_tag="VW191_000551"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEU9623368.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYKRTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(16106..17122)
                     /locus_tag="VW191_000552"
     CDS             complement(16106..17122)
                     /locus_tag="VW191_000552"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9623369.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            17334..17774
                     /locus_tag="VW191_000553"
     CDS             17334..17774
                     /locus_tag="VW191_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9623370.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLEVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVFDK
                     IFEEYYDRLPEEERFIIPNYSYLALANYTVQKLPEKLVEILSFW"
     gene            complement(17897..18313)
                     /locus_tag="VW191_000554"
     CDS             complement(17897..18313)
                     /locus_tag="VW191_000554"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9623371.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTA"
     gene            complement(18381..19031)
                     /gene="phoU"
                     /locus_tag="VW191_000555"
     CDS             complement(18381..19031)
                     /gene="phoU"
                     /locus_tag="VW191_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU9623372.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(19046..19798)
                     /gene="pstB"
                     /locus_tag="VW191_000556"
     CDS             complement(19046..19798)
                     /gene="pstB"
                     /locus_tag="VW191_000556"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU9623373.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(19800..20615)
                     /gene="pstA"
                     /locus_tag="VW191_000557"
     CDS             complement(19800..20615)
                     /gene="pstA"
                     /locus_tag="VW191_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU9623374.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(20608..21471)
                     /gene="pstC"
                     /locus_tag="VW191_000558"
     CDS             complement(20608..21471)
                     /gene="pstC"
                     /locus_tag="VW191_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU9623375.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(21589..22464)
                     /locus_tag="VW191_000559"
     CDS             complement(21589..22464)
                     /locus_tag="VW191_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEU9623376.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(22631..23962)
                     /locus_tag="VW191_000560"
     CDS             complement(22631..23962)
                     /locus_tag="VW191_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEU9623377.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(23963..24670)
                     /locus_tag="VW191_000561"
     CDS             complement(23963..24670)
                     /locus_tag="VW191_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623378.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(24754..25230)
                     /locus_tag="VW191_000562"
     CDS             complement(24754..25230)
                     /locus_tag="VW191_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU9623379.1"
                     /translation="MDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALYTSI
                     YFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKSTIWGSTVDKDDALVSCLVAPGFEFEDFELFERADLLATYPEHKEMIEHLTRY"
     gene            25237..25764
                     /locus_tag="VW191_000563"
                     /pseudo
     CDS             25237..25764
                     /locus_tag="VW191_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26079..27770)
                     /locus_tag="VW191_000564"
     CDS             complement(26079..27770)
                     /locus_tag="VW191_000564"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU9623380.1"
                     /translation="MNTKELIASELASVIDSLDQEAILNLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKINSQSFEKVVATGPYVNFFLDKSAISAQVLQAVITEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGNEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFSRLYNELQVEFDSYNGEAFYNDKMDAIVDILSEKGLL
                     VESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAISR
                     AKAQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQAFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            27906..28352
                     /gene="argR"
                     /locus_tag="VW191_000565"
     CDS             27906..28352
                     /gene="argR"
                     /locus_tag="VW191_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9623381.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVFVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLANETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDANKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            28403..30937
                     /gene="mutS"
                     /locus_tag="VW191_000566"
     CDS             28403..30937
                     /gene="mutS"
                     /locus_tag="VW191_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU9623382.1"
                     /translation="MATEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDRDGSQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKEGFEDLHLLDSRLASVEQA
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIIQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDGIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRRVLREGTGWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            31083..32777
                     /gene="patA"
                     /locus_tag="VW191_000567"
     CDS             31083..32777
                     /gene="patA"
                     /locus_tag="VW191_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEU9623383.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(33107..33370)
                     /locus_tag="VW191_000568"
                     /pseudo
     CDS             complement(33107..33370)
                     /locus_tag="VW191_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            33568..35334
                     /gene="patB"
                     /locus_tag="VW191_000569"
     CDS             33568..35334
                     /gene="patB"
                     /locus_tag="VW191_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEU9623384.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEIRHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            35473..36162
                     /locus_tag="VW191_000570"
     CDS             35473..36162
                     /locus_tag="VW191_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9623385.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            36166..36345
                     /locus_tag="VW191_000571"
     CDS             36166..36345
                     /locus_tag="VW191_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623386.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            36332..37681
                     /locus_tag="VW191_000572"
     CDS             36332..37681
                     /locus_tag="VW191_000572"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU9623387.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            37805..39265
                     /locus_tag="VW191_000573"
     CDS             37805..39265
                     /locus_tag="VW191_000573"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU9623388.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            39279..39713
                     /locus_tag="VW191_000574"
     CDS             39279..39713
                     /locus_tag="VW191_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU9623389.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACRKFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        11640 a         7537 c         8825 g        11758 t
ORIGIN      
        1 taacaaatag aaagatgagg tatatagaat gcccctacct actggtaaga ttgatttaaa
       61 cgaatacggg aagcatttag cagaaaagtc agctgagatt tacaaagaga aaactaaaca
      121 agcagttgac gcgatggagc gacatagaca agaatgttta gcaagagacg ccaagtggca
      181 agctgtcata gatcgttccg atgaactatt aaaagaaaag aaggaaagag agcaccagca
      241 agagattgaa cgcttgatgg aagagaagcg caaagaggtt gatagagaag ttagtcagcg
      301 cctatcaatg ccaaatgaac accagaaaaa actgaacaaa gcgatgagtg aaatgttgaa
      361 agatttattt taaagagaac ataaaaagac tccataaaaa aataattaaa aagtttttta
      421 ataaagtgaa tcggaaaaat attttttgtg aaagagattg gagaagcaag ggagaaggag
      481 gggggatacc cccctccccc tcggagctcc cgaacttccc accatcactg tacatttttt
      541 ctcgcgggaa atgaaaggta gttgtataaa atgacattga aaggtatggg ctatctcagg
      601 aagaagctag ccaattataa aatgggtgta gatactagat acaatcagta tgctatgcaa
      661 cacaatgaca tagatgttgg tattacgata ccacctcaaa tcaggcaaca atatcgagcg
      721 gtcttaggtt ggactgctaa gggtgttgac agtcttgcag atcgtttggt ctttcgtgag
      781 tttgccaatg atgagtttgg agcgaatgaa atctttgctc agaacaatcc agatgtattc
      841 tttgatagcg cgattctttc ggctctgatt gggtcgtgtt gttttgttta catctcgcaa
      901 ggggacgatg atgacgctcc tcggttgcag gttatcgagg cgagtaatgc aactggtgtt
      961 ctggatccta tcactggctt gttgatagag ggctatgccg ttttgaaaag ggatgacaat
     1021 ggttatgccg tgcttgaggc ttattttact agtgatgtga cttggttcta tccgaaagat
     1081 ggtaagccgt ttgcaatcga aaatccgacg ggtgttcctt tgctggtgcc agtcattcat
     1141 aggcctgata ctgttcggcc gtttggtcgg tcacgaatta ctaaggctgg gatgtactat
     1201 cagagatatg ctaaacgaac gcttatgcgg tcagatgtta ctgctgagtt ctattcattc
     1261 cctcagaagt atgtgttggg attgagtcaa gatgctgagg cgattgatac ttggaaagca
     1321 actgtatcta gcttgctgac gtttacgaaa gatgatgagg gggaaaagcc gaatgtggga
     1381 caattcacca cgtccagcat gtctcctttt actgagcagt tacggactgc agcagctgga
     1441 ttttctgggg aaatgggatt gaccttggat gatcttggtt ttgtttctga caatccgtca
     1501 tccgttgaag ccatcaaggc tagtcatgag aacttgcgtt tagctggtcg gaaggctcag
     1561 cgctctctgg gttctggtct gctgaatgtg gcttatgtcg ctacttgttt atgtgatgag
     1621 tttccttatt tgaggaaaca gttcaataaa acggtcgtga agtgggagcc tttatttgag
     1681 gcggacgcta acatgctgac cttgattggt gatggtgtta tcaaactgaa tcaagcggtg
     1741 cctggctata tggatgctga aaccatccgt gacttgactg gaattaaggg gtcagacaag
     1801 cctgctccag tagtgaagga gggtgcagat ggtggaagat atgtttccga gtctactcca
     1861 gaaaattaag gacgaatttg aaaaaagcta ggcttgatag tgagattctg gaagagttac
     1921 tagaaaagaa taaagttaat tatctggacg ccaatcaata tgctatagaa attggtgaaa
     1981 tactcgcaaa ggtcttagga gcttacttga gtaatatcaa tcaagaagat gtggagcgtc
     2041 gaaaacagat aggtatagag tctgctgaag aacgtaaaag aaaacgagat gaaaggattg
     2101 ccaagtttgg aaatcctaga agaagattaa actaggcata agttacagga gtagaaaggt
     2161 tgtcgttaaa aatggagctg gataagttta aaacgatgat gaacgtcaga aagcggatga
     2221 cttactttct gagatttcag aggatggcag gtagtgaaaa ccaagttagg atagatgaag
     2281 aggcttggaa acttgtctta cctgatcagt ggcatttgag cggtgagcat gaaaaagcaa
     2341 tccgtgaggg gttggagata ttcgcccacg acatcaacag catagagaac gagagagcca
     2401 gaaaatactt cattatccat tattgctata tgagaaagaa aactatgagc gaatgcgtag
     2461 aaatggcagg tactagctcc actagctacc accgatacaa acagatatcc gtcttaaact
     2521 ttgcaagaat ccaccagaac ggagagctag aagcatataa gtaggctgag gctgcatatt
     2581 cgccccctta tttcgctttg tttcgtactg gacaactaaa acaccataga taaactagaa
     2641 gcccttagaa acgaatctgg ggggcttttg tgaggtcata atcttataaa ctacgcgctt
     2701 catggtataa tatacctatc agcaatcagc aataaaaaag cacgtttgac cgtgctagtt
     2761 tcttgcctgc tgaactcgtc atttattgcc cttttgtggg gctttttttt gtggactttt
     2821 tgagaatttt taagaacttt taatgatttc taatcaaatg agatttttca aaaagtcagt
     2881 aatatcaagg gtttaggcgc tatattgagg tgtgaaatga tgcctaagtc aaattattat
     2941 atactgttat tatagtcaag aaaaatatgc tattatgtta aaaaaatatt tttcaaaata
     3001 taaatggacg gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa
     3061 ccatgatttg tttactctca atcatcaaga attctctttt cgtggtagcg tttggggtct
     3121 ggtactggcc ttatatcact tattgttcat tgataagttt gtaatttcga atcgaaaata
     3181 aagattagag ctatgcttga ctgtgtactt ttaggattga ttttggagga agattttgtc
     3241 tctattattt attattttaa atttatttat tttgtataag atctattctt tgaggcggga
     3301 aaaatcgaaa tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg
     3361 tattcaatgg ttattaagag aactgatttc aactatttcc ccttaatgtg tatggaaact
     3421 ataacaaaac gcataagatt aaggtttgtg tacctgttct tatgcgtttt tgtcatattg
     3481 aagattttcg gtaagtttta tacctttggg aagtctagtt tagaatgctt tcccaaccag
     3541 aatctctgct tcgatggtaa tctctgtcag ttggctccag tcaatcttgc tctggtcaac
     3601 atgaaagagg agaggagtca tactgagtag ggcttggagt tgttctgctg tgatagtctt
     3661 agtcagagag gcagtttgac tagataggat ggtaaagtgt tcctggaaat gctccttgat
     3721 atcttggtta gaatactcct tgtttgtcag ctggtcctgt actctttgac ggatttcttt
     3781 gaggtgattt tcagttggga taacctttat caagataccg tctttggata aaacgcgacg
     3841 aaattctcca tagttggcag gtgagaagat atcaagcaga atatccatgt tagcgtcttt
     3901 tataggaagg catgccaagt cgccaacgaa ccaattgact gcccagttgg gttcactttt
     3961 agcagcgatt tggactgaat ctttggagat gtcaaaggca tagaaagttt tttcagagtg
     4021 actttcttgt agtttgcgag aatagaatcc ttcaccacaa ccgatatcca aaattgttgt
     4081 ggtagttttt gagcttgcaa gcaagtcaga tacagcatct aagatagctt ggtaaaagcc
     4141 ggcttctagg atttgttgac ggttttgaaa attttccttg tcatagttag cagattgctt
     4201 gatttgaggt gctagattga catagccaaa tttcgccaag tcaaaagaat gacggttgca
     4261 gcacttgaaa ttagtctcta acagagtcag attttcttga cagataggac aggcaaaggc
     4321 agtcgcagaa gcaaaacgct gaagtttggg cttgagattt gtattcatag tttaagtgta
     4381 tcaaaagagg caaatgaaag caactttagg aataagtaga tgggagattc agtagaaata
     4441 aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc tatataataa
     4501 tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag ttgcgaaaaa
     4561 atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa tcttagcaag
     4621 taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt tgaaagacga
     4681 gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc aagatgccaa
     4741 atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag gtgaataata
     4801 tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct tctttttcac
     4861 ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag gggattgagt
     4921 ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa ggcttttctg
     4981 tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt tctgagttga
     5041 tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt gcagcggtta
     5101 agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg acttgttatc
     5161 aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa ctaacgatgg
     5221 aagtcttgga ctgttcggct attttattgt ccttgtttat gaaccaatcc aagacagcta
     5281 gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc ttgaaaaaaa
     5341 ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc ctagtacagg
     5401 gcgatacggt tgttagtata aagagttccg atgttcaaat aggagatgtc ttgatcttat
     5461 ctcaaggaaa tgaaattctg tttgatgggc aagtagtttc aggtttaggt atggtcaacg
     5521 aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat ttggtttgtg
     5581 caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat cagatgaaca
     5641 gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag aaaacgaaac
     5701 aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg ggggctgggc
     5761 tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg gtcgatttct
     5821 cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag gaggggttga
     5881 accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa gttgatactt
     5941 tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa aaggtgttac
     6001 cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt gaggaacaca
     6061 tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga attgaacatg
     6121 aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct catatagatg
     6181 gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt catatcagtt
     6241 cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta ttcttggctt
     6301 atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa gaagcaaaaa
     6361 cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca ggagacacct
     6421 tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat acagacttga
     6481 aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac actattttga
     6541 tggttggaga tggcttgaat gactcagcgg ctttaactct atcagatatc ggtgtggtga
     6601 tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta gataatcgtt
     6661 tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc atcaagaaaa
     6721 atattcagga taccgttgtc gtaaatagta gtttgattgg ctttggctta tttaattggt
     6781 tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt gtcctgcgta
     6841 gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag ttacctttct
     6901 cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc aatacaaagt
     6961 attttttctt cccacgacgg ataacagtca gttcattttc taacttatca gcgtcactca
     7021 agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg ttttggacgt
     7081 cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt tccacgatat
     7141 tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct tgtttgagct
     7201 ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg ttaagtgctt
     7261 ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag actttttgag
     7321 ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc tcatcaagtg
     7381 acaagaaagt aaagattttc aagaagcgaa cagcgtcagc gtccatcacg ttcatccaga
     7441 attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca tttccttctg
     7501 atttaccaaa tttcttacca gttgcatctg tgattagtgg aacagtgata acgtgaccag
     7561 tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc cactggtcag
     7621 aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag tcgtaccctt
     7681 gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt tttttaacag
     7741 attccttact catcatgtag ttgaccgtga agtattttcc aatatcacgg aggaagtcaa
     7801 tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg ttttcgccat
     7861 tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag ccatctactg
     7921 tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg atgagacctg
     7981 tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga cgacttgtca
     8041 agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag ccagtatagt
     8101 aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc gtttgaaata
     8161 tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc tttttaaaat
     8221 tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat agggaagaaa
     8281 gtggctattt tattgaaaat ttcccttttg tggtataata gataaagtga ggaaatccat
     8341 gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc aaaaacaaaa
     8401 aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat ctaaaacctt
     8461 agataagtca gccattttcc cagctatttt actgagtata aaagccttat ttaacttact
     8521 ctttgtactc tgttttctag gaggaatgtt gggagttggg attgctttgg ggtacggagt
     8581 ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc aggtcaagga
     8641 catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt ccatagagag
     8701 tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga agaaggctat
     8761 cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca aggcggtgat
     8821 tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt caaccttgac
     8881 ccagcaacta attaaacagc aggtggttgg ggatgcgccg accttggctc gtaaggcggc
     8941 agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga ttttaacgac
     9001 ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg caggggctcg
     9061 gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc ctcaagcagc
     9121 atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa atactgggga
     9181 gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag ttctttacag
     9241 tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg attatgacct
     9301 taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact atttatactt
     9361 tacaactttg gcagaagctc aagaacgtat gtatgactat ctagctcaga gagacaatgt
     9421 ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt tggcagccaa
     9481 ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa ttcattctgc
     9541 catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag gtcgtgtaga
     9601 agtagggaat gtcttgatgg acaaccaaac aggtgctatt ctaggctttg taggtggtcg
     9661 taattatcaa gaaaatcaaa ataatcatgc ctttgatacc aaacgttcgc cagcttctac
     9721 taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg gaagtgaaac
     9781 gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt atgctaatag
     9841 caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga atatccctgc
     9901 ttactggacc tatcgtatgc tccgtgaaaa tggtgttgat gtcaagggtt atatggaaaa
     9961 gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg gtggtattga
    10021 agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg gagtttatca
    10081 tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt atgagtatca
    10141 ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg gattgctacg
    10201 agaagttcta tcttctcgtg tgacaacaac cttcaagtct aacctgactt ctttaaatcc
    10261 tactctggct actgcagatt ggattgggaa gactggtaca accaaccaag acgaaaatat
    10321 gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc atgatgataa
    10381 tcattcattg tcacgtagag caggttattc taataactct aattacatgg ctcatctggt
    10441 aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg ctttagatcc
    10501 tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga aggtttctgt
    10561 tgaaggaaaa gaagtagagg tcacaggttc gactgttacc agctattggg ctaataagtc
    10621 aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg attatcagaa
    10681 tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca gttcaagtag
    10741 tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa gggcgagacg
    10801 ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcttt tcgtgttaca
    10861 ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat tcgctcctat
    10921 ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact cattatcagg
    10981 tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt gtactcattt
    11041 ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag aaaaattacc
    11101 tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt ttctatctct
    11161 gtgtagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc cctgtcttta
    11221 tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct gttttctatg
    11281 ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagacctc tctcagttat
    11341 ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt atgtttaatg
    11401 ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt gggtggggga
    11461 tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt tcctttactc
    11521 ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg gcttttgctt
    11581 ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggttgcc gttatcagtg
    11641 ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta gtagtggatt
    11701 ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg tctatagaag
    11761 aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct atactggaat
    11821 agtcaataaa acacggggag atagttgtga agagtgtttt taggttgtat cggtaggggc
    11881 ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc taaaaacaga
    11941 caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat aacgaaccag
    12001 gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt tatcgccaat
    12061 gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac tgctgtgcct
    12121 agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt ggctccgctt
    12181 cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg taattctgca
    12241 gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc tattatccgt
    12301 gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat caatatcggt
    12361 gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg ccgtgccatc
    12421 gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat tgagccagct
    12481 agtgctgaac cagtccgtgt cggagacaat gttctcatcg gggctaatgc agtggttatc
    12541 gaaggagtcc aaatcggtag tggttcagtt gtcgcagcag gagctattgt tacccaagat
    12601 gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga aattgatgcc
    12661 caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt gtaaaagtaa
    12721 aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca gatagatgtt
    12781 agatttgatt cagactagac gagatttaca ccagattcca gagattggct tggaggagtt
    12841 caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca aggattttgt
    12901 tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc agccggagcg
    12961 aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa caggtctacc
    13021 ttttgcttct caacaccaag gtcgcatgca tgcctgtggt catgattttc atatgaccat
    13081 tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc tgctctttct
    13141 atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg acgatgcttt
    13201 tggagattgg ttgccagatc aattctatgg tctccatgtt cgtccagatt tgaaagtcgg
    13261 ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga agatccgttt
    13321 caaaggcaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct tggtggcggc
    13381 tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc caatcgaggg
    13441 agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca tcacagacac
    13501 agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct tggtgcaaaa
    13561 gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag tcgaagtgga
    13621 actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc gtgaactgat
    13681 ggacttcttt gacgagaaag acggaatcga gttgattgat atcgaacctg ctatgacagg
    13741 cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc taggtatcga
    13801 tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct tagctattgg
    13861 ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg cttgtctatg
    13921 aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca agagcaaaaa
    13981 caggctatag accaagcttt aaccgagcgg cttttacacc atcccttcta ccaagaagcc
    14041 aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga actgattgag
    14101 caggcgctga aggacggcaa gaaggtttta atacccaaaa cttatcccaa ggggcgcatg
    14161 gagtttgtgg cctatgatcc gcaacagttg gtaaaaactt cctttgcctt actggagcca
    14221 cagggagatg tggaagtggt ggatgcatct cagattgatt tgattcatgt tcctggtctg
    14281 gcttttacga cgaaaggata tcggatagga tacggtggag gctattatga ccgctatctg
    14341 gaacattttt ctggtcgtac tttgagtacg atatatcctt gtcaaattca ggactttata
    14401 cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa tctttgatag
    14461 acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt tactaatgtt
    14521 ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg gagccatgta
    14581 tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg ccatttttgt
    14641 tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc ttggaaggca
    14701 ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat caggaatgat
    14761 gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg cctctacctc
    14821 tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca atccttatat
    14881 ccaacagcta gggcaatcct atctgacact ttttgtggtt aacattattg gaagtgttct
    14941 gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg catttctagc
    15001 agttatcttt ccagttagag gagaaaaacg gatgtataac accagccaga gattaggagc
    15061 ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg gaatgtgacg
    15121 aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac cttatctttt
    15181 tattttattc cttctcagtc aattcttttc caagttggat gaggtaattc ttcaaatcat
    15241 ctttgacttg tggatgtttg agggcgtagt caatagatgt tttcataaag ccaaacttat
    15301 ccccaacgtc gtaacgagct cctgtaaatt cacgggcaaa aacacgctgt gttttattaa
    15361 gagtatcgat tgcatcagta agttggattt catttccagc accaggagct tggttttcga
    15421 ggatttggaa aatttcaggc gtgaggaggt agcgtccgat aatagcaagg tcgctaggag
    15481 cgtcctctgg agctggtttt tcaacaaagg tttcaacact gtaaagacca tcttttcctt
    15541 cgccttgcgg agcaataacc ccgtaagcag atacttcgtc atgagggact ggcatgacag
    15601 cgatagtaga cgcgtgggta cgcttgtagt catccatgag ttgtttggta agtggaacag
    15661 ccttttcgtc tgtgatatcc atcaagtcat caccaagcat aacgacaaaa ggttcatttc
    15721 cgacgaaagc cttggcttgc aaaacagcat ctccgagacc gcgtggatga gtttggcgga
    15781 taaaatgcag acgcatgcca gttgtttcat caactagctt caaaagatct gttttccctt
    15841 tttctttgag gttatattcc aattcgaagt ttgaatcgaa gtggtcctca atagaacgtt
    15901 ttgatttacc agtgacaact agaatatctt caatacctga tttgagagcc tcttccacga
    15961 taaactggat agttggtttg tctacgattg gcaacatttc tttggcaagg gccttggttg
    16021 ctggtaaaaa tcgagttcct agtccagcag cagggataac tgcctttcta acttttgatg
    16081 tcataaaaat cctttctata gagggttaag accactcatt ttctgcttta aattcattgt
    16141 tcatgatgtc ataaatggca tctttgatat tggttccgtg ataaataact tggtaaatag
    16201 cctgtgtaat gggcatatag actccaagtt cttgggctag ttcataggct gctcgagtcg
    16261 ttgaaattcc ttcgattacc atgcccatat tagcttctat atcagctagg gattctcctc
    16321 gtccgagagc atctccagct ctccagttac gagagtggat ggaagttccc gttacgatca
    16381 aatctcccac accagataag ccgctatagg tcaatggact ggccccgagt gctaccccta
    16441 ggcgggtgat ttctgctaaa cctcgagcga tgatggctgc cttagcatta tcaccaaatc
    16501 caagaccatg taaagctcca gcaccgacag caataatatt tttaagagca ccagcagttt
    16561 caaccccgat aacatccgta ttggtataaa gtcggaagta gtgattacta aatagctcct
    16621 gaacgtattg agctgtttgt aaatctttag aagcagcagt tattaaagtt aggtcacgca
    16681 caatggtctc ttctgcatga ctaggccctg aaacaacgac gatatcacta cggagatgtt
    16741 caggaatttc ttcttcaaga atggttgata atcgtttatg gctatcaggt tctaatccct
    16801 ttgatgcgtg catgatgata accttatggt ccaaggtttg tgcaacttgc tgggcaacaa
    16861 gtcgtgtcac ttttgttggg acaacaaaca aaatcgcatc cacatttttc aatgtttctg
    16921 ctaagtcggt gtaggcaatg atattttcgt ctagaacgac atctttaaag tagtgcttat
    16981 tagtatggtg tgtattaatt tcattgattt gctcgggaag atttccccaa atacgtacct
    17041 cgtgtccatt gtcatttaag acttgtgaaa gggcggttcc ccaagaacca ggccccaaga
    17101 cggcgacggt ttgtttttcc atgatttcct ccttgataga tttctttctc ttattctatc
    17161 atattctagg ggattttgca cttgcattac tggggtatgg tggaaaaata cacaaagacc
    17221 gagatgaagg gataaactct tagtctttag ttaaagtgag aagaaatctg atagatagtt
    17281 tgattcagta tatgcttata atagaggtaa acaactcagg aagttcttgt aggttgcgag
    17341 agtttggcga aaaaattaaa agattacgtt tggctaaaaa aatcagtcgt tcagaatttt
    17401 gtggtgatga gtctgaatta agtatccgtc aattaattag aattgaaaat ggagaatcca
    17461 gaccaacact aacaaagtta aaatatattg ctgaacgttt ggaggttgaa gattacaagt
    17521 tgatgccaag ttatatagag ttggataagg aatacctaga attgaagtat ttcttgatga
    17581 ggactcctac atacgaagat gaaactatcg cccaaaagaa agagagtgtt tttgataaga
    17641 tttttgaaga gtattatgat aggctacctg aggaagaaag atttatcatc ccaaattatt
    17701 catatctagc actagcgaac tacacagttc aaaaattacc agaaaagcta gttgaaatac
    17761 tgtccttctg gtgattttgc ttaatttctg atgttcgaga catgattgag gagatttgtc
    17821 ttgatttttt cctaaaaaag ggcagactcc tcccttggtt tgatgctcga tttttaaaat
    17881 aaggtgactt attctgttag gcagttttcc cctgccctct agctctatat ggaacaggga
    17941 gtatcagatt tatctggcag attgtatcaa ataaggcttg aaactgtttt gaataggcgt
    18001 atagacttga gaatttgaga atatgtcgtc tagcagtaga gacatatttt ccggcaatat
    18061 gaaggaagag acgtcgaaaa cgcttgatag tcaaggcctt tacttcatca ccagctagct
    18121 gctttaaaaa gaggtagaga ttataagcca gacagcccat catcatacgt acttcattct
    18181 taatcatggt cgaactatct gtcttatccc caaagaatcc tgctttcctt tctttgataa
    18241 agttttcagc attccctcgt tcacgataga attgaaacag ctcctcacta tctacactgc
    18301 ttaaattggt caaatgaact tttttacaac cataaaaaag agcacttaat cgtactcttc
    18361 tcttttaagg attagttgaa ttaattcaaa tccactagtt ctcctgtttc taggtagact
    18421 aggcgttcac aaatgttagc aatgtaatca gcgaagcgtt ccagatgccc tatgatataa
    18481 aggtattgag ttccattggg aattgaggtt tcttggtctt tcataagtcc aatgatttcc
    18541 tttgataagg cataataata ttggtcaatc tgttcatctt tttgagcaat actaatagct
    18601 tttgaggctt ggtgcaaagg aaaggcaacc aataaatcgg ctagcatgct gagggataat
    18661 ttacccattt ggtgtaactg ttcttcgtca ggggctagtt gattttcttt tagttgcaaa
    18721 acagctttgg caatgcctgc catatggtct cccatacgtt caaggtctga acaagaagac
    18781 atgatgctaa tcacaaatcg aaggtcagac acttgtggct gctgcaaggc caacaaacgg
    18841 gcacaggtca attcgatagc gctttgacct tggttgatag catgatcctt attgataatt
    18901 agctctgcca tctccttgtc tttggaggct aaggccagta aggcttttga agctgtttca
    18961 aggacaagtt gccctagtcc taaaaaggat tgttctaatt catgcaattc taagtcaaat
    19021 tgatttctca taggtttttc cttccttatc cgaaccgtcc tgaaatatag tcttctgtgc
    19081 gctgatcttt tggattggta aacacgtcaa cggtatctcc aaattcgcaa atttctcctg
    19141 ttaagaaaaa agcagtttta tctgaaatac gtgaagcttg ttgcatgtta tgggtaacaa
    19201 tgataatcgt ataatccttt tttagttgct gaatgaggtc ttcaattttt aaagtggaga
    19261 tagggtctaa ggctgaagtc ggctcatcca ttaacagaat atcaggttct actgctaaag
    19321 ctcgcgcaat gcaaaggcgt tgctgctgac cgccagataa ggacatagca ctctttttaa
    19381 gatcatcttt gacttcttcc caaatggctg ccccttttaa agatttctcc actaaggcat
    19441 ctaattgttt tttgtctcga ataccatgtg tccttgggcc ataagccacg ttatcataga
    19501 tagacatggc aaagggatta ggctgttgaa aaaccatccc tacacgctta cgtagctgat
    19561 taaggttgaa tttgctacta taaatatctt gctcatctaa gaggacttgg ccttcaatat
    19621 ggcaagaagg aaccaaatcg ttcatccggt taagggtttt tagaaaagtt gatttgccac
    19681 aaccagatgg gcctatcaag gcagtaatct gtctttctgg taattgaatc gaaatatttt
    19741 ttaaggcttg aaaatccccg taaaataagt ctaggtgtct gactgaaaat gttcccatac
    19801 taggaagctc ctttcacaag ttttcgagat aataagcttg atagagtatt tatcattaaa
    19861 acagtaataa tcaaaatcac accggtagca taggcttcat tgacatgtag cccctcactt
    19921 gacagcatat acatatgcag ggctagagaa cgacctgaag acatgagact acttggcgta
    19981 ttggtagagg tacctaatgt atacatgagg gcagctgtct caccaacgat acggccaata
    20041 gctagtatca ctccagctaa aatacctggc atggcaactg gtagaacaat tctaaaaaca
    20101 gtccgtaact tacctgcccc aagtccataa cttgcttgac gcatgctatc actaacagat
    20161 aaaagggctt cttctgttga gcgaataatg actggcaaca ccatgatgac tgaggttaag
    20221 attcctgata acagagagta ttgaaaacct aagaagacta caaagaagag catgccaaac
    20281 agaccaaaaa caatggaagg aatcccagac aaggtatctg aggccaatcg catgatttta
    20341 acacaaaggg aatctttttt tgtatattcc acaagataaa aaccagcaaa aatccctatg
    20401 ggcaaggcta aaagaagagc accaaagacc agaataacgg tggaaataat cgctggcata
    20461 agggaaatgt tctcagaagt ataagtccaa gaaaagaggg atagacttag atgaggtaag
    20521 cctttgatga ggataaaacc aatgattaaa aagagagagc caaaggttaa agctgaaaaa
    20581 caataaacga gaagttttag caggtattta ctcataagat gattttcctt tcaagtaggc
    20641 aaagtaggca ttaatcaaga gaataaggaa aaagagaact gctgaggttg caataagggc
    20701 ttccctatgc tgacctgatg cgtaagccat ttccagaaca atattggttg ttaaggttct
    20761 ggttcctgaa aagagtccac ttggaataat cggctggttg cctgccacca aaataactgc
    20821 catggtttca cctactgcgc gaccgattcc taaaataact gctgataaaa taccagatct
    20881 cgcagctggc aagatgacac taaaaatact ccgttcatga ctagctccta gagccaagct
    20941 accagaataa tacgttttgg gaactgttcg gatagcagat tctgacaaac tgataatggt
    21001 tggcaaaatc attattccta atagtaacga agcggttagg acactcatgc catttcctaa
    21061 aaagcttcta atccaaggca ccaataattg taggccgaaa aaaccataaa caatagatgg
    21121 aatggccgcc atcaagttga tagctgattt taagaagcca tagacgggct ttggacaata
    21181 ataaaccata aacaccgatg tcaagatgcc tgttggcacc ccaatcacaa tcgctcctaa
    21241 ggtaattaat aaggaaccaa cgatcattgg taaaatacca tagcttgccg gaatgttcgt
    21301 tggcgaccaa tcactgccta ataaaaaacg ggcaaagccg tagttagcca tgaaaggtaa
    21361 gccattacta aaaataaaga aacagattag caaaatagct acaacagcta ctgttgcact
    21421 catgaaaaaa attgccctaa aaactgcttc tttgaaggct tgttttatca catcttgtcc
    21481 tttctagtga agaaagtaag ggagatacga cacctcccta cttgccttct ttatcttatt
    21541 gtacgatgaa acgtctgcat ctctttagag atttatggag caaacatttt atttaatctt
    21601 gtcccaggtg gttaatttgc cactaaaaac gtctgcaagt tcagccatac tgacttggct
    21661 tgccttattg tcattattga ccacaacagc aataccgtct aaagcaatag catcatgggt
    21721 gagactctta ccttcttcag gagttaattc cctagaaacc ataccaatat cagcggtttt
    21781 ctccttaaca gcggtaatac ctgctgaaga cccattagag gtaatatcaa tcgtaacttc
    21841 tggattttct tttttataag cttctgctaa tttttccatt aaagaagata ctgaagtgga
    21901 acctacaaca gacaacttgc ctgataagtg ttggcttgta tattctgtgg tttcagtttt
    21961 agcttcaata aatttattat ctgtgaccac ttgttgacct tgtttggagt ggataaagct
    22021 gataaaatct tgacctagct tggaaagatt agaagaccaa acaatgttga agggacgttg
    22081 aagagggtat tcaccatcta aaactgtgtc tcgactagcc ttgacaccat caatctctaa
    22141 agccttgaca gatttcgtta aagatcccaa ggagatgtag ccgatagcat tagcattccc
    22201 ttgaactgct gagagaacac cttctgtact attttgaatc acagctgttt tggcagtgtt
    22261 gtcaattttt ttatcaccgt cttttttgag aatccctgtg atttctgtga aggcaccccg
    22321 tgttccagag ccattttctc gtgaaatcac ctcaatcgtt cctgaagctg actgtttgga
    22381 agcagctgac tgattgccac aggcaacaag cccaaatcct gataagccaa tggctgcaag
    22441 agtaagcatt tttttgaatt tcataataat cacctttatc tctatgtatt tttcttgtgt
    22501 aggcttacta catttatagt ctaacaagtc tttgtaaagg tttatccctg attcatgtaa
    22561 agattgtgta aagaatcaaa aaaagccact tttgaaaaat ggctgctcct aaaaatagct
    22621 ttaaaaatta ttagtcctgt gcgaaagatt ggttaggaag aaaaatcgtg aagcaactgc
    22681 ctctgccaag ctgacttgtc accgtgactt ggccacctaa taattgactg agttctttga
    22741 caatggcaag gccaagacca gtgccaccag tttgtctgct tcgaccttta ttaactcggt
    22801 aaaaacgttc aaaaatacga tcctgctcta attgactaat accaatccct gtatctgata
    22861 cagaaatctt aatgccttcg ttcacctttt gggtcttgac ctcaattttt cccccttgtt
    22921 cagtgtaacg gatggcattg gataaaagat tgagtaagat ttgggaaagt aattgactat
    22981 ctgatacgag ggtgacatca tctggcacct gcacctttag ctgtaaatcc ttcttcttga
    23041 gctgaggttg caagctttga gtcaaatcct gtacaaattc tgccaaagaa agggtcgtcc
    23101 attgtatagg catttgttga gccttagata aggtaagaag atgctcaaca atatgctcaa
    23161 gacgcaagct ttctttgtaa ataatgtcta gaaagtcatc cttgagcgct tcttcttcag
    23221 ctgacatccc cttaatggtt tcagcaaagc ccttaatcga agtaactggt gtcctcaatt
    23281 catgggaggc atttgagaca aaggctaaat ttaacttttc ataagttcta atcgttgtta
    23341 aatcatatag caagacgagc acagcttcca cagattgggt ggggctaaaa acgggaactg
    23401 ctgtcacttc taaaatcaag tcaccctcat gaaacccact tacttcttgt tttaaccttg
    23461 ttttttgatc aaaggcttgg tgaactaaat ttcgaatatc catccgtttg aggtcatcaa
    23521 gtgaacttat gtcgccgtcc acatcgggaa aataatgagg cagagagcga ctggataata
    23581 acatctgacc ttgagcggaa actaaaaacg tccccatggt taggtgcgac agaagaacct
    23641 ccattgtttc ggctaggtcc ttgtattgct gatcctgttg ggagactttg gtttttaggc
    23701 cagacacata ctgagccaaa gactttaagt cttcttgccc tttttctaaa aagtattcac
    23761 tactggtcaa gagaggttgg tgcaaggtct caaaagcaac ttcccatttc caaaggcaaa
    23821 agagccagta gccaccttgt cccaaagaaa gggctagaag aaagagaccg atgcctttac
    23881 tgatccaagt taatgccatc cctgcaatca gaatgaggct aacacttaga ttgactagcc
    23941 aaaattgaag gtagcgtttc atctataact ccttgaactt ataaccataa ccccgaatgg
    24001 ttcgaataaa ttgaggggct ttaggattgt cttcaatttt ttccctcaac ttaccaatat
    24061 gaacgtccac caaacgtgtt tcctgcccaa agtcataccc ccagatacgt tccaaaagac
    24121 gctctctagt cagtgtcatg ttgggatgtt tcataagata gagcaagagt tcaaattctt
    24181 ttggggtcaa actcagtaac ttattcgcct tgtagacttc atgacgctca gggtatactt
    24241 tcaaggtccc aaatagccaa gaatcgtcag cgatattatc tgaatcatct ccttcttgtt
    24301 ctcctttagt tcgcctgagg acagccttga cacgcgccag caattctcta gggctaaaag
    24361 gcttggtcag gtagtcatca gcccctaatt ccaaggccaa aaccttatca aattcatcac
    24421 ttttcgcaga aaccatcata attggagttt tgacgccttt ggctctcagc cgcttacaaa
    24481 cttccatgcc atctaattgt ggtaacatga tatcaagcaa gataaaatca aagggttctg
    24541 tttctgccaa agctaaggcc ttccgtccat ttgttaccaa ttgagtagaa aagccttcct
    24601 tacttaaatg gtagtcaagc aatttcagaa tgtgttcttc atcatccact aataagactt
    24661 gttttgtcat ctattatctc ctattggtaa cattataaca caattatcag aaatcctaac
    24721 attgctaaat tagattaaat ttgcttatca agactagtat ctggtcaaat gctcaatcat
    24781 ctccttgtgc tctggatagg tcgccagtag atctgccctt tcaaataatt caaaatcctc
    24841 aaattcaaaa ccaggagcaa caagacaaga aaccagagca tcatccttat caactgttga
    24901 tccccaaata gtgctcttag gaacacagta gtgaagttgt tgccctttgg atatgtccag
    24961 gcctaaagtg actgcttcgt agtgaccatc tgctgtaatc atgtgaacag taagtgggga
    25021 tcctgcatga aaataccaga tttcatctgc tgtcaatcgg tgaaaatgtg aaggattcgt
    25081 ttcttctaat aagaaataaa tactggtata aagcgccctt cccttaccag caaggtttat
    25141 agtgtctgaa gctttttttg tttgtctaaa atagccacct tcaacatggg gagctaactc
    25201 tagagttctt atcaagtctt ctttatccat cggggccaat gggttgaagt aactcttgtt
    25261 caaagtggtt ttacgatttc aagaactcct ctcagttctg aggacacggt aatgattgat
    25321 gcgacggaag taaaaatcaa tcgccctaaa aaaagaatta gcgaatgatt ctggtaaaaa
    25381 gaaatttcac gctatgaagg ctcaagcgat tgtcacaagt caagggagaa ttgtttctat
    25441 tgccatgata tgaagttgtt caaaatgagt tgcagaaata tcggacaagc tggtaaaatc
    25501 ttggctgaca gtgattatca agggctcatg aagatatatc ctcaagcaca aactccacgt
    25561 aaatccagca aactcaagcc gctaacagct gaagataaag cctgtaacca tgcgctatcc
    25621 aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa aatgttttca
    25681 acaacctatc gaaatcatcg taaacgcttc ggattacgaa tgaatttgat tgctggtatt
    25741 atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatttggtt aggtgaatta
    25801 gtgaagtgtt taggcaagtg tctctggtta cgacgtcatg gcctctaaat cgattatatt
    25861 taggggtcat gactagtgaa gcagttagct agttcgcata taagcggcta gcgtctaaca
    25921 attaggaact ttagttccaa taactttaag attacgacgt tttaggacat aaatcgatca
    25981 tatttatgtc ctaaaactag tgaagcgcct agccaaagtc cgaataggat ttggcgttag
    26041 ttacttagat tgctttgcaa tcaagtaact ttggcgattt acatcttctc tggcgcttct
    26101 actccgagca agcgaagggc ttctttgaga acgactgcgg ttgcgtagct gagggctaga
    26161 cggctgtcgc gttctgggct ttcatccaag atacgcgtgt gtgcatagta tttgttaaag
    26221 gcttgagcca ggctaattgc aaatttggca atgatagaag gttcaaagtt atctgccgca
    26281 cgattgataa tacgtgggaa gtcttggatg agtttgatga tttcccagct ttcagtatca
    26341 ttcaagctat agttgccggc tgtttctggt ttgaagtctg ctttacgtaa gatagattgg
    26401 atacgagcgt aggcatattg tacataagga ccagtttccc cctcgaagga taccatagct
    26461 tctaggtcga aatcgtatcc atttgtacgg tcggtcttga ggtcatagaa cttaatggct
    26521 ccaaccccaa cagcatgtgc tacctgatct ttattttcca attcaggatt ttttgcttca
    26581 atttgggctt tggcacgact aatagcctct gcaacagtgg gctctagcaa gatgacattt
    26641 cctttacgag tagagagttt tttcccttct tttgtaacca aaccaaaagg aacgtgggta
    26701 atgtcgtcac tccagtcgta gcccatctct tgcaaaacag ctttgagttg tttaaagtgg
    26761 gcagattgtt cttgaccaac gacgtagata gatttggcaa attggtattc gtttttacgg
    26821 taaagggctg cagccaagtc acgtgtgata tagagagttg ctccatcaga tttcttgata
    26881 agggctggat gttcaattcc atatttctca agatttacaa cctgggcacc ttctgattca
    26941 acaagaaggc ctttttcaga aagaatgtct acaattgcat ccatcttatc gttgtagaag
    27001 gcttctccgt tgtagctgtc aaattcaacc tgcaattcat tgtaaaggcg gctaaattcc
    27061 accaaacttt catcacggaa ccattgccaa agagcgagag cttcctcgtc tccattttca
    27121 agtttacgga accattcgcg tgcttcttca tccaagctag ggtcattttc agcttcagcg
    27181 ttgatgcgga catagagttt aagaagttca tcgattggat gagcttttac agcttcttcg
    27241 ttcccccatt ttttgtaggc aacaatcaac atcccaaact gtttacccca gtctcccaaa
    27301 tggttgacct tgaccgtttg ataaccgatt ttttggaaaa tatgtgacaa gctatctccg
    27361 ataacagttg agcgcagatg accaatagag aatggtttag cgatattggg actagacatg
    27421 tcgataacaa cattttcttg tttaccaatg ttttggtcag catagtgttc tttttcagtg
    27481 ataacagctt gtaatacttg agcagaaata gcagatttat caaggaaaaa gttgacgtaa
    27541 ggtcctgttg cgacaacctt ttcaaaggat tggctgttaa tcttttcagc tagttcagcc
    27601 gcaatcattt gaggtgcttt acgttcgact tttgcaagag aaaaagcagg gaaagcaatg
    27661 tctcccattt ctgagttttt aggggtttcc agtaaattta aaatagcctc ttggtccagg
    27721 ctatcaatga cgctagccaa ctcgctagca atcaattctt ttgtattcat taagagctcc
    27781 tttttggact tttctactat tttatcacaa ttttaaagaa agaagaaaaa atttttgaaa
    27841 tctcctattt ttttggtata atatagttat aaaaatagtt ataaatattc acataagagg
    27901 attttatgag aaaaagagat cgtcatcagt taataaaaaa aatgattact gaggagaaat
    27961 taagtacaca aaaagaaatt caagatcggt tggaggcgca taatgttttt gtgacgcaga
    28021 caaccctgtc tcgtgatttg cgcgaaatcg gcttgaccaa ggtcaagaaa aatgatatgg
    28081 tgtattatgt actagcaaat gagacagaaa agattgattt ggtggaattt ttgtctcatc
    28141 atttagaagg tgttgcaaga gcagagttta ccttggtgct tcataccaaa ctgggagaag
    28201 cctctgtttt ggcaaatatt gtagatgcaa acaaggatga atggatttta ggaacagttg
    28261 ctggtgccaa taccttattg gtcatttgtc gagatcagca cgttgccaaa ctcatggaag
    28321 atcgtttgct agatttgatg aaagataagt aaggtcttgg gagttgctct caagacttat
    28381 ttttgacaag gagagacgga aaatggcgac agaaaagcta tcacccggca tgcaacagta
    28441 tgtggatatt aaaaagcaat atccagatgc ttttttgctc tttcgtatgg gtgattttta
    28501 tgaattattt tatgaggatg cggtcaatgc tgcgcagatt ctggaaattt ccttaacgag
    28561 tcgcaacaag aatgccgata atccgatccc tatggcgggt gttccctatc attctgccca
    28621 acagtacatt gatgtcttga ttgagcaggg ttataaggtg gctatcgcag aacagatgga
    28681 agatcctaaa caagcagttg gggttgtgaa acgagaggtt gttcaagtca ttacgccagg
    28741 gacagtggtc gatagcagta agccagacag tcagaacaac ttcttggttg ccatagatcg
    28801 tgatggcagt cagtttgggc tagcttatat ggacctggtg acgggtgact tttatgtgac
    28861 aggtcttttg gatttcacgc tggtttgtgg ggaaatccgt aatctcaagg cgcgagaagt
    28921 ggtgctgggc tatgacttgt ctgaggaaga ggaacaaatc cttagtcgtc agatgaatct
    28981 ggtactttcc tatgaaaaag aaggctttga agaccttcat ttactggatt cacgattggc
    29041 atctgtggag caagcggcat ctagtaaatt gctccagtat gttcatcgga ctcagatgag
    29101 ggaattgaac cacctcaagc ctgttatccg ctatgaaatc aaagattttt tgcagatgga
    29161 ttatgcgacc aaggctagtc tggatttggt tgagaatgct cgttcaggca agaagcaagg
    29221 cagtcttttc tggcttttgg atgaaaccaa aacagccatg ggtatgcgtc tcttgcgttc
    29281 ttggattcat cgccccctga ttgataagga gcgaatcatc caacgtcaag aagtggtgca
    29341 ggtcttcctc gaccatttct ttgagcgcag tgatttgaca gacagtctca agggtgttta
    29401 tgacattgaa cgcttggcta gtcgggtttc ttttggcaag accaatccca aggatctctt
    29461 gcagttggcg actaccttgt ccagtgtgcc acggattcgt gcgattttag aagggatgga
    29521 gcaacctgct ctagcctatc tcatcgcaca actggatgga attcctgagt tggagagttt
    29581 gattagtgca gcgattgctc ctgaagctcc tcatgtgatt acagatgggg gaattatccg
    29641 aactggattt gatgagactt tagacaagta ccgtcgcgtt ctcagagaag ggactggttg
    29701 gattgctgag attgaggcta aggagcgaga aaactctggt atcagcacgc tcaagattga
    29761 ctacaataaa aaggatggct actattttca tgtgaccaat tcgcaactgg gaaatgtgcc
    29821 agcccacttt ttccgcaagg cgacgctgaa aaactcagaa cgctttggaa ccgaagaatt
    29881 agcccgtatc gagggagata tgcttgaggc gcgtgagaag tcagccaacc tcgaatacga
    29941 aatatttatg cgcattcgtg aagaggtcgg caagtacatc cagcgtttac aagctctagc
    30001 ccaaggaatt gcgacggttg atgtcttaca gagtctggcg gttgtggctg aaactcagca
    30061 tttgattcga cctgagtttg gtgacgattc acaaattgat atccggaaag ggcgccatgc
    30121 tgtcgttgaa aaggttatgg gggctcagac ctatattcca aatacgattc agatggcaga
    30181 agataccagt attcaactga ttacagggcc aaacatgagt gggaagtcta cctatatgcg
    30241 tcagttagcc atgacggcgg ttatggccca gctgggttcc tatgttcctg ctgaaagcgc
    30301 ccatttaccg atttttgatg cgatttttac ccgtatcgga gcagcagatg acttggtttc
    30361 gggtcagtcg acctttatgg tggagatgat ggaggccaat aatgccattt cgcatgcgac
    30421 caagaactct ctcattctct ttgatgaatt gggacgtgga actgcaactt atgacgggat
    30481 ggctcttgct cagtccatca tcgaatatat ccatgagcac atcggagcta agaccctctt
    30541 tgcgacccac taccatgagt tgactagtct ggagtctagt ttacaacact tggtcaatgt
    30601 ccacgtggca actctggagc aggatgggca ggttaccttc cttcacaaga ttgaaccggg
    30661 accagctgat aaatcctacg gtatccatgt tgccaagatt gctggcttgc cagcagacct
    30721 tttagcaagg gcggataaga ttttgactca gctagagaat caaggaacag agagtcctcc
    30781 tcccatgaga caaactagtg ctgtcactga acagatttca ctctttgata gggcagaaga
    30841 gcatcctatc ctagcagaat tagctaaact ggatgtgtat aatatgacac ctatgcaggt
    30901 tatgaatgtc ttagtagagt taaaacagaa actataaaac caagactcac tagttaatct
    30961 agctgtatca aggagacttc tttgacaatt ctccactttt ttgctagaat aacatcacac
    31021 aaacagaatg aaaaggagct gacgcattgt cgctcccttt tgtctatttt taaggagaaa
    31081 gtatgctgat tcagaaaata aaaacctaca agtggcaggc cctggcttcg ctcctgatga
    31141 caggcttgat ggttgctagt tcacttctgc aaccgcgtta tctgcaggaa gtcttaggcg
    31201 ccctccttac tgggaaatat gaagctattt atagtatcgg ggcttggttg attggtgtgg
    31261 ccgtagtcgg tctagttgct ggtggactca atgttgtcct cgcagcctat attgcccaag
    31321 gagtttcatc cgaccttcgg gaggatgcct tccgtaaaat tcaaaccttt tcttatgctg
    31381 atattgaaca atttaatgcg ggaaatctag tcgttcgaat gacaaatgat atcaaccaga
    31441 ttcagaacgt tgtcatgatg accttccaga ttcttttcag acttcccctc ttgttcatcg
    31501 gttcgtttat cctagcggtt caaaccttac cttctctgtg gtgggtgatt gttctcatgg
    31561 tagtcttgat ttttggtttg actgctgtca tgatgggaat gatggggcct cgttttgcca
    31621 agtttcaaac ccttcttgag cgtatcaatg ccattgccaa ggaaaattta cgtggcgttc
    31681 gtgtggtcaa gtcctttgtc caagaaaaag agcaatttgc taagtttaca gaggtctcag
    31741 acgagcttct tggtcaaaac ctttacattg gttatgcctt ttcagtagtg gaacccttta
    31801 tgatgttggt tggttacggg gcggtcttcc tctctatttg gctggtcgcg ggaatggttc
    31861 agtcggatcc gtctgttgtt ggttccatcg cttcttttgt taattaccta agccagatta
    31921 tctttaccat tgttatggtt ggatttttgg gaaattctgt cagccgtgcc atgatttcca
    31981 tgcgtcgtat tcgagaaatt cttgacgcag agccagctat gaccttcaag gatatcccag
    32041 atgaagagtt ggttggaagc cttagctttg aaaatgtgac ctttacctat ccaatggaca
    32101 aggaaccgat gctgaaagat gtgagcttta ctattgaacc tggtcaaatg gttggtgtag
    32161 ttggagcgac tggtgcagga aagtcaactt tggctcaatt gattccacgt ctctttgatc
    32221 cacaggacgg ggccattaaa atcggtggca aggatattcg agaagtgagt gaaggaaccc
    32281 tgcgtaaaac agtttccatc gttctccaac gtgccattct ttttagtgga acgattgcag
    32341 ataacttgag acaggggaag ggaaatgcta ctctatttga aatggagcgc gcagccaata
    32401 ttgcccaggc tagtgaattc attcatcgta tggagaaaac ctttgaaagt ccagttgaag
    32461 aacggggaac caatttctct ggtggacaaa aacaaaggat gtcgattgcg cgtgggattg
    32521 tcagcaatcc acgtattctg atttttgatg attcgacctc agccttggat gccaaatcag
    32581 agcgcttggt gcaagaagct ttgaataagg acttgaaggg tacgacaacc attattattg
    32641 ctcaaaaaat tagctcggtt gtccatgcag acaagatctt ggttctaaat caaggacgat
    32701 tgattggtca aggcacgcat gcagacttgg ttgccaacaa tgccgtttac cgtgaaatct
    32761 atgaaacaca gaaatgaaag acaaactata agaaaagtca atagttttat ctaaactatt
    32821 tcttatttca atttgatgat ttggcgatga ttttagagca cggcaaaaag cccttgaaaa
    32881 agtccatttt ttcaaaggta atcctgtgtt gatttcagaa attacatcac tttttgttcg
    32941 tcaaatggca gctctttttt aggatataaa acagggttcg gataagtttt tttgcaaggt
    33001 ggatgatggc tacattgtaa tgttttcctt attctaactt agtcttaaga taggtcttga
    33061 aagtaggtga aaagcgaggg catgctttgg cagcttgtat gagtacctac cgcagatgag
    33121 gggaaccccg tttgaccatt cttccagcta aatcaatctg acctgactga taaatagaag
    33181 aatccagtcc agcgaaagct tgtaattgag caagattatc aaaggcatga atatttcgaa
    33241 tctcggctaa aatgaccgcc cctaaacgat tcccaatccc agtaaccgtc gtgatgatcg
    33301 agttgaactc agccatcaag tcattgacac atgtttccgc cttgtcaatg agcctcttgt
    33361 aatgtttgat gttttcatta cacgagataa aacgtttatg cgttatcaaa ctcatgacca
    33421 attgaaacaa aaagctgtgg ttagatcctt tcggaaattg tcaagcgatt ggaggaaatg
    33481 aactaatcca cagcggctta ttccaagtat accacttggg ctttggcagt agctaactgc
    33541 gctaaatata atataaggag gagtaaaatg aagacagttc aatttttttg gcattatttt
    33601 aaggtttaca agttctcatt tgtagttgtc atcctgatga ttgttctggc gacttttgcc
    33661 caagccctct ttccagtctt ttctggacaa gcggtgacgc agctagccaa tttagttcaa
    33721 gcttatcaaa atggcaatcc agaacttgta tggcaaagcc tatcaggaat catggtcaat
    33781 cttggcctgc tggttttggt tctatttatc tctagtgtaa tatacatgtg tctcatgacg
    33841 cgcgtgattg cagaatcgac caacgagatg cgcaaaggcc tctttggtaa gcttgctcag
    33901 ttgacggttt ctttctttga ccgtcgacaa gatggcgata tcctgtctca ttttaccagt
    33961 gatttggata atatcctcca agcctttaac gaaagcttga ttcaggtcat gagcaatatt
    34021 gttttataca ttggtctgat tcttgtcatg ttttcgagaa atgtgacgct ggctctcatc
    34081 accattgcca gcaccccatt ggctttcctt atgctgattt tcatcgtgaa aatggcacgc
    34141 aaatacacca acctccagca gaaagaggta gggaagctca acgcctatat ggatgagagc
    34201 atctcaggcc aaaaagccgt gattgtgcaa ggaattcaag aggatatgat ggcaggattt
    34261 cttgaacaaa atgagcgcgt gcgcaaggca acctttaaag gaagaatgtt ctcaggaatt
    34321 cttttccctg tcatgaatgg gatgagcctg attaatacag ccatcgtcat ctttgctggt
    34381 tcggctgtac ttttgaatga taagtctatt gaaacaagta cagccctagg tttgattgtt
    34441 atgtttgcac aattttcaca gcagtactac cagcctatta tccaagttgc agcgagttgg
    34501 ggaagccttc agttggcctt tactggagct gaacgaattc aggaaatgtt tgatgcagag
    34561 gaggaaatcc gacctgaaaa ggctccaacc ttcactaagt tgcaagaaag tgttgaaatc
    34621 cgtcatatcg atttttcata cttgcctgat aaacctattt tgaaagatgt cagcatttct
    34681 gcccctaaag gccagatgac agcagttgtt gggccgacag gttcaggaaa aacgactatt
    34741 atgaacctca tcaatcgctt ttatgatgtt gatgctggtg gtatttattt tgatggtaaa
    34801 gacattcgtg gctatgactt agatagtctt agaagcaagg tgggaattgt attgcaagat
    34861 tcggtcttgt ttagcggaac gattcgagac aatatccgat ttggtgtgcc agatgctagt
    34921 caggaaatgg ttgaggtagc agcaaaagca acccacattc acgactatat cgaaagtttg
    34981 cctgataagt acgatactct tattgatgat gaccagagca tcttttcaac agggcagaag
    35041 caattgattt caatcgctcg aaccctgatg acagatccag aagttctcat tctcgatgaa
    35101 gcaacttcaa acgtagatac ggtgacagaa agcaagattc agcatgccat ggaggtggtt
    35161 gtagcaggta gaactagttt cgtcattgcc caccgcttga aaaccattct caatgcagat
    35221 cagattattg tccttaaaga tggagaagtc attgaacgtg gtaaccacca tgaacttttg
    35281 aagctaggtg gcttttattc agaactctat cacaatcaat ttgttttcga ataagaaaga
    35341 agttgtccta tgtgggcagc tttttcttgt ccataaaaaa tgtttatcac agccttaaaa
    35401 aaaacatatt agacgaaagt cattttgagt gatatgatag gactatcgtt agcattcgaa
    35461 aggagaggca tcatggctag aacggttgta ggagttactg caaatctatg tcccgtagac
    35521 gcagaaggca aaatcattca ttcatctgta tcttgtagat tcgcagagat cattcgtcaa
    35581 gtcggtggtc tccctttagt cattcctgtt ggtgatgagt cagttgtacg tgattatgtg
    35641 gaaatgattg acaaactcat tttgacagga ggccaaaatg ttcatcctca gttttatgga
    35701 gagaaaaaga ccgtcgagag cgatgattac aatctggtcc gtgacgaatt tgaattggca
    35761 ctcttgaagg aagcgcttcg tcagaataaa ccaattatgg caatctgtcg cggtgtccaa
    35821 cttgtcaatg ttgcctttgg tggaaccctc aatcaagaaa tcgaaggtca ctggcaagga
    35881 ctacctttcg ggacatctca ctctattgag acagtagaag gaagcgtggt ggctaagcta
    35941 tttgggaaag aaagtcaggt caattcagtc catcgtcaaa gcattaaaga tttggcaccc
    36001 aatttccgtg taactgctat tgattcaaga gaccaaacta ttgaagcgat tgagtctatc
    36061 gacgagcacc gcattatcgg tttacagtgg catccagagt ttctggttaa tgaagaagat
    36121 ggcaatttag aattatttga gtatttattg aatgaactgt aacgattgga acatctggtc
    36181 gttctttctt ttattttttc aaaattttta gaatgtggta ttttacgcaa acgtttgaat
    36241 tctgataaaa attggagaaa ttcgacaaaa aaacttgaaa aaaacgaagg taagcgttat
    36301 gatagaaaag aagaaatatt ggaggaagaa catgtcacat attaaatttg attattcaaa
    36361 agttttagac aaatttgttg caccacatga agtggaatac atgcaatcac aagtaacagc
    36421 agcagatgaa ttgatccgta aaggaactgg tgctggtagc gacttcttgg gatggttgga
    36481 ccttcctgaa aaatacgacc gcgaagaatt tgaccgcatc ttgaaagctg ctgagcaaat
    36541 caagtcagac agcgatgttt tggttgtgat cggtattggt ggatcttacc ttggtgcaaa
    36601 agcagcaatc gacttcttga atcaccattt tgctaacttg caaacaaaag aagaacgcaa
    36661 agctccacaa atcctttacg caggaaactc aatctcatct acttaccttg ctgacttggt
    36721 agagtatgtt gcagacaaag acttctcagt aaacgtaatt tctaaatcag gtacaacaac
    36781 tgaaccagcg attgctttcc gtgtctttaa agaactcttg gttaagaaat acggtcaaga
    36841 agaagctaac aaacgtatct atgcaacaac tgaccgccaa aagggtgctg ttaaggttga
    36901 agcagacgct aacggttggg aaacatttgt tgttccagat gatatcggtg gacgcttctc
    36961 agtattgaca gccgttggtt tgcttccaat cgcagcatca ggagctgaca tcaaagctct
    37021 tatggaaggt gcgaatgcag ctcgcaaaga ctacacttca gacaaaatct ctgaaaacga
    37081 agcttaccaa tacgcagctg ttcgtaacat cctttatcgt aaaggctatg caactgagat
    37141 cttggtaaac tatgagccat cacttcaata cttctcagaa tggtggaaac aattggctgg
    37201 tgaatcagaa ggaaaagacc aaaaaggtat ctacccaact tcagccaact tctcaactga
    37261 cttgcactca cttggtcaat ttatccaaga aggaactcgt atcatgtttg aaacagttgt
    37321 ccgtgttgac aaacctcgta aaaacgtgct tattcctact ttggaagaag accttgacgg
    37381 acttggttac cttcaaggaa aagacgttga ctttgtaaac aaaaaagcaa ctgacggtgt
    37441 tcttcttgcc cacacagatg gtgatgtacc aaacatgtat gtgactcttc cagagcaaga
    37501 cgctttcact cttggttaca ctatctactt cttcgaattg gcaattgccc tttcaggtta
    37561 cttgaatgct atcaacccat ttgaccaacc aggtgttgaa gcttataaac gtaacatgtt
    37621 tgcccttctt ggaaaaccag gatttgaaga attgagcaaa gaacttaacg cacgtctata
    37681 atagaagaaa agagtggttt acccactctt tttactctct ttatccatag aaattggact
    37741 cagtcaagac ttgtgatata atatagaaag caaaaaggca gatgcctaga taataggaga
    37801 aactatgtca aaagatatcc gcgtacgtta cgcaccaagt ccaacaggac tactacacat
    37861 cggaaatgct cgtacagcat tgtttaatta cttgtatgcg cgccatcatg gtggaacatt
    37921 tctcatccgt atcgaagata ctgaccgtaa acgccatgtc gaggatggtg aacgttcaca
    37981 acttgaaaac cttcgctggt taggcatgga ttgggatgaa agtccagaat cacatgagaa
    38041 ttatcgccag tctgagcgtt tagacttgta tcaaaaatac attgaccaac tattagctga
    38101 aggaaaagcc tataaatctt acgttacaga agaagagttg gcagctgaac gcgaacgcca
    38161 agaagtagct ggcgaaacac cacgctacat caatgaatac cttggtatga gtgaagaaga
    38221 aaaagcagct tacatcgcag aacgtgaagc agcagggatc atcccaactg ttcgtttggc
    38281 tgtcaatgag tcaggtatct acaagtggca tgatatggtc aaaggcgata tcgaatttga
    38341 aggtggcaat atcggtggtg actgggttat ccaaaagaaa gacggttacc caacttacaa
    38401 ctttgccgtt gttatcgatg accacgatat gcaaatctct catgttatcc gtggagatga
    38461 ccatattgct aatacaccaa aacagcttat ggtctatgaa gctcttggtt gggaagctcc
    38521 agagttcggt cacatgacct tgattatcaa ctctgagact ggtaagaagt tatctaaacg
    38581 tgatactaat acacttcaat ttatcgaaga ttaccgaaag aaaggttacc ttccagaagc
    38641 agtctttaac tttattgctc ttcttggttg gaacccaggt ggtgaagatg agattttctc
    38701 tcgtgaagaa ctcattaaac tttttgatga aaaccgcctc agcaagtcac cagcagcctt
    38761 tgatcagaaa aaactagact ggatgagcaa tgattatatc aagaatgcag accttgaaac
    38821 catctttgaa atggcaaaac cattcttaga ggaagcaggc cgtttgactg acaaggctga
    38881 aaaattagtt gagctctata aaccacaaat gaaatcagta gatgagatta tcccattgac
    38941 agatcttttc ttctcagatt tcccagaatt gacagaagca gagcgcgaag tcatgacggg
    39001 tgaaacagtt ccaacagttc ttgaagcatt caaagcaaaa cttgaagcga tgacagatga
    39061 taaatttgtg acagaaaata tcttcccaca aattaaagca gtccaaaaag aaacaggtat
    39121 taaagggaaa aatcttttca tgcctattcg tatcgcagtt tcaggcgaaa tgcatgggcc
    39181 agaattacca gatacaattt tcttgcttgg acgtgaaaaa tcaattcagc atatcgaaaa
    39241 catgctaaaa gaaatctcta aataaagagg atacaataat ggacatctgg gaaaagatgt
    39301 acgaagaagc acagaaacta tacaatccac atgaagtatc agactttgtt tatgccaatc
    39361 atgttgtagc tgcagtagaa gcagaagatg gacaaatatt tacaggattc tgtatggagg
    39421 gaacctgtgg tgttttccat ctctgcgcag aacgggcggc cctcttcaat atgtaccaat
    39481 tttcaggaca aactaaggtt aagaaagtat tagcctttcg agataaacca ccttatggtg
    39541 gaagttcagc catgccttgc ggtgcttgta gaaaattcct tttagagctt aatgctgaaa
    39601 ataaagatgc agagttcatg atggactata atataagaaa aacagttaaa gtcgcagaac
    39661 taatccctta ttggtgggga gaagaacgtg cttctaagtt taatgagcga tagaagagtc
    39721 agtataattc tggctctttt tactataagt tgaaaaaata
//
LOCUS       DAVKJU010000013        38675 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVKJU010000013 DAVKJU010000000
VERSION     DAVKJU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.013"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            344..571
                     /locus_tag="VW191_000575"
     CDS             344..571
                     /locus_tag="VW191_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623390.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            859..3756
                     /locus_tag="VW191_000576"
     CDS             859..3756
                     /locus_tag="VW191_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEU9623391.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDKVVISNLRDNPEAKD
                     PAAPSPLNIDMVLVQDPTTKRIFSIYDMFPEGRAVFGMPKTPEKAYEKIGDKTYQILY
                     KQGESGHYTVRENGEVYNAQNQKTDYRVVVNPTEPGYRDKGNLYKGQELIGNIYFAHS
                     TKNPFRVANTSYLWMSYSDDDGKTWSAPRDITPGLRKDWMKFLGTGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NERAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWDFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            4771..5223
                     /locus_tag="VW191_000577"
     CDS             4771..5223
                     /locus_tag="VW191_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9623392.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFIYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            5242..6579
                     /locus_tag="VW191_000578"
     CDS             5242..6579
                     /locus_tag="VW191_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU9623393.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            6658..7542
                     /locus_tag="VW191_000579"
     CDS             6658..7542
                     /locus_tag="VW191_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9623394.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            7542..8375
                     /locus_tag="VW191_000580"
     CDS             7542..8375
                     /locus_tag="VW191_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9623395.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            8393..10486
                     /gene="nanB"
                     /locus_tag="VW191_000581"
     CDS             8393..10486
                     /gene="nanB"
                     /locus_tag="VW191_000581"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEU9623396.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSTIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     NETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSKVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            10498..11601
                     /locus_tag="VW191_000582"
     CDS             10498..11601
                     /locus_tag="VW191_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9623397.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            11775..12473
                     /locus_tag="VW191_000583"
     CDS             11775..12473
                     /locus_tag="VW191_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEU9623398.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            12492..14009
                     /locus_tag="VW191_000584"
     CDS             12492..14009
                     /locus_tag="VW191_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9623399.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALAVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETIAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            14134..15462
                     /locus_tag="VW191_000585"
     CDS             14134..15462
                     /locus_tag="VW191_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623400.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            15715..16581
                     /locus_tag="VW191_000586"
     CDS             15715..16581
                     /locus_tag="VW191_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9623401.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            16597..17436
                     /locus_tag="VW191_000587"
     CDS             16597..17436
                     /locus_tag="VW191_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9623402.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            17709..18161
                     /locus_tag="VW191_000588"
     CDS             17709..18161
                     /locus_tag="VW191_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEU9623403.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            18303..18611
                     /locus_tag="VW191_000589"
     CDS             18303..18611
                     /locus_tag="VW191_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623404.1"
                     /translation="MDGQGITVDEMAWIIRGIVNALIGRYIKLGTYAAKYGISMARSI
                     LNRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDAHDKIPNNGRINF
                     "
     gene            18895..19539
                     /locus_tag="VW191_000590"
     CDS             18895..19539
                     /locus_tag="VW191_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9623405.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTAPPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            19708..20625
                     /locus_tag="VW191_000591"
     CDS             19708..20625
                     /locus_tag="VW191_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU9623406.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLVGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            20643..21527
                     /locus_tag="VW191_000592"
     CDS             20643..21527
                     /locus_tag="VW191_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9623407.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(21543..22394)
                     /locus_tag="VW191_000593"
     CDS             complement(21543..22394)
                     /locus_tag="VW191_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEU9623408.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            22594..24651
                     /gene="pbp2b"
                     /locus_tag="VW191_000594"
     CDS             22594..24651
                     /gene="pbp2b"
                     /locus_tag="VW191_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEU9623409.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELGNGGAKYSEGVYAVALNPKTGAVLSMSGLKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQPIVFQGSAPIYSWYKLAYGSFPITAVEALEYSSN
                     AYMVQTALGIMGQTYQPNMFVGTSNLETAMGKLRATFGEYGLGAATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANDGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVAGGQEANNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYNQHH
                     PMN"
     gene            24662..25258
                     /gene="recR"
                     /locus_tag="VW191_000595"
     CDS             24662..25258
                     /gene="recR"
                     /locus_tag="VW191_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEU9623410.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSDDDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            25434..26477
                     /locus_tag="VW191_000596"
     CDS             25434..26477
                     /locus_tag="VW191_000596"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU9623411.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSQTPGQEDRLMTNATIDWDKQVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLTYPVFTKPSNMGSSVGISKSENQEELRQALKLAFQYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRRNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPDLIEHLVDL
                     AKESFDKREAHLL"
     gene            26562..27935
                     /locus_tag="VW191_000597"
     CDS             26562..27935
                     /locus_tag="VW191_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU9623412.1"
                     /translation="MKLTIHEVAQAVGAKNNVSIFEDIQLEKVEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTTYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELAHPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMAPGSLLLAP
                     ADSIVEAYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRQALQNLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTDDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLEHEDK"
     gene            27922..28533
                     /locus_tag="VW191_000598"
     CDS             27922..28533
                     /locus_tag="VW191_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9623413.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRGLLSEML
                     NQASDLDAEEVAEVLNPTSAYATPLMDVRAWIVEEEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKFLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIKILWQVYQGQREQYLD"
     gene            28624..29328
                     /locus_tag="VW191_000599"
     CDS             28624..29328
                     /locus_tag="VW191_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEU9623414.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPCPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            29547..30920
                     /gene="ftsA"
                     /locus_tag="VW191_000600"
     CDS             29547..30920
                     /gene="ftsA"
                     /locus_tag="VW191_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU9623415.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            30937..32196
                     /gene="ftsZ"
                     /locus_tag="VW191_000601"
     CDS             30937..32196
                     /gene="ftsZ"
                     /locus_tag="VW191_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU9623416.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVSPQARTATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            32201..32872
                     /locus_tag="VW191_000602"
     CDS             32201..32872
                     /locus_tag="VW191_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU9623417.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            32882..33421
                     /locus_tag="VW191_000603"
     CDS             32882..33421
                     /locus_tag="VW191_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEU9623418.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            33421..33684
                     /locus_tag="VW191_000604"
     CDS             33421..33684
                     /locus_tag="VW191_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU9623419.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            33681..34466
                     /locus_tag="VW191_000605"
                     /pseudo
     CDS             33681..34466
                     /locus_tag="VW191_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            34475..35263
                     /gene="divIVA"
                     /locus_tag="VW191_000606"
     CDS             34475..35263
                     /gene="divIVA"
                     /locus_tag="VW191_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEU9623420.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPVPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            35611..38403
                     /locus_tag="VW191_000607"
     CDS             35611..38403
                     /locus_tag="VW191_000607"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU9623421.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        12037 a         6764 c         8418 g        11456 t
ORIGIN      
        1 gtgttttgag catcatgtgg ctagcttctt agtttgctct ttgattttca ttgagtataa
       61 aaacagatga gtttctgttt tctttttatg gactataaat gttcagctga aactactttc
      121 aaggacatta ttatataaaa gaattttttg aaactaaaat ctactatatt acactatatt
      181 gaaagcgttt taaaaataag gtataataaa tttattaaca cttataaaaa gtgatagaat
      241 ctatctttat gtatatttaa agatagattg ctgtaaaaat agtagtagct atgcgaaata
      301 acagatagag agaagggatt gaagcttaga aaaggggaat aatatgatat ttaaggcatt
      361 caagacaaaa aagcagagaa aaagacaagt tgaactactt ttgacagttt ttttcgacag
      421 ttttctgatt gatttatttc ttcacttatt tgggattgtc ccctttaagc tggataagat
      481 tctgattgtg agcttgatta tatttcccat tatttctaca agtatttatg cttatgaaaa
      541 gctatttgaa aaagtgttcg ataaggattg agcaggaagt atggtgtaaa tagcataagc
      601 tgatgtccat catttgctta taaagagata gtttagttta attgcagcgg tgtcctggta
      661 gataaactag attggcagga gtctgattgg agaaaggaga ggggaaattt ggcaccaatt
      721 tgagatagtt tgtttagttc atttttgtca tttaaatgaa ctgtagtaaa agaaaattaa
      781 taaaagacaa actaagtgca ttttctggaa taaatgtctt atttcagaaa tcgggatata
      841 gatatagaga ggatcagtat gaatcggagt gttcaagaac gtaagtgtcg ttatagcatt
      901 aggaaactat cggtaggagc ggtttctatg attgtaggag cagtggtatt tggaacgtct
      961 cctgttttag ctcaagaagg ggcaagtgag caacctctgg caaatgaaac tcaactttcg
     1021 ggggagagct caaccctaac tgatacagaa aagagccagc cttcttcaga gactgaactt
     1081 tctggcaata agcaagaaca agaaaggaaa gataagcaag aagaaaaaat tccaagagat
     1141 tactatgcac gagatttgga aaatgtcgaa acagtgatag aaaaagaaga tgttgaaacc
     1201 aatgcttcaa atggtcagag agttgattta tcaagtgaac tagataaact aaagaaactt
     1261 gaaaacgcaa cagttcacat ggagtttaag ccagatgcca aggctccagc attctataat
     1321 ctcttttctg tgtcaagtgc tactaaaaaa gatgagtact tcactatggc agtttacaat
     1381 aatactgcta ctctagaggg gcgtggttcg gatgggaaac agttttacaa taattacaac
     1441 gatgcaccct taaaagttaa accaggtcag tggaattctg tgactttcac agttgaaaaa
     1501 ccgacagcag aattgccaaa tggtcgagtt cgcctctacg taaacggggt attatctcga
     1561 acaagtctga aatctggcaa tttcattaaa gatatgccag atgtaacgca tgtgcaaatc
     1621 ggagcaacca agcgtgccaa caatacggtt tgggggtcaa atctacagat tcggaatctc
     1681 actgtgtata atcgtgcttt aacaccagaa gaggtacaaa aacgtagtca actttttaaa
     1741 cgctcagatt tagaaaaaaa actacctgaa ggagcggttt taacagagaa aacggacata
     1801 ttcgaaagcg ggcgtaacgg taaaccaaat aaagatggaa tcaagagtta tcgtattcca
     1861 gcacttctca agacagataa aggaactttg atcgcaggtg cagatgaacg ccgtctccat
     1921 tcgagtgact ggggtgatat cggtatggtc atcagacgta gtgaagataa tggaaagaca
     1981 tggggagata aggtggttat ctccaatctt cgagataatc ctgaagctaa agatcctgct
     2041 gcgccatcgc ctctaaatat tgatatggtt ttggttcaag acccgacaac aaagagaatc
     2101 ttctcaattt atgatatgtt cccagaaggt cgagcagttt ttggaatgcc aaaaacacct
     2161 gaaaaagctt atgaaaagat aggggataaa acttatcaaa tcttgtataa acaaggagag
     2221 tctggtcatt atactgttcg tgagaatgga gaagtgtata atgcacaaaa tcaaaagacg
     2281 gattatcgtg ttgtagtgaa tccaacagaa cctggctata gagataaagg aaatctttac
     2341 aaaggtcagg aattgattgg aaatatctat tttgcacaca gtacaaaaaa tccatttaga
     2401 gtagccaata cgagctatct atggatgtca tatagtgacg atgatggtaa aacttggtct
     2461 gcaccgagag acattactcc aggtcttcgc aaggattgga tgaagttcct aggaacaggt
     2521 cctggaacag gaattgtact tcggaatggg cctcacaagg gacggatttt gataccggtt
     2581 tatacgacta ataatgtatc tcacttaaat ggctcgcaat cttctcgtgt catctattca
     2641 gatgatcatg gaaaaacttg gcatgctgga gaagcggtca acgataaccg tcaggtagac
     2701 ggtcaaaaga tccactcttc tacgatgaac aatgaacgtg cgcaaaatac agaatcaacg
     2761 gtggtacaac taaacaatgg agatgttaaa ctctttatgc gtggtttgac tggagatctt
     2821 caggttgcta caagtaaaga cggaggagtg acttgggaga aggatatcaa acgttatcca
     2881 caggttaaag atgtctatgt tcaaatgtct gctatccata cgatgcacga aggaaaagaa
     2941 tacatcatcc tcagtaatgc aggtggaccg aaacgtgaaa atgggatggt ccacttggca
     3001 cgtgtcgaag aaaatggtga gttgacttgg ctcaaacaca atccaattca aaaaggagag
     3061 tttgcctata attcgctcca agaattagga aatggggagt atggtatctt gtatgaacat
     3121 actgaaaaag gacaaaatgc ctatacccta tcatttagaa aatttaattg ggactttttg
     3181 agcaaagatc tgatttctcc taccgaagcg aaagtgaagc gaactagaga gatgggcaaa
     3241 ggagagatgg gcaaaggagt tattggcttg gagttcgact cagaagtatt ggtcaacaag
     3301 gctccaaccc ttcaattggc aaatggtaaa acagcgactt tcctaaccca gtatgatagc
     3361 aagaccttgt tgtttgcagt agataaggaa gatatcggac aggaaattat tggtatagct
     3421 aaaggaagca tcgaaagtat gcataatctt cctgtaaatc tagcaggtgc cagagttcct
     3481 ggcggagtaa atggtagcaa agcagcggtg catgaagttc cagaatttac agggggagtt
     3541 aatggtacag agccagctgt tcatgaaatc gcagagtata agggatctga ttcgcttgta
     3601 actcttacta caaaagaaga ttatacttac aaagctcctc ttgctcagca ggcacttcct
     3661 gaaacaggaa acaaggagag tgacctccta gcttcactag gactaacagc tttcttcctt
     3721 ggtctgttta cgctagggaa aaagagagaa caataagaga agaattctaa acatttgatt
     3781 ttgtaaaaat agaaggagat agcaggtttt caagcctgct atcttttttt gatgacattc
     3841 aggctgatac gaaatcataa gaggtctgaa actactttca gagtagtctg ttctataaaa
     3901 tatagtagat tgaaataaga tgtgaacaac tctatcagga aagtcaaatt aatttataga
     3961 attattttag cagtcaaggt gtactgttat agattcaata tattatacga ctattaacct
     4021 tgtcttctcc taaaattgac tttcttgttt tcttatcttg tccactcgaa acaagtattg
     4081 taagaatttg attatttttg aaagtacttt taatatactt gatatagtta aaaaagattt
     4141 gaaactaaat tccaaattag aaaaagactt gaaatactaa aaaaaaaaag tatactctaa
     4201 ttgaaaacgg taacaaaact aatttagaga atgaaatata gagtatttct ctcttaaaag
     4261 tttttggtga aacgagatgt agaaaggaga tttagccaaa gagtctatta gtgctagaat
     4321 aatagattag aattatttta gaaaaacgaa gtgagcagct tataaattca agtccccaaa
     4381 tagattcata ctagtatctt ttgcaaaaaa taaagggcga cttccttcat gaatatcaat
     4441 ttcatctata aggaaggtag ctaattgaac taacttattt attctgtttg tcgctagaaa
     4501 aatcagacct ccttgtgaag attgaggaga tactgaatga aaatcaaaga agaaactagc
     4561 aagctagtag cagattgccc aaaacaccgc tttgaggttg tagataagac tgacgtatat
     4621 aatccaaggt gaagcgactg tggtttgaag agattttcaa agagtatagg ctagagagta
     4681 gtgtttttat gtccttctag tagaaaatgc tagacagaag aatggggaac ttggatagga
     4741 aaaatagatt gagaaaggag gttagaagag atgattatta caaaaattag ccgtttagga
     4801 acttatgtgg gagtcaatcc acattttgca acattaatag attttctaga aaaaacagga
     4861 ctagaaaatt taacagaagg ttcgattgct atcgatggta atcgattgtt tgggaattgc
     4921 tttatttatc tagcagatgg tcaagcaggg gctttctttg aaacacacca aaaatatttg
     4981 gatattcatt tagttttgga aaacgaagaa gccatggctg ttacatcgcc ggaaaatgta
     5041 agcgttaccc aagaatatga tgaagagaaa gatattgaat tatacacagg gaaagtggaa
     5101 cagttggttc atttgagagc tggtgaatgc ctcatcactt ttccagaaga tttacatcaa
     5161 cccaaggttc gtataaatga tgaacctgtg aaaaaagttg tctttaaagt tgcgatttct
     5221 taatgtagaa agagaagaac gatgaaaaaa atgagaaagt ttttatgtct agctggaatt
     5281 gcgctagcgg ctgttgcctt ggtagcttgt tcaggaaaaa aagaagctac aactagtact
     5341 gaaccaccaa cagaattatc tggtgaaatt acaatgtggc actcctttac tcaaggaccc
     5401 cgtttagaaa gtattcaaaa atcagcagat gctttcatgc aaaagcatcc aaaaacgaaa
     5461 atcaagattg aaacattttc ttggaatgac ttctatacta aatggactac aggtttagca
     5521 aatggaaatg tgccagatat cagtacagct cttcctaacc aagtaatgga aatggtcaac
     5581 tcagatgctt tggttccgct aaatgattct atcaagcgta ttggacaaga taaatttaac
     5641 gaaactgcct taaatgaagc aaaaatcgga gatgattact actctgttcc tctttattca
     5701 catgcacaag tcatgtgggt tagaacagat ttgttaaaag aacataatat tgaggttcct
     5761 aaaacttggg atcaactcta tgaagcttct aaaaaattga aagaagctgg agtttatggc
     5821 ttgtctgttc cgtttggaac aaatgactta atggcaacac gtttcttgaa cttctacgta
     5881 cgtagtggtg gaggaagcct cttaacaaaa gatcttaaag cagacttgac aagccaactt
     5941 gctcaagatg gtattaaata ctgggttaaa ttgtataaag aaatctcacc tcaagattct
     6001 ttgaatttta atgtccttca acaagctacc ttgttctatc aaggaaaaac agcatttgac
     6061 tttaactctg gcttccatat cggaggaatt aatgccaaca gtcctcaatt gattgattcg
     6121 attgatgctt atcctattcc aaaaatcaaa gagtctgata aagaccaagg aattgaaacc
     6181 tcaaacattc caatggttgt ttggaaaaat tcaaaacatc cagaagttgc taaagcattc
     6241 ttagaagcac tttataatga agaagactac gttaaattcc ttgattcaac tccagtaggt
     6301 atgttgccaa ctattaaggg gattagcgat tctgcagcct ataaagaaaa tgaaactcgt
     6361 aagaagttta aacatgctga agaagtaatt actgaagctg ttaaaaaagg tactgctatt
     6421 ggttatgaaa atggaccaag tgtacaagct ggtatgttga ctaaccaaca cattattgaa
     6481 caaatgttcc aagatatcat tacaaatgga acagatccta tgaaagcagc aaaagaagca
     6541 gaaaaacaat taaacgattt atttgaggct gttcagtaga tgtaaaagac tagaaaatag
     6601 gtgggctagt gatctgaaaa gctctagccc aattttgtaa aagaagggag aaggagaatg
     6661 gttaaagaac gtaatttaac tcgctggata tttgttttgc cagctatgat tatcgtagga
     6721 ttactctttg tttatccgtt tttctcgagt attttttata gctttaccaa taagcatttg
     6781 attatgccta attataaatt tgttggtttg gctaactata aagctgtgct atcagatccc
     6841 aacttcttta atgcgttctt taattcaatt aagtggaccg ttttctcatt agttggtcaa
     6901 gttttagtag ggtttgtatt ggctttagct cttcacagag tacgccactt caagaaatta
     6961 tataggacct tattgattgt tccttgggca tttcctacca tcgttattgc cttctcttgg
     7021 cagtggattc taaacggggt ttatggctac ttacctaatc taatcgtaaa attaggttta
     7081 atggaacata cacctgcatt tttgacagat agtacatggg cattcctatg tttggtgttt
     7141 atcaacattt ggtttggagc accaatgatt atggttaatg tgctttcagc tttgcaaaca
     7201 gtaccagaag aacaatttga ggctgctaag atagatggtg cttcaagttg gcaggtgttc
     7261 aagtttatcg tctttccaca tattaaagtg gttgtaggac ttctagttgt tttgagaact
     7321 gtatggatct ttaataactt tgacattatc tacctcatta ctggtggtgg accagccaat
     7381 gctacaacga cgcttccaat ttttgcttac aacctgggct ggggaactaa attgttgggt
     7441 cgtgcttcag cagttacagt actgctcttt atcttcttgg tggcgatttg ctttatctac
     7501 tttgctatca tcagtaagtg ggaaaaggag ggtagaaaat aatgaagaag aaatccagta
     7561 tttatttaga tattctctca catgtacttt tagttggtgc gaccatcgtt gcagttttcc
     7621 cattggtatg gattatcata tcttctgtca aagggaaagg ggaattaact cagtatccaa
     7681 cacgattttg gcctgaacag tttacattag attatttcac tcatgttatc aacgatttgc
     7741 acttcattga taacattcga aacagtttaa tcattgcctt ggctacaacc cttattgcga
     7801 ttattatttc tgctatggca gcctatggta ttgttcgatt ctttcctaaa ttgggagcaa
     7861 tcatgtcgag actactcgtc attacctaca ttttcccacc aattttgtta gcaattccct
     7921 attcaattgc cattgctaaa gttgggttaa caaatagttt atttggcttg atgatggttt
     7981 atctatcttt tagtgttcca tatgcagttt ggctcttagt tggatttttc caaacagttc
     8041 caattggaat tgaagaagcg gctagaattg atggtgcaaa taaatttgtt acgttttata
     8101 aagttgtgct accgattgta gcaccaggta ttgtagcaac agctatttat acatttatca
     8161 atgcttggaa tgaattcctg tatgccttga ttttgattaa caatacagga aagatgacag
     8221 tagcagtagc ccttcgttca cttaatggtt cagaaatact agactgggga gatatgatgg
     8281 cagcgtctgt tattgtagtt cttccatcaa ttattttctt ctctatcatc caaaataaga
     8341 ttgcaagtgg attatcagaa ggatctgtga agtagacgaa agaaggaaaa aaatgaataa
     8401 aagaggtctt tattcaaaac taggaatttc cgttgtaggc attagtcttt taatgggagt
     8461 ccccactttg attcatgcga atgaattaaa ctatggtcaa ctgtccatat ctcctatttt
     8521 tcaaggaggt tcatatcaac tgaacaataa gagtatagat atcagctctt tgttattaga
     8581 taaattgtct ggagagagtc agacagtagt aatgaaattt aaagcagata aaccaaactc
     8641 tcttcaagct ttgtttggcc tatctaatag taaagcaggc tttaaaaata attacttttc
     8701 aattttcatg agagattctg gtgagatagg tgtagaaata agagacgccc aaaagggaat
     8761 aaattattta ttttccagac cagcttcatt atggggaaaa cataaaggac aggcagttga
     8821 aaatacacta gtatttgtat ctgattctaa agataaaaca tacacaatgt atgttaatgg
     8881 aatagaagtg ttctctgaaa cagttgatac atttttgcca atttcaaata taaatggtat
     8941 agataaggca acactaggag ctgttaatcg tgaaggtaag gaacattacc tcgcaaaagg
     9001 aagtattgat gaaatcagtc tatttaacaa agcaattagt gatcaggaag tttcaactat
     9061 tcccttgtca aatccatttc agttaatttt ccaatcagga gattctactc aagctaacta
     9121 ttttagaata ccgacactat atacattaag tagtggaaga gttctatcaa gtattgatgc
     9181 acgttatggt gggactcatg attctaaaag taagattaat attgccactt cttatagtga
     9241 tgataatggg aaaacgtgga gtgagccaat ttttgctatg aagtttaatg actatgagga
     9301 gcagttagtt tactggccac gagataataa attaaagaat agtcaaatta gtggaagtgc
     9361 ttcattcata gattcatcca ttgttgaaga taaaaaatct gggaaaacga tattactagc
     9421 tgatgttatg cctgcgggta ttggaaataa taatgcaaat aaagccgact caggttttaa
     9481 agaaataaat ggtcattatt atttaaaact aaagaagaat ggagataacg atttccgtta
     9541 tacagttaga gaaaatggtg tcgtttataa tgaaacaact aataaaccta caaattatac
     9601 tataaatgat aagtatgaag ttttggaggg aggaaagtct ttaacagtcg aacaatattc
     9661 ggttgatttt gatagtggct ctttaagaga aaggcataat ggaaaacagg ttcctatgaa
     9721 tgttttctac aaagattcgt tatttaaagt gactcctact aattatatag caatgacaac
     9781 tagtcagaat agaggagaga gttgggaaca atttaagttg ttgcctccgt tcttaggaga
     9841 aaaacataat ggaacttact tatgtcccgg acaaggttta gcattaaaat caagtaacag
     9901 attgattttt gcaacatata ctagtggaga actaacctat ctcatttctg atgatagtgg
     9961 tcaaacatgg aagaaatcct cagcttcaat tccgtttaaa aatgcaacag cagaagcaca
    10021 aatggttgaa ctgagagatg gtgtgattag aacattcttt agaaccacta caggtaagat
    10081 agcttatatg actagtagag attctggaga aacatggtcg aaagtttcgt atattgatgg
    10141 aatccaacaa acttcatatg gcacacaagt atctgcaatt aaatactctc aattaattga
    10201 tggaaaagaa gcagtcattt tgagtacacc aaattctaga agtggccgta agggaggtca
    10261 attagttgtc ggtttagtca ataaagaaga tgatagtatt gattggaaat accactatga
    10321 tattgatttg ccttcgtatg gttatgccta ttctgcgatt acagaattgc caaatcatca
    10381 cataggtgta ctgtttgaaa aatatgattc gtggtcgaga aatgaattgc atttaagcaa
    10441 tgtagttcag tatatagatt tggaaattaa tgatttaaca aaataaagga gaaaaacatg
    10501 gttaaatacg gtgttgttgg agcagggtat tttggagctg aattggctcg ctacatgcaa
    10561 aagaatgatg gagcagagat tactcttctc tatgatccag ataatgcaga ggcgattgca
    10621 gaagaattgg gggcaaaagt agcaagttcc ttagatgagt tggtttctag cgatgaagta
    10681 gattgtgtta tcgtcgcaac tccaaataat cttcataagg aaccggttat taaggctgca
    10741 cagcatggta aaaatgtttt ctgtgaaaaa ccaattgcgc tttcttatca agattgtcgc
    10801 gagatggtag atgcgtgtaa agaaaacaat gtaaccttta tggcaggaca tattatgaat
    10861 ttctttaatg gtgttcatca tgcaaaagaa ctcattaatc aaggagttat cggagacgtt
    10921 ctatattgtc atacagctcg taatggttgg gaagaacaac aaccgtcagt atcatggaaa
    10981 aaaattcgtg aaaaatcagg tggtcacttg tatcaccaca tccatgaatt ggattgcgtt
    11041 caattcctta tggggggcat gcctgaaact gtaaccatga caggtggaaa tgtggcccat
    11101 gaaggtgaac atttcggtga tgaagatgat atgatttttg tcaatatgga attttctaat
    11161 aagcgttttg ctttgttaga atggggttca gcttatcgtt ggggtgaaca ttatgtctta
    11221 atccaaggaa gcaaaggtgc catccgctta gacttattca actgtaaagg aactcttaag
    11281 ctagatgggc aagaaagcta tttcttgatt cacgaatcgc aagaagaaga tgatgatcgg
    11341 actcgtatct atcatagtac agagatggat ggagcaattg cttatggtaa accaggtaaa
    11401 cgtactccat tatggctatc atctgtcatt gataaagaaa tgcgctatct gcatgagatt
    11461 atggaaggag ctccagtatc agaagaattt gcaaaacttt tgacaggtga agctgcccta
    11521 gaagcaattg ctactgcaga tgcttgtacc cagtctatgt ttgaagatcg caaagtaaaa
    11581 ttgtcagaaa ttgtaaaata aattttggta ttctcctatt tataggtcga cttgctcctc
    11641 tgaaagtact tttagaggag ctgtttgact ttgctagttt ttgaaactga aatctattat
    11701 actacaaact attgaaagcg ttttaatttt aaggtataat aatctcatag aaataaagaa
    11761 aaggaggaaa gaggatgcca cagattagca aagaagcctt gattgagcaa atcaaagatg
    11821 gaatcatcgt ttcttgtcag gctcttcctc atgaaccgct ttatacagaa gcgggagggg
    11881 tgattccctt gctggtcaaa gcggctgagc aaggtggagc agtcggtatc cgagcaaaca
    11941 gtgttcgcga tatcaaggaa attaaggaag tcactaaact tccaatcatt gggattatca
    12001 aacgtgatta tccacctcag gaacccttca tcacggctac tatgaaagaa gttgatgaat
    12061 tggcagaact ggacatcgag gtgattgctc tggattgtac caagcgtgaa cgctacgatg
    12121 gtttggaaat tcaagagttc attcgtcagg ttaaggagaa atatcctaat cagcttttga
    12181 tggctgatac tagtatcttc gaagaagggc tagcagctgt agaagcagga attgactttg
    12241 tcggaacaac cttatcaggc tacacatcct acagtccaaa agtagacggt ccagattttg
    12301 aattgattaa gaaactctgt gatgctggtg tagatgtcat tgcagaagga aaaattcata
    12361 caccagaaca agccaaacaa atccttgaat atggagtgcg aggcatcgtt gttggtggcg
    12421 ccattactag accaaaagag attacagaac gcttcgttgc tagtcttaaa taagatgtga
    12481 gggggagttt tatgtttaaa gttttacaaa aagttggaaa agcttttatg ttacctatag
    12541 ctatacttcc tgcagcaggt ctacttttgg ggattggtgg tgcactttca aacccaacca
    12601 cgatagcaac ttatccaata ctagacaata gtatttttca atcgatattc caagtaatga
    12661 gctctgcagg agaggttgta ttcagtaatt tgtcactact tctctgtgtg ggattatgta
    12721 ttggcttagc gaaacgagat aaaggaaccg ctgcgttagc aggagtaact ggttacttag
    12781 ttatgactgc aacgatcaaa gctttggtaa aactttttat ggcagaagga tctgcaattg
    12841 atactggagt tattggagca ttagctgtcg gaatagttgc cgtatatttg cacaaccgat
    12901 ataacaatat tcaattacct tccgctttag gattctttgg aggttcacgc ttcgttccta
    12961 ttgttacatc gttctcttct atcttgattg gctttgtctt ctttgttatt tggccacctt
    13021 tccaacaact tcttgtttct acaggtggat atatttctca ggcgggtcca attggaactt
    13081 ttctatatgg atttttaatg agactttctg gagcagtagg cttacatcat ataatttacc
    13141 ctatgttttg gtatactgaa cttggtggtg ttgaaactat tgcaggacaa acagtggttg
    13201 gagctcaaaa aatatttttt gctcaattag ccgatccggc ccattctgga ttatttacag
    13261 aaggaacaag gttttttgca ggtcgtttct caacaatgat gttcggttta ccggctgcct
    13321 gtttagcgat gtaccatagt gttcctaaaa atcgtcgtaa aaaatacgcg ggtttgtttt
    13381 ttggagttgc tttaacatct tttattaccg gtattacaga accaattgaa tttatgtttc
    13441 tattcgtcag tccggttcta tatgttgttc acgcattcct tgatggtgtt agcttcttta
    13501 ttgcagacgt cttaaatatt tcaataggaa acacattttc aggaggtgta atcgatttca
    13561 ctttatttgg aattttgcag gggaacgcta agacgaattg ggttcttcag attccaattg
    13621 gacttatttg gagtgttttg tattatatta tttttagatg gttcattact caattcaacg
    13681 ttctaacgcc aggacgagga gaagaagtag attctaaaga aatttctgaa tccgcagatt
    13741 caacttcaaa tactgcagat tatttaaaac aggatagcct acaaattatc agagcgttgg
    13801 gtggatcaaa taatatagaa gatgtagatg cttgtgtgac acgtttacgt gtagctgtaa
    13861 aagaagttaa tcaagttgat aaagcacttt taaaacaaat tggtgcagtt gatgtcttag
    13921 aagtgaaggg tggcattcaa gcaatctatg gagcaaaagc aatcttatat aaaaatagta
    13981 ttaatgaaat tttaggtgta gatgattaag tacttactga cttaataaaa aacagaggag
    14041 agtgatggat gagtaggatg aaatgaaatc gcatacaaga aataaagaac tcattatcca
    14101 agttggatac gcttattaca taggagaata caaatgaaat ttagaaaatt agcttgtaca
    14161 gtacttgcgg gtgctgcggt tcttggtctt gctgcttgtg gcaattctgg cggaagtaaa
    14221 gatgctgcca aatcaggtgg tgacggtgcc aaaacagaaa tcacttggtg ggcattccca
    14281 gtatttaccc aagaaaaaac tggtgacggt gttggaactt atgaaaaatc aatcatcgaa
    14341 gcgtttgaaa aagcaaaccc agatataaaa gtgaaattgg aaaccatcga cttcaagtca
    14401 ggtcctgaaa aaatcacaac agccatcgaa gcaggaacag ctccagacgt actctttgat
    14461 gcaccaggac gtatcatcca atacggtaaa aacggtaaat tggctgagtt gaacgacctc
    14521 ttcacagatg aatttgttaa agatgtcaac aatgaaaaca tcgtacaagc aagtaaagct
    14581 ggagacaagg cttatatgta tccgattagt tctgccccat tctacatggc aatgaacaag
    14641 aaaatgttag aagatgctgg agtagcaaac cttgtaaaag aaggttggac aactgatgat
    14701 tttgaaaaag tattgaaagc acttaaagac aagggttaca caccaggttc attgttcagt
    14761 tctggtcaag ggggagacca aggaacacgt gcctttatct ctaaccttta tagcggttct
    14821 gtaacagatg aaaaagttag caaatataca actgatgatc ctaaattcgt caaaggtctt
    14881 gaaaaagcaa ctagctggat taaagacaat ttgatcaata atggttcaca atttgacggt
    14941 ggggcagata tccaaaactt tgccaacggt caaacatctt acacaatcct ttgggcacca
    15001 gctcaaaatg gtatccaagc taaactttta gaagcaagta aggtagaagt ggtagaagta
    15061 ccattcccat cagacgaagg taagccagct cttgaatatc ttgtaaacgg gtttgcagta
    15121 ttcaacaata aagacgacaa gaaagtcgct gcatctaaga aattcatcca gtttatcgca
    15181 gatgacaagg agtggggacc taaagacgta gttcgtacag gtgctttccc agtccgtact
    15241 tcatttggaa aactttatga agacaaacgc atggaaacaa tcagtggctg gactcaatac
    15301 tactcaccat actacaacac tattgatgga tttgctgaaa tgagaacact ttggttccca
    15361 atgttgcaat ctgtatcaaa tggtgacgaa aaaccagcgg atgctttgaa agccttcact
    15421 gaaaaagcaa acgaaacaat caaaaaagct atgaaacaat agtccttagt tattctatga
    15481 aaaagtaatt ttttaaagaa cctaagagtg tataccccct tttccctcta cacagatagt
    15541 gtaagaaaag ggggcttttg tttaaaatgt aagaaactgt cacgaaatta aaatgaagtt
    15601 cttacataag cgaaccataa aaaatttcat tttgatttta aaacagttca agaaagtcaa
    15661 aaaattattc tatttgaaag agaggtgccg actgtgaaag tcaataaaat ccgtatgcgg
    15721 gaaacagtga tttcctacgc tttcctagca ccagtattat tcttctttgt catatttgtg
    15781 ttggctccga tggtgatggg cttcattaca agtttcttta actactcaat gactaaattt
    15841 gagtttgtag gcttggataa ctatatccgt atgtttaaag atcctgtctt tacaaaatct
    15901 ctgattaaca cagttatttt ggttattgga tctgtaccag ttgttgttct attctcactc
    15961 tttgtagcat ctcagaccta tcatcaaaat gtcattgcca gatccttcta ccgtttcgtc
    16021 ttcttccttc ctgttgtaac gggtagtgtt gccgtgacag ttgtttggaa atggatttat
    16081 gacccactat cagggattct aaactttgtc cttaagtcca gccacatcat cagccaaaac
    16141 atttcttggt tgggagataa aaactgggca ttgatggcga ttatgattat tctcttgacc
    16201 acttcagttg gtcagcccat catcctttat atcgctgcca tggggaatat tgacaattca
    16261 ctggttgaag cggcgcgtgt tgatggtgca actgagtttc aagttttttg gaagattaaa
    16321 tggccaagcc ttcttccaac aactctttat attgcaatca tcacaacaat taactcattc
    16381 cagtgtttcg ccttgattca gcttttgaca tctggtggtc caaactactc aacaagtacc
    16441 ttgatgtact acctttacga aaaagccttc caattgacag aatacggcta tgccaacaca
    16501 attggtgtct tcttggcagt catgattgct atcgtaagct ttgttcaatt taaagtactt
    16561 ggaaacgacg tagaatacta aagaaaggag acagctatgc aatctacaga aaaaaaacca
    16621 ttaacagcct ttactgttat ttcaacaatc attttgctct tgttgactgt gctgttcatc
    16681 tttccattct actggatttt gacaggggca ttcaaatcac aacctgatac aattgttatt
    16741 cctcctcagt ggttccctaa aatgccaacc atggaaaact tccaacaact catggtgcag
    16801 aaccctgcct tgcaatggat gtggaactca gtatttatct cattggtaac catgttctta
    16861 gtttgtgcaa cctcatctct agcaggttat gtattggcta aaaaacgttt ctatggtcaa
    16921 cgcattctat ttgctatctt tatcgctgct atggcgcttc caaaacaagt tgtccttgta
    16981 ccattggtac gtatcgtcaa cttcatggga atccacgata ctctctgggc agttatcttg
    17041 cctttgattg gatggccatt cggtgtcttc ctcatgaaac agttcagtga aaatatccct
    17101 acagagttgc ttgaatcagc taaaatcgac ggttgtggtg agattcgtac cttctggagt
    17161 gtagccttcc cgattgtgaa accagggttt gcagcccttg caatctttac cttcatcaat
    17221 acttggaatg actacttcat gcagttggta atgttgactt cacgtaacaa tttgaccatc
    17281 tcacttgggg ttgcgaccat gcaggctgaa atggcaacca actatggttt gattatggca
    17341 ggagctgccc ttgctgctgt tccaatcgtc acagtcttcc tagtcttcca aaaatccttc
    17401 acacagggta ttactatggg agcggtcaaa ggataatact ctgcgaaaat cgaatgcaaa
    17461 ctacgtcagc ttcaccttgc catacttaag tattgcctgt ggttagcttc ctagtttgtt
    17521 cttcaatttt cattgagtat aggaaaatca atctatcaaa atacagaagt atattttata
    17581 gatttagaga atatagaagt tataagtgtc tacaaaatgg agggtatgca gttactttat
    17641 gaagttttgt cagacactta tcaacttaag aatggtttta gttaactatc agaaacgaag
    17701 gaaagagtat gatttttgac gatttgaaaa acatcacctt ttacaaaggg attcatccca
    17761 atttagacaa ggctatcgac tatctctacc aacatcgtaa agattcattc gaattaggaa
    17821 agtatgagat tgatggagat aaagtctttc tagttgttca ggaaaatgtc ctcaatcaag
    17881 ttgagaataa tcaatttgaa caccataaga actatgcaga tttgcatttg ctggtagaag
    17941 gacatgaata ttcgagctac ggttcacgta tcaaagacga ggcagtagca ttcgacgaag
    18001 cgagtgacat tggctttgtt cattgtcatg aacactaccc actcttgttg ggttatcaca
    18061 attttgcgat tttcttccca ggtgagccac atcagccaaa tggttatgca ggcatggaag
    18121 aaaaggttcg aaaatatctc tttaaaattt tgattgatta aaaataggat gaattgtttt
    18181 tttgtaaagc tttgacaata ctctaccatg aaattgatct ttgtgaggta gggaaatgag
    18241 aataaaatat ttaaaaattg gtatcttctt gtcagtctgt gtatgctaca agagctagtt
    18301 tcttggatgg acagggaatt acagttgatg agatggcttg gataattagg ggcattgtga
    18361 atgcattgat tggtagatac ataaaattag gtacttatgc ggctaagtat ggtattagta
    18421 tggcgcgttc tatcttaaat agggcagctg caactgcggc agcaagagta ggattattga
    18481 ctaagatttt tggatggatt ttacgaatag ctgtgctcgc agctgatgta tatggtaatt
    18541 ttgccaacaa tattgctgta gcttgggatg cacatgataa aatccctaac aatggtcgta
    18601 taaactttta aaatgcgaga atgaaagcac tttgtatttt tttattgaat atgttagctt
    18661 ggacagtgct tgcaatgata atccgtggag ggctagatgg atttgatagg catacttgga
    18721 gtactatttt aattgcttcg ctgttcgggg tatatgatta taagcccata gataaaaata
    18781 gaaaaaagtc caaaagaaaa aagaaaaaat agatttgttc atggtaggga cttatgaaag
    18841 ctttactgac aaaaaagaaa acagtttaca aagaaaaatg atggaggagc aaacatggca
    18901 caaaaaggag taagccttat caaggcagca tttgatacag acaactttct catgcgtttt
    18961 agtgagaaag tcttggacat cgtgacagcc aatcttcttt ttgtcgtctc ttgtttgccc
    19021 atcgtgacga ttggagtggc taaaatcagc ctctacgaga ccatgttcga agttaagaag
    19081 agcagacggg tgcctgtttt taaaatctat ctaagatctt tcaagcaaaa tctgaaacta
    19141 ggtcttcagc tgggtttaat ggagttagga attgtgtttc ttaccctttc agatctctat
    19201 cttttctggg gtcaaacagc tccgcccttc caattgctga aagccatttg tttaggtatt
    19261 ctgatttttc ttactatcgt gatgctggct agttacccta tcgcggcacg ttatgaccta
    19321 tcttggaaag aaattcttca aaaaggattg atgttggcta gttttaactt tccttggttc
    19381 ttcctcatgt tagccattct tgtcctcatt gtgatggttc tttatctgtc cgccttcagt
    19441 ctactcttag gtggctcagt cttcttactt tttgggtttg gactattggt ctttatccag
    19501 actggattga tggagaaaat tttcgcaaaa taccaatagg agccttattt ctgaaactac
    19561 tttcaaacgc tccaaacgct attctataag cgagaaacta aaatctaagt gtctataaga
    19621 tattgaaagc cttttcggta aagtgtatac taaagaagta aagaaaaatc tgctttagca
    19681 gaaaataaaa ataataggag aaaatctatg tcagatttga aaaaatacga aggtgtcatt
    19741 ccagccttct acgcatgtta tgatgatcaa ggagaagtaa gcccagaacg tacgcgtgcc
    19801 ttggttcaat acttcattga taaaggtgtt caaggtcttt atgtcaatgg ttcttctggt
    19861 gaatgtatct accaaagcgt tgaagatcgc aagttgattt tggaagaagt catggcggta
    19921 gccaaaggta aattgaccat cattgctcat gttgcctgca acaatactaa agatagtatg
    19981 gaacttgctc gccatgctga aagcttggga gtagatgcta ttgcaacgat tccaccaatt
    20041 tatttccgct tgccagaata ctcagttgcc aaatactgga acgatatcag ttctgcagct
    20101 ccaaacacag actacgtgat ttacaacatt cctcaattgg caggggttgc tttgactcca
    20161 agcctttaca cagaaatgtt gaaaaatcct cgtgttatcg gtgttaagaa ctcttctatg
    20221 ccagttcaag atatccaaac ctttgtcagc cttggtgggg aagaccatat cgtctttaat
    20281 ggtcctgatg agcagttcct aggaggacgc ctcgtgggtg ctagggctgg tatcggtggt
    20341 acttatggtg ctatgccaga actcttcttg aaactcaatc agttgattgc ggataaggac
    20401 ctagaaacag cgcgtgaatt gcagtatgct atcaacgcaa tcattggtaa actcacttct
    20461 gctcatggaa atatgtacgg tgtcattaaa gaagtcttga aaatcaatga aggcttgaat
    20521 attggatctg ttcgttcacc attgacacca gtgactgaag aagatcgtcc agttgtagaa
    20581 gcggctgctg ccttgattcg tgaaaccaag gagcgcttcc tctaatctaa aaggaggtat
    20641 ttatgacata ctacgttgca attgatatcg gtggaaccaa catcaagtat ggtttggttg
    20701 atcaagaggg gcaacttctt gaatcgcatg aaatgccaac tgaggcgcat aagggtggac
    20761 ctcatatctt acaaaagacc aaagatatcg tagctagtta tttagaaaaa ggcccagtag
    20821 caggtgttgc catatcttct gctgggatgg tggatccgga taagggtgag attttctatg
    20881 ctgggcctca aatccctaac tacgcaggca cccagttcaa aaaggaaatc gaagaaagct
    20941 ttactattcc ttgtgagatt gaaaatgatg tcaactgtgc aggtcttgct gaggcagtat
    21001 ctggttcagg caagggagca agtgtgacac tttgcttgac cattggaacc ggtatcggtg
    21061 gttgcttgat tatggatagg aaagtcttcc atggttttag caattcagcc tgtgaagtcg
    21121 ggtatatgca tatgcaggat ggagcttttc aggatctcgc ttctacaaca gctttagtga
    21181 aatatgtagc tgaagcccat ggagaagatg ttgatcagtg gaatggccga cgtatcttta
    21241 aggaagctac tgaaggaaac aaaatctgca tggaaggtat tgaccgtatg gttgactatc
    21301 taggaaaagg tctggcaaat atttgttatg tggccaatcc agaagtggtt attcttggtg
    21361 gtggtatcat ggggcaagag gctatcctca aacctaagat ccgcacagcc ttgaaagagg
    21421 cattggtacc aagtttagca gaaaaaacac gattagaatt tgcccatcac caaaatacag
    21481 cagggatgtt gggtgcatat tatcatttta agacaaaaca atcctagttt ggctcagcca
    21541 aactaggatt ttcttacacg tttttgtcta cgatagccgt tgagtttttt attttcccag
    21601 tagctattaa agattttttc cttgctttcg cgattgattt ccaaaaagta ggcataaatc
    21661 aaatcgataa agaagagcat aggaagttga gcggatattc gttggatgta ggagggttgg
    21721 ctgtgggtgg ctacaagaac agtctctgta taggtctggc tatctttatt gggaacactt
    21781 gtaaagagta cagtctttgc ccccatctcc ttggcatcta atagactatc taaaatagaa
    21841 ggagttgagc ctgaaagtga gaaccccagt actagacaat tttcatccat gatgctggtt
    21901 gtccaggcaa agccgtcttg gtctgtcaaa gcttcgcaga ccacacctag tcgcataaaa
    21961 cgtaatttca tttcacgggc gacgaggcca gaactccctg ttccaaagaa gtagatacgc
    22021 tcagcatctt cgattagctg ggcaattcgc tctagttgga tttcgtcaat caagtcttgt
    22081 gtttgttccc tcatattgct ataacttctg aggactcgtt tggtcagtgg actatgcttg
    22141 gagacttggt tggcttgatt ttctgcctga tgttggtatt ggaaaataaa ttctcggtag
    22201 ccagtaaagc cacacttttt agcaaagcgg gtcaaagcag cttgagaaat atgtaatttt
    22261 tgggtgactt gttgagaaga taaatcatct gtaatcgttt cagcttgcaa aaaatagcga
    22321 gcgatttctt gctctaggtc tgtcatttct tcaaaatgtg aatcaatgat agttgcgata
    22381 tctggtttgt ccatagggaa agctccttta catgggtcat actggaagac tagatcaggg
    22441 aatagtcaca cttcattata acacataata taaggataga taaataaaaa cgcatctctg
    22501 ttttaaaaac gaaaaaatcg aaaaagcttc tctcttttcc ataattttct actcaaattg
    22561 tggtacaatt aagagtaaga ttttaagtta gaaatgagac tgatttgtat gagaaaattt
    22621 aacagccatt cgattccgat tcggcttaat ttattgtttt caatcgtcat tttactcttt
    22681 atgaccatta ttggtcgttt gttgtatatg caggttttga acaaggattt ttacgaaaaa
    22741 aagctagcct cagctagtca gaccaagatt acaagcagtt cagcccgtgg ggaaatttat
    22801 gatgctagtg gaaaaccttt ggtagaaaat acgttaaagc aggttgtttc ctttacgcgt
    22861 agcaataaaa tgacggctac agacttaaaa gaaacagcta aaaagttact gacttatgtg
    22921 agcatcagtt ctccaaattt gacagaacgc cagctggcgg attactattt ggctgatcct
    22981 gaaatctata aaaaaacagt ggaagctctc ccaagtgaga aacgcttgga ttcagatggc
    23041 aatcgtctat ccgaatcaga actgtataac aatgcggtcg atagtgtcca aacaagtcaa
    23101 ctaaactata cagaggatga aaagaaagaa atctatcttt ttagtcagtt aaatgctgtt
    23161 ggaaactttg cgacaggaac cattgcgaca gatcctctaa atgattctca ggtggctgtt
    23221 attgcctcta tttcaaagga gatgcctggc attagtattt ctacttcttg ggatagaaag
    23281 gttttggaaa cttccctttc ttctatagtt gggagtgtat ccagtgaaaa agctggtctc
    23341 ccagcggaag aagcagaagc ctatcttaaa aaaggctatt ctctaaatga ccgtgttgga
    23401 acctcgtatt tggaaaagca atatgaagag accttacaag gaaaacgctc ggtaaaagaa
    23461 atccatctgg ataaatatgg caatatggaa agcgtggata caattgagga aggtagtaag
    23521 ggaaacaata tcaagctgac cattgatttg gccttccaag atagcgtgga tgctttgctg
    23581 aaaagttatt tcaattccga gctaggaaat ggtggagcca agtattctga gggtgtgtat
    23641 gcagtcgccc ttaaccccaa aacaggtgct gttttgtcta tgtcaggact caaacatgac
    23701 ctgaaaacgg gagagttgac tcctgattcc ttgggaacgg taaccaatgt ctttgtccca
    23761 ggttcggttg ttaaggccgc taccatcagc tcaggttggg aaaatggtgt tttatcagga
    23821 aaccaaacct taacagatca gcctattgtt ttccaaggtt cagctccaat ttattcttgg
    23881 tataaattgg catatggatc ttttcctatt acagctgtgg aagccttgga gtattcatcc
    23941 aatgcttaca tggttcaaac cgctcttgga atcatgggcc agacctatca accaaatatg
    24001 tttgttggaa ccagcaattt ggaaacagct atgggaaaac ttcgtgcgac ctttggcgaa
    24061 tatggcttgg gggctgcgac cggaattgac ctaccagatg aatctactgg atttgttccc
    24121 aaagagtata gctttgctaa ttacatcacc aatgcctttg ggcagtttga taactatacg
    24181 cccatgcagt tggctcagta tgtagcaact attgcaaatg atggtgttcg tgtggctcct
    24241 cgtattgttg aaggcattta tggtaataat gataagggag gactgggtga cttgattcag
    24301 caactgcaac cgacagagat gaataaggtc aatatatccg actccgatat gagtatcttg
    24361 caccaaggtt tttatcaggt tgctcatggg actagtggat tgacaactgg acgtgctttt
    24421 tcaaatggtg ccttggtatc cattagtggg aaaacaggta ctgccgaaag ttatgttgca
    24481 ggtggtcaag aagccaacaa taccaacgcg gtggcctatg ccccatcaga taatcctcaa
    24541 atcgctgttg ccgttgtctt ccctcataac accaatctaa caaatggtgt tggaccttct
    24601 attgcacgcg atattatcaa cctctataac caacaccatc caatgaatta gaaaggaact
    24661 tatgctttat ccaacaccta ttgccaagct aattgatagt tattctaagt taccgggtat
    24721 cgggattaag acggctacgc gtctggcctt ttataccatt ggaatgtctg atgacgacgt
    24781 caatgaattt gccaaaaatc tcctttctgc taagagagag ctgacctact gttctatttg
    24841 tggtcgattg actgatgatg atccttgttc tatctgtacc gatccgactc gtgaccagac
    24901 aacgattttg gtgctagagg atagtcgtga tgtggcggcc atggaaaata tccaagaata
    24961 ccatggactt tatcatgtcc tgcatggtct catttctccc atgaatggca ttagtccaga
    25021 tgatatcaat ctcaagagcc tcatgacccg tcttatggat agtgaggttt cagaagtgat
    25081 tgtagccaca aatgccacag cagatgggga agcgacttcg atgtaccttt cccgcctgct
    25141 caagccagct gggataaagg ttactcgtct ggcgcgaggc ctcgctgtgg gagcggatat
    25201 cgagtatgcg gacgaagtga ccctcttacg agctattgaa aatcggacag agttgtaagt
    25261 ctagacaaat taacaaactc aaattcattt atacaaaaat cacggagacc gacagttgtt
    25321 ttatcggtct ctttttagat ttcttgaaca ccagtatcag gttcaagttt caaaaaagaa
    25381 gcaaatatga tatactaaag aacgagtatt ctattagaat tggaacaaat aatatgaaac
    25441 aaacgattat tcttctatac ggtggacgta gtgcagagcg cgaagtctct gttctttcag
    25501 cggaaagtgt gatgcgtgcg gtcaattacg atcgtttcac agtcaagact ttctttatca
    25561 gccagtcagg tgactttatc aaaacgcagg aatttagtca gactccaggt caagaggacc
    25621 gtctcatgac caatgcgacc attgattggg ataagcaggt tgcaccaagt gccatctacg
    25681 aagaaggcgc agtggtcttt ccagttcttc atggtccgat gggagaagat ggctctgttc
    25741 aaggattctt ggaagttttg aaaatgcctt atgtcggctg caacatcttg tcatccagtc
    25801 ttgccatgga taaaatcacg actaaacgtg ttttggaatc tgctggtatt gcccaagttc
    25861 cttatgtggc catcgttgaa ggtgatgatg tgactgctaa aattgctgaa gtggaagaaa
    25921 aattgactta tccagtcttc accaaaccat caaacatggg ttcaagtgtc ggtatttcta
    25981 agtctgaaaa tcaagaagaa ctccgtcaag ctttgaaact tgccttccaa tatgacagcc
    26041 gtgtcttggt tgagcaaggg gtgaatgccc gtgaaatcga ggttggtctc ttgggcaact
    26101 acgatgtcaa aagcacgctg ccaggagaag ttgtcaaaga tgttgccttt tatgactatg
    26161 acgccaagta tattgataac aagattacta tggatattcc agccaagatc agtgatgatg
    26221 tagtagctgt catgcgtcga aatgcagaaa cggccttccg tgcgattggt ggactaggtc
    26281 tctctcgttg cgatttcttc tatacagaca agggcgagat tttcctaaac gagctaaata
    26341 ccatgccagg tttcacccag tggtctatgt acccactgct ttgggataat atgggaatca
    26401 gctacccaga cctaatcgag catttggttg accttgccaa ggaaagtttt gacaagcgcg
    26461 aagcgcattt gctataaaaa tgaaagagag ggtagaagtc agaaccatca ctgccaggtg
    26521 atcagagttc tcggacttca acccttttta aaggagtaga aatgaaactt acaattcacg
    26581 aagttgccca agctgttgga gctaaaaata atgtcagcat ctttgaggac atccagttag
    26641 aaaaggttga gtttgacagt cgtttgattg ggactggaga tttatttgtg ccactcaagg
    26701 gagcgcgtga tggtcatgac tttatcgaaa cagcatttga aaatggtgca gcagtaacct
    26761 tgtctgagaa agaggtcgca aatcatcctt atattttagt agacgatgtg ttgactgcct
    26821 ttcaacaact agctgcctac tatcttgaga aaactgctgt tgatgtcttt gcagttacag
    26881 gttcaaatgg taagacgacg accaaggata tgttggcgca cttgctttca acaacctata
    26941 aaacctacaa aacacaagga aactacaata acgagattgg ccttccttac acagttctac
    27001 acatgcctga ggggacagaa aagttggttt tggagatggg gcaggatcac ttgggagata
    27061 ttcatctctt gtcagaattg gcccatccaa aaacagccat tgtgaccttg gttggagagg
    27121 ctcatttggc ctttttcaaa gatcgttcgg agatcgctaa aggaaaaatg caaattgcag
    27181 atggtatggc accaggttct ttgcttttgg caccagctga ctcgattgta gaggcctatt
    27241 tgcctacaga taaaaaagtg gtccgttttg ggcaaggagc agagttggaa atcacagact
    27301 tggttgagcg caaggatagt ctgaccttta aggctaattt cttggagcaa gcccttgact
    27361 tgccagtgac tggtaagtac aatgccacta atgctatgat tgcgtcttat gttgccctac
    27421 aagaaggagt atcagaggag caaattcgtc aggccttaca aaatcttgaa ttgacgcgta
    27481 accgtaccga gtggaagaaa gcagccaatg gagcagatat tttgtctgat gtatacaatg
    27541 ccaatccgac tgctatgaag ctgattttgg agactttctc tgccattcca gctaatgaag
    27601 ggggcaagaa aatcgctgtc ttggcagaca tgaaggaact cggtgaccag tctgttcagc
    27661 tccataacca gatgattttg agtctctcgc cagatgtgtt ggataccgtt attttctacg
    27721 gagaagacat tgccgaattg gcccaactgg ctagtcaaat gttcccaatt ggccacgttt
    27781 actacttcaa gaaaacagat gaccaagacc aatttgaaga cctagtcaag caggtcaagg
    27841 aaagccttgg ggctcatgat caaatcctgc tcaaaggctc taactctatg aacctagcta
    27901 agctggtaga aagtttagaa catgaagaca agtgattttg tcaagtattt gcaaagaatg
    27961 attgccatta cagatactgg attaaccttt acaaaggatc cgtttgaccg tgagcgctac
    28021 gaagacttgc gaggcctttt atctgaaatg ttgaatcaag catcagacct tgatgccgaa
    28081 gaagtggcag aagtcttgaa tccaacttct gcttatgcga ctccgttaat ggacgtccgt
    28141 gcttggattg ttgaggagga aaagatttgt ctggttaggg gacaaggaga gaatgattgg
    28201 gctttgccag gtggctttgg tgaggttggc tattctccaa ccgaaaatat tctcaaggaa
    28261 attgaagaag aaaccggttt taaagccaaa gttgaaagac tgctagctgt ttttgatacc
    28321 aatcgtttcc aactacagag caaacaatat gcaaagtttg tttttgaatg taagtttctc
    28381 gatggacaat ttcaagaaaa tcaagaaatc gctgaccttc aattttttgc cattgaccag
    28441 ctgccgaact tgtctgaaaa acgcattacc aaggagcaaa tcaagattct ttggcaggtt
    28501 tatcaaggtc agagggagca atatcttgac taagaagatg attatcgtat ttctaaatcc
    28561 atttttgaca actagcatgg tataataata tgcaggaaaa ttttgaatca tgaggaagac
    28621 tagatgaatt tatgggatat tttctttacg actcaggcaa ccgagccgcc caaatttgac
    28681 cttttttggt atgttagcct atttacgctc ttagccttaa ccttttatac agcccatcgc
    28741 tatcgtgaaa agaaggttta ccaacgattt ttccaaatct tgcagactgt tcagttaatc
    28801 cttctttatg gttggtactg ggtcaatcat atgccgctgt cagaaagcct acccttttac
    28861 cattgccgta tggctatgtt tgtggtactc ttgcttcctg gtcagtccaa atataaacaa
    28921 tactttgcat tattgggaac atttgggaca ttagcagcct ttgtttatcc agtgccagat
    28981 gcttaccctt gtccacatat caccattcta tcctttatct ttggtcattt agcactcttg
    29041 gggaactctc tagtttatct attgagacag tataatgcgc gattgctgga tgtgaaggga
    29101 atttttctca tgacctttgc cctaaatgcc ttgatttttg tggtcaattt ggtgacaggt
    29161 ggcgattacg gatttttgac aaaaccgcca ttggttgggg atcacggtct agtagctaat
    29221 tatttacttg tttcaattgt gctggtagct actatcagtt tgactaagaa aatcttagaa
    29281 ttctttttag ctcaagaagc agaaaaaatg attgcaaagg aagcttaaca cagagctttc
    29341 ttttttgctc ttagagagtt tttacaagca gcttataaaa taagaatttc tgaatagaca
    29401 aactcaaaaa atggctggga aatttaggaa aaaagcaagc acgattaaat tttttgtgtt
    29461 ataatatttt gtgaatagct atgcctatgt ttagctatgg aataatacga agtgcgaaac
    29521 ttggaagata gagaggaagc gatgtaatgg ctagagaagg cttttttaca ggtctagata
    29581 ttggaacaag ctctgtcaag gtgcttgtgg ccgagcagag aaatggtgaa ttaaatgtaa
    29641 ttggcgtgag taatgccaaa agtaaaggtg taaaggatgg aattattgtt gatattgatg
    29701 cagcagcaac tgctatcaag tcagccattt cccaagcgga agaaaaggca ggcatttcga
    29761 ttaaatcagt aaatgtcggc ttgcctggta atcttttgca ggtagaacca actcagggga
    29821 tgattccagt aacatctgat actaaggaaa ttacggatca agatgttgaa aatgttgtca
    29881 aatcagcttt gacaaagagt atgacacctg accgtgaagt cattaccttt attcctgaag
    29941 aatttattgt ggatggtttc caagggattc gtgacccacg tggcatgatg ggggttcgcc
    30001 ttgaaatgcg tggtttgctt tatacaggac ctcgtactat cttgcacaat ttgcgtaaga
    30061 cggttgagcg tgcaggtgtt caggttgaaa atgttatcat ttcaccacta gcaatggttc
    30121 agtctgtttt gaacgaaggg gaacgtgaat ttggtgctac agtgattgat atgggggcag
    30181 gtcaaacgac tgtcgctaca atccgtaatc aagaactcca gttcacacat attctccaag
    30241 aaggtggaga ttatgtaact aaagatatct ccaaggtttt gaaaacctct cgcaaattag
    30301 cggaaggctt gaaactgaat tacggggaag cctatccgcc tcttgcaagc aaagaaacct
    30361 tccaagtaga ggttattgga gaagtagaag cagtcgaagt gacggaagcc tacttgtcag
    30421 aaattatttc tgcacgaatc aagcacatcc ttgaacaaat caagcaagaa ttagatagaa
    30481 ggcgtctatt ggacctccct ggtggtattg tcttaatcgg tgggaatgcc attttaccag
    30541 gtatggttga gcttgctcag gaagtctttg gcgtccgtgt caagctttat gttccaaatc
    30601 aagttggtat ccgtaatcca gcctttgcgc atgtgattag tttatcagaa tttgcgggtc
    30661 aattaacaga agttaatctt ttggctcagg gagcgataaa aggtgagaat gacttaagtc
    30721 atcagccaat tagttttggt gggatgctgc aaaaaacagc tcagtttgta caatcaacgc
    30781 ctgttcaacc agctcctgct ccagaagtag agtcggtggc gcctacagaa ccaatggcgg
    30841 atttccaaca agcttcacaa aataaaccga aattagcaga tcgtttccgt ggcttgatcg
    30901 gaagcatgtt tgacgaataa agaggaaaaa taaattatga cattttcatt tgatacagct
    30961 gctgctcaag gggcagtgat taaagtaatt ggtgtcggtg gaggtggtgg caatgccatc
    31021 aaccgtatgg tcgacgaagg tgttacaggc gtagaattta tcgcagcaaa cacagatgta
    31081 caagcattga gtagtacaaa agctgagact gttattcagt tggggcctaa attgactcgt
    31141 ggtttgggtg caggaggtca acctgaggtt ggtcgtaaag ccgctgaaga aagcgaagaa
    31201 acactgacgg aagctattag tggtgccgat atggtcttca tcactgctgg tatgggagga
    31261 ggctctggaa ctggagctgc tcctgttatt gctcgtatcg ccaaagattt aggtgcgctt
    31321 acagttggtg ttgtaacacg tccctttggt tttgaaggaa gtaagcgtgg acaatttgct
    31381 gtagaaggaa tcaatcaact tcgtgagcat gtagacactc tattgattat ctcaaacaac
    31441 aatttgcttg aaattgttga taagaaaaca ccgcttttgg aggctcttag cgaagcggat
    31501 aacgttcttc gtcaaggtgt tcaagggatt accgatttga ttaccaatcc aggattgatt
    31561 aaccttgact ttgccgatgt gaaaacggta atggcaaaca aagggaatgc tcttatgggt
    31621 attggtatcg gtagtggaga agaacgtgtg gtagaagcgg cacgtaaggc aatctattca
    31681 ccacttcttg aaacaactat tgacggtgct gaggatgtta tcgtcaacgt tactggtggt
    31741 cttgacttaa ccttgattga ggcagaagag gcttcacaaa ttgtgaacca ggcagcaggt
    31801 caaggagtga acatctggct cggtacttca attgatgaaa gtatgcgtga tgaaattcgt
    31861 gtaacagttg ttgcaacggg tgttcgtcaa gaccgcgtag aaaaggttgt gtctccacaa
    31921 gctagaactg ctactaacta ccgtgagaca gtgaaaccag ctcattcaca tggctttgat
    31981 cgtcattttg atatggcaga aacagttgaa ttgccaaaac aaaatccacg tcgtttggaa
    32041 ccaactcagg catctgcttt tggtgattgg gatcttcgcc gtgaatcgat tgttcgtaca
    32101 acagattcag tcgtttctcc agtcgagcgc tttgaagccc caatttcaca agatgaagat
    32161 gaattggata cacctccatt tttcaaaaat cgttaagtaa atgaatgtaa aagaaaatac
    32221 agaacttgtt tttcgagaag ttgcagaggc tagtctgagt gctcatcgag agagtggttc
    32281 ggtctctgtc attgcagtta ccaagtatgt agatgtaccg acagcggaag ccttgcttcc
    32341 gctaggtgtt catcatatcg gtgaaaatcg tgtagataag tttctggaaa aatatgaagc
    32401 tttaaaagat cgagatgtga cttggcattt gattggtacc ttgcaaagac gtaaggtgaa
    32461 agatgtcatt caatacgttg attatttcca tgcattggac tcagtaaagc tagcagggga
    32521 aattcaaaaa agaagtgacc gagtcatcaa gtgtttcctt caagtaaata tttctaaaga
    32581 agaaagcaaa cacggttttt cgagagagga actgctggaa atcttgccag agttagccag
    32641 actagataag attgaatatg ttggtttaat gacgatggca ccttttgagg ctagcagtga
    32701 gcagttgaaa gagattttca aggcggccca agatttacaa agagaaattc aagagaaaca
    32761 aattccaaat atgcctatga ccgagttaag tatgggaatg agtcgtgatt ataaagaagc
    32821 gattcaattc ggttccactt ttgttcgtat aggtacatca ttttttaagt aggagagaac
    32881 catgtcttta aaagatagat tcgatagatt tatagattat tttacggagg atgaggattc
    32941 aagtctccct tatgaaaaaa gagatgagcc tgtgtttact ccagtaaatt cttcacagga
    33001 accggctctc ccaatgaatc aaccttcaca gtcggctggt acaaaagaga acaatatcac
    33061 cagacttcat gcaagacaac aggaattggc aaatcagagt cagcgtgcaa cggataaggt
    33121 cattatagat gttcgttatc ctagaaaata tgaggatgca acagaaattg ttgatttatt
    33181 ggcaggaaac gaaagtatct tgattgattt tcagtatatg acagaggtgc aggctcgtcg
    33241 ttgtttggac tatttggatg gagcttgtca tgttttagct ggaaatttga aaaaggtagc
    33301 ttctaccatg tatttgttga caccagtgaa tgttattgta aatgttgaag atatccgttt
    33361 accagatgaa gatcaacagg gtgagttcgg ttttgatatg aagcgaaata gagtacgata
    33421 atgatttttt taattcgtat gatttataat gcagtggata tttactccct gattttggta
    33481 gccttcgctg tcatgtcttg gtttccaggt gcctacgaat ccagtttagg tcgttggatt
    33541 gtagcgttgg tgaaaccagt gcttgctccc ttgcaacgcc tgcctttaca gatagcgggt
    33601 cttgatttat ctgtttgggt tgcgattgtt ttggttcgat ttttaggaga aaacctagtg
    33661 cgttttctgg cgatgatagg atgaataaag ggatttatca gcatttctcc atagaagatc
    33721 gtccatttct tgacaaggga atggaatgga taaagaaggt agaagatagc tatgctcctt
    33781 ttttaactcc ttttatcaat cctcatcagg agaagctatt aaagattttg gccaaaacct
    33841 atggtcttgc ttgtagcagt agtggggaat tcgtctcgag tgagtatgtt cgagttttat
    33901 tatacccaga ttatttccaa ccagagtttt cagattttga aatatctctc caggaaattg
    33961 tgtattccaa taaatttgaa tatttaacgc atgctaagat tttagggaca gtcatcaatc
    34021 aattagggat tgaacggaaa ctttttggag atatcctagt agatgaagaa cgggcgcaga
    34081 ttatgattaa tcagcagttt cttcttctct tttaagatgg actaaagaaa attggtcgta
    34141 tacctgtttc gctggaggaa cgtcctttca ccgagaaaat agataagcta gaacagtatc
    34201 gagaactgga tttatctgtg tctagttttc gattagatgt tcttttatca aatgttttga
    34261 aactatctag gaatcaagca aaccagttga ttgaaaagaa acttgtccaa gtaaattatc
    34321 atgtggtaga caaatcagat tacactgttc aagttggaga cttgattagt gtgagaaaat
    34381 ttggtcgctt gagattactt caagataagg gacaaacgaa aaaagagaag aaaaaaataa
    34441 ccgtccagtt attattaagt aagtgaggaa tagaatgcca attacatcat tagaaataaa
    34501 ggacaagact tttggaactc gattcagagg ttttgatcca gaagaagtcg atgaattttt
    34561 agatattgtg gttcgtgatt acgaagatct tgtgcgtgcg aatcatgata aaaatttgcg
    34621 tattaagagt ttagaagagc gtttgtctta ctttgatgaa ataaaagatt cattgagcca
    34681 gtctgtattg attgcccagg atacagctga gcgagtgaaa caggcggcgc atgaacgttc
    34741 aaacaatatc attcatcaag cagagcaaga tgcgcaacgc ttgttggaag aagctaaata
    34801 taaggcaaac gagattcttc gtcaagcaac tgataatgct aagaaagtcg ctgttgaaac
    34861 agaagaattg aagaacaaga gccgtgtctt ccaccaacgt ctcaaatcta caattgagag
    34921 tcagttggct attgttgaat cttcagattg ggaagatatt ctccgtccaa cagctactta
    34981 tcttcaaacc agtgatgaag cctttaaaga agtggttagc gaagtacttg gagaaccgat
    35041 tccagttcca attgaagaag aaccaattga tatgacacgt cagttctctc aagcagaaat
    35101 ggaagaatta caagctcgta ttgaggtagc cgataaagaa ttgtctgaat ttgaagctca
    35161 gattaaacag gaagtggaag ctccaactcc tgtagtgagt cctcaagttg aagaagagcc
    35221 tctgctcatc cagttggccc aatgtatgaa gaaccagaag tagctccagt gcatccgaca
    35281 ggtccaacac cagctacaga aactgttgat tcagcaccgg gatttgaagc accgcaagaa
    35341 tctgttacaa ttttataaga aatattctga gaacaatatc ttatccttat attcccagcg
    35401 agcaggagat ggtgtgagtc ctgcattccc tatcgataag attatcctct caaaaactca
    35461 agtctgaatc tagtaagatt tgacgttccc cacgttacgg gataagaggg agaaagacta
    35521 aaactttttc cgaataaagg tggtaccacg attttcgtcc tttttggaag tcgtggtttt
    35581 taatttgtta ttatttataa aggagatacc atgaaactca aagacaccct taatcttggg
    35641 aaaactgaat tcccaatgcg tgcaggcctt cctaccaaag agccagtttg gcaaaaggaa
    35701 tgggaagatg caaaacttta tcaacgtcgt caagaattga accaaggaaa acctcatttc
    35761 accttgcatg atggccctcc atacgctaac ggaaatatcc acgttggaca tgctatgaac
    35821 aagatttcaa aagatattat tgttcgttct aagtctatgt caggatttta cgcaccattt
    35881 attcctggtt gggatactca tggtctgcca atcgagcaag tattgtcaaa acaaggtgtc
    35941 aaacgtaaag aaatggactt ggttgagtac ttgaaacttt gccgtgagta cgctctttct
    36001 caagtagata aacaacgtga agattttaaa cgtttgggtg tttctggtga ctgggaaaat
    36061 ccatatgtga ccttgactcc tgactatgaa gcagctcaaa ttcgtgtatt tggtgagatg
    36121 gctaataagg gttatatcta ccgtggtgcc aagccagttt actggtcatg gtcatctgag
    36181 tcagcccttg ctgaagcaga gattgaatac catgacttgg tttcaacttc cctttactat
    36241 gccaacaagg taaaagatgg caaaggagtt ctagatacag atacttatat cgttgtctgg
    36301 acaacgactc catttaccat cacagcttct cgtggtttga cggttggtgc agatattgat
    36361 tacgttttgg ttcaacctgc tggtgaagct cgtaagtttg tcgttgctgc tgaattattg
    36421 actagcttgt ctgagaaatt tggctgggct gatgttcaag ttttggaaac ttaccgtggc
    36481 caagaactca accacatcgt aacagaacac ccatgggata cagctgtaga agagttggta
    36541 attcttggtg accacgttac gactgactct ggtacaggta ttgtccatac agcccctggt
    36601 tttggtgagg acgattacaa tgttggtatt gctaataatc ttgaagtcgc agtgactgtt
    36661 gatgaacgtg gtatcatgat gaagaatgct ggtcctgaat ttgaaggtca attctatgaa
    36721 aaggtagttc caactgttat tgaaaaactt ggtaacctcc ttcttgccca agaagaaatc
    36781 tctcactcat atccatttga ctggcgtact aagaaaccaa tcatttggcg tgcagttcca
    36841 caatggtttg cctcagtttc taaattccgt caagaaatct tggacgaaat tgaaaaagtg
    36901 aaattccact cagaatgggg taaagtccgt ctttacaata tgatccgtga ccgtggtgac
    36961 tgggttatct ctcgtcaacg tgcttggggt gttccacttc ctatcttcta cgctgaagat
    37021 ggtacagcta tcatggtagc tgaaactatt gaacacgtag ctcaactttt tgaagaacat
    37081 ggttcaagca tttggtggga acgtgatgcc aaagacctct tgccagaagg atttactcat
    37141 ccaggttcac caaacggcga gttcaaaaaa gaaactgata tcatggacgt ttggtttgac
    37201 tcaggttcat catggaatgg agtggtggta aaccgtcctg aattgactta cccagccgac
    37261 ctttacctag aaggttctga ccaataccgt ggttggttta actcatcact tatcacatct
    37321 gttgccaacc atggcgtagc accttacaaa caaatcttgt cacaaggttt tgcccttgat
    37381 ggtaaaggtg agaagatgtc taaatctctt ggaaatacca ttgctccaag cgatgttgaa
    37441 aaacaattcg gtgctgaaat cttgcgtctc tgggtaacaa gtgttgactc aagcaatgac
    37501 gtgcgtatct ctatggatat cttgagccaa gtttctgaaa cttaccgtaa gattcgtaac
    37561 actcttcgtt tcttgattgc caatacatct gactttaacc cagctcaaga tacagtcgct
    37621 tacgatgagc ttcgttcagt tgataagtac atgacgattc gctttaacca gcttgtcaag
    37681 accattcgtg atgcctatgc agactttgaa ttcttgacga tctacaaggc cttggtgaac
    37741 tttatcaacg ttgacttgtc agccttctac cttgattttg ccaaagatgt tgtttacatt
    37801 gaaggtgcca aatcactgga acgccgtcaa atgcagactg tcttctatga cattcttgtc
    37861 aaaatcacca aactcttgac accaatcctt cctcacactg cggaagaaat ttggtcatat
    37921 cttgagtttg aaacagaaga cttcgtccaa ttgtcagaat taccagaggc tcaaactttt
    37981 gctaatcaag aagaaatctt ggatacatgg gcagccttca tggacttccg tggacaagct
    38041 caaaaagcct tggaagaagc tcgtaatgca aaagtaatcg gtaaatcact tgaagcacac
    38101 ttgacagttt atccaaacga agttgtgaaa actctactcg aagcagtaaa cagcaatgtg
    38161 gctcaacttc tgatcgtgtc agacttgacc atcgcagaag gaccagctcc agaagctgcc
    38221 cttagcttcg aagatgtagc cttcacagtt gaacgcgctg caggtgaagt atgtgaccgt
    38281 tgccgtcgta ttgacccaac aacagcagaa cgtagctacc aggcagttat ctgtgaccac
    38341 tgtgcaagca tcgtagaaga aaactttgcg gatgcagtcg cagaaggatt tgaagagaaa
    38401 taagattgaa aagtctaggc aaaattcaat ttgagaagaa acgacaacta attttatagt
    38461 ctattaaacg cattgtatca cgtttttgaa cacctgatat gatgcgtttt tatttatttt
    38521 gaaaatttgt gaggtgtgac tttttatact caacaagaat caaagagaaa cttagcaagc
    38581 taacagtagt aagataaaat aggaatttga tattagggat aagattggta aatagtgtaa
    38641 tatttttaca acaataaatt tatatagtta tttct
//
LOCUS       DAVKJU010000014        37424 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVKJU010000014 DAVKJU010000000
VERSION     DAVKJU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.014"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(48..1715)
                     /locus_tag="VW191_000608"
     CDS             complement(48..1715)
                     /locus_tag="VW191_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEU9623422.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(1718..2083)
                     /locus_tag="VW191_000609"
     CDS             complement(1718..2083)
                     /locus_tag="VW191_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9623423.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(2240..2428)
                     /locus_tag="VW191_000610"
     CDS             complement(2240..2428)
                     /locus_tag="VW191_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU9623424.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(2544..2720)
                     /locus_tag="VW191_000611"
     CDS             complement(2544..2720)
                     /locus_tag="VW191_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9623425.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENITQDLI"
     gene            complement(2831..3028)
                     /locus_tag="VW191_000612"
     CDS             complement(2831..3028)
                     /locus_tag="VW191_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEU9623426.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(3456..5000)
                     /locus_tag="VW191_000613"
     CDS             complement(3456..5000)
                     /locus_tag="VW191_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEU9623427.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            5213..5515
                     /gene="gatC"
                     /locus_tag="VW191_000614"
     CDS             5213..5515
                     /gene="gatC"
                     /locus_tag="VW191_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU9623428.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVVEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            5515..6981
                     /gene="gatA"
                     /locus_tag="VW191_000615"
     CDS             5515..6981
                     /gene="gatA"
                     /locus_tag="VW191_000615"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU9623429.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKHSEETIYQVAAAFEATTGYHKQQPVIFGGDN"
     gene            6981..8423
                     /gene="gatB"
                     /locus_tag="VW191_000616"
     CDS             6981..8423
                     /gene="gatB"
                     /locus_tag="VW191_000616"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU9623430.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAIADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            8552..9112
                     /gene="efp"
                     /locus_tag="VW191_000617"
     CDS             8552..9112
                     /gene="efp"
                     /locus_tag="VW191_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU9623431.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            9134..9523
                     /locus_tag="VW191_000618"
     CDS             9134..9523
                     /locus_tag="VW191_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9623432.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGHGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            9516..9938
                     /gene="nusB"
                     /locus_tag="VW191_000619"
     CDS             9516..9938
                     /gene="nusB"
                     /locus_tag="VW191_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU9623433.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(9987..10214)
                     /locus_tag="VW191_000620"
     CDS             complement(9987..10214)
                     /locus_tag="VW191_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9623434.1"
                     /translation="MLSGLIFGLSHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRL
                     TILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(10705..10890)
                     /gene="briC"
                     /locus_tag="VW191_000621"
     CDS             complement(10705..10890)
                     /gene="briC"
                     /locus_tag="VW191_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEU9623435.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(11049..11816)
                     /locus_tag="VW191_000622"
     CDS             complement(11049..11816)
                     /locus_tag="VW191_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEU9623436.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(11813..12679)
                     /locus_tag="VW191_000623"
     CDS             complement(11813..12679)
                     /locus_tag="VW191_000623"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEU9623437.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPSCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(12716..14083)
                     /gene="accC"
                     /locus_tag="VW191_000624"
     CDS             complement(12716..14083)
                     /gene="accC"
                     /locus_tag="VW191_000624"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU9623438.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(14095..14517)
                     /gene="fabZ"
                     /locus_tag="VW191_000625"
     CDS             complement(14095..14517)
                     /gene="fabZ"
                     /locus_tag="VW191_000625"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEU9623439.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(14514..14999)
                     /locus_tag="VW191_000626"
     CDS             complement(14514..14999)
                     /locus_tag="VW191_000626"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU9623440.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(15002..16237)
                     /gene="fabF"
                     /locus_tag="VW191_000627"
     CDS             complement(15002..16237)
                     /gene="fabF"
                     /locus_tag="VW191_000627"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU9623441.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(16259..16990)
                     /gene="fabG"
                     /locus_tag="VW191_000628"
     CDS             complement(16259..16990)
                     /gene="fabG"
                     /locus_tag="VW191_000628"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU9623442.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(17024..17944)
                     /gene="fabD"
                     /locus_tag="VW191_000629"
     CDS             complement(17024..17944)
                     /gene="fabD"
                     /locus_tag="VW191_000629"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU9623443.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEATPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(17937..18911)
                     /gene="fabK"
                     /locus_tag="VW191_000630"
     CDS             complement(17937..18911)
                     /gene="fabK"
                     /locus_tag="VW191_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEU9623444.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(19030..19254)
                     /locus_tag="VW191_000631"
     CDS             complement(19030..19254)
                     /locus_tag="VW191_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9623445.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(19314..20288)
                     /locus_tag="VW191_000632"
     CDS             complement(19314..20288)
                     /locus_tag="VW191_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEU9623446.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(20288..20722)
                     /locus_tag="VW191_000633"
     CDS             complement(20288..20722)
                     /locus_tag="VW191_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9623447.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(20817..21602)
                     /locus_tag="VW191_000634"
     CDS             complement(20817..21602)
                     /locus_tag="VW191_000634"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU9623448.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            21927..23291
                     /locus_tag="VW191_000635"
     CDS             21927..23291
                     /locus_tag="VW191_000635"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU9623449.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRELTPIKEEEILRQLVQKTKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGK
                     SKED"
     gene            23294..23665
                     /locus_tag="VW191_000636"
     CDS             23294..23665
                     /locus_tag="VW191_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEU9623450.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            23878..25152
                     /gene="serS"
                     /locus_tag="VW191_000637"
     CDS             23878..25152
                     /gene="serS"
                     /locus_tag="VW191_000637"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU9623451.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(25178..26077)
                     /locus_tag="VW191_000638"
     CDS             complement(25178..26077)
                     /locus_tag="VW191_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEU9623452.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(26186..26734)
                     /locus_tag="VW191_000639"
     CDS             complement(26186..26734)
                     /locus_tag="VW191_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU9623453.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(26952..28274)
                     /locus_tag="VW191_000640"
     CDS             complement(26952..28274)
                     /locus_tag="VW191_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEU9623454.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(28365..28453)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(28617..30953)
                     /locus_tag="VW191_000641"
     CDS             complement(28617..30953)
                     /locus_tag="VW191_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEU9623455.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKEIELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(31057..31605)
                     /locus_tag="VW191_000642"
     CDS             complement(31057..31605)
                     /locus_tag="VW191_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU9623456.1"
                     /translation="MISLLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(31602..31904)
                     /gene="zapA"
                     /locus_tag="VW191_000643"
     CDS             complement(31602..31904)
                     /gene="zapA"
                     /locus_tag="VW191_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU9623457.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            31991..32872
                     /locus_tag="VW191_000644"
     CDS             31991..32872
                     /locus_tag="VW191_000644"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEU9623458.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            32876..33490
                     /gene="lepB"
                     /locus_tag="VW191_000645"
     CDS             32876..33490
                     /gene="lepB"
                     /locus_tag="VW191_000645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU9623459.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            33618..35984
                     /locus_tag="VW191_000646"
     CDS             33618..35984
                     /locus_tag="VW191_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEU9623460.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPVDFTQKKADRSYFEITSGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(36032..37315)
                     /locus_tag="VW191_000647"
     CDS             complement(36032..37315)
                     /locus_tag="VW191_000647"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU9623461.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        10848 a         8623 c         7252 g        10701 t
ORIGIN      
        1 tttactaaaa aagaaaaaag aactgatttc tcagtccttc attaatctta ttccacacta
       61 aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac gtcttcgaat
      121 tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc ttcaccgaca
      181 tagaaggtta cgatttcact gtcttcatcc aacatatgtt tcaaggtttc agtcaatgtt
      241 tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag attatcgttt
      301 tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac gcttccgcta
      361 acgacatcgc taagagcagc tgtcatacgc tcttgatttt cttcaatgga cttgcttgga
      421 tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac cactactgct
      481 ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt gttgtttggc
      541 aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc ttctgttgaa
      601 gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca gaagatatct
      661 gctagacctt taccagctac cacagcaatc aaagcatact ctttttcttc agccgacttg
      721 ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat attgtcaact
      781 tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc tggatcttct
      841 gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga atccccaagc
      901 tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt tggaccttgc
      961 ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt cgctacgtga
     1021 ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc aggagtcgct
     1081 acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc ttcgtagatg
     1141 aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac tggaagcatg
     1201 tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat gacttcaaca
     1261 gtatcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg agaaactgtc
     1321 aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac acctgattgg
     1381 aaggccagag ccaagtcttg acctgttaac tcgtctttat ccttgatagc ttgggaaaat
     1441 ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat caaaagccct
     1501 ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc aacttcttta
     1561 gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg aactggaaag
     1621 acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc agcctgcacc
     1681 atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca actttgatat
     1741 tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc aagctaaagg
     1801 caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag cttaacacgg
     1861 tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca cccttagaat
     1921 aattttcttt acctagcagg gcttggaaat tatctttgag ggcattttta ctagccatac
     1981 cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc acttcatctg
     2041 ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt acctcaacta
     2101 gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga tgtatcagcg
     2161 gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa agaccctaag
     2221 gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa gcacgagctg
     2281 aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga aggtttggtt
     2341 ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat acagtcttac
     2401 gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc taatatagcg
     2461 gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt tgcaagggaa
     2521 ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgatattttc tgctctccac
     2581 atgccattgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa atcatctcta
     2641 actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat cgattttctt
     2701 acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt tggaataaaa
     2761 tacctaaaaa agccgtatat gaaagtccct caaaggggag acgataacct ttcagaatac
     2821 tactgtcaat ctaaacattc acaatctcac tattcaaagt ctctagtctt ttttccatca
     2881 attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga ttataatgat
     2941 gaacacaaag agattttatt gttgtttcaa ctttatccat ataagcatta tctgcaccta
     3001 agacaatcgc ttttttctct tctttcactt tttatctcat ttctttttat tcccatcata
     3061 tgtttctaca taaccattat tttcgcctat tcgttcgtaa aaccatacca gtggagattt
     3121 tagatgaagt cccattacgg tttacaattt ttacattacg acacggagtt ttacaaatcg
     3181 atttcatttg ccaaacgtag ttagtaaggc agttagctag gctacctact ttctgctacg
     3241 acggaaaaat ccacaaatca ataccattcg tggatttttc ctagtgaagc gtttaggtaa
     3301 gccgccatag cggttaccga ttattagatt acgacacgga gtttggcaaa tcgattctat
     3361 ttgccaaacg tagttagtaa ggcagttagc tagttcacca aatagcgact agcgtccaac
     3421 aatttggaac tttagttcca attgttggta ctgagtcaca tcttctcctc taactctacg
     3481 tctggatact tgtccgcaaa ccagcggagg gcaaagtcat tttcaaagag aaagactggt
     3541 tggtcaaaac ggtctttggc taagatattg cgacttgacg acatccgttc atccaagtcc
     3601 tcaggcttaa tccaacgaac ggtcttttta cccattgggg tcataactac ttccgcattg
     3661 tactcgcctt ccatgcggtg tttaaagact tcaaactgga gttgacctac agcgcctagc
     3721 atgtactcac ctgtttggta attcttataa agctgaacgg ctccttcttg caccaattgc
     3781 tcaatcccct tgtggaagga tttttgcttc ataacattct tagcagaaac tttcatgaaa
     3841 atttcaggag taaaggttgg caggggttca aattcaaact tgttttttcc aaccgtcaag
     3901 gtatccccaa cctgataagt accggtatcg taaaccccga taatatcacc tgccacggca
     3961 ttggtcacat tctcacgact ctccgccata aactgggtaa cattagatag tttggccccc
     4021 ttaccagtac gaggaagatt gacactcatg ccacgctcaa attcgcctga tacgatacga
     4081 acaaaggcaa tacggtcacg gtgacgagga tccatgttgg cttggatttt aaagacaaag
     4141 cctgagaaat ccttgtcata aggatccaca atttcaccgt ctgttttctt gtgaccatgt
     4201 ggttctggag caaacttgag gaaggtttca aggaaggtct gcacaccaaa gtttgtcagg
     4261 gctgaaccga aaaagacagg cgtcaattct ccagccagaa tagcttcctc tgaaaactca
     4321 ttcccagctt catttaaaag ctcaatgtca tccttgactt gctcgtaaaa aggattgcta
     4381 ccaaaaagtt tgtccccatc ttctaggcta gcaaaacgct catccccttt ataaagttct
     4441 aaacgttggt tatagaggtc atacaagccc tcaaaggctt tccccatccc gataggccag
     4501 ttcatagggt agctagcaat gcccaagatt tcttccaatt cttgcaagag atccaaaggc
     4561 tcacgaccgt cacggtccag cttgttcata aaggtaaaaa ctggaatgcc acgatgtttc
     4621 acaacctcaa acaatttctt ggtttgagcc tcgatcccct tggcagagtc cacgaccatg
     4681 accgcagcat ccaccgccat caaggtacga taggtatctt ctgagaagtc ctcgtgccct
     4741 ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa attgcataac agatgaagta
     4801 acagaaatcc cacgttgctt ctcgatatcc atccagtcag atttagcaaa agtccctgtt
     4861 ttcttccctt ttaccgtacc agcctcacga atctcacccc caaagtagag taactgctca
     4921 gtgatggttg ttttccccgc gtccgggtgg gagataatgg caaaggtacg acgtttctta
     4981 atttcttctt gaatattcat aagttctctt tctttgattc tctatttttc ttgtttcaat
     5041 agctgagaat gatttttaca ttggatttta ccattccttt caacactcca ttatatcgga
     5101 ttttagcatt tttttcaatt tctatttctt ttcacttccc cctcccttat ttataggaaa
     5161 atatggtaaa atagaacaga ctaaaaatca tcatttcacg aaaggatgca agatgaaaat
     5221 tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa ttaagattct ctgaagaaga
     5281 aactgctgcc tttgcgacca ccttgtctaa gattgttgac atggttgaat tgctgggcga
     5341 agttgacaca actggtgtcg cacctactac gactatggct gaccgcaaga ctgtactccg
     5401 ccctgatgtg gtcgaagaag gaacagaccg tgatcgcttg tttaaaaacg tacctgaaaa
     5461 agacaactac tatatcaagg tgccagctat cctagacgat ggaggagatg cctaatgact
     5521 tttaacaata aaactattga agagttgcac aatctccttg tctctaaaga aatttctgca
     5581 acagaattga cccaagcaac acttgaaaat atcaagtctc gtgaggaagc cctcaattca
     5641 tttgtcacca tcgctgagga gcaagctctt gttcaagcta aagccattga tgaagctgga
     5701 attgatgctg acaatgtcct ttcaggaatt ccacttgctg ttaaggataa catctctaca
     5761 gacggtattc tcacaactgc tgcctcaaaa atgctctaca actatgagcc aatctttgat
     5821 gcgacagctg ttgccaatgc aaaaaccaag ggcatgattg tcgttggaaa gaccaacatg
     5881 gacgaatttg ctatgggtgg ttcaggtgaa acttcacact acggagcaac taaaaacgct
     5941 tgggaccaca gcaaggttcc tggtgggtca tcaagtggtt ctgccgcagc tgtagcctca
     6001 ggacaagttc gcttgtcact tggttctgat actggtggtt ccatccgcca acctgctgcc
     6061 ttcaacggaa tcgttggtct caaaccaacc tacggaacag tttcacgttt cggtctcatt
     6121 gcctttggta gctcattaga ccagattgga ccttttgctc ctactgttaa ggaaaatgcc
     6181 ctcttgctca acgctattgc cagcgaagat gctaaagact ctacttctgc tcctgtccgc
     6241 atcgccgact ttacttcaaa aatcggccaa gacatcaagg gtatgaaaat cgctttgcct
     6301 aaggaatacc tcggtgaagg aattaaccca gaggttaagg aaaccattct aaatgccgct
     6361 aaacactttg aaaaattggg tgctattgtc gaagaagtca gccttcctca ctctaaatac
     6421 ggagttgccg tatactacat catcgcttca tcagaagctt catcaaactt gcaacgtttt
     6481 gacggtatcc gttacggcta tcgcgcagaa gatgcgacta atcttgatga aatctatgta
     6541 aacagccgta gccaaggttt tggtgaagaa gtgaagcgcc gtatcatgct gggtactttc
     6601 agtctttcat caggttacta cgatgcctac tacaagaagg ctggacaggt ccgtacactt
     6661 atcattcaag atttcgaaaa agtcttcgcg gattacgatt tgattttggg tccaactgct
     6721 ccaagtgttg cctatgactt ggattctctc aaccatgacc cagttgccat gtacttagcc
     6781 gacctattga ccatacctgt aaacttagca ggtctccctg gaatttcgat tcctgctgga
     6841 ttctctcaag gtctacctgt cggtctgcaa ttgattggtc ccaagcactc tgaggaaacc
     6901 atttaccaag ttgctgctgc ctttgaagca acaacaggct accacaaaca acaacccgtg
     6961 atttttggag gtgacaacta atgaactttg aaacagtcat tggacttgaa gtccacgtag
     7021 agctcaacac caattcaaaa atcttctcac ctacttctgc ccacttcgga aatgatcaaa
     7081 atgccaacac taacgtgatt gactggtctt tcccaggagt tctgccagtt ctcaataaag
     7141 gggttgtcga tgccggaatc aaggctgctc ttgccctcaa catggacatc cacaaaaaga
     7201 tgcactttga ccgcaagaac tacttctatc ctgataaccc caaagcctac caaatttctc
     7261 aatttgatga accaatcgga tataatggct ggattgaagt cgaactagaa gacggtacga
     7321 ccaagaaaat cggtatcgaa cgtgcccacc tagaggaaga cgctggtaaa aacacccatg
     7381 gtacagatgg ctactcttat gttgacctca accgccaagg ggttcccttg attgagattg
     7441 tatctgaggc agatatgcgt tctcctgaag aagcctatgc ttatctgaca gccctcaagg
     7501 aagttatcca gtacgctggc atttctgacg ttaagatgga ggaaggttcg atgcgtgtgg
     7561 atgccaacat ctcccttcgt ccttatggtc aagagaaatt cggtaccaag actgaattga
     7621 agaacctcaa ctccttctca aacgttcgta aaggtcttga atacgaagtc caacgccagg
     7681 ctgaaattct tcgctcaggt ggtcaaatcc gccaagaaac acgccgttac gatgaagcga
     7741 ataaagcaac catcctcatg cgtgtcaagg aaggggctgc tgactaccgc tacttcccag
     7801 aaccagacct acccctcttt gaaatttctg acgagtggat tgaggaaatg cggactgagt
     7861 tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc tgaccttggt ttatcagact
     7921 acgatgctag tcagttgact gctaataaag tcacttctga cttctttgaa aaagctgttg
     7981 ccctaggtgg tgatgccaaa caagtctcta actggctcca aggggaagtc gctcagttct
     8041 tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac accagaaaac ttggttgaaa
     8101 tgattgccat catcgaagac ggtactattt catctaagat tgccaagaaa gtctttgtcc
     8161 atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga aaaagcaggt atggttcaaa
     8221 tttcagatcc agctatcttg atcccaatca tccaccaagt ctttgccgat aacgaagctg
     8281 ctattgccga cttcaagtca ggcaaacgta acgccgacaa agcctttaca ggattcctta
     8341 tgaaggcaac caaaggccaa gccaacccac aagttgccct taaactactt gcacaggaat
     8401 tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc ctaaggttgg ttttttcttc
     8461 tctaccaact cccaataact attttggctt tatttccaga gtattttatg gtaaaatgaa
     8521 gagtaataat atttattaaa gaggtaaaaa catgattgaa gcaagtaaat taaaagctgg
     8581 tatgaccttt gaaacagctg acggcaaatt gattcgcgtt ttggaagcta gtcaccacaa
     8641 gccaggtaaa ggaaacacga tcatgcgtat gaaattgcgt gatgtccgta ctggttctac
     8701 atttgacaca agctaccgtc cagaggaaaa atttgaacaa gctattatcg agactgtccc
     8761 agctcaatac ttgtacaaaa tggatgacac agcatacttc atgaatacag aaacttatga
     8821 ccaatacgaa atccctgtag tcaatgttga aaacgaattg ctttacatcc ttgaaaactc
     8881 tgatgtgaaa atccaattct acggaactga agtgatcggt gtcaccgttc ctactactgt
     8941 tgagttgaca gttgctgaaa ctcaaccatc tatcaaaggt gctactgtta caggttctgg
     9001 taaaccagca acgatggaaa ctggacttgt cgtaaacgtt ccagacttca tcgaagcagg
     9061 acaaaaactc gttatcaaca ctgcagaagg aacttacgtt tctcgtgcct aatctctaga
     9121 aagaggtcat tctatgggaa ttgaagaaca acttggcgaa atcgttatcg ccccacgtgt
     9181 acttgaaaaa atcattgcta tcgctactgc aaaggtagag ggtgttcact ctttttcaaa
     9241 cagatcagtg tctgataccc tttcaaaact ttcactcggc catggcattt atcttaaaaa
     9301 cgtggacgaa gaactcacag cagatatcta tctctacctt gagtacggag taaaagttcc
     9361 taaggtagcg gttgctatcc agaaagctgt caaagatgcc gtccgtaata tggctgatgt
     9421 agaactcgct gctatcaata ttcacgttgc aggtatcgtc ccagataaaa caccaaaacc
     9481 agaattgaaa gatctatttg acgaggactt cctcaatgac tagtccacta ttagaatcta
     9541 gacgccaact ccgtaaatgc gcttttcaag ctctcatgag ccttgagttc ggtacggatg
     9601 tcgaaactgc ttgtcgtttc gcctatactc atgatcgtga agatacggat gtacaacttc
     9661 cagccttttt gatagacctc gtttctggtg ttcaagctaa aaaggaagaa ctagataagc
     9721 aaatcactca gcatttaaaa gcaggttgga ccattgaacg cttaacgctc gtggagagaa
     9781 acctccttcg cttgggagtc tttgaaatca cttcatttga cactcctcag ctggttgctg
     9841 ttaatgaagc tatcgagctt gcaaaggact tctccgatca aaaatctgcc cgttttatca
     9901 atggactgct cagccagttt gtaacagaag aacaataaaa agaaaaagtg tttgatatct
     9961 atcaaacact ttttctttgc ctcccactat ctaaaaaaat gataatagat ataattgtaa
    10021 acaaaaatcc agataggttt tgcatgattg agaaagttaa aaaaactatg gcagagaatc
    10081 gttaatctca gattgtcggt agaacgataa acaagggcaa aaaagaaacc aatcagacta
    10141 taatataata aactaattgg atctctgtga gatagtatca aatgactaag cccaaagata
    10201 aggcctgata agatgacatc taaataatac ttggactagg gaaagaaggt gttcataaaa
    10261 taccctctat caagagtctc ctcaaaaaca ggaccgatga ttacaggcag gacaaaagat
    10321 aagatagtcg ataaaaaggt tggttgtcca tttgaaagaa ccacggtaaa atactcatca
    10381 tgaatattcc tatgattaat caaatgagca tagcgtgccc aaaaattacc gagaatctga
    10441 taaaccacat aagttgcaaa taaggagaag acaaatgacc agtcccagct ctttttcttt
    10501 tttaacctcc aaattaatag aaaaggaaac ttcccactaa tcccactgtt aaaataagag
    10561 aatagacatc aactcctaac cctaaaatga tcgtcacata caatccaatt gtttgtggta
    10621 aataggtaga tagtaaaata ataagcaaaa atattccaaa ttgtcttagt ttctttgtgt
    10681 ttctcatcgt acttttttga aagattaccc tgttcggaag ccgtacttcc aagcatctat
    10741 ataagaatta agtgcccctt gcctcatata gggagcaaat tctctataat ataaccatct
    10801 actatatcca tcttcccaaa cagcaagacc acctgaagtt tgctccaagt cctcagttga
    10861 aataactgta aatgtttctg tacctgtcat tgcaagtacc ttcttaaaat agattgttgt
    10921 aggctcacat ttatagtata tttctttttt tgtctatttt atagtctatc tcctcaactg
    10981 gcaatttttc gacctgaatt gcatttttcc ataaaaaatg agacctttct agtctcattt
    11041 agtcattctt agtattttct aaatcgttga tagcgttctt ccagcaactc ttctagcggt
    11101 ttttgtgaaa gtctagccag ctccgtttgg agttcttttt tgacactctt aatcagttct
    11161 ttactagaaa gtcctacttc agaaatcacc ttatccacca catccatttc taacagttca
    11221 tgcgaagtga ttttcatcag ttctgctgct tccatggcgc gagtaccgtc cttccataaa
    11281 atggaagcaa agccttctgg actgagaatg gcatagatag aattttccag catccagaca
    11341 cggtccgcga cagctagagc cagagccccg cctgaaccac cttcaccgat aataatggcg
    11401 ataataggaa ctttcaggtc actcatttcc atgagattgc gagcgatagc ttccccttga
    11461 ccacgttctt ccgctccgac accaggataa gcacctgctg tattaataaa ggtcacaact
    11521 ggacggccaa atttctcagc ctgtttcatc aaccgcagtg cctttcggta gccttctgga
    11581 tgtggttggc caaaattccg tttgaggttg tcttgcaaac tcttgccttt ttggatacca
    11641 accactgtta cagcttggtc tccaagccaa ccaataccac caacaactgc accatcatca
    11701 cgaaaagaac ggtcaccatg taattggata aattcatcaa aaatgcctgt cgcaaagtcc
    11761 aaggttgtca agcgactctg ctcacgcgct tctctgacta tttttgcaat attcatctag
    11821 gactccctcc atgcaatctg actaggctag caatcgtatc tggtaagtct cttcttttga
    11881 caatagcatc cacaaagcca tgttctaata ggaattctgc cttttggaaa tcctcaggca
    11941 agctttcacg aaccgtattt tcaatcacac gacgcccagc aaaaccaacc aagctctgtg
    12001 gttcagccag aatgatatcg ccttccatag cgaaagaagc tgtcacacca ccagtcgttg
    12061 gatctgtcaa aatggtcagg taaaagagac cagcatttga atgacgttta accgccgcag
    12121 agatcttagc catctgcatg agactcatga ttccttcctg catacgggct ccaccagagg
    12181 ctgtgaatag gacaactggc aatttttcga cagtcgcata ctcaaacaaa cgagtgattt
    12241 tttcacctac aaccgtaccc atagaagcca tgataaagtt agaatccata atcccaagag
    12301 ccacagtctg acctttaata agagcagttc ctgtcacaac ggcttcatgc agacctgttt
    12361 tttcacgcat agatgccagt ttcttttggt aaccagggaa atgcaaggga tccttgcttt
    12421 caatccctgt aaacaattct ttgaaggttc ccatatcaat cgtcaaagcc aagcgttctt
    12481 gggcagaaat acgaaaggta tagctacagt gcggacagat acgttcactt cccagatcct
    12541 tctgatagat ggtatgctta cagcttggac actgggaaaa taattcatct ggaacctctg
    12601 gcttagcttg aggtttttcc ctaaccgaac gattgggatt gattcgaata tacttatctt
    12661 ttttactaaa tagagccatt gattcccctt ttcggtttaa actcttaaag tcattttatt
    12721 ctttttcttg atatttaggt aagaaggttt ccatcaagaa ggaagtatca taatccccag
    12781 caatgacatt gcgatctgaa atgaggtcaa gctggaaatc tgcattggtc tgcactcctt
    12841 caatctctaa ttcatagagg gcacgttgca ttttcatcaa ggcgtcaaaa cgattttcgc
    12901 cgtgtactat gattttggca atcatactat cataataagg cggaatggta taacctggat
    12961 aaactgctga atccacgcgc aagccaactc caccacttgg cagatagaga ttagtaatct
    13021 tacctggact tggagcaaag ttaaaggctg ggttttctgc attgatacga cactcgatgg
    13081 catgaccgcg taggacaata tcttcttgct taacagacaa aggctgacct gccgcaatgc
    13141 aaatctgttc cttaacgata tcaacacctg aaacaaactc tgttactgga tgttctacct
    13201 gaacacgagt attcatctcc atgaaataga aattgctact tgcttcatca agaagaaatt
    13261 caatggttcc tgcattctca tagccaacaa actctgccgc tcgaacagca gcagcaccta
    13321 tttcatgacg cagcgttttt ccgattgcaa tcgagggact ttcttccaaa accttttggt
    13381 tattcctttg aagagaacaa tcccgctcac ccaagtgaat cacatgtcca tgctcatcac
    13441 ctaggatttg aacctcaatg tgccgagctg gatagataac ccgttctatg tacatggcac
    13501 cattgccata attggccttg gcctcactag aggcagtttc aaaggcagaa acgaggtcat
    13561 ctggtttttc aaccttacga atccctttac cacctccacc tgctgaagcc ttgagcataa
    13621 caggatagcc aattttttca gcaacaatca aagcttcttc agagttatgc acttctccat
    13681 ctgaacctgg tataacaggc acacctgctt taatcatctg agcacgcgca ttgatcttat
    13741 cccccatcat atccataaca tgaccagatg gaccgataaa cttgatacct acttcttcac
    13801 acatggtcgc aaatttggaa ttttcactga gaaatccaaa accagggtga atagcttctg
    13861 cctcagtcaa gactgcagct gatagaactg cattaatatt gagataagac tctgttgcct
    13921 tgccaggacc aatacaaact gcttcatctg ccaaaagcgt atgaagagct tccttatcag
    13981 cagttgaata aaccgctacc gtcgcaatcc ccaattcacg tgccgcacgg ataatacgaa
    14041 ccgcaatttc accacgattg gcaattaaaa tttttcgaaa catggagaac ctccttagtt
    14101 cccaattgca aaagtaagga taccactggc tgcaagcttg ccatccactt cagcctttgc
    14161 ttcaaccaca gctatggtgc cacgacgttt tacaaaagtc gctgtcataa ccaattggtc
    14221 gcctggtaca acttgcttct tgaacttaac cttgtccata ccagcgtaaa agaccagttt
    14281 tcctttattt tcaggttttg ataactccaa cacaccagca gtttgcgcca aggcttccat
    14341 aatcagaaca cctggcataa ctgggtattg aggaaagtgg ccgttaaaga aaggttcgtt
    14401 aatggtcaca tttttgatag caacaatggt atcctcgctc acttccaaga cacggtccac
    14461 tagaagcata ggataacggt ggggaagagc ttctttgatt ccttgaatat cgatcatttg
    14521 atacgtacca atcctttacc aaactcaacc atttcttcgt tagagacgag aatttccgtt
    14581 accacaccat ccttaggagc tgggatttca ttcatgactt tcatggcttc gataattacc
    14641 aatgtttgac cttttttgac actatcacca actgtaacaa aggcaggttt atctggtcca
    14701 gcagccaagt aaaccactcc aacaagtgga ctctctacaa gatttccctc agtagccaca
    14761 cttgcttcag ctggagctgg aacttcttct gctacagtct ctgctggagc agatgtagga
    14821 gctactggac tcggtgttgc tagaacgggt gctggagcga cttgagttgc aacttcaggc
    14881 acaggtcttg cttcattctt gctaaactgc aactcatccg tcccattttt ataagaaaat
    14941 tctctcaaac ttgactggtc aaattgagtc atcaagtctt taatatcgtt taaattcata
    15001 cttatctatt ctcccaacgt ttgaaagcaa gaactgcatt gtggcctcca aaaccaaaag
    15061 tatttgaaat agcgtatgga atttctttct ccaagccttg tccataaacg acattagctt
    15121 cgatataatc tgatacttca cttgtcccag ctgtcattgg tacaaagtta tggcgcatag
    15181 cttcgatggt gacgatagct tctactgcac ccgcagcccc cagcaaatgt cctgtaaaag
    15241 acttggttga tgatacaggt acttccttac caagaacagc tacgatagct ccactttctc
    15301 ctttttcatt ggcaggagtt gacgttccgt gagcattgac ataggctact tgctctggag
    15361 aaatctcagc ttcttccaag gctagtttga tggccttgat agctccctga ccttctggat
    15421 gtggagaagt catgtggtag gcatcacaag tatttccgta accaaccact tcagccagga
    15481 tagtagctcc acgtttttca gcgtgttcaa gactttctaa aaccaacatc cctgaacctt
    15541 cacccataac aaacccattg cgatccttat caaatgggat cgaagcacga gttggatcct
    15601 ctgtagtaga gagagctgtt aaggcttgga aaccagcgat ggcaaaaggt gtgatagaag
    15661 cttctgttcc tcccaccaac atcacatctt ggaaaccaaa cttaatggag cggaaggcat
    15721 ccccaatcgc atcatttgat gaagagcagg cagtattgat agatttacaa acaccgtttg
    15781 caccaaaacg catggctaca ttcccagaag ccatatttgg taaagctttt ggaagagtca
    15841 ttggtttgac acgtttgggt cctttttcat gaaggcgaag tacctgatct tcaatttcct
    15901 tgattccacc aataccagat gcaacgataa caccaaaacg atccctatta agagcctcta
    15961 catcaagatt ggcatgattt acagcctctt gggctgcata caaggcatat aaagaatagt
    16021 tatcaaaacg gttggtatct ttttttacaa agtatttatc gaacggaaaa tcttggattt
    16081 ctgccgcatt atgcacatca aagtcactat gatcaaattt tgtaatgcca ccaatgccga
    16141 ttttcccagt tgctaaacta ttccaaaatt cttctggtgt atttccgatt ggagatgtta
    16201 ctccataacc tgttaccact actcgattta gtttcattct tttcacctct agctttcgct
    16261 acatacttaa gccaccatca atggcaatca cttgaccagt tagataatct tggcctgcta
    16321 aaaatactgt caaatctgca acctgctctg cctgcccaaa ttctttcatc ggaatctgag
    16381 ctagtgtagc ttccttaatc ttatctgata agatagcggt catatcagac tcaatcattc
    16441 ctggagcaat cacattgact cgtatattcc gactagcgac ctcgcgtgcc acagacttgg
    16501 taaagccaat caagccagcc ttagaagcag catagttagc ttgaccaata ttccccatca
    16561 aaccaacaac actagacata ttaatgatag caccttctct ggctttcatc atcggtttca
    16621 agactgattg tgtcatatta aaggcaccag tcagattgac cttgagcact ttttcaaaat
    16681 ctgcttctgt catcttgagc ataagagtat cttgggtaat ccctgcattg ttgaccaaaa
    16741 catctactga acccagttct gcaatagctt gatcaatcat acgcttagcg tctgcaaaat
    16801 ctgatacatc tcctgaaatg ggaaccacct tgataccata gtttgaaaac tcagcgagca
    16861 attcttctga gattgcccca cgactgttta agacaatgtt ggctcctgct tgagcaaact
    16921 tgtgggcgat ggcaagacca attccacgac tcgaacctgt aataaagata tttttatgtt
    16981 ctagtttcat ttttttcctt tcaaaacttc tacttatttt agtctatttt tctaaaagtg
    17041 ctactaaact cgcttgatct tccacatgag ctaagtgagc agtttgatca atttttttaa
    17101 caaaacctga caagactttc cccggtccaa tctcgataaa gttgcttatg cctgcttctt
    17161 gcatgacccc aatactttca tagaaacgaa cgggttcctt gacctgacgc gtcaagagct
    17221 gagcaatgtc ctctttttgc atcacagcag cttctgtatt gccgactagg ggacaagtaa
    17281 aatctgaaaa acttacctga gctagagttt cagctagttt ctggctagct ggctcaagga
    17341 gagcggtgtg aaagggacct gacaccttaa gaggaatcaa gcgtttggca cctgcttctt
    17401 gcaaaagttc aaccgctcga tcaactgcaa ccacttctcc agcaatgacg atttgtgcag
    17461 gtgtgttata gttggctgga gtaaccactc caagttcaga agctttttga caggcttctt
    17521 caatgacctc tactggcgta ttgagaactg ctaccatctt gccagagtca gcaggagtcg
    17581 cttcttccat ataggctcca cgcttagcta ccaaggcaac cgcatcttca aaatccaagg
    17641 cgccgcttgc caccaaggca gagtattctc caagagacaa accagcaacc atatcaggct
    17701 gatagccctt ttcttgcaat aaacggtaga tagcaaccga agtcgctaga atggctggtt
    17761 gcgtatagcg ggtctgattg agtttgtcct cttccgtatc gatgagataa cgcaaatcat
    17821 aaccgagcac ctgactcgct cgatcaatcg tttctttaac aatcggatac tgatcataga
    17881 aatcccgtcc catccctaga tactgggcac cttgaccagc aaataaaaag gctgttttag
    17941 tcatttctta caactcctgt ccagcgagag gcttcttctt gaattttctt agcggctccg
    18001 taatacaaat cttttaggat ttcttcagct gtttcttctt tagaaacaag ccctgcgatt
    18061 tgacctgcca tgacagagcc accatccaca tcaccgtgaa caactgcttt ggctagggca
    18121 cctgctccca tttgttcaaa gatttctaaa tcaggatctt cctgcttaaa ggcatctttt
    18181 tcagccagtt caaaatctct agtcaactga tttttaatag cacgaacagc atgaccaaag
    18241 tgctgagctg aaatcgtagt gtcaatatcc cttgctttta aaattttctc cttgtagttt
    18301 ggatgggcat tcgactcttt tgcaactaca aaccgtgtcc ccacctgtac agcctctgca
    18361 cctagcataa agccagccgc agcaccttca ccatccgcaa ttcctcctgc agcaataaca
    18421 ggaatagata tagctgtggc tacctgtcgc accaaggtca tggttgttaa tttgccgata
    18481 tgccccccag cttccattcc ttctgcaata acagcgtctg caccgatttt ttccatgcgt
    18541 ttagctaaag cgacactagg aacaacagga ataacgatta tcccagcttc atggaaacgt
    18601 tccatatact tgcttggatt tcctgctcct gttgtgacaa ctttaacacc ttcttcaata
    18661 acgagatcca cgatatcttc cacaaaggga gataagagca tgatgttgac cccaaagggt
    18721 ttatcagtca atgatttgat tttatcaata ttggccttga caacttcttt cggggcattt
    18781 cccccaccga taattcctaa tcctccagcc ttggaaacag cccctgccaa atcaccatca
    18841 gcaacccagg ccatccctcc ttggaaaata ggatagtcaa tcttcaataa ttctgtaata
    18901 cgcgttttca tagtgcctcc aaccttcctt gcttacgtaa tagttcgatt tcaccataat
    18961 ttgacagtca aactattacc taaacaagag ggagtgggtt tctccctact ccttctacta
    19021 atattctgct tattttgctt gctcttcaac gtaagcaacc aagtcaccaa ctgttttcaa
    19081 gtcattttct gcttcgattt ggatatcaaa agcatcttcg atttctgaga ttacttggaa
    19141 caagtccaat gaatctgcgt ccaaatcatc aaaagttgat tcaagtgtta cttctgatgc
    19201 gtcttttcca agttcttcaa cgataatttc ttgtactttt tcaaatactg ccatgatagg
    19261 actcctttaa aataaatagt ttttttataa caatgtgttc accacatgat tacctaaatt
    19321 gtaagaatga gcgtgcccca ggtcaagcct ccaccgaagc ctgatagaag aacagtctgg
    19381 ctaccatcta aagggatgag accttgttct acacactctg aaagtaaaat cgggatactg
    19441 gctgcactgg tattgccata ttccatcata ttggctggaa gtttggctcg gtcaacacca
    19501 atttttctag ccatcttatc caaaatacgg tcattggctt gatgaagtag cagataatcc
    19561 aagtctgtca cctctatagg agattcatca atagtctgct tgatagactt ggctacatct
    19621 cgaatggcaa aatcaaagac tgtgcgtcca tccatcttca aaaacgaatc tgcactttct
    19681 tgatctgaaa atggagaatg caaacctgaa tgcccataag ttaaacactc gctgcgactt
    19741 ccatcgctat tgagactctc agctaagaaa tgctcttgct cgctagcttc taacaagaca
    19801 ccaccagcac catctccaaa caacacagct gttgatcgat ccgaccaatc gactgcctta
    19861 gagagggttt cactaccaat caccaagcct ttttgaaagc gaccagaagc gataaacttt
    19921 tcagcagttg aaagagcaaa tacaaatcca ctgcaagccg cggttaagtc aaaagcaaag
    19981 gctttattag cgccaatatt agcttgaaca cgagcagctg tagagggcat catcgaatct
    20041 ggagtaatgg tagctaggat gataaaatcc agttcttttc ctgttattcc agcttttgcc
    20101 atcagtttct tagcaacctc tgtagccaaa tcactggtag attctgttct tgaaatatgc
    20161 ctttgtcgta ttcccgttcg acttgaaatc cactcatcat tggtatccat aatctgagcc
    20221 aagtcgtgat ttgtaaccac ttgctctggc acataatgag caacctgact tatttttgca
    20281 aaagccatta tttcaaatcc tccaaaaatt ggtaaagatt agtcaagcct ttacccatga
    20341 cagcaatttc ttcctcgctc atgccatcaa taattttttc taccatggcc ttgtggaagc
    20401 gtttatgcag tctatgaatc aagcgaccct tctttgtcaa atgcagatgc accacacgac
    20461 gatcctgttc tgaccgaact cgctcaatgt agcccttacg ctctaaattg ttcaaacttg
    20521 tcgtaacagt tccaagagtt accatcaact cttttgacac ttgacttgga gtcacatctg
    20581 gagccttacc gatgacatca atcgtatgca tttctttgat ggagatatcc ttaaaacgac
    20641 tacctctcaa gttcacttcc tcaattacaa ggacattgtt aaatatagat gttaaatatt
    20701 cattgattcg ttggtagtcc atttttcata tccctccttc ttcaaaaaaa cttttacaat
    20761 caaaacattt gacagaaaga attatatcaa aattcaaaga atgatgcaag tattttttat
    20821 tttcctgtaa atttaggtct tcttctctcc gaatgagctc gcactccttc tttgaaatcc
    20881 tctgtttgag ctaaggattt ctgtaggttc agttctaaag tagcgtaacc ctgccagtct
    20941 ttaaattggc tctcccaaac caacttctta atggcagcat aggaattact tgaagcacgt
    21001 cttaattttt taagaagctg ttctctcgtc ttttcaagtt tttcagcttc agaaacgcgg
    21061 taaaccaacc cccattctag ggctttttct gccgttaaag cctctcctgt catagctaat
    21121 tgagcagcac gcgtcacacc gatgctgcgg ctcaagagat gaattccccc tgcatctgga
    21181 gccaaaccaa caccaacaaa ggcttggata aacttagcct tatccgtcgc caaacagaaa
    21241 tctgccgcaa cagccatatt cgctgcggca cctgcaacag caccgtcaac ttccattaaa
    21301 acaggtttgg ctatttgctt gattttataa gaaatcgtat tgaccaactc tgcgattttt
    21361 gtcaatgatg gaatatcatc ctcatccact gctcgcttca tctctaccaa atctccccca
    21421 actgagaaga cctttccatt ggcattgatt aagataaaat gcacagctgg attttcttct
    21481 gccaaagtca gagcttctaa aatctcctca cacatgggaa tatgaaaacc atttgcgacc
    21541 tcaggacggt tcaaggtaag gattgccaag tcctcttcaa gctgataaat aatgtgttcc
    21601 atcagggctc ctttttattt tataagcagt ttatactctt cgaaaatctc ttcaaaccgc
    21661 gtcagcttcc atctgcaacc tcaaagcagt gctttgagca gtctgcggct agcttcctag
    21721 tttgctcttt gattttcatt gagtgttaaa ttatttcact gtcaaagtat atctttttta
    21781 ggagcagaaa gcaagaaaaa atcgactgaa agtgtctcag ccgataagaa aggctcgctt
    21841 taggctagaa agcatcttat tttcttcatt gaaaaaagcc tttctttctg ctataatcgt
    21901 ataaaatact tactttagga gttcttatga aagttgttaa atttggaggt agttctcttg
    21961 cctctgctag tcaattagaa aaagttttaa acatcgtcaa aagcgattca gagcgtcgtt
    22021 ttgtagtcgt ttctgcgcct ggtaaacgca atgctgaaga tactaaggtt acggatgccc
    22081 tgattaaata ctaccgcgac tatgttgcgg gtaacgatat tagcaagagc caaagctgga
    22141 ttatcgaccg ctatgctgct atggttagtg aattgggact aaaaccagct gtgctagaaa
    22201 aaatttctaa aagcattcac gccttggcca ctcttcctat tgaagaaaat gaatttctct
    22261 acgatacttt cctagcagcc ggtgaaaata acaatgccaa attgattgct gcctacttta
    22321 accaaaatgg tatcgatgca cgctatatgc accctagaga agctgggatt gtggtcacaa
    22381 gtgaacctgg tcacgctcgc atcattccat caagttatga caagattgaa gaattgacaa
    22441 acaccaatga agtccttgtc attcctggtt tctttggtgt cactaaggaa aatcaaatct
    22501 gtactttctc acgtggaggt tctgatatta caggttctat cattgctgct ggtgtcaaag
    22561 ctgacctcta tgaaaacttt acggacgttg atggtatctt tgcagcccac cctggtatta
    22621 tccaccaacc acactcgatt cctgagttga cctaccgtga aatgcgcgag ttggcctatg
    22681 caggcttctc agtccttcat gacgaggctc ttcttcctgc ctaccgtgga aaaattcctc
    22741 tggttatcaa gaataccaac aaccctgacc atccaggtac tcgtatcgtt ctaaaacaca
    22801 gtaatgatga atttccagtt gtgggaattg ctggtgactc aggctttgtc agcattaaca
    22861 tgtcgaaata tctcatgaac cgtgaggttg gatttggccg caaggttctg caaatcctgg
    22921 aagaacttaa catcggttgg gaacatatgc caacaggtat cgacgatctt tctatcattc
    22981 tccgttctcg cgaactaact cccatcaagg aagaagaaat cctgcgtcag ttggttcaaa
    23041 agactaaagt agatcatgca gaaatcgaac acgacctttc tatcattatg attgttggtg
    23101 aaaaaatgaa gagccacatc ggagtgactg ctactgcgac acgcgctcta tctgaaaaca
    23161 aaatcaacat ccagatgatg tctcaaggtt ctagtgaagt ttctattatg tttgttgtca
    23221 ataaagacca agagaaagca gcaattaaag ccctctacaa tgcctttttt ggaaaaagta
    23281 aggaagacta agcatggccc aatcacttaa caaaacagta ctcctcagta cgacaggcac
    23341 ttcctacctc tctatcgctg ggaaagttgg gaaattcctt gtcggagatc aggctttgga
    23401 attttaccca gatgtcaatg tcgaacaatt tatccagatt ccttggagcc atatcaatca
    23461 gattggagcc aatgtcactg gacgcaaaat cagccgccac ttcgaagtct ttacagacaa
    23521 aggaaaattc ctctttgctt caaaagactc aggtgccatt ctcaaaattg cacgcgaaaa
    23581 actaggcaat gacaaggtcg tcaaacttcc gaccctgatt cagacaatta gccaaaaatt
    23641 taaaaatcta tttgcaaaaa agtagaaact ttagtataat catagcaacg gataagtagg
    23701 ttatcttctt ctttcagaaa gtctgcgggt actgcgagca gataggaggg ataggtgaaa
    23761 ttctaccgtt agtgacaatt acatacacaa tcaagtgcac acctgtgaag ttggatggaa
    23821 ccgtggccct gccactccaa cgttttgtca gggtgctttt ttcataaagg agttcttatg
    23881 ttagatatca aacgtattcg tacagatttt gaagctgtcg cagaaaaatt agctacacgt
    23941 ggtgtagatg ctgctgtctt gaatgaaatg aaagaaatcg atgctaaacg tcgtaacatc
    24001 ttggtcaagg ttgaaactct caaagcagaa cgtaacacag tttctgctga gattgcccaa
    24061 gctaagcgca acaaggaaaa tacagatgac aagattgctg ccatgcaaaa tctatctgct
    24121 gaggttaaag ccttggatgc tgaattggca gaaatcgatg ctaaattgac agaatttaca
    24181 acgactcttc caaatatccc agctgacagc gttcctgttg gggctgacga agacgacaat
    24241 gtggaagttc gccgttgggg tactccacgc gagtttgact tcgaacctaa agctcactgg
    24301 gatctcggtg aagaccttgg tatccttgac tgggaacgcg gtggtaaggt aacaggcgct
    24361 cgcttcctct tctataaagg cctcggtgct cgtttggaac gtgctatcta caactttatg
    24421 ttggatgaac atggaaaaga gggctatact gaagtcatca caccttacat agtcaaccat
    24481 gattctatgt ttggtactgg tcagtatcca aaatttaagg aagatacttt tgaactcagc
    24541 gataccaact ttgtcttgat tccaactgct gaagttcctc tgacaaacta ctaccgtgat
    24601 gaaatcttag acggcaaaga tcttccaatc tacttcactg ctatgagtcc gtcattccgt
    24661 tctgaggctg gttctgccgg tcgtgatacg cgtggcttga tccgtttgca ccaattccac
    24721 aaggttgaaa tggtcaaatt tgccaaacct gaagaatctt acgaagaatt ggaaaaaatg
    24781 acagccaacg ctgaaaacat tcttcaaaaa ctcaaccttc cataccgtgt cgttgctctc
    24841 tctactggag atatgggctt ctcagctacg aagacttacg acttggaagt gtggattcca
    24901 gcacaaaaca attaccgtga aatctcaagc tgttcaaaca cagaagattt ccaagcccgt
    24961 cgtgcccaaa tccgttaccg tgatgaagca gatggcaagg tgaaactcct tcataccttg
    25021 aacggttctg gacttgcagt tggacgtaca gtggctgcaa ttcttgaaaa ttaccaaaat
    25081 gaagatggtt ctgtgaccat cccagaagca cttcgtccat acatgggtgg agctgaagtc
    25141 atcaaaccat aaaaaaataa ggtttagcta tttctagcta gacctttttt cgtaaccaaa
    25201 tcagataagc acctagtaca aagaataaaa tagttaggca tataatggtt tcagtcaata
    25261 ccaggtaatc cagaaatgga agtttcaaaa ttccctgagc catcttgagc gaggtcgctg
    25321 tgataatggt tgggaaggtg agggctgaga aggctggttg aaaaccttgt tttaaaatgt
    25381 tgggcagacg agttaaaaca aagaaaaaga aggattgaga agccaaaatc atgacaatca
    25441 agacccaagt cggcaggctg gttcctccta ctcgaactag agaagccaag agtagagaga
    25501 aaggagcaca gtagattcct tcttgtccaa gcaaggctag tgggagtgga tgtttcttta
    25561 aatcgctata aataagggga tagagataga aggtcaagag aaaaccaaaa ctcaaggtcg
    25621 cataggcaat ttcgataata cctaccagag gataggtcaa ggcagccact gctatcccca
    25681 catagagaac cgtccaactt ggagtggcat gaaccctccg ccctggacaa gcaaacttga
    25741 tggtaaaacc agcaatcaag gtcaaatcca agagaaatga aaaccaccaa atcccttggg
    25801 ctaccaaagg aagataagag aatacgcgaa agacataggt cgataaaatc atcccagcca
    25861 taggaaaggt tgccattcct gacaaaagag ggggcttggt caattcttgc ttggtttctt
    25921 tccaattaaa gagatgcaga attagaaagt aaatccataa aaccaaacca gtcagactaa
    25981 aaagatggga tagaaccggc aacgtatcta aaataagatt tccagctcct gccaaaccta
    26041 gcaaacaacc tgaaaatact aaggggagtt ttttcatcct aacctccaat aatcatgtta
    26101 gtttcagtat aacataaaag cgcttaaatg aggatttaaa aaacgaagtc cgcttatttc
    26161 agacttcatt ttactcagat atgaattagg cataaggttg caattctgga ttaattggtg
    26221 tattagctaa gttgttggca tagttacaga ggattgctag gctgacacca aaaaccacat
    26281 ccaaggcatt ttgttgagtg tagccagctt ctaaaaactc agacaaggct tcatctccta
    26341 cacgaccctt ggtattgata actgccaagg taaacttagc tagggtatcc aatttaggat
    26401 ctgtttcaat tggagtacga ttgcgaagag cttgaatcaa gtcatcattc atctggattt
    26461 gtttgatgga aaaggctgtg tgacctgcga cacagaaggc acaaccattg gtcacggctg
    26521 ccgtgatttg caccacttca cgctcaacgg gtgtcaggct gttgcgacgg tggatagatg
    26581 agacaatttg gtaggcttct aaaacagtcg gggcattggc caagagaccg attaggttgg
    26641 gaatatagcc attgttgtct ttttctactg tttcaagaat ttctttcact tctgctggtg
    26701 ctgactctac tgtatggata gtaaatgttg tcataagata cctcttttct tattattgac
    26761 actaatatta ttggaaaatc ttataaaatc ctgattccta agtttatcta agataaagct
    26821 ttattctctc ataagatttt cgttgttata ttagtttatc acacttccaa tcacttgcat
    26881 aatatatatt atatatcagg ctgataaaaa ttatttatag gcaaaaaaat cacacgagct
    26941 gtgtgattcc attatttgtc aaaatacttt ttagtttcag caataacgac tggcgacaag
    27001 accaagaggg caatcaagtt tggcagagcc atcaaggcgt taacgatatc tgcgataatc
    27061 cagaccatat ccaactcgat aaatcctcct aacaagacca tgagcacaaa aaccacacgg
    27121 tagagccaga taaagcgaac cccaaagagg aactcaaaac agcgttctcc gtaatagttc
    27181 caacctagaa tcgttgtaaa ggcaaaaagt acaaggaaga tggtcaagag ggcaggccca
    27241 aagtgtgaaa agactgttga gaaagctgac tgagtcaagg caaccccatt caagtcacca
    27301 ctccaaactc cagttaccaa gatggtcaaa ccagttagag tacaaatgat gagggtatca
    27361 ataaaggttc ctgtcatgga aatcaaacct tgctctactg gttcatttgt cttggcagct
    27421 gcagctgcaa taggagcaga acccagacca gattcgtttg agaacacacc acgcgccaca
    27481 ccattttgaa tagccatccg aacgctagca ccagcaaatc cacctaccgc agcaagggga
    27541 ctaaaagctg aggtaaagac taaagcgatt gtgccaggga tttttccgat attaaagaaa
    27601 ataactgtaa gagttcctaa gatataaatg agggccataa aaggaacaac agtagttgaa
    27661 accttagaaa tagacttgag tccaccaaag actgcaatcg ctacaaagac agacaagacg
    27721 agagctgtga tggctggcga aatcgtcgtt gtattttgga tagattctgt aatcgagttg
    27781 acttgggtga aggttccgat tcccaagaga gcaaccaata ctcctgctac tgcaaacaaa
    27841 acagcaagtg gtcgccactt ttctcccatc cctagaagga tatagtgcat gggacctccc
    27901 gctactgcac catggtcgtc cttggtgcgg tatttgatgg ccaagagtcc ttccgcatac
    27961 ttggtagcca ttccaaagaa agccgccatc cacatccaaa atagagctcc tggtccacca
    28021 accttgatag ccgtcgcaac tcctatgata tttcctgttc caacagttga tgccaaggct
    28081 gtacacagag ctgcaaaact ggatacatca ccatgtccct tatcctggat aaaaataagc
    28141 tgaaaggcct tgggcagacg caaaacctgc aagagtccta gccgcatggt taggtaaatc
    28201 cctgttccga ccaataaaat caagaggggc ggtccccaag caaaagcatc gattgattta
    28261 agcaattcta acatttcctt ctcctatcgt ttcaacccca aaagaaagag cacatgcaag
    28321 acacatgtac tctggaatgc ttagataaat gctaaaaagc ggtctatcct agctctgtcc
    28381 ttttacctga gagtttgagc agttgcctgc cttgcccctt cggtgccttt acggtctctc
    28441 cagagttccg tccatttaca gtcatggaaa atcaaacgat tccccacttc tattaaactt
    28501 cattcggtgt tggtatttaa ttgattctaa ttttacaaaa aatgttggct tttgtcaatg
    28561 tgtttattag taaaaattag ttcaacagtt tttactttat aaagtccaga atactgctat
    28621 cctttaaaag tgacaatagt cgcaccactg cctccagcat tttgtggggc atagccgaaa
    28681 ctcttgacat gtttgtttct ttgcaagtat ttggtaactc cttcacggat gactcctgtt
    28741 ccgataccat ggatgatatc aacttgagcc atattgttaa gcaaggcttg gtcgatgaag
    28801 gcatctagct cattcatggc ttcttcatag cgcttgcctc gaagatccag tctagcttga
    28861 ggtcctcgcc cagaagttcg tttcacaaca ttgacctgtt tcttcttgac tggtttttct
    28921 tgctgggctt gaacaagatc aaactctttc tcttccaagg tcatcttaat caagccaact
    28981 tgggcttccc agcgaccgtc cttgagttga ctggtcaagg taccacgctg accataactg
    29041 agaaccacga tatcatctcc cacctttgga gctcgttttt tcttggcctt ttgaaggacc
    29101 ttatttttag acaagtccac tttttcagga gccaattttt tcaacttggc cttggcttca
    29161 atgatttcgt ggggcttgag ttgggattta ctgtggagat ttttgagaat ctggtcactt
    29221 tcacttaggg ccatatccac aatctcagca gcctgttcac gcgccttgtt aagctcgatt
    29281 tccttttcac gattaagctc gttgtagagt ttttttagcg cacggttcat cttgagattt
    29341 tcttgctcca cctcacggat attgtccaaa cgtttgcggc tttccagcgt ctgctcttct
    29401 aattgctcaa tgatacgatt gacgtcattg tcctgatcga tctgctgact ggcatctcct
    29461 acgataactt cagataggcc tagacgtttg gcaatttcaa aggcattact tcggccagga
    29521 acaccctgca taaagcgata ggtcgggcga agagttgcag tatcaaactc catactggca
    29581 ttttgcacaa aggctgtctc aataccgtag gccttgagtt ctggatagtg ggtcgtcgcc
    29641 atggtcttga tttgacgcag gcgaaggtcc tccagaatag ccatggcaag ggctgctccc
    29701 tcttggggat cagtaccagc ccccaactca tccaaaagta agagtgaatg ttggttgacc
    29761 ttgccaagaa tatccacgat attggtcata tggctagaga aggtagacaa gctctgctca
    29821 atagactgct catctccaat atcagcaaag atttcttcaa aaataccaac acgacttccc
    29881 ttgtctgcta aaatcggcaa tcctgactgg gccatgacct gtgtcaagcc cagagttttg
    29941 agcatgatgg tcttcccacc tgtattggga cctgtaatga caatagctgt taaatcttga
    30001 ccaaaatgga catcatttgc gacggcattt ttgaccaaag gatggcagac atggagcagt
    30061 tgaatctctt gattttctga cagctgaggc acgactgctt gtctttcttg gataaatcga
    30121 accttggcac gaatcaagtc cagatgaccg ataatccaag catcattagc aatctcagcc
    30181 gcatgagggc ggacacgctc agaaatttct tggagaatgc gaagcatttc atagcgctca
    30241 tctgctcgca gactagcaat ttcttcgctc agtttgacta cctcacgtgg ttcgatatag
    30301 acggtgtttc cactagcaga aatatcatga acgacacctg caatcttatt gcggtaggtg
    30361 tttttgactg gtaaaacctg acggccattt ctgctagcaa caattccttc cgtcaacatc
    30421 tgcgcttttt gcttgagcaa gtcttgtaaa acatcgcgta cctgactctc gctatcatgt
    30481 atttttcgac ggattcgcgc caattcttca ctggcaaaat tttcaatgaa acccgcatca
    30541 ttaaaggcct gaagatttcc ttgtaattgc ggaaaatcat gtaatttctc aaaccaaagg
    30601 gctaattctt ccaagctgac attttccaga ttggcgtaaa aactttgaag ttctcggctg
    30661 gcaagaagca cgcgtttcaa gagtaggaac tcctcgatat tgagatccgc tcccatctcc
    30721 aacctcttgc agactcctgc aatttcctta gttgagagaa tagtaaaatg cggttgctcg
    30781 acgaaaagag cctgcatttc tttcatctca gcaaaagcct gtttgatttt atctgctttg
    30841 gcagtcggag ccagctgtct caattgctcc aagccctgct cggtcaacaa atgaggctca
    30901 aacaaggcct tgaccttatc gaactctaat gtttctaata ttttcttatt catctttatt
    30961 tccttgtctt tgaatgtcta ggtgtggtag ctttttacat tctgatagat tcgagtcaaa
    31021 tctgtaaaca aagggctagg aaaactcctg ccctttttat ccgattaaat ttgtcaccca
    31081 gatttgtttg agccaactgg ttgttatcgg tatgctctgg atgatgtgtt ttgcgacgat
    31141 actcttttca agaggatttt gtataactgc catggggatg gtcgccaaga ttgtcaaggc
    31201 catttgcaag acaaataagg tcaccaacat ggacaagata cctgctgaaa cttggaacaa
    31261 cttaccaccc agttttttgc taggaatcaa gtgtaagaga agaccaagca aacgaccaat
    31321 gctatagaca atcccaaata caagcaagta gccgatacct gcataaaaga ccttatccag
    31381 ctgaaagagt tgatccgatg ggaaaaagaa agtcccctga ccttcctgcg aatttgcata
    31441 agggagcaat aaatggaatt gctctccaag ccccttataa aactggccag ccataaaagc
    31501 cgatgccatg gctgaaatca ggtaataaac ctgtaagagc aggcctctcc gatagccgat
    31561 ataaaatccc caaaccaaga ccaatagaag aaggagtgaa atcataagga atcctcaatc
    31621 ttgctctgtt cttgcttaca agtcacaagc ttgtgacgga gttcttctag ctcttgctcc
    31681 ttatcgtcaa attcaatctc acggctgagc tgagttgata aacagttgac tgccaacaaa
    31741 agagcgattg tttcatcatc tgcgctaggc atttgttctt taattgcttg gtatttttct
    31801 gtcgcaacct tagcgatttc ctccataaaa aggttatcat gttcgcttgt caaggttaac
    31861 gattttttcc cgaatgtaaa tttgaatcga tttagatttg ccataaaatt cacctcacga
    31921 tattatacca aaattcgcta attttgtcag tttttacaaa ttttactgct tttgtggtac
    31981 aatagaaact atggcaagta tcacactcac accaagcgaa aaggatattc aggcttttct
    32041 tgaacactat caaactagtc tggctcctag caagaatccc tatatccgct actttttgaa
    32101 actacctcaa gcaacggttt ctatctatac ttctggaaaa atcttgcttc agggtgaagg
    32161 ggctgaaaaa tacgccagtt tctttggcta tcaagctgta gagcaaacca gcggacaaaa
    32221 tcttccttta attgggacag atgaggtggg aaatggttcc tactttggtg ggcttgcagt
    32281 cgtggctgcc tttgtcacac ctgaccagca cgacttttta cgaaaactcg gtgtgggaga
    32341 ttctaagact ctgaccgacc aaaagattcg tcagattgct cctattctca aggaaaaaat
    32401 ccagcaccag gcactccttc tctcacccag caagtacaac gaggtcatcg gagaccgcta
    32461 caatgctgtt tcggttaagg ttgccctcca taatcaggct atctatctcc tccttcaaaa
    32521 aggtgttcag cctgagaaaa ttgtgattga tgcctttacc agtgctaaaa attatgacaa
    32581 gtacttggca caagaggcca atcgtttcag caatcctatc agcttagaag aaaaggctga
    32641 gggcaaatac ttgtctgtcg cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa
    32701 tcttgaaaat ctgggacgag aactgggtta tcagcttcca agtggagctg gaacggcttc
    32761 tgacaaggtg gctagccaga ttttgcaagc ctatggtatg cagggactca acttctgcgc
    32821 caaattgcac tttaaaaaca ctgaaaaagc gaaaaaacgc ttagaaaggt aagttatgaa
    32881 ttcatttaaa aatttcttaa aagagtgggg actgttcctc ctaattctgt cattactagc
    32941 tttaagtcgt atcttttttt ggagcaatgt tcgcgtagaa ggacattcca tggatccgac
    33001 cctagcggat ggtgaaatcc tctttgttgt taagcacctc cctattgacc gttttgatat
    33061 cgtggtggcc catgaggaag atggcaataa ggacatcgtc aagcgcgtga ttggaatgcc
    33121 tggcgacacc attcgttacg aaaatgataa actctacatc aatgacaaag aaacggacga
    33181 gccttatcta gcagactata tcaaacgatt caaggatgac aaactccaaa gcacttactc
    33241 aggcaagggc tttgaaggaa ataaaggaac tttctttaga agtatcgctc aaaaagccca
    33301 agccttcaca gttgatgtca actacaacac caactttagc tttactgttc cagaaggaga
    33361 ataccttctc ctcggagatg accgcttggt ttcgagcgac agccgccacg taggtacctt
    33421 caaagcaaaa gatatcacag gggaagctaa attccgcttc tggccaatca cccgtatcgg
    33481 aacattttaa gaaacctaag aggccgagaa tcaccaatct cagcctcttc ttctatcgtg
    33541 agaaaatgat tggtaactat ctaaacttac cagaacagaa acacctcaac tctcacctat
    33601 tcatgcaaag gaattttatg gaagtttatt tttcaggaac cattgaacgg attatttttg
    33661 aaaatcccag caatttttat cgcatcctcc tcctagaaat cgacgatacg gacgcagagg
    33721 attttgatga ttttgaaatc attgtcacag gaaccatggc tgatgtaatt gagggcgaag
    33781 actatacttt ttgggggcaa attgtccagc actccaagta tggagaacaa ctgcaaatca
    33841 gtcgttatga tcgcgcaaaa ccaactagta agggcttggt caagtacttt tcaagtagcc
    33901 atttcaaggg gattggtctc aagacagctc agaaaatcgt ggatacctat ggcgaaaata
    33961 ccattgacga aattttgcaa cacccagaaa agttagaagg catcgcagga ctctctgcca
    34021 aaaatcgcga ggctttcgtc tccactctcc gtctcaacta cggaacggag atggttttgt
    34081 ccaaactagc caactacggc attcccaaca aactagcctt tcagattcaa gacttttaca
    34141 aggaagaaac ccttgatgtg gttgaaaatt atccctacca gctggttgag gatatcaagg
    34201 gtttgggctt tacaattgct gaccaactag ctgaggaact aggcatcgaa agtcaggctc
    34261 ctgaacgctt ccgcgccggt ctagttcaca gtctttttca ggcctgtatg gaaacagggg
    34321 acacctatgt tgaagcacgg gatttgctgg aacaaaccct tactctcctt gagtcttccc
    34381 gtcccgtaga actagacccc agccaagttg cccaagagct ctcctacctg atcgaagaag
    34441 acaaggttca gcagattgat accaagatct ttgacaacag cctctttttc gctgaggaag
    34501 gtatccgcag tcacttgatt cgtatccttg aaaaaggaaa acagaagagc caagatttag
    34561 aaactattca aaaacatatc actactgtcg agcaagaact ggggattgag tatgataaca
    34621 ttcaaaaaca agctatttgt gacgctatcc agaacaaggt ctttatcctg acaggtgggc
    34681 ctggtactgg taagacaact gttatcaatg gaatcatcgc tgtttatgcc cttttagaag
    34741 gacttgactt caggaagaaa agcaatctgc ccattcttct tgctgctcca actggtcgag
    34801 ctgctcgtcg catgaatgaa ttgacaggtt tgcctagcgc gaccatacac cgccacttgg
    34861 gaatgacagg tgacgatgat accagtcatc tggaagatta cctagatgct gactttatca
    34921 tcgtggatga attctctatg gtggatactt ggctggccaa tcaactcttc tccaatatct
    34981 cttctaacag taagatcctc atcgtaggtg acagtgatca gctaccttct gtcagtcctg
    35041 gacaggttct agcggatctg cttcatattc ctttgattcc tcagactcgc ttggaaaaaa
    35101 tttatcgaca aagcgaagaa tcaaccatcg tcaccctagc tagtcagatt cgacagggca
    35161 tcttgccagt tgatttcacc caaaaaaaag ctgaccgttc ctactttgaa attactagtg
    35221 gccatattcc tgccaccatt gaaaaaatct taggtgccgc cctcagaaat ggtattcctg
    35281 cccgtgatat ccaagttctg gctcctatgt accgagggac ggcagggatt gatgctatca
    35341 atcagctcat gcaagacctg ctcaatcccc cacaaaaaga tcaactcagt tttgaagctc
    35401 ctcagtgcca ctatcgtaag ggagacaagg tcattcattt ggttaacgat gctgaaatca
    35461 atgtctttaa tggggattta ggagctatca cagacctgat tcctggtaaa tacaccgaat
    35521 cgaaacaaga cgagattgtc attgattttg atggtaatga agtctcttac ccccgtaacg
    35581 aatggtacaa gattcgcttg gcctatgcca tgagtattca taagtctcag ggaagtgagt
    35641 tcccagttgt catcctaccg attactagtg ctagccgacg tatgctggag cgaaatctca
    35701 tctacacagc cattacacgt gccaaaagca aactcatctt actaggcgaa ttacaagcct
    35761 tcgactatgc tacccaacac atcggaactg cccgaaaaac ctatctgatt gaacgcttca
    35821 gtgatctact ggagaatgtt gaagaaaagc aacaagctgt ctctgaaaca gtcacatcaa
    35881 gtgcctctga acaatcctac atcctaaccg aagaaaactg ggacagcatt ccagccatga
    35941 ttgggattac agacacagac ctcaaagaga tttttggaaa atagtgcatc agaaaaacca
    36001 ccaattcagg tgggttttct gtttattaag attattttac tgttgctgtg cttgtaatca
    36061 attcaacagc ttttttgata gtgatatcat gtttcaacat gtcagctgaa agcaagtttt
    36121 gaacttgtgc aacttccatg ttgtagtctg ctgccaattg ctcaacttct ttttggattt
    36181 cttcttctga agcatcaaat ccttcagctt tggcaactgc ttcgataaca aggttagtct
    36241 tagtacgtga ctcagcttct gcttggtatt ggttgtgaag gtcttcttga gtagttccag
    36301 taatttggaa gtacatgtca gggttgatcc cttgacgttg caagttccca aggaattcat
    36361 ttactgaacg gtgaacttct tcgtggatca tttcttctgg aagttctacg atttcagcat
    36421 tttctacagc tgtatcaatt gctgcacctt caactgcatc tttgtaagct tcttctttag
    36481 cagcagccaa ttctttgcgg tatttttctt tcaagtcagc aagtgtttca acttcttcat
    36541 caatgtcttt tgcaagttca tcgtcaagag ctggaacttc tttagctttt acttcgtgga
    36601 tagttgtcac gaatttagct tctttacctg caaggtcttc tgcttggtag tcttctggga
    36661 atgttacgat aacatcaacg gtttcgccag ctgagtgacc taccaattgg tcttcgaaac
    36721 cagggatgaa ttgacctgaa ccaagtccaa gtgagaagtt ttcacctttt ccaccgtcaa
    36781 attcaacacc gtcgatagaa ccaacgaagt cgatcacaac agtgtcgccg tcttcagcag
    36841 cagcttcctt gataaccaat tcagccaggt tgttgcgttc gcgttcgata cgctcttcga
    36901 catcagcgtc agttacttct ttttctacat caactgatac ttcaaggttt ttgtagtcac
    36961 ccaattttac ttcaggtttt gtaacaactt cagcagcgat aacccagtct tgaccttttt
    37021 ccattgaagt tacgtcaatt tttggttggg caaccacttc aagaccagct tcttttacag
    37081 ctgcttcata agcgtttggc aaaagagcgt tcataacgtc ttggtaaagt gattcttcac
    37141 caaatttttt atcgaagata ggacgtggaa ggtgaccttt acggaaacct ggaacattaa
    37201 gagatttctt cactgacttg aagacacggt ccaattctgg tttgatttgg tcttgagaga
    37261 tagtgaaagt caagacacca cggtttgttt ctttgttttc aaatgataca gacattctgt
    37321 catttctcct taaaattttt taaatacagt ctattataac attttctata aataaaatct
    37381 agactaatgc agacaattat agacaacaag cctttttcct aact
//
LOCUS       DAVKJU010000015        37420 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVKJU010000015 DAVKJU010000000
VERSION     DAVKJU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.015"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            2..74
                     /locus_tag="VW191_000648"
     tRNA            2..74
                     /locus_tag="VW191_000648"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            99..171
                     /locus_tag="VW191_000649"
     tRNA            99..171
                     /locus_tag="VW191_000649"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:132..134,aa:Lys,seq:ttt)
     gene            177..258
                     /locus_tag="VW191_000650"
     tRNA            177..258
                     /locus_tag="VW191_000650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:211..213,aa:Leu,seq:tag)
     gene            277..349
                     /locus_tag="VW191_000651"
     tRNA            277..349
                     /locus_tag="VW191_000651"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Thr,seq:tgt)
     gene            372..443
                     /locus_tag="VW191_000652"
     tRNA            372..443
                     /locus_tag="VW191_000652"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Gly,seq:gcc)
     gene            451..536
                     /locus_tag="VW191_000653"
     tRNA            451..536
                     /locus_tag="VW191_000653"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:485..487,aa:Leu,seq:taa)
     gene            546..619
                     /locus_tag="VW191_000654"
     tRNA            546..619
                     /locus_tag="VW191_000654"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:580..582,aa:Arg,seq:acg)
     gene            635..708
                     /locus_tag="VW191_000655"
     tRNA            635..708
                     /locus_tag="VW191_000655"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:669..671,aa:Pro,seq:tgg)
     gene            714..787
                     /locus_tag="VW191_000656"
     tRNA            714..787
                     /locus_tag="VW191_000656"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Met,seq:cat)
     gene            801..874
                     /locus_tag="VW191_000657"
     tRNA            801..874
                     /locus_tag="VW191_000657"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:835..837,aa:Ile,seq:cat)
     gene            885..974
                     /locus_tag="VW191_000658"
     tRNA            885..974
                     /locus_tag="VW191_000658"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ser,seq:tga)
     gene            985..1058
                     /locus_tag="VW191_000659"
     tRNA            985..1058
                     /locus_tag="VW191_000659"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1019..1021,aa:Met,seq:cat)
     gene            1062..1134
                     /locus_tag="VW191_000660"
     tRNA            1062..1134
                     /locus_tag="VW191_000660"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Phe,seq:gaa)
     gene            1154..1224
                     /locus_tag="VW191_000661"
     tRNA            1154..1224
                     /locus_tag="VW191_000661"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Gly,seq:tcc)
     gene            1260..1333
                     /locus_tag="VW191_000662"
     tRNA            1260..1333
                     /locus_tag="VW191_000662"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1294..1296,aa:Ile,seq:gat)
     gene            1340..1427
                     /locus_tag="VW191_000663"
     tRNA            1340..1427
                     /locus_tag="VW191_000663"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1374..1376,aa:Ser,seq:gct)
     gene            complement(1561..1998)
                     /locus_tag="VW191_000664"
     CDS             complement(1561..1998)
                     /locus_tag="VW191_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9623462.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKKEPEKFFELIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEA
                     TNNLIKLIKCNAFGFRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(2205..3140)
                     /locus_tag="VW191_000665"
     CDS             complement(2205..3140)
                     /locus_tag="VW191_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEU9623463.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKA
                     KAASLFKATAPITRNELIIEIWRAFFETPSEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(3137..3997)
                     /locus_tag="VW191_000666"
     CDS             complement(3137..3997)
                     /locus_tag="VW191_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEU9623464.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVNLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            4105..6267
                     /locus_tag="VW191_000667"
     CDS             4105..6267
                     /locus_tag="VW191_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9623465.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            6359..7618
                     /locus_tag="VW191_000668"
     CDS             6359..7618
                     /locus_tag="VW191_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9623466.1"
                     /translation="MEWYKKIGLLVTTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            7632..8498
                     /locus_tag="VW191_000669"
     CDS             7632..8498
                     /locus_tag="VW191_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9623467.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            8512..9345
                     /locus_tag="VW191_000670"
     CDS             8512..9345
                     /locus_tag="VW191_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9623468.1"
                     /translation="MKQDERKALIGKYILLVLGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            9346..9426
                     /locus_tag="VW191_000671"
                     /pseudo
     CDS             9346..9426
                     /locus_tag="VW191_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            9703..11145
                     /gene="gtfA"
                     /locus_tag="VW191_000672"
     CDS             9703..11145
                     /gene="gtfA"
                     /locus_tag="VW191_000672"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEU9623469.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            11283..12476
                     /locus_tag="VW191_000673"
     CDS             11283..12476
                     /locus_tag="VW191_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEU9623470.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            13163..15142
                     /locus_tag="VW191_000674"
     CDS             13163..15142
                     /locus_tag="VW191_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623471.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            15209..16705
                     /locus_tag="VW191_000675"
     CDS             15209..16705
                     /locus_tag="VW191_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623472.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16705..17631
                     /locus_tag="VW191_000676"
     CDS             16705..17631
                     /locus_tag="VW191_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623473.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17640..18707
                     /locus_tag="VW191_000677"
     CDS             17640..18707
                     /locus_tag="VW191_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623474.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRSQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18718..19644
                     /locus_tag="VW191_000678"
     CDS             18718..19644
                     /locus_tag="VW191_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623475.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(19719..21053)
                     /locus_tag="VW191_000679"
                     /pseudo
     CDS             complement(19719..21053)
                     /locus_tag="VW191_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000530077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(21208..21918)
                     /gene="treR"
                     /locus_tag="VW191_000680"
     CDS             complement(21208..21918)
                     /gene="treR"
                     /locus_tag="VW191_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEU9623476.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKEPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            22103..24070
                     /gene="treP"
                     /locus_tag="VW191_000681"
     CDS             22103..24070
                     /gene="treP"
                     /locus_tag="VW191_000681"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEU9623477.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            24248..25873
                     /gene="treC"
                     /locus_tag="VW191_000682"
     CDS             24248..25873
                     /gene="treC"
                     /locus_tag="VW191_000682"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEU9623478.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            26129..26377
                     /locus_tag="VW191_000683"
     CDS             26129..26377
                     /locus_tag="VW191_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEU9623479.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26584..27378
                     /gene="racE"
                     /locus_tag="VW191_000684"
     CDS             26584..27378
                     /gene="racE"
                     /locus_tag="VW191_000684"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU9623480.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27375..28346
                     /locus_tag="VW191_000685"
     CDS             27375..28346
                     /locus_tag="VW191_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEU9623481.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            28322..28843
                     /locus_tag="VW191_000686"
     CDS             28322..28843
                     /locus_tag="VW191_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU9623482.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28840..29301
                     /locus_tag="VW191_000687"
     CDS             28840..29301
                     /locus_tag="VW191_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9623483.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            29292..30032
                     /gene="xerD"
                     /locus_tag="VW191_000688"
     CDS             29292..30032
                     /gene="xerD"
                     /locus_tag="VW191_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU9623484.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            30032..30760
                     /locus_tag="VW191_000689"
     CDS             30032..30760
                     /locus_tag="VW191_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEU9623485.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30779..31348
                     /locus_tag="VW191_000690"
     CDS             30779..31348
                     /locus_tag="VW191_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEU9623486.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31335..32057
                     /locus_tag="VW191_000691"
     CDS             31335..32057
                     /locus_tag="VW191_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEU9623487.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            32057..32299
                     /gene="yidD"
                     /locus_tag="VW191_000692"
     CDS             32057..32299
                     /gene="yidD"
                     /locus_tag="VW191_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU9623488.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(32524..33489)
                     /locus_tag="VW191_000693"
     CDS             complement(32524..33489)
                     /locus_tag="VW191_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623489.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33585..34337)
                     /locus_tag="VW191_000694"
     CDS             complement(33585..34337)
                     /locus_tag="VW191_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623490.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(34334..35290)
                     /locus_tag="VW191_000695"
     CDS             complement(34334..35290)
                     /locus_tag="VW191_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEU9623491.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(35280..36239)
                     /locus_tag="VW191_000696"
     CDS             complement(35280..36239)
                     /locus_tag="VW191_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623492.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36662..37363)
                     /gene="galE"
                     /locus_tag="VW191_000697"
     CDS             complement(36662..37363)
                     /gene="galE"
                     /locus_tag="VW191_000697"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9623493.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVG
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT        10668 a         7054 c         8315 g        11383 t
ORIGIN      
        1 aggtcccgta gtgtagcggt tatcacgtcg ccctgtcacg gcgaagatcg cgggttcgat
       61 tcccgtcggg accgtttaag gtaacgcaag ttattttaga ctcgttagct cagttggtag
      121 agcaattgac ttttaatcaa tgggtcactg gttcgagccc agtacgggtc atatatgcgg
      181 gtttggcgga attggcagac gcaccagatt taggatctgg cgcttaacgg cgtgggggtt
      241 caagtccctt aacccgcatt aagatataac aaatgagccg gcttagctca gttggtagag
      301 catctgattt gtaatcagag ggtcgcgtgt tcaagtcatg tagccggcat ttttagatag
      361 aagaaaacag tgcgaacgta gttcagtggt agaacaccac cttgccaagg tgggggtcgc
      421 gggttcgaat cccgtcgttc gcttagagag gccggggtgg cggaactggc agacgcacag
      481 gacttaaaat cctgcgattg gtaacgatcg taccggttcg attccggtcc tcggcataat
      541 atggagcacc cttagctcaa ctggatagag tacctgacta cgaatcaggc ggttagaggt
      601 tcgactcctc tagggtgcat ttttctattt aactcgggaa gtagctcagc ttggtagagt
      661 acttggtttg ggaccaaggt gtcgcaggtt cgaatcctgt cttcccgatt catggcggtg
      721 tagctcagct ggctagagcg tccggttcat acccgggagg tcgggggttc gatccccttc
      781 gccgctataa tgatcttgtc ggacctttag ctcagctggt tagagctctc ggctcataac
      841 cgagtggtcg taggttcaag tcctacaagg tccatttgaa tattggagga ttacccaagt
      901 ccggctgaag ggaacggtct tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa
      961 tcccacatcc tccttttata ttaacgcggg atggagcagc tcggtagctc gtcgggctca
     1021 taacccgaag gtcgtaggtt caaatcctgc tcccgcaata aggctcggta gctcagttgg
     1081 tagagcaatg gattgaagct ccatgtgtcg gcggttcgat tccgtctcgc gccattttat
     1141 tgatagtatt tatgcgggtg tagtttagtg gtaaaactac agccttccaa gctgttgtcg
     1201 cgagttcgat tctcgtcacc cgctttgaac tttgttcaaa ttaccaagtt ttttaacttg
     1261 ggcgcgtagc tcaggtggtt agagcgcacg cctgataagc gtgaggtcgg tggttcgagt
     1321 ccactcgtgc ccattaattg gagaattact caagaggctg aagaggacgg tttgctaaat
     1381 cgttaggtcg ggtaactggc gcaagggttc gaatccctta ttctccgttt taatgagagt
     1441 ttgtaactct cttttttatt tttagagaca ataaagcttc agatccgtaa aacttaattg
     1501 aattactaat ctgaagttat ttttatggct ctttgtcaac tgtagtgggt tgaagaaaag
     1561 ctaagctcga gaaaggacaa atttcgtcct ttcttttttg atgttcaaag cgataaaaat
     1621 ccgtttttta aagttttcaa agtttcgaaa accaaaggca ttgcacttga taagtttgat
     1681 gagattattg gtcgcttcca gtttggcgtt agaatagtgt agttgaaggg cgttgacaat
     1741 cttttcttta tctttgagga aggttttaaa gacagtctga aaaataggat gaacctgctt
     1801 aagattgtcc tcgataagtt cgaaaaattt ctctggttcc tttttcttca ttagacttcc
     1861 acaatcaggg caagatggag cctcataatc cagcttagcg ataatttctt tgtgggtatc
     1921 catattgatg atatctagaa tcttgatgtt tgggtcttta atatcgagca gttttgtgat
     1981 aaaatgtaat tgttccatat gaatctttct aatgatggtt ttgtcgcttt tcattatagg
     2041 tcatatggga ctttttttct acaacaaaat aggctccata atatctataa gggatttacc
     2101 cactacaaat attatagagc caataaaact tcagatccgt aaaacttaat tgaattacta
     2161 atctgaagtt atttttatat cgtttatatt ataattgtgt tattttactt ccaactattg
     2221 agagaaattt cgccactatt tagccagatt tcttttccgt tatcacattg aactaaaagt
     2281 tttccattct ctgagatgtc tttagcaagt cccttgtagt ctttttgctc tagtgtgaaa
     2341 gtgacttctt ttcctagaat gaatgactgt tttttgtata ggtataatag ctcttctgat
     2401 ggtgtttcga agaaagcacg ccagatttct atgatcaatt cattccttgt tataggagct
     2461 gtagctttaa ataagctggc agcttttgct tttaattcct gagggaagtc tttaatagtg
     2521 aaattgatac ctactccaat aatgatatct gtgactaagc cagtttctac agaggtcatt
     2581 gcttcagtaa ggattcctcc aattttatga ttgtttagat agatatcatt gacccatttt
     2641 atgtcgacat ctattaaagt taggttctta atggctttgt agacagctcc agctacaagt
     2701 agtgtgtagg atggtaattt gtcatagggg agatttggtt taagatggag tgtcatataa
     2761 ataccacctt gtggtgagta gaaggaacgt tgaaaacggc ctcggcctgc tgtttgatag
     2821 gaagctagat agagggtatt tgcttcatgg cctaaatcaa ttgcttcttt tgcatctagt
     2881 tgtgttgatt ttgtttcggg tttaaagctg actttaattg gaagattttc ttctagaatc
     2941 tctggaagaa taaggtcacc attcattagt ttatatcctc tatttttgat actatcaatt
     3001 tcaatgcctt cttgttctag tcgcttgata gctttccaaa ttgatgttcg gcttagggat
     3061 agtttttctg cgattttttc tccgctgata tagtcggttt ctttagatag gatttggtag
     3121 acagcttggt aggatttcat aatgttgcct ttctcactag ttggtattga gagtattctt
     3181 ttcttgtatg acttggagtc tgattaaagt attgtttata agctttcgaa aaatggagtg
     3241 gatctgaaaa acctaccgag tatgcaatta ccttgatgga ctcttgggta ttttcgagaa
     3301 gttgtctagc tcggtgcatt cgaacgtaga gtaggtattc tttgggtgat aaggtattaa
     3361 attctttgaa tacgcttgat aagtagcttc tgtgaacgga tagttctttt gctaaatctt
     3421 gaattgtaag tgattgagga tagtggctat caattaatcg tttgcattca agatagagtt
     3481 ggtgggttga tgaaatattc ttttttttct gattgggagc aatagttccc agatgaaaca
     3541 tcagttcatg aagttgtccc atgatatgga gttgagctaa ttcacttgat tttgtaatct
     3601 gagcgaagcg gacaatgtct gagatgagtt ttgcagtagt ctgggtatga caagtttcag
     3661 attggatgag ataggattga tcagaaattt gagaaagagc aaaataatca ggggatttcc
     3721 ctccagtgat tcctaaccag tagtaggccc aaggttcttt actatctgct tgataaaagg
     3781 ttagttcctc tggttttaat agaaagaaat ctccttcttt taaattaaca attttaccct
     3841 tgtaatgaaa ttttccttgt cctttagtaa tgtaatgtag gacgtatgta tcacgaatgg
     3901 ctggaccaaa agagtaatta ggtgtgcatt cctcatatcc ataaaagctt agggcaaggt
     3961 cgattgttcc agtctggtat tctgaaaaaa ctaacatgtg ctacctccta cctaacattt
     4021 taccatattc ttgagacatt tttctatttt agaaagcgat ttcaggtgat aaaatataat
     4081 caataaagtg atgaggtgaa gtaaatggga gttaggatag agaataatct attttatgtt
     4141 gagagtaaaa atctaagttt gattattgaa aatcgaaatg gctacttact tttgaaacat
     4201 ttaggaaaga ctattaagaa ctataaaggt tccaatagtg tttatgaacg agaccatgcc
     4261 ttttcaggaa atccaacggc tactaatcga acctttagtt tagatactca gcgacagatt
     4321 tttggacaac atggcttagg agattttagg aaaccaacca tacaggttca gcatagtgta
     4381 actgaagtaa cagactttcg atttgtagaa gcaaagattt taaaaggtca gaatggtcca
     4441 cagggcttac cttctccaca tagcatggac gatacagaga ctcttgtctt aatgttagaa
     4501 gattctaagg ctcaacttag tctgactttg tattatacta cttttaataa tgatgcgact
     4561 attgctagct acagtaaatt agataataat agtaatcagg aagttgtcat ccataaagat
     4621 ttttctttta tggctgattt tccagctgca gattacgaaa tagtaactct gcagggtgct
     4681 tatgctcgtg aaaagactgt tagacgtcaa caggtagaac aaggaatctt ttcgattagc
     4741 tcaaaccgag gtgcttctgg tcatgctcaa acaccagctc ttctactatg cgaacaagga
     4801 gtcacagagg atgctgggaa tgtgtttgct attcaactaa tgtatagtgg caactttgaa
     4861 gcttttgttc aaaaaaatca attgaatgaa gttcgggtgg ctattggcat taatccagaa
     4921 aacttttctt ggaagttagc ccctgaggaa tactttgaaa caccggtagc tttagtgact
     4981 cattcagatc agggattaac tggtattagt catgaaagtc agaattttgt actgaagcac
     5041 attattctaa gtgaattttc taaaaaagaa cgtccaattc taatcaataa ctgggaagct
     5101 acttactttg actttcagag agaaaaactg ttagagttag cagatgaagc taagaaagtt
     5161 ggcattgaac tttttgtatt agatgatggt tggtttggca atcgttttga tgataatcgt
     5221 gctttaggtg attgggttgt taatgaggaa aaactgggtg gaagtctaga aagtctgatt
     5281 tcagctatcc atgaaagagg tttgcagttt ggactttggt tagaacccga aatgatttct
     5341 gtagatagtg atttgtatcg tcaacatcct gactgggcta ttcaggttcc tgattatgag
     5401 catacttatt ctcggaatca attagtactt aatcttgcca atcctcaggt agtagaatac
     5461 ttgaaaagtg tcttagatca actcctatct tatcatgaga ttgattacat taaatgggat
     5521 atgaaccgca atatcactaa gctagggaat ggattaactt atctagagac acagatgcaa
     5581 tctcatcagt acatgctggg gctttacgaa ctcgtttctt atctgacaga gaagcacagc
     5641 catattctct ttgagtcctg ctctggtggt ggtggacgaa atgatcttgg tatgatacgc
     5701 tatttcccac aagtctgggc tagtgataat actgatgcca ttgcacgttt accaattcaa
     5761 tacggttcat cctatctcta tccaaccatt tctatggggg ctcatgtgtc agcagtaccg
     5821 aatcatcaga tgggacgaat gacaccatta gaaacacgtg gccatgtagc aatgatggga
     5881 aatttgggct atgagcttga tttgacaaat ttatcagatg aagagaaagc tacgattgct
     5941 aatcaggtga acttgtataa agaattacga ccagtagttc agttaggaca gcagtataga
     6001 ctaattaatc ctgatactgc atccaatgaa gcagctgtac aatttaatta cggaaatcaa
     6061 acgattgtaa cctacgttcg cgttttatct gttgtagaga ccatggaaac aactttaaaa
     6121 ttaaaagatt tggatgaaga gggactatat aaattacagg aaaatggcga agtttactca
     6181 ggtgcagaac taatgtatgc gggcttaact gttatcttat cccaaggaga ttttttgagt
     6241 agacagtata tttttagaaa actatgatat gtactgaccc caaaagttag atttattctg
     6301 tctaactttt ggggtgcagt tcaataaggg tgtataaatt tataaaggag gctttctaat
     6361 ggaatggtat aaaaaaatcg gacttcttgt aactacaggt ttagctttgg ttgggctcgg
     6421 cgcttgctcc aactatggta aatctgcgga tggcacagtg accatcgagt atttcaacca
     6481 gaaaaaagaa atgaccaaaa ccttggaaga aatcactcgt gattttgaga aggaaaaccc
     6541 taagatcaag gtcaaagtcg tcaatgtacc aaatgctggt gaagtattga agacacgcgt
     6601 tctcgcagga gatgtgcctg atgtggtcaa tatttaccca cagtccatcg aactgcaaga
     6661 atgggcaaaa gcaggtgtct ttgaagattt gagcaacaaa gactacctga aacgcgtgaa
     6721 aaatggctac gctgaaaaat atgctgtaaa cgaaaaagtt tacaacgttc cttttacagc
     6781 taatgcttat ggaatttact acaacaaaga taaattcgaa gaactgggct tgaaggttcc
     6841 tgaaacctgg gatgaatttg aacagttagt caaagatatc gttgctaaag gacaaacacc
     6901 atttggaatt gcaggtgcag atgcttggac actcaatggt tacaatcaat tagcctttgc
     6961 gacagcaaca ggtggaggaa aagaagcaaa tcaatacctt cgttattctc aaccaaatgc
     7021 cattaaattg tcggatccga ttatgaaaga tgatatcaag gtcatggaca tccttcgcat
     7081 caatggctct aagcaaaaga actgggaagg tgctggctat accgatgtta tcggagcctt
     7141 cgcacgtggg gatgtcctca tgacaccaaa tgggtcttgg gcgatcacag cgattaatga
     7201 acaaaaaccg aactttaaga ttgggacctt catgattcca ggaaaagaaa aaggacaaag
     7261 cttaaccgtt ggtgcgggag acttggcatg gtctatctca gccactacca aacatccaaa
     7321 agaagccaat gcctttgtgg aatatatgac ccgtccagaa gtcatgcaaa aatactacga
     7381 tgtggacgga tctccaacag cgatcgaagg ggtcaaacaa gcaggagaag attcaccgct
     7441 tgctggtatg accgaatatg cctttacgga tcgtcacttg gtctggttgc aacaatactg
     7501 gaccagtgaa gcagacttcc ataccttgac catgaactat gtcttgaccg gtgataaaca
     7561 aggcatggtc aatgatttga atgccttctt taacccgatg aaagcggatg tggattagga
     7621 gtagagagac tatgaaaaaa gtattacaaa aatattgggc atgggctttt gtggtcatcc
     7681 ccctcttgtt acaagcaatt ttcttctatg tgccgatgtt tcaaggagcc ttttacagtt
     7741 ttaccaactg gacaggattg acttataact acaaatttgt tggcttaaac aactttaagc
     7801 tcctcttcat ggatccaaaa ttcatgaatg cgattggctt taccgcaatc attacgattg
     7861 ccatggtggt tggtgagatt gcactcggga tcttcattgc gcgtgtcttg aattctaaaa
     7921 tcaaaggcca aaccttcttc cgtgcttggt tcttcttccc agctgtttta tctggtttga
     7981 cagtggcttt gatcttcaag caagtcttca actacggtct tccagcgatt gggaatgccc
     8041 ttcatattga atttctccaa accagtcttt tagggactaa gtggggagca atctttgcgg
     8101 ctgcctttgt ccttctttgg caaggggtgg ctatgcccat catcatcttc ctagctggtt
     8161 tgcaatctat tccaactgag attacagagg cagcaaggat tgatggtgcg actagcaagc
     8221 aagttttctg gaacattgaa ttgccttact tgctaccaag tgtctctatg gtctttatcc
     8281 tagccctaaa aggtgggctg actgcctttg accaagtctt tgccatgacc ggtggtggtc
     8341 caaacaatgc cacaacctca cttgggctct tggtttataa ctatgccttt aaaaacaacc
     8401 aattcggtta tgccaatgcc attgccgtaa tcttgttcct cttaattgta gtgatttcga
     8461 tcatccaatt gagagtatct aagaaatttg aaatttaaga ggagaagcat gatgaaacaa
     8521 gatgaaagaa aagccctgat tggcaaatac attctattgg ttctaggatc ggttctgatt
     8581 ttagtgccgc tccttgctac cctctttagt tccttcaaac ccactaagga tattgtagat
     8641 aatttctttg gctttccaac caacttcaca tgggacaact ttagccgtct cttagctgat
     8701 gggattggag gctattattg gaactctgtc gtcatcactg tcttgtcttt acttgcagta
     8761 atgatcttta tccctatggc agcctactcc atcgctcgca atatgagtaa aagaaaagcc
     8821 tttaccatca tgtataccct cttaatcctc ggaatattcg tacctttcca agtcatcatg
     8881 attccgatta cggttatgat gagtaaactc ggtttggcta atacctttgg tttgatcttg
     8941 ctctacttga cctatgcgat tccacagacc ctctttctct atgttggcta tatcaaaatc
     9001 tcgattccag aaagtctgga tgaagcagca gagatcgatg gggctaatca atttacaacc
     9061 tatttccgca tcatcttccc aatgatgaaa ccgatgcatg cgacaaccat gatcatcaat
     9121 gccctttggt tctggaatga cttcatgttg ccactccttg tattgaaccg ggattccaaa
     9181 atgtggactc tgcctttgtt ccaatacaac tacgcaggcc aatatttcaa cgactacgga
     9241 ccaagctttg cctcttacgt ggtcggcatt atcagtatca ccattgtcta tctcttcttc
     9301 caacgccata tcatcgcagg aataagcaac ggggcagtga agtaatactc aatgaaaatc
     9361 aaagagcaaa ctaggaagct agccgcaggt tgctcaaaac actgttttga ggttgtggat
     9421 gaaactgacg aagtcagtaa ccatacctac ggtaaggcga cgctgacgtg gtttgaagag
     9481 attttcgaat agtataagaa gagctcatgg aatctatagt ctttgacaca gaggaagagt
     9541 tcagcttgtt gataattcta tgtagaaaac aagaaagtag tactttgttt atcacagtct
     9601 atattagtac gagtttgatg ataaatagag atttaaaatg aatttgattt acgaagccag
     9661 atcatatgag acttggcttc aattagaaaa aggagagtaa tgatgccaat tcaaaataaa
     9721 accatgttga ttacctattc tgatagcctt ggaaataatc ttaaagactt atatgataat
     9781 ttggaagagc attttggaga tgctattgga ggagttcacc ttttaccatt tttcccatca
     9841 acaggtgatc gtggatttgc gccagttgac tacgacgaag tggactcagc ttttggtgat
     9901 tgggaggatg ttaagcgttt aggtgagaga tattatctta tgtttgattt tatgattaat
     9961 catatttctc gtcaatccaa gtattataag gactatcaag aaaaacatga agccagtgaa
    10021 tttaaagatc tctttttaaa ctgggataag ttttggccag aaaaccgtcc gacacagtct
    10081 gatgtagatt taatttacaa gcgtaaggat cgtgcaccaa agcaagagat tgtgtttgaa
    10141 gatggttcag tggaacattt gtggaatacc tttggtgagg agcagattga tcttgatgtg
    10201 accaaagaag taactatgga atttatccgt aagaccattc agcacttggc aagtaatggg
    10261 tgtgatttga ttcgtctaga tgcctttgct tatgcagtga agaaattgga tactaatgat
    10321 ttctttgtgg aaccagatat ttgggattta ttggacaaag ttcgagatat cgctgccgag
    10381 tatgggacag agcttttacc tgagattcat gaacactatt cgattcagtt taaaatagca
    10441 gaccatgatt actatgttta tgattttgct cttccaatgg tgacacttta tactctttac
    10501 agttccagaa cagagcgttt ggctaagtgg ttaaagatga gcccgatgaa gcaatttacg
    10561 acgctagata cccatgatgg gattggagta gtagatgtca aggatatcct gaccgatgag
    10621 gagattgact atgcttcaaa tgaactctat aaggttggag ccaatgtcaa acgtaagtac
    10681 tctagtgccg agtataacaa cttagatatc taccaaatca attcaaccta ctattcagcg
    10741 cttggagatg atgatgtcaa gtattttctc gctcgtctaa ttcaagcttt tgccccaggt
    10801 attcctcagg tttactatgt gggtctatta gcaggcaaga atgacttgaa attattagaa
    10861 gaaactaaag aaggtcgaaa tattaatcgt cattactata gcaacgagga aatagcaaaa
    10921 gaagtgcaac gacctgttgt gaaggccctt ctcaatctat tttctttccg taaccgttca
    10981 gaagcctttg atctagaagg gactactgag atagagacac caacagctca cagcattgta
    11041 atcaaacgtc aaaataaaga taagtccgta acagcagtag tagaaattga tttgcaaaat
    11101 cagacttatc gggtaattga gaatggagtt gaagtatcat tttgaagcct tgatatggtg
    11161 gataaaatag ggtttttatt ttggaaaacg tttcctttgt tttcaaattg ctaaaaaagt
    11221 ggtacaatat aggatagctt actattatct gaatcagctg atttggagag aaaggattca
    11281 ttttgaaatc aataggcttt attgaaaagc tgaaggggtt gtctagtaaa gagctgattt
    11341 tattgggaat tatcctaagt atctttttac ccttttatct ttttgtagtt gtactctgtt
    11401 tatatattat cagtttgatt tttacaggag acatgaaaag tattcttcag aaaatggggg
    11461 agcatccgat gctgcttctt tttcttagct atagtactgt tatatccatt cttgcacaaa
    11521 attggatggg tcttgtggct tcagtaggaa tgtttctatt tactattttc tttttgcact
    11581 atcagtcgat tttatcccat aaattctttc gattgatttt gcagttcgtc ttgtttggta
    11641 gtgtcttgtc agctgctttt gccagtttag aacatttcca aattgtgaag aaatttaact
    11701 atgcttttct ttcacccaat atgcaggtgt ggcatcagaa ccgggcagaa gtgaccttct
    11761 ttaatcctaa ttattatgga attatttgtt gtttctgtat tatgattgct ttctatctgt
    11821 ttacaacgac caagttgaat tggttgaaag tattctgtgt gattgcaggc tttgttaatc
    11881 tctttggttt gaactttact caaaatcgaa ctgcctttcc tgctattatc gctggagcaa
    11941 ttatctatct ctttacgact attaaaaact ggaaggcctt ttggcttagt attggggtct
    12001 tcgcgattgg tttgagtttc ctcttttcta gtgatttggg agttcgaatg ggtactttag
    12061 actcttctat ggaagaacgc atttctatct gggatgctgg gatggccttg tttaagcaaa
    12121 atcctttttg gggtgaaggg ccattgacct atatgcactc ttatcctcgg atacatgctc
    12181 cttatcatga acatgcccac agtctttata ttgatacgat tctgagttac ggaattgtgg
    12241 gtaccatttt attagttttg tcttctgttg ctcctgttcg cttgatgatg gatatgagtc
    12301 aggagtcggg gaaacgtccg attatcggcc tttatctatc tttccttaca gtggttgctg
    12361 tgcacggaat ttttgacttg gctctcttct ggattcagtc aggctttatt ttcttgctag
    12421 ttatgtgcag tattccattg gagcatcgaa tgttggtatc ggacatgacg gattaagttt
    12481 tataagtttg aaatcttcta ccttgggtag gagatttttt tatacgatgg gcatgtcgtg
    12541 catctgaggt gtaagtcctc tgtagggatc cactaccggt gaatacaata gcgctcccca
    12601 agcctgacta tcgtgaggta gcggattaaa atggtctggg gatagatcgt tttaagtctg
    12661 acgcaggaaa taagaattgt cagagaaagc aatagtgaaa tcgtaggcta tgaatagaaa
    12721 cgtgatagcg aggagcatat agtggataag gtagcgagaa attttaaagt ataaaagata
    12781 gtcttaatct atatatgtaa atcacaaaag taattgaatt cggaataagg ttggtgtgaa
    12841 aagacatagt aaactgaatc ttgaatacta gaatgatact tctacaatat tgttataatt
    12901 taatttgaat tccaaaatca cattgttccg tccgtatttc attttactgt atgtcttcct
    12961 aaactccaaa gtttctgcgt caaatttcct attttcactg tagtctttga cacttcgaac
    13021 gacaatttga atcatatatt caaaaaaaaa tgaaaaattt gtgccaacaa gcctaaatat
    13081 tctgaaaatt tgtttgattt ttaatggata atgtgatata atggttgtca atctataatt
    13141 cacataaaca gaggagtaga aaatgaaaaa aaatcgtgta tttgctacag caggtcttgt
    13201 tttattagca gcaggtgtac ttgcagcatg cagttcttca aaatcatctg attcatcagc
    13261 ccctaaagct tatggctatg tttatacagc agacccagaa accttggact acctgatttc
    13321 aagtaaaaat agtacaacag tagtgacttc aaatgggatt gatggtttat tcactaacga
    13381 taattacggt aatcttgctc ctgcagttgc agaggattgg gaagtctcta aggatggttt
    13441 gacctacact tataagattc gtaaaggggt taaatggttt acctctgatg gagaagaata
    13501 tgcagaggtg acggctaaag attttgtgaa cggtttaaaa cacgcagcag ataaaaaatc
    13561 agaagctatg tatttagctg aaaattcggt taaaggcttg gcagattatc tatcaggaac
    13621 ttcaacagat ttttcaacag ttggtgtcaa ggcggttgat gattatacgt tacaatacac
    13681 tttgaaccag cctgaaccgt tctggaactc taagttgacc tattctattt tctggcctct
    13741 gaatgaagaa ttcgaaacat caaaaggaag cgattttgct aaaccaacag atccgacatc
    13801 cttgctttat aatggtccat tcttgttgaa agggttgact gcaaaatctt ctgtagagtt
    13861 tgtaaaaaat gagcaatatt gggataaaga aaatgtccac ctagatacta tcaatctagc
    13921 ttactatgat ggatcagatc aggagtcgct agagcgtaac ttcactagtg gagcttatag
    13981 ttatgcccgt ctttacccta ccagctccaa ctattctaag gttgcagaag aatacaagga
    14041 caatatctat tacacacaat caggctctgg gattgctggt ctgggtgtga atattgatcg
    14101 ccaaagttac aactatactt ctaaaactac agattcagag aaagtagcta ctaagaaggc
    14161 attgcttaac aaagatttcc gtcaagcctt gaattttgct cttgatcgct cagcttactc
    14221 agctcaaatc aatggtaaag atggagcagc tttagcagtt cgtaatttat ttgtaaaacc
    14281 agactttgtt tcagctggtg agaagacctt tggtgattta gtcgctgctc aacttcctgc
    14341 ttatggtgat gagtggaaag gtgtgaattt agctgatggg caggatggtt tattcaatgc
    14401 tgacaaggcc aaggcagagt ttgcgaaagc taagaaagct ttagaagcag acggcgttca
    14461 gtttcctatt catctggacg ttccagtaga ccaagcatca aaaaactaca tatctcgtat
    14521 tcagtccttt aaacaatctg tagaaacagt tcttggtgtt gaaaatgtcg ttgttgatat
    14581 tcaacaaatg acaagtgatg aattccttaa tattacttac tatgctgcca atgcttcatc
    14641 tgaggattgg gatgtatcgg gaggagtttc atgggggcca gactatcaag acccatctac
    14701 ttacctggat attttaaaaa caactagcag tgaaactaca aaaacatatt taggatttga
    14761 taatccaaat agcccttcag tagttcaagt tggtttgaaa gaatacgata aattagttga
    14821 tgaagctgcc aaagagacaa gcgacttgaa tgtccgttat gaaaaatatg cagcggctca
    14881 agcatggttg acagatagtt cactctttat tcctgctatg gcttcttctg gtgcagcacc
    14941 agtgctttca cgaattgttc catttactgg agcttctgcg caaacaggct ctaaggggtc
    15001 agatgtttac ttcaaatatt tgaaattaca agataaagtg gtgactaagg aagagtatga
    15061 aaaagctcgt gaaaaatggt tgaaagaaaa agctgaatca aatgagaaag ctcaaaaaga
    15121 attggcaagt catgtgaagt aaataatttc taatagaact ttctctccat gaggagaagg
    15181 ttcttttggg atttttaaag gaaataatat gaaaaaatat atttttatgc gtgttttgcg
    15241 gtcattggtt tcgattttct tagtaacgac tttgacctac acgattatct ataccttggt
    15301 tcctcgaaaa ttgattttca agcaggatcc taactataat aaaattgcga caacggctga
    15361 taaacgtgat aactatgaaa atactgtgtt tgagcgtatg ggctacattg agtattacga
    15421 tactaaagag ttgcaagaaa aggcaagtag catggattct tctgtaacag tagaagcaaa
    15481 tgcgaccaat aaagctattt atgaaaagta catcaatcaa ttaggtcatg gttggacttt
    15541 gggagaattt actgaaagtg gtcaattcta tgctactcgt gaaattccaa tttttgaacg
    15601 tgtttttcac ttctatgcta acttgattga cattgaccat acaaataaaa tccaagaccc
    15661 tgaaaatcca gacttgaaac gctaccttcg ttttgaaaat gatccagcta tcggatggtc
    15721 attggtcggt tcaggaacta aacataaata tctcttgtac tttaacagtc agttcccatt
    15781 tgttcatcaa aactttgtga acttgaattt aggtgactct tacccaacct atgctaatac
    15841 accagttctt caggttatta ctcaaggtca aggacaaacc aaaactgccc aagttcagtt
    15901 cccaacaggt aagaaaacgt cttctgtaaa tatttactca agaacctaca agtcacctag
    15961 tcaggctgac tctcgtgaag tagctagcta tgggaaagat gatccttata cagcgactga
    16021 aagtaattac caatatccat ctatgattgt cagctctgct attactggtt tgattggttt
    16081 ggttcttgcc tatgctcttg ccgtgccact tggttcagcc atggctcgtt tcaagaacac
    16141 ttggattgat agcctctcaa cagggacttt gaccttcttg cttgctcttc caacgattgc
    16201 cttggtttac atcgttcgat tgattggatc atctattgcc cttccagatt cattccctat
    16261 cttgggagct ggagattggc gttcttacgt tttaccagca gtcatccttg gtttgttggg
    16321 tgctcctgtt acagccattt ggattcgtcg ttacatgatt gacttgcaat ctcaagactt
    16381 tgttcgtttc gctcgtgcaa aaggtttgtc tgaaaaagaa atttcaaaca aacacatctt
    16441 taaaaatgcc atggttccgc tggtttcagg aattcctggt gccattattg gggttatcgg
    16501 tggtgcaacc cttactgaaa cagtcttcgc cttcccaggt atgggtaaaa tgttgattga
    16561 ctctgtaaaa gcatctaata actctatggt cgttggtctt gtcttcatct ttacatgtat
    16621 ttctatcttc tcacgtcttt tgggagatat ttggatgact attattgacc cacgtattaa
    16681 attgactgag aaaggaggca aataatgtct acaatcgata aagaaaaatt tcagtttgta
    16741 aaacgtgacg attttgcctc tgaaactatt gatgcgccag catattctta ctggaaatca
    16801 gtgtttaaac aatttatgaa gaaaaaatca actgtagtca tgttgggaat cttggtagcc
    16861 atcattttga taagtttcat ctacccaatg ttttctaagt ttgatttcaa tgatgtcagc
    16921 aaggtaaacg actttagtgt tcgttatatc aagccaaatg cggagcattg gttcggtact
    16981 gacagtaacg gtaaatcgct ctttgacggt gtctggttcg gagctcgtaa ctccatcctc
    17041 atttctgtga ttgcgacagt gattaacttg gttatcggtg tttttgtcgg tggtatttgg
    17101 ggtatttcaa aatcagttga ccgtgtcatg atggaagttt acaacgtcat ctcaaacatc
    17161 ccacctcttt tgattgttat tgtcttgact tactcaatcg gagctggatt ctggaatctg
    17221 atttttgcca tgagcgtaac aacatggatt ggtattgcct tcatgatccg tgtgcaaatc
    17281 ttgcgctatc gtgacttgga atacaacttg gcgtcacgta ctttgggaac accaaccttg
    17341 aagattgttg ccaaaaatat catgcctcaa ttggtatctg ttattgtgac aaccatgact
    17401 caaatgcttc caagctttat ctcatacgaa gccttcttgt ctttcttcgg tcttggatta
    17461 ccgattacag tgccaagttt gggtcgtttg atttcggatt attcacaaaa cgtaacaacc
    17521 aatgcttact tgttctggat tccattgaca acccttgtct tggtatcctt gtcccttttc
    17581 gtagttggtc aaaacttagc ggatgctagt gatccacgta cacatagata ggagtagaaa
    17641 tgacaaaaga aaaaaatgta attttgactg ctcgcgatat tgtcgtggaa tttgacgttc
    17701 gtgacaaagt attgacagcc attcgcggcg tttcccttga actagtcgaa ggagaagtat
    17761 tagccttggt aggtgagtca ggatcaggta aatctgtttt gacaaagacc ttcacaggta
    17821 tgctcgaaga aaatggtcgc attgcccaag gtagtattga ctaccgtagt caggacttga
    17881 cagctttatc ttctcacaag gattgggaac aaattcgtgg tgctaagatt gcgactatct
    17941 tccaggaccc aatgactagt ttggacccca ttaaaacaat tggtagtcag attacagaag
    18001 ttattgtaaa acaccaagga aaaacagcta aagaagcgaa agaattggcc attgactaca
    18061 tgaataaggt tggcattcca gacgcagata gacgttttaa tgaataccca ttccaatatt
    18121 ctggaggaat gcgtcaacgt atcgttattg ctattgccct tgcctgccga cctgatgtct
    18181 tgatctgtga tgagccaaca actgccttgg atgtaactat tcaagctcag attattgatt
    18241 tgctaaaatc tttacaaaac gagtatcatt tcacaacaat ctttattacc cacgaccttg
    18301 gtgtggtggc aagtattgcg gataaggtag cggttatgta tgcaggagaa atcgttgagt
    18361 atggaacggt tgaggaagtc ttctatgacc ctcgccatcc atatacatgg agtctcttgt
    18421 ctagcttgcc tcagcttgct gatgataaag gggatcttta ctcaatccca ggaacacctc
    18481 cgtcacttta tactgacctg aaaggggatg cttttgcctt gcgttctgac tacgcaatgc
    18541 agattgactt cgaacaaaaa gctcctcaat tctcagtatc agagacacat tgggctaaaa
    18601 cttggcttct tcatgaggat gctccaaaag tagaaaaacc agctgtgatt gcaaatctcc
    18661 atgataagat ccgtgaaaaa atgggatttg cccatctggc tgactaggag gaaggaaatg
    18721 tctgaaaaat tagtagaaat caaagattta gaaatttcct tcggtgaagg aagtaagaag
    18781 tttgtcgcgg ttaaaaatgc taacttcttt atcaacaagg gagaaacttt ctcgcttgta
    18841 ggtgagtccg gtagtgggaa aacaactatt ggtcgtgcta tcatcggtct aaatgataca
    18901 agtaatggag atatcatttt tgatggtcaa aagattaatg gtaagaaatc gcgtgaacaa
    18961 gctgcggaat tgattcgtcg aatccagatg attttccaag accctgccgc aagtttgaat
    19021 gaacgtgcga ctgttgatta tattatttct gaaggtcttt acaatcaccg tttatttaag
    19081 gatgaagaag aacgtaaaga gaaagttcaa aatattatcc gtgaagtagg tcttcttgct
    19141 gagcacttga ctcgttaccc tcatgaattc tcaggcggtc aacgtcaacg tatcggtatt
    19201 gcccgtgcct tggtcatgca accagacttt gttattgcag atgagccaat ttcagccttg
    19261 gacgtttctg tacgtgccca agtcttgaac ttgctcaaaa aattccaaaa agagctcggc
    19321 ctgacctatc tcttcatcgc ccatgacttg tcggttgttc gctttatttc agatcgtatc
    19381 gcagttattt acaagggtgt tattgtagag gttgcagaaa cagaagaatt gtttaacaat
    19441 ccaattcacc catatactca agccttgctt tcagcggtac caatcccaga tccaatcttg
    19501 gaacgtaaga aggtcttgaa ggtttacgac ccaagtcaac acgactatga gactgataag
    19561 ccatctatgg tagaaatccg tccaggtcac tatgtttggg cgaaccaagc cgaattggca
    19621 cgttatcaaa aaggactaaa ctagtaatgg ttttataatt tccatgtcaa tttttatgga
    19681 aattaggctc tttgtcaact gtagtgggtt gaagtcagct aagctcgaga aaggacaaat
    19741 ttcgtccttt cttttttgat gttcaaagcg ataaaaatcc gttttttgaa gttttcaaag
    19801 tttcgaaaac caaaggcatt gcgcttgata agtttgatga gattattggt cgcttccaat
    19861 ttggcgtttg aatagggtag ttgaagggcg ttgacgattt tctctttgtc cttttgtcct
    19921 ttagaaaggt tttaaagaca gtctgaaaaa taggatgaac ctgcttaaga ttgtcctcaa
    19981 taagtccgaa aaatttctct ggttccttat tctggaagtg aaaaagcaag agctgataga
    20041 gttgatagtg gtgtttcaag tcttccgaat aactcaaaag cttgtttaaa atctctttat
    20101 tggttaagtg catacgaaaa gtaggacgat aaaaacgttt atcactcagt ttacggctat
    20161 cctgttgaat gagcttccag tagtgcttga tagccttgtg ttcatgggat tttcgatgaa
    20221 actgattcat gatttggaca cgcacacgac tcatagcacg gctaagatgt tgtacaatgt
    20281 gaaagcgatc aagaacgatt ttagcattcg ggagtgaaac agtctgggag actgtttcag
    20341 cctgagccta gaaatttgaa agcgaagctg tttagccaag tcatagtaag ggctaaacat
    20401 atccatagta atgattttga cgcgacatcg gacggctcta tcatatttaa gaaagtgatc
    20461 tcggatgaca gcttgtgttc taccttcaag aacagtgatg atatcgagct tttcaaaatc
    20521 ttgcgcaata aagctcatct ccatctcccg attgaaacag tcactccccg gactgtttca
    20581 acgtcccaag acataatctt aggcagattt ctaaaattac actcaaagtg gaagtcattg
    20641 agctttcgaa tgacagttga agttgagatg gccagctgat gggcaatatc ggtcatagaa
    20701 ttcttttcaa ttaacttttg agcaattttt tggttgatga tacgagggat ttggtgattt
    20761 ttctttacca ggggagtctc agcgaccgct atttttgagc actgatagca cttaaagcga
    20821 cgctttctaa ggaggattct agtaggcata ccagttgtct caaggtaagg aatcttagac
    20881 ggtttttgaa agtcatattt cttcatttga cttccgcact cagggcaaga tggggcgtcg
    20941 tagtccagtt tggcgatgat ttctttgtgt gaatccctat tgacgacatc tataatttgg
    21001 gtatttgtgt ctttgatatc gagtagtttt gtgataaaat gtaattgtcc catatgaatc
    21061 tttctaatga gttgttttgt cgcttttcat tataggtcat atgggacttt ttttctacac
    21121 aaaataggct ccataatatc catagggggg gtacccacta caaatattat agagccgaaa
    21181 aaggctgaga tctggtgtct cagtctttta tttcttgcgt tttgcaaaat ctacaaatct
    21241 aaatttttct aacttatggc gactttcggt aaactgaaat tggcgtccgt cttggagata
    21301 gacttttgat ttaatcgaaa cgacataagg ttctttgcca atgtccatga gaatcttgtc
    21361 tcggtcgctt gagtggtcaa tggtgatttc cttctgagca taatcaataa ggagtttgag
    21421 gccattttct atataagaat agatagactg ctcagcaatt tggcgagtga gatttggaat
    21481 tagttccata tccagatagt ctgtatccag aacggatacc agatcatcca ccacacgctg
    21541 gcggaccacc ttccaaacca tccgaaactc tgggaaaccg gttatcagtg aggatttttt
    21601 atcaataata atcttgtcca gactgaccac gttggtttta gagcgcagtc caagttcttt
    21661 aactagttct tggtagctgg ttaggttgga tacagggaaa ttgacggttt cttctttgac
    21721 gacttgagaa ccttgccctc ttatcttttt gatcaatcct tcctcttgga ggagagacag
    21781 ggcctttcgg acggtatcac gactcacttg atattgctcc ataaggtcgt gctcgctagg
    21841 aaggaaatct ccgacagcgt aagtctcgtt ttgaatggtt tcttggattt gcttaaataa
    21901 ttgttggtat ttcttcattt tgcctgctta aataggtttt tagtctagtt taatgaacac
    21961 ctgtatctat tttaccatac aagtgctaga aaatttacaa aaaaacaccc tttttatacc
    22021 aaacaagttg cagacaagtt tggtatcgtt tacaatatac ttatcaaatc aaacttgctt
    22081 tgacaagtat aaggagaatc aaatgggaaa atttgaacaa gaagccaaag atctgcttca
    22141 ggcaattgga ggcaaagaaa atgtgactgc cgtcacccac tgtgcgacac ggatgcgctt
    22201 tgttttagga gatgataaga aggctaatgt taaagctatc gagtcaattc cagctgttaa
    22261 aggaactttt acaaatgcag gtcaatttca ggtaatcatt ggaaatgacg tgcccatctt
    22321 ttataatgat tttacagccg tttcaggtat tgagggtgtt tccaaagaag cagccaagtc
    22381 tgcagctaag agtaatcaaa acgtggtcca aggtgttatg accactctgg cggagatttt
    22441 tactccgatt attccagcct tgatagtcgg aggattgatc ctcggtttcc gtaatgtctt
    22501 ggaaggtgtc cattggtcga tgttggatgg caagaccatc acagaatcct ctcagttttg
    22561 ggcaggggtt aatcacttcc tctggttgcc tggtgaagct atcttccagt tcttaccagt
    22621 agggattact tggtctgttt ctcgtaagat gggaaccagc caaattttgg gaattgttct
    22681 cggaatctgt ttggtatcgc ctcagttgct caatgcctat gcggttgctt caacgccagc
    22741 agctgatatc gcggcaaact gggtttggaa ttttggttat tttactgtta atcgtattgg
    22801 ttaccaagcc caagttatcc cagccttgct tgcaggtttg agtctgtctt atcttgaaat
    22861 tttctggcgc aaacatatcc cagaagtcat ttccatgatt tttgtacctt ttttgtcatt
    22921 gattccagcc ttgattttgg ctcatactgt tttgggacca atcggttgga caattggaca
    22981 aggactttca tcagttgtct tggcaggttt aactggtcca gttaaatggc tcttcggtgc
    23041 aatttttggt gccctctacg ctccatttgt catcacaggt ctgcaccata tgaccaatgc
    23101 cattgataca caattgattg cggatgctgg tggcactgcc ctatggccaa tgattgctct
    23161 ttctaatatt gctcaaggct cagccgtgtt tgcctattat ttcatgcatc gccatgatga
    23221 gcgtgaggct caggtttcac ttcctgcaac catttcagcc tatctcggtg ttacagaacc
    23281 agctcttttt ggggttaacg taaaatatat ttatccattt gttgctggga tgactggttc
    23341 agcccttgca ggcatgttat ccgttacttt taatgtaact gcggcttcta ttggtatcgg
    23401 tggtttgcca ggtattctct ctattcaacc tcaatacatg ctgccatttg caggaactat
    23461 gctagttgcg attgttgttc caatgctctt gactttcttc tttcgcaagg ctggtctctt
    23521 cacaaaaata gagggcgata cgaacttgca ggcagaattc gttgctcaag aagaagcaga
    23581 atttgtgagc catgaaccag tagaacttac ttcggtagaa attatcagcc cactaactgg
    23641 ccaagtgaaa gaattgagtc aagcgacgga tcctgttttt gcatcaggtg tcatggggca
    23701 aggtctggtc attgaaccaa gccaaggtga gttgacctct ccagttaatg gaacagtgac
    23761 ggttcttttc cctaccaagc atgccatcgg cattgtctct gacgagggag ttgaattgct
    23821 catccacatc ggtatggata cagtaggtct tgatggcaaa ggttttgaaa gtcttgtagt
    23881 ccaaggagat cacgttatag ttggccagca actgattcgt tttgatatgg atgtcattaa
    23941 ggctgcaggt ctggtgacag aaactcctgt tatcatcacc aaccaagatg cttatacagc
    24001 gactattact ggaacttatc cgacaacgat ccaagctgga gcatctctca tggtcgctac
    24061 acgaatctaa tactcttcga aaatctcttc aaaccacgtc agctctatct gcaacctcaa
    24121 aatagtgttt tgagcaacct gtggctagct tcctagtttg ttctttgatt ttcattgagt
    24181 ataattgaaa tagcacaaat agggacggaa gactgcttcc tcccttttat cagaaaagga
    24241 gaaacagatg acacttgata aaggaaaagt agtctatcaa atctatccaa aatcttacaa
    24301 agacaccact gaaaacggta ttggggattt ccgtgggatt atcgaaaaaa ttccctattt
    24361 agccaaacta ggtgtggata tggtctggct caatccattc tatccaagcc ctcaacggga
    24421 taatggttac gatatttcag attatatggc agtggatcct ctttttggtg atatggctga
    24481 ttttgaggaa atggtgtgtg tcggtaaaga gcacaagatt gactttatgc tggacatggt
    24541 acttaatcat tgttcgacag agcatgaatg gtttcagaaa gccctagctg gtgataagta
    24601 ttatcaagac tttttcttca tccaagacca accaacagat tggcagtcta agtttggtgg
    24661 ttctgcatgg gcgcctttcg gggatactgg gaaatactat cttcacctat ttgatgagac
    24721 ccaggctgac cttaactggc gcaatcccaa tgtccgtaag gagcttttca aggttgttaa
    24781 tttctggcgc gacaagggtg tcaaaggttt tcgatttgat gtgatcaatt tgatcggtaa
    24841 ggacgaggtt tcagtggact gtcctgaaaa tgaagggaag ccagcttata cagacaaacc
    24901 catcgttcat aactatttgc gtatgatgaa ccaagccact tttggatctg acgatagctt
    24961 tatgacagtt ggggaaatgt cttctaccac catggaaaac tgtgtccttt attcgtcgcc
    25021 tgatcgtcag gaattatcca tgacctttaa ttttcatcac ctcaaggtgg actataagga
    25081 tggacaaaag tggaccttgg ctccctttga ttttgaagag ttgaagagtc tttaccatag
    25141 ttggggtaag gaaatgagtg ataaagacgg ttggagtgcc ctcttttgga acaatcacga
    25201 tcaaccacgt gccttgaacc gttttgtcga tattcaaaac ttccgcaagg aaggagccac
    25261 catgctagca gccagcattc acctgtcacg tgggacacct tatatctaca tgggtgagga
    25321 aatcgggatg attgacccag actatgattc tatggctgat tatgtggatg tcgaatcgct
    25381 taatgcctat cagatgctct tagaagaagg aaagagtcag caagaagcct tccagattat
    25441 tcaagctaag tcgcgtgata attcacgtat tcccatgcag tgggatactt ctgaaaatgc
    25501 aggattttca acaggcactc catggttgaa agttggtaaa tcctataaag atatcaatgt
    25561 agaaaatgaa atcaaaggtc tgattttcac cttctatcaa gacttgattc gactgcgtaa
    25621 ggaaatgcca atcatttcag aaggaaatta caaaccagcc tttgaagata gcaaacaagt
    25681 ctacgctttt gaacgccagt ttgaggatca aaagttacta gtgctcaata acttttatgc
    25741 caaagaagtg gaaatcgact tgccagcagt ctaccaaaat ggacagattt tgatttcaaa
    25801 ctatgaagat gcagaagtgt ctgagaaaat tctactcaaa ccctatcaaa cactggctat
    25861 ttatgtaaat taatactctt cgaaaatctc ttcaaaccgt gtcagtttta tctgtaacct
    25921 caaagcggtg ctttgagcaa cctgtggcta gcttcctagt ttgctctttg attttcattg
    25981 agtataaact tagcaaggag acctcttttt cagaaaagca gggttctttt tgctgtctat
    26041 tccatccttg aaagtgtaga acaaactggt cagggtggct agtttgtggt ataatgaaag
    26101 ggattcaaaa agaaacagga gaaaaatgat ggatttactt ttagcaattg tattgattgt
    26161 gctagctttt ctaggaggag ctcttggagg aatgtacttg gttcgtaagc aaatcgaaaa
    26221 agaatttgct gacaacccac gtttgaatgc tgaagcagtt cgtactcttt tgagtgcaaa
    26281 tggtcaaaaa ccaagcgaag ctaaggtaca acaagtttac caccaaatca tccgccaaca
    26341 aaaggcagcc cttgctaaca ataaaaagaa aaaataaata aggaaaagtc tgggatgaaa
    26401 gttccagact tctcactatg ttggttatgg tttagggatg agactttttc cttgcattct
    26461 attgatattt gattatgatt aagagagagg tagttgacat tctatcaagc tgtcggtcag
    26521 ttcgttcagt acaaggaatc ataatgaacg atcaatcaga aaaaagacta gaaagaagac
    26581 tgtatggata atcgaccaat tggttttttg gattcgggtg tcgggggctt gaccgttgtg
    26641 cgcgagctca tgcgccagct tccccatgaa gaaatcgtct atattggaga ttcggcgcgg
    26701 gcgccctatg gcccccgtcc tgctgagcaa attcgtgaat atacttggca gctggtcaac
    26761 tttctcttga ccaaggatgt caaaatgatt gtcattgctt gtaacactgc gactgcggtc
    26821 gtctgggaag aaatcaaggc tcaactagat attcctgtct tgggtgtaat tttgccagga
    26881 gcttcggcag ccatcaagtc cagtcaaggt gggaaaatcg gagtgattgg aacgcccatg
    26941 acggtacaat cagacatata ccgtcagaaa atccatgatc tggatcccga cttacaggtg
    27001 gagagcttgg cctgtcccaa gtttgctccc ttggttgagt caggtgccct gtcaaccagt
    27061 gttaccaaga aggtggtcta tgaaaccctg cgtcccttgg ttggaaaggt ggatagcctg
    27121 attttgggct gtactcatta tccactcctt cgccctatta tccaaaatgt gatggggcca
    27181 aaggttcagc tcatcgatag tggggcagag tgcgtacggg atatctcagt cttactcaat
    27241 tattttgaaa tcaatcgtgg tcgcgatgct ggaccactcc atcaccgttt ttacacaaca
    27301 gccagtagcc aaagttttgc acaaattggt gaagaatggc tggaaaaaga gattcatgtg
    27361 gagcatgtag aattatgaca aataaaattt atgaatataa ggatgaccag gactggtatg
    27421 ttgggtctta tagtattttt ggtggcgtta acagtttgag cgactataag acagattttc
    27481 ctctgtttga attctccaaa atatttggag atgaagagta tggtttcccg ctttcagtta
    27541 ctgttttacg ctatggttct acctaccgtt tgttctcctt tgtggtagac atgcttaatc
    27601 aagaaatggg acgaaacttg gaagttattc aacgtcatgg ggccctgctc ttggttgaaa
    27661 atgggcaact cttgtatgta gaattgccta aagaaggggt caatgttcat gatttctttg
    27721 agacaagcaa ggtcagagaa accttgttga ttgcgactcg taacgaaggt aaaaccaagg
    27781 aattccgagc tatctttgat aagttaggct acgatgtgga aaatcttaat gactaccctg
    27841 acctgcctga agtagcagaa acaggtatga cctttgaaga aaatgcccgc cttaaggcag
    27901 aaaccatttc tcaattaacg ggcaagatgg ttttggcaga tgattctggt ctcaaagtcg
    27961 atgtccttgg tggcttacca ggcgtctggt cagctcgttt cgcaggtgtg ggagcaactg
    28021 accgtgaaaa taatgccaaa ctcttgcacg aattggccat ggtctttgaa ctcaaggacc
    28081 gctcggctca gttccacaca accctagtcg tagccagccc aaataaggaa agtttagttg
    28141 ttgaagcaga ctggtcaggt tatattaact ttgaacctaa gggtgaaaat ggctttggct
    28201 atgatcccct cttccttgta ggagagacag gtgagtcatc agctgaatta accctggaag
    28261 aaaaaaatag tcaatctcac cgtgccttag ccgttaagaa acttttggag gtatttccat
    28321 catggcaaag caaaccatca ttgtaatgag cgattcccat ggcgatagct tgattgtgga
    28381 agaagtccgt gatcgctatg tgggcaaagt cgatgccgtt tttcataacg gcgattctga
    28441 actacgtccg gattctccac tttgggaggg catccgcgtt gttaaaggga acatggactt
    28501 ctacgccggc tacccagaac gtctggtgac tgagcttggt tcgaccaaga ttatccaaac
    28561 tcatggtcac ttgtttgaca tcaatttcaa ctttcaaaag ttggactact gggctcagga
    28621 ggaagaggcc gctatctgcc tctatggtca cttgcatgtg ccaagtgctt ggttggaagg
    28681 caagatcctc tttctaaatc caggttctat cagtcaacca cgaggtacca tcagagaatg
    28741 tctctatgct cgtgtggaga ttgatgatag ttacttcaaa gtggactttt tgacacgaga
    28801 tcacgaggtg tatccaggtt tgtccaagga gtttagccga tgattgccaa ggagtttgag
    28861 actttcttgt tggggcagga ggaaactttt ttgacccctg ctaaaaatct agctgtgttg
    28921 attgataccc acaatgcgga tcatgcgacc ctcttgctca gtcagatgac ctatacccgt
    28981 gttcccgttg tgacagatga aaaacagttt gttgggacga ttggactcag agatattatg
    29041 gcttatcaga tggagcatga cttgagccaa gaaatcatgg cggatacgga tatcgttcat
    29101 atgacaaaaa cggacgtagc ggttgtttcg cctgatttca ccattacgga ggtcttgcac
    29161 aagctagtag atgagtcctt cttaccggtt gtggatgcag agggtatttt ccaagggatt
    29221 attacgcgca agtccatcct caaggccgtt aatgccctct tgcatgactt tagtaaggaa
    29281 tatgagattc gatgccaatg agagacagga tttcagcctt tttagaggaa aagcagggct
    29341 tgtctgtcaa ttccaagcag tcctataagt atgatttgga gcaattttta gacatggtag
    29401 gtgagcggat ttctgagacc agtctcaaga tttaccaagc ccagctagcc aatctaaaaa
    29461 tcagcgccca gaagcgaaag atttcggcct gtaaccaatt tctatacttt ctctatcaaa
    29521 aaggagaggt ggacagcttt taccgcttgg aattagccaa acaagctgaa aagaagacgg
    29581 aaaagccaga gattctatac ctagactctt tttggcagga aagcgaccat ccagagggcc
    29641 gcttgctagc gctcttaatc ctagaaatgg ggcttttgcc cagtgagatt ttagccatca
    29701 aggttgcgga catcaatctg gattttcagg tgttgcgaat cagcaaggct tcccaacaga
    29761 ggattgtcac tattcccacg gccttgcttt cagaattgga acccttgatg gggcagacct
    29821 atctttttga aagaggagag aaaccctatt ctcgtcagtg ggcctttcgt cagttagaat
    29881 cttttgtcaa ggagaaaggt tttccatcct tatcagctca agtcttacgt gaacagttta
    29941 ttctaagaca aatagaaaac aaggtcgatt tgtacgaaat tgcaaaaaaa ttaggattaa
    30001 aaacagtcct gaccttagaa aaatatagat aatggatatt aaattaaaag attttgaagg
    30061 acccctggac ttgctcttgc atctggtttc taagtaccag atggatatct acgatgtgcc
    30121 cattacggaa gtcatcgaac agtatctagc ctatgtctca accctgcagg ccatgcgtct
    30181 ggaagtgacg ggtgagtaca tggtcatggc tagtcagctc atgctgatta agagtcgtaa
    30241 actccttccg aaggtagcag aagtgacaga cttgggggat gacctggagc aggacctcct
    30301 ctctcaaatc gaagaatatc gcaagttcaa gctcttgggt gagcacttgg aagccaagca
    30361 ccaagaacgg gcccagtatt attccaaagc gccgacagag ttgatttacg aagatgcgga
    30421 gcttgtgcat gacaagacga ccattgacct ctttttggct ttttcaaata tcctagccaa
    30481 gaaaaaagag gagtttgcac aaaatcacac gacgatcttg cgggatgagt ataagattga
    30541 ggacatgatg attatcgtga aagagtcctt gattggacga gatcaattgc gcttgcagga
    30601 tttgttcaag gaagcccaga atgtccaaga ggtcatcacc ctctttttgg caaccctaga
    30661 gttaatcaaa acccaggagc tgatcctcgt gcaagaggag agttttggag atatctatct
    30721 catggaaaag aaggaagaaa gtcaagtgcc tcaaagctag acttgataga gaggaaagat
    30781 gagtacttta gcaaaaatag aagcgctctt gtttgtagcg ggtgaagatg ggattcgggt
    30841 ccgccagtta gctgaactcc tctctctgcc accgacaggc atccagcaaa gtttaggaaa
    30901 attagcccag aagtatgaaa aggacccaga ttccagtttg gctttgattg agacaagtgg
    30961 tgcttataga ttggtgacca agcctcaatt tgcagaaatt ttgaaggaat actctaaggc
    31021 gcctatcaac cagagcttgt ctcgggctgc ccttgagacc ttgtccatta ttgcctacaa
    31081 acagccgatt acgcggatag aaattgatgc catccgtggg gttaactcga gtggagcctt
    31141 ggcaaagttg caggcttttg acctgataaa ggaagacggg aaaaaggaag tattggggcg
    31201 ccccaacctc tatgtgacta cggattattt cctagattac atggggataa accatttaga
    31261 agaattacca gtgattgatg agcttgagat tcaagcccaa gaaagccaat tatttggtga
    31321 aaggatagaa gaagatgaga atcaataagt atattgccca cgcaggtgtg gccagtagga
    31381 gaaaagcaga agagctgatt aagcaaggct tggtgacggt taacggccaa gtggtgcgtg
    31441 aactagcaac cactatcaag tcaggcgaca aggtcgaagt tgaaggtcaa cctatctaca
    31501 acgaagaaaa ggtctactat ctgcttaaca aaccacgcgg tgtgatttcc agtgtgacag
    31561 atgataaggg tcgcaagacg gttgtcgacc tcttgcccaa tgtcaaagaa cgtatttacc
    31621 ctgtgggtcg tttggactgg gatacatcag gtgtcttgat tttgaccaat gatggggact
    31681 ttacagacga gatgattcac cctcgtaatg agattgacaa ggtttatgtc gcgcgtgtta
    31741 aaggtgtggc caataaggac aatctccgcc ccttgacccg tggtcttgag attgatggta
    31801 agaaaaccaa gccagctgtt tatgaaattc tcaaagtgga cccagtcaaa aatcgctctg
    31861 tggtgcagtt gaccatccat gaagggcgta accatcaggt taaaaagatg tttgaagctg
    31921 ttggtctcca agtagataag ttgtctcgga ctcgtttcgg acacctagac ttgacaggac
    31981 tccgtccagg agaatcccgt cgtcttaata aaaaagaaat cagccaacta cacaccatgg
    32041 ctgtaactaa gaaataatga aacgaatttt aatagcgcct gtgcgctttt accaacgttt
    32101 tatctcacca gtctttccac cctcttgtcg ctttgagctg acttgttcca actacatgat
    32161 tcaggctatt gaaaaacatg gttttaaggg ggtattgatg ggcttggctc ggattttacg
    32221 ttgtcatccc tggtcgaaaa caggtaagga ccccgttcca gaccactttt cccttaaacg
    32281 aaatcaagaa ggggaatgag gtggggtaaa tagatttcaa aatgataaaa acgcatccta
    32341 tcaggtttga gtgaatttga taggatgcgt tttagaatgt caaaatttta tactcttcga
    32401 aaatcccttc aaaccgcgtc tgctttcatc tgcaacctca aaacagtgtt ttgagcaacc
    32461 tgcggctagt ttcctagttt gctctttgat tttcattgag tattaaattg agtttgaagt
    32521 ggcttatttc aaagcttttt gtatgtcttc aatcatgagt tttgttgatt caagtccgcc
    32581 tccgcttaga taccagaggt ctggtgttag ttggataatc ttaccatttt tagcagcagg
    32641 tgtttcagcg ataagggcat tttctaggac accgtcgttg ctagagttgt ccccaccgat
    32701 ggcaagggta cggttgatga caaagaggat gtcagggttg atttctttga cactttcaaa
    32761 gctgacttct tgtccgtggc gtgagtcttc aaatgttgta tcagttggtt tgaatttcaa
    32821 ggtttggtac aagaaagaga aacgagattt ggcaccaaag gctgccattt ttccttcatt
    32881 aaggaggatc gcaagggcct ttttgtcaga gctttcattt ttagtagcga cttcttggat
    32941 gctcttgtct agcttggcca attcttcctt ggctttctgt gtaccagttt cgccgaaggc
    33001 acttgctaag gattcgatat tagccttggt agaagtccag tagtcgtcct tgcttgcttg
    33061 gaagagaacg gttggggcga tttctttgaa tttgtctacg aatttttgtg tacgtggtga
    33121 agcgataatc aaatcaggct caagggcggc gatagcttct aaatcaggtt ctttcataga
    33181 accaacattt ttgacagttc ccactaggtc ttttagataa gtcggaacag tttttgtagg
    33241 cattccgacg atattttttt caaatcctaa agcgcgaata gtatccgcag cgccgaggtc
    33301 aaaggtcaca atcttttcag gaactttgga aagtttgacc tcgtccagtg aacttttaat
    33361 ggttacctct gttggagcag agctactggt ctctgtctga ctagtgcttg agtttgtact
    33421 acatgcacca agtaggagca agaagctggc cactagggca gtgaaataaa gtttaaggga
    33481 tgttttcata atttctcctt tttaaaatgt gataacgatt tagggagtct cttgatctta
    33541 ttgattaaga gactgaaggt tctctaactt gagcttttat gttactagct atagatacag
    33601 atctttttgt cattgatatc agctagcgtg atgggaatct cataaagttg gctgagcagg
    33661 tcagcctgca tgatttgatc ggtgcttccc ttgctaaaga cctgaccgtc cttgaaggcg
    33721 acaatttcat ctgcatactg actggccatg ttgatatcgt ggaggacgat gataatggtc
    33781 ttgccgagtt cctccaccag tcgtcgaaga atttgcatca tgctgacgct ttgcttgata
    33841 tcgagattgt tgagtggttc gtccagcaag ataaagtccg tatcctgggc cagtaccata
    33901 gcgataaaga cgcgctggag ttgcccccct gacaggctat tgatgtagcg gtcttttaag
    33961 ttggtcagtt ccaaatagtt cagagtttct cggatttttt cccagtcttc tgatctaagt
    34021 cgacctcggc tgtagggaaa acgtccaaaa ctgaccagtt cttcaacagt caatttggct
    34081 tggtaattga ttttctgttt taggatggtt agttcttggg ccagttcttg cgaattccag
    34141 ctctcgattt cacgtccttt gatactgaga actccctgat ctttcttggt tagtctgctc
    34201 atgatggaga ggagagtcga ttttccagca ccatttggac caataaaggc tgtcagtttt
    34261 tgaggactga cttcaagcga aatgccttgc aaaatatcct gtttttgaat ggatttgtca
    34321 atgttttcca gtttcactga cgagccctcc tatatagtaa gataaagaat aagaagccac
    34381 ccacactctc aatgatcata ctgatacgaa tttccagtgc aaagactcgt tcaatcaagg
    34441 cttgccccaa ggttaagcta ataaatccaa ccagaatggc cactataaag agtaacttgt
    34501 gctgatagtc tttgacaatc aggtaggtga ggttggccag cataaagccg aagaaggcca
    34561 taggtcctac caaggcagtg gccgttgagg tcaaaagcac gattccccag aggagctctt
    34621 tctgttcttt ttcaacatcg agtcccaata tctgagccgt ttctctttgc aggtgcaaga
    34681 catctagaac gactgctttt cgaaagaaaa agattgtcaa agcgaggatg atcagagaac
    34741 cgatggctag gatggaagtg ttaagatgtt gaaaggaggc aaaaagacta ttttgcagtt
    34801 tatcgtattc gtttggatcc attaggactt gaaggaaggt gctgatattt cgaaagagac
    34861 ttctgagcgc tagacagatc agcaggatga agaccaggtc ttgcttcatc agtgtcttca
    34921 agtaaccttg taaggcgaga aagaagaggg actggacaag aagtaagact aggaattcta
    34981 agatagggga tttgccaagt tgaagaaact tgctttcaaa aaccagtagt agggtttgta
    35041 gtaggacgta gaaggattca attcccaaaa tactaggcgt caggaagcga ttttccgtca
    35101 gggtttgaaa actaatggtc gaaatcccag tcgcgatggc taccaagaga taaacgatga
    35161 tcttttggga acgcaacttc caagcaaagg ctgacaagtg agtgatgggc caaaagtaga
    35221 gaagacaagc tccgatggca agaataatga gaatccagaa gagcttggta tgtttgcttt
    35281 tagtctgcat cttttcgtcc ccctctccag agaagtagga taaagacgag actaccgatg
    35341 attcctagca agagactgac agacaactca tagggcctaa tcagaactcg ggataggata
    35401 tcgcaagcca gaactagatt ggcaccaacc agtgcgacca tgagtttggt ttgacttaga
    35461 ttatctccat agcgcttgcg aacaagattg ggaacgataa ctccgagaaa tggtaggcca
    35521 cccacggtaa tcatggtgac gcttgtcgtt agcgccacca gaaagagggc cagtttttca
    35581 agtagggagt aggaaatccc caaactctcg ctggtttctt tccctagatt catgatggtg
    35641 aaggtttggg ataatttcca aacggttatc aggatgatga ggcctaagaa gagccactca
    35701 tactgatggg tctgaatcat ggagaaggag ccctgggtcc aggcagtcat actctgaacc
    35761 agattgaaac gataggcgat aacttctgtg actgagccga taatcccgct atagatgatc
    35821 ccaatcagag gcaacatcca cctttccttt acagtaaaaa tggtcataaa ggctaggaag
    35881 aagagggtga atacgatgga tgaaacaaaa gcgaagagca tcttttgggt cagactagcc
    35941 gatggaaaga caaaaaggct cagcaccatt cccagtttgg cggcttcagt cgttccaact
    36001 gtactcggtg cagcaaactg attttgggta atagtctgca tgagaaggcc tgccatactc
    36061 atactagagg cagtcaggag aatactgata gttcttggga gacgggactc ttgaaagagg
    36121 agccaggtct gctggtcgaa atcaaatagc tttccccatg aaaaatcact ggtcccaatg
    36181 ctaatagaga gaaagactag gagtagaagt aagccaatta aataatgaga aagtttcata
    36241 ccccgtcctt tcatgtagat ttggtatcga aagatatctg cggatataaa tgtaacatta
    36301 tttttctaat ctgtcaataa aatttctgac aatttaataa atacaacaag gagagagcaa
    36361 caagactttc tcctttgtta tcctattcta aaatgttttt accttaatct gataaaataa
    36421 tatcttcgag ggagtagcta gccgtccaat caagatattg tttagctttt gaagcatctg
    36481 ctaggacact ggctgggtca ctagcacgtc gagcaacaat ctcgtgtggg attttttaat
    36541 ttagtaattc ttcagcagtt ttaaagattt ctttgatagt atagcctttt ttagttccta
    36601 agttaaagat ttgagaagaa ctgtcttctt gaaataggta gttcattcct ttaacatgag
    36661 cctatgcaag gtccaagaca taaatgtaat ctcgaataca tgaaccgtca cgtgtatcgt
    36721 agtcatctcc aaatattttt aagctatcat tttgtcccaa tgcggtcttg ttgatatttg
    36781 gaatgatgtg agttggattt ttcacacgca gaccgtttga agcatccatt tcagccccag
    36841 caacattaaa gtaacggaaa ataacatatt tccagtcgta gcgattggcc atccagtaaa
    36901 tcattcgttc gcccatcagt tttgtctctg cataagggtt gacagggtcg agcagggtat
    36961 cttcagtcac cggcttgtca atacagttat ttccatagag agaagcagtc gaagagaaca
    37021 tgattttttg aatgccaact tcagataaga ctttgagaac ttggttcata ccagcaacgt
    37081 tggcagtgaa gtatttactt ggattttcaa tactttcgct cacaacaatt tcacctgcac
    37141 aatggaggac agcatcaatc tgattttctt ctaagtaagc ctttaaggcg ctagcatcat
    37201 aaacatccag ttctttaaag ctagcacgac tacccacagc cgctcgattt cctgtggaga
    37261 gattatcgag gacatgcacc ttataaccag catttaaaag agctttaacg gtatgggagc
    37321 caatgtagct agtcccacct gtaacaagaa ttgttttggt catgattttt tccttttcta
    37381 gttttgtact atagtaaatt gaaataagat atgaacaact
//
LOCUS       DAVKJU010000016        35934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVKJU010000016 DAVKJU010000000
VERSION     DAVKJU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.016"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..742)
                     /locus_tag="VW191_000698"
                     /pseudo
     CDS             complement(1..742)
                     /locus_tag="VW191_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            737..991
                     /locus_tag="VW191_000699"
     CDS             737..991
                     /locus_tag="VW191_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU9623494.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSGKIKYHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1185..2519
                     /gene="trmFO"
                     /locus_tag="VW191_000700"
     CDS             1185..2519
                     /gene="trmFO"
                     /locus_tag="VW191_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU9623495.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2603..3340
                     /locus_tag="VW191_000701"
     CDS             2603..3340
                     /locus_tag="VW191_000701"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU9623496.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3349..3906
                     /gene="frr"
                     /locus_tag="VW191_000702"
     CDS             3349..3906
                     /gene="frr"
                     /locus_tag="VW191_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU9623497.1"
                     /translation="MANIIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3966..4820
                     /gene="cvfB"
                     /locus_tag="VW191_000703"
     CDS             3966..4820
                     /gene="cvfB"
                     /locus_tag="VW191_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEU9623498.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4829..5044
                     /locus_tag="VW191_000704"
     CDS             4829..5044
                     /locus_tag="VW191_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEU9623499.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5130..6098
                     /locus_tag="VW191_000705"
     CDS             5130..6098
                     /locus_tag="VW191_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU9623500.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(6076..6255)
                     /locus_tag="VW191_000706"
     CDS             complement(6076..6255)
                     /locus_tag="VW191_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623501.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLKPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            6291..6791
                     /locus_tag="VW191_000707"
     CDS             6291..6791
                     /locus_tag="VW191_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623502.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            6794..7053
                     /locus_tag="VW191_000708"
                     /pseudo
     CDS             6794..7053
                     /locus_tag="VW191_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7420..7746)
                     /locus_tag="VW191_000709"
     CDS             complement(7420..7746)
                     /locus_tag="VW191_000709"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623503.1"
                     /translation="MVDVAVDQGGVIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(7692..8105)
                     /locus_tag="VW191_000710"
     CDS             complement(7692..8105)
                     /locus_tag="VW191_000710"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEU9623504.1"
                     /translation="MGAHFLTKQAGGSGVLPGGVPGVPKGKVTIIGDGVVGTHAARIA
                     LGLGAQVTILDISAKRLSVLEEVFGSQIQTLISNSFNIEASMRDADVVIGAILIPGAK
                     APELVTDEMVKQMRPGSVSLTLLLTKVALSKQLTV"
     gene            complement(8106..8531)
                     /locus_tag="VW191_000711"
     CDS             complement(8106..8531)
                     /locus_tag="VW191_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU9623505.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLSSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            8708..9277
                     /locus_tag="VW191_000712"
     CDS             8708..9277
                     /locus_tag="VW191_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623506.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKDRIVTRVHYVLTKEDYFANK"
     gene            9345..9995
                     /locus_tag="VW191_000713"
     CDS             9345..9995
                     /locus_tag="VW191_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEU9623507.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTNGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9979..12219
                     /locus_tag="VW191_000714"
     CDS             9979..12219
                     /locus_tag="VW191_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU9623508.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFFTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFIFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNTWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPYQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12398..12586
                     /locus_tag="VW191_000715"
     CDS             12398..12586
                     /locus_tag="VW191_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623509.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12618..13094
                     /locus_tag="VW191_000716"
     CDS             12618..13094
                     /locus_tag="VW191_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9623510.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLA"
     gene            13209..14390
                     /locus_tag="VW191_000717"
     CDS             13209..14390
                     /locus_tag="VW191_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623511.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVS
                     LERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPS
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     misc_feature    14550..14681
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14697..15227
                     /gene="infC"
                     /locus_tag="VW191_000718"
     CDS             14697..15227
                     /gene="infC"
                     /locus_tag="VW191_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU9623512.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            15260..15460
                     /gene="rpmI"
                     /locus_tag="VW191_000719"
     CDS             15260..15460
                     /gene="rpmI"
                     /locus_tag="VW191_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU9623513.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15512..15871
                     /gene="rplT"
                     /locus_tag="VW191_000720"
     CDS             15512..15871
                     /gene="rplT"
                     /locus_tag="VW191_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU9623514.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15929..16309
                     /locus_tag="VW191_000721"
     CDS             15929..16309
                     /locus_tag="VW191_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9623515.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16559..17359
                     /locus_tag="VW191_000722"
     CDS             16559..17359
                     /locus_tag="VW191_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEU9623516.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPENETVSQRVCEDGPVFRTGTVVL"
     gene            17370..18308
                     /locus_tag="VW191_000723"
     CDS             17370..18308
                     /locus_tag="VW191_000723"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEU9623517.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18350..20383
                     /locus_tag="VW191_000724"
     CDS             18350..20383
                     /locus_tag="VW191_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEU9623518.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGAIGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20444..22099)
                     /gene="pavA"
                     /locus_tag="VW191_000725"
     CDS             complement(20444..22099)
                     /gene="pavA"
                     /locus_tag="VW191_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEU9623519.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRKQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            22204..22701
                     /gene="ybeY"
                     /locus_tag="VW191_000726"
     CDS             22204..22701
                     /gene="ybeY"
                     /locus_tag="VW191_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU9623520.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22682..23077
                     /locus_tag="VW191_000727"
     CDS             22682..23077
                     /locus_tag="VW191_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEU9623521.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            23094..23993
                     /gene="era"
                     /locus_tag="VW191_000728"
     CDS             23094..23993
                     /gene="era"
                     /locus_tag="VW191_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU9623522.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            24041..24865
                     /gene="mutM"
                     /locus_tag="VW191_000729"
     CDS             24041..24865
                     /gene="mutM"
                     /locus_tag="VW191_000729"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEU9623523.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24865..25470
                     /locus_tag="VW191_000730"
     CDS             24865..25470
                     /locus_tag="VW191_000730"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU9623524.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDTQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25457..26656
                     /locus_tag="VW191_000731"
     CDS             25457..26656
                     /locus_tag="VW191_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEU9623525.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26656..26805
                     /gene="rpmG"
                     /locus_tag="VW191_000732"
     CDS             26656..26805
                     /gene="rpmG"
                     /locus_tag="VW191_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9623526.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26845..27078
                     /gene="secG"
                     /locus_tag="VW191_000733"
     CDS             26845..27078
                     /gene="secG"
                     /locus_tag="VW191_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU9623527.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            27180..29534
                     /gene="rnr"
                     /locus_tag="VW191_000734"
     CDS             27180..29534
                     /gene="rnr"
                     /locus_tag="VW191_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU9623528.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     AGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDCLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29497..29964
                     /gene="smpB"
                     /locus_tag="VW191_000735"
     CDS             29497..29964
                     /gene="smpB"
                     /locus_tag="VW191_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU9623529.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29980..30840
                     /gene="tehB"
                     /locus_tag="VW191_000736"
     CDS             29980..30840
                     /gene="tehB"
                     /locus_tag="VW191_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEU9623530.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30913..31866
                     /gene="coiA"
                     /locus_tag="VW191_000737"
     CDS             30913..31866
                     /gene="coiA"
                     /locus_tag="VW191_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEU9623531.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31885..33687
                     /gene="pepF"
                     /locus_tag="VW191_000738"
     CDS             31885..33687
                     /gene="pepF"
                     /locus_tag="VW191_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9623532.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHLYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33689..34402
                     /locus_tag="VW191_000739"
     CDS             33689..34402
                     /locus_tag="VW191_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU9623533.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34469..35410
                     /gene="prsA"
                     /locus_tag="VW191_000740"
     CDS             34469..35410
                     /gene="prsA"
                     /locus_tag="VW191_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEU9623534.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35528..35869)
                     /locus_tag="VW191_000741"
                     /pseudo
     CDS             complement(35528..35869)
                     /locus_tag="VW191_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10776 a         6683 c         8077 g        10398 t
ORIGIN      
        1 cgatgatttc gataggttgt tgaaaacatt ttaaacgttt ttactttggc aaagatgttc
       61 tcaaccttgc ttctctcctt ggatagcgca tggttacagg ctttatcttc agctgttagc
      121 ggcttgagtt tgctggattt acgtggagtt tgtgcttgag gatatatctt catgagccct
      181 tgataaccac tgtcagccaa gattttacca gcttgtccga tatttctgcg actcattttg
      241 aacaacttca tatcataaca aatagttcac agcgatatcc aaagaaacaa ttctcccttg
      301 acttgtgaca atcgcttgag ccttcatagc gtggcatttc tttttaccag aatcattcgc
      361 taattctttt tttagggcga ttgatttgta cttctgtcgc atcaatcatt accgtgtcct
      421 cagagctgag aggagttctt gaaatcgtaa caccactttg aacaagagtt acttcaaccc
      481 attggctccg acggattaag ttgctttcgt ggataccaaa atcagccgca atttcttcat
      541 aagtgcgata ttctcgcaca tattgaagag tagccataag aaggtcttct aggcttaatt
      601 taggttttcg tccacctttt gcgtgtttaa gttgataagc tgtttttaat acagctaaca
      661 tctcttcaaa agtcgtgcgc tgaacaccaa caaaacgctt aaatcgtgca tcagttagtt
      721 gtttacttgc ttcatcattc atagaactac tataccatat tttgtttcgc aggaagtcta
      781 ttggaaagta agaaatattg aagctgaggc tattagaaga aattgtgagc gtggtgctat
      841 tttttcaggt aaaataaaat atcacgaaga ttcacagttt aaaggagatc actatgttga
      901 atgttatgct gttttagata atacggttat agcaagagat agaataacag tccctatcga
      961 tccgttatgt ggaaaagatt ttatagagta gcatataatt gattcttaac tggaatactc
     1021 actatctctt tacatcaaga aaatgactaa acagggaagt ttgccttctt cccttttttt
     1081 gttatactag tagaagaaaa aattagaaag atttgtgggt gtcaaacagt ccagtggggt
     1141 gttttaatat ggacttaggt cccacccaaa gaggtattag tgtcgtgtct caatcttata
     1201 tcaatgttat cggtgctggt ttggcaggtt ctgaagcagc ttaccaaatc gcagagcgtg
     1261 gtattccagt taaactatat gaaatgcgtg gtgtcaagtc tacaccccag cataaaacag
     1321 acaattttgc tgagttggtt tgttccaatt ctttgcgtgg ggatgctttg acaaatgcag
     1381 ttggtcttct caaggaagaa atgcgtcgct tgggttctgt tatcttggaa tctgctgagg
     1441 ctacacgtgt tcctgccggt ggagcccttg cggtagatcg tgatggtttc tcccaaatgg
     1501 taaccgaaaa agtggtcaac caccccttga ttgaagtggt gcgtgatgaa attacagaat
     1561 tgccaacaga tgttattaca gttgtggcga ccggtccctt gactagcgat gccctggctg
     1621 aaaagattca tgcccttaat gatggcgatg gcttctattt ctacgatgca gcagcgccta
     1681 ttatcgatgt caacactatc gatatgagta aggtctatct caaatctcgt tatgataagg
     1741 gagaagcagc ctatctcaat gctccaatga ccaagcaaga gtttatggat ttccatgaag
     1801 ccttggtcaa tgcggaagaa gcaccgctca attcttttga aaaagaaaag tactttgaag
     1861 gatgtatgcc aatcgaagtt atggccaaac gtggcattaa aactatgctt tatggcccta
     1921 tgaagccagt cggtcttgag tatccggacg actacacagg acctcgtgat ggagaattta
     1981 aaacacctta tgcggttgtg caacttcgtc aggataatgc agctggtagc ctctacaata
     2041 tcgttggttt ccagacccac ctcaaatggg gagaacaaaa gcgtgtcttc caaatgattc
     2101 cgggtcttga aaatgcggag tttgtccgtt atggtgtcat gcatcgcaat tcttacatgg
     2161 attcaccaaa tcttcttgag cagacttacc gttccaagaa acaaccaaat ctcttctttg
     2221 ctggtcagat gacgggggtg gaaggctatg ttgagtcggc ggcttcgggc ttagttgcgg
     2281 gaattaacgc agctcgtctc ttcaaggaag aaagcgaggt tattttcccc gagacgacag
     2341 cgattggaag cttagctcat tacattaccc atgccgacag caaacatttc caaccaatga
     2401 atgtcaattt tgggatcatc aaggagttgg aaggcgagcg tatccgtgat aagaaggctc
     2461 gttatgaaaa aattgcagag cgtgcccttg ccgacttaga ggaatttttg actgtctaat
     2521 ttttttgaaa gaattgctca tgatactata aaaatcttag aaattgtgat aaaataggta
     2581 ggatgaaaga aggagagtga aaatggcgaa tcccaagtat aaacgtattt taatcaagtt
     2641 atcaggtgaa gcccttgccg gtgaacgtgg cgtagggatt gatatccaaa cagttcaaac
     2701 aatcgcaaaa gagattcaag aagttcatag cttaggtatc gaaattgccc ttgttattgg
     2761 tggaggaaat ctctggcgtg gagaacctgc agcagaagca ggtatggacc gtgttcaggc
     2821 agattacact ggaatgcttg ggactgttat gaatgctctt gtgatggcag attcattgca
     2881 acaagttggg gttgatacgc gtgtacaaac agctattgct atgcaacaag tggcagagcc
     2941 ttatgtccgt ggacgtgccc ttcgtcacct tgaaaaaggc cgtatcgtta tctttggtgc
     3001 tggaattggt tcaccatact tctcgacaga tacaacagcg gcccttcgcg cagctgaaat
     3061 cgaagcagat gccatcctca tggctaaaaa tggcgtcgat ggtgtgtaca atgccgatcc
     3121 taagaaggac aagacagccg ttaagtttga agaattgacc caccgtgatg ttatcaacaa
     3181 aggtcttcgt atcatggact caacagcctc aaccctctca atggacaacg acattgactt
     3241 ggttgtcttc aacatgaacc aatcaggcaa catcaaacgt gtcgtatttg gtgaaaatat
     3301 cggaacaaca gtttcaaata atatcgaaga aaaggaataa gaaagaatat ggctaacata
     3361 attattgaaa aagctaaaga gagaatgacc cagtctcacc aatcacttgc tcgtgaattt
     3421 ggtggtatcc gtgctggtcg tgccaatgca agcttgcttg accgtgtaca tgtagaatac
     3481 tatggagtcg aaactcctct taaccaaatc gcttcaatta cgattccaga agcgcgtgtt
     3541 ttgttggtaa caccatttga caagtcttca ttgaaagaca tcgaacgtgc cttgaacgct
     3601 tctgatcttg gtatcacacc ggctaatgac ggttctgtga ttcgcttggt tatcccagct
     3661 cttacagaag aaactcgtcg tgaccttgct aaagaagtga agaaggtcgg cgaaaatgct
     3721 aaagtggctg tccgcaatat ccgtcgcgat gctatggacg aagctaagaa acaagaaaaa
     3781 gcaaaagaaa tcactgaaga cgaattgaag actcttgaaa aagatattca aaaagtaaca
     3841 gacgatgctg ttaaacacat cgacgacatg actgctaaca aagagaaaga acttttggaa
     3901 gtctaaaaat aaacagaaaa actcagttgg cattgctggc tgagttttat tcgaaagaag
     3961 gaaatatgaa tacaaatctt gcaagtttta tcgttggact gatcatcgat gaaaatgacc
     4021 gtttttactt tgtgcaaaag gatggtcaaa cctatgctct tgctaaggaa gagggccaac
     4081 atacagtagg ggatacggtc aaaggttttg catacacgga tatgaagcaa aaactccgcc
     4141 tgacaacctt agaagtgact gccactcagg accaatttgg ttggggacgt gtcacagagg
     4201 ttcgtaagga cttgggtgtc tttgtggata caggccttcc tgacaaggaa atcgttgtgt
     4261 cactcgatat tctccctgag ctcaaggaac tctggcctaa gaagggcgac caactctaca
     4321 tccgtcttga agtggataag aaagaccgta tctggggcct cttggcttat caagaagact
     4381 tccaacgtct cgctcgtcct gcctacaaca acatgcagaa ccaaaactgg ccagccattg
     4441 tttaccgtct caagctgtca ggaacttttg tttacctacc agaaaataat atgcttggtt
     4501 ttattcatcc tagcgagcgt tacgcagagc cacgtttggg gcaagtatta gatgcgcgcg
     4561 ttattggttt ccgtgaagtg gaccgcactt tgaacctctc cctcaaacca cgctcctttg
     4621 aaatgttgga aaacgatgct cagatgattt tgacttattt ggaaagcaat ggcggtttca
     4681 tgaccttaaa tgacaagtca tctccagacg acatcaaggc aacctttggc atttctaaag
     4741 gtcagttcaa gaaagcttta ggtggtctta tgaaggctgg taaaatcaag caggaccagt
     4801 ttggtacaga gttgatttag ggaggcttat gagaaaatca ttttacactt ggctcatgac
     4861 cgagcgcaat cctaaaagta acagtcccaa agcaattttg gcagacctcg cttttgaaga
     4921 ggcagctttt ccaaaacata cagatgattt tgatgaggtc agtcgctttt tggaggagca
     4981 tgccagtttc tcttttaacc taggagattt tgacagcatt tggcaggaat atctagaaca
     5041 ctagcattta ttcattgggt ttgggctagt aatttctcca tccctctgct ataataaaaa
     5101 gaaataaaag gattagagag gttctttatt tgaaggaaca ttcaatagac attcaactga
     5161 gtcatccaga tgacctgttt catctttttg gttccaatga gcgccatctt cgtttgatgg
     5221 aagaagagct ggatgtggtg attcatgctc gtacggagat tgtccaggtt ttgggagaag
     5281 agtctgcttg tgaggaagcc cgtcaggtta ttcaagctct catggtcttg gtgaaccgtg
     5341 ggatgaccgt tggtacgcca gatgtggtga ctgcaattag catggtcaaa aacgatgaaa
     5401 ttgataagtt tgtcgccctt tacgaagaag aaattatcaa agataatact gggaaaccga
     5461 tccgcgtcaa aaccttaggt caaaaacttt atgtggacag tgtcaaacag catgatgtga
     5521 cctttggaat tgggcctgca gggacgggga agacctttct tgcagttact ttggcagtga
     5581 ctgcccttaa acgtgggcaa gtcaagcgga ttatcttgac acgtccagcg gtggaagcag
     5641 gagagagtct aggttttctt ccgggtgatc ttaaggagaa ggtagatcct taccttagac
     5701 ctgtttatga tgccttgtat cagattctgg ggaaagacca aacaactcga ctcatggagc
     5761 gtgaaattat cgagattgcg ccccttgcct atatgcgtgg acggaccttg gatgatgcct
     5821 ttgtcattct cgatgaggcg caaaatacga ccatcatgca gatgaagatg ttcttgactc
     5881 gtttaggttt tcattctaag atgattgtca atggagatat tagtcagatt gacctgccac
     5941 gtaatgtcaa gtccggtttg attgatgctc aagagaaact caagaacatc catcagattg
     6001 actttgttca tttttcagcc aaggatgtgg ttcgccatcc tgttgtcgct cagattatcc
     6061 gagcctatga atattctact gaagttgcac acgactgatt ttgaggaagt tcgcctgcaa
     6121 aagaatatac ttttgagaga ggaaaatcca gttgtatagg ctaaaggttt tatccaaagg
     6181 tttgagacaa cgattaggca cgatggaaag aacttttatg tggctgatga cgatcagtgc
     6241 atcttcctgt gtcataatca cagggcacaa gaaagtagga atttgaaaag atgattgacc
     6301 aactatctaa gtattacagt tgtaggatac taactgaaaa ggatattcca agtattttat
     6361 ctttatatga aagtaatcct ctgtattttc agcattgtcc accagagcca aattttgcaa
     6421 ctgtaaaaga ggacatgctt tgtctacctg aaggtaaagc taaggctgat aagttttttg
     6481 ttggattttg gaatggattt gaccttgtgg ctgttatgga ttttgtctat gcatatcctg
     6541 atgaggagac tgtttttatt ggtttgttta tggttgatca agcctatcag aggaaaggga
     6601 ttggtagtca tattgtgaca gaagcactag cttattttgc taagaacttt cgaaaggcac
     6661 gtttggctta tgttaaggga aatccgcaat ctcagcattt ttgggaaaag cagggcttta
     6721 aatcaattgg atgcgaggtt aagcaagaac tctatacggt tgttatcgtt gaacagagcc
     6781 tagaagatta gaaatggcat caagtaagaa ctatttggaa tttgttttgg aacaattatc
     6841 aggattagat gatgtgactt accgttccat gatgggggag tatattcttt acttccgcgg
     6901 caagattatt ggcggcattt atgacgatcg cttttagtta aacccgtgca agcagtctta
     6961 gataagattg accaatcttc ttttgagttt ccatacaaag gtgccaaaga aatgatttga
     7021 gtggaagaac ttgataataa gatgtttcta taagacctga ttttagctat gtataaccaa
     7081 ctgccaacgc ccaaacctaa aaagaaaaag caagggtgaa cgaagtaaaa aagaagtctg
     7141 ctaaggccct gtctttgcac gggtaaaatt ttatatataa aaagaagctg ggactaaaga
     7201 gctcaacttc ctttggttta tataattgtc attacaagac gaagtggttg ggcgaaactc
     7261 tgttgacttt attcaattta gagtttctta tgcacaattg agtctggaac gaaagtctcc
     7321 agttgcaaag tatacagtac aataaaccaa cgatgtaata gctgatgaca caaagcacag
     7381 tgggtaggac ttgcgaagtc acccttttct tttcaaaatt tatactaaat cattgatatc
     7441 agtgtagtca cgattaagtc cttgagcaac tggtaggcta gtcaagtaac cttgataagt
     7501 ggtcacacct tgacgcaagc cttcatcttc agagattgct tgtgcgaatc ctttgccagc
     7561 caaagcttcg atataaggaa gagtgacatt ggttagggcg atggttgaag tgcgggcaac
     7621 cgcaccaggg atattggcaa cggcatagtg gagaacaccg tgtttttcat agacgggttc
     7681 atcgtgcgtt gtcacacggt cagctgtttc gataacgcca ccttggtcaa cagcaacgtc
     7741 aacgatacag agcctggacg catttgtttg accatctcat ctgtcaccaa ttccggtgct
     7801 tttgcaccag ggatgagaat ggctccaatc accacatcag catctctcat acttgcttca
     7861 atgttgaatg aattagatat aagagtttga atttgacttc caaagacttc ttctagaact
     7921 gagagacgct tggcactaat atctaaaata gtcacttgag caccaagacc aagggcgatg
     7981 cgggcagcat gtgtaccgac gacaccatca ccgatgatag ttacttttcc ttttggaaca
     8041 cctggtacac caccaggtag aacaccagag ccaccagctt gcttagtaag gaagtgagct
     8101 ccgatttaaa cagccatacg acctgcaacc tcactcatag gaacgaggag cggtagttgt
     8161 ccttgattgt cacgaacagt ttcataggca attcctgttg tttttgctgc taacatagca
     8221 tctgctaatt ctggagcagc ggccatgtgc aagtaggtga agagaagaag atcgtcgcgc
     8281 aagtaaccgt attcagaact taaaggttct tttactttca caaccaactc tgctgcccaa
     8341 gcttcaccag cagtagcgac aatctcagct ccttgctttt gatagtcagc atcagtaaag
     8401 ccagaaccga gaccagcatt tgtttcgata aggacacgat gaccacgact aactaagcta
     8461 tgaacacctg caggtgtgag ggcgacacgg ttttcgttat ttttaatttc ttttgggatt
     8521 ccgattaaca ttgagataac ctacctttca attgacggtc ttgttttggt tgtcacattc
     8581 cagttcataa atcaaaaatg tgacggtttc attgtatatg aaaccgcttc aaaaatcaag
     8641 aaaaacttgt catccaaatt ttttatgcta gactagtgaa aatcaagctt tgattggttg
     8701 gaaaagtatg gaatcaattt ttgtaaaact tgcccagtat ccgtctatag aaacggagcg
     8761 tttattgctt agacctgtaa ctttggatga tgcggaagca atgtttgact atgcctcgga
     8821 caagggtaat acacgttaca cttttccaac caatcaaagc ttggaagaaa ccaagaataa
     8881 cattgctcag ttctatttgg ctaatccctt gggacgttgg ggaatagaac taaaaagcaa
     8941 tggtcagttt attggaacca ttgacttgca caagattgat tctgttctta agaaggcagc
     9001 tattggctac attatcaata aaaagtattg gaatcaagga ttaacgacag aagccaatcg
     9061 tgctgtgatt gagctagctt ttgagaagat agggatgaat aagttgactg cccttcacga
     9121 taaggacaat cctgcgtcag gaaaggtcat ggagaaatca ggcatgcgtt tttcccatgc
     9181 agaaccatat gcttgtatgg accagcatga aaaagatcga atcgttacaa gagttcatta
     9241 tgtcttgacc aaggaagact attttgcaaa taaataagca gttgaaaaga aatttttcga
     9301 ctgttttttc ttcctcttac gaataatcta agagaggaga aaatatggaa gcaattatcg
     9361 agaaaatcaa agagtataaa atcatcgtca tctgtactgg tctgggcttg cttgtaggcg
     9421 gatttttcct gctaaaacca gctccacaaa cacctgtcaa agagacgaat ttgcaggctg
     9481 aagtcgcagc tgtttccaag gatttggtat ccgaaaagga agtgaacaag gaagaaaagg
     9541 aagaacccct tgaacaagat ctaatcacag tagatgtcaa aggtgctgtc aaatcgccag
     9601 ggatttatga cttgcctgta ggtagtcgag tcaatgatgc tgttcagaag gctggtggct
     9661 tgacagagca agcagacagc aagtcgctca atctagctca gaaagttagt gatgaggctc
     9721 tggtttacgt tcctactaat ggagaagaag cagttagcca acagactggt ttggggacag
     9781 cttcttcaat aagcaaggaa aagaaggtca atctcaacaa ggctagtctg gaagaactca
     9841 agcaggtcaa gggactggga ggaaaacgag ctcaggacat tatcgaccat cgtgaggcaa
     9901 atggcaagtt caaatcagta gacgagctca agaaggtctc tggcattggt gccaagacta
     9961 tagaaaagtt aaaagactat gttacagtgg attaagaatt tctctattcc cctaatttac
    10021 ctgagttttc tgttactttg gctttactac gccatttttt caacatccta tcttgctttg
    10081 ttgggatttg tttttctgct agtctgtctc tttttccaat ttccttggaa atctgctagc
    10141 aaagttctag tgatttgtgg aatctttgga ttttggtttg tttttcaaaa ttggcaacag
    10201 agtcaagcga gtcaaaatct ggcggattct gttgaaaggg tacggatttt gcctgatact
    10261 attaaggtta atggtgatag tctatccttt cgtggcaagt ctaacggtcg tgctttccaa
    10321 gtctattata aactccagtc cgaggaggag aaagaagcct ttcaagcttt aaccgacctg
    10381 catgagatag gactagaagg gaagctttcg gagccagaag ggcagagaaa ttttggtggc
    10441 tttaattacc aagcctatct gaagactcag ggaatttacc agactctcaa tatcaaaaca
    10501 atccagtcac ttcaaaagat tggcagttgg gatataggag aaaacttgtc cagtttacgt
    10561 cgaaaggctg tggtttggat taagacgcac tttccagacc ctatgcgcaa ttacatgaca
    10621 ggactcttgc tgggacatct ggacaccgac tttgaggaga tgaatgagct ttattccagt
    10681 ctaggaatta tccacctctt tgccctatct ggcatgcagg taggtttttt catgaatgga
    10741 tttaagaaac ttctcttgcg attgggcttg acccaagaaa agttgaaatg gctgacttat
    10801 cccttttccc ttatctatgc gggactaact ggattttcag catcggttat tcgcagtctc
    10861 ttgcaaaagc tactggctca acatggggtt aagggcttgg ataattttgc cttgacggtg
    10921 cttgtcctct ttattgtcat gccaaacttt ttctttacag caggaggagt cttgtcctgc
    10981 gcttatgctt ttatcctgac catgaccagc aaagaagggg aggggctcaa ggctgttgct
    11041 agtgaaagtc tagtcatctc cttgggcata ttgcccattc tatccttcta ttttgcggaa
    11101 tttcaacctt ggtctatcct tttgaccttt atcttttcct ttctatttga cttgaccttc
    11161 ttaccgctct tgtctatttt atttgtcctt tcctttctct atccagtcat tcagctgaac
    11221 tttatctttg aatggttaga gggcattatt cgcttggtct cgcaggtggc aagtagaccg
    11281 cttgtctttg gacaacccaa tacatggctt ttaatcctat tgttaatttc tttggctttg
    11341 gtctatgatt tgaggaaaaa cattaaaaag ctaacggtat tgtgcttatt gattacaggg
    11401 ctctttctcc tgaccaagca tccactggaa aatgaaatca ccatgctgga tgtggggcaa
    11461 ggagaaagta ttttcctacg ggatgtaact gggaaaacca ttctcataga tgtaggtggt
    11521 aaggcagaat cttataagaa aatcaaaaaa tggcaagaaa agatgacgac cagcaatgcc
    11581 cagcgaagct tgatacccta tctcaaaagt cgaggagtag ctaagattga ccagctaatt
    11641 ttgactaaca cggacaagga gcatgttgga gatttgtcag agatgaccaa ggctttccat
    11701 gtaggggaga ttctagtatc aaaagacagt ctgaaacaga aggaatttgt ggcagaacta
    11761 caggcgactc aaacaaaggt gcgtagtatg atagtagggg agaacttgcc catttttgga
    11821 agtcagttag aagttctatc tccaaggaaa atgggagatg gaggacacga tgatacccta
    11881 gttctgtatg ggaaattctt ggataagcaa tttctcttca cgggaaattt ggaggagaaa
    11941 ggagagaagg acttgctgaa gcactatcca gacttgaaag taaatgtttt gaaagctagc
    12001 caacatggca ataaaaaatc atcaagtcca gcctttctag aaaaactcaa accagagctt
    12061 actcttatct cagttggaaa gagcaatcga atgaaactcc cctatcagga aacattgaca
    12121 cgactggaag gtatcaatag caaagtttat cgaactgacc agcaaggagc tatacgtttt
    12181 aaggggttgg atagttggaa aatcgaaagt gttcgatagg aaggataaat gttgtagatt
    12241 agtgaaataa actaaaaatt tgttgcataa taatgataaa aatggtataa tgaaaacgta
    12301 ttcaatattg aggatataaa atcattaaaa atcagcaaaa gttgttttat tagttagttt
    12361 ataatctatt ggtcttcttc agtccagtgt atctgctgtg acagtcacta aaagttacaa
    12421 gtatgattgg aatacggttt tggaatatag taccaactat cacgaccatc agtatgcttg
    12481 gattccggaa tggtctcgtt attacagcta ttctgagtat aaagttggcg gaggctggaa
    12541 ctacgctcgt tatgaggtca taaactacta tactggaggt tattaatcct taaagagtga
    12601 gaaaaggagg gctggatatg ttaaatctta ctcatgttac cttaaaaacg cgacaagtca
    12661 tcttgcaaga tgcggatttt acctttaaaa agggtaggat ttatggcctt cttgctatca
    12721 atggctcggg aaagacgaca ctattccgag ctatgagcaa gttgcttccc cttagtagtg
    12781 gacacatcgc agttcctcct tctttgtttt attatgagag cgttgaatgg ctggatggaa
    12841 acttaagtgg gatggactac cttcgtctca taaaaaacat ctggaagtca gacctaaact
    12901 tgagagatga aatcgcctac tgggaaatgg ctgactatat cagtcttccc atccgcaagt
    12961 attccttagg gatgaagcaa cgcttggtga ttgctatgta ttttctcagt caggccaaat
    13021 gctggctcat ggatgagatt acaaatggct tagacgagta ttatcgacag aagttttttg
    13081 ataggctagc ataaatcgat agacaagaac agctggttct tttaagttcc cactataagg
    13141 aagagttggt tgatatctgc gatagagtag taaccattca tcaggggcag atagaagagg
    13201 tttagtttat gaaagatgtt agtctatttt tattgaaaaa agttttcaaa agtcgtttaa
    13261 actggattat cttactttta tttgcatctg tactcggtgt taccttttat ttaaatagtc
    13321 agactgcaaa ctcagtcagc ttggagagag agttggaaac tcgtcttgta gaccgtgaga
    13381 gagtcatcaa tgaaaatgaa gagaaactct cccaaatgtc tgataccagc tcggaggaat
    13441 accagtttgc taaaaataat ttagacgtgc aaaaaaatct tttgacgcga aagacagaaa
    13501 ttctgacttt attaaaagaa gggcgctgga aagaggccta ctatctgcag tggcaagatg
    13561 aagagaagaa ttatgaattt gtatcaaatg acccgactgc tagctctggc ttaaaaatgg
    13621 gggttgaccg cgaacggaag atttaccaag ccctgtatcc cttgaacata aaagcacata
    13681 ctttggagtt tccgacccac gggattgatc agattgtctg gattttagag gttatcatcc
    13741 caagcttgtt tgtggttgcg attattttta tgctaacaca actatttgca gaaagatatc
    13801 aaaatcatct ggacacagct cacttatatc ctgtttcaaa agtgacattt gcaatgtctt
    13861 ctcttggagt tggagtgggc tatgtaactg tgctgtttat cggaatctgt ggcttttctt
    13921 ttctagtggg aagtctgata agtggttttg gacagttaga ttatccctac ccgatttata
    13981 gcttagtgaa tcaagaggta actattggaa agatgcagga tgtattattt cctagcttgc
    14041 tcttagcttt cttagccttt atcgtcattg tggaagttgt gtacttgatt gcttactttt
    14101 tcaagcaaaa aatgcctgtc ctctttcttt cactcattgg gattgttggc ttattgtttg
    14161 gcatccaaac aattcagcct cttcaaagga ttgcacatct gattcccttt acttacttgc
    14221 gttcagtgga gattttatct ggaagattat ctaagcagat tgataatgtc gatctaaatt
    14281 ggagcatggg gatggtctta cttccttgcc tgattatcct tttgttagtg gggattctat
    14341 ttatcgaaag atggggaagt tcacgtaaaa aggaagtttt taaagtttag ttttcctata
    14401 gggaagctaa agaaaaacta acatagggag agaatcaact tgattctctc tttttgattc
    14461 gaaaaccaaa ccaaaataca aacacaaact tttcaaaaaa taacttttta tcttgacaag
    14521 agctagaaaa cttggtatca tataaaagtt gagaaaagca gaagtgagag cttctcgcct
    14581 tgtgacatta agttgcctgg ccctacggat gaaaagtttc gaagaaacgc tatcataacg
    14641 tgcgggcttg tatatttacg agtccgctat tgtttttctc taataaaaca aaagaggtga
    14701 aaaccatagc aaagcaagac ttattcatca atgatgagat tcgtgtacgt gaagttcgct
    14761 tgattggtct tgagggagaa cagctaggca tcaagccact cagtgaagcg caagctttgg
    14821 ctgataacgc taatgttgac ctagtattga ttcaacccca agccaaaccg cctgttgcaa
    14881 aaattatgga ctacggtaag ttcaaatttg agtaccagaa gaagcaaaaa gaacaacgta
    14941 aaaaacaaag cgttgttact gtgaaagaag ttcgtctaag tccaactatt gacaagggtg
    15001 actttgatac aaaacttcgt aatgcacgta agttccttga aaaaggaaat aaagttaagg
    15061 tatctattcg ctttaagggt cgtatgatta cccataaaga gattggtgca aaagttttag
    15121 ccgagtttgc tgaagcaact caagatattg ccatcatcga acaacgtgct aaaatggatg
    15181 gacgtcaaat gttcatgcaa ttggcgccag cgactgacaa aaaataattg tcagaaagtt
    15241 aaataaagga gaaaaaatca tgccaaaaca aaaaacacac cgcgcatcag ctaaacgttt
    15301 caaacgtaca ggttctggtg gacttaaacg tttccgtgct tacacttctc accgtttcca
    15361 cggaaaaact aagaaacaac gtcgtcatct tcgtaaagca tctatggtgc attcaggaga
    15421 ttacaaacgt atcaaagcaa tgcttactcg cttgaaataa tagcttgact gtaagtaaac
    15481 aattctagga aatatttgga ggaaataaaa catggcacgt gttaaaggtg gcgttgtatc
    15541 acgcaaacgt cgtaaacgta ttcttaaatt agcaaaaggt tactatggag ctaaacacat
    15601 cttgttccgt actgcaaaag aacaagtaat gaactcttac tactatgcat accgtgaccg
    15661 tcgtcagaaa aaacgtgact tccgcaaatt gtggattact cgtatcaatg cggcagctcg
    15721 tatgaacgga ctttcatact cacaattgat gcatggtttg aaattggctg agattgaagt
    15781 taaccgtaaa atgcttgctg acttggctgt taacgatgca gtagctttca cagctcttgc
    15841 agatgcagct aaagcaaaac ttggtaaata acaactgatg agactggaac aggattgtcc
    15901 agtcttttta aaaataaagg agaaagatat ggcttcaaaa atgctacaca cttgcttacg
    15961 agtagaaaat cttgaaaaat caatcgcatt ttatcaagat gcttttggtt ttaaagaatt
    16021 gcgtcgcaga gattttccag accatgcctt cacgattgtc tatctaggtc ttgagggtga
    16081 tgactatgag ttggagttga cttataacta cgatcacggt ccgtatgtgg ttggtgatgg
    16141 gtttgcccat atcgccctca gtacacctga tcttgaggca cttcatcaag aacacagtgc
    16201 aaaaggctat gaagtgactg agccaaatgg tctaccagga actacaccaa actattactt
    16261 tgtcaaagac cctgatggat ataaggtcga agtcattcgt gaaaaataat ttcgtgcaag
    16321 ttaagtagaa tattcgtttt ctacttgact ttttttagct gaaagagtat actaataaaa
    16381 attcaacctt taaggggagt ccagagagac tcacaaggtg tcagataaaa gaatagtgca
    16441 attttctaga ggagactttt tgagtgtgct ctcttgtgtt gtacgatttt aactgaggcc
    16501 ttgcactagc aaggtctttt ctttatctgg tccccttaaa atttaaggag gaaaagttat
    16561 gaatcccaca tgtaagaagc gtttgggtgt cattcggttg gaaaccatga aggtggttgc
    16621 acaagaggaa atcgcgccag caatctttga attagtccta gaaggagaaa tggttgaagc
    16681 catgcgagca ggccaatttc ttcatctgcg tgtaccggac gatgcccatc tcttacgtcg
    16741 tcctatttca atttcgtcta ttgacaaggc aaacaagcag tgtcacctca tttatcggat
    16801 tgagggagct gggactgcaa ttttttcaac cttaagtcag ggagacactc ttgatgtgat
    16861 ggggcctcag ggaaatggtt ttgacttgtc tgaccttgat gagcagaatc aggttctcct
    16921 tgttggtggt gggattggtg ttccaccctt gcttgaggtg gccaaggaat tgcatgaacg
    16981 tggagtgaaa gtagtgacag tcctcggttt tgctaataag gatgctgtta ttttgaaaac
    17041 ggaattggct cagtatggtc aggtctttgt aacgacagat gatggttctt atggcatcaa
    17101 gggaaatgtt tccgttgtta tcaatgattt agatagtcag tttgatgctg tttactcgtg
    17161 tggggctcca ggaatgatga agtatatcaa tcaaaccttt gatgatcacc caagagccta
    17221 tttatctctg gaatctcgta tggcttgtgg gatgggagct tgctatgcct gtgttctaaa
    17281 agtaccagaa aacgagacgg tcagccaacg cgtctgtgaa gatggtcctg ttttccgcac
    17341 aggaacagtt gtattataag gagaaaatta tgactacaaa tcgattacaa gtttctctac
    17401 ctggtttgga tttgaaaaat ccgattattc cagcatcagg ctgttttggc tttggacaag
    17461 agtatgccaa gtactatgat ttagaccttt taggttctat tatgatcaag gcgacaaccc
    17521 ttgaaccacg ttttgggaat ccaactccaa gagtggcaga gacgcctgct ggtatgctca
    17581 atgcaattgg cttgcaaaat cctggtttag aggttgtttt ggctgaaaag ctaccttggc
    17641 tggaaagaga atatccaaat cttcctatta ttgccaatgt agctggtttt tcaaaacaag
    17701 agtatgcagc tgtttctcat gggatttcca aggcaactaa tgtaaaagct atcgagctca
    17761 atatttcttg tcccaatgtt gaccactgta atcacggact tttgattggt caagatccag
    17821 atttggctta tgatgtggtg aaagcagctg tggaagcctc agaagtgccg gtttatgtca
    17881 aattaacccc gagtgtgacc gatatcgtta ctgtcgcaaa agctgcagaa gatgcgggag
    17941 caagtggctt gaccatgatc aatactctgg ttggaatgcg ctttgacctc aaaaccagaa
    18001 aaccaatctt ggccaatgga acaggtggaa tgtcaggtcc agcagtcttt ccagtagccc
    18061 tcaaactcat ccgccaagta gcccaaacaa cagacctgcc tatcattgga atggggggag
    18121 tggattcggc tgaagctgcc ctagaaatgt atctggctgg ggcatctgct atcggagttg
    18181 gaacagctaa ctttaccaat ccttatgcct gccctgacat catcgaaaat ttaccaaaag
    18241 tcatggataa atacggtatt agcagtctgg aagaactccg tcaggaagta aaagagtctc
    18301 tgaggtaaac tgcaatcaat ctgttcttga ttttttatta gtttgtaata tgaatttagg
    18361 agaattttgg tacaataaaa taaataagaa cagaggaaga aggttaatga agaaagtaag
    18421 atttattttt ttagctctgc tatttttctt agctagtcca gagggtgcaa tggctagtga
    18481 tggtacttgg caaggaaaac agtatctgaa agaagatggc agtcaagcag cgaatgagtg
    18541 ggtttttgat actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga
    18601 aaatgaatgg ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc
    18661 caaatcagaa tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat
    18721 gaaaagaaat gcttgggtag gaacttccta tgttggtgca ataggtgcca aagtaataga
    18781 agactgggtc tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca
    18841 cgcagagaaa gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta
    18901 tctactgaca agtcagtgga ttaatcaagc ttatgtgaat gctagtggtg tcaaagtaca
    18961 gcaaggttgg ctttttgaca aacaatacca atcttggttt tacatcaaag aaaatggaaa
    19021 ctatgctgat aaagaatgga ttttcgagaa tggtcactat tattatctaa aatccggtgg
    19081 ctacatggca gccaatgaat ggatttggga taaggaatct tggttttacc tcaagtttga
    19141 tggcaaaatg gctgaaaaag aatgggtcta cgattctcat agtcaagcct ggtactactt
    19201 caaatccggt ggttacatga cagccaatga atggatttgg gataaggaat cttggtttta
    19261 cctcaaatct gatgggaaaa tagctgaaaa agaatgggtc tacgattctc atagtcaagc
    19321 ttggtactac ttcaaatccg gtggttacat gacagccaat gaatggattt gggataagga
    19381 atcttggttt tacctcaagt ctgatggcaa aatggctgaa aaagaatggg tctacgattc
    19441 tcatagtcaa gcctggtact acttcaaatc tggtggctac atggcgaaaa atgagacagt
    19501 agatggttat cagcttggaa gcgatggtaa atggcttgga ggaaaagcta caaatgaaaa
    19561 tgctgcttac tatcaagtag tgcctgttac agccaatgtt tatgattcag atggtgaaaa
    19621 gctttcctat atatcgcaag gtagtgtcgt atggctagat aaggatagaa aaagtgatga
    19681 caagcgcttg gctattacta tttctggttt gtcaggctat atgaaaacag aagatttaca
    19741 agcgctagat gctagtaagg actttatccc ttattatgag agtgatggcc accgttttta
    19801 tcactatgtg gctcagaatg ctagtatccc agtagcttct catctttctg atatggaagt
    19861 aggcaagaaa tattattcgg cagatggcct gcattttgat ggttttaagc ttgagaatcc
    19921 cttccttttc aaagatttaa cagaggctac aaactacagt gctgaagaat tggataaggt
    19981 atttagtttg ctaaacatta acaatagcct tttggagaac aagggcgcta cttttaagga
    20041 agccgaagaa cattaccata tcaatgctct ttatctcctt gcccatagtg ccctagaaag
    20101 taactgggga agaagtaaaa ttgccaaaga taagaataat ttctttggca ttacagccta
    20161 tgatacgacc ccttaccttt ctgctaagac atttgatgat gtggataagg gaattttagg
    20221 tgcaaccaag tggattaagg aaaattatat cgatagggga agaactttcc ttggaaacaa
    20281 ggcttctggt atgaatgtgg aatatgcttc agacccttat tggggcgaaa aaattgctag
    20341 tgtgatgatg aaaatcaatg agaagctagg tggcaaagat tagtactata agtgaatatg
    20401 atttgagtga atagtaagtt aaaaatcctg atttcaagta aaatcaggat tttttcatgg
    20461 atgcaatttt tttggagtct ggtgtgacgc ggagggtctt ttgtcctgtg taagtgacaa
    20521 agccgggttt tccaccagtt ggtttattga gttttttgac ttcaatcata tctacctgca
    20581 ccagattcga caggcgccct tgagagaagt aggcagctaa ctctgctgcg tctgtcttga
    20641 ctgcatcaga tgggtcaaga tttcctgaga tgacaacatg gcttccagga atgtccttag
    20701 catggaacca aagttcctcc ttgcgggcca ttttaaaggt caattcctca ttttgaagat
    20761 tgtttcgtcc gacatagatg atggttttgc catcgcttgc tagatattgt tctagttttt
    20821 tgcgtttctg gattttctcc cgttgttttc tgcggataaa acctgtttga atcaattctt
    20881 cgcggatttc agcgatttct tccagtccag cttggttgag gacggtttct acactttcca
    20941 gatagagaat agtggctttg gtttcttcaa tcaaatcagt caagtatttg acagcttctt
    21001 tgagtttctg ataccgttta aaatagcgtt gggcattctg gttgggagtc agagccttat
    21061 caagcgcaat catgataggt tggttggtat agtagttgtc taggataacc tggtcttggt
    21121 cgttaggcac ttggtgtagg aaggttgtca gcaattctcc tttttgacga aattcttcag
    21181 cgttgtctgt cgccagtaac tctttttcct gttttttgag tttgtgtcgg tttttctgaa
    21241 gttcattttc aacacgacga atcagttcac tggcctgctg tttgacgcgg tcgcgctcag
    21301 ccttatcctt atagtaggtg tccaacaaat cagaaagatt tgcaaaaggc tctcccacct
    21361 gatttgcaaa aggaactgga ctgaaggaag tctcagtcaa gcatggcttg gtttcttgat
    21421 tgaaaaaatt tcggaaagcg gaaagttttt cactaaccag tatcctttcc aattcatttg
    21481 ccgtatcgcg tcccagacct tgaaagaggc tttgaagatt ttttgctgtt agttcttggg
    21541 tttgcaggat ttcaaagagc ttttcatctt tgatagtaaa aggattgaga gattctgtac
    21601 ttggcggagc gatataggtc gatcctggaa gtaaggtgcg gtagctattt tgtgaaaaac
    21661 cgacgtgttt gataacttcg aggattttat gactgctttt atcgaccagt agaatattac
    21721 tgtgtttccc cataatttca ataatcaagg tagcctggat atggtctcca atctcgtttt
    21781 tattggaaac tgtaatttcc acaatacggt cattttccac ttgctcaatc gactcaatca
    21841 gggccccctg caaatacttt ctcaaaacca tgataaaggt agaaggttga gctggatttt
    21901 caaaagtcgt ttgggtcagc tgaatgcgtc caaaaactgg atgggcagaa aggagcaggc
    21961 gatggctttg gcgattgctg cggatttgca agaccaactc ttgttcaaaa ggctgattga
    22021 ttttctggat gcgaccattc actaattcgc ttcgcaattc ctcaactatg tggtgtaaaa
    22081 aaaatccgtc aaatgacatc gttctctcct tgtgattgta ttccatagta ttatatcaaa
    22141 aaggtagaat aaaatcatgg aaatgtggta taataaagcc aagtaaagag aaacgagaag
    22201 cacatgtata ttgaaatggt agatgaaact ggtcaagttt caaaagaaat gttgcaacaa
    22261 acccaagaaa ttttggaatt tgcagcccaa aaattaggaa aagaagacaa ggagatggca
    22321 gtcacttttg tgaccaatga gcgtagtcat gaacttaatc tggagtaccg tgacaccgac
    22381 cgtccgacag atgtcatcag ccttgagtat aaaccagaat tggaaattgc ctttgacgaa
    22441 gaggatttgc ttgaaaatcc agaattggca gagatgatgt ctgagtttga tgcctatatt
    22501 ggggaattgt tcatctctat cgataaggct catgagcagg ccgaagaata tggtcacagc
    22561 tttgagcgtg agatgggctt cttggcagta cacggctttt tacatattaa cggctatgat
    22621 cactatactc cggaagaaga agcggagatg ttcggtttac aagaagaaat tttgacagcc
    22681 tatggactca caagacaata aacgaaaatg gaaaaatcgt gacttgatat ccagtttaga
    22741 atttgccatc acagggattt ttactgctat caaggaagaa cgaaatatgc gaaaacatgc
    22801 agtgacggct ctagtggtca tccttgcagg ttttgttttt caggtgtcac gaatcgaatg
    22861 gctctttctt ctattgagta ttttcttggt agtagccttt gagattatca actctgctat
    22921 tgaaaatgtg gtggatttgg ccagtcacta tcacttttcc atgctggcta aaaatgccaa
    22981 ggatatggcg gccggcgcgg tattagtggt ttctcttttc gcagccttaa caggcgcatt
    23041 gatttttctc ccacgaatct gggatttatt attttaaaca gtaagaggaa attatgactt
    23101 ttaaatcagg ctttgtagcc attttaggac gtcccaatgt tgggaagtca acctttttaa
    23161 atcacgttat ggggcaaaag attgccatca tgagtgacaa ggcgcagaca acgcgcaata
    23221 aaatcatggg aatttacacg actgataagg agcaaattgt ctttatcgac acaccaggga
    23281 ttcacaaacc taaaacagct ctcggagatt tcatggttga gtctgcctac agtacccttc
    23341 gcgaagtgga caccgttctt ttcatggtgc ctgctgatga agcgcgtggt aagggggacg
    23401 atatgattat cgagcgtctc aaggctgcca aggttcctgt gattttggtg gtgaataaaa
    23461 tcgataaggt ccatccagac cagctcttgt ctcagattga tgacttccgt aatcaaatgg
    23521 actttaagga aattgttcca atctcagccc ttcagggaaa taacgtgtct cgtctagtgg
    23581 atattttgag tgaaaatctg gatgaaggtt tccaatattt cccgtctgat caaatcacag
    23641 accatccaga acgtttctta gtttcagaaa tggttcgcga gaaagtcttg cacctaactc
    23701 gtgaagagat tccgcattct gtagcagtag ttgttgactc tatgaaacga gacgaagaga
    23761 cagacaaggt tcacatccgt gcaaccatca tggtcgagcg cgatagccaa aaagggatta
    23821 tcatcggtaa aggtggcgct atgcttaaga aaatcggtag catggcccgt cgtgatatcg
    23881 aactcatgct aggagacaag gtcttcctag aaacctgggt caaggtcaag aaaaactggc
    23941 gcgataaaaa gctagatttg gctgactttg gctataatga aaaagaatac taagtagagg
    24001 tgggctcatg cctgcttctt gttttacaga aggaggactt atgcctgaat tacctgaggt
    24061 tgaaaccgtt tgtcgtggct tagaaaaatt gattatagga aagaagattt cgagtataga
    24121 aattcgctac cccaagatga ttaagacgga tttggaagag tttcaaaggg aattgcctag
    24181 tcagattatc gagtcaatgg gacgtcgtgg aaaatatttg cttttttatc tgacagacaa
    24241 ggtcttgatt tcccatttgc ggatggaggg caagtatttt tactatccag accaaggacc
    24301 tgaacgcaag catgcccatg ttttctttca ttttgaagat ggtggcacgc ttgtttatga
    24361 ggatgttcgc aagtttggaa ccatggaact cttggtgcct gaccttttag acgcctactt
    24421 tatttctaaa aaattaggtc ctgaaccaag cgaacaagac tttgatttac aggtctttca
    24481 atctgccctt gccaagtcca aaaagcctat caaatcccat ctcctagacc agaccttggt
    24541 agctggactt ggcaatatct atgtggatga ggttctctgg cgagctcagg ttcatccagc
    24601 tagatcttcc cagactttga cagcagaaga agcgacagct attcatgacc aaaccattgc
    24661 tgttttgagc caggctgttg aaaaaggcgg ttccaccatt cggacctata ccaatgcctt
    24721 tggggaagat ggaagcatgc aggattttca tcaggtctat gataagactg gtcaagaatg
    24781 tgtacgctgt ggtaccatca ttgagaaaat tcaactaggc ggacgaggca cccacttttg
    24841 tccaaactgt caaaggaggg actgatgaga aaaatcatcg gaatcactgg gggaattgcc
    24901 tctggtaagt caactgtgac aaattttcta agacagcaag gctttcaagt agtggatgcc
    24961 gacgcagtcg tccaccaact acagaaacct ggtggtcgtc tgtttgaggc tctagtacag
    25021 cactttgggc aagaaatcat tcttgaaaac ggagaactca atcgccctct cctagctagt
    25081 ctcatctttt caaatcctga agagcaaaaa tggtctaagc aaattcaagg ggagattatc
    25141 cgtgaggaac tggctacttt gagagaacag ttggctcaga cagaagagat tttcttcatg
    25201 gatattcccc tactttttga gcaggactac agcgattggt ttgctgagac ttggttggtc
    25261 tatgtggacc gagataccca agtagaacgc ttaatgaaaa gggaccagtt gtccaaggat
    25321 gaagctgagt ctcgtctggc agcccagtgg cctttagaaa aaaagaaaga tttggccagc
    25381 caggttcttg ataataatgg caatcagaac cagcttctta atcaagtgca tatccttctt
    25441 gagggaggta ggcaagatga cagagattaa ctggaaggat aatctgcgca ttgcctggtt
    25501 tggtaatttt ctgacaggag ccagtatttc tttggttgta ccttttatgc ccatcttcgt
    25561 ggaaaatcta ggtgtaggga gtgagcaagt cgctttttat gcaggcttag caatttctgt
    25621 ctctgctatt tccgcggcgc tcttttctcc tatttggggt attcttgctg acaaatacgg
    25681 ccgaaaaccc atgatgattc gggcaggtct tgctatgact atcactatgg gaggcttggc
    25741 ctttgtccca aatatctatt ggttaatctt tcttcgttta ctaaacggtg tatttgcagg
    25801 ttttgttcct aatgcaacgg cactgatagc cagtcaggtt ccaaaggaga aatcaggctc
    25861 tgccttaggt actttgtcta caggcgtagt tgcaggcact ctaactggtc cctttattgg
    25921 tggctttatc gcagaattat ttggcattcg tacagttttc ttactggttg gtagttttct
    25981 atttttagct gctattttga ctatttgctt tatcaaggaa gattttcaac cagtagccaa
    26041 ggaaaaggct attccaacaa aggaattatt tacctcggtt aaatatccct atcttttgct
    26101 caatctcttt ttaaccagtt ttgtcatcca attttcagct caatcgattg gccctatttt
    26161 ggctctttat gtacgcgact tagggcagac agagaatctt ctttttgtct ctggtttgat
    26221 tgtgtccagt atgggctttt ccagcatgat gagtgcagga gtcatgggca agctaggtga
    26281 caaggtgggc aatcatcgtc tcttggttgt cgcccagttt tattcagtca tcatctatct
    26341 cctctgtgcc aatgcctcta gcccccttca actaggactc tatcgtttcc tctttggatt
    26401 gggaaccggt gccttgattc ccggggttaa tgccctactc agcaaaatga ctcccaaagc
    26461 cggcatttcg agggtctttg ccttcaatca ggtattcttt tatctgggag gtgttgttgg
    26521 tcccatggca ggttctgcag tagcaggtca atttggctac catgctgtct tttatgcgac
    26581 gagcctttgt gttgccttta gttgtctctt taacctgatt caatttcgaa cattattaaa
    26641 agtaaaggaa atctagtgcg agtaaaaatt aatctcaaat gctcctcttg tggcagtatc
    26701 aattacctaa ccagtaaaaa ttcaaaaacc catccagaca agattgaggt tttaaagtat
    26761 tgtcccaagg aaagaaaagt aaccctacat cttgaatcta agtagatttt atggtaaaat
    26821 aatagggatt ttaaggagtt tgatatgtat aacctattat taaccatttt attagtatta
    26881 tctgttgtga ttgtgattgc aattttcatg caaccaacca aaaaccaatc cagcaatgta
    26941 tttgatgcca gttcaggtga tttgtttgaa cgcagtaaag ctcgcggttt tgaagctgta
    27001 atgcagcgtt tgacagggat tttagtcttt ttctggctag ccattgcctt agcattgacg
    27061 gtattatcaa gtagataaga aaataatggg caggactagg tctttgcctc tttttatttt
    27121 taaaggatgt ttgagaaggt tttacagtaa aagaaaatta aaaaatctag aaagaaaata
    27181 tgaaagatag aataaaagaa tatttacaag acaagggaaa ggtgactgtt aatgatttgg
    27241 ctcaggcttt gggaaaagac agttccaagg attttcgtga gttgattaaa accttgtcct
    27301 taatggaaag aaagcaccaa attcgttttg aagaagatgg tagtctgaca ttagaaatta
    27361 agaaaaaaca tgagattacc ctcaagggga tttttcatgc ccataaaaat ggctttggct
    27421 ttgttagtct ggaaggcgag gaggacgacc tttttgtagg gaaaaatgat gtcaactatg
    27481 ctattgctgg tgataccgtc gaggtagtga ttaagaaagt cgctgaccgc aataagggaa
    27541 cagcagcaga agccaaaatt attgatatcc tagaacacag tttgacaaca gttgtcgggc
    27601 aaatcgttct ggatcaggaa aaacctaagt atgctggcta tattcgttca aaaaatcaga
    27661 aaatcagtca accgatttat gttaagaaac cagccctaaa attagaagga acagaagttc
    27721 tcaaagtctt tatcgataaa tacccaagca agaaacatga tttctttgtc gcgagtgttc
    27781 tcgatgtggt gggacactca acggatgtcg gaattgatgt tcttgaggtt ttggaatcaa
    27841 tggacattgt atccgagttt ccagaagctg ttgttaagga agcagaaagt gtgcctgatg
    27901 ctccgtctca aaaggatatg gaaggtcgtc tggatctaag agatgaaatt acctttacca
    27961 ttgacggtgc ggatgccaag gacttggacg atgcagtgca tatcaaggct ctgaaaaatg
    28021 gcaatctgga gcttggggtt cacatcgcag atgtttctta ttatgtgacc gaggggtctg
    28081 cccttgacaa ggaagccctt aaccgtgcga cttctgttta cgtgacagac cgagtggtgc
    28141 caatgcttcc agaacgacta tcaaatggca tctgctctct caatccccaa gttgactgcc
    28201 tgacccagtc tgctattatg gagattgata aacatggtcg tgtggtcaac tataccatta
    28261 cacaaacagt tatcaagacc agttttcgta tgacctatag cgatgtcaat gatatcctag
    28321 ctggcgatga agaaaagaga aaaaaatatc ataaaattgt accaagtatc gaactcatgg
    28381 ccaagcttca tgaaacttta gaaaacatgc gtgtgaaacg tggagctctc aattttgata
    28441 ccaatgaagc gaagatttta gtggataaac aaggtaagcc tgttgatatc gttcttcggc
    28501 agcgtggtat tgccgagcgg atgattgagt cttttatgtt gatggctaat gaaacagttg
    28561 ccgaacattt cagcaagttg gatttgcctt ttatctatcg aattcacgag gagcctaagg
    28621 ctgaaaaggt tcagaagttt attgattatg cttcgagttt tggcttgcgc atttatggga
    28681 ctgccagtga gattagtcag gaggcacttc aagacatcat gcgtgctgtt gagggagaat
    28741 cttatgcaga tgtattgtcc atgatgcttc ttcgctctat gcagcaggct cgttattcgg
    28801 agcacaatta cggccactat ggactagctg ctgactatta tactcacttt accagtccaa
    28861 ttcgtcgtta tccagacctt cttgttcacc gtatgattcg ggattacggc cgttctaagg
    28921 aaatagcaga gcattttgaa caagtgattc cagagattgc gacccagtct tccaaccgtg
    28981 aacgtcgtgc catagaagct gagcgtgaag tcgaagccat gaaaaaggct gagtatatgg
    29041 aagaatacgt gggtgaagag tatgatgcag ttgtatcaag tattgtcaaa ttcggtctct
    29101 ttgtcgaatt gccaaacaca gttgaaggct tgattcacat cactaatcta cctgaatttt
    29161 atcatttcaa tgagcgtgat ttgactcttc gtggagaaaa atcaggtatc actttccgag
    29221 tgggtcagca gatccgtatc cgtgttgaaa gagcggataa aatgactgga gagattgatt
    29281 tttcattcgt acctagtgag tttgatgtga ttgaaaaagg cttgaaacag tctagtcgta
    29341 gtggcagagg gcgtggttca aatcgtcgtt cggataagaa ggaagacaag agaaaatcag
    29401 gacgctcaaa tgataagcgt aagcattcac aaaaagacaa gaagaaaaaa ggaaagaaac
    29461 ctttttacaa ggaagtagct aagaaaggag ccaagcatgg caaagggcga gggaaaggtc
    29521 gtcgcacaaa ataaaaaggc acgccacgac tatacaatcg tagatacgct agaggcaggg
    29581 atggtcctga ctggaactga aatcaagagt gtacgagctg ctcgaattaa tctcaaggat
    29641 ggctttgctc aagtgaaaaa tggagaagtt tggctgagca atgttcatat cgcgccttac
    29701 gaagagggca atatctggaa ccaggaacca gaacgtcgtc gtaaactcct gctccataaa
    29761 aggcaaattc aaaaattgga acaagagacc aaagggacag gaatgacctt agttcccctt
    29821 aaggtctata taaaagatgg ctacgctaag cttcttttag gacttgccaa agggaagcat
    29881 gactatgaca aacgggagtc tatcaaacgt cgtgagcaaa atcgagatat cgcgcgtgtg
    29941 atgaaagctg ttaatcagcg ataaaaagag gaattgaaaa tggaaaaatt agttgcctat
    30001 aaacgcatgc ctttgtggaa taaacaaaca atgcctgaag ctgttcagca aaagcacaat
    30061 acaaaagttg ggacttgggg gaaaattact gtcttgaagg gagctctcaa gtttattgaa
    30121 ttgacagaag aaggggaagt tctagctgaa cacctctttg aagcaggggc agacaatcca
    30181 atggcccaac ctcaagcctg gcaccgagtg gaagctgcca cagatgatgt ggaatggtac
    30241 ttggaatttt attgtaaacc tgaggattat tttgctaaaa aatacaatac caatcctgtt
    30301 cattcagagg tcctagaggc catggagaca gtgaaacaag ggaaagcttt ggatttgggt
    30361 tgtggtcagg ggcgtaattc tctttttcta gcccagcaag attttgatgt gacggctgta
    30421 gatcaaaatg gactagctct tgaaatcttg caaagcattg tggagcagga agatttggac
    30481 atgcctgttg gcctttacga tatcaattca gctagcattg aacaagaata tgattttatc
    30541 gtttcaacag ttgttctcat gtttctacaa gcggaccgca ttccagctat tattcaaaat
    30601 atgcaggaga aaaccagtgt tggtggttac aaccttatcg tttgtgccat ggacacggag
    30661 gattatcctt gctcggttaa cttcccattc acctttaaag aaggagaact ggcagactat
    30721 tacaaggatt gggaattggt taagtacaat gaaaatccag gccatttgca tcgtcgcgat
    30781 gagaatggca atcgtattca actacgcttt gcgaccttac tagctaagaa aatcaagtaa
    30841 acacacatga agattaggaa ttttcctgat cttttttctt ttttacgaat gatatagaaa
    30901 aggagggaat tcatgtttgt tgcgagagat gctaggggag aattggtaaa tgtgttagag
    30961 gataaacttg agaagcaagc atacacctgc ccagcttgtg gaggccagct ccatttgcgt
    31021 caaggaccaa gtgtacggac gcattttgcc cataaatcct taaaagactg tgattttttc
    31081 tttgaaaatg aaagtccaga acacctggcc aataaggaat ccctctatca ctggttgaaa
    31141 aaagagacaa aggttcaatt agagtacccg ctttcagaac ttaaacagat tgcggatgta
    31201 tttgtaaatg gcaatctagc tctagaagtt cagtgtagtc ccttgcctca gaaagtcctt
    31261 aaagagcgaa gtgagggcta tcgtagtcag ggttaccaag tactgtggtt gctgggtcaa
    31321 aaactgtggc tcaaggagcg tttgactcgt ctacagcaag gttttcttta tttcagtcaa
    31381 aacatgggct tttatgtttg ggaattagac aaggaaaaac aagttttaag actcaaatac
    31441 ctgatttacc aggatctccg cggtaaactc cattatcaaa tcaaggaatt ttcctatggt
    31501 caaggtagtt tattggaaat attgcgtctt ccctataaga gacaaaaaat atctcatttt
    31561 acagtttctg aggacaagga catctgtcgc tatatccggc aacaacttta ttatcaaaat
    31621 ctcttttgga tgaaagaaca agcagaagcc tatcaaaagg gagaaaatat cctgacttat
    31681 ggactgaaag aatggtatcc acaaattcga ccaatagtgg gcaaattttt ccagattgaa
    31741 caagacttga ctagctatta tcagcacttt tatacctatt accaaaaaaa tcctcaaaat
    31801 gattggcaaa agctttatcc accagccttt tatcagcaat atttcttgaa aaatatggta
    31861 gaatagaaag gatggaggaa tctaatggta ttacaaagaa atgaaataaa tgaaaaagat
    31921 acatgggatc tatcaacgat ctacccaact gaccaggctt gggaagaagc cttaaaagat
    31981 ttaacagaac aattggagac agtagcccag tatgaaggcc atctcttgga tagtgcggat
    32041 aacctactag aaatcactga attttctctt gaaatggaac gccagatgga gaagctttac
    32101 gtttatgctc atatgaagaa tgaccaggat acacgtgaag ctaagtatca agagtactat
    32161 gccaaggcca tgacactcta cagccagtta gaccaagcct tttcattcta tgatcctgaa
    32221 tttatggaga ttagcgaaaa gcagtatgct gactttttag aagctcaacc aaagctgcag
    32281 gtttatcaac actattttga caagctcttg aaaggcaagg atcacgttct ttcacaacgt
    32341 gaagaagaat tattggctgg agctggagaa atctttggtt cagcaagtga aaccttcgct
    32401 atcttggaca atgcggatat tgtgttccct tatgtcctag acgatgatgg taaagaagtt
    32461 cagctatctc atgggactta cacacgtttg atggagtcta aaaaacgtga ggttcgccgt
    32521 ggtgcctatc aagctcttta tgcgacttac gaacaattcc aacacaccta tgccaaaacc
    32581 ttgcaaacca atgttaaggt gcaaaattac cgtgctaaag ttcgtaacta caagagtgct
    32641 cgtcatgcag ccctcgcagc gaattttgtt ccagaaagtg tttatgacaa tttggtagca
    32701 gcagttcgca agcatttgcc actcttacat cgctatcttg agcttcgttc aaaaatcttg
    32761 gggatttcag atctcaagat gtacgatgtc tacacaccgc tttcatctgt tgaatacagt
    32821 tttacctacc aagaagcctt gaaaaaagca gaagatgctt tggtagtctt gggtgaggat
    32881 tacttgagcc gtgttaaacg tgccttcagc gagcgttgga ttgatgttta cgaaaatcaa
    32941 ggcaagcgtt caggtgccta ctctggtggt tcttacgata ccaatgcctt tatgcttctc
    33001 aactggcagg acaatctgga caatctcttt actcttgttc atgaaacagg tcacagtatg
    33061 cattcaagct atactcgtga aactcagcct tatgtttacg gagattactc tatctttttg
    33121 gctgagattg cctcaactac caatgaaaat atcttgacgg agaaattatt ggaagaagtg
    33181 gaagacgacg caacacgctt tgctattctc aataacttcc tagatggttt ccgtggaaca
    33241 gttttccgcc aaactcaatt tgctgagttt gaacacgcca ttcaccaagc agatcaaaat
    33301 ggggaggtct tgacaagcga tttcctaaat aaactctacg cagacttgaa ccaagagtat
    33361 tatggtttga gtaaggaaga caatcctgaa atccaatacg agtgggctcg tattccacac
    33421 ttatactata actactatgt atatcaatat tcaactggat ttgcggccgc ctcagccttg
    33481 gctgaaaaaa ttgtccatgg tagtcaagaa gaccgtgacc gctatatcga ctacctcaag
    33541 gcaggtaagt cggactatcc acttaatgtc atgagaaaag ctggtgttga tatggagaag
    33601 gaagactacc tcaacgatgc ctttgcagtc tttgaacgcc gtttaaatga gtttgaagcc
    33661 cttgttgaaa aattaggatt ggcataaaat ggttgaatcg tatagtaaga atgctaacca
    33721 taacatgcgt cgtcctgtcg tcaaagaaga aattgtagac ttgatgcgtc agcgtcaaaa
    33781 gcaagtcaca ggttccttga aagaattgga agactttgcc cgcaaggaaa atattcctat
    33841 tattccccat gaaacggttg cttatttccg ttttcttatg gaaaccatgc agcctaaaaa
    33901 tattctggaa attgggacgg ctatcggttt ttcagctctc ttgatggctg aacatgcgcc
    33961 aaatgctaag attacaacta ttgatcgtaa tccagaaatg attggttttg ccaaggaaaa
    34021 ttttgcccag tttgacagtc gcaagcaaat cactctccta gagggagatg cggtggatgt
    34081 cttatctaca ctgacagagt cttatgattt cgtctttatg gattctgcca agtctaaata
    34141 catcgtcttt ctgccagaaa tcctcaaaca tttggaagtt ggtggtgtgg ttgtcttgga
    34201 tgatattttt caaggtggtg atgttgccaa ggatattatg gaagtccgtc gtggtcagcg
    34261 aaccatttat cgaggccttc aaaaattatt tgatgcaacc ttagacaatc cagaactcac
    34321 cgcaacatta gtgcctttag gagatggtat tctcatgctt cgtaaaaatg tagcagatgt
    34381 tcaactgtct gaaagcgaat gattttcaga aaaatttaag aaaaaatagt aaaatagata
    34441 gagtaacact tatctcaaag gagtagacat gaagaaaaaa ttattggcag gtgccatcac
    34501 actattatca gtagcaactt tagcagcttg ttcgaaaggg tcagaaggag cagaccttat
    34561 cagcatgaaa ggggatgtca tcacagaaca tcaattttat gagcaagtga aaaacaaccc
    34621 ttcagcccaa caagtcttgt taaatatgac catccaaaaa gtttttgaaa aacaatatgg
    34681 ctcagagctt gatgataaag aggttgatga tactattgcc gaagaaaaaa aacaatatgg
    34741 cgaaaactac caacgtgtct tgtcacaagc aggtatgact cttgaaacac gtaaagctca
    34801 aattcgtaca agtaaattag ttgagttggc agttaagaag gtagcagaag ctgaattgac
    34861 agatgaagcc tataagaaag cctttgatga gtacactcca gatgtaacgg ctcaaatcat
    34921 ccgtcttaat aatgaagata aggccaaaga agttctcgaa aaagccaagg cagaaggtgc
    34981 tgattttgct caattagcca aagataattc aactgatgaa aaaacaaaag aaaatggtgg
    35041 agaaattacc tttgattctg cttcaacaga agtacctgag caagtcaaaa aagccgcttt
    35101 cgctttagat gtggatggtg tttctgatgt gattacagca actggcacac aagcctacag
    35161 tagccaatat tacattgtaa aactcactaa gaaaacagaa aaatcatcta atattgatga
    35221 ctacaaagaa aaattaaaaa ctgttatctt gactcaaaaa caaaatgatt caacatttgt
    35281 tcaaagcatt atcggaaaag aattgcaagc agccaatatc aaggttaagg accaagcctt
    35341 ccaaaatatc tttacccaat atatcggtgg tggagattca agctcaagca gtagtacatc
    35401 aaacgaatag tccaaatcaa tgagtcaggg aaaaaactcg acttcaggaa aaaatgaagc
    35461 aaacattccc acaataaaac gcatagtaca aggtttgtac tgccccccca aaagttagac
    35521 aattaattta tccgaaggat ttagttctgt attgcacaga gctaagtcct tttagtttta
    35581 tcttaattct cttgttgttg taataatcaa tatagtctat aatggcttgt tccaattgat
    35641 taagtgattt aaatgttttc tcatagccat aaaacatttc ggattttaaa ataccaaaga
    35701 aagattccat cctaccgttg tcttggctgt ttccctttcg tgacatagat gcttgaattc
    35761 ccttactctc taggaagcga tgataagaat cgtgttggta ttgccagcct tggtcactat
    35821 ggagaatcgt attctcatag tgcttctctt tgaatgcctg ttccaacatt aacgatcaat
    35881 caatttaatc atgtacctaa gattagaatt gtttatctca aatttatttg aaag
//
LOCUS       DAVKJU010000017        34994 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVKJU010000017 DAVKJU010000000
VERSION     DAVKJU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34994
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.017"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(321..885)
                     /locus_tag="VW191_000742"
                     /pseudo
     CDS             complement(321..885)
                     /locus_tag="VW191_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(961..1173)
                     /locus_tag="VW191_000743"
     CDS             complement(961..1173)
                     /locus_tag="VW191_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9623535.1"
                     /translation="MIQPLLHADETSYRVLENDSHLTYYWTFLSGKAENQAITLYHHD
                     QRRSGSVVQEFLGDYSGYVHCDMLRQ"
     gene            complement(1182..1487)
                     /locus_tag="VW191_000744"
     CDS             complement(1182..1487)
                     /locus_tag="VW191_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HEU9623536.1"
                     /translation="MFPVETEEITYKRKKSKGKRQAILAQFDSEEVHHQVEESICPDC
                     QDDLKEIGASLQRQELVFIPAQLKRVDHIQHAYKCQTCSKNNPSDKIVKAPIPKAPL"
     gene            complement(1474..1668)
                     /locus_tag="VW191_000745"
     CDS             complement(1474..1668)
                     /locus_tag="VW191_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9623537.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(1838..2188)
                     /gene="tnpB"
                     /locus_tag="VW191_000746"
     CDS             complement(1838..2188)
                     /gene="tnpB"
                     /locus_tag="VW191_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEU9623538.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(2169..2354)
                     /locus_tag="VW191_000747"
     CDS             complement(2169..2354)
                     /locus_tag="VW191_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623539.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2658..4220)
                     /gene="guaA"
                     /locus_tag="VW191_000748"
     CDS             complement(2658..4220)
                     /gene="guaA"
                     /locus_tag="VW191_000748"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU9623540.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4362..5060
                     /locus_tag="VW191_000749"
     CDS             4362..5060
                     /locus_tag="VW191_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9623541.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5103..5999)
                     /locus_tag="VW191_000750"
     CDS             complement(5103..5999)
                     /locus_tag="VW191_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU9623542.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGTLMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLIIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYTIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(6008..6943)
                     /locus_tag="VW191_000751"
     CDS             complement(6008..6943)
                     /locus_tag="VW191_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEU9623543.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEY
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6940..7665)
                     /locus_tag="VW191_000752"
     CDS             complement(6940..7665)
                     /locus_tag="VW191_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEU9623544.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7749..8384)
                     /locus_tag="VW191_000753"
     CDS             complement(7749..8384)
                     /locus_tag="VW191_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEU9623545.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8386..9213)
                     /locus_tag="VW191_000754"
     CDS             complement(8386..9213)
                     /locus_tag="VW191_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9623546.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKVRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9235..9375)
                     /locus_tag="VW191_000755"
     CDS             complement(9235..9375)
                     /locus_tag="VW191_000755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623547.1"
                     /translation="MYHYQTYILEDFIEDGSHCDQLDEATKERIDTAYKEMNEHVGLK
                     WY"
     gene            complement(9571..10287)
                     /locus_tag="VW191_000756"
     CDS             complement(9571..10287)
                     /locus_tag="VW191_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU9623548.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGNKKLIIY"
     gene            10810..11421
                     /locus_tag="VW191_000757"
     CDS             10810..11421
                     /locus_tag="VW191_000757"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU9623549.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11466..12194
                     /gene="rlmB"
                     /locus_tag="VW191_000758"
     CDS             11466..12194
                     /gene="rlmB"
                     /locus_tag="VW191_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU9623550.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKNLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENSLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12233..13144)
                     /gene="trxB"
                     /locus_tag="VW191_000759"
     CDS             complement(12233..13144)
                     /gene="trxB"
                     /locus_tag="VW191_000759"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU9623551.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVRKIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13210..13434)
                     /locus_tag="VW191_000760"
     CDS             complement(13210..13434)
                     /locus_tag="VW191_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEU9623552.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13512..14255)
                     /locus_tag="VW191_000761"
     CDS             complement(13512..14255)
                     /locus_tag="VW191_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9623553.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14255..15055)
                     /locus_tag="VW191_000762"
     CDS             complement(14255..15055)
                     /locus_tag="VW191_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9623554.1"
                     /translation="MNVTTILASDWYQNLMQLIPNGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15213..15569)
                     /locus_tag="VW191_000763"
     CDS             complement(15213..15569)
                     /locus_tag="VW191_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEU9623555.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15563..16093)
                     /locus_tag="VW191_000764"
     CDS             complement(15563..16093)
                     /locus_tag="VW191_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU9623556.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(16093..16587)
                     /locus_tag="VW191_000765"
     CDS             complement(16093..16587)
                     /locus_tag="VW191_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623557.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16722..17168
                     /locus_tag="VW191_000766"
     CDS             16722..17168
                     /locus_tag="VW191_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEU9623558.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            17165..17812
                     /locus_tag="VW191_000767"
     CDS             17165..17812
                     /locus_tag="VW191_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEU9623559.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILTASVLQYIAIVYYM"
     gene            complement(17833..18414)
                     /gene="pdxT"
                     /locus_tag="VW191_000768"
     CDS             complement(17833..18414)
                     /gene="pdxT"
                     /locus_tag="VW191_000768"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEU9623560.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18415..19290)
                     /gene="pdxS"
                     /locus_tag="VW191_000769"
     CDS             complement(18415..19290)
                     /gene="pdxS"
                     /locus_tag="VW191_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEU9623561.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19442..20821)
                     /locus_tag="VW191_000770"
     CDS             complement(19442..20821)
                     /locus_tag="VW191_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU9623562.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALAGGKIELFRNGAF
                     LVDKKQETSIPDVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDFQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            21115..22038
                     /locus_tag="VW191_000771"
     CDS             21115..22038
                     /locus_tag="VW191_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEU9623563.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            22098..22703
                     /locus_tag="VW191_000772"
     CDS             22098..22703
                     /locus_tag="VW191_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9623564.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFTDKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22722..23966
                     /locus_tag="VW191_000773"
     CDS             22722..23966
                     /locus_tag="VW191_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU9623565.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRYFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AFTGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(24189..24446)
                     /locus_tag="VW191_000774"
     CDS             complement(24189..24446)
                     /locus_tag="VW191_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEU9623566.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24488..26524)
                     /locus_tag="VW191_000775"
     CDS             complement(24488..26524)
                     /locus_tag="VW191_000775"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU9623567.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26736..27653)
                     /gene="glyQ"
                     /locus_tag="VW191_000776"
     CDS             complement(26736..27653)
                     /gene="glyQ"
                     /locus_tag="VW191_000776"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU9623568.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27846..28607)
                     /locus_tag="VW191_000777"
     CDS             complement(27846..28607)
                     /locus_tag="VW191_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9623569.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(28880..29722)
                     /locus_tag="VW191_000778"
     CDS             complement(28880..29722)
                     /locus_tag="VW191_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU9623570.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERENLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29836..31227)
                     /locus_tag="VW191_000779"
     CDS             complement(29836..31227)
                     /locus_tag="VW191_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU9623571.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31462..31839)
                     /locus_tag="VW191_000780"
     CDS             complement(31462..31839)
                     /locus_tag="VW191_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9623572.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            32054..33031
                     /locus_tag="VW191_000781"
     CDS             32054..33031
                     /locus_tag="VW191_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU9623573.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPVVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            33028..34110
                     /locus_tag="VW191_000782"
     CDS             33028..34110
                     /locus_tag="VW191_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9623574.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(34174..34994)
                     /locus_tag="VW191_000783"
                     /pseudo
     CDS             complement(34174..34994)
                     /locus_tag="VW191_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10367 a         7796 c         6296 g        10535 t
ORIGIN      
        1 tcatataata aattgtaacc gcccaataac gaagtctatt gaaaaatctc cagattagtg
       61 aactcacgga tagttcctaa tctggagatt tcttatttgc acttttcttg tacaacttta
      121 gtccacggta aataggcctc taaaacctct ttgtttacga gagtttccac gtttggaaga
      181 cattctagaa gataggatag atatttctca ctatttataa tggattgaaa taagatatga
      241 acaaatcgat taggaaagga aaattgattt atagaaatgt tttagcagtc gatgcgtact
      301 gttatagatt caacgcacta taattgatga cgtttagctg tttccaacaa actcataata
      361 atagctcttg ctttagctcc ttcaaaactt tgagaaaata accaccctcg cttttttaaa
      421 ctgagcttag gctaaaagag tccactggac tcttttactc cgtcccataa ccaatgattt
      481 aatggcgcat tcagctagat tattggaaag gaccagatgt ccgtctttca aaatagtctt
      541 aaaggtttct ttatacttga ggctatactt gaggctgtat tcaattgccc ttcctagttt
      601 tgaacccgat aaaactgact gacgccggca ccaagcaaag aaatcttcca ttaggggttg
      661 gagctcttct tgacgtttct gtagtcgttc atcagctggc aaagcctccc agtttctttc
      721 caaggaaaat aactgattac aataagctaa acctttagct cctaaggatg atttattcgc
      781 ttgcttgggg gtcgcttcaa aaaacttcct tctcacatgt acccaacatc caacaagttc
      841 agcttcttcc agttgacaat aagcttctag cggttcgcag tttaccaagc ttagcgtcgc
      901 cttgctccta aaccttggac tgctatcgca taggcagaac tagaggacta aagtcctaag
      961 ttactgccgc aacatatcac aatgcacata gccagaataa tctcctagga attcttgcac
     1021 taccgaacca ctccgacgct gatcatggtg gtacagcgtg attgcttgat tctcagcttt
     1081 cccagacaaa aaggtccaat agtaggtcag atggctatca ttctctagaa cccgatagga
     1141 ggtttcatct gcatgaagta agggctgaat caatagtctc gttataaagg ggctttagga
     1201 ataggagctt ttacaatttt atcacttgga ttattcttac tgcatgtttg gcatttgtaa
     1261 gcgtgttgga tatgatccac tctttttaat tgcgcaggaa taaagaccaa ttcttgtcgt
     1321 tgaaggctcg ctccaatctc ttttaaatcg tcctgacaat cagggcaaat gctctcttct
     1381 acttgatgat gaacttcttc tgaatcaaat tgggcaagaa tagcttgacg tttcccttta
     1441 gatttcttac gtttgtaggt aatttcctct gtttcaactg ggaacatctc catctttctc
     1501 agatggagac tcttcctcaa aaaggttgag ttgtccaaaa gggcaaacac ttttctcaga
     1561 ggattttcca gagagctttt gcgttagata agccacttgt tcacacagaa gggaaagttc
     1621 attagtgaga ctatcaatta tagcactctg ttgttgaatg attttcaatc ttctcccata
     1681 gactttcctc cttatttcct aactttatta tatagtgtat tgaatctata acagtacacc
     1741 ttgactgcta aaatatttct ataaattaat ttgactttcc tgatagagat gttcatatct
     1801 tatttcaatt cactatacta aaaagaaagt cctcatttca atagaaatca cgactttctg
     1861 ataaatttat tttgggagtg atagaaaagc ccttcataag ccaatctact tgttcaggtg
     1921 tgagagcttt gacatccttt tctgtactgg gccaagtcag tctgccgttc tcaaagcgtt
     1981 tatatagtag ccaaaatcct tgaccatccc agtaaagggc tttaaagcgg tctttacgtc
     2041 caccacaaaa gagaaaaact tgaccggaga aaggatccaa ttcaaagtgg gttttaacca
     2101 gataagccag tgaatcgatt ccttgtctca tatcagtctt cccacacacg agatagacct
     2161 gccctaggct agatagatga attgtcatag agcaacacct tatccaaaag ctgttctacc
     2221 atttcgagat tgagagattg aaaaaaactt acttcaagct tgccaatacg aattttaagc
     2281 agaatatcat ttctcttttt agaatcaaaa ggacgagatt gtggaatctc tacaggaaca
     2341 ataggttgtg gcattaaaat atcctccaat agttttactt tactaatagt atacaggagg
     2401 aactccatga atgatagata ccttgttatg acgcgcttac tatattagaa agttggctct
     2461 ctaattaaat taattctcat tatatatatc aaaagaccgc agtttgaaaa ctacggtcta
     2521 attttttgtc caaatgtaaa tcacttggaa ctattattca atttattctt tttagtaaat
     2581 gttcccgttt taatcccagt tacagttttt aatcctctgt aaccgttgat tttacaaagc
     2641 ttttaatttt tttgcgatta ctcccactca actgttgcag gtggtttact tgtaatatcg
     2701 tagacgatac ggttaacatg atccacttca tttacgatac gtactgagat tttttgaagt
     2761 acttcccatg gaattttggc aaaatcagca gtcataccat cgatagaagt gatagcacgg
     2821 attgcaatcg tgtagtcata cgtacgaccg tcacccataa caccgactga acgaacgcct
     2881 gtgttaacag tgaagtattg ccaaatatcg cggtcaagtc cagctttagc gatttcttca
     2941 cgaagaatag cgtctgattc acgaacggtt tcaagtttct cttcagtgat ttcacccatg
     3001 acacggatag caagtcctgg tcctgggaat ggttggcgcc atacgatatg gtctggcata
     3061 ccaagctctg taccaagagc acgaacttca tccttgtaaa gagtattgag tggttcaatc
     3121 aattcaaact gcatatcttc tggaagacca cccacgttgt ggtgtgactt gatagtttga
     3181 gctgtatccg taccagactc gataacatct gtatataaag taccttgagc aaggaatttc
     3241 acatctttga gcttgcttgc ttcgtcatcg aatacataga caaactcgtt accgatgatt
     3301 ttacgttttt gttcaggatc agaaacgcca gcaagtttgt caaggaaacg tttagcagcg
     3361 tctgctttga cgatattcaa accaaactta ccaccgagca tgtccataac ttgatcagct
     3421 tcgcctttac gaagaagacc gtggtctacg aagatacaga tcaattgatc gccaatcgct
     3481 ttttggagaa gaaccccaac gacagatgag tcaacaccac ctgatagacc aagaaggaca
     3541 cgtttatcac cgacggtttc acgaattttt ttgatctgca tgtcaatgaa attatccatt
     3601 gaccagtcac ctttagcctt acaaatgtta agggcaaagt tacgaaggat atcatttccg
     3661 tatacagaat gacgaacttc tgggtggaat tggataccgt aaatgtgttt atctgggttt
     3721 tcgatggctg cgtatgggca gtcagctgat gtacctgtac gaacaaagtc agcaggaatc
     3781 tcagtaaccg catcaccatg gctcatcaaa acagtctgtt catcaggtgt tgattcaaaa
     3841 agcgctgatg gtgtgtgagt tagggttgat tgaccgtatt cacgatttcc agcatcacct
     3901 gcaggaacaa cttttcctcc aagtttatgg gtcaataact gcataccata acagattccc
     3961 aaaattggaa ttccgagttc gaagatttct gggtcaatat caaatgaacc atcttcatat
     4021 acagaatttg gaccacctga tagaataatt cctacaggat tgacttcacg aacttcagca
     4081 gctgaaattt tatggctttt tagttctgaa aaaacaccaa tctcacggat acggcgtgaa
     4141 atcagctggt tgtactggct accatagtcc aatacgatga ttttttctac atcttgcaaa
     4201 tcagttgaaa tgttgctcat ctttttcctt tctcaaaaga aatatctttt cccaatattc
     4261 tatcacaaat aagggcgaat taccaactaa acaaggcgaa gtttgacttt ttctagttta
     4321 ttggggtaca atagagaaaa gatagaaatg aagtgaaaaa tatgctacct gcttatatga
     4381 aaatccatga tcagataaaa aaggatattg acgagcaccg ttgggctatt ggtgagaggc
     4441 ttcccagtga aagagattta gctgagcagt ttgcggtcag tcgcatgacc ctccgccaag
     4501 ccgtatctct attagtcgaa gaaggcgtct tagagcgccg tgtaggaagc ggcacctttg
     4561 tttccagtac tcgagtacaa gaaaagatgc gagggacaac cagttttact gaaattgtca
     4621 aatcccaagg taaagttccc tctagccagc tcatttccta caaaaaaacc attcccaatg
     4681 agcaagaagt tgccaagcta ggaatttttc caacggacaa tattatccga atggagcggg
     4741 tccgctatgc cgaccaagtt cccctagttt atgaagttgc ttctattcct gaaaaattca
     4801 ttaaggactt taaaaaagaa gaaatcacca gtcatttctt ccaaaccttg caaaaacatg
     4861 gctatcgtat cggcaaatct caacagacca tctatgctcg ccttgctaaa gaaaagattg
     4921 cccactattt ggaagttgaa aaaggacatg ctattcttgg attgacccag gtttcctacc
     4981 tagaagatgg tactgctttt gaatacgtaa aaagtcaata tgtaggcgaa cgctttgaat
     5041 tttatcttga aaataattag attaccacgc aaaaactctc tgtcacggac aaagagtctt
     5101 ttttattttt ctaagaataa atctttcaaa gaaatcaaag ttacagcaaa caatatcatt
     5161 gccataccaa gaaaatcaat ggtataaaat tgttcattca ttattaagaa ggcaaagaaa
     5221 accgccgata ttggctcaat tgaagccaac aagcttgact tgaccggtcc tatcagactg
     5281 gctcctttaa ggaaagctgt ataggcaaag acagtcccga taaggataat gcctgcaaac
     5341 gcaaggagaa aatcaagact agtcgggata tcggcctgta gaacccctgt aaaaggaagg
     5401 gcgaccaaac ctgctatgac cattcccaca ccaatgatca agctgctccc ccacttttta
     5461 atcaaggcta tgggtaaaat gatatacaga gcataagtca aggcagaaaa gagaccccag
     5521 aacaggccag caggtgtcat ggataactgg tccaactgcc catgtgttgc gatcaggaag
     5581 gttcctccga tggcgaatat gatggaaact atctctccca gtgtcggtgc caccctatcc
     5641 ttgatacagc tataaattaa aattccgaca ggacaaacat actgaagcac cgtcgctgtt
     5701 cccgcattgg tctcctgaat agcagacaga taggcgaatt ggttgagaaa aagaccaatc
     5761 agagcaaaaa taagaagaga cagcaaactc tttctatcct ttaaaaagac cagtatttta
     5821 tcctttgcag tagcataagc caagagcatg agagttccac cagcgattaa aagacgcaag
     5881 ttggtcaaga ccagagccga aattccgtgt gccatcaggt attggccact cgttcctgac
     5941 aacccccaag caataccagc cacaactgtt agtaaagtcc cttttaaact atttgacatg
     6001 tctctcctta ctcttctttg cgaatcaagt tcatcacttg attgcgatct aatatccatt
     6061 gagctctctc gtccttttca taggtacgat tcgataggaa aatagtggct tcttgcttct
     6121 gacgattcca catgataaag gtacctgtat agcccgtatg gtctagccaa tctccttcca
     6181 aattccatgc taaagaacgt tccttgtcat ccaaaggaga aaaattttga tttaagtctc
     6241 ttgcaaaatc atctgctaaa tagtgttcta aaaagatttg taaatccttt atagtcgaaa
     6301 ataaaccagc actcccagca tgtctaccca ggagacgagc cttgggatca tgcactatgc
     6361 ctgcctctac acctctaact gttggaacag caagctcaac tggcccaaac ttagtttccg
     6421 tcattcccca aggtttccag acttgatcct ttaaaatcac atccaaatct tgattaaaaa
     6481 ttctttccaa aataaagccc aacagcaaaa aatggacatc cgaataaaga aaggctggct
     6541 gacttcgtct gttgagatga aacatcgctt cctttaattc aggggctgtt aaaagatcac
     6601 gattaggaat aaaaggatca aggtctgttg catgagtcaa gagctggcga atagtgatgt
     6661 ctggataatc actctcaggt aaaaaatcta ttaccagtct atcaatatct aattgaccta
     6721 tttcccacaa gaaggtacaa actgtgccaa ccccaacaac cttgctgaca ctagctaggt
     6781 cataaactag tcctgcctca gtctgcaagc catactctgg gtcactctgg cctaaataga
     6841 actctgtcca ttgattgtcc ttaaaatacg caaaagaggc tccgggataa attcctgcct
     6901 cgatttgttc ttctattttt ttaataatct tggtccactt catacttctt caaaccacaa
     6961 ttcaacatta tttctatctt taccaaggaa gaatttttca gacttaggaa taaaatattc
     7021 agtagactca aacttctggc gaagacttgc tatgtctaat tcattgacca agaacttgag
     7081 catagataag tcccaggtaa ccgtattgtc cacagtcaaa tcctgcccct gagctggaat
     7141 aaaatcaagg gatgccccaa tttcagatga ttccaagaaa ctttcgatat cagttgggag
     7201 atgtaattcc atagaaatct caaatttact taaagaaatt gatgccaaat ctgtttgaaa
     7261 ttcaggcttt tctcctactt ctactagact tgctctgtca tcttccgcat gaatcaaaat
     7321 caaatcatct tctggtgaga aaatttcaaa agcgtagcca ttttgacctt tatataatcg
     7381 atgaatcgaa tctgttttag ataagattcc ttcaatttct aagggatttt ccaccttgac
     7441 aatcaatcta gctagttttt ttcttccctc taccttacga gtacgcatac tgggagcttc
     7501 ttctaaaacc agcttttcaa gacccgtttg gtcacctagt gacagaaagg ccgattcttc
     7561 taacaaggcc ttcattccaa gggtttcaat ataaaatgtt tcatttaatt ttctattatt
     7621 agcttttaaa gtaggaataa tccgtacaat ctgatttaca ttcataaatt cctccgtcac
     7681 tttttatttt aaaggatttt agaatttttt acaagatatt atgctcaaat ttaacattat
     7741 ttagctattt agtaaagata gtctttcaag gattttgaca aagcctgata accagcaata
     7801 ctgaggtgca gtccatcagt tgtatattct tttttgagtt ggcctgcttg gtctgtcaaa
     7861 caatcaaata ctggcacaaa ttccacctgc atataggcag atgcaagctc ttgataggct
     7921 tgattccagt tctgaatttt ttcattcgag cggatataga ctgcctgctg gtactcctct
     7981 ccctcattga caggcaaaat ggaaagcaat ttaatctctg tcaatggata atcgcgagca
     8041 acggattgaa tgatagcttc gagattattg agagcctcat tcacaggaac atcctttccg
     8101 atatcatttg tcccaatcag aagaaaaatt ttatctactg ctccaccata tagatgagca
     8161 tcaagattct ctagtaacag tcctgtctga tagccacgaa ttcctcgatt gacaatcgtc
     8221 tttgaagtcc caaatagctc ctgtagagga taatactcga caatggaatc cccaataaaa
     8281 agaatgtttg gttctacaac agaaatgtga tttaggtgac gatacttagt ttgaattttt
     8341 tcttgttcct ttaggagcca attttctaat aactgtactg ccacttcatt ctcctttttc
     8401 taaccagttt tccaagactt gataaactcc tccttggctg ttggctggtg ctagagcagt
     8461 cgccacagct ttggctttct catcagcatt ttccatcgca taggcaattc cagccatttc
     8521 aagcatttca acatcatttt cactatcacc aaaagccatg atttcttggg atttcaagtc
     8581 ccagcgcttg agtaattcct ccaagcccca tgctttatga atcccagctt ggaggatatc
     8641 aatgcaacca tagccactgg atacagctcg gacacggcca tcaaagagag cattgatttc
     8701 ttccaaaacc gaactcaaac gttcctcacc aacaaccatg ctcatcttga gcacaccacc
     8761 aaagaggtca gatgttaatt catccacaaa ttgcatccgt tggtagaatt tttcaatcat
     8821 ttctggagtc ataaaacttt caagatctgt aaaaatcgta ccttccttga caaaatcacc
     8881 cttcatcccc gttacaacaa actggtcctg acacgctcga accttgaaat gagtcaaagc
     8941 cttgttgaca atggcgtcat cccatgtctg agcctgaatc aattcattgt tttcaaaaat
     9001 acgagcgcca ttagcaacaa ccagaaccac tcgatccacc aagggactca gtagttgtct
     9061 catgcggtga atttcattgc ccgtcgcaat gacaaaacga atgccccttt gatccaactg
     9121 atctaaaatc ttttccaatc gtgggagatc aagctgacct ctagcatcca gcaaggtccc
     9181 atccatatct gtcgcaatta ccttaatcat catttacttt cctctggatt caagctaata
     9241 ccacttcaat cccacatgtt cattcatttc tttataggct gtatcaattc gttccttagt
     9301 cgcttcatct aactggtcac aatgactgcc atcctctata aaatcttcca aaatataagt
     9361 ttggtaatga tacaagggat atccttctgg tgaaaaggtt ccctttggtg ttcgattgac
     9421 ctaagtagga tgagctttag ctgttccgat agttgttttt ccatccttct tcttgatggt
     9481 gacatccatg agaacaccac gttcagtcca cttagcattc tcttcatctc ccatagattc
     9541 aatccgttga ttggaaatga agttccccat ttaatagata atgagtttct tatttccatc
     9601 tttttcaacc gtttcagatg gttcaacaac gtgaggatgc cctccaaaga taatatccgc
     9661 tccccaatcg atcatcttgt gataaagagc tttttgttct tcagttggtt ccaatcgata
     9721 ctcaacaccc atctgaggca tgataatggt gatatctgct tccttctctg cccgttcaac
     9781 ttcagccttt atcttatctt cgtttaaatc tgaaagataa cgattatagt cttcctgaaa
     9841 aatatactgc tcaattccat tgaaaccata ggaataggct aacaatgcaa ccttgatagc
     9901 attcacttcc ttaatgacca gcggagcctg atcacgtggt tcgtgcgtat aaactccgat
     9961 tggagtgatt ccagctttct caataatatc ggccattgaa ataactccct caatttgcga
    10021 atccaaaata tgattatgag ctaaatccag cacatgataa cctgcttcct taatagcatc
    10081 cataacttca acaggagcat taaagagaag ataacccgct aaataatgat ccttattaat
    10141 ggttccttca aaatcaccaa tagctaaatc tgcttgcttg agccaaggag tcacatactc
    10201 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
    10261 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
    10321 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
    10381 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
    10441 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
    10501 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcattc
    10561 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
    10621 caattgaatt gaacttccca tctattttct ataaatccta aatgccataa tgctttcaat
    10681 tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga aatgaactaa tccacagtgg
    10741 cttattccaa gtataccact tgggctttgg cagtagttaa ctgcactaaa cataatataa
    10801 ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac
    10861 gatattatcc gtgaagggaa tcctactcta cgcgcgattg ctgaggaagt cactttcccc
    10921 ctatctgacc aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa
    10981 gatcctgtca tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag
    11041 ttagatatct caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa
    11101 actccacagg aagcctacga tttggaaggc attatgtaca atccaaaaat cgtctctcac
    11161 tctgttcaag atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg
    11221 cctggctatg ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa
    11281 aaacaccgta tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac
    11341 attaacggta tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat
    11401 ggtttactga ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata
    11461 gaggcatgaa aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gccctccttg
    11521 caaatacagg aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag
    11581 tcaaggaact agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaaaatctct
    11641 ctgagatgac tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct
    11701 atagcgagct agattacatc cttgcaaaaa cacgccaaga agaaaattca cttctattga
    11761 ttctagatgg tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga
    11821 ccaatgtttc aggtgtcatc attcccaagc accgtgctgt cggagtaact cctgtcgttg
    11881 ccaaaacagc cacaggtgct attgaacacg ttccaattgc ccgagtaacc aacctcagtc
    11941 aaaccttaga taaactcaag gatgaaggtt tctggacctt tggaacggat atgaacggta
    12001 ctccttgcca caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa
    12061 aaggtatctc tagcaacata aaaaaacagg tcgatgaaat gattaccatt ccgatgaatg
    12121 gacatgttca aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc
    12181 gaaatagact ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat
    12241 gttctgtaat gaatttatag gcttcttgac cagcgatagc tccatctcca actgctgttg
    12301 ttacttggcg aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag
    12361 tcttcatgtg gctatctgtc acaatccagc ctgcctgatc ttggatattc aattctttaa
    12421 caaaatcgct aagagggtcc aaaccaacat agataaagac accaccgaag gcttgctctg
    12481 tcacttgacc tgttttcaca ttttcaaaaa cgacagattc tactctgttt tcacccttga
    12541 ttttccttac tacagaatcc catataaagc tgattttctc attggcaaag gcgcgatctt
    12601 gtaaaacctt ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa
    12661 aacgagtcaa gaagagggct tcttcaacag ctgaatctcc accaccaact accaataaat
    12721 cttggtcacg gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca
    12781 gttcttcttc tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg
    12841 tacgtgtttc atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga
    12901 cattttcaac ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct
    12961 tttcagccaa ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg
    13021 tattattcat ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc
    13081 gagcagcata caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt
    13141 acatatagat tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc
    13201 acgcaaggat tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg
    13261 gacaaccaag actccaatca taatcatatc atagagatgg gcttggcgag ccttggctgt
    13321 cttatcaact cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag
    13381 gatggtggtc aagatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc
    13441 tctcatttct atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt
    13501 tatctattat atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct
    13561 tctttagggg tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct
    13621 aagaagagaa tcttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc
    13681 atggtctgac ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct
    13741 gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca
    13801 cgcgctaggg caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca
    13861 cggtccgaaa gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca
    13921 gataatttct tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca
    13981 aacaaattaa actgttggaa aaccatagac aacttacggc gtagggcaag gatttcttct
    14041 tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt
    14101 gtttcaagat aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc
    14161 aaggctacaa cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct
    14221 gaaaaggatt tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg
    14281 tttgcactgt atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgagacttt
    14341 caattccgat attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc
    14401 gataatcagc tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa
    14461 aagctagaga tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta
    14521 ccaccgctgc attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac
    14581 tgcgtgccgc ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac
    14641 tagcataagc tgcctcatta aaggcaaagg cgacaatcgc aaaagctgca gctggaatcg
    14701 cattgatatt gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc
    14761 cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga
    14821 aggcctgcaa gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg
    14881 ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt
    14941 ggacaattct agggattcca tcaaagaccg aacgtaggct aaacagcttg ccattcggaa
    15001 tcaattgcat caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa
    15061 catcctcttc tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct
    15121 acggtcagat acagactttg tctacctact agtttatcat actttgaaac agggaagtta
    15181 tatattttat ctatcaaaga gatagataaa aactatttca gtcccagttc ttcataagat
    15241 tttcgataac cgatttgctt aacggttcca ttttctacta aaatgggccg ttttaacaac
    15301 ataccgtcac ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct
    15361 tttagcccta attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat
    15421 cctgaggttt ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg
    15481 acggctttat agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa
    15541 catttggggt attcgataaa ttctaacatg tgcttctcct tctattgctt ctatctttaa
    15601 aatctactcc ttcatgctcc aagagccaag ctttcttttc cactcctgca gcataacctg
    15661 tcagacgctt gcctgctccc aacacacgat gacaaggtac taggatagac caaggattgc
    15721 gtcccactgc tccaccaatt gcttgagcag aagccacttg caggtcttga gcaatttgtc
    15781 cataggtcac tgtctgacca taaggaatgc cctgtaaata gtcccaaact ctcttttcaa
    15841 aattcgttcc gattggcgcc aagagcaagt tggataaatc ctgaggcttg cctttaaagt
    15901 aatcatctaa gcaagcaata actgggtcta aaataggatg actaacaact tcttctatcg
    15961 tttcatctcc tagtcccctc tcaaaatgct tctgctcctg aacccaaatt ccatacaaat
    16021 aatggtcatc agctacaagt gatagaatac caattggtga ggagtagagt atttttacat
    16081 acttcttttg cattatttct ttaatttttg ataggccaag cgttcgccat ctactggcac
    16141 cttaccaact tgtttaaaac caagtttttc aaaaatatgt tgcataacct tgttttcagc
    16201 atgcgtatct gagcgaaaat caagataatc aaaaccttca atcaagccct ctaagaaggt
    16261 ttgagcaact ccttttccct gcacatctgc tgccacagca atacggtgaa agactagata
    16321 ctctgactct ccagcttgcc agtttccctc ataaatagct tcataggcct cctctggact
    16381 cttggtcaca gcagcatagg ctagcagttc tccctcttcc aaggctacgt aggcttgccc
    16441 tgagatgata tcatcgataa taacatctgc atttggatag ccattttgcc actggtcact
    16501 accagcatct gctaaacatt ttttggcatc ctccatcacc tgcatgatgg catctacttc
    16561 atttggaaaa gctaaacgaa tttccatctt ttttcctcat ttctgactag tttctcccat
    16621 catagcataa tttaagtatt tctacaagca gttaaaactt gactttgtgt ttttgttatt
    16681 ttataatcta gttatcaaaa ctatataaaa aggagttgaa aatgaaaact accttttcct
    16741 acccaaaatg ggcagaaatt ccaaacattg acctctatct ggaccaggtt ttgctctatg
    16801 tcaatcaggt ctgcgcccct atctctccta ataaagacaa gggcctaaca gcatctatgg
    16861 tcaacaatta tgtgaaaaat ggttacctga caaagcctga caaaaaaaaa taccaacgcc
    16921 aacagattgc ccgtttgatt gctatcacaa ccctcaagtc tgtattttct attcaagaaa
    16981 tagcccagac acttaatact ctacaaactc aagcaagttc agaccaactc tacgatgctt
    17041 ttgtggacta catgaaccaa ggaattgacc cagctaaccc tattatccaa accagctgcc
    17101 aaaccgttaa actctatcat caaactctag acttaatcga tcatactcaa gaggaggtaa
    17161 tccaatgaac actagtctta aactcagcaa acaactcagt tttggagagg agattgctaa
    17221 tagcgtgacc catgctgtgg gtgcagtcat catgcttatc ttgctgccta tttcatccat
    17281 ctatagttat gaagcacacg gatttttatc ctctatcggc gtttccattt tcgtcatcag
    17341 tctctttctc atgttcctat catccaccat ttatcactct atggcctatg gttcgaccca
    17401 caaatatgtt ttgcgaatca ttgaccattc tatgatttac gttgccattg ccggctcata
    17461 cacgcccgtt gtcttgacct tgatgaataa ctggtttggc tatctgatta ttgtcatcca
    17521 atggggaacg accatctttg gtattctcta taaaatcttt gctaaaaagg tcaatgagaa
    17581 atttagcctt gctctttacc tgattatggg ctggttggtt ctggctatca ttcctgccat
    17641 tatcagtcaa acgacacccg ttttctggag tctcatggta actggcggac tctgttatac
    17701 agttggagct ggattttatg ccaagaaaaa accttatttc cacatgattt ggcatctctt
    17761 tatcctaact gcgtccgtac ttcaatacat cgctattgtt tattacatgt aaaaaagttg
    17821 agaaattcaa tctcaacttt tttctttaca catattgata aagtactggt gcaagcgcac
    17881 atcatcagtc aattctggat gaaaagaact taccaacata tttttttctt gggctgcaac
    17941 aatttgattg ttcactgttg ctagaatttc tacaccctca ccaacactac tgataatcgg
    18001 accacggata aaggtcattg gaatcttgcc aactccctta cattctgctt ccgtgtagaa
    18061 acttcctaat tggcgcccat aagcattacg ctcgaccacc atatccatag ttcctagatg
    18121 actctctttc tgagaagtga tttccttagc cagcaaaatt aagcccgcac aggtcccaaa
    18181 cactggtaag ccagatagaa tggcttctcg gatgggaagt agcatgttct ggtcacgtaa
    18241 gagcttgccc atggttgtag actcaccacc aggcaaaatc aaacccgaca agtcactctg
    18301 atcttgctga aaatcatcta gatttctgag ttctacactc tcgacaccta attgatctag
    18361 cacttttgca tgttctgcaa aggccccttg caaggccaat attccgattt tcatctattt
    18421 tcctcgttca gccatgagaa tttggatttc attttcatta ataccaacca tggcttctcc
    18481 taaatcttca gagatttgag ctaggatttg aggattacgg aagttagtca cagccttaac
    18541 aatggcactc gctcgtttaa caggatctcc tgacttgaaa atacctgaac cgacaaagac
    18601 cccctctgcc cctaattgca tcattaacgc agcatctgct ggcgttgcaa cacctccagc
    18661 agcgaaattt acaactggca attttccatg ttcatgaaca tattggacca attctacagg
    18721 gacttgcaaa tccttggcag caacataaag ctcgtcctca cgtaagtttt gaatgcggcg
    18781 aatttcctga ttcatcatac gcatatgacg aacagcttgg acgatatccc ctgtccctgg
    18841 ttctcctttg gtacgaatca tggaagcacc ttcagcgata cgacgcaagg cttcacccaa
    18901 atccttagca ccacagacaa aaggaacttg gaattctttc ttgtccacat ggaaacggtc
    18961 atcagctgga gatagaactt cactctcgtc gatataatca atttcaatag cctctaaaat
    19021 ctgagcttca acaaaatgcc cgattctgac cttagccatt actggaatac taaccgcttc
    19081 ttggatttcc ttaatcatct ttgggtcgct catgcgggaa actcctccag ctgcacgaat
    19141 atcagccgga attcgttcca aggccatcac agctgccgca ccagcagctt ctgcgatacg
    19201 agcctgttca ggattctgca catccataat aacaccaccc ttgagcatct gtgccaagtt
    19261 tttatttagt tcataacgat tttcagtcat ttgttttatc ctcatcattt gtattggtag
    19321 ctaccttttc attttaagtc agaacagaat gaagcacaag ataaaaaaac tataattgta
    19381 tggggacaga ttggctaaaa actatctgac cttaacttaa ggccagatag ctcattcatt
    19441 tttatttttc agccgtaagg gcagccattg tgatgtagtt gtatggttta ttgaagtgtg
    19501 gcaagaagaa gaggtctgtc aatgccaatt tatcaattgt cacatgctct tggatagcaa
    19561 gtgagaacat gtggattccc atgctaattg caatatcatg tgaaaccatt tgggcaccaa
    19621 gaatttcacg gctatctttg tcaaagacaa tcttaattgc tacttcatgg ttgtcatgtt
    19681 tcatgaattc tggtttttga aaatcgttaa agcctgtttc agttgcgttg taaccagcag
    19741 ctttcgcttt ttcaagagtc aaaccagttg aaaccatgtg aagaccgtag attgagatac
    19801 catttgaacc ttgaacaccg attccttcca attcatgtcc acaagcattg taggcaccaa
    19861 cgataccagt gcgaacagca tttgaagcaa gagcgatata gcttgtatct ttacgagcat
    19921 tgtcataaac agtcgcacag tcaccaacag cgtaaacgtc tgggatagat gtttcttgtt
    19981 tcttgtctac aaggaaggca ccgttgcgga agagttcgat cttaccacct gcaagggctg
    20041 tgtttggacg gaaaccaact gcaaggataa ccatatccac gtcaaagctt tctttgtcag
    20101 taatcaagcg ttcaacttta ccgtcacctt cgattgcttt aacagtttga cctagagcca
    20161 agcggatgtt gtgatcttcc aagttcttcg ccatcatttg tgtgaagtct ttgtcatagt
    20221 aaccgttcaa gacagtatca acgatatcaa caaggacaac ttcttttcca agacgttcaa
    20281 aggcttcagc aagttcaaca ccgatgtaac caccaccaac aacggcgata cggtcgaggt
    20341 gttggctctt gtcagaaagt ttattgataa cttcttcagc attttggtac aatttcacga
    20401 attgtacgtt ttcaagagtt gctttaaatt cgcggtttcc tttaacaatt tcaacacctt
    20461 cgattggtgg caagattggt gtagaacctg tagcgaaaat caatttttcg tatgattctt
    20521 tgtgctcttt tccttcaact tccgctgtaa ctactttgtt atcatagtcg attgaaagaa
    20581 caggtgagtt catgtaaact ttagcacctt tagcttccaa tttttcttta tcagaataga
    20641 acaagccttc agcaccgtca atttgttcac caatccaaag agccattcca catcctagga
    20701 aagagatgtt agagttttgg tcaaatacaa caatttcgtt ctcatttcca aaattatcca
    20761 acatggtatt gatacatgct gtaccagcgt ggttagcacc gactacaacg attttactca
    20821 taaaaatttc ctgccttcat tctaaattta ccttactagt ataccattta ccgttagcgt
    20881 ttacaagggg ggtactatta tttcagcatt ttttatcaaa aataattccc catttatcct
    20941 gttttcaagc ctgatgaatt ttatgagcat atttcttgac tttttccaaa ttttgtttgt
    21001 gaaaattcta actttatggt attataataa catgaaaatg aaatgtaagg gcttcaattt
    21061 agatgattat ttgatattac aacttatttt ctataagaga aaggagttgg tagcttgact
    21121 cttcgttcac attctgaacg gctaatgggg actactatca ctatttcatt agtggatgag
    21181 caagccgata tctttctcca aaaatccttt gacttgctca aagaacttga ataccgcttc
    21241 aacgccaata gtcaagaatc tgagttgatg gaaatcaatt atcaagcagg aatagccccc
    21301 gtcacggttc atccagacct gtttgagctg atttcacttg ggttagagca tagcctagcg
    21361 ctctctagcc accttaatat cagcattggt cccttaattc aaacctggcg tatcggtttt
    21421 tcagatgcca aggtcgccca gcctcaagaa attgaatcgg tgctgcctct aatcaatcct
    21481 catggtatcg agttagattc ttctacttct actgtgtttt taaaacagaa aggaatgaag
    21541 atcgatctag gttgtttagc caaaggatac agtgcggata aggttgccca atttcttagg
    21601 aaagaggggg tgacttctgc cttgatcaat ctgggaggga atatcctgac cattggaaaa
    21661 aatcaggcaa gaggggataa cccatggcaa atcgggattc aagacccagc caatcctagg
    21721 ggaaatcatt taatgaccat ccctgttgtc aataaatctg tcgtgacttc aggcatttat
    21781 gaacgtcacc tgaccgtcga tggacaagat taccatcaca tttttgacag tcaaacagga
    21841 tatcctgttg aaacggaact agcgagtcta acaatcatct ctgataaatc agtcgatggc
    21901 gaaatctgga cgactcgact ctttggagaa agacctgctt ctatcctctg gcaagtcgaa
    21961 agtttggagg gcatcgaagt catcctcatc gataaagaag gccacctaag ctgttcttca
    22021 ggaattccta ctctatagag acaactgttt caatggaatt ttaaaattgt attatgtaaa
    22081 aagagaaagg aaatctcatg ttaaaactta ttgctattgt tggaacaaat tcaaaacgtt
    22141 ctacaaaccg tcaattgctt caatacatgc aaaaacactt tactgacaaa gctgaaattg
    22201 aacttgttga aatcaaggcc attcctgtct tcaacaaacc agctgacaag caagtacctg
    22261 ctgaaatatt ggaaattgct gctaaaatcg aagaggcaga tggcgttatt atcggtactc
    22321 ctgagtatga tcactctatt ccagctgttt tgatgagcgc tcttgcttgg ttgtcttatg
    22381 gtatttaccc acttttgaac aaaccaatca tgattacagg tgcttcttac ggtacgcttg
    22441 gttcatctcg tgcccaattg caacttcgtc aaatcttgaa tgctcctgaa atcaaggcaa
    22501 atgttcttcc agatgaattc ttgctctcac actctcttca agcatttaac ccaagtggcg
    22561 acttggttga ccttgatgtt atcaagaaat tggatgccat ctttgatgac ttccgtatct
    22621 ttgtaaaaat cacagaaaaa ttacgtaatg cacaagaatt acttcgcaaa gatgctgaag
    22681 actttgactg ggaaaatttg taagatagga gaccaaaaag aatgaaattt gttggacttg
    22741 ttggatcaaa ctacgatcaa tcatataacc gtaagctctt ggaatttatc cgtcgctact
    22801 tcaaactcaa atttgaatta gaagttcttg aaattgacga agttccaatg tttaaccaag
    22861 atgaaaaatg ggatgaaagt ttccaattac gttatttata taacaaaatt actcgtgcag
    22921 atggtgttat cattgctact cctgagcata accacactat ctcagcttca ctcaaatctg
    22981 tacttgaatg gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg
    23041 gtgcatcata ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg
    23101 atgctccagg tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta
    23161 aggaagcttt tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa
    23221 cttgcttaga taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac
    23281 caatcgaatc agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg
    23341 accctgacga tccagaatgg gtagaaaaag ttgcagcatt cactggtgct gtttctggtg
    23401 atacctatgt caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga
    23461 aagctatgcc atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca
    23521 acgctcacca agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc
    23581 gtatgtcgac tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta
    23641 tcggaacact tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg
    23701 caggtgttat caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg
    23761 gtatcaatga aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg
    23821 gtcaacgttt ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg
    23881 ccgatgctac ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag
    23941 acgctacagc cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt
    24001 tagcgaaaaa agactagcaa acacaaactg ctagtctctt tgttatcgtt tcatctatct
    24061 ttgttaaaag aaagaataca tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    24121 cgcaggctgt acttaagtac ggcaaggtga agctgacgtg gtttgaattt gattttcgaa
    24181 gagtattatt atgaatttgg atcatcaaga ctacggccat gtaatccttt ttcacgttgt
    24241 acttggcgta gtttttctgg tgtaacatcg tttccttctt cgtccacaat tttgattcct
    24301 tcaatgtggt gacgaacagc gcggcgataa ccttcgatgt actcctcacg tagtttggct
    24361 tgttccactt tttcttctgg tgttaagcct tctgtttttt tctttttagc aagctcattg
    24421 atacgagcaa tttttttcgg atccatttct tctcctttgt aataagataa agtccgttta
    24481 tcaaatttta tttagtgtta atttggttaa aacaagcaaa cttagctgct ttcttggtta
    24541 gttgtgacaa gattgccaaa cgattttgac ggacagcctg atcttcagcc attaccatag
    24601 tattttcaaa gaaagcatca atgactgggc taagcgcaaa gagttgtttc aattgctgac
    24661 ttgcaggtcc tgataaaacg agtgtttcta ctgcttctgc caaagctttt tcttggtcat
    24721 tctcaaatag tgctgaatca actgtagcaa ccccttctgc cttctcagcc aggttaaagg
    24781 cacgagaaag tgattcaaca gatggtttaa aatcttcttc cttgcttact tctacgagag
    24841 cacttgctgc ttccaacata tctgccacaa caaagtttga acctgcaaga actgcttcct
    24901 tgatatcttt tggagtagag cccatcatct tatcaacacg agccttgata aagtccataa
    24961 cctctgcttt attttcataa gtcaaactgt caaatttcaa tgcataaagg ctatcaatca
    25021 gctcatccat agcaatgtgc caaccaaagg catccaagat acgaaccaca ccttgagttg
    25081 cacgacgaag ggcataaggg tcattagaac ctgatggaat caatcctact gagaagaaac
    25141 tcaaaatcgt atccaatttg tctgcaatgg ctagaactgc gccgaccttg ctctctggaa
    25201 gttctccttc agctgatgta ggcatgtagt gttcacgaat agcagctgcc accgctggag
    25261 tttcaccagc aagaagggtg tatttttcac ccataattcc ttggagttcg tcaaattcac
    25321 caaccatacc tgtcaacaag tcaaacttgt aaatggctgc tgcacgagca aggtcaactg
    25381 tttcatccac tgacaaacca gctttttctg ccaaaagtac agtgatttga cccgtacgaa
    25441 tcatgtgttc acgaagggaa ccaatcttct catggaaggt gacattgttt aatttttcaa
    25501 caagatctga aatcaccaat ttttggtctt cacgccagaa gaattctccg tcttccaagc
    25561 gggctactaa gactttttca tttcctttga tgacattttt caaacgctct gcgtttccgt
    25621 tacgaacaga aatgaagttt ggcaagagtt ttccatcttg atcacgaaca acaaagtaac
    25681 gctggtgttc cttcatagaa gtcaccaaga cttcttctgg aacttcaagg tatttagcat
    25741 caaaacttcc catgaaggca gttgggtatt caaccaaatt caagacttca ttcagcaaat
    25801 ccgcatcaat ttcgatacgt acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa
    25861 tcatttgctc acgttcacat ggatctgcga ttacaaactg cttacgaagg tcttcttcat
    25921 agctcaatgc tgactgaatt ttggtttctt tccccaagaa acgatggcca cggctcacac
    25981 gacttccctt gatatcaagg aaatccaagt caaactcttg ctcatccaag agaacagtta
    26041 aagtgtgaac agggcggatg tattcaaagc tatttccagc ccagtgcatg ctgacaggga
    26101 aagtcaatga cttcaagaca tctacaacac caggaacaat ggcttcaact gattggccaa
    26161 cttcttcctt agtgacatag acatattctt cacccttaat ttcacggaat tcgatatctt
    26221 caacagtcaa acctttccca cggacaaatc cttgagctgc tttggtgaag tttccatcac
    26281 tatccaaggc aattttcttt gctggtccct tgaaatcttc tgtcaaatca gactgtttgt
    26341 ctgataaacc agtcacacga acagccaaac gacgtggtgt tgagaaggtt tgaatggctt
    26401 caaaagacag gcggttttcc ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac
    26461 ttggtgtgac aacataggct ggtaattctt caagaccgag ttctactaat aagttttttg
    26521 tcatgttttt tctccgaaaa gtatcttttt attttccagt ttgccttatg tgattggcac
    26581 gcaagcaacc aacttcgttg tttcattgct aaaattggag ccaaaagtct ccaattttgc
    26641 ctagtatcct tagtttcact aaggatacct tcatcactgc ggcaactggc tcagggctca
    26701 ctctgttcgc ccattttcgt aatttgtcac tctctttatt ctgcgtcttc tgctaggagt
    26761 ttagctcttg tttcttcatc caaaagtggg tagcctaggc gtttgcgttc tgcgacaaag
    26821 gttttggcta cgacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt
    26881 acagatacgg caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag
    26941 tcataggcag ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta
    27001 tcaaagtttt caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca
    27061 tactcaggct ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag
    27121 acagagtcta cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca
    27181 gtcacagggc cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc
    27241 attccgtcaa gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag
    27301 ttgtcctcaa caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagat
    27361 tcaaggtaaa gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg
    27421 tgttggtaga gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc
    27481 tctacataag ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc
    27541 attgttcccg cacctttttc attatcataa gcctgcataa gcatacaacc ttggtcattc
    27601 caaaattgtt gcaaagtaag gatgatttcc tggaaagtca atttcttaga cattgtttac
    27661 tcctttttat ataaattact tgcgacatga gtctgcctcg tcgatcatac gaggcaagac
    27721 gagttagtac tgcgtctagc tcaggcactc tagtgctgag cgtggggcag tccgactgta
    27781 aggaggtttc tgccactgac gcagtgaaat gtcaatttct tagacattgt ttactccttt
    27841 agtttttata tttctttttc aattaatttc tgctgaacaa accaatctaa caagggaaaa
    27901 gttttttctg tatttgccca taaatgataa gaatctaaga ccgcttgtac actacctaaa
    27961 ttttctacca acttatctac tgtcaaaaag ctgcgccagt attcataaaa aatacttgca
    28021 tagttcttat cataagtcga agaaccaaaa tcattcaatg aatgccaacc atacttcttc
    28081 tgaaaaagtt ctacgagaga ttgattacaa attttttccg cttgaaattc ctcttctgtc
    28141 aagaaatact tgcgactaat atattcaacc atcccctctt cgaaccagat ataagagtca
    28201 taaccatcaa aatcatctaa aaaatgctcc gaccaatgag ctaactcatg tcctacaatc
    28261 tgtaagagga aattttcaga taaagaatga tagtgacttt ctattgcttg agtttgctga
    28321 gaacactcgt agtccatcaa ttgatacaaa tacaactctt tccaaacagc taaatcaggc
    28381 gccataacca ttcgtctatt atttgtatag gctggaacag cgctttccct aataatctgt
    28441 gtagcagcat taaaatctga ccaaataacg gcttgcggta aatcacagac tgcaagttca
    28501 ttctttaaaa aagattgata atctttcaac ttttcagtat ttcttactac aaaatcacga
    28561 aatgtagcta gttgactatc gtcttttaca agataaaggt tgtccacggc tctcctttct
    28621 ttcacacaat agactgaata agcaaaagaa ccgcgtccga caatcctagg ttgatttaac
    28681 ctaggggcgt cagaaacgcg gttccaccct aatttattac ttcattgttt ttgaaaatac
    28741 tacagaaagc gccagctcat tattttgccc tacttggctc ccactatccc aagctcgctt
    28801 gaagaacgcc ataagacttt ctttcctgct gactattata tcaaaaatta gaataatgta
    28861 caattctttt tatgattttc tagaaatcca catcatcaac tcgtggagca cccgattgaa
    28921 cagcaatcgt ttttaagttt tctctctcct catgactcag ttcaattcca aagcaatcaa
    28981 gattagcttg aatacgagag gttgtgacag attttggaag tggtaaaaat ccttctgcca
    29041 agctccaggc caaggctatc tgagcaatcg attttccgtg atttgctgcg atttcttgca
    29101 cttgcttgct atcaaatagt tctccctgac caaaaggccc ccaagcttcc aataaaattc
    29161 ccttttcacg acagtaagct acgacttgat cttgatacac acctggcgcc aagcgaactt
    29221 gattgaccgc aggaacgata gttgcagttt caagcaaggc atccaaatga tggggaagaa
    29281 aattgctaac gccgatagca cggattttcc cttcttgata gaggtcttcc atcgctctcc
    29341 aaacttccgc attgcgagtt ttccatgcgt cattttctct gagcggtttt gggttcggcc
    29401 aatgaatcaa atacaaatcc aaataatcca agcccagttt ttctatagat ttttccaaag
    29461 cttgacgagt ttgctcatag gtttgctgac tattccaaag cttggtagtt acgaacattt
    29521 cttcacgtgg aactccgcta tctttgattg cttgaccaac actttcttca ttctgataaa
    29581 tcgccgccgt atcaatatga cgataaccag ccttcaaggc ttctaacact gcacgatagg
    29641 cttcttctcc atccttagcc ttaaaagttc caaatcccaa tactggaatt tctactccat
    29701 tatttaattg ataggtattc atatgcttct ccttctattt tattcatcaa ttatagcata
    29761 aaacattttt taagtagtac tatcttctgt aaaaaataag tctgtcaaat atctaacaga
    29821 cctatttttt cttgcttatt catcacgact atagtacagc tcatgagctt ttaggcgagt
    29881 attgagcatc tctggtatgg tcacaaaggt ataaccttga tttttcaaat actcaatgac
    29941 ccttggcaag gcattgactg tcggactgtg aatatcatgc atcaaaacga tagagccgtt
    30001 agctacttga tgctgaattt ctgtcaaaat agatgcttca tttttactct tccagtccag
    30061 actatccaca tcccacatga taaagctcaa atccaagcta ttgcgaatat catctgtaat
    30121 agcaccataa ggtggacgca tgagtttaga actagaaccc agcactttag ttagcacatc
    30181 ctcagtatca gtaatctgct ttttagcttc atcaagagag agttgcgaga gaatcggatg
    30241 gctccaacta tggtttccaa caacatgacc ttcagatttt atcctcttca ccaagtcctc
    30301 attcccagaa acatttttcc caagcacaaa gaaagtcgct ttaatatcat atttagctag
    30361 ggtctctaat acctgcgggg tcgttgctgg atttggacca tcatcaaagg ttagagcgac
    30421 aactttttga tatttcttat caaagtaaga ttggtacaag gccgcatctt tttcgagtaa
    30481 gtacgaagat tggataacat caaagaaagc agatactggc aaggctatct cttctaaatt
    30541 ttcaaccaca ggacttggat aaaggataat ctgactatcc ttgtaatcaa aattccatgc
    30601 agacaagtct tggtcagaga agttttttac aatctgctca ctctggtctt gctctatttt
    30661 tttatcctct atgaaggagg tcaactcttt tatcagctgt tccttagcct tactagcatc
    30721 tgaaaacagt tggtcaagtg taaaaggttg gccatcttct gttaagtgaa cctttcctag
    30781 actggtcttt tcagtctctt caaccttaaa agcaactaaa tcatagattt gtttggtcac
    30841 attacgatta acgactccct ttaaacctga ctcttcttgc tctgtatagt agaaaacaag
    30901 attgtcctta ctttctagct tgtccttaac atcttgattt atcaactccc taacagagga
    30961 aatcactttc tccccttgga gaggataata ggcaatcact tcggcttgcc cctgacgaaa
    31021 atgctccttc tgatttccct cactcaattg atcatctttc tcttttttga gcgattcaat
    31081 cttttgctca aaactttttt gctggtagat cttaaaagca ataaatccgc ctaataggca
    31141 aatagaaatt gctaaaatac caatcaaagc caataataca tgtctcgttt tcccgtgtct
    31201 gccacgtcct agtctacttt tattcatatt tatatcataa caaatttgag caataatttc
    31261 aatgatacaa tttggcaatc cacaattcct ctagcaagcc aaagcatctt acatcaactc
    31321 caacatccgt atctgtagag attatgtagt tttctatcta tggaatttct agtagattga
    31381 agctagaata gtagacctat acttctaaaa cattgttaga aatcgatttg attgtcctga
    31441 tcgatttgtc ctgttctcat ttcattttac tataaaacta tgaaaagcta ggtcatctga
    31501 gaagtaatta ctatcgacaa ttgcgatagc gtatactggt gcgatttgtt tgtagctctg
    31561 gtgggtatca ccttcacgtt ggcgaatttt ttctaggttt tgattaacct gactgcacag
    31621 ataagcccat aggcgattga tgaaaaaatt ctgatgatga acctgaattt cgatgataac
    31681 ttggattcca ttatctaact ccgccaagac gtctatacta gtatagaaat cttgcgctga
    31741 gtagggcatg gaaggcaaga cgtgaatgtt acttccctcc aaaatcgtca cattttttgc
    31801 tggcaagtcc agcatatcgc ggataaattg acaaatcatt agttgggctg atacctggat
    31861 gtctgagaat cctccttttc ctccttctat tatagcacat ttttaaatct aggtttgcta
    31921 gattctatgc ttctatctat ttattcggaa aagaaagcta atagcttatt tgaaattttc
    31981 tcctctctgt tggatagtcc cgtctatcta tgttataatg aaagaaggat ttaaaatcgg
    32041 aaaaggagta tttatgctaa aattaggtgt catcggaact ggcgctatca gccatcattt
    32101 catagaagca gcccatacca gtggagaata ccaactggtc gcaatctatt ctagaaaact
    32161 agaaactgca gcaacctttg cttctcgcta tcagaatatc caactattcg atcaattaga
    32221 agtcttcttc aaatcttctt ttgatttagt ctatatcgcc agtccaaact ccttacattt
    32281 tgctcaggca aaagctgcct tgtctgctgg taaacatgtt attctcgaaa agccagttgt
    32341 cactcaacca caagaatggt ttgatttgat tcaaacagct gaaaaaaata actgttttat
    32401 ctttgaagca gctcgtaatt accacgagaa agcctttact actatcaaaa actttttagc
    32461 agataagcaa gtgttaggag cagatttcaa ctatgccaaa tactcttcca agatgcctga
    32521 cttgttggct ggacaaacac caaatgtctt ttcagaccgt tttgctggtg gagccctcat
    32581 ggatttgggg atttatcccc tctatgctgc tgttcgtctt tttgaaaaag ctaatgacgc
    32641 gacttaccat gctcaacagc ttgacaatag cattgaccta aatggagatg gtatcctctt
    32701 ctacccagac tatcaagttc acatcaaggc tggaaaaaac atcacttcca atcttccttg
    32761 cgagatttat acaacagatg gaaccttgac tctcaacacg attgagcata ttcgctcagc
    32821 tatttttacc gaccaccaag gaaatcaagt ccagcttcct atccaacagg ctcctcatac
    32881 gatgactgag gaagtcgctg catttgcaca catgatccag caaccagatc tgaatctcta
    32941 ccaaacttgg ctgtatgatg caggctctgt tcatgagcta ctatatacca tgcgccagac
    33001 tgctggcatt agatttgagg cagaaaaatg aaaaccaaac taccgactga atggcaagaa
    33061 ctgagtgacc aactcggttt ccaagaattc acccccattc aaactcaact atttgagccc
    33121 cttcttgctg gagaaaacct cctaggagtg agccaaaccg gaactggtaa gaccctagct
    33181 tatctcctac caagtcttct cagactacaa aagaaaaaag cccaacaact cttgattcta
    33241 gcaccaaata cggaacttgc tggacagatt tttgatgtat gtaaaacgtg ggcagaagct
    33301 atcggattga ctgctcaact cttcctttca ggttcaagtc agaaacgaca aattgaacgt
    33361 cttaaaaagg ggccagaaat tctgattgga actcctggcc gtatctttga actcattaaa
    33421 cttaaaaaaa tcaagatgat gaatgtggaa accatcatcc tggatgaatt tgaccaatta
    33481 ctagatgatt ctcagattca ctttgtagaa aaaatcactc actacgcacc tcgtgaccac
    33541 caactggtct acatgagtgc cacgaccaag tttgatcaag aaaagattgt accgaataca
    33601 cgcactattg atctctctga tcaaaaattg gacaatatcc agcatttcta catgcaggta
    33661 gaccaacgtc atcgagtgga tatactacga aaactggctc atgtagagga tttccgtggt
    33721 ctagtcttct tcaatagcct gtcagacctt gggaatgccg aggaaaaact acagtatcgt
    33781 gatatattgg ctgtttccct cgctagtgat gttaatgtta aatttagaaa agtcatctta
    33841 gaaaagttta aagataacca actaaccctg cttcttgcaa ctgacctttt ggctcgtgga
    33901 attgatatcg atagcctaga atgtgtcgta aactttgata ttcctagaga tagcgaaacc
    33961 tacacacacc gtgctggccg tactggccgt atgggcaagg aaggatatgt tattactctc
    34021 gtcactcatc cagaagaaat caaaaaactt aaaaagtttg caagtatccg agaaattgtc
    34081 ctaaaaaacc aagaactcta tatcaaataa agccccattg ctctatacac atccagtgta
    34141 tggagctttt ttacaagcaa ttcaatttta tactcatctg ttttcaaaaa gcattctaat
    34201 ccatctccga ttaacgatgg actttatcac ctccttctcc agttcttgta taatatcttg
    34261 aagttgattc atgacatctt ccaaagttcg aaaggcttta ttcttaaatc cacgtttacg
    34321 aatctctttc cacacttgtt caacggggtt tatctctggt gtgtatggag gaataaatgc
    34381 aaagccaata ttagtcggaa tttttaaggt acttgattta tgccatatag cattgtccat
    34441 aacgaataaa agataatcat ctggataagc ttgtgaaagc tcttctaaaa agacgttcat
    34501 ccactcagta ttacatccac cagctattaa gaaaaatgat tcgcctgtat gggtccataa
    34561 caatagcgaa attctcgtat atagtgacta tggacatgtg gacctactcc tattggagac
    34621 caacaagatc ccagtttact gattctaccg aagccagcct catcttggta catcaagcga
    34681 accttatgaa aaggtctact ggttttaaaa cgctttcttt tcttcttgaa ttgagatttt
    34741 atttttagac gcgacaatgg tttgagcgtc tgctttctta ggatgttctg gacgtggcat
    34801 aatatttcgc caaccatggc gcttcaacag ttgatagaag gcatcacgtg tgtaggaacg
    34861 acctaactcc tttttataag cctgaaataa ggcatcaatt gtaacaaatt ctcctgcctc
    34921 tgcagccttc aaatgacggg caagaaaggc tttctcttcc ccaaccgtca tatatgcatg
    34981 gttacgacca ccac
//
LOCUS       DAVKJU010000018        34174 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVKJU010000018 DAVKJU010000000
VERSION     DAVKJU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34174
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.018"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(56..1117)
                     /locus_tag="VW191_000784"
     CDS             complement(56..1117)
                     /locus_tag="VW191_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU9623575.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLFPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            complement(1110..3941)
                     /gene="uvrA"
                     /locus_tag="VW191_000785"
     CDS             complement(1110..3941)
                     /gene="uvrA"
                     /locus_tag="VW191_000785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU9623576.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALHIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            4073..5017
                     /locus_tag="VW191_000786"
     CDS             4073..5017
                     /locus_tag="VW191_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU9623577.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            5029..5712
                     /locus_tag="VW191_000787"
     CDS             5029..5712
                     /locus_tag="VW191_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEU9623578.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            5967..6998
                     /locus_tag="VW191_000788"
     CDS             5967..6998
                     /locus_tag="VW191_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU9623579.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVILFDYNK"
     gene            complement(7135..7812)
                     /locus_tag="VW191_000789"
     CDS             complement(7135..7812)
                     /locus_tag="VW191_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9623580.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLSGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(7809..8372)
                     /locus_tag="VW191_000790"
     CDS             complement(7809..8372)
                     /locus_tag="VW191_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU9623581.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            complement(8382..8975)
                     /gene="ruvA"
                     /locus_tag="VW191_000791"
     CDS             complement(8382..8975)
                     /gene="ruvA"
                     /locus_tag="VW191_000791"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU9623582.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKIAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     regulatory      9132..9246
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            9380..10480
                     /gene="ribD"
                     /locus_tag="VW191_000792"
     CDS             9380..10480
                     /gene="ribD"
                     /locus_tag="VW191_000792"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU9623583.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGSHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMTMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     gene            10465..11100
                     /gene="ribE"
                     /locus_tag="VW191_000793"
     CDS             10465..11100
                     /gene="ribE"
                     /locus_tag="VW191_000793"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU9623584.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDANAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            11120..12325
                     /locus_tag="VW191_000794"
     CDS             11120..12325
                     /locus_tag="VW191_000794"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU9623585.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYRITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            12326..12793
                     /locus_tag="VW191_000795"
     CDS             12326..12793
                     /locus_tag="VW191_000795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU9623586.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(13118..15067)
                     /gene="mutL"
                     /locus_tag="VW191_000796"
     CDS             complement(13118..15067)
                     /gene="mutL"
                     /locus_tag="VW191_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU9623587.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLASA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            15423..15863
                     /locus_tag="VW191_000797"
     CDS             15423..15863
                     /locus_tag="VW191_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623588.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            15860..16318
                     /locus_tag="VW191_000798"
     CDS             15860..16318
                     /locus_tag="VW191_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEU9623589.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            16628..17890
                     /locus_tag="VW191_000799"
     CDS             16628..17890
                     /locus_tag="VW191_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU9623590.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(18183..18653)
                     /gene="nrdI"
                     /locus_tag="VW191_000800"
     CDS             complement(18183..18653)
                     /gene="nrdI"
                     /locus_tag="VW191_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEU9623591.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(18731..19264)
                     /locus_tag="VW191_000801"
     CDS             complement(18731..19264)
                     /locus_tag="VW191_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623592.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            complement(19316..20602)
                     /locus_tag="VW191_000802"
     CDS             complement(19316..20602)
                     /locus_tag="VW191_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623593.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            complement(20614..22260)
                     /locus_tag="VW191_000803"
     CDS             complement(20614..22260)
                     /locus_tag="VW191_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9623594.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            complement(22262..22876)
                     /locus_tag="VW191_000804"
     CDS             complement(22262..22876)
                     /locus_tag="VW191_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEU9623595.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            23011..23580
                     /locus_tag="VW191_000805"
     CDS             23011..23580
                     /locus_tag="VW191_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9623596.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPHFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            complement(23609..24301)
                     /locus_tag="VW191_000806"
     CDS             complement(23609..24301)
                     /locus_tag="VW191_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623597.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(24303..25364)
                     /locus_tag="VW191_000807"
     CDS             complement(24303..25364)
                     /locus_tag="VW191_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEU9623598.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            complement(25357..26730)
                     /locus_tag="VW191_000808"
     CDS             complement(25357..26730)
                     /locus_tag="VW191_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU9623599.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            complement(26830..27684)
                     /locus_tag="VW191_000809"
     CDS             complement(26830..27684)
                     /locus_tag="VW191_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9623600.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            complement(27838..28668)
                     /locus_tag="VW191_000810"
     CDS             complement(27838..28668)
                     /locus_tag="VW191_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623601.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKDLYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            complement(28772..29095)
                     /locus_tag="VW191_000811"
     CDS             complement(28772..29095)
                     /locus_tag="VW191_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEU9623602.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLAF"
     gene            complement(29085..29741)
                     /locus_tag="VW191_000812"
     CDS             complement(29085..29741)
                     /locus_tag="VW191_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEU9623603.1"
                     /translation="MPTALGYVSIGLACGIIGAPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIAVKAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAILFATLLGCSMGVVLDGQ
                     "
     gene            29812..29889
                     /locus_tag="VW191_000813"
                     /pseudo
     CDS             29812..29889
                     /locus_tag="VW191_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30196..31422)
                     /locus_tag="VW191_000814"
     CDS             complement(30196..31422)
                     /locus_tag="VW191_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9623604.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLFVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(31494..32159)
                     /locus_tag="VW191_000815"
     CDS             complement(31494..32159)
                     /locus_tag="VW191_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9623605.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(32227..32427)
                     /locus_tag="VW191_000816"
     CDS             complement(32227..32427)
                     /locus_tag="VW191_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEU9623606.1"
                     /translation="MLNLQFAETMELTEAELETVYGGECGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            complement(32727..33590)
                     /locus_tag="VW191_000817"
     CDS             complement(32727..33590)
                     /locus_tag="VW191_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9623607.1"
                     /translation="MIERMELGEFYKELRLARKLKQSDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRKVVDRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            complement(33985..>34174)
                     /locus_tag="VW191_000818"
     CDS             complement(33985..>34174)
                     /locus_tag="VW191_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9623608.1"
                     /translation="LIVGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVVNRWDR
                     GLEAYKDKVYTRGIWIRD"
BASE COUNT        10401 a         7431 c         6550 g         9792 t
ORIGIN      
        1 ttttaatttt ttttgacttt tttcgtcatt ttcgtacttt tttcttgaca aacaactaaa
       61 tgactatcaa ctcttttgga gctagggtca ataattcaca acctgtctct gtaatcagga
      121 tatcatcctc gatacgaacg ccatatttgc cttcgatata gatacctggt tcatcggtca
      181 aggccatacc tgtcttaata gtttctgtag aagtctggct aaagtagggt tcctcatgga
      241 tatccagacc aataccgtgg ccaatgccgt gagtaaagta gtcgccataa cctgcctcaa
      301 tgataatatc acgagggatt ttgtcaaagt cacggaaacc taagcctgcc ttagcttggt
      361 caatcaaggc ttggttagct tttagaaccg tattgtaaat ctctgcctgc tcatcgctaa
      421 catgccctag atagatagtc cgggtcatat cactgacata gtggtcatag agacagccga
      481 agtccatggt aatggcttct cccaactcca ctggtttgtg cattggatgg gcatggggtt
      541 tagaagaatt gataccgcta gctaggatcg tatcaaaaga taagccagat gctcccaact
      601 cacgcatgcg gaagtcaagg aagttggcaa tctcaatttc agtttttcct ggtttgataa
      661 agtcaagcgc atcgcggaaa gcttggtctg agatagaaca agccttgcga atcgctgcaa
      721 tctctgcctc atccttaatc atacgaagac cttccacaaa ctgagtttgc ggaaacaagt
      781 ccaaacctgc aaaagctgcc tgcatacggt ggtaataaga cactgaaatc tcatcttcaa
      841 aaccgatacg agtcaagccc atgtccttaa caattcctgc aatgacagcc aattcatcac
      901 gatcagccac aatctcaaaa ccactggttt cttgcttagc tgcgatgata tagcgagagt
      961 ctgtcactaa gacctgacgg tcacggctga taaagactgt tccgtttgag ccccaaaaac
     1021 cagtcaaata atagacgttt ttaagattgt tgatgatgac accatctagt tctttttctt
     1081 gcattttagc tagaaatgct tgtacgcgtt tattcatgat gtaactttcc tttcaaatag
     1141 tgtcctgtat agctggcttc gttggcagct acttcttctg gagttcctgt tacgatgatg
     1201 gttccaccac cgacaccgcc ctcaggtccc aagtcaatga tatggtctgc cgtcttgata
     1261 acatccagat tgtgctcgat gacgaggact gtattgccat cgtctacaaa gcgagctaaa
     1321 accttgagca ggcgagcaat gtcctctgta tgaagccctg tcgtcggctc atccagaatg
     1381 tagaaagatt ttcctgtcga gcgtttgtgg agttcgctag ctaacttcat acgttgggct
     1441 tctcccccag aaagggtggt agctggctgt cccaaggtca catagcctag ccctacatcc
     1501 ttgatggtct ggagtttgcg ttgaattttc ggaatgtgtt ggaaaaattc taccgcatcg
     1561 ttgaccgtca tatccaagac ctgcgaaata ttcttttcct tgtagtgaac ttctagggtt
     1621 tcactgttat agcgggttcc gtggcaaact tcacaagcca cataaacatc tggcaagaag
     1681 tgcatctcaa tcttgataat cccgtcacct gagcaagctt cacagcgacc tcccttgacg
     1741 ttgaaactga agcgcccctt cttgtagcct cgaatcttgg cttcatttgt ctgagcaaaa
     1801 aggtcacgta tatcgtcaaa aactcctgta taggtagctg ggttagacct cggcgtccgt
     1861 ccgatagggc tctggtcaat atcaatcaaa cggtcgacat gctcaatccc tgtaatagtc
     1921 ttaaacttac caggtttgtc tgaattacgg ttgagcttct gggcaatggc ttttttgaga
     1981 atgctgttga ttagagtcga tttccctgaa cccgacacac ctgtcactgc gataaatttt
     2041 cctagtggaa agcgagccgt gacattttgc aagttgttct cacgcgctcc tatcacttca
     2101 ataaaacgac catttccgac acggcgctct tctggtactg ggatgacacg tttgcctgac
     2161 aagtactgac ctgtgataga cttgctgttg cgagccactt gcttaggtgt acctgctgca
     2221 acaatctcac caccaaaaac accggcacca ggaccaacgt caatcagata atcagcctca
     2281 cgcatggtat cttcgtcgtg ttccaccacg ataagagtat tgcccaagtc acgcatcttt
     2341 ttcagactgg caatcaggcg atcattgtcc ctctggtgaa gaccgattga cggctcgtct
     2401 aggatataga ggacacctga taggttggaa ccaatctggg ttgccaaacg aatgcgctga
     2461 ctttccccac ctgaaagggt tcctgctgaa cgtgacaggg ttagatagtt aagacccaca
     2521 ttattaagga aggtcaaacg atccttgatt tccttgagaa tgggacgagc aatgatggct
     2581 tcattttcag acaaagttaa ctggctcacc aagtccaagt ggtcagcgat agacaggtct
     2641 gagatttctc caatatgtgg cccttgctgg ccgcccacac ggacagacaa ggcctggtca
     2701 ttgagacgat agccttgaca ggttccgcag gtcagctcat tcatgtagag acgcatctga
     2761 gtgcgagtgt aatcgctatt ggtttcatgg taacgacgtt tgatattatt gataactccc
     2821 tcaaacggaa tgtcgatatc gcgcacgcca ccaaattcat tctcatagtg gaaatggaat
     2881 tccttaccat ctgacccata gagaatcaag ttcttatctt cttctgacag gtcctcaaaa
     2941 ggcttatcca tagccactcc aaagactttc atggcctgct ctaacatgtt tggatagtag
     3001 ttggatgaga taggattcca aggtgctagc gctccctcac gtaaggtttt gctagcatct
     3061 ggcactacca aatcagtatc cacctccagc ttgatgccca agccgtcaca ctcactacaa
     3121 gagccaaaag gagcattgaa agaaaagaga cgaggctcta actctgggac agtaaaacca
     3181 caaactggac aggcataatg ctcagagaac aacaactcag agtcgtccat ggtgtcgata
     3241 atgacataac cttctgcaat atgaagggca gcctcaatgg aatcaaagag acgactacga
     3301 atgccctcct tgataacaat acggtcaacc acgacatcga tattgtgttg cttgctctta
     3361 gacaactctg gcacttcggt cacatcatag acttccccat ccacacggac acgaacatac
     3421 ccgtctttct gaaccttctc gataacactc ttatgttggc cttttttctt gcggatgaca
     3481 ggagccaaga tctgcaagcg ctggcgttca ggtaactcca aaaccttatc aacgatttgc
     3541 tccacagaag aagcattgat agctccatgt ccgttgatac agtaaggcgt ccccacacgt
     3601 gcgtagagga gacgcagata gtcattgatt tcagtcgtcg ttcccaccgt cgagcgagga
     3661 tttttactag tcgttttctg gtcgatggaa atagctgggc tgagaccatc aatggcatct
     3721 acatctggtt tttccatatt tcccaagaac tgacgagcgt aggcggacaa actctctaca
     3781 tagcgacgtt gtccctccgc atagagagta tcaaaagcca gactggactt ccctgaacct
     3841 gacaagccag tcacgacaac caacttgtct cgcggaatct ccacatcaat attttttaaa
     3901 ttatgggcac gcgccccatg aatgacaatt ttatcttgca tctttgttct ttctagtcca
     3961 ttattgctta ccattatacc aaaaaaagtg agattctatt acccaaaagg ccgattttgt
     4021 agtataatag tacagtgtga aaaaatctga aaaatgagaa aggataaggg atatgaaaca
     4081 agtttttctc tctacaacaa ctgaatttaa agagatcgat acgcttgaac cgggtacttg
     4141 gatcaatctc gtcaatccga ctcaaaatga atcactcgaa atcgccaaca ccttcgatat
     4201 tgatattgcc gaccttcgag caccgctcga tgcggaagaa atgtctcgta ttaccattga
     4261 agacgagtat accctgatta tcgtagacgt gccggtcacg gaggaaagaa ataaccgcac
     4321 ctactacgta accatcccgc ttggtattat catcactgag gaaaccatta tcactacgtg
     4381 tttggaacca ctacctgtcc ttgatgtctt tatcaaccgt cgattgcgta atttctatac
     4441 cttcatgcgt tcacgtttta tctttcaaat tctttatcgc aatgcagagc tttacctaac
     4501 agcccttcgt tcaatcgacc gcaagagtga acaaatcgaa agtcaactgc atcaatcaac
     4561 tcgtaatgaa gaattgattg agctcatgga attggaaaaa actatcgtct atttcaaggc
     4621 ctccctcaaa acaaatgagc gcgtgattaa gaaattgacc agttcaacca gcaatatcaa
     4681 gaaatacctt gaggacgaag acctgcttga agacaccctg attgaaaccc aacaggccat
     4741 cgagatggca gatatttatg gaaacgtctt gcattctatg acagagacct ttgcctctat
     4801 catttctaac aaccagaaca acatcatgaa aaccttggcc cttgtgacca tcgtcatgtc
     4861 catcccaacc atggtctttt ctgcctacgg gatgaacttt aaggataatg aaattcccct
     4921 aaacggagag ccaaatgcct tctggttaat cgtctttatc gcctttgcta tgagtgtctc
     4981 gctcactctc tatctcatcc ataaaaaatg gttctaagag gagttcctat gtctcaaatt
     5041 gatctacaaa aattaactaa gaaaaaccaa gagtttgtcc acattgctac ccaacaattc
     5101 atcaaagatg ggaaaacaga cgctgaaatc cagactattt ttgaggaagt cattccccaa
     5161 atccttgagg agcaatctaa aggtacaact gcccgttccc tatacggcgc accaactcat
     5221 tgggctcata gcttcactgt caaagagcag tacgaaaaag agcatccaaa agaaaatgat
     5281 gatccaaaac tgatgattat ggactcagct cttttcatca ctagcctctt tgcccttgtc
     5341 agcgccctca caaccttctt tgcggcagat caagctttcg gctatggatt gattactctt
     5401 ctattagttg gactggttgg tggatttgcc ttctacttga tgtactactt tgtttaccaa
     5461 tactatggac cagatatgga tcgcagtcaa cgtccacctt tctggaaatc tgtactagtt
     5521 atcctagctt ctatgttcct ttggttgctt gtcttctttg caacaagctt cctaccagct
     5581 agccttaacc cagtactgga tccattgcca ctagctatta ttggagcagc cctcctagcc
     5641 cttcgcttct atctcaagaa acgcttgaat atccgtagtg caagtgcagg accaacacgc
     5701 tatcaagaat aagaaaacga taaaagcaac tgcaggtgcg gttgcttttt cacttacttt
     5761 tttgagttgt actcaatgaa aatcaaagag caaactagga agctagctgc aggttgctca
     5821 aagcacagct ttgaagttgc agataaaact gacgtggttt gaagagattt tcgaagagta
     5881 ttaaaagtat tcttctgaaa tcccacatag ctttctctta tattttgtga taaaataggc
     5941 tcaatctatt tctaggagga taagatatgg tttctactat tggtattgtt agtttatcta
     6001 gtggcattat cggagaggat tttgtcaaac acgaagtgga cttgggtatc caacgtctca
     6061 aggatctggg actcaatccc atctttttgc cccattcgtt aaaaggatta gactttatca
     6121 aggaccatcc tgaagctcgt gcagaggatt tgattcatgc cttttctgat gatagcatcg
     6181 acatgatcct atgtgccatc ggtggagacg atacctatcg cttgctacct tatctttttg
     6241 aaaatgacca actccaaaag gttatcaaac aaaaaatttt tcttggcttc tcggatacaa
     6301 ccatgaacca cctcatgttg cataaattag gaatcaagac tttttatggt caatcctttt
     6361 tagcagacat ttgtgaatta gacaaagaaa tgctagccta tagccttcac tactttaaag
     6421 aattgattga gacgggaaga atttcagaaa tccgccctag tgacgtttgg tatgaggaac
     6481 gaactgattt tagtcccacg gccctgggaa cacctcgtgt cagtcataca aatacaggtt
     6541 ttgacttgtt gcaaggaagt gctcagttcg aggggaaaat cctcggtggt tgcctcgaat
     6601 ccctctacga tatctttgac aactctcgat acgcagatag cacggaactc tgccaaaaat
     6661 acaaactttt ccctgacttg tcagactggg aagggaaaat cctcttgctc gaaacaagcg
     6721 aagaaaagcc taagccagaa gacttcaaaa agatgttgct aactttaaag gacactggca
     6781 tattcgcggt catcaatgga ctcttggtcg gaaaacctat ggatgaaact ttccatgacg
     6841 actataaaga ggcactattg gatatcattg acagcaatat cccgattgtc tataatctga
     6901 atgtcggcca cgcaactcca agagcaattg taccctttgg ggtccatgct catgtagatg
     6961 cacaggaaca agtcattctc tttgactata acaaataaag ctgggaaaag tttctcagct
     7021 tttttctcgg tatcattact ggcattttca tcacatatta cagtatattg aaactagaat
     7081 agtacacctc tacttctaaa acattgttag aaatcgattt gtcctgttct catttcattt
     7141 tactatactg atagctaaaa tgattaagga gaggctgttg cttaacatgt gaacaagtag
     7201 gggatagtag atattctctt tttctttcac ataaataata gaaaaggcaa ggcctccacc
     7261 taagtaacca actgcaccaa tccactctga tagagccaaa ctgtgcatat gagtcagagc
     7321 aaaaacaagt cctaccagaa taatgcttag taaacctgac aaccgttcct gcaagtaatg
     7381 caataagacc tgacggaaaa ataattcttc taccagaggt ccaatgacac aagcaaaaac
     7441 agctatcagt agtggttgtt cttgaaaggt actttgaata ttagactgat ttagaccttg
     7501 tccatctagt cccacaaact gcttcaacat ctctgataca aattcaaaga caacagtcat
     7561 cagaatgaga aagagccatc ctgttaagac tccaaagaag aattttcttt tagtctttct
     7621 aatctccttc cattgttgga tcaatctatc cttgaacaag aaggagccat ataaagctaa
     7681 aactacataa acaagataat ttgcaatggc agatggcatt aaagaagcca gcaatttaac
     7741 gccatcaaac acaaagagga ctgtatagag aatcgcaaaa atcagaattc ccttattttt
     7801 gttagacatc atttaagacc tttccactca caatcattct cgtggtcatc aactagccct
     7861 gcagcctgta gaaaagacaa tacggcgact gggcctgtga acttgaagtc tcgttttttg
     7921 agatctttgg ctaatttctc agataagggt gttttagctg gggcttggcg ataatcagga
     7981 acatcgttaa cgacagtttt cccctcaaca aaagaccaaa gataggcatc aaaagagccg
     8041 tactctgcct gtaactgtag aaaggcttgg gcgttagcgc gtgtagcaaa aatcttggct
     8101 ctatttcgaa tgatagctgg attctccagc atggcttcca attcagtgtc agtcatctct
     8161 gcgactgagt gaatttgata gctatgaaag gcttctcgga aagcttggcg tttgttgagt
     8221 accgtttccc aagacaggcc tgcctgatag gtttccatac acaacaactc aaacaacgct
     8281 tggtcatcat ggaggggctg gccccactcc tcatcatgat aggcgatgta gagtgggttg
     8341 gtcatcttga cccacgaaca acgttttgtc attctctgct cctatttgac caacatttta
     8401 agggccgact tgatatagtt ctcagctgta tctgtcgttc cttcaaagaa tttcttgatt
     8461 ttcttgagct ctgttgcctt gtagcccaga gccaacatgg cttccatagc ttcttccaat
     8521 tcttggtttt cagcacttgc ttgcactgcg atcttggcag gaaggtcatc tcctgcaact
     8581 actaccttgc cttccaagtc cagcaccatc tgctgggctg ttttcttgcc aattttaggg
     8641 aacttggtca agtaggtgat gttcttggtt tcaatggctt gaaccaagcc agcattgtca
     8701 tcagcagcga taatagcaag agctgataca ggaccaatcc cagagaccga aatcagacta
     8761 agaaagagct ttttctcatc ctctgagcga aatccataaa gcaaatgggc gtcctcacgc
     8821 acaacctgat gcacataaat ctgagcctcc tgattaacct gacctgaata ggcataagga
     8881 ttggccacat gcaggatata accaatacca ttggtttcaa gaacaatgta tttggcagta
     8941 attttggtaa tgattccttt taaatatgcg tacatagtgt tccttctctc ttatttcgtt
     9001 tatctagtat atcatatttt ctttatccga ggagcgagaa tcgattatca caaatacagt
     9061 atatcaaaaa caatcggtat ttatctgttg acatattcaa aattaagtac tataatcatc
     9121 tgtaattgaa taactatctt cagggcaggg tgcaattccc taccggtggt atagcccacg
     9181 agccgaaagg catgatttgg tgaaattcca aagccgacag tatagtctgg atgaaagaag
     9241 ataaaagtat atttgtgctt tttgcgtgct ctgaaatgat tacttgtcat ttcagagcat
     9301 ttttgttaat cgcataagtt ttcttttgta tgccttgagt agtctcctat tcaaggtata
     9361 tttttttgga ggtagtgaga tgagcgattc aaaatatatg aaattagcaa taaaactggc
     9421 acaaaaaggg gctggttacg tcaatcccaa tcctatggtc ggcgcagtta ttgtaaaaga
     9481 taatcacatc atcggacaag gttatcatga gttttttggt ggctcacatg ctgagagaaa
     9541 tgctcttaaa aactgtagag aatcccctgt cggagcgacg ctttatgtaa cacttgaacc
     9601 ctgttgtcac ttcgggaaaa cacctccttg tatagatgct ataatcgata gtggtattac
     9661 aagagtagtc attggaagcc tagactgtaa tcctattgta tctggaaaag gagtaaagat
     9721 acttgaggaa aataatcttc aagttactgt tggaatttta gaaaatgagt gtcttaactt
     9781 aataaaaagt tttagaaagt atattaccca gcatgtaccc tatgttttta tgaaatatgc
     9841 aatgacaatg gatggaaaaa tagccactaa aacaaatcaa tccaaatgga ttactgaaga
     9901 agaggcaaga aagcatgtgc atcagttacg acaccatgtt agtgcaatta tggtgggagt
     9961 caatactgtt attcaagacg atcctttgct gacatgtaga ttggaggaag ggaaaaatcc
    10021 tatccgtatc atatgcgata cacatttacg aactcctctt acctctaaaa tcgtaaaaac
    10081 agcaaatgat attaaaactt acattgccac ttcctctgaa gacaaaaata aaatgaagct
    10141 atatcaaaat catggctgtg aaatactttc cataaagaaa aaaggcaatc atatagactt
    10201 atcgagttta atgcaacatc taggaaacat gcagattgat agcctagttc tagaaggggg
    10261 cagtctaatg aattggagtg ctttggaaca acaaattgtt gatgagctga aaatatatat
    10321 tgcaccaaaa atttttggag gcagtgccaa gtttcctgtc ggaggtgaag gcatttcttt
    10381 gccaaatgac gctattagat tgaaacctta tgctttttct caaataggaa atgactatct
    10441 catagaaagt gaagtgattt atccatgttc acaggaataa ttgaagaaat cggaaaagtt
    10501 gaaagaatac agaaagactc tcgtaattgt aaactatcaa ttaaagcctc aaaaatatta
    10561 acggatatcc atttaggcga tagtatagca gtaaatggta tctgtcttac agttactcat
    10621 ttcaatcatc aatcctttac agttgatgta atgaatgaaa catggagtcg aacagctctt
    10681 actctattaa aacatggaag tgaggtgaat ctagaaagag ccttatctgt caacggtcga
    10741 cttgggggtc acgtcgttac aggacacatt gatggtacag gaaaaatctc gtcaataaaa
    10801 aaagatgcta atgctgtatg gtatcaaatc aacacacaaa aagaaatttt agatttaata
    10861 gttgaaaaag gatctattac aattgacggc attagtctga ctgtcgctaa agtctccaaa
    10921 gtaaactttt cagtatctgt tatccctcat accttggaac aaaccattct taagagtaaa
    10981 caagtcggga gtacagtaaa tcttgaaaat gatatcttag gtaaatatgt gcaaaaactg
    11041 atggataact ctccaaaatc agaaatatct aaggaactat tatatcaaaa tggattttag
    11101 cagaaaggat aatcagccaa tggaatatcg aaaaatacaa gaagcattag aagcattgca
    11161 gaagggacga cttgttcttg ttatagacga caaggataga gaaaatgaag gagacttaat
    11221 ttgttctgca caagcagcta caacagaaaa tgttaatttt atggctactt atgccaaagg
    11281 attaatttgt atgcctatga gcgaaagttt agctaatcaa ttaatgcttt cacctatggt
    11341 tgaaaacaat acagataatc ataagactgc ttttacagtt tcaattgatt ataaagaaac
    11401 gaccacaggt atttctgccg aggagagagg actgaccgca cgtatgtgtg tagctgaaga
    11461 tataacaccc tctgattttc gcaggccagg acacatgttt cctttaattg caaaaaaagg
    11521 tggtgttcta gaaagaaatg gacacacaga agcaactgtt gatttattaa aattagctgg
    11581 actaaaagag tgtggcctat gttgtgaaat aatgaatcat gatggcaaaa tgatgagaac
    11641 agatgattta attcagttct cgaagaaaca caacattcca ctaattacca tcaaagaatt
    11701 acaagaatat agaaaagtat atgatcagct ggtagaacga gtttcaactg tcaatatgcc
    11761 tactagatac ggtaatttca aagcaattag ctatatagat aaactaaatg gggaacatca
    11821 tcttgctctt attatgggaa acatagagga tgaagccaat gtattatgtc gggtccactc
    11881 cgaatgttta acaggggatg ttttaggctc tttacgttgc gattgtggac agcaattcga
    11941 taaagctatg aaaatgattg ttgagaatgg ttcgggtgtc ttactttact tgcgacagga
    12001 gggacgagga attggactta tcaataaatt aaaagcctat catttacaag atcaaggcat
    12061 ggatacgctt gatgccaatc ttgcattagg ctttgaaggt gatttaagag aatatcatat
    12121 tggagcacaa atgcttaaag atctgggact tcagtcactt catttactga caaataatcc
    12181 tgacaaggtt gaacagttag aaaaatatag aattaccatt tccagtagaa tatcaatcga
    12241 aatagaagcc aatccttacg atagttttta tttagaaaca aagaaaaatc gaatgggtca
    12301 cattttaaat atggaggaaa aataaatgaa cacttatgaa ggtaatttag tagcaaacaa
    12361 tattaaaata ggtattgttg tagcgagatt taatgaattt ataacttcaa aattattatc
    12421 tggagcacta gataatctca aaagagagaa tgtaaacgag aaagatatag aggtagcctg
    12481 ggttccagga gcttttgaaa taccactgat tgcatcaaaa atggcaaaaa gtaaaaaata
    12541 tgatgcaatt atctgcttgg gagctgtcat tagagggaat acaagtcatt atgattatgt
    12601 atgtagcgag gtatctaaag gaatcgccca aatcagttta aatagcgaaa ttcctgttat
    12661 gtttggtgtg ctaacgacag atacaattga acaagccata gaacgagctg gtactaaagc
    12721 aggaaataag ggttctgagt gtgcacaagg agctattgaa atggtcaacc taattcgtac
    12781 attagacgca tagaacagac tctactttta gacgaataaa gaaacttggc aaaatcgcaa
    12841 tttaatactc tgtgaaaatc atagagcaaa ctaggaaacc agtcgcagag tggtaaaaca
    12901 caacttcgat gttacagaca aaactggcaa aattagctca aaacactgtt ttgagattac
    12961 tgataaaact gacgtcgttt aaaaagagtt tcgaaaagaa ttaggaaaaa atgaagtaac
    13021 tatttccatg ataaaacgca taaaatcaag gtttttgaac acctgatttt atgcgttttt
    13081 ctgattttga aaatttttcc cagacttttt tatactttta atatttcccc aactcacgga
    13141 gactggtgtg attttcctga atacgtcgga acatcttttc catatccgac ttggtaaaat
    13201 gcaccaaaac aggacgtccg tgaggacagt tatagggatt gtcacattga gaaagctgat
    13261 agaggagttg tctagctgaa tgatcatcaa tacgatgatt ggccttgata gatcgcttgc
    13321 aagacatcat gatagccagc tctgctcggt atttcttgat agaaacttcc ttggtcaaaa
    13381 ggagcatgtc gcacatctca tagatgcctg attcaatctc ttcttctgcc atccaaatag
    13441 gatgttcacg tagaataaat tgattttctc cgtactctgc tagaaagacg cccacttcct
    13501 ctaagagagg cattctttcc ttgagacgca gggcatcatc cgcaggaaat tcaaagatat
    13561 agggcactag gagttgctgc tggctctggt caacattgcc aatgctttca cggtactcct
    13621 cgtacttgac ccgttcctga gcagcgtgct gatctatgat gtaaagtcca tctcgccctt
    13681 gggcaaagag ataagtcccg tgcatttgtc cgaaaaactc caactctggg aagctggatg
    13741 cttcttctcg ctccagtttg tcataagcct tatcgatgct agcaagatct aactctggat
    13801 ggtctagctg gtcgtagtta gcaggctttc tctctgcaaa atgcagtttt gctggcttgg
    13861 tcaatcggtc caaggtttcc ttggcaaaca gggtcaaatc ctgcccttca tcagtcaatt
    13921 ctacctgata atcagctact tcagtttgac taggtcttga cggctcagtt ttctcatagt
    13981 agagcgtatt ttctttgagt gggagaatag tttgctccac cttttcacga ttgcgcacgg
    14041 tcgatttggc aagattttcc aaggcatctg gaatcaaggt ttgttccttg agactatttg
    14101 caatagcttc tgaaaccaga gtcatcagtt ctttttcctt ggaaatccgc acctcttgct
    14161 tagttggatg cacattgaca tccgctagat aagggtcgat atggatgtga atgacagcca
    14221 gtggaaaacg tccaaccata agcttgcttc cgaaaccatc caaaatagca cgattgagca
    14281 ggaagttctt gatataacgg ccattgatga agaggctgat ataattgcgg ttagcccgag
    14341 tcaactcagg caaggacaca aaacctgaaa tttcgaaatc taggtcagag ttctcaattt
    14401 caatcatctt cttggcactc gccaaaccgt aaatccctgc gattgcttgg cgcagttgac
    14461 cagtccctgc tgtccgcgtc atttccttgc catcactaat caagctaaaa gaaatctcag
    14521 gatgggccaa gcccagacgg ttgacaatat caatgatatg agacaactcc gcttgctggc
    14581 tcttcatata cttgagacgg gcaggcgtgt tgaaaaagag atcctccaca caaaccttgg
    14641 ttcccacagg actagtcgct gggatgactt cctcaacttc acccccacgt gcgactaact
    14701 tggttccatg actagcacca tccaccgccg ttaacagagt caagacacta acagacgcaa
    14761 tagaaggcaa ggcttcacca cgaaaaccaa gcgtccgaat ccgaaagaga tctgcttgat
    14821 tttttatctt actggtcgca tggcgacgca gggccaactc cacctcatcg tgggcaattc
    14881 catgaccgtt atccgtgatt tgaaccttct tgagaccagc ttcctcaatc tcaatgataa
    14941 tctgactaga gcccgcatca atggcatttt ctaccaactc tttgaccaca ctggcaggac
    15001 gttcaatgac ctctccagcc gcgatttggt ttgccagcat ctctggcaat tcaataatat
    15061 gagacatctt tcagcccctt tccgtatcta ttttcctaga aattgattag tgctattata
    15121 ccatatttca gaaagaacag aaaagctcca ccacatagga gcccaatctc tccacatagg
    15181 caaagtccct tgccaagggg cataaaatag tggtatactc aataaaaatc aaagagcaaa
    15241 ctagaaagct aggcgcagac agttcaaaac actgttttga ggttgtagat aaaactgaca
    15301 aagtcaacaa ctatcctatg gcaagacgac gctgacgtgg tttgaagaga tttttgaaga
    15361 gtattaataa aggtgtacaa gaagtgagat taattttgta tgaaaaacaa ggagagaaat
    15421 ttatgaaagt agaactacag attagtgaga cttacgagga ggaaaagctg attgtccaag
    15481 cacctcagcc gacagataaa gtccagaaag tcatcgagtt cgcagaaaat ctggaccaaa
    15541 gagaaaaaat caaagggaaa atcgatgatc aggtctatct agttgagatt ggcaagattc
    15601 agcgcttcta tatcgagaat cggaaggttc tagcagaaac cgcgagtcag acctacagca
    15661 ttgatttgcg actctatcag gttcttaaac tcttgccaac caattttatc caaatttccc
    15721 aatcagaaat catcaatatc gattccatct ctcacctcaa gctcacgcca aacggtctgg
    15781 tagaaatttt cttgaaaaac gaaagcttca cctactcttc acgccgttat ctaaaaacca
    15841 tcaaggagaa attagaacta tgaaaaaaca agtatttcac gatgcagcta ccggtgttct
    15901 tatcggcctc atcctctcta tcctcttttc actcatttat gcaccaaata cctacgcacc
    15961 actaaatccc tactctctca tcggccaagt gatggatcag catcaggttc acggtgccct
    16021 ggtcttgctc tactgcacac ttatctgggc aaccatcggt atgctcttca actttggcaa
    16081 ccgcttattt agccgtgact ggagcatgct tcgtgccact ctgactcatt tcttccttat
    16141 gctggctggc tttgtcccac tagcaactct tgctggttgg ttccctttcc actggatttt
    16201 ctacctccag ctcattatcg agtttgcgat tgtctatctc atcatctggg ctattctcta
    16261 taaaagagag gctaaaaaag tagatcacat caatcaactc ttggagcata gaaaatagga
    16321 acctcataat aagcaaatcc gagaaagaaa cttctcggtt tttctttatc ctgctgcacc
    16381 ttactatagt gaaatgaaat aagaacagga taaatcgatc aggacagtca aatcgatttc
    16441 taacaatgtt ttagaagcag aggtgtacta ttctagtttc aatcctctat atcataacag
    16501 ttatttcaga tcttctttgt aaattttcta tcttttttca aaatactctt gcatttttcc
    16561 aaaatttttt gtagaatata gattacatat acagattatt ctgaaaattc aaaaaggagt
    16621 atttatcatg tcgcaagcta tttccttaaa tcaatcaacc tgggcaagca agctaaaagc
    16681 aatgggacct ggaatcctaa tggcaactgc cgctgttgga ggttcccaca ttgtatcctc
    16741 aactcaagct ggcggttctt acggttggtc tctacttctc ttggtcatct tagccaatgt
    16801 ctttaaatat ccatttttcc gttttggtgc tgaatacaca gctgatactg gaaagacttt
    16861 ggttgaaggt tatgccgaaa aaggaaaact ctatctctgg attttcttta tcctcaatgt
    16921 cttttcggct atggtcaaca cggctggtgt tgccattctg tgctcagcta tcatcgccag
    16981 tgccttccca atgattggac ttagtattac tcagtggtcc ctcattctcg ttgcaatcat
    17041 ttgggctatg ctactctttg gaggctacaa acttttagac ggcatggtca aatggattat
    17101 gtctgcctta accattgcga ctgttctggc agttatcatt gcggcggtca agcatccaga
    17161 atacagttct gattttgtcg agaagacacc ttggcaaatg gcagctctgc ccttcatcgt
    17221 ctccctccta ggatggatgc cggctcctat tgaaatttca gccatcaatt cactttggtc
    17281 agctgaaaag agaaagaccg tcaactttaa cacagaagac gctctgtttg actttaacac
    17341 tggttatatt ggaacagcta tcctagccgt cttctttgtg gcactgggag cactgattca
    17401 gtatcctaca gggcaggcgg ttgaagctgc ttcagccaaa tacatctctc aattcgtggg
    17461 catgtatgcc tctgttcttg gcgaatggtc ccgttacttg attaccttta ttgccttcct
    17521 ctgtatcttt ggaacagtta taactgttat cgatggctat tctcgcgtta atcaagaatc
    17581 tctccgactg ctaatcagtc aaaaagagga caatcgtaaa tctttgaaca tctggatgac
    17641 catcactgct atcatcggta tcgtcattat caagttcttc gctggtcagg tttcaaccat
    17701 gctccgcttt gccatgattg gatctttcct gacaacacct ttctttgctc ttttgaatta
    17761 cgccttggta acgcgtgaaa acaaaaatct tccttcttgg ctcaaacacc ttgccattgc
    17821 gggattgatt ttcctctttg gcttcgccat cttctttatc tacgcactcg caatcggaaa
    17881 agcagggtaa gggacaaccg cgagatgaag ataaggtttc atttcaagag aaaattcagc
    17941 aaatatttct atgataaaaa gcataagaac aaggttttga agacctgaac ttatgctttt
    18001 ttacgttctt aaagactgtt tatactcaaa aaacagttga acaacttcaa ccacctctta
    18061 taagaacttt atactattcg agaatctctt caaaccacgt cagctctatc tgcaacctca
    18121 aagctgtgct ttgagcaacc tgcgactagc ttcctagttt gctctttgat tttcattgag
    18181 tattaattct ccttttccaa ctcatacaaa tctgcgataa tagctgcgac atgtttgata
    18241 tcttccagca tgcctcgcat ttcaaagtca gccaatacag ggaagccaaa gcgttgactg
    18301 tattgcttgg ctgttaggca gtattggtta ttaaagttac gatttcctga cccaaccaca
    18361 ccaaaacact tactagcatt gttaccatag gcaataaaat ctcccaccgg tgtcgtcaaa
    18421 atctcaacat ctccgttatc cacgccattc ccaccttcga gataggtcgg caaaaaagcg
    18481 acatagggat ggtccatttc atagaaattt ttgccttcct tgaccaaatc cttgatatga
    18541 atcttttgaa cctcaatccc tttgtactgg gacaagagat agtctttcaa gcgcgtcaca
    18601 aaactttcag tgttgccact caaactaata taaactaaag aaattgtctt catatgtacc
    18661 tccatgattt tatctataat caaaaaacaa ctaccaagat tgtatcttga tagttctttt
    18721 ttgtcaatac ttaggcaagc tcttctgttt cctcttcttc tgccaaggct ttctgttgtc
    18781 gccacatctt atagaagaca agggctagga agagaacatt gatgacgata tagaaaatgc
    18841 taacagcttt tgaaccaata ctcactgtca aacgcatgct ctcatcttga accaactgca
    18901 cagcgtcttg taaaccaaca tagccataaa tcgaaccaat aatagctaac aaaacatagc
    18961 caacgtaagt atagtttgca tattgcaaat ttttacgaac aaggaagaca atcgctacga
    19021 ccactaaaat agtagatagc acaatcaaag caacattaaa aatagaatga gatgcttctg
    19081 ctactctata ggtataatta agctggtctt ctaattgctg agcattgacc cctgcaactt
    19141 gtttctgagc agcacgaagg gcttctttac taggcaaggg actcacaatt ccaaacagag
    19201 acggaactga aataagggca gacaagatta gcaagaccca tagataaatt ggtttctttt
    19261 tcatgataga acctcctgat aatattatag caccttttgt aaaggaatac aaattttata
    19321 gtatagtttc tacctgtttt cggtaggctt ttggcgattt tccgcacaat tttcggaaaa
    19381 ctttatagaa atatccaagg ttactgtaac caacagcata acaaatatct tcgatgctgt
    19441 cactagttga aagcaaaagt tgttgggcag ccttaatgcg ttgtttgttt agtaactctg
    19501 caaaagtcga atcggtttca cgcttaatca actgccctag atagacagga ttgataaaga
    19561 gagccttact gatatccttg agggaaagct ctttttggta atcacgacca atgacttcca
    19621 gcacactgac cacattttca ttcatacggt attgaccgaa aaagctgctc agagtttcct
    19681 tgatataagg aaccaattca tcgaaggatt gaatagcatg aatggttttg acgatgtctg
    19741 tcaggtcatc agctttgaga tgttcaaata aatggaaaac atccatgaca aactgaataa
    19801 aaagctgttt agtaatcgga acacgcggcg tattttcaag aactaccttc tccaagagat
    19861 ttaactcttc tacgatttga ttgagatttc cctgaatgat aaccctataa atcggttcgt
    19921 aataggcaaa gagactctca gactgctgca gatttacaga accgtaaaag agggttttct
    19981 cagcattcag cttccagcgt tcaaagtgtt cttgataagg agcttcaaaa ggtgtaacga
    20041 ctaaaccatc taggggttgg tcagaaatga aaatctgcca gtcttgaccc aagacatagt
    20101 agggaatggt gaaggaacct tgtttttcct tggatagacc tatccaccaa ttctccttat
    20161 cccctaaata actaacaaat ccagcctcgt ctaattcttg actaagagtc tgacttttct
    20221 tccctctctc gccgagctga cctgcaatct tctccagcag atttcccagc tctaccttat
    20281 caacaggctt gaccaaatag tccaccacac taaggttcat tgctcttttt acataatcaa
    20341 actcctgata acctgagagc aggatatagg cagcatctgg taagatctct ttcatctccc
    20401 gaatcatatc aagccctgtt ttgtctggca tattgacatc ggaaatgatg acatcaacag
    20461 gattttcctg aacatattct agagcttcat cggcatgact ggctgttgcg acgacctcca
    20521 tatcccactt atcaaaggga atcaaacgct tcagaccttc tgtcaccatg tactcatcat
    20581 ctactaataa tactttatac attttctccc tttctactca tcttgaattg taatacgata
    20641 ctgaacaccg gcttgctctg cagactctat cgtaatggca tagcggtctc caaaatagag
    20701 cacaaaacgc tcgtgtacat tgacaatccc gatagactgc ctttgatcac tgtagctggc
    20761 ttggtgttca aaatatctct gacttaattt ttctcggata tttgccaact tttcagccga
    20821 cattcctcta ccattatcga ccaccaaaat ttccacaaac ccatcctgtt taagagcctt
    20881 gatgctaatc acattatctg tccgcctgtg gtcaacacca tgcgcgaaat agttttctac
    20941 cagcggttgc aaggtaaact tgggaatctt catattctct aactctggat ctatcttgaa
    21001 accataggca atggacttgg gatagcgaac catgcagaga tagctgtatt tacggcaaaa
    21061 ttctaattcc tgtttgagga gggtctctct ttcgtcggaa atattgttac gcaagagact
    21121 actgaattca taaatgatat ctgccaactc atcttgactc tgcatgactg cgtacatacg
    21181 caagaactcc agcgtattat acatgaagtg agggttgatt tgcgcctgca aggcccgcat
    21241 attggcatct ttttgactga gttctaactg gtaaatatca tggatattct tttccaatcg
    21301 atccaacata tcattggtcg tctccgcgat taggagcaat tcctgatcct tttcaagcgt
    21361 atcaatgcga agaccttctt gtccttgggc aatagcttgg atggaatcca ccaaatccac
    21421 tacctgcttt tgataattag caaaggtctg cctcaaagtc agataaagaa tgacaataaa
    21481 gagaaggctc aagcccacaa tcaaagcaga gctggtcacc gttccttgta aaacaaagtt
    21541 tttgggaact gccacctgaa cctgataacc atgagaagtt aagccaacta gccaattttc
    21601 actctcctta tcaactgtct caccaatatg aaaaatctcc gtatcaaaag gtgaggtcac
    21661 tgttactgcc atcggagtat gacctctagt attatcaatg gcatggtata aaacagcagg
    21721 atccaaggag atgtaaatca ctcctaagtc ttgatctgac actggatctg acaccggaat
    21781 ggcaaaacta tttcctgctg gtttgaaatc ctcagccctg attttttctc cactacgttt
    21841 gtctttagta gaaacataga cttccttgta atcttgaaaa gcaatggcca ctccagttac
    21901 aaaatcattt cgaacataga ggtcatcaac attttcatac agagagactg atatataagg
    21961 cgaagctttc cgctctaatt gccagtaaaa atagtctggc atgctaaggc taaagtattt
    22021 atagattccc tcaatacgag cttggttttc aactaaagcc tgagctatct gggttgactc
    22081 tctatggtaa tattcaattt cactcactgt tctagcagtt acacgctggg ctactctctg
    22141 ggcttccttt tcacgtgaat cccagtcagc gtaagagagt aagattgcaa aactcgcaat
    22201 gataatgatc atcactgaac tataggttct tagaagcgta tgtagccaaa agtgcttcat
    22261 tctattttgt cgccaatttt tcatggatga tttcatagac aggttccaac atatcactga
    22321 taaagaaatg ccacatccca attcctacaa aaaatagcag ggcaggcata tagtaaccaa
    22381 ttgctacaag tagcacagtc ccaaggagaa ccttggccac agctgctaga ctcataaaga
    22441 tgccaatcaa ggataatttg agactatttc gataggacaa ggcaaaataa acttgtaatt
    22501 ttaaagatac tgtataagcc aaaaagatca gggcaactac taggacattc aaaagggtcg
    22561 caatcaaatg aacaatggtc tgatgaggca attgcaccaa tagatacaaa ccataggtca
    22621 aaactagacc cacacctaaa aagctataga aaatcaggtt gcttgagaca aaattttgct
    22681 tgtaaagaga ccaagcctcc ttcaaactgt attcccgaaa gctataacca tgttctgcat
    22741 ataagctcat caaggtggca ctagctggcg ctagaccaag gataatccct cctactaaag
    22801 ttaatagcca aaagaaggct gtcgcaatca tccctaaaaa gaaacgatta aagacaaagt
    22861 ctaaaaatct acccatgata ttcccctaaa aataactatg aaaccattat atcatatttc
    22921 aagcgttttc aaaaacttct agtatccatt tacaatccca ccaaaccgtg gtacaatgag
    22981 aattgtgaaa aaattatcag gagacaattc atgaagaaat ctatcttaac tacactgctt
    23041 tttgcagttc tttacttcct ctgcatgggg attggtgtcc ttttgggcaa tctctttgac
    23101 caaactggaa acatgtttta tgcgcctgcc tttactgccc ttgtcggcgg tagcgtctat
    23161 atgatcctag tcgcaaaagt tccgcacttt ggagccatta ccactatcgg ccttgtcatt
    23221 gccctctttt tcttgggaac taaacacggt gctggttcct tccttcctgg aattatctgt
    23281 ggcctcctag cagatggagt agctcattta ggaaaataca aggacaaaac aaagaacttc
    23341 ctttctttca ttattttcgc ctttagtaca acaggaccaa tcttgcttat gtggattgcg
    23401 cccaaagcct atatggctac tcttctagca agaggaaaat cccaagaata tatcgaccgt
    23461 atcatggtcg ctccaaaccc tggaactgtc cttctattta tcgcaagtat tgtcatcgga
    23521 gccctagtgg gtgccttgat tggacaagcc ttgagtaaaa aatttgcaca gaaaatctga
    23581 tcagttaaaa agagccacgc ggctcttttt atttatgact caatttctta gtcaagaaat
    23641 ctcccaagaa ttggattgca aagataatca aaatgataat aatggttgcc aagatggtca
    23701 catcgtgatt gtagcggtta aatccataag cgatggctac gttaccgata ccaccagctc
    23761 caaccgcacc ggccatagct gtttccccaa caagggaaat caaggtcaca gtcgtcacac
    23821 ggatcaaatc tggaagacct tctgataggt aaacacccac gatgtcccag aaagtcgctc
    23881 cgctcgcttg agccgcctca atgacaccgc catccaattc agccaagaca acctgcacct
    23941 gacgggcaaa gaaggcaaag actgcaaaag aaagtgggac aagggctgca tttggcccga
    24001 tacttgtttt aacaatcaag tgagaaagtg gtgacaagat tgccaagagg atgataaagg
    24061 gaaccgcacg aaaaattgag gtaattttgt ctaaaatcca gaatacgact ttattctcca
    24121 agacaccacc tggcgctgtc aagacaagga aaagacctgc cactagcccc aagaagcctc
    24181 cgataatgaa ggaaagaact gtcatataaa gagttaagta gatagccgtt ccccagcctg
    24241 cctgaccagc ccaacccatt ttatagacat ttggtaaata ggtttgaatc aatgattcca
    24301 tcttactgta ctcccttcaa tacttttagt tgtacacctg cttgacgaat ggcttcttgg
    24361 gcacctgcca acgctgcttt ttcacctgac aagaccacca ccaattctcc aacaggagta
    24421 ccatcgagaa tttcgatatt cccatagaga atattagcca ttacttggta atgcttgtac
    24481 aattcattca aaagtggctc gtctgttgaa gctccagcgt acttgagttg caccaagaga
    24541 ctgttttcag acaagtgttc cacgatttct tgcttctcga ttttgaccat ggcttcgtca
    24601 atacctgtgg ctgttgagat aaagtcttga gtcaaaggtt gtttagggtt tgagaagatt
    24661 tcaaggacac ttccctcttc aatcaaatgc ccatcctgca taaccgccac acggttggca
    24721 atgtctttga caatctgcat ttcatgcgta atcaagacaa ctgtcaatcc taatttttgg
    24781 ttcaaatctt gcaacaaggc caaaatctgc ttggttgtct tagggtcaag ggcagaagtt
    24841 gactcgtctg aaatcaagat ttttggatca ttggccaagg cacgcgcaat tgccacacgc
    24901 tgtttttgcc ctccagataa ttgtgaaggg tagttttcag cacgatctgc caaaccaacc
    24961 aagtccaaca acttagctac tttagccttc ttttcttcct tgctgagtcc agagtgttta
    25021 agggcaaagg ctacattctc ctctgctgtc ttttggctca tcaggttaaa atgctggaaa
    25081 atcatcccga tatcttgacg tttacgacgc aactgctctg ccgtcaaggt gaccttacca
    25141 tcaaaaatca catcgtcgtc aatggtaatt ttccctgcag atggtttttg caagaggtta
    25201 atcacccgta caagggttga ttttcctgct ccagaatatc caacgattcc gtagatatcc
    25261 ccttcttgga tgtgaatggt cacatcctta accgctgtaa tggttctctt cttttggtga
    25321 aaagtcacat cgatctgatc taacttgata atatctctac tcatagcttc taatcagctc
    25381 ctctactaat tcgatatggg tgtaataatc agcgattcgc acattttcat ctccaccgtg
    25441 gtctcggcta ttggcatttc ctagaccgaa tgcaaccatt ggtacctcta gggcatcaaa
    25501 gaccgtatgc ataggtcctg tccccgctgt cgtcggcaag actgaaacgc cctgtggata
    25561 gaatttcttg gccaactcga tcacattgag aatggctggt gcgctcatat cgcttcgata
    25621 gctcatctct cccaaggtat agtataattc taccttatca aagccatttt tgtctagttg
    25681 tttccgaatt ttttccagaa catcatgcgg ttctaggccc ggaaccagac gaacctctag
    25741 cttggcactg gcttctgcag gtaaaatagt cttaacaccc tgaccttgat aaccagactg
    25801 gattccttcg atattaagcg ctggctcgaa aaagaaacgt tttagaaagg ccatccgctc
    25861 ctcctgtaag agaggcaact ccaatccata aatccgacta acttcctctg ggtttcgttg
    25921 accataagtt tctagcaagg ccatttctcg ttcattgggc tcttgtactt cttcgtacaa
    25981 gccttcaacc aagatacggc catccgcagc acgaagagac tgtaaggctt ggaggagata
    26041 ccaaggagct gattccacaa caccaccata actcgagtgg atatccacat cagcgctttt
    26101 taccttggca tcaaaggtca caattccctt attgccacca gaaatttcca gctgttccaa
    26161 ggcatttttg gtcccttgtt cccagaccaa caaatccgcc ccacggagtt tgtctgcatg
    26221 cttttccaaa tacttatcta ggtctgttga agccgattcc tccgctccct ccatgataaa
    26281 gctgatattg acaggtaaat catcatggtg ctgcatatat tttctcaaag cactcaagcg
    26341 agctgtgata tgacccttgt cgtcatcaac cccacgccca tacatgaagc cattgcggac
    26401 cgaaagcgta aaaggatcct ctgtccagac ctgatcccca tccgctggca cagtgtcata
    26461 gtggttatag aaaatcaagg tcttggcatc tggacgcgaa ctcttgaaat gtgccatgac
    26521 aaagggtgct gtatagctct catcaatctc cacttcagct ccaacacgct tgaaaatctc
    26581 acccagataa tttgcgactt ccttgagtcc aacctgctgg gcaaagactg atttcttaga
    26641 aatcaaggta cgcaaaacct caaaataatg ttggactaca tgatcctttt caaatttttc
    26701 aatctgttct tgttcgctag gaaaaaccat attctctcta cctttctatg aacgaatagt
    26761 cttctttaca atctatactc tatacaaaag caagaggtgg aattttctct cccacctccc
    26821 tgtttcttat taccaaactg gttgatccaa accatctgat gattcttcga taactttttt
    26881 cacgtcatct gtgtggtaag ctgcgattac tttcttgata gcatcagcct taggtgatgt
    26941 ttcccaatct ttttttgcaa caatgatgtt gtaccattgt tttgagtttt catcagcttg
    27001 ttctttgaaa agtgctttct tgtagtccaa ttttgcttct gtaacgaagg tattgtttac
    27061 aacggcagcg tcaactgatg acaatgaacg agctgtttgg ctagcgtcca attcagtgat
    27121 tttcaagttc tttggatttt ctttgatgtt ggcaactgtt gcaagagcag ttccagaaac
    27181 atccaattta atcaagccag ctgattgaag caaataaagc gcacggcttt cgtttgtagc
    27241 gtcattcggt acagcgattt ctccgtttgc tgggatgtct tctactttag tgtacttgtt
    27301 ggcacttcca ttcaaacctg aataaaggcg gattggagag atgtaagtat ctgcaatcgc
    27361 tacaagatct tttccgtttt ctttgttcca gttattcaag aagttatagt gttggaaagc
    27421 gttcaaatct acttcgccat cagcagttgc tttgtttggt tgtgagtagt ctgtgaactc
    27481 tgtaaattcc aaggtaattc cgtctttttt aaccaattct tggattttgt cccaacgttt
    27541 ttcttcagaa ccgctacggt taacagttgc gattttgata gttgttgcat tgtctgcttt
    27601 cttttctgag tttccgcaag ctgcaagagc caaacctgcg actgtagcaa gggctgctag
    27661 gccaagccat tttttgattt tcatgattct ttctccttaa aaataatacc gtactagtat
    27721 ctcacaattt gtttgatttc acaaattgtt attagatagt cagatatata gtttaaaact
    27781 atatccctaa aaactgagaa accacccacc ttactcagaa tggatgattt caagaaatta
    27841 tttaatatca gcttctgccg gtaggtaagt gtctccgaag aattgtttag acaatttttc
    27901 aagagttcca tctttataaa ggtctttgat gcgtttgtct acaaatgatt tcaactcatc
    27961 ttgaccttga gcaagaagtg ggtaaacgta cggttgttgg tcgcttggaa gttcgataac
    28021 tttcaagttg tccaaacctt ggttcttgat cactgtttca acaccgattt tatcaaaaat
    28081 cttatagtca aattgtccat cgctcaaacg taccatgatt tgttggaaat ctgccttagt
    28141 atagttaagg atagttgggt tgtccgtgtg ttcagcgttg tatgcttcta actgcttagc
    28201 tgatgtagtg gcttgaacga cttccgtcga ttttccaccg atatcatcga gagacttgat
    28261 actagagtca tctttcttca cgacaaggac attaggattt tgggcaattg gtgcggcata
    28321 gaggtatttc tccgcacgtt ctttagtgta gctaagattg ttgacagcca tattgtaacg
    28381 atcagcgtca agaccagcaa agacacctga ccattctgtc ttttcaaact tgacatcata
    28441 tttgtcagaa tctttaaaga tagcgcgaac gacttcaatc tcgtaaccag tcaattcgcc
    28501 attttcttca tagataaatg gccttggtga tccattggtt gcaacgatga tttctttctt
    28561 gctagctgct tctccttctt tcttagcacc ccctgagcaa gccgcaagca cacctgcagc
    28621 aacaagtcct agggcagcaa gagatgagta tttaacgatt tttttcatgt catttcctcc
    28681 aaaatagaat accttataat cttaacagaa aaagaccatt tacgccatta tatgatatct
    28741 atctctgtga taagttttct ttatagttga tttaaaaggc caaacgcaag atggcaatca
    28801 agactactcc aaagagaact gttccgacta gattgcgata gcgaaaggcc acccaagctg
    28861 ttggaaagac ggctaagaag tccagccatt tgatttgagg aagactgcca accttgcctg
    28921 tcactacgct tgaaagaatc aaggcaaaga taatggaaac gggcaagaac ttcaaaaaac
    28981 gctcaacgat tgcaggcaag cccttatact tgactaagat gaagggaatc atacggggaa
    29041 tccaagtcac caagccagag aaaataactg ctaataaaag atacttactg accatctaaa
    29101 accaccccca tgctacaacc aagtagcgtc gcaaacagaa tagctagtga ctgagacacc
    29161 actgtcaaga gcaaaaagaa ggacaccgca acaactgcta ggataatgag cagattgcgg
    29221 acaggaatcc gtctttgcat aatctgaaat tgcgaagcaa aaataccaat aaacatccca
    29281 accagggcaa aatccaagcc aaagatttct ggatttggta gcaggccacc cagagccgtt
    29341 ccgactactg tccccacaaa ccaagccaca tagctgttaa gattgtttcc gtgcatccac
    29401 ataggattta ccttgtctgt atgggccaat tcacccatca aaacgccata ggtctcatct
    29461 gtcaagatac tagacatacc gatattgtac caaagactgg tatgacggaa ataggtcgac
    29521 gcatgtaagc tcagcaaaaa gagacgcaaa ttaatcaaaa agaccgtcat agcaatagct
    29581 gccacaggcg ctttaaccgc aatcagtgcc agcatggcaa actgggcact cccagcataa
    29641 acaaagagac tcatcaagcc catctcaaca agtgtcacat agggcgcacc gataattcca
    29701 caggccaggc cgatactgac atagccaaga gccgttggca tggctgcctg cgccccctcc
    29761 taaaatcctt tttctttcat ctttctcctc atattgtctt aataatactc aatgaaaatc
    29821 aaagagcaaa ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgtggat
    29881 agaactgacg aagtcagtaa ccatacctac ggcaaagtga agctgacgtg gtttgaagag
    29941 agtttcgaag agtacaagta ggctgaaaag aatccagcca cagcatggac tattatatag
    30001 cagattgaaa taagatgaga acaaatcgat tgggaaagta aaattaattt ctataaatgt
    30061 tttagcaatt gtttcgtact attttagatt cagtctacta taacacattc agaaaagaga
    30121 aaaaagtctg ttgattttga ccatcataaa aagactggca atccagtctc aaacatatat
    30181 tatagaaatt ctccactaaa tactttcacg aatattcaga agcatgacaa aggcaactag
    30241 aagaaatagt aataaaacaa agctaactgc cagagttcca aagctagtag caatggttac
    30301 caaagctatt gtaaataagc taggtaaaac aaccgtaatg gcaccgatag aagattgaac
    30361 tgctcccatt gactcctcag gtatttgttt aaagacgagt tcttgcaatc taggagagag
    30421 aatacccgcg aaaaaggcat ccaaaaagct aaagatcaga atcaagccaa aatgagctgt
    30481 cgcaaagccc actaggagta gtaattggag taggagtgaa gcatacaggg ctatttttat
    30541 ggaaatggta tgttgcagat agccactcat cagacttccc aaaataagag ctgacaattc
    30601 tagtgtggct aacaaggcaa gagattgacc tgtttgtaaa ttcaaaaagg gctggttcct
    30661 taaaaataga gtggaaatag gaaccgtaac atttatcact gcttgactag tagagataat
    30721 aaacaaaacc aagagcacct tattcatatt ccatatcaat ttcgatgatt ggagcaaatg
    30781 ctggcaaaag gattttacaa agagtccttc ttgatagcta atcgtttttt ctactttcaa
    30841 gaggtcagtt tttatgaaga ggatacctaa aaatgcgatt aaaaaggtaa gagcgttcag
    30901 taaggaaata aactggatgg atagaatgcc tagtaagact cctcctagga tattactgat
    30961 tgttttcact aaactaacag ttgactgttt aaagccaata gcttctgcca gatggtcttg
    31021 cccaataatt ctaatgaaaa tcggagtgag catggcgcct gaaaaataac tcaatgtgtc
    31081 agacaagagg ttaatcagac aaataaatgc tactagcaac aaggagaaag actgccctga
    31141 aagtgataaa gacactatag agtaaagcaa aaattttgca aaactaatga ctgtgtattt
    31201 caagacacga tgatgttgaa aatccgccaa aactcccaga aagatttgta gaacttgggg
    31261 cagggtttct gaaatcgtga tgagtaaaat cgccaaaggg gcaaaagatg catctgccac
    31321 ataattcagg aaggccagat aaaaaatcgt atccccaagc gttgaaatcc actggttgat
    31381 ggttaattgt ctaaaatctc tattttgaag aaatactttc atcacaactc cttcttaaat
    31441 tcaaatggga agctttcccc aaggatagac cgcgatacta ctaacaacca aaattacagt
    31501 aacatcaaaa gctgaccaat accattgtag actatatgca gtccaatagg ccaataaatt
    31561 gactttgtca ttctaaataa gactgcaaat ataagacctc cacccatata gaagacaaag
    31621 tctgtcaaga cccaaccgtg attactaatg tgcgagaccc caaataaaac agcggaacca
    31681 agtacatcta gcccccattt ctttcctttt tccagagcag tcatcactaa tccacgataa
    31741 atcatgtctt caaaaatggg acctgcaatc acaggataaa aaaaatacat caaaaatgcc
    31801 gtagctcctg taaaagtagg agcagcatgt tgataagaaa tttcatttcg agtaggtggg
    31861 aaaagaaaaa aggtgacaaa attccaagca acaaaagcaa gcagagctag gaaggaatag
    31921 aaaagatagg atcctttaaa ctttctacta ttgattttct gccatttccc cgaccaaatc
    31981 atagcaataa gagcaaataa aaccacaaga aaattcaaca tcatatccga cagataatag
    32041 gcaaagtcag atagcccagc aacaaggtcg ctgcgtaaaa ctaaaacact gaacttctgg
    32101 tcagcaataa ctagtagaaa aactataata aagtagcggt gtgagattat ctttttcatt
    32161 ctatttcctc ctatacatat gaaataaaga cagcccctac ccaagaaaag gcaagggctt
    32221 tggatattaa taacgacggc ggtcagaatc aaagtcaggt ctatcgttaa aatttttctt
    32281 ccagaaatta gtgattgacc aaggtgtccc gaaccctccc caagcaccgg ctgggataac
    32341 agcattattc ccacattccc ctccataaac tgtctccaac tcagcttctg tcaattccat
    32401 tgtttctgca aattgtaaat ttaacatctt ttacactcct tcaattatct tcatttgtaa
    32461 accacttctg cgacctagga tttgcttcaa gtgctttaca agtacagtat aacacgaaaa
    32521 ttataacacg aaaattggct tattttagaa aatcgcatat ttgatatttt ttcttataga
    32581 aatttcagat ttgcgatttt ggtagatttg attatttccc tggtataata aagttattac
    32641 taatgaggag ggaagtaata tgaagaaaca agttttaaca ttattgacga tcgttgctga
    32701 tattattatc tttttcccat ttctaactaa tcgataagct ctttgtattg ctgcaaacgc
    32761 aattcaaaaa gggctgtcaa ttgtggattt tctaatactt gcagagattg gataaagtgt
    32821 tcaatctctt tttgattgct tcctttggtt tgaagaaaaa tctgcatttt ctttaaaaac
    32881 tgccacaata ctttttcaaa aacatcgtat ggacggagca tgcgctccag ttcagcttca
    32941 aagattggaa tataagaaaa tagcttgcgc tccataagtt ctgagatgat attaagataa
    33001 ccacttttca tataccatcg attgtgtact aactctttaa attccttgga tttttcgagt
    33061 aaggaggttg ataaaaagat cagatcttga ttgctcaaga agggcatagt attgcaaaaa
    33121 agatagagtt caaaccaggt ccaagactca atagcgtaga ggtaggtagt caaaaactcg
    33181 ctatcctcct ctgttagcgg gtagctttta tctaaggaat ggagtgcatt tttaatcaca
    33241 atggcattca aacgacgata ggtctgcgcc atctgttctt gatcgacttc ctccaatagc
    33301 tgctctaaag cagctatatc ctgatgggca aagcgatcca caacctttcg accgattcgc
    33361 atatgtggag attcttgata gttgttgagc ttgtgcccaa actcatcaaa attcatattg
    33421 atcccttgga tggctaatat gagcttatct gcagatagca tagactgtcc tagttcaaac
    33481 ttggacaact gagaagctgt tagaccctca caggccacat ctgactgctt aagttttctc
    33541 gccaagcgca attccttgta aaattctccc aattccattc tctcaatcat ctgaccacct
    33601 cctagctttt gcatattata tcattattta caatcatttg tcaaaatatt ctgatattta
    33661 agagggcaaa aaagccagcc ttaagctgac tctaaaagga ccgagccttg tacaatacag
    33721 aactcaatcc tcgttctaaa tagaatgtct aacttttttg ggcagtacac gagcttgggg
    33781 aacagccact ggaacgatga ggtgtgagct caaaatatcc tccagttatg tttttcctaa
    33841 tagtataccg gaagagtgaa aggattttat aatggagcgg ttacaaagaa cctactttct
    33901 attaaacagt atactatgaa aatgtgaaaa tttaacattt ttttgtacaa attttataaa
    33961 ttattgcctt tttaatatca atagttaatc tcttatccag atcccccttg tgtaaacttt
    34021 atctttataa gcttcaaggc ccctatccca tctatttaca acaattagat cactttgttt
    34081 tgtaaatagt tcaaaattct tttcaaaaat tacgttatct atactaacgt ttaaatttgg
    34141 ttcatatact aaaattttta taccgacaat caat
//
LOCUS       DAVKJU010000019        31801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVKJU010000019 DAVKJU010000000
VERSION     DAVKJU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.019"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..297)
                     /locus_tag="VW191_000819"
     CDS             complement(1..297)
                     /locus_tag="VW191_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623609.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VW191_000820"
     CDS             complement(281..2440)
                     /gene="pfbA"
                     /locus_tag="VW191_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEU9623610.1"
                     /translation="MLKIVKKLEILMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIIFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRENGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(3571..4797)
                     /locus_tag="VW191_000821"
     CDS             complement(3571..4797)
                     /locus_tag="VW191_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEU9623611.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKIVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWRANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4840..5532)
                     /locus_tag="VW191_000822"
     CDS             complement(4840..5532)
                     /locus_tag="VW191_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEU9623612.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLAFVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5705..7456)
                     /locus_tag="VW191_000823"
     CDS             complement(5705..7456)
                     /locus_tag="VW191_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623613.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(7446..9191)
                     /locus_tag="VW191_000824"
     CDS             complement(7446..9191)
                     /locus_tag="VW191_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623614.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYTTSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9397..9774)
                     /locus_tag="VW191_000825"
     CDS             complement(9397..9774)
                     /locus_tag="VW191_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEU9623615.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVALKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYK"
     gene            complement(9846..10302)
                     /locus_tag="VW191_000826"
                     /pseudo
     CDS             complement(9846..10302)
                     /locus_tag="VW191_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10435..11262
                     /gene="xth"
                     /locus_tag="VW191_000827"
     CDS             10435..11262
                     /gene="xth"
                     /locus_tag="VW191_000827"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU9623616.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11274..11417
                     /locus_tag="VW191_000828"
     CDS             11274..11417
                     /locus_tag="VW191_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEU9623617.1"
                     /translation="MDYNAVIPELLVSNIEQSRSFYCDLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            11528..11656
                     /locus_tag="VW191_000829"
     CDS             11528..11656
                     /locus_tag="VW191_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEU9623618.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHEFAVLDPNGYFLRFSE"
     regulatory      12039..12134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12534..13115
                     /locus_tag="VW191_000830"
     CDS             12534..13115
                     /locus_tag="VW191_000830"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEU9623619.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFATTGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIKIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13115..14377
                     /locus_tag="VW191_000831"
     CDS             13115..14377
                     /locus_tag="VW191_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEU9623620.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIAMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIFVASLLAIVLNAVLNHKKK"
     gene            complement(14464..15330)
                     /locus_tag="VW191_000832"
     CDS             complement(14464..15330)
                     /locus_tag="VW191_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HEU9623621.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLGLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15317..16087)
                     /locus_tag="VW191_000833"
     CDS             complement(15317..16087)
                     /locus_tag="VW191_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU9623622.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16113..16967)
                     /locus_tag="VW191_000834"
     CDS             complement(16113..16967)
                     /locus_tag="VW191_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEU9623623.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELIPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17124..17528)
                     /locus_tag="VW191_000835"
     CDS             complement(17124..17528)
                     /locus_tag="VW191_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU9623624.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(17598..19079)
                     /locus_tag="VW191_000836"
     CDS             complement(17598..19079)
                     /locus_tag="VW191_000836"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEU9623625.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWETYVHYPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(19098..20276)
                     /locus_tag="VW191_000837"
     CDS             complement(19098..20276)
                     /locus_tag="VW191_000837"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU9623626.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGFVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAFDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEVFKEAVGKHYEEVVGYAPSFYIAEVAGGTHVLD
                     "
     gene            20440..21447
                     /gene="galR"
                     /locus_tag="VW191_000838"
     CDS             20440..21447
                     /gene="galR"
                     /locus_tag="VW191_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEU9623627.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21493..22530)
                     /locus_tag="VW191_000839"
     CDS             complement(21493..22530)
                     /locus_tag="VW191_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9623628.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22531..22884)
                     /locus_tag="VW191_000840"
     CDS             complement(22531..22884)
                     /locus_tag="VW191_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU9623629.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23128..24018)
                     /locus_tag="VW191_000841"
     CDS             complement(23128..24018)
                     /locus_tag="VW191_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU9623630.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDV
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALSRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24151..24687
                     /locus_tag="VW191_000842"
     CDS             24151..24687
                     /locus_tag="VW191_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9623631.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24853..25659)
                     /locus_tag="VW191_000843"
     CDS             complement(24853..25659)
                     /locus_tag="VW191_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEU9623632.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLPN"
     gene            complement(26004..27524)
                     /locus_tag="VW191_000844"
     CDS             complement(26004..27524)
                     /locus_tag="VW191_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEU9623633.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(27517..28245)
                     /locus_tag="VW191_000845"
     CDS             complement(27517..28245)
                     /locus_tag="VW191_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623634.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(28260..29024)
                     /locus_tag="VW191_000846"
     CDS             complement(28260..29024)
                     /locus_tag="VW191_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU9623635.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     CIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29028..29471)
                     /locus_tag="VW191_000847"
     CDS             complement(29028..29471)
                     /locus_tag="VW191_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9623636.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30153..30449)
                     /locus_tag="VW191_000848"
     CDS             complement(30153..30449)
                     /locus_tag="VW191_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9623637.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(30471..31535)
                     /gene="pepA"
                     /locus_tag="VW191_000849"
     CDS             complement(30471..31535)
                     /gene="pepA"
                     /locus_tag="VW191_000849"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEU9623638.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         9461 a         7145 c         5648 g         9547 t
ORIGIN      
        1 ttatttcatt ttactatact ctgttaattc atatgagttt aaaccgattt catctttaac
       61 ctcgagtaaa gcagtttcaa atatttgttt aagagttttt gattctttac aattaaccga
      121 caaactttct gataaaatat gtacaacttc tgagactgaa taacctatct cctctttaga
      181 attatataaa tctgtagctc caccaataat ccaaaaatac tgattttgtg aacctacaat
      241 atcctcattt tctacggaac ttccttgtat cgaacaaatt ttatttatct ttaccataat
      301 acttcaaccc ttttagtgtc aaaagtaaac caattcctgt cactgttaag aatagttcca
      361 taatcttgtt cgaaccagtc tttggtaatt tttgttttac atctactatt tctttagatt
      421 tattaatatg attttcagtt tctctgccat ctccaactat tttatagttt acttcttctg
      481 tcttattatc ttgtttattg tcgatcttgt cattcatttg tctattatct ttacttgagt
      541 taaactctcc gttcttctgg ttactatcaa ttacattatt tgaattagat tgtttttcct
      601 ctttgttttt ttctttttcg tttttatcac ttaaattatt tgttacaatt ttgtaaagcc
      661 cattctccgt tacaatattg aaattaccat cgctatcacg tataacaggt tctttcccat
      721 ttgcattaga tttgatgaat gatatatact taccggataa attataaaat tggttattta
      781 aaacggttat tttacccttt gaatcctcaa taacaattcc ttctttacca ccgaatatac
      841 tattctctga gactactaaa ttatcactaa ctcgtaataa ttcaatattt ggttgttctg
      901 tttccttaga attattatta attacatttt ttgttacagt aatatctgat acttttccta
      961 aatattctgc actatctttt gcaactcgta tcgcttttgt tttaggatcg gcaatattaa
     1021 atatattttc ggcgataacc acctgtttat ttaaatctaa taggtcttta gtgtttttat
     1081 agctataagc atttactaaa gctgctccgt tttctcgata atgtacactc tctcctttaa
     1141 ctttcttatc aaagcgattt cctttgatta atacatcagt gaatcctgta aaacgtacac
     1201 ctgcatacat catgttatca aaatgattat ttaaaatttt aatattagag gggttctgtg
     1261 tcgacaatgt ttgatagtgt gtgccaattg ctgttactaa ttccccagat ttatcacttt
     1321 tgccaaaata ggaattttga atagtcacat tttcagattt tttcccatca tcattcaagg
     1381 cataaggaaa accttttcta gttaatggtt caatctgaat gctctcctta cttatgattt
     1441 gcccatcctt catcgttttg ggtaaggctt gcccaagaaa acgagaatta tcaactaata
     1501 catttttcga acctgcaatt tgaatagcat gcccttgata actatccttg aatgttacat
     1561 tttttatagt tacgttattt gaattcccaa tagcaaatgc acctgaagaa tttataagtg
     1621 gtagattttt tgctttagtt ccttcttcat tcaaagcacc gttcatatca atcgtaccac
     1681 cagaataact aatatcttct gttgggcccc attctacttg cgcaccatca tccgtaaata
     1741 aacctgtcat aaaaacaatg gaagggtgat tcttaatatt tataccattt agaattgtag
     1801 ctttctcatt caattctaag tgtgtatgac tttttaaaaa aataatttct tttactaaat
     1861 aagttccttc aggaaaatat acatttcctc cacctagccc ttgagctgca gcatctattg
     1921 catcttgaat tgcttgtcga tcatcatgaa tcccatcacc taccgcacca taatctttta
     1981 cacttaaagc agtatcttca aattcttttt tgataattct gtcacttata acatcttcct
     2041 tataactctt attttcatta gtagatgtta ctgtagattc aactgttctg ttgtctaatt
     2101 gtgaattgga acgaatagta ccattagagc ttatactatt aacacttaac gaattatcta
     2161 aaccattagt ggttgaatta gaggcaatca tttcagcaga acgattctga ttaacagttg
     2221 gtgaattatc taaatcatta gtaattgcat tagaagactc ttttgtagcc atcggtgaag
     2281 aactagtaac aacttcatcc gataaaatac cttggcttcc caaaattgaa attgccaata
     2341 gtaccgagca agtccccacc tttaatttac gaatactgaa tacctcatta ggaacaaaat
     2401 atttcatcag aatctccaat ttttttacta tttttaacaa ttgattatta cttttcttaa
     2461 aatagataga atgacaccgc tgtcattatc tttatcatat attttccaaa ttgaattgtc
     2521 aaggaataac acaagaaaat attaaaattt atactcttcg gaaatctctt caaactaggt
     2581 cagtgccgcc ttgccctaag tctaggttac tgacttggtc agttctatag tagactgaaa
     2641 ctaaaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac tgtcccgatc
     2701 aatttgtcct attcttattt cattttacta tatctacaac ctcaaagcag ggttttgagc
     2761 tatctatggc caacttccta gtttgctctt tagccttcca ttcaaaagtg atgctatctt
     2821 tcttagcttg aatataaaat atcccattta ctatatttaa atttattgat atgttgtaaa
     2881 ttataaatga aagaacaaat accttgtcat acaagcattc gccggggata tttacctgaa
     2941 tatcgctcat atggcggatt gaaactaaaa cactacaata tatttcttaa atagaccttc
     3001 tataaaagct agatttaacc tcgataaaaa tagatgcagt gcttctaaaa tactggaata
     3061 gggcatatag gtaatcgtag acaagtgttt tatagattgt ctcgcgggag atctaatgat
     3121 gtatagcaag catcactaaa catatgggat attcgtatta ttataatatc aacatgctat
     3181 aaattctatc atgcaaaaga atgactagaa catgaaagac aggcacaaaa ctttgaacaa
     3241 tattctgaaa acaaaaaaaa gacgcgagtt tccacaatct cactcatgga aactcacgtc
     3301 tttcaaaaaa tagggtgaat tgaaaactag aacaagacac tagattttct aaatgatgtc
     3361 tagtaaggat tctaaatgct acaccaaatt gctcacatct tatttcaaac cacgggactc
     3421 ccacaagaaa ggaaaatcct ataaaaattc tctctctgag gagaaagagg cctcaactac
     3481 aggtgtaaac tattatcaat tgaaaacaac agtttttaca ctcaattaga ctttttaggg
     3541 acaggatcac tttcgcttga ctgtagtttg tcattttttt gataaagtta gcactttttc
     3601 agaaactgtt ttgaaagtct caatgatata gtctacttct tcatcgctta atttagtatg
     3661 aagagggagg gtaatttcat tctcaaagaa ggcataggcc ttaggatagt tcgtcatatc
     3721 aaatccaaga ttcttatagg ctgtcaagag aggaagcggt ttgtagtgaa cattacttgc
     3781 aattcctgct ttagccaatt cttggatgat gaggttgcgt tcttctaggc ttgctccttc
     3841 tacacgggtg atgtagaggt ggcgtgaaga ttcgacagtt tcagtcttgt gtgccaaagg
     3901 atggatgcga gaacctgcaa aaccactatc atagcggtcc acaatgtcct tacggcgttg
     3961 caacaaactt ggatagcggt ccaattgtac caaaccaagt gaagccatga tatcggtcat
     4021 gttgcactta taggctggtg taacgatatc gtattcccat gaccccagtt gcatcttggc
     4081 aagagcatcc ttagtttgcc cgtgaaggga aaggatttgg aattccttgt acatctcttc
     4141 gtcatcaatc actggattgg ctctccaagt cgcacttcca ccttctgccg ttgtaaagtt
     4201 cttaacggca tggaatgaga aggaagtaaa gtcagcgata gaaccagaag gttgtccttt
     4261 ataagtagat cccaaagcgt gggcactatc agagacaatg acaatacggt taaaggcctt
     4321 ttgccacttg cttgaagcgg taaagaagtc acgttttttc tccacgactt ggaacaaacg
     4381 gtcataatcg caaacaatcc ctgcgagctc tactgggata atcaccttag ttttctcagt
     4441 gatagcttgc tcaagcaggt catagtccat ctcaaacgta tctgcttgga tatccaccat
     4501 gacaggggtt gctcccacgt gcgtaatgac actacatgaa gccgtatagg tcatggctgg
     4561 aacgatgact tcatcaccag gtcccacttc caaaacgcgt aaaatcaact ccagagcagc
     4621 tgtcgcagag ttgagacaaa caatcttagg tgtctgtgtg taaagagaca agcggcgctc
     4681 cagttctttt gttttaggac ctgttgtgat ccaaccagaa cgcagggtat ccgctacttc
     4741 agcaatttct gcttctgtga tatcaggcgg tgaaaatgga atattgtaat ttggcattga
     4801 tttgctcctt ttatctgtac tcattttctt atgactactt tattttagta cctcaaacac
     4861 ggtttgaaac atgattttga tgtccccaaa gaaactaaac tctcggagat aggcgaggtt
     4921 atagcgcatc ttttcaggaa gaacatgctc cacataggcc tgatcaactg acagaccttt
     4981 ctccgtcatt tgactgatga tggtgtcctc atccttgtag ttgatgctgg ctggagaggt
     5041 aattcctgct tgcaagagca aggttgccat catttcaggg ctatactgct ctgtataacg
     5101 tggcacttca ggtcgtgtac cgacaaagga catctcacct ttaaggacat tgaccaactg
     5161 aggcagttcg tccaaacgga cacgtcggat gaaatttcca accttggtaa tacggctatc
     5221 gttagcagaa gtcaccagac ttcctttttt atccgcatcc gtcaccatgg tacggaactt
     5281 ccaaatcttg aaccgacggt tgtactgggt cacacgctct tgcttgtaaa tcactggccc
     5341 cttgctatcc aacttgatcc aaatgctcaa gatgagaaag atgggagagg tcagaaccag
     5401 taagacaaag gctagaaccc agtccaagca acgcttgaaa atcagcgaac ccttcctttt
     5461 agagacaagc tggtaataag actcaacctc gcttgatttc atttccacag gcaagtcttc
     5521 ccatttcagc atactgttct cctttgtttt tattatacta tttgtcaaca tttttcatta
     5581 taccacaaaa tggaaaaggc ggtgaaagaa atctgtaatt tgaaggattt ccttctagta
     5641 ctcaatgaaa atcaaagtac agctttgaga ttgcagataa agctgacatg gtttaaagag
     5701 attttcaaag agtataggcc atggcccctg cctgcaaact atacatcttg tgataggttc
     5761 ctcccagagt caagagttcc tcatgggttc cactctcgat aatgcgtccc ttatccaaga
     5821 catagatgca gttggcgtct tgaatagtag aaaggcggtg agcgatagca atagttgttc
     5881 ggccctgtct catcttcgcc agagaagctt gaaccaagct ttctgtttca gagtcaatat
     5941 tggctgtcgc ttcatccaaa atcaggattt taggctggct ggcgactgtt ctagcaaagg
     6001 caagaagctg gcgctgccca gtagagaagc tcgaaccacg ctcggaaaca ggggagtcgt
     6061 acccctgagg aagttcttga ataaaggaat ctgcatccac aaaggctgcc gcagcctgaa
     6121 cctgctcatc actgatttct tggtacatgg cgatattgga cttaatagtt ccatgataga
     6181 ggaagggttc ctgtaagacc aaaccgatgt tttttctcag ctcttcttga ctgaaatccc
     6241 tgatatccac atcatccaag agaactctcc ctgactggaa ttcataaaag cgcatgagga
     6301 cattgataat agacgatttc cctgaacctg tatgacctac aaaggcaatg gtttcaccct
     6361 tattaacaga gaaagaaatg tcatccagaa tcggatgttt accgtcatat gagaaacaca
     6421 catgttcaaa acggatattg ccttcttgga ctttggcttg cccattttct tgaagaggtt
     6481 cataggtcct ctcgtctatc agggcaaaga cacgacctgc agaaaccata gccgtttgca
     6541 gagttgaaaa gttttgcgtc acctcaatca aggggtcaaa aaggcggttg atgtactgga
     6601 taaaggcata catggtcccg accgttatcc cgatagaaaa accacggtag ccaaagtagg
     6661 ccatcaagac tgcatagcct agaagtttca gcaaactcat ggcaggtctc aaaaagaggg
     6721 catccaaggc tacagaacgg ttggcgtaga ccaagtgttc ttggttgatt tcatcaaatt
     6781 ctgcctgcag gcgcttctct tgattaaaag cctgaataat cctgattccc tcgatattct
     6841 ctgccagctt actattgata tctgacaaga gacttctggt tttctcgatg attttcactg
     6901 acttttttcg atagagattg accaaaagga aaatcaaggg gagaaagagc aagactaatg
     6961 ctgtcaaacg aaaatccagc accaacatgg tataaagggt tgtcagaaag ataaaaactg
     7021 ctgagataaa gctggataaa atcccagaaa acatatcact aatcgtctcg gtatcgttgg
     7081 tcaaacgaga aacgatagaa cctgctggcg tcttgtcaaa gtaagacatg cccagtttct
     7141 ccatattggc aaaggcatcc cgacgaatat ccctaacaat actgtaagac acgcgcgcaa
     7201 agagaagatt gccgacatac tgaactacag tttgtaggat gtagagacca tagtagacca
     7261 gcaaaacggt cacggctagt tggttaagat tgctgagata ctggtcgata aagtgggaag
     7321 ccacaagggg aatgacactt ttaatgaccg tcgtcgctag gagaaaactg agtgccaaaa
     7381 aggtaaggag tccataaggc ttgagatagg acatcaagcg cttcaataca gtccattgtt
     7441 cttgtttatt ctgcatcttc ttctcccttc atttccagct gctgagactg gtaggtttgg
     7501 gcataccagc catccaaagc tagcaagtct tcgtgcctgc ctcgttcgat aatttgacca
     7561 ttttgcagga ctaaaatcaa atctgcatgg acaactgcac tcaggcgatg ggcagtgata
     7621 atggttgtct tgtcctttcg cgtctccttg aggttgtcga taatcgcata ctctgtcttg
     7681 gcatctacgg cggataagga atcatccaaa atcaagatat cagggtctaa aatcatagcc
     7741 cgactcatag ccaaccgttg cttttgacca ccagaaagac tgactccttt ttcaccaatc
     7801 agcgtatcaa atccttgagg catgtctaca atatcttggt aaactcgggc tagcttagta
     7861 gcttcctcga ccgctgaaag gggcaagtta ggattgccaa agcggatatt gtctaggatt
     7921 gaagtcgcaa aaagaaactg gtcctgagga acatagccca tgagactgcg aaggtctgtc
     7981 agacgatagt cccgaatatc gtgaccgttt agataaatgg cgcccttatc cacatcgtat
     8041 tcacgcaaga ggagcttgat taaggacgtt ttcccagagc ctgtctgccc aaccaagccc
     8101 agtgtttgcc cttttgccaa actaaagtga atatccgtca gtgtttcctc attttcaaaa
     8161 gcaaagctgt caatggcata ctccaaacgc ccattttcaa taccatccag aggaaactcc
     8221 gggtcttgta caggagattc ctgagacaaa agattttcaa tccgctggta agaaaccttc
     8281 cctcgctgag tagtattaaa gaggaagccg atggccataa gaggccagac cagcatatcc
     8341 aaatagctga taaaggtgac tagattccca actgtaatct gcccttcctg aaccatcaag
     8401 gagccaacca aaagcgttaa aacataggac gaaccaacaa acaagagaac catagggtca
     8461 aagagactat catatttcat ggtttgcagg ttcttttgga aggttaattc attgactgcc
     8521 tgaaaagact tcaactcgtc tgcctgataa ccgaaagact tggtcacttt gatacctgat
     8581 acggactcct gtaccttgtt attgagttca gaaaaagcag cttgggattc gccaaaggcc
     8641 ttatgagtct ttctccctag gcgactagtc gtataggcca tgaaaggtag ggggagaatg
     8701 gcaacaagag tcatctgcca tgagatgcta aagagcatgg tcaacaaagt caccagagcc
     8761 gtgatagagg catccaccgc agacatgaca ccgccacctg ctaaacgagt caaggcattg
     8821 atatcattgg ttgcgtgtgc catcagatca cccgtccgat aggtttgata aaaggctgac
     8881 gacatttttg tgaaatgctt aaacaagcga gaccgcatga tctgtcccaa gcaataagag
     8941 gtcccaagga tatacatacg ccacacatag cgcaaatagt acataccaaa ggctgcaagt
     9001 agcaagtaaa ataggctaag aaggaggtcc tgctgagtta attgccccga tgtgatggca
     9061 tcaatgactc gccccataac cataggagga atgagattga ggacggaaac caagaccagg
     9121 gccacaatcc cgactagata acggcgtttt tctaacttga aaaaccacca gagtttttga
     9181 ataatggaca taaaatccct ttctgattgc aaatggaaac ctgaggccaa tactcaatga
     9241 aaatcaaaga gcaaactagg aaactagccg caggctgctc aaagcactgc tttgaggttg
     9301 tagatagaac tgacgaagtc agtaacctac atacggcaag gcgacgttga cgcggtttga
     9361 agagattttc gaagagtaca agaccccagg tttttcttat ttataagtta cgactgtaac
     9421 agcacccttg tcatattcag caataaagat attggctaca ttgtcatgcc cttgtttact
     9481 gaggttatca agcaaccact cttcgctacg accaatcgat tccaagacat ctacttgaat
     9541 cacaccgtca gtcacaactg gatacttagg attttcatct cccatttgca caacgatgag
     9601 ttgcccattt tgctcttgca cagctcgttt gacttgcttc atctggaaaa tcccttggct
     9661 acgaagtttg agggctacat ccgatgcaga caaaccaact gaacgacagg cttctgggtc
     9721 aatattccca tttttgataa ggagagttgg cttaccatca atcaaacgtt tcacaaagtg
     9781 aacattgttg ttgagctatt tgagagtcaa gaccagtttg attaaaattt cgatataatt
     9841 aagtgtcatt tgttcacatc caccagttca attgcgtctg tcttatacaa ttcgactttc
     9901 tctaacaggt acttgtctgg ctcacttccg tttagggcac gataatagcg atctcccacc
     9961 ttgataagtg cgccatctgt gtttgtggaa gtattaacat agacttctga cttgtctact
    10021 cccaaatctt tggaaacaac ctcgatgaaa tgaagtgaag tttgaaattt attgttagaa
    10081 acttgattgt tttgataagt cgtgatgctg accaaaagca gagctaaata aggccagagc
    10141 tgaaatcatg accaactcac gaaatttggt tcctttttta tcatggtatg ccttaaaagc
    10201 aaaaaaaact gtcacagcta atagaacaat cccaaagcca accatgatac cattttgctg
    10261 accgatttgg ctgagtacat agtcataaga gtagaatttc atgtattttc actccctttc
    10321 ataaaatcat tcttaattat aaacgaaaga gagtaatttt tcaaaccata cgttggggaa
    10381 atagatcttt ttttggtata ataaaatcta taatctgaat gaaaaggtaa ctttatgaaa
    10441 cttatctcat ggaatattga ttccctaaac gctgccctaa ctagtgactc agctcgtgcc
    10501 aaattgtccc aagaagtcct acaaaccttg gtcgctgaaa atgctgatat cattgctatc
    10561 caagaaacca agctttctgc caaaggacct acaaagaaac acgtggaaat tttagaagaa
    10621 ctcttcccag gctacgaaaa cacgtggcgc tcttcccaag agcctgcccg taaaggctat
    10681 gctggaacca tgttccttta taagaaagaa cttacgccta cgatcagctt cccagaaatc
    10741 ggtgcccctt ctaccatgga cttggaaggc cgcatcatca ctctagaatt tgatgcattt
    10801 ttcgtgaccc aagtttacac accaaatgct ggcgatggtc ttaaacgctt ggaagaacgc
    10861 caagtctggg atgtcaaata tgcggagtat ttggctgaac tagacaaaga aaaaccagtc
    10921 cttgcgaccg gtgactacaa cgtagcccac aatgaaatcg accttgcaaa tcctgctagc
    10981 aaccgccgtt cacctggatt tactgacgag gaacgtgctg gatttaccaa cctattggca
    11041 actggattta ctgatacctt ccgccatgtt catggcgatg ttcctgaacg ctacacttgg
    11101 tgggcacaac gcagcaaaac ttctaaaatc aacaatacag gctggagaat cgactactgg
    11161 ctcacaagta accgtatcgc tgacaaggtc actaaatctg atatgattga ctcaggtgct
    11221 cgccaagacc atacaccgat tgtcttggaa attgatttgt aaggattttc tatatggact
    11281 acaatgcggt cattcccgag ttgcttgtat cgaatatcga acagtcccgg tccttctact
    11341 gtgatttact gggttttagg attgaatacc aacgtccaga agaaaacttt ctctttctct
    11401 tgaagagtgt caactaatgt tagaggaggg aacaaaggat caactagctg aactgaccta
    11461 tccatttggt cgtggagtca atctctcctt tggcataaag gatgtttcaa aactctatta
    11521 aagagtgatg aaggcaaact atcctattta tcgtcccttg accaagagaa agtttcgtgt
    11581 cagtgatccc tatatctatc ctcatgaatt tgcagttttg gatccaaatg gctatttttt
    11641 aagatttagc gagtagaata ataaaaattg ggaaaagtct ttaaagttag aaaaaggcat
    11701 attgtcaggt gtctaaaaac ttgatagtat gccttttatg atggaaaaat cttctaacac
    11761 tcttcgaaaa tctcttcaag ccacatcaac gtcgccttac cgtaggtata tgatactgac
    11821 tctgtcaatt ctatccacaa cctcaaaaca gtgatttgag gttgtggata gcttcctagt
    11881 ttgctctttg attttcattt agtataaatg ttagcttaca acggagtttt tgttcacccc
    11941 ctcttctgtt tgttcgtttt ttaaaccaaa gcactaacaa aatagttgat ttataaagtt
    12001 atttagtgta aaataaaaac attggctcaa gcctatttaa aattgaatat cgttttactt
    12061 gtttatgtcg tgaattggca cgacgtttct acaaggtgcc ggaacaccta acaataagta
    12121 agtcagcagt gagatatggc cgtttttgcc atgcctattt gggggactta cttagagatt
    12181 aagtaggtct tttttgaata tttttaatat ttcccttaat tagtgcggac gggaggtgaa
    12241 attatcctgt gaataatttc aggaatccag ggaatttcat ccctaatttt tgagccccta
    12301 gctctttcat ttctaggctt aggctaaaat agttccccga actattttac tctcaaaaat
    12361 cccgtcatag acaataacaa gattattgtc tatgatacca ctacaacggg aaatatttga
    12421 taagactcgc ttcgctcgta atagttttat caaatatata gtattagcaa aagggatcac
    12481 tatagaaaca tttaaacaac aagtaaatta acttttagac tttaggaggt cttatgaaat
    12541 tattagaaga gcgcatcctc aaggatgggc atatcttggg tgataacatc ctcaaggtag
    12601 attccttttt aacccaccaa gttgacttta gcttgatgcg agagattggt aaggtttttg
    12661 cggaaaaatt tgctactact ggcattacca aggtcgtaac cattgaagcg tcgggtattg
    12721 ccccagccgt ttttacagct gaagccttaa acgttcccat gattttcgcc aaaaaagcta
    12781 agaacatcac catgaacgaa ggcatcttaa ctgctcaagt ctactccttt accaagcagg
    12841 tgaccagcac cgtttctatc gctggaaaat tcctctcacc agaggacaag gttttgatta
    12901 tcgacgattt ccttgctaat ggccaagctg ctaaaggctt gattaaaatc atcgaacagg
    12961 ccggtgccac agtccaagct atcggtatcg tgattgagaa atccttccaa gatggtcgtg
    13021 atttgcttga aaaagcaggc taccctgtcc tatcacttgc tcgtttggat cgttttgaaa
    13081 atggtcaggt cgtatttaag gaggcagatc tctaatgcaa actcaagaaa aacactcgca
    13141 agcagccgtt cttggcttgc agcacttact agccatgtac tcaggatcta tcctggttcc
    13201 catcatgatt gcgacagccc ttggctattc agctgagcag ttgacctacc tgatttctac
    13261 agatatcttc atgtgtgggg tggcaacctt cctccaactc caactcaaca aatactttgg
    13321 gattggactc ccagtcgttc ttggagttgc attccagtcg gtcgctccct tgattatgat
    13381 tgggcaaagc catggtagtg gcgctatgtt tggtgccctt atcgcatctg ggatttacgt
    13441 ggttcttgtt tcaggcatct tctcaaaagt agccaatctc ttcccatcta tcgtaacagg
    13501 atctgttatt accacgattg gtttaacctt gatccctgtc gctattggaa atatgggaaa
    13561 taacgttcca gagccaactg gtcaaagtct cttgcttgca gctattactg ttctgattat
    13621 cctcttgatc aacatcttta ccaaaggatt tatcaagtct atctctattt tgattggtct
    13681 ggttgttgga actgccattg ctgctactat gggcttggtg gacttctctc ctgttgcggt
    13741 agctccactt gtccatgtcc caactccact ctactttggg atgccaacct ttgaaatctc
    13801 atctattgcc atgatgtgta tcatcgcaac ggtgtctatg gttgagtcaa ctggtgttta
    13861 tctagccttg tctgatatca caaaagatcc aatcgacagc acgcgccttc gcaacggtta
    13921 ccgcgcagaa ggtttggcgg tacttctcgg agggatcttt aacaccttcc cttacaccgg
    13981 attttcacaa aacgttggtt tggttaaatt gtcaggcatc aaaaaacgcc tgccaatcta
    14041 ctacgcagct ggtttcctgg ttctccttgg actgcttcct aagtttggtg cccttgccca
    14101 aatcattcca agctccgtcc tcggcggtgc catgctggtg atgtttggtt ttgtatctat
    14161 tcaagggatg caaatcctcg cccgagttga ctttgctaac aatgaacaca acttccttat
    14221 cgcagctgtt tcaatcgctg caggtgtcgg tctcaacaac agtaatctct ttgtcagcat
    14281 gccgacagcc ttccaaatgt tcttctcaaa cggaatcttc gtagccagcc tactcgccat
    14341 tgtcctcaat gccgtattaa atcataaaaa gaaataaaaa aaagaggtgc gagcctcttt
    14401 tttgtttatc acaaattgat tataaataca atcaatccca attcccatct aattgaattg
    14461 gacctatttc aaattcttta aaatattatg ttccaaatcg ttaatattat ataagaatgg
    14521 taaaatatca aatgtttctc gtaaattatt cttagcctta taccaacctt gtccatcagt
    14581 aatccacata aactcaacat taggaatagc tttaatttct tcagcaatca ttttgtaaga
    14641 tctagccgtc tcatttaatt ttgaaccact tcctgaataa aaattaacct caatcagata
    14701 aagcacttgt tcatttttta tgacaaaatc aaaacgtttc acagtattcc catcattcgt
    14761 aatagcagat aaatcgacag aaaaaatttc ctctatttca ttttgttcaa tctctttaaa
    14821 taaattctct cctaaaatat acccttcagc ttcaagataa ctttgaacaa tattttccat
    14881 cgcatctcca gtccgatttt tgcgcccatt agtatccata ccaacctcaa cacctgtaac
    14941 gtagtcaact aaatttgata ctaaatgatt ttgcaataaa tcaaatatac ctgatttttc
    15001 caaaaacata gtatattctt caatactata gtttcgttta ctaaaatcaa aatagaaatc
    15061 ttttatcgga tccttaacaa taatcgtatc tctcaaacgt ttagcaataa gtagaggcac
    15121 aactttcaaa atttctggat aattttgata taagcctaaa aaatcctctt gtatattttt
    15181 actaccaata agactattca taatatttag agcaactttt atactagata catttttata
    15241 aaccttagga aaatctgtat aataagtcca gtcagctact gtgtcggtca tcgttgacaa
    15301 ccactcatca aaatttctag tttgtttcat tttctgcctc caacctcttc cttgcaattt
    15361 ttaaatattc tttttcagca tcgataccta taaatcttct acctaaccgc ttcgcaacaa
    15421 cacccgtagt gccactacca acaaatgggt ctagaatata gtcaccctct ttagtagagg
    15481 ctaaaataat gcgttctaac aaatactctg gtttttgagt tggatgtttc ccagcccatt
    15541 tttcaacttt ctttgttaaa gaaccggtcc agacatcttt catttgtttt ccatcattca
    15601 attctttcat taaatcataa ttgtagtaat gacgagcttt tttatcattt tttctggccc
    15661 ataaaatggt ttcagtagaa tgggtaaaat aacgacaaga taaattgggg gcagggtttg
    15721 ttttctgcca agtaatatta ttcagaattt taaaaccttc ttgttctaat gccattccaa
    15781 ctgagtatat gttgtgcaaa ctacctgaaa tccataccgt cccattaggc ttcagaactt
    15841 cttttgctag gcgaatccat ttacgattaa actcatgttt ttcttcgaat gaagaaattt
    15901 tatcccaatc tcctttatca acagaaacaa cttgtccccc agaattagat attccaccat
    15961 tacttaaaaa ataaggtgga tcagcaaaaa tcatatccat actttctggt ttcatttttg
    16021 ataagaactt gaacgtatct gaatgaacaa gaatcatctt atttttattg tagtatggtt
    16081 ttgtcataag aacacctcca tacttatatt cgttattttt cataatttgt gactataatt
    16141 tcagaaattt ttcctcgact tgaagatttt gctccattag ttcgggtagc ttcaacataa
    16201 tgtatattaa aatccttata caactcctct actaaagcac tagaagaatt tgataacata
    16261 acataagctc ctgtatcact caatctctta aaggcatctc ttaatcttac ttgatctgca
    16321 aaagagaatc cctcatgagt ataagacgta aatgcactcg tctcagacaa tggaatatat
    16381 ggagggtcaa aatacacaaa atctcctgtt cgaacatcta caattgcctt ttcaaaatct
    16441 cccactttaa tttctagttg attgttattt agataaactg aaatagcaga tatcaattcc
    16501 tcatcaacaa ttttaggatt cttataacgt ccatatggaa cattaaattg gttcttagaa
    16561 ttcacacgat atagaccatt aaagttcact ctcaacatat atagaatacg tgcagctctt
    16621 tgtacttcgg acatcatatc tattctttca tcacgatctg cagaacgtaa atctaaataa
    16681 tattcttttg aattatattc ctgatgaact ttcaaaattt caatcaattc ttgaggattg
    16741 tccttaattt gttgatagca atttattagt tcagcgttaa aatcattaat aactgcatct
    16801 ttaggagcca aatcaaaaaa taaagctcca cctccaacaa aaggttcgaa atacctgtta
    16861 taggttttag gtattaattc tctaataaca ggcaataatt gtcttttacc acctgtccat
    16921 ttcgtgaacg gttgtaaagt aactttcttt atttctttta tcttcatgct tttaattata
    16981 ccatagaaat taaaacacaa ctcttgtatt ttctaccaca ttactaaatt ttctatttta
    17041 gaattaaatc atatcaatat caaaaaatga gtaaggcaaa tcttataaat tccaaaattc
    17101 acctcactct attaaattac tctttatata cttacccgtc tttcctcaga cctttggcct
    17161 gtgacaaaca tggtaaaggt tgctttacag acatttctgc cctcttgatt ggtgatatcc
    17221 acatccatca cacaggttgt acgaccttga tggacacatt ctcctttaat ggtcaataca
    17281 tcgtcgagtt ttcctgcctt aaggtagttg atagaggatt ggagtgtcac cccatcaagt
    17341 cccagcgaga taaccaccaa accactgatt tggtcacata gcgtgaatag atagccacca
    17401 tgggcattgc catagtagtt gagcgatgag ttcactactt tggtcgtcac cacaacgtga
    17461 ccatctctca ttttttcaat ttcgtaattt tcaaaggcag atatagcgtc aaaatgaaag
    17521 tctttcatcg tttcctcctg atatttcaaa cgacactatg atactacttt ttataggact
    17581 gtgcaaggag aaagctccta gtctagtaaa attccgacct gttccacaaa gcgcataaag
    17641 gctgtctgcc cttgttctgt ctgcttgtag actcctgcat cctcaagtac acgcacaaag
    17701 atagcaccca cagagtcctt gacgattgca agggcttttt ctttatccgt taggtctgga
    17761 tgttgggatt tgagttggtc tgcccactcc tgatgataat cggcaactgt aacagcttct
    17821 cctacaagat agctagcgac ttgctctact tcttctttca gacgtggtgg caagattgcc
    17881 aagcccatga cctcaatcaa gccgatattt tccttcttga tatgttggac atccttgtgg
    17941 ggatgataga tgccatcagg atgctctgct gaagtctgat tgtctcgcaa gaccaagtcc
    18001 aactcaaact gtccatcacg cttacgcgct attggtgtga tggtgtgatg cggtgtcccg
    18061 tctgtctctg ctaaaatctg cacactagaa tctgaatact gacgccattc ctggaaaatc
    18121 ttatcagcta agttaatcaa atcctctttg gaatccgaag tcaagcgtag gacagacatt
    18181 ggccacttga taattccagc cttgacctgc tcaaaaccag caaatcggaa ggtcttttgc
    18241 aagggagcca attccatagg aaatacgtga cggcctccct gataatgatc gtgagttaga
    18301 atagagcccc ccacaatcgg caggtcggca ttagatccag caaaatatcc tggaaactgc
    18361 tctacgatag ccaacagacg ttcaaaactc tgacgactaa tggccatggg acgatgctgg
    18421 ccatctaaaa agatacaatg ctcattaaag taagcatagg gcgaatactg gaacccccat
    18481 tcctgaccaa ccatttcaaa acggataatc cggtggttgc tgcgagctgg gtggttgacc
    18541 cgaccatggt aaccctcatt ctctaaacaa agttgacact gaggataatt actagcttgc
    18601 accaacttgg ctgccacaat ctctttcgga tccttttcag gcttagagag attgatggta
    18661 atttcaagtt ctccataatc agatggaaca cgataagcga tatttttagc aatggccttg
    18721 agtttgatgt agtcattttt ctgactgagt tgataaaaat cctctatcgc ttgctcagga
    18781 tagtggacat aggtttccca gaagtcacga ttgacctgac tcggacaagg agtcaccaag
    18841 tccatcagtt cagtaccaag gatttcacgc gcagtctgac tatcctcaat cgtctctaat
    18901 cgaacggctt cctcaactag ctggtccttg aggtcaatca atttatccag attggtctca
    18961 acttccaaaa caccttctcc cactcgtgcc aaaacacgat tggtcaggta gattcgatcc
    19021 atttcctcaa atgaactttc agaaatgaca tgtgttacaa atttatctac taaggtcacc
    19081 atagagcctc cttttgacta gtcaaggacg tgagtgccac ctgcaacttc agcgatatag
    19141 aagcttggag cgtatccaac tacttcctcg tagtgtttgc ctacagcttc cttaaagacc
    19201 tcaacagtat ctttttgaac caaggcaatg gcacagccac caaaaccagc ccctgtcata
    19261 cgagcaccga gaactccttc ttgtgcccaa gctgtgtgaa caagggtatc taattccaaa
    19321 ccagttactt cataatcatg ctccagagaa acgtgtgacg cattcatcaa acgaccaaat
    19381 gtttccaaat cacctgcttg aagggctgct tgagctttaa gggtacgttg gttttcaagc
    19441 acagcatggc gagcacgttt caaacgattt tcatctttaa tcagatagct atattggtca
    19501 aaggcccact cgtccaattc acccaaggtc tgaatatcca aggcaacttg caactcttcc
    19561 actgcttttt cacactcagc acgacgttca ttgtatttag agtccgccaa ttcacggcgt
    19621 ttgttggtgt tcatgataac aacgacattg tccttcaaat caagtggcac caaatcatac
    19681 tctaaggtgt tggtatcaag gtaaatagca cgttgatcag cccccatacc aatagcaaac
    19741 tggtccatga taccagagtt gactccgata aagttgtttt ctgtttgttt tccgatttta
    19801 accaaatcga gacgctctaa ttttaaatca aagagatgct ctgccacgaa tcctgtcaag
    19861 agctccaagg atgctgaaga agacaaacca gcaccatttg gtatattccc ataaacataa
    19921 aaatcaaaac ctttgtcaat cacgtgccca gcttcttgca agaaatgaag gacacctttt
    19981 ggataattgg tccagttgtg ctctttttca aacttgaggt cagcgagagg cacttcgata
    20041 atgcccttgt cctcaaagtt agctgagtag aaacgcaaaa cttggtcgtc acgcttacga
    20101 gctgcaccgt aagttcccaa ggaaatagca gcaggaaaaa cgtgcccacc gttgtagtct
    20161 gtgtgttcac caatcaaatt gatacgacct ggtgaaaaga aagtttggtc tgcttcttga
    20221 ccaaaaacag caagaaagtc tttgcgaaga gtttcagcag taagatgttg tgtcatatga
    20281 tttctccttt gactgtccgt taagccacct taacgtgtaa tcgttttctt ctattgtatc
    20341 caagggattt cccaaggtca atccttttta ctaaaatttt actaaaatat cagtaaaaag
    20401 cagaaaaata tgatatacta acctaatcaa ggaggattta tggctacctt aaaagacatt
    20461 gcacagctag cctctgtctc tatcgcgacc gtatcccgtg tcctcaaccg cgaccagagc
    20521 ctatctgtta cagaagaaac cagacaccgt attttaaccg ttgctgaaga gctgggctac
    20581 accaagcacc tcaagacagg cgagtcccat aaacccaagc aaaagattgc cattatccaa
    20641 tgggtcagcg aacaagggga gctggacgac ctctactact accagattcg cctaggaata
    20701 gaaaaaagag cccaagagtt ggactatgat atcttgcgct attttaatga ccatcctttt
    20761 accttaagcg aggaagtgat tgggattctc tgcattggaa aatttagccg agctcagatt
    20821 tctgcctttg aagaatacca aaaacccctg gtctttctag acagtgatac actttccctg
    20881 ggacatacct gtattatcac ggatttttac actgctatga aacaggttgt cgattatttc
    20941 ctcagccaag gaatggaccg tatcgggatt ctaacaggcc ttgaagaaac gactgaccaa
    21001 gaagaaatca ttcaggataa gcggctggaa aatttcagaa actacagtca agcaaaagga
    21061 atctatcatg atgaactggt ctttcaagga aactttactg cccagtctgg ctatgacttg
    21121 atgaaggaag ccattcagag cttgggagac caactcccac cagcattttt cgcagccagc
    21181 gatagtttag ccatcggtgc ccttcgtgcg ctccaagaag ctggaatcag cctaccagac
    21241 cgtgtcagcc tcatttcctt taacgacacc agcctgacca agcaggttta tcctcccctc
    21301 tccagcatca cagtttatac tgaagaaatg gggcgagcag gtatggatat tcttaacaag
    21361 gaagtcctcc acggtcgcaa aattcctagc ctgaccatgc tgggaaccag actgacatta
    21421 agagaaagta ccctaaatca agaataggat aacataaaaa acgaatagag ttctaaaact
    21481 cctattcgtt ttttattcga ttacaatcat agacttaatt gtcttacgtt catccatatc
    21541 tttataggct tggtcgatat cttccagttt ataacttgaa gtaaagacgc gacctggatt
    21601 gatatcacca tcaaggacgg cttttagtaa aaattgctta tcgtatgttg tagcagaagc
    21661 tgccccacct gctacagaga tattttgcat aaatgtcgaa ccaagagcac gattattata
    21721 gtgtgggact cctacaaagc ccatacgccc tccattatga agaacaccta gcgcctgttc
    21781 tatagcagcc tccgtaccaa cacattcaag tgctgcatct gctcctccac cgaggatttc
    21841 acgcaccttg gtaattcctt cttgaccacg ttctgcaaca acagctgtcg cacctgactc
    21901 catagccatc ttttgacggt cttcatgacg gctcataagg ataatttgtg atgctccacg
    21961 catcttagcc gcgatgacag cacattgacc aacagcccca tcaccgataa caacaacctt
    22021 gtcccctttt tgaacatttg caacacgcgc cgcatgatag cctgtcggca tgacatctgc
    22081 aagagtcaaa agggacttga gcatcccttc tgtatagtca gaaggttgac cagggatttt
    22141 aaccagcgcc cagtttgcat agtggaagcg aatatattct gcctgaaaat caccccccaa
    22201 attattgcca atatgattgt cgcaagaacc gtcaaatcca gcaagacagg catcacactc
    22261 accacatcca tgtgtaaaag ggacaatcac aaaatcacct ggtttcaccg tcgtaatggc
    22321 ttccccagct tcttcaacaa tcccaatcgc ttcgtgtcca ctatttttgt gtccagcttt
    22381 cgtttctgga ttacggtacc tccataaatc tgaaccacaa acgcacgcac gaaccacacg
    22441 aataatcaca tcatccgctt ctattatctg cggacgttca atgctagcaa gtccaacctg
    22501 acctgccttt gtatatactg ctgatttcat ttaaaatttt ccttccttat aaagtttaat
    22561 tttgagattt aaacgattta aagctgtctg tagctgagac aagcgctcct ctaatttttg
    22621 cttttcctct tctaaaatac caagcctctc ctctctcgtt tcatctccct tttggtagag
    22681 cgacatatag tcaactaaac tatctacaga gacacccgcc gaacgaaaac acttaataaa
    22741 ttccagcgct tcgatatcct gatcttgaaa atcacgaatc ccagtagcag tacgagtaat
    22801 cggtggcaca agaccaaccc gttcataata ccgaatcgta tccgctgaaa ttcccaacaa
    22861 atcactggca gatttaatat tcacacgcta acctcctctc atttcattca ctgaattcac
    22921 gctattctaa ctcacttatc ttattatata actttgagtt gactccaagt caagcatttt
    22981 gtatctaatc gaaaaatttt tatcattaac aatcatatat ctggagttta tacaatgctc
    23041 ttctcttacc aatttttgaa atatttaaat aataaagaaa ttactctatt ccaaataaga
    23101 tagtacccaa gtatttttca cttttttcta atgttgatgc tcataactcc gttccaagtc
    23161 acacaccttt cgcttatggg tttggtgagt ttctaggtca gcatcaattt caatggtaat
    23221 attttgaaaa ccacaatctt ttaggaaaat tcgaatagac tctttacaag tttccatatg
    23281 ttccatttct tttagacaaa catggacaat ggcatttttt tccaaagcat ccatagtcca
    23341 gagattaagc tgattaaggc tggccacatt gtccaatcgt tccaggccac tctttacttg
    23401 cttgatatca agaccttctg gcacagcatc caagaaaatc ttgagtgtag accaaaaacg
    23461 tgaaagggct tttgaaagaa taaagaaaga aatgacaagg gacaaaagag gatctaggat
    23521 ataccagtcc gtaaatcgaa gaacaatcgc catcaggata acagctaccc accctagcgt
    23581 atcttccaga aaatgcagac tcagaataga ctcattcttt gtctttccct taccaaccac
    23641 cagactcgct aacagattga tagtaatcgc aataattcct aaccagagaa tcccctcatc
    23701 attgactggt tgcggatgca aaatcttcgt gacattttcc aaaatgacta gaacagagcc
    23761 tgttacgaga atcacagctg ttaccaaggc tcctagcagg ctaaaccgct tatagcccaa
    23821 ggtgtactga ttgtcttctt cacgattgga gattgtttct agaaaagctg atattccaat
    23881 tgcaatcaca tctcccaagt catgcacaga gtcagcaaga acagcgctag aaccaaatac
    23941 tccacctgca ataaactcaa caatggcata agtcaaattt aagaaaaaag ccacccaaac
    24001 agcatatttt gccttcatat ttctcattcc tttgttataa taggtttatg aacaccttgt
    24061 tcattatcat tatccactaa aactcaaaga aaggatagaa gcaaaacgtc agctttattc
    24121 agttctgaac aatttgcctt aagtgtccat atgactaaca ttgaccgccg tatcagcaaa
    24181 accaaaaaag ccatctatca agcttttata caacttttga atgctaaggg ctacgaggcc
    24241 actactgttc aggatatcat tgatctcgca gatgtgggaa gatccacctt ttactgtcac
    24301 tatgaaagta aggagcttct tctggaccag ctctgccgct acctcttcca tcatcttttt
    24361 gaaagagagc aagccatttc aaccgaggac tacctcgccc acctctttct ccattttcat
    24421 aaaaaccaag accatatcac cagtctgcta ttttccaaaa atgactactt cctccgtcaa
    24481 ctccataaag agctagaaca ccatgtctat tccgtgctag ctgataattt aaaagaagcc
    24541 caccctaata tgcctacttc ttacctccaa cacttggtcg tgtccaactt tatcgagaca
    24601 ttgacctggt ggctcaaaaa aggtcaagat ttcacagacc aggaagttgt ccaattttat
    24661 ctagaccttc tcattcctaa aaattgaata tagagtaaag ctcagttgtc ttatttctag
    24721 gttactgagt tttttatctt ttcaacaaca aaagaggacc cgccgatcct ctttttcata
    24781 ctataaatcc ttgattatca actatatctg ttttaatcga aatctcaaaa cagcactttc
    24841 aaacatcttt tcctagttag gtaaatcagt attctgctta gctgccttgc tccattgata
    24901 ccaaccaact agactgttaa tgagataaat tagatatttc ccttgaattt gcaggctttc
    24961 tccccaccag agatagattg aaaaaacatt ggtagccgcc cagaatatcc actgttcacg
    25021 gtaaacagct gtcatgagga tttgccctac cccattggtt gcatctgtga ttgaatcacg
    25081 atagggacga ttggcaccaa tagactgata aatgaagcca aaggccaacc accaaagcac
    25141 actaatggaa agatactttg tccagccctt gccgtccagt ttacgcgcga caaactcctg
    25201 cttttccttc ttaaactgtg cctgataaat ccaaactaga agtccaattg gctgcatgac
    25261 tgtgaagtaa agtgtcgtca gcacctcacc ataaaagcct ttctgtaggg ccaaaataag
    25321 gtaaataaca gagttaatca agccaaaaag ataattactt gctcgacctt ccgatacaaa
    25381 gattacacag ataatccctg tcaagctaca aatcatccca atccagtcaa caatacgatg
    25441 ttcgtaaacc aactccagcc agagaggaaa acttcctaaa accagcaaat aaatccactg
    25501 ggcaaaacta cgatgggcaa agaggtcatc ccagatagcc ttcatagttc ctgaaaatcc
    25561 taaatcagcc atagccgcaa ccatacgacg gtaaccacct gacatttcac ctagggttgt
    25621 tttgatattt tcaattttct tttgcaaata agtatgcatc atttctcctt ttgtttttaa
    25681 agagccgtgt ctggatagac tttcggacgc aacgctctat tagataatga actgcctata
    25741 cacaagattt ctaaccttag tcgacatgag ctgaaacctc ttatttgtta agtagttcac
    25801 aaaatattat acacctattt tatgaatagt caactgtctt tacagtaaaa ttttagaaaa
    25861 tcatgaaaat tttctctttc tttccatttt aagtgacatt cagtcattct cacatcaaaa
    25921 aagcccagac gaaactgtct gagcattctt ttatctagtc gtttaaggaa gttgagttca
    25981 gtatgtttaa agtctctgtc ccatcatttc ttcaacaaac cttgttcttg gagaaactcc
    26041 ttggctactt gctttgctga cttgccttca acaccgactt ggtagttgag ctggctcatc
    26101 tggctttctg taatcttacc agccaatgta ttaagaactc tttccaactc tgggtgtttc
    26161 ttgagaagag cttctttcat gagtggagcc ccttgataag gtgggaagag ttgcttgtca
    26221 tcttccaaga cctgtaaatc ataacgctcc aattccgcat cagtcgaata ggcatccgtg
    26281 atttgaatat cccctgactg aattgcctga taacgaagag ctggctgcat agtcgccaca
    26341 ttgagattga gaccatacat tgattgcaag cccttatttc catcttcacg gtcgttaaac
    26401 tcaagcgtaa agcctgcctt cagctgccct tccacttttt tcaaatccga aatggtcttc
    26461 aagccatatt cttgagcaat ctttttcgga acagctacag cataggtatt ttgataagac
    26521 ataggtttga gataggctag atgatcctgc ttggcaatac catcacgcgc cacttgataa
    26581 acctgctctg gctcatgact caccttgggt gatggttgaa gcaaactttc agtcaccgta
    26641 ccggtaaatt caggataaat gtcaatatca ccttttttca gagcttcata aaggaaactt
    26701 gtcgtcccaa aattcggttt aacagtcgca gtcatgctgg tattttcttc aatcagcaat
    26761 ttatacatat tagccaaaat ttctggttct gggcctattt tcccagcaat aaccaagttt
    26821 tccttctctt tttgagccaa aagggctgga ctataagaca gacccagtaa taaagccacc
    26881 aaggcaaaac cagagaaaat cgtccgcaat ttcgcttttt ccattacttt tagtaggaag
    26941 ttaaaggcaa tagctagcac tgcagaagaa agggccccaa tcaaaatcaa actggcatta
    27001 ttacggtcaa ttcccaacag gataaaggaa cccagtcccc ctgccccaat caaggtcgcc
    27061 aaggtagccg taccgataat caagaccgct gccgtccgaa ttccagacat gataacaggc
    27121 atggcaagtg gaatttcaaa cttcttgaga cgctcccatc tggtcatccc aaaggcaatc
    27181 ccagcctctt gaagactcgg atcaattccc ttcagcccag taatagtatt ttgtaaaatc
    27241 ggaaaaatcg cataaatcac tagagctgtc aatgccggta aagtcccaat tcccatcaag
    27301 ggaataaaga gtcccaacaa ggccagagac gggatggtct ggaaaatacc tgcaatctgc
    27361 aagacccagt cggccagctt ctcatgatag cgaagaaaaa cagccaaggg aatcgcaagc
    27421 aaaatagcta gtaacaaggt caaaagtgac aactgcaaat gttgagatag agctgtcaac
    27481 caatcactaa aacgatcctg aaaagttgca attaaattag tcatgaacac tacctccaaa
    27541 caagtctgct acaaagtctg ttgcaggcgc ttttaaaatt gtctcgggat tcgctacctg
    27601 gcgaatttct ccatcctgca agacagcaat acggtccgcc aacttcaagg cttcatccgt
    27661 atcatgggtt acaaaaatcg ttgtcatccc aaactcttta tgcaattctt ttgtcagaac
    27721 ctgcaactgt tttctcgaaa tagcatccaa ggccgaaaag ggttcatcca tgaggaaaat
    27781 cttgggctga ccaatcatag ctcggacaat accgacccgt tgctgttctc caccagataa
    27841 ttcactaggt aagcgatgcc catactcggc tactggtaaa ccaaccttag ccaaaagctc
    27901 ttctgttttc ttcgtaattt cttccttgct ccaccccttc atttcaggaa tgagagcaat
    27961 attttccgca actgttagat ttggaaaaag agcaatagcc tgtaaaacat aaccagtaga
    28021 aagacgaagt tcacgctcat catagtcttt gatgcgcttc ccatccatat aaatatttcc
    28081 atcagttggt tccaaaagac ggttaatcat cttgagcatg gtcgtcttac ctgacccaga
    28141 aggccctact aaaaccataa attccccatc ctcaatctgt aagttgacat ctctcaagac
    28201 atccttttct gtgtagcgca gtgctacatt tttgtattca atcattcttt gtcctcaatt
    28261 taaaacttcc ctcgattagt caagtcttct accttaggca tgacttcctt attatcccaa
    28321 tgctccacaa ttttcccgtt ctctaaacgg aagatatcgt actgggcata agcaacgcca
    28381 tcaatctgag tctgaccata gctaaccaca tagtttcctt gtcctaagag ttggaaaaca
    28441 aagtcaaaag tgacactata ttcagccaca tagtttttat aagcagcact tccttgtcca
    28501 atctcatgat tatgctgaat caaatcgtct gccacataat cactccactg ctctagctcc
    28561 ccattttgga aaatttctgt caagaaacgg cgaaccagct ttttattttc tgctttctta
    28621 tccaaatcct tgatttcaaa atctccaaaa atttgatcta gttggtcatt ttcaggtgtt
    28681 cgatagtagt caatgacatc ccaatgctca acaatacaac cattctcatc cgcacggaaa
    28741 gtatccgtcg tcacccattg agcttctcca ccattcagat attgatgaac atgaacaaag
    28801 accagattgc catcctcaat ggtgcggaca atcttaatct gacgctctgg atgacgctca
    28861 aagaaatctg caaagaaggc tgcaaatcct tctttcccgt caggaacacc tgtcgaatgt
    28921 tggatatagg tatcccctac agactgggct tgagcctcag caactcgtcc gtcttgaatg
    28981 gcatggatgt ataggttgtg agcatttttc acttgttgtg acatattcta aacctcattt
    29041 cccttctctt tcagattcgc caaaattctt tcttgaaaac cttcaaattg gtgaatttct
    29101 tcctctgaaa atcctttgta aaagatagta tccaatttct gactgacacg atgccccact
    29161 tctttctggg acttgcctaa ctccgttaaa actaaatact tcttacgctt gtcttttcca
    29221 cacggactaa caattacaag cttttgttcc tctagctttt ttatcatagt cgtcagcgta
    29281 ttattcgcaa gcccagtcgc aagcgcgata tctgtcgcag ttgcgcagcc agtttcacta
    29341 ttccataaaa ccgctaaaat cttgccctgt tcacccctat aaagagcctc aggatcttga
    29401 ctcagtaact tttgaaaaat ccgcccattc aacaaacgaa tatgatgggc tagcaaatga
    29461 ctatctttca taacacctcc aatttatttc gatatcgaaa tgaataaaac aattgtaaca
    29521 ctcatcgttc taactgtcaa ctatttcgat ttagaaataa tttttgataa ttatccacac
    29581 caccatactc cggctcaact aacttttaac gagagtttct aaactccttc gtcctccagt
    29641 ctacaaaagc cttccatccg tactatccta ttttttatga ggggacacat ttttcctatc
    29701 agaccattta ttttaaagat agaagtaaat cataattgct tccatctgtt cttttatagt
    29761 atattgaagt tggactaggg cactgtatct tctaaaacat tgatagaaag cgatttgaat
    29821 ttcccaatca atttgttcgt atttatagca ttttgcaact ggaataggac accatgactg
    29881 ctaaaagatt tctataaatt catttaattt cctcaatcaa tttgttcata tcttatttca
    29941 tcccgttata atttcgcctt accctatctt tttcgtagca cccttcaaac agcctatccc
    30001 ctaccgtttg acgattcctc acttcactcc acttccatta cagaagtttc ttcactacta
    30061 tggtctcggc taacttctca tgattccttg ttactactat ttgaacgctc acgagatagg
    30121 tcttataaaa aatgctttga tccacaatgg aatcaaagca ttttaaaaag ttcctcatac
    30181 ataagcgcag aagtcgcagt tcctctgtac ttggcttctt ctcttttgac aaagcgagcc
    30241 aagttgagca actcaggtgc tggatgtttg ggatttagga gcaattcacg attgaccagg
    30301 cttgagagac gaactgcctg caattgctca tttgtagtag gcagtttttt agtagtctct
    30361 aggagagcag caactaaatc ttcactcaaa tcatgtcgag catgattgta aagatctttt
    30421 ataaggcttt ctaggtttgg ttctgccatc cctaccacct cccttatggt ttaataatgt
    30481 ttaatcaaat caaccgttga acgatccaat ttcttcacca aggcttgtaa gaaagcttgc
    30541 gcttctagga agtcatccat tgcatagagg gtttggtgag aatggatata acgagcgcag
    30601 acaccgatag ttgttgatgg gacaccacca tttttcagat gagctgcacc tgcatctgtt
    30661 ccgcctttac cacagtagta ttggtacttg ataccagctt cttcagccgt tgtcaaaagg
    30721 aaatccttca tccctgggag aagcaagtga cctggatcat agaaacgaat caaggttcca
    30781 tctccaattt tgccttgacc accgtagaca tcacctgctg gtgagcaatc aactgcgagg
    30841 aagacttctg ggtcaaactt ggttgtagag gtatgagcgc cacgcagacc aacttcttct
    30901 tggacgttag aacccagata gagttcattg ccgagttttt gacctgataa agcttcagct
    30961 agctcgctta ccatgaggac accgtagcgg ttatcccaag cttttgagat gatatttttt
    31021 tcattggctg tcaaaattgc agaactatct ggtacgatgg tatcaccagg acggatgcca
    31081 aaactttctg cctcagcctt gtccgcaaaa ccaccatcaa aaacgatatc ggcaatggct
    31141 ggcatggttg gtcccccctt tccacgagtc aaatgcggag gaacagaacc tgaaatcaca
    31201 ggaatttcat gaccatcacg agtcaagagt ttgaaacgtt ggctgctaac caccatgggg
    31261 ttccagccac cgatttctac gacacggaag gtaccatctg gcttgatttc gctgaccata
    31321 aaaccaactt cgtccatatg agaagcgacc aagacgcgcg gtgcatccac agcttctgaa
    31381 tgtttgatac caaaaatacc acccaagcca tctgtcacca cttcatccac atgcggtgtc
    31441 aacttttcac gaagataagc acggacaggc gcttcatgac ctgagactgc agcaagttct
    31501 gttacttctt taatttttga aaataatgtt gtcatttcaa ttccttcttt ctttcatcca
    31561 ttttaccact ttttatagga gaaggatagt gggaaggtgg atttctaagt tagtatctta
    31621 gtcctgctct atcttagaaa aggatagtat tctcttgcat gtagtgcaaa atctagtaaa
    31681 cattccaaaa ttaactcgaa tatttatttc caaacaaaaa aacaatacac catcaaagtt
    31741 gtttaggttt ttcatgaaat ttacagaaaa tagttgactt ccctttcttc tttctttaaa
    31801 t
//
LOCUS       DAVKJU010000020        30254 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVKJU010000020 DAVKJU010000000
VERSION     DAVKJU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.020"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..208)
                     /locus_tag="VW191_000850"
     CDS             complement(<1..208)
                     /locus_tag="VW191_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9623639.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKFSNKFGINNSNIRYMIKLIDR
                     YGIEFVKKGKNRYYSPDLKQEMINK"
     gene            complement(568..2538)
                     /gene="tkt"
                     /locus_tag="VW191_000851"
     CDS             complement(568..2538)
                     /gene="tkt"
                     /locus_tag="VW191_000851"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9623640.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            complement(2575..3270)
                     /locus_tag="VW191_000852"
     CDS             complement(2575..3270)
                     /locus_tag="VW191_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU9623641.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            complement(3281..4366)
                     /locus_tag="VW191_000853"
     CDS             complement(3281..4366)
                     /locus_tag="VW191_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEU9623642.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            complement(4392..4703)
                     /locus_tag="VW191_000854"
     CDS             complement(4392..4703)
                     /locus_tag="VW191_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEU9623643.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            complement(4731..5180)
                     /locus_tag="VW191_000855"
     CDS             complement(4731..5180)
                     /locus_tag="VW191_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9623644.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            complement(5158..5586)
                     /locus_tag="VW191_000856"
     CDS             complement(5158..5586)
                     /locus_tag="VW191_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9623645.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSGIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            complement(5579..7042)
                     /locus_tag="VW191_000857"
     CDS             complement(5579..7042)
                     /locus_tag="VW191_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9623646.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFKMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFSELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSAFLTEGDKQRLQAKIQEINYE"
     gene            complement(7571..9907)
                     /locus_tag="VW191_000858"
     CDS             complement(7571..9907)
                     /locus_tag="VW191_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEU9623647.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAHKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            10040..10789
                     /locus_tag="VW191_000859"
     CDS             10040..10789
                     /locus_tag="VW191_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU9623648.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(11081..11695)
                     /locus_tag="VW191_000860"
     CDS             complement(11081..11695)
                     /locus_tag="VW191_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEU9623649.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(12510..12779)
                     /gene="rpsO"
                     /locus_tag="VW191_000861"
     CDS             complement(12510..12779)
                     /gene="rpsO"
                     /locus_tag="VW191_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9623650.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(13058..13549)
                     /locus_tag="VW191_000862"
     CDS             complement(13058..13549)
                     /locus_tag="VW191_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU9623651.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVIGGAGS
                     LYIDETKMTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGN"
     gene            14407..14628
                     /locus_tag="VW191_000863"
     CDS             14407..14628
                     /locus_tag="VW191_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU9623652.1"
                     /translation="MELQELVERSWAIRQAYNELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            14788..16731
                     /gene="thrS"
                     /locus_tag="VW191_000864"
     CDS             14788..16731
                     /gene="thrS"
                     /locus_tag="VW191_000864"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU9623653.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(16780..17754)
                     /locus_tag="VW191_000865"
     CDS             complement(16780..17754)
                     /locus_tag="VW191_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9623654.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(17747..18424)
                     /locus_tag="VW191_000866"
     CDS             complement(17747..18424)
                     /locus_tag="VW191_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623655.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSIFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            18580..19374
                     /locus_tag="VW191_000867"
     CDS             18580..19374
                     /locus_tag="VW191_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEU9623656.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAHQIIVQSTALGGIAQHDTFS
                     WQIEDKHFVQLDKTNSDSRQVDTTFKEWVATVPDEELQLYFDLFFGTILDAGISSIND
                     LASLKALEYIHHLFVQAQSLTPEERETLGRLTQLLIDTRYHAWKNR"
     gene            complement(19465..19767)
                     /locus_tag="VW191_000868"
     CDS             complement(19465..19767)
                     /locus_tag="VW191_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623657.1"
                     /translation="MYLVIEIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(19836..20273)
                     /locus_tag="VW191_000869"
     CDS             complement(19836..20273)
                     /locus_tag="VW191_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU9623658.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(20349..21191)
                     /locus_tag="VW191_000870"
     CDS             complement(20349..21191)
                     /locus_tag="VW191_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9623659.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            21303..21953
                     /locus_tag="VW191_000871"
     CDS             21303..21953
                     /locus_tag="VW191_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU9623660.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLTLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            22134..22781
                     /locus_tag="VW191_000872"
     CDS             22134..22781
                     /locus_tag="VW191_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEU9623661.1"
                     /translation="MVVGIAIASGYLNSHLDKFVDWGSWTALVPFGLISVTNVGISML
                     STRFTGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQE
                     GRPNQMSQKQVKLAAIIISIIAFLFAFVTNYIGYGGKMNLLAYVTTIAFALSLIANAL
                     NALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVR"
     gene            22912..23325
                     /locus_tag="VW191_000873"
     CDS             22912..23325
                     /locus_tag="VW191_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623662.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            23344..23529
                     /locus_tag="VW191_000874"
     CDS             23344..23529
                     /locus_tag="VW191_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623663.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(23796..24239)
                     /locus_tag="VW191_000875"
     CDS             complement(23796..24239)
                     /locus_tag="VW191_000875"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEU9623664.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(24269..26491)
                     /locus_tag="VW191_000876"
     CDS             complement(24269..26491)
                     /locus_tag="VW191_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU9623665.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            27487..28116
                     /locus_tag="VW191_000877"
     CDS             27487..28116
                     /locus_tag="VW191_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9623666.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSYWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(28239..30131)
                     /gene="pepO"
                     /locus_tag="VW191_000878"
     CDS             complement(28239..30131)
                     /gene="pepO"
                     /locus_tag="VW191_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEU9623667.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         9029 a         6846 c         5514 g         8865 t
ORIGIN      
        1 ctttattaat catttcttgt tttaaatcag gagaatagta acgatttttt ccttttttga
       61 cgaactctat tccgtaacga tcaatcaatt taatcatgta cctaatatta gaattgttta
      121 tcccaaattt atttgaaaac ttctctaagc tatatccttg ttttctaagt tcatagatct
      181 gaactttatc atcataagtt aacttcatga taaaaacacc ccaaaagtta gattttttct
      241 gtctaacttt tggggggcaa ttcatcattt caacatccta tactatgcgt tttttaattt
      301 tagagacctt ctctctagct ttcaaaaaac ttacaatatc ctaagtcaat ctgtggatgc
      361 tgtgtttatt gattaaagaa actatctaga tcagtagcaa agtgattagc gtacttcgca
      421 accgtttcag ggtatactat ctactataac tttcgtagat aaattattac attctcatta
      481 tagtttttaa ataaccaatc atctgattag tgtctatcac tacatctgct agtctgacaa
      541 cagctgtatc ttcattgtaa ttggtttcta tagggactta acttgagcaa cgatattctc
      601 taccgtaaat ccataattat caatcacagt ctgagctggg gcagacgcac cgaagatgtc
      661 aataccgatg accgcgccat ccaaaccaac atacttgtac caactttggg tcgctgccat
      721 ttcaatggcc acacgacgac gagtcttaga tggtaaaatg tcttccttgt aggtagcatc
      781 ttgagcatca aatagttcgg ttgagggcat agataccaca cgtactttac caccttgtaa
      841 aaccaattcc ttagcagctt tgatagctag attgacctca gatcctgtag caatgataat
      901 agtatcaaat cccggggtat catacacgac gtaggctcct ttagcgacct taccaaagtc
      961 tgtcccttct tcaactacca agttttgacg ggttaagaca atgacagttg gagtggtggt
     1021 actggtcaag gcatgatgcc aagccgcttg agtttcacgg gcatccgctg gacggataac
     1081 agtcaagttt ggcattgagc gtaaacctgc caaatgttca actggttcat gagttggacc
     1141 atcttcacca acggcaattg aatcatgggt aaagacataa gttacaggca actcctgaat
     1201 ggctgatagc cgaatagcag ctttgacgta gtcagagaaa acaaagaagg ttccgccata
     1261 aactcgtaaa ccaccatgaa gagccattcc attgaggatt gttcccatgg caaattcacg
     1321 taccccaaac tgaatattgc ggtttaatgg attatattta tcttgtaagc catctgcctt
     1381 gatgtaggtc atgttggagt gagctaagtc tgccgatcca cctaagaagg ttggtaaaac
     1441 tgctgctgct gtattaatag catcttggga cgaattacga gttgcttgag agaagccatt
     1501 ctcatagaca gggaagtctt tttcagtaat ggttacaggg gattttccag ctacaatagc
     1561 gtcaatctca ctagcaactt cgggataagc aaccttgtaa tcagacacca aactagccca
     1621 agcatcgtat gcctcctgac cacgatccgc tacatttgtc ttgaaatcag aatatacttc
     1681 ctctggtact tcaaatggat cgtaatccca tcccaaaaac ttacgagttg ctcctgtttc
     1741 ttctgctcct agtggtgcac catgaacagc atttgtacca cttttattgg gtgagccgta
     1801 accaattacc gttttcactt caatcaaact cggcttacca gaaaacttgg ccgtctcaat
     1861 tgctgtagaa attgctgcta aatctgttcc atcttctacc agaactgtat gccaaccata
     1921 agcatcgtaa cgggcgcgaa cattttcaga gaaagtatct ttggtctcac catccaagca
     1981 gatgtcgttg gagtcgtaga ggacgataag cttatctaaa gcttgatgac ctgcataaga
     2041 agccgcctcc gcagacactc cttccatgaa gtcaccgtct ccagcgataa cataagtata
     2101 atggtcaaaa atagggaaac catctttgtt gtacttagca gctaaaaaac gctctgcttg
     2161 ggcgaaacca acggcagtag aaatcccctg accaagcgga ccagatgtcg catccacacc
     2221 agacgtatgc gtcacttcag gatgaccagg tgtcttagat ccccattgcc ggaaattttt
     2281 aatctcgtcc atggatacat cgttataccc tgttaaatgc aagagagcat acagtagcat
     2341 tgatccatga cccgcagaca agataaagcg atctcggtta atccagtttg gctgctcagg
     2401 tgtaattcta aggtgctttg taaataggct ataagccatt ggcgcagcac ccatgacgat
     2461 tcccgggtga ccagaattag atttgttaat agcatctacg ccaagaaaac gaatggcgtt
     2521 aactgattgt agtgacatat attcctccta actaataaat ttcttctcaa accattattt
     2581 gattggcatt gtttttccgg tcatttcttc gtaatctcta gaacagatat cccaggcttt
     2641 ggcaatatcg tcatccaaac caaataatcc actgcgacct ataacataaa tatctggtcc
     2701 tgccacatca atttgtttga aagtcttacg actcgaagaa ccatccatct caatgatgta
     2761 gtgataacca ttctgaactc taagctgacg gagttcttgg attttataca aggtagactc
     2821 caaaaagcgt tgtcctgcaa aacctggatc tacagtcata atagttactt tgtcaagtaa
     2881 atcaatgtag ggaaagattg tagaaacagg tgtttcagga ttaaggacaa caccagcctt
     2941 tagacctgca tcatgaattt tatcaatcaa acgaaaagca agaccattca gaacttcagc
     3001 atgaatacaa atatactcac attgtaaatc gagaacttga tctacccaaa aggttgggtc
     3061 tgtgaccatc agatgaactg ataaaggtgt gtcactaatt ttttgaactt cttgaatgaa
     3121 ccaaggagac aaggtaatat tgggaacaaa atggccatcc ataatatcga tatgataaga
     3181 tgctacttta tcattcaaaa aagtaatctg ctctttgaat ttgtccaaat ccatggtcat
     3241 caaagatggt gaaaattcaa ttcttgacat cgttaccctc ttacaaaatt tcaatatctg
     3301 acaaatcaat ctcttcttca acagtcataa ctggttctgt atgttttatt ggttttttca
     3361 aaatcgcgta gacaagtcct gtgactacaa tgttggctaa caaagcacaa ataccagcta
     3421 ctggacgagt catggttggt aacataagca ctccaccaaa tggcacggca gaatcagcac
     3481 ccattgtcaa agaaacagca ccaccaacag caccaccgat acctgttgca atgagacctg
     3541 gaaccaagtt attcataaca atcggaatta caccttcaac aatattgaca atccccatag
     3601 gaacagccga tttcaatgtt tcgatttcct cttgagtata gatatttttt ttgagtaatt
     3661 tcgcgataaa ataggccaat ccaaatccaa ctggtgtagc agtattcacc aattgcaaag
     3721 cagtcaatgg ttctttcaca ccttcagcct gtaaagtcaa cacaaaggca tagactgttt
     3781 tattaagtgg gccaccaaag tcaacagcac tgagaactcc aataactgcc cccatcaaac
     3841 catttgaagc acttcccaag ctttgtaata agctcgtcaa ccagttggta aaggctgcga
     3901 taggcgctcc aataatataa atcataatca aactacttac caaagaggct acaaaaggaa
     3961 taatcaaggt tggcattaaa cctttaatcc agtttggtac tttgaccttt ttaataatcg
     4021 cttgaaccaa gaaaccagct atataacctc ccaagatacc accgataaac cctgaaccaa
     4081 cagaattggc aattagacca acaacaaaac ctggtgcaat ccctggctta ccagcaatcg
     4141 agtaagacaa acctgtagca ataacaactg gcaagagacc aagggcttta ccacccatag
     4201 ttgctaaagc atcccagata gtgaattttc ctgctacaag agcgtcagga acaccacccc
     4261 ccattgctaa accaatggca actaagaatc ctgcaccaca aacaattgga atcatatagg
     4321 aaatggctgt caataagtga ccttttaagt ttaagtgttt caacatatat tttctcctta
     4381 aatattttca gttatttcgt aacaatctca acagctttag caatcagttt attgggagat
     4441 ttgactgcca cttctgttgg aaccttgata atctttttac cctcaaagcg ttccatacca
     4501 gaaatcttaa catcaactgc taaaataact acatccgctg catcaatctg ctcttgactc
     4561 aattcatttt ctacccctat tgtcccctga gtctcaacat gaatcacatg tccagctacc
     4621 tttgcggcat tctctaattt ttcctgtgca atataagtgt gggcaattcc cacagtacaa
     4681 gctgcaacac caacaatttt catacggata ccctccaaaa ttttttctta ttaacaaaaa
     4741 gctgcaatca catcatcaga tgtctgagcc cgaactaatt tggcaacaac ttcgtcatta
     4801 ccaagttttc gagcaaagag tgataaggtc ttcaaatgct ccctagcagc ttctgtatca
     4861 tcaccaactg caaagagtac aattactttg acccctttcc catcaatggt ctcccaagga
     4921 atctcattgt gatttatagc tatgactacc cccgccttct ccacagcaga actcttgcta
     4981 tggggaatag caatataatt cccaataccg gtctgtcctt ctgcctctct ctgataaaga
     5041 ccttcgataa attggtctct atcagacaca taacccgtct caaccaatag ttgagctaat
     5101 gcctcaaaaa cctcttcttt gctctgcatc tgtaaatccg tctggatcag actcacatta
     5161 agaatatctt tgatttccat atattatctc ccgtaattct tcttttgtta actgttttaa
     5221 ttgatttatg aatgattcat ctgctagtct tctcatcaat gttttaatac ctgacttgtc
     5281 ctgtgatact gcaatggcca aaccaataat aaggtcaaca cactggatat ccttcgacca
     5341 ttctctgata ggtggtttta atctagtaat cactaagaca tgatgttgaa agtttccttc
     5401 acaatgtggt agaagaacac ctttagcaac ctctatactt ccctgtctct cacggtaata
     5461 tagaagctct tctatttttt ctgtatcttc agaaacaaga aggctgattt gatttgctaa
     5521 ttctttgtag gcttcttgac gattttgaac agatatatcc ataaggacaa gcgaaagatt
     5581 attcatagtt tatctcctga atttttgctt gaagacgttg tttatcaccc tcggttagaa
     5641 aagcactaac taggacaaac gggacacttg ctggttcctg caaagctacc gtcgtcacaa
     5701 tgaaatctaa atctggatat agatttatca gctcatctaa ttgatgataa gcaactacat
     5761 caataatatc caattcagaa aattgctttt ctaatcgtgc tcgtaaaagc tctgaagttc
     5821 cgacacctga tgtacacatc actactgttt ttagaggacg tgctcgcttt tcttgaaacc
     5881 gtgcaaaata taagactaga aaaccaatct cgtccagact caaggacgca aaaccaaata
     5941 ctagactcac ttctttagaa atagttgtta actctttaac cagattagga taggttaaaa
     6001 gaatttgtga taaaagacta ttcttaatct gtactctatt atctaatctc ctcagtaagg
     6061 gactgatatg actcgccaag tcaacaaagt caggatctgt cgtttcaatc tctctattgt
     6121 ccatacacat acggctaaaa tagtaatgag tgacatctaa aactcgctga gaaaaaggaa
     6181 gcttcccgga agaaaatggt ttttgcaatc tcgaagatac aacgtattga taaagatagt
     6241 caatctcaac tgcatcaacc ttcatcttaa aatattgttc aatttcttga ataatttttt
     6301 gacagacact gtaaattttc tcatcaacaa tagtaggttt tgaaggtgct acaacatgaa
     6361 tactagtact acgacgattc ctactgataa aaatatacag gtgagagaaa atatttatat
     6421 tatatggata gggaatgttt acccccaatt ctctctcaag tgtaatcagt aatcccaaac
     6481 aaaagtgagc aagttctccg tcaagaggct gaaccttatt ctgtgtaatt tgctctaaat
     6541 ccaactgatt aaacattggc agtagattta gaatggctga acgaatttga gcctcatccc
     6601 catcaataaa aagctttcgt tgtctcattt ttaaatctaa cccataaatt gctagactct
     6661 cttgtaatat ctgacgatct tttagtacta ctgactcgct tacgtagaat tcctctccta
     6721 aatcataaat agaatgtggc ttaggagcag tcaacaagag acgttctagt aatttttcac
     6781 gccttacttc aggatcaaaa gcctctgtaa aattcccatc aacgtccacg agggaacttc
     6841 ttggatttaa tttgaagcct ctgccttttt cagataaaat gaatgcttct accggacaat
     6901 ctttgttgat tcccttgaca aggcgataaa ccgttttatc agagcaattt aatttttctc
     6961 ccaatacttt agcagatata taatccttac tatctcgcaa aaggagcaac aacgtttcca
     7021 tttgttcttg ttttctagac acgatacacc tcctttatga ttccattata tcgctttcaa
     7081 aaaataatat acttcgcatt ttgtcctttg caatggacat ttttgttttt atcaaaaata
     7141 tgatatcaaa tagaacagaa cctcactatg ccaaaatcat tttacctttt cttcataaca
     7201 tctattttag ttgaaatatt taagttgctt ttcgtatttg ataagaaata aaccaaacga
     7261 ttttctaata aataacacta tgttccattt aacaatagat agcatcttca ttttcaaact
     7321 accaacctat ccgaccattt gttctagcaa tagaagagag ttaaaattac ttcaatctgt
     7381 cgctttctct ttcttacatc aaacttcaaa tagtctaact acagatattt aaatctatta
     7441 cctcaatcca agcaggcttg agaatcactc ttgggttcac cgatagataa tagacatgta
     7501 ggccttgcac atcagatcta cgatagatcc atcgtataaa aaactgcaat ccacagattg
     7561 cagtctttct ttattttcta gcttgatagc gactagtcag gatgaaaatc actgtaaaga
     7621 ccaacatcat gacaccataa acaggcaacg tttgttctgt caaggttgaa atttcaagca
     7681 gtttttggat tcttgggaag agagctgtgg ctaggaagcc tcctactgac cagacaatca
     7741 agaggactcg ccatagagtg aatggcatgc aggctctaaa tacggataag aaaccaattg
     7801 accccaagag ataatagagg agagttgaga tttctaactc agaccaacct tgactcgttc
     7861 caaagatttt cacaaaaaga acgctaaaca cgaccatgag ggcacttggt agggcacgaa
     7921 gcatggattt tctgaggaaa tttggctcaa caggtttgat atttcgctca aaagtcagaa
     7981 cgaatggtgg gaaaccttcc acaaactggt caatcatggt aatctggatt ggaatgaagg
     8041 ggaaaatcaa gatccattca gaccgaccta gtaaagcact ggcgatacag ataactgcga
     8101 gcaagaatga gtagatggtc tttatcaaga aaatcggggc gatgtgggca atgttattga
     8161 ccacgcgacg accctcgaag agaatctcag gaacatcatt aaagtctgag ttcaagagaa
     8221 ccagattggc aatctgacgg gttgctggat ccccctccgc catcacgata gaacaatccg
     8281 cctcacgaag ggccaagata tcattaaccc cgtcccctgt catagccgtt gtatgacccg
     8341 cttttttcaa cgtttggatg atgagtttct tttgatgagg ggaaacacgt ccgaaaatag
     8401 ctgtctcctc cgccatggcc atcaattcct catcggtgat ttttgagcaa tctacatagc
     8461 tgtgatagtc cgcaaaacca gccttatggg caatgctgga caccgtaact ggattgtcac
     8521 cagagataat cttgagtccc acctcctgag aacggagata gtccagcgtc tctgctgctc
     8581 cctctcgaat ggggtccaag atttccagca aggctagagc ttgaatatca gatggtttct
     8641 gtggtttgtg atggtctaat ttctcctgac tgagagctaa gaccaagaca cgtgatcctc
     8701 tctccaaggc ctccctggct tctgggactt cagaatccag caacatctca ggtgccccta
     8761 agaaaactgt ccccaagcct tctaactcca tagcccccca cttgcggtcg ctcgagaagg
     8821 gaagattgga aatcatagga taagcaacat ctcccacaaa acgctggcga atggcttggg
     8881 cagttggatt cttatcctca ctatgggcca tgtagctagt caagatactg gcaatagcct
     8941 cttcaccata cgtttccgtc aacggaagaa cagcctccac ctgcatcttt ccttgggtga
     9001 tggtacccgt cttgtccaga cagagcatat ccacgcgcgc caaggtctca acagagtaca
     9061 tctcctgcac caagaccttt ttcaagccca acttaatcac tgcagtcaag agcgaagtaa
     9121 tggtcaaaag ggcaattccc ttaggcaaca ttcccaaaag agctgtcgac gagtttacaa
     9181 cggatgactt gagaggcagg ccttttaaaa gcaaggcttc cagcaagaga gccagaccaa
     9241 agggaatgat aatcttccca gtaaaaccag ccaacttgtc cagcgatttc atgatacggg
     9301 agttgatggg tttaacggtc ttagcctcaa gcatgagttt ggcagcatag ttgtctgcac
     9361 cgacatggtg aacttgagat aaaactgacc cactggctag gaaacttcct gacaaaagta
     9421 agccgtcaac ttccttttgc accaaatcac tttctcccgt taacatggct tcattgactt
     9481 ccgcaaagcc ttccaaaacc aaggcatcac taggaatctg ctctcctgca gacaaacgaa
     9541 tgacatctcc tagcactaat tcttcaggat taagagcaac ttcctgacca tcacggatgg
     9601 ttttgacctt ttccttggtc atgagattga gcttgtccac catgtgtttg gctcgtagct
     9661 cggtcacaat cccagaaaaa gcgttaaagc agataacagc aaagaagacc agattgctcc
     9721 aagcctgcac aaaggcaaga gccaaagcaa tggcaaagtt caaagcgtta aaaagggtaa
     9781 agacatttcg tttaacgatt tgccaagtgc tggtactggc tgatgcggta aagtcattga
     9841 ccaaaccctc agcctgtctt tccttgactt ctctttgggt taatcccata atcttatttt
     9901 tatccataaa ttctattata tcattttttt taacagaatt ctaatttcat cctaaatatg
     9961 tatctaatca agggaaaagt ttgccagccc tcctttgata aggtataata ataacaagaa
    10021 aacaatcgaa ggagatcgca tgttttatac ttatttgcgt ggattagttg tattgctctt
    10081 atggtccatc aatggcaatg ctcactatca taatactgat aaaattccta atcaagatga
    10141 aaattatatt ttagttgcgc ctcaccgtac ctggtgggat cctgtttata tggcctttgc
    10201 gaccaagcca aaacagttca tctttatggc aaaaaaagaa ctctttacca accgtatctt
    10261 tggttggtgg attcgtatgt gtggcgcctt tcccatcgac cgtgaaaatc ccagcgcctc
    10321 agccatcaaa tatcctatca acgttctcaa aaaaagtgac cgctctctca tcatgtttcc
    10381 aagtggtagc cgccactcaa acgatgtcaa ggggggcgca gcactgattg ccaaaatggc
    10441 caaggtccgt atcatgccgg ttacctacac cggtcccatg actttgaagg gcttgattag
    10501 ccgtgaacgt gtcgatatga actttggaaa tccaatcgat atctcagata tcaagaaaat
    10561 gaatgatgaa ggcattgaaa cagtcgccaa tcgtattcaa acagaattcc aacgtctgga
    10621 cgaagaaacg aaacaatggc acaatgataa aaaaccaaat ccactctggt ggtttatccg
    10681 catccctgcc ctcatccttg ctattatcct cgctatccta accatcatct ttagctttat
    10741 cgcaagcttc atctggaacc cagataagaa aagagaagaa cttgcataga agaaatgaac
    10801 cttggccaaa cagctaaggt tttcatttat atagtagatt ggaactagaa tagtacacct
    10861 ctacttctaa aacattttta gaaatcgatt tgactgtcct gatcgatttg tcctattctt
    10921 atttcatttt actatattta taagaatctc ctaatcaaat caaaagccac tctacaatag
    10981 agtaacttta agattgtcca tttatactca atgaaaaagc cctctgaaaa tcagagagct
    11041 gatttgagct cgagctaaaa tcctagtgaa acaaaaaaat ctacacgatc agaaaagtct
    11101 ctatcgtgcc attttcatac atgatgtata gtccaagtag aatgaatact aagggcacaa
    11161 tgattcgctc gtatttttca attgtctcga atattaacgg aatagaggat aacacccgac
    11221 taatctcgca aaagataatt atgccgatta caaacacaag caaggccacg agggtctgtg
    11281 accaatctaa cgaagcaaaa taaggtatat agatacctaa attatctccg ccagacgcaa
    11341 ttgtcagcaa tgtaactgtc caaaacagtt gatttgcctt gctttgttct aatctttcaa
    11401 taatttcttc ctcttcttct tccgcatctt ctccaacaat tgcaaagcga atccctaaat
    11461 agatagggat taaaccaagc aatccaacca tccattcttc aggcacgaaa ttaacgacat
    11521 aagcagcaac taaactcgcc cctacaagta agcctgtgcc tagatattgc cccgcataaa
    11581 tatgccattt ctgtttattc tgtgatagct gtgcaaataa aataattaaa ataattaaat
    11641 aatcgatact ggtggaaata taaacaccaa tagcagatat gattgtctgt cccataaaaa
    11701 aaactcctgt attagtcagt aataaatcag cagattttag gagagcacag acacattaat
    11761 ttagctatcg ctagtgggta atgtcgaacg taggaaagca ttttactcct cctcaaaacg
    11821 tagttacaaa gtaatttcta actggaattc agtgactact tccatgtaaa gtataacata
    11881 ctatacttta ctttgtaaag agtgggctct tctagggggc ttgcatacag ctactcggct
    11941 tgaaaagccg agtaactgcg cacctttatg gtgtaattag ctgataccgt ttgaggtttt
    12001 tgtagtctcc aaaattgtta ccgataaaac ctcaataaaa aagccctctg aagtcagaga
    12061 gctgatttga gcccgggcta aaatcctagt gaaaaagatg aaactccttg tgctcatcga
    12121 acactgtgtc atttccctat tttcatacgg atttttgacg cccttagcat catgattctt
    12181 gctagataaa tcgtttatag actaggcgac gaggcgaaga tcgtacgaaa ctttcagtga
    12241 aacaaaaaaa atctccccga agggagaaaa gctggtttat tttaccttac agtgctagga
    12301 accgtaacca aagattatac ttgagtatat cgaggtaagg tgacaacgca aaaaagctac
    12361 ccaatttaga gtagctttat tatcaagata tgctcaattg aacacggcct aagcacttgg
    12421 caaaaaagat aaaatctcct agaaactgaa gtttcttcgt caattttcct atttttgctt
    12481 tgtgcttttg acggcctttg tatcttgaat tagcgacgaa gtcctagaga gttgatcaac
    12541 tcacggtaac ggttaacgtc gtttttacgc aagtatgcaa gcaagttacg acggcgaccg
    12601 atttttttca tcaatccacg gtaagtagcg tggtcttttt tatgttgttt gatgtgttcg
    12661 ttaaggtgat tgatttccca agtaaggaca gcaacttgaa cctctactga acctgtatca
    12721 ccttcgtgac gtgcatattg tgcaatgatt tcattttttt tctcttttga gattgccatg
    12781 atgtttctcc tttttatttg gcttcatccg agtgacaggt tggcatgcct gtaaccaaga
    12841 agaagttatt tgtctttacg acaatcccta ttttaccaag atttaaattt tctgtcaaac
    12901 attaatactc tcatagttat tatttttcaa gtaaagagat acgttctttt atatgatgac
    12961 ctttttcaag ttcatctacc aatccaatag cataatctgc ataactaatc tggcttattc
    13021 cttttccatt agtagtaaaa atttctcctg ctagaattta gtttcctgtt ttttcaccat
    13081 cgggaataaa atctacagca ggactgacaa aagtccagtt gagattattt tttgtacgaa
    13141 gaagatccaa ttcatccgct tgagcttttg ctagtggctt aaattcttca ggaaaatcag
    13201 gcgtatctaa aagtcgtgtc atttttgttt catctatata aaggcttcct gctcccccta
    13261 taactagaaa acgtgtctgt gttccagcaa gaagctgatt aaatagttcg attgatttgc
    13321 tgtggagcgg tagcgtatct ggtgtataag caccaaacgc tgaaataaca gcatcaaatc
    13381 cagtaagatc atcttttgtc aactcaaata aatctttttt aataatagac tcagattgac
    13441 ttttgttttc agaacgaaca atagccgtta cttcatgtcc tcgtttgact acttcttcaa
    13501 caattgcttt ccccgcttgt ccatttgctg caataactgc tagtttcatt ttttatacct
    13561 ctcttgttgt aattatttta gttacagcaa ttgtgacact cttaataatc aatgtcaata
    13621 gtcttgctta attattatca aaatatttct accaagaaaa ctaaccatga ttctagtgaa
    13681 aagaaaaatc ttctttgtca acaaatttac tttcttgttt taaacatgct ataataatca
    13741 tagcaagaga tctaagttgt ctgttttttt aaaacgaggt gattatcatg cgtagattct
    13801 attcccatct cccctactat ctggtcatat tattctttta ttggccactt tatgagttgt
    13861 tcttgctagt tgtttctgac ccccttacac tatataaaca atcttctctt ctttacacct
    13921 ctggtaatct tgattgtatc gttactctat agctaccgtt tccgtttctc actttgatgg
    13981 ttagttggta acggactgct cttttacttt actatcataa cctttggtga gtttatacta
    14041 atttacttgc taatctatga aacagttgct ctggtgggca tggattctgg tattagcatc
    14101 aagcatattc tacaaaaaat gaaaaacaaa aaactttcac aaaatccttg aaaaatctca
    14161 caatcatgct ataataatcc atagagacaa gtcacttagt ccctttctac tagagagtgc
    14221 gtggttgctg gaaacgcata ggaagtctaa actgatacta ctcttgagtt ttttatgaaa
    14281 acataaaacg gtggccacgt tagagccaat cagaggtgtc cctctctttt gaggtacata
    14341 aatgaaggtg gaaccacgtt gcgacgtcct ttcgaggatg tcgcattttt tattaggata
    14401 ctaattatgg agttgcaaga attagtggag cgcagttggg caatccgaca agcttataac
    14461 gaactggaag ttaagcatca tgattccaag tggacggtag aagaagacct cttggcttta
    14521 tctaatgata ttggaaattt ccaacgactg gtgatgacaa aacaaggacg ctactatgat
    14581 gaaacaccct acacactgga acaaaaactt tcagaaaata tctggtgact attagaactt
    14641 tctcaacgtt tggatataga tattctgacg gaaatggaaa acttcctctc tgataaagaa
    14701 aagcaattga acgttaggac ttggaagtag tctgctgata aaaaatcaat gcttagaaac
    14761 tatgaaataa taaaaaagga gaacatcatg attaacatta ctttcccaga tggcgctgtt
    14821 cgtgaattcg aatctggcgt aacaactttt gaaattgccc aatctatcag caattcccta
    14881 gctaaaaaag ccttggctgg taaattcaac ggcaaactca tcgacactac tcgcgctatc
    14941 actgaagatg gaagcatcga aattgtgaca cctgatcacg aagatgccct tccaatcttg
    15001 cgtcactcag cagctcactt gttcgcccaa gcagctcgtc gtcttttccc agacattcac
    15061 ttgggagttg gtccagccat cgaagatggt ttctactacg atactgacaa tacagctggt
    15121 caaatctcta acgaagacct tcctcgtatc gaagaagaaa tgcaaaaaat cgtcaaagaa
    15181 aacttcccat ctattcgtga agaagtgact aaagacgagg cacgtgaaat cttcaaaaat
    15241 gacccttaca agttggaatt gattgaagaa cactcagaag acgaaggcgg tttgactatc
    15301 tatcgtcagg gtgaatatgt agacctctgc cgtggacctc acgttccatc aacaggtcgt
    15361 atccaaatct tccaccttct ccatgtagct ggtgcgtact ggcgtggaaa cagcgacaat
    15421 gctatgatgc aacgtatcta cggtacagct tggtttgaca agaaagactt gaaaaactac
    15481 cttcaaatgc gtgaagaagc taaggaacgt gaccaccgta aacttggtaa agagcttgac
    15541 ctctttatga tttcacaaga agtgggacaa ggtttgccat tctggttgcc aaatggtgcg
    15601 actatccgtc gtgaattgga acgctacatc gtaaacaaag agttggcttc tggctaccaa
    15661 cacgtctaca ctccaccact tgcttctgtt gagctttaca agacttctgg tcactgggat
    15721 cattaccaag aagacatgtt cccaaccatg gacatgggtg acggggaaga atttgtcctt
    15781 cgtccaatga actgtccgca ccacatccaa gttttcaaac accatgttca ctcttaccgt
    15841 gaattgccaa tccgtatcgc tgaaatcggt atgatgcacc gttacgaaaa atctggtgcc
    15901 ctcactggcc ttcaacgtgt acgtgaaatg tcactcaacg acggtcacct attcgttact
    15961 ccagaacaaa tccaagaaga attccaacgt gcccttcagt tgattatcga tgtttatgaa
    16021 gacttcaact tgactgacta ccgcttccgc ctctctcttc gtgaccctca agatactcat
    16081 aagtactttg ataacgatga gatgtgggaa aatgcccaaa ccatgcttcg tgcagctctt
    16141 gatgaaatgg gcgtggacta ctttgaagcc gaaggtgaag cagccttcta cggaccaaaa
    16201 ttggatatcc agattaaaac tgcccttgga aaagaagaaa ccctttctac tatccaactt
    16261 gatttcttgt tgccagaacg cttcgacctc aaatacatcg gagctgatgg cgaagatcac
    16321 cgtccagtca tgatccaccg tggggttatc tcaactatgg aacgcttcac agctatcttg
    16381 attgagaact acaagggggc cttcccaaca tggctggcac cacaccaagt aaccctcatc
    16441 ccagtatcta acgaaaaaca cgtggactac gcttgggaag tggccaagaa actccgtgac
    16501 cgcggtgtcc gtgcagacgt agatgagcgc aatgaaaaaa tgcagttcaa gatccgtgct
    16561 tcacaaacca gcaagattcc ttaccaatta attgttggag acaaagaaat ggaagacgaa
    16621 acagtcaacg ttcgtcgcta cggccaaaaa gaaacacaaa ctgtctcagt tgataatttt
    16681 gttcaagcta tcctagctga tatcgccaac aaatcacgcg ttgagaaata agagtctagc
    16741 ataaaagcct ccaatctgga ggctttttct catctatttt tactcaagga ctaagttcac
    16801 ttgagcaaac tgaatccgca ctgtcgttcc tttcccgacc tcagactcga tacgaatctg
    16861 atgccccagt tcttcagaaa ttttcttaga tagataaagg ccaagtccag aggactgctg
    16921 ggtcaaacgg ccattgtatc ctgaaaagcc acgttcaaat actcggagaa catcactgtt
    16981 ttttatcccg attcccgtat ctttgataca aagctcttgg tcatccatat aaatctccag
    17041 accaccttcc ttggtgtact tgagactgtt tgagatgatt tgctcaataa ccactagcag
    17101 ccacttttta tccgtcacga tttctttatc aaggtcatgt agattgacat ttaagccttt
    17161 ttgaataaag aaaagagcat atttacgaat tatttccttg accaagtcct caatttgaac
    17221 ctgctttaag agcaaatcat catggaaact ttctaaacgc aggtactgta aaactaggtt
    17281 ggtataggag tcgattttga aaatttcctg ttctagctgc tgcttcagtt ggcggtcgac
    17341 cacttctgca actaagagtt gactggctgc aatgggggtc tttatctgat ggacccacaa
    17401 ggtatagtaa tccagcaaat ccgtcagttt tctttctgct tttgacctct gctgatagag
    17461 ttccatctca cgcgcttcta atttttctgc taaagctatt tccaaaggag acttggcttc
    17521 cctctctcca tagagaagtt cctggcgata gacctgcatt tccaccaata tgtcccaagt
    17581 gaaaaataag atggttacaa agcaacacaa gaggaaaaag tagaggaagt aaattcctag
    17641 actggcaaat aaaaactgaa agagtaagac cagaaatgcc aaagaaagca gatagacaaa
    17701 aagacgacta cgggagcgca gataggctag aaaaaattgt ttccaatcaa gcatgcttca
    17761 atccgtaccc tattcctttc ttggtctcga taaatcctac caatccctgc tcctccaact
    17821 ttttacgcaa acgagccaca ttgacagaga gggtattatc atcaatgaaa aagtcactgt
    17881 tccaaagttc tcgcatcagg tcgtcacgtg ctacgatgtt gcctgcatgc tcaaataaca
    17941 cgcgtaaaat ctggaattca ttcttggtca aattcaagac ttgcccttga taatgtaaat
    18001 ccatggattt ggtattgagg ataacaccag catattccag caaactctca tcacgcccaa
    18061 actcatagga acgacgcaac aagccctgaa ccttagctaa aagaacctgc tggtcaaaag
    18121 gcttggtcac aaagtcatcc gcccccatat tgattgccat gacaatatcc atagcctggt
    18181 ctctcgaaga aagaaacatg ataggtacct tggaaatctt gcggatttcc tggcaccagt
    18241 gataaccatt aaacaagggc aaaccaatat ccatgaggac cagatgaggt tccgactgaa
    18301 caaatatact caaaacttcc ataaagtctt ctaccaggac cacttcaaat ccccattcag
    18361 agagcatttt cccaatctgt tgacgaatga cctgatcatc ttctattaat aaaatcttgt
    18421 gcatacgctt ctccttttcc attattataa cagatttttc catgctagat ggtctgaaac
    18481 tgaatttgaa atagcctgtt tttagccagt acaaacaggc tatgctacta gctaatttga
    18541 gggaaatttg ctaagataaa taaaaagaaa ggagctctta tggccaatat ttttgactat
    18601 ctgaaagatg tcgcatatga ttcttattac gaccttccct tgaatgagtt agacattcta
    18661 accttaatag aaatcaccta cctctccttt gataatctgg tctccacact tcctcaacgt
    18721 cttttagatc tagcacctca ggttccaaga gagcccacca tgcttactag caaaaatcgc
    18781 cttcaattat tagatgaatt ggctcaacac aagcgcttca aaaattgcaa actctcccat
    18841 tttatcaacg acatcgaccc tgaactgcaa aagcaatttg cggctatgac ttatcgtgtc
    18901 agcctcgata cctatctgat tgtctttcgt gggacagatg acagtatcat tggctggaag
    18961 gaagatttcc acctgaccta tatgaaggaa attcctgctc accaaatcat cgttcagagt
    19021 actgccctgg gtggcatcgc ccagcacgat acctttagtt ggcagattga ggacaagcac
    19081 ttcgttcaac tggataagac caacagtgat agccggcaag tagacacaac ctttaaagaa
    19141 tgggtggcca cagtccctga cgaagaactt cagctctact tcgacctctt ctttggcact
    19201 attcttgatg ctggtattag ctctatcaat gacttggctt ccttaaaggc gcttgaatac
    19261 attcatcatc tctttgtcca agctcaatcc ctcactccag aagaaagaga aaccttgggt
    19321 cgccttaccc agttattgat tgatactcgt taccatgcat ggaaaaatag ataatactct
    19381 tgaaaattaa atgtatacaa aacaaaagac ctagaataca tactttcatg tgcattctaa
    19441 gtctttttaa atagaatcta atagtcaata aaaatcaaag agcattgaga gataatgggg
    19501 cttggaacgt ccctctcgtt tcaacaaaat gaccccatta tagattaaaa agatgccact
    19561 tagaaaaagc aaaaaaggaa gtaagacaaa ggcaaataca taaaaagcta actgaacatt
    19621 ctcgtatcca tttttataaa aaaggtagga tagataaaaa taacttgaaa tgagggataa
    19681 taaaaataat actggattcc acaaacttct attatccttc caaaatgaca ctataaaggc
    19741 taatacaatt tctataacga gatacatttc ttactccttt aatagctaca ttttatcata
    19801 attatccaaa gaaaaaaaga gggcatttat ccctcttaat ccttcatctg actctctgca
    19861 tcggccacga ctttttctag actggtctga ccaagttctg cctccatagt caactgaatt
    19921 ctctccaatt tttgatccaa aacatcatga atatgagctc ctacagggca atttggattc
    19981 ggattgtcat ggaaactgaa gagttgacct gtcttaccaa gacattcgac cgcctgataa
    20041 acatctaaaa gactaatatc cttaaggtcc ttgacaatct ctgttccgcc cgttccacgc
    20101 gctactgaaa tcagctctgc cttcttcaac tgggacaaga tctttctgat aatgacagga
    20161 ttgaccccaa cactagcagc cagaaaatca ctggtcacct tgctttcctt cccctcgagg
    20221 gcaatgatta tcagcatatg agtcgcaatg gtaaatctac ttggaatttg catcctcttc
    20281 tcctttttac gaggctaccc tgcctctact cttctttttc tattattata ccctttttag
    20341 ttgtaatgtc aatcgttacc acttttcaac cagtcgtcta actcccgatc gcaaccctct
    20401 ttctgagcca attctctcaa aaattcctga tgatgagtat ggtggatccc attgaccaga
    20461 ctttcatagt aaacctcaaa atagggaagt ctcaggtctt tagccagctg caattcagct
    20521 gctacatcgt agtctacccg tcggaagtcc atatctacca ggcctttgtc atcaaactcc
    20581 aaaatcatat actgggcccg caagtccttc cgtagctgag cgtccaaaaa gaaaggttgg
    20641 ccaatcgaac ccggattgac aatcaattgc ccaccagtcc cgtaacgaag caactgctgg
    20701 tgaatatgtc cataaacagc aatatcacag ggaggatgag tcaccaagcg gtcaaactcc
    20761 tcttgtttgc cagtatgaat caactctcgc ccccagttct tatcaggcag atgatggcta
    20821 attcccaccg tcaaatcccc aaactgacga tgaatttgaa gaggttgatt gtggagcact
    20881 tcaatttctt ctagggaaat ttcctctaaa acatactggc actggcgcaa gagatagcgt
    20941 tgactggggc gagtactatc caattcctta cggacaccat gccaaagact gtcttcccag
    21001 tttcccaaaa ctctagccgt aatcggtagt tgatccaaca agtccaaaat ccttctacgc
    21061 cctgtccctg gcatgagaat atctcccaaa agccagtatt catccactcc tatctgccga
    21121 gcatctgcca aaacagcctc caaggcggtg gtatttccat gaatatctga aagaagagct
    21181 attttcgtca tatccatctc ctcgtttttt ctcttgcaat aagtataaca taaaaagtca
    21241 cagctagaga aatctagctt tttttgatat actagataaa gatattagac aagaggaaac
    21301 gaatgacccc aaacaaagaa gactatctaa aatgtattta tgaaattggc atagacctgc
    21361 ataagattac caacaaggaa attgcggctc gcatgcaagt ctctccccct gccgtaactg
    21421 aaatgatcaa acgaatgaaa agtgaaaatc tcatcctaaa ggacaaggaa tgtggctatc
    21481 tactgactga cctcggtctc aaactggtct ctgagctcta tcgtaagcac cgcttgattg
    21541 aagtttttct agttcatcat ttagactata caagtgacca gattcacgag gaagctgagg
    21601 tcttggaaca cactgtctct gacctgttcg tggaaagact agataaactg ctaggtttcc
    21661 ctaaaacctg cccccacgga ggaactattc ctgccaaggg agaactcctc gttgaaatca
    21721 ataacctcac actagctgat atcaaggaag ctggcgccta ccgcctgact cgggtgcacg
    21781 atagttttga cattctccat tatctggaca agcactcact tcacatcggt gaccagctcc
    21841 aagtcaagca gtttgatggc ttcagcaata ccttcactat cctcagtaac gacgaggatt
    21901 tacaagtgaa tatggacatt gcaaaacaac tctatgtcga gaaaatcaac taatttctca
    21961 agtcccctac caaccctgaa agttttattt tcagggtttt atttctatca tttgatttta
    22021 ctttcatttg gtggtagagt atttgaaaaa aaaaaagaaa gatagtttaa cctatgaaaa
    22081 aatcactaaa aatttttgct acatctaaat agtttgacct cttcggggtt gctttggtcg
    22141 ttgggattgc gattgcatct ggttacctca actcacacct cgataaattt gtagactggg
    22201 gatcatggac agctcttgta ccctttggat tgatttccgt aaccaacgtt gggatttcca
    22261 tgttgtccac tcgtttcacg gggaaattaa gcaaatgggg aaattacttt ggtattgtta
    22321 ataccatttt gtccggtgct attgattata tccttggaaa taaggcggcc atcattacct
    22381 atcccgtcac cttcctcatt tatacctttg ctataaagaa gtgggaagct tcgcaagaag
    22441 gcagacccaa ccaaatgagc caaaaacagg taaaattggc ggccatcatc atttccatca
    22501 tcgccttcct ctttgccttt gtgaccaact atatcggata tgggggcaag atgaatctcc
    22561 ttgcctacgt aacaactatt gcctttgcac tgtccctcat tgccaatgct ttgaatgcat
    22621 taaaactaac aactcagtgg ggcttttggt tgatttacaa tttcgttcag ctgacaaaag
    22681 ctggtattca aggaaacttc gccaatatcg gtaaatacat cttttatatc ctcaatgcaa
    22741 tcggagcttt atttgtctgg aatgatgaag aagttagata atagaaggaa actcaaataa
    22801 agaatactag ccaacttaag agaatctccc ttctgctatt tttatcattt tagttgtaac
    22861 tcccattctt ttggagtaga atgaagttag ataaaagagg gaggtcttgt catgaaaaaa
    22921 ctctatagaa tccattttat agcaattgca gttattgatt tgctactatt tgcatttttt
    22981 atcactagac tcgagacatc ttttgagtgg ctcttgctct ctggtttaat tttcttcctt
    23041 gctcaaggac tcctgctatt tctcttggtc gtccgactca aacatcaatt cgctgagatt
    23101 tatcctcaaa tcaataaaaa gattcgcttc tactatttag gggttctcac cattgatttt
    23161 ctattttttg ttctcttagc cttcattagt tctcagcgtt tttcatctct tatgccaatc
    23221 atcactgctt gccattctac tttttattat atgacagctg actacctaag agaaaactat
    23281 ccagactttt acgacaaaca catctcttta tgggagtgtc tctaaggaaa aggaggtttt
    23341 agcatgaaaa aaatcatctt catcaaaacc attcaactcc ttgtcattga tggaatcatg
    23401 ctggcatttt tgacatttaa aagggggctt acttgggact ggattttgat ttatagcggt
    23461 tggctcattt tctttcatcc tgtgctattg acctatcttt caaaccaact ttgtgaccac
    23521 tttagttaac tctattccca gattagaccg agattctggc gttttgcttt acaaattctc
    23581 ctatgggata gcctgatgat tctctccttg gtgtctttaa gtgatattcc acttttcctt
    23641 cagggaactc tcctcatcct aggacatctc atcccttcct atcgcatctg ccaaagcctg
    23701 aaaagagact tcccccaagc atatcaagaa ccgatttctt tttggagtat tttatgatcg
    23761 atgagaaaga ccaagccgac tgggcttggt ctttcttatc tctttttagt atctaggata
    23821 atggtaacag gtccattatt aaccagctca acctgcatat ctgctccaaa gatgcctgtc
    23881 tgaacgggca cttcttgcgc taatttttga ttgaaagcat catagaagtc tgatgccata
    23941 tcaggtttag ctgcccctgt aaaggctgga cgattgcctt tcttagtatc cgcaaagagg
    24001 gtaaactgag aaatagagag gatttctcct tcaatatctt tgacagacag gttcatcttg
    24061 ccttctgcgt ctgaaaaaat ccgcatattg accagttttc tcacagcata gtccaaatct
    24121 tcctcttggt cctctggtcc aacaccaacc agcaataaaa gtccctgatt gatttttccc
    24181 tgaatctggc cttctatact cacttgggct tttttaaccc gttggataat gattttcata
    24241 atagcctttc tagtaagagc taggacaact agccgttggt ccgtttgaca gagtaaactt
    24301 ctggcacact cttaatttta tcgacaaccg tggtcagtgt agagaggttg gcaataccga
    24361 aggacacatg gatattagca aacttcatat ccttggttgg ttgggcattg accgttgaaa
    24421 tattcttggt tgtatttgaa agaacttgca gtacatcgtt caacagtcct gtacggttga
    24481 gaccgtagat atcgatatgg gccatatact ccttatttga gctagagtac tggtcttccc
    24541 attccacatc aaggagacgt tgctcgtagt tttcttgggc acgcaggttc atacagtcca
    24601 cacggtgaat agccacacca cgacccttgg taatgtagcc aacaatatcg tcaccaggca
    24661 cggggttaca acacttagca atccgcacta ggagaccaga agcaccttca ataaccactc
    24721 ccccctcatg cttgaccttg agggtttctt tattttcaac cttgacctcg ccacctttga
    24781 caagctcctc tgcctcagct ttggccttgg cacgctcttc ctcacggcgt tccttttcag
    24841 tcagacggtt aaagacggta atcgcaccga tttccccaaa accaatggcc gcaaagaggg
    24901 agtcttctgt cttgtaactg gtcttttgca gaacttgatc catgtggcgc ttgtccataa
    24961 atttatttgc cacatagcca ttttcttgga actgagccat cagcatctca cgacccttgt
    25021 tgacagacaa ttccttatct tggtttttaa agaactggcg aatcttattg cgcgccttgc
    25081 tagtcttgac catattgagc cagtcacggc taggtccaaa ggagttcggg ttggcgataa
    25141 tttcaacctg atcccctgtc tttaacttgg ttgtcagtgg aaccatgcgg ccattgacct
    25201 tggcaccagt tgctttttca ccgaccttgg tatggatttc gtaggcaaaa tcaatcggtc
    25261 ctgaatcttt gggaagggaa cggacagctc catctggggt aaaaacgtaa atctcctcag
    25321 ccaaatagtt ttccttaaca gagtccacaa attccttagc atcatcagcc tggtcttgga
    25381 gctccatcat ctccttgatc cagttcattc caatagctga ttccttgctg ttaacttgcc
    25441 cctttatacc tttcttataa gcccagtgag ccgcaacccc gtactcagcc acctcgtgca
    25501 tttccttggt tcgaatctgg aattcaatcg gcccttttgg tccataaaca gtcgtatgga
    25561 tagactgata accattggcc ttgcggttgg caatatagtc tttgaagcga cctggcatcg
    25621 gtttccaaaa ttcatgcacg taaccaagca tggcataaac atcactttgg gtatctaaaa
    25681 tacaacgaat agcaatcaga tcatagattt cctcaaaccg ttttctcttg tcctgcattt
    25741 tgcggaaaat tgagtaaata tgcttgggac gaccataaat cttccctttc aagtgacgtt
    25801 ctgtcgtata ctcctctaat tttgtgacta cctcatccac caaggcctca cgctccctgc
    25861 gcttttcctt catcatatgg gtaatcttgt aaaactccgt tggattgaga taacggaaag
    25921 acaagtcttc taattcccat ttgacactgg aaatccccaa acgatgggca agtggggcat
    25981 agatttccat ggtttctttg gaaatacgct cctgcttgtc ttttcgaaga tgtttcaggg
    26041 tccgcatatt gtgcaagcgg tcagacagtt tgaccaaaat aacgcggatg tcctcagaca
    26101 tggccatgag catcttgcga tgattttccg ctaattgctc ctcgatcgat ttgtactcga
    26161 ccttgccaag cttggtaact ccgtcaacaa tcacccgcac atcaggacca aactctcttt
    26221 ccaaatcgtc caaggtcgca tctgtatctt ccaccacatc atgcaagaat ccacaagcta
    26281 ctgttacagc atccagcttt agcttagcta aaatacctgc cacttggata gggtgaatga
    26341 tataaggctc gcctgatttg cgatattgac cactgtggca ttcaacagca tagaccaagg
    26401 ccttatggac aaaatgaaca tcctcttccg ttaaatattc tttggttaaa gcgacaactt
    26461 cttcgcctgt taaattcact tctttcggca tctctactct ccaattcttc ctaccatttt
    26521 atcacttttt taagaatatg aaaactagat tggaacagaa taagaaaaaa ataattcaaa
    26581 attgcttgat aattctgaat tattggccca taatatacta cgaagttaga ttttaaactt
    26641 aggtgataga aggagagata gaagaacgga aaccatattg taacccaaag actttctgac
    26701 ttccccaatt ccattgaaga tacgaaagat aaacggtgga actcgtatca catacactgg
    26761 taccttgact ggattttaga attaatactc aatgaaaatc aaagagcaaa ctaggaaact
    26821 agccgcaggt tgctcaaagc actgctttga ggttgtagat aagactgaca aagtcagtca
    26881 catatatacg gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtataaaaat
    26941 cctcaagata ctttcttcta tcctttagtt tataaggaga atacctatga aaaaaactgc
    27001 tatttctatc tttgctctcc taatgttagg agtttgctgc ctgttcctat tcagccagca
    27061 aagctataaa aaacagtcgt tcaatactat gctaacgacc agaacctacc cagtaggata
    27121 acttatagtg aatatagcga caaatgagaa gccaactacg gtagcactct aaacatcacg
    27181 tctatcaaac aagctaatga cggagtttat gcaacctatg aagggcaatt gacacctttc
    27241 caatattgat aaattgataa ccagcctgtc ttcatctagt catgctggtt tttaagttca
    27301 ttttaaatcc ttacctattc tccctaactg tgctatactt aatttatact caatgaaaat
    27361 caaagagcaa actagaaagc tagccgcagg ctgttcaaag cactgctttg aggttgcaga
    27421 taaagttgac gcggtttgaa gagattttcg aagagtatta gtacattctt tgagattgga
    27481 gctagtatga aaatccataa aaccgtgaat cctgttgcct atgaaaatac ctattatcta
    27541 gaaggcgaaa aacacctcat cgtcgtcgat cctggtagtt attgggaagc cattcgtcag
    27601 acaatcgaga agatcaacaa accgatctgt gctattctct tgacccacgc ccattatgac
    27661 catatcatga gtctggactt ggttcgcgag acgtttggca atcctcctgt ctatatcgca
    27721 gagagcgaag ccagctggct ctacactcct gtcgataatc tatccggtct ccctcgccac
    27781 gatgatatgg cagatgtggt cacaaaacct gcagaacaca cctttgtctt tcacgaagaa
    27841 taccaactag aggaatttcg ttttaaggtt ttaccgaccc cagggcactc tatcggtggt
    27901 gtttccctag tctttcctga tgctcaccta gtcttgacgg gagatgctct attccgcgaa
    27961 actatcggac ggaccgacct tccgactggt agcatggagc aactccttca tagtatccag
    28021 acccaactct tcaccctacc aaactacgat gtctatccag gacatggtcc agctactact
    28081 atcgctcacg aaaaggcctt caatcccttt ttctagcaag atgatgacaa tcgaaattta
    28141 agtaaactat ccagcaaatc tttctattac aaaaggcatc ctatcaaggt tttcacacgt
    28201 gattggatgc cttttctctg atgactagat tttttgtatt accaaataat cacgcgctcc
    28261 tctggtgaac gccacattcc gtctccttct ttgacatcat aggttgtaaa gaaatcgtcg
    28321 aagtttggta cttgcacatt gacacggagt ttggctggtg cgtgcacatc gacgctagcc
    28381 aaaagtttca taaattctgg acgacctttc atgcgccaga tgcgaccgaa gttgtagaag
    28441 aactcttctg ctgagaagtc tgcttctctc ttagctgctt caagcgctgc tgcgattcct
    28501 cccaagtcag ccacgttttc tgatacagtc aatttaccgt taatggttgc tccataagaa
    28561 tcctgtccat caaattggtc aatgactttt tgtgttttct ccttgaaggc agcatagtcg
    28621 ctctctgtcc accaatcctt gagactacca ttttcgtcaa aggaagcccc gttagtatca
    28681 aaggcgtggg aaatttcatg ggcaatcact gccccaatac caccgtagtt agcagaagat
    28741 gactgatgca agtcatagaa aggcgcctgt aaaatggccg ctggaaagac aatcaggttc
    28801 ttctgaggat tgtagtaggc attgaccata tgagcaggca tgccccattc cttatagtct
    28861 acaggctggt tccacttact ccaactgtgc ttgatttcca cacgcgcaaa ggctagagca
    28921 ttctcaaaaa gactggcagt ttcattcact accttatcct tgtaacgtgc aggcaattct
    28981 tctggatagc caatataagg tttgatcaca ttgagcttca cgatagcctg tttacaggtt
    29041 tctggagtga gccagtcatt cttaagcaga cgctccttat agacatcaat catggttgcc
    29101 acttttttct ccacatccgc cttggcttct ggagagaact tctcacgggc gtaccaaagc
    29161 cccagggctt gcttgaaagg ttcctgtgct agatgataag ctgctttgac cttatctttt
    29221 gcctctggaa ctccagaaag ggcacggctg taggcaccag acaaaacacg gatatcctct
    29281 gttaaatagc tggttgaaag attgacaaca ctcaaaatca aggttgcttt aaggagagac
    29341 caggcttcct cactgtaaaa ttgctctgct gcttgccaga aacgttcctc gtctacaata
    29401 accttgtctg gtaattgccc aataactgct ttgaagaagt catccaaagg tagggcaggc
    29461 gcgaatttct tgaaatcttc gtaagaatat ggatgataga gtttagcata ttctgaactt
    29521 tcttcattag agagcaccac tgccgcaact cggcggtcca attcaagtct tttttctagc
    29581 aagtcttcaa tttcttcatc agagaaatca taagccttga ggagatttgc gctactttct
    29641 ttccaaagag tcaagagctc ttcgcgctga ggatgttctt ctgcatagta ggtcgtatct
    29701 ggcaagattg tgcttggagc gctagcccat agaacattga ttctagcatc cataaagtct
    29761 ggcgatacac caaaaggaag gaagtttggt tttcctgcaa gctcaaactc tgctagttta
    29821 gctgtaaaat ccgcaaaagt ttccaattct tggaattctt taaggagtgg taagacaggt
    29881 gtgataccgt cagcttctct cttgtcaaaa tcacgaacta ggcggtggta tttgacaaag
    29941 ttttccaaga tagcatcctc aggcacttct tcacctgcta accacttgtc tgttgtcgcc
    30001 agcatcaggt cttcaatttc ctggtctaaa tcaacaaaac ctcctgtttg agacttatct
    30061 gctgggattt cagctgtctg ttgccattct ccattgatag catcataaaa atcatcttga
    30121 taacgtgtca tcttgttctc gctttcattt gtatttgcat ttatcttaac aaaaatcact
    30181 agggaatgca attaataacc atttaactat tattttttag ctattataaa aaacctttcc
    30241 atgataaaac gcat
//
LOCUS       DAVKJU010000021        29791 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVKJU010000021 DAVKJU010000000
VERSION     DAVKJU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29791
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.021"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            132..890
                     /locus_tag="VW191_000879"
     CDS             132..890
                     /locus_tag="VW191_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9623668.1"
                     /translation="MVLVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDRMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            930..1694
                     /locus_tag="VW191_000880"
     CDS             930..1694
                     /locus_tag="VW191_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEU9623669.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTNKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1707..2384
                     /locus_tag="VW191_000881"
     CDS             1707..2384
                     /locus_tag="VW191_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9623670.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGATLLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2394..3053
                     /locus_tag="VW191_000882"
     CDS             2394..3053
                     /locus_tag="VW191_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9623671.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3311..4501
                     /locus_tag="VW191_000883"
     CDS             3311..4501
                     /locus_tag="VW191_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEU9623672.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(4822..5703)
                     /locus_tag="VW191_000884"
     CDS             complement(4822..5703)
                     /locus_tag="VW191_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEU9623673.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(5842..7170)
                     /gene="vncS"
                     /locus_tag="VW191_000885"
     CDS             complement(5842..7170)
                     /gene="vncS"
                     /locus_tag="VW191_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEU9623674.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKWVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(7167..7823)
                     /gene="vncR"
                     /locus_tag="VW191_000886"
     CDS             complement(7167..7823)
                     /gene="vncR"
                     /locus_tag="VW191_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEU9623675.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(7872..7955)
                     /gene="pep27"
                     /locus_tag="VW191_000887"
     CDS             complement(7872..7955)
                     /gene="pep27"
                     /locus_tag="VW191_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEU9623676.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(8135..9514)
                     /gene="vex3"
                     /locus_tag="VW191_000888"
     CDS             complement(8135..9514)
                     /gene="vex3"
                     /locus_tag="VW191_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEU9623677.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(9566..10213)
                     /gene="vex2"
                     /locus_tag="VW191_000889"
     CDS             complement(9566..10213)
                     /gene="vex2"
                     /locus_tag="VW191_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEU9623678.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(10226..11503)
                     /locus_tag="VW191_000890"
     CDS             complement(10226..11503)
                     /locus_tag="VW191_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623679.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(11861..12031)
                     /locus_tag="VW191_000891"
                     /pseudo
     CDS             complement(11861..12031)
                     /locus_tag="VW191_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(12174..12773)
                     /locus_tag="VW191_000892"
     CDS             complement(12174..12773)
                     /locus_tag="VW191_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623680.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            complement(12910..13794)
                     /locus_tag="VW191_000893"
     CDS             complement(12910..13794)
                     /locus_tag="VW191_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEU9623681.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(13798..14184)
                     /locus_tag="VW191_000894"
     CDS             complement(13798..14184)
                     /locus_tag="VW191_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEU9623682.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(14177..15520)
                     /locus_tag="VW191_000895"
     CDS             complement(14177..15520)
                     /locus_tag="VW191_000895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU9623683.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(15602..16486)
                     /locus_tag="VW191_000896"
     CDS             complement(15602..16486)
                     /locus_tag="VW191_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623684.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARVLFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDKYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(16498..17115)
                     /gene="cysE"
                     /locus_tag="VW191_000897"
     CDS             complement(16498..17115)
                     /gene="cysE"
                     /locus_tag="VW191_000897"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEU9623685.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(17131..19344)
                     /gene="pnp"
                     /locus_tag="VW191_000898"
     CDS             complement(17131..19344)
                     /gene="pnp"
                     /locus_tag="VW191_000898"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU9623686.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(19903..20769)
                     /gene="metF"
                     /locus_tag="VW191_000899"
     CDS             complement(19903..20769)
                     /gene="metF"
                     /locus_tag="VW191_000899"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU9623687.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(20833..23082)
                     /gene="metE"
                     /locus_tag="VW191_000900"
     CDS             complement(20833..23082)
                     /gene="metE"
                     /locus_tag="VW191_000900"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU9623688.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLSAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVVAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(23343..23609)
                     /locus_tag="VW191_000901"
     CDS             complement(23343..23609)
                     /locus_tag="VW191_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9623689.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(23693..24181)
                     /locus_tag="VW191_000902"
                     /pseudo
     CDS             complement(23693..24181)
                     /locus_tag="VW191_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            24337..25338
                     /locus_tag="VW191_000903"
     CDS             24337..25338
                     /locus_tag="VW191_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEU9623690.1"
                     /translation="MIKTFLSALSVILFVIPIIAYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSDILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            complement(25406..27808)
                     /locus_tag="VW191_000904"
     CDS             complement(25406..27808)
                     /locus_tag="VW191_000904"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU9623691.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVGSPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEERQIVCGAPNVRAGIKVMVALPGARIADNYK
                     IKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDDE
                     IIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDKA
                     PYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDTF
                     EGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKSS
                     RVVLEAAVFNGKSIRKTSGHLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGAT
                     VRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFTV
                     SVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVIQKLRRQVRTIAEG
                     AGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNVA
                     RKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYAK
                     GILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIPE
                     TYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVKR
                     LTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNAE
                     VR"
     gene            complement(27885..28394)
                     /locus_tag="VW191_000905"
     CDS             complement(27885..28394)
                     /locus_tag="VW191_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623692.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(28394..29440)
                     /gene="pheS"
                     /locus_tag="VW191_000906"
     CDS             complement(28394..29440)
                     /gene="pheS"
                     /locus_tag="VW191_000906"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU9623693.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDVTVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
BASE COUNT         8361 a         6713 c         5695 g         9022 t
ORIGIN      
        1 tcgatttgac tgtcccgatc gatttttcct gttcttattt cactatagtg aattttttca
       61 gatatgattt gacaagaaca ttcttttatt gcagaatcgt gttataaaat ctaacataaa
      121 ggagttctct catggtttta gtagaattta aaaacgtcga aaaatattac ggagactacc
      181 acgcactccg caacatcaat ctccgttttg aaaaaggaca agttgttgtc ctgcttggac
      241 cttctggctc tgggaagtcc actcttatcc gtacgatcaa tggtttagag gctgttgaca
      301 aaggaagtct cctagtcaat gggcaccaag ttgctggtgc cagccagaaa gatttggtac
      361 ctcttcgcaa ggaagtcggc atggtttttc aacattttaa cctttatcca cacaaaacag
      421 tgttagaaaa cgtgacactt gcgcccatta aagttctagg aattgataaa aaagaagctg
      481 aaaaaaccgc caaaaaatat ctggaatttg taaatatgtg ggacaagaaa gattcctatc
      541 ccgccatgct atctggtgga caaaaacagc ggatcgccat cgctcgtggt cttgctatgc
      601 atccggaact cctcctcttt gatgaaccaa catctgctct tgatcctgag actatcggag
      661 atgttctagc agttatgcag aaactggcgc gtgataggat gaacatgatc atcgttaccc
      721 acgaaatggg ctttgctcga gaggttgcgg accgcattat ctttatggcc gacggagaag
      781 ttttagtaga tacgacagat gtcgataact tttttgacaa tccaagcgaa cctcgtgccc
      841 aacaattcct cagcaaaatt atcaaccacg aaagtgacaa agtcaaataa ggaggcgcct
      901 atgaaaaaaa attcttttta tcagcattat tgattagcct tttcggcctt gctgctgcca
      961 aaccagtcca ggctgataca agtatcgcag acattcaaaa aagaggcgaa ctggttgtcg
     1021 gtgtcaaaca agacgttccc aattttggtt acaaagatcc caagaccggt acttattctg
     1081 gtatcgaaac cgacttggcc aagatggtag ctgatgaact caaggtcaag attcgctatg
     1141 tgccggttac agcacaaacc cgcggccccc ttctagacaa tgaacaggtc gatatggata
     1201 tcgcgacctt taccatcacg gacgaacgca aaaaactcta caactttacc agtccctact
     1261 acacagacgc ttctggattt ttggtcaata aatctgccaa aatcaaaaag attgaggacc
     1321 taaacggcaa aaccatcgga gtcgcccaag gttctatcac ccaacgccta attactgaac
     1381 tgggtaaaaa gaaaggtctg aagtttaaat tcgtcgaact tggttcctac ccagaattga
     1441 ttacttccct gcacgctcat cgtatcgatg ccttttccgt tgaccgctct attctatctg
     1501 gctacactaa taaacggaca gcactactag atgatagttt caagccatct gactacggta
     1561 ttgttaccaa gaaatcaaat acagagctca acgactatct tgataacttg gttactaaat
     1621 ggagcaagga tggtagtttg cagaaacttt atgaccgtta caagctcaaa ccatctagtc
     1681 ataccgcaga ttaaggagga taccctatga cagatttatc atcttggaca gcctattttc
     1741 aggattttgg acaatttttc aatggtttcc tctttactct tgccctagcg attggatcct
     1801 ttatcctcgc catggtctta ggaatcttct ttggtgccat gtcaaccagt aaacgtccaa
     1861 ttttacgcat tttagctcgc atctttgtgg aattttacca aaatactcct ctcttggtgc
     1921 agtttgttat tgttttttat ggcctacctc ttatcagtga ccatatcatc atgattccaa
     1981 tttattggac agccgttctc tgcgtgggac tctatcacgg cgcttatatc gctgaggtta
     2041 ttcgttcagg gattcagtct attcctagtg gtcagatgga ggccgccttg tcgcaaggtt
     2101 ttacctatat cagtgccatg cgcttgatta ccttgcctca ggccttccgc atcattctcc
     2161 ctccattgac caaccaaatt gttaacctca tcaagaacac ctctactgta gctatcatct
     2221 ctggagtaga cttgatgttt gtgactaagt cttggtcggc tctcaacgga aactatatcc
     2281 cagccttttt aggtgctact cttctctact ttgccctatg cttccctgtt gcccagtttg
     2341 gtcgcaagat ggagcaagcc aacaaaaaag cctattcact ttaggaggtt actatggaat
     2401 ctattttaga agttttaacc ccagataacc tagtctttat ctttaaagga tttggcttga
     2461 ccctctatat ttctctgatt gccatcatcc tctctactat catcggtacg gtgctagctg
     2521 tcatgagaaa tggcaaaaat cctgtcttgc gaatcatctc cagcatttat atcgaatttg
     2581 tgcgcaacgt tcccaacctt ctctggattt ttactatctt tttggtgttc aaaatgaaat
     2641 ccacaccagc aggtattaca gcctttactc tctttacatc agcagccttg gctgagatta
     2701 ttcgaggcgg tctcaatgcc gtagacaagg gacaatacga agcaggaatg tcacaaggct
     2761 tcacctcagc ccaaatcctc tactacatca ttctcccaca agccatccgc aaaatgctac
     2821 cagccatcat ttctcagttt gttaccgtga ttaaggatac cagcctcctc tactctgtta
     2881 tcgccctaca agaactcttt ggagccagcc aaattctcat gggccgttat ttcgaaccag
     2941 agcaggtctt cagtctttgc atcctgattg ccctcatcta cttcagcttt aacctagcaa
     3001 tttctagcct gtctcatatg ctagccaaac gttggcaaca agctgcagaa taatactctt
     3061 cgaaaatctc ttcaaaccac gttagctcta tctgcaacct caaaacagtg ttttgagcaa
     3121 cttgcggcta gcttcctagt ttgctctttg attttcattg agtataaaca gccactctcc
     3181 agttcaattg gagagttttt tgatgagagt agatatttat agacctcaat ttgacaccat
     3241 acattttcta tgataaaatg taacaaattt atttacaaca aatcgaaatc taaatgaaat
     3301 ggaattttct atgaaatcag tcaaaggact actctttatc atagctagtt ttatcttgac
     3361 tcttttgact tggatgaaca cttctcccca attcatgatt ccaggactag ctttaacaag
     3421 cctatctctg acttttatcc tagccactcg tctcccacta ctagaaagct ggtttcacag
     3481 tttggagaag gtctacaccg tccacaaatt cacagccttt ctctcaatca tcctactaat
     3541 ctttcataac tttagtatgg gcggtttgtg gggctctcgc ttagctgctc aatttggcaa
     3601 tcttgccatc tatatctttg ccagcatcat ccttgtcgcc tatttaggca aatacatcca
     3661 atacgaagct tggcgatgga ttcaccgcct ggtttaccta gcctatattt taggactctt
     3721 tcacatctac atgataatgg gcaatcgtct ccttacattt aatcttctaa gttttcttgt
     3781 tggtagctat gcccttttag gcttactagc tggtttttat atcatttttc tatatcaaaa
     3841 gatttccttc ccctatctag ggaaaattac ccatctcaaa cgcttaaatc acgatactag
     3901 agaaattcaa atccatctta gcagaccttt caactatcaa tcaggacaat ttgcctttct
     3961 aaagattttc caagaaggct ttgaaagtgc tccgcatccc ttttctatct caggaggtca
     4021 tggtcaaact ctttacttta ctgttaaaaa ttcaggcgac cataccaaga atatctatga
     4081 taatcttcaa gccggcagca aagtaaccct agacagagct tacggacaca tgatcataga
     4141 agaaggacga gaaaatcagg tttggattgc tggaggtatt gggatcaccc ccttcatctc
     4201 ttacatccgt gaacatccta ttttagataa acaggttcac ttctactata gcttccgtgg
     4261 agatgaaaat gcagtctacc tagatttact ccgtaactat gctcagaaaa atcctaattt
     4321 tgaactccat ctaatcgaca gtacaaaaga cggctatctt aattttgaac aaaaagaagt
     4381 gcccgaacat gcaaccgtct atatgtgtgg tcctatttct atgatgaagg cacttgccaa
     4441 acagattaag aaacaaaatc caaaaacaga gcttatttac gaaggattca agttcaaata
     4501 agtaatcata ctctcctggg atttcccagg agagtttttt atagccaaat gaaataagac
     4561 aggaacgatg gaattttggc attcaaatca attgataaga atgttttata gtggagtgaa
     4621 ataggatacg aacaaattga ttaggaaaat caaatgaatt tatagaaatc ttttagcagt
     4681 tatgttgtcc tattctagtt tcaaaacgct atagaagcag cattgtgcta gtctagattc
     4741 agtttactat actaaaacga gtagcttgaa atcaaaaaac ccaccctcac aggcaggttt
     4801 tatctgtatt attcagctag attatgcttt accttctgaa ccgaatacgt cgatacgttc
     4861 ttcaaccgat gcttggatag cttttacacc gtcagccaag aatttacgtg ggtcgaagag
     4921 ttttttcttg tcgtattctg cttcgtttgc ttcgtagtca cgagcaaatt tacgagttgc
     4981 gttagcgaat gcgatttggc attctgtgtt aacgttaact ttggcaacac caagtttgat
     5041 agctgcttgg atttgctcat caggaatacc tgatccaccg tgcaatacga ttgggaatcc
     5101 tggaagagct tctgtcaatt tttgcaagtg gtcaaggtct agaccttccc agtttactgg
     5161 gtaagggccg tggatgttac cgataccagc tgccaagaag tcgataccag tttcaaccat
     5221 tgctttagcg tcttcgattg gagccaattc acctttaccg atgattccgt cttcttcacc
     5281 accgatagta ccaacttcag cttctactga gataccttta gcgtgtgctt tttcaacaac
     5341 ttctttagcc aatttaaggt tttcttcaac tggaaggtgt gaaccgtcaa acatgattga
     5401 agtataacca acttcgatac actcaagtgc atcttcgtag tgaccgtggt caaggtggat
     5461 agctactggt acagtgatac ccattgattc aacaaggtta gcgatcaagt tgcgagcaac
     5521 tttgtaacca cccatgtatt tagcagcacc cattgaagtt tggatcaaaa ctggagcttt
     5581 tttagcttct gctgcgcgca agatagcttg agtccactca aggttgtttg tgttaaatcc
     5641 accaactgca taaccgttgt cacgggctgc ttggacaaat ttttctgctg aaacgattgc
     5701 cattttatca ggcctcctgt atatttttat gggtcatccc atttacattg ttcattttat
     5761 cactttttgc caaaaaaatc tagtttttcc cgcagtttcg attgattttc ttctaactcc
     5821 atctatgtaa accctttctc cctagtcttg gacgactttt ggaaaatcta taaagaaggt
     5881 taaacgattc tcctccatct cgaaacgata agctaatttt tcatgttcta atagactctt
     5941 aaccacaaag agccccatac cagacccctt gaccttgcga ctagcattgt cagaaaaaga
     6001 ctgggctagt ttttcttgtt cctctgagct acagctattt tcgataaaaa gttctccttc
     6061 tctttctcca attcgaacta agccacctgg aacagagtgc ttaatggcat tgctgatgag
     6121 attagaaaga atcaacttca taactgatgg gtttagataa gcctgctgat gggtcaaact
     6181 attgtctatc tggagctctc tttcctttgc tagcaaggca taatctttga ccagattttg
     6241 cgtcatctgg aggaggtcaa ttgtttccct atcatctcgc aattcctgca cagaagagag
     6301 ggaaagtatc tgcagaacat ggtgattgag ttcatccaca atccccaagg caactcccag
     6361 atactggtct ctatccttat aacgaccgat attctctctc atattttcga ttaggatttt
     6421 caaactagcc agcggtgttt tcaattcatg agaagctcct cgtaggaatt cgaccttcat
     6481 cttctccagc tggagaatgg cttcattctt ttcatgcaag tccgcaataa cagtcaagag
     6541 atgctggtag aggctattga tttgttcctt gagattacct atctcatcct tagaatccac
     6601 gcgcaatcgc acttgggaat ccaggtccat catccgacgg gtcacccact tgatttccaa
     6661 aatcggtgca acaatagtcc gagcgtagat gtaggccacc aagagggaaa tcagaaagga
     6721 ggccagcaag gtatagggaa gaaactggag actgatttgc tccgcttcct tttgtaaatc
     6781 catggaagct agaaactgga gaatcatagt accaccgtct tgcgttttca cctcgcgctc
     6841 ctcaataaag agagaggttg tctggcggtc tgtgtccaga ggaagactgt ccttgacttc
     6901 taacttgtcc tcggtcatct cacctttgac ggtccccttg atatcactag tctgggaata
     6961 caagtctaac acttgctcga tactctgcct atctttccct tctagggact gggcaatggc
     7021 tgttgccttt tgaccaatgg tttcctgacg atgactcaga taagtcgaag gaaaaagaaa
     7081 ataaatagct aaatgaaggc agataaccag aacactaaat atcgagaagg tatagataaa
     7141 tatctttgca aataaacctg ttcgtttcat tttcgctcca atttataacc aacattgcgc
     7201 acagtgagga tacaatccaa gtctagcttt ttccgcaatt ccttgatata aacatcaata
     7261 acacggtcaa agggaacctc atctgtcgct ttccagacag catcgataat ctgagaccga
     7321 gtcaaggccc ggccttcatt tttcactaga tagtccagaa tttccaactc tttggcattg
     7381 ataggcactt cttgacctgc gaggcttgca ctgtagcttt caaagtccac cttggtatcc
     7441 ttgtaagaaa agattcgtcc tgtatcgtag tagcgcttga aaatcgcgtc caccctcact
     7501 tttaagaggg agagagagaa aggtttttcc agatagccat ctgccaaaga ggcaaaggca
     7561 ctcatcttgt attcctcatc ctgaaaagct gtcaacatca agacaggaac ctgactggtt
     7621 ttacgaatct cagctaggac ttctaagccg ttaagcttgg gcatctggat atccagtaaa
     7681 accagggcca cctcatagct agaaaattgc tccagagctt cctgaccgtc cgctgcctca
     7741 atagtttcat agccacaatc cgtcaaataa tcactgaccc cctcacggat catctcttca
     7801 tcttctacaa ttaaaatttt catactttaa ctgctctcta ttttttattt ttcttagaat
     7861 aaatacctac tctattttct attatagtct cttgctggcc ttttgtctgt aagcaactga
     7921 tcactagata aaacgttgtg aaattccttt ctcataaatt ccataacttt agtatattat
     7981 atttaagcac taaagtacaa agaaagcaac tgaaagcaat gattttcacc actgctttca
     8041 gatttatttt gaattgttaa atagctattc ctatccacta ttcttgaata gaaacacaag
     8101 atgcaatctt tattccagac tcattttttc aaatttattc accatccagc aagagctctt
     8161 ttggttgttt tctaaggaga ttgcttgaag caagcgccat aacgagaacc actagaacca
     8221 aggcaaggac aaaaatgatg ataaagtctg atgtctgaat ggaaatgtct aggctcgaca
     8281 aggtcttgct aaagccatct acttctgcac cgccaccaag gttagaggct tgagccgcct
     8341 tactagcctg tttggcaaca cctgaagtca cattggcaag gacagtgttt ccaattgcac
     8401 gggcagtgta attagctagg aagtaagcag aaactagagc agggatagca atcaagatag
     8461 attcggtgat gaattgaccc aagatacttg cctgcttgag gccgatagag aggagaattc
     8521 ccacttcctt gcgacgggcg ttgatccaaa ggctgagcaa gagggcaagg aggagaactg
     8581 agaagctcaa gctaccccag aagaggaggt tggccatctt gtacatacca gagatagatt
     8641 gctcaagagc tgggtagtta gaggagctct tgacgagtgt gtagctcttc cagttgatac
     8701 cactgatgcc attcaactct ttcataacat catccaagtt cttgtctgct gttacaaaga
     8761 aggttgcgtc cccataaatg gctgtgtctt ctgtgtatcc ataaagtttt gcagcagtgt
     8821 gaatgtctgt aatagctgtg ttttcgtaaa gttcttgtga gtaggttact gctgacttat
     8881 tatgaccatc aaagagtccc ttgattgtca cttcaactgt ttccttggct cctttttcat
     8941 tatctgcatc gtagatatta gagtccagtt taaccttgtc ccctactttc cagccgtgtt
     9001 tggctgccaa atccttgtgc aagaggattt tatccttgtc gtcgttggtt aagtgctctc
     9061 cttcgactag tttataagaa ccagagacaa acttgtcttc tttagaggag tcattgacac
     9121 ctgtaatcat caagctactt ccaaaacgtt tggcacgatc agcagtgaga ttcttcttgg
     9181 tttctggcgt ttcaatcagg tcatatccag tcaaatctcc gatagcgttg atacgtttga
     9241 cataagactc aatggccttg ttttcggtga tttttttgat atcttcaccc ttgatattcc
     9301 cagcaccacg aggcgttcct tggttgacgc gacgattgat ttgcatggag aagctattgg
     9361 tgatattttt aaaggtctcc tgagaagcct tggcagtagc tcccttgatt gacaagccga
     9421 ccaaactcaa gctcgccatg aggagaataa tcaggaagat gacaatcgat ttgaaaaact
     9481 tccttgtaac ataggcaaat gcgttgtgta acatagattc cctttctaga ttttgtttta
     9541 atcattctat caaaataagc tcaaattatt tactagtatt gcgcgtttca gtcagtttct
     9601 tatcctttaa ttcaagtgta atatctgacg cttgtgccac ttctttactg tgagttacga
     9661 caatcacaca tttacctgtt ttctgggcaa gtgatttgag tagttcgaca atatctccag
     9721 cagttttagg atccagattt cctgttggct catcagctag aataactgga gcttctgaga
     9781 ccaaactgcg agcaatggca acacgttgct gttgaccacc tgataactgg agaacattcc
     9841 gcttgatctg gctttcatcc aaaccaagct caagaagtgt attcttgctt gcctttttgt
     9901 tgaccaatcg gatattttcc agcggagaaa gataatctat caagttataa ttttgaaaga
     9961 ccagggaaat atggtgcatg cgatggtaag agtagccctt cttacgaata tcctctcctt
    10021 gaaaaaggat agaaccttca acaggactat ctagaccagc aagtagggac aagagtgtgg
    10081 attttcctgc tcctgactcc ccaataatac tgtaaaattt tccaggttca aaattataat
    10141 cgatctgata taggactgct tcagcagtat tcttataacg gtaggtaaca tcttgtaatt
    10201 gtaataaagt catgatttct ccttcttaac taatagatga taaaatttct ttcggtgatt
    10261 ttctaaataa gaataggaaa caaagggcta cagataagca actaagcaga actagaaaaa
    10321 cataggattc tgcaaaagat aagatgctag ttgataaact gcttgctttg gctagtgtat
    10381 cttgtaagct tgcctgatct ccacttgcta gtagagtttg gagtaggtaa gttgtgattg
    10441 cgtttcctgc aacaaatgct ggaagcaaag ctccaagaga taccaaaact acctctaaac
    10501 agaattgtag gaagatcgag ctcttgcctt ttccaagtgc aagtaaaatc cccacttcat
    10561 agacccgttc tctcaaccag agagacaaaa ccagaattaa ggctccagct cctgctatca
    10621 acatcccata aaggaagatg gtcaggaagg tttggaaagt tgcaactgag tctttgattt
    10681 gttcaaaagc cttgttttcc ttttcgactt ggtagccttg attttccaag gccaagtttt
    10741 ctacctgctt catgagtccg tccatttcct taggattttc tacatagaag cgtgctgcac
    10801 tgacttgagc ttcactattg cccaaaaggg tttggctact ttcatagtct gtaaagactt
    10861 gattttcact gaagtcagaa gacaagcctg tgaatttctc ttgtttttta ccagaaaaga
    10921 tgccgataat ctcaaactct actgtttgtc cttttccaga ttcagactga ccagcatcca
    10981 agccaatctt gtcatgaagc gaaagaccgt tcttcttagc caattcttcg tggataagga
    11041 ttttcttgga atcccctttt tgaaggtgtc gcccttcttt tagattgaaa gccgaactgg
    11101 taaaggttac atccttggat gaatcctcaa gagccgttaa gctaaccaag ttattgtctg
    11161 cagctgataa atcatcacgc tccacgctct gctcgccagt cactgcttcc ttgtctttta
    11221 gttttgcgac cgtctcaagt tcaggagaga cattttccag ccccttaatc ttgcttacag
    11281 atgctaggtc tgacaacttg aatgtctgac cattctctat cttcttaata gaaaaagatg
    11341 tattgagtga tttataaaga ttgctttcta ctgttttgtt ggacttcatc agagtcaaac
    11401 aggctgaaat tccggccaat aagaccaata aaatcagaaa taaaataaaa cttctcagtc
    11461 gctttctgct gacataagcc caagatcttt ggattggatt catttgtcac ctccatattt
    11521 gtaagactat tataaaaccc aaatatgaaa tgtttatgaa atacgaaaaa aaaaatatcg
    11581 agtaggggat aatctctagc ccctctcaca ccaccatacg tgccgttcgg catacgacgg
    11641 ttcaactaac ttttaacgca tgtcgttcaa ggtaataatc caaacacgaa accagtccac
    11701 gtttttcaag gactggtttt gatatagcac gtttaagtac tgacttctga gctactatag
    11761 tagattgaaa ctagaatagt acacctctac ttctaaaata ttgttagaaa tcgatttgac
    11821 ttccctgatc gattcgtcct attcttattt cattttacta taattgatag tggtcgcccc
    11881 agccagatac cttatctgct atccatttag gaacccctaa cttaagcaat ccccataatc
    11941 gtctcgattt cttcttccat tgcttccaga taatcactcg taggcgagta cgcaagcgct
    12001 catctatgct agtgactata cttttcatat ttataattca ttcctttcgt ttcactcaag
    12061 gcacaacaca gaatgaaaaa gtgttgtgat ctttattttg ttttataata atagtgagaa
    12121 agcctatcac tactacaaat cacggggagg tgaataagtg agtggtacag ccactacctc
    12181 gcatattttg tcacatcatt taacggtaca taataagttg taccatctga ataagttgct
    12241 acaatatcat ttgcatgctc tccttcacct ttagcaaagg ttggagctcc tgctggatga
    12301 tttttatttg cctctttcaa tttttcaata atggcatttt tttctgtatc ttttatatta
    12361 tcaggatttt tcactaagat tttgtctgga tatgtcggtt tagcagaaac aatttttact
    12421 gttatttctt ttttattcga agcacttgtc cagtttccag cattatcttt agcatttaat
    12481 tttacagtaa ttcctgaact aggaacttca gtagcaggtt gattatcaac attattcaac
    12541 tttaatttca aaagagctgt tgcatcagac gttttatcaa tcgttatata taatgatgaa
    12601 ttgttattat aaccagtttc ttcatattta gctgtttgtg agctacttga aacagaactg
    12661 aaattatgcc cactacctcc ctgattatct tcaatgctta catctaaatg aacttcccca
    12721 ctattatttg gcttagcaac aactgttata gtaaaataac ataaaatttg cataaataaa
    12781 ttaggtaaat caaagcaact tctaggaatg ttttagcagt cacagtgtac tttcccagca
    12841 tcaagccact ataactctgc acataaaaat ggagaagatg gcaatcctct tctccaaata
    12901 ttaacttctt tacaaaccaa ctatagttga caaagaacct aaaatcaatt gataacacaa
    12961 ggtcaggtcg gtcaactctt tcaactgaag ccctgtcaac tcttcccatt tatcaatctt
    13021 gtattggaga gaattgcggt gcagatagag ttgctgggct gttttagtga gaacagcact
    13081 attttcccaa agagagagaa tgatttcctg aatctgatct tgatccaaaa tcatctggtg
    13141 tagacattcc ttgattggct tcaagtccac gagtctttct cccatactcc aaagatagag
    13201 ctgagaaaaa gtatgaacac cttggtgacc ctgacgccac catgtcttga acaaatcccg
    13261 ctcagctttg attaagtctg atagggcttg atgtcctgtc tgagaccaaa cctgacccaa
    13321 catgatagaa agacgaagtc caaagtcata ctcaactgct tcaatcatat cacttaaaat
    13381 atctcttaca gaagtgtatt tgtcttgttg aagcacgaaa acataatcct gagctccgac
    13441 ctgtagcact gtctgacaat tcggaaaaag agtccgcatc atatctagcc aagaagccag
    13501 attttcctgc tgaaaataag aaagatggca ataaaccaac tgaatctttt taaaaacttg
    13561 cggtgcctgt cccttgcctt caaccagata ggaataccaa gggtttagcg aacgagcctg
    13621 ctcctgctgg gtcaaaaggg caaccaactg cttttcacgc tcgctgagcc cagcttcctc
    13681 cagcaaaatc cactgctgag aagctaaagg gagcgtgaga tagccctctt tctctactgg
    13741 ttggtctgaa atccgagcct caggaaacca gtcttgtagt tcttttgccc tcatgttcta
    13801 gccctccact ttttggatgc accatgaaac caaactctca agacgttcca gattctcagt
    13861 catatggaga tagcccataa ccgcttcaaa tcccgtggac atacgataag tcacgacatc
    13921 tgcattttta gcctttgtgt ggctattggt attgcggcca cgtttgtaga tttcttcttc
    13981 tttttccgtt aggacctgct cctccaacat gagagcaatc aggcgagcct gagccttggc
    14041 tgacacatac ttggttgctt cttgatggag tttattgggt ttggtcatac ctttgaggat
    14101 gaggtgacgg cgaatataca tagaatacac cgcatccccc tcaaaggcta gcgcaatccc
    14161 gttaatgaga ttgacatcaa tcacgtgtcc acctcactcc atccttggta tcaaggagct
    14221 taattccttg agtaaccaat tggtctcgga tttggtctgc tgtcgcaaag tcacgattgg
    14281 cacgcgcctc ttggcgtttt tgaatcaaat cttcaatctc tgcatccaaa acttcctcaa
    14341 caaagacaat tccaaaaatt tctaacatat ctgcaagagc ttgcttgaca cttgcatcat
    14401 agttccctga gttgatccat ttggccattt caaagacaac tgtgataccg ttggcagcat
    14461 taaaatcttc atccatagct gctacaaact tatctttaaa gttttgtaac tcttgggcat
    14521 ccacgtttcc tgtaaatggt tgttcgtaag tattcttcag atacttgaga ttggtctcgg
    14581 catcacgaac tgccttttcc gtgaagttga taggcttacg gtagtgctgg gtcgcaaaga
    14641 agaaacgaag tacttgccca tcaagagttt taagggcatc gtgtaccgta atgaagttac
    14701 ccaaggactt agacattttg acattgtcga tattgacaaa gccattgtgc atccagtagt
    14761 tagcaaaagc cttgcctgtt ttagcttcag attgggcaat ttcattggtg tggtgtggaa
    14821 actctaggtc agctccacca ccgtggatat caatggtatc acctaaaatc tctgtcgaca
    14881 tgactgaaca ctcaatatgc caacccggac gtccaggtcc ccaaggacta tcccaagaaa
    14941 tctcacctgg tttggaagat ttccatagag caaaatctac aggattttcc ttacgagccg
    15001 tttcttcatc ggtacgacct gaagcaccta gatccaaatc ttccaaggtt ttattagcca
    15061 atttagcata gttgtgggat ttttctacac ggaaatagac atccccttga ctctcatagg
    15121 caaagccttt ctcgatcaag tcttccacaa aacggatgat gtctgccata aactccacta
    15181 cacgcggatg gcgagtcgca ggtttcacgc ctaaggccgt cacatcctca cgaaaggcag
    15241 cgatgtactt atccgcaacc tcctgaggcg tgatgccttc ttccctggca cggttgataa
    15301 tcttatcatc cacatctgta aaattggaaa tataggcaac cttataccca cggtactcaa
    15361 aatagcgacg aatcgtatca aaagctaccg tcgaacgggc gtttcccacg tggatatagt
    15421 tgtacaccgt tggcccacaa acatacatct tgatcttgcc gtcctcaatc gggacaaatt
    15481 ctcgcaaatc acgagacatg gtgtcataga ttttaatcat aaatcataat caggaaagct
    15541 gaaatccaag aacaattagt ttcatcacta aaagttcaag taaatttcag tccgaatatc
    15601 tctacacttc ggaatccctt gctcctttct cattcagata aaccacctga gtctgtttga
    15661 caaagccaat tttttcatac aaacgtttgg cacctacatt gctatcttcc actgcaatct
    15721 gaaattcctt gtcattttgc tcaattagtt ggttgacgag ggattttgct aagtagcttc
    15781 catagccttt cccacgttca ggttccgata ttgctaaacc gtagaagtaa ttcgtattag
    15841 tcgataaatc taccgtgcaa gtcccaataa cctgaccgtc ttttaacaaa atatatagtc
    15901 ggctttctgg atccttcaga gcttcagcga catatttatc cacaacttct ctcgattcat
    15961 gttcctctga aaatgcctga aattttaatt gactaatttg atcctgatac gaactatctg
    16021 ctaacaaaac ttcaatatgg gaaacatttg ctaacggata aggtcttcta tccttaccta
    16081 accaagtttc tgtctcttca tcctcgatta gtccccagtt actggcaaag tcaggatgat
    16141 tctctaaaaa aatacgttct gtctgaaaag tgactgatcg aatggggaaa gaagctgttt
    16201 ctctctcaaa actagtaaac aatacacgcg caatcccctg acggcgatga cctggatgaa
    16261 ccagtatcgt cacttctaca tcttggtcat ctgcatagac agttaataaa ccaacaagtt
    16321 cgcctttttc ataataaagg aaaaaggcgg gcatgtttgg gtcaaaatta agcatgttag
    16381 agagataggg atcgcgatag gtaccgtcat agttttggca acagttaatt acttttttcg
    16441 cctcagatag ctcctcttgg cttaacttgt ttcttgcttg aatcatatag gtatcctcta
    16501 caaaccagac gatctgtgac tggcatcttt agcctgctcg agtttattga cataatactc
    16561 tcgtttttct tcgacttcgt gaatgacagg ctcatctttc ttaccatgaa gacggacaat
    16621 cttggccgga ataccgacaa ccgtcacatc actaggtaca tctgctacga caactgctgc
    16681 agcaccgacc ttggcatttt caccaatttc cacaggtccg ataacttggg catgggctga
    16741 tatgagggct ccctttcgta cagtcggatg gcgtttgcca cagtctttcc ctgttccccc
    16801 aagagtcact ccgtgataga gaagaacgcc tttttcaaca atcgctgtct ctccaatcac
    16861 cagaccagaa ccatggtcaa taaagacacc tgaatcaatc tgggctcctg gatgaatctc
    16921 aatctgagtc caaaagcgcc aaaactgact gtgcatacga gctaagagtt tgaagccgtg
    16981 cttccagaga aaatgcgaga gacggtgggc cgccaaggcc ttgacacctg gataagtcag
    17041 caaaacctcc aaagtggtgc gggccgctgg atcattttct tttacgatat caatggtttc
    17101 gcgccaccac cccatacatt tctccttttc ttattctgaa tcttttggtg tttctgtaaa
    17161 ttctttctta ggtttgtgat ccttgtgatg acgtgggcgg tgaggacgct cagacttttc
    17221 acctttttca tcatgctcag gttttggcgg acgaggtaga agagccttca tagaggcatc
    17281 gatacggcct ttttcatcaa tcttgataac cttaacatca acttcatccc cgatttctac
    17341 caaatcctct acacgattgg tacgagtcca agccatctca gagatatgaa caagggcatc
    17401 tgtcttatca aagaggttaa caaaggcacc aaatttctcg atacgaacga ctttagcacg
    17461 gtaaacttca tccactttgg cttcacgaac caaaccagca ataatttctt tggcacggtt
    17521 aatagcatct tggtcactag agtagataga cacatttcct tcttcgtcta tatcaatctt
    17581 aacacctgtt tcagcgataa tcttgtcgat ggtttctcca cccttaccga tgacaatctt
    17641 aatcttgtcc acatcaatct tgatcgtatc aattttcgga gcagttggag ccaattctag
    17701 acgaacttct ggaatcgttg cttcaatgac atcaaggatt tcaaaacgcg ctttcttggc
    17761 ttgagcaaga gcctccgtca agatttctgc agtaatccct tgaatcttga tatccatttg
    17821 aagggctgta atcccatcac gagtacctgc aaccttgaag tccatatctc caaagtgatc
    17881 ttccaaacct tggatatctg tcaatactgt gtagttattt ccatctgaga taagtcccat
    17941 agcaatacca gctactggcg ccttgattgg cacaccacca gccataaggg caagagttcc
    18001 cgcacagata gaagcttgag atgaagaacc gtttgattcc aaaacttctg ctactagacg
    18061 gatagcgtat gggaattctt ccaagcttgg caagacttga gcaagagcac gctcaccaag
    18121 ggcaccgtga ccgatttcac gacgacctgg cgcaccgtaa cgacctgttt cccctacaga
    18181 atattgaggg aagttatagt ggtgcataaa gcgtttcttg tactctggat ccaaaccatc
    18241 aatgatttga gtttctccca tcggagccaa ggtcaagact gaaagagctt gagtttgccc
    18301 acgagtaaag agacctgaac catgtacacg aggaaggaag tcaacaaccg catccaaagg
    18361 acggatttca tcgaccttac gaccatcagg acgcaccttg tcttctgtaa ttaaacgtcg
    18421 cacttctgcg tgttccattt gttccaagat ttcagccaca tcacgcataa tacggtcaaa
    18481 ttcttcgtgg ttcgcatatt tttcttcgta aacggcagtc acttggtctt tcactgcttg
    18541 agttgcagct tcacgggcca atttctcttc tacttgaact gccttttgga ggtcactgtt
    18601 gtaggctgca atgatttcag cttgcaattc agcatccacg tgaagcagtt ccacttctgc
    18661 tttttcttta ccgacagcag caacgatttc ttcttggaag gcaatcaatt ctttgacagc
    18721 ttcgtgccct ttaagaagcg cttccaacat gatttcttct gacaattctt tggcaccaga
    18781 ctctaccatg ttgatagcgt gcttggttcc agctactgtc aattcaagaa gagattgctc
    18841 tgcttgttct tgacttgggt tgatgatgat ttggccatct acatatccca cttgtacccc
    18901 agcaattggt ccgtcaaatg gaatatctga aatagacagt gccaaagatg aaccaaacat
    18961 agcagccatt ggtgcagatg cattttcatc ataagaaagc actgtattga tgacttggac
    19021 ttcattacgg aaaccttccg caaacatagg acgaatcgga cggtcaatca aacgcgctgt
    19081 caaggtcgca tctgttgaag gacgtccttc acgtttcata aagccaccag gaaacttccc
    19141 agccgcatac attttttctt cgtagttgac ttggagtggg aaaaaatccc cagttgccat
    19201 tttcttagac ataacggcag cagtcaagac agttgactca ccgtaacgta cgacaacaga
    19261 gccatttgct tgcttagcaa cctgaccagt ctctacaatt aactcacgac ccgcaaaagt
    19321 cgtttgaaac acttgttttg ccattttaat cccctttgga ttgatgaaat tatacgcctt
    19381 gcctacaaag atcaagatac caaggacgtc aaaagcaaag taaaaatagg aaactgacga
    19441 agtcttcgat gaagacaaga cagtttatct tttttacaca gcttttcggc cgtgttcaat
    19501 tactcaagat gctaagccgt caagcaactc aaaggaaaat aggaaatcga acgacggagc
    19561 gactgctcct aggcagattt atctttttcc acagagttgt aggcgtgttc agttttcaag
    19621 atacatcatt agaaaggttt aatattaaag tatctaaacc tttcacgcta atcgctatcg
    19681 ggcgattagc taaatgcttt actaactctt tcgtcaaata acatcgattt gactcactcg
    19741 tgtcgttaaa tcttacagtt taaatgcatt gtattattta ataccttcat ctttgtatca
    19801 agtacgtaca gaatctattt tatcatattt ttcttaaaaa gtgaggtctt taccattaaa
    19861 aaggaaccat tcccctcacc tgagaagaat ggtttgcttt tattatccta gagactggtg
    19921 attaaacaag gcatgggttg cttgatggat gtattttgct gtatcagcat tattcatcgt
    19981 atagagatgc acaccggcaa catcctgagt taccaagtcc acgatttggt ccactgcata
    20041 ggcaagtcct gctgctctga gcgactcagg gtcatgctca tacttgtcta agatggcttt
    20101 aaatttgcgt ggaagatgga tattctcaca agtcttcaag agtcggagag cctgatttcg
    20161 attcagaatt ggcataattc ctgcatgaat gggaacatca atcccagcca agatgcactt
    20221 gtcctgaaaa tcatagaagc gctcattgtc aaagaagagc tgagttacga ggctcgaaca
    20281 gcctgcatcc actttcttct taagattttg aatatctgaa atctgatttg gcgaatctgg
    20341 atgcccttct ggatagcaag ctccaataat atcaaagtga ggggcttgtt ccttgataaa
    20401 ctcaatcaag tcggttgcat agcggaaatc cttttgtggt tctacgtctg gaataatatc
    20461 cccacgaaga gccaagattt tctgaacccc aactttgtcc aagtcagcaa tagtttcagc
    20521 aaccttgtcc ttagttagat aaatagctgg caagtgggca atggtcggaa tcgccaagtc
    20581 attttggata aagtcagcca aacgaaccgt cgtttccttg atattaaatt tattattgct
    20641 ggcagttaca ctgataaaat ggggagccaa ctcctgcata tcctgcaagg ctgaaataat
    20701 gttaccatta cccacggctg ggtttggagg gaacacttca aatgagagtg acggtgtttg
    20761 gcgtgacata tgtaataacc ttttctagtt gatttctttt tgaacaacca ctgtatggag
    20821 agaaatccaa tcttacaatt tctcacgcgc agctttagct gcttcaacaa ggcggatcaa
    20881 gctttctttt gtttctggga taccacgtgt tttcaaacca cagtcagggt tgatccaaac
    20941 tttcttgctt ggcactttag caaggatggc ttcgattgtg ttgtcgattt cgccttcatt
    21001 tggtacacga ggtgagtgga tatcgtaaac cccaggtccc acttctgttt ggaagttttt
    21061 cgctttgagt tcgtccaaga tttcaaggtt tgaacggctg gcttcaaagg aaataacgtc
    21121 tgcatccatg ttatcgatag ctgggatgat atctgtaaat tctgagtaac acatgtgagt
    21181 gtggatttgt gtgtctgacg ctactgttga gtgtaccaag cggaaggcag gaattgccca
    21241 gtcaaggtaa tcttcgtacc agtcactacg acggagtggc aatttttcac gaagagcagc
    21301 ctcatcaatt tggatgattt tcacaccagc agcttcaagg tcaagcactt catccttgat
    21361 agcaagggcg atttgaagag tagaatcctt gatagagatg tcttcacgtg ggaatgacca
    21421 gttgaggatg gtaacaggtc cagtcaacat acctttaaca ggtttgtttg tacggctttg
    21481 tgcatagcta gaccatttaa cagtgatagg gttaagacga gtgacatcac cccagatgat
    21541 tggtggtttc accccacgca taccgtatga ctgtacccaa ccatttttag agaagaggta
    21601 acctgacaag ttttgaccga agtactcaac catgtcatta cgctcaaatt caccgtgaac
    21661 aaggacatca aagtcaatat cttcttgcca cttgatccat tcgtcaatcg tttcagcaag
    21721 gaaagcgtcg tactcttttt gagacaattc acctttacgg taagccaaac gtttggcacg
    21781 aacttctttt gtttgaggga atgaaccaat cgttgttgtt ggaagagctg gaagtttgaa
    21841 agcttcttct tggatagctt cacgttctgc aaaggctggc aaacgagtgt aatctgcgtc
    21901 tgtcaagcca gcgatacgcg cacgaagttc agcattttca ccaacacgct cagtcgcaaa
    21961 gagttctttg ttggctgcaa gagcttctga accttgacca ttgcggatag catccaagtc
    22021 acggatttca tccaattttt caactgcaaa ggcaaagtgg ttcaagagtg ctggttcaaa
    22081 ttcttcatta gcagttgtaa atggcacatg aagaagtgag caagagcttg tcaaaacgat
    22141 gttttcagct gggatttgct caagaacagc caagctcttt tcgtagttgt tgcgccagat
    22201 gtttttacca ttgacaatac ctacatagag agtcttgtca gctgggaagc cacctttaac
    22261 gagttcaaga gttttcttac cttcaacaaa gtcaagaccg atagcatcta ctggcaagtt
    22321 tacaaggtca gcgtatacgt cacgaacatc accgaaataa gtttgaagca agacttcaag
    22381 accttttttg tcagccaaga gtttgttgta aaggttcaag aagagagctt tttcttcagc
    22441 tgtcaagtct tttacaagag ccgcttcatc caattggatg cgagtcgcac caagttcagc
    22501 caatttagca aaaacttctt ggtaagcaac tactaagcta tctacgaagt cgtctgcttt
    22561 cacgccttct tcaaagtctg acaattgaag gaaagtgaat ggacctacaa gaacaggacg
    22621 agtgttcaat ccaagttctt ttgcttcttg gaactcatcg aaaatcttgt gaccagccaa
    22681 ttttacttga gtgtcttttt caaatttagg aacgatgtag tggtagttag tgttgaacca
    22741 tttcttcatt ggaagggcgc gaacgtcccc tttttctccc tggtaaccac gtcccaaagc
    22801 gaagtagcgc tcaaggtcag acaagtccaa gttttgaacg gatgcaggca ccacgttgaa
    22861 aaggaaagcc gcatctagga agttatcata gtgagaaaag tcatttgatg gaatttcagt
    22921 gatgcctttt tctttgacaa tgttccagtg tttagcacgc aagtcttttg ctgctgataa
    22981 aagttcttcc tctgagattt cttttctaaa gtatttttca gttgtaaatt ttaattcacg
    23041 gaattcgccc aaacgaggga aaccgacgat tgtagttgac atgatgtgtc ctccaaaatt
    23101 tgttgttgaa actatcttaa cagaaaagaa agcgtctgta taattgtaaa aaattagggt
    23161 ttgatatagt ttgaaactat atatctgttt cggacaaaag aaaaagactt gaagcaaacg
    23221 tctcaaatcc tttgtatagc ttgttttata gctgtatttt agttagaatg ctcaaacgcg
    23281 ttattagtaa ttcttataag tgactatggc ttgttattag aaaagactat aactgattct
    23341 agtcaacttt ttccctgttc aagtgggacg attgctagtg tctttcctaa actggctagg
    23401 acttttaaga ctgtatccaa ctgaggacta gtctttcctg tctccatcct agctatgaca
    23461 ggctggctta ttccactgac ttcttccagc tttttctgac tgattccttg ttcataccta
    23521 gcctcaatca actcgctcat gatagccact cgcatatcac tttcaaggat ttcctccttg
    23581 ctaaagagct cagatggaca tccttccaat tactcccaat agcactattc ttcatcactt
    23641 aaccctcttt tttttacgtc tatgtatttt taaaaaaatg agcgaattat gattcgatag
    23701 attgatcagc gggtttaaaa ttgggactaa cttgtttctt aacagatttt ccttttctag
    23761 gataaagcaa atcctgtttg cttaacccca actttccatg atgaatccaa tagtaaatgg
    23821 ttgaaattcc cacgttaacc cctttagcca tcaccatcat ttcaggcgaa aatttttggt
    23881 tatgatagtg gagaatcttt tcctttagtt tcttagtcaa gcttgatttc ttgaccgagt
    23941 gcttgcgatt attttcataa gactgttgag cgtagtcggc agaataaatc tctttgaagc
    24001 gcccttttcc aagacattgt cggactgtcc catacttgat ttcagtatgg atagtttgag
    24061 gagcttttcc aagtagagag gcaatttctc tatttgattt tccttctttt ttccatcgtt
    24121 cgattaagcg acggctatcg attgtcaaat gtttgccttt tgtagtataa ttgtcttgca
    24181 tttctgtgcc ttttaatcat ttcaatctta aattggactt tttttacttg ggttgtactt
    24241 aatctatgag gaagacaaga aaaagaatat caatcaagta aagtcacaaa gtcacattag
    24301 ctccgagcaa ccattgcaaa ttgaggtact cacacaatga ttaaaacatt tctctctgcc
    24361 ctttcggtca ttctctttgt tatccctatc atagcttatt ctttttttcc atcttctaat
    24421 cttaacattt ggctatctac ccaacctatc ttggcacaga tttatgcctt ccccttagct
    24481 actgcaacta tggctgctat tttaagtttc ttattttttt tcctatcttt ttacaagaaa
    24541 aataaacaaa tacggtttta ctctgacatt ttgctcttac tatcgctcat attactatta
    24601 tttggaacag ataaaaccct ttcttctgca tcaaataaga ctaaaacctt aaaattagta
    24661 acttggaacg tcgctaatca aatagaagca caacatattg agcgaatttt tagccatttt
    24721 gacgccgata tggctatatt ccctgaacta gctaccaata tcagaggtga gcaagaaaac
    24781 cagagaatca aactattgtt tcatcaagtt ggactttcta tggccaacta tgatattttc
    24841 acttctccac ctaccaatag tggaatagct cctgtgactg tgattgtcaa gaaaagttat
    24901 ggtttctata cagaagctaa aacttttcat acaacacggt tcgggacaat tgtattacat
    24961 tcgagaaaac aaaatatacc agatatcatt gccttgcata ctgctcctcc tctgccaggt
    25021 ttaatggaaa tctggaagca agacttaaac atcattcata atcaattggc ttcaaaatat
    25081 ccaaaggcta ttattgcagg tgattttaat gcaactatgc gtcatggagc acttgcaaaa
    25141 ataagctctc atagggacgc attaaatgta ctgccacctt ttgaaagagg aacttggaat
    25201 agccaaagtc caaaactttt taatgcaaca atagatcata ttttattgcc taaaaaccac
    25261 tactatgtta aagatttaga cattgtaagt tttcaaaact ctgatcatag atgtattttt
    25321 acagaaatca cattttaatt attttatata aaatcacccc tctagtgttc ataaactaga
    25381 gggggaattt gtatcctact atcgtttaac gcacttctgc attgactttt tcttcgagag
    25441 acgcttggat tttttccatg tagcgtgcga cttcttcgtc cgttaagctg tcttctggat
    25501 tttggaaggt caagctataa gccattgact tcataccaag tcccagtttt tcacctgaga
    25561 agacgtcaaa gagtttgata tctgtcaaac gtttcacgcc ggcagcttgg atagcatcta
    25621 caacttcttg gtgagtcact tctgccttga ggagaagggc aacgtcacgg ctgactgctg
    25681 ggaatttggt gatttccaca aatggaacag caggttggag cgccccttcg atggctgaaa
    25741 ggttaagctc agctacatac gtttctggaa tatcgtaagc cttggcagtg actggatgca
    25801 cttggccaag gaaaccaaga acttggtcac cgagtgaaat cacggctgta cgacctggat
    25861 gaaggctagc gatttcagat gttgctgtat aggttacttg gagtcccaaa cgagtaaata
    25921 gggcttcaag gattccctta gcatagaaga aatcaactgg aactgctgct gtttggaaat
    25981 ctttttcagc aaccaagcct gtcaaggcaa aggcaaagct gttgatctca tttggaagtt
    26041 cttcttttgg attacctgtt tgttcaaaga cttttccaat ctcataaagg gccaagtttt
    26101 tattcttacg tgccacgttg taggcaacgg tatcaaggat ccctgaaatc atattttgac
    26161 ggaggactga acgatccaca gtcattggcc acatgagttc agtaaggtta cttggttgag
    26221 ctgtgaactc aactgctttt tcaggagttg tcagagcata ggtgatgatt tctgtcaaac
    26281 ctgctccttc agcaatggta cgaacttgac ggcggagttt ttgtatcaca gtcaattcac
    26341 cagctgtacc atcgtctttt ggaaggctgg ttggcaagcg gtcatatcca tagatacgag
    26401 cgatttcttc aaagagatca gcttcgattg tgatatccca acgacgacgt ggtacgctga
    26461 ctgtaaagct gtctgcattt ccagaaagac caaagccaag acgacggaag acgtcttcga
    26521 catcagcata agacagctca gttccgagga cacggttaac atcagcaaga gttgaagaaa
    26581 cttctacatc tgaagtatca agctcacccg ctgaaacgat acccttacgc accgtcgcac
    26641 ctgcaagttc cgcaatcagg ctagctgccg catcaagggc ttcattaact gttgccacat
    26701 taattccttt ttcaaagcga gaagatgact cagaacgaag gttcaggtga ccacttgtct
    26761 tacggataga tttgccattg aaaacagcag cttcaaggac aacacgacta gatttttcag
    26821 agatttctgt tgcttgacca cccatgacac ctgcaagggc tactggcttg tctgcaacag
    26881 tgatgactag gtcattcacg tccaagtcac gttcttcacc gtccaaggtc accaattttt
    26941 caccagcacg cgcttcacgc acacggatgt cagtcccttc aaatgtgtcc aagtcaaaag
    27001 catgcatagg ttgaccaaag tagagcagga tgtagtttgt cacgtctaca acgttattga
    27061 tgggacggat gccttcgttc atgagaaggt tttgcaacca ttgtggactt ggtgcgatag
    27121 tcacattgtc caagatacga gctgcatagt aaggcgcctt gtctgtccca atgctgacag
    27181 aaagggcatc tgccgcagct tcattagttt ctgttagagt aaattcttta aagttgactg
    27241 ccttgtcata gatggctgcc acttcgtgag ccactccaca catagaaagg gcatctgcac
    27301 ggtttggtgt gatggaaagt tcgatgattt catcatccaa gtctaggtaa gaaaagactt
    27361 cctcacctgg cacggcattt tctggcaaga tttggatgcc atctgcgaat tccttaggca
    27421 caactgagtc agaaattccc aattcaccaa gtgaacagat cattccaagt gactccaaac
    27481 cacggatttt tccttttttg attttgtagt tatcagcgat acgagctcct ggaagagcca
    27541 ccatgacctt gatcccagca cgcacatttg gggcaccaca aacgatctga cgctcttctt
    27601 cgccaacgtt aacctgacaa acatggaggt gagtctctgg cacatcttcg caagacaaga
    27661 cctcaccgac gacaattttt gagagaccag cagctggtga tccgacaccc tctacctcga
    27721 ttcctgtagt tgacattttt tcagccaact cttgtgatgg cacatcaatg tccaccaatt
    27781 cttttaacca tttataagat acaagcataa tttagttctc cagaatgacc attgccacac
    27841 tggttctttt cctttcctat catttcaata gaagaatcct cttcttacct taatttcttt
    27901 ctcagtaacc aatccgtatc tactttttga ccaaccataa aatgatgttg gctaaatttt
    27961 tcaaaaccat atcggttata aaaggcttga gcttttgtat tatgctccca aacacctagc
    28021 caagcccaag aaaaactatt ttttgtagca agttcaagtg cgaattcaaa cagttgctta
    28081 cctagtccaa atccttggaa tttttgtagc acatagaggc gttgaatttc aaaagcgtcc
    28141 tctaattctc tctcagtttg agcacttccc cagttgactt tgagaaaacc agctatctcc
    28201 tcctcatgca taatgaaata ggtttcagag tcaggatttc ccaactcagt tgacaaagtt
    28261 tttagactat aagcctcttc aaagtattcc tgtaactgct cttccgtatt atcatacgca
    28321 aaggtttcac gaaaggtttg tttggcaatt ttagccaaca cctcaacatc tgccatttct
    28381 acttttctaa tcattattta aactgttctg agaagcggac atctccttgg tagaatccac
    28441 ggatatcgtt gattccataa cggagcatag ctacacgctc ttgtccaaga ccaaaggcaa
    28501 agccagagta tacagtcaca tcgataccac tcatttcaag gacacgtggg tgaaccatac
    28561 cggcccccat aatttcgatc caacctgttt tcttacatac attacagcct tctccaccac
    28621 acttgaagca agaaacatcc acctcaacag atggctctgt gaatgggaag taagatggac
    28681 gcaaacgaat ttgacgctct tcaccaaaca ttttttggac aatcaactga agcgttcctt
    28741 gaagatcagc catagagata tttttcccaa ctaccaagcc ttcgatttgg tggaattggt
    28801 gactgtgggt cgcatcgtcc gtatcgcgac ggaagacacg ccctggcgag atcatcttca
    28861 aaggaccttt agaaaaatca tgggcatcca tagcacgcgc ctgaactgga gacgtgtggg
    28921 tacggagcaa gatttcttca gtgatataga aagtatcctg catatcacga gctgggtggt
    28981 cttttggaag gttcatacgt tcaaagttat agtagtcttg ctccacttca aaaccatcca
    29041 cgacttgata acccataccg atgaagatat cttcgatttc ttcactggtt tgtgtcaaaa
    29101 cgtgacggtg accagtcgca actggacgac ctggaagcgt cacatcgata ctctcgctag
    29161 ccagttgagc cgcgactttc ttttcttcca agagcttagc tgtttcttca aaagcagctg
    29221 tcaagacatc acgagcttca ttgacgtgtt tcccgatgat tggacgcatc tcagcagaaa
    29281 catctttcat ccctttgaga atttcagtga gcgaaccctt tttaccaagg acagagacac
    29341 gcaaatcttg catctctttt tcatttccag cagtaatctg cttcaagcta gccagcgttt
    29401 cttcgcgaag cgcttttaat tgttcttcaa tagttgacat atttcctcca tcagtctctc
    29461 gtagataaaa agaaaaccac atgccaaaaa ctccactcgg agcgttgaca cgcggtacca
    29521 tccgttttca tctgacaagt cagaccttca tttctaaatc catgcgcaag tgaattcacc
    29581 cagctttcat atagagagct tgcagtcacg gctctcctcc ctgatatact tcccttgggc
    29641 tactagtctt tcagatttct attcaattac cacttagttt atcagatttt taccattctt
    29701 gcaagaccta tcttacttct gcttgttagc ttattcttat ctaactttat agcggattga
    29761 aataagatga aaacaaatcg attgggaaag t
//
LOCUS       DAVKJU010000022        29052 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVKJU010000022 DAVKJU010000000
VERSION     DAVKJU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29052
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.022"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(52..1389)
                     /locus_tag="VW191_000907"
     CDS             complement(52..1389)
                     /locus_tag="VW191_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEU9623694.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(1399..1665)
                     /locus_tag="VW191_000908"
     CDS             complement(1399..1665)
                     /locus_tag="VW191_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU9623695.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(1930..2316)
                     /gene="rplQ"
                     /locus_tag="VW191_000909"
     CDS             complement(1930..2316)
                     /gene="rplQ"
                     /locus_tag="VW191_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU9623696.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(2328..3263)
                     /locus_tag="VW191_000910"
     CDS             complement(2328..3263)
                     /locus_tag="VW191_000910"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU9623697.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(3306..3689)
                     /gene="rpsK"
                     /locus_tag="VW191_000911"
     CDS             complement(3306..3689)
                     /gene="rpsK"
                     /locus_tag="VW191_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU9623698.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(3707..4072)
                     /gene="rpsM"
                     /locus_tag="VW191_000912"
     CDS             complement(3707..4072)
                     /gene="rpsM"
                     /locus_tag="VW191_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU9623699.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(4090..4206)
                     /gene="rpmJ"
                     /locus_tag="VW191_000913"
     CDS             complement(4090..4206)
                     /gene="rpmJ"
                     /locus_tag="VW191_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU9623700.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(4231..4449)
                     /gene="infA"
                     /locus_tag="VW191_000914"
     CDS             complement(4231..4449)
                     /gene="infA"
                     /locus_tag="VW191_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU9623701.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(4566..5204)
                     /locus_tag="VW191_000915"
     CDS             complement(4566..5204)
                     /locus_tag="VW191_000915"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU9623702.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(5355..6665)
                     /gene="secY"
                     /locus_tag="VW191_000916"
     CDS             complement(5355..6665)
                     /gene="secY"
                     /locus_tag="VW191_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU9623703.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(6678..7118)
                     /gene="rplO"
                     /locus_tag="VW191_000917"
     CDS             complement(6678..7118)
                     /gene="rplO"
                     /locus_tag="VW191_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU9623704.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(7263..7487)
                     /gene="rpmD"
                     /locus_tag="VW191_000918"
     CDS             complement(7263..7487)
                     /gene="rpmD"
                     /locus_tag="VW191_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU9623705.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(7459..7953)
                     /gene="rpsE"
                     /locus_tag="VW191_000919"
     CDS             complement(7459..7953)
                     /gene="rpsE"
                     /locus_tag="VW191_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU9623706.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(7971..8327)
                     /gene="rplR"
                     /locus_tag="VW191_000920"
     CDS             complement(7971..8327)
                     /gene="rplR"
                     /locus_tag="VW191_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU9623707.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(8411..8947)
                     /gene="rplF"
                     /locus_tag="VW191_000921"
     CDS             complement(8411..8947)
                     /gene="rplF"
                     /locus_tag="VW191_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU9623708.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(9139..9537)
                     /gene="rpsH"
                     /locus_tag="VW191_000922"
     CDS             complement(9139..9537)
                     /gene="rpsH"
                     /locus_tag="VW191_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU9623709.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(9822..10091)
                     /gene="rpsN"
                     /locus_tag="VW191_000923"
     CDS             complement(9822..10091)
                     /gene="rpsN"
                     /locus_tag="VW191_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEU9623710.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(10109..10651)
                     /gene="rplE"
                     /locus_tag="VW191_000924"
     CDS             complement(10109..10651)
                     /gene="rplE"
                     /locus_tag="VW191_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU9623711.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGSKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(10675..10980)
                     /gene="rplX"
                     /locus_tag="VW191_000925"
     CDS             complement(10675..10980)
                     /gene="rplX"
                     /locus_tag="VW191_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU9623712.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(11058..11426)
                     /gene="rplN"
                     /locus_tag="VW191_000926"
     CDS             complement(11058..11426)
                     /gene="rplN"
                     /locus_tag="VW191_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU9623713.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(11452..11712)
                     /gene="rpsQ"
                     /locus_tag="VW191_000927"
     CDS             complement(11452..11712)
                     /gene="rpsQ"
                     /locus_tag="VW191_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU9623714.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(11737..11943)
                     /locus_tag="VW191_000928"
     CDS             complement(11737..11943)
                     /locus_tag="VW191_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU9623715.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(11953..12366)
                     /gene="rplP"
                     /locus_tag="VW191_000929"
     CDS             complement(11953..12366)
                     /gene="rplP"
                     /locus_tag="VW191_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU9623716.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(12370..13023)
                     /gene="rpsC"
                     /locus_tag="VW191_000930"
     CDS             complement(12370..13023)
                     /gene="rpsC"
                     /locus_tag="VW191_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU9623717.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(13036..13380)
                     /gene="rplV"
                     /locus_tag="VW191_000931"
     CDS             complement(13036..13380)
                     /gene="rplV"
                     /locus_tag="VW191_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEU9623718.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(13392..13673)
                     /gene="rpsS"
                     /locus_tag="VW191_000932"
     CDS             complement(13392..13673)
                     /gene="rpsS"
                     /locus_tag="VW191_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU9623719.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(13777..14610)
                     /gene="rplB"
                     /locus_tag="VW191_000933"
     CDS             complement(13777..14610)
                     /gene="rplB"
                     /locus_tag="VW191_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU9623720.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(14628..14924)
                     /locus_tag="VW191_000934"
     CDS             complement(14628..14924)
                     /locus_tag="VW191_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU9623721.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(14924..15547)
                     /gene="rplD"
                     /locus_tag="VW191_000935"
     CDS             complement(14924..15547)
                     /gene="rplD"
                     /locus_tag="VW191_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU9623722.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(15572..16198)
                     /gene="rplC"
                     /locus_tag="VW191_000936"
     CDS             complement(15572..16198)
                     /gene="rplC"
                     /locus_tag="VW191_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU9623723.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(16415..16723)
                     /gene="rpsJ"
                     /locus_tag="VW191_000937"
     CDS             complement(16415..16723)
                     /gene="rpsJ"
                     /locus_tag="VW191_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU9623724.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(16990..17607)
                     /locus_tag="VW191_000938"
     CDS             complement(16990..17607)
                     /locus_tag="VW191_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEU9623725.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(17604..18194)
                     /gene="nrdG"
                     /locus_tag="VW191_000939"
     CDS             complement(17604..18194)
                     /gene="nrdG"
                     /locus_tag="VW191_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEU9623726.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(18187..18693)
                     /locus_tag="VW191_000940"
     CDS             complement(18187..18693)
                     /locus_tag="VW191_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623727.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(18737..18877)
                     /locus_tag="VW191_000941"
     CDS             complement(18737..18877)
                     /locus_tag="VW191_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623728.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(18889..21096)
                     /gene="nrdD"
                     /locus_tag="VW191_000942"
     CDS             complement(18889..21096)
                     /gene="nrdD"
                     /locus_tag="VW191_000942"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEU9623729.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHTAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKQEDYAWK
                     KAQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(21216..22754)
                     /locus_tag="VW191_000943"
     CDS             complement(21216..22754)
                     /locus_tag="VW191_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9623730.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(22791..22934)
                     /locus_tag="VW191_000944"
     CDS             complement(22791..22934)
                     /locus_tag="VW191_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623731.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(23012..24544)
                     /gene="cls"
                     /locus_tag="VW191_000945"
     CDS             complement(23012..24544)
                     /gene="cls"
                     /locus_tag="VW191_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEU9623732.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(24690..25232)
                     /locus_tag="VW191_000946"
     CDS             complement(24690..25232)
                     /locus_tag="VW191_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEU9623733.1"
                     /translation="MTPFFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDIDG
                     TYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVNG
                     NQIVVDDMDRDPSDQIVLTK"
     gene            complement(25229..26479)
                     /locus_tag="VW191_000947"
     CDS             complement(25229..26479)
                     /locus_tag="VW191_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9623734.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(26840..27145)
                     /locus_tag="VW191_000948"
     CDS             complement(26840..27145)
                     /locus_tag="VW191_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEU9623735.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(27161..27580)
                     /gene="ruvX"
                     /locus_tag="VW191_000949"
     CDS             complement(27161..27580)
                     /gene="ruvX"
                     /locus_tag="VW191_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU9623736.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(27584..27850)
                     /locus_tag="VW191_000950"
     CDS             complement(27584..27850)
                     /locus_tag="VW191_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEU9623737.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(27937..28506)
                     /locus_tag="VW191_000951"
     CDS             complement(27937..28506)
                     /locus_tag="VW191_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9623738.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(28572..28970)
                     /gene="spx"
                     /locus_tag="VW191_000952"
     CDS             complement(28572..28970)
                     /gene="spx"
                     /locus_tag="VW191_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEU9623739.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         8128 a         6488 c         5383 g         9053 t
ORIGIN      
        1 tatagtatac acatctaatc ttaaatagaa cttaaaattt ctcctatttt cttaattttt
       61 aaaactatga attggtgctg ggatttgtcc accgcgagag atgaagtcga cagacgaagc
      121 cccattaacc ctcataacgg gtgcagttcc taatagacca ccaaactcaa tcatatcgcc
      181 ttcttttcct ttgggaatga tacgaacagc tgttgttttc atgttgataa caccgattgc
      241 tgcttcatcc gcaatcatag ccgcaatagt ttcagcaggc gtatcttctg ggatggcaat
      301 catatccaat ccaacagaac agatagccgt catagcttct agtttttcta aattaagaga
      361 gccattttgc actgcagcaa tcattccttc atcctcagaa acagggataa aggcaccaga
      421 taaaccaccg acctggttac aggccatcac tccacccttt ttaacttggt cgttcaagag
      481 ggccaaggca gccgtcgttc catgcgtgcc aactgtttct agccccattt cctcaaggac
      541 acgtgccaca gagtctccaa ccgcaggggt tggtgccaaa ctcaagtcca caataccaaa
      601 ctccacaccc agtctctcac tggccatttg accaaccaat tgaccgatac gagtgatttt
      661 aaaggcagtt ttcttaactg tttcggctac tacatcaaag ctctgtccac gaactttttc
      721 caaagcacgt ttcacaacac caggaccaga aactccgaca ttgatgataa catctgcttc
      781 cccaacacca tgaaaggcac ccgccataaa tggattgtcc tcaacagcat tagcgaatac
      841 aaccaacttg gccactccca tatctgaaag atttgctgtt tccttgataa ttcgtcccat
      901 atctgccaca gccgtcatat taataccaga cttggttgag ccgatattga ctgacgagca
      961 gaccttatcc gtctcagcca aagcgcgagg aatggaattg atgagaatct catctccctt
     1021 ttgataacct ttttgtacta aggcagaaaa accaccaata aagtccacac caatctcttt
     1081 cgcagcctta tcaagcgctt ttgccagaac cacgtagtcc gtcgcatctg tcgctgcccc
     1141 aatcagagaa ataggtgtca ccgatacacg cttattaacg ataggaattc ccaactcagc
     1201 cgcaatttca tcaccaacag ctactaaatt agccgccttt gtcgtaattt tttgatagat
     1261 tttctccgca gcacgattga tatctggatc gatacagtcc aaaagagaaa tccccatggt
     1321 aatggttctg atatcgaaat tttgctcctc aatcatggcg atggtttcag taacttgtct
     1381 aatatccatg ataacctcct agatattata catagcttcg aaaatcgctg cactctgaat
     1441 attgattttt acattcaaag tttgcccaaa agcttcaaat tcattacgaa gataggtaaa
     1501 atcttgcttt tcatcactag atacaatagc catcatcgta aaatattcat ccaagacagt
     1561 ttgagagata tcgtcaatat tcaatcctaa ttctgcaatt ttaccagaaa cacctgcaac
     1621 aattccagat ttatctttac caacaacagt tataatcgct ttcataagca aactccaatt
     1681 cttcttaaaa taagaaacct taacattaaa caggattctt ttcaaatagt atagcataat
     1741 tctaacgaaa atactcaaaa acgcctgctt acgaagttgt tcttaagaaa aaaacaattt
     1801 cgtaaacaag cgtctactat cccaacttat gatgagtgtt cccgctagga ccgaaatcct
     1861 agcggtagac cagagctaga ctaagagcac aagactccat catcataaca ctcaacaaaa
     1921 ttgatgattt tatactaatt cgatgatcgc cattggcgct gcatcaccac gacgtggttc
     1981 agttttaagg atacgagtgt atccaccgtt acgttcagca taacgaggtg cgatttctga
     2041 gaacaatttt tgaagtgctg tagtagaagt gtacttatca gttgcttcat catagttttc
     2101 agatgcgatt tcattacgta cgaaagcagc tgcttgacga cgtgcatgca aatcaccacg
     2161 tttacctaga gtaatcattt tttcaacagt tttacggatt tctttagcac gagcttcagt
     2221 tgtcacgatt gattcgttga tcaaaaggtc agttgtcaaa tcgcgaagca ttgctttacg
     2281 ttgtgagcta gtgcgtccta gtttacggta agccatgtat tcctccttta tttatctttt
     2341 aatccaagac ccaaatcaat gagtttgagt ttcacttctt ccaaactctt gcgtccaaga
     2401 tttcgtactt tcatcatctc tgcttcagat ttttctgtca aatcatgcac agtattgata
     2461 ccggcacgtt ttaaacagtt gtatgaacgc acagacaagt ccagttcctc aatcgtacga
     2521 tctaaaatac ggtcgtcaga ttcagtatca gcttctttca tcacttcagt tgacttagca
     2581 atctcagtaa gatttgtaaa caaatcaaga tgttctgtca aaatacgtgc tgaaagccct
     2641 aaagcatctt ctggaataat tgttccattt gtcaagattt caagggttaa tttgtcgaaa
     2701 ccatcattgc tacctacacg agcaggttcc acttgatagt tgacttttgt aactggtgta
     2761 taaatagaat ctacagcaag tgttccaact ggtgcattat cctttttatt ttcatcagca
     2821 ggtacatatc cacgaccact gttaacagtc atagtcgctt ttagagaaga accttcaccg
     2881 attgtaaaga gataatgatc tggatttaca atttcaatat cgctatctgt caaaatgtca
     2941 ccagctgtta cttcagcagg accttcaaca tccagttcga tgattttttc gtcttcaacg
     3001 tacgatttca ctgcaattcc tttaatgttc agaatgattt gcatcacgtc ttcacgaaca
     3061 cctggaactg tgtcaaactc atgtaacaca ccatcaatgt tgatagatgt cacagctgct
     3121 cctggtagag aagctagaag tacacgacga agagagttac caagagttgt accgtagcca
     3181 cgttcaagtg gttcgattac aaacttgcca taatctttat tttcatcaat ttttgttata
     3241 tttggttttt caaactcgat catttagtta ctccctctta aacgaaaagc agtgtaatgc
     3301 gatgattata cacggcgacg ttttggagga cgagcaccat tgtgtggcac tggagtcaca
     3361 tcacgaattg ctgttacttc aagaccagcg gcagcaagcg cacgaatagc tgactcacga
     3421 ccagaacctg gaccttttac agtaacttca actgatttaa gaccgtgttc ttgtgcagat
     3481 ttagcagcag cttcagaagc catttgagca gcgaatggtg tagatttacg agaacctttg
     3541 aaaccaagag caccagctga tgaccaagca attgcattac catgcacatc agtaatcata
     3601 acaatagtgt tattaaatgt agcgtgaata tgagcaatac cagattcgat attctttttc
     3661 acacgacgtt tacgtgttgg tttagccaag acttttacct cctatattat tttttcttac
     3721 cagcaatcgc aacagcttta cctttacgag tgcgagcgtt gtttttagtg ttttgtccac
     3781 ggacaggaag tccacgacgg tgacggatac cacggtatga accgatttcc atcaaacgtt
     3841 tgatgttcaa gtttacttca cgacgaaggt caccttcaac tttgattgca tccacttcac
     3901 gacggatagc atcttcttga tctgatgtaa gatcacgtac acgaacatct tctgagattc
     3961 cagcagcagc caaaattttc ttagatgttg caagtccgat accataaaca taagtcaatg
     4021 agattactac gcgtttgtca tttggaatat caactccagc aatacgagcc atgttttctc
     4081 ctttctatct tatccttgac gttgtttgtg ttttggattt gctgggcaaa ttaccataac
     4141 acgaccatta cgacgaataa ctttacagta ttcgcaaatt ggtttgaccg atggtcttac
     4201 tttcatttct tatccctcca agtttttcga ttatttaaag cggtaagtga tacgtccacg
     4261 tgtcaagtca tatggactca tttcgacagt aacacgatct cccgctaaaa tacgaatata
     4321 gtttttacga attttaccag aaactgttgc taaaatctga tgtccatttt caagttcaac
     4381 cgtaaacatt gcattcggca ttgtatcaac tactttgcct tcaacttcaa tcacatcgtc
     4441 ttttgccacg caaaagcacc tccataaatt tcgattcgat gcctctagac acagagacaa
     4501 caattataag tcagactatt tcagtataac atttgtagcg gatttttgca agtgtgaaaa
     4561 acgctttatt tcaaatttgt caatactttt tcaatatctg agaagacatc attgatatct
     4621 tgattacctt cgatgtcatg aaccaaacct ttggcacggt agtgagcaat gattggttct
     4681 ccttgagcaa tattaacatc caaacgacgt tttactgtct caggcttatc atcttcacgt
     4741 tggtagtaat cttcttcttt atagtcaact ggtgggttaa agaccttgtg gaaagtttct
     4801 ccagttacgc ggtggatgat acggccactc aaacgttcca agaggctgtc agggttcact
     4861 tcaatattga taataccttc tagttcaatg ccaagttcag ccaatgtttt gtccaaggca
     4921 tgagcttgtt caattgtacg tgggtaacca tccaataaga atcctgtttc tttaatatca
     4981 tcttgtgaaa ggcgttcttt tacgattcca tttgtaactt cgtcaggaac caattcaccc
     5041 ttgtcaatat atgacttagc aagaacaccc atttcagttt gatttgccat tgcagcgcgg
     5101 aacatatcac ctgttgagat atgtgcaaca tggaattgtt ctacgatttt tgctgcttga
     5161 gttcccttac ctgcaccagg taagcccata atcaaaagat tcatgatttg atctccttat
     5221 tttgttttta aatagaagca aaaagttttt tcaccccgat ttaaaaacaa aatagaagag
     5281 gggagatcca actcttacaa atgtcagagt ttaaacctcc actccctcag tatttactga
     5341 ttcagtaaat acttttattc tgttctgtcc atgaaaccaa catacttacg tttcaatagg
     5401 taaccttcca attgcttgat tccttcgata cctgtagaga taatgatcaa gagacttgtt
     5461 ccaccaaagg caacaacatc agaaagacca aatacatctt tagctgcaat cggtaaaatg
     5521 gaaatcacac caaggaagag ggaaccaaca gttgcaagac gacgaagaag tttagacata
     5581 tattcttctg tacctttacc aggacgaact ccatggatat aggcaccact cttttgtagg
     5641 ctctctgctg ctttttcagg attaatctgt acaaacgtat agaagaatgt aaagagaata
     5701 atcaacaaag catacatggc aataccagtt ggagaagtag ttgccaacat ctcttgtgct
     5761 acccttaccc aagcccaatc atgacctgtg gcactcaaaa actgaagaat agccgcaggc
     5821 gctgcagtaa tcgaactggc aaagataaca gggataactc cagcaggatt tacctttaac
     5881 ggaaggtaag agctagatgg agcaccttgt gcaaccttag tatattggat tggaattttg
     5941 tattctgctt gttgaacata agttgtaaag taaataatca acaatacagt aataatcaaa
     6001 atgattacga aaatgataga tgaagtgata cggctacttg ggacgttcac aaagtagtcc
     6061 acatagatgc cctgaatcat ctctggaatt gaggaaacaa tcccggcaaa gataatcatg
     6121 gaaacaccgt ttccgtatcc cttatctgta atttgctcac ccaaccaagt gacaatcata
     6181 ctaccagctg ttaagatgat accaatcgtc agaaaaactt gtggagttaa agcagtttta
     6241 atcaattgag ctccagccaa ggtattaaaa ccagctgtaa tcccgataga ttgcacaaaa
     6301 gcgagaacta gagcaatata acgagtagct tgattcaatt ttcttcgacc tacttcccct
     6361 tgtttacccc actctacaaa cttgggtaaa atatccattt gcaagagttg gacaacaata
     6421 gaagcggtga tatagggact aactcctagg gcaaaaatcg aaaagttttt tagggcattc
     6481 cccgacacca agctcaacat gtttaagaag gataatccac ttaaagcatt caagctattg
     6541 gcattcacac caggaactgt aatgctagtt ccgatacgaa agaccaaaac gataaaaatt
     6601 gtaaataaaa tttttgatcg aacctgcttg actttaagag cttctcttaa taatttaaaa
     6661 aacataggtc acctctctta gatgacttct actgaaccac ctttagcagt gatagcttct
     6721 tcagctgatt tagagaattt agctgctttc acagtcaatt tcttagtcaa ctcaccgtta
     6781 ccaagaattt taatacctga cttttcagct ttaacaattc ctgcttcgat aagaacaact
     6841 ggagttactt cagcaccatc ttcaaagacg ttcaattggt caaggttcac aattgcgtat
     6901 tctttagcgt tgatgttagt gaatccacgt tttggaagac gacggaacaa tggagtttgt
     6961 ccaccttcaa aaccaaggcg aactccgcca ccgctacgag ctttttgacc tttttgacca
     7021 cgaccagatg ttttaccgtt acctgatgaa gtaccacgac caacgcggtt acgtacttta
     7081 cgagaacctt ctgcaggttt caattcatga agtttcattt ttattttctc ctcttttgta
     7141 aaatgctagc gccgataagg gagaaaaggt tgtctccccc atcaactcgc ctatacgatg
     7201 tcatcataga tgactatatc tagttttagg ggatggtaca gtgcacatcc cctaaaactt
     7261 cattagttta cttcttcaac tgttactaag tgagatactg ctgtaatcat accacggata
     7321 gcagcgttat cttctttaat aacagagctg ttcaatttgc caagtccaag tgctacaaca
     7381 gttttacgtt gtgatggaat gcgtccgatt ggagacttag tcaaagtaat tttaatttga
     7441 gccattttat cccctttctt atgccaaatc agaaactgaa ataccacgaa gggcagcaat
     7501 ttcttcagcg cgtttcaatt gtttcaaacc ttcaacagtt gcacgaacaa tgttgattgg
     7561 agtgttagaa ccaagtgatt tagatgtaat atctgccaca cctgccaatt ccacaacggc
     7621 acgaactgca ccaccagcgg caactccaga accttctaca gcaggtttca acaatacttt
     7681 agctccaccg aattctgaaa gaacttcgtg tgggattgtt gttccaacca taggaacttc
     7741 gatcaagttt ttcttagcat catctactgc tttacggatt gcttctggaa cttcttgagc
     7801 tttaccagta ccaaatccta cgcgaccatt gtggtcacca acaacaacaa gagctgcgaa
     7861 acgaagacga cgtccacctt taacaacttt tgtaacacgg ttgacagcaa ctacgcgttc
     7921 ttctaattca actgcattgt ctttaaatgc cattttctag tgtcctccta ttagaatttc
     7981 aatccgtttt cacgagctgc atcagccaaa gctttcacac gtccgtgata tagatatcca
     8041 ccgcggtcga acaccacttc tgaaatacct ttagcgtttg cacgttctgc aacgagttta
     8101 ccgacagcaa cggcttgttc agttttagtt ccttttgaaa cttctttatc aagagttgaa
     8161 gcacttgcga gcgttacacc cgctacgtca tcaatcactt gagcgtagat gcctgtatta
     8221 gaacggaata cgttcaaacg tgggcgatca gcagttccag agagttttcc gcgaacgcga
     8281 cggtggcgtt tttggcggag tttgttttta tctggttttg aaatcacagt tttcacctct
     8341 ttagttttaa atcgtgtgct atgcacaaag ttggaaaata ggttggtggt tgatgatcaa
     8401 ccactcaaca ttatttacct gttttacctt ctttacgacg aacgaattca ccaacgtaac
     8461 ggataccttt acctttatat ggttctggtg aacgaaggct acgtacgtaa gcagctgttt
     8521 gaccaactac ttcttttgaa attccgctaa caacgattgt tgttgggttt ggaagttcaa
     8581 aagtaattcc ttctggagct tcaacttcgt ctggatgaga tttaccaaca gccaaaacaa
     8641 gtttagatcc ttgaagctgt gcacggtaac caaccccacg catttcaagt tctttcttga
     8701 atccttctga tacaccaaca accatgttgt tcaaaagggc acgagtagtt ccgtggatag
     8761 ttttcatttc ttttgaatcg tttggacggt gaagagttac ttcagtacct tccacacgga
     8821 tttcaatatc ttttgagaac tcacgagtaa gttctccttt aggtcctttt acagttacaa
     8881 cgttgtcatt gttagcgagt tcaacaccag caggcaacac gataacttta ttaccaatac
     8941 gtgacatgtt tattttctcc tgttaaattg tcaggccaaa acggccagtt ttcacggggt
     9001 tcagatactt atttagttca agagctgaac tgaacacgct ctaagaactg tgcaaaatag
     9061 ataagttggc ttgtttgtaa acaaacttct ccaacttcct aattttgctt tgttctttac
     9121 gagctttgta tcttgatttt accaaacgta agcgataacc tcaccaccaa cattcttttg
     9181 gcgtgcttct ttatcagtaa gcaaaccttc agaagttgaa aggatggcaa ttccaagtcc
     9241 gttaagaact tttggaaggt cttcacgttt tttgtagaca cgaagtcctg gtttagaaac
     9301 acgtttcaag ttagtgataa ctttttcacc atttggtccg tatttaagaa atacacggat
     9361 gacgccttgt ttgtcatctt cgatgatttc aacgtttttt acaaaacctt cgcgtttaag
     9421 gatttcagca atcccttttt tgatgtttga tgcaggtact tcaagtactt cgtgtttagc
     9481 ttggttagca ttacgaatac gagttaggaa gtctgcgatt gggtcagtca taaccatttt
     9541 gtttttctcc tcttactagt agtttgcaag ttgcacttgc tagttaatac atgatacaaa
     9601 gagcgtaaca gcacgagcaa aaatagacaa tttaatgcag aagcaatgct ccaaagcaaa
     9661 ttggtctttt ttgccaaagc tgtagctcgt gttcaaattg gcaagcccaa ttgaacccgg
     9721 gctaaactct gtgtgaaaaa gataaacttt cctagaaact ttagtttctt cgtcaagttt
     9781 cctattttta cttggagttt tgacgccctt gatatcttaa attaccaaga tgcttttgtt
     9841 acaccaggaa tttgaccttt atgcgcaagt tcgcgaaaag cgatacgact cagaccaaat
     9901 ttgcggtaaa ctgaatgtgg gcgccccgta actctacaac gattatgtaa acgagtcggt
     9961 gaggcgttgc gaggtaattt agataaacct tcgtagtccc ctgccgcctt taatgcagca
    10021 cgtttttcag cataacggtc aacaattttt tggcgtttag cctctctagc taccattgat
    10081 tttttagcca ttagatttac ctcctatatt attttgcaaa aggcattcca aggcctgtaa
    10141 gcaatgcacg tgactcttcg tcagtgttag cagttgttac gataacgatg tcaagaccac
    10201 gagttttgtc aacgtcatcg aagttgattt ctgggaagat taattgttct ttcacaccaa
    10261 gtgtgtagtt cccgcgtcca tcaaatgatt ttgttggaac accgtggaag tcacgtacac
    10321 gtggaagtga aactgatacc aatttatcca agaattcgta catacgttca ccacgaaggg
    10381 taacttttga accgatcgca acaccttcac gaagacggaa gccggcgatt gattttttag
    10441 ctttagtgat aagtggtttt tgacctgaga taagtgccaa ttcttcagca gctttttcaa
    10501 ggcttttagc gtttgataca gcttcaccaa cacccatgtt caaaacaatc ttatctactt
    10561 taggcacagc catcactgat gagtagttga attgttctgt caaagcagga actacttcat
    10621 taagatattt ttcttttaaa cgatttgcca ttatacttct cctttccttc gtgattaatc
    10681 aagcacttcg cctgattttt tgttgtagcg aactttttta ccgtctacaa atttgtaacc
    10741 aacacgacca gctacaccat ttttgtccaa aacttgaacg tttgatacgt ggatagctgc
    10801 ttctttctcg atgataccac cttgaggaag ctcgttagtt ggacgttggt gtttcttaac
    10861 aatgttaaca ccttcaacga taactttgtt tacttttgga agggcagtaa ggacaacagc
    10921 ttctgttccc ttatctttac cagcgattac gcgaactttg tcgccttttt ttacaaacat
    10981 taggtttctc cttgattttt cttacgccca taagggcacc ctagcttgaa gctaggggac
    11041 tagtttgttc ctaaaaatta aagtacttct ggagcaagtg acacgatctt catgaagcca
    11101 ccttcacgca attcacgtgc aactgggcca aagatacgtg ttccgcgagg ggttttgtct
    11161 tcacggatga taactgctgc gttttcgtca aatttgatgt atgaaccatc agcacgacga
    11221 gcacctgatt tagtacgaac gataactgct ttaacaacgt cacctttttt aaccgcacca
    11281 ccaggagtag cttgttttac agatgccacg ataacatcac cgatgtttgc aaatttacgt
    11341 cctgaaccac caagaacttt gatagtcaag atttcgcgag caccgctgtt gtctgcgact
    11401 ttcaaacgag tttctgtttg aatcatttca gttttctcct ttcaggtttg attagatgat
    11461 gaccgcttct tcaacaactt ctacaagacg gaaacgtttt gtagctgaaa gcgggcgagt
    11521 ttccatgata cgtacgatat cgccttcttt ggcaacattg ttttcatcat gagctttgta
    11581 ttttttagag tagttaatac gtttaccata gactgggtgg ttacgttttg tttcaactac
    11641 aactgtgatt gtcttgtcca ttttgtcaga tacaacacgt ccaacaagaa ctttacgatt
    11701 attgcgttcc attgaaattt ctccttccct agtctattat ttcgcttcag attgaactgt
    11761 tttgatacga gcgatttgtt ttttaacttc tttcaagcga gctgtttgtt ccaattgacc
    11821 agtagcagct tggaaacgaa gttcaaacaa ttcttttttc aattcgtttt cgcgcttcgc
    11881 gagttcttct tgagaaagac cacgaagttc tttaacaaat tcttttactt cattaagttt
    11941 catgccttct ccttattctg cttcacgttt tacgaattta catttaactg gcaatttgtg
    12001 gctagcaagt cgaagcgctt cacgtgcaat ctcttcagat acaccagcga tttcgaacat
    12061 cactttacca cgtttaactg gtgctaccca accttcaggt gcccctttac cagatcccat
    12121 acgcacaccg atagctttag cagtgtatga tttgtgtggg aagattttaa tccaaacttt
    12181 accaccacgt ttcatgtaac gagtcatggc gatacgagca gcttcgattt ggcggttagt
    12241 gatccagtgg ctagttgtag cttgaagacc gtattcaccg aatgctactt cttttccacc
    12301 ttttgcttca ccgcgcattt ttccacggaa ctcacgacgg tgtttaacac gtttaggtac
    12361 taacattggt tatttacctc ctttagtgtt tttacgagct ggaagaactt caccacggta
    12421 gatccatact ttaacaccaa gtttaccgta tgtagtatct gcttcttccc aagcgtaatc
    12481 gatatctgca cgaagtgtgt gaagcggaac agttccttca gagtatcctt cagcacgggc
    12541 gatatctgca ccgttcaaac gacctgatac ttgagttttg attcctttag ctccagcacg
    12601 cattgcacgt tggattgctt gtttttgtgc acgacggaaa gcaacacgtt gctccaattg
    12661 acgagcaatt ccttcaccta caaggtgagc atccaaatca ggttgtttga tttcgatgat
    12721 gttgatgtgt acttgttttc cagtcaattt gttaagtttt gcacggagtg catcaacgtt
    12781 agcaccacct ttaccgataa ccatacctgg tttagcagtg tgaagtgaaa cgttaacttt
    12841 gtttactgcg cgttcgattt caatagttga aactgctgcg tcagcaagtt ctttttgaac
    12901 gaatttacgg attgcaagat cttcatgaag gtaatccgcg tattcttttt cagcatacca
    12961 tttggcatcc caatcacgga tgatgccgac acgcatacca attggatgta ctttttgacc
    13021 cacgatttta cctccttatt tttctgcaac agctacagtg atgtgagctg tacgtttgtt
    13081 gattggtgaa gctgaacctt tcgcacgtgg acggaaacgt ttcatagttg gtccttcgtt
    13141 tgcgaatgct tcagatacta ccaaattagc tttatccaaa ccaaagttgt tttcagcgtt
    13201 agctacagct gagttcaaaa ctttcaagat gatttcagca gctttgtttg gagtgaatgt
    13261 caagattgcg attgcatcgg ctacgctttt accacggatg ttatcaagaa caagacgtga
    13321 tttacgaggt gaaacacgta ctgtacgagc cattgcttta gctgaagtaa tttctgccat
    13381 ttatgttctc cttattttct acgtgttttc ttgtcgtctg cagcgtgacc tttgtaagta
    13441 cgagttggtg caaattcacc aagtttgtgg cctaccatgt cttcttggat gtaaacaggt
    13501 acgtgtttac gtccgtcata aactgcaata gtgtaaccaa tgaaacttgg gaagatcgtt
    13561 gaacgacgtg accaagtttt aataactttt ttcttttcgt cgttagcttg agcttcaact
    13621 tttttcatca aatgctcatc gacgaaaggt ccttttttaa gactgcgtcc catttttata
    13681 ttttctcctt taaatgttgt accacagcgg cttgcactcc tatggagcgc taccgagctg
    13741 gcggatttac tagttgctta agcgactagt ttaatattat ttctcgttgc gacgacgaac
    13801 gataagtttg tcagatttcg ctttcttgtt acgagtttta agaccaagag caggtttgcc
    13861 ccatggagta gatggtgctt tacgaccaac tggtgcttta ccttcaccac caccgtgtgg
    13921 gtgatcgtta gggttcatta cagaaccacg aactgttggg cggatacctt tccaacggct
    13981 acgtcctgct ttaccaaggt ttacaagtcc atgttgttcg tttccgacaa caccaactgt
    14041 agcacggcaa gttccaagaa tcatacgaac ttcacctgat tgaagacgaa caagaacata
    14101 tttaccttca gaacccaata cttgagcaga tgcaccagca gcacgtacca attcaccacc
    14161 acgacctggt ttcaactcga tgttgtgaat caaagtacca actgggatgt tagcaagtgg
    14221 aagagcgttt ccgactttga tatctgcttc tggacctgaa acgatacgtt gacctacttc
    14281 aagacctttt ggagcgatga tgtatgcttt cacaccgtca gtgtagtgta caagagcgat
    14341 gtttgcagaa cggtttggat cgtactcgat tgttttaaca actgcttcaa cgttgtcttt
    14401 attacgtttg aagtcaacca aacggtaaaa acgtttgtgt ccaccacctt ggtgacgaac
    14461 tgtgatacga ccgttgttgt tacgaccagc cttgctcttc aatgcaacaa gcaatgattt
    14521 ttcaggagtg cttgttgtga tttcagcgaa atccaaagaa gtcatattac ggcgaccgtt
    14581 tgttgttggt ttataaacac gaattcccac gatatttcct ccttagatta ttcagcttca
    14641 gcagcaaaca actcgattgc tttagaatca gctgtaagtg tgatgatagc ttttttagtt
    14701 ttgttagtaa aaccagtgta acgtccaaca cgtttagctt ttggttttac gttgattgtg
    14761 ttaacattgg caactttaac accttcgaaa gcagcttcaa cagcttgctt gatcaaaagt
    14821 ttgtgtgcac gagtgtcaac ttcaaataca tattttcctg cttcaagttg agccattgag
    14881 ctttcagtga tgacaggttt tttgataaca tcatacaaat tcattatgca agaacctcct
    14941 cgattttaga gatagctgct tgtgtgacaa gaagtttgtc gctatttgcg atgtcaagaa
    15001 cacttgcagt tgtagcagtt gcaactttca cgtttggaag gttacgagct gaaagagctg
    15061 cgaattcatt tccttcttca aggataacaa gaactttaga atcgatgctc aatgctgcaa
    15121 gaacttttgc aaattcagca gtttttggag ctgtaaatga aagagcgtct acagctacga
    15181 atttgttttc agcaactttt tcagagtaaa ctgatttaag agctaggcga cgaacttttt
    15241 gtggaagttt gtagccgtat gaacgtggag ttggtccgaa gacaacacca ccaccacgcc
    15301 attgtggtga gcggatagaa ccttgacgag cacgtccagt tcctttttga cgccatggtt
    15361 tgcgtccacc acctgatact gcagagcggt ttttaacagc gtgtgttcct tgacgaaggc
    15421 ttgcgcgttg gctgatgatt acatcaaaca caactgattc atttggttcg ataccaaata
    15481 ctgcatcgct aagaacaact tggccagctt ctttaccagt ttggtcaaat aatgttacgt
    15541 ttgccattgt gactgatttc ccctttcttt attatttacc agctttaact gctgatttga
    15601 tagtgataag agatttctta gcacctggta cgttaccttt gataaggata acgttctttt
    15661 ctggaacaac ttgtacaact tcaaggtttt gaattgttac gcggtcgcca cccatacgtc
    15721 ctgcaaggtt tttacctttg aatacgcggt taggtgcaac aggccccata gaacctggac
    15781 gacggtggta acgagaaccg tgagccattg gtccacgtga ttgtccgtgg cgtttgataa
    15841 caccttggaa acctttacct ttagaagtac ccgttacgtc aacaacgtct ccagctgcga
    15901 atgtttcaac tgtaatttca gcaccaactt ccaagccttc aacgtttttg aattcacgaa
    15961 tgaagcgctt aggagccgtg ttagctttcg ctacatgtcc tttagcaggt ttgttgctca
    16021 atacttcgcg tttgtcatcg aaaccaactt ggatagcgtt gtatccgtct gtttcaacag
    16081 ttttaacttg aagaacaacg tttggagttg cttcaataac tgttacaggg atcaattcgc
    16141 cagcttcagt gaagatttga gtcattccca cttttttccc taagattcct tttgtcatga
    16201 gaaaatagtt ccttttctat attttttatt caaaaagttt ttaacgagcg ttttttatgc
    16261 tcaagtctaa gatacaaagg gcgtcaaact caacgtgaaa ataggaaact gacgcagtgt
    16321 ctagcagaca ctaggaagtt tatctttttc acaccgagtt tagcccgtgt tcaattagat
    16381 tgaaacacca gcctctgctc ttttatattt tagattaaag tttgatttct acgtttacac
    16441 cacttggaag atccaatttc atcaaggcat caactgtttt ttgagttggg ttaacgatat
    16501 cgatcaaacg tttgtgtgta cgcatttcaa attgttcgcg agagtctttg tatttgtgag
    16561 tcgcacgaat gattgtgtag aggctacgtt cagttggaag tgggattgga cccgcaactt
    16621 gtgcacctgt acgagtagct gattctacga tttttgcagc cgctgtgtca agcgtacggt
    16681 gttcgtaagc tttcaaacgg atacggattt ttttgtttgc catctttttc tccttttcgt
    16741 ctatttaaga taataggcta gctccacaag aaaaccgacg cggtgttgcg tggcaatgca
    16801 accgagcgtg tcgcaacctc ttgcatcaaa actaaggctg taatttacag caccataata
    16861 gaataacaca aagcccctgc gattgcaagg gatttgttgc tgttttttcg tttttttggg
    16921 atgaaactgt tttcctactt tttctttcta aaattaggga ttttcttcta tatttcttct
    16981 atacataagt taagtcctct tgacatcaaa tttatcttct gattggtcca ctaagatatc
    17041 cgccttagac tcagtttctt tatagtatcg cagatactgc tcccgtctca tctgatggct
    17101 agccaatata aaggatgcat cgcgatttct cacagtcgta tctctagcaa ggcgtcgctt
    17161 taattcggtc tcctcatccg tgtagaaaca gatggttttt tcaaagagtt ccttgggtag
    17221 aaagccaaca gacatccctt cgacaatcaa aattggtttg gctccagaca agacctcact
    17281 agccttccaa ggttcttcaa ttgtcaagac atccataccc gcctgcaagg caaggatatc
    17341 tctctgcaaa ctctccagtt catgcgccac tggcagactg gctgtcacct tttgattcgg
    17401 cgcgtccttg ggtactacca gatggcgtcc tgaggtgata taaggatctg tctctagcaa
    17461 atttactgta gtagaatcta aagcttggta caattcctgt gcaaaggttg atttacctga
    17521 agctccatga ccgtatattc ccagtgtcct gactttcccc gtctcgatct ctgagactag
    17581 ttggtctact aagtcttttt tcttcattct ctcttcacct gttcatagct ttcttttccg
    17641 tcattgagct tgtcccaaat cactacttgc ccacttttga gcgatttttg cacatcgata
    17701 attcgttggt tggacgaacc tcgaaactgg agcatgagat ttctcttagt tcgatcatat
    17761 cttccatcga caagaatgtc aatcaatgat aagagttcca gtttatctgg agtttccaac
    17821 atcatttctt cccaagtgta gcccgtccag gaccaaatgt ccttgtctgg caattccttt
    17881 cggatgcgtt taactagagg caagagaatg ccagtattga gaaaaggctc ccctcccagc
    17941 aaagtcaagc cttgaacata gggttgggca aggtctgcca taatctgctc ttctaattct
    18001 gctgtatagg gaatgccagc attaaaagac caagtcgcaa cattataaca tccctcgcag
    18061 tgaaacatac agcctgatac atagagagag ttgcgcacgc cttcgccgtc cacaaagtta
    18121 aaggccttgt agtcaatgat acgaccttga ctaagttcct cgcttttcca ttcttgtggt
    18181 tttggattat tcattcgcta cctctatcca ataacgctcg actccattgc gagcatcctc
    18241 aaatattcca ccatttgcta gaatgactgc tctgctagca ggattattca cgctacaggt
    18301 caccagagct ttcttgatgt tcttttcctt agcaacttgc aagccctgac ggagagtctc
    18361 ttttgcataa cccttgcctc tttcagatgg acgaatggag tagccaatgt ggccaccttc
    18421 ttctagtaga aagttactga ggcgcaaccg gagattaaga aatccaactg cttgaccttt
    18481 ctcagaaaaa gccactaact gaattgcagg aacccatcct tcaggcagat taatccccat
    18541 ttcctgttcc tgattgcttt ctaaccagtc ttcatacaca aagttctctg tatcccagaa
    18601 accgccgtcg tgaggcgact gaaatttttc aaactctgtc atcatatcta aaacagcttt
    18661 cttatccgct aatcttggtc tgcgtaattc catccttacc tcccactatg agaaaagcga
    18721 gagctggctc tcacttttat tttttcttgt tcttgtttaa aaattgttct ctgaagctaa
    18781 gcaagcgttc tttcttacca gctccacctt tagagtgttt ctcgtgataa cgggtcactt
    18841 gtgcgcgccc cttatcgtct agttgatatt ttcccatttc atttctttct aatttgttac
    18901 ttgatgccca gctattttaa tcgttgaacc attcatatgt ttgacacgcg cagcgatttc
    18961 cttgtgacgc ccgttaacca ttggtctcgc ttgaggattg ccaagatagc cacaagtccg
    19021 tttaacaacg tctactgttt taggatcgct attgccacag tttggacaag caaaccctct
    19081 ctcagttggt tcaaaatccc cttcaaagtc acacttgtag caacggtcaa tcggagtatt
    19141 ggtgcctaga tagcctacac ggtcataagc ataatcccag acagcttcca aggcctttgg
    19201 attttgctga aggactggat actcacaata atggatgaaa ccacctgacg cacctgcttc
    19261 tggatagact ttctcaaagt ccaatttttc aaacggtgtt ggatttttac gaacatcgta
    19321 gtggaaagag ttggtgtagt attccttgtc tgtgatatca ggaatagagc caaacttgtc
    19381 tatatctagt cggcagaaac ggtctgtcag actttcggat ggtgttgagt agatagagaa
    19441 atggtagcca tattggtctg accactcttc tacacggcgt ttcatatcgt gaatgatgtc
    19501 tagcgtgaat tccttagcat ctggattact ttcccagctg ttaccaaaga aaactgtcgc
    19561 tacttcatac aagccgatat agcccagcga aacggtcgcc cgacgattct taaagagctg
    19621 gtcaacactt tcttctttac ctagacgatg gccaaaagca ccgtactgat aaaggatagg
    19681 agcattcgct ggtgtcgcct ctttagtgcg ttcgacacgg taaacaagag catcttctgc
    19741 gatattcatt cgctcgttga agatttccca gaacttattc atatcacctt cagactcaag
    19801 agcaatacga ggcagattaa ccgtcacaac acccagattc atgcgacctg aattgacttc
    19861 tacaccattt tcatccttcc acccttgaag gaaagaacgg cagcccatag gcaccttgaa
    19921 agaacctgtc aaatcaacaa tcttatcata agacaagacg tctggataca tccgcttggt
    19981 tgcacactct agagccaact gcttgatgtc atagttggga gttccttcct ctaagttgag
    20041 gcctctttta agcgtaaaga taagtttagg aaagatagcc gtacggtgtt ctgaaccaag
    20101 acccttgatg cgaatgttta aaatagcttt ttgaatttct cgttcaaaac gactggttcc
    20161 cagaccaaaa cctaacgaag taaaaggtgt ttgtccattt gaagtgaaga gagtattgat
    20221 ttcatactca agagattgca tggcatcgta gatgtccttt tgcgctttct tccaagcgta
    20281 atcttcctgt ttttcaggca atacccactc ttctgcatct ttgagatgtt tttgataatt
    20341 cttctctgca taaggcgcca aaatttcatc gatacggtca gctgaacagc caccgtactg
    20401 gctagaagca acgttggcaa tgatttgaga aatctgtgct gtcgcagtct ggatagactt
    20461 gggactctct acctctgcat ttccaatctt aaaaccattt tccaacatac ccttaaaatc
    20521 aatcaaacag cagttggtca taggggtata gggactgtag tccaaatcgt gatagtggat
    20581 atcccccttt tggtgggcat tggctacgtg cttaggaagc atttgcagtc cgattgattt
    20641 cccaacaatc cctgctgtca aatcacgctg agtgttaaag acatcactgt ctttattagc
    20701 gttttcattg acaactgtct ggtctttgtt gagaagttta tgaatactaa agttgatatc
    20761 cgtcgctttt gagcgctcaa aatccctctg tgtccgataa gtaatatact cctcagccag
    20821 cgcatattct ttggcttcaa ggagttcatg ttctacgata ttttgaattt cgtaaatctt
    20881 aattccctgt ggaaagcgac tatgaatttc tgtaataatt cgctcagtca gatcattgag
    20941 gcgtttttca accaggggtg tcacatccat aaccttgtca gccgccgtgt ggagagcctt
    21001 gtcaatcttg tccacatcaa atacaacacg tctcccatct cgtttctcga cgaagagagt
    21061 tggcaaaatt gtaatttttt cttctagtgc aatcatacgc atgctctttt ctaaaatgtt
    21121 ctttctaaaa agtattatac cactctaaaa agaaaaatca atatcttgtg ttaaaaatcc
    21181 taaaattttt aaaaatacac aagatattga tttggttatt taattttata gactttaaac
    21241 tctgagtaat cagtctttac ttgttggtaa ttttctttta agagtgtttc cacttcagac
    21301 cagactacca ccttgtcatt taccacaatc accttaggtt gtttttcttt caagtcatta
    21361 agtaacttat tacgattttc ctcagttgct gtatagtgca acggggacaa aatcgccgat
    21421 ggcgacaagc gctcactctt acgataaaga gtcgcagtat catcccatgc atagatagca
    21481 tcttccgtat ttgtctcctc ttttaccaaa tcagcaagac gattacgttc ttgataaggt
    21541 actggataaa ggacaaactt catcaaaaaa ggaacagaca aaagatagac aaacccaaaa
    21601 actagcagat atagatttcc tttgaagaaa cgactccata ggctaactgg cgtttctctt
    21661 cggcttcttc tagcactacg ccccctctgt tgactcttaa tattggttac caataaaagt
    21721 aggaaaacag ggaagacgac catcaaataa gaagcgtgca gaggctcttg tgaaaagaaa
    21781 agtaaaatca aacctaaaat taccacaaga cttgcaggaa ccgaaatgac gtatagttta
    21841 aagggtttgg attgaaagag acctaaaaag atacaaacca caaatcccaa accaagggac
    21901 aacaagccat aaaaaaacat attctctaaa attttagaag taaaatcaaa gcgaatactg
    21961 tcaataggat aacgaatacc actaatcgca tccccaaaac ttcctgttgc agcactatag
    22021 taggcagttg gataaaagac aagtgaaaaa cctaaagcca ctgcaagaaa ctgataaaac
    22081 ccatgcacaa aacgtctatg cccaaggtta aagaccaaca agcctaaact cactacagca
    22141 ataaacagga gcgatgataa gggagcaaag aaaaatccgc ctgccaaagc tagcccaatc
    22201 cgtacaaatc ccttatcatg gcttggattg cttaggtaag ccgcaactaa actaaaggct
    22261 gcgaataaga aaggaagcgc taaaagagtc gcatagcctc caccaaaagc aagacctgta
    22321 actagcatgt aaaaaatagt caccagatgt ccagcttggt ctccttgctc tgtcaaggtg
    22381 tccgctgatc taaaaaggaa aaatcctcct gctaccaagg ctaaccactc aaagatggca
    22441 aagaaaaatc cgccctgact cacgtaagtc agcaaataat aaagcaaacc ttgacttcca
    22501 taatagtcgc tgtaaatctt ccctgtctga tgaagcgccc aacctgcata aaaatcctgc
    22561 acttcttgtg cactcattaa gtcgagtaat agcggtactc ctagagttat ccccgttaca
    22621 agcgtactcc atagtaaaat tttcaccaaa ggaagacgac ttgattcacg atgatgcgat
    22681 tcttgttcga tttggtattc tagaggttca cgattctcct tatgaacttc ttctactcta
    22741 ccatacacac tcatatcgtt tctcctgttc aatttatctg tcttagtata tcaaaaagtc
    22801 ctaggattgt cagcttgcga tggctgtttg agtgattttt tgcatagttt cacaaaggtt
    22861 tcaatttccc gaaagcggtg taaatgtgtc tgtttgaact caatcatatc attcacttct
    22921 tccttagtta gcatgaattc ccctcctctt tctttctatt cgtaaaagac aagaaaaata
    22981 ggtctggttt gtatcctaag ttctagattt cttataagat aggggcgaat agctgggcga
    23041 tttcttttac aagcttttga taccagctcg ttttgataga atgaggatag acttcctgag
    23101 aatctgcaaa tatatttcga aaatctcggg cgatttccct tatagaagga gttttataga
    23161 gtaagactgc attttcataa tggtgtacca agctccggta gtcgagattg attgtcccca
    23221 ccaccgcaaa atcttcgtct accaacatct gcttactatg aataaaacct ggactatact
    23281 cataaatccg aacaccagca gaaagaaggt cgggataagc tcctctcgtg actaactgaa
    23341 tgaacttctt atctggtatg taaggggtga taattcgaac atcgacccct ctcatagctg
    23401 catttttgat tgtctctgtt aaatcataat ctataatcaa ataaggtgtc gtaatataga
    23461 ccgattctgt tgcttgattg attaaactct gataaacttt tttccctacc tgcgctcgaa
    23521 aaattggctt gggtccactt ccgtatggaa tggttaaacc gtcactcggg atagaatgat
    23581 tttctaaatg atattgatca aaatcactaa tttctcctcg attaatgtac caagtggtca
    23641 aaaataagcg tgtcagagct tttactgcta gtccgtctaa gcgaattcca ctatccttcc
    23701 aataaccaaa tctctcgacg tggttaatgt actcatctgc cagattgacc ccaccagtat
    23761 aggctatctg accatcaaca atcaatattt ttctatgatc tctgttatta taagccactg
    23821 tcaaacgagg aataacttta ttgaatttat gggcctcgat gcccagctga cgaagtcgat
    23881 gtgcataatc tcctgttaaa gtagccatac agccgatatc atcatagagc atcttaacct
    23941 ctacaccctg agctaccttt tgctctagta tatctagtat gcgattccac atcaaacctt
    24001 cttctataat gtaatattcc aagaaaataa atttctcagc ctttttaaga tcttctacca
    24061 tctttttcca catagcttct ccgttaggaa aaaatgtaga ggcagtttga tcatagatgt
    24121 cagcattggt atccatactc aataaggact tgatgactcc ataagctgcc ttgtcacttt
    24181 cttttaattt ctcttttagt agctggctat ttgcttcttg gaaatgcata gagcctagct
    24241 tcttcagttg tttgatttct tttttggaca atcgcctttc accaaacatc agatagagca
    24301 agggaccaaa tactggcaca aaggctacta acaaccaggt taccttattc tcaggagtcg
    24361 tattacggtt gactatcgaa atgattgtga tgatactcag tagaatgagg acagtaatcc
    24421 ataaaatggg agccatgcgt cctaaataaa gaaagagacc aaaaacaaga caaagttcaa
    24481 gtagcataat cgaaagacta aagccatact tggacatcaa taattgaaat ttcctatatt
    24541 tcatatcccc tccttgatag tttgaatgct aaaaacaagt aattgatact agtataacgc
    24601 aggtattcgg tagaaggcaa gtatttatga ttattttcta ggtaaaagta aaaaggctga
    24661 aaaatccagc cctttctact tgcatagtct tatttagtta aaacgatttg gtccgatggg
    24721 tctctatcca tatcatctac gacgatttga ttaccgttta cagtgtaaat tttgacatca
    24781 tcgccaataa tcatgcgttg atttgctgaa tcaaatgtaa cctgtttgac tttctgatcc
    24841 ccgtcagatt cgagctcagt ccatgtacca gtcgttccag ttaccactaa agtgatacgg
    24901 tctccgtcat cctgaccagt ataagtccca tcaatatcgg tcggttgagc aacaggttgg
    24961 ttttgtactg gagccgcttg attttggtta cctcctgcat ttgcagtatt ggtattctgt
    25021 tgagcaggct gttggacctg ctgagcaaca ggctgttgaa cctgttgagc tctttgtgaa
    25081 caagctacta agagactaaa acttgctaga gaagcaagag aaagtgtgaa tctttttaat
    25141 ttcatgatga atttcctttc tgctaccaat ttagagaaat tttctctaac cagcaattcc
    25201 cctagtataa caagttcaaa aaagggggtc aatttatctg ctcacggtcc agcaggtagc
    25261 cccgtacttc tgagataaaa tagagagacc ctgtaacgaa cagcaaatcc tgagcatctg
    25321 ctctttcttc aaaatcgctg ataaattctc ggtaagaaga aactatatcg taacctgtca
    25381 catccctttc gtccaaagcc ccctgatagt caaagccggt caccttgagt tccacctgag
    25441 gcaatttttc agtcaggtaa cctaacatcc cttgataatc cttccgtttc aaggctccaa
    25501 aaaggatttg aggtcgatag ccttcctgct ctttttcttt gataaactca accaagcgag
    25561 tcaaggcagg gaggttatga gcaccatcca agtaaatctg tgggcgaata cgctccaagc
    25621 gaccagccca atgggtctgt tccaaggcct ttcttacagc ctgttcatca acagcttcct
    25681 ttccttctcc cataaaaaga agaaaagttt gcaacgccaa ggctgcattc tcctgctgat
    25741 agactccttc taagcctatg ttcagctgtg aaagatttag taaagagctt gaaaaatcac
    25801 cattcagcat taaaaaatct tgacctgcct gataaaggtt aacagctaaa gattcggctt
    25861 ttttctgaca gacaagccta gcttctggag gcaatttcgc aatcactgcc ttcttaccag
    25921 ccttgaaaat accagctttc tgctctgcaa ttgcttctag actatcaccc aaggtttctt
    25981 gatggtcaag cccaatggag gtgatgacag caagctctcc agttaccaca ttggtcgtgt
    26041 caagtaagcc accaattccc acttctaata aaaccaaatc cacctcttgc tccctaaaat
    26101 aaagaaaagc aactaaggtc agcaattcaa aaaaggacaa ctgggcagga gtttgcagaa
    26161 gcgttttctc catctccttg acctgatcag tcaaacggat aaagtctgcg tctgctatag
    26221 gttgcccatt aatgcagatt cggtcattga tagagacgat atgaggggag gtaaaggtcg
    26281 caactttttt gccatgcccc ataaataatt ccctcataaa agcaatggta gatcctttcc
    26341 cattagtccc tgttacgtgg ataatagggt aagactgctc aggatttcct aacaaatcca
    26401 ccgcttgctg cattcggccc aaacctgatc gaaaattcaa accaatccga ctatggagcc
    26461 attcttctac ttcaaacata cacatctcct tgacaaaagt ccaatcaatt atcgcattaa
    26521 agtatggtta ctaataaaaa caaggccagg attttcgtcc cgacctctta cctggttagc
    26581 taataactag ctactatgaa tgtgaacgtg ggctaaaaat atccactaga tgttccaact
    26641 cttctcaatt tctaggagtt ggggtaaaaa tgttcactgg acgttccaac tcttccccat
    26701 ttctgggagt tgggctgata cagtctccca gacttaccag ttctctctta tttaaaggaa
    26761 ctgggctaaa aacatccact ggacgttcca actcttcccc atttctggga gttgggctga
    26821 tacagtctct cagactgtat cactcctcca taaagctgtt gaagacttct tcaatcatgt
    26881 tccattcgtc ttctgagtct tctgggattg gttgcaattc gccttctgtt ccatcttcgt
    26941 tttcgatgaa tgagtaagct tggatttcaa cttgtccgtc ttcgtcttct tctgcgttaa
    27001 ctggtactag aagaacatag tttttaccaa attcttcttt tccatcaatt gtcaaaagga
    27061 tttcaaacaa ggtttcattt ccttgctcat ctactagtgt gattagttca cgttcttcgt
    27121 ggtcgtggtt atgatcgtgt gacatagcct ctcctttata ttaaaatttt ctatctaaat
    27181 aattttgtaa aatcagctga gctgctaact tatcaatgac tttcttgcgc ttattgcgac
    27241 tgatatctgc ttgttcaatc aacatgcgct cagcagccac tgttgtcaag cgttcatcct
    27301 gatagtctac tggtaaacca aaaaactctt ctagctttgc tccgtaggct tgactagctt
    27361 ctacgcgcgg tccacttgta ttgttcatgt ttttaggcaa gcccactaca aatcgttcca
    27421 ccttgtaagt atcaaccaat tccttaacgc ggtcaaaacc aaattggcct tgttcttcat
    27481 ttatctggat gatttcaagc ccttgagctg taaaaccaag cggatcgcta atcgccaccc
    27541 ctaccgtttt tgaaccgacg tccaatccca taattctcat aggttataga tcgactcctt
    27601 gtcctttgag gtagtagcga accaattcct caacgatttc atcacgctca tacttacgga
    27661 tttgatttcg tgcattatta taacgaggaa cgtaggcagg gtctccactc aatacgtaac
    27721 ctacgatttg gttaattggg ttgtaaccct tatcgttcaa cgaagcataa acatctgtca
    27781 aagtttcgct aatttctttt ttattggaat cgtccaattt aaaacgtact gtttcttcag
    27841 taaatcccat tctaacaccc tctttcctta gaatagtacc attatagcat aattccttac
    27901 cttctacaat tcaggcagtc tatttatttg gattttctac tgttctgtcg caccatttgc
    27961 caatctgtct gaaatatatt tgcttggttc atttttcaaa agattttcca aaccaatatt
    28021 cttcagatgt tccaactggg aagccttctt gacatccaga acttgaaaat caaaactagt
    28081 cgttgtttga agttccgttg cgctcaatag ttttgtttca agtttgaaac ctaccaattt
    28141 acgagcttca atgatagact tatccttctc ctccgcttca agaagagctt tttgagtttc
    28201 ttccactcca tgttggtcaa tataagtctt caactcatca gaaactggac gtttttgttg
    28261 aatcgttaag ctcaaaaaag tcttgtcctt gtatgtaatg gtttgagttt gctggctacc
    28321 atcatctgac ttgggcaaga ccaaagtctt ggttacaact gtattcttct cggcattttc
    28381 aataactggc aatgccgact gaagcgtatc cttttctgtt tttgtagctg ttccagtttc
    28441 ttttttctgt ccgcaaccaa ccaggacaaa aaggaaagct agactaacaa gaactatttt
    28501 tttcatttct ctcttctttc tttttgaaat taaaatagaa taagactggg aagtgctccc
    28561 agccttgatg tttatagagc tgcacgtaaa cgtgcttctg cattttctac attacggaca
    28621 gagcgtggta ggaaggcacg aatatcgtct tccttgtagc caacttgcag gcgtttttca
    28681 tctacaagga ttgggctctt taaaattctc ggtgtttcca taatcagatt gagaacttca
    28741 ttgacactca aatcttcaat atccactcca agggctttgg catagcgatt tttagacgaa
    28801 acgatgctgg ctattccgtt atctgttttg gttagaatat ccagtaattc ttctctcgta
    28861 attccttctt taccaaggtt ttgttcttta taacttaact ggtgggcatt gagccaggtt
    28921 tttgcttttt tacagctagt acaacttgag actgtataaa ttttaatcat gtacctaccc
    28981 ctttcgctac atgttactat cagtttagtc tattatacca taaaaaacat ccgacttgcg
    29041 acctattttt aa
//
LOCUS       DAVKJU010000023        25478 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVKJU010000023 DAVKJU010000000
VERSION     DAVKJU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.023"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(77..625)
                     /locus_tag="VW191_000953"
     CDS             complement(77..625)
                     /locus_tag="VW191_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEU9623740.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(640..1488)
                     /locus_tag="VW191_000954"
     CDS             complement(640..1488)
                     /locus_tag="VW191_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEU9623741.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVCQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            1676..3025
                     /gene="trkA"
                     /locus_tag="VW191_000955"
     CDS             1676..3025
                     /gene="trkA"
                     /locus_tag="VW191_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEU9623742.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            3029..4468
                     /locus_tag="VW191_000956"
     CDS             3029..4468
                     /locus_tag="VW191_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9623743.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNQTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(4594..6273)
                     /locus_tag="VW191_000957"
     CDS             complement(4594..6273)
                     /locus_tag="VW191_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEU9623744.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEDVASSNDIILEEETSEIMPGELDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKSLQFVLEHYQAV"
     gene            complement(6343..7755)
                     /gene="lacG"
                     /locus_tag="VW191_000958"
     CDS             complement(6343..7755)
                     /gene="lacG"
                     /locus_tag="VW191_000958"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9623745.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHDLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSAFWVKGLKRN"
     gene            complement(7765..8109)
                     /locus_tag="VW191_000959"
     CDS             complement(7765..8109)
                     /locus_tag="VW191_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9623746.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(8277..10163)
                     /locus_tag="VW191_000960"
     CDS             complement(8277..10163)
                     /locus_tag="VW191_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEU9623747.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGHRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHIWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWVPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGQMLWKKTRSYEELIEEYFFALYGENWQSVIEYLEKL
                     SIYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(10165..11487)
                     /locus_tag="VW191_000961"
     CDS             complement(10165..11487)
                     /locus_tag="VW191_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9623748.1"
                     /translation="MNNILTFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VTAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPSIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11654..12877
                     /locus_tag="VW191_000962"
     CDS             11654..12877
                     /locus_tag="VW191_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9623749.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            14311..14595
                     /locus_tag="VW191_000963"
     CDS             14311..14595
                     /locus_tag="VW191_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623750.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            14842..16151
                     /locus_tag="VW191_000964"
                     /pseudo
     CDS             14842..16151
                     /locus_tag="VW191_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(16224..17075)
                     /gene="srtC-3"
                     /locus_tag="VW191_000965"
     CDS             complement(16224..17075)
                     /gene="srtC-3"
                     /locus_tag="VW191_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEU9623751.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(17062..17955)
                     /gene="srtC-2"
                     /locus_tag="VW191_000966"
     CDS             complement(17062..17955)
                     /gene="srtC-2"
                     /locus_tag="VW191_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEU9623752.1"
                     /translation="MDNSRRSRKKGTKKKKHPLILLLIFLVGFAVAIYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEDILQKGAGLLEGASLPVGGENTHTVITAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            complement(17942..18844)
                     /gene="srtC-1"
                     /locus_tag="VW191_000967"
     CDS             complement(17942..18844)
                     /gene="srtC-1"
                     /locus_tag="VW191_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEU9623753.1"
                     /translation="MVKTKKQKRNNLLLGVVFFIGMAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPVIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     KQPEKALKALKAARKEVKVEDGQQ"
     gene            complement(19182..20363)
                     /gene="rrgC"
                     /locus_tag="VW191_000968"
     CDS             complement(19182..20363)
                     /gene="rrgC"
                     /locus_tag="VW191_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEU9623754.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKPN
                     N"
     gene            complement(20410..22374)
                     /locus_tag="VW191_000969"
     CDS             complement(20410..22374)
                     /locus_tag="VW191_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEU9623755.1"
                     /translation="MKSINKFLTILAALLLTVSSLFSAATVFAAEQKTKTLTVHKLLM
                     TDQELDAWNSDAITTAGYDGSQNFEQFKQLQGVPQGVTEISGVAFELQSYTGPQGKEQ
                     ENLTNDAVWTAVNKGVTTETGVKFDTEVLQGTYRLVEVRKESTYVGPNGKVLTGMKAV
                     PALIILPLVNQNGVVENAHVYPKNSEDKPTATKTFDTAAGFVDPGEKGLAIGTKVPYI
                     VTTTIPKNSTLATAFWSDEMTEGLDYNGDVVVNYNGQPLDNSHYTLEAGHNGFILKLN
                     EKGLEAINGKDAEATITLKYTATLNALAVADVPEANDVTFHYGNNPGHGNTPKPNKPK
                     NGELTITKTWADAKDAPIAGVEVTFDLVNAQTGEVVKVPGHETGIVLNQTNNWTFTAT
                     GLDNNTEYKFVERTIKGYSADYQTITETGKIAVKNWKDENPEPINPEEPRVKTYGKKF
                     VKVDQKDERLKEAQFVVKNEQGKYLALKSAAQQAVNEKAAAEAKQALDAAIAAYTNAA
                     DKNAAQAVVDAAQKTYNDNYRAARFGYVEVERKEDALVLTSNTDGQFQISGLAAGSYT
                     LEETKAPEGFAKLGDVKFEVGAGSWNQGDFNYLKDVQKNDATKVVNKKITIPQTGGIG
                     TIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            complement(22598..25258)
                     /gene="rrgA"
                     /locus_tag="VW191_000970"
     CDS             complement(22598..25258)
                     /gene="rrgA"
                     /locus_tag="VW191_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEU9623756.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTDGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQWTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYEQKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNAFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAAGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVLYDTTEKRIRVTGLYLGTDEKVTLTYNVRLNDEFVSN
                     KFYDTNGRTTLHPKEVEQNTVRDFPIPKIRDVRKYPEITISKEKKLGDIEFIKVNKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLPFYLIGCMMMGGVLLYTRKHP"
BASE COUNT         7872 a         5571 c         4106 g         7929 t
ORIGIN      
        1 tatccacaat ctcaaaacac tgttttgagc aacctgcggc taacttccta gtttgctctt
       61 tgattttcat tgagtattag tcctttttca aacttcctgc acgagtttgg gttcctgcat
      121 aggcaagtaa gagaagagtt cctgcaatag ctacagatac accattggca attcccgcaa
      181 caatcccttg tgcaaatact ttttctgccg cttcttgata aatcacaaca tctccaagtg
      241 gtgccaagac accccaaaca agggcatttg caagtagttg aatgaggtta aaaataagaa
      301 tatctttcca gtcaaaaaca ccattgatca ctcgaacgta ctttctaaaa agtcccacaa
      361 ctagaccaaa gagtccgcta gcgataatcc aagtccacca tagaccataa ccagcaagag
      421 agtccttgat tgcatgacca atcaacccga caagcaaacc gataatcggt ccaaaaataa
      481 tagaaagtag cgcttgtacc gcatactgaa gctggatgct tgtatttgga acaggggttg
      541 gaatgttgat catcccgatg acgacaaaga gggcagcgcc aattccgaca gcaacaactt
      601 gtttaattgt aaatttgatt tccatactat tctcctattt tatccttcta ttttctttat
      661 ttcaatggtc caagatgaac cgacacctac attataggcc ttggcaaagg aaccttggtt
      721 gatagccaaa cctaaacgat agagagagtt gatgtaaagg atgggttgcc caattctcac
      781 atctgcaaat gatttgccat agacaacctg attttgacag accagcatat cagcatgata
      841 gatggtcact tcaaaacgat caccaaattc tggttccagc ttgtaaaatt cttcccgtgt
      901 gatagaggtc caaagcgaac cgaaacgcac atccagaata tcaatggctc ccttcaccag
      961 atgatcttct atgatggtcg ctacgactgg aagctctaca atctgttcca cactgagctc
     1021 tggccctact tcctcaaaag taatgtgacc actggccagt ttagcaccag tataggcata
     1081 gacatcacga ccgtggaagg tataagaatg ctctgtgttt tgacgcctat tggccacctc
     1141 agaaatctca cgaatggcta caatgccaac gtgtttcttg ataaaggaaa gcgtcccatt
     1201 atctggcgtg acaatgtatt gattttttgc agtcttggca actacactct tacgtttcga
     1261 accgacacct ggatcgacaa ccgatacaaa cgtcgttccc tcaggccagt aatccaccgt
     1321 ctgaaagaga cgatagctcc cctcaaaaat attataaggc gtgatatcgt gcgtcaagtg
     1381 atggattttt aaggttggag actcttctaa agccactcca atcatagccg ataccgcacc
     1441 atcaaccaga ccaaagtctg attgtaatac cagtaaatta ttattcattt tatctccttg
     1501 tatatccttt atcataccat atttcagaga aagtcgctaa tagctatttc aattggttag
     1561 ggtatagttt taccttatag cgaattttca ctaaaagctc ttgttattag ctagtaggtg
     1621 cattttttct tgaaaaaggg tatgatagtt acatcatgaa aaagtaggtt ttattatgaa
     1681 aattatcctt gtcggagggg gaaaagttgg ttttgccctc tgtcgctcct tggttgcaga
     1741 aaagcatgat gttttgctga ttgagcaaga cgaagctgtt ctcaatcata ttgtcagtcg
     1801 ctttgatatc attggtatcc ttggtaacgg ggccgatttt gccattcttg agcaagccag
     1861 cgtccaagat tgtgatatct ttatcgccct gactgagcac gatgaagtca acatgattgc
     1921 agcagttcta gccaagaaaa tgggagctaa agaaactatc gttcgggtgc ggaaccctga
     1981 atattctaac tcttatttca aggaaaagaa tattctcggt ttttctctta tcgttaatcc
     2041 tgagctcttg gctgcccgcg ctatcgcgaa tatcattgac ttccccaacg ccctgtctgt
     2101 cgaacgcttt gctggtggac gcgttagcct catggaattt gtcgtcaagt ccaccagcgg
     2161 tctttgccaa atgcccattt ctgattttcg taaaaaattt ggtaatgtca ttgtctgtgc
     2221 gatagagagg gatcatcaaa ttatcattcc aagcggtgac atgactgtac aggataaaga
     2281 tagaatcttt gtcactggta accgtgtcga tatgatactc ttccataatt attttaaatc
     2341 acgcgccgtg aagagccttc tcatcgttgg ggcaggtaga attgcctatt atctacttgg
     2401 tattctcaaa gatagtcgta tcgatacaaa agtcattgaa atcaatcctg aaatcgccag
     2461 cttctttagc gagaaattcc caaatctcta catcgttcaa ggagatggaa ccgcaaaaga
     2521 tatcctgctg gaagaaagtg ctcaacacta tgatgccgtt gcgactctaa caggagtcga
     2581 tgaggaaaat ctgattacat ctatgttcct tgacagggta ggtgtacaga aaaatattac
     2641 taaggtcaat cgtaccagtc tcctcgagat tatcaatgcg cctgattttt caagtatcat
     2701 cacacctaaa agcatcgctg tagatacgat tatgcacttt attcgtggtc gagttaatgc
     2761 ccagtattca gaccttcaag ccatgcacca tctagccaat ggccaaatcg aaaccctgca
     2821 attccatatc aaggaagcca ataaaatgac tgccaaacct ctttctcaac tgaaattgaa
     2881 aaaaggggtt cttattgcag ccattattcg aaagggcaag actattttcc caactgggga
     2941 ggatatgttg gaagttggag acaagctcct agtaacaacc ttgttgccaa acatcaccaa
     3001 aatttatgac ttgattgcga ggtaagaaat gaataaaagt atgattcgtt acctcctttc
     3061 aaagttactt ttgattgaag ctgttctcct cttggttcct gtggctatcg ctgtctatta
     3121 ccgtgaatcg agccaagtct ttacagccct cttttcgacc atagggattc tcgtattgtt
     3181 aggcggttca gggagtttac agaagccaaa aaatcaacgg atttatgcca aggagggagt
     3241 cttgatcgtt gccctctgtt ggatcctttg gtctttcttt ggtggtctcc cctttgtctt
     3301 ttctgggcaa atccccagcc ttattgatgc cttttttgaa attagttctg ggtttacaac
     3361 tactggagca agtattttga acgacgtttc ggttctcagc cgttccctcc tcttctggcg
     3421 aagttttacc cacttgattg gagggatggg agtgcttgtt tttgcacttg ctattatgga
     3481 caatgccaaa aatagccacc tagaggtgat gaaggctgag gttccaggtc ctgtttttgg
     3541 caaggttgta tccaaactaa aaaacactgc ccagattctc tatctccttt atctagctct
     3601 cttctccctc tttgtcataa tctattatct agctggtatg cctctctacg atagttttgt
     3661 cattgctatg gggacagcgg gaactggagg ctttaccgtc tataacgacg gaattgccca
     3721 ctatggcagc tcactgatta cctatctggt cagtatcgga gttctggttt ttggggtaaa
     3781 tttcaacctc tactactacc tcatgctccg tcgcatcaag gccttctttg gtgacgaaga
     3841 acttcgggct tacttggtca ttgtactggt ttctacaggc ttgattagcc tcaacaccct
     3901 ctacctctac ccagggtttt caaagagctt tgaaatgacc ttcttccagg tttccaacat
     3961 cattacaaca actggttttg gatatggaga tattaccaac tggcccctct tctcccagtt
     4021 tatcctcctt ttcctcatgg caatcggtgg ctctgctgga tcaactgcag gtggactcaa
     4081 gattattcga ggcctcatcc tttcaaaaat tgccaaaaac caaattttgt caatcctatc
     4141 gccccaccgt gttttgaccc tccatgtgaa tcaaacggtg attgataaag atacccagca
     4201 taagattctc aagtactttg tcatctatgc tatgattttg ctagcgctta tctttattgt
     4261 cagcctagat agcaatgatt ttctggtcgt gaccagcgct gtctttagct gtttcaataa
     4321 tatcgggcct attctaggaa ccacttctag tttctcaatc tttagtccca tctcaaaaat
     4381 tctcctatcc tttgcaatga ttgcagggcg cttggagatt tacccaatcc tccttctctt
     4441 tatgaagaga acttggtcta agagataaaa tacaaaatcc ctctttttac aattgtgaaa
     4501 gagggattct tatattaaaa aagggggaac atttttaaat ccaagcctat ctgtaagcaa
     4561 aatccttact caaataaaag atggaaaaac ttactacaca gcttggtaat gctccaatac
     4621 aaattgtaag gatttacttg gactctttgt taaatgaata tattccattc ctctggtagc
     4681 aactatctga atacctaatc tttctgcatc caccttcatc tctctataat aactccgaac
     4741 ttgtggagcc agaataatta aatcataaac acccataata tcataatgag ctccgtacgc
     4801 tcctgaattc gcaatcactc taacttctgc taattgagcg ccctcgttaa ttgcattggc
     4861 taattgcgca cttgttccag accctgcaca aagaaccagt actttcaact ctttactttt
     4921 tatttcatct aactcaccag gcattatttc acttgtctcc tcctctaaaa taatatcatt
     4981 tgagcttgca acatcttctt tcatcagtaa ctgtctatca tacgctctac agaatggcaa
     5041 ataaatcaat atgtcgacaa ctaaaatcaa agataaaaat acaaaggaga tagattgaaa
     5101 attcgttcca attaacaatc ccaagggacc aggaaaggcc caaggtaact ggatataaaa
     5161 tccattcatt ccaaagaaat caataaagac ctttcctaga aatacattca ctggtggagt
     5221 catcaaaaaa ggtacaaaaa gatagggatt caaaataact ggcataccaa atagaagagg
     5281 ttcattcacc gcaaataaaa caggagtaat tgtagcttta cctaccgatt ttaattgttt
     5341 agaccgcata aagaaaatca aaataaatgg tacaacaaag gtagcccccg ttcctccgat
     5401 agcagcgata taatttccga aattaggcgt taaagaatga taaggaaact gcccatttgc
     5461 aagaagttga gcattctctt ccatattgct cagttgtaaa gctgtaacag caggtaagac
     5521 aatagatggt ccatgaattc caacaaacca aagcaaagcg cacataaacc aaatcaacat
     5581 catagcagga tatgattctg cccccttaaa aataggagtc aatagttgtt gaaatacttg
     5641 ggcaaaagga acatctaaac taaaccgaga tacaatatct aacaaaccac taataagtaa
     5701 aacaaaagaa aaaggaaaaa tatctctaaa agcttgtgat atagccccag gaacttcctt
     5761 aggtaaatga atagtaatgt ctcgcttaat acagaatcgg taaacattta cagtaataaa
     5821 ggcactgaca aacgaagtta tcaatccttg agatcctaaa tagtttgtcg acaaacttgt
     5881 agatcccgtt ttctcatcaa ctacaagcgt tacagttaat acaagatagc cacatatggc
     5941 cgccaacatt gcagaagtat catttattac ctttccagga ggcatttttc tattaacatt
     6001 tccagccaat gactttgcaa ctgtccctga aacaataaca cccaaaactc ccatggttag
     6061 catatacact ttcctcatcc aggcaatagc tggactcggt aactctactc cgaaattttt
     6121 aggaatcata ataataatca tcaaaaagct tgaaaacata attaaaggca tagtagcaat
     6181 aaatccatct ttaatagcca tcatataatt attagtagaa attttttcaa atttcccttg
     6241 ttgcttttca atggcagcga ctaacttatc cataaaaact cctcctgtaa aaaatttaag
     6301 ctcatagtta aaatatatta aacatcagtc aaatcgctaa ccttaattcc gttttaatcc
     6361 ttttacccaa aaagcacttt tcttaatata tctcttctgt gtttcataat caacaaagaa
     6421 aagaccatat cgcttattgt agccattcgc ccaagaaaat tgatcttgca aagaccatat
     6481 aaaatagcct tgaatattga ctcccctatc tcgagcctct aaaactttgt gtaaatgttg
     6541 ctcaatataa cggattcttt tactgtcatc aataatatca ttctcgccct ctggcttaac
     6601 ctctttaagg gctgtaccat tttcagttaa ataaatgacc ggatgttgag gatattcttc
     6661 tttgatacga agcaacatat caaataaccc ttgaggataa atattccaat cccaatctgt
     6721 tgtcggaata ccaggctttt ttacaaattc acctagagca tttaatttaa aagaagactg
     6781 tccttttatt cctgttgaat taaattcttg tctattttca cctttgtatt cacgaataaa
     6841 ttctgaacga taataattca tcccaaacat cgtattaaga ggtgcagctc ttttcataat
     6901 ttctaaatca ccatcttcaa taataaagct agaattatta gcttccaata tttgattcaa
     6961 gtgaaaaagc gtgtcatcac tgtagtagcc caaaaaagtt ccatctaata gaaatttatt
     7021 attataaaca tcataccgtt tagctgctaa aatattttct ttttgcccat caataggata
     7081 gcctggcttt aaagcatgaa tacaacctat ctctccctca attcctaatt gatgaaattc
     7141 gaggactgca agagcatgcg ccaacaacat attatgattc gcttgaattg cttcagataa
     7201 ttgaaaatga tgatttggag gaaactgacc tcctatatat tgacctgcag caagagacat
     7261 cagttcattg attgtaaacc aatgcttgac ttctgtaaat tcttggaaac aaaagcgagc
     7321 atatcgtatg aaacgatcaa tattctctct attcaaccaa tcccctgttt ctaacatttt
     7381 ctgaggcgaa tcaaaatgat gtaaagaaac aaacggaatc acattatgtt ttaagcaaga
     7441 ctgaaagact ctatggtaat aacgaacagc taatatatta ggctccccat ctatatcagg
     7501 aaatatacga acccaagaaa tagataaacg caaagcctgc aaatcatgtt ctgccgccaa
     7561 agctatatcc tcttcgtaac gataataaaa atcactagct ggatctggta agaacggact
     7621 gttttcttgc aaataaacat cccacatatt tgttcctttg ccatctaccc tagttgcacc
     7681 ctctacttga taagcagcgg tagcagctcc taataaaaac tccttcggta aggctttaac
     7741 ctgtaaattt tccatgacta atcctcattg caaaccaata tgttcttcaa tctttcgaat
     7801 tcgttcatat tcatcaataa aaaacttgac ctgatcatac aatagcatcg ttgtcatcaa
     7861 ggtatcttga ccatgaatca tgataaaact cacttcaaaa ttatctccag atgcctcctc
     7921 cgctaataaa ttagtctgtt ctcgatgggc agacactatt gagtcgtttg cagactcaac
     7981 caattctctt gcctgttcaa aatatccatc tctcgcaaac gccaaagcat ccatcaaatc
     8041 tgaccttgca tctcctgcgt aagcgacaat tgtaaaacca agcatattta tttcttcttt
     8101 tgatttcatc tttcttcctc ctcaatcttg atccatcttt tacacatcat tattaaaatg
     8161 acttcgctac gatataaata gaaagtcgac cctaaatatc ttaaaaatcc ttaaatttca
     8221 taatatttag gatcaatctt ctctgaatac cttggttcat tttctatact tatgatttat
     8281 aagtggaaac cagcgtaatt ttttgctact tcaattacac gataaacatc caaattagat
     8341 tgaaatagca attcgtgccc acggatataa ttcaacacat ttctccattc atcttgagct
     8401 tgccttgttt ttccagttgc ttgaagatat aaagccttcg ccatcttaac aacatattca
     8461 cgatgataac tgagctgttt ccattcatcc ttttgactat ttaataactt agaaatattt
     8521 tcctcaataa ttggtaaaaa attatcagct agattgtaag ctatataata atgattcgct
     8581 aaaacatcat tttgacgact gccaattgca ttaaaatagt cacaagagga ataaatggat
     8641 aatttttcta aatattcaat aacagactgc caattttccc catacaaagc aaaaaagtat
     8701 tcttcaatca attcttcata acttcttgtc ttcttccaga gcatttgccc catgacataa
     8761 ttaggaaaat tatgagggaa tccggcacgt aattcttgac acgaaatgta cccattcaaa
     8821 tgtaggtttg aaagataaga tacatctctg taaatagttt gactaatttt catatagcct
     8881 aaatcgccat aatgagcacg ccctaaagga tagtcatata cgaaactatc tcctttaaat
     8941 gttttttgcc actcaaaaag ataagataaa ttttcctcaa gagaattcgg aagtatgatt
     9001 ttattacgca gataaggttt aggcgtaggt atggaattgt caaaatctac atctgcataa
     9061 ctcatttcaa atgttcttgt aatcggtgca aacatcatgg taaagcgttc aggattatct
     9121 aatttttctt tctgaggcac ccataacaac tcatggtaaa gcagaaaaca aatctttgta
     9181 tctaatccct cactcgttaa agccctatcc aattgattga gaatacgaat atactgatca
     9241 gaaaccaatt cttgtctaca gttttcgcat tcacaaatat tattacgagc atccgacaac
     9301 catatatgta agtagttaac atcaggtctt ttcttggcat aatccttgat aatttctacc
     9361 atcttatcag ctacatctgg atttgaaaaa tccaggctgg ttaaaatcgg agccgtatta
     9421 aacaattctc gtttcccgtt tatttcagcg acatagggtt tcttctcctc tgaaatacta
     9481 agacctgatt cccagccaaa ttttgaagag taacctaaaa cttcacctgt ccatccatga
     9541 ccaacacgat gatgaataag acctcttttt tgcaattctt tatccaacct atcactcaat
     9601 tcttgtacta attcatttga aaattgttct ttatttagat atggattaaa ttcatgttca
     9661 taccaacgtt tcaaaaaaga gtaaggattt tcaaactgga tgaaaaaact gttcatccca
     9721 atcttaggta gccaatcaat gaaatctagt atattttcaa atgaatccgc tccctctata
     9781 catactcccc tatgatagta actggctgtt tcatctatag atagctgttt atctaaaaaa
     9841 tcttcaaatc gtaactctgg aacaaagtca tgtctgtgcc ctggtctagt atacacaacc
     9901 ccaaattcat gaaacattcg gtaaattcca ataagtaaac ctacaattgt attagactca
     9961 attctcccct tacccttgtc taatgagata agaacatggt cttttctatc ctctttattg
    10021 actaatccta caaaaatatc cgcttccttc tcctcgacaa gctctatgtt agtcttaaag
    10081 acccgattca tatagtaact taattcctct aaggcaaagc tagaataagt atttttctgt
    10141 tgatcaaata ctctgataac catcttattt ccctccaaca gtagcttttt ctttttcaag
    10201 attgcgttta tctgcaattt tgaagaatgg ataataaatt gctacagaaa ccaaaatcaa
    10261 aacaacttgt aatagagctc ctcgccaact agctcctgtc gcaaggaagc ctcctatgat
    10321 cggtggcatt gtccacggaa ggattacacc tgttgtatag ggtactaatc ctacagccat
    10381 tgatacatag gtaatcaagg cattgattgt aggagtagca atagacggaa tcagcataat
    10441 cggatttaaa actgttggaa acgtaaatag aatagctgta ttgatattaa aaatagacgg
    10501 tataatagct agcttaccta atgttttatt cgcacgactc ttactaaata ggaagagaca
    10561 aatcgcaaga ccaatagtcg ctccaccgcc accaataaat acaaataaat ctttaaacgg
    10621 taatgtaata atgtgttgga gtgtttgtcc tgcagtcaca gcttcttggt tttctgtagt
    10681 aaattgtaac caaactgggt ctacaaaagc atttaaaact tgtcccccat taactccaca
    10741 gaaccaaaag aatgaattta caataacaca tagaatcata cctggaagcg ttcctgcaat
    10801 taacttaagc ggtgttccaa caattgcacc taggagtccg ttgagacctc ctgcaactcc
    10861 tgccgcttca agacctttta agaccagagc ccacaaaaca aaagtagtaa agccaggtaa
    10921 aagagctgaa aatgatttac ttaccacatc tgggacacca cttggcattt ttatcgtaat
    10981 tccgcgctgg ataaacatac gataaatctc tgctgtaacc aatccaatag taatcgccat
    11041 aaacaaagaa gatgcattca ggctagaaaa tggtattgcg ccgccaaata actgcttgac
    11101 ctgctcccca tttttatcat aaaccatact cgaaaaacga ggtgccatca atacaaaact
    11161 ggataaggct atgatccctg ccgacggacc atctgtacca tatccttccg acaacctgta
    11221 ggcaataccg aaacaagcga ccaaggagat aatcgccact gttgactgat tcatagttgt
    11281 caagatactt agcaatccaa cactattcag ccaatttgta aaatcctctt gaggccaact
    11341 gattagaata agaaataaag agccgactaa aattaaaggc attgccatca taaatccttc
    11401 acgaatagct ttcaaatgcc gttggttgcc aactttttca ccaaacggag cgacttttgt
    11461 ttctaaaaac gttaaaatat tgttcatcac cggaactcct ttttttattt tttataaatt
    11521 aaatatacca ctaatagaaa gcgttgtcaa tatatttttt gctgttttta caatattttt
    11581 tctttgtata gaataataat tttaattgat tttttcttat gtttaatata aaataaacca
    11641 aaggaggaaa caaatgatta caaaacaatc agccttttta caaaatagag ccactatact
    11701 ggagtttcta tatcgaaatc cagcaacatc tcgtacagat attgttaatg aaacaggact
    11761 tactccagca acaacgacta acatcataaa agagctaagc gaacagtccc tcatatacga
    11821 aactggtgat gaatttagcg agttttccgg atctggaaga cgtagaaaaa ctatttctat
    11881 cacagataat attccgtatg ttgttggagg tattgaaata aacgtcctag gtatctttct
    11941 cagtctatgt gacttacaag ggaaaactct tttcgagaca gaaattttga atgaagatta
    12001 tcctatttca gaaatcaatt ccaccattac caatatgata aaaacagcta tagagtacgt
    12061 ccctttggaa acaaaattac ttggatttgg cttatcaata cctggacatt ataacaaaga
    12121 ctccggaagt atcattacaa acaaccccat atgggaatct tttaatttat taaatgtaat
    12181 taaaagattc aattttcctt ttattgtaaa aaataatatc gattgtatgg ctataggaca
    12241 ataccttttt aatccacaca atacccccga taactttatt ttcctacacg ctggattagg
    12301 tatttacact tcctttttca caaaagaaaa aataggagcc tctaaaaatc cttatatcgg
    12361 agaaattgga cacaccattg tcgaattgaa tgggcaatat tgtgaatgcg gaaaaaaagg
    12421 ttgtttacaa acatatattt cggatgcttg gttaatcaaa cacgcccaat tattatttaa
    12481 aaattcccaa ctaactgtac taaaaagcct tgtaaagact gaaaaagaca ttcatttaga
    12541 caccctttta acggcttata atttaggcga ctccgcttta cgtcaacaaa ttgataaagg
    12601 agtcaattta ttagccactt ctattgcaaa tctcctcctc atcaatcctg ctgataaaat
    12661 ctatatcaac agtcaattgc ttaattatca acctttcact catgaagtca gggataaaat
    12721 ccaagaccag ctccacttcg ttccctttac tcgtaatata gaaattgaaa ttttacctta
    12781 caacaaacat cgtggaagta taggagcttg tgcattagct atcgtcgctt ttttcataga
    12841 acatagcaat gtattacaag atattatttc accttaatat attagaaatc tatagacctg
    12901 tttaaatcaa ctataacctg tagtagatat ctcgtattta gacaatatga aaacaagacg
    12961 acttccatat aggaaaccgc cttctcgcta tgttgagtga tttatattaa aataactttt
    13021 cttctagctg cattttatta ttataaaaac attcatcata acccccagaa cttaaataac
    13081 aatttttatt caagatacat actcctagaa taaactttat atgaaattct catttttgtt
    13141 tttacaattc tccttagtta aatcttgttt aatatatgtt ttacatatag tatttagcgc
    13201 cacatagtac tgaactctct ccaaaaacgg ttattcctct ttgaataggg cgttatcaca
    13261 agaaaagcat ctccacgttt caacttcata tggctcaaaa acaatcaatt gatgctaaaa
    13321 cctgtaccta gatgtttcgg ttcataaaac catgaaactg taaaagtgga tgaaattgat
    13381 agcgatagtc aaatcaagag gcatcataac tctaaaaagt cacaatatat aagttcatcc
    13441 tcggaaaaat atcattctaa ttgttgaaat gcctacatga aaagaaacgt caaatgctca
    13501 tgaaacaacg aatacaggta tcaaaactat gacaaaacaa atccctaaat ttactaaaga
    13561 cactgctcaa ctttacacct gtaaatggtt gttgtataat aaagttacaa agatgtacga
    13621 ccacactgtt gtaaatcata gtgttcgcga atatattact gatagcattt ctacaaatac
    13681 aagtaaagag agcggatgag attcaaacga aatatgtcag tgctttggca ttcctagcct
    13741 tcatatcatt taaagaattc tatagacaaa attttttcca atacagacac tcgtaacaac
    13801 tgcttcattt ttctaccaac atatttagga acaggataag atacaagagt attaatccat
    13861 agctcagttc tataccaatc taagacaaat aagctaaaaa aacgattgat aataagcaaa
    13921 tagattccaa attttctcta tctgctcatt ttaataaaca atactagtgt aactatcctt
    13981 ccagtcagaa acttgtcaaa tcacaccgaa aactcttcta aaatttatct cgttaggcaa
    14041 tcaagcaaaa actcgacgat agtacaaaca ttatcataca gaattgactg cctaaattat
    14101 atactttagt aaggttttcg gataagaaaa aaggttcatt ttacatttct aaacattctt
    14161 ttctaagatg aaaaacagaa tttttcgatt gtgatttaaa gcaacaagaa gattttcagt
    14221 atcatcctat agatacgagc taattaagaa aaactacatt tttgaatata aactacaata
    14281 atataaacta aattttatag gaggaagaca atggattggt acgattatat gatacaggca
    14341 tccaaacaat cacaattcaa cgcaagccat tggtttcgct atttgcgaaa agttattttt
    14401 gaagactatt cttatttaac aaaccaagat gtagaaaagt tgctagactc caaagaacta
    14461 acccgttttc aaaaaattag cttgaagtat gcctttcaag agcatactcc aactcataaa
    14521 tatgtgattt cattaaataa acctgctaag ttaaccaatg ttcaaaaatt gatggagaaa
    14581 tacaaacatg gataaaatga aaccggtctt ccaagcccta aataagaaat taattcagga
    14641 aaatctgact ttaacaatta tggttatgtc ttagaatatc atggtttacg tgccacacaa
    14701 gatgttgatg cttttatggc tctataatat ttgtagtggg taaatcccct atggatatta
    14761 tggagcctat ttttgtgtag aaaaaaagtc ccatatgacc tataatgaaa agcgacaaaa
    14821 caactcatta gaaagaatca tatggaacaa ttacatttta tcacaaaatt actagacatt
    14881 aaagacccta atatccagat tttagacatc atcaataagg atacacacaa ggaaatcatc
    14941 gccaaactgg actacgacgc cccatcttgc cctgagtgcg gaaaccaatt gaagaaatat
    15001 gactttcaaa aaccgtctaa gatcccttac ctcgaaacaa ctggtatgcc ttctagaatt
    15061 ctccttagaa aacgccgttt caagtgctat cactgttcaa aaatgatggt cgctgaaact
    15121 tctatcgtca agaagaatca tcaaattcct cgtattatca accaaaaaat tgcgcaaaag
    15181 ttgattgaga agatttctat gaccgatatt gctcatcagc tggccatttc aacttcaact
    15241 gtcattcgca agctcaatga ttctcacttt gagcatgatt tttcgcgtct tcctgagatt
    15301 atgtcctggg acgttgaaac agtccgggga gtgactgttt caatcgggag atggagatga
    15361 gctttattgc gcaagatttt aacaatctca atatcatcac tgttcttgaa ggtagaacac
    15421 aagctgtcat ccgagatcac tttcttaaat atgatagagc cgtccgatgt cgcgtcaaaa
    15481 tcattactat ggatatgttt agcccttact atgacttggc taaacagctt cgctttcaaa
    15541 tttctaggct caggctgaaa cagtctccta atgctaaaat cgttcttgac tgctttcata
    15601 ttgtacaaca tcttagccgt gctatgagtc gtgtgcgtgt ccaaatcatg aatcagtttc
    15661 atcgaaaatc ccatgaatac aaggctatca agcgctactg gaaactcatt caacaggata
    15721 gctgtaaact gagtgataag cgattttatc gtcctatttt tcgtatgcac ttaaccaata
    15781 aagagatttt aaacaagctt ttgagctatt cggaagactt gaaacaccac tatcaactct
    15841 atcagctctt gctttttcac ttccagaata aggaaccaga gaaatttttc ggacttattg
    15901 aggacaatct taagcaggtt catcctattt ttcagactgt ctttaaaacc ttcctcaaag
    15961 ataaagaaaa gattatcaac gcccttcaac tacactattc taatgccaaa ctggaagcga
    16021 ccaataatct catcaaactt atcaagcgca atgcctttgg ttttcgaaac tttgaaaact
    16081 tcaaaaaacg gatttttatc gctctgaata tcaaaaaaga aaggacaaaa tttgtccttt
    16141 ctcgagctta gctttttttc aacccactac agttgacaaa gagccggaaa aaggaacagc
    16201 cttagctttc ctttcatttc tttttatttc cctcgtagta aacgtgctag cttccacaaa
    16261 acaaacagga ttcccagaaa tgccagtacc actagcccac ggtacaacca ttgagaggtt
    16321 gcaacacgcg atacagattg tccttctttc gtaaaagcaa ccctcgcaac tgcagctgtt
    16381 tgtggatctg atttttgata aacagcgact cgttcaaaat tcactaataa gcgtttatta
    16441 aaggtaggaa tcggatcgca ggttatcaag gtcatgatat ttttagagct aaccgattct
    16501 aatttttccc attccgacgg taaaataatc tctgtgtcca tcatctgata ttctacaatt
    16561 tcctggccat tatcataata aagagcatct ccaactttta gctgatccaa atggcggaaa
    16621 aagacatggc ttggctctgc acggtgccca gcaatcactg agcgaatccc tgtaccatcc
    16681 agaggcagcg gtgtaccatc cacatgagcc aagcccatcc ctaaatgatg ataatctgct
    16741 cccaaataaa ccggctccat gatttccaaa cttggaatag acaagtaacc atagactgca
    16801 tcagggtcgt cagacacttg gtaattgacc tcatatccct ccgccaaaaa aggatctaca
    16861 atgcgatttt gcgaagccaa gcgttgattg taggcgagag aatggttctg ttgttcttgg
    16921 tacatttcag ttgtcatgga tttcacaaat gtagcatgac ctttcacctg tccaagagac
    16981 tgcaacacca tctgtccaaa acaataaata ggaatcaaac aggctaccaa catcaacaag
    17041 tatcccaata aggctcgtag tttagtcctt gacatgacgc ccctccaatt gcttttctag
    17101 tcctttgaca atccgtcgat tacgatacac gcgatacagc aagagaagga tgaccgccat
    17161 cgctcctagt aataaccaca accagaattg cccacgctct ctcaccgctc gattccgctc
    17221 tgcaattggt gccgtatacg gaatccgctt cccacgtacc aacagacgat gactgttaat
    17281 catatacggt gtacaagtca acaaggtcgc ataatcttcc ccatgttgaa tcaagacagg
    17341 ctcaaagtca ttcggctcca ccgtcactat ctgatccact tggtaggcca acacctgatc
    17401 taaaacgtga agataaaaga tatccccttt tttcatctta tccaattgac tgaacaattc
    17461 tgccgttggc aatcctctgt gagcagtgat cactgtatgg gtattttcac ctccaacagg
    17521 cagcgaagcc ccttctaaca gccctgcccc tttctgaaga atgtcctcac tcgttccgac
    17581 atacatcgga atttcctgat caatcgcagg aatttccaca tagccaatcc gctcatggac
    17641 ctttagcata ttggcatatt ctgagacgcc tttctttttc tcttgctctg taaaaggatc
    17701 aagaatttca gatggtttca aggtcgcatt gaaggcttga gccaagcgcc aacgctcctc
    17761 aagttctgcc ttatccatct gggaaaccgt ctcatcaaac tctttaataa cctcgtttga
    17821 ctcaatacga taataataac gagacaccaa tggatatatc gcaacggcga atcctactaa
    17881 gaaaatcaga agaaggatca gcggatgttt cttctttttt gtgccttttt ttcgtgaacg
    17941 tctactgttg tccatcctcc accttcactt ccttccttgc tgctttcagc gccttcaaag
    18001 ccttttccgg ttgttttttc ttcttgcgca agcgtcgaat aatccataaa agaatcacaa
    18061 tcaaaccaac tgccacataa aacaggtagc gatagagatg actgagtttg tttgctgcaa
    18121 taaattcttc ctcaacctct gctacgtacg gtatccgatg cccccgaacc aatagacgat
    18181 gggtattgat catgtatggc gtacaagtca gcaaggtcac ataatcatga cctggtacaa
    18241 tcaataaatc atcaaagttc gtcggctcaa tcacctttac ttgatccact tgataggcca
    18301 tcacttcctt gatattgtgc acataaaact tatccccaac tttaagtttg gtcaaatccg
    18361 taaacatctt agctgttggc aaacctgtat gtgccgtaat caccgcatgg gtcgaattgc
    18421 ctccgatcgg cagagaagtt ccctctagat gcccagcccc ttgctgcaat acctcttcag
    18481 cagtaccagc ataaaccggc aaatccacgt caataacggg gatttccaca tgccccatcc
    18541 gctcatggat ttctaacata cgtgcatact ctgctcgccc ttttttcttc atttcttccg
    18601 accaaggatc gccactcact acattattca aagagtcatt gaaggcttgt gccaatttca
    18661 ttcgttcatc aatgtcagcc tcatccaacg ttgctttttc cttatcaaag tcagcaattt
    18721 gttgatttga ttccactcga taatacaagc gagacaccag cggatacgcc attaccgcca
    18781 ttccaatgaa aaataccact cctaatagga gattatttcg tttttgcttt tttgttttta
    18841 ccatttttat cagcatccct ttatcttcaa actcatcatt ttatcgcttt tcctaacaga
    18901 caatcctatc tgctaagaga aacgagaaaa tctcatttct ttctgattca ccatccattt
    18961 taaatcagaa tcccagtcct gtccattcct aaaatgatta ccagaccagt cctatcgttt
    19021 tacaaacaat aacccacaat gaggctggca caatctgccc agcctcatat ccgttattgt
    19081 gattcatgcc acatcaccat gattcctgat taacatcacc tcagagtact ctggtcttcc
    19141 cttcagcctg ctatcttttt cataatgatg tacattgaat atcagttatt tggttttttc
    19201 gtaagataat aaccactacc aaacaacaaa atggcaacaa gcatcaagat atacaaggtt
    19261 tcttcccctg tatctggcac atcaatccgt ggtcgcttgt tatttttaac cactgttacc
    19321 agttccttac gagtatcttt cccgattgta aaggaaacag gcgatgttaa ttgaacataa
    19381 ccagttggag cttggagctc ccataaatag tatgtcccat actctagacc ttccactcgg
    19441 aaacgaccat ctttccctga tgccacaact acttccttac cattttgaag aacaggagta
    19501 tagtgtccgt tttcttcttt catgactttg aacattgccc cttgaagaga ggtattggtc
    19561 ctaccatcca ccttcataaa gtcaacattg ccacgtggta atttctgatt gacaaccgta
    19621 atcgtcacca gctgatgatc taccaactgg acatccgtat ccatcgtcgt aacagtatag
    19681 cctgccagtg gctccacctc cttgaaacga taggtcccaa gaggaagatt tgtcacaaca
    19741 atctctccat ttttatcagt atagagagtt ctacctactt gaccagaaga actgtaacgg
    19801 tattctccaa tcaagggaac ctctttttca gaaccatctc ttgctactga taccaattta
    19861 aagccgacac cctccaagcg attgtggtct tgatccacct ttatcagctt cacctttgtt
    19921 gtcaccgtat ctgctttttt cgctacaatg accaaaggct ctaccgtctg atctgtcatt
    19981 tcaaaaagaa attcagctgg ataagaaacc gcatccgtct ggataataga gcgaacatag
    20041 taaagaccat ttggaatatg agatacttca atctgattct caaggaaggt catctcaaac
    20101 gaagtctttt tgaaagaaga aagtttattc tcatcccacg aatgcaagtc tctcacaatt
    20161 tgcacccgat tatcatagga atacgaatca tccaacttcc atacctgcaa ccgatgacca
    20221 tcacgagatg gcaattgact aaccacctcc tgatagttct ccaattgcaa gaccaacgtg
    20281 tgatcttctt gcgcttggac tgcatgtgca ccccatacaa gagaaaaaca cagagccata
    20341 acaaagaaga tacgactaat cattttctgc attttctgca ttgtcatcaa tggctccttt
    20401 ctctcttact taagcaagtt gatcctcatc tttgttgttt ttaacatatg cgtacactgc
    20461 aatacccata atcacagccc ccgctacagc aaagataatt gtaccaatac cacccgtttg
    20521 agggatcgtg atttttttgt tgactacttt tgtagcgtcg ttcttctgaa catcttttaa
    20581 ataattgaaa tcaccttgat tccaagaacc tgctccaacc tcaaacttca catctccaag
    20641 ttttgcaaag ccttctggag cttttgtttc ttccaacgtg tagcttccag cagcaagacc
    20701 tgaaatttgg aattgaccat cagtgttaga agtaagaact aacgcatctt cttttctctc
    20761 tacttctaca tagccaaatc tagctgctct gtaattgtca ttatatgttt tttgcgcagc
    20821 atctactaca gcttgtgctg catttttatc tgcagcattt gtataggctg ctatcgctgc
    20881 atctagcgct tgtttcgctt ctgcggcagc tttctcattt acagcttgtt gtgctgcaga
    20941 tttgagtgca agatatttcc cttgctcatt cttcacaacg aattgtgctt cttttaagcg
    21001 ttcgtctttt tggtcaacct taacgaattt tttaccatat gtttttacac gtggctcttc
    21061 aggatttatt ggttctggat tttcatcttt ccagttttta acagcaattt ttcctgtttc
    21121 agtaattgtt tggtaatctg cagaatatcc cttaattgtc cgttcaacaa atttatattc
    21181 tgtattatta tcaagacctg tagcagtaaa tgtccaatta tttgtttgat tcaatacaat
    21241 acctgtttca tgtccaggta ctttaacgac ctcacctgtc tgagcattta ccaaatcaaa
    21301 agttacttct acacctgcta taggagcatc tttagcatca gcccatgttt tagtaattgt
    21361 aagttcaccg tttttaggtt tgtttggttt tggagtgtta ccatgacctg ggttgtttcc
    21421 ataatggaat gttacatcat tcgcttctgg cacatcagca acagcaagag catttaaagt
    21481 tgcagtatac ttcaacgtaa ttgttgcttc tgcatcttta ccgttgattg cttccagacc
    21541 tttttcattt aacttcaaga taaagccatt atgacctgct tctaatgtgt aatgagaatt
    21601 atcaagcggt tgaccattat aattaacaac tacatcacca ttataatcta gaccttctgt
    21661 catttcatct gaccagaaag ctgttgcaag agttgagttt ttcggaatag ttgttgtaac
    21721 aatatacggt accttagtgc caattgctaa acctttttca cctggatcta cgaaacctgc
    21781 tgcagtatca aatgttttcg ttgctgtagg tttgtcttca gaattctttg gatagacatg
    21841 tgcattttct acaacaccat tttggtttac aagcggcaga ataattaaag caggaacagc
    21901 tttcatacct gttaaaactt taccatttgg accgacataa gtcgattctt tacgtacttc
    21961 gacaagacga tatgtccctt gtaaaacttc agtatcaaat ttaacacccg tttcagtcgt
    22021 cacaccttta ttaaccgcag tccaaaccgc atcattcgtt aaattttctt gttcttttcc
    22081 ttgaggaccc gtataactct gtaactcgaa tgcaacacca gagatttcgg ttactccttg
    22141 tggaacacct tgaagttgtt tgaactgttc aaaattttgc gaaccgtcat aacctgcagt
    22201 agtaatcgca tcagaattcc aagcgtcaag ctcttgatct gtcatcaata atttatgaac
    22261 tgtaagtgtc ttagtttttt gttccgccgc aaaaactgtt gcagctgaga acaggctact
    22321 cactgtcagt aataaggcag caagtattgt taaaaatttg ttgattgatt tcatgatttc
    22381 tccttattca tatctcttaa aatcaactgt gagcaatgta atgaattaca catgacaata
    22441 ataatccttt ttctgtgcaa gttttcgaaa acatctcccc tttcaatgat agtttactta
    22501 cgaataaaac aggtttcacc aagtaaactt aaatctcttc ttgagtgcta cttcttaaaa
    22561 gtatcgttca gaacatagat attatcattt ctacacttta cggatgtttc cgtgtgtata
    22621 atagaactcc tcccatcatc atgcaaccta tcagatagaa tggcaacatt ccgataccac
    22681 cagttcgagg atattctctc tttggaggaa taggttcatt ggtaatatag tgcttatcat
    22741 tcgtaaactc gtaacccgct ggtatatctt gtggaacgat tgaagtcaca tctctgactt
    22801 ctccatttac tatttggaag gcaacgatag gcttattttg aacgggttta taaccagctg
    22861 gttcagaatt ttcaaataat cgatatttcc catctgacag atttttaaag gtcaacttac
    22921 catcttcacc tgttctcaca ttttgataag tgccattttg atcaatagct ccataaatat
    22981 ctggataatc cggatgttgt ttttgaagac taaagaccgc atctctcagt ggttttttat
    23041 catttttatt gaccttaata aactcaatgt caccaagttt tttctctttt gaaattgtga
    23101 tttctggata cttccgcaca tcacgaatct taggaatcgg gaagtcgcgc actgtgttct
    23161 gttctacttc cttaggatgt aaggttgttc gaccattggt atcataaaat ttattgctta
    23221 caaactcatc attcaaacga acattgtagg tcaacgtaac tttttcatcc gttccaaggt
    23281 acagacctgt tacacgaatc cttttctcag tcgtatcata gagcactttt gcatttttta
    23341 acaaaccacc atcattttgt ggaccaccta cagcttgtcc attctccaag cgactaccat
    23401 cgtttgcagt taaagtgtaa tctgctggat caaatcttcc atctgtgccc aattgcaaat
    23461 caattaactc acccatcgga tctgtaatcg taccattctc aattgatttc ttttcagtta
    23521 cgatggtgtg gaaataacga ttcaactgtt ccaatatttt tgtcgtgtca gtaacattgg
    23581 tatagttttc aggtttacta gaaatacttt gcataaaact agtagccgtt gcttcatccg
    23641 taccaggatc tccgttaata ccaataccta ccgtaaagac atcataccca tcaggagcaa
    23701 tattcccgtt atagtaccat cttgtagggt caccatgatt ggtaatttta gcagtattag
    23761 aattaaacgg ataagccaga atactctctc tccaattaag ccaaatatat ccaccattaa
    23821 ttggatcgcc tataactgca taaccagccg ccttcatttc agaatatttt tcaggtttaa
    23881 ctgggaaagc tgcaggagca cctttttcat aaactgtctt atctgtaaac atctggtaac
    23941 tttgcccatc tccgcgtaca attgtatgtt ctccactagt tgcttgcgta ataaaatcac
    24001 ttaatagtat tccatcttta ttaggagatt tactaaaaaa tgcatttagt tgattttgat
    24061 atgatggagc aaacgtagca tgattaaaat taatcggata cgacatagtt gggacaccat
    24121 ccgtaatatg gaaaatgact ttttgactat tttgtctcgc ttgttgtgtc aaaatctcat
    24181 ctgccttcat caaagctttc tgagtaaaag tggcaccgaa ttggtacatc aatctatttc
    24241 catcatggtc ttctgcctcg gtaggtacct tattttttaa ttctacaatg tcattcttat
    24301 cattagtcag ctttaaataa ctataatctt tggtattggt tgtaaaactc gtctgatcat
    24361 aattccaaaa aagagaatca ttcaatcgtt ttccgttttt atctgctacc cctttttcta
    24421 ctgtaaactc ggtcccatca aagatagtgg aagcataagt cacaagcgct accctatttt
    24481 ctggatcaga tgtaatttta tcaataagag aacgtgtcgc ctcaccagct ctttccgcac
    24541 gtcgagcatt cttgtttcga atgttactca tactatttga gttatcgagc aagataacga
    24601 catccagcgg cacagactta tctttttgtt catacactgt tttcccacta accgtcaatt
    24661 cgattccata ttggttatca tccaaattat tcacttgata aattctcttt gaaagtgtac
    24721 cttctggaat cacacgttca tatggattcg gattcaacgc cttgtgctgt ccgttttttt
    24781 ccgaaccatc taccttaata atctgataag gtgtttgaac atctggataa gtccctgttt
    24841 gtggatactg gtcagataga gcctcttctc gattttctac ctgttcacct tggacagtcg
    24901 tccgaccatt cttctcaact tcaacagtcc attgtttagt agagggttta taaccaactg
    24961 gaggttgggc ttctgtcaag gtgtatgtcc caggttttat gtttgaaaat atcgcttctc
    25021 ctgtttgcgc ctctgtcctt tgcgaaacag ttgtgccatc cgtattgttt ttcaactcaa
    25081 agacggcatc tcctagaagc gctcctcctt cgcctgtctc cttaatcact acttttccta
    25141 tcgctggact ggtttcaggc gtttctgcta aagcaactat cgaagaaaaa gctgtcaact
    25201 gagatataca gcacagtcct gcaactgcct tctgaaatat ctttcttact tttttcatgt
    25261 gtgtctccct gttaagcatt attcatagaa caagttaatc cttctatgat tcataggtac
    25321 tttcaatctg tatacacgaa gaaacggatt acttatgttc tgataaaatt tatctcactg
    25381 agttaatccg ttcactaaca aacgtagcaa aagcgtataa aagtgcgtta tagctatctt
    25441 ctcaacctgg caatgacaca cttgtataca atatagta
//
LOCUS       DAVKJU010000024        24988 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVKJU010000024 DAVKJU010000000
VERSION     DAVKJU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24988
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.024"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..178)
                     /locus_tag="VW191_000971"
     CDS             complement(<1..178)
                     /locus_tag="VW191_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9623757.1"
                     /translation="MKNYGEAFRYFRKLNGYSLEYAAADFISKSQLSRFERGENEISL
                     STFFELLSNINVSIE"
     gene            complement(191..964)
                     /locus_tag="VW191_000972"
     CDS             complement(191..964)
                     /locus_tag="VW191_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU9623758.1"
                     /translation="MIDKVIEQYKSHDNLDIRVELHKKYSKNKLGFNNWIFSNYQITD
                     EVKVLELGCGTGELWKSNSDSIDKMKQLIVTDFSKDMVKSTKSVIGNRNNVNYEIMDI
                     QKISFENETFDIVIANMLLHHVNDIPKALSEVNRVLKTGGIFYCATFGENGVVNYLAS
                     LFKDEVNQDLENRTFTLQNGKRYLSRYFNSVDTLLYDDELQVTSIDDLVKYIQSFKGI
                     SEIGSLEEEIIRKRLESEFNNGMLIIPKEYGMFIARKES"
     gene            complement(1410..2165)
                     /locus_tag="VW191_000973"
                     /pseudo
     CDS             complement(1410..2165)
                     /locus_tag="VW191_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2303..3925)
                     /gene="groL"
                     /locus_tag="VW191_000974"
     CDS             complement(2303..3925)
                     /gene="groL"
                     /locus_tag="VW191_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU9623759.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVSGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(3941..4225)
                     /gene="groES"
                     /locus_tag="VW191_000975"
     CDS             complement(3941..4225)
                     /gene="groES"
                     /locus_tag="VW191_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU9623760.1"
                     /translation="MLKPLGDRVVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(4495..4890)
                     /locus_tag="VW191_000976"
     CDS             complement(4495..4890)
                     /locus_tag="VW191_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU9623761.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(4968..5729)
                     /locus_tag="VW191_000977"
     CDS             complement(4968..5729)
                     /locus_tag="VW191_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU9623762.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(5762..6388)
                     /locus_tag="VW191_000978"
     CDS             complement(5762..6388)
                     /locus_tag="VW191_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEU9623763.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(6404..6721)
                     /locus_tag="VW191_000979"
     CDS             complement(6404..6721)
                     /locus_tag="VW191_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEU9623764.1"
                     /translation="MIQPASLEELASLVEKDGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRVDRDQYIDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(6718..7017)
                     /locus_tag="VW191_000980"
     CDS             complement(6718..7017)
                     /locus_tag="VW191_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEU9623765.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLLGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7267..7455)
                     /locus_tag="VW191_000981"
     CDS             complement(7267..7455)
                     /locus_tag="VW191_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9623766.1"
                     /translation="MKASTWVYDKGEWYYVSSSGAMLANDWVKDNGKWYYLASSGKML
                     RNTYTPDGYYVGNSGAWQ"
     gene            complement(7706..8380)
                     /locus_tag="VW191_000982"
     CDS             complement(7706..8380)
                     /locus_tag="VW191_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623767.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(8386..8832)
                     /locus_tag="VW191_000983"
     CDS             complement(8386..8832)
                     /locus_tag="VW191_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623768.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(8946..9449)
                     /locus_tag="VW191_000984"
     CDS             complement(8946..9449)
                     /locus_tag="VW191_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9623769.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(9446..9808)
                     /locus_tag="VW191_000985"
     CDS             complement(9446..9808)
                     /locus_tag="VW191_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HEU9623770.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFLQAPYYVPERIAITGKICGVHIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(9874..11733)
                     /locus_tag="VW191_000986"
                     /pseudo
     CDS             complement(9874..11733)
                     /locus_tag="VW191_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(11772..12221)
                     /locus_tag="VW191_000987"
     CDS             complement(11772..12221)
                     /locus_tag="VW191_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU9623771.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIDQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            complement(12345..13061)
                     /locus_tag="VW191_000988"
     CDS             complement(12345..13061)
                     /locus_tag="VW191_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9623772.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(13942..15357)
                     /gene="ply"
                     /locus_tag="VW191_000989"
     CDS             complement(13942..15357)
                     /gene="ply"
                     /locus_tag="VW191_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEU9623773.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(15368..15700)
                     /locus_tag="VW191_000990"
     CDS             complement(15368..15700)
                     /locus_tag="VW191_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623774.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(15771..16379)
                     /locus_tag="VW191_000991"
     CDS             complement(15771..16379)
                     /locus_tag="VW191_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623775.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(16392..16850)
                     /locus_tag="VW191_000992"
     CDS             complement(16392..16850)
                     /locus_tag="VW191_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEU9623776.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            16975..17374
                     /locus_tag="VW191_000993"
                     /pseudo
     CDS             16975..17374
                     /locus_tag="VW191_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(18139..18399)
                     /locus_tag="VW191_000994"
     CDS             complement(18139..18399)
                     /locus_tag="VW191_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623777.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFFDG"
     gene            complement(18458..19009)
                     /locus_tag="VW191_000995"
     CDS             complement(18458..19009)
                     /locus_tag="VW191_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623778.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNSEFPSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHVKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(18999..19196)
                     /locus_tag="VW191_000996"
     CDS             complement(18999..19196)
                     /locus_tag="VW191_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623779.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(19200..19994)
                     /locus_tag="VW191_000997"
     CDS             complement(19200..19994)
                     /locus_tag="VW191_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623780.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(20402..20827)
                     /locus_tag="VW191_000998"
     CDS             complement(20402..20827)
                     /locus_tag="VW191_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623781.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDNNKIIGKGEAASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(20956..21339)
                     /locus_tag="VW191_000999"
     CDS             complement(20956..21339)
                     /locus_tag="VW191_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623782.1"
                     /translation="MYHLYYDEVKKSSVSLILIGEGDETERKADQLASYFLIFPSSLY
                     RMVEEIRENANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIE
                     TASRLGYDTSLYRPLSESKKEMVLG"
     gene            complement(21361..21567)
                     /locus_tag="VW191_001000"
     CDS             complement(21361..21567)
                     /locus_tag="VW191_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623783.1"
                     /translation="MDLSNKASNLRKKLGADGEAPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMSLGR"
     gene            complement(21554..21727)
                     /locus_tag="VW191_001001"
     CDS             complement(21554..21727)
                     /locus_tag="VW191_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9623784.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLYLSLNQSMIAK
                     MEKGERNITNGFK"
     gene            complement(22086..23042)
                     /gene="lytA"
                     /locus_tag="VW191_001002"
     CDS             complement(22086..23042)
                     /gene="lytA"
                     /locus_tag="VW191_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEU9623785.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADRPEFTVEPDGLITVK"
     gene            complement(23418..24788)
                     /locus_tag="VW191_001003"
     CDS             complement(23418..24788)
                     /locus_tag="VW191_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU9623786.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
BASE COUNT         7571 a         5470 c         4275 g         7672 t
ORIGIN      
        1 tctctatcga aacattgata tttgataata attcaaaaaa tgttgaaaga gaaatttcat
       61 tttctcccct ttcaaatcta gaaagttgag attttgatat aaaatcagct gcagcatact
      121 ctaaagaata tccgtttaat tttctaaaat atcgaaaagc ttctccgtaa tttttcacac
      181 tttcattccc ttaactttct tttcgagcga taaacatacc atattcttta ggaatgatca
      241 acataccatt attaaactca ctttccaatc ttttacgtat tatttcctct tctagtgaac
      301 ctatttctga aattccttta aacgattgga tgtatttaac taggtcatct atactagtta
      361 cctgtaactc atcatcatag agtaatgtat cgacagagtt aaaataccta ctcaggtacc
      421 ttttaccatt ttgtaaagta aacgttctat tttctaaatc ttgattaacc tcatctttaa
      481 ataaacttgc caaataattt acaactccat tttcaccaaa tgtagcacag taaaaaatgc
      541 ctccggtttt taagacccta ttcacctcag agagagcttt aggaatatca ttcacatgat
      601 gtagaagcat attagcaata acaatatcaa aagtttcatt ctcaaaagaa atcttttgaa
      661 tatccattat ctcataattg acattgtttc tatttccaat cactgacttc gttgatttta
      721 ccatatcttt agaaaaatct gtaacaatca actgcttcat tttatctata gaatcactat
      781 tacttttcca caattctcct gtaccacatc ccaattctag aacctttact tcatcagtga
      841 tttggtagtt agaaaatatc caattattaa atcccaattt attttttgaa tacttcttat
      901 gcaattctac tcgaatatca agattatcat gacttttata ttgttcaatt actttatcaa
      961 tcattttaaa tgacctccac tctataattt ggcattatat cacaagaata aagatagtgt
     1021 taaataagtt atataaatgc aaaaaaggaa tattccgtta tagcagagtt gtaacacatt
     1081 tctataaaga aatttattcc tacgactcag tttggcatgc ttaacttcct aactcaaaaa
     1141 caaagcatcg attaatttat ccgttcttct tttgaaacag atttgaactg ataattgctc
     1201 gttttaattt aaaaagctct tttgagcaaa acttcatttt tttaagagtc caacaatttc
     1261 tgtctatcgc ttttctacta gcaaaaaacc taccttgagg caagttttca tttatatatc
     1321 taatttcgta acagtatagt aaaatgaaat aaggacagtc aaatcgattt ctaacaatgt
     1381 tttagaagta aaggtgtact attctagttt caatctacta taaatttgat atgagaatag
     1441 tctttctcaa catccttatc caacaagcat gctgtggcgt ccttcttaac ttaaaccagg
     1501 tcaatattct gggcttgggc tgcatagacg cagccacaat aacattgacg atagatatca
     1561 tactcctcac acatctctac tgaacgtttg tagccttgat ttttcttgaa atcgttggga
     1621 agatagtggg gcgtgtaaat tttttgcaca tcgattccga tgctattgat agtttgagaa
     1681 ttcttatgag gactgatggt caaggctgaa ccaaagtagt caaagcccaa gtccatagcc
     1741 acttgcgctg ttttatccag tcggtagtca aaacaaacct tgcaacggtc gccaccttcg
     1801 ggctcctcct ctagccccct aactagtttt cggtattaat tgggttcgta gggagcttct
     1861 aggtactgaa ccgtatttcc tgtccgctca ttaaaatcac taacaaattt cttggtgaca
     1921 tagacccgct tatggtattc tgccttggga tggatattag aattggcaaa atagatggtc
     1981 acatctgcat acttggtcaa atattctagt gtataggtac tacaaggggc acagcaaaca
     2041 tgcacgagaa tggttggccg ctactcattt ttttcccatg cttgtaccat tttctgcatg
     2101 acacggtcat aattaatctt ctgattgggg gtcatcttgc acagaatttc ttctacatcg
     2161 atcatgttct tctccttttt ctagtcttat tttatcatat aagttaggag agctcaaatc
     2221 aatttagaag tgaatattat aaaaagaagg gttagtcatt ccctcctttt gtgtttttta
     2281 taagttgttt tctatagaaa gcttacatca tcccgcccat catacttgga tccattgctg
     2341 gagctggggc tactggttct ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc
     2401 tggctacaga tgctgcattt tgtagggctg aacgactcac tttaactgga tcaatgatac
     2461 cttgatcaat catgttaacc cactcgccag ttgctgcgtt gaatcctata ccaagctcag
     2521 catttttcaa acgatcgata acgatagatc cttcaaatcc tgcattgtga gcaatttgac
     2581 gaacaggttc ttccaaagca cggagaacaa tattacgtcc tgttgcttca tctcctgtca
     2641 attccaaggt agcaacagct ggaatcacat tggcaagagc tgttccacca cctgaaacaa
     2701 taccttcttc aacagctgca cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt
     2761 ctttcaactc agtttcagtt gcggctccga ccttaataac cgctacacca cctgacaatt
     2821 tggccaagcg ttcttgcaat ttttcacggt caaattcaga agttgtagtt tcgatttgag
     2881 acttgataac cgcaacacgg tgagaaatcg cttcaggatt tcctgcacct tctacaataa
     2941 ccgtgctatc tttgtccacg gtcactctcg ctgcttgacc aagagcttca attgtcgcat
     3001 ctttcaactc aagaccaagg tcttctgtga taactgttcc gcctgttaag atggcgatat
     3061 cttcaagcat ggctttgcga cggtcaccaa aaccaggtgc cttgactgct actacgttga
     3121 aggttccacg aatcttgttc aaaacaagag ttggaagagc ctcgccatcc acatcatccg
     3181 caataatcaa gagtggacga ttgctttgga gaatgctttc caaaagtggc aagatttctt
     3241 ggatattgga aattttcttg tctgtaatca aaatgtacgg attttcaagg tcagccacca
     3301 ttttttcgct atctgtcacc atgtactgtg aaaggtaacc acggtcaaac tgcattcctt
     3361 ctacgacttc aagctctgtt tccataccac gtgactcttc gatggtgatg acaccgtctt
     3421 tgccaacttt ttccattgct tcagagatgt actcaccaac tttttcagaa cgagaagata
     3481 cggctgcaac ttgagcgata gcttctttat tggcaacagg gatggcgttg tttttcaaag
     3541 cttcaactgc tgcggcaact gctgtttcaa tcccacgacg aataccgatt ggatttgcac
     3601 ctgctgtgac gtttttgatt ccttcacgga cgattgcttg ggtcaaaaca gttgcagttg
     3661 tagttccatc acctgcgata tcattggttt ttgaagctac ttctgatacc aatttggcac
     3721 ccatattttc aaaatggtct tctaattcaa tttctttggc aatagtcaca ccgtcattgg
     3781 taatcaatgg tgagccaaat gatttttcaa gaacgacatt gcgacctttt ggtcccaaag
     3841 ttactttaac agtatctgca aggatatcga caccacggac catggctgaa cgggcatctg
     3901 atgaaaattt aatttctttt gacatactta ctttctcctt ctattcttca atgattgcca
     3961 agatgttagc ttcgcctacg atgatgtact tttcatcgcc atctttgaca tcaagacctg
     4021 cgtgggcttc aactaagaca cggtctccag ttttaacact tggagcaacc aagtcaccgt
     4081 tcaaggtacg aacaccttgt ccagtagcca caacttgggc tgttttggtt ttttcttggg
     4141 ctgaacctgc gagaacaaag cctccaacag tttgttcttt ttcttctact tttaagacca
     4201 cacggtcccc taatggtttc aacatctgat ttcctccata atgagataga tattattagc
     4261 actctttata cctgagtgct aattcatagt tctattgtat cacttagtca gaaataatca
     4321 agaaaaaagt ctgactttct caagataaaa agcctgagac caactcagac tttttaatgt
     4381 ttatactcaa tgaaaatcaa agatcaaact aggaagctag ccgcaggctg ctcaaagcat
     4441 tgctttgatg ttgtggataa agctaacgtg gtttgaagag attttcgaag agtattaaaa
     4501 tggcaattct tcctcttcca agactaaatc tgccaaatct tggcctgcat tattttcacg
     4561 catggcacgt tgagcacgac tttccaagag ttggaatcct gttacaagta cttcggtcac
     4621 gtagttcatt tggccatttt tctcaaagcg acgggtacgc aattctccat caacggaaat
     4681 gagactacct ttggttgcgt agcttgccaa agtttctgct agtctgcccc ataggaccat
     4741 attgacaaaa tcagcttcac gttcaccgtt ttggtctttg taacgacggt tgacagcgat
     4801 agttgctcgc gctaccgact tgtcattgtt ggttttgtgc aattctggtg tagacgttaa
     4861 acgcccaatc atgataactt tattatacat attttcttcc tcctacttat ctattcgtag
     4921 gaaatcaaaa aaagttacag aaatttgtaa cttttcgagg aaatttctta ttttttatga
     4981 atcatgaaac ctgtcgcctg ttgattggcc ataatggtca tatctgtaat ctgaacacgg
     5041 cgaggctgac tggtcacata gactactgtg tctgcaatat cctgagcttg caaggcttct
     5101 attccttggt aaacggacgc agctcgctct ttatcaccat gaaaacgaac agttgagaaa
     5161 tctgtttcga caatcccagg ctgaatggtt gtcaccttga tatccgttgc gatggtatca
     5221 attcgcagtc catccgaaaa ggtcttaacc gcagccttgg tagctgagta aacagcggca
     5281 ccagcatagg cgtagattcc tgcggttgac cccatattga taatatgacc ttgattgact
     5341 tttatcattg ctggcaagaa acagcgagta accgccatca gacctttaac attggtatcc
     5401 aacatggtca acatatccaa ctcctcatag tcttgatagg gagccaagtc aagagccagt
     5461 ccggcgttgt tgaccaagat atcaatcctc cctatcgttt ctagaatatc agagcaaaca
     5521 gtcttcacca tgaccatatc cgtcacatct agtggaaaag tccagacggt ttgatttgga
     5581 aagcttactg caaactccga cttgaggatt tctaatctgt ctatccgtcg tcctgttaga
     5641 acgacatcct caccctgctc cagataagca cgcgcaatcg cttcaccgat tcctgaggtt
     5701 gctcctgtaa tcactacatt ttttgccatc ttatttcctt ctatcagatg ctgacaagtt
     5761 cctaggcagt ccagtgtttc gctgggtcga acggtgttcc gacaacttgg tcttctgata
     5821 attcaagcac cccacgtttt tgtggagcat ttggcagatg caattcacga ggactgcaca
     5881 tcataccaaa actcttttca ccacgaagtt cacctgggaa aatgagattc ccttttggca
     5941 tcatagctcc aggaagcgcg acaatggttt tcaaccccac acgcgcattg ggagctcctg
     6001 caacgatttg tacagtctta tcacttgcga ctgcaacttg gcagatgttg aggtggtcac
     6061 tatctggatg ggctaccatc tcaacaattt caccgacaac aaacttaggt tccttatcat
     6121 taacaatttc ttctgtaaaa ccttccgcct gcaactcttg gttcaaacga gcgacttgct
     6181 catctgtcaa aaagacttga ccgcgctctg caatttcaaa taaacttgaa acttcgaaaa
     6241 tattccaagc cactgtttcc ccattatctt tgagaaaaac acgggctacc ttgcctttgc
     6301 gctccacatc cagtttggca tctccgctat ttttcacgat gaccataagg acatcaccga
     6361 catgttcttt attatatgta aaaatcattg tttccttttt ctcctatttc agtcctgcta
     6421 aaaagtcatt gatttgttgc ttgcttttac ggtcgcgatt gacaaaacga ccgatttcct
     6481 tgtccttttc tagaacaaca aggctaggga ttccatatac atcccagagt ttggccaaat
     6541 ctatatactg gtctctatcc actcgaataa aggtaaactc tggattagtc tcctcaattt
     6601 ctggtaaggc aggatagata taacggcaat cgccacacca gtccgccaca aaaaggaaga
     6661 ccttcttacc gtctttttct actaaagatg ccaattcttc taaacttgct ggttgtatca
     6721 taagacttcc tcctcataga ctaggtcttc attttcatag acaaaagtat aatgacggcc
     6781 atcctcaaaa atgacgccac caagcaagct ctctaaactg ctttcataga cttgaacata
     6841 gagggtcgca atttccccca tgtctgaaaa atggtctcgt acaatcgctg tcaactcttc
     6901 ctgagtcttc atgagcttat ggtcatctgc aacttttttc gtagcaaggg caaggctccc
     6961 aataccaagc agagccagac ctgccatcca cattttttta gctttcatac cattcatttt
     7021 aacacaaaaa aggctttagg acaaatgagg aaacagcaaa aaagcaagta aaaagcctct
     7081 tcctttaagg aaaaggactt cttatactca atgaaaatca aagaccaaac taggaagcta
     7141 gccgcaggct gctcaaagca ctgctttgag gttgtagata gaactgacga agtcagctca
     7201 aaacactgtt ttgaggttgt ggatgaagct gacgtggttt gaagagattt tcgaagagta
     7261 ttattcttat tgccaggcgc ctgagttacc aacgtagtaa ccatcaggtg tgtaggtatt
     7321 gcgaagcatc ttacctgatg aagctagata ataccacttg ccattatctt tgacccaatc
     7381 attcgctagc atggctccag aagaacttac ataataccat tctcccttgt cataaaccca
     7441 agtgcttgct ttcatggctc ctgagcaatt aaaggttcaa aaaactgtcc aatatcattc
     7501 atttttttaa agcattttac actacatcat ccacgctgta ggacatagat acatcaaacg
     7561 taagagccaa aatatcgaaa aatatacaac ttttaaaggc aacatttcat tttttaaagt
     7621 ctaacaacca tagaactgaa atcagagcaa ggcatcaact tcctactctt agtacgtcta
     7681 ttttgatggt tgaagtgaag attttttatt taacgtagac aagctccaac ttcccaaaag
     7741 ccacatctcc acggataatc aaggttttgc tggttggggc tacaggagcg tcagccttag
     7801 ctgcaccaaa ggaggtttct actttcaaat ctacacgcca gtgttgtgga acatagacgg
     7861 ttgcattccc gaaggccact tcaatattta aagttgcaaa atcacctagc atctctgcat
     7921 tatcatagta gattttagca tccccaaaag cgacttccac ttggtcatct acgagatctt
     7981 gatcttgctt atagaaggtc ccgctaccaa aagcgacttc cttatccgtg acgactgttt
     8041 ttttcccatt gtaccaccat tttttttcat tccagaactt acttgaatgc gtcagataac
     8101 caacaccaag taccaccaag atgctagccc aaataagaga atgattggta actggtaaca
     8161 agtcataagc gtaatttgca atgatgagcg ccagtaaacc tgtaaaaact gccgaagtga
     8221 gatgacgtct aaggatggac tcaatggact tataagcaaa aaaaacaata cctagtaaag
     8281 gccatatttt accatccaaa gaaggaattc caaaattccc ttgcagcaag atccaagctg
     8341 ctaaaaccaa taaaacaata ccaaatgctt tctttttcat gttttttacc tcatgtttct
     8401 tagattttct tttaggaggg attggtaatg ccgtgagaca tgaacctcct tgtgcgtctg
     8461 ataaaaggaa atggtgcccg ttcctgaaaa ggacttgtcc actgagtaaa tctgacggat
     8521 gtttgcgatc gttgacttgg aaactcgatt aaaatagcga ggcaagatag actccaactc
     8581 atagagcttg aggcgaacct cataagcttc cttctggtta tgagcgtaga tcttgctccc
     8641 ttctgtttca aagaagagaa tttctgacag gtctagataa tattcacctg tccccttgta
     8701 aaaagtcaac ctcggagcct ttgactcttg caagagccgt tgcaagtctg caatttcatc
     8761 tgtcaaagtg ggggtcttga tgacaatttc agtctcctct aaattgccgt caatctcaat
     8821 tcgtaacttc atcacatctc ctttctgact ctactatatc aaagctaagt taggaataca
     8881 agggcaaaaa agcaagtggt caatttgaag cagtaagtgg ttgtaggact agcttaactt
     8941 acatgtcaga ataggaagaa aaggaatccc cacaccagac cacaagcata gccaaccaag
     9001 acatccttgg gataatgaac accccctaaa accctcacca accccagcaa agctgaaaag
     9061 atcaagcata ctaagccaat aggtagacta gcatgcagac aggccatgga gatgatagtt
     9121 gcagaaaaga catggcgact gggcatcgac cgtcccgaac tatccttatc aagaagggga
     9181 cagatatccc aagtttcata aggtctcggg tgattgattt tcttacgaaa cagggacaag
     9241 gccacaaaac ctgatgcagg aataagcaca taaaccccaa cttgctgacc aagtcctagt
     9301 tgcaggtaag tggtgactag caagatcaga tagaccaggg gcatggcgac tgtcatcaaa
     9361 cgattgaaac tgcgtaatag gaataaaaga attggacgac tagttagtct ggagctgata
     9421 tttcgatacc attcttgata atttttcatt tatgcttctc atacttttct agaacttgtt
     9481 gagatgtttt ccccttggca agccaatcca ctagcttgtc caaacgacgt atttcctgca
     9541 tcagaggatc atcaatttcc tcaatatgga caccgcaaat cttccctgta attgcaattc
     9601 gttcagggac ataatatggt gcttgtagaa aaaaatctcc gtaagtaaca tccctatcca
     9661 gacaagcaag aacgtcactc acttcatacc ctgtcaacca actggtcact tggtgaactg
     9721 actctgcctt tccacccttt cgctcaactt ttgcaatcaa ggctaagtag acagcagcaa
     9781 atttcatatt gtataatttc tgactcatac ttctctcttt ctatgtgtac tagtataaat
     9841 aagaaaaaga aggccgtcaa gccttttttt gatttattct tctgcttcat cttctgtaaa
     9901 ttgactattg tacaagtcag cgtagaagcc accttgcgcc atcagttcct catagttgcc
     9961 ttgctcgatg atatttccat ctttcatgac caagatcaag tctgcatttc ggatgattga
    10021 caagcggtgg gcaatgacaa aggatgtgcg tccttccatc aaacggtcca tggctttttg
    10081 gatcaattcc tctgtccgtg tgtcaacaga agaagtcgcc tcatccaaaa tcaaaagcga
    10141 tgcatcctta agaagggcac aagcaatagt caatagttgt ttttgtccta cagacaaggt
    10201 cacggtgtca tccaagatgg tatcatagcc atctggcaag gtcataataa agtggtgaat
    10261 tcccacagcc ttactagctt ccatcattcg ttcatcacta atccctattt gattatagat
    10321 gagattgtct cgaatagttc cttcaaagag ccaggtatcc tgcaagacca ttgaaaaggc
    10381 atcatgcact tctgaacgcg tcatagcctt ggtatccaca ccatcaatgc gaatacttct
    10441 cttatcaatc tcatagaatt tcatcaaaag attgacaatg gttgtcttac cagccccagt
    10501 cggcccaaca atggcaaccc tttgaccagc atgagctgtc acagagaagt catagtcttg
    10561 aacattgaca ccgtccacca gaatttctcc tgctgacacg tcgtagaaac gtggaatcag
    10621 attgaccaga gttgatttac cagaacctgt tgacccaata aaggccactg tttgaccagt
    10681 ttctgcttta aagctaacat gttcaataac tgcctccgaa tttgccgcat agcggaaggt
    10741 cacatcctta aactcgacct gacctttgaa gttttcatca gtcagctgca cttgaacagg
    10801 gttttggata gaagaatgca aatctaaaac ttgattaatc cgcttagcag agaccatagt
    10861 tcggggaaga acgatgaaga gtgctcccat gagaaggaag cccatgacaa cctacatggc
    10921 ataagacatg aaaataatca tgtcactaaa gagaggcaga cgcgctatcg gagcagcgtc
    10981 gttaatcaca taggccccaa tccagtaaat cgctacactc aaaccacttg aaatccccat
    11041 catgatagga ttcaaaatag ccataagacg gttgacaaac aaattcaaac gggtcaattc
    11101 atcatttact gctgcaaatt tttcattttg ataatcctct gcattgtagg cacgaacgac
    11161 acgaatacct gttaaactct cacgagtgat actgttcagt ttatctgtca acccctgaat
    11221 caaggactgt tttggaaagg ctagcgtcat caaaacggtc gtcatcagga cgttgacaat
    11281 cactaccaca agtacggccc agagccagta ttctgaatga cctaaaatct tcccaatagc
    11341 ccagatagcc ataattggac cacgcgttac cacttgcaag cccatagtaa tcaacatttg
    11401 aacttgagta atgtcattgg tagtacgcgt caagaggcta ggaattgaaa atttcttaat
    11461 ctctgtctgc gagtaatcca aaactcggtt aaaaatatca cttctcagcc tactagtata
    11521 agaagccgcc actcgggatg caaaaaatcc aactgcaact acggacaaga aggcaagaaa
    11581 ggacattccc atcatcatgc ttgccggctg ccacaactca tctaaattag tttcttgact
    11641 acctagcaaa tccgtaattt tcgagatata ggtcggcact tccaactcta gatagaccga
    11701 aaagcaagta aagagaatgg ctagtaaaat catcccccat tcttttctac taattctttt
    11761 ggctaatttc tttattctct cctcctattc ccttgatatt ttgcctgtag ttgaccgaga
    11821 accttctcaa aaatcagtaa ttcatcttca tcaatatctt ccatcaactg cttgtctatg
    11881 cgttcaaaaa aagccttaac ctgttgcatc tgagaacgtg ctttgtccgt cagacgaaca
    11941 aacttagccc gcttatcaac aggactcgcc tccaattcca ccaaaccatt ttgcactata
    12001 cgcttaacca gattactagc aacagacttg gtaatattga gttcctgatc gatatcttta
    12061 atcaagacca agtcttggtt tttctcgcga ttatccaaaa aatgcacaac ctgaccttgc
    12121 ggcccaccca taaattcaat gccgcaacgt ttggcttcct tttgcaccat caggtgaatt
    12181 tgatgaccaa aacgcttaaa gactaacatc ggtttaccca taatctcccc cttctaaata
    12241 aaaatagttc tctggagaat aattaaattt ctatgagaac tattttcttg attaaaaaaa
    12301 tcccaagtga ttttctcact taggatcatg ttctataggt taaattaaaa cccatctacg
    12361 ttcgtataaa tcttttggac gtcttcgtcg tcttcaagaa cgctgtaaag tttttcaaag
    12421 gtttcaaggt cttcgcctga caattccact tctgactgag gaatcatttc caattcagtc
    12481 acttggaatt cttcaatacc agactcacgg agggcaacga tagccttgtg aaggtcagtt
    12541 ggcgctgtgt aaactgtgat tgtaccttct tgtgcttcta cgtcatccac atccacatcc
    12601 gcttcgagca attgctcaaa gactgcgtcc gcatcttcac ctccaaatac aataacacct
    12661 ttgttgtcaa agaggtaaga aacagaacct gaagcgccca tgtttccgcc gtttttacca
    12721 aaggctgcac ggacattggc tgctgtacgg ttaacgttag aagtcaaagt atccacaatt
    12781 agcatagagc catttggccc aaaaccttcg taacgtcctt ctgtaaaggt ttcgtctgtg
    12841 tttcctttgg ctttatcaat cgctttatcg ataatgtgtt ttggcacttg ggcttgttta
    12901 gcacggtcga taacgaattt caaagctgag tttgattctg gatctggatc acctttttta
    12961 gctgctacat agatttctac accaaatttt gcatatactt tagagttggc tccatcttta
    13021 gccgttttct tggctacgat attggcccat ttacgtccca ttaggaatct ccttttttca
    13081 cattttaatc tttcttatta taacacaagt ttttttgatt ttcactagag gaaatggatt
    13141 ttattagcaa atcaagctag gatagcactt tacctgctaa gatggtcttg cctttctatc
    13201 tttatcaaca gacactcatc cacattcaaa aaacaaacta gaccattatc tgcaaataga
    13261 aagtttcagc caagtttgac aaagtcagct caaattactg tttgaagttt gtagatataa
    13321 gcgacaaaaa caatcatact gcaccttttg ttgacagtct actccagaca tatcatagtt
    13381 caagtaaata ctttgaaatt caacagttct tataggcgct attgtattct aagaaatcaa
    13441 tagaagagtt tctaagcaaa cctctaatac tcaataaaaa tcaaagagca aactagaaag
    13501 ctagccgcag gttgctcaaa acactgtttt gaggttgcgg atagggctga catggtttga
    13561 agagattttc taagagtata atttacgtgt tcctaagatg gagaagttag actagtacac
    13621 tggcacttct aaaccattgc tagcaattga tttgttcata tttaatttca ttttttccat
    13681 aaatgggtat tagatataaa cagcaaaata tttccgatac gtgtcattct tgaatttcca
    13741 atcatctaaa acaagtaaag gataatcaat cccctgtata tcaaggaatt ggctaccctt
    13801 tttacttttt tacacatcct gtttgataga ctcattttaa catcacgagc atactccaat
    13861 ggaaatcgct aggcaagaga taaactttca gatatccgca gagagatcat cgcctctttt
    13921 tgtcgcaagc attctcctct cctagtcatt ttctacctta tcctctacct gaggatagag
    13981 agttgttccc caaatagaaa tcgtccgctt acgcactagt ggcaaatcgg ttttttcata
    14041 aaccgtacgc caccattccc aggcaagccc ggtacactct ctaattttga cagagagatt
    14101 acgaacattc ccttttaaag gaatactagt ggtaaagtga gccgtcaaat cctgcccatt
    14161 tctgtcccaa gccttaggag tcaagacttc cttaccttga tgatcatagg ataattcatc
    14221 ccaagtaata taatattggg caacataggc accactatga tccagcagta aatctccgtt
    14281 tctgtaagct gtaaccttag tctcaacata gtctgtactg ttttgaaagg tcgcaactac
    14341 attgtcacgt aaaaaagaag ttgtatagga aatcggcaag cctggatgat ctgctgtaaa
    14401 gcgactgcct tcttgaatca agtcctctac catatccacc ttgcctgtta caactcgggc
    14461 acccgaactt gggtcgcccc ctaaaataac cgccttcact tctgtattgt ccaaaatctg
    14521 cttccactct gtctgaggag ctaccttgac tccttttatc aaagcttcaa aagcagcctc
    14581 tacttcatca ctcttactcg tggtttccaa cttgagatag acttggcgcc cataagcaac
    14641 actcgaaata tagaccaaag gacgctctgc agaaattcct ctctgtttta aatcctctac
    14701 cgttacagta tcttgaaaca catctcctgg atttttaaca gcgtctacgc tgactgtata
    14761 ataaatctgc ttaaaattaa caatctgaat ctgcttttca cctgaatgga cagagttaaa
    14821 atcaatatca agagaattcc ctgtcttttc aaagtcagaa ccaaacttga ccttgagttg
    14881 ttccatgctg tgagccgtta ttttttcata ctgcattcta gctgggacat tattgacctg
    14941 accataatct tgatgccact tagccaacaa atcgtttacc gctccgcgaa cacttgaatt
    15001 gctggggtct tccacttgga gaaagctatc gctacttgcc aaaccaggca aatcaatact
    15061 ataagtcatc ggagcacgat caaccgcaag aagagtggga ttattctcta acaaggtctc
    15121 atccactacg agaagtgctc caggatagag gcgactgtcg ttggtagctg ttacagaaat
    15181 atcacttgta tttgtcgaca agctccgctt ctttctttcg ataacaacaa actcatcggg
    15241 tagctgatta ccctctttga tgaaacgatt ttcaatactt tctccctgat gggtcaagag
    15301 tttcttttta tcgtaattca tagctagtat aaagtcattt actgctttat ttgccatctt
    15361 ctacctccta ataagttcct ggattgagtt gcataaactc agacttgttc agcgaaatca
    15421 gccgtggttg gactaagtga tccaaaattt cctcgtacaa ttcttctgag acattgcgtc
    15481 gccgtctggc tagataagaa gtcggaataa ccgtattatc caacataaat accttatcta
    15541 agtcaatcaa ggttggtctt gtaaaaggat tacgagctag atccggctct tctatcataa
    15601 agttcttgac caaacgtctg gtcaagagag ctggtttgaa ggtctgattt ttaaccaact
    15661 ctttgttttt agtcatgctg ttgtcaatac agatatacat atgattcttc acagccaaat
    15721 cgctactaat agtcggaaaa ggcaaataaa gagctacaac atctcctctc ttaatcaagc
    15781 aagagcaccc ccttttctcc taatgtaaca tagacaggat tgaccaagtc ttctgattga
    15841 ctcagaattt ccaaagtttg agtttggcgc gctgtcaatt tagtagcatc ttgtctcttc
    15901 aatacaaaat gcttgtcgcc aataaccttg acaatataat ccttctccaa agctgactgg
    15961 taaatccaca tcagatgttg tctgtcctga gaactcaaga gagaaggatt ttcaagcctc
    16021 ccgatagtct gataaaaatc aaaaacagga gctaactcct gccaatctga ttggctagtt
    16081 gtcaaggcta gaaaaagggc tttgcgagct gatacttctt ggttagcctt gagagttact
    16141 ttcccctcca agttttttag aaatcgggaa actccagaaa gcaaattttt ctctaactgc
    16201 gagaaataaa aacctttcgt tcccagacat aagtctttca tgtcgctttc tctagcaaat
    16261 aagagctcaa acatttgata gtaaaagaaa aatatctggc actgggtcgc gctcatcttt
    16321 tccttatcgg cttctttttt taaccagagc aagggcgaca ggtaactgga ttgagacatt
    16381 tcctctacct cctactcttt tttaactgga gcatctgcac tagctgccac ttcttttgac
    16441 tggatacttt cccactggtt aatctcctct gagataagac cttcgcatgt cttgacaaat
    16501 agggcaaaag ccttggtctt tcctgcatat ttctccgttt ggcattgata gaggaatttt
    16561 tctttctcca ggagttgcgc agttttttgg taagaaatcc aattttcctt tgcattatac
    16621 aaattgataa tcccctcaca cagcaagccg agactggata aggcaaccga aatcaaacgg
    16681 tagcgatcac ctggcatagg aatagcacaa aagacagcta tgaggaaacc tgccacgatt
    16741 tctgttattt ttaatacctt atagcgccta cgatgttgaa cgcttttctt taaaaaatga
    16801 gctatctgta cgtctaatcg ctctgtcagg tacatttctt ctggcgtcat attcgtaact
    16861 cctttcattt actttgataa ccatagtata acatatttga atatcaaagt atagtaaaaa
    16921 ctggactata acttattaga ctccctgcga aacaaaatat ggtatagtag ttctatgaat
    16981 gatgaagcaa gtaaacaatt aactgataca cgatttaagc gtcttgttgg tgttcagcgt
    17041 accacttttg aagagatgtt agctgtatta aaaacagctt atcaaaaaag tcggacaagc
    17101 tggtaaaatc ttggctgaca gtaattatca agggctcatg aagatatatc ctcaagcaca
    17161 aactccacgt aaatccagca aactcaagcc actaacagtt gaagataaag tctataacca
    17221 tgcgctatct aaggagagaa gcaaggttga gaacatcttt gccaaagtaa aaacgtttaa
    17281 aatgttttca acaacctgtc gaaatcatcg taaacgcttc ggattacgaa tgaatttgag
    17341 tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta ttatatatat
    17401 gcacagacat atgaaatctt tttgatacag ccttcttttt acatagagag acagcagtta
    17461 tgatatattt atccattcac aacatcttct gtaccaatac tacaataatt ggtacatttt
    17521 gattacacaa tcgtttcaaa aaatatgatg gaagactgag tattttatag taattccctt
    17581 cctatcaact agatcaacca gttgagtgtt gaatgttgaa acaccctagc ctcagcaaag
    17641 gactatgatc agcatttcct agccaatagg acgctccaaa tcacctattc taagtccatc
    17701 actagcttgg tcagtaagat acagactgca aggcaagact gacgtggttg atgagatttt
    17761 ggaagagtat tacaatcata gattcaccag tagacttgac cgcagattta actatagtag
    17821 attgaaacta gaatagtaca cttctgcttc taaaacattg ttagaaatcg atttgactgt
    17881 cctgatcaat ttgtcctgtt ctcgtttcat tttactatac taaaaaacca cctgaaacaa
    17941 atcaagtgac tagaggataa aaacaaagac tgggcaaaaa gttttgaaaa gcaaaaaacg
    18001 cataatatca ggtcttgaaa aaccttgata ctatacgttt gattgtggga agattgactt
    18061 cattttctcc tgaaattgaa gttttttcta gtttccaggt atagacgaat tcaatatagc
    18121 catctcgcta caattatttt aaccatcgaa aaaatttttc agaatttcaa aaatttcatt
    18181 ttgatccaat gaaatgtcat tattttgaag aatagttgac ttcacaggct ctaattctat
    18241 cacaaagcgg tgcatgtctg cttttaccgc actacttgat gttatagccg ataacttttc
    18301 atctcctagc aaggaagctg tcaaacgggt aaggtcattc aaatgctttt taatggactt
    18361 acttaaacct tgagcacctg tggcagagcg ctccctcata tccagccaag ctttagcctt
    18421 aaaaataatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagt tgcaggctgc
    18481 tcaaaacact gttttgaggt tgtagatgga agctgacgtg gtttgaagag attttcgaag
    18541 agtatagacc acaattactc aataccgaat acccctgaat ggtttctttt acatgcacca
    18601 atatattata ataatcttca tccaataata aggctgataa actagcatct tggtcaaaat
    18661 ataagggaat ttctcgaccg tccttcttta atggatattc tggtaagata ctaaatagtt
    18721 caatcataga aggaaactca gaattagttg ttgtaaatcg aaaaagctgc gctttctcat
    18781 ctttctgact tccttgatac tctcccaatt ctaaaaaatg attcaaggta gtataaaatt
    18841 ccttattttt tacttcatca atgatgacca tatcataatc ttttgttgtg cgacttttaa
    18901 atccttgcga atccaatacg atagaggtag cagttccccc aatcagaaca taatagttct
    18961 gaaaatccgc aaacatttct tgaaaaataa ctttcgtatt agctggcatc atcttctccc
    19021 agatactgta atatcatatt ttctagtgct tcactctctt cctctatacg tgggtcatca
    19081 tcatctttta aggtcaaata aagagaaatc ggatctacaa attgtttatc atgattattt
    19141 ttaaaatcat tccaaaactc agatacaaaa ggacgatatt tccatatctc tagcatcttt
    19201 cattttaaaa catgctgaga aagtggcaag gataactgat tgaattttct ctgccatatg
    19261 acatagctaa tattttcatc cgtttcagct aaaaaagttg aatgcgacaa agcataagca
    19321 ccaccatata gaaggttaga aacagatttt atctgcttta tatcgccatc tggcaataaa
    19381 atccgttttt tgatgggatt aaataaacat gacacggatt ttaagaataa ttctttcttt
    19441 gacaccgtat atgtgtaaag cttattttgc ttgtttaacc aatataaagc tttaaaagtc
    19501 ctcaaacacc tataaattgt tgagtttgga agtccagtga cttgtgaaag caaatcaaca
    19561 tctactactt tttgaccttt tgtcaataaa aaggcaatcc acgttaattg ttcgctaggt
    19621 gttaattcct tagggacttc agtatcattc gcattgagta ctagtcccaa tggagggaag
    19681 aagaggtttc ccttaaagtc tacaaacgga actctagctt gaagtaattg ctttttttca
    19741 ctgtctgata acttcgaaaa caccaaaaca acatccatat tggctttttc acccatagtg
    19801 cgagcctgag taacaaatga actcaaactc ccccttctct tttctttaat caataaaaat
    19861 gattccttat gaaaagtcat ttgagtatac tcaaaccttt ttacaaaact gattggtaaa
    19921 gtcaaagtca gttcctcaat actgacatca acataaaagg attgaaaaat tgacattatc
    19981 tttttgacat tcatgttttt tccttattct atcattttta ttatatttta tcattcggta
    20041 taataaaata taagtttttt gataaaataa ccaaaaacta ctcgctatcc cctctcacat
    20101 taccctacat atcgtttgac atgcgactga tagttcagga aaacttcaag gagaactttt
    20161 ctctcatcca ctatgcagga cttactattt cacttctact cctataggct caatttgaca
    20221 cttttctttt tcgaattgct catatgcctc ctgaatagtg agttcgattt atccaaattt
    20281 atttttgtct attttgatga atacttccac gaagatagtc tgaaaatgaa tttgcaccaa
    20341 ttttccttct ctttttaagc atattattcc agatatgatt gccctcttgt tcagtaataa
    20401 attacgcttt aaacgcttca atcagtatat ctcctgttgt catatgttct aaagaaaatt
    20461 cttctacata tgatttaaca tctcttaggt tattacttcc taatatccca ttatgctttt
    20521 tcgctaagga aatagaagcc gcttctccct taccaataat cttgttatta tcatgatttc
    20581 ttgttaaatc tctatataat gcgtattctt cagttccaat gtctatgctc acaatctcag
    20641 ctgaaccctt agctaccaac tgatctatcc tagattttaa atggggaatt gtaggtatat
    20701 tgatttcatc atacacctct tgtggaataa caattttacc cgaatagagc ttttctaaaa
    20761 gatgttcagt gccaacccat aaaaatactg aaatacaatc tgcatcaata aatacacgac
    20821 tagtcaaacg acaactcccc cctcttcatc tagcccatat acaatatcat atctgaaagc
    20881 atctagtaac agttcctcat acttcccttg cgaaattctg ttattttcta aaagttgttc
    20941 agttgaatta atatattatc ccaataccat ttccttttta ctttctgaca aaggacgata
    21001 taaacttgta tcatagccta atcttgaagc tgtctctata acactaatat ccatattttt
    21061 aatttcttct gcatcaaggt atccatcatt cctcaatcta tataacatag ctttatgact
    21121 gataccataa aactgaccca attttataat atcttctact tcaagatgag ttctattggc
    21181 attttctctg atttcctcaa ccatcctata cagtgaagat gggaaaatta aaaaataaga
    21241 agcaagctga tccgcttttc tttcagtttc atctccttca ccaatcaaga taagactgac
    21301 tgaactcttc ttcacctcat cataataaag atgatacagt tcatgtgcta aagaaaatct
    21361 ttaccttcct aatgacatgt ctgaattgac tgcaatgaga ctgaactgag ttcctttata
    21421 acagactccg ctaatattct ttccgagtcc ataaaatacc agcgtcaaat tttctatctt
    21481 ttgtaccaat ttaaaaatat ctatcggcgc ttcaccatca gctcccaact tttttctaag
    21541 atttgaagct ttattactta aatccattcg tgatattcct ttcacctttt tccattttgg
    21601 caatcatgct ttgattcaaa gacaaatata aagacaaata ttcagcaatc ttatcttgag
    21661 taagatttga agatattcta agttctttta ttctttttcc aacatcacat acatttatca
    21721 catacattta tcatattcat acacctctca taaaaagaat agcacacttt tgtcataatt
    21781 ttttaaataa aaaaattatg acaaaacaag gaagcaattt attgatgctg cttaaaaatc
    21841 taaaattgat gagttcctat caaaaatcgt atcttcaacc tcaaaacagt acttaaagct
    21901 atccgactcg gtttacattg tcaaatttag attttatttg agcataactt tctagtttgc
    21961 tttctgattt ttgtttaata tagtagcaaa cattgaagga aatatctcca caagaaaacg
    22021 catactatta agctttttca agacctaata atatgcgctg ttctgatttg aaagacattc
    22081 cattattatt ttactgtaat caagccatct ggctctactg tgaattctgg cctgtctgcc
    22141 agtgttccgt ctggtttgag gtagtaccag cctgttccgt ccgctgactg gataaaggca
    22201 tttgatacca tggcgccttc tttagcgtct aagtagtacc aagtgtcctt gtacttgacc
    22261 cagcctgtct tcatggcacc ttcttcgttg aaatagtacc acttatcagc gattttcttc
    22321 cagcctgtag ccatttcgcc tgagttgtcg aaccagtacc agttgccgtc tgtgtgcttc
    22381 ctccagcggt ctgcaagcat atagcctgaa ctgtcaaagt agtaccaagt gccattgatt
    22441 ttctcaaact tgtcttttgg ataagagccg tctgaatgta cgtaccagta gccagtgtca
    22501 ttcttctgcc agcctgtttc aatcgtcaag ccgttctcaa tatcatgctt aaactgctca
    22561 cggctaatgc cccatttagc aagatatgga taagggtcaa cgtggtctga gtggttgttt
    22621 ggttggttat tcgtgcaata ctcgtgcgtt ttaattccag ctaaactccc tgtatcaagc
    22681 gttttcggca aacctgcttc atctgctaga ttgcgtaaga gttcgatata aaggcggtag
    22741 tccgtcatga actcttcttt ggttgaatgg ctttcaatca gttcaaccgc tgcataggtc
    22801 tcagcattcc aaccgccccc aacgtcccag gcaccattat caacaggtcc tacctgcatg
    22861 atgcaaccgt tcccaacaat gtgcgagaaa aaacctaatt ctgggtcttt ccgccagtga
    22921 taatccgctt cattctgtac ggttgaatgc ggattcccag ttgagtgtgc gtgtacttgc
    22981 ctatatggtt gcacgccgac ttgaggcaaa tctgttctta atttactcac attaatttcc
    23041 atattctact ccttatcaat taaaacaact cattttttac aatccagaac cagaaactcc
    23101 tttatttcta ccttacaaag aagacaatct tagtcacgat taggcttgta gatagaactt
    23161 caaaacgcac tattttgaca ctgtaaatag gactgacaag gtctgcattc tatctacaat
    23221 aacaccccag actaaaaagc ttttcaaaag tatattttta cagtctctat atgtcctttt
    23281 cataaatact atactttatt atatcatata aaagaagtca aaagtctgtt aaactatttt
    23341 caacaccaaa ctaaagacga gaacacaagt tttttcgatt ttcactagag gaaatggatt
    23401 ttattcagta aatccaacta ggattgcact ttggttgcca agattgcctt gccttctttt
    23461 atcaagggat gacggaataa tgagaagtac agttgaactg tcatggcaac ccagataaga
    23521 ggttcacaaa ggataacacc cttatatcct gcccaaggaa taatcaaaac cacaaaaacg
    23581 atttttccga ttagttcaat aaagctagaa actagaggaa ggatcttttg ccccaagccc
    23641 tgcaagcaat tgcgataaat caacaagagg ctcaaaatgg gataaaaggt tgaactgatt
    23701 tgcagataga gacttccatt ttctatcaag taaccatctg tcgaactagc caagaaggaa
    23761 accaaagctg gactggcaaa aaagaggaaa atacaaacaa aaactgccca ggatatactt
    23821 aaacgactgc cgattcgaag accttgaaca atacggtcag gtcgcttagc tcctagattc
    23881 tgagaagcaa aggtcgtcat tgatgcagaa atagcggtca taggaagaag ggcaaaggtc
    23941 ataatgcgtc gagctgccgt ctgggcacta ataatcactg caccaaatgt attaacagaa
    24001 aactgtaaaa tcacactgcc gatagataca attgaactca tcaagcccat agccaaacct
    24061 tgctccaaga gatccgcgta caagcttttg tcccatttga aatgtttaaa ctgtggcaag
    24121 agttctggca cacttttacg aatataataa aagcagagaa ccgctgataa accttgcgaa
    24181 ataatggtag caagtcctgc ggattgaact cccagatgca attgcgtaat aaaatagaga
    24241 tccagaacca cattaaccaa ggcagagaaa atcagaaatc ccagggctgc tagactgtca
    24301 ccaatagacc gcaacaagcc tgcaaaaaga ttataagcaa agctgacacc tacacaggtc
    24361 acaatcatag aaatatattg ataagattga ggaagaattt ctgcaggagt atctaagtat
    24421 tgcaagagag gatacaagcc aagaaagccc agcaacataa ctagaatgct caaaagagca
    24481 cctaaaatcc aggtggctgc tactgcttcc ttgattttag tgaaattccg agccccataa
    24541 taacgagcaa tgacaatccc catgccattg ccaacaccaa gtgtaaagcc tacaatcagg
    24601 tcaaaaatcg ctgtcgtcgc tcctactgca gccaaggatt cttgaccaag aaatcgtcca
    24661 acaatcaaga catcagcagt gttatagagc tgttgaaaaa tatttgatag caagattgga
    24721 aaggtgaagc ttaagagcga gggaagaatc ggaccatgta tcaggtccac tgttcgtttt
    24781 ttattcataa agctattata tcagctttcc agaggagcga caagaaacag caaacttata
    24841 tatagcggaa tgaaataagg acagtcaaat cgatttttaa caatgtttta gaagtagatg
    24901 tgtactattc tagtttcgat ctattatagt tggttgagtt tggaatagta cgctgtagct
    24961 gctaaaacat ttctagaaat taatttga
//
LOCUS       DAVKJU010000025        24950 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVKJU010000025 DAVKJU010000000
VERSION     DAVKJU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24950
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.025"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..748)
                     /locus_tag="VW191_001004"
     CDS             complement(<1..748)
                     /locus_tag="VW191_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623787.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKE"
     gene            complement(905..1150)
                     /locus_tag="VW191_001005"
     CDS             complement(905..1150)
                     /locus_tag="VW191_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623788.1"
                     /translation="MDIYGRKTIAMKHFFAGIGEIHFLLFLLYLSMSASPIIGLVIGY
                     TKINLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            complement(1630..1842)
                     /locus_tag="VW191_001006"
     CDS             complement(1630..1842)
                     /locus_tag="VW191_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623789.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            complement(1850..2221)
                     /locus_tag="VW191_001007"
     CDS             complement(1850..2221)
                     /locus_tag="VW191_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HEU9623790.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            complement(2199..2537)
                     /locus_tag="VW191_001008"
     CDS             complement(2199..2537)
                     /locus_tag="VW191_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9623791.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            3003..3866
                     /locus_tag="VW191_001009"
     CDS             3003..3866
                     /locus_tag="VW191_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9623792.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLVQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(3918..4505)
                     /locus_tag="VW191_001010"
     CDS             complement(3918..4505)
                     /locus_tag="VW191_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU9623793.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            4747..5418
                     /gene="sdaAB"
                     /locus_tag="VW191_001011"
     CDS             4747..5418
                     /gene="sdaAB"
                     /locus_tag="VW191_001011"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEU9623794.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            5427..6299
                     /gene="sdaAA"
                     /locus_tag="VW191_001012"
     CDS             5427..6299
                     /gene="sdaAA"
                     /locus_tag="VW191_001012"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEU9623795.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            6321..6953
                     /locus_tag="VW191_001013"
     CDS             6321..6953
                     /locus_tag="VW191_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU9623796.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAVQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKDTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAITWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            7003..7170
                     /locus_tag="VW191_001014"
     CDS             7003..7170
                     /locus_tag="VW191_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623797.1"
                     /translation="MKIKEQTSKLAAGCSKHCFKVVDRTDEVSSKHCFEVVDRTDEVS
                     NHIRQGDVDVI"
     gene            complement(7256..9106)
                     /locus_tag="VW191_001015"
     CDS             complement(7256..9106)
                     /locus_tag="VW191_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEU9623798.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(9148..10305)
                     /locus_tag="VW191_001016"
     CDS             complement(9148..10305)
                     /locus_tag="VW191_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9623799.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINKNML"
     gene            10363..11529
                     /locus_tag="VW191_001017"
     CDS             10363..11529
                     /locus_tag="VW191_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEU9623800.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            11668..11994
                     /locus_tag="VW191_001018"
     CDS             11668..11994
                     /locus_tag="VW191_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU9623801.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            11981..12574
                     /locus_tag="VW191_001019"
     CDS             11981..12574
                     /locus_tag="VW191_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEU9623802.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            12567..13478
                     /locus_tag="VW191_001020"
     CDS             12567..13478
                     /locus_tag="VW191_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEU9623803.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            13540..14604
                     /locus_tag="VW191_001021"
     CDS             13540..14604
                     /locus_tag="VW191_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623804.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(14676..15536)
                     /locus_tag="VW191_001022"
     CDS             complement(14676..15536)
                     /locus_tag="VW191_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEU9623805.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKLEDYESIARLDYAKFLEVLPLSFYHQLDANQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(15811..16797)
                     /locus_tag="VW191_001023"
     CDS             complement(15811..16797)
                     /locus_tag="VW191_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEU9623806.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(17069..18544)
                     /locus_tag="VW191_001024"
     CDS             complement(17069..18544)
                     /locus_tag="VW191_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEU9623807.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(18648..19571)
                     /locus_tag="VW191_001025"
     CDS             complement(18648..19571)
                     /locus_tag="VW191_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9623808.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(19585..20514)
                     /locus_tag="VW191_001026"
     CDS             complement(19585..20514)
                     /locus_tag="VW191_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9623809.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(20833..21054)
                     /locus_tag="VW191_001027"
     CDS             complement(20833..21054)
                     /locus_tag="VW191_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623810.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(21008..21268)
                     /locus_tag="VW191_001028"
     CDS             complement(21008..21268)
                     /locus_tag="VW191_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623811.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            complement(21664..22275)
                     /gene="rpsD"
                     /locus_tag="VW191_001029"
     CDS             complement(21664..22275)
                     /gene="rpsD"
                     /locus_tag="VW191_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU9623812.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(22470..23522)
                     /locus_tag="VW191_001030"
     CDS             complement(22470..23522)
                     /locus_tag="VW191_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEU9623813.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(23519..24217)
                     /locus_tag="VW191_001031"
     CDS             complement(23519..24217)
                     /locus_tag="VW191_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623814.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(24384..>24950)
                     /locus_tag="VW191_001032"
     CDS             complement(24384..>24950)
                     /locus_tag="VW191_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9623815.1"
                     /translation="EAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRA
                     NGTQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVT
                     PMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQT
                     SMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT         7535 a         5319 c         4498 g         7598 t
ORIGIN      
        1 tttctttata aagcaaaggt aaaactttat aaaaaatatc taagtcaacc tcactctgat
       61 tctgaagcaa gtaggagtca agcaaatttt caaaaatagc taacaatatg attggtgtaa
      121 agtttttgta ttcacatgtc aagtattctg agtatcgtgc agatgatatt tcttttatat
      181 aactttttga aagcaagatt ttttcaaatt catcatctat tattaatctt ctatttccaa
      241 tccaagcggt attaccaccc aaataagatt gtgcaaatag caatatgtaa gaaaatatga
      301 acgtaaatac tgaaagaggt agaattctat gcgtaaaaaa atagacgata atggtagcca
      361 ggagtaaaga tatctctcgt gacaatcgaa ttcttttcag catctttttt aacagtccgt
      421 aaggttgtcc ttggcaatat tcttgaacta aattaatcgg aatgacatct ctcaccattt
      481 ctggaaagta gagtaagttt ataggttgaa atctccagct cccatcataa gtaaatggat
      541 atagaaccaa atacttaaaa gatttttttc ttataaactt aattatcaca atatgaatga
      601 ctgttccaat gaagtatatt aaatataaaa gtaaaaccgc catcaatata cttacaaaaa
      661 tccccttgga atcaatcaat ggaaaataaa atattgcaac tagaagcgaa taaagtcccg
      721 ctaataaata tttctgatca tctttcattc aaaacaccat attatttact aagaatacat
      781 caatacaata gagcaactct gtaagtcaat atttgataaa gcaaagcttt aaccccttcc
      841 ccagacgtta gcttttcttc aacccactac agttgacaag gagcccaaat tattgataaa
      901 aactttagtc atcatcgtct ctataaaaat attttctata cactcttgat aataccaaaa
      961 ttaatgccaa tatcatacca agagctataa taatagaatt tattagattt attttagtat
     1021 atccaatcac tagtccaata attggactag cagacatgct tagatataat aaaaataaca
     1081 aaaaatgaat ttcaccaatt cctgcaaaaa aatgtttcat agctatagtt ttacgcccat
     1141 aaatatccat caaatttacc gtctacgata tctaaaccat atgcaatggc tcctcctaat
     1201 gagaagactt cagcaacagt agccgcaata ttaagagcat tagtaatagt tgccatacta
     1261 actccaccaa tatatcttgt tatagcactt agttacctag ataagatatc ctttagcttg
     1321 cttccaaaat ttaagtctac acctataata tgagttcata ttagtactgc taaatcctat
     1381 ctatagtaat gtcaaacacc taaataaagt catctctcca attcattcaa gaaatgaaga
     1441 aaggcaaaaa atctttaaaa taagaaaagt gcatattatc agatgttcaa aaatcttgat
     1501 aatatgcgtt atatagatag aaagatttac ttaatctcat tttcaaattg agttttttcc
     1561 cagcctcgtt tttaatttat atcttcaata gaatctaatg accgagctac tgtattacac
     1621 agattaatat tatttagtct gtaatttttt taaaaaactt aatagtaaat ttgctccact
     1681 gattgctatc aatatttcaa ttggtaaaaa atagtaattc caaataaaat gagaaatcag
     1741 caatgataga aaaataacaa tatcaatttt cctgttgttg acatactctg taattttatg
     1801 atacaggaat gcaacaataa ctgaaattaa aacaaccatc ataatactat tatcctattc
     1861 tcaattgatt atcacgtcgc ccatcccagc catcaataat attggtaaaa atccaatcag
     1921 ctccagcacc aattccagca gatgcaatac ttctaataac agtatttgca aaagcatttc
     1981 caatatgaat tcctaatact cctaaacctt taacaatccc agctaccagt gcggagcgta
     2041 atccataaga tgcaagaact agttgaaggc ctccacctcc tgctgctgct aatgctgcat
     2101 atgctgcacc agtaatggct cctgtaattg cccacctggg tatatatata gcccccccat
     2161 caaggtacat catctcttct tcatcaatca caacataatt atttggtaat acgagttcca
     2221 tttattttct cctttatact atgtaatacc acaaaatgat tttgacctaa atcattctta
     2281 acttgaagaa tcgcaggaaa tgttaatttt ggagtcaaat cttccagtgc tactcgaaca
     2341 gcttttactg taaaattcaa cttattcaga cctgaaacaa taccactgac agtcgttcca
     2401 tacatgtctg taccaataat ttccctaatc ttcatgattg atagttcctt tttgtaagat
     2461 aaaagaactg ttgaaacaac tgccgcagca caatctgact ggtcatgttg catcgtacaa
     2521 ggatattttt ttaacataga aatactcaat tctttagaat taatctaact tttctttgat
     2581 gtcacccaaa cctcaatcac tgcccagtag ttcttgaatt agtttatttt caaccgtatc
     2641 attctttgtt cttgatatct ttccaagcat tgtagtacag cagatttttg cccaaggact
     2701 gagcctcctt taacaactca agctccctta cattatatcg gccagcccca tggaaagatt
     2761 atagccagac taatcaatga tataatacat actagaatac aaaatatttt tttattcata
     2821 ctttttctcc tatctttatt ttctacctac atagtacctc ttattaagct catttcaaaa
     2881 ttacgttaac ttttttcaca aatttatttc gactttcttt tttacttttt acattgctat
     2941 gatacaatat ttttaacaaa attttattgt ttgttaactt ttttcacgaa aaggagaaaa
     3001 tcatgcgtta tgatttcgga aaggtctata aagaaatacg tgagtcaaag gggctgaccc
     3061 aagaagaggt ctgtgggggt gttctctcaa gaaccagctt atcaaaaatc gaaagtggca
     3121 agacaacccc aaaatatgaa aatatggaat ttcttctccg tcaaatcaat atgagttttg
     3181 aagagtttga atatatctgt caactttatc aaccaagtca acgcacagag attatgcaaa
     3241 cctatctcaa tatgcgttcc attatcggga ccagcgatct tgtcaattta tttcaaaaat
     3301 gccaagacta cctcaagacc caccacgatc tccctataga agaaatcaga gatatgcttg
     3361 aagttgtcat ttatatccgt caacacgggg caggggaact atcagatcac gcagaacagg
     3421 ttgtcaaaaa actttggaga aaaattgaaa aacaagatac atggtatgag agtgatctaa
     3481 aaatcctcaa taccattctt ttcagttttc ccattgaata cctccatctc atcactggaa
     3541 aaatcttgca acgcttagaa gtctataaaa actatcaaca tttatatgac ttgcgaatag
     3601 caatcctgct caacctttcc acactctatc tatacaatca agataaaaac atgtgtaagc
     3661 aaatctgcta cactttacta gaggacgcca agaataagaa aagctacgat aggctggcta
     3721 tctgctatgt ccgtattggg atttgtacag acgattctaa acttgtccaa aaagggttct
     3781 cccttctgga gctaaccgag gaaacttcta tgctgtctca tctcaaaaaa gaagtagaga
     3841 tctattacca agcgaaggaa agataaaaaa gccgagggat ttcctcgact ctttacatat
     3901 tatttgttcg ttagttctta ataccagcca ttgttaagcc agaagttttt agcagcagtc
     3961 catgaaccgt aacgtcctgc aacgtaggca tctgctacac gttcttggtt ttcagctgag
     4021 tagtcaccgt tcaggtatga atctgttaat tggtaacgtc cgatataacg tccatttgta
     4081 gctgtatagc taccacctga ttctttttga gcgatccatt ctttggcttc tgcttcagat
     4141 ccgcttacag ttgaaacaac tgtttcactt actactggag tttctgctac tggagctgaa
     4201 acagtttcat cttgagcggc tggtgccgca taagtagctg gcgctggtgt tgcaacaggc
     4261 gctacagggc catcgataac caactcttga tcaacataaa tcaaatgaat gttatcaatg
     4321 tggttgtttt ctgccaattt ttcaactgtt gtgttgtgag tttcagcgat ttctgaaagt
     4381 gtatcacctt ctttaacagt gtaagttgat gattcttgag cagatacaaa tgatggagca
     4441 aatactgcaa acaaggcagc tactcctgca agagttgctt taatcttttt agttattgat
     4501 ttcatattcg aaaattctcc ttctttctat agttctatca taccctttaa atattaccgt
     4561 ttcttgacac ttttatgtag aaatattaca aaattatttt ttatttccct aaataaggtg
     4621 ttttttgtca taaaagatac tatttttatg ctatttgagg tattagaaag gttttcatcc
     4681 acaattaaag ccaagcccct cattctttga tataatagag ataagaattt ttataagggg
     4741 aaactgatga aatcacttcg ttttcaatct gtctttgata tcatcggacc agttatgatt
     4801 ggcccatcta gtagccatac cgctggtgct gttcgtattg ggaagattgt ctcttccatt
     4861 tttgatgata ctccgacaga agttgaattc caacttttta actcatttgc caagacctat
     4921 cgtggtcacg gaacagacct agcccttgtt gcaggtattt taggaatgga tacagatgat
     4981 cctgaaattc caaatagtct ggaaattgcc cacaagcgtg gtatcaagat tgtctggacc
     5041 attcagaaag acagcaatgc ccctcaccct aacaccacta aaattactgt taaaaatgcc
     5101 cacaagacca tcagcgtgac tggtatttct atcggtggag gaaatattca agtaaccgaa
     5161 ctcaatggtt ttgccgtctc tctcaatatg aatacaccga ctatcatcat cgttcatcaa
     5221 gatattccag gtatgattgc ccttgttaca gaggcgcttt cccgctatgg tatcaatatc
     5281 gcccagatga atgtcactcg tgaaaaagct ggtgaaaaag ccattatgat tatcgaagtt
     5341 gacagtcgca actgtgatga ggctatcgaa gaaattcgaa aaatccctca tctccacaat
     5401 gtcaatttct ttaaatagga ggaagcatgt tttattctat caaagaattg gtcgagcaag
     5461 cagatctgga ctttcaagga aatgtcgcag aactcatgat tacaacagag tttgaattga
     5521 ccggtcgcga acgtgaagaa gtcttccttc tcatggaacg caatctggaa gttatgaaag
     5581 cctctgtcca acttggcctc aatgaaaata aatctcgtag tggcctgaca ggtggagatg
     5641 ctgccaaatt ggaccactac atcaaaaacg aaaaaacttt atcagattac acgattctct
     5701 ctgctgcccg aaatgccatc gcagtcaatg aacacaatgc caaaatgggc ttggtctgtg
     5761 ccactccaac cgctggaagt gctggctgtc tgccatccgt tctcactgct gctattgaaa
     5821 aattagacct cagccacgag caacaattgg atttcctctt tgctgctggt gcctttggac
     5881 tagtcatcgc aaacaatgcc tccatctcag gtgctgaggg tgggtgtcaa gctgaagttg
     5941 gttcagcctc tgctatgagt gctgccgcct tgactctggc tgcaggtgga acaccttatc
     6001 aggccagtca agctattgcc tttgtcatta aaaatatgct aggcctcatc tgtgaccctg
     6061 ttgcaggttt ggtcgaagtt ccctgtgtca aacgtaatgc catgggagct agctttgctt
     6121 tcatcgcagc agacatggcc ttggcaggta tcgaatctaa aatccctgtg gatgaagtga
     6181 tcgatgccat gtaccaagta ggagcaagca tgccaactgc ctttcgtgaa acagctgaag
     6241 gtggactcgc taccacccct actggtcgtc gcctccaaaa agaaattttc ggagaataag
     6301 cttatcaaat aggagaaacc atgacctcta tcacagcgat ttttttcgat ttggatggaa
     6361 ccctcgttga tagttctatc gggattcaca atgcctttac ctataccttt aaagagctgg
     6421 gggtgcctag ccctgatgcc aaaactattc gtggttttat gggaccacct ctcgaaagta
     6481 gttttgcgac ctgcctgtcc aaagaccaaa tttctgaagc cgtgcagata tatcgttctt
     6541 actataaggc aaaaggcatc tatgaagctc aactctttcc tcagattata gacttgcttg
     6601 aggagttatc gagcagttat ccactctaca tcaccacgac aaaggataca tctactgctc
     6661 aagacatggc aaaaaacttg gaaatccatc atttctttga tggcatttat ggttctagcc
     6721 ctgaagcacc ccataaggca gatgtcattc accaagcctt gcagacacat caactagcac
     6781 cagaacaagc catcatcatc ggagatacca agtttgatat gctgggagct cgagaaacag
     6841 gtattcagaa attggccatc acttggggat ttggagagca agcagatcta ctaaactatc
     6901 aacctgatta tatcgctcac aaacccttag aagttttggc gtattttcaa tagcatagac
     6961 tgggtaacaa ttccatttca gaagaaaatg aagtaatact caatgaaaat caaagagcaa
     7021 actagtaaac tagctgcggg ctgctctaaa cactgtttta aggttgtgga tagaactgac
     7081 gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
     7141 atacggcaag gcgacgttga cgtgatttga agagattttc gaagagtata agtctctaca
     7201 taataaaacg catattacca aggttgttta acttgataat atgcgttttt cttttttatt
     7261 caatgtgggt tgtttgatta gcaaaggcga taatagcttg cttcaactca tctccactga
     7321 gagtgcggaa ctctccaatc ttttgattga tggattctaa aggcatgaca ttaaccttac
     7381 caacgaaaat cttatccata acttgattgt caacgagttc ggttgatacc aagagttctt
     7441 cgtagagttt ttcacctggg cggattccaa cttcaacgat tggaatttca ctttcagtgt
     7501 ggccacttag aagcaccatc ttcttggcca agtcataaat cttgactggt ttacccatat
     7561 caaggataaa gacttcccca tctttggcat aagcaccagc atggataacc agacggctag
     7621 cttctggaat ggtcataaag taacgggtca tgcggaagtc tgtcaccgtt acaggcccac
     7681 cttcagcaat ttgacgttca aagactggaa tgacactacc acggctacca agaacattcc
     7741 caaaacgaac tgcacagtag gttgattggc tacgttggtt aaagccagtg acaatcaact
     7801 ccgccacgcg cttggttgct cccataacat ttggtggatt gactgcctta tctgtcgaaa
     7861 tcataaccat cttaggcacc ttggcttcat caacggctct agcaacattg taagttccac
     7921 ggatattgtt tttgaaggct tcttttggat tgcgctccat cataggaaca tgcttgtggg
     7981 ctgccgcatg ataaacaata gcaggtttgt actgctcaaa gacttgcaac aaacgatcat
     8041 agtcttgaat gtccgcaatc acaggtacat aatcaatccc ttggaactta cgaatcaatt
     8101 catgataaac aaggtagatt gagttttccc catgaccgag caagacaatg cgttcaggat
     8161 tgaagcgact aacttgacga cagatttcag aaccgattga acctccagct cctgtgacta
     8221 agatggtctt acctgtcagt tctgcaccca gacgcgattc gtcaagacgg atttcctgac
     8281 gacccaaaag gtccgtaata tcaatttttt ggaagccagt acctgcttgg tgaaggccct
     8341 gaacaacagt ttcaacctta ggcatcttgt aacatttgac acccagctta ttacacatct
     8401 gcaagatacg ctcatattct gacggatcca gcgacggaat cgcaacgatg acacgctcga
     8461 tttgatggcg tttggctaat tcaggcagat tgtcataaga gcccaaaaca ggaataccac
     8521 caagtttttg accctttttc ttagaatcct tatccaaaat accgaccagt tctaattcac
     8581 tggttggatg ttggtaacta tccataaaaa gagccccacc atcaccggca ccaatcaaga
     8641 aggtccgacg gtgttctcca tcaccactac cttttttgcg tctggagtag attaactgcc
     8701 aagtaatccg tggcaataaa atcaagaagg tactcaacaa gataaagaga atgatgaaac
     8761 ggatggagaa gagtggcaag aaggcataac agatactata tgacaagaca ctgctagcag
     8821 tcacaccaaa aaagattttc atgaaatccg taatcttgct gtaacgacta atgctcgcgt
     8881 tcaaccccca aaaaccaatc atcaattgat agaacaagaa ggccaaactc gtatagataa
     8941 tgtagtcaac aggtgctgga ttaatcagcc cataaaataa aatataagat acaattatgg
     9001 aaaccaccat actgaaaata tcaaatattc cccagaaaac ctgcttttgt tgtttattta
     9061 aaatttccac cagatcaatc acataatctg ttagtttttt attcatagaa atctactcct
     9121 ccttaaaaga gttttactat attgttatta tagcatattt ttatttattt ggcttatttt
     9181 atccaaataa attattgttc tcttgtactc cttcttccat ttaaatcctc taattaaacc
     9241 tacaatcgtc ccaaggccat aagcaaagtg aatggaaaat aaaagaaagg gcatcacaat
     9301 tagaaatcca tttttatgtt ttaataaagt cagcaaagtg agtaatgaca aaagtagaaa
     9361 ataggcacct aatagtaaag ttatgaatac gaatgtgatc ggtaacaatg ctagactaaa
     9421 cacaagactc aaaacaaata aacaaggaac atagtgaaat aatgataaac acttaaactg
     9481 aacatgactt gtcaagccaa tccacaaacc atttgaatac ttttgatgca gcattttctt
     9541 gaatgttggt cgaatatact gataagatag aatacttggg ctatagcgga ttttataacc
     9601 atgttctcga attctataat gaatatcatt atcttcagtt cggccaagtt gctcatttac
     9661 taaaccaacc ttctggaaaa cctctcgttt atacatccca tgaaaaatag aagaaacata
     9721 tctatcctcg gaactatttc gataattggc aatgctactg ccaaacatat tttcctcaac
     9781 aagatgcaag gtctctgccc attttccttt tccttcgaca atcgtcggtc taggcccccc
     9841 acagacaaat tcaccttgtt gaataatagc cacattgttc attacaaaac tctcagtaac
     9901 ttttgaatga gcatcaattt ttaaaataag gtcccctaca gaatgtttaa ctcccaggtt
     9961 aaaaccacta gcttgatttt tcttaggatt gttatacaat ctaattgagt taaactctgt
    10021 atcttccttt ataaattgct gaatgatagc tgtggtccca tctgtggaca tagcatttat
    10081 aaatagaatt tcaatattct ctttaggata ggtttgattt tttaagtctt caattagacc
    10141 aggcagatat ttttcttcat tgtatgcact gattacaatg gatacgagaa gactttcaac
    10201 attttgagtc actactactc tcctgaattt ttccttccac cattctatca taattttcaa
    10261 gactttccca tttttatctt gaaagccaga aaccttactt gacattatag tgaaaaagcc
    10321 ttaaaataag ctgttattaa aatcagctag aagaggtatt atatgaaaaa gcaatcactc
    10381 ttttttgttc caggtattat cctgattggt gtttccttgc gaactccttt tactgtttta
    10441 cccattattt tgggaaatat ttcgcaaggt ctggaggtag aagttagttc gcttggtgtc
    10501 ttgaccagcc tgcctctcct tatgtttacc ctcttttcac cattttctac ccaactggct
    10561 cagaaaatcg gcttggagca tctcttcacc tacagcctct tcttcttgac catcggctca
    10621 cttattcgac taatcaatct gcccctgctc tatctaggaa ccttgatggt tggggcaagt
    10681 gtcgcagtca tcaatgtcct gcttcctagt cttatccaag ccaatcaacc aaagaaaatt
    10741 ggttttctga ccaccttata tgtaacgtct atggggattg caacggctct ggcttcctat
    10801 ctagctgtgc ccattacaca agccagttct tggaaaggac ttatccttct cctcacgtta
    10861 ctctgtctag caactttttt ggtctggctc ccaaatcacc gctataatca tagactagct
    10921 ccacaaacca aacaaaaaag tcaaataaag gtcatgcgta ataaacaggt ttgggcaatt
    10981 attatctttt caggttttca atccttgatc ttttacaccg tcatgacctg gttacctacc
    11041 atgtctatcc atgcaggtct atccagtcac gaagctggct tgctgacttc tatcttatct
    11101 ctgattagca ttcctttttc aatgaccatc ccaagcctga caaccagttt atctactcgc
    11161 aaccgtcagc tcatgctcac tttggtttca ctagctggtg tggtcggcat ttccatgctc
    11221 tttttcccaa tcaataattt catttactgg cttaccatcc atctcctcat cggaaccgca
    11281 accagcgccc tcttccctta tctcatggtc aacttttcac tcaagacaag cgcccctgaa
    11341 aagacagccc aattgtccgg cctatctcaa acaggaggct atatcctagc agcctttggg
    11401 ccaaccctct ttggttacag ttttgacctg ttccactctt gggtaccagc tgtagctgcc
    11461 ctcttgctca tctatatcct gatgactgtg gccctcttta cagtggacag agcggataag
    11521 atcctttaaa cttctagttt tagctagaag ttttttatat ataaaaattt tacacatttc
    11581 tcttgacagg gctttctttt agatgtacaa tgtatgtgta gaaaaattat atataaaaat
    11641 cttacacatt agaaaaggag gtttcccatg tactttccaa catcctctgc cttgattgaa
    11701 tttctcatct tggctgtact ggagcagggt gattcttatg gttatgagat tagccaaacc
    11761 attaagctga tcgctaatat caaagaatcc acactctatc ccattctcaa aaaattggaa
    11821 ggcaatagct ttctgacaac ctattctaga gagttccaag gtcgcatgcg caaatactac
    11881 tccttgacaa acggtggtat agagcagctc ttgaccctaa aagatgaatg gacactctat
    11941 acagacacca tcaatggcat catagaaggg agtatccgcc atgacaagaa ctgaatacct
    12001 gactcagcta gaactctatc tcaagaaact acctgaagct gaccgtatcg aagccatgga
    12061 ctatttcaga gagctctttg acgatgctgg agtcgaagga gaagaagaac tcatcgctag
    12121 tttgggaact cccaaagaag cggcccacga agttctatcc aatcttctcg ataaaaaaat
    12181 caatgaagca cccgctcaaa aaaataaccg acaaatttta catatcgcct tgttagccct
    12241 ccttgcagca cctatcggca ttcctctggg aatcgccatc ctcgtgaccc tgttcgcaat
    12301 ccttgtagcc gctttgactg tcattctggc tttctttgca gtttccatac tgggtatcat
    12361 cggcggattc ctatttttag ttgaaagttt cactatcctc gcccaagcca aatcagcctt
    12421 tatcttgatt tttggttctg gtttactggc tatcggtgct tcttcgctag ttttacttgg
    12481 catttcctat gtagctcgct tcttcggtct actcattgtt cgtctggtac aatttgttct
    12541 taaaaaagga aagagaggta atcagcatgc gtaaatggac aaaaggattt ctcatctttg
    12601 gtgtggtgac taccgttatc ggctttatcc tgctttttgt aggtatccaa tctgacggga
    12661 ttaagagcct actttccatg tccaaagaac ctgtctatga tagccgtacg gaaaagctaa
    12721 cctttggcaa ggaagtcgaa aacctagaaa ttactctcca ccaacacgcg ctcaccatca
    12781 cagactcttt cgatgatcaa atccacattt cttaccatcc atctctttct gctcaccatg
    12841 attttatcac caatcagaac gatagaactc tgagtctcac tgataagaaa ctgtctgaaa
    12901 ctccgtttct ctcttctgga attggtggga ttcttcatat cgcaagtagc tactctagtc
    12961 gttttgaaga agttattctc cagctaccaa aagggagaac tctaaaaggg atcaacatct
    13021 cagccaatcg cggacaaacc accatcataa atgctagcct tgaaaatgcg accctcaata
    13081 caaacagcta tatcctccga attgaaggaa gtcgtatcaa aaacagtaaa ctcacaacgc
    13141 ccaatatcgt taatatcttt gatacagttc ttacagatag tcagctagag tcaacagaga
    13201 atcacttcca cgctgaaaat atccaagtcc atggcaaggt tgaactgact gccaaagatt
    13261 atctcagaat catcctagac cagaaagaaa gccaacgaat taactgggac atctcaagca
    13321 actatggttc tatcttccaa ttcacaagag aaaagcctga atcaagaggt acggaattaa
    13381 gcaaccctta caaaactgaa aaaaccgatg tcaaggatca actcattgcg agatctgatg
    13441 ataatattga tctaatatcc acaccaagca gacgttgaca aaaacgctta catctgctgc
    13501 tatggaagca tatttatcta accaaaagga ggttctacca tgaaacaaga atggtttgaa
    13561 agtaatgatt ttgtaaaaac aacaagcaag aacaagcctg aagagcaagc tcaagaggtt
    13621 gcagacaagg ctgaagaaac gatagccgat ctcgatacac caattgaaaa aaatactcag
    13681 ttagaggagg aagtctctca agctgaagtc gaattggaaa gccagcaaga agagaaaatt
    13741 gaagctcctg aagacagtga agcgagaaca gaaatagaag aaaagaaggc atctaattct
    13801 actgaagaag agccagacct ttctaaagaa acagaaaaag tcactatagc tgaagagagc
    13861 caagaagctc ttcctcagca aaaagcaacc acgaaagagc cacttcttat cagtaaatct
    13921 ttagaaagtc cttatatccc cgaccaagct ccaaaatcta gggataaatg gaaagagcaa
    13981 gtgcttgatt tttggtcttg gctagtggaa gcgatcaaat ctcctacaag taagttggaa
    14041 acaagtatca cacacagtta cacagccttt ctcttgctca ttctgttttc tgcatcttcc
    14101 tttttcttta gtatctatca catcaaacat gcttactatg gacatatagc aagcattaac
    14161 agtcgcttcc ctgagcagct agctccttta actctttttt ctatcatctc tatcctagta
    14221 gcgacaacac tcttcttctt ttcattcctc ttgggtagtt tcgttgtgag acgatttatc
    14281 caccaggaaa aggactggac gctagacaag gttctccaac aatatagtca actcttggca
    14341 attccaatct tcctcactgc tattgctagt ttctttgctt tctttgatag cctacgattt
    14401 acagccctct tgtgtgtgat tagcattgga atcattctgc ttgccagtct ccatatcatt
    14461 acaagaccaa gtcaagcaag tgaaaccgac tccttctatc aattattctt gtctgtcctt
    14521 gtgaacggag ttattatcct cctcttcttt gtagctgaag tggcactgat tggagattat
    14581 cttcgtatct tggcctttct ttaaaattca atcctgtcat ccttggcagg attttttcta
    14641 tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact tttcttgtgc taatgtttta
    14701 aaatcttgtc ctaggatggg ttgtatttct aattgattgg cgtctagttg gtggtaaaaa
    14761 gataggggta acacctctaa gaattttgca tagtccaagc gagctatgga ttcatagtct
    14821 tctagtttag agccgtctcc tgtgatttga accaagtcaa tctcccactg gacttcctta
    14881 tccaccaatt gctcaatata ggctgcagga acaaacggtt ctctcggtaa aactgtcttg
    14941 actccttgag ctagatggta aacgccatgc acttttcctt ctccatcctg atagaaggaa
    15001 actctcgcaa agtaggcatc tgtctcttga accgcacgca cacgcgcttc aaactgagcc
    15061 aagccatctg cctctaaaaa gcttttcaag ttctcacgac taaagaaaat aggattaaaa
    15121 attccttggc aaatcgtaaa acgagctaca gggtctgcta gataggcttg aatacttgct
    15181 tggaaataag cttcaaaatc aaaaccatct agcccttcaa tatcagtccc tgtataagca
    15241 agcaaagcat ctaaaaatga cttgataatc tgccagtctg tctttgttag taggtcagga
    15301 agatcgacac ggtaagcctt ttgaccatca gcataactga ccttaaagag aaattgtgat
    15361 tgccccacta tcgcacactc gatatattcg agacgattaa gaggctgacg gagatagact
    15421 acatcatagc tatgtgactc caaaccgtct agcaaggcca agatcgactt ggcattcaaa
    15481 atctcttgtt ctcctaaaat gctctgttta tttggaataa aaaatgtttt cgccataact
    15541 ggactccttt gaaatcgttt acatacagta taccttattt ttctgaaaga tacaaaaaca
    15601 aactttatac tcttcgaaaa tctcttcaaa ccgcgtcagc ttcgccttgc cgtagatata
    15661 tgtaactgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag cagcctgcag
    15721 ctagcttcct agtttgctct ttgattttca ttgagtatta gatttttgag tacaaagcat
    15781 agaaaaacag cccctgagga ctgtttgatc ttatacagta gctgcttgat ccaagctttc
    15841 accgatagcg gctaggcgct cgataacttc agcttgtgtc aattcatttt ttgaaacata
    15901 gcggttacgt gggtgaacac ggcactcgtg tgagcatcca cgaaggtact tgtcttcatt
    15961 ttcttctgat gtcaagatac gacggttaca gaagggattt ccacagttga cataacgttc
    16021 acatggtgtt ccatcaaacc agtctttccc tacgatagtt gggttgacat ggttgacatc
    16081 aacggcaata cgctcgtcaa agacgtacat tttcccatcc caaagctcac cttgaacttc
    16141 tgggtcttta ccgtaagttg cgattcctcc gtgcaattgg ccgacatctt tgtagccttc
    16201 acggaccatc cagcctgaga atttctcaca gcgaacgcca cctgtacagt aaaccacgac
    16261 acgcttgtcc atgaattttt ctttgttgtc acgaacccat tgtggtaact cacggaagtt
    16321 gcgaatatct gggcgaatag ctccacggaa atgtcctagg tcgtactcat aatcgttacg
    16381 tgtgtcaagg acaacggtat ctttatcaag aagcgcttct ttgaactctt ttggagacaa
    16441 gtaagcacct gttgtttcaa gtgggttgat gtcattgtca aagtcgttgt cttccaaacc
    16501 aaggtggaca atttctttct tgtagcgaac aaacatcttc ttgaaggctt gttcattttc
    16561 ttcgtcaatc ttgaaccaga gttcttccat acctgggagg ctgtgaacgt agtccatgta
    16621 tttttgagtt gtttcatagt cacctgaaac tgttccgtta attccctcgt cagcgactag
    16681 gatacggcct ttaaggccga ttgatttaca gaaagccaag tggtctgcag caaattgctc
    16741 tgcattttca attggagtat aaaggtagta aagtaagaca cgaatatctt ttgccataag
    16801 atttgttctc ttttctattc ttaaattttc taaatttttt atttatccat actagtatac
    16861 ctgcttgaga aaacgaatgc aatttatttg actggaaatt gtagaggatt ctcgaaattt
    16921 cctgaatggt taaaataagg acaagagaaa acagggatat ctatatcctt gtgccaaaaa
    16981 aaccactgcc ctccccagac caacctgagg aaagcagtga ttcttatttt aggagttagg
    17041 aatgaataca cgaaatcaat ttagctgatt attttttgtt tttcaagaat tcatcgtatt
    17101 gtttttgcat ttcgttcaat actttttcgt aggcaccttc agatttcaat ttttccatca
    17161 attctggaat cgctttatct gggtctacag taccagtgtt gatagctgta tcaaattgtt
    17221 gcattgtgtt agcaatagct gagatttcag atttcacatt gtcagtattg aagataaatc
    17281 caagcgctgg agattcttta gcttctgcca attctttctt agaattttcg atttgttggt
    17341 ctgtaacgtt ttcgttgatg taaaggatcc agttgttacc agtgttccat ccacccatgt
    17401 gagtgtttcc tttgtagcca tcaagaacgc gaacacggtt ttctttacct tcaatttttt
    17461 cccagttctt gccttctgga ccgtaaacaa gaccgttcaa gagttctgga ttcgtattca
    17521 agaggttcaa gatttccatt gatttttctt tgttcttaga gttgtttgag atgacaaagt
    17581 tagcaacttg tgttgtttgg tttttcttga tgaagttagt aattggtttg atttggatat
    17641 ctttgttggc aacacgtgaa agcaagctgt taccgtagtc agctggtcct actgtttctt
    17701 cacgaacgaa ccaagtatct tgttgaaggt caaaggaagt atcgcttgtt gcgacgtctt
    17761 ttggaatgta gccagcttca tagaatttgt gaagagtctt caagtgttct ttgaaacgag
    17821 gcacttcgta acggtttaca actttagtag tatcgccttc aaggtcaata acgaatggaa
    17881 gcccgtttgc tactgggtag tcaaaattat cagatgggat gaaaacttta ccaatagcaa
    17941 atggtactac gtctggagct ttttctttga tttgtttcaa gactggctca agagtttcgt
    18001 aagaagtaac acctgaaata tcgataccat atttggcaag gagagttccg ttgaaggcaa
    18061 agttttgaga tgatgcaacg ttggctgcaa ctggtacagc ataaatctta ccatttacag
    18121 tattaccctt gatataagct gggtcaagtg ccttgtaaag gtctttacct tcttttttgt
    18181 acaattctgt caagtcagca taagcacctt tttgagcatt tacaatatag ttatctgcaa
    18241 aggcgatatc atagttttca ccagatgatg tgataactga cattttctta ccatagtcac
    18301 cccagccaag gtattggata tccaatttag caccaacttt ttcttcgatg attttgttgg
    18361 catttgctaa caattcatcc aagttatctg gtttgtcacc gatttggtac attttgataa
    18421 caggtttgtc acctgaatca gcagcttttt tactgttacc tgtcaagttg ccacaagcag
    18481 caagacctgc agccaaagcg actacactag cagatgcaaa agcatatttt ttccagtttt
    18541 tcatgataaa aactcctttt tttattttta aacttataaa caatgtaatg atcttaactt
    18601 cacgcaaggg aagtttggaa atgagaaatg ttctttttca ataagcacta ttctttcaca
    18661 ccaccgatag tcaaaccttt tacaaagtag cgttggaaga atggatacaa aatcgcgatt
    18721 ggaagggttg ctaccacaac catggccata cgacctgttt ctttcggtag agcaactccc
    18781 agttgaccag tcaagccgac cgctttggca atgtagtcca tattttgttg gatttgcatg
    18841 agcaaatatt gcaatggata caagttatca ctcttgatgt aaagaagggc gttgaaccag
    18901 tcattccaga aaccaagagc tgtcaaaagc gtgatggttg cgatacctgg tagtgacaat
    18961 ggcaaacaga tttggaagaa aatccgagcc tcactggcac catcgatacg agccgattcg
    19021 agaatggctt ctggaatggt cttcttgaaa aaagaacgca tcaagatgat attgaatggt
    19081 gagagaagca ttggaacaat caaggcccaa acagtgtcac caagctgaag tacacgggtc
    19141 accatgatat aacctggtac caaaccagcg ttgaacaaca tactgagaag gacgaagatg
    19201 gtaaaaaatc tgcgatactt aaaggttgtc cgtgaaatag cgtaggcata ggttgttgtg
    19261 ataaagacat ttgtcaatgt cccaactacg gttacaaaga cagagatgaa gagggcttgt
    19321 aggattttat ccttaaactg tgccaaaaac tcaaaaccgt ctaagccaaa ttgggatggg
    19381 aagaagctat agccgtattg gaggaggctt ttctcgtctg tcactgaaat aatgataacg
    19441 aatacaaaag gtaggataca agagagggca atcaaacccg aaataatact gaagaagata
    19501 tctgctttct tactgaagga gtgaatgccg acattatcaa ttttttcttt tttaattttc
    19561 ttttttgcca tattctcctc ctttctagaa caaagctgag tttggatcga ctcgtcttgc
    19621 aagcaagttt gataggataa ccagaatcaa accaacaacg gattggtaaa gaccggctgc
    19681 tgcagccata ccgatatctg ctgtctgagt caaaccatta aagacatata cgtccaaaac
    19741 gttggttaca ttgtaaagct gaccagcatt gtgtgggatt tgatagaaga gaccgaagtc
    19801 tgcgcggaag atatttccga ctgcaaggat ggtcaataca gttacaagcg gagtcaactg
    19861 aggaatggtt acgttgcgaa tacgttgcca cttgctagct ccgtccactg tcgctgcttc
    19921 gtagtaggtt ggatcaattc ccatgatcgt cgcatagtac atgacactgc tatatccaaa
    19981 gcctttccaa atacctagga aaagtaggag atagggccag atgcccaggt cagcgtagaa
    20041 attgacttct ttgagaccaa gactttccaa tagatgattg aacacccctt tatcaatatt
    20101 taggaaggca tctgtaaaga aactaatgat aacccaagac aagaagtaag ggaacaacat
    20161 agaagtttga aaaatcttca ccattctctt agaacggagc tcgctgagga taatggcaat
    20221 ccctacagat acaactaaac ctagaaagat aaagccaaga ttgtagagga cagtatttcg
    20281 tgtgataata aaggcgtctc ttgaactaaa taagaatctg aaattatcga gtccgaccca
    20341 tttactattt atgatactat ctatgaaacc attactggtc atgtggtagt ctttgaaggc
    20401 aaccacgttc ccaaatactg gaatgtaaaa gaatagaatc aaccagagtg cccctggcaa
    20461 aaccatcaag agaaagatcc agttgtctct caatgttttt gaaaactttt tcataatttc
    20521 ctcccttttt attttgatat ccatctaaaa attctttttt agacttttga taacgattac
    20581 attattagta tactcctatt tgcaggttag gttaaactcc taattataga aaaaactcca
    20641 caaattatgt agcagattta aaactttatc accactatca aacaaatgtc ctaaatcaat
    20701 tgtttatttt atctctatta gcccagtgat ggcgtcactc tgttataagc atccaacaac
    20761 ggggtatact gaaaaatctc cagactaggg aactcagaga tagttcctaa tctggagatt
    20821 tttaatatgt tattaggcgt ttgctttcaa cttagcaata acctctttaa gattatcaat
    20881 cacctctgct tcagtatgct cagagccttt ttcatctgcc aagaacaaaa ctgctttttg
    20941 aagttctttt tgagagtttt caaggacatc cttatctact gtttcaaggt ttgagtcttt
    21001 aagaagttta cttaattcct tggctaattt cttgagtttg atttgcagac tcatcttctc
    21061 ctgctgtttc tttgcccgct gtttgtcctc catccttagt tgctgactgg ctttccttaa
    21121 tggactctag ggaagcaatg gcatctttga ctgtttgcaa gatatcacgt aaaccttgct
    21181 ctgtcaaact atcatctgca aaagctttct tagcctctgc caaaaccaga cgtgctgaat
    21241 ctgtggtagg attcgataca cctgtcaatg atctcaaaag attttctaag gtttgagtct
    21301 gcttactaat actagactaa aatcaaaaag cattatataa cagtgatatg aaatcaacta
    21361 aagaagaaat ccaaactatc aaaacacttt taaaagactc tcgtacagct aaatatcata
    21421 aacgccttca aatcgttcta tagtaaaatg aaacaaggac agtcaaatcg atttctaaca
    21481 atgttttaga agtagaggtg tactattttt ggttcatttt actatatcac tttgaactat
    21541 atcggtctag ttatgtctaa acgattatag ctggatacta agagaaatca aaaaagcact
    21601 aagggagaac gtcccaaagt gcttaatatc aaggcctcaa agcctatctt attcaataaa
    21661 atattacaac attttgttgt agaattcaac gacaagtgct tcgttgattt ctgggttgat
    21721 ttcgtcgcgt tctggcaagc gagtcaatga accttccaat ttttcagcgt cgaatgatac
    21781 gaatgctgga cgtccaagag tagcttctac tgcttcaagg attgctggaa ctttcaatga
    21841 tttttcacga actgagatca cttgacctgg agttacgcgg tatgatggga tatcaacgcg
    21901 tttcccgtca acaaggatgt gaccgtggtt tacgaattga cgagcttgac gacgagtagt
    21961 cgcgagacca agacggtaaa caacattatc caaacgacgt tccaaaagaa gcataaagtt
    22021 gaaacctagg attccacctt tgatttttgt agcttgtacg aacaagttac ggaattgttt
    22081 ttcacctaca ccgtaagtga aacgaagttt ttgtttttca gccaattgca aaccgtattc
    22141 tgacaattta gaacggttgt ttggtccgtg ttgtcctggt acgtagttac gacgtgccaa
    22201 ttctttacct gtacctgtaa gtgaaaggcc aaggcgacga gcttgtttcc aagatggtcc
    22261 tgtataacgt gacatatgaa tgtcctcctg atataaataa tatttcggtg gaaatagtca
    22321 cttagaaagc cctgattcgt gcagatgccc ttcgcctaaa cagccaaggt tacttgtcat
    22381 aagacacctg ttgacgagct tcatgctttc ctgctgatat ttcacacaaa atccatttta
    22441 ccatgaatag ctggatttgt aaaggggttt taggctttat tttcactacc agagaggttg
    22501 agaacgaggg taaaggtact tcctagaccg tactggctgc tgactgtgat ttccccaccc
    22561 aattgatggg ccaattcacg cgcaatcgca agtcctaatc catgaccacc tgtcttcatg
    22621 ttacgcgaag tttcgacacg ataaaggcgt ttgaaaatat tttccaaatc ctctggggca
    22681 atgccctgcc cttcatcggt cacactgatt gaaagctggt ccttctccag cttagccacc
    22741 acttccagct tggttcctgg agcagaatat ttaaaagcgt tatcgaccag attcaccaag
    22801 atacgagaaa gcttagcata atctccctca atccgggcag actctgggat tacctgcaag
    22861 tggacatctc ttctctcctg ctcaatcaaa aactgaaatt cactcatgca ctcaattaag
    22921 agcttgtcca gaaaaatact gtctttactg gtagtttcca cctgatttct agctgtgttt
    22981 agggtcaaaa aattcaactc ctcaaccagt ttattgagcc tctccgtctg gcgtccaatg
    23041 gttgctagat aatgagcttg ctccgactcc ttgataatcc catccaaaat cccttctacc
    23101 gtcgcttgaa tcgaagtgat aggggtctta atatcatgcg acaactgggc aatcatcaag
    23161 cccttttctc gttcgctttc ttccaaggaa tcaaagctta cctgcaaatc atgggacatc
    23221 tcattaaaag tttgccctaa ttgctgaaat tctacaggac cttgaacctc caaatttgaa
    23281 ggaaaatcct tggccgctac ccgcttggca tgctccttga gtttgcccaa cgacgtaaag
    23341 actggcaata ggagaaagag actaatccca gcaccgacaa ggctggcaac gatggtcatc
    23401 ccaagcaaaa agtaaatctc gcctttcgca atcagcattt tttgaacagc ccaaaaaaca
    23461 accaaaatgg ttaagagggt tgaaataata tatccaacca aaatataact ttttagtttc
    23521 atgtttgtcc tctcggtttc tctatcttat atcccaaccc ccaaactgtc ttaatagtgg
    23581 gcgtttggtc actactatat tttgccagct cctgtcgaag agcatggata tgcacattca
    23641 aggtattggt gtcatccacg tagtcttctt tccagatctt ttcatagagg tctgtcttgg
    23701 agaaaactcg ctctggattg ctagctaaaa tccacagcaa ttcaaatgat ttaacagtta
    23761 aatccagcat ttcttctcct atttgaactt catgactact atgattcatt tttagattgc
    23821 caagggaaat cagctctgtt tcgcccccac gatgaaggcg gcgcaaaata ttgtggacac
    23881 gcaaaactag ctcacgtggg ctaaaaggct tagcaataaa atcatctgct cccaagctca
    23941 ggccgtaaat cttgtcctgt tcactggtct tagcagtaat aaataggaaa ggctgctctg
    24001 gtgataagta ttgaacctca ctgattaaat cataaccatc catccgaggc atcatgacat
    24061 ctgtgataat caaatcaatc ggttttttct tgaacagctc tagcgcttcc agtccatcat
    24121 gggctaccaa gacctgataa cctgcctgaa ttaagtatct ctgatgaata tctgtgattt
    24181 ctacctcgtc gtcaacgagt aaaattgtct ttcccatctg tctctccttt gataaaaaca
    24241 gtgctatact gctttaagta taacactatt tttattgttt tacgatgatt ttaatctgct
    24301 attgaaagaa agtcctgatt gctgattgag ttgttaaact aatcttagtc agcccactat
    24361 ccatcattta tttccttagc tgattagttt tcttcttttt tgtttttcaa acctagacca
    24421 ccgagtaaac ctgcaagcgc aagcccaagg aaaccaatac ttgccattga tgtttgagtc
    24481 tcaccagtat ttggtagcat agctttatcc tctgacatca tcgtatcaga catcttgtta
    24541 gcagaagcag ccatgttttc acctgccatc gtgttggtag aacttgtcat ggtgtcagca
    24601 ggcatgctat ctgtagtacc tgtagcatga ttgtgattca tcggagtcac gccagaacca
    24661 gagttagaag gtgataatga accatttgct gtgtctgatg tttctttaac atttatctta
    24721 atagtgactt ttttagttgc tacgatgttg tccaagtctg gtttaccgtc tttgttacca
    24781 tagacattga ctgtagcgct gtaagtttga gtaccgtttg ctcggaactg gtcaatgagc
    24841 gcttgttttt ctttgccagc tacatttccg tccaaggcta cttgatagaa gtattgacct
    24901 ttggtcttca cgttttcgcc tagtggagat agggctgggt ttttagcctc
//
LOCUS       DAVKJU010000026        24826 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVKJU010000026 DAVKJU010000000
VERSION     DAVKJU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.026"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            186..416
                     /gene="blpU"
                     /locus_tag="VW191_001033"
     CDS             186..416
                     /gene="blpU"
                     /locus_tag="VW191_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEU9623816.1"
                     /translation="MNTKKMSQFEIMDTDMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
     gene            1131..3284
                     /locus_tag="VW191_001034"
     CDS             1131..3284
                     /locus_tag="VW191_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9623817.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMAFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGSVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            3297..4646
                     /locus_tag="VW191_001035"
     CDS             3297..4646
                     /locus_tag="VW191_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU9623818.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4816..5523
                     /locus_tag="VW191_001036"
     CDS             4816..5523
                     /locus_tag="VW191_001036"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU9623819.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5725..9450
                     /locus_tag="VW191_001037"
     CDS             5725..9450
                     /locus_tag="VW191_001037"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEU9623820.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELKAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEAIQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKVG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            9543..10985
                     /locus_tag="VW191_001038"
     CDS             9543..10985
                     /locus_tag="VW191_001038"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU9623821.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFFFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            11022..12044
                     /locus_tag="VW191_001039"
     CDS             11022..12044
                     /locus_tag="VW191_001039"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU9623822.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYIKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            12041..12586
                     /gene="purN"
                     /locus_tag="VW191_001040"
     CDS             12041..12586
                     /gene="purN"
                     /locus_tag="VW191_001040"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU9623823.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12670..13179
                     /locus_tag="VW191_001041"
     CDS             12670..13179
                     /locus_tag="VW191_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEU9623824.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            13204..14751
                     /gene="purH"
                     /locus_tag="VW191_001042"
     CDS             13204..14751
                     /gene="purH"
                     /locus_tag="VW191_001042"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU9623825.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKASPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMALGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14873..16135
                     /gene="purD"
                     /locus_tag="VW191_001043"
     CDS             14873..16135
                     /gene="purD"
                     /locus_tag="VW191_001043"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU9623826.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELYQQKIEGLFYRTDIGSKAIK"
     gene            16538..17026
                     /gene="purE"
                     /locus_tag="VW191_001044"
     CDS             16538..17026
                     /gene="purE"
                     /locus_tag="VW191_001044"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEU9623827.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            17013..18104
                     /gene="purK"
                     /locus_tag="VW191_001045"
     CDS             17013..18104
                     /gene="purK"
                     /locus_tag="VW191_001045"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEU9623828.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPVADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            18114..18341
                     /locus_tag="VW191_001046"
     CDS             18114..18341
                     /locus_tag="VW191_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEU9623829.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            18404..19702
                     /gene="purB"
                     /locus_tag="VW191_001047"
     CDS             18404..19702
                     /gene="purB"
                     /locus_tag="VW191_001047"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU9623830.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19757..23695)
                     /gene="strH"
                     /locus_tag="VW191_001048"
     CDS             complement(19757..23695)
                     /gene="strH"
                     /locus_tag="VW191_001048"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9623831.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAVLQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEALKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(24010..24726)
                     /locus_tag="VW191_001049"
     CDS             complement(24010..24726)
                     /locus_tag="VW191_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9623832.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDREH"
BASE COUNT         6831 a         4861 c         5889 g         7245 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaaag atgtcacaat ttgaaattat ggatactgat atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagcacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgtgggagg agctatactt ggaggtgtgg cctatgcagc gacatgttgg tggtaattat
      421 ggattttaaa agttttatta ttggtttagt agttggtata tttggtcctt atatggatga
      481 tttaattaga aaaaaatttt taaagtcttc ggagaagaaa acagaaaaat ctgttaaaaa
      541 ataatcaaaa ctataaatga tgaatctgaa tcaaaattat tttgcgcatg taaagaggag
      601 tcttatagta acgagtcaaa aaaggagtaa ctatgaatcg taatttagaa cggtgttatc
      661 tattctgact aggaatagat cataccagag gtagcttaga aatagcagag acattagaaa
      721 ttgaagtaat aaataggatg tcgtaagtgt tactatcaat gatttatttg tttcaagctt
      781 gcctagggtg acagtaaaaa atcaatttcc tttcaatagc atatttttag tgggcaggac
      841 tcttgttctg cctatttttt tatccaaaaa gtgcagttgg gagggagata ggctcatttg
      901 ggaaggaagt ccagtttttg tttagtgatt gaggtaagat agttgttatc agatgagttt
      961 atactcttcg aaaatcaaat tcaaaccacg tcaacgtcgc cttgacgtat atatgtgact
     1021 gacttcgtca gtcctatcta caacctcaaa acagtgtttt gagcagcctg cggctagttt
     1081 cctagtttgc tctttgattt tcattgagta ttagggaaaa ggagatgaat atgaaatttg
     1141 ggaaacgtca ctatcgtccg caagtggatc agatggactg cggtgtagct tcattagcca
     1201 tggtttttgg ctactatggt agttattatt ttttggctca cttgcgagaa ttggctaaga
     1261 cgaccatgga tgggacgacg gctttgggct tggtcaaggt ggcagaggag attggttttg
     1321 agacgcgagc cattaaggcg gatatgacgc tttttgactt gccggatttg acttttcctt
     1381 ttgttgccca tgtgcttaag gaagggaaat tgctccacta ctatgtggtg actgggcagg
     1441 ataaggatag cattcatatt gccgatccag atcccggggt gaagttgact aaactgccac
     1501 gtgagcgttt tgaggaagaa tggacaggag tgactctttt tatggcacct agtccagact
     1561 ataagcctca taaggaacaa aaaaatggtc tgctctcttt tatccctata ttagtgaagc
     1621 agcgtggctt gattgccaat atcgttttgg caacactctt ggtaaccgtg attaacattg
     1681 tgggttctta ttatctgcag tctatcattg atacctatgt gccagatcag atgcgttcga
     1741 cactagggat tatttctatt gggctagtca tcgtctacat cctccagcaa atcttgtctt
     1801 acgctcagga gtatctcttg cttgttttgg ggcaacgctt gtcgattgac gtgattttgt
     1861 cctatatcaa gcatgttttt cacctcccta tgtccttctt tgcgacacgc aggacagggg
     1921 agatcgtgtc tcgttttaca gatgctaaca gtattatcga tgcgctggct tcgaccattc
     1981 tttcgatttt cctagatgtg tcaacagttg tcattatttc ccttgtttta ttttcacaaa
     2041 ataccaatct ctttttcatg actttattgg cgcttcctat ctacacagtg attatctttg
     2101 cctttatgaa gccgtttgaa aagatgaatc gggacaccat ggaagccaat gcggttctgt
     2161 cttcttctat cattgaggac atcaacggta ttgagactat caagtccttg accagtgaaa
     2221 gtcagcgtta ccaaaaaatt gacaaggaat ttgtggatta tctgaagaaa tcctttacct
     2281 atagtcgagc agagagtcag caaaaggctc tgaaaaaggt tgcccatctc ttgcttaatg
     2341 tcggcattct ctggatgggg gctgttctgg tcatggatgg caagatgagt ttggggcagt
     2401 tgattaccta taataccttg ctggtttact ttaccaatcc tttggaaaat atcatcaatc
     2461 tgcaaaccaa gcttcagaca gcgcaggttg ccaataaccg tctaaatgaa gtgtatctag
     2521 tagcttctga gtttgaggag aagaaaacag ttgaggattt gagcttgatg aagggagata
     2581 tggccttcaa gcaggttcat tacaagtatg gctatggtcg agacgtcttg tcggatatca
     2641 atttaaccgt tccccaaggg tctaaggtgg cttttgtggg gatttcaggg tcaggtaaga
     2701 cgactttggc caagatgatg gttaattttt acgacccaag tcaaggggag attagtctgg
     2761 gtagtgtcaa tctcaatcag attgataaaa aagccctgcg ccagtacatc aactatctgc
     2821 ctcaacagcc ctatgtcttt aacggaacga ttttggagaa tcttcttttg ggagccaagg
     2881 aggggacgac acaggaagat atcttacggg cggtcgaatt ggcagagatt cgagaggata
     2941 tcgagcgcat gccactgaat taccagacag aattgacttc ggatggggca gggatttcag
     3001 gtggtcaacg tcagagaatc gctttggcgc gtgctctctt gacagatgcg ccggtcttga
     3061 ttttggatga ggcgactagc agtttggata ttttgacaga gaagcggatt gtcgataatc
     3121 tcatggcttt ggacaagacc ttgattttca ttgctcaccg cttgactatt gctgagcgga
     3181 cagagaaggt agttgtcttg gatcagggca agattgtcga agaaggaaag catgctgatt
     3241 tgcttgcaca gggtggcttt tacgcccatt tggtcaatag ctagaaagag gagaggatga
     3301 aaccagaatt tttagaaagt gcggagtttt ataatcgtcg ttaccataat ttttccagta
     3361 gtgtgattgt acccatggcc cttctgctcg tgtttttact tggctttgca actgttgcag
     3421 aaaaggagat gagtttgtcc actagagcta ctgtcgaacc tagtcgtatc cttgcaaata
     3481 tccagtcaac tagcaacaat cgtattcttg tcaatcattt ggaagaaaat aagctggtta
     3541 agaaggggga tcttttggtt caataccaag aaggggcaga gggtgtccaa gcggagtcct
     3601 atgccagtca gttggacatg ctaaaggatc aaaaaaagca attggagtat ctgcaaaaga
     3661 gcctgcaaga aggggagaac cactttccag aggaggataa gtttggctac caagccacct
     3721 ttcgcgacta catcagtcaa gcaggcagtc ttagggctag tacatcgcaa caaaatgaga
     3781 ccatcgcgtc ccagaatgca gcagctagcc aaacccaatc cgaaatcggc aacctcatca
     3841 gtcaaacaga ggctaaaatt cgcgattacc agacagctaa gtcagctatt gaaacaggtg
     3901 cttccttggc cggtcagaat ctagcctact ctctttacca gtcctacaag tctcagggcg
     3961 aggaaaatcc ccaaactaag gttcaggcag ttgcacaggt tgaagcacag atttctcagt
     4021 tagaatctag tcttgctact taccgtgtcc agtatgcagg ttcaggtacc cagcaagcct
     4081 atgcgtcagg gttaagcagt caattggaat cccttaaatc ccaacacttg gcaaaggttg
     4141 gtcaggaatt gacccttcta gcccagaaaa tcttggaggc agagtcaggt aagaaggtac
     4201 agggaaatct tttagacaag gggaaaatta cggcaagtga ggatggggtg cttcatctta
     4261 atcctgagac cagtgattct agcatggttg cagaaggtgc cctactagcc caactttatc
     4321 catctttgga aagagaaggg aaagccaaac tcacagccta tctaagttca aaagatgtag
     4381 caagaatcaa ggtcggtgat tctgttcgct atactacgac tcatgatgcc gggaatcaac
     4441 tttttctaga ttctactatt acaagtattg atgcgacagc tactaagact gagaaaggga
     4501 atttctttaa aatcgaggcg gagactaatc taacttcgga gcaggctgaa aaacttaggt
     4561 acggggtgga aggccgcttg cagatgatta cgggcaagaa aagttatcta cgttattatt
     4621 tggatcaatt tttgaacaaa gagtaatgtt cgtgttttta gagttaaata atttttaaac
     4681 tgtgagaaag attcttcttg cagttttttc tttacatttt ttgaaaaaca tctgctattt
     4741 attcggttaa attcttgtgt tttttggttt tttgtggtaa aatgtgctca agtaatacga
     4801 aaggcgaact ttaaaatgtc aaaacaattg atctattcgg gaaaagctaa agatatctat
     4861 acaactgagg atgaaaatct tattatttca acttacaagg accaggcgac tgctttcaac
     4921 ggtgtcaaga aggagcagat tgcaggtaag ggagtcttga ataatcagat ctcatctttt
     4981 atttttgaga aattaaatgc ggctggtgtg gcgactcact ttgtggagaa actttcagac
     5041 acggaacaac tcaataaaaa ggttaagatt attcctttgg aagtcgtgct ccgcaactat
     5101 actgctggtt ccttttcaaa acgttttggt gtggatgagg gaatcgcctt ggagactccg
     5161 attgtcgaat tttactacaa aaatgatgat ttggatgatc catttatcaa tgatgagcat
     5221 gtgaaattcc tacagattgc ggatgaccag cagattgcct acttgaagga agaaacgcgt
     5281 cgtatcaatg aactattgaa agtctggttt gctgagattg ggcttaaatt gattgacttt
     5341 aagctagagt tcggttttga caaggatggc aagattatct tggcagacga attttcacca
     5401 gataactgcc gcttgtggga tgctgatggc aaccacatgg ataaggatgt tttccgtaga
     5461 ggattgggag aactaaccga cgtttatgag attgtttggg aaaagttgca ggaattgaaa
     5521 taatctgttt gcaatggaaa accttcgtct ctcaactaaa aggactcagg ctgaaaaggt
     5581 cccccagacc ttttcactct gtagagaact aggtgaacca accgatgttt acgaaattgt
     5641 ctgggaaaag ttgcagggtt taaaataaca acctcaaggc tgtttgggaa tattgcaaga
     5701 gctgaaataa aggaataaga attgatggat aaacgtattt ttgttgaaaa aaaggctgat
     5761 tttcaggtca agtcagagag tttggttaga gaactccagc acaacttggg actgtcaagc
     5821 ttgaaaagta ttcgtattgt gcaagtatat gatgtatttg acttggttga ggacttgttt
     5881 gcacctgcag agaagcacat tttctctgag caggtaaccg accatgtttt agatgaagta
     5941 tctgtgcagg cggatcttgc taactatgct ttctttgcca ttgaaagtct gccagggcag
     6001 tttgaccagc gtgcagcttc gtcacaggaa gccttgcttt tgttgggaag ttcgagtgac
     6061 gtgacagtca acacagccca actttacttg gtgaataaag atattgatgc gactgagttg
     6121 aaagctgtca aaaactacct gctcaatcca gttgattctc gtttcaagga tatcacgaca
     6181 gggattgcca agcaggagtt ttcagagtca gacaagacca ttccaaaatt gactttcttt
     6241 gaaagctata cagcagaaga ctttgctcgc tacaaggccg aacaagggat ggccatggaa
     6301 gtggatgatt tgctctttat ccaagactac tttaagtcaa tcgggcgcgt gccaactgag
     6361 actgaactta aggttttgga cacttactgg tctgaccact gccgtcatac gacttttgag
     6421 acagagttga aacacatcga cttttcagct tctaaatttc aaaagcaatt gcagtcaacc
     6481 tatgacaagt atattgccat gcgcgaggaa ttaggtcggt ctgaaaaacc acaaaccttg
     6541 atggatatgg cgactatttt cggtcgttat gagcgtgcta atggacgatt ggatgatatg
     6601 gaagtctctg acgaaatcaa tgcctgctca gttgaaattg aagtggacgt tgatggtgtc
     6661 aaggaacctt ggctcctcat gtttaaaaac gaaacccaca accatccaac agaaattgag
     6721 ccatttggtg gagcggctac ctgtattggt ggagctattc gtgatccgtt gtcaggccgt
     6781 tcctatgttt accaagccat gcgtatttca ggtgctggtg atattacagc accgatttcg
     6841 gaaactcgtg ctgggaaatt gccacaacaa gtcatttcta aaacagcagc tcatggttat
     6901 tcttcatatg gtaaccagat tgggcttgcc acaacctacg ttcgtgaata cttccaccca
     6961 ggctttgtag ctaaacgtat ggaacttggt gccgttgttg gtgcggctcc caagggcaat
     7021 gttgtccgtg aaaaacctga agcaggtgat gtgatcatcc ttctcggagg caaaacaggt
     7081 cgtgatggtg tcggtggtgc gacgggctct tctaaggttc aaacagttga gtctgtagag
     7141 actgctggtg ctgaggttca aaaaggaaat gccatcgaag aacgcaagat tcagcgcctc
     7201 ttccgtaatg gcaatgtcac tcgtctgatc aagaagtcca atgactttgg tgcaggcggc
     7261 gtctgtgtgg ctatcggtga attggcagac ggtcttgaaa tcgacctcaa caaggtgcct
     7321 cttaaatacc agggcttgaa tggtacagaa attgccatct ctgaatcaca agaacggatg
     7381 gcggtcgtgg ttcgtcctga agatgtggat gccttcgttg ccgaatgtaa caaagaaaat
     7441 attgatgctg ttgtggtggc gacagtaact gaaaaaccaa atcttgtcat gcactggaat
     7501 ggtgagacaa tcgttgactt ggagcgtcgt ttccttgaca ccaatggtgt gcgcgtggtt
     7561 gtcgatgcca aagttgtgga caaggatgtc aaactcccag aagagcgtca aacatctgct
     7621 gaaacactgg aatcagatac ccttacggtt ctatctgacc tcaaccatgc aagtcaaaaa
     7681 ggattacaga ctgtctttga ctgctctgtt ggacgctcaa cggttaatca cccacttggt
     7741 ggtcgttacc aactcacacc aactgaggca tctgtgcaga aattgccagt tcaacacggt
     7801 gtgactcata ctgcgtcggt cattgctcaa ggtttcaacc catatgtagc tgaatggtct
     7861 ccataccacg gtgctgctta tgcggttatc gaagcaactg ctcgtttggt ggctgctggt
     7921 gccaactggt ccaaggctcg tttctcttac caagagtatt tcgagcgtat ggataaacaa
     7981 gcagagcgtt tcggtcagcc agtagctgct cttctaggtt ctattgaagc acaaattcaa
     8041 cttggcttgc catctatcgg tggtaaggac tccatgtctg gtacctttga agaattgacc
     8101 gttccgccaa ccttggttgc ctttggggtg acgacggcag atagccgtaa ggtgctctct
     8161 ccagaattta aagctgttgg ggaaaatatc tactacatcc caggtcaagc cctctctgca
     8221 gagattgatt ttgacttgat taagaaaaat tttgctcagt ttgaagccat ccaagctgac
     8281 cataaagtga catctgcatc agctgtcaaa tacggtggtg tagttgaaag tttggctctt
     8341 gctacctttg gaaactatat tggtgcagag gtgaccttgc ctgaacttaa aacagctttg
     8401 acagctcaat taggcggctt tgtcttcaca tctcctgaag aaattgctgg agtagagaag
     8461 gttggacaaa cgaaagcaga ctttacactg actgtcaacg gtgtgaagct agatggacac
     8521 aagcttgaca gtgcatttca agggacattg gaagaagttt acccaacaga atttacccaa
     8581 gcgaaagaac tagaagaagt accagctgtg gcatcagatg ttgtgattaa agccaaagaa
     8641 aaggttgaaa aacctgtggt ttacatccca gtctttccag gaaccaactc agaatatgat
     8701 tcagctaagg ccttcgaaaa agaaggtgca gaggtcaatt tggtgccatt cgtgaccttg
     8761 aatgaagaag ctattgtcaa gtcagttgaa actatggttg acaatatcga caagactaat
     8821 attctcttct ttgccggtgg attctcggct gcggatgaac cagatggttc agctaagttt
     8881 atcgtcaata tcctgcttaa tgaaaaagtg cgtgtggcta ttgatagctt tatcgcccgt
     8941 ggtggcttga ttatcggtat ttgtaatgga ttccaagcct tagtcaaatc gggtctccta
     9001 ccctacggaa actttgaaga tgctaacaat actagcccaa ccctcttcta caatgatgcc
     9061 aaccaacacg tggccaagat ggtggaaact cgcattgcca ataccaactc accatggttg
     9121 gctggtgtgc aagtgggcga tatccacgct attcctgttt cgcacggtga agggaagttt
     9181 gtcgtgacgg ctgaggaatt tgcagagctc cgtgacaatg gacaaatttt cagccaatac
     9241 gttgacttta acggtaaacc aagtatggat tctaagtaca atccgaatgg ttctgtccat
     9301 gccatcgaag gaattaccag caagaatggt caaatcatcg gtaagatggg ccattcagaa
     9361 cgttatgagg acggtctttt ccaaaatatc ccaggcaata aagaccagca cctgttcgcg
     9421 tcagcggtta aacatttcac tggaaaataa gacttacaga ttttctaata gatagtatca
     9481 gtaatgtaaa agtcatgtaa atctagctct tgatgattac aaatgaaaat taggtataaa
     9541 aaatgacata cgaagtaaaa tctcttaatg aagaatgtgg tgttttcggt atttggggac
     9601 atccagatgc tgctaagttg acctattttg gactccacag tcttcaacac cgtggtcagg
     9661 agggggcagg aatcctctcc aatgatcaag gacaactgaa gcgccatcgt gacatggggc
     9721 ttttatcaga agttttcaga aatccagcta atttggataa attgacagga gctggtgcga
     9781 ttgggcatgt gcgttatgcg actgctggcg aagcttctgt agataacatc cagcccttct
     9841 tcttccgttt tcacgatatg cagtttggct tggctcataa tgggaatctg accaatgcag
     9901 cctctctcaa gaaagaactg gaacaaagag gagcaatttt cagcgctact tcggactctg
     9961 aaatcttggc tcacctcatt cgtcgtagtc ataatcctag cctgatgggc aaaatcaagg
    10021 aagcgctcag ccttgtcaaa ggtggttttg cctatatctt gctgtttgag gacaagttga
    10081 ttgcggctct tgacccaaat ggattccgac cgctttcgat tggtaaaatg gctaatggag
    10141 cagttgttgt atcttctgaa acctgtgctt ttgaggtcat tggtgccgag tggattcgtg
    10201 atttgaagcc aggtgagatt gtgatcattg atgacgaggg cattcagtat gacagctata
    10261 cagatgatac ccagttggcg gtttgttcta tggagtatat ctactttgct cgccctgatt
    10321 ctaatatcca cggtgtcaat gtccatacgg cacgtaagag aatgggagca caattggcgc
    10381 gagaatttaa gcatgaggca gatattgtag ttggtgtgcc caattcttcc ctaagcgcgg
    10441 ctatgggatt tgcggaagaa tcaggcttac caaatgaaat gggtctgatc aaaaaccaat
    10501 acacccagcg aacttttatc caaccgactc aagaattgcg ggagcaagga gtgcggatga
    10561 aactgtctgc tgtttcgggt gttgtcaaag gcaaacgtgt ggtcatggtg gatgattcca
    10621 ttgtacgtgg aacaacctct cgtcgtatcg ttcagctctt gaaagaagcg ggtgcgactg
    10681 aggttcacgt tgccattgga agtcctgcac tagcgtatcc atgtttctac gggattgata
    10741 tccagacccg tcaggagctg attgcagcca atcatacggt cgaagaaact cgccaaatca
    10801 ttggtgcgga cagtctgact tatctttcaa ttgatggctt gattgagtcg attggtatcg
    10861 aaacagatgc gccgaacggt ggtctctgtg tcgcttactt tgacggtgac tacccaacgc
    10921 ctctctatga ctacgaagaa gactatcgta gaagtttgga agaaaagacc agtttttaca
    10981 agtaggcgac agattctcca ttaaagaaaa ggaaaaaaca aatgacaaat aaaaatgcat
    11041 atgcccagtc gggtgtggat gttgaagcgg gttatgaagt tgttgaacgg attaaaaagc
    11101 acgtggcccg tacggagcgt gcaggtgtca tgggagctct tggtggcttt ggtggtatgt
    11161 ttgacctttc caagactggg gttaaagaac ccgtcttgat ttcagggact gacggtgtcg
    11221 gaaccaagct catgttggct atcaagtacg acaagcacga taccatcggg caggactgtg
    11281 tggccatgtg tgtcaacgac atcattgctg caggtgcgga acccctctat tttctcgact
    11341 acgtagcgac agggaagaat gaaccagcta agctagaaca agtggttgct ggtgtggcag
    11401 aaggttgtgt gcaggctggt gctgccctca tcggtgggga aacggctgaa atgccgggca
    11461 tgtacggcga agacgactat gacttggctg gttttgcggt cggtgtggct gaaaaatctc
    11521 aaatcattga cggttcaaag gtggtagagg gagatgttct tctcggactt gcttcaagtg
    11581 ggattcactc aaatggttac tctttggttc gtcgtgtctt tgcggattac acaggtgagg
    11641 aagtcctacc agaattggaa ggcaagaaac ttaaggaagt tctacttgag ccgactcgta
    11701 tctatatcaa ggctgtcttg ccgctcatca aagaagagtt ggtcaacggc attgcccaca
    11761 tcacaggtgg tggctttatc gaaaatgtcc ctcgtatgtt tgcagatgac ctagctgctg
    11821 aaattgatga aagcaaagtt ccagtgcttc caattttcaa agcccttgaa aaatatggtc
    11881 agattaaaca cgaagaaatg tttgaaatct tcaatatggg tgtgggactt atgttggcgg
    11941 tcagccctga aaatgtagag cgtgtaaaag aattgttgga tgaagcagtc tatgaaattg
    12001 gtcgcatcgt caagaaagaa aacgaaagtg tcattatcaa atgaaaaaaa tagcggtttt
    12061 tgcctctggt aatggctcaa attttcaggt gattgccgaa gaatttccag tggagtttgt
    12121 cttttcagac catcgtgatg cctatgtgct tgagcgtgca aagcagctcg gcgttctgtc
    12181 ctatgctttt gaactcaagg agtttgagag caaggcagac tacgaagcag cccttgtcga
    12241 actcttggaa gaacaccaga ttgacttggt ttgcctagca ggctacatga aaatcgttgg
    12301 accaacctta ttgtcggctt atgaaggtcg gattgtcaac attcatccag cctacttgcc
    12361 agaatttcca ggagctcatg ggattgagga tgcttggaat gctggcgtgg gtcagtctgg
    12421 tgtgaccatt cactgggtgg attcgggtgt ggatacaggc caggtcatca aacaggttcg
    12481 tgtgccacga ctagctgatg ataccattga cagatttgaa gctcgcatcc atgaagcaga
    12541 gtacaggctg tatccggaag tagtgaaggc tctatttaca gattgacttt ttgatgattc
    12601 atatgatatc tttgatttta aattggagtc agtgtttgtt gaagacggct tcaaacggag
    12661 gtatttgtaa tgttagaatc taaaaaaaca actcgatatg tattttatgt ctatctgatg
    12721 ttattaactt ggggaatctt atttaagttt gaaacaaatc ctgaatttat agcatttttc
    12781 ttagctccaa ggtatatcaa ttggattcca ttttcagaac cactaatagt cgatggaaaa
    12841 attgtttttg ctgaaatgtt atttaatctg atttccttta ttccattagg tgtttgtttc
    12901 cctttgataa aaactaattt atctagttta agaatagtcg ggacaggttt cttgattagt
    12961 ttattgtttg agtgcttaca gtatatttta gcaataggta taacagatat aacggatttg
    13021 actttaaata cgctaggtgt ctgtgtaggc ttactgattt atcaaatttt tataagagtg
    13081 ttcaaatcac agactagaaa atggatcaat atcttaggta tgcttagcct tggttttgct
    13141 tatcttgttt tactgttact gcatttaatt ggtgtttaac taatgattaa aaaggagaat
    13201 ataatgacta aacgcgtctt aatcagcgtc tcagacaaag cgggcattgt tgaatttgcc
    13261 caagaactca aaaaacttgg ttgggagatt atctcaacag gtggaactaa ggttgccctt
    13321 gataatgctg gggtggatac cattgctatc gatgatgtga ctggtttccc agaaatgatg
    13381 gacggtcgtg tgaagaccct ccacccaaat atccacggag ggcttctcgc tcgtcgtgac
    13441 ttggatagcc acttggaagc ggctaaggac aacaagattg agctcattga ccttgtggtg
    13501 gtcaaccttt acccatttaa ggaaactatc cttaaaccag atgtgactta tgctgatgca
    13561 gttgaaaata tcgatattgg tgggccatct atgcttcgtt cagcagcgaa aaatcatgcc
    13621 agtgttacag ttgtggtaga tcctgctgac tacgctgtgg ttttggacga attggcagca
    13681 aacggcgaaa cctcttatga aactcgccaa cgtttagcag ccaaagtatt tcgtcacaca
    13741 gcggcttatg acgccttgat tgcagaatac ttcacagctc aagtgggtga aagcaagcct
    13801 gaaaaactca ctttgactta tgacctcaag caaccaatgc gttacggtga gaatcctcaa
    13861 caagacgcgg acttttacca gaaagctttg cctacagatt actctattgc ttcagccaaa
    13921 cagctcaacg ggaaagaatt gtcatttaat aatatccgtg atgcagatgc tgctatccgt
    13981 atcatccgtg acttcaaaga tagtccaacc gttgtggctc tcaaacacat gaatccatgt
    14041 ggaattggtc aagctgatga catcgagact gcttgggact acgcttatga gtctgaccca
    14101 gtatctatct ttggtgggat tgtcgtcctc aaccgtgagg tggatgctgc gacagctgag
    14161 aagatgcacg gcgttttcct cgaaatcatc attgcaccaa gctatacgga tgaagcgcta
    14221 gctattttga tcaataaaaa gaaaaacttg cgtatccttg ccttgccatt taatgctcaa
    14281 gaggctagcg aagtggaagc agaatacaca ggtgtagtcg gtggacttct cgtgcaaaat
    14341 caagacgtgg tcaaggcaag cccagctgac tggcaagtgg tgaccaaacg tcagccaact
    14401 gagacagaag cgactgctct tgagttcgct tggaaggcta tcaagtacgt caaatcaaat
    14461 ggtattatcg tgaccaacga ccacatggca cttggtgttg gtccaggtca aaccaaccgt
    14521 gtggcttctg ttcgccttgc cattgaccaa gccaaagatc gtctgaacgg ggcggtcctt
    14581 gcttcagatg ccttcttccc atttgcggat aacgtggaag aaatcgccaa agcaggaatt
    14641 aaggccatca tccagcccgg tggctctgtc cgtgaccaag aatccatcga agcagcggat
    14701 aaatacggct tgactatggt ctttacaggt gtgagacatt ttagacatta agaagataaa
    14761 agggaagaaa acagtttctt tccttttttg gcttaaaatg ctaactgaaa caagattaaa
    14821 acgaactttt ttgatataat attggtaaat aattcgcaaa agaggttgag gaatgaaact
    14881 gcttgttgtc ggttcgggtg gtcgtgagca tgcgattgct aaaaagttac ttgaatcaaa
    14941 agacgttgaa aaagtctttg tagctcctgg gaatgatggg atgactctgg atggtttgga
    15001 attggtaaat atctctattt ccgaacattc taaattgatt gacttcgcaa agaccaatga
    15061 tgttgcttgg acctttatcg gtccagatga tgcccttgct gctggtatcg tggatgattt
    15121 taaccaagct gggcttaagg cctttggtcc gactagggct gcagcggagc tggagtggtc
    15181 caaggatttt gccaaggaaa tcatggtcaa atatggtgtt tcgacagcag cctatggcac
    15241 attttcagat ttcgaggaag caaaggccta tatcgaaaag catggtgcgc ctatcgtagt
    15301 caaggcggat ggcttggcgc ttgggaaggg tgtcgtcgtt tctgagacgg ttgagcaagc
    15361 ggtcgaagcc gctcatgaga tgcttttgga caataaattt ggtgactcag gtgcgcgcgt
    15421 ggttattgag gaattccttg aaggagagga attttcactc tttgcctttg ttaatggtga
    15481 taagttctac atcatgccaa cggctcagga ccataaacgt gcctacgatg gcgacaaagg
    15541 gcctaacacg ggtggtatgg gtgcctatgc gccagttcca cacttaccac agagtgtagt
    15601 tgatacagcg gttgacacca ttgtcaagcc agttctagaa gggatgatta aagaaggtcg
    15661 cccttatctg ggagttcttt acgcagggct tatcctgaca gctgatggac cgaaagtcat
    15721 tgagttcaac gctcggttcg gagatccaga aactcagatt atcttgcctc gcttgacctc
    15781 tgactttgct caaaatatca cagatatcct ggatagcaag gagccaaata tcatgtggac
    15841 ggacaagggt gtgactctgg gtgtggttgt cgcatccaag ggctacccgc tagactatga
    15901 aaggggcgtt gagttgccag ccaagacaga aggcgatgtc atcacctact atgcaggggc
    15961 taagtttgcg gaaaatagca gagcactgct ctcaaacggc ggacgagttt atatgctcgt
    16021 taccacagca gataccgtca aagaagccca agccagcatc taccaagaac tataccaaca
    16081 aaaaatagaa ggactcttct accgaacaga tatcggaagc aaggcaatta agtaaagata
    16141 taagaataac gcgccgtagt cgccaaacac gataatggtc gtcgtggtga aaagaccaga
    16201 acagtgaatg ttctggtcag ggggaaactt ggagaccgta ggctcaaagt ttaggaatga
    16261 aaccgaaggt ttgcttccgc ctccatcacc taagaccatt atcaaaaaga aaaataaaaa
    16321 ttcacaaaat acgttaatga tcgtatggtt tgcgagcgtt agcgagctaa tatagaacaa
    16381 tcaccgccgt tgtgaaagaa cgattggatg ataatccaat cgttcaggga aattggaaga
    16441 ccttgggttt ccaatttagg catgagacac ctttggtggc tgctgccgtc cctcacaagc
    16501 taaggtgatt gttgaaaaag aggaaaaagg agaagaaatg aaaccagtaa tttccatcat
    16561 catgggctca aaatccgact gggcaaccat gcaaaaaaca gcagaagtcc tagaccgctt
    16621 cggtgtagcc tacgaaaaga aagttgtttc cgcacaccgt acaccagacc tcatgttcaa
    16681 acatgcagaa gaagcccgta gtcgtggcat caagatcatc atcgcaggtg ctggtggcgc
    16741 agcgcatttg ccaggcatgg tagctgccaa aacaaccctt ccagtcattg gtgtgccagt
    16801 caagtctcgt gctcttagtg gagtggattc actctattct atcgttcaga tgccgggtgg
    16861 ggtgcctgtt gcgaccatgg ctatcggtga agctggagcg actaacgcag ctctctttgc
    16921 cctccgtctc ctctctgtag aagataagtc cattgcggat gcacttgcca actttgctga
    16981 agaacaagga aaaatcgcag aggagtcgtc aaatgagctc atctaaaaca atcggaatta
    17041 tcggtggcgg tcaactgggt cagatgatgg ccatttctgc tatctacatg ggccacaagg
    17101 ttatcgcgct ggatcctgtg gcggattgcc cggcctctcg cgtggcggaa atcattgtgg
    17161 caccttataa cgatgtggac gccctccgtc agttggcaga ccgttgcgat gtcctcactt
    17221 atgagtttga aaatgtcgac gctgacggtt tggatgccgt tatcaaggat ggacaactcc
    17281 ctcaaggaac agatctgctc cgcatttcgc aaaatcgtat ttttgaaaag gactttttgt
    17341 caaacaaggc tcaagtcact gtggcaccct acaaggtcgt gacttctagc ctagacttgg
    17401 cagatatcga cttgtcgaaa aactatgtcc tcaagactgc gactggtggc tacgatggtc
    17461 atggacaaaa ggttattcgt tcagaagcag acttggaagc agcctatgcg ctagcagact
    17521 cagcagactg cgtcttggaa gaatttgtca actttgacct tgagatttct gtcatcgtgt
    17581 caggaaatgg caaggaggtg acgtttttcc cagttcagga aaatatccac cgcaacaata
    17641 tcctgtctaa gaccatcgta ccagcccgca tttctgaaag tctagtagac aaggctaaag
    17701 ctatggcagt gcgaatcgca gaacaactca acttgtctgg aactctctgt gtggaaatgt
    17761 ttgcgactga tgatgacgtt attgtcaatg aaatcgcccc acgaccacat aactctgggc
    17821 actattctat tgaagcctgt gatttctctc agtttgacac ccatattctc ggtgttctcg
    17881 gagcaccatt accagtcatc aaactccatg cgccagccgt tatgcttaat gtcctcggtc
    17941 agcatgtcga ggctgctgag caatatgtga cagaaaattc aagcgcccac ctccacatgt
    18001 atggtaaaat agaagcgaag cacaaccgca agatgggaca tgtgaccttg tttagtgatg
    18061 taccggatag tgtggatgag cttggggaag ggattgattt ttaggacaag tctatgatac
    18121 aaattatcgt taatacattt attgaaaagg ataagactgg agcagttgtt gaagtgttgt
    18181 atgccagtgc tgaccaagat aaggtacaag ctaaatatga agaactagct gcacaatacc
    18241 ccgaaaatta tttagctatc tatgatgtac cgctggatac ggatttgaat acactagatc
    18301 attacccgtc tgtgtttatt gaaaaagagg agtttgagta gaaatcttgg tttacctaga
    18361 tagcttattc ccaacagctt aagaagaaag gaaaaattaa cacatgatca accgttactc
    18421 tcgccctgag atggcgaata tttggagtga agaaaataaa taccgtgctt ggcttgaggt
    18481 ggaaatcctc tctgacgagg catgggctga gttgggggaa atccctaagg aagatgtggc
    18541 tttgattcgc aagaaggcgg actttgacat cgaccgtatt ttggaaattg agcaggagac
    18601 gcgccacgat gtggtggctt tcacgcgtgc ggtttctgag actcttggtg aagagcgcaa
    18661 gtgggttcac tatgggttaa cttctaccga cgtggtggat actgcttatg gttacctcta
    18721 caagcaggcc aacgacatca tccgtcgtga ccttgaaaac ttcactaata tcatcgctga
    18781 caaggccaag gagcacaagt tcaccatcat gatggggcgt actcatggtg tgcacgctga
    18841 gccgacaacc tttggtctta aattggcaac ttggtacagc gaaatgaaac gcaatatcga
    18901 gcgtttcgag catgcagctg ctggtgtaga agctggtaag atttctggtg cggttgggaa
    18961 ctttgccaat atcccaccat ttgtagagga gtatgtctgc gataaacttg gcatccgtgc
    19021 ccaagaaatc tctacacaag tccttcctcg tgaccttcac gctgagtact ttgcggttct
    19081 tgccagcatt gcgacttcaa tcgaacgtat ggcgactgag attcgtggtc tacaaaaatc
    19141 tgagcaacgc gaagtagaag agttctttgc taaagggcaa aaagggtctt cagcaatgcc
    19201 tcacaaacgc aacccaatcg gttctgaaaa tatgactggt ctggcgcgtg tcattcgtgg
    19261 tcacatgatt acggcttatg aaaacgtcgc tctctggcac gaacgcgata tttctcactc
    19321 atcagctgag cgtatcatca caccagatac gaccattttg attgactaca tgctcaaccg
    19381 ttttggaaat atcgtcaaga acttgacagt cttcccagaa aatatgatcc gaaacatgaa
    19441 ctcgactttt ggtcttatct ttagccaacg ggctatgttg acattgattg aaaaaggcat
    19501 gacccgtgag caagcctatg acttggtgca accaaaaaca gcctactctt gggacaacca
    19561 agtagacttt aaaccacttc ttgaggcaga ttcagaagta acatcacgtc tcactcaaga
    19621 agaaatcgat gaaatcttca acccagttta ttacaccaaa cgagtggatg atatctttga
    19681 acgtcttgga ctaggtgatt aattaaaaaa taaacagcga gcttcaatct cgctgtttat
    19741 tttttatcga aaagacttag tcttcttttc ttttagtgag tccataggct gctagtgtgg
    19801 acatgagtcc tgcgactact agtcctgcag aatcgtgagt tcctgtttca ggaagttttt
    19861 tctctgttac cacaggagct ggatcttgag gaagagcttt gctttcctca gcgggagcag
    19921 ttgatggagc tggttggctt gggatttcta gttttggttt ttcttcagca atagcggctt
    19981 gtccgttttc atcgcctaca tgtgttacca tagttccgac ttcgactatt tgagtaacgg
    20041 cttcctgtgc tacgacacta tttacaagtg ttttcacttc cttaccatcg gcagaagtgc
    20101 tcacagagta gaagttgcta cgatgtccat tgacgccctt agtaatgact tgtgtttttc
    20161 ctttgagtaa gagtggattt tcacgagtca ctgtggtaaa tggaatttct tctttttgga
    20221 tatccagtct aggttttacc tcagtagttg gtgcaagacc actttcatca cccttgtgag
    20281 ttacaggagc gccaacttca accacttggt ttataacttc tttggttacc tggctatcaa
    20341 ggactgtttc tgttgttttt ccattttcag tgagtacaga gatgtaatga gttcgttcac
    20401 ctttgactcc tgctgtgata atattttcct gaccagctgg gaggttagga ttttctttct
    20461 tgataacttc aaatggaatt tcttcagttc ttgtgatgag ttctggtctg gtttcaacat
    20521 tggcagccac ttcattttca tccaggcttc ctgaatgagt tgcagctggt ttgaggcctt
    20581 gaagagcggc ttttaggttg gctacaagtg tgtcaagctc agcttgttta ttacggttga
    20641 ggttgtaatt tagagctgtt ttagctgcgt caagggcctc aagactttct ttactatatc
    20701 cttctaagtt tgtaggaatt ttagctaatt cttcgcggag agcattataa ttagcacgga
    20761 agtagtcttt gttgtggtct gcaaaggcag tcatgagttc aaagatttcc tcttccttgt
    20821 actcagcgct tggtctatct gcccagattg aaagcatact tccgactgtt ggaagatcta
    20881 cttcaggata tttggtagaa gctagttgat tgaatggtgt ttttccagta ttctcaatag
    20941 ctttcttgag gaaaccacca ccatcttctg gtttttgacc aagaatgtag taccagtcac
    21001 cgttggtatt caagaattta tagcctttgc ttgctaggta ttgaggtgat gcgaggttat
    21061 atccccacca gcctttagac cagtaagaaa tcaagacatc tttgtcaaac tgaacatcgt
    21121 ccttgtcttc atagtagaag ccatcgttga aggccattgg ttgaagccct ctttctttgg
    21181 ccatagctgc gagggtgttg gcatattcgg caaatttgcc atagagttga taccacttga
    21241 ggtagtacca gccttgggca ctagtcgcat cgttggcgta ttcgtcagta ccaaagttga
    21301 aaatctttgt tttacctgca aagaagtcca tgtatttacc gatgagggct tttacaaagt
    21361 tcatcgcttc ttcgtttttc aagtccatag ttgtttttga aactttatca aagtgggctt
    21421 gaggattttt aatacctaat ttttccatgg caaccagcat agcatccatg tgacctggac
    21481 tgttaatagc tgggatgaga ccgatgtcct tagatttagc gtattcaatt agctctgtta
    21541 cttctgcctg tgttagtgca gtaccgtttg gatcgtcgta gtaagcttta gttccttcga
    21601 taatagcttt tttaacgtca tcactagcat aggtttttcc gttggcagta atggtcatat
    21661 catcgagtag aaagcgaagt ccgtcatttc ctagaaggag atggacatca gaatatccga
    21721 gctcactggc cttgtctacg atgcgtttga gctggttcag agtaaagtat ttgcgtccag
    21781 catcgattga gattaccttg tttttggcaa gtttttcaac ctcacgttta gcttcttctt
    21841 cttttagagc ttcaggcgtg agggtcaagt tgttgacagt ttcttgaagt ttagcaatgg
    21901 cttgatcaat cgtatcttgt tgggcacggc taaggttgct atcgagagag cgaatagctt
    21961 tttcagcttc ttttacggcc gtgacgcttt ctgcagtata acggttcagg tcttttggta
    22021 cctcgttaag tgcttgctct gcagattcat aatcagctgc gaagtattca gcgttggcat
    22081 ttgcaaaatg acgcatgagt ttgaagaggc gtgatggtga ataacgtgca gatggagtgt
    22141 cagcccaagc agctaccata ccaccgatga ttgggatatc agctccttct gtttttggta
    22201 cagaagtgat tggtgtgttt ttaataccat tgagcccctg atcgagattg taccagcctt
    22261 ggccatcagc gtttcgtcca agaacgtagt accaagcatc attggtatta aggatttggt
    22321 gacctttttc agctagtagt ttagaagaag cgacatcgta gcctccccaa ccaccagtcc
    22381 acatagaaac gatgatgtct ttgtcaaaac taccaaagct tgtgtcgcta ttgtagtaga
    22441 taccgtcgtt aaaagccatt ggtttgagac cgtgcgattt tacaatacga gcgaggtcat
    22501 tggcgtaggc aataaatttt tcatagcctt ttacagggta gccttcgttt ggatagtatt
    22561 tatcagcttg aagcacactc caacctttag catctgtcgc atcattggca tattcatcaa
    22621 gtccgatgtt gaagatttca gtctttttcg cgaaataagc agcatacttg tcgataaggg
    22681 cttttgtaaa agcgacagct tgttcgttgt caagatcgac agtacgggct gatttcttcc
    22741 caaaatagct aaagttaggg ttttggattc ccaattcttt catggcattg agaatcgcat
    22801 ccatgtgtcc aggactattt actgtcggaa tgagaccgat acctttatct ttggcatagt
    22861 taatcagatc tgtcatttga ctttctgtta agtgattgcc gtttggatcg ttgtaataat
    22921 catttgtacc tttttcaatg gcgcgtttga catcgtcact ggcataggtc ttgccgttag
    22981 ctgtgatgct catatcgtcc aacatgaaac ggagtccatc atttccgact aataggtgta
    23041 aatcagtgta gccataatgt ttcgctttat cgatgatttc cttgagttgt tctggtgaga
    23101 aatatttacg tccagcatca atagaaacaa ttttcttttt cgctagtttt tcatttacag
    23161 ttgcagcacg ttcctttcct gcctctgttt ccggtttgtc agcctctgct ttcgcttcat
    23221 cttttttagc tggtttatcc ttgtcagtct tgtctgtatt tgactcttta gaatcaacct
    23281 ctttcgcttc ttccttttta gggctagctt cttctgcctt tttattagca gtttcttttt
    23341 cagcagaagt tggagttacc acttctgctt tatcactagg agttgaacta acttcctctt
    23401 gtggtttttc ttctgttttt ggaagactag ctaccttatc agtagctgga gtttctgttt
    23461 ctacagtttt tggagcttct ggttgaagca ctgctttagg tgtttcctca gtccgatttt
    23521 cggatgattg aggggaatca gaaaccgtat ggatggtcgg ttggttttct gtagtagtag
    23581 gagtaactcc atcggctgca acagtctgtg cttggaaggc aaatccaatt agaacagaag
    23641 ctgctcctac agcgtattta cgaatagaaa aacgctgttg tttttcatgt ttcattgcaa
    23701 aacctcctga ttgcattgtt atattgatag cgattatata aatcaacgcc tttattttat
    23761 ttcttgtttt aacgagagtc aagaggagat gacaaaaaac tataataagt ataaaaaaat
    23821 ataaaattta aacttaagat ttcagattgg tcggaaaaaa tacgtatata tatctagtat
    23881 aatttttggt tctatttcta taaaatattc cacaaattat agaattttcc aaaaataggt
    23941 aagcgctacc tttttggtgt ggtataataa gcatagaaaa agcccaagcg attagctcag
    24001 gctttcttct tagtgttcac ggtcacatga gataaattta atcttgtagt aatcagatcg
    24061 tttgtaagtt tcactgtatt ctaaaacttg gccagttgat tcgagtttgg tgattttagt
    24121 ttgtaggaca gtagggaatt gttcatcgac tccgaggact gaagccgcat gttctggagt
    24181 tggaaagact atttcgttga tttcttcaaa gtgttcatca ttcatgtgaa tgtggtagtc
    24241 taacttgaaa cgattataga tagaactata gtattcaagg tttggataat ttgcgttgat
    24301 atattgttct gggatgtagg atgtatggta gatataaacg acaccgtttg attcgcggat
    24361 acgttcaatc ttgtagtaga attgatcgcc acgtagaccc aatttttcca agtaaacaag
    24421 cttgtttccg cgttcaattg aaagaacagt taccttatca tctttagcat tgaagagttc
    24481 aatatctgaa aactctacaa gcttgtgttt gcgtgcacgt gaaacgaaag ttccttttcc
    24541 ttgttggcgg acaatatagc catctttggc aaggtcgttt aaggcgcgaa caactgtgat
    24601 agagctgaca tcgtacattg aaatgagttc tgcttcagtg taaaatttat ctccactgct
    24661 aaactgccca gagatgattt tattttttaa ttcgtctttt atgtattgat atttgggaat
    24721 tgccatattt ttacctctat tttccttctc catagaaaat tttaccataa aagcgaaaaa
    24781 aatagtagtc ttttgaataa aaaattagaa taatagtttg aaaaat
//
LOCUS       DAVKJU010000027        23313 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVKJU010000027 DAVKJU010000000
VERSION     DAVKJU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23313
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.027"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            323..1426
                     /gene="glf"
                     /locus_tag="VW191_001050"
     CDS             323..1426
                     /gene="glf"
                     /locus_tag="VW191_001050"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU9623833.1"
                     /translation="MYDYLVVGAGLFGAVFAHEAALKGKKVKVIEKRNHIAGNIYTRE
                     EEGIQVHQYGAHIFHTSDKEIWDYVNQFAEFNRYTNSPVANYKGEIYNLPFNMNTFNK
                     LWGVVTPAEAQAKIEEQRAILNGKTPENLEEQAISLVGTDIYEKLIKDYTEKQWGKPT
                     TELPSFIIRRLPVRLTYDNNYFNDTYQGIPIGGYTQIVEKMLDHENIDVETNVDFFVN
                     KEQYLKDFPKIVFTGMIDEFFDYKLGELEYRSLRFENETLDMENYQGNAVVNYTDAET
                     PYTRIIEHKHFEFGSQAKTIITKEHSKTWEKGDEPYYPVNNDRNNHLYKSYKKLADEQ
                     GNVIFGGRLGHYRYYDMHQVIGVALQCVRNELN"
     gene            1747..3729
                     /locus_tag="VW191_001051"
     CDS             1747..3729
                     /locus_tag="VW191_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623834.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQIGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
     gene            4030..9333
                     /locus_tag="VW191_001052"
     CDS             4030..9333
                     /locus_tag="VW191_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9623835.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKTAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTTLVAED
                     FASLDAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TNNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(9500..11659)
                     /locus_tag="VW191_001053"
     CDS             complement(9500..11659)
                     /locus_tag="VW191_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU9623836.1"
                     /translation="MNKQTILRIAKYVSICFLTLFITAVMLGGGIFLYYVSKAPALSE
                     SKLVATTSSKIFDSKNELIADLGSERRVNAQANEIPTDLVKAIVSIEDHRFFDHRGVD
                     TIRIIGAFLRNLQNNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAVQLEQK
                     TTKQEILTYYVNKVYMSNGNYGMQTAAQNYYGKDLRELSLPQLALLAGMPQAPNQYDP
                     YSHPEAALDRRNLVLSEMKGQGYISAEQYEKAINTPITDGLQSLKSVNSYPVYMDNYL
                     KEVIDQVEQETGYNLLTTGMDVYTNVDQEAQKRLWDIYNSDQYVSYPDDDLQVASTVV
                     DVSNGKVIAQLGARHQASNVSFGTNQAVETNRDWGSSMKPITDYAPALEYGVYDSTAT
                     MVNDIPYNYPGTSTPVYNWDRAYFGNISLQYALQQSRNVPAVETLNKVGLDRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESSKQYGASSEKMAAAYAAFANGGIYHKPMYINKVVFS
                     DGSEKEFSDAGTRAMKETTAYMMTEMMKTVLTYGTGRGAYLPWLPQAGKTGTSNYTDD
                     EIEKYIKNTGYVAPDEMFVGYTRKYSMAVWTGYSNRLTPIIGDGFLVAAKVYRSMISY
                     LSEDDHPGDWTMPEGVYRSGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     LTTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(11656..12252)
                     /gene="recU"
                     /locus_tag="VW191_001054"
     CDS             complement(11656..12252)
                     /gene="recU"
                     /locus_tag="VW191_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEU9623837.1"
                     /translation="MVNYPHKLSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVRVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEAKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLGYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            12319..12846
                     /locus_tag="VW191_001055"
     CDS             12319..12846
                     /locus_tag="VW191_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEU9623838.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGTLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            12916..13257
                     /gene="gpsB"
                     /locus_tag="VW191_001056"
     CDS             12916..13257
                     /gene="gpsB"
                     /locus_tag="VW191_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEU9623839.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYSKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELARKPQVSSAPSPSHPDPIDVAASSSMTNFDILKRLNRLEKEV
                     FGKQILDNPDL"
     gene            13278..13658
                     /gene="rnpB"
                     /locus_tag="VW191_001057"
     ncRNA           13278..13658
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VW191_001057"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13743..14900
                     /locus_tag="VW191_001058"
     CDS             13743..14900
                     /locus_tag="VW191_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU9623840.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     TIIETNLWLRAADRIKIIVGTFQAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMATAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            14913..16307
                     /gene="mapZ"
                     /locus_tag="VW191_001059"
     CDS             14913..16307
                     /gene="mapZ"
                     /locus_tag="VW191_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEU9623841.1"
                     /translation="MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSHLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSLSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            16383..17807
                     /gene="gndA"
                     /locus_tag="VW191_001060"
     CDS             16383..17807
                     /gene="gndA"
                     /locus_tag="VW191_001060"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEU9623842.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            17819..18508
                     /locus_tag="VW191_001061"
     CDS             17819..18508
                     /locus_tag="VW191_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623843.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLMLLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            18607..19629
                     /gene="cbpC"
                     /locus_tag="VW191_001062"
     CDS             18607..19629
                     /gene="cbpC"
                     /locus_tag="VW191_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEU9623844.1"
                     /translation="MKLLKKMMQVALAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            19648..20646
                     /gene="cbpJ"
                     /locus_tag="VW191_001063"
     CDS             19648..20646
                     /gene="cbpJ"
                     /locus_tag="VW191_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEU9623845.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLKGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKYSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGLMATGWYQEGTTWYYLDQSNGDMKTGWQYLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(20764..21996)
                     /locus_tag="VW191_001064"
     CDS             complement(20764..21996)
                     /locus_tag="VW191_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9623846.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(22039..23001)
                     /locus_tag="VW191_001065"
     CDS             complement(22039..23001)
                     /locus_tag="VW191_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEU9623847.1"
                     /translation="MTSSTEFGNFVSNLSFQHGIAGLLFPLNKLYPPELDSKILSIIK
                     KAVTIRTTHTYEYQYSLLFGDAGYLWLLLHLFSISKNQYYLQLANVTAKKLIENYDTL
                     EEIDFALGKSGVLLSLIKYYQFTNDNTLKIFIHNSIGEIYHYFLQRDTAKESILDYSF
                     AHGYCGIAYALFAYSKVLEPSMFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKG
                     ISGIILYLCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLM
                     KKIQQVILACSERDDHGLLMFQGDSGKADLFDFGIGSMGYIGVY"
BASE COUNT         7367 a         4097 c         5156 g         6693 t
ORIGIN      
        1 ttaccataat ttttttgctg aatattttta tgatatttta ttagtaaaag ttgtaagcgt
       61 agggattttt ctaactattt tctcaattgt attgaatgaa aatagcaaca aatggattat
      121 ttttctttct aatcaaacta tgggtatctt tataatacac acttatatta tgaaggtatg
      181 ggaaaaacta tttggtttta attttatagg ttcatattta ctttttgcta tatttacttt
      241 aagtgttagt tttatcattg ttggaatgtt aatgaaaatt ccgtatttta atcgaatcgt
      301 caaattataa aaaggagaaa aaatgtacga ttatcttgtt gttggtgctg gtctctttgg
      361 tgcagtcttt gcccatgaag cagccttaaa aggaaaaaaa gtaaaagtta ttgaaaaacg
      421 aaatcatatc gcgggtaata tctatactcg tgaagaggaa ggaattcaag ttcatcaata
      481 tggtgctcat atcttccata cttctgataa ggagatctgg gattatgtaa atcagtttgc
      541 agagtttaac cgttatacca attctcctgt tgcaaactat aagggtgaga tttataacct
      601 tccttttaat atgaatactt tcaataaact ttggggagtt gtaacgccag cagaagcaca
      661 agctaagatt gaggaacaac gtgctatttt aaatggtaaa actcctgaaa atttggaaga
      721 acaggcgatt tctcttgtag gtacagacat ctacgaaaaa ttaatcaaag actatacaga
      781 gaaacagtgg ggcaaaccaa ctactgaact tccatccttt attattcgcc gtttgccagt
      841 acgcctgacc tatgataaca actattttaa cgatacctat caagggattc caattggtgg
      901 atacactcaa atagttgaaa aaatgttgga tcatgaaaat attgatgtag aaacaaatgt
      961 tgatttcttt gtgaataaag agcaatatct gaaagatttt cctaagattg tctttactgg
     1021 tatgattgat gaattctttg actataagtt gggcgaacta gagtaccgta gtcttcgttt
     1081 tgaaaatgag accttggata tggaaaatta ccaaggaaat gcagttgtga actatacgga
     1141 tgcagaaact ccatatactc gcattattga acacaaacat tttgagtttg ggagtcaagc
     1201 aaagactatc attactaaag aacattctaa aacatgggaa aaaggtgatg agccttatta
     1261 tccagttaat aatgatcgta ataatcattt gtataaatca tacaaaaaac ttgctgatga
     1321 gcaagggaat gttatctttg gtggccgctt aggacactat cgttattacg atatgcacca
     1381 agtaattgga gtagctttgc agtgcgtgag aaatgagtta aattaatact caatgaaaat
     1441 taaagagcaa actaggaagc tagccacagg ttgctcaaaa cactgttttg aggttgcaga
     1501 tggacgctga cgcggtttga agagattttc gaagagtata aacaagtaaa actgactacc
     1561 agttattatt tagaaatagt attaaaaatt ccttgactat gtgatatagt tgagggattt
     1621 ttaaatgata ttcatatttt ttgcaaagat gttgtttgaa aaataatttt caaaaattct
     1681 gaaaattctg ttgacaactt tctgaaaaga gtctataatg gagagaaagt tttaaaggag
     1741 aaaatgatga aaagttcaaa actatttgcc cttgcgggcg tgacattatt ggcggcgact
     1801 actttagctg catgctctgg atcaggttca agcactaaag gtgagaaaac attctcatac
     1861 atttatgaga cagaccctga taacctcaac tatttgacaa ctgctaaggc tgcgacagca
     1921 aatattacca gtaacgtggt tgatggtttg ctagaaaatg atcgctacgg gaactttgtg
     1981 ccgtctatgg ctgaggattg gtctgtatcc aaggatggat tgacttacac ttatactatc
     2041 cgtaaggatg caaaatggta tacttctgaa ggtgaagaat acgcggcagt caaagctcaa
     2101 gactttgtaa caggactaaa atatgctgct gataaaaaat cagatgctct ttaccttgtt
     2161 caagaatcaa tcaaagggtt ggatgcctat gtaaaagggg aaatcaaaga tttctcacaa
     2221 gtaggaatta aggctctgga tgatcagaca gttcagtaca ctttgaacaa accagaaagt
     2281 ttttggaact caaaaacaac catgggtgtg cttgcgccag ttaatgaaga gtttttgaac
     2341 tcaaaagggg atgattttgc caaagcgacg gacccaagta gtctcttgta taatggacct
     2401 tatttgttga aatccattgt gacaaaatcc tctgttgaat ttgcgaaaaa tccgaactac
     2461 tgggataagg acaatgtgca tgttgacaaa gttaaattgt cattctggga tggtcaagat
     2521 accagcaaac ctgcagaaaa ctttaaagat ggtagcctta cagcagctcg tctctatcca
     2581 acaagtgcaa gtttcgcaga gcttgagaag agtatgaagg acaatattgt ctatactcaa
     2641 caagactcta ttacgtatct agttggtaca aatattgacc gtcagtccta taaatacaca
     2701 tctaagacca gcgacgaaca aaaggcatcg actaaaaagg ctctcttaaa caaggatttc
     2761 cgtcaggcta ttgcctttgg ttttgatcgt acagcctatg cctctcagtt gaatggacaa
     2821 attggagcaa gtaaaatctt gcgtaatctc tttgtgccac caacatttgt tcaagcagat
     2881 ggtaaaaact ttggcgatat ggtcaaagag aaattggtca cttatgggga tgaatggaag
     2941 gatgttaatc ttgcagattc tcaggatggt ctttacaatc cagaaaaagc caaggctgaa
     3001 tttgctaaag ctaaatcagc cttacaagca gaaggtgtga ctttcccaat tcatttggat
     3061 atgccagttg accagacagc aactacaaaa gttcagcgcg tccaatctat gaaacaatcc
     3121 ttggaagcaa ctttaggagc tgataatgtc attattgata tccaacaact acaaaaagac
     3181 gaagtaaaca atattacata ttttgctgaa aatgctgctg gcgaagactg ggatttatca
     3241 gataatgtcg gttggggtcc tgattttgcc gatccatcaa cttaccttga tatcatcaaa
     3301 ccatctgtag gagaaagtac taaaacatat ttagggtttg actcagggga agataacgta
     3361 gctgctaaaa aagtaggtct atatgactat gaaaaattgg ttactgaggc tggtgatgag
     3421 gctacagatg ttgctaaacg ctatgataaa tacgctgcag cccaagcttg gttgacagat
     3481 agtgctttga ttattccaac tacatctcgt acagggcgtc caatcttgtc taagatggta
     3541 ccatttacaa taccatttgc attgtcagga aataaaggta caagtgaacc agtcttgtat
     3601 aaatacttgg aacttcaaga caaggcagtc actgtagatg aataccaaaa agctcaggaa
     3661 aaatggatga aagaaaaaga agagtctaat aaaaaggctc aagaagatct cgcaaaacat
     3721 gtgaaataac tgttgcaaaa tataagaaag gatttagtat ttctcttgaa tgctgaatcc
     3781 ttttttacat ttgtaaagaa agattctaaa tgtactgacc cccaaaagtt ggagcctctt
     3841 tttgtcagaa tagagaaaat ttttgttaat tttacttgtt tcctattgct ttctcagcta
     3901 ttgtttgtta tattaaaagc ataattattt tttatttatc agggttaagc attgcacttt
     3961 cagaggaagg agtatttttt aaaaagaaaa tgtaaacgct tgctcaaaaa tgaaaggatt
     4021 tagaagttta tgaataaagg attatttgaa aaacgttgta aatatagtat tcggaaattt
     4081 tcattaggtg ttgcttctgt tatgattgga gctgcattct ttgggacaag tccggttctt
     4141 gcagatagcg tgcagtctgg ttccacggcg aacttaccag ctgatttagc tactgctctt
     4201 gcaacagcaa aagagaatga tggacgtgat tttgaagcgc ctaaggtggg agaagaccaa
     4261 ggttctccag aagttacaga tggacctaag acagaagaag aactattagc acttgaaaaa
     4321 gaaaaaacgg ctgaagaaaa accaaaagag gataaacctg cagctgctaa acctgaaacg
     4381 cctaagacgg taacccctga atggcaaacg gtagagaaaa aagaacaaca gggaacagtc
     4441 actatccgag aagaaaaagg tgtccgctac aaccaactat cctcaactgc tcaaaatgat
     4501 aacgcaggta aaccagccct gtttgaaaag aagggcttga ccgttgatgc caatggaaat
     4561 gcaactgttg atttaacctt caaagaagat tctgaaaagg gcaaatcacg ctttggtgtc
     4621 ttcttgaaat ttaaagatac caataataat gtttttgtcg gttatgacaa ggatggctgg
     4681 ttctgggagt ataaatctcc aacaactagc acttggtata gaggtagtcg tgttgctgct
     4741 cctgaaacag gatcaacaaa ccgtctctct atcactctca agtcagacgg tcagctaaat
     4801 gccagcaata acgatgtcaa tctctttgac acagtgactc taccagctgc ggtcaatgac
     4861 catcttaaaa atgagaagaa gattcttctc aaggcgggct cttatgacga tgagcgaaca
     4921 gttgttagcg ttaaaacgga taaccaagag ggggtaaaaa cagaggatac ccctgctgaa
     4981 aaagaaacag gtcctgaagt tgatgatagc aaggtgactt atgacacgat tcagtctaag
     5041 gtcctcaaag cagtgattga ccaagccttc cctcgtgtca aggaatacag cttgaacggg
     5101 catactttgc caggacaggt gcaacagttc aaccaagtct ttatcaataa ccaccgaatc
     5161 acccctgaag tcacttataa gaaaatcaat gagacaacag cagagtactt gatgaagctt
     5221 cgcgatgatg ctcacttaat caatgcggaa atgacagtac gcttgcaagt tgtggacaat
     5281 caattgcact ttgatgtgac caagattgtc aaccacaatc aagtcactcc aggtcaaaag
     5341 attgatgacg aaagaaaact actttcttct attagtttcc tcggcaatgc tttagtctct
     5401 gtttctagtg atcaaactgg tgctaagttt gatggggcaa ctatgtcaaa caatacgcat
     5461 gtcagcggag atgatcatat cgatgtaacc aatccaatga aggatttggc taagggttac
     5521 atgtatggat ttgtttctac agataagctt gctgctggtg tttggagtaa ctctcaaaac
     5581 agctatggtg gtggttcgaa tgactggact cgtttgacag cttataaaga aacagtcgga
     5641 aatgccaact atgtaggaat ccacagctct gaatggcaat gggaaaaagc ttataagggc
     5701 attgttttcc cagaatacac gaaggaactt ccgagtgcta aggttgttat cactgaagat
     5761 gccaatgcag acaagaaagt tgattggcaa gatggtgcca ttgcttatcg tagcattatg
     5821 aacaaccctc aaggttggga aaaagttaag gatatcacag cttaccgtat cgcgatgaac
     5881 tttggttctc aagcacaaaa cccattcctt atgaccttgg atggtatcaa gaaaatcaat
     5941 ctccacacag atggtcttgg gcaaggtgtt ctccttaaag gatatggtag cgaaggccat
     6001 gactctggtc acttgaacta tgctgatatt ggtaagcgta tcggtggtgt cgaagacttc
     6061 aagaccctaa ttgagaaggc taagaaatat ggagctcatc taggtatcca cgttaacgct
     6121 tcagaaactt atcctgagtc taaatacttc aatgaaaaaa ttctccgtaa gaatccagat
     6181 ggaagctata gctatggttg gaactggcta gatcaaggta tcaacattga tgctgcctat
     6241 gacctagctc atggtcgttt ggcacgttgg gaagatttga agaaaaaact tggtgacggt
     6301 ctcgacttta tctatgtgga cgtttggggt aatggtcaat caggtgataa cggtgcctgg
     6361 gctacccacg ttcttgctaa agaaattaac aaacaaggct ggcgctttgc gatcgagtgg
     6421 ggccatggtg gtgagtacga ctctaccttc catcactggg cagctgactt gacctacggt
     6481 ggctacacca ataaaggtat caacagtgcc atcacccgct ttatccgtaa ccaccaaaaa
     6541 gatgcttggg ttggggacta cagaagttat ggtggtgcag ccaactatcc actgctaggt
     6601 ggctacagca tgaaagactt tgaaggctgg caaggaagaa gtgactacaa tggctatgta
     6661 accaacttat ttgcccatga cgtcatgacc aagtatttcc aacacttcac tgtaagtaaa
     6721 tgggaaaatg gtacaccggt gactatgacc gataacggta gcacctataa atggactcca
     6781 gaaatgcgag tggaattggt agatgctgac aataataaag tagttgtaac tcgtaagtca
     6841 aatgatgtca atagtccaca atatcgcgaa cgtacagtaa ctctcaacgg acgtgtcatc
     6901 caagatggtt cagcttactt gactccttgg aactgggatg caaatggtaa gaaactttct
     6961 actgagaagg aaaagatgta ctacttcaat acgcaggccg gtgcaacaac ttggaccctt
     7021 ccaagcgatt gggcaaagag caaggtttac ctttacaagc taactgacca aggtaagaca
     7081 gaagagcaag aactaactgt aaaagatggt aaaattaccc tagatcttct agcaaatcaa
     7141 ccatacgttc tctatcgttc gaaacaaacc aatcctgaaa tgtcatggag cgaaggcatg
     7201 cacatctatg accaaggatt taatagtgga accttgaaac actggaccat ttctggtgat
     7261 gcttctaagg cagaaattgt caagtctcaa ggggcaaacg atatgcttcg tattcaagga
     7321 aacaaagaaa aagttagtct cactcagaaa ttaactggct tgaaaccaaa tactaagtat
     7381 gccgtttatg tcggtgtcga taaccgtagt aatgccaagg caagtatcac tgtgaatact
     7441 ggtgaaaaag aagtgactac ttataccaat aagtctctcg ccctcaacta tgttaaggct
     7501 tacgcccaca atacacgtcg tgacaatgct acagttgacg atacaagtta cttccaaaac
     7561 atgtacgcct tctttacaac tggatcggac gtatcaaatg ttactctgac attgagtcgt
     7621 gaagctggtg atcaagcaac ttactttgat gaaattcgta cctttgaaaa caattcaagc
     7681 atgtacggag acaagcatga tacaggtaaa ggcaccttca agcaagactt tgaaaatgtt
     7741 gctcagggta tcttcccatt tgtagtgggt ggtgtcgaag gtgtcgaaga caaccgcact
     7801 cacttgtctg aaaaacacga tccatataca caacgtggtt ggaatggtaa gaaagtcgat
     7861 gatgttatcg aaggaaattg gtcactcaag acaaatggac tagtgagccg tcgtaacttg
     7921 gtttaccaaa ccatcccaca aaacttccgt tttgaagcag gtaagaccta ccgtgtaacc
     7981 tttgaatacg aagcaggttc agacaatacc tatgcttttg tagtcggtaa gggagaattc
     8041 cagtcaggtc gtcgtggtac tcaagcaagc aacttggaaa tgcatgaatt gccaaatact
     8101 tggacagatt ctaagaaagc caagaaggca accttccttg tgacaggtgc agaaacaggg
     8161 gatacttggg taggtatcta ctcaactggc aatgcaagta atactcgtgg tgattctggt
     8221 ggaaatgcca acttccgtgg ttataacgac ttcatgatgg ataatcttca aatcgaagaa
     8281 attaccctaa caggtaagat gttgacagaa aatgctctga agaactactt gccaacggtt
     8341 gccatgacta actacaccaa agagtctatg gatgctttga aagaggcggt ctttaacctc
     8401 agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg cagagattgc caagattgaa
     8461 gccttgaaga atgctttggt tcagaagaaa acgactttgg tagcagaaga ctttgcaagt
     8521 ttggatgcgc cagctcaggc tcaagaaggt cttgcaaatg cctttgatgg aaacttatct
     8581 agtttatggc atacatcatg gggcggagga gatgtaggca agcctgcaac catggtcttg
     8641 aaagaagcaa ctgaaatcac tggacttcgt tatgttccac gtggatcagg ttcaaatggt
     8701 aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg gcaaggagca tacctttact
     8761 gcaactgatt ggccagataa caataagcca aaagacattg attttggtaa gacaattaag
     8821 gctaagaaaa ttgtccttac aggtactaag acttacggag atggtggcga taagtaccaa
     8881 tctgcagcgg aactcatctt tactcgtcca caggtagcag aaacacctct tgacttgtca
     8941 ggctatgaag cagctttggc taaggctcag aaattaacag acaaagacaa tcaagaggaa
     9001 gtagctagcg ttcaggcaag catgaaatat gcgacgaata accatctctt gacggaaaga
     9061 atggtggaat actttgcaga ttatctcaac caattaaaag attctgctac gaaaccagat
     9121 gctccaactg tagagaaacc tgagtttaaa cttagctctt tagcttccga tcaaggtaaa
     9181 acgccagatt ataagcaaga aatagctaga ccagaaacac ctgaacaaat cttgccagca
     9241 acaggtgaga gtcaatctga cacagccctc ttcctagcag gtgttagcct agccctatct
     9301 gctctctttg tagtaaaaac gaagaaagac tagtatttag taaaacctct taacaagatt
     9361 acggaagcag tctctatctt ttccaatgag gtttatagta cagaaaaagc ctgagaagat
     9421 gtcttctcag gcttttgtta agcacataaa tacaatagtg ctatgacaaa atcacccaga
     9481 aaaatctggg tgataaatgt tatggttgtg ctggttgagg attctgattt tgttgatcag
     9541 gggttgtatt tgattgttgc gtattgttgt taggattggt agtcgtacta ttatttgtgc
     9601 ttggagtggt taagctagac tgtgaagttg aactatctga tgatgagctt gaactttcag
     9661 ttgatggggg ttgttgtgga gcaggggagc tccacgtaga acgagcacca tttttaaata
     9721 cgaattctcc acttctgtat actccctcag gcattgtcca atctccagga tggtcatctt
     9781 ctgatagata cgatatcatt gagcgataaa ctttggcagc aactaggaaa ccatctccaa
     9841 tgattggagt taaacgattt gagtatccag tccatacagc catagaatac ttacgagtat
     9901 aacccacaaa catttcatct ggagctacgt agccagtgtt cttgatatac ttttcaattt
     9961 cgtcgtcagt atagttagaa gtacctgtct tacctgcttg tggaagccat ggtaggtagg
    10021 ctccacgtcc tgttccgtaa gttaaaacag ttttcatcat ttcagtcatc atataggcag
    10081 tagtctcttt catagctcgt gtaccagcat cagaaaattc tttttcgcta ccatcactaa
    10141 agacaacttt attgatgtac attggtttgt ggtaaatacc gccattagcg aatgcagcgt
    10201 aagccgcagc cattttctca ctgcttgccc cgtactgttt actagattca gttgtattac
    10261 ttgaaatagc atttgagtag tgaatacttg gatagtcaat tcccaaacca tttaggaaag
    10321 ttttggctct atctaaacca accttgttta gtgtttcaac ggcaggcacg ttacgagatt
    10381 gttgaagggc atattgcagg ctaatattac caaaatatgc tcgatcccag ttgtagacag
    10441 gtgtgcttgt gcccggatag ttataaggaa tatcattaac catagttgca gtggaatcat
    10501 atacaccata ttctaaggct ggggcataat cagtgattgg tttcatgctt gatccccagt
    10561 cacggttggt ttccacagct tggttggtac caaatgaaac gttacttgct tggtgacgag
    10621 ctccaagttg ggcgatgact ttaccatttg aaacatctac gaccgtagat gcgacttgca
    10681 aatcatcgtc agggtaagag acgtattgat cggagttgta gatatcccac agacgttttt
    10741 gagcctcttg gtctacattt gtgtagacat ccatcccagt agttagaagg ttatagccag
    10801 tttcttgttc tacttggtcg ataacctctt tgagataatt gtccatatat actgggtagc
    10861 tattgactga tttcaaactt tggagtccat cagtaatagg agtattgata gccttctcat
    10921 actgttcggc agaaatgtag ccttgacctt tcatctctga aagtaccaag ttacgacggt
    10981 caagggcagc ttctggatga gaatagggat cgtattgatt cggagcctgt ggcattcctg
    11041 ctagtagggc aagttgaggt agtgatagtt ctctcaaatc tttgccgtaa tagttttggg
    11101 cagccgtctg cattccatag ttgccgttag acatgtaaac tttattcacg tagtaggtca
    11161 gaatttcttg ttttgtggtt ttttgttcta attgaacagc caaccaagct tcctgggctt
    11221 tacgtgaaat ggtctgatca gaagtggaag ttgagaaata ggtcaactta attaattgtt
    11281 gggtaagggt tgatcctccc tggagagagt tgttttgtaa gttacgtaaa aaagctccta
    11341 taattcggat ggtatcgaca cctcgatgat cgaaaaaacg atggtcttcg attgatacga
    11401 tagccttgac caaatcagta ggaatttcgt tggcctgagc gtttactcgg cgttcagagc
    11461 ccagatcggc aatcagttcg tttttactat cgaaaatctt actagacgtg gttgcgacta
    11521 atttgctctc agatagggct ggggctttac tgacgtaata gagaaatatg cctcctccca
    11581 gcataactgc tgtgataaat aaagttaaga agcagatact cacatactta gctattcgca
    11641 ggatagtttg tttgttcatc ttgttttacc acctaataaa tgttctttga taacattgag
    11701 atagggaatt tgagggaagg caccagcctt gatttcatat ccatattctc gaatatatcc
    11761 aagtggcatt gatttttgtc ccttatcttg atggtagaag cgaatcaaat cgaatgccgg
    11821 caataagtag gtttcttgct gagaagaaaa gtgaagaagg acaaagcaga ttccttgttg
    11881 ggcaaggact tgttccatat gctgaatttg atgtggatga aaatttttca tcggaatcgc
    11941 acgtttttgt tttgtttcct tggcttcaaa gtcgatgtaa tatccattat aaacgccaga
    12001 atagtccgtc gttgaagctt gtcgaaaata ggcctcaaca atcttggcac gacttcgctg
    12061 tggatagtcc actcgtacaa tttgaatagg ggttggtttc ttgtgtataa cagccaagcc
    12121 ctgagacaaa tagtagtcgt tggtagcatt gatcatcttt tcaaaagaca ttcctcgatt
    12181 tgcgaaattt ttgggttgag aaagagatgt ttgtcttttt tgtgatgaaa gtttatgtgg
    12241 atagttgacc ataattctcc ttattggtac aataacatca ctctattata ccataaattt
    12301 gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc ttttgatttg
    12361 ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat acgtaaagat
    12421 ttagaggcta tggcagcaga tggggtgtct tggcttgtat ttacagggac tttgggcttt
    12481 gaatattggg ttttagaggt tgctcaggag atgaaaaccg agtacggttt ccagttggcc
    12541 accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca gatgaaactg
    12601 agtcgtttta agcaggtaga ctttgtcaaa tatgcttatc ctcgctatga gcacaagggg
    12661 cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta tcttttttat
    12721 gatgaagaaa atgaaacgaa actagcttat ttttaccaaa agatgaaaaa tcaagaggac
    12781 tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa tttttccgaa
    12841 aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata ctagcaagcg
    12901 agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt tgaacaagag
    12961 tttggacgtg aagtccgagg gtatagtaag gtagaagttg acgagttttt agacgatgtc
    13021 atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga aattgcagat
    13081 ttgaaggaag aattagcccg taaaccgcaa gtgagttcag ccccaagtcc tagtcaccca
    13141 gatccaattg atgtggcagc ttcatcttct atgacgaatt ttgatatttt gaaacgcttg
    13201 aatcgtcttg aaaaagaagt attcggcaaa caaattttag acaatcctga tttgtaattc
    13261 aggtcttgtc acatgcaatt tttggataat cgcgtgagga gagttgcttc tcatgaggaa
    13321 agtccatgct agcacaggct gtgatgcctg tagtgtttgt gctaggcgaa accataagcc
    13381 tagggacgag taatcgttac ggcagttgaa atggctaagt cctcggatag gtcagagtag
    13441 gcttgaaagt gccacagtga cggagtcttt ctggaaacgg agagagtgga acgcggtaaa
    13501 cccctcaagc tagcaaccca aattttggtc ggggcatgga gtacgcggaa acgaacgtag
    13561 tattctgact gctatcagct agagttgtta gtggtagaca gatgattatc gaaggaagtg
    13621 gtcctagtca cttctggaac aaaacatggc ttatagaaaa ttgcatatag gttggggctg
    13681 agaaaacttt ctcaacctca ttttttaaag tggacatata gaaaggtctt ataagactga
    13741 aaatgaaaaa agaatttaat ttaatcgcta ctgctgcagc gggtcttgag gctgtcgttg
    13801 gtcgtgaagt gcgagagttg ggctacgatt gtcaggttga aaatggacgt gttcgttttc
    13861 aaggagacgt gagaactatt atcgaaacca acctctggct tcgggcagca gatcgtatca
    13921 aaatcatcgt aggaaccttc caagctaaga cttttgaaga gctgtttcag ggagttttcg
    13981 ctctagattg ggaaaattat ttaccactcg gagctcggtt cccgatttca aaagctaaat
    14041 gtgttaagtc caaacttcac aatgagccca gtgttcaggc tatttctaag aaagctgttg
    14101 tcaagaaatt gcagaaacac tatgctcgcc cagaaggggt tcctctgatg gagaatggcc
    14161 cagagtttaa gattgaggtc tctattctca aagatgtggc aactgtcatg attgatacga
    14221 ccgggtctag cctctttaaa cgtggttatc gtaccgaaaa aggtggcgct cctatcaagg
    14281 aaaatatggc aacagccatt ttacaacttt ctaactggta tccagacaag cctttgattg
    14341 atccgacctg tggttcgggg actttctgta ttgaggcagt tatgattgct agaaagatgg
    14401 cgccaggtct tcgtcgctct tttgcatttg aggaatggaa ctggatcagc gatcgcttga
    14461 ttcaagaagt gcgcacagaa gcggctaaaa aagtagaccg tgagcttgag ctggatatca
    14521 tgggctgtga tattgatgct cgcatggtgg aaattgctaa ggccaatgct caggtagctg
    14581 gtgttgcagg agacattact tttaagcaga tgcgcgtgca ggatttacgt tccgataaaa
    14641 tcaatggagt aatcatttcc aatccgcctt atggtgaacg tttgtcagat gatgcagggg
    14701 tgaccaagct ctatgctgag atggggcaag tatttgcacc gctgaaaact tggagcaaat
    14761 ttatcctgac tagtgatgaa gcttttgaaa gcaagtatgg tagccaagca gataagaagc
    14821 gtaagttata caacggaacc ttgaaagtgg atctatatca atattttggt cagcgtgtca
    14881 aacggcaaga ggtaaaatag aaagggatac tcatgagtaa aaaaagacga aatcgtcata
    14941 aaaaagaagc tcaagaaccg caatttgatt ttgatgaagc aaaagagcta acagttggtc
    15001 aagctattcg taaaaatgaa gaagtggaag caggagtctt gcctgaggat tccattttgg
    15061 acaagtatgt taagcaacac agagatgaaa ttgaggcgga taagtttgcg actcgtcaat
    15121 acaaaaaaga ggagttcgtt gaaactcaga gtctggatga tttaattcaa gagatgcgtg
    15181 aggctgtaga gaagtcagaa gcttcttcgg aggaagttcc atcttctgaa gacatcttac
    15241 tacccttgcc tctggacgat gaggagcaag gcttggatcc tctattgcta gatgatgaaa
    15301 atccaacaga aatgactgaa gaagtggaag aggagcaaaa cctttctcat ctggatcaag
    15361 aggactcaga aaagaaaagt aaaaaaggct ttattttgac cgttttggcg cttgtatcag
    15421 taattatttg tgtcagtgct tattatgtct accgtcaagt ggctcgttcg accaaggaaa
    15481 ttgaaacttc tcaatcaact acagccaatc aatcggatgt ggatgatttt aatacacttt
    15541 atgacgcctt ttacacagat agcaataaaa cggctttgaa aaatagccag tttgataaac
    15601 tgagtcaact caaaaccttg cttgataaac tggaaggtag tcgtgaacat acgcttgcca
    15661 aatctaaata tgatagtcta gcaacgcaaa tcaaggctat tcaagatgtc aatgcacaat
    15721 ttgaaaaatc agctattgtg gatggtgtgt tggataccaa tgccaaagcc aaatcggatg
    15781 ctaaatttac agatattaaa actggaaata cggagcttga taaagtgcta gataaggcta
    15841 tcagtcttgg taagagccag caaacaagta cttctagctc aagtttaagt caaactagca
    15901 gctcaagttc aagtcaagca agttcaaata cgactagtga gccaaaacca agtagttcaa
    15961 atgagactag aagtagtcgc agtgaagtca atatgggtct ctcgagtgca ggggttgctg
    16021 ttcaaagaag tgccagtcgt gttgcctata atcagtctgc tattgatgat agtaataact
    16081 ctgcctggga ttttgcggat ggtgtcttgg aacaaattct agcgacttca cgttcacgtg
    16141 gctatatcac tggagaccaa tatatccttg aacgtgtcaa tatcgttaac ggcaatggtt
    16201 attataacct ctataagcca gatggaacct atctctttac ccttaactgt aagacaggct
    16261 actttgtcgg aaatggcgct ggtcatgcgg atgacttgga ctactaagca gtcgttacaa
    16321 aattctttct tttcaaaagt aaaaatgata aaataaaaca aatgaaacaa gaggagtgtc
    16381 aaatgacaaa agctaacttt ggtgtcgtag gtatggccgt aatgggtcgt aaccttgccc
    16441 ttaatattga atcacgtggt tacacagttg ctatctacaa ccgtagtaaa gaaaaaacgg
    16501 aagatgtgat tgcttgccat cctgaaaaga actttgtacc aagctatgac gttgaaagtt
    16561 ttgtaaactc aatcgaaaaa cctcgtcgta tcatgctgat ggttcaagct ggacctggta
    16621 cagatgctac tatccaagcc cttcttccac accttgacaa gggtgatatc ttgattgacg
    16681 gtggaaatac tttctacaaa gataccatcc gtcgtaatga agaattggca aactcaggta
    16741 tcaactttat cggtactgga gtttctggtg gtgaaaaagg tgcccttgaa ggtccttcta
    16801 tcatgcctgg tggacaaaaa gaggcctacg aattggttgc ggatgttctt gaagaaatct
    16861 cagctaaagc accagaagat ggcaaaccat gtgtgactta tatcggtcct gatggagctg
    16921 gtcactatgt gaaaatggtt cacaacggta tcgagtacgg tgatatgcaa ttgatcgcag
    16981 aaagctatga cttgatgcaa cacttgctag gcctttctgc agaggatatg gctgaaatct
    17041 ttactgagtg gaacaagggt gaattggata gctacttgat cgaaatcaca gctgatatct
    17101 tgagccgtaa agacgatgaa gaccaagatg gaccaatcgt agactacatc cttgatgctg
    17161 caggtaacaa gggaactggt aaatggacta gccaatcatc tcttgacctt ggtgtaccat
    17221 tgtcactgat tactgagtca gtgtttgcac gctacatttc aacttacaaa gaagaacgtg
    17281 tacatgctag caaggtgctt ccaaaaccag ctgccttcaa ctttgaagga gacaaggctg
    17341 aattgattga aaaaatccgt caagcccttt acttctcaaa aatcatttca tacgcacaag
    17401 gatttgctca attgcgtgta gcctctaaag aaaacaactg gaacttgcca tttgcagata
    17461 tcgcatctat ctggcgtgat ggctgtatca tccgttctcg tttcttgcaa aagattacag
    17521 atgcttacaa ccgcgatgca gatcttgcca accttctttt ggacgagtac ttcttggatg
    17581 ttactgctaa gtatcaacaa gcagtgcgtg atatcgtagc tcttgcggtt caagctggtg
    17641 tgccagtgcc aactttctca gcagctatta cttactttga tagctaccgt tcagctgacc
    17701 ttccggctaa cttgatccaa gcacaacgtg actactttgg tgctcacact taccaacgta
    17761 aagacaaaga aggaaccttc cactactctt ggtatgacga aaaataagta ggtcagccat
    17821 ggggaaacgg attttattac ttgagaaaga acgaaatcta gctcattttt taagtttgga
    17881 actccagaaa gagcagtatc gggttgatct ggtagaggag gggcaaaaag ccctctccat
    17941 ggctcttcag acagactatg atttgatgtt attgaacgtt aatctgggag atatgatggc
    18001 tcaggatttt gcagaaaaat tgagtcgaac taaacctgcc tcagtcatca tgattttaga
    18061 tcattgggaa gacttgcaag aagagctgga agttgttcag cgttttgcag tttcatacat
    18121 ttataagcca gtccttatcg aaaatctggt agcgcgtatt tcggcgatct tccgaggtcg
    18181 ggacttcatt gatcaacact gcagtctgat gaaagttcca aggacctacc gcaatcttag
    18241 gatagatgtt gaacatcaca cggtttatcg tggtgaagag atgattgctc tgacacgccg
    18301 tgagtatgac cttttggcga cacttatggg aagcaagaaa gtattgactc gtgagcaatt
    18361 gttggaaagt gtttggaagt atgaaagtgc gaccgagaca aatatcgtag atgtctatat
    18421 ccgctatcta cggagcaagc ttgatgttaa aggccaaaaa agctacatta aaactgtgcg
    18481 tggtgttgga tataccatgc aagaatagaa aagcagttgc agttgtgtaa ctgctttttt
    18541 gaggagtttt tatatattga catacaatct agtttttact acaatcgttt taggaggata
    18601 tataaaatga agcttttgaa aaaaatgatg caagtcgcat tagcagtctt tttctttggt
    18661 ttgctagcta caaatacggt atttgcgaat accacaggtg gccgatttgt tgataaggat
    18721 aatagaaaat attatgtaaa agatgatcat aaagcaatct attggcataa aatagacggt
    18781 aaaacttact attttggtga tattggagag atggttgtcg gttggcaata cttagaaatt
    18841 cctggaacag gttatcgtga taatttattc gataaccaac cagttaatga aattggcctt
    18901 caggagaagt ggtactattt tggacaagat ggtgctttgc tagaacaaac agataaacaa
    18961 gtactagagg caaaaacgtc tgaaaataca ggaaaagtat acggtgaaca atatcctcta
    19021 tctgctgaaa agagaactta ttattttgat aataattatg ctgtaaagac aggctggatt
    19081 tatgaagagg gtcattggta ttatttaaat aagctaggaa attttggcga tgattcttac
    19141 aatccactac caattggtga agttgctaag ggttggactc aagattttca tgttactatt
    19201 gacattgata gaagcaaacc tgctccatgg tactacctag atgcttcagg taagatgctt
    19261 acagattggc aaaaagtaaa cggaaaatgg tattattttg gctcctctgg ttctatggca
    19321 acaggttgga aatatgtacg aggcaaatgg tattacttag ataataaaaa tggtgatatg
    19381 aaaacaggat ggcaatacct tggtaacaag tggtactacc tccgttcatc aggagctatg
    19441 gtaactggct ggtatcaaga tggttcaact tggtactatt tagatgctga aaatggtgat
    19501 atgaaaatag gttggacaaa agtaaatgga aaatggtatt atctcaattc ctctggagca
    19561 atggttacag gtagtcaaac tatcgatggt aaagtttata acttcgcttc atctggtgag
    19621 tggatttaat gttggaggat atataaaatg aagcttttga aaaaaactat gcaagctgga
    19681 ctaacagtaa ttttcttcgg tttgctaggg accagtacag tatttgcaga tgattctgaa
    19741 ggatggcagt ttgtccaaga aaacggtaga acctattaca aaaaggggga cctaaaagaa
    19801 acctactgga gagtgataga tgggaagtac tattattttg attctctatc tggagagatg
    19861 gttgtcggct ggcaatatat cccgtttcca tctaaaggta gtacaattgg tccttaccca
    19921 aatggtatca gattaaaagg ttttccaaag tcagagtggt actacttcga taaaaatgga
    19981 gtgctacaag agtttgttgg ttggaaaaca ttagagatta aaactaaata cagtgttgga
    20041 agaaagtacg gggaaaaacg tgaagattca gaagataaag aagagaaacg ttattatacg
    20101 aactattact ttaatcaaaa tcattcttta gagacaggtt ggctttatga tcagtctaac
    20161 tggtattatc tagctaagac ggaaattaat ggagaaaact accttggtgg tgaaagacgt
    20221 gcggggtgga taaacgatga ttcgacttgg tactatttag atccaacaac tggtattatg
    20281 caaacaggtt ggcaatatct aggtaataag tggtactacc tccgttcatc aggattaatg
    20341 gcaactggct ggtatcagga aggtaccact tggtattatt tagaccagtc aaatggagat
    20401 atgaaaacag gctggcaata ccttggtaac aagtggtact accttcgttc atcaggagca
    20461 atggcaactg gttggtatca agatggttca acttggtact acctaaatgc aggtaatgga
    20521 gatatgaaga caggttggtt ccaggtcaat ggcaactggt actatgctta tagctcaggt
    20581 gctttggcag tgaatacgac cgtagatggc tattctgtca actataatgg cgaatgggtt
    20641 cggtaatgac agggcgctta taattatatt agaacactcg cttaatcaaa gaagtgactt
    20701 tgttgtactt gacctgacga ttggggagat taaaagcatg tgaaatctac acatgctttt
    20761 tcgttagtta tgagtgttga acttgactaa ccattgacaa aagataagag caattaaaat
    20821 aagatacaaa aatatcaatg ctaactgcac cgaaacgccc gatgctatgg atagggctaa
    20881 tagggagagc agggacgggg cgagaatgtc cactgtgctc agagcagagc ccaccgtccc
    20941 catcttgtca ctggggatat gggcaaagac agctgcttgt aggcgagggc tgagaatgcc
    21001 gataatcgta gaactgatga aactggtcat gaaaattacg atataggctt gacaaaggat
    21061 actagttatt agagtcaata gggaaatctc acagaaaacc aagaggtgcg tgaaacggat
    21121 atttttaaat agattactgc tggtcatatt gccaaggata gctccacaag acatgatgat
    21181 ggagagcagg gcgaggagtt gcccaatgct cagttgtgct agtgtctgtt ggatgaagcg
    21241 gagggcaatc agccgagggg acacatccaa gatggcaatc atactggtcg tcagaaacag
    21301 tagtatgacg gtatctttta acctcaggag cacagccagc gactgtttaa gatgttgaaa
    21361 atatttctta aaactcagtg ctgtatgtga catttcaatt cttttttcaa cctcatacaa
    21421 ggtatgtcga ataacataca ggcccaaaaa ggcaatgaca aaagtcagag tgttgataag
    21481 ggaaatagtt tgaatactta taacattgat aaggaatgcg ccagccagat tggcgacaat
    21541 acggacaacc ctcatcagag actgcctgaa ccccatggca tcatgcaggt cgtccttaat
    21601 tacactgatg tagagggcgt tcatcatgta ggcagacagg tagctcaagg tgtcagagat
    21661 gaggttgatg atgacaatca taatgactga aactaatgaa aagggcgaca agacgagaaa
    21721 tactgtcaaa atagcgtaga gcaggatttt gattttggca atccagagtg cgtgtttgac
    21781 tctattttct tgaaaatctc ctagaatccc gagaaagagt tgcgatagta ggggaaccat
    21841 ctctgaaatg gaaatcagta aaatcgctag cggagcgaaa ttgtactgag ccacataatt
    21901 gataatggcg aggtagaaaa tactgtcgcc gaatgatgaa aaacagctgt ttagcagcaa
    21961 ctgtctatat atgttgtttg tccaaaataa tttcatactt ttctccttat gtctgcacat
    22021 cgaatgggaa tttattattt aatagacacc aatatacccc atgcttccta ttccgaagtc
    22081 aaacaaatct gctttaccac tatctccttg aaacatcagt aaaccgtgat catctcgttc
    22141 agaacatgct aaaattacct gttggatttt tttcatcagt aatttgtttt gattgtagac
    22201 agtggtttgt agtaagctag ttattccgtg gcaatatcct gtcatcattt ttagatgatg
    22261 gttaaaaaca aattcttgat atttactaat aatatctttg tttccgtcac aatcgtacat
    22321 acaaagatat aagattattc cggaaattcc tttgcaccaa gaaagttgta aatttcctaa
    22381 attttcagta ttagaagtaa ctttttctaa taattttttt aattcagtat ggaatgtatg
    22441 gagatcatta tagaacatag aaggttctaa gactttagaa taggcaaata aagcatatgc
    22501 aattccacaa tatccatgag caaagctata gtctaaaatg ctttctttgg ctgtatctct
    22561 ttgtaggaaa taatgataaa tttcccctat actattgtgg atgaaaattt taagagtatt
    22621 gtcattggta aattgatagt attttattaa tgataatagg acaccagatt ttcccaatgc
    22681 aaagtctatt tcctctagag tatcataatt ctctattaat tttttagcgg tgacgtttgc
    22741 taattgtaga tagtattgat ttttactgat agaaaataaa tgtaggagta accatagata
    22801 gcctgcatca ccaaatagca gtgagtattg atattcatat gtatgtgtcg ttctaattgt
    22861 cactgccttc ttgatgatag agagtatttt agaatccagt tctggggggt acaatttatt
    22921 tagaggaaac agtagtccag ctattccgtg ttggaatgat aaattactta cgaaatttcc
    22981 aaattctgtt gaagaagtga tccttccttc agaatttaca gttttatcta gtaaaaattg
    23041 tagaacacta ttacattctg ttttgtaatc atatttatgt tcctctattt ttttaatttt
    23101 gactaaatta tttgtttgac ttaaatttag tagacattgt aaccaaaata gttgctcatc
    23161 tgtgagctga ccttgagtat gtgatagttc tagtaattga ataggatcct ttagttagat
    23221 tcaatcaatc tataacacaa caacataata atatttttag gataacttat caaatattat
    23281 agtaaaatga gataataaca ggataaatcg atc
//
LOCUS       DAVKJU010000028        23079 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVKJU010000028 DAVKJU010000000
VERSION     DAVKJU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.028"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            288..635
                     /gene="ssrA"
                     /locus_tag="VW191_001066"
     tmRNA           288..635
                     /gene="ssrA"
                     /locus_tag="VW191_001066"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(818..905)
                     /locus_tag="VW191_001067"
     tRNA            complement(818..905)
                     /locus_tag="VW191_001067"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Ser,seq:gga)
     gene            1068..1808
                     /locus_tag="VW191_001068"
     CDS             1068..1808
                     /locus_tag="VW191_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9623848.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1805..2716
                     /gene="pfkB"
                     /locus_tag="VW191_001069"
     CDS             1805..2716
                     /gene="pfkB"
                     /locus_tag="VW191_001069"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEU9623849.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2713..4665
                     /locus_tag="VW191_001070"
     CDS             2713..4665
                     /locus_tag="VW191_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9623850.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALTELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSND
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            4829..7054
                     /locus_tag="VW191_001071"
     CDS             4829..7054
                     /locus_tag="VW191_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU9623851.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7253..7678)
                     /locus_tag="VW191_001072"
     CDS             complement(7253..7678)
                     /locus_tag="VW191_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623852.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
     gene            7816..8958
                     /locus_tag="VW191_001073"
     CDS             7816..8958
                     /locus_tag="VW191_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9623853.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8967..10181
                     /gene="thiI"
                     /locus_tag="VW191_001074"
     CDS             8967..10181
                     /gene="thiI"
                     /locus_tag="VW191_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEU9623854.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEVTPQAE
                     KDEVDDLIDNLL"
     gene            10316..11140
                     /locus_tag="VW191_001075"
     CDS             10316..11140
                     /locus_tag="VW191_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9623855.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFYSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYTAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYIECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            11162..11902
                     /locus_tag="VW191_001076"
     CDS             11162..11902
                     /locus_tag="VW191_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9623856.1"
                     /translation="MHIEHLSHWSGHLNREMYLNRYGHGGIPVVVFASSGGSHNEYYD
                     FGMIDACASFIEEGLVQFFTLSSLDSESWLVTWKNAHDQAEMHRAYERYVIEEAIPFI
                     KHKTGWFDGMMTTGCSMGAYHALNFFLQHPDVFTKVIALSGVYDARFFVGDYYNDDAI
                     YQNSPVDYIWNQNDGWFIDRYRQAEIVLCTGLGAWEQDGLPSFYKIKEAFDQKQIPAW
                     FAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            11915..13081
                     /locus_tag="VW191_001077"
     CDS             11915..13081
                     /locus_tag="VW191_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9623857.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMVPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYIAIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            13589..15082
                     /locus_tag="VW191_001078"
     CDS             13589..15082
                     /locus_tag="VW191_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9623858.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            15095..16225
                     /locus_tag="VW191_001079"
     CDS             15095..16225
                     /locus_tag="VW191_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9623859.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKYL
                     RINSGMVILRPKTPNLNQKFIIHVLKFIIHVLRNNNYSRVISGSAQPQLPITKLKKIL
                     LPLPPLALQNEFADFVALVDKSQFACEIAIKVWRNSLKFSII"
     gene            16255..19605
                     /locus_tag="VW191_001080"
                     /pseudo
     CDS             16255..19605
                     /locus_tag="VW191_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            19876..19953
                     /locus_tag="VW191_001081"
                     /pseudo
     CDS             19876..19953
                     /locus_tag="VW191_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(20192..20662)
                     /locus_tag="VW191_001082"
     CDS             complement(20192..20662)
                     /locus_tag="VW191_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9623860.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(20679..22952)
                     /locus_tag="VW191_001083"
     CDS             complement(20679..22952)
                     /locus_tag="VW191_001083"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEU9623861.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
BASE COUNT         6997 a         4136 c         5038 g         6908 t
ORIGIN      
        1 tatagttttt aaactcaaat gaattaaaat aaagagagta cgaaaattct catctgaaag
       61 tattttagaa taattctctt cgtgaatttc ttcaaaacag atagcttcat cttaggtatg
      121 tgttaattgg atttcgtaag ctctatttac aatcgtaaag ttatgcactg aaaaagttgt
      181 agttagtctt ccagtttgct aaatttctcg aatagtatct tttttaggta tgtgatttct
      241 ttttgcattt ttgagttaga taaggtataa tggttttatt gtcttttggg gtcgttacgg
      301 attcgacagg cattatgagg catattttgc gactcgtgtg gcgacgtaaa cgctcagtta
      361 aatataactg caaaaaataa cacttcttac gctctagctg cctaaaaacc agcaggcgtg
      421 acccgatttg gattgctcgt gttcaatgac aggtcttatt attagcgaga tacgattaag
      481 ccttgtctag cggcttgata agagattgat agactcgcag tttctagact tgagttatgt
      541 gtcgaggggc tgttaaaata atacataacc tatggttgta gacaaatatg ttggcaggtg
      601 tttggacgtg ggttcgactc ccaccggctc cattattcct ttgcattctt ttgcattcct
      661 tggtaaaacg ttgttaaatc aacgtttttt atttttatct ttggtattcc tttgcattct
      721 tttgctaaaa agggagtcac aaacagaccc tattttaaaa aaaggataga aaaaaggata
      781 caacatttgt cgcatcctaa aaataatctt ttttcgacgg aagacatggg attcgaaccc
      841 acgcacgcta ttacacgcct accgcgtttc caacacggcc tcttaagcct cttgagtaat
      901 cttccaatac ttactcaaat agtctaccat aaaggctctt atcttgcaat aaaaattcta
      961 gaaataagaa aaaatgatag attttgaaag aaaatgataa aaaatgcttg acttcgaaag
     1021 aaagtatgat agaatgaata gtgtaaacga taacaggagg tgattcagtg ttaaaaacag
     1081 aacgtaaaca actaatttta gaggagttaa atcaacatca tgtagtttct ctagaaaaat
     1141 tagttagttt gctagaaacg tcagaatcaa cggttcgaag agacttggat gagttggaag
     1201 cggaaaacaa gcttcgtcgt gtgcatggtg gagcagaact cccccactcc ttacaggaag
     1261 aagaaaccat tcaagaaaaa tctgtcaaaa accttcaaga aaagaagttg ctggctcaga
     1321 aagcagcttc tctcattaaa gaaaaagatg tcatctttat cgatgctgga acaacaactg
     1381 cttttttgat tcatgaattg gtcaataaga atgttacagt tgtgaccaac tccattcacc
     1441 atgccgctca gttggttgaa aagcagattc caactgtcat ggttggagga aacgtcaaga
     1501 cggcgacaga tgctagtatc gggggcgttg ctcttaacca gattaaccaa ttgcactttg
     1561 accgtgcctt tatcggaatg aatggtgttg acgatggcta ttatacgact cctgatatgg
     1621 aggagggagc tgtgaaaaga gctattttgg agaatgccaa gcagacctac gtcttggtgg
     1681 attcgtcaaa aattggacaa acttgctttg ccaaggtagc accactcaaa cgcgctatcg
     1741 ttatcactag tcaagggcat gagctcttgc aggttattaa ggagaaaacg gaggtaatag
     1801 aagtatgatt tatacagtca cactcaatcc atccattgac tatatcgttc gtttggacca
     1861 agtcaaagtt ggtagtgtaa atcgtatgga cagtgatgat aagtttgctg gtgggaaagg
     1921 aatcaatgtc agccgtgtct tgaaacgttt gaatatacca aatacagcga cgggatttat
     1981 cggtggcttt actggtaaat ttatcacaga tactttagca gaggaagaaa tcgagacacg
     2041 ttttgtccag gtggcagaag atactcgtat caatgttaaa atcaaagcag accaagaaac
     2101 agaaatcaac ggaacgggtc caactgttga atcggttcag ctagaagaat tgaaagctat
     2161 tttatctagt ctgacagcag aagatacagt tgtctttgca ggttcaagtg ctaaaaatct
     2221 aggcaatgtt atctataagg atttgatttc cttgacgcgc cagactggtg cgcaagtggt
     2281 ctgtgacttt gaaggacaga ccttaattga tagtttggac taccagcctc ttcttgtaaa
     2341 accaaacaat catgaacttg gagcgatttt tggggttaaa ctcgaaagtt tagatgaaat
     2401 tgagaaatac gctcgtgagt tactggctaa gggtgctcaa aatgttatta tctctatggc
     2461 tggtgatggt gcccttcttg tcacatctga gggagcttac ttcgctaaac caatcaaagg
     2521 aacagtcaaa aattcagttg gagctggtga ttctatggtt gctggattca caggtgaatt
     2581 tgtcaaatca aaagacgtag tagaagcctt caaatgggga gtggcttgcg gaacggcaac
     2641 taccttctca gatgacttgg caacggcgga atttattaaa gaaacatatg gaaaagttga
     2701 ggtagaaaaa cgatgaaaat tcaagaccta ttgagaaaag atgtcatgtt gctagatttg
     2761 caggcaactg aaaaaacagc tgtcatcgac gagatgatta aaaatttgac agaccacggt
     2821 tatgtaacag attttgaaac atttaaagaa ggaattttgg cgcgtgaagc tttgacttct
     2881 actggtttgg gtgatggaat cgcaatgcct cacagcaaaa acgctgctgt caaagaagcg
     2941 acagttctat ttgctaagtc aaataagggt gttgactacg agagcttgga tggacaagca
     3001 actgacctct tcttcatgat tgcagctcca gaaggtgcca atgatactca cttggcagca
     3061 ttgacagaat tgtctcaata cttgatgaaa gacggttttg cagacaaact tcgtcaagca
     3121 acatctgcag accaagttat cgaacttttt gaccaagctt cagaaaaaac tgaggaactt
     3181 gttcaagcac ctgctaatga ctctggtgac tttatcgtag ctgttacagc ttgtacaaca
     3241 ggtattgccc acacttacat ggcccaagaa gcccttcaaa aagtagctgc tgaaatgggg
     3301 gttggtatca aggtcgaaac caacggtgct agcggtgttg gaaatcaact aactgcagaa
     3361 gatatccgta aggctaaagc tattatcatt gcagcagaca aggccgttga aatggatcga
     3421 tttgatggaa aaccattgat caatcgtcca gttgctgacg gtatccgtaa gacagaagag
     3481 ctaattaact tggctctttc aggagatact gaagtctacc gtgccgctaa tggtgcaaaa
     3541 gctgcaacag cctctaacga aaaacaaagc cttggtggtg ccttctacaa acacttgatg
     3601 agtggtgtat ctcaaatgtt accattcgtt atcggtggtg gtatcatgat tgcccttgcc
     3661 ttcttgattg acggtgcttt gggtgttcca aatgaaaacc ttggcaatct tggttcttac
     3721 catgagttag cttctatgtt catgaaaatt ggtggagctg cctttggttt gatgcttcca
     3781 gtctttgcgg gttatgttgc ctactctatt gctgaaaaac caggtttggt agcaggtttc
     3841 gtggctggtg ctattgccaa agaaggtttt gcctttggta aaattcctta tgccgcaggt
     3901 ggtgaagcaa cttcaactct tgcaggtgtc tcatctggtt tcctaggtgc ccttgttggt
     3961 ggatttatcg caggtgcctt ggttcttgcc atcaagaaat acgttaaagt tcctcgttca
     4021 ctcgaaggtg ctaaatcaat ccttctattg ccacttcttg gaacaatctt gacaggattt
     4081 gttatgctag ctgtgaatat cccaatggct gcaatcaaca ctgctatgaa tgacttccta
     4141 ggcggtcttg gaggaggttc agctgtcctt cttggtatcg tccttggtgg aatgatggct
     4201 gttgacatgg gtggaccagt taataaagca gcttatgtct ttggtacagg tacgcttgca
     4261 gcaactgttt cttcaggtgg ttctgtagcc atggcagcag ttatggctgg aggaatggtg
     4321 ccaccacttg caatctttgt cgcaactctt cttttcaaag ataaatttac taaggaagaa
     4381 cgtaactctg gtttgacaaa catcatcatg ggcttgtcat ttatcactga gggagcgatt
     4441 ccatttggtg ccgctgaccc agctcgtgcg attccaagct tcatccttgg ttcagcagta
     4501 gcaggtggac tcgttggtct tactggtatc aaactcatgg cgccacacgg aggaatcttc
     4561 gttatcgccc ttacttcaaa tgatctcctt tacctcgttt ctgtcttggt aggagcaatc
     4621 gtaagtggtg tggtttatgg ttacctacgc aaaccacaag cataaaaaat agaaaaatga
     4681 aaagattgga ccgtttggtg cagtcttttt ctcttcccga aatgcctgtg aaatatggta
     4741 taatagaaga atggcaaaca agaatacaag tacaacaaga cggagaccgt ctaaagcaga
     4801 actggaaaga aaagaagcga ttcaacgaat gttgatttcg ttaggaattg cgattttatt
     4861 gattttcgca gccttcaaat taggggctgc aggtataacc ctttataatt taattcgctt
     4921 gctagtgggt agcctagctt atctggcgat attcggccta ttaatctatc tcttcttttt
     4981 caagtggata cgaaaacagg aaggactctt atctggcttt ttcaccatat ttgctggctt
     5041 actcttgatt tttgaggcct acttggtttg gaaatatggt ttggacaagt ccgttctaaa
     5101 agggaccatg gctcaggttg tgacagatct gactggtttt cgaacgacta gttttgctgg
     5161 agggggcttg atcggggtcg ctctttatat tccaacagcc tttctctttt caaatatcgg
     5221 aacttacttt attggttcta ttttgatttt agtgggttct ctcttagtca gcccttggtc
     5281 tgtttacgat attgctgaat ttttcagtag aggctttgcc aaatggtggg aagggcacga
     5341 gcgtcgaaaa gaggaacgct ttgtcaaaca agaagaaaaa gctcgccaaa aggctgagaa
     5401 agaggctaga ttagaacaag aagagactga aaaagcctta ctcgatttgc ctcctgttga
     5461 tatggaaacg ggtgaaattc tgacagagga agctgttcaa aatcttccac ctattccaga
     5521 agaaaagtgg gtggaaccag aaatcatcct gcctcaagct gaacttaaat tccctgaaca
     5581 ggaagatgac tcagatgacg aagatgttca ggtcgatttt tcagccaaag aagcccttga
     5641 atacaaactt ccaagcttac aactctttgc accagataaa ccaaaagatc agtctaaaga
     5701 gaagaaaatt gtcagagaaa atatcaaaat cttagaagca acctttgcta gctttggtat
     5761 taaggtaaca gttgaacggg ccgaaattgg gccatcagtg accaagtatg aagtcaagcc
     5821 ggctgttggt gtaagggtca accgcatttc caatctatca gatgacctcg ctctagcctt
     5881 ggctgccaag gatgtccgga ttgaagcgcc aatccctggg aaatccctaa tcggaattga
     5941 agtgcccaac tccgatattg ccactgtatc tttccgagaa ctatgggaac aatcgcaaac
     6001 gaaagcagaa aatttcttgg aaattccttt agggaaggct gttaatggaa ccgcaagagc
     6061 ttttgacctt tctaaaatgc cccacttgct agttgcaggt tcaacgggtt cagggaagtc
     6121 agtagcagtt aacggcatta ttgctagcat tctcatgaag gcgagaccag atcaagttaa
     6181 atttatgatg gtcgatccca agatggttga gttatctgtt tacaatgata ttccccacct
     6241 cttgattcca gtcgtgacca atccacgcaa agccagcaag gctctgcaaa aggttgtgga
     6301 tgaaatggaa aaccgttatg aactctttgc caaggtggga gttcggaata ttgcaggttt
     6361 taatgccaag gtagaagagt tcaattccca gtctgagtac aagcaaattc cgctaccatt
     6421 cattgtcgtg attgtggatg agttggctga cctcatgatg gtggccagca aggaagtgga
     6481 agatgctatc atccgtcttg ggcagaaggc gcgtgctgca ggtatccaca tgattcttgc
     6541 aactcagcgt ccatctgttg atgtcatctc tggtttgatt aaggccaatg ttccatctcg
     6601 tgtagcattt gcggtttcat caggaacaga ctcccgtacg attttggatg aaaatggagc
     6661 agaaaaactt cttggtcgag gagacatgct ctttaaaccg attgatgaaa atcatccagt
     6721 tcgtctccaa ggctccttta tctcggatga cgatgttgag cgcattgtga acttcatcaa
     6781 gactcaggca gatgcagact acgatgagag ttttgatcca ggtgaggttt ctgaaaatga
     6841 aggagaattt tcggatggag atgctggtgg tgatccgctt tttgaagaag ctaagtcttt
     6901 ggttatcgaa acacagaaag ccagtgcatc catgattcag cgtcgtttgt cagttggatt
     6961 taaccgtgcg acccgtctca tggaagagct tgagatggca ggtgtcattg gtccagctga
     7021 aggtaccaag ccaagaaaag tattgcagca ataaaaaata gcttctttcc aaggttggaa
     7081 ggagctattt tactggctat tgattacttt tatactcttc gcaaatctct tcaaaccacg
     7141 tcagctttgc cttgccgtag gtatatgata ctgactctgt cagttctatc cacaacctca
     7201 aaacagtgtt ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag
     7261 tattattttc tgaagttgga gtattgggct gtttttcatt ttcagtggca ggtttacttg
     7321 tagcaggtgt agtagagtcc tgagttgctg ttttctgatc ttcttttttc tcttccttga
     7381 cgctagattt tggcgtttct tcttgttgtg ttttttcttg agaagtgtta ttttccttat
     7441 ttggagtagt attttcctga ggggattctt tttgaatttc tttgacaaag gttgtcgtct
     7501 ggcttgtcgt aggttctttt ttagtatttt tattattatc caaggcgttc atgatcgtga
     7561 tacccgctgt aatcaaacca aggatactac cgatagtagt aatggttttt tgaaaaacag
     7621 taaagccttt tttgatatgt tctgttttcg gttttgaagt tctacgatag ttagacatac
     7681 attctctcct ttgattatga tttattatac tctgtttctt taaaaaaatc ttaaaaaaag
     7741 agaaattgcc ctttcttgtc gcaagctccg tttcatgata caatagaggg agtgatttta
     7801 gaaagcgtga gaaacatgat ttactttgat aattcagcga cgaccaagcc ttatcctgaa
     7861 gcccttgaaa cctatatgca ggtcgcttca aaaattttag gaaacccatc tagtctccat
     7921 cgtttgggag accaagcaac acgaatctta gatgcttctc gccaacagat tgcagattta
     7981 atcggtaaga aaagcgatga aatcttcttt acctctggtg gaacagaagg ggataactgg
     8041 gttatcaagg gtgtggcctt tgaaaaagct cagtttggca agcacatcat tgtttcagcc
     8101 attgaacatc cagcagtcaa agagtcagcc ctctggttga aaagtcaagg atttgaagtg
     8161 gattttgctc cagttgataa gaaaggcttg gtcgatgttg aggcgttagc aggtttgata
     8221 cggcctgata caatcctcgt ttccatcatg gctgtgaaca atgaaatcgg ctctatccaa
     8281 cctattgagg ctatttcaga attcttggca gacaagccga ctatttcctt ccacgttgat
     8341 gcggttcagg cgcttgccaa aattccgact gaaaagtatc tgacagaacg ggtggattgc
     8401 gcgaccttct cgagtcataa atttcatggt gtccgtggtg ttggctttgt ctatatcaaa
     8461 tctggcaaga agattacacc tcttcttaca ggtggtggcc aggagcgaga ttatcgttcg
     8521 acaactgaaa atgtggcagg gattgcagcg acagccaagg ctctccgttt atctatggaa
     8581 aagctagata tctttaggag caagactggg cagatgaagg cagtgattca ccaagctctt
     8641 ctgaactatc cggatatttt tgtcttttca gatgaggaag actttgcacc tcatattctg
     8701 acttttggaa tcaaaggtgt tcgaggtgaa gtcatcgttc acgcctttga agactatgat
     8761 attttcatct caacaacctc agcttgttca tctaaggcag gaaaaccagc cggtaccttg
     8821 attgccatgg gagtggacaa agataaggcc aagtcagctg tgcgtcttag cctagacttg
     8881 gaaaatgata tgagtcaggt cgagcagttt ttgaccaagt taaaattgat ttacaatcaa
     8941 actagaaaag taagatagga gcattcatgc agtattcaga aattatgatt cgctacggag
     9001 agttgtcaac caagggtaaa aaccgtatgc gtttcatcaa taaacttcgt aataatattt
     9061 cggacgtttt gtctatctat acccaagtta aggtaacagc agatcgcgac cgtgcccacg
     9121 cttacctcaa tggagctgat tacacagcag ttgcagaatc tctcaaacaa gtttttggaa
     9181 ttcaaaactt ttctcctgtt tataaggttg aaaaatctgt agaagttttg aagtcttctg
     9241 tccaagagat tatgcgggac atctacaagg aaggtatgac ctttaagatt tctagcaagc
     9301 gtagcgacca caactttgaa cttgatagtc gtgaactcaa ccaaacactt ggaggggctg
     9361 tattcgaagc cattccaaat gtgcaagttc aaatgaaaag tcctgacatc aatcttcagg
     9421 tggagattcg tgaagaagca gcctatcttt cttatgaaac cattcgtggg gctggtggtt
     9481 tgccagttgg aacttcaggt aaagggatgc tcatgttgtc aggagggatt gactcacctg
     9541 tagcaggtta tcttgctctt aagcgtgggg tggatatcga ggcagttcac tttgctagtc
     9601 caccatatac tagtcctggt gccctcaaga aagcgcagga cttgacccgt aaattgacca
     9661 agtttggcgg aaatatccag tttatcgagg tgcctttcac agagattcaa gaggaaatca
     9721 aagccaaagc gccagaagct tatttgatga ctctaactcg tcgctttatg atgcggatta
     9781 ctgaccgtat tcgtgaggta cgaaatggtt tggttatcat caatggggaa agtctaggtc
     9841 aagtagccag ccaaaccctt gaaagtatga aggctatcaa tgctgttacc aacactccca
     9901 tcattcgtcc tgtggttacc atggacaagt tggaaattat tgacatcgcc caggaaatcg
     9961 atacctttga catttcaatc caaccgtttg aagactgttg tactattttt gcaccagatc
    10021 gtccaaaaac aaatcctaaa attaagaatg cggagcagta cgaagcgcgt atggatgttg
    10081 aaggcttggt tgagcgagca gtggctggaa tcatgattac tgaagtcaca cctcaagccg
    10141 aaaaagatga agttgatgac ttgattgaca atctgctcta attcagaaaa tccaaaagaa
    10201 tagcgaaaat cagtaaaaaa gttagttttt tctctaaaaa caggtaaaaa actaactttt
    10261 ttctattttt atgatataat aatataaaat tttgaatata gagagttttc tgacaatgaa
    10321 tcaatcctac ttttatctaa aaatgaaaga acacaaactc aaggttcctt atacaggtaa
    10381 ggagcgccgt gtacgtattc ttcttcctaa agattatgag aaagatacag accgttccta
    10441 tcctgttgta tactttcatg acgggcaaaa tgttttttat agcaaagagt ctttcattgg
    10501 acattcatgg aagattatcc cagctatcaa acgaaatccg gatatcagtc gcatgattgt
    10561 cgttgctatt gacaatgatg gtatggggcg gatgaatgag tatacggctt ggaagttcca
    10621 agaatctcct atcccagggc agcagtttgg tggtaagggt gtggagtatg ctgagtttgt
    10681 catggaggtg gtcaagccgt ttatcgatga gacctatcgt acaaaagcag actgccagca
    10741 tacggctatg attggttcct cactaggagg caatattacc cagtttatcg gtttggaata
    10801 ccaagaccaa attggttgct tgggcgtttt ttcatctgca aactggctcc accaagaagc
    10861 ctttaaccgc tatatcgagt gccagaaact atcgcctgac cagcgcatct tcatctatgt
    10921 aggaacagaa gaagcagatg atacagacaa gaccttgatg gatggcaata tcaaacaagc
    10981 ctatatcgac tcgtcgcttt gctattacca tgatttgata gcagggggag tacatctgga
    11041 taatcttgtg ctaaaagttc agtctggtgc catccatagt gaaatccctt ggtcagaaaa
    11101 tctaccagat tgtctgagat tttttgcaga aaaatggtaa gttaagaaag gaaaaaacga
    11161 aatgcatatt gaacatctta gccactggag tggtcatctt aaccgtgaaa tgtaccttaa
    11221 ccgttatgga catggtggga ttccagttgt ggtctttgct tcatcaggtg gtagtcacaa
    11281 cgaatactat gattttggca tgattgatgc ctgtgcttcc tttatcgagg aaggccttgt
    11341 ccagttcttt accctatcta gtttggatag tgagagctgg ttggttactt ggaaaaatgc
    11401 tcatgaccaa gcggaaatgc accgtgccta cgaacgttat gtgattgagg aggccattcc
    11461 ttttatcaag cacaagacag gttggtttga tggcatgatg acgacaggtt gctctatggg
    11521 agcctatcat gcactcaatt tcttcctcca gcatccagat gtctttacca aagtgattgc
    11581 tctcagtggt gtttacgacg cacgtttctt tgtcggtgat tactacaacg atgatgctat
    11641 ttaccaaaac tcgccagtag attatatttg gaaccaaaac gacggctggt ttattgaccg
    11701 ttaccgtcag gcagagattg tgttgtgtac ggggcttgga gcctgggaac aagatggttt
    11761 gccatccttt tacaagatca aagaagcctt tgaccagaaa caaattccag cctggtttgc
    11821 tgaatgggga catgatgtcg cccacgactg ggaatggtgg cgtaaacaaa tgccttattt
    11881 cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta tttctcccta
    11941 ctatccacaa aactttcaac agtttactat cgaactagct aataaaggca tcacagtctt
    12001 gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata gcttgaccga
    12061 gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag ttgcttttct
    12121 cttttataaa catggtccaa ttgaccgcat cgagtctcac aatgaatact ggcttgagct
    12181 agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg atctcaaaaa
    12241 gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc ctgtggtacc
    12301 tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa tcggtcttcc
    12361 aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac ttgagacaga
    12421 agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt tctttgaaaa
    12481 atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg atggaaagat
    12541 tgtcttctca acaacctttg actacgccta tacaccgctt gacctcatga tttataagat
    12601 ggacaattcc tactatgtgc tcaaggatat ggttcccaaa ctacgcaagt atggggaagc
    12661 gattgttaag gaatttggta tgaaagaacg tttcttccat atcgagtttt tccgtgaagg
    12721 ggatgattat atcgccattg agtacaataa ccgccctgca ggtggtttca ccattgatgt
    12781 ttataacttt gctcattcct tggaccttta tcgtggctat gcagccattg tcgcaggaga
    12841 agagttccca gcgtcagagt ttgaacctca gtattgtttg gctacatcac gtcgtgcaaa
    12901 tgctcactat gtctattctg aggagaactt acttgccaaa tatagccagc agttcaaggt
    12961 taaaaaaatc atgccagagg cttttgcgga acttcaagga gattaccttt atatgctgac
    13021 aactccgagt cgacaagaaa tggatcagat gattgctgat ttcggacaac gtcaagaata
    13081 agaactatcg gattaaggaa attaactccc ttaatccttt tgttttgtct gataaaaaat
    13141 aagagcatcc caacaaggta gctatcataa aacttgttcg ataactattt gaagcaggat
    13201 taggtggcta gaaattgaat tttaacattt caattgagtc atagtattgt gtttgcgtat
    13261 ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt caagatacca
    13321 aacaagtcta ttaatactca atgaaaatca aagagcaaac taggaagcta gccgcaggtt
    13381 tctcaaaaca ctgttttgag gttgtggata gaactgacag agtcagtatc atatacctac
    13441 ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat attcaggtga
    13501 cgcatagata tagttaattg aagctttgtt tgaaatctga taaaataatg atattactaa
    13561 gttttaaaaa ctaaagaaaa gggaagatat gattacaggc gaattaaaaa ataaaatcga
    13621 tcagctgtgg gaaattcttt ggacagaagg aaacgcaaat cctttaacaa atattgaaca
    13681 gttgacttat ctcttattta tgaaagattt ggatagtgtc gagcttggac gtgaaagtga
    13741 tgctgaattt ctagggattc cttatgaggg agtttttcca aaagataaac ctgaataccg
    13801 ttggtcaact tttaaaaata taggagatgc tcaggaagtt tatcgtttaa tgactcagga
    13861 gatttttccg tttattaaaa atctcaaggg ggatacagat gatacagcct tttcacgata
    13921 tatgcgagaa gctatttttc aaataaataa acctgctacg cttcaaaagg caatttctat
    13981 cttagatgtt tttccaacta ggggattaga tgtagatttt gataatgaca aacaaagtat
    14041 tactgatatc ggagatatct atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa
    14101 tggacagttc cgtacacctc gtcacatcat cgatatgatg gttgagttga tgcaaccgac
    14161 tatcaaagat atcatctcag atcccgctat gggttctgct ggcttcttag tatctgctag
    14221 ccgttactta aagcgtaaga aagatgaatg ggaaaccaat acagataata tcaatcattt
    14281 tcataatcag atgtttcatg gaaatgatac ggatacgact atgttgagac ttggggcgat
    14341 gaacatgatg ctacatggag tagaaaatcc acaaatcagt taccttgact cgctgtctca
    14401 agataatgaa gaagccgata aatatacttt ggttttagca aatcctcctt ttaagggctc
    14461 acttgactac aattcaacct ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga
    14521 attactcttt ctttctcttt tcttgcgaac tttaaaacca ggtggacgag cagcagttat
    14581 cgtacctgat ggtgtccttt ttggttcgtc taaagctcat aaaggaattc gtcaggaaat
    14641 tgtagagaat cataagcttg atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta
    14701 tgctggagtt tcaactgcca ttctcatctt tacaaaaact ggtaatggtg gtactgacaa
    14761 agtctggttt tacgatatga aagcggatgg tttaagtttg gatgataagc gacaaccgat
    14821 tagcgacaat gatattccag atattatcga acgctttcat catcttgaaa aagaagcaga
    14881 acgtcagaga acggatcaat ctttctttgt tccagttgct gagataaagg aaaatgatta
    14941 tgatttgtct atcaataaat ataaagagat tgagtatgaa aaagttgagt atgaaccaac
    15001 agaagtcata ttaaagaaaa tcaatgattt agaaaaagaa attcaagctg gcttggctga
    15061 attggaaaaa ttactcaagt agggaggtgg ctgtatgaaa aaagtgaagt tgggggaagt
    15121 cttatctcta aaaaaaggca agaaagccac tgtacttgct gaacaaacaa ctctaagcca
    15181 acgttatatt caaatagatg atttaagaaa taataataat ttaaaattca ctgaaagttt
    15241 aaatatgact gaagcactcc cagatgatat tctgatagca tgggatggag ctaatgcagg
    15301 aacagttggt tatggattat cgggagctgt tggtagtaca attacggtct taaaaaagaa
    15361 tgagcgatac aaagaaaaaa ttatatcaga ttacttggga gtctttttgg aaagtaaatc
    15421 gcagtattta cgagatcatt caacaggtgc aacaattcct catttaaaca agaatatatt
    15481 acttgattta caattagaat tgctaggtat cgaagaacaa gagaacatta tctgtattct
    15541 taatacgatt aaaaggctta ttactaaaag aaaattgcag ttagatgaac taaacttgct
    15601 cgtcaaatcc cgatttaacg agatgtttgg ggaaaataaa atatttgaaa gcattgataa
    15661 cttatttgat attatagatg gtgatagggg caaaaattat cctaaatcag atgagttgtt
    15721 tagtgaggag tactgtttat ttttaaatac aaagaatgtt actaaaaacg gattttcatt
    15781 cgatacaaag caatttatca ctaaaacaaa ggataaatta cttcgaaaag gcaaacttga
    15841 gcgttatgat atagtcttga caacaagagg tactgttgga aatgtagcgt actacgatga
    15901 attaataaaa tataaatatt tacgtataaa ttcaggtatg gtaatattac gtcccaagac
    15961 accaaatcta aatcagaaat ttattatcca tgttttgaaa tttattatcc atgttttaag
    16021 gaataataat tatagtcgag tgatatcagg aagtgctcag cctcagttac caattacaaa
    16081 attaaaaaaa atacttctcc ctctcccccc actagccctc caaaatgagt tcgcagactt
    16141 tgtagccctg gtcgacaaat cacaatttgc ttgtgagata gctataaaag tgtggagaaa
    16201 tagcttgaaa tttagtataa tatagctaaa ctatttgttt aaagtgagaa aaaaatggga
    16261 aattttagct ttcttttaaa aaatgacgaa tatgaatctt tttcaaaacc ttgcattgaa
    16321 gctgagaata tgattgctac atcaactgtg gctactgcct ttatggcgcg tcgtgcttta
    16381 gagcaggctg tccattggat atatagtcac gattcatatt tagaagctcc ctatcgtgct
    16441 actctatctt ctttagtatg ggatgatgat tttagggata tcgtagattc tgaactccac
    16501 aagcagatag ttctgttgat tcggtgggga aaccatgctg ctcatggtgg tgaaattaag
    16561 gaacgagaag cgattttagc tttgcatcat ttgtatcagt ttgttaattt tatcgattat
    16621 tgttacagca atgagtttgt ggagcgttat tttgatgaga agtgcttacc actttcagca
    16681 aacatcaaat accgagaaac tccacaatct atgataaagt tacaagacag tttaccagaa
    16741 ctgcctgatt ttcatgaaca gatggctgct cagtccgtag aagttcaaga gacttatact
    16801 gaaaaacgtg agactgcagc gcaacggcaa gatgtgcctt tccatattga acaattatct
    16861 gaggcagaga caagaaagct ctttattgat atcgatctcc gtttagcagg atggatattt
    16921 gaagaaaact gtcgtgttga gatagccgtt gatggtctca agcacggttc aggaattggt
    16981 tactgtgact atgtacttta tggtaaaaat gggaaaattt tagcgattgt ggaggctaaa
    17041 aaagcctctg tcaatccaga agtaggggaa gtacaggtca aagaatatgc tgaagctttg
    17101 gagaaacata tcggctatca gccaatttgc tttattacaa atgggttgaa gcactatata
    17161 cttgatggtc cgaaccgccg ccagattgca ggcttttact ctcaagaaga attgcaatta
    17221 gtgatggata gacgtcatct tcaaaaaccg cttgaggata tttctagtaa aattagggac
    17281 gatatttccg ggcgtcacta ccaaaaacat gccattgcaa gcgtttgtga agctttctct
    17341 aatcatcgta gacaggcact tttggttatg gcaactgggg cggggaaaac tcgtacagca
    17401 gtttctctag ttgatatctt atcacgtcat aactgggtaa aaaacgttct cttcttagcc
    17461 gatagaactt ccttggttaa gcaagcctat gattcgttta gaaaattact cccagatctt
    17521 tccgtttgta acttcttaga agataaagaa ggagctcaat caagtcgtat ggtcttttca
    17581 acttatccga ccatgattgg agcgattagt ggtcaagaag aagtaaatca acgccctttt
    17641 actgttgggc attttgacct tatcataatt gacgaatctc accgttctat ttatcagaaa
    17701 tacaagtcca tttttgatta ttttgatgca agaattgtag gcttaacagc tactccgcgt
    17761 caagatttag ataaaaacac ctatggattc tttaatttgg agaatggggt tccaacatat
    17821 gcatatgatt tgaaagaggc tgttaaagac ggatatttag tagcctatca ttctatcgaa
    17881 atcaaactga aactacctac ggatggtcta cattatgatg atttgtccga agaagaaaag
    17941 gaacattttg atagcaaatt tgaagacgat agctgtgaaa aagatattga tgggagtgta
    18001 tttaattcct ttattttcaa taaaagtaca gtagaaattg ttttaaatga actcatgaca
    18061 agaggaattc agacagcctc gggtgatgaa attggtaaaa ctattatttt tgctaaaaat
    18121 catgatcatg cggaatatat cagaggtatt tttaacaacc gctatcctga aaaagggagc
    18181 gactatgctc aggtgattga ttatagtatt aagcattatc agaccttgat tgatgatttt
    18241 aaaattaagg agaagtatcc tcaaattgcg atttctgtcg atatgttaga tacaggtatt
    18301 gatgtaccag aggttgttaa tttagtcttc ttcaagaaag tacgctctaa aactaagttt
    18361 tggcagatga ttggtcgagg aacccgtcta tgtaaagatt tatttggacc tgagcaggat
    18421 aaggaaaact tcttggtatt tgattatggg gataattttg attattttcg tgcagatcca
    18481 agagatggag agggtcgtca cattgtttcg ttgactcagc gtttatttaa tatcaaagtg
    18541 gacttgattc gagaacttca gggactccaa taccaagaag atcagtttgc gagagcatac
    18601 cgtcagcagc ttgtctcgga actttaaggt cgtatagaga gcttaaatga gttggacttc
    18661 agggttcgta tggttttaga tacagtttat agctatagga aattggaaag ttggcagaat
    18721 ctaactgctg ttacaagtga aaccattcaa aaaaatctct ctccgctttt atttgatgaa
    18781 gataaagaag atgagatggc gaggagattt gatttgtggt tgcttcatat tcagttgggg
    18841 caactgacag ctaaatcttc cactgttcat atttcccaag tgatgaagac ggctagagct
    18901 ctttctgcta ttggcaatat cccgcaggtt tttgagcagg ctgaaattat caggaaagta
    18961 caggagcctg aattttggaa agaagttaac ttgtctgatt tggaaaaaat tcgtcttgct
    19021 attcgagatt tattacagtt tttggataaa acagaccgta aaccctacta tgttaacttt
    19081 gaagatcgta tactctccac tgttcacgag accacagcat ttttgcaggt caacgatctt
    19141 cggtcttaca atgaaaaagt tgagcattat ttgaaaactc atctggatga ggagtccatt
    19201 tctaagctat accataataa aaagttgaca tctgatgata tgcttgcact tgaaaaattg
    19261 ctttgggaaa aattaggtag taaagcagac taccaaagtc attatgaaaa taaggcaatt
    19321 ccgagattgg ttcgtgagat tattggctta gatagagagt ctgccaatcg tattttttct
    19381 aaatttttgt cggatgagaa tcttaatgcc aggcagattt catttgtaaa attgattgta
    19441 gactacattg tagaaaatgg ttttttagag acgaaagtgt taacgcaaga gccgtttaaa
    19501 tcttatggtt ctgttcaact actcttccaa caccaactac cagtacttcg taatattgtt
    19561 caaatcattg aacttatcaa taatcgagct ggagaagcgg cttaaattct aaagtgattg
    19621 ccatgttgag actcatttaa aattaaaaag agtagaaatt tatgctatat atgagaagtt
    19681 ttattaggaa gaatgtcatc gttttcctag aatacagtat cagttgttaa gtggttgata
    19741 aatttcaaag tagatacttg taccacgatg tttgttgatc gagttattaa caaaagagct
    19801 actttgattt taaagaaata gaaaacaaaa agccgagcaa gaattcaatt gcaggagaaa
    19861 atgaaataat actcaatgaa aatcaaagag caaactagga aactagctgc aggctgctca
    19921 aaacactgtt ttgaggttgc agataagact gacgaagtca gctcaaaaca tggttttgag
    19981 gttgtagatg aaactgacga agtcagtaac catacctacg gcaaggcgac gttgacgcgg
    20041 attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg catagtatca
    20101 agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct accagttttt
    20161 tttaagcata attgttagtt gtagtcattt attattcttc aaagaaaaat ggtggggcga
    20221 attttttcag ttcttcaaag cacttttgag cagtatctgc atcttcacag atgataagac
    20281 agacatcatt accacaaagg gtagcgatag cgtcagggaa gctcaaagta tcaatgatag
    20341 aaccaaagga ttgagccagt ccaggaaggg tttttagtag gacttggtgt tgaactgggc
    20401 gcatccagac aagggcgtct tccatgtaga gttcgagacg tttttcccat tttgagatgg
    20461 aaccattgtt aagaacataa taagcgctat cttcttcgcg gacttttgat aggttcatat
    20521 ttttgatgtc gcgtgagagg gttgcctggg ttacttgaat gtcgttctta gcaagaaggg
    20581 cttgcaactc agcctgtgta tgaatcttgt tttttgtgat aagagcgcgt ataagttggt
    20641 ggcggtgttc tgatttattc ataataatgt aactcctttt agcaaggtaa ggtaagcatg
    20701 gactgagcga ggtcgacagt caagtggtag tctgtattgt cacggatggt gatttcaaag
    20761 tcagtagtat agaggactaa acggagagtg tctccttctt ttagcttgta aatagttggc
    20821 tgcagttcaa attgaacatc catccattca tctgcagtaa tatcctctac taacagtaaa
    20881 tcatttctat tttgtaaatt aaggtaacct tttgtcacga ctcgttgtgc ctctggtcta
    20941 aatggcaatt cacagagatt ttccaacatg tgatagcgac cgttgtcaat ggttctagca
    21001 cttaaaatag ctggataagg ttgtaggtat ttcttttgcc caaattctag cagttgggca
    21061 gataagagcc ccttgtttgt actggatttg atacgaagat tgagctgagc gcgaccgttt
    21121 aggtggagat ctttagtcac aggaaggtta atagtaatct gattagcttt cccttgatag
    21181 agctctgtat tgaaggtttg gtatgtctta ctatagcgct caaaatcctt atctgggtac
    21241 tggttttgaa tagcttgctc ttcttgacca agtgagaagg ttttacagtt ttcttgccca
    21301 ccgaagttat caagtgataa ccaagtctgt ggagctgtat tgtcctgcca gataacagta
    21361 ggaagttgaa agtctgtttc ctgtcctagt aattttttgg ttaataaggc atttatggac
    21421 tcacggaagt caattgattg ccaattgttc atgtaaacat gggcaccatt atggaaaaag
    21481 agatgcttgt gtatatgagt aggaagagca tggaacatct ggtaaacatg aagtggtttg
    21541 acattccaat cctgagaacc atgagtaaag acaacctctg cctttacttt atgggcattg
    21601 agcagataat tgcggtcatg ccaaaactga ttgtagtccc cagtttttcg gtctagctga
    21661 gctttcactt tttctaagtc agcttggtga gcttcattgc cacggatata gtcgccagct
    21721 aagagattac gagaataggt taactcagca agggagtcaa agtcctcacc tggataacca
    21781 cctgggctag tcaccagacc gttttcacgg tagtagttgt accatgatga aattcctgcc
    21841 tcggcaatga taacttctaa accatcgact cctgtagtcg caagaccatt ggacatggta
    21901 cctagatagg aaagtcctgt tgtagcaact tttccgtttg accaatcagc cttgacttga
    21961 cgctggcgcg tgtgatcagt aaaggcacgg caacggccgt taagccaatc gatgacattt
    22021 ttataagcct cgatttgctg gtagtctcca ttagtcatga aacctgtcga gtctttggta
    22081 ccaacacctg agacatagag attggcaaag cctctcggaa ggaagtagtc gtttagtgta
    22141 tagctagagt tgatgtgagt tagcttttcc tcagcctctg ctataagctc agctttacct
    22201 tggggttgga cgagatttag ttgaggttcc tctagctcaa tcttgtgagg aagcttaacc
    22261 tcaagctcaa cctccatctt gtagagagcc ttgtcactag ccttgtcatt ggttccctga
    22321 tgataagggc tggctgtcat gatggcaggg atttttccat caaaacgagg gcgaataatg
    22381 ctaaccttta ctaggtctga tagccctttt tggtcagtat cgacacgaga ctcaacgtaa
    22441 acgacttcac gaatgacatc ctggttagaa aaagtagcca aactcttgcc gttaaagtag
    22501 tggtagtcat tatcctccgg aataagacca tcactaacaa gttggtcgat aagagtattt
    22561 ccttttttgg tgcgagtatt gagtaactga tagagatttt caatcaagtc accatatata
    22621 atgggaaatc cagtttcttt acgaaaaacg tcactatctt cgaagtcaac caaataagaa
    22681 aagcctaaaa gttgaaaagc aacagtataa aaaatatctg ctgtcagttc atcttctgat
    22741 tgaaaaaatg tcagcaggtc tgttttttta tcagctgcta ggatagaaag tgggtagttg
    22801 gtgtcttgat aagtgaaaaa gaaacgacgt aaaaaggttt caagtgagtc tttgtgattg
    22861 gctgtatttt gtaaatcaaa gccacatttt tttagttcag ataagacatt ttcttttgga
    22921 aaattgatat aactatattg attaaaacgc atagaacctc catatagaat gacagttaag
    22981 gttattatat caaaaaaaaa gcagaaaggg aattgttaac ttcaaaagga aataatccaa
    23041 taaaaatgaa taaagtacta aattcaatat agagaacag
//
LOCUS       DAVKJU010000029        22422 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVKJU010000029 DAVKJU010000000
VERSION     DAVKJU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22422
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.029"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..654)
                     /locus_tag="VW191_001084"
                     /pseudo
     CDS             complement(1..654)
                     /locus_tag="VW191_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            complement(763..1281)
                     /locus_tag="VW191_001085"
     CDS             complement(763..1281)
                     /locus_tag="VW191_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEU9623862.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1508..2377)
                     /gene="rsmI"
                     /locus_tag="VW191_001086"
     CDS             complement(1508..2377)
                     /gene="rsmI"
                     /locus_tag="VW191_001086"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU9623863.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFLEIQARIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2380..2697)
                     /gene="yabA"
                     /locus_tag="VW191_001087"
     CDS             complement(2380..2697)
                     /gene="yabA"
                     /locus_tag="VW191_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEU9623864.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2737..3627)
                     /locus_tag="VW191_001088"
     CDS             complement(2737..3627)
                     /locus_tag="VW191_001088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU9623865.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3624..4262)
                     /locus_tag="VW191_001089"
     CDS             complement(3624..4262)
                     /locus_tag="VW191_001089"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU9623866.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLELHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMDLAK"
     gene            complement(4482..5279)
                     /gene="proC"
                     /locus_tag="VW191_001090"
     CDS             complement(4482..5279)
                     /gene="proC"
                     /locus_tag="VW191_001090"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEU9623867.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5283..6545)
                     /locus_tag="VW191_001091"
     CDS             complement(5283..6545)
                     /locus_tag="VW191_001091"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEU9623868.1"
                     /translation="MVSKQEQFEQVQVVKKSINTASEEVKNQALLTMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHEDKAASFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAIA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6555..7685)
                     /locus_tag="VW191_001092"
     CDS             complement(6555..7685)
                     /locus_tag="VW191_001092"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEU9623869.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7765..9648)
                     /gene="cbpE"
                     /locus_tag="VW191_001093"
                     /gene_synonym="pce"
     CDS             complement(7765..9648)
                     /gene="cbpE"
                     /locus_tag="VW191_001093"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEU9623870.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     ASEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIVGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9651..10538)
                     /locus_tag="VW191_001094"
     CDS             complement(9651..10538)
                     /locus_tag="VW191_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9623871.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10528..10989)
                     /locus_tag="VW191_001095"
     CDS             complement(10528..10989)
                     /locus_tag="VW191_001095"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU9623872.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10986..11894)
                     /locus_tag="VW191_001096"
     CDS             complement(10986..11894)
                     /locus_tag="VW191_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU9623873.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11887..12085
                     /locus_tag="VW191_001097"
                     /pseudo
     CDS             11887..12085
                     /locus_tag="VW191_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(12137..12442)
                     /locus_tag="VW191_001098"
     CDS             complement(12137..12442)
                     /locus_tag="VW191_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9623874.1"
                     /translation="MLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSAIIFGYI
                     HINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLLLFSGS"
     gene            complement(12870..13091)
                     /locus_tag="VW191_001099"
     CDS             complement(12870..13091)
                     /locus_tag="VW191_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEU9623875.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13363..14172
                     /locus_tag="VW191_001100"
     CDS             13363..14172
                     /locus_tag="VW191_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9623876.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14353..15228)
                     /gene="aguB"
                     /locus_tag="VW191_001101"
     CDS             complement(14353..15228)
                     /gene="aguB"
                     /locus_tag="VW191_001101"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU9623877.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15238..16323)
                     /gene="aguA"
                     /locus_tag="VW191_001102"
     CDS             complement(15238..16323)
                     /gene="aguA"
                     /locus_tag="VW191_001102"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEU9623878.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALGRPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16320..17447)
                     /gene="nspC"
                     /locus_tag="VW191_001103"
     CDS             complement(16320..17447)
                     /gene="nspC"
                     /locus_tag="VW191_001103"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEU9623879.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCHILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17447..18706)
                     /locus_tag="VW191_001104"
     CDS             complement(17447..18706)
                     /locus_tag="VW191_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEU9623880.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18703..19563)
                     /gene="speE"
                     /locus_tag="VW191_001105"
     CDS             complement(18703..19563)
                     /gene="speE"
                     /locus_tag="VW191_001105"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEU9623881.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            19819..20535
                     /locus_tag="VW191_001106"
     CDS             19819..20535
                     /locus_tag="VW191_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEU9623882.1"
                     /translation="MLYVGIDVAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALANTGHYAFNLLNFLHEQGYKVYTCNPLLIKEFAKSLSLRKTKTDKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASNSRAFCRALKSSPNHQ
                     IEIRNSLDKTIDFSYSLSFS"
     gene            complement(20501..21976)
                     /locus_tag="VW191_001107"
     CDS             complement(20501..21976)
                     /locus_tag="VW191_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU9623883.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDESVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT         6527 a         5147 c         3990 g         6758 t
ORIGIN      
        1 tgtaattctc aagctgtgag ttcattatga tttgtaaatc cgatatatct tcagcagcta
       61 aaaacacatt ttgaaatgtt ccaagtccgt tttcaccaaa actatattct ctcttactat
      121 acttactctt attattctct atatagttag agtcaaggtt cttaacctct tgaagttcat
      181 tttctttact tctgaggtta aaatatttta cttctgaagt taagtttttt gacttctgta
      241 agtcattttc ttttacctga aatatactca taaagtcttt aacataaatg atgttcggct
      301 taccaagtcc aagccttact ctttcgatca gtcctattcc tttttttaca tcaagctcat
      361 ctttggaggt attatgctac acttaaaatt agtaaaaaat gtttttatgg cagttggctt
      421 tgagatattt cttcttttca taatttcttc gacagtaaaa tagataaata ctctgccttc
      481 cttgtctatc cagttattct taaatgacat tcctgtctgt ttaagaagta tggaataaag
      541 gataattgct tctgctgata agccccgaaa ttcttctcta tcaactaata tctccggtac
      601 ttttaagaag ttaaatcttt ccgcttctct gttataaaaa tattcaaagt ccatgcaaga
      661 tcacctccct tcttaaaaat ttgcaaagaa aaagacgata gtttttcttc tatcgccttt
      721 ggtaaccatt tttatgaaat tttttaaagt taaaaatata atttataata ctatcttctt
      781 ccattttctg attttagttc tgaaagttcc aaaaggtgca accgtattta catgaataaa
      841 cttatacact tcccaagtcg ctgttttagt cgcttcatca gcccactttc tcatatgcgg
      901 ttcaaataat tcttcctcac tcaacgaatc aatcattgca gagatagaat taatattttc
      961 atttaatttt gctttcaact cttgcagaga taaatgagcg taggtatctg tgaaccactg
     1021 atataattca ccaagttgat tccatttaaa tttatccgat ggtgttttta cttgaagccc
     1081 ctttctttca tcttcttccc atttaagaac caagttggtc caaccaacct gataagcaag
     1141 gttttctgct ggagttctgt caacttcatc agctctctta tcttttaaat tttctggaat
     1201 attatcaaat tctaaaatat atttctcaaa tgttttctct atctcagctt ttagttcttc
     1261 tttattttcg tatgttcgca aaaaatcacc ttctttctga tttatcaatc ctttgattta
     1321 aaatgacctt atttttctat ttataccttc taagaacgct ataactgctg ctccaaccat
     1381 cggtattccg tatgggctta ataaaaaact taataccaaa gcttctatac caagtgatac
     1441 atctttatat taccatcact aattaagtta aacagaaaca gacagaatta ttacatcctg
     1501 tctcccttta ttgtttttct tcccagtcgt ggtaggcagc gtagagctga cttttattcc
     1561 actggtaaat cttagcgact tccttgatag cttggttttt cttcacacct tgctggatac
     1621 gagcttggat ttctaagaac aagtcttcct cgtctttttc ttccacaccc tgactggcac
     1681 cctcaacaat gagaagacat tcgcccttga gtggcgtttc agcaatgctt tctaataact
     1741 cagagatagt acctcgttgg tattcttcat agattttggt caattccctg accaagacaa
     1801 cggagcggtc accgtagact tctaacatat tttccaacgt gtctgctaca cgatgaggtg
     1861 attcataaaa aatctgtgtt tcaggataat cttttttcaa gccaaaaaat tgcttctgct
     1921 gacctgattt tctcggtaaa aaaccgtaaa agatatgtgg ctgtggcgct aaaccactgg
     1981 caatcaaggc agaaattcct gcagaggcac ctggaactgt cacaactgca atttcttcct
     2041 caatagctgc cttaactaaa tcatgaccag ggtctgaaat gctaggcaaa ccggcatcag
     2101 agacctgagc aatactttgc cctgctttca agaaaccaat caaatcagga attttttcct
     2161 tggcattgtg ctcatgaaaa ctgatctgct tggtggaaat gtcaaaatgc ttgagcaaaa
     2221 gccctgtatt gcgcgtatcc tcagcagcaa tccagtccac ttctttcaag gtctggatag
     2281 cacgaaaagt catatcatct agattgccaa tcggcgttgc cactagatac agcttgccat
     2341 agggagactg ccccttaaaa cttttttgaa tttgcatgct tactccctgt ataacaactc
     2401 atcacagaac atacattctt cgtcctgctc tcgacgttgt ccataaaaat cattacatac
     2461 gtgaaatcca tcacggtaaa tacgacggac actttcgcga acatgcttgg ccttgacggg
     2521 agcgtctgct tccacctcac ccaagcgttc tcgcaactta ctattttcca agcgaagagc
     2581 tgtattttct tctaccaagc tcttgagatt tttcttgatg gcttccacat cggctaaggt
     2641 taccaataat tgttgggaaa aatcatccag cgcgtcaaat aattcttttt tgtccataaa
     2701 acagcccttt cctttcttta tcattcattt ttgagtttat atttctttca agaccagata
     2761 ttccatggca ttttgaaagc tgacattagc ttgccacatt tttctagctt ctagtaaatc
     2821 ttgtagaatc actcttactc ttacctgcaa gaggtcctgc ccacagagaa cttcaagaat
     2881 ccgtaaaacc tgatcctgtt tttccttatc atctgccaag ttggctaatt tggcaacctg
     2941 tagataactt tcttttttct tagctactaa ccaagtcagc aggcgttcac tttcatcgac
     3001 caaggtccaa aaacttgcct gattagccaa cttttctgct tcagctcgcg attgactaaa
     3061 cttagctaaa agagtcgctt ttttcttaac aagtcccatt tgttctaaga gtaagataag
     3121 tttttcttct tgctttttaa agtggaagat ctgagtccga cttcggattg tcggtaacat
     3181 cttttcctca tcgctagtca agaagaaaat ataaacttca ctctggggtt cttcgatgac
     3241 cttgagcaga gaattggctg cgttgggatg cattttatcc gcttgctcga taataaagac
     3301 ctgttgctgg ctttcaatcc ctgcttgaga aaactgtccc accaattccc gaatgcgttc
     3361 tgtcttgatg acctgattga ctggcttaat caaggtgaca tctggaaact cttcctgttc
     3421 aatcagcttg caacttcggc atttctcaca tggtaagacg ccaactttat ccgtacaaaa
     3481 gaggctctta gctaaaaatt gcgccatttc caagcttcca aagaaacctg aaaagagata
     3541 ggcgtgattg agctggtctt gttctaagat acggacaaaa cggtcaaact gagctggttg
     3601 ccaagccttt agttgatctt gtttcatttg gccaagtcca ttccgtcaaa caagacagcc
     3661 ttggtagttt ccacaacttg ctccaaaggg agactagcat caatcttgac aatgcgattt
     3721 ccttcttttt ccaggagaga aaggtagcct tgacggactt tcttgtgcaa ttccaaacct
     3781 tccaagtcca gacgattgac ctcgcggtca ctattcgcag caatgcgagc cagcccttct
     3841 tccacctcga tgtcaaaata gagtgtcaaa tcgggtttga ggccatctgt cgcaaactga
     3901 ttgagccagt caatggcttc aatatctaag ccacgaccaa atccctgata ggcaacagaa
     3961 ctatcgataa aacgatccat gatgaccaac ttgccagctt caagggctgg aagaactttt
     4021 tccaccaaat gctgtctgcg actggcaata tagagaagta gctctgtttt agcatccatc
     4081 tgagtatgac ttggatccaa aatcacttcc cgaatcttct ccccaatcaa gactccgcca
     4141 ggttcacggg tcgtcagcac ctttactcct ttttcctcta aaattggtag cagagcctct
     4201 aaaacactgg tcttgcctgc tccctctggt ccctcaagag agactaaaaa tccttttgac
     4261 atgtctaact catttctttt ttactacttc tattctatca aaaaaatagg ggtttgtgac
     4321 aatctttttg tcttctcatt tcatactcaa tgaaaatcaa agagcaaact aggaagctag
     4381 ccgcaggctg tacttgagta cggcaaggtg aagctgacgc agtttgaatt tgattttcga
     4441 agagtatcaa ccaccataaa agaggcagat aaaactacct cttatttacc tagttcttgt
     4501 gttcgtttat aggcttgatg aactgcctcc atgaccgttc ctcgaaaagc atgcgcttct
     4561 aggcttgcta cgccagcaat agtcgaaccg cctggactac aaacttgatc tttcaaaact
     4621 ccaggatgtt gctggctttc taggaccaat tgcccagctc ctaccacagt ttgggctgcc
     4681 attttcaagg ctgtttctcg tggtaatccc gtctgaacac ctgcatctgc caaggcctca
     4741 ataaagagat agacaaaggc tggtccacaa cctgcaagac ctgtcgctgc attgattaag
     4801 ctttctccta attcaaccaa gagaccagcc ttggctaaaa gctgacaaaa gatctcactg
     4861 tcctcagccc tgcaattagg agacaaggca taactaatca ctccttgccc gatagaagca
     4921 ggggtattag gcatcatacg aataattcgg tgttggcttg ggataagact tgctagtttt
     4981 tctaaggtca atccagctgc catcgaaatc aaaagaagac tttctctttt ttcaaggatg
     5041 gtctggtatt gagaaagcag ttcagaaaac tgagcaggct taacacctag aaaaatcaca
     5101 tctgcttctg cgaatatttc ttcattgtta gaagtctgac caccgaagtt agcgatgaaa
     5161 gcatccacct tgacttgact acgattggca aggagaatct catctgacgt cctagtctgc
     5221 aagacagatt ttgccaagct agcacccata ttccccaaac cgataaatcc aatcttcatc
     5281 tcttactccc ttatctgtcc atcaccagtg accacatact tgtagctagt caattccttc
     5341 aagcccatgg gaccacgcgc gtgcaatttc tgagtagaaa tccccatttc acaaccaaga
     5401 ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa catacaccgc tgcagagtcc
     5461 acttgatctg taaagtatgc tgcagcttca gcattttccg tcacaatggc atccgaatga
     5521 tgggtactgt gggattcaat atgcgcaatc gcttcttcta aactgctcac aaccttaaca
     5581 gctagaatat agtctaaaaa ctcggtatca aagtcttgtg cttgagcagc ttgacctgaa
     5641 acaaactggc ttgctttgct atctaggcgg aattgaattg gttccaaccc agcttctttt
     5701 cgatctgcaa ccaacacttg ctccaagcga ggaaggaagc ttgctgcctt gtcttcatga
     5761 accagcagaa cctccatggc attacaaaca gaaggacgac tggttttagc attgttgatg
     5821 atagacagcg ccttgtcttc gtctgcatcc ttatccacat agacatggac aatcccagtc
     5881 cctgtctcga taacaggtac aatcgcattc tcaaccactg cattgatcaa gccagctcct
     5941 ccacgaggaa tgagaaggtc tagatagccc ttagccttca tcatagcata actactttca
     6001 cggctagtat cctccaccag ttgaatcaca tttggatgaa tagtagtcgt ctccaagccc
     6061 ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg catccttacc actacgaaga
     6121 acaaccgcat ttccactctt aagagtcaaa gcagccgcat cagacgtcac atttggacgg
     6181 ctttcataga taataccgat gacacccata gctacacgtt tttttgtgat aaccaaacca
     6241 ttttcaagct gacttgtttc taaaacttca cctattggat ctggtaaggc aaccacttca
     6301 cgaattcctc ttgccatcgc ttctatacga tctgcatcca aataaagacg atccaacatc
     6361 acatctgaga ttttcccctt agccgctgcc atatcgaggg cattagccgc taaaatttcc
     6421 tcagtagcag ccactaagtg atcagccatg gttagcaagg cttggttttt cacttcttca
     6481 ctagctgtgt taatcgattt tttaacaacc tgtacctgtt caaattgttc ttgtttactc
     6541 accatagttt acctctaaaa ttctgtaaaa agtagttgga tttcaggagt aatggaaatc
     6601 cagtcgtcac ggtaaatcaa gacacccttg gctttttgag aacgcaacat atcctccaaa
     6661 gcagatgctc cgaattgcac gcgtcctttt ccaagtgatt ttccactttc cttatcaaat
     6721 actgtcacga tatcaccgta agaaaaggct ccttctgctt caacgatacc agataagaga
     6781 agactctttc catgttgaga gagagcttcc gcagcccctt tatcaaccca aatagaacct
     6841 tgactctgag catagaaggc aagccattgt ttctgggtac gaagcccctt ctcttgagca
     6901 acaaagtaag aaccatcctc ggtctcctct gccgcctcaa tcatggaatc tgatttcaag
     6961 gatgagcaga tataaacagg aactcctgat tccgtcgcga tagttgcagc cttgattttg
     7021 gttaacatac ccccagttcc gtttgacgaa ccagctccac cagccatatc aataatctca
     7081 cgattgatgg tctcgattct ctccaagcgt ttggctcttg gatctgaatt aggatttcca
     7141 gtatagagac cgtccacatc tgtcaaaaaa actaaaaggt ctgcttggac catcgccgct
     7201 acttgagcac ttagagtgtc attgtcccca accttgagct catcaataac gacactatca
     7261 ttctcattga tgataggaat tgccccacgg ttgagcaaaa ccgacaaagc ctgatgggca
     7321 tttttataac gacgcttatc cacaaagtca tcttgggtca gcaagatttg tgcagaaacg
     7381 atttgacgca agagaagatt ggttgtatat tcttccaaca aaagcccctg ccctaccgct
     7441 gctgaagcct gtttatcagc aattttagtc ggacgctttt taaatcctaa ggctccaaaa
     7501 ccagccgcaa tggcacctga agacaccaaa atcaactcat gaccagcctc gtgcagcata
     7561 gccaactgct gggtaatatc ctttacctta ctacgtgata aacttccatc ctcatttgtc
     7621 agagaagaag tacccacctt aaagacaatc cgtttgtatt tcatagtctc actccgatta
     7681 ttcatattta tccattataa catgaataac aggaaataga aaagagttga taggaaaaag
     7741 aaggccctaa aaccttcttt tctactactg ttctgattcc gatttgttta cactcgtttc
     7801 agagctttgt gaagttgagg atgtcaaatc ttgactagtt gaagaatttg taacactttc
     7861 atgttgactc gtttctttat tttcaagatc ctttttcact acttctttgg attcttttat
     7921 ttctgaatga cttgtagttt taattgtagc agatttctcg atcgaaacat ccactaacca
     7981 ggcaccactt gaatccacag tatagccttc tggtgtctta ccattcacaa gtaattgacc
     8041 attttctttc aagaagtacc atttatcctt gtccttgatc caacccacag ctcgtgaacc
     8101 gtctgtcttg tagaaatacc aatcatttgc ctttttatgc cagccttgct tcatagctcc
     8161 tgaatcttcc agataataat gttgaccagc aacttctatc tcacctgtct tcatgacacc
     8221 agtagaatcc atatagtacc aggtttcttt atcttttacc cagcccattt tcatttcccc
     8281 tgatggagca aagtaatacc attgccccat atattgaagc catcctgtct tcatagaacc
     8341 atcaacatcc aaataatacc actgctcttt atcttgaatc caaccaattt ccatctggtt
     8401 ttctttgtta aaataatacc agattccaac aattttcttc caatttttag cagcaacacc
     8461 agagtctgtc aaatagaacc aacgattgtt ccattttttc cattgattct gtaacaagat
     8521 acccgtttgg ttaaagtaat accattcacc cttaatttca ttccaaccga cagcatattc
     8581 tcctgtagaa tcaggcgctt gataccacca gtttccatat gaactcttat gccaaccagc
     8641 ttgaaaactt ggaataggct tgtaggatgt tgaaatattg acaaaaccgt cttttcgaat
     8701 atcaaaaact gtcgcatcat agtctttgct ggctgcgttg attctctcaa ttcctcgttc
     8761 tttgagccaa ttaacatact cactatcaac accatttttc caaggtagac tatccgaagt
     8821 ttgaacaatc aaactcggac tcaaattttt aatgaaatcc ttggtatttg atttattggt
     8881 atcatggtga tgattaaact tcatcaaatc aacttttcca atgagaggac catacttgtc
     8941 ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa attttcttgc cattgacttt
     9001 caccacgcta atcaaggaat tggaattgtc atcccaaatt ttctttaatt cacccgatga
     9061 atcagtttca ttttcataat tatagagctg aatatccatg tccccaaact gaaaatgagc
     9121 atccccttgt gtgatgtttt gaataactga aacacctttt tctgaagcag tctgtaaaac
     9181 cttatcataa ccgtacagat tatcccatag acgttcagaa ttagtaatac gattatcact
     9241 atatttttta agatagactc ggtcaactgg ataggtagac agtaattcat caacatttcc
     9301 aatatgatca ctgtgggtat gggtcaccaa aataaaatca agtttttgga cacccaattc
     9361 cttcaaacga cgaaagacac ggtctgttag aacatgctta taagacgttt caattccttc
     9421 tctccatgga tagcgagaat cacttccatc tgggaaatca taatcttctc ctgtatccac
     9481 catggcaaaa tgtccattgc tttcaagaat aatcgcatca ctgccacctt cttgaacatt
     9541 gataaagtgg attttatttc ctgaactttc ttgagcctga acattcccat accatgacaa
     9601 acctaaaaaa gcgcctacaa gtgctaaact agttaatttc tttttcattc ttactttctc
     9661 aatctctcca aggtttcctt aaaaatctct gggatatctg ctttaaattc caaggtctta
     9721 cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa gaaattgtcc atgtcctttc
     9781 aaagtcttgc gaggaccata gacctcatca ccagcgactg gatggccgat ataagccatg
     9841 tggacacgga tttgatgagt acgccctgtc tccagttgca actctactaa gctataatcg
     9901 ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct tccctttagc agttacagcc
     9961 tgtttcttac ggtctttttc actccggcca atcggcgctt caattacacc acgatcatta
    10021 ggcagatttc catgaacaat cgcccaatat ttgcggagag actttttatc tttgagttct
    10081 tgggcaagta ctagatgcgc atcatcgttt ttagcaatca tgagaagacc tgacgtatcc
    10141 ttatcaatac ggtgaacaat ccctggacgc agaaccccat tgatacccga caagtcctta
    10201 atatgataca tgagggcatt tactagggtt ccactggtat gaccagcact cgggtgcaca
    10261 accattccct gaggtttgtt aacgacagcc acatcctcat cttggtagac tatttctagc
    10321 ggaagatcct cagccacata ctctaatacc tctggttctg gcacatggta agtgacgaca
    10381 tcaccctctt ggactgtgta tttagctttc ttgacttgac cattgaccaa gacctggcct
    10441 gatttaattt gttcattcgc gagactacgt gataattctg acaaatctga caaagcctta
    10501 tccaaacgca gaccaccagt ttcaatttta atttccattt atttcctctt ttagcattgc
    10561 aatcaataaa ataatcactc caaccgtcag atagctatct gccacattga aaattgcaaa
    10621 gttgataaag tcaaggtgga acatatccac aacaaagccc tgactgaccc tgtcaataaa
    10681 gtttccaaga ccacccgcga ttattagagt caaacccaag accatccaga atgagtcctc
    10741 catgtgttta tgtaaatacc aaatggcacc tatcacgaca accagagtaa tgacagcgaa
    10801 taacagctgc tgatcttgta agatagaaaa ggctgcacct cgattttgca ggtaggtcaa
    10861 gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt ggaatctgct ggacgatata
    10921 ggatttgacc aactgatcca gcccaattaa aagcagtaca atgactgcca ctattgctct
    10981 ttttttcatg atttcctctt ttgatcaaaa tattcttgca tgacttctac gaagagagtc
    11041 ccagcttgac taagctccac ttcttcacgt ttaacataga ccatgcggtt atctaggtta
    11101 tccttgagac gaataactgt aatgccatta acactgtcac tatctaaaaa tccagaacct
    11161 gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg tggcacggtc tgtcacatta
    11221 aacatctgtg agctagcgct ggtatcgaca aagttctctg aataataaag gtactcgtct
    11281 ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat cctccatgac taattcctct
    11341 ttctgggcta aaggatgacc ctcacggaga taaatatggg tatggaaagg aatcaattcg
    11401 atgacctcca gacctaattt ttcaacccgt tgcataatcc cctttttatt ttgattgttg
    11461 aggtagataa tcccaatctc actatgccct tgcgccactt catctaatat ttgaacagta
    11521 gttgattcaa aaatacggaa gttcttatag tcaggatagc gctctgaaaa ggccgtaata
    11581 gttggtggca agaagtcata gtgctggcta gcaacggaaa attcatcttt ttcttcttca
    11641 ggattggcat actgattttg aaaaatatca aatcctttaa ccaattcttg cgctttttca
    11701 taaaattcca tcccacgacg ggtcaagaaa gtccctgagc tggtccgacg gaaaatctta
    11761 aagcccaact ctttttccaa atcacgaaca gaaatagaca gactcggctg actaacatac
    11821 atcttttcag cagcttcacg aaaagtacca ctattggcaa tagccacaac atagcgtaat
    11881 tgttgaatgt tcatcttcta ccccccaact tctttatctt ttcattatac catattttag
    11941 aagttttcca aaaaggaaaa aagaacatcc tattcttctt aactatcttc actatctgcc
    12001 tttctcacgc caatcttatt ttcaaaatca attcaaaaaa ataagtggct acacaccact
    12061 tcagtatcgg aaagaaaaac gttgactatt tgtgaaaaaa gaaaatgccg gaaagttccg
    12121 acattatttt agtaagctaa cttcctgaaa ataggagcaa ccacacatcc cagaaggcag
    12181 tgatattaat gagaatatga actagtattg gatagtagag atttttagtc atgcgataca
    12241 atagagctaa aataagacct ccactagcat aaatgaaaaa agcaagagga gttaaagcaa
    12301 aattgatatg aatataaccg aaaataatag cagaaagcaa aacatctccg taccaaggtg
    12361 agtttttgaa aaaggttgtc ataagcacac ctcgataaat caattcctca gcaatagggg
    12421 cgatgaagca aacgatgagc aagaaataag ggaactcctg tctccccatc atttctaccg
    12481 ttctaccgtt tcattcaaag aaatctgatt tgaagacagg gatatgaaat acgaaaagag
    12541 gaagtcagac atatatgaaa tgatgtagcc cagtaaaagg tagatgaagt acctcagctg
    12601 ccacttgaaa tgaaaaattt tctttcctgc aaaaaccagt agatagatga ccgctaagaa
    12661 aagaactccg ctctccatca aaagcagaat ctgaaaaaat tcacgacttg ctggtagata
    12721 gggctttgcg aggtgattga aaactctcca aaaccaagtt gatttgtaaa aaattaagga
    12781 caatgctagt aaaatttgaa tagcccgttt tttcatatta aattctctgc tttctccact
    12841 tgtttcttca tattactaat aaattttact taaaatctcg cagcacttcc tttgcaaaga
    12901 tgattgcctc ctccaatata tcctaaatca taggtgcctc tgggacaaaa tccatgaacc
    12961 ggcagagtta atactggttc tatttcccaa tcgtacctac cattaggatt tttaccatat
    13021 gaatctgctc caccttgtat cagctctaat tcttcatttg ttagatttat attttttgtc
    13081 attaaaatca tgatatattt cctccattat tcttcagatt agttgagtaa tctgatactc
    13141 atacctactt acaaaaaaac tattatatta agttggtttt ttaatttgtc tagttgcaac
    13201 attgacaaac atagtatagc atatctttgc caaatatcct cttcaaatca tgaattgtca
    13261 tcaaaacatc ttaaactata aaatcaatta gtctcaagct ttctatcaat ttcttctcca
    13321 aaatatgcta taataatagc aaaagataaa gaaggaagac ctatgattaa actactagcc
    13381 ttggatatgg acggcaccct cctcaatgaa gccaaggaaa tcccacaagc tcacattact
    13441 gctattcaca aagccattga aaaaggtgtc aaactggttc tctgtacggg tcgccccctt
    13501 ttcggtgtcc tcccctacta caaaaaactg gagcttgacc tccagaacga atatattatt
    13561 gttaacaacg gttgttcaac tcaccagacc agtgattgga gtctagttga ctggaaagaa
    13621 ctgagtccag ctgacatcga atacctctat gaccttgctg aaaagagtga tgttcagttg
    13681 acactttttg acgagtcaca ttattttgtt ctcggtggca aacccaatca agttattgaa
    13741 aatgatgcta aactagtatt ttcagacctg actgaaattt ctcttgaaga agcgactagt
    13801 ggaaagttcc ggatgttcca aggtatgttt ttaggaacaa aagaacaaac agacgatttt
    13861 gagcagcgtt ttgctgaaga gctttgccaa cgattcagtg gagttcgttc gcagcctgtc
    13921 atttatgaag caatgccgct tggaacgaca aaggctactg ctctttcacg actagctgag
    13981 attttgaaga ttgattcctc agagattatg gccatgggcg atgctaataa cgatatcgaa
    14041 atgctccagt ttgcagggct tgggattgca atgggaaatg ccagcgatta tgtcaaatct
    14101 cttgcggatg ccgttacctc aagcaacgaa gaagacggcg ttgcgcgtgc tattgagaaa
    14161 tatattctat aaaaaagaaa agcaaataga cagaagttac tgctatttgc ttttttgcta
    14221 atattttaaa atacggacta atcaatagac aagaatagcg aatcataaaa atcgatttac
    14281 tagatgccat agtatcttat agttgctaat aagaagttag tctagcagaa tgaatctctc
    14341 atttctccca cactaatctg taatttgtct atacatttct ggtcttctat ctcgaaacaa
    14401 gccccagttt aggcgttcac ttgctccctt gtctaggtca taagtagcta acagaacagc
    14461 ttcttcttgt ctttcagctc gttctagaat agctcctgtt tcatccgtca taaaggagga
    14521 accgtagaag tcaagactgg aactctgtcc gccattttcc tcactaggag taacctcctc
    14581 taaaccataa cgattggctg cgatgactgg aacaatattc gctgctgcgt gcccttgcat
    14641 agtacgttgc cagtgaccac aactatctgt atccaaaatt ggctctgaac cgatagctgt
    14701 aggataaaag agcaattcag caccattcaa tgcaagacag cgcgctgttt cagggaacca
    14761 ttgatcccaa cagataccga taccaatctt agcatagcga gtattccaga ccttgaaacc
    14821 agtgttacca ggcgtgaaat agaatttttc ttgataataa tggtcatctg gtatatgggt
    14881 ctttcgataa acgcccagca cttccccatc tgcatcaatg acggcaatag agttatacaa
    14941 gacattacca tctttttcat agaaactgat tggtaaaaca acttgtagtt ccttagcaat
    15001 caccttaaaa tgctgaatgg cagtattttc cgctacagat tgggcatact ggtagtagtc
    15061 atactgacgt tcctgacaga aatagggatg ttcaaacaac tcgggcaaga gaataatttg
    15121 ggctccttgc tcagcagcct gacgtactaa acgctctgcg gtttggatat ttgttgccac
    15181 atccttagcg cattgcatct gaatggttgc aactcttaca tttctcatct ttttctccta
    15241 ttctggaatt tgttgggtga tacagtggat attgccacca cctaagagaa tatctctggc
    15301 tggtattccg acaactttac ggtctgggaa acacttgctg aggatatcta aggccacttg
    15361 gtcgtttaca tcctcaaact gtggaaccaa gacagccttg ttggcgatat aaaagtttac
    15421 gtaggaagct gctagtcgtt cacctgcgta tcgcttttct tctccttctt catagatgta
    15481 gcctggcaaa tcttcttctg tcacaacttg tcgaactgca gggataggca atttatgaat
    15541 ggtgaagtga caaccttttg catctgtttc ctgttctaag agttcgagat ctgcttttga
    15601 catggcatac tggggatcat tttcgtcatc tgtccaagct aaaacaacct cagcaggacc
    15661 aacaaaggca gcaacattat cgacgtgttc attggtttca tcctgataaa taccataagg
    15721 aagccaaata actttttcag caccaagact ttctaataat gtgttttcaa tctcctcttt
    15781 agtcaggtta ggattgcgac caggactaag caagcaactt tcagttacga gaatagttcc
    15841 ttgaccatcg ctatggattg cgcctccttc cagtacaaaa ggtttagcat catagacagg
    15901 ccttcccaag gcctcagcaa aacgactggc tacttggtca tcctcttcat aatcttgata
    15961 aagaccatca taggtgcctc cccaagcatt gaaggcccaa tccacggcta atttcttacc
    16021 tttatcattg acgagaatgg ttgggccagt atcacgcgcc caggcatcat tggtgggaat
    16081 gtctaaataa acaaccttgt ctccaagata ggattgggct tcagatagat aggcctgctc
    16141 caccaaaaga tagactcttt ccccttctgc tatggtctcg atgatctgag taaatgatct
    16201 tttagcagcc tttccttgaa aaggccatga tcctggtcga gtcggccata tcatgagggt
    16261 accatgatgg ggttcgtact ctgctggcat gtgatagcct aatttttttg gactgtccat
    16321 catgataatc tccctttaaa gtcttgatag ccaaaagctt tgagtaagct acagtctccc
    16381 tgttcgtcca tgagatagag acttggcaat ccaataccat taaaggtatt atttttgaca
    16441 aaagaataaa tggccatgtc ttgaaaataa agtctgtctc cgatttggac tggattttca
    16501 aaactataat caccaatcac atcgcccgtc agacaggtat tagaagaaag tctgtaggta
    16561 tgggcttttt cctgtgcctc aaagccattt ctcaaaggtg gacgataggg catctcaagt
    16621 acatcaggca tatggcaggt cgcagaggcg tctaaaacca agatttccat accattttct
    16681 acaatatcta atacctcagt tgctaaataa cccgcattaa gcgcaatggc ttcaccaggc
    16741 tcgatataga tttcaagatt gtaagttttt cggatacgct tgatttctga aatcagcaaa
    16801 tccacatcgt aaccttctct tgtaatatga tgaccaccac ccatattgag ccattttacc
    16861 tcatgtaagt agggaccaaa ctgttcttct actgctttca aagttgtttg taaatcatct
    16921 gctccctgct cgcaaagggt atgaaaatga agtccatcaa ccaaatctag caaatcactc
    16981 ggaatcttgt ctatagtaac tccaaagcga gaacctggtg cacaagggtc atagagcgcg
    17041 tgatctcctt gagttgaaca ctgagggttg aggcgcaaac caacactgac accagcctct
    17101 cgacaacgcg aaccatgctt acgcaactgt ctctctgagt taaagacgat atggtctgtt
    17161 atcccaagca attcctccat gtcctcatcc ttgaaagctg gtgcaaaaac atggacttca
    17221 ccaggaaatt cttcccttgc caatttggcc tcatagagtc cactagctgt cgtacctgat
    17281 agatactggc taatcaaggg ataagttttg tagagggaat atgccttctg ggcaagcaag
    17341 accttgcaac cgacctcttc ttgtacatat tgtagaatgt ggcaattagc ttctaacttg
    17401 gccaagtcaa taacataggc tggtgttggt acttgttcta acttcattag tccaccattt
    17461 gtggattttc aaccacaacc catggcaaac catactcatt caaagcttcc atgaatggat
    17521 ctggatctaa ctcctcaagg ttatacactc cagcttgttt ccaagttccg ttcatgacta
    17581 attttgtccc aatcatggct ggaactcctg tcgtataaga aatagcttgc gaaccaacct
    17641 ctgcgtaaca ttcctgatgg tcgcagacat tgtagatata gatagtcttt ttaacaccgt
    17701 ctttgacacc tgtaaagata catccaatat tggtttttcc gactgtacgt ggcccaagac
    17761 tggcaggatc tggaagcaag gctttcaaaa attgaattgg aacaatttct tggccgttaa
    17821 agttaatggt atccgtacga aggagtccaa cattttcaag acatttcatg tgcgtcaagt
    17881 aagattgacc aaaagtcata aagaaacgaa tgcgtttgac acctggaatg ttcttggcca
    17941 atgattcgat ttcttcatgg tgaaggagat acatatcttt ttgtccaact tgagggaaat
    18001 catactcacg cttgatagac atagcttcga cttcgaccca tttcccatct tcccagtaag
    18061 aacctggagc agaaacctca cggagattaa tttctggatt aaagttggtt gcaaatggat
    18121 aaccgtggtc accgccatta cagtctaaaa tgtcgatata atggatttca tcaaaatagt
    18181 gtttgagggc ataagctgaa aagacactag ttacacctgg gtcaaaacca gaaccaagaa
    18241 gagcagtcaa gcctgcttct ttgaatttct cttgataagc ccactgccat gagtagtcaa
    18301 agtaggctgt aaaaccaagt tccttacaac gtttttcgta gatagcacgc cactcagggt
    18361 cttctgtgtc ttctgcttcg tagttggctg tatcgatata gtgaacacct gttgccaaac
    18421 aagcatccat aatggttaaa tcttgataag gcagagctac attcaaaaca gcttctggtt
    18481 tgtagctttc aatcagggca atcacttctt caaccttgtc agcatcaagt gctgcagttt
    18541 caattttagt acttgttttg ccttctagct tcgctttcaa gtcatcgcat tttgacttgg
    18601 tacggctagc aatcataatc tctgtaaatg tttcgctatc ttgacaaatc tttgaaatag
    18661 caacttgggc aacgccccca caaccaataa ctagtaaacg actcattttt ttccttcctc
    18721 ttcttctaaa atgtcctcaa catacttggg caacataaag gatcccacgt gtaagtttgc
    18781 agtgtagtat tctgtgaaaa gctggcgttt tttccaacct tccttgtcaa aatctttgac
    18841 agggtggtat tttttcgatg caaatccaaa caaccaatag ccagctgggc tagttggaat
    18901 atgggcctga taaacccgac tgattggaaa ggattgattg accttgcggt gcatgcttcg
    18961 gcaggccgac tcatcctcgt caaagaaggg actcccatgc tggtaaatca tgatgccgtc
    19021 ttccttcaga gctcgataac tattgccgta gaattccttg gtaaagagtc cttccgtatg
    19081 gccaaatgga tctgtcgcat cgttgatgat aatatcgtaa tcatcttcgc agtttcgcaa
    19141 aaagcgtagc ccattttggt agtaaatggt aacacgagga tcatctagcc ctgcagcaaa
    19201 gtctgggaaa tactcacgac agacctcgac caacatctca tccggttcca cgatatcgat
    19261 ttgttccagt tcaggataga gtgttaatac ttgggcaaca ccgccgtcac cacccccaat
    19321 aaccaatact ttctttggat ttgggtggac agccatggga acgtgaacgg tcatttcatt
    19381 gtagacgaaa tcatccgcat ctgagaacaa gacatggcca tttaaaatca gtattttccc
    19441 aaaagctggc gtatccaaga cttcgatatc ctgccattca ctttttccag cgtaaagttg
    19501 cttggctgtt ctcagagaca atttgacatc tggagtatga acttcagaaa accataaatc
    19561 catctatttc tccttatatt atatttagcg cagttagcta ctgccaaagc ccaagtggta
    19621 tacttggaat aagccactgt ggattagttt attccctcca atcgcttgac gatttctgaa
    19681 aggctctaac cacagttttt tgtttcaatt ggtaatgagt ttgataacgc atagacgttt
    19741 tatctcgtct aaggtaacaa ggcaaaaaat tcgtggataa ccaagtagac ttgaaatact
    19801 acatctggag gtactcccat gctttatgtg ggcattgatg tcgctaaaaa taaacacgat
    19861 gttacagcct tgaatgttcc aggaaaaact gttcttaaac cactcacttt ttcaaataat
    19921 aaagctggtt ttgaactctt agatctgtct cttcgacaac tcaaccaaga ctgtctcatc
    19981 gctcttgcaa ataccggaca ttatgccttt aatctactga acttcctgca tgaacaagga
    20041 tataaagttt acacctgcaa tcctttgctc atcaaggaat ttgccaaatc attatcactt
    20101 cgcaagacta aaacggataa gaaagacgct catggcatcg ctcttaaact tctttctgac
    20161 cccaatcgtg aacaatttca acacgataat cggcaagtag aactaaaaat actggctaga
    20221 catattcatc gtctcaagaa aaaacagtct gattggaaag tacaatacac tcgttgtctt
    20281 gatatcatct ttcctgagtt ggataaaatc gttgaaaagc attcagaata tacctaccaa
    20341 ctcttgacat gctaccctaa tcctcagaaa aggcttgagg caggatttga taagctgata
    20401 gaaattaagc gattgaccgc ttctaattca agggcttttt gccgtgctct aaaatcatcg
    20461 ccaaatcatc aaattgaaat aagaaatagt ttagataaaa ctattgactt ttcttatagt
    20521 ttgtctttct cttaataacg ttgatatgat tgacctctgg atcttccgtc ccttggaggg
    20581 agcaaccacg ttccttggcg aattggatat agtctacaat ttctcgtgta atgcgttcac
    20641 caggagccaa gataggaatc cctggaggat agcacatgac aaattctcca cagacctgtc
    20701 caacagactc atctaaggtc aaactttttc tctctgaata gaaggcttct tgtggagaca
    20761 gcaccaactc gggctgaata tattctccag ctattaagtc cttcccatct cgtgaataga
    20821 gtctcttaat atcagccaga gcaccaacca agcgctcgat gtcttggatg cggtcgccga
    20881 tggaaatata ggccaagata ttgccgatat caccaaactc gatctgaatg tcgtattcgt
    20941 ctcgcaagag gtcataaacc tcgatacctg ttaagcctat tccctgagtg taaactgaca
    21001 acttggttac atcaaaatca cagaccgaca caccatctat taactctttt gagtaagcat
    21061 agtaaccgcc aatggcattg atttcatgac gagcgtactc ggatagctca atgacttcct
    21121 caaacgactc tttaccacga agggccaagt tgcgacgtga aatatccaaa ctagccatca
    21181 ataagtaaga ggcagatgta gactgggtca ggtttataat ctgacgaacg tactcgggat
    21241 tcatctgctc cccgattaaa agaagcgaac tttgtgtcaa actcccacca gacttatgca
    21301 tggaaactgc tgccatatca gctcctgcat ccatagcaga aattggaagt ttaccagtga
    21361 aatgcaaatg cgctccatgg gcttcatcta ctaaaaccat catgccagct tcatgagcca
    21421 tttctgtcaa cccctttagg tctgaacaga tgccgtagta agtaggattg ttgattagga
    21481 tagccttagc atctggatgg tcctttatgg cctgtgctac tcggtcattt tcaagaccta
    21541 aagcgatacc aatcttagga tctacactca tctcgatata gatgggaatg gcaccacata
    21601 gaaccaacgc attgatagca gatttatgga catttcgtgg cagaataatc ttatctcctg
    21661 ccttgcaggt tgccagaatc atagtctgca ccgatgaagt tgttccacca atcattagaa
    21721 aggcatggct agctccaaaa gcatctgcag ccagctcctc tgcatcacga ataatcgaaa
    21781 taggatggcc taaattatcc aagggtttca tcgaattgac atcaatgcct acacattttt
    21841 ctcctaagag ttcgacaagt tctggatttc cccgtccacg cttgtgacct ggaacatcaa
    21901 agggaacaat ccttttcttg cgtaacttca ccaaggcctc ataaattggg gcttggtttt
    21961 gatctaactc tttcaaaaca tactcctttc aatattttta ggaccttatg aaaactaagg
    22021 aactaaaggt ggactcatga aaaaagagca ggttttcacc tgctctgcaa tcttctgacg
    22081 tgtttatttt ggtttctgtt gagtatgtct gttcccgatg tttcctaact ctctagtagc
    22141 aaatattacg tactttattg atcgtttcac aagatgacgt gtgctttcac cacactaaaa
    22201 atttatgaat tagaacaagt gtcctaacat caacttataa aagtaggctt ttaaccctat
    22261 caataatgtg gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg taggcatttt
    22321 ttactcgggt ttatatttgc tagaaaacat catctcattt tctaagcttt attactatat
    22381 catgttttta agaacttgta aagtattttt tgataaaaaa ca
//
LOCUS       DAVKJU010000030        22381 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVKJU010000030 DAVKJU010000000
VERSION     DAVKJU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22381
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.030"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(111..314)
                     /gene="blpN"
                     /locus_tag="VW191_001108"
     CDS             complement(111..314)
                     /gene="blpN"
                     /locus_tag="VW191_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEU9623884.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(330..584)
                     /gene="blpM"
                     /locus_tag="VW191_001109"
     CDS             complement(330..584)
                     /gene="blpM"
                     /locus_tag="VW191_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEU9623885.1"
                     /translation="MNTKMMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            876..1454
                     /locus_tag="VW191_001110"
     CDS             876..1454
                     /locus_tag="VW191_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEU9623886.1"
                     /translation="MKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTNKE
                     GTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTKK
                     DYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPSSDF
                     QTKISHCLHCSLKLIGHYYQYRWFLLSTRNLG"
     gene            1381..3024
                     /locus_tag="VW191_001111"
     CDS             1381..3024
                     /locus_tag="VW191_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEU9623887.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            3035..4396
                     /locus_tag="VW191_001112"
     CDS             3035..4396
                     /locus_tag="VW191_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEU9623888.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFINFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            4453..4581
                     /gene="blpC"
                     /locus_tag="VW191_001113"
     CDS             4453..4581
                     /gene="blpC"
                     /locus_tag="VW191_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEU9623889.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(4652..5992)
                     /locus_tag="VW191_001114"
     CDS             complement(4652..5992)
                     /locus_tag="VW191_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9623890.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLHTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(6006..6743)
                     /locus_tag="VW191_001115"
     CDS             complement(6006..6743)
                     /locus_tag="VW191_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623891.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITLNSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRPHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6748..7086)
                     /locus_tag="VW191_001116"
     CDS             complement(6748..7086)
                     /locus_tag="VW191_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9623892.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            7362..7691
                     /locus_tag="VW191_001117"
     CDS             7362..7691
                     /locus_tag="VW191_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEU9623893.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKTETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7859..8908)
                     /locus_tag="VW191_001118"
     CDS             complement(7859..8908)
                     /locus_tag="VW191_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9623894.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQDFPENHWPILLFVGITSVLLFLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLIYVLLLGVGKYFLFRQKASKFFTETG
                     LNWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSILAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQAVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8905..9636)
                     /locus_tag="VW191_001119"
     CDS             complement(8905..9636)
                     /locus_tag="VW191_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9623895.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLTPYSGSININGLTLQEDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAIADLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFNMPEASLNDIYLALTKEEEL"
     gene            9704..10114
                     /locus_tag="VW191_001120"
     CDS             9704..10114
                     /locus_tag="VW191_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9623896.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTPGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10124..10411
                     /locus_tag="VW191_001121"
     CDS             10124..10411
                     /locus_tag="VW191_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623897.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(10791..11927)
                     /gene="dnaJ"
                     /locus_tag="VW191_001122"
     CDS             complement(10791..11927)
                     /gene="dnaJ"
                     /locus_tag="VW191_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU9623898.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12200..12556)
                     /locus_tag="VW191_001123"
     CDS             complement(12200..12556)
                     /locus_tag="VW191_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEU9623899.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKGFIDNIPVECLTRY
                     REIILAEHNSCIKNDEAVRNFVTSVLLSAFVSAIVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12558..14381)
                     /gene="dnaK"
                     /locus_tag="VW191_001124"
     CDS             complement(12558..14381)
                     /gene="dnaK"
                     /locus_tag="VW191_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEU9623900.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVKRIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14850..15374)
                     /gene="grpE"
                     /locus_tag="VW191_001125"
     CDS             complement(14850..15374)
                     /gene="grpE"
                     /locus_tag="VW191_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU9623901.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15401..16435)
                     /gene="hrcA"
                     /locus_tag="VW191_001126"
     CDS             complement(15401..16435)
                     /gene="hrcA"
                     /locus_tag="VW191_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU9623902.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16599..17108)
                     /locus_tag="VW191_001127"
     CDS             complement(16599..17108)
                     /locus_tag="VW191_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEU9623903.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLSGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17715..20048
                     /locus_tag="VW191_001128"
     CDS             17715..20048
                     /locus_tag="VW191_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU9623904.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKVIDYFSSATQIGMTATPKETKN
                     VSNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20061..21524
                     /locus_tag="VW191_001129"
     CDS             20061..21524
                     /locus_tag="VW191_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEU9623905.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNSVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21524..>22381
                     /locus_tag="VW191_001130"
     CDS             21524..>22381
                     /locus_tag="VW191_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9623906.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIADTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLAEQQRIIEAIESALEKVDEYAESYNRLEQLDKKFPDKLKK
                     SILQYAMQGK"
BASE COUNT         7124 a         4529 c         4097 g         6631 t
ORIGIN      
        1 atagtctttt gttttttatc agagatactt atggatagat aaataagata ggtttgaaaa
       61 gcgaagagaa taataaagaa tatagccttc ataaaattta gctttcattt ttatgatgta
      121 gcggtatagg ctaaatatcc acaaaccact gctcctccaa ttcctcctat tgcagcgccc
      181 catggtccta gaattttccc atatttcact ccacccgctg cacaacctaa agcagcaact
      241 acagctgctc ctccggaatt acctccgtaa acctcactca gcattgtttc atttatatta
      301 caataagtat tcatacaagt ctccttttgt taaaatccac ccgttgcccc tgttactcct
      361 gcccaaagag ccacaccaaa tttagctcct atgtatccac atgctcccat aaatggcgct
      421 ccaacaccac tcgcagcaca aatagctgtc cctaaccccc agccaccaaa agcagcacca
      481 ccaccttcta agacattagt ttgccaatta ttcttacctc cttcaatact agataacata
      541 gttatatcca tttcatgaaa ttgttccatc atttttgtat tcatgacaaa tactcctttt
      601 ttcttttttt atttttgtct tgttgtaact ttgataagtt tagtatatca tcgtttttta
      661 aaatttttca tccagatctt gaattgtcat cgaaacgtct tgaattagct tttttatttc
      721 aagccacctc taaatgttta aaaaaaataa tttctaatca cttttttacc attcaggaag
      781 ttttaatgac tattcaagat ttcataaaat atgaacttag ttttatgaca taatagacct
      841 atccactata tgaaaggaat tgccaatgac ttcttattaa acgtacattt gttcctcaaa
      901 tagatgcgag agactgtggt gtcgctgcct tagcctcgat tgctaaattc tatggttcag
      961 atttttctct agctcacttg agagaacttg caaagaccaa taaagaaggg acgactgctc
     1021 ttggcattgt aaaagccgct gatgaaatgg gctttgaaac aagacctgtt caagcagata
     1081 aaacgctctt tgacatgagt gatgtcccct atccatttat cgttcacgtt aacaaagaag
     1141 gaaaactcca acattactat gttgtctatc aaacaaagaa agactatctg attattggtg
     1201 atcctgaccc ttctgtaaaa atcactaaaa tgtcaaaaga acgctttttc tatgaatgga
     1261 ctggagtagc tatttttcta gctaccaaac ccagctatca accccataaa gataaaaaga
     1321 atggtctact aagcaagctt ccttcctctg attttcaaac aaaaatctct cattgcttac
     1381 attgttctct caagcttatt ggtcactatt atcaatatag gtggttctta ctatctacaa
     1441 ggaatcttgg atgaatacat tccaaatcag atgaaatcaa ctttaggaat catctcagtt
     1501 ggtctggtta tcacctatat cctccaacaa gtcatgagct tctccagaga ttatctccta
     1561 accgttctga gtcagagatt aagtattgat gtgattttat cctatattcg ccatattttt
     1621 gaacttccca tgtctttctt tgcgacacgt cgtacaggag aaatcatttc acgattcaca
     1681 gatgctaact ctattataga tgccttggct tctaccattc tttctctttt tctggatgtt
     1741 tctattctga ttcttgtagg aggcgtctta ctggcacaaa accctaatct cttccttctt
     1801 tctcttattt ccattcctat atacatgttc atcatctttt cttttatgaa acctttcgaa
     1861 aaaatgaacc atgatgtcat gcaaagtaat tctatggtta gctctgccat tatcgaagat
     1921 atcaacggga ttgaaactat aaagtcgctc acgagtgaag aaaatcgcta tcaaaatata
     1981 gacagcgaat ttgtagatta tttggaaaaa tcctttaagc tcagtaaata ttctatttta
     2041 caaacgagtt taaagcaggg aacaaaatta gttctgaata tccttatcct atggtttggc
     2101 gctcaattag tcatgtcaag taaaatttct atcggtcagc tgattacctt taacacactt
     2161 ttttcttact ttacaactcc tatggaaaat attatcaacc tccaaaccaa actccaatct
     2221 gcgaaggtcg ctaataaccg tttgaacgaa gtctatctag tcgaatctga atttcaagtt
     2281 caagaaaacc ctgttcattc acattttttg atgggcgata ttgaatttga tgacctttct
     2341 tataagtatg gttttggacg agatacctta acagatatta atctcacgat taaacaagga
     2401 gataaggtta gcctagttgg agttagtggt tctggtaaaa caactttagc caaaatgatt
     2461 gtcaatttct ttgaacccta caaaggacat atttccatca atcatcagga tattaaaaac
     2521 attgataaaa aagtcttgcg ccgtcatatt aattacctac cacaacaagc ctatatcttt
     2581 aatggctcta tcttggaaaa cttaactttg ggcggtaatc atatgattag ccaagaagat
     2641 attctaagag cttgtgaatt agctgaaatt cgtcaagaca ttgaaagaat gcctatgggc
     2701 tatcaaactc agctctctga tggagctggt ctatcaggag gacaaaagca acgaatagcc
     2761 ctcgctcgtg ctcttttaac taaatctcct gttttaatac tagacgaagc tactagcggt
     2821 cttgatgtct tgactgagaa aaaggttata gataatctta tgtctctaac tgataaaacc
     2881 attctctttg tagcccatcg tctcagtata gccgaacgaa ccaaccgtgt cattgttctt
     2941 gaccagggga aaatcattga agttggtagt caccaagagt taatgcaggc gcaaggtttc
     3001 taccatcatc tattcaataa ataaggagaa tgtcatgaat cctaatcttt ttagaagcgt
     3061 cgagttttat cagagacgtt accataacta tgcgacagtg ttaattatac ctctttcatt
     3121 actatttact ttcatcttga ttttctccct tgttgccaca aaagaaatta ctgttacttc
     3181 ccaaggagaa atcgccccta caagtgtcat tgcctccatt cagtcaacca gtgataatcc
     3241 tatcctagct aatcatttag tggcaaatca agtagttgaa aaaggggact tactcatcaa
     3301 atactctgaa acaatggaag aaagtcagaa aactgcctta gcaactcaat tacaaagact
     3361 tgagaagcaa aaagaaggac ttggaatttt gaaacaaagc ttagaaaaag cgactgatct
     3421 tttttctggc gaggatgaat ttggctacca taataccttt ataaatttta ctaaacaatc
     3481 ccatgatatt gaactgggta tcacaaagac taacaccgaa gtttcaaatc aagctaatct
     3541 ttccaatagc agttcatcag ctattgaaca agaaattaca aaagttcaac aacaaattgg
     3601 agaatatcaa gagttgagag atgctatcat aaataacaga gcacgcttac caactggcaa
     3661 tccgcaccag tcaattttga atcgttatct tgtagcctca caaggacaaa cacaaggaac
     3721 tgcagaggag ccatttttat ctcaaattaa tcaaagtatt gcaggtcttg aatcatctat
     3781 cgcaagcctc aaaattcagc aagctggtat cggaagtgta gcaacttatg ataacagttt
     3841 agcaaccaaa attgaagtac tccgcactca gtttttacag acagcctcac agcaacaact
     3901 aactgtggag aatcaattaa cagaattaaa agtacaacta gatcaagcga cacagcgctt
     3961 agaaaataat accttaaccg ctccaagtaa aggtatcgtt catctgaaca gcgaatttga
     4021 aggtaaaaat agaattccaa ctggtacaga aattgctcaa atattccctg tcatcacaga
     4081 tacaagagaa gtactaatca cttactacgt atcatctgac tatctcccct tactagataa
     4141 aggacaaact gtaagattaa aactggagaa gattggaaat cacggcacca ccatcatcgg
     4201 ccaacttcag agtattgatc aaactcctac cagaacagag caaggcaatc tctttaaatt
     4261 aaccgctctt gcaaaactat ctaacgagga tagtaaactc atccaatatg gcttacaagg
     4321 tcgcgtcact agtgtaacta caaagaaaac atattttgat tatttcaaag ataaaatttt
     4381 aacacattct gattaatttt cagataacac tctataacta tttattatct tatcaaaaag
     4441 gagaatcata acatggataa gaaacaaaac ctaacttcat ttcaagaact aacaactacc
     4501 gaactcaacc aaattacagg tggaggattg tgggaagatt tattatataa cattaataga
     4561 tatgctcatt acatcacata agaacttcat catccaatac aactataaaa aaataagacc
     4621 gagaaacaag tactctcggt cttatttttc atcattctgt atgtatcaca gtaagtacct
     4681 gacgaaagac ttgattttga caggtggtat ttagactggt attaggatgg ctttccacaa
     4741 tcttcatgac ggtatggaga ccaactcctc tctcctcccc tttagaactg gctccaaagg
     4801 agaagatttc agaaatatcg atgccctctt ctttgatgga gttttcgatg ataaaggtct
     4861 cctgtgctcc attttttaaa aaggcgattg aaacatgagg ttgactagct tccacactgg
     4921 cttcaatagc attgtcacaa aggatagaca caatggttag aaaatcaagt agactcatcc
     4981 cctcgacctg aatctcctca ggaacttcga cattaaagac aatgttctta tctctggctt
     5041 ttaaaaattt ccctgctaga agacttttga gggctttatc acgaatattt accaatctgc
     5101 ccaggtcata tttattgttc tgcaatttct gactggaatc ctttaagacg gagccataga
     5161 cctcttttat ctgctccata tcctcctctt caatgcccag acgtaagcta gtcaagaggt
     5221 tggtataatc atgacgaaag ctccgtactt ccttgtaaag ctcctctata tgccgactat
     5281 agcgttccat atctctatag cgcagggcct gctcttgttc caatctctca tagagttttt
     5341 ccttcaaata ggtatccaat ttcttgataa cccccataaa aaagagtagg taaaagacta
     5401 ggatgagatg gcgaacagtc tttgattgaa tactttgttc atattcaaaa aaagacagac
     5461 tttccatgac tagatagtag ccacccatta tccagttaat ctgagtcagg gacttttgaa
     5521 aggctttatc gagaatctcc tttctcaagc tagtaaaatc gtagtccaac catttcaaaa
     5581 aagctagaga aatgaagaaa ttgaaaatta ttatacataa cccagtaaat gagtagccat
     5641 catatacttg cccttgtccc aaaaatggaa gcacaaaata ggagactcct ctataaaaga
     5701 gattcaccaa tatcattgga aagagaccat aaaagaaaag gagtttttta ggaagccctc
     5761 tcaataataa gaaagataag cctatgccgt acaagggttc cataaaataa gataggtaaa
     5821 catttcctac tatatagcta atcatcacaa aaacaaaggc caacagtatc ttaaaaagaa
     5881 aggccttaaa aatcctctcg aaagtaagat caattccatc caccttaaag aagatgacaa
     5941 tttctagtcc attagtaaca agtgtataca acaatatcca agcaatgttc ataaattctc
     6001 ctagctcagt gtaatttatt gatggcctca gacacttccc tgaccttata acgcgcgatc
     6061 agacaacttc caccattggg aaagaaaagg agtttttctt tcttatccaa atgcaccaca
     6121 tttgcaggat tgatgagaaa agagcgatgg cactgcaaga gacggggttc ctgcttgaaa
     6181 acctcctcta aactcgctgt aaattccagc ctgtctgtct tggtatagag aataacacga
     6241 tggggtcttg gggatgtttc gagatagtaa acctctttaa aaggatactg aaattgggca
     6301 aattttgatt taaagtaaaa gcaatcttcc gccagacttt tactatcttg actattggca
     6361 tagaggagag ctgtctcgat tcgagattca aactcctctg ccgaaagggc cttatcaata
     6421 tagtccagag cagacacttg gtagcgaaag gacaggggca taaactccga gtgagtcgtc
     6481 acaaagacaa tcagggcata cggatcccga tcccgaatct ttctagccac ttccagaccc
     6541 ttcatctcct catttcgaat ctcaatatcc aaaaagaata gctgatgggc ccccttctca
     6601 tgcacctctg ccagcaattg atctggctta ccaaaaactt caaaagaatt cagagtgata
     6661 tgatgttcct tcaagagttt ctcaatcgtc gtttcaattc tagtctgttg ggaaaaatca
     6721 tcttctaaaa caaatattct catcttctta ctctccttct ttcaaccatt tttgacgaat
     6781 ttctctatag cgacgtctgg catacttgac agcgtattga ccttcttctc caagaaaaag
     6841 gattctttct tgaaaatcaa tcgattttac tttatcaaga ttgaccaaac aatttcgatg
     6901 acatctcatc aaggtttccc catattggtt ctcaagatta cttaaatttt gaagcatatt
     6961 aaaactagct ttttctgtaa caatctgtac ggtatgggct ttagttggat gtgtttggat
     7021 atagtagata tcgtcgatgt ttactttata gactgttttc tgcgtctgaa taatcatatg
     7081 tttcattgga ttctcctcct aagaaaagaa taacagaaaa gttctagccc aagcccaccc
     7141 aatcctaaac agtctttgaa aactcctaaa aggattattt tctacaacat catgatgagc
     7201 ttacaactat tttatcataa aattcactca gcgcaattca agacatttca atgacaatta
     7261 aagatttgaa taaaaaaaaa gaattaaaag tgataaacta acagtatcaa aacacagttg
     7321 ccagaagtaa gactggcacc cagattaaag gaggcattcc catgacagat acagacccca
     7381 tcaaaagagc tcatactttg attactgact taaacaaagc ctaccaagca tgcaaacagg
     7441 caagcgctga cgacgtccgc tttcaggagc aattaaactc tattcttggt tttctagcca
     7501 agactgaaac agtggataat cgattcttga ttgaactaga aaaattttac cagacttcca
     7561 gtcttctcat gggactcagc gctcttgatc cagatgctcc aactcgcgcc gcttggcggg
     7621 cctatgaccg cttccacttt gaccaagtca agaccaagtt aatactcaat gagaatcaaa
     7681 gagcaaacta ggaagctagc cgcaagctgt acttgagtac ggtaaggcga cgctgacgtg
     7741 gtttgaagag attttcgaag agtataagtc tctacggacc aaccattatc ctgtagtaac
     7801 aaaaaagaag ctgagacaaa ctgaactcaa cttctttttt agtgtcatat aggcaagatt
     7861 aatcctgcat ctgacgtttt acctgataag gcaaatacaa gactagtaaa accaaaccag
     7921 ctcccagtaa ggctagaagg gctagttttt cttggaaggt aatcaaccca acaactaatt
     7981 ccaccagtaa aacaaaactg gtcaatcctc ggactacagc ctgtaagcct ttttcctttt
     8041 gccccttgtc cagtggaaag agttgggtca aatactggta gtcaaaggca tgatagaggg
     8101 ccagcaactg aaagagcaag aggtagttaa agagaaccac cactgctgtc gcaatccaag
     8161 cttgctcgat aaatacctgc gccagtatgg aaagcaagag cagacggaga ctgagcgcaa
     8221 agaggtctcc atttcgcaga taagaacgta gatagagatt ttgccaaatc tttccaggca
     8281 ccttctgaac agcctttagg ataaagtcca gataggcacg acgcttgacg ctgtttgaaa
     8341 ttcccttgac ctgcgtaaag agggcaaaga aacgaagcaa gacttgcttg cgcttgcttt
     8401 cttgagaaat aacatagtcc cagttcagtc cagtttccgt gaaaaatttg ctggcctttt
     8461 gacgaaagag gaaatatttt cctactccca ataaaagcac atagattagg aagacaggca
     8521 aaccataacc catggctaaa aataagggcg caaataacag caagaaaagg gtctggacaa
     8581 agagccaaaa gactagggaa atgccagttt gacgcttgag atggagtttg atttcctctt
     8641 ctccgactaa gagaaagagc ttgtctggag cctccatata ggtggcaatt ccaccccaaa
     8701 gaaaaagtaa aacagaggta attcctacaa ataaaaggat aggccaatga ttttcaggaa
     8761 aatcttgcaa gagttgactg tactggtagg ctagaaagcc cagcaagaca agcaggaaca
     8821 agacaaagtg gtcattgaga acatagcgca gataaccaag acactcctta cgaaaagcct
     8881 gctttctctt taaaaacaag tctttcatag ctcctcctct ttggtcagag ccaagtaaat
     8941 atcattcaaa ctagcctcag gcatgttaaa ggcttcgcgc agttgcagga gatttccctt
     9001 ggcacgcacc tctcccttat gaagaataac aaaggcatca cacatcttct ccgccgaatc
     9061 cagcacgtgg gtactcataa gaatggactt gcccttttgc ttttccactt ccaaaagctg
     9121 aatcaagtct gcaatcgcca gcggatccag accaaggaaa ggctcatcca cgatgaaaag
     9181 actcggatct accacaaaag cacagataat catgaccttc tgcttcatcc cttttgaaaa
     9241 atgaacagga aaccagtcta atttctggtc cagacggaac atttttaaca agggctctac
     9301 tcgctcaaaa gccactttct gctcaatacc ataagccatg gcaaccgttt cgatatgctc
     9361 tctgagggtc aattcctcat acagactagg tgtctcagga atgtagccaa tttgcttgcg
     9421 gtaactagtc gcatcttctt gcagggtcag gccattgata ttgatggaac cactataagg
     9481 tgtcaacaga ccgataatct cattaatcgt cgtcgatttc ccagcaccat tgagaccaat
     9541 caaaccaacc aactgcccac tttcaacagt aaaagacaca tctttcaaga cagggacatg
     9601 aacatagcca cctgtcaggt ttttaatttc taacatattt tctccaaatc tggtataatg
     9661 tagctatatt atatcaaaat tcaatacagt agaggtagat tttatgtcag attgcatttt
     9721 ttgtaaaatc atcgcagggg aaattcctgc ttcaaaagta tatgaagatg agcaggttct
     9781 tgcctttctt gatatctctc aagtgacacc aggacacacc ttggtcgtgc ccaaagaaca
     9841 ctatcgcaat cttttggaga tggatgctac gagcgccagc caactctttg cccaagtacc
     9901 aaaagtagct caaaaagtca tgaaagtcac taaggctgct ggtatgaata tcatttccaa
     9961 ctgtgaagaa gtcgctggtc aaacagtttt tcatactcac gttcaccttg tgcctcgcta
    10021 cagtgctgac gatgacctca agattgattt tatcgcccac gaaccagact ttgacaaact
    10081 tgctcaagtc gctgaaacca tcaaaaacgc ttaaggagtt gctatgaaat tatccaacct
    10141 actgctattt gcaggagctg cagccggaag ttatctggtt acaaaaaatc gccaaaccat
    10201 cacagatgaa gtcttgaata ccactgaccg cgttcaagct atcaaggacg atgtggatat
    10261 tatccaaaat agcctgcaaa tcatcgacca gcaaaaagaa cttatcaagg aataccaaga
    10321 agacttgact tacaagttta aggtcttgga aaaggatatc cagactagac tagctgtgat
    10381 aaaagaaatg cagggaactg aagataagta aaaagagccc cccggctctt ttttaaaacg
    10441 taaacaagtt atagtgaatt gaatttagaa tagcacacca tagtttctaa agcattgtta
    10501 gagattactt taaaatcctc ttatcaattt gttcatattc tatttcaatc tattatatat
    10561 atcccatcaa ctatgcatca taatttaggt aactcatact caataaaaat caaaaagcaa
    10621 actagaaagc taggcacaga ctgctcaaaa caccgtttta aggttgtgga tagaactgac
    10681 gaagtcagct caaaacactg ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat
    10741 acccatgaca agccgacgct gacgtggttt gaagaaattt ttgaagagta ttattctcca
    10801 tcaaaggcat ctttaatatg gtcaaagaag ccttttttct ttggatttac tttcaagtca
    10861 ccagcagccg cgaattcttt caaggctact ttttggcggt cgttcaagcc tgtcggtgtt
    10921 acgacattaa cagtaacgta ttggtcacca actgcaccgc cacgaaggct cggtgccccc
    10981 ttactacgta ggcggaattt cttaccagtc tgagttccct ctggaataac caattcaaca
    11041 tcaccgtgaa cagttggaat atctactgta tcaccaagag ccgcttggac aaagttgagg
    11101 ttgagattgt agaagatagt cgttccttca cgttcaaact tgtcgctagc ttctacagaa
    11161 actactacat acaagtcacc ataaggtcca ccgttaaagc ctgcttcacc ttgaccagcg
    11221 aggcgaattt gttgacctgt ttccacacca gcagggattt tcacatgtac gctatgagct
    11281 tgtttctcat gacctgttcc atgacaggtt gtacatggat atttgatttc ttttcctcga
    11341 ccgtgacaga catcacaggt tacttggcga cgcatcatac caagaggagt ctgcgtatcg
    11401 acgttaatga caccagcgcc atgacagcgt ccacaagtga ctggacttgt ccctggctta
    11461 gcaccagagc cattacatgt acgacagcca gcttcacgat gatacttaac ttccttctca
    11521 gttccgaaga tagcttcttc aaaggtcaaa ttgacacgat actggagatc atctccttgg
    11581 cgaggagcgt ttggattgcg cgaagaaccg cctccgccga agaaacttga gaaaatatcc
    11641 tcaaaaccac cgaaaccacc tgccccattg aaaccgccga aaccaccagc tccaccaaaa
    11701 ccaccattgg cgcctgcagc accatactgg tcataggcag cacgtttttg gtcgtcactc
    11761 aaagtctcat aggcttcttg aacttccttg tacttgtcct cagcaccagg ctccttgttg
    11821 atatctgggt gatatttttt ggaaagctta cgataagcct ttttgatttc gtctgccgaa
    11881 gcgtttttgg atacccccag acgatcataa aattcagtat tgttcataca agataccaag
    11941 accgtaaaat tcgactgaaa aataggaaat ctgacgctgg agcgacgctc ctagacagat
    12001 ttctcttttt tccgagaatt taggtcgggt tcaattcctt tcttttatat tgatgagata
    12061 atactccagc cactcggccc tatccctttt tatcgaaaaa cagaggctgg gttgcaacct
    12121 ctgcaaataa gtaaattaac tttattttga ttttaaatat cctataagta cagaaatatt
    12181 tatatatttt tgtctcttct tagtatttgc ctatataatt ccaattgatc ataagaaata
    12241 caactactgt ccaaatcata ccaattatga acggtattac aaatttatat gtttgtattt
    12301 ctaatgatat catagctgat actatcgccg atacaaatgc agacaataat actgaggtaa
    12361 caaaattcct taccgcttca tcatttttga tacaactatt atgttcagct aatataattt
    12421 ctctatatct agtcaaacat tctactggaa tattatctat aaaacctttt gatgttctat
    12481 agttatcagc ttgattacaa aaatcattat aaacttttcg tgtatttttt agatactctt
    12541 catccaatac actcatctta cttttccgta aactctccgt ctacgacgtc atcgcctgcg
    12601 tttcctgttg cttgtgcgcc ttctgctcct tcttgagctt gttgcgctgc tgcggcttgt
    12661 tcgtagagtt taacagcaag tccttgagct ttttcgttca atgcttcaag ttttgctttc
    12721 atgtcgtcca agttgttgtc ttcttgagct ttcttaaggt catcaagggc agcttgggca
    12781 gcgtcacgtt ctgcgtcgaa gcctttacct tcagtttcct tgattgtctt ttcagtcgca
    12841 aagattgctt ggtctacttc attacgaagg tctacttctt ctttacgttt cttatcggct
    12901 tcagcgtttg cttctgcatc tttcatcatg cggtcgattt cttcgtcagt caaacctgag
    12961 ttcgattgga tgacaatagt ttgttctttt tgagttccaa ggtctttggc cttaacagac
    13021 acgataccgt tcttgtcgat gtcaaatgtt acttcgattt gaggaattcc acgaggtgca
    13081 gctgggatat cagtcaattg gaagcgtcca agagtcttgt tatctgctgc cattgggcgt
    13141 tcaccttgaa gaacgtggat atcaacggct ggttggttgt ctgctgctgt tgagaagact
    13201 tgtgatttag atgttgggat tgtagtgttg cgatcgataa gttttgtaaa tactccaccc
    13261 attgtttcga taccaagtga caatggcgtt acatcaagaa ggacaacgtc cttgacatca
    13321 ccagtaatca caccaccttg gatagccgca cccatagcaa ctacttcatc agggtttact
    13381 gatttgtttg gttctttacc agtttcagct ttaacagctt caacaacggc agggatacga
    13441 gttgaaccac caacaaggat aacttcgtcg atttctgaca agctcaaacc tgcatctgaa
    13501 agggcttgac gaactggaac ttttgtacgt tcaacaaggt cacgagtcaa atcatcaaat
    13561 ttcgcacgag tcaaagtcat ttccaagtga agaggtccag cctcacctgc agtgataaat
    13621 ggcaagctga tttgtgttga agttacacca gaaaggtctt tcttcgcttt ttcagccgca
    13681 tctttcaaac gttgcattgc catcttgtca gtagacaagt cgataccgtt ttctttcttg
    13741 aattctgcta ccaagtggtc aatgattttt tggtcaaagt cgtcaccacc aagtttgttg
    13801 tcccctgcag ttgacaatac gtcgaagaca ccgtcaccca attcaaggat agagacgtcg
    13861 aatgtaccac caccaaggtc aaataccaag attttttctt ctttgtcagt cttgtccaaa
    13921 ccataagcaa gagctgctgc agttggttcg ttaacaatac gttttacttc aagaccagca
    13981 attttaccag cgtcttttgt tgcttgacgt tgagcgtcgt tgaagtaagc cggaactgtg
    14041 ataacagctt tggttacttt ctcaccaagg tagtcttcag cgtagccttt caagtattga
    14101 aggatcatag ctgagatttc ttgtggagtg tattcttttc catttgcaga aactttttca
    14161 gaagttccca tcttagattt gatagagata actgtatctg ggtttgtgac tgcttgacgt
    14221 tttgcagcat caccaacgat gatttctccg tttttgaatg agactacaga tggagttgtg
    14281 cggtttcctt ctgggtttgc gatgattttg ctttcagttc cttcaagaac tgcaactgct
    14341 gagtttgttg tacctaagtc aataccgata attttagaca tgtgtttttc tccttgttag
    14401 ttaattttct atttttcttt ttgatactct tcttaagtgg cgacgccgtc agagcttgac
    14461 ttcgtcaatc tctttgacta aactttgagc ctaaggtctc aaaatttgcg caaatagcgc
    14521 cacggcgaag agtatcgtct ttggttcgct tcgctcacta ttacaaagcc aaacatattt
    14581 tatctttctt catagtttat tgtcgtttcg gacaagtttt ctattatgta aattgcgaca
    14641 cgaggagtcg aaatcgattt tatttcgacg acgagtttgt aaggaagcta gacaaacgcc
    14701 atagcgattg ccgttttctg acgagttgct ttagctaccg tcagaattgc cgaatcgaac
    14761 acgccctaag ctctgagtaa aaaagataga ttttcttgtg ttcatcgaac acttcgtcaa
    14821 tctcctattt ttactgcggg ctttgtatct tagttataca ccactaccat tgctgggcgt
    14881 aggatgcggt catggagttt gtagcctttt tgaaagactt gggcgatggt atctactggg
    14941 tgttcatcgt ctgctgggag agtttggatg gccatatggt agttatggtc aaattcgcca
    15001 tctgctgcga tttcttcaat tccttcttct ttcaaagcgt gaatcaagct ttcttgcacc
    15061 atccccaagc ccttcttcac atcatctgtc aaaccttcaa ctgcaagtgc acgctcaagg
    15121 ttgtcaagag atggtaagat tgcttttgcc aagtcctggc tacgataacg ttgcaagttt
    15181 tgacgttctt cattggcacg gcgttggata ttttgcattt ctgcatgagc gcgaagatat
    15241 ttgttttcga actcatctgc acgttcattt gccaagtcca actcagactt ttcaggagtt
    15301 gtttcttcag ctgtttccac aacttcctct tcttgaactt cttctacttc ttcatttttt
    15361 atatcttggg ccatgttcgc ctcctttaat gatttcaatc ttaatgtact tcataatgat
    15421 tactgctgag gtagcggtaa aaatctgtca acttcatggt caaaacacgg ttgaccacat
    15481 tgacttgatt gattagctgt tggtaatcca gattaactgg accgataatg gctagaattc
    15541 caactccccg ataaggaatg aggaacttac tactaatcac cgctaggtca gctaaacagg
    15601 actcttgacc gtctgcaaca cgaacatttt gcatctgatc ctcacgcaac ccctcacgaa
    15661 tctccaaggc caccttttgc ggttggtcaa agaactgata ggctgctaga ttggcaaaat
    15721 tcaagagatg gaccttgccc gccatcacaa tgttttcgtt gaacatttcc ttaaagatgt
    15781 gttcaaagag atcgatgaca ttatccgttg ttgtaaagta acgctggata atctgcggaa
    15841 tctccgtccg aatcttgtag tgaatatcta aaacggtgtg accgaggaaa cgttcctgaa
    15901 tgatgctctt cagtttcagc aaatcctcct gcaagaagtt ccttggaatc agaaactgac
    15961 tagtaaccgt tcgcgactcg tctagggtaa ataccgccaa ggctgtatgt tgccccaaaa
    16021 caacgatatc aaaggctgtc aaacgttgcc tgctcggctc aacatccagt gccactaccg
    16081 tacagccact caggtctgtt agcaagttag cagcctcttg cagaatatcc tccaatttga
    16141 agaattcttg atcaaaggct ttgacaatct catatacctc attttcagcc agtcggtcaa
    16201 aatccagtga gtgtttcaca tagtactgaa aaccagcaac acttggcatc cgaccgcttg
    16261 aagtatgagc cttctcaagc aacccttgtt tttctagttc cgccatgtca ttacgaatgg
    16321 ttgcactgct agagttaata gactcttgca aggtttttga tccgacaggt tcgtgcgttt
    16381 tggtaaagat gtcaataatc agatttaaaa tatcctgctg acgctctgta accatctcat
    16441 cactcctctc tgtctgatca attagcactc aatgcatcca agtgctaact tatgattcta
    16501 ttatacaccc ttagaagaga atgtcaagac aaaaactcaa aaaattagca ctcttttatc
    16561 aagagtgcta aaaatcaatc tgaagaccta gaaccttgct attcatctac tcggtatttc
    16621 ctttttagtc tgaaataacc atgaattaga taagaaatca gaaaagcata taaaacaaaa
    16681 ataagaaaga aagacataga aagatcctct ctaaacaaac tcatacaaac aacaagacca
    16741 cttaagaaag tagctgtaca ggaacgaagt ctagatcgca taacttccca tttgagcccc
    16801 ttactcatat agacttgatc ctctggaagt aaatcagaag acgatttacg aagaatcgaa
    16861 caagaacctg ctcctatcaa ttcccaacct ctatctctaa aatcttgcag ttcatgctta
    16921 tattttttaa gaaatctaga atcatagata cggtagatga catcgtctgg ttgacattgg
    16981 tcaaaataga acaaaccaaa acgactcgtt ctatacctcc aacctttcaa atgcatctca
    17041 tgtaaatatt cttcttcctt gtccaaatca acaatagtga aaatccgaaa ttctactctg
    17101 ctattcattg tcttacccca aaattagaaa acatgcctgg cgttatttat tagataattc
    17161 tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcgcaa aaactatcaa
    17221 taaaacccaa tctattatga aagtcaaaaa cacttcccaa ctgaaagaac tacctccagt
    17281 gacaaacttt gagaaaaacg gtagtagagc taaaaagaga agcaaaatag gaagcatccg
    17341 cattgttaaa atccttttgg cataaaaaaa tctttattta aacgaaaata ttatggcaaa
    17401 atttacgcca gtttttgaac ggctgatgta gatattttat actttcaaaa tgtttaaatg
    17461 tgattattta tttttgaaaa atagctcacc agcccgactg aaagtgctta taaatgataa
    17521 taagtcgcct gccgaaaaca gcgaaaaata gcggtgttat gcggagataa tctgacgcga
    17581 tgcgaaagta tattgcatac ttattttcaa caatttagca gagtattttt ataagtgtga
    17641 tataatagaa gtataatttg ttctgatagt ttattttatg gagaagtaga tttttagaat
    17701 gcggagggtt caatatggtt gagtttataa agtctaagaa agaaatgagt gaggaggata
    17761 ttaaagcaaa tttcatcact cctgctattg tatccaaagg atggaaaaat ggtgagcata
    17821 tcgcttacga agaatacttc actgatggtc gaatcgaagt tagaggagat aaggctcgtc
    17881 gtaaagaagg aaaaaaatca gactattcac tgtattacca atttggaact cgaattgcaa
    17941 ttgttgaggc aaaggataat aaacacagcg ttcgagcagg attacaacaa gctattgaat
    18001 atggagagat tttagatgtt ccatttgttt attcttcgaa tggtgatggc tttattgaac
    18061 acgaccgtat cacgagagaa gaacgtgagc tggagttaga cgaattccct actcgtgaag
    18121 aattattttc tcgtatgacg aaggaaaaag gattgacgta cgaaattaca gaagctatct
    18181 caactccata ctatacagac gccttctcaa tgaaaacgcc acgctattat cagcaaatag
    18241 ctatcaaccg tactattgaa acagttgcca gaggacaaaa acgagtaatg tttgtgatgg
    18301 caacaggaac ggggaaaacg ttcatggctt ttcaaattat tcatcgcctt cgaaaagctg
    18361 gtttggctaa acgagtttta ttcttagcag atagaaacat cttagtagac caaacgatgg
    18421 ctgaagactt taggccattc gaaaaggtaa tgacgaaaat tacaccaaaa cttttgactg
    18481 ctcctgaaaa attaaattct tttgaaattt atctagggct ttatcagcaa ctaactggtg
    18541 aagatggaac tgaaacacat tatcaaaaat ttgacaaaga cttctttgat ttaatcgtaa
    18601 ttgatgaagc gcaccgtggt tcagctaagg aaaacagtaa ctggcgtaag gtaattgatt
    18661 atttcagttc tgcgacacag attgggatga ccgctactcc taaagaaacc aagaatgttt
    18721 ccaatacgga atactttggt gagccaatct atacttatag tttaaaacag ggaatcgagg
    18781 atggtttttt ggctccatat cgtgttatga gggttaattt agatgtggat gtggatggtt
    18841 atcgtccaga aactggaaaa gttgatgcta acggacaatt aatagaagat aggtactacg
    18901 gcaggaaaga ttttgataaa accattgtca ttgatgatag aacgcaaaga gttgccaagt
    18961 ttgtttctga ttatatgaag caaaacaatg cacgatttga taaaacaatt gttttttgtg
    19021 ttgatattga ccatgccgag cgaatgcgtg ctgcacttgt aaaagagaat ctagacttag
    19081 tccaagaaga ctatcgttat gtcatgcaag taactggtga caacgctgaa ggaaaagctc
    19141 aactggataa ctttatggat gtcaattcta attttcccgc tattgtaaca acgtctaaat
    19201 tattaacgac aggagttaat gctaaaacat gtcgtttgat tgttttagac tctaatatcc
    19261 aatccatgac tgaatttaaa caaattattg gtcgtggcac acgtctttat cctcaaaagg
    19321 ggaaagaatt ttttacgatt attgattttc gaaatgttac caatttgttt gctgaccctg
    19381 attttgatgg tgatccagtg aaggtgctgg aaacaggtgc gaaaacagtc agtggttcta
    19441 cgcccggttt cgtagatgag gaaggtgacc cagtagaaaa atatatcgtt acagacaagc
    19501 aggttaccat tcttaattct actgttcaag tattggatga aaacgggaaa ctgattaccg
    19561 aaagcctgac cgactacact cgaaagaata tcttaggtag ctacgccact ttgaacgatt
    19621 ttatcacagt ttggcatacg gcagataaga agaagcttat cttagacgaa ctttataaaa
    19681 aaggagttta tctagatgct attcgagagt cggagggaat atcagaacaa gaaatcgatg
    19741 attttgattt actcctaaaa cttgcctatg gtcaaaaaga attaaccaaa acggaacgta
    19801 tcaataaact caaacaaagc ggatatttat ataaatatag tgaggaagcg cgtgctgttt
    19861 tggaaatttt actgaacaaa tacatggata aaggtattgg agaactcgaa agcattgaaa
    19921 cattaaaact tccagaattt cagatatatg gtggaacctt caaaatcatc aatacttatt
    19981 ttggagataa aaaacgatat ttacaagcaa ttaaagaatt ggagcaagag ctatttacag
    20041 tagcttaatg aaaggaaagt atgtcaatta catcatttgt aaaaagaatt caagatatca
    20101 ctcgaaacga tgctggtgtt aatggtgatg ctcaacgtat tgagcaaatg tcttggttat
    20161 tattcttaaa aatttatgat agccgtgaaa tggtttggga attagaagaa gacgagtatg
    20221 agtcaattat cccagaggaa ttaaaatggc gaaattgggc tcatgctcaa aatggggaac
    20281 gggtattgac aggcgatgaa ttacttgatt ttgtcaataa caagttattc aaagagttga
    20341 aagagcttga aataacttca attatgccta ttcgaaaaac gattgttaaa tcagcttttg
    20401 aagatgcgaa caactatatg aaaaatggcg tcttgttacg ccaagtcatc aatgttattg
    20461 atgaagttga tttcaatagc cctgaagatc gtcattcgtt taatgatatt tacgaaaaaa
    20521 ttcttaaaga tattcaaaat gctgggaact caggagaatt ttatacgcca cgtgcagcga
    20581 ctgattttat tgccgaagtt cttaacccaa aacttggaga atcaatggca gaccttgctt
    20641 gcggaacagg aggcttcttg acttcgactc tgaaccgttt aagtagtcaa cgtaaaacta
    20701 gtgaagatac caaaaaatat aatacagctg tttttggtat tgaaaagaaa gcatttcctc
    20761 atcttttagc agttacaaat ctgtttcttc acgaaattga tgaccctaaa attgttcatg
    20821 gaaatacttt ggagaaaaat gttcgtgaat atacggatga tgaaaaattt gacattatta
    20881 tgatgaatcc accttttgga gggtcagaat tagaaacaat aaaaaataac tttccagcag
    20941 aattacggag ttctgaaaca gctgatttat ttatggctgt cattatgtat cgtttgaaag
    21001 aaaatggtcg tgttggagtt attttacctg atggttttct atttggtgaa ggtgtaaaaa
    21061 ctcgcttgaa acaaaaactg gtagatgagt tcaacttgca tacgattatt aggttgcctc
    21121 atagtgtctt tgcaccgtat acaggaatcc atacgaacat tcttttcttt gataaaacaa
    21181 agaaaacaga agaaacttgg ttttatcgtt tagatatgcc agatggttat aaaaatttct
    21241 cgaaaactaa gccgatgaag tcagaacact tcaattctgt tcgtgactgg tgggaaaatc
    21301 gtgaagagat tctggaaggt aagttctaca aatctaaatc atttacacct agtgaattgg
    21361 ctgagttgaa ttataattta gaccagtgtg gctttccaaa agaggaagag gaaatcttaa
    21421 atccctttga gttgattcag aattatcaag cggaaagagc aactttaaat cataagattg
    21481 ataatgtatt agctgatatt ttgcagttgt tggaggacaa ataatgacac cagaacaact
    21541 taaagcaagt attctccaaa gagcgatgga agggaaatta gtgccgcaaa atcccaatga
    21601 cgaacctgca agtgaattat taaagagaat taaagctgaa aaagaaaaac ttatcagtga
    21661 aggaaaaatc aaacgagata aaaaggaaac tgagatattt cgtggtgatg atgggaaaca
    21721 ttatgggaag tttgctgatg gaagcactca agaaattgat gttccttatg atattgctga
    21781 tacttgggag tgggtgaggt tttctacatt ggttgaaatt gtcagaggtg gctctccacg
    21841 accaatcaaa gattatctta cttctgaagt agatggaata aattggataa aaataggtga
    21901 tactgaaaag ggtgaaaagt atataaataa tgttaaagaa aaaatcaaaa aatcagggct
    21961 taacaaaact agatttgtaa aaaaaggtac atttttgtta actaattcta tgagttttgg
    22021 tagaccttat attttgaatg ttgatggtgc aatacacgat ggatggttgg ctatttcgaa
    22081 ctatgaaaac tcattaaata aagattacct attctatatt ctttcatcaa atgtagttta
    22141 ttctcaattt ctatctctaa ttagtggagc tgttgtgaaa aacttgaata gtgataaagt
    22201 tgcttctatt cttatccctc tccctccgct agctgaacaa caacgcataa tagaagcaat
    22261 cgaatcagct ttagaaaaag tagatgaata tgctgaaagt tataatagac tagaacagct
    22321 agataaaaaa tttccagata aactaaaaaa atctattctt caatatgcta tgcaaggaaa
    22381 a
//
LOCUS       DAVKJU010000031        22095 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVKJU010000031 DAVKJU010000000
VERSION     DAVKJU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22095
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.031"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            104..268
                     /locus_tag="VW191_001131"
     CDS             104..268
                     /locus_tag="VW191_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU9623907.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFAGRK"
     gene            352..918
                     /gene="amaP"
                     /locus_tag="VW191_001132"
     CDS             352..918
                     /gene="amaP"
                     /locus_tag="VW191_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEU9623908.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLQKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            930..1100
                     /locus_tag="VW191_001133"
     CDS             930..1100
                     /locus_tag="VW191_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEU9623909.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1139..1747
                     /locus_tag="VW191_001134"
     CDS             1139..1747
                     /locus_tag="VW191_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEU9623910.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1778..1981
                     /locus_tag="VW191_001135"
     CDS             1778..1981
                     /locus_tag="VW191_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEU9623911.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2091..2369)
                     /locus_tag="VW191_001136"
                     /pseudo
     CDS             complement(2091..2369)
                     /locus_tag="VW191_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2567..2854
                     /locus_tag="VW191_001137"
     CDS             2567..2854
                     /locus_tag="VW191_001137"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9623912.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANIGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3132..3902)
                     /locus_tag="VW191_001138"
     CDS             complement(3132..3902)
                     /locus_tag="VW191_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU9623913.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSILGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3917..4765)
                     /locus_tag="VW191_001139"
     CDS             complement(3917..4765)
                     /locus_tag="VW191_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEU9623914.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGLQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4762..6237)
                     /locus_tag="VW191_001140"
     CDS             complement(4762..6237)
                     /locus_tag="VW191_001140"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEU9623915.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKEMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTPTKTGDSQS"
     gene            complement(6560..7915)
                     /locus_tag="VW191_001141"
     CDS             complement(6560..7915)
                     /locus_tag="VW191_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9623916.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGTFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7925..8242)
                     /locus_tag="VW191_001142"
     CDS             complement(7925..8242)
                     /locus_tag="VW191_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9623917.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8259..8576)
                     /locus_tag="VW191_001143"
     CDS             complement(8259..8576)
                     /locus_tag="VW191_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9623918.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8591..9487)
                     /locus_tag="VW191_001144"
     CDS             complement(8591..9487)
                     /locus_tag="VW191_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEU9623919.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9490..10752)
                     /locus_tag="VW191_001145"
     CDS             complement(9490..10752)
                     /locus_tag="VW191_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9623920.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11032..12210
                     /locus_tag="VW191_001146"
     CDS             11032..12210
                     /locus_tag="VW191_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEU9623921.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLISQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(12438..12884)
                     /locus_tag="VW191_001147"
     CDS             complement(12438..12884)
                     /locus_tag="VW191_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623922.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGFIQKIRLEDSNLHIGRFFINPQKQEQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSILKLE"
     gene            12954..13613
                     /locus_tag="VW191_001148"
     CDS             12954..13613
                     /locus_tag="VW191_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU9623923.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWKLLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(13585..14361)
                     /locus_tag="VW191_001149"
     CDS             complement(13585..14361)
                     /locus_tag="VW191_001149"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU9623924.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIYFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(14354..15577)
                     /gene="trpB"
                     /locus_tag="VW191_001150"
     CDS             complement(14354..15577)
                     /gene="trpB"
                     /locus_tag="VW191_001150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU9623925.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(15555..16154)
                     /locus_tag="VW191_001151"
     CDS             complement(15555..16154)
                     /locus_tag="VW191_001151"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEU9623926.1"
                     /translation="MTKVKICGLSTEEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPSDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(16141..16908)
                     /gene="trpC"
                     /locus_tag="VW191_001152"
     CDS             complement(16141..16908)
                     /gene="trpC"
                     /locus_tag="VW191_001152"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU9623927.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLGAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVVQRIKELQIDKG"
     gene            complement(16905..17909)
                     /gene="trpD"
                     /locus_tag="VW191_001153"
     CDS             complement(16905..17909)
                     /gene="trpD"
                     /locus_tag="VW191_001153"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU9623928.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVVLARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(17920..18486)
                     /locus_tag="VW191_001154"
     CDS             complement(17920..18486)
                     /locus_tag="VW191_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU9623929.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(18483..19844)
                     /gene="trpE"
                     /locus_tag="VW191_001155"
     CDS             complement(18483..19844)
                     /gene="trpE"
                     /locus_tag="VW191_001155"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEU9623930.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFEFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(20390..20467)
                     /locus_tag="VW191_001156"
                     /pseudo
     CDS             complement(20390..20467)
                     /locus_tag="VW191_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(21075..22076)
                     /locus_tag="VW191_001157"
     CDS             complement(21075..22076)
                     /locus_tag="VW191_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9623931.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         6924 a         4783 c         3706 g         6682 t
ORIGIN      
        1 tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt gctggaggtc
       61 ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa tcatcgcaaa
      121 tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta gttggggtcc
      181 atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg tgattactgt
      241 agtgtcattc tttgcaggta gaaagtaaac ttttcgccag taaagttagc aaactatttt
      301 taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta tatgtcaaaa
      361 gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt ccttcctgtt
      421 ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg gagacagaac
      481 tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt tctatcaact
      541 ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt ggaaatccaa
      601 cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg ttttgttaga
      661 agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa tttacaaaaa
      721 aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat cgctgacaga
      781 tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg tattgagcgt
      841 caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa caaaaagact
      901 gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat cgatatccaa
      961 ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt ggcttcttca
     1021 aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta tatatcgaaa
     1081 aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg agtttcatat
     1141 gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg ttgtagctca
     1201 cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca ttggtctttc
     1261 actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa atcttaaaga
     1321 aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg gtaaaacaca
     1381 agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag ctttatattc
     1441 agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg aaattgttga
     1501 aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag actcagtaag
     1561 ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt cagaacaatt
     1621 cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa aagttagcga
     1681 aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa acactcgtgt
     1741 aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag aaaaattaaa
     1801 tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg aaaaaatgga
     1861 aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagttgccg aagacgctaa
     1921 agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg acgataaata
     1981 aggttaaaag ttactttatc tttttggtaa cattagtcaa aagagtctga gtcaagatga
     2041 ttctcagaaa acaaaaagct agagattacc aattgcggaa ctctagcttt ttaattttgc
     2101 ctctttctct tattatattt cagcaggttg ttggccatga gtatgaatcc catgtcaatt
     2161 ctcacttgac gcttacctct cagatgacat ctcttataac ccaaacaaac ctttatctgc
     2221 ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc cttggaagat
     2281 aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat atacagtccc
     2341 ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag gctgtttgct
     2401 ttctatctcc cctttttcat gatatatttt cgtacaggtg cttcaatagt ttctatagta
     2461 aaatgaacca aaaatagtac acgttgcggt ataatgttct tgtggcatat tcattagatt
     2521 ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat agtcaattga ttgtcagttt
     2581 gaagaccaag agtcgtaagg caaaggatat ggcagaatcc atccaagggt ggctggcaca
     2641 atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt tttcaaaatg
     2701 gaaagatatt tccaatcatc acgatagtga gagctttttt gcgaatatag gatgtccaag
     2761 acagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact tgccaaagca
     2821 gacagatttc aacgaagtca gtcaagaatt ttaggctgga caaaaactca atttcaggag
     2881 aaaatgaaat aaatattccc acaataaaac gcatagtatc aaagtttttc caacacctga
     2941 tattatgcat tttttaattt taaagacttt ctcccagcct caagaaatca catcccacct
     3001 aaatcctttg gataccaaat acctcatcag gtattttctg agttggtaag actgtctagc
     3061 ttaatttgac aaatggagag cgttaaatcc agtttttaca ggaagtctat ttaaatattt
     3121 tcacactgac cttacaaaat atgaaataaa ttatataaag tcattcccat gacaaagaat
     3181 aacatccctt taaaacagta tcttacccaa ttttcaggta agacttttga tacaaccgtc
     3241 cccaataccc ctccgcatat agaagcaaga aaaataactg gaacaggtgc taaatgatac
     3301 tgatttaaac ctgtaaccac aacaattgta gccaaatgag acagctggga aaagaaaata
     3361 attgcaagag aatacatcgt tgcttctttt atcgaaatag aaaaaaacac catcaataaa
     3421 gatacattga gcggaccacc accaatccct aaaaaactag aaattaagcc caatagaaga
     3481 ccacaaatca tatagaatcc tatcttatca aattgataag tttttttaaa atctgttaaa
     3541 acaaatgata caagtagcat cacaaatagt aaaatcattt gaacaatgga tactagccgt
     3601 actgataact gagataacaa aacttgaaaa ataagatgac ctagaaatcc tcctaaaata
     3661 gaagaaaagg ataccgtaaa aatcaatctc cattttatct ctttactttg tgaaaatctt
     3721 ttgattgttg aaataatcgc cattataaaa acagagaaac tagaataaaa agcaatatca
     3781 ctgaccgaat ggtatccaaa actatccata agtggcttaa ttataattcc accacccatg
     3841 cctgatatag aacccaatcc actagctatt ataatgatta gaaaataaat aatctcaacc
     3901 actttttaag tccatcctat tctttcaaac atctaaaacc tagatggcta ctagtggaag
     3961 tagaaataca tccatttcta gcagctactc ggtatcgatt acaataagaa caatgacata
     4021 aaaaggaacc tcctctgata gcatactcct catcttgtat cccatatttt ggtaactcat
     4081 agtcatcttc attaaaacta gctaataagg tatatctcgg attgcgacac cattcccaaa
     4141 cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag acaggagccg
     4201 ttcctataaa tccatctaag gcagtatttt cataaggaaa tcttccttgc caagtattgg
     4261 catgataata tccaccttct aaaagagtat ctccccaagg atattcagat tgtcttcctc
     4321 ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc caattacaga
     4381 aagccaatgc atcttctaaa gcaacatgaa ctactggatg gtcttctaaa cccactagat
     4441 tactgttctc accgtaggga tgtttccagc aagcgttcga aacttgtaac caccacggag
     4501 ccccagcagg atgaggatat ccctcccttt ctgcctccgg aacgaacaat ataaaaacaa
     4561 aagaccactc ttgtcgctca gctagtgttt tatagcctgt ttctttcaca aattgagcaa
     4621 attcttgatt cgtaactgga gtatccgcaa tggaaaaatc ctttacacta acagcgactt
     4681 gaagaccttc aaaatcatct aaaaagccct cttcagaatt tgttcccatc ataaatgtcc
     4741 cacccttgat ttttttcatt ttcatgattg actatccccc gttttcgttg gagttggcgt
     4801 tattttagaa agttcaacag gaactaactc ttcatccaca acaaaacctt cttctctata
     4861 tctaagaaag tctactaatt ttttcttcat ctgacgaaca ataggttgat atttttcact
     4921 tggatacaaa tcattcattt catgcggatc ttttttcata tcaaataact gataatgatt
     4981 taaaacagga aaccagataa atttccactg gtctgtcaaa atatattgac tagaatcttt
     5041 tcctaaagca tgttcaccat gaaattctgt tcgccacccc tcgtactgtc caaataataa
     5101 attcttaaca ctccgtccat ctagttcatc tgtagtagtt cctcctgcca aatccacgag
     5161 tgaaggaaaa atatcttgaa ttttcactaa ttgcttgatt gttcccctat taccagctat
     5221 caatccagcc ggatcgtaaa taaacgaagg aatgtggata cttccctgat aaggatatcc
     5281 ctttctaaac agataatgtt caccaagctg atctccatga tccgaaacaa accagataat
     5341 cgtatcttta tcatgacgaa attcttttag ggcagtcaaa aagcgactaa tttgatggtc
     5401 tatatgagta atcaatccaa aataagcagc aaccattctt ctttgatcat cttcctttaa
     5461 tttccctctt aaagcataaa tagacggaat ttgcttttct aatacttccc aattcccgat
     5521 atgcaaatcg agaaattgag gcaagcgttc catatacata tcaaaatagt attttggggg
     5581 atttaatgga gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa ccgttggatc
     5641 tcttctttgt aaaaatgaaa tggactcact cacaacccag tttgttggat gtaacttctc
     5701 atctttatcc caaggtctag cttcccaaga attacaatcc attccgtcat caatcaaatc
     5761 aacatcataa ccaactttac cctttaaaaa ggctagatag tcactaacat aatcaaattg
     5821 acttccataa gccttatcat actttctatc aacatgtaga tacccatcgt gaagtaagac
     5881 atgatcaaac ccaagtctct gtctggaagg aaaaacatgc atcttaccaa tacactctgt
     5941 ttgataaccc atctctttaa agacttttgg aagagtatta gtaaaattcc aaggcacctc
     6001 atcttgataa ccaacacgtc cacttttatc ttggtctaag ccagtcaata aagctgccct
     6061 agcaggaacg caagaaggaa ctggagaata ggcattttca aaattataac ctacactagc
     6121 catcatatct aatgttggag tgctaactag cttatcctta ctattcaagg ataaggcgtc
     6181 tgctctcatt tgatctacaa caatcaaaat aatatttggc tgttttgtct gaaccataaa
     6241 atctcctttc taatatggca aaagaggcac aagaagatat ctacctttac tgcacccctt
     6301 tctatatcaa tctctctata taaagcaata acattcttgt tatgttttat agaacaatga
     6361 actaaaatat gactaaatcg attaggaaat tcaaatcatt ttctagtact gttttagaag
     6421 ttacagtgta ctattccaac ttcaataaat tataaacctt tgtctaataa caattttagt
     6481 ggagataaga aatcctacac ctaactcatc ttacacgtaa tctatttcta ttttatcaca
     6541 aaaaacgcaa gtaagaccat taactcaatt cagttttatc tgccattttc acaaatggga
     6601 aataagtcaa gacactaata atcaaacaaa caactgataa gatgatggca cgccaatcaa
     6661 atgctgtaga gaagaaacca tataaaattg gaggcattac ccaagtaaca ttttgtgtaa
     6721 caggtgaaac aagaccccag cttgttgccc agtaagctac cgttgccatg aaaaccgggc
     6781 taagtacaaa tggtataaat agcaaaggat tcaagacaac tggtaaacca taattgaccg
     6841 gctcaccaat attaaacaga actggtgcta gaccaagttt agcaactttt cgataatgac
     6901 tgtttcttga aaaaattaaa atagcaagta ctaatcctaa tcctccaaac cagacaaacg
     6961 tcccaaaaga cccacttgtc catatataag gaatcggttc acctttttgg aaagcatcca
     7021 gattcgctaa catagcaact ccaaatagcc cttccatgat gggagccaat acatttcctc
     7081 catggagacc aaaaaaccag aataacttat tcaaaaagat catcagaata actgcaaaga
     7141 aactttgaga caaacctagt aatggcgttt gtaacacctt gtaaacccaa tcaatcaata
     7201 agtcattgct aagtaaatgg aaaacataag tcaagatggc tactatatac atcgccataa
     7261 atcctggaat gatagaagtg aacggcttag caatcgcagg gggaactgaa tctggtaact
     7321 tgattaccca gttctttttc attactttac agaaaataat agaggctaaa aatccaatca
     7381 tcatggctgt aaagtagcct ctggcattaa tatggtttcc tggaatcaca ttcccaatag
     7441 ttaccatcag atttttacca tcaaatgcta gattatcaat tccttgtaag atctgatcta
     7501 atttcacatc tcctacattt gccaaaggga aactctttgt aactgtactt ccaatcgaaa
     7561 tgacaaacga agcaagtgat accaaaccag cagaaactgt atcaaccttg taaatcttag
     7621 cgatattcac tcccaagcaa tagatgaaca acaaggaaac aattggtata cttcccttga
     7681 ataccaaatt attgatgtca acaagccact gaaaggtttt cgtaatactt tctaggtgaa
     7741 attgttgtgg taaatccact agaaaagcat ttaataacaa agcaatggaa cctgtcataa
     7801 taacaggcat agtccccaca aatgaatcac gtaaagcaac taaaaacttc tgattcccaa
     7861 ctttcattgc aattggagaa agtttttctt gcattttatc caacattgta ttcatactaa
     7921 actcctaatt tttttcaaat cgttcgtata cttctaccac ttctttcgcc aagtcaacaa
     7981 aagtcagact cgtcattaaa tgatcttgcg cgtgtaccat caagaggcta attgatgttt
     8041 cttccccctg tgcctctctt gtcaacattt ctgtttgagc cttatgcgcc tgtaataagg
     8101 cagcattcgc atcagctaat cttcgattgg ctttagagaa atcccctttt tttgctgcct
     8161 gaatagcctc catagcatta cttttggctt ccccaccata cataattagc cccattgcag
     8221 attctaaatt actttcattc atagcattct cctcaatctt agtctaataa actttcagct
     8281 aaatctaaga ctttagaacc attcatcatt ccataatctg tcatcggaat aaccgccaca
     8341 ggaatctgtc tatcttttag tttttcttga aagtccccta gtaaatagcg aacttgaggg
     8401 cctaaaagca acacatttac ttcctttgtt gctacaattt cttctgcttc aggagctgga
     8461 actgcaaaaa tttctgcatc caacccctta tcttctgctg ccttttgcat ctttgtcact
     8521 agcagacttg tactcatacc tgctgcacat gctaacataa ttgttacttt agccatagct
     8581 tactccttta tcacctattt actgacctag ccaagcgaga tactgtatgt ctcactcctc
     8641 taattgcctt gctcaatgca taaatattat cgactgttgc cagtccccca tatcctgcgt
     8701 cacctatatg ttgaatatca actccacaaa ttttatttct aagtgcaatt tccttgatag
     8761 tatctgtatc agatgtttct tggctagtac caatagcact cagtactagc ccacccttac
     8821 tatgaacgag atcaacgact tcacgcaact cttggtcatg aaaggctgga acggttccaa
     8881 ctgctggaac aagtatcaca tccgcacctg cttccaacaa ttgctctgct acagaaagct
     8941 ctgccacagg ctcattcact cctgccccgt gcatcttacc tgcaataatc aaaccagaaa
     9001 agttttcctt agcagtttga acagccttaa tgatttctcg attgctaact cctgttccag
     9061 gatttccagt caaacagaca aagtcaaaac ctaactcctc tattcgattc aatgtttcaa
     9121 cactagcaac acgacctgca acaatttcct gtgtttcctc tagcatcttt gcagatgggt
     9181 caatcggttc caaatttaca ccaattggac aagcaacaag ctggtgtaag cgatgaataa
     9241 catcatcacc tgaactatcc aaagcataaa ttttaggctc aaaaacatca acacaattca
     9301 ataaaatcat atcagctcca aaagctctag caatttcaga attagttata tccccaacaa
     9361 atgtctcacg agtaactaca ttttcagata gaacaactct accttcactt gctaaaatac
     9421 tttgttttaa ttcttcagca ttcatctgta atatctcaga tggatttgca ctaattaaac
     9481 gtttcattct caatcctcca ataacttaaa ccgtttttca atagctatta caaatggtaa
     9541 ataaagtaag acatccaaaa caatcaatac aatttgtaat aatgaaccag aaatacttcc
     9601 tgtagcaagt aaaccactta taacaggtgg catagtccaa cctggatctg tataagttag
     9661 cggaaccaaa cctgatgcca ttgctcccca cgctactagt aagttaaaca ttggagctaa
     9721 gataaatgga actagtaaca aaatgttcaa aacaatagga acgccaaaca ttgctggttc
     9781 atttatatta aataatccag gtataacagt tataggtgcc aaagttttta gttgtttact
     9841 agctttcttc ttatgtgcta aatatcctaa cgcaagaact aaacctatgg ttgcaccacc
     9901 gccacctatg aatacaaaat tatccataaa tacgctagta aaaatatttg gaagagtttc
     9961 tccagtttga taagctactt tatttgcatc tagattagct aaccataacg gtttcataac
    10021 tgcattaaca acatttcctc cgtgtaatcc tacaaaccaa aaactactat taaggaaaat
    10081 taagataaga agaccaataa cattatttcc taaaagtcca agtggacctc ccaaagctac
    10141 ttgagcaatt tcatgaagat taggtaaact taatttatct aaagttgcat aaacaatcaa
    10201 ccaaccaaca atagtcactg caccaggaat aatggcacta aagcttttag atactgctgg
    10261 tggaacacca tccggcattt ttatctgtat attatgattt ataaaccatt ggtagatata
    10321 gccattaatc aatcctaaaa tcatagcaac aaataaacct tttgatgcca tgaaagcggt
    10381 aggcattcct gctccagcct ctcctctaat gaaaggcgta actgtaataa aagatgataa
    10441 tgacacaata ccagcaggaa taccatcaac tttgtattgt ctagccataa agtaagcgat
    10501 accaaaacta gctacaagac caagtaatcc aaaactacta tcaacaccct tccataaata
    10561 gtcagcaaca ccaatatcac ccagatattt ttcccatcct ggaataggaa aacttgcaat
    10621 aaccattagt aatgaaccta tcaacaataa aggtatagtt agagtaatac cgtctcgaat
    10681 agaaaccaac gctttattat tacccaatct agccgctact ggaagtaact tttcttgtaa
    10741 taatttttcc ataacctttt cctcttaaat atattttatt atcaaaagtt atcaattatc
    10801 tttatttttt gacgtcaagt tgttatttga ttatcttttg ttatttatat tttacaacta
    10861 taagaaagcc ttgtcaagga tttgtgtaaa tatttgatta ttttttatta tcaatatata
    10921 ataaaaagag accagtaagc aatgaaaagc ttaaaaagat taacatctac atttatccat
    10981 agcaaaaaat cataggattt taagaatctg acaactagga agaaaactaa tatgacctta
    11041 tctaagaaac aattacaact acgagctaaa atactggaaa cagtttacac attaggtccc
    11101 atttcacgaa ttgaaatcgc tactaaaaca ggaataacac ctgcaacaac tagcagtatc
    11161 acaaatgatc tgattaaaga aaacattctc ttagaacttg gtgaagacga acacgataca
    11221 agcgtaggga gaaaaaaaat cctgctagac attcaagcta aacgatttta ctacattggt
    11281 tgtgaactat ctgaaaaaca tttcactttt gctttaggag ataatctagg aaatatctta
    11341 aaggaagaga aagaaattgt tactaaacag ttaatccaag aaaagggaaa tcaactcatt
    11401 agccagacac taaagcaatt cttaaataac tgctctgatt atgaaataga agcaattgga
    11461 atcgcgctac ctggtcgcta tttagatgat tataaaataa caacaaacaa tcctttatgg
    11521 caacatatcg acttagaaat gattcaatcc cactttgata agcctttgtt tttctctaac
    11581 aatgttaact gtatggctat aggtaaacgc ctatttagtc gccaacagaa tgaccctaat
    11641 tttgcttatt ttcattttgc tagaggtatg cactgttctt atatctacga cggaaacatt
    11701 tatggaaaag gaaatctaat gattggagaa atagggcata cagttgtttc atctgaggga
    11761 gaagaatgta gttgcgggcg caagggatgc ttacaaactt ttgctggaga atcttggtta
    11821 atcaaaaaat caaaaattct atatcatcaa tctccctatt ctctacttcc tagtttagtc
    11881 aaaaatgccg atgatattga tatccaagtt attttaaccg cttatcaatt aggtgacact
    11941 ggaataatca cacttatcca tcaagcctta ctctatctta gtcaaaccat tttaaatatc
    12001 agtatgatga ttgactctca aaaaatctac ctacatagtc ctttactaac aaatcaacac
    12061 atcatccaaa aactatatag tgaaatgaac tacaaaccca aactgctcta taatcgccta
    12121 cctgaagtca ttattgaacc ttacaatgat tttacagccg cacactcagc tattgcactt
    12181 tgtttgtatc atacaattct acattcctaa ttttctatta catttaataa ataggctaaa
    12241 aagagggagt atatcataaa aagcgaacaa acctgttttc tgaaaatcag aattctgttt
    12301 tgttcgcttt ctttatattt ggaattatac tcctatctcc cctttttcat aatgtatagt
    12361 aaaatgaaat aataacagga cgaatcgatc gggacagtca aatcgatttc taacaatgtt
    12421 ttagaagtag aggtgtacta ttctagtttc aatatactat attttcgtac aggtgcttca
    12481 accatttgaa cgatttcaaa tccttctttt tggtaaagat tctgagctct ttgatttgcc
    12541 tcgaagacat ttagagaaat actatctata tcttcatttt caaaggccaa actaacaaat
    12601 ttccttaaag cctggctacc taagccttgc tcctgtttct gggggttgat aaaaaatctc
    12661 ccgatatgaa gattgctgtc ttctagcctg attttctgga taaatcccac aaactcttgt
    12721 tcatcaaaga ttgaaaagac tccttccaag gcttgaaggg tcagtagaaa aggaatcctt
    12781 ggtcccatcc attgttcttg aaaggatttg cctagggagt tggaccactg gcatacaaat
    12841 tgagcgtttt ctatactcac cttttcttca aaacgaattg tcatcttttc ctcaccatct
    12901 tatctatgtt tctccattat actatttctc ccatttttta cgaatagata agtatgattg
    12961 atttttattt ttttctcgtc gggagcattc tcgcttcctt tcttggtttg gtcattgacc
    13021 gttttccaga gcaatccatt atcagttcag ccagtcactg cgattcctgt cagactcgct
    13081 tgcgtccctt agatttgatt ccgattctct cacaggtctt caatcgcttt cgctgtcgct
    13141 actgcaaagt tcgctatcct gtctggtatg ccctctttga attagtctta ggactcctct
    13201 ttctgcttta ctcttggaaa ttgctttcct tggggcaagt agtcctaatc accgctggtt
    13261 tgaccttggg tatctacgac tttcaccatc aggaatatcc cttactggtc tggatgactt
    13321 tccacctaat cctaatagct tcctctggct ggaatctggt catggtctcc ttcctcatac
    13381 ttggaatttt ggctcatttt atcgatatcc gcatgggcgc aggggatttc ctctttttag
    13441 cttcttgtgc tctcgtcttt agcgtaacgg agttactgat cttgattcag tttgcttctg
    13501 cgacgggtat cctggccttt ctcctgcaaa agaaaaagga aagacttcct ttcgtgcctt
    13561 tcctcttact tgctacttgt ttgattattt ttggtaagct actgcttgtt tgataaagtc
    13621 ctgaatcggc tctccttggt ggagagcttt tactattttc gaaccgacga taacaccatc
    13681 tgacaccgca ttgaagcgtt ccagatcggc ttgactagat acaccaaaac ctgtcaagac
    13741 tgggatgtcg gccacttgat gaagttgcgc caagtgcttg tccaaatctg cacggtaatt
    13801 gcctgatttc cctgtcactc cattgatggc aacggcatag atgaatccct ccgccccttc
    13861 aatcaactct ttctggcgct caattcctgt ggtcaagctt actaaaggaa tcaaggcgat
    13921 atctgtatct gccaaaaatg gttctacaaa gttggcatgt tcatgaggca agtctgggat
    13981 aatcaagccc ttcacagctg tatcagccag atctttgaca aagttctcca caccgtactg
    14041 aaagaggggg ttgaagtagg tcatgatgac cagtggaatc tctgtttcaa tggttttcaa
    14101 ggtttcaact aaagcctggg tagaggtccc gtgggctaaa ctgcgcaagc cagcttcttc
    14161 gataacaggt ccatctgcaa cagggtctga aaagggaata cccacttcaa ttgcagagac
    14221 acccaaatct tctaaaaagt aaattgtttc agcaagaccg tccaaacctt tctcgtggtc
    14281 accagccatg atatagggaa caaaaattcc ttttccagct gctttaatag cattcaattt
    14341 ttctgttagt gtcttaggca tgagcttctc ccttctttgc tgcatctgct tccaagcggt
    14401 ctttgacttg aaccacatcc ttgtccccac gacctgatag gcagacaatc atagactttt
    14461 ctggtccaag ttctttggcc aatttcaccg caaaagcgat agcatggcta gattccaagg
    14521 ctgggataat cccttccaca cgagacaaga gttggaatcc ttccaaggct tcttcgtctg
    14581 tcacagggac atagctggca cgtttgatat cgtggtagtg agaatgttct ggaccaatac
    14641 caggatagtc caaacctgct gagatagaga aggcttcaag aatttgacca tgggcatctt
    14701 ggagcacatc catgagagaa ccgtgaagaa cacccggacg acccttggtc aaggtagcag
    14761 cgtggtgctc tgtatccaca ccaagccctg ctgcttcagt tccatacata gctactgact
    14821 catcttctac aaagggatgg aagagcccaa tagcattaga tccaccacca acacaggcta
    14881 ctagagcatc gggcagattt tgacctgtca tatcgcgata ctgttgttta gcttcgcgac
    14941 cgatgacact ttggaagtca cgaacgattt ctggaaatgg atgaggcccc aaggcagaac
    15001 caaggatata gtgggtatcg tcgatattag ccacccatga acgaagggct gcattgaccg
    15061 catccttgag cacgcgcgaa ccatctgtta cagcctcgac cttggctccc aaaagctcca
    15121 tgcggaagac attgagggct tggcgtttga catcttcctc acccatgtag atggtacatt
    15181 ccatgttaaa gagggccgca gcagttgcag ttgccacacc gtgctgacca gcaccggttt
    15241 ctgcgataat tttcttttta cccatgcgtt tggcaagcca aacttgtcct aaggcattgt
    15301 taatcttgtg ggctcctgta tggttaaggt cttcccgttt gagataaatc ttggctccgc
    15361 caatatgctg ggtcaagttt tttgcgtagt aaagaggagt ttcccgcccc acatactggc
    15421 gcaagagttg gtttaattcc tcttggaaac ttgggtctgc ctgactttca cgataggcct
    15481 tctccaactc caaaactgct gtcatcaatg tttctgggac aaaacgtccg ccgaattttc
    15541 cgtaaaatcc atctttattt ggttcctgat atgccatgct ttaccctctc tataaatctt
    15601 ctaatctttt catgatcttt ttgtccatct gtctccactc cgctcgatac atctactgca
    15661 tagggagtaa aatgttgaat tgcttttact acattatctt cattaaggcc acctgcgata
    15721 aagaagggct gtgctagtcc agtcgtatcc agttgacccc aatcaaaggg ctggccactt
    15781 cctgccacag gggcatcaaa gagtagataa tctgcctgag aattggggac atgcccattt
    15841 ccatctacct gcacagcctg aatactggca caaggcaaat tctcaaataa atcatctgcc
    15901 acctgaccgt gaacttgaac caagtccaag ccaactttgt caatcgcttc cagcagttct
    15961 acccgacttg gtgaaacaaa tactccaacc tttttcacat ctgaaggaat aagctttgcc
    16021 aactcagctg cctcttctaa agtcacctgt cttttactag gtgcaaagac aaaaccgatg
    16081 tagtcggctc ctgctgaaac ggctgtttcc accgcttctt cggtcgatag tccacaaatt
    16141 ttaacctttg tcaatctgca actccttgat tctctggacc acattttctg cctgcataag
    16201 agctgtccct accaaaattc cgttaaagta tggggctagt cgttccgcat cctgccctgt
    16261 gaaaatagca gattcagaaa tgtacaagca atcatctttg aaatgtttag ccaagtctac
    16321 actggtctgc aagtcgacct caaaggtggt caagttgcgg ttattgaccc cgataatctg
    16381 ggcaccaagt ctgtgagcca cttctagttc agctagattg tgagtttcca ccaagacttc
    16441 cagaccaagc actgtcgcgt agtcgtaaag ttccttaaga cgttcttctg ccaaggctgc
    16501 cacaatgagc aagatgactg tcgcacctgc attgcgagcg cggatgattt gcttttcatc
    16561 gatgataaag tccttgttga gcgtcggaat ctctacctga ctggaaatct cccgtagata
    16621 atccaaatgt cctttaaaga aaacttcatc tgtcaacacc gaaatcatca ctgctccgtt
    16681 ttcttcataa gtctgggcct gttgcacaat atccacatcg agattgatat ctcccaaact
    16741 agggctagct ttcttgacct cagcgattac ctgcaagcgg tcctgatgat tcttcaaaaa
    16801 ttctgccaag cgataggtct ggcgcagagg ctggatttgc tccagcttca tctgctccac
    16861 ctcacgcgcc ttctgctcta agattcgtgc taaaaattcc tgactcattt ttggtactcc
    16921 tgtaacagtc tgagtttttc aagggccttg cctctagcaa tcacttgacg ggccaagaca
    16981 actccttcct tgatgctatc aatcttacca ttagcataga aaccaagacc agcattcaag
    17041 actgtcgttt ccaagaatgg acttgcttcg tttttcagaa cgctaagcaa aatttctgca
    17101 ttttcctgag cattcccacc acgaatatct tccatagcat agccttccat tcccaaatcc
    17161 tctggagtaa agcttgacaa gctgatttcg ccattttcaa gaagtgcaat cttggttgtt
    17221 ccgttcaagc cagcttcatc caacccttct ggtccagcaa ccacgatggc acgtttgcga
    17281 cccatatttt tcaaaacctg agctgtactt tctaggagtt ctggacgact aattccaaga
    17341 agctgtgttt ctaaagccat tggatgaatc agtggaccag tcaagttcat aatcgttgga
    17401 attcccaatt ccaaacgagc tggcatgatg tatttcatag ctgggtgcat atttttagcg
    17461 aagagaaaga cgattccagt tttatcaaag accttaccta gttcagctgg tttgagatca
    17521 agattgattc ccaaggcttc gaggacatct gcggaaccag atttagaaga aatcgagcgg
    17581 ttaccatgct tggccatgtg aataccgcca ccagccaaga caaaggctgc agttgtggaa
    17641 atattaaaac tgaaagactt gtccccacct gtaccacagt tatccatggc atcatgaatc
    17701 tcagttggaa tatgctgggc atgtcctctc atgacttggg caatggctgt gcgttcttca
    17761 ggtgtttccc ccttcatctt gagagctaag aggagagaag caatctgcgc ttcagttaca
    17821 cgcccagtta cgatacgctc aatgacatcc gtcatttcca cacctgataa attttcaaat
    17881 tttgctagtt tttcaataat ctctttcatc ctagtttcct cactttacaa ccttctcgat
    17941 aaaattccga atagaagaca agccgtctgg cgttccaatg ctctctggat ggtactggaa
    18001 gccataaatc ggtaggtttt tatgttgaat ccccatgatg gcttggtcat cagtcgaacg
    18061 agctgtcact tcaaagtctt ctggcatttc ctcaatcaaa atactgtgat aacgcatgac
    18121 cgcacgtcca tcctcaatac cttgatacaa aacagatggc gcttcaaagt tgatattgct
    18181 ctgtttccca tgcatgactt ttggagccaa acctagctta ccaccaaaga cttctgcaat
    18241 ggcttggtga cccaaacaaa tccctagaat cggcttcttg cctgcaaaat cacaaatcat
    18301 gtcttccatc tttccagcat caactggcca accaggacca ggagaaaaga ccagaccatc
    18361 tgctttttca gcttcttcat atagcttgga atcatcattt ctcagaacct gaacttctgc
    18421 aaaattccca atgtattggg ccaagttata ggtaaaagaa tcatagttgt caatcaataa
    18481 aatcatggtc ttagttctcc aattctagtc atagattttg ctttgttaat ggtttcttgg
    18541 tattcgtttt gggcgataga gtcgtagaca atccctgccc cagcctgcac ataggctctt
    18601 tgatttttga gaatcatggt tcggatggca atggccaaat ccatatcacc cgtcgcagac
    18661 aagtagccga ttgctcctgc atatacgccc cgtttttccg tttccagttc atagatgcgt
    18721 ctcatagctc gaatttttgg tgctccagaa acggttccag caggaagcgt tgctttcaag
    18781 gcatccatgg cagtgagttc tgcaagcaaa cgtcccttga ccacactggt caaatgcatg
    18841 acgtagcgga agagctccac ctccatatac ttagtaactt ggacactggc cgtttcagag
    18901 atgcggccaa tatcgttacg ccccaagtct accaacattc gatgttctgc tgtttccttc
    18961 tcatcagaga ggaggtcagt cgccaaggcc ttgtcttctt catccgtagc ccctcttggt
    19021 cgcgtccctg caatcggatt ggttgtcacg atgccatttt tgacagaaac caaactttct
    19081 ggactggctc cgatgatttg ataatcccca aaatcataga aataaaggta attagaagga
    19141 ttagtcacgc ggagatttct gtagaattca aatggatttc cagtaacttc tgctgagaaa
    19201 cgctggctga gtacacattg gaacatatca ccattacgaa tcaagtcgcg agcagtttct
    19261 accattacct caaacttatg tggagcgata tgcggtttga agtcaagtgg tgataaatcc
    19321 aagtcttcaa attcatttgg agcaggaatg cgtaattcct caagcacttg gttcaaggat
    19381 ttttccaagg cctcttgact gcgctcacta taaagtgcat cctctatgac atgtatcttc
    19441 tccttcttgt ggtcaaagac tatatagctc tcatagacaa agaaatgcat gtctggcgtc
    19501 ccaattgtat cctcagggat ttgaccaatt tcttcataaa gcgaaatcat atcgtaaccc
    19561 acaaaaccaa tggctccacc accaaaaggt agctctgaat ggtgctgact cttatgaatc
    19621 acttcataaa ggaaatccaa gggatcccga tcaatcactt gaccattttg ataaagaact
    19681 ccattttcaa acttaatctc aaaaacagga ttataggcta ggatagaaaa acgagctgtt
    19741 tccttgtctc tcggaatact ctctaagata accttgtgtt gcccctttaa gcgcatataa
    19801 gccaagattg gtgataagac atctccatga atgattcgtt ccattgtaat ttccctttca
    19861 gttctaattc tagttcgtag cgactgtatg aaaaatcccc acgcaaaata acttgcgtga
    19921 ggacgaaatt cgcggtgcca cctcaattat aggatttctc ctatctctca ttcctgtctc
    19981 agatatctcc tgtaacaggc tgtgcgataa agggcactcc cttgagaatg atgttttctt
    20041 ctctcgtttc agatgaaccc aactttacag ctttctctgc ttgttttcag caaccacaag
    20101 ctctctgtga gagaaagaac tgtaattttt ccatctatta ttttttagct tctagtagtc
    20161 tgcaatcgca gctaggtcct tgcctccacg accagagaca ttgatgaaga gatgttcatc
    20221 tcggtacacc tttatactct tcgaaaatct cttcaaaccg cgtcaacgtc gccttgccgt
    20281 aggtatggtt actgacttcg tcagttctat ctgcaacctc aaaacagtgt tttgagctga
    20341 cttcgtcagt tctatccaca acctcaaaac agtattttga gctgacttcg tcagttctat
    20401 ccacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt tgctctttga
    20461 ttttcattga gtattactag cttttttcgt attagtccag cctttttgtt tgcttttagt
    20521 agtaggcaag gagctgtaga tagaactcaa gttcatcaaa gcgacttaag gccctaataa
    20581 aagataaacc aaacgacgga tagaaaaaag cccacacaca gaatatactt ccgtgtgagg
    20641 gcgttggtaa cgcggtgcca cctcaattat aaagggacta tccctttaca tctctgcctt
    20701 gtttaacaac aagctgcact gtaaggtgtg cgcaccgaat tttcattgtt tcaaattcat
    20761 tttcaaaatc agcccacttt cactacttcc aaccacctat tcacaatcac cacaggctcc
    20821 ctgaagatca aaaatagtta cttttctgat ttgttgaact tattttaata ctttgttttt
    20881 tctttgtcaa gactttttta cgattttttt gaaaatatca ttcgaatatg accatgtctt
    20941 ccttagatcg aacatgaaca tgtcccactt gttagaaatt ggatccaact caatagaaac
    21001 tgaatggagg ctaaacagaa cttattttag aacactccat cttttccact aggattttca
    21061 agaattaaac aatactagaa actctgtctc ctaacaaatt taggagaaac ttcaacagat
    21121 gtgacacttt cccctttaat aattgctaaa acaccttcta tcatttcttt agccaattta
    21181 acataattgg gagcaattgt agacaaagct ggagtataat actgagaaat aggaatatta
    21241 tcaaatccaa tgatagaaat atcatctgga ataagaattc ctttctcata gcacgcacga
    21301 atcaagccct gaaccttttc atctcctgaa acaaaaataa tgtccggata attttgggta
    21361 gtcaagtgct gcattgcata agaataaact gaatcaattg tagataagcc ataaatgact
    21421 tttaaatcca taaagtaatt tttatcattc agaaaagaac gcacacctct ttcacgatcc
    21481 ttattaacat gggattctcc tcccataagc aaccacatat ttttaaattt ttcttcagtt
    21541 acagctttca tcatatcata agtagcttga aaattattat tagatacata gactactcca
    21601 gacgtttgag attcaccgaa aacaagaaaa ggcatatggt tcttctttaa atactgaatt
    21661 ctgatatcat ctacactttc ataaaaaaca ataacaccat ctactaggct acctgtgctt
    21721 gatataattg aattactaat tgtatcctcc tctccaaagt actcaactat agcattaaca
    21781 ccaaattctt tacacgtccg taacacttta tctaacagcg tatgaaacca aattaaagga
    21841 aaagagtcga ttttttttac agaaatcaat atatttatag cttctttttt agttaaattt
    21901 tttgcatacg catttggaat atacgacaat tcctctataa ctttttgaat cgcttgataa
    21961 gtttcttctt taacatttac tccaccatta ataactcgtg aaactgtttt tggagaaaaa
    22021 cctgataaac gtgcaatatc ataaatagtt acctttttcc catttatatt tttcatttca
    22081 gtcctccatt acgaa
//
LOCUS       DAVKJU010000032        22080 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVKJU010000032 DAVKJU010000000
VERSION     DAVKJU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22080
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.032"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..272)
                     /locus_tag="VW191_001158"
     CDS             complement(<1..272)
                     /locus_tag="VW191_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9623932.1"
                     /translation="MKSTKEEIQTIKTLLKDSRTAKYHKRLQIVLFRLMGKSYKEIIE
                     LLDCNQTTIWRNVKKYEEFGLDSLLQETRGGRNHAYMTVEEEKAFLA"
     gene            complement(656..1852)
                     /gene="tuf"
                     /locus_tag="VW191_001159"
     CDS             complement(656..1852)
                     /gene="tuf"
                     /locus_tag="VW191_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU9623933.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2763..3632)
                     /locus_tag="VW191_001160"
     CDS             complement(2763..3632)
                     /locus_tag="VW191_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9623934.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3855..4376)
                     /locus_tag="VW191_001161"
     CDS             complement(3855..4376)
                     /locus_tag="VW191_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEU9623935.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDKK"
     gene            complement(4334..5440)
                     /locus_tag="VW191_001162"
     CDS             complement(4334..5440)
                     /locus_tag="VW191_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEU9623936.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGRQEKLVESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5551..5805)
                     /locus_tag="VW191_001163"
     CDS             complement(5551..5805)
                     /locus_tag="VW191_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623937.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(6163..6609)
                     /locus_tag="VW191_001164"
     CDS             complement(6163..6609)
                     /locus_tag="VW191_001164"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9623938.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6682..7007)
                     /locus_tag="VW191_001165"
                     /pseudo
     CDS             complement(6682..7007)
                     /locus_tag="VW191_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            7241..8959
                     /locus_tag="VW191_001166"
     CDS             7241..8959
                     /locus_tag="VW191_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEU9623939.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            9070..9417
                     /locus_tag="VW191_001167"
     CDS             9070..9417
                     /locus_tag="VW191_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU9623940.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(9696..10532)
                     /locus_tag="VW191_001168"
     CDS             complement(9696..10532)
                     /locus_tag="VW191_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623941.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(10545..11174)
                     /locus_tag="VW191_001169"
     CDS             complement(10545..11174)
                     /locus_tag="VW191_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9623942.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(11184..11825)
                     /locus_tag="VW191_001170"
     CDS             complement(11184..11825)
                     /locus_tag="VW191_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9623943.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(11971..13200)
                     /locus_tag="VW191_001171"
     CDS             complement(11971..13200)
                     /locus_tag="VW191_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU9623944.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILELQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEYYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(13190..14356)
                     /locus_tag="VW191_001172"
     CDS             complement(13190..14356)
                     /locus_tag="VW191_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU9623945.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            14500..15513
                     /locus_tag="VW191_001173"
     CDS             14500..15513
                     /locus_tag="VW191_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEU9623946.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(15558..15977)
                     /locus_tag="VW191_001174"
     CDS             complement(15558..15977)
                     /locus_tag="VW191_001174"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEU9623947.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(15988..17394)
                     /gene="atpD"
                     /locus_tag="VW191_001175"
     CDS             complement(15988..17394)
                     /gene="atpD"
                     /locus_tag="VW191_001175"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU9623948.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(17480..18358)
                     /locus_tag="VW191_001176"
     CDS             complement(17480..18358)
                     /locus_tag="VW191_001176"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU9623949.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLSDQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(18374..19879)
                     /locus_tag="VW191_001177"
     CDS             complement(18374..19879)
                     /locus_tag="VW191_001177"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU9623950.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(19894..20430)
                     /locus_tag="VW191_001178"
     CDS             complement(19894..20430)
                     /locus_tag="VW191_001178"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU9623951.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(20430..20924)
                     /gene="atpF"
                     /locus_tag="VW191_001179"
     CDS             complement(20430..20924)
                     /gene="atpF"
                     /locus_tag="VW191_001179"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEU9623952.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(20938..21654)
                     /gene="atpB"
                     /locus_tag="VW191_001180"
     CDS             complement(20938..21654)
                     /gene="atpB"
                     /locus_tag="VW191_001180"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU9623953.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(21689..21889)
                     /locus_tag="VW191_001181"
     CDS             complement(21689..21889)
                     /locus_tag="VW191_001181"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU9623954.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(21964..>22080)
                     /locus_tag="VW191_001182"
     CDS             complement(21964..>22080)
                     /locus_tag="VW191_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9623955.1"
                     /translation="NNYPKKYLDYKSPREDFWMANLNLKFSKMEIIFIKRFQ"
BASE COUNT         6591 a         4743 c         4008 g         6738 t
ORIGIN      
        1 gcaagaaagg ctttctcttc ctcaactgtc atatatgcat ggttacgacc accacgtgtt
       61 tcttgaagga gagagtcgag tccgaactcc tcatattttt ttacatttcg ccaaatcgtt
      121 gtttgattac agtctaaaag ctctataatc tctttataag atttgcccat cagacgaaat
      181 agaacgattt gaaggcgttt atgatattta gctgtacgag agtcttttaa aagtgttttg
      241 atggtttgga tttcttcttt agttgatttc atattactat tgtataatgc tttttgattt
      301 gagtctagta ttatactctt cgaaaatctc ttcaaaccgc gtcagcttcg ccttgtcgta
      361 tagatgttac tgacttcgtc agttctatct acaacctaaa aacagtgttt tgagcagtct
      421 gcagctaact ttctataaca aaatgaaata agaacaagac aaatcaatca agataatcaa
      481 atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca atatattata
      541 gtttgctctt tgattttcat tgagtattag ttcaatatct aaaaaaagaa ccttgccaag
      601 caagattctt ttagtgtctg acttaaataa ttgttcttct gggaactaaa tcgaattaag
      661 cttcgatttc tgtaaccata cctgaaccaa cagtacgtcc accctcacgg atagagaatg
      721 tagtaccttg ttctacggcg attgggtgaa tcaactcaac gtcgattgtc acgttatcac
      781 caggcattac catttcagta cctgctggaa gttcgattga acctgtaacg tcagtagtac
      841 ggaagtagaa ttgtggacgg tagttgttga agaatggagt gtgacgtcca ccttcttctt
      901 tagtaaggat gtagacttca cctttgaatt tagtgtgtgg gttgattgaa cctggtttag
      961 cgataacttg tccacgttcg atttcatcac gttgaacacc acggagaagg acacctacgt
     1021 tatctccagc aagaccttcg tcaagttgtt tacggaacat ttcaacacca gtaacaactg
     1081 ctttttgagt ttcttctttg ataccaacaa tttcgatttc gtcgttgact ttaacgatac
     1141 cacggtcgat acgtcctgaa gcaactgtac cacgtccagt gattgagaat acgtcctcga
     1201 ctggaagaag caatggtttg tcagtgtcac gttctggttc tgggatatac tcatcaactg
     1261 tgttcatcaa ttccataacg atgtcttcgt atttagagtc accttcaaga gctttaagtg
     1321 ctgaaccttg gataactgga agatcgtcac ctgggaagtc gtattctgac aataggtcac
     1381 ggatttccat ttcaaccaat tcaagcaatt cttcgtcgtc aaccaagtca actttgttca
     1441 tgaagacgat aaggtgttta acaccaacct gacgtgaaag aaggatgtgc tcacgagttt
     1501 gtggcattgg tccgtcagtt gaagctacta caaggatagc tccgtccatt tgagcagcac
     1561 cagtgatcat gtttttaacg tagtccgcgt gtcctggagc gtcgatatga gcgtagtgac
     1621 gtttttcagt ttcgtactca acgtgcgcag tgttgatagt gataccgcgt tcgcgttctt
     1681 ctggagcagc atcgatagac gcatagtctt taggttggtt aactgatgaa ggcaagcgac
     1741 gtgccaaaac agttgtgata gctgcagtta gggtagtttt accgtggtca acgtgtccga
     1801 tagtaccaat gttaacgtgt ggtttactac gatcgtattt ttcttttgcc atttgagtaa
     1861 aagcctccaa taaaatatat tttatagata gacagtaggc aatacagtct aactttcctt
     1921 actattttat caaatttcag ctaaattgca agtgttttac aacgtttttg tgaattattc
     1981 ttggtaaaaa tttaactaat taatagaaag tactaaatag acttatcctt caaggagttt
     2041 tcagcattat ccatccactc acttgctgaa aatgtaaaca gtctctggtt tatatagtaa
     2101 aatgaaacaa gaatagggca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt
     2161 agaagtagat gtgtactatt ctagtttcaa tctactatac aacctcaaaa ctgtgttttg
     2221 aacaacctgc ggctagcttc ttagtttgcc ctttgatttt cattgagtat aaaattgcag
     2281 ataaaacgat aacaatcata ggactcagca gagtcaatgt aggtcgaaga atagcccccc
     2341 taatcgctta ttttatagca aaatgaaata gaaactgaac attttgttga ggaaggtaaa
     2401 tcaattcctc acaatgtttg agaaagaagg ggggactatt ccaacttcaa cccactataa
     2461 agcagactta caagatagag aacccatctc cgataatata tagtagaatg aaaactgcac
     2521 aatttgattg gagaattcaa accaatttct aacaatgttt tagaaacaag ggtctattat
     2581 ttcaactgca acctactata gcccattgaa gctggaatag cccactgaga cttctaaacc
     2641 actgctggag aggagtttgt ttagagtttc tttcatttcc ctaaaaaatc aaaaagagag
     2701 aaccagtctg atcctctctt acttatagat ggtgctcact caaatgttaa aggagctatt
     2761 tcttagagat aaaggaattt gaatacagct actgccgcaa ttgctgctgc gataggtgct
     2821 actactggta cccaagaata ccaccatttt gaatcgcctt tatgctcacc aagaactgat
     2881 tttggaagga aagcatgaag gagacgtggt cccaagtcac gggctgggtt caaggcaggt
     2941 cctgtaggtc ctccaagtga tgttaccaaa gccataacga ggaatccaag tgccaagtga
     3001 gccacagaaa gtcctgaagc agtgtgtgga gccacctgtg ctttaatagc caagtcagaa
     3061 aaatcaactg tttgtcctgc ttctattgcc ttttgtttca tgaattgaag cacttcagca
     3121 ccaaagaagt ttttagtcaa accaagagct gcaaagaaca aaacaaatga accaacaaac
     3181 tcattaatca aaccattgac agttgctgca tagcgacttt cttttgtacc atggtcaata
     3241 cttgaaatag ttgagaaagt tcccaagatg ttatttggtt tttcagtttt caagtagaat
     3301 ggacggtatg ttgccacaac taaggcttgg ccaaagatag cccccaagac ttgcgcgata
     3361 atgtaaggta ccacttgtgc ccaagggaaa agaccgctaa ctgcaagccc tagagtgaaa
     3421 gcagggttga tgtgattccc agatacgtta ccaaacatca aggctgggat cataaccccc
     3481 ataccataac caacagcgat gacgatccag ccactttggt gacctttcgt acctttaagt
     3541 tcaacgttgg caactgcacc attcccaaga atgatcaaaa tggcagttcc caaaaattca
     3601 gtggcatact tcagtgccca tgtgaaatcc atttgctaga ttctccttaa aattttttag
     3661 atattcctta ttttatcaat ttttacccta tttagcaact gatttctgga aatattttta
     3721 aaaaaatagg attcatagtt tcccagttca acgtacttgt aagcgttttt attcttcacg
     3781 aaagacctca ttctcaaaac caggaaaaag cccacatctg tgatcttttc cttgttccta
     3841 gattctaaaa tctattattt tttatcttct tttttcttga agaatcctaa tcctagacca
     3901 gctaacaagg tcatgatacc agcactagcc aaggtagcat tagcttctgt acctgtgttt
     3961 ggcaattcct ctctcttacc tgtctcataa gtcggaactt ctgggtctgg attcactgga
     4021 gtttcagttt ttggagtacc tggttctgga gttggtttat ctggtgttga taaacggtca
     4081 taccttaccg ttatttcttt atcactagag tctgacgtaa cttcttgtga ttcaactgtt
     4141 ggaatatctg gatctttgta cttgtcaatc ttaccagata taacctcgtc ccagtttcct
     4201 gttgtccatt caccgtaggt tacaactccc gtgaccttgt tctcagtttt tgtacggctt
     4261 aaggttacag gttgaacaac atcttctttt acattttggt tcgtaacttt atcaacgtaa
     4321 tgaatgatac gcgttatagt cttcgtctca gtagaggttg ctgttttggg aaccactgtt
     4381 tcctcaacat tctcacggta gtaatagtca actgttgcac cgtcttctgg tacgcatttg
     4441 caggagttgc taccaaggtg tatgttgttt tccttgtgat aactcggtct tctttgtcct
     4501 cagttgttgt tttcccttca atagtttttg attctgtggt atactcagaa cctatcgcta
     4561 aatcagcttt tataacagac tctaccaact tctcttgcct accttcttta tagtaattgg
     4621 ctgttactgt agcagtggtt ggcgcttcgc tttctctata aactaaggtc actgttctac
     4681 cttcgcttac aatattccca gttaaacttg cagaatttgt atctgcttct ttaaaagtat
     4741 aatattttcc gtcagtagta gtcatgctac tgagtttttt atctgtgaca ttatagctgg
     4801 taccaatcag ttgtttttta ttggtaatgt aggttccgtc actttctttt tctccaattc
     4861 cagtatcatt tttcaatgat agcaaacgcc cttgttcatc aacatagcga actttcacat
     4921 tttctgagat tagttctgcc aattctgagg tttttttctt tttcttgatt tcttcggtta
     4981 ttttcccttt ctcttcttcg ggaatattta gttttggaat gattttttca acaacggttc
     5041 gtgatggttc cacagtatct tggatgactg aaaagtcagc tagaattggg agattatact
     5101 gaacacggtg actttgagtg tttactccta ctctttcatt attctctgaa aatacttgta
     5161 cggtataaga aacaacatct tttcctaata gaacatcccc agtagagaaa tagccgcctt
     5221 ttcctagttt gctatctcca gagcccactt ctttcctaat cttatcagat agttttttac
     5281 cagtcagtac attcgttcgc acaatccctt tgtctacccc tacaaagtgg gagaactttt
     5341 tgaactcttc agaaccagat ctagcccaac cattattaag ggcatttgct tttgtatttg
     5401 tattctctct caaaagtttg gcgattagaa ttatattcat cggcacttag agttgctgct
     5461 atatctgact cttgaatacc aacttcctta ctaccatttc tagcggcagt atatgtgtat
     5521 ttatctgttt ctacttctag ctgattatcc ttaacatgat aggtaatttt ctcctggtat
     5581 aagagtcttc agtagtttat atactctgtc atcatcttta gctttagaag cagctgagtt
     5641 tagccaagtt ggggtattag gagtatcttt atcagcctgt ttcaagacat tattcaagtc
     5701 atttgctagc ctattggtta tcctcactgt gaaagttttc ttccagtctg aaccactttt
     5761 attagactta gatatgctca tgaaatcctg gtcaacattc aacacaatat cgccttgttt
     5821 tttagcatct tccgttgctt ctgcttcaaa ttgatcatgg agtttttgtt gctctggtgt
     5881 atagtttttt tatttacacg atttaatgta ctatttttta ttttagtatt aagaactcca
     5941 ttaatacgat ttttaaactc tgcttcaaaa aatcccttaa tttgttggtg cgcaagttca
     6001 acattcaaac ctttcttata caagtcttca ttctgataca agtcttcatt ctgaacgatt
     6061 tcggctacac ggtgaatggt ttgattgtat accgtcttct cttaaccagc agtatcctta
     6121 gttttaatga ctactcgata ggttccttta gaaccgttat agtcatctag ttttaaaaaa
     6181 gcattccaaa cattcaaaaa tagttggaac ttcccttaat acatccgtca ctttcttttt
     6241 caagatagcc caagcttgct caataggatt caaatccggt gaataaggtg gtagaggtaa
     6301 gaaaaagtgt ttatcttgga tgcagagttc atccaaaatg ttcttgggat gaaaaccagc
     6361 attgcccatg acaataacat gaggtgtctt caaagcaggt aggagttgcg ttttgaacca
     6421 ctccacaaag aaatcgcttg tcatgctttt cttgtaaatc atgggagcta taaactctcc
     6481 gtctacttgt cctgcaacaa ttgaagttcg ctcaaaacga cgtccgctaa tcttttcata
     6541 gactttctcc cctctaggag cccctgcata aggacgatag agatagcggt cgattcctgt
     6601 ttcatcaata taaacaggtg ctaggtgctt taaactatta aaattcttaa gaaataaggc
     6661 tacttttctg ggtcttgttc cttaaagctc gtcatctttt tttaaagtga ccttaatctg
     6721 ctttaaagct gcccatactg aaggaacagc acaatcaaaa tgtgccgcaa tttcccgtaa
     6781 aaaagcatct ggatgagcct ctacaaaggc tttcaattcc tctaaaggaa tctttcggtt
     6841 tttgacgact cgctttttcc tctctatgtg ttcttgttca cgtctttctt ttcccacgtg
     6901 aagagagttc tgacgccaac accaaaaaac ttggctgcct cgacatggct gtgcccctct
     6961 ttgatgtaat ctaatgctcg ctgtttaaaa tcagtactat atgccatagc tttattataa
     7021 tatgcttgtt taaaactaag taactatatc aatgttttgt tatgagaaga cttacttttt
     7081 ttactgaaat tgaatttttc ctagcctcaa catcatataa ccccaaaatt caatctgacc
     7141 tggtttgaag agatgttcga agcagataag gttttactaa aactaaaaaa aatgatataa
     7201 ttaaactgat taaatgtttt aaaaataaag gagaatttga atgtcttacc aagaaaatta
     7261 ccagaaatgg gttgattttg tggagcttcc tgactacctt cgtcaagatt tggaaaatat
     7321 ggacgaaaaa actaaggaag atgccttcta tacaaatctt gaatttggta ctgcaggtat
     7381 gcgtggcttg gttggtgctg gtacaaaccg tatcaacatc tacgttgttc gccaagctac
     7441 tgaaggattg gctcgtttga ttgagtcaaa aggtggaaac gagaaagaac gcggtgtagc
     7501 aattgcctac gatagccgtc acttctcacc tgagtttgcc tttgaatctg cggcagttct
     7561 tgctaaacac ggcatcaaat cttacgtatt tgaaagcctt cgtccgactc cagaactatc
     7621 atttgcagtt cgtcatctca actgtttcgc aggtatcatg gtcacagcca gccacaaccc
     7681 tgctccattt aacggttaca aggtttacgg tgaagacggt ggacaaatgc ctccacacga
     7741 tgcggacgct ttgactactt atatccgtgc aatcgaaaac ccatttgcag ttgaagttgc
     7801 tgatgtggaa actgaaaaag cttctggctt gattgaagtt atcggcgaag ctgttgacat
     7861 agaatacctt aaagaggtta aggacataaa catcaaccca gccttgattg aagaatttgg
     7921 taaagacatg aagattgtct acacaccact tcatggtact ggtgaaatgt tggctcgtcg
     7981 tgctcttgcc caagcaggat ttgactctgt tcaagttgtt gaagcgcaag caactgctga
     8041 cccagacttt tcaactgtaa catctccaaa cccagaaagc caagcagcct ttgcccttgc
     8101 tgaagaactt ggtcgtcaag ttggtgcaga tgttcttgtc gcaactgacc cagacgctga
     8161 ccgtgttggt gttgaagttc ttcaaaaaga tggtagctac ctcaaccttt caggtaacca
     8221 aatcggtgct atcatggcta aatacatctt ggaagctcac aaaaacgctg gaactcttcc
     8281 tgaaaatgcc gctctctgca aatctatcgt ttcaactgac ttggtaacga agattgctga
     8341 aagctacggc gcaactatgt tcaacgtctt gacaggtttc aaatttatcg ctgaaaaaat
     8401 ccaagaattc gaagaaaaac acaaccacac ttacatgatg ggatttgaag aaagcttcgg
     8461 ttacttgatt aaaccatttg tacgtgataa agatgccatc caagccgttc ttgtagttgc
     8521 tgaacttgct gcctactacc gttctcgtgg tttgacactt gctgacggta tcgaagaaat
     8581 ctataaagaa tatggctact acgcagaaaa aacaatctct gttactcttt caggtgtcga
     8641 tggtgctgaa caaatcaaag cgattatggc taaattccgc aacaatgctc caacagaatg
     8701 gaacgcaaca gctatcactg tcgtagaaga cttcaaggca caaactgcta ctgtcgctga
     8761 cggtactgtt acaaacttga caactcctcc aagtgatgtg ttgaaataca cacttgctga
     8821 cggttcatgg attgccgttc gcccttcagg tacagaacca aaaatcaagt tctacattgc
     8881 agttgtaggg gaaaccaacg aagaatcaca agctaagatt gctaacatcg aagcagaaat
     8941 caatgcattt gtaaaataaa gacctataaa agatgagacc aaccaacctc atcttttttc
     9001 gtatcaaatc taagcaattt tagaaacttt atggcatact agacttatca atgaaagcga
     9061 ggtaatctca tggaacaatt tttagataac atcaaagacc ttgaagtcac tacagttgtg
     9121 cgtgcgcaag aagctcttga taaaaaagaa actgcaacct tctttatcgg tcgcaaaact
     9181 tgcccttact gccgtaaatt tgcaggtaca ttgtcaggtg tcgtagctga aaccaaagct
     9241 cacatttact tcatcaatag tgaagaacca agccaactca atgatttgca agcattccgc
     9301 tcacgctatg gaatcccaac tgtaccaggt tttgttcata ttacagatgg acaaatcaac
     9361 gtccgttgcg actcttcaat gtcagcacaa gaaatcaaag atttcgcagg attgtaaaaa
     9421 atcagctaac aagtcttaaa atcacaaaaa cgcataatgt aaggtgcaaa aaccttgata
     9481 ttatgcgttt tattgttcct aaaacagtta catcagtatt tcattacaga acattttaat
     9541 tttggaagtc aaatcaattt ttaacaatgt tttagaaatc acagtgggct attccatatt
     9601 caattatcca tattcaatcg ctattatcgt tttcctagat tacaaaaaaa actacgtttt
     9661 gctaatcaaa acgcagtttc agccactatt ttatcttact gtccttcttt tacttctttg
     9721 gttgctacat cttctccaaa ccatttttgg ctgatttctt ggaacttgcc gtccttgtaa
     9781 agactagaaa aagcttcatt tatcttctta accaagtttg tatcttcctt acgggctcca
     9841 accgcaaaag cttctgtttc tagtccaact gtaaagacat tataatcgtt taaaacacct
     9901 tctgcttcta aataatagtt tgcatagaca cggtcaatca atagaccatc aattcgatcg
     9961 tttttcaaat caatcaaggc ttcattaaag gtttggtatt gattcgcttc cttattagcg
    10021 acaatattct tcaaaatttc tggatttgct tcaaagtccg cataaccaga tgaaccagct
    10081 tgagctccta atgtctttcc agtcatatcc tttgcagtcg tgataccaga tgatttcttc
    10141 gtaaccaata cctgctcatt cttcatatat gagttactga aagccacctt ttcacggcgt
    10201 tcgtctgtag cggaatagcc attccaaatc agatcaatcg ttccttttgt caattcagct
    10261 tctttcaaat cccaatcaat cggttgccaa tttaccgtga ttccgtattt ttcaaaaaca
    10321 gctgtagcta aatcaatatc aaatcctgca taagaaccat ctttctgagc aaatcccatt
    10381 ggaacaaaag tactatcaaa tccaatagta atagacttgt tagactggta ctttgaccaa
    10441 ttatctccac tagtttcgct agaatttttc ccacaagcta ctaagaacaa agcagtcatc
    10501 agactgacta atacaagcat ccattttttc atccattttt cctcctattt aggttctact
    10561 ttcaataata catctgcgat attttcagca aactgcaaat catgggtaac cacaatctgg
    10621 gtcatcccaa gttccctatt ttgcaagatt agcttctcca cttccaaacg taattctgga
    10681 tccagggcag aagttggttc atcgtagcca atgatttctg ggtcaatcat catagcacgc
    10741 gccaaagcca cccgctgctt ttgcccacca gatagtgaga aaggataggc ctctgcgtgt
    10801 cctcctagtc ctaactgttc caagagtcca ctcgccttct tctcagcctc ttcctgcttc
    10861 attcccatgg tcttcacagg cgataaagtc aaattttcca gaactgatag atgaggaaat
    10921 agttgaaaat cttggaagac aaatcccagt agattgcgct tctgcaattc atccagctct
    10981 aaaggttgtc cattataaaa gatttgccct gaatcaatgg tttcaagacc tgcaagcata
    11041 cgtaaaagag ttgtcttacc tccaccagaa ggtccaacga tagccaggat ttgcttttca
    11101 ggaatactta gactgaaatt agacaggatt tgtttgtctc caaagacttt attgatattt
    11161 cgtaattcta acatggcagc ctcctatcta taataactat acttcttctc aacttttttg
    11221 gaaataattg tcacaatccc aatcaaaatc aaataaatgg ctcctgccaa gaacatagga
    11281 actagactag catcgcggtt agcagctgtt cgactagcca agataaggtc tgaaattccg
    11341 agagcataga ccaaagaagt atccttgacc aaactcataa cttcattaaa gacactagga
    11401 agaacgatct tggtcacttg gggcaagata atatagcgca ctctgtcaaa agggctaaac
    11461 ttcaagacct tggcggcctc atactgtcct cttggaatag tgtcaattcc cccacggaaa
    11521 atttctgcaa agtaagctgc atagttgaga acaaaggcaa taatagctgc aggaaggcgg
    11581 tctaaacgaa tcccaatact tgggagcaca taatagataa aaatcagttg caagagtaag
    11641 ggggttcctc gcataaccca gatgtaaatg ttaatcagat aatggagggg cttccaatgg
    11701 acttgcaagg caaaggcaat caaaacgccc aagggaatcg aaaatagcaa gaccagtgca
    11761 aagacctgta cagtcgtgct cgcaccattc agtaaactcg gtaatatctc aaataaataa
    11821 gacatactgc acctcctaaa agtaattgtt cctattatag catgaataaa caagattacc
    11881 aagtattttt gtaaaaagat tctaccatat acaaataaaa accgtagcta tcaaaagaga
    11941 taaactacag ttctatatgt ttaaacattc ttacaatctc tcaaacagtt cttgcatttg
    12001 cacatcatct ggaaccagtt ttaagtaagt gtgagcatgg acttttgctt cttcaaaatg
    12061 tcccaattca cgcaagagat agatatagtg ttccagaaat tctggattgt ccttcaaatc
    12121 tcctgtcaac tcttgataat actcataagc agtatccaaa tcgtccattt cttgataaga
    12181 acgagcaatc atccacttgg tcaaaagatt ttctggctcc tcactctgca attctagaat
    12241 atcctcataa cgctcctgct ccagataaat agtggctaaa cgaagcaaga tttcttctgt
    12301 atcctcagcg tcttcttttg cagtaaggag ataattttct gcaccactag catcatgcaa
    12361 ttcataagaa aattgtgaag cagctagcaa gaggcgagtt tcaaagggat ttttctctaa
    12421 tccttgctta gcgatacgca gggcttcttg aacttgatgt tccttatgta aagcctgact
    12481 gtacccatac tcatagcctt caaagtcagg agaaatggta tcaagctgct taaagtagag
    12541 ggtggctttt tgatattctt cttgatcaaa ataaagactg gccaactcaa aagctgttaa
    12601 gtcatcgtat tctaactcca gggctttttc taaaaactca gtagccgttt caaatttccc
    12661 taactgagca taggcaaagc caattcgttg ataggtggaa atgcccgttt gctcataaat
    12721 cgagcgatta tctaactggg cataggcttg aatagccgct tggtaatttt ccaactcact
    12781 atccaactct gccaaaccca atatcaagag agaatcctct gagtaggtca aggcctccaa
    12841 taatttctca cgtgccacat ctgtcaaacc ttccagctgg taaaggtctg ccttcagagc
    12901 caaagacgag acataccagt cactgtcagc ttggatttcc tcaagatagg taaaggcttc
    12961 ttctatttga ccatcctcgc tagcaattgc agctagatta agatgaacct ctggaaaatc
    13021 ctctacaatt ttcaggtaaa tttccttggc ctgaggatag aaaccaatcc cttcaagata
    13081 agttgccaat tcatacagaa gatcacttga atcattttct aaagctttgg cgaaataatg
    13141 ctcagcctta gttaaatctt gctcctccaa agcctgtaac atctgttgac tattgttcac
    13201 tcttgacttc ctcaccctct aattcatata gaccactgac taccttatac cattcgaaaa
    13261 tggctgaaat gacaacctta gcagaggcat aaaccggaat accaagtaaa actccccaga
    13321 taccaaacat agatcctgaa gttaacaaaa caaagagaac attaataggg tggatgttta
    13381 attgacttcc caaaatcaat ggagagacaa aacggccttc aatagtttgt tctacgataa
    13441 agacaatcac tactttcaaa agcatgactg gaccagcaat caaacccaat actagagcag
    13501 gaagcatggc tagaaagcta ccaagataag ggaccagatt taaaatacca gcagtaaccc
    13561 ccagcgtaac cgcatagcgt agaccaataa tcttgaagaa gatgataaac attactgcta
    13621 caataatagc cactgtcact tgccctcgaa catagttgga caactgttga ttcacatctg
    13681 ataaaacttg tccaacaggt tccttcaatt ttcttggaat gaattgggtc aaatagttac
    13741 gcaagccttt cccatcacgc aagagataaa agagcatgaa aggaacgata atcaaggcaa
    13801 caatcacttg agaagccccg ctaataaagg cactcaccca gttgactgcc tgagatgaaa
    13861 ccttacttgc caaaactgta gcctggctag aaaaattggt caaaacttgc tctaattgag
    13921 gtctgaaatc atctggcagg tgctgggcta ccaatccatt aacaatccta tctatatctt
    13981 ctaagtaaac aggaacgttt cttgcaaagg tcaaaacctg acgttgcaga tttggaatgg
    14041 cgactgccaa gccccaaatg ataaagagag cgatgataac aaagacaata gtgatagcta
    14101 taacacgatt aaccttatgc ttctccatcc aatcaacaat aggattcaac aaataatata
    14161 acaaaccaga caaaatgact ggcaacatca caactgctaa aaagtctaaa acaggtgaaa
    14221 atagaaaact aatcttactt aaaataaaaa gattcagtcc caataataag gttactaaaa
    14281 ataccgtaat tgccttgtta tctaaaaacc acttgaaaaa ccaagatagg ctaaaatgtt
    14341 tctctttttg ctccataaat acttcctttc tattgttctc tcattatacc atttttgaaa
    14401 caaaataact cttttttaaa gtgcttacat ctaattggat atctttaatt tacttgtttt
    14461 tgtgatataa tagtcttatc tttacacaga ggtatagtaa tgaaagaaac tgtttatttt
    14521 ggaacttata cacgtcgtac ttctcaaggg atttacaagg cagactttga tacagaaact
    14581 ggtcagcttt caaatctaga actttttgca gctgagccaa gtccaaccta ccttgccttt
    14641 gaccagcacc aacatttata cactgttggt agccaagacg ataagggggg aattgcagcc
    14701 tatcaaactg acgggactgt gttaaatcat gttgttgaag aaggagctcc ccactgttat
    14761 gttgctgtcg atgaaaagcg tgatttggtt tacgcagcta actatcacaa gggacaagtc
    14821 cttgtttata aacgccagga agatggtagt cttctactta gtgatatgga tcaacacagt
    14881 ggccaaggtc cacatgaaaa tcaagcttcc ccccatgttc actatacaga tttaacacct
    14941 gaccactatc tagtgacctg cgacttggga actgaccaag tcatcaccta tgacctcgat
    15001 caagaaggaa aattatctaa gctctatacc tatcacagca agccaggagc aggctcacgc
    15061 catatcattt tccataacca ctataaaatc gcttatctca tttgtgaact caatagtact
    15121 atcgaagttt taatctacga tggcgttggc gaatttgaac gtatgcaggt catttcaact
    15181 ttaccggaag cttacgaagg ctttaatgga accgctgcta ttcatctctc taaagacggt
    15241 aaatacctct acgcttctaa ccgtggccat gattctatcg cagtatatac catccttgcg
    15301 gacggtagct tagagttatt agaaatcgtt ccaacgcatg gtcagactcc acgtgatttt
    15361 gatttgacac ccgaccaaaa atttctcatt gttgtccatc aagactctga caatgcaact
    15421 gtctttaaac gtaattgtga caatggtcgt ctagcagaac tctccaacga cttccatgtt
    15481 cccgaagcag tctgcatccg ttttgctcct taaaacacca taaaaaatga acttggtatt
    15541 ttcaagttca ttttttctta tagtctattt ccgacattaa tacggttaat agcacgttgc
    15601 aaagcaatct tagcacgacg ttcttggtca atcaaatgtt tgtcttgtgc ttcttcaatt
    15661 gcacgttctg cacgaagttt ggcacgttct gcacgactga tatcgatatc acgagcacgt
    15721 tctgcagagt cagcgacgat tgtgatcata tcattggcaa tttcaataac gccaccgttt
    15781 actgcaatcc agttcacgtg atctttatca tcgatacgtt ttacctttac ttcatcaact
    15841 gctaaaaccg caatcatatt ttcatgtcgt ggcaagatcc ccatctcacc atccagagtt
    15901 cgaaccgata catagctggc atggtgatca tagacgagac catctggtgt cacgatctgg
    15961 acagttaact gagccataga tcacctctta aaatcccatt ttttcagctt ttgcaatcac
    16021 atcttcgata gaacctacac cacggaaggc atcttctggc aagtggtcgt atttaccatc
    16081 aaggatttcc ttaaagccac gtacagtttc agcaactgga acataagaac ctggctgacc
    16141 agtaaattgt tccgcaacgt tgaagttttg tgacaagaag aactggatac gacgggcgcg
    16201 agcaaccaag gtcttttctt catcagaaag ctcatccata ccaaggatag caatgatatc
    16261 ttgcaattca tggtaacgtt gaaggacacg ttttacttca gcagcaactg catagtgctc
    16321 ttctccaacg atttcaggtg ccaaggcacg tgagcttgaa gcaagtgggt caacggctgg
    16381 gtagataccc aattgtacca acttacgttc caagtttgtt gttgaatcca agtgagcgaa
    16441 ggctgttgct ggcgctgggt cagtatagtc atccgctggc acatagatag cctggataga
    16501 ggttacagaa cccttcttgg ttgatgtgat acgttcttgc aattgaccca tttccgtagc
    16561 aagtgttggt tggtaaccaa cggctgatgg catacgaccc aaaagggcag atacttctga
    16621 accagcctga gtgaaacgga agatattatc gataaagaga agcacgtctt ggccttccac
    16681 atcacggaag tattcagcga ttgtcaaacc agtaagggca acacgcatac gtgctcctgg
    16741 tggctcattc atctgaccaa agaccatggc tgttttctcg ataacgcctg attctttcat
    16801 ttcccagtaa aggtcattcc cctcacgagt acgttcccca acaccagcaa atactgaaat
    16861 accaccgtgc tcttgggcaa tgttgtgaat caattcttgg attaagacag ttttaccaac
    16921 tccggcacca ccgaaaagtc caactttacc acctttaagg taaggggcaa gaaggtcaat
    16981 aaccttgatc cctgtttcaa ggatttcaga agaggtagac aactcatcaa aagttggagc
    17041 ttttttatga attggctgac gctctgcgtc ttctgtaaaa ggagcttcca agtcaatggt
    17101 atctcccaaa acgttgaaga cacgtcccaa agtttcttta cctactggta cagagattgg
    17161 acgacctgtg tccaatactt ccattccacg agtcaaccca tctgttgatt ccatggcgat
    17221 agtacgaacc ataccatctc ctaactccaa ggctacttca aggacgattt ttgtttttct
    17281 ttcgtcattt ttgtagacga caagtgcatt gttaatctca ggaagttttt cccctgctgc
    17341 aaacaaaacg tctacaacgg gaccgataac ctgagcaatt ttacctgaac tcatctcctt
    17401 ctcctattct atataagata ctgtcggttc ctagctcaac taggtcctaa gttcattttc
    17461 atacgagctg gactagagcc tattctaagg cactagcacc tgctacgatt tctgtaattt
    17521 cttgtgtaat cgccgcctgt ctggcacggt tatactgaat tgtcaaatca ttgatgactt
    17581 tcttagcatt atctgtcgct gtttgcatgg ctgtcatgcc cgcagcattc tcagctgtct
    17641 tggcatcgat aatggcaccg taaatcatac tttctgcaaa ctgaggcaac aactgctcca
    17701 gaatttcttc tcggctggtt tccaattcaa aagtcaagct gtactcttca tccgcttcat
    17761 ttggatccaa gtcaacaatc ggaagcattt gttccacacg catttgactg gttagcgtat
    17821 tgacatggtg gttgtagcaa acataaagct catcaaagag ttcattttgg tacatttcaa
    17881 cagtttttga aataatctta cgaacttgat caaagctagg ttggtctgac aagccacgta
    17941 attcataaag tggttgaata ccgcgagcct taaagaaatc agctcccatc ccaccgatac
    18001 agatcatttc aaaaccttta ccgtctgggt ggtattcttc tttcaactcc ataacagctt
    18061 tcaaaataga ggaattataa cctccaacca aaccgcggtc tgaagtgata acgatatagc
    18121 ctgtcttctt cacagaacgg ctaatcaaca tcggattagt tgaagcacca gctccattac
    18181 catgaaggat atctgtcaaa agtttacgca ctttctgagc gtaaacttgg aagttgcgag
    18241 cagcttcttc agaacgacct agcttagcag ccgataccat ttgcatggca ttagtaattt
    18301 gactcgtatt ttttgttgag gcgatttttg ttttaatatc atttagagat actgccatct
    18361 gacacctcta ttcttattgg aagctagatt gattgagaaa ctctgtaatc gcagcatcca
    18421 agactgcttc ttctggcaag tcttttgtat cacgaatggt ttccaaaatc tctggatgtt
    18481 gagcatcaaa gaaggcatgg aactcttcct cgaaacgaac aatatcatct actggaacag
    18541 tatccaagaa accatgtgtc aaagcataaa gaatggttac ttgtttctca acaggtaatg
    18601 gtttgtgaac aggttgtttc aagacctcaa cggtacgacg tccacggttc aacttagcct
    18661 gtgttgctgc gtccaagtca gaaccaaact tagtaaaggc ttccaactca cggtatgaag
    18721 caaggtcgat acgaagtgta ccagcaacct tcttcatggc tttgatttgt gcagaaccac
    18781 ctacacgaga tacagatgaa cccgcatcga tggctggacg aatacctgca ttgaagaggc
    18841 catcgccaag gaagatttgt ccatcagtga tagaaatcac gttggttgcg atataggctg
    18901 agatatctcc tgcttgtgtc tcgataaatg gtagggctgt aattgatcca ccaccaagtt
    18961 catcagaaac tttagctgag cgctcaagca aacggctgtg gagatagaaa acatcccctg
    19021 ggaaggcttc acgacctgga ggacgacgaa gcaagagcga cagttcacga taagctaccg
    19081 cttgttttga tagatcatcg tatacaatca aaacatgctt accttgatac ataaattctt
    19141 ccgccatagc aaccccagca taaggagcta ggaagagcaa tggagatggt tgtgaagcag
    19201 aggctgtcac aacgattgtg tagtccaagg caccgtactg acgaagtgtt tctacttgcg
    19261 tacgaactgt tgattctttt tgtccaatcg cgacgtagat acagatcata tcttgatctt
    19321 tttggttcaa gattgtatca atcgcaatgg ttgttttccc tgtctgacgg tcaccgataa
    19381 tcaactcacg ttgaccacga ccaatcggta caagggcgtc aatagctttc aaaccagttt
    19441 gcaatggttc tgaaacagac ttacgttgca taacaccagg agctggtgct tctactggac
    19501 gagttttatc agtgtggatt tctccaagac cgtcaactgg acgaccaagc ggatccacaa
    19561 cacgaccaat cagactttca cctacaggga cttccatgat tttccctgta cggcggattg
    19621 tatcgccttc acggatatct gtaaagtcac ctaggatgat aataccaacg tctgttgact
    19681 ccaagttttg agccatacca taagagccgt tttcaaaatt caacaactct ccactcatga
    19741 cattttcaag gccgtgagca cgcgcgatac cgtccccgat ataggttaca acacctgttt
    19801 cagtcacatc aaaattgggt ttgaaatttt caatttgttg cttaattaaa gcgctgattt
    19861 cttgtgcgtt aattgccaaa agaacaccac tttctatttc aaattttctt taacaacttt
    19921 aagttgttgt ttaatactca catcaattgt cttgtgattg gcaaaaatga caaaaccacc
    19981 aatgagactt tcatcgattt gttcttttac actccttact ttcagagaca tttttttctc
    20041 aatcaaaggg agcaaacgag tcttctgttc atcagttaga ggatgagcag acgtaatcgt
    20101 cacttcaaat cgatttgttt ctttttcaag tcggttcaag caatctacaa gcacatcata
    20161 aaaaagattt gctctgtgat tgtaggccag aacctggata aagttttgta ataaaggtga
    20221 cacagaatct tggaaaaaag caattgtttt ttccttatcc gactcgtcta ctgccacttg
    20281 ttttaaaaaa gaaggcagac ctgttttttc aacaacttgc ttgatttgag tcaagtctga
    20341 aaagatacgg tcttcttctc ctttttcaag taccaattgg acaaaaggca tgctgtattt
    20401 ttcaattacc tttactgttt tcttgtccat taagcttctc ctagctgatc gatatactga
    20461 tcaatgagtg ctttatgggc atgactgtca aggttttgtg agatgatttt accagctaag
    20521 ctgatggtca aatctgcgac ctcaccctta acactctgta aagcttctac tttattttga
    20581 gcaatttctt gattggcttt ttcttttaag tgtcctgctt ctagtttagc atctgctaag
    20641 atattagcct tactttgctc agctgtttcc tttgcatttt caatgattgt cttagcttct
    20701 ttacggctac cagccaattc atcttcgcgt ttttgagcca atacttctgc tttttgacgg
    20761 gcttcttcag ctctgtcaat atctgaagca attttttcag ctctttcttc gaaaatgcct
    20821 gtaatattag accatgcaaa ttttttaatc aagactagca aaagaataaa agagccagtg
    20881 attaaaataa aattaccaat taattcacct actgttacgt gcatcagtta ctcctttcta
    20941 ttcctcttca atattaatct tattccctaa atacacagat gtcaaaagag taaaaacata
    21001 agcttggatg caggaaataa agacagaaaa tgcagtccaa gccaaattag ctccaaaggc
    21061 tactggatac caataaatag cttggtggga aagaagaagt aacaaactcg tcatgacctc
    21121 tcctgcaaag atattcccaa aaatccgcaa agccaaagat aagaagtttg taaattcttc
    21181 caagatattc atcggtatga caaaaacagg actcataaaa gattttatac tttttttaaa
    21241 tccacgacga cgaacgcttt ctatatgtgt caacaaaatg acaagaaaag ataaggttaa
    21301 gtcatactgt aaattagcgg ttggcgaact ccaccagtta gtcccatcga tcgtttgaag
    21361 ctttgtcatt aagccaaggt tattggcaat caccatgaaa aggaataaac aaaggaaaaa
    21421 gagtgagtaa tctttcatgt agcgcgaacc aatgttaggt tctgtaaatc caataacaaa
    21481 gtcatagaca tactcaagta cattttgctt tcctttgggt ttcaaggtca tattgcggct
    21541 tgcccaataa ataaagacaa aaataactcc cacaatcaac aaagtcatgg ctaacatagt
    21601 cagattgaag ataacaggac caatagagat gattggatta atactttctt ccatctaaaa
    21661 tcctcccttt tcttctcgtt ccatgtattt atttgataat aaatgagaag acaagagtta
    21721 caaagaaagt tccttcaata aaggcaacac ctaaaaacat caaactacga aactcagaaa
    21781 gcatatctgg ttggcgtgct actgatttaa acagtccatt catcaataaa ccttcaccga
    21841 cagatacgcc catacaggca atacataagc ctaaaaatgt taaattcata acaaattctc
    21901 ctttaaaata aaatttactt acttagtgta gtcctaaaat ttaaatttgt caacttttaa
    21961 ccgctattgg aagcgtttaa taaaaattat ttccattttg ctaaatttca agttcaagtt
    22021 agccatccag aagtcttctc tgggtgactt gtagtccaag tattttttag gatagttgtt
//
LOCUS       DAVKJU010000033        21319 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVKJU010000033 DAVKJU010000000
VERSION     DAVKJU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.033"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            98..2320
                     /gene="recJ"
                     /locus_tag="VW191_001183"
     CDS             98..2320
                     /gene="recJ"
                     /locus_tag="VW191_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU9623956.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2495..4156
                     /locus_tag="VW191_001184"
     CDS             2495..4156
                     /locus_tag="VW191_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9623957.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4225..5004
                     /locus_tag="VW191_001185"
     CDS             4225..5004
                     /locus_tag="VW191_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEU9623958.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5116..6336
                     /gene="murM"
                     /locus_tag="VW191_001186"
     CDS             5116..6336
                     /gene="murM"
                     /locus_tag="VW191_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEU9623959.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6341..7573
                     /gene="murN"
                     /locus_tag="VW191_001187"
     CDS             6341..7573
                     /gene="murN"
                     /locus_tag="VW191_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEU9623960.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEYFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFCYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7652..8353
                     /locus_tag="VW191_001188"
     CDS             7652..8353
                     /locus_tag="VW191_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU9623961.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8393..10237
                     /gene="uvrC"
                     /locus_tag="VW191_001189"
     CDS             8393..10237
                     /gene="uvrC"
                     /locus_tag="VW191_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU9623962.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEIYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10227..10379
                     /locus_tag="VW191_001190"
     CDS             10227..10379
                     /locus_tag="VW191_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEU9623963.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLLLN
                     ENQRAN"
     gene            complement(10462..11262)
                     /locus_tag="VW191_001191"
     CDS             complement(10462..11262)
                     /locus_tag="VW191_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9623964.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQPQLQ"
     gene            11433..12038
                     /locus_tag="VW191_001192"
     CDS             11433..12038
                     /locus_tag="VW191_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU9623965.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12051..13451
                     /gene="pepV"
                     /locus_tag="VW191_001193"
     CDS             12051..13451
                     /gene="pepV"
                     /locus_tag="VW191_001193"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEU9623966.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFDFAGPAKEYLDIAGKILLNDHEGESLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            13505..14083
                     /locus_tag="VW191_001194"
     CDS             13505..14083
                     /locus_tag="VW191_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEU9623967.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKIARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMVKNLWFEAEVVPDLKKRIKTIL"
     gene            14243..14395
                     /locus_tag="VW191_001195"
     CDS             14243..14395
                     /locus_tag="VW191_001195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9623968.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            14438..14530
                     /locus_tag="VW191_001196"
     CDS             14438..14530
                     /locus_tag="VW191_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9623969.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            14701..16026
                     /gene="brnQ"
                     /locus_tag="VW191_001197"
     CDS             14701..16026
                     /gene="brnQ"
                     /locus_tag="VW191_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEU9623970.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16230..17267
                     /locus_tag="VW191_001198"
     CDS             16230..17267
                     /locus_tag="VW191_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU9623971.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRTVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            17269..17730
                     /locus_tag="VW191_001199"
     CDS             17269..17730
                     /locus_tag="VW191_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU9623972.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKTFTAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMKGHGLKGRGPVWWVPFEEMTSETCQAKPDEIKRLVKRLSSEVDKLLE"
     gene            17741..18457
                     /locus_tag="VW191_001200"
     CDS             17741..18457
                     /locus_tag="VW191_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU9623973.1"
                     /translation="MKKRYLILTALLALSLAACSQEKSKNEDGEAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KVMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AESGLSLEEYYGFEGGDYVD"
     gene            complement(18497..18634)
                     /locus_tag="VW191_001201"
     CDS             complement(18497..18634)
                     /locus_tag="VW191_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623974.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            18766..19191
                     /gene="rplK"
                     /locus_tag="VW191_001202"
     CDS             18766..19191
                     /gene="rplK"
                     /locus_tag="VW191_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU9623975.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFITKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19398..20087
                     /gene="rplA"
                     /locus_tag="VW191_001203"
     CDS             19398..20087
                     /gene="rplA"
                     /locus_tag="VW191_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU9623976.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            20306..20443
                     /locus_tag="VW191_001204"
                     /pseudo
     CDS             20306..20443
                     /locus_tag="VW191_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(20574..20720)
                     /locus_tag="VW191_001205"
     CDS             complement(20574..20720)
                     /locus_tag="VW191_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623977.1"
                     /translation="MKTKEQTRKLVSGCSKHCFEAVDRTDEVSNHTHGKATLTWFEEI
                     FEEY"
     gene            20734..21096
                     /locus_tag="VW191_001206"
     CDS             20734..21096
                     /locus_tag="VW191_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEU9623978.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFETNIDENITDILYVNGTAILYPYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6386 a         3916 c         4898 g         6119 t
ORIGIN      
        1 tactataaaa tgttataaaa aagcaatctg gatagagaaa acgtctaaat catgttataa
       61 tgaagcaata gaattcttag aaagagtgga tgtctttttg ataacaccta cttatgaatg
      121 gcagtttgcc ctgcaggtag aagatgcgga ttttacaaag atagccaaga aggctggact
      181 gggtcctgag gtggctcggt tattgtttga gagagggatt caggaccaag aaagtctgaa
      241 gaagttttta gaaccttcct tggaggactt acatgatgct tatctgctcc atgatatgga
      301 caaggcagtg gagcggattc gtcaggctat tgaagaaggg gaaaatattc tcgtttatgg
      361 agactatgat gcggatggca tgacttcggc ttctattgtg aaggaaagtt tggaacaact
      421 tggtgctgag tgccgagttt acctgccaaa tcgttttacc gatggctatg gccctaatgc
      481 tagtgtttat aaatacttta tcgagcaaga aggaatttcc ttgattgtga cggtggacaa
      541 tggggttgct ggtcatgagg ctattgcatt ggctcagtct atgggagtag atgtcattgt
      601 gacagaccat cattccatgc ctgaaaccct gccagatgct tatgctattg tccatcctga
      661 acatccagat gcggattatc cttttaaata tttggctggt tgtggagttg ctttcaagtt
      721 ggcttgtgcc ctgttagaag aagtgcaagt ggaattgctt gatttggtcg ctattggaac
      781 tattgcagat atggtgagtc tgacggatga aaatcgtatc ttagttcaat atggtctgga
      841 aatgttgggt catacccagc gcattggtct gcaagaaatg ctggacatgg ctgggattgc
      901 tgctaatgaa gtaacagaag aaacggttgg tttccagatt gctcctcgtt tgaatgcctt
      961 gggccgcttg gatgatccca atcctgccat tgatttgttg actggatttg atgatgagga
     1021 agcgcatgag attgccctta tgattcacca gaaaaacgaa gagcgcaagg aaatcgttca
     1081 gtctatctat gaagaagcca agaccatggt ggatcctgag aagaaggttc aggtcttggc
     1141 caaggaaggc tggaatcctg gggttctagg aatcgtggct ggtcgtttat tggaagaatt
     1201 gggacagaca gtcattgttc ttaatataga agacggtcgt gccaagggca gtgctcgtag
     1261 tgtggaagcg gtcgatattt ttgaagctct ggatccctat cgagacctct tcatcgcctt
     1321 tggaggtcat gcaggtgcag cgggtatgac gctggaagtt gagcaactct cagatttatc
     1381 tcaggttttg gaagattatg ttcgtgaaaa aggtgcagat gctggtggca agaataagtt
     1441 aaacctagat gaagagttgg atttggaggc acttagcttg gaaacggtca aaagttttga
     1501 acgtttagct ccttttggaa tggataatca gaaacctatt ttttatatca agaattttca
     1561 ggtcgaaagt gctcgtacta tgggggcagg taatgcccat ctaaagctga aaatttccaa
     1621 gggtgaggcg agttttgaag tggtagcctt tggtcaaggc agatgggcga cagagttttc
     1681 tcaaaccaag aatctagagt tagcggttaa attgtctgtc aaccaatgga atggccaaac
     1741 tgccctccag ttgatgatgg tggatgcgcg agtggaaggt gttcaacttt ttaacattcg
     1801 tggaaaaaat gcagtcttgc cagaaggtgt tccagtcttg gattttcctg gagaactgcc
     1861 aaatcttgcg gctagtgaag ctgttgtcgt aaaaaacatt ccggaggata ttactcagct
     1921 gaagaccatt tttcaggaac agcatttctc tgctgtctat ttcaaaaatg atattgacaa
     1981 ggcctattat ctgacaggtt atgggactag agatcagttt gccaaattgt acaagactat
     2041 ttaccagttc ccagagtttg atattcgcta caagctgaaa gatttggctg catatcttaa
     2101 tattcaacaa atcttgctgg tcaagatgat tcaagtattt gaagaactag gctttgtgac
     2161 gataaaagat ggtgtgatga cagtcaataa agaggcgcca aagcgggaga taggagaaag
     2221 tcaaatttac caaaatctca aacaaaccgt taaagaccaa gaaatgatgg cgctgggtac
     2281 ggtgcaagaa atttatgatt ttttgatgga aaaagagtag aagttaggaa agagttggga
     2341 aatcaactct tttttgaaaa cagaccttca ttttgaaaat catcaaaaaa atggtataat
     2401 ggtaggaaaa gattcggctg aaagtattag aacttttaga ataagagggt agagttgccc
     2461 tataatcaag ataaactaag attttggagg aaaaatgagt aatatcagtt taacaacact
     2521 tggtggtgtg cgtgagaatg gaaaaaatat gtacattgct gaaattggag agtccatttt
     2581 tgttttgaat gtagggttaa aatatcctga aaatgaacaa ttaggggtcg atgtggtgat
     2641 tccaaacatg gattaccttt ttgaaaatag cgaccgtatt gctggggttt tcttgaccca
     2701 cgggcatgcg gatgccattg gtgctctacc ttatctcttg gcagaggcta aagttcctgt
     2761 atttgggtct gagttgacca ttgagttggc aaagctcttt gtcaaaggaa atgatgccgt
     2821 taagaaattt aatgatttcc atgtcattga tgagaatacg gagattgatt ttggtgggac
     2881 agtggtttcc ttcttcccta cgacttactc cgttccagag agtctgggaa ttgtcttgaa
     2941 gacatcggag ggaagcatcg tttatacagg tgacttcaaa tttgaccaaa cggctagtga
     3001 atcttatgca actgattttg ctcgtttggc agagattggt cgtgacggcg tcctggctct
     3061 cctcagtgat tcggccaatg cagacagcaa tattcaggtg gctagtgaaa gtgaagttag
     3121 ggatgaaatt acccaaacta ttgctgactg ggaaggtcgt atcatcgttg cagctgtttc
     3181 cagtaatctt tctcgtattc agcaaatttt tgacgctgcg gataaaacag gtcgacgtat
     3241 cgtcttgaca ggatttgata ttgaaaatat cgtccgcaca gcgattcgtc ttaagaagtt
     3301 gtctttagcc aacgaaattc tcttgattaa gcctaaagat atgtctcgct ttgaagacca
     3361 tgagttgatt attcttgaga caggtcgtat gggtgaacct atcaatggac ttcgtaagat
     3421 gtcgattggt cgccatcgtt atgtagaaat caaggatggg gacctggtct atattgctac
     3481 ggctccgtct attgctaaag aagcctttgt tgcgcgtgtg gaaaatatga tttatcaggc
     3541 aggtggggtt gtcaaattga ttacccaaag tttacatgta tcagggcacg gaaatgtgcg
     3601 tgatttgcag ctgatgatca atcttttgca acctaagtac ctcttccctg tccaagggga
     3661 gtatcgtgag ttggatgctc acgctaaggc tgccatggca gttgggatgt tgccagaacg
     3721 catcttcatt cctaaaaagg ggacgaccat ggcttacgag aatggagact ttgttccagc
     3781 tggatcggtt tcagcaggag atatcttgat tgatgggaat gccattggtg atgttggaaa
     3841 tgttgttctt cgtgaccgta aggtcttgtc agaggatgga attttcatcg tggctattac
     3901 agtcaaccgt cgtgagaaga aaattgtggc tagggctcgt gttcacacgc gtggatttgt
     3961 ttatctcaag aagagtcgcg atattctccg tgaaagttca gaattgatta accaaacggt
     4021 agaagagtat cttcaaggag atgactttga ctgggcagat cttaaaggga aggttcgaga
     4081 taatttgacc aagtatctct ttgaccaaac caagcgtcgt ccagctattt taccagtagt
     4141 catggaagca aaataatcgt tgaaataaac agagagaaag tcgagtttcg gctttttctt
     4201 atagaaaaat agaaggagaa aatcatggca gtaatgaaaa tcgagtatta ctcacaagta
     4261 ttggatatgg agtggggggt gaatgtcctc taccctgatg ccaatcgagt ggaagaacca
     4321 gagtgtgaag atattcccgt cttgtacctt ttgcacggga tgtctggaaa tcataatagt
     4381 tggcttaagc ggaccaatgt agaacgcttg cttcgaggaa ctaatctcat cgttgttatg
     4441 cccaatacca gcaatggttg gtataccgat acccagtatg gttttgacta ctacacggct
     4501 ctagcagagg aattgccaca ggttctgaaa cgcttcttcc ctaatatgac tagcaagcgt
     4561 gaaaagacct ttatcgctgg tctttctatg ggaggctacg gctgcttcaa actggctctt
     4621 gcgacaaatc gtttttctca tgcagctagt ttttcaggtg ccctcagctt tcaaaacttt
     4681 tctcctgaaa gtcaaaatct gggaagtcca gcctactgga gaggtgtttt tggagagatt
     4741 aaagactgga caactagtcc ctattctctt gaaagtctgg ctaaaaaatc ggataaaaag
     4801 accaaacttt gggcgtggtg tggcgaacag gatttcttgt acgaagccaa taatctcgca
     4861 gtgaaaaatc tcaaaaaact aggttttgat gtgacctata gccatagcgc tggaactcac
     4921 gagtggtact actgggaaaa acaattggaa gtttttttaa caaccctacc aattgatttc
     4981 aaattagaag agagactgac ttagtttgaa cttcagcata gggggagtag aactaaaata
     5041 aaatatgttt tcactagact tttcaaacga aagtagtaga atagtaataa aatactggag
     5101 gaaagagagt aggaaatgta ccgttatcaa attggcattc ccacattaga atatgatcag
     5161 tttgtcaaag aacatgaatt agccaatgta ttacaaagta gtgcttggga ggaagttaag
     5221 tctaattggc aacatgagaa gtttggtgtt tacagggaag aaaaattact ggcgacagct
     5281 agtattttga ttagaactct tccgctaggc tataaaatgt tttacatccc aagaggacct
     5341 atattggatt atggggataa agaactcttg aattttgcca ttcagtctat taagtcctat
     5401 gctcgcagta agagagcggt ttttgtgact tttgacccaa gtatttgcct atctcaaagt
     5461 ttaatcaatc aggaaaagac agaatttcct gaaaatctgg ctattattga tagtttgcaa
     5521 caaatgggag taaggtggtc aggaaaaacg gaggaaatgg gagacaccat tcaacctcgt
     5581 attcaggcga aaatatacaa ggaaaatttt gaagaagata aactttccaa gtcaacaaaa
     5641 caggctattc gaacagcacg aaacaaaggg cttgagattc aatatggtgg actggaacta
     5701 ttagattcat tttcggagtt gatgaaaaaa actgagaagc gaaaagagat tcatttgagg
     5761 aatgaagcct attataaaaa attgttagat aattttaagg acaaggccta tatcaccttg
     5821 gccaccttgg atgtttctaa acgttcgcaa gagttagaag aacagttagc gaaaaataga
     5881 gccttggaag agacgtttac tgagtcgact cgaacttcaa aagtagaagc gcagaagaag
     5941 gaaaaagaac gtttgttaga ggaattgacc ttcttgcagg aatatataga tgtaggtcaa
     6001 gcgagagttc ctttagcggc tactttgagt ttggaatttg gtactacctc tgtcaatata
     6061 tatgctggta tggatgatga ttttaaacgt tacaatgcac caattttaac atggtatgaa
     6121 acggctcgct atgcctttga gcgaggtatg gtttggcaaa atttaggtgg tgttgaaaac
     6181 tctctcaatg gtggacttta tcattttaag gaaaaattta atccaacgat tgaagaatac
     6241 ttgggtgaat ttacaatgcc cactcatcct ctctatcctc tgttaagact tgctcttgat
     6301 ttccgtaaaa cattaagaaa aaaacataga aagtaagtat atggcactaa caacactcac
     6361 gaaagaagag tttcagactt attctgatca ggtttcttct cgttccttta tgcaatctgt
     6421 ccagatgggg gatttgctag aaaaaagagg ggctcgaatt gtttatcttg ctttgaaaca
     6481 agaaggagaa attcaagttg cagctctggt ttatagcttg cccatgctgg gtggtctgca
     6541 tatggaactc aattcggggc cgatttatac ccaacaagat gctcttccag ttttttatgc
     6601 agagttaaaa gaatatgcca agcaaaatgg tgtattagag ttgcttgtaa aaccttatga
     6661 aacttatcaa acttttgata gccaaggtaa tccaatagat gctgagaaaa aaagtattat
     6721 tcaaggtttg actgatttag gttatcaatt tgatggctta acaacaggtt acccaggtgg
     6781 agaaccagat tggttatact ataaagattt aactgaatta actgaaaaga gtttgcttaa
     6841 aagttttagc aaaaagggta aacccttggt gaaaaaggct gaaacctttg gcattcggtt
     6901 gaaaaagtta aaacgtgaag aactatcgat ttttaagaat ataacaaaag aaacctctga
     6961 acgtagagaa tatagtgata aaagtttaga atattatgag tatttttatg atacttttgg
     7021 agaacaagcg gagtttctca tagcaagctt aaatttttcg gactatatga gcaaattgca
     7081 aggtgaacaa agtaaactag aagaaaactt ggacaagttg cgacttgatt tgagtaaaaa
     7141 tcctcattct gagaaaaaac aaaatcaact gagagaatat tctagtcaat ttgaaacgtt
     7201 tgaagttcga aaagcagaag cgcgagactt gattgaaaaa tatggagaag aagatattgt
     7261 tttagctggg agtttatttg tttatatgcc tcaggaaacg acttatctct ttagtggttc
     7321 ctacactgag tttaataagt tctatgcccc tgcactgctt caaaaatatg ttatgttgga
     7381 aagcataaaa cgtggaatac ctaaatacaa cttcctaggg attcaaggga tttttgatgg
     7441 aagtgatggt gttttgcgtt ttaaacagaa ttttaatggc tatattgtac gcaaagcggg
     7501 tactttctgt taccatccat cgcctttaaa atacaaagct atccagttac tcaaaaaaat
     7561 agtaggacgt taagatgaaa aagtcagtat ttagatttct tttagcttct tttagtaaaa
     7621 taattcttat ttgctagaaa ggtggagaga catgcgctgg ctttttcgtt tgataggggc
     7681 tttcttttct tttgtgtggc gtttgttttg gcgtctggtt tggatagttg tgctcttatg
     7741 tgtgcttgct ttcggacttc tctggtatct gaacggggat tttcaaggag cgctaaagca
     7801 agcagaacgg tcagtaaaaa ttggtcaaca aagtattgac caatgggaga aaacagggca
     7861 actgcctaag ttaagccaga cagatagtca ccagcattct gaaggaaggt gggcacaggc
     7921 ctctgctcgt atttacctgg atccgcagat ggattcacgc tttcaagagg cttatttaga
     7981 agcaatccag aactggaatc aaactggtgc ttttaacttt gaactcgtga ctgagtctag
     8041 taaggcggat attacggcta cggagatgaa cgacggaggc actcctgtgg caggagaggc
     8101 ggaaagtcaa actaatctct taacagggca attcttgtcc gtaacggtgc ggttgaatca
     8161 ttattatttg tccaatccat actatggcta ctcctatgaa cgccttgtcc atacggcaga
     8221 acatgagtta ggtcatgcga ttggcttgga ccatacagat gagaagtctg tcatgcaacc
     8281 agcaggttcc ttttatggta tccaggaaga ggatgttgca aacctccgaa aaatatatga
     8341 gactagtgag tagggtacta tctttcccta ctttttttgc tataatggaa ctatgaacaa
     8401 cttgattaaa tcaaaactag agctcttgcc gaccagccct ggttgctaca ttcataagga
     8461 taaaaatggc accattatct atgtaggaaa ggctaaaaat ctgcgtaatc gagtacggtc
     8521 ctattttcgt ggaagtcatg ataccaagac agaggctctg gtgtctgaaa ttgtggattt
     8581 tgaatttatt gttacggagt ctaatattga ggcacttctc ctagaaatca acctgatcaa
     8641 ggaaaacaag cccaagtaca atatcatgct caaggatgac aagtcctatc ctttcatcaa
     8701 aatcaccaat gagcgctatc cacgcttgat tatcactcgt caggtcaaaa aggacggagg
     8761 tctttatttt ggaccctatc ccgatgtggg ggcagccaat gaaatcaagc ggttgctgga
     8821 tcggatattc ccttttcgta agtgtaccaa cccgccctct aaggtctgtt tttattacca
     8881 tatcggtcaa tgcatggccc acaccatctg taagaaggat gagatttatt tcaagtctat
     8941 ggcccaggag gtttctgatt ttctgaaagg gcaggataac aaaatcattg atgagctaaa
     9001 gggcaaaatg gcagcggcag cccagactat ggagtttgaa cgtgcggcgg aataccgtga
     9061 cctgattcag gctattggaa cgcttcgaac caagcaacgg gtcatggcta aggatctcca
     9121 aaatcgcgat gtctttggct actatgtgga taagggctgg atgtgtgtgc aggttttctt
     9181 tgtccgtcag ggtaagctca tcgagcgcga tgtcaatctc ttcccctact tcaatgatcc
     9241 agatgaggat tttttgacct atgtaggaca attctatcaa gaaaaatctc acctagttcc
     9301 caatgaggta ctgattccgc aggatattga cgaagaagct gtcaaggctt tggtggatac
     9361 caagattctc aaaccccaac gtggagagaa aaaacagctg gtcaatctag ccataaaaaa
     9421 tgcacgtgtt agtctggagc agaaatttaa cctgctagag aaatctgtcg agaagaccca
     9481 aggagctatt gaaaatctag gacgtttgct ccaaatcccg accccagtac gtatcgagtc
     9541 cttcgataac tctaatatca tgggaactag ccctgtttcg gctatggtgg tctttgtcaa
     9601 cggtaaaccg agtaagaagg attaccgtaa gtacaagata aaaacggttg ttggaccaga
     9661 cgactatgcc agcatgagag aggtcattcg cagacgctat ggtcgagtac agcgtgaggc
     9721 tttgactcct ccagatttga ttgtgattga tggggggcaa ggtcaagtca atatcgctaa
     9781 gcaggttatc caagaggaac tgggcttgga tattccaatt gctgggctgc aaaagaatga
     9841 taagcaccaa acccatgaac tgctctttgg agatccgctt gaggtggtgg acttgtctcg
     9901 caattctcag gaatttttcc tcctccaacg catccaagat gaggtgcacc gctttgccat
     9961 taccttccac cgccaactgc gctccaaaaa ttctttctca tctcaattgg atgggattga
    10021 tggtctggga cctaaacgca agcaaaatct tatgaagcat ttcaagtctt tgaccaaaat
    10081 caaggaagcc agtgtggatg agattgtcga agttggggta cctagagtcg ttgcagaggc
    10141 tgtgcaaaga aagttgaacc cgcagggaga agccttgcct caagtagcag aagaaagagt
    10201 agattaccaa acggaaggaa accacaatga accataaaat cgcaatttta tcagatgttc
    10261 atggcaatgc gacggcgcta gaagcagtga ttgcagatgc taaaaatcaa ggggccagtg
    10321 aatattggct tctgggagat atttttcttc tactcaatga aaatcaaaga gcaaactagg
    10381 aagctagccg taggttgctc aaagcacagc tttgaggttg cagataaagc tgacgtggtt
    10441 tgaagagatt ttcgaagagt gttattgtaa ctgaggttga tctgggaggt aagaaccacc
    10501 tagataggta ttgctgagtt tttcaagcgt tccgtcttga tagagttctt tgagcgcttt
    10561 atcaaatttc tctttaaact ctttttgatc acttgagaaa acgatataat tgctggggct
    10621 atctgcagaa ggtaaatcaa cgactgagag gtctaaacca cggtccttga taatcttttg
    10681 aacggatacc ttgtcaaaaa ctaggaaatc aaactctccg ttagcaaggt ctaggattcg
    10741 tttaccaata tcctcaccag aaaaatcaat tgtagcggga ttatcagtgt gtttctgatt
    10801 ccagttattg atgaattgag cgttagaagt tccggtatcc tcttgtgttg ttttaccagc
    10861 gatctggtca agagaagtca aaggattttt cttgttgctg acaaggacga ggggattgtt
    10921 ggaaatcgga agcgagtaaa ggtatttttc agcacgctct tttgtgtaac tcaaattatt
    10981 ggccgcagcc tgatagtgac cagaatcaag tcctgggaag atactttccc aggcggttct
    11041 ttgaaattga atctcgtaat cgctgagttt ttcatctact gcctttagaa cttcgatatc
    11101 gaagcctgtc agattgcctt tatcttcgta gtcaaatggt ggcacgtcgc cagctgtagc
    11161 aaggacgatt gtcttttgag cgctagtctc tttgggtgta gcttgattcc cacaggcaac
    11221 caaaaatggt aggatagcta gtaataggct aaattttttc atactgtctc cattcaaatg
    11281 taaagtactt gctagtttat cagaaacttt cctgataaac caatatatat cttttatgtc
    11341 tgtgatagaa aaacttaatc atgaaaaatc cctgcaagct ctttcaaatt atggtagaat
    11401 ggaagcagta gaattagaaa aggaaatcga ttatgaaatt tcttgaatta aataaaaaac
    11461 gtcatgcaac caagcatttc actgataagc ctgttgatcc aaaagatgtg cgtacggcta
    11521 tcgaaattgc aaccttggcg ccaagcgccc acaacagcca gccttggaaa tttgtagttg
    11581 ttcgtgagaa aaatgctgaa ctggcaaagt tggcttatgg ttcaaacttt gagcaggtat
    11641 catcagcgcc tgtaaccatt gccttgttta cagatacgga cttagccaaa cgtgctcgta
    11701 agattgcccg tgttggtggt gctaataact tttctgaaga gcaacttcaa tattttatga
    11761 aaaatctgcc agctgagttt gcccgttaca gtgagcaaca agtcagcgac tacctagctc
    11821 tcaatgcagg tttggttgcc atgaacttag ttcttgcatt gacagaccaa ggaattggtt
    11881 ctaacattat tcttggattt gacaaatcaa aagtcaatga ggttttggaa attgaagacc
    11941 gtttccgccc agaactcttg atcacagtgg gttacacaga cgacaaattg gaaccaagct
    12001 accgcttgcc agtagatgaa atcatcgaaa aaagatagga agaagaaaaa atgatagcaa
    12061 ttgattttac agcagaagta gaaaaacgca aagaagacct cttggctgac ttgtttagcc
    12121 ttttggaaat caactcagaa cgtgatgata gcaaggctga tgcccaacat ccatttggac
    12181 ctggtccagt aaaagccttg gaaaaattcc ttgaaatcgc agaccgtgat ggttatccaa
    12241 ctaaaaatgt tgataactat gcaggacatt ttgagtttgg tgatggagaa gaagttctcg
    12301 gaatctttgc ccatatggac gtggttccag ctggtagcgg ttgggataca gatccttaca
    12361 caccaactat caaagatggt cgcctttatg cgcgcggggc ttcggacgat aagggtccta
    12421 caacagcttg ttactatggt ttgaaaatca tcaaagaatt gggtcttcca acttctaaga
    12481 aagttcgctt catcgttgga acagacgaag aatcaggctg ggcagacatg gactactact
    12541 ttgagcacgt aggacttgcc aaaccagatt ttggtttctc tccagatgct gaatttccaa
    12601 tcatcaatgg tgaaaaagga aatatcacgg aatacctcca ctttgcagga gaaaatacag
    12661 gtgctgcccg tcttcacagc tttacaggtg gtttacgtga aaatatggta ccagaatcag
    12721 caacagcagt cgtttcaggt gatttggctg acttgcaagc taaactagat gcctttgttg
    12781 cagaacacaa acttagagga gaactccaag aagaagctgg caaatacaag gtgacgatca
    12841 ttggtaaatc agcccacggt gctatgcctg cttcaggtgt caatggtgcg acttacctag
    12901 ccctcttcct tagccagttt gactttgctg gtccagccaa agaatacctt gacatcgctg
    12961 gtaaaattct cttgaacgac catgagggtg aaagtctcaa gattgctcat gtggatgaaa
    13021 agatgggtgc cctttctatg aatgcaggcg tcttccgctt tgatgaaaca agcgctgata
    13081 ataccattgc cctcaacatc cgctatccaa aaggaacaag tccagaacaa atcaagtcaa
    13141 tccttgaaaa cttgccagtt gtttctgtta gcctttctga acacggtcac acgcctcact
    13201 atgtgccaat ggaagatcca cttgtgcaaa ccttgttgaa tgtctatgaa aaacaaacag
    13261 gccttaaagg tcatgaacaa gtcatcggtg gtggaacctt tggtcgcttg ttagagcgcg
    13321 gagttgccta tggtgctatg ttcccagact caattgatac catgcaccaa gccaatgaat
    13381 ttattgcctt ggacgatctc ttccgagcag cagcaattta tgccgaagct atttacgaat
    13441 tgatcaaata aaacgataga agtctgagat cttatgcttg gacttctttt tggagggaaa
    13501 gtagatgtct caaatcgaaa gaatcaaaca ggctatcatg gcggattcgc agaatgccag
    13561 ctatacagag cgtggcattg agcctctctt tgcagcgcca aaaattgctc gcatcaatat
    13621 catcggtcag gctccgggac ttaaaactca agaagcaggc ctttactgga aagataaaag
    13681 tggtgaccgc ttgcgggact ggctaggtgt ggatgaagat accttttaca attcaggtta
    13741 ttttgctgtt ttgcctatgg atttctactt tccaggacat ggcaagtcgg gtgatcttcc
    13801 gcctcgtaca ggttttgcag aaaaatggca tccgcagctc ttacaggaat tgcctgatat
    13861 tcagttaacc ctcttgattg ggcaatatgc ccaagcctac tatttacagg agaaaatcag
    13921 tgggaaggta acggagaggg tgaaacacta taaagactat ctgccagcct attttccgct
    13981 agttcaccca tcaccacgaa atcaaatctg gatggtcaaa aatctttggt ttgaggcaga
    14041 agtagtgcca gatttgaaaa aaagaattaa aaccatttta tagtcaatga aaatcaaaga
    14101 gcaaactagg aagctagtcg taggctgttc aaagtacagc tttgaagttg cagataaaac
    14161 tgacgaagtc ggtaacatac gcacggtcag gcgacgctga cgtggtttga agagattttc
    14221 gaagagtatt agaagaaaaa gaatgaaaga aatagccttt gacgcatttt accagcttta
    14281 ccaaaacgac cagctttctc tagtggatgt gagagaagtg gatgagtttg cagctcttca
    14341 tttagaaggt gcccacaacc taccgcttag tcaattggct gatagttatg attaattgga
    14401 caaagatcgc ttgcattata ttatttgcaa atctggaatg agatcggcgc gtgcttgcca
    14461 attcctatta gaacaaggtt ataatgttat caatgtccag ggtggcatgt tagcctttga
    14521 agaactttaa aattttacat ttctcctact tggtgtggac tgggtaggag agttttattt
    14581 ttagataatt cttattttta agaaaattga aaacatttaa tatttgcctc gtgatgcttt
    14641 tttcagactc ctaatcgtgg tatactaggt cagtatttta taaatatgaa ggagattttt
    14701 atggctaaaa aaggtaccct aacaggtttg ctcctgtttg gaatattttt tggtgcgggg
    14761 aacttgattt ttccgccttc tctaggtgct ctatctggag aacattttct tcctgccatc
    14821 gcaggttttg tcttttcagg cgttggtatc gccgtcttga cccttattat tggaacgcta
    14881 aatcctaaag gatatatcta cgagatttca acgaagatag cgccttggtt tgcgactctt
    14941 tacctctcag ttctttactt gtcaatcggt ccattctttg ctaccccacg tactgctaca
    15001 acagcttacg aagtagggat tagccccctt ttgtcggatg caaataaagg acttggcttg
    15061 attgtattta cggttctgta ttttgcggca gcctatttga tttcacttaa tccatcaaaa
    15121 atcttagacc gcattggacg tattttaacg ccagtctttg caattttgat tgttatcttg
    15181 gttgttctgg gagctatcaa atatggtgga acaagtcctc aagctgcttc agctgcttat
    15241 caagcttctg cctttggtac aggtttccta gaaggttaca ataccttgga cgcccttgcc
    15301 tcagtggcct ttagcgtaat cgcagttcaa accttgaaac aacttggatt ttcaagtaag
    15361 aaagaataca tttcaactat ttgggttgtt ggtatcgttg ttgcccttgc cttcagcgct
    15421 ctttacatcg gtttaggttt tcttggaaat catttcccag taccagctga agcgatgaag
    15481 ggtggaacac caggtgttta catcttgtca caagccactc aagaaatctt tggctcaaca
    15541 gctcaactct tccttgcagc tatggttacc gtaacttgct tcacaacgac tgttggtttg
    15601 attgtgtcaa cagctgagtt ctttaatgag cgcttcccac aaatcagcta caaggtttat
    15661 gcgacagcct ttaccttgat tggatttgct attgccaatt tgggtcttga tgcgattatc
    15721 aagtactcaa ttccagtact ggttatcttg tacccaatca cgattgctat cgttatgatt
    15781 gtcattgtca acaaatttgt tgccctttca aaaccaggta tgcagttgac aattgctgtg
    15841 gttacagtta ttgccattgc aagcgtacta ggaagctcgt ttaaggttga gtttcttgca
    15901 aatcttgtta gcgttcttcc ttttgccaag gcatctctcc catggttggt gccagccatt
    15961 gttggaatct tgctctcatt ggttctacca aacaagcaag aaagtgatgt ttttgaaatg
    16021 gaataatcac taatactctt cgaaaatcaa attcaaaccg cgtcaacgtc gccttgtcgt
    16081 actcaagtac agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtataaa
    16141 atcacttttg tagtcaagtc tacaggagtg attttctttt tttatccgat gataaatgtg
    16201 ttataatagg tagcaaaaga ggtgaagaaa tgaatcaaac agtagaatat atcaaagaac
    16261 tgacagccat tgcgtcgcca acaggcttta ctcgtgagat tgcggactat ttagtcaaga
    16321 ctctagaagg ttttggttac cagccagttc gcacagctaa gggtggtgtc aatgtgacca
    16381 tcaaaggtca aaatgatgag caacatcgct atgtgactgc tcatgtggat acgctgggtg
    16441 ctattgttcg tactgtcaaa ccagatggtc gtcttaaatt ggaccgtatc ggtggttttc
    16501 cttggaacat gattgaaggt gaaaactgta ccgttcatgt ggctagcaca ggtcaaaagg
    16561 tatcaggaac catcctcatc caccaaactt cttgccatgt ctacaaggat gcaggaactg
    16621 cagagcgcac gcaggacaat atggaagtgc gtttggatgt aaaagtaagc aatgaaaaag
    16681 aaactcgtgc tcttgggatt gaggtcggtg attttatcag cttcgatcca cgaactgttg
    16741 tgactgatac aggttttatc aagtctcgtc atttggatga taaggtcagc gcagcgattt
    16801 tgctcaacct gcttcgtatt tataagaaag agaagattga attgccagta acaactcatt
    16861 ttgctttttc agtttttgaa gaagtaggac atggtgctaa ttctaacatt cctgctcagg
    16921 tagtagaata tctggctgtg gatatgggag ctatgggaga tgaccagcag acagatgagt
    16981 acacagtatc tatctgtgtc aaggatgctt caggtcctta tcactatgac ttccgtcaac
    17041 acttggttgc cttggctaaa gagcaagata ttccttttaa gctggacatc tatccatttt
    17101 atggttcgga tgcttcagcg gctatgtctg caggggcaga agttaaacac gctcttcttg
    17161 gtgcgggtat cgagtctagt cattcttatg agcgtactca tattgactcg gtggtcgcaa
    17221 cagagcgtat ggttgatgct tatcttaaga gtgggttggt agactaatat gtgcctgatt
    17281 tgccagagaa ttgacctcat caagaaggaa gaaaatcctt actttgtcaa agagttggaa
    17341 acaggctatc ttgtgattgg agaccaccag tattttgcag gatatagtct ttttctagct
    17401 aaggaacacg ttaccgaatt gcaccatttg aaaaaggaaa caagactccg ttttctcgaa
    17461 gaaatgagtc tagtccaaga ggcagttgcc aagaccttta ctgctgagaa aatgaatatt
    17521 gaacttttag gaaatggcga tgcccatctc cattggcatc tgtttcctag acgaagaggt
    17581 gatatgaagg gtcacggtct caaaggacgt ggtccagtat ggtgggttcc ctttgaagaa
    17641 atgacctcag aaacctgcca agcaaaaccg gatgagatta aaagattagt caaacgttta
    17701 tcgtcagaag tagataaact attagaataa aggagtagaa atgaaaaaaa gatacctcat
    17761 cttgacagct ttgctagcct tgagtctagc agcttgttca caagaaaaat caaaaaatga
    17821 agacggagaa gctaagacag aacagacagc caaagctgat gggacagtcg gcagtaagtc
    17881 tcaaggagct gcccagaaga aagcagaagt ggtcaataaa ggagactact acagcattca
    17941 agggaaatac gatgaaatca tcgtagccaa caaacactat ccattgtcta aagactataa
    18001 tccaggggaa aatccaacag ccaaggctga gttggttaaa ctcatcaaag tgatgcaaga
    18061 ggcaggtttc ccaatcagtg accattacag tggctttaga agttatgaaa ctcagaccaa
    18121 actctaccaa gattatgtca atcaagatgg aaaggcagca gctgaccgtt actctgcccg
    18181 tcctggctat agcgaacacc aaacaggctt ggcctttgat gtgattggga ctgatggtga
    18241 tttggtgaca gaagaaaaag cagcccaatg gcttttggac catgcggctg actatggttt
    18301 tgttgtccgt tatctcaaag gcaaggaaaa ggaaacaggc tatatggcag aagaatggca
    18361 ccttcgttat gtcggaaaag aagctaaaga aattgctgag agtggtctca gtttggaaga
    18421 atactatgga tttgaaggcg gagattacgt cgattaatac tcttcgaaaa tctcttcaaa
    18481 ccacgtcagc gtcgtcttac ctactgactg cgtcggttct attcacaacc tcaaaacagt
    18541 gttttgagct gacttcgtca gttttatctg caacctcaaa gctgtacttt gagcagcctg
    18601 cggctagctt cctagtttgc tctttgattt tcattgagta taaaaaataa acttttctct
    18661 tgcaattcca gataaatagt gtataataga tgggtatgtg aaaagcatac ttgtgggagg
    18721 taaaaatctc taattaccgc caaaaccaca aaggaggatt taaaaatggc taaaaaagtc
    18781 gaaaaacttg taaaattgca aatccctgct ggtaaagcta caccagctcc accggttgga
    18841 cctgctcttg gtcaagctgg tatcaacatc atgggattca caaaagagtt caacgctcgt
    18901 acagctgacc aagctggtat gatcattcca gttgttatct cagtttacga agataaatca
    18961 tttactttca tcactaaaac accaccagct gctgttcttt tgaaaaaagc tgcaggtgtt
    19021 gaaaaaggat caggtacacc taacaaaact aaagttgcta cagttactcg tgcgcaagta
    19081 caagaaattg cagaaactaa aatgccagat ttgaacgcag caaacgtaga gtctgcaatg
    19141 cgtatgatcg aaggtactgc tcgttctatg ggattcactg ttgttgacta atcaataaca
    19201 cagtagaaaa tctcacagca gtctattagt tgcttttcat actaggcaag tgactgatgc
    19261 ttgtacttgg gtacagcaag ggagcttaag gccgtaggaa aagaatctaa tagactgaaa
    19321 acccgcaaga cttcatcatt tcgagaagtg acgtgggaga tgaaaatcga ttgaaccact
    19381 tacaaggaga atagaaaatg gctaaaaaaa gcaaacaact tcgtgctgct cttgagaaaa
    19441 tcgacagcac aaaagcatac agcgtagaag aagctgtagc acttgcaaaa gaaacaaact
    19501 ttgcaaaatt tgacgcaact gtagaagttg cttacaactt gaacatcgac gttaaaaaag
    19561 ctgatcaaca aatccgtgga gcaatggtat tgccaaacgg tactggtaaa acttcacgcg
    19621 ttcttgtttt cgcacgtggt gcaaaagctg aagaagcaaa agctgctggt gcagactttg
    19681 ttggtgaaga tgaccttgtt gctaaaatca acgacggttg gttggacttc gacgtagtta
    19741 tcgctacacc tgatatgatg gctcttgttg gacgtcttgg acgtgtcctt ggaccacgta
    19801 acttgatgcc aaaccctaaa actggtactg taacaatgga tgttgctaaa gcagttgaag
    19861 agtctaaagg tggtaaaatc acttaccgtg ctgaccgtgc aggtaacgtt caagcaatca
    19921 tcggtaaagt atcatttgaa gctgaaaaat tggttgaaaa ctttaaagct ttcaacgaaa
    19981 caatccaaaa agcaaaacca gctacagcta aaggaactta cgtaacaaac ttgactatca
    20041 caactactca aggtgttggt atcaaagttg acgtaaactc actttaatca gtagttaaag
    20101 taatgtaaaa aagttgaaga cgctatgtct caactttttt tgatgtacga cgggcatgtc
    20161 gtatatctga ggtgtaagtc ctcggtgggc acctgctacc ggtgaaccca atagcgattc
    20221 ccaagtctga cgcaggaaat aagaatcgtc agaggaaggg atagcgaaat cgtggctcta
    20281 cgaacaggaa catgataata aggcctatat agcggataag gtatctggct ggggcgacca
    20341 ctatcaatta gtatctcaga agtcggtact taaacgtgct atatcaaaac cagtccttga
    20401 aaaacgtgga ctggtttcgt gtttggatta ttaccttgaa tgacatgcgt taacagttag
    20461 ttgaaccgcc gtatgccgaa cggcacgtac ggtggtgtga gaggggctag agattatccc
    20521 ctactcgatt tcgaaatcta gtggaatgaa tctggaatag tccatcgagc tttctaatac
    20581 tcttcgaaaa tctcttcaaa ccacgtcaac gtcgccttgc cgtgcgtatg gttactgact
    20641 tcgtcagttc tatccacagc ctcaaaacag tgttttgagc aacctgagac tagtttccta
    20701 gtttgctctt tggttttcat tgagtataac acattgttag aagttggttt aaatttccta
    20761 atcagtttgt tcacatttac cttcgatata ttatatccca tagttaaggt tggtcataca
    20821 gatgattata gtcatggagc cgtaaaactt agtgtttctt tagttgacaa agatgccatg
    20881 aaaaaaatat ctgtaactgt aataggatat tttgaaacaa atatagatga aaatatcacc
    20941 gatattctat acgtaaatgg tactgctatt ctttatcctt atttacgttc tattgtttca
    21001 atagtttcgg caattgatag cagtgaagca atgttgctac ctaccattaa tgttttagag
    21061 ttactagata aatctcaacc ttttgaagaa gaataattta ttagctcact aaattgaggg
    21121 taaggaaaag taaaagcagt aagaaaaatg tcttgcatta tacagcaacc ttttgggaat
    21181 gagtagatgg attgaataaa atttgattaa gagtggatga tttatctgta gattattatt
    21241 ggacagttag tcttgaagta gtctaagaat taggttataa tcagtagaag ccttgctaat
    21301 aatgaggagg ttagtttat
//
LOCUS       DAVKJU010000034        20736 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVKJU010000034 DAVKJU010000000
VERSION     DAVKJU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20736
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.034"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(122..271)
                     /locus_tag="VW191_001207"
                     /pseudo
     CDS             complement(122..271)
                     /locus_tag="VW191_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(425..2056)
                     /gene="rlmD"
                     /locus_tag="VW191_001208"
     CDS             complement(425..2056)
                     /gene="rlmD"
                     /locus_tag="VW191_001208"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9623979.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKIFMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2130..3104)
                     /locus_tag="VW191_001209"
     CDS             complement(2130..3104)
                     /locus_tag="VW191_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEU9623980.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3178..3792)
                     /gene="pmp23"
                     /locus_tag="VW191_001210"
     CDS             complement(3178..3792)
                     /gene="pmp23"
                     /locus_tag="VW191_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEU9623981.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3792..4766)
                     /locus_tag="VW191_001211"
     CDS             complement(3792..4766)
                     /locus_tag="VW191_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEU9623982.1"
                     /translation="MDIYIKKAIIHQFSLDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4774..6030)
                     /locus_tag="VW191_001212"
     CDS             complement(4774..6030)
                     /locus_tag="VW191_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU9623983.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(6092..6523)
                     /locus_tag="VW191_001213"
     CDS             complement(6092..6523)
                     /locus_tag="VW191_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623984.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6525..7124)
                     /locus_tag="VW191_001214"
     CDS             complement(6525..7124)
                     /locus_tag="VW191_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEU9623985.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7111..7950)
                     /gene="prmC"
                     /locus_tag="VW191_001215"
     CDS             complement(7111..7950)
                     /gene="prmC"
                     /locus_tag="VW191_001215"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU9623986.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALDLASENAKNQNLQ
                     IFFKKSDCFTEISEKYDIIVSNPPYISREDESEVGLNVLYSEPHLALFADEDGLAIYR
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(7950..9029)
                     /gene="prfA"
                     /locus_tag="VW191_001216"
     CDS             complement(7950..9029)
                     /gene="prfA"
                     /locus_tag="VW191_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU9623987.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLKELNK"
     gene            complement(9039..9518)
                     /locus_tag="VW191_001217"
     CDS             complement(9039..9518)
                     /locus_tag="VW191_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9623988.1"
                     /translation="MTIELRDVTMENYFDVLNLDVKEYQKQFIATNAISLAEAYVYTK
                     NGDFVAPLAVYDNDVIIGFVMIAYDKKIVISSGNYLLFCFMIDKNFQNQGYFKPIMDK
                     VLDYVRTAPAGLSNKLWLSYEPENEQARFCYLSYGFKETGEISENEVVAIYDLTIEK"
     gene            complement(9508..10107)
                     /locus_tag="VW191_001218"
     CDS             complement(9508..10107)
                     /locus_tag="VW191_001218"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU9623989.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPMITSNKEQNYDN"
     gene            10230..10412
                     /locus_tag="VW191_001219"
     CDS             10230..10412
                     /locus_tag="VW191_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEU9623990.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10549..11922
                     /gene="mnmE"
                     /locus_tag="VW191_001220"
     CDS             10549..11922
                     /gene="mnmE"
                     /locus_tag="VW191_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU9623991.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITKLF
                     SQFCLGK"
     gene            complement(11969..12904)
                     /gene="dapA"
                     /locus_tag="VW191_001221"
     CDS             complement(11969..12904)
                     /gene="dapA"
                     /locus_tag="VW191_001221"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU9623992.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGFINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12960..14036)
                     /locus_tag="VW191_001222"
     CDS             complement(12960..14036)
                     /locus_tag="VW191_001222"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU9623993.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14378..14450)
                     /locus_tag="VW191_001223"
     tRNA            complement(14378..14450)
                     /locus_tag="VW191_001223"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14415..14417),aa:Thr,seq:ggt)
     gene            complement(14550..15059)
                     /locus_tag="VW191_001224"
     CDS             complement(14550..15059)
                     /locus_tag="VW191_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEU9623994.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLIDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15057..15153)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15182..15613
                     /locus_tag="VW191_001225"
     CDS             15182..15613
                     /locus_tag="VW191_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU9623995.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15695..16072
                     /gene="mscL"
                     /locus_tag="VW191_001226"
     CDS             15695..16072
                     /gene="mscL"
                     /locus_tag="VW191_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU9623996.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16114..17208)
                     /gene="hemH"
                     /locus_tag="VW191_001227"
     CDS             complement(16114..17208)
                     /gene="hemH"
                     /locus_tag="VW191_001227"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU9623997.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17362..18585
                     /gene="pepT"
                     /locus_tag="VW191_001228"
     CDS             17362..18585
                     /gene="pepT"
                     /locus_tag="VW191_001228"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEU9623998.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18621..19250)
                     /locus_tag="VW191_001229"
     CDS             complement(18621..19250)
                     /locus_tag="VW191_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9623999.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            19215..19436
                     /locus_tag="VW191_001230"
     CDS             19215..19436
                     /locus_tag="VW191_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624000.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNIL"
     gene            complement(19707..20719)
                     /locus_tag="VW191_001231"
                     /pseudo
     CDS             complement(19707..20719)
                     /locus_tag="VW191_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6009 a         4340 c         3873 g         6514 t
ORIGIN      
        1 aatggatact ataaacagca caccaactat ttattttttg agttttaaac tttggtagaa
       61 gtcttgaact ccttatattt acataactat ttgcctctat aactattatg acacgaaacc
      121 aacaagcaag ttcaagcacc tccatatctt tattcaaatg aggactaaga tattcacaaa
      181 ctttatcata aacctcttta tcttttttca taaaaggggc atacaactta gcaaatttat
      241 cctaaaattt tttgttgcct ttatcaatca tttcaacact cctttaaata taaaaatgat
      301 cctatcaatc tttatattga gcctattttc taattgatac cttgcaagaa aggaataata
      361 gctgttccta taccaagtag gaatgtaata aatgcagaaa gtcatatact gtcattcttt
      421 tctattaaat cattttgaag tgtctcaaag catccatgat ggcttcttct ttttcaggtg
      481 tacactgtgg aataatttgt ttatcctttt tagacttgtt gtaatgttct cgtaattcta
      541 ttccacattt cttttttatc tgtgcaatat ataatgtcga aacttttaat tcaaatttat
      601 tccaaacata ttctttgatt tgagcatatg ttgctttgct ctccgcactt gtcaaatcca
      661 tctcatccag ctcaatttca acacttatgt gcttatcgac atcaagtttg gacaaaagtg
      721 ctaccgtctc gacgtgatgc gtttgaggaa atagatccac cggctggact ttcttcaatt
      781 catatcccaa ttcttggtag agtttgatat cacgcgccat ggttgcgaca ttgcaggaga
      841 tataggcgat gcgatcggct cctgtttggg cgcttgcttt gataaagctt tctgtcaagc
      901 ccttgcgtgg aggatcaacc aagataacgg ttggttgaat cccatcttta agccaattct
      961 tcatagcatt ttcagctgtg tcacagacat agtgggcgtt tgaaatattg ttcagctgag
     1021 catttttttt actattctca accgcttctg gaatcagttc aacaccgtag acttctttga
     1081 catgcttggc gactgataaa ccaatggttc caataccaga ataagcatca ataaccacgt
     1141 catctttttt taactctgca aagtcaatgg ctgtttgata gagtttctcc gccatttcag
     1201 tattgacttg gtaaaaggct gggccagcga tttggaagtc atttcccaac atctggtccg
     1261 taatatagtc ttgaccataa agagtgcgcc actccttacc aaaaatcgca ttggtattct
     1321 ggtcgttgat attttgcatg acagacacaa tctctgggaa ctgcttgata acttgttcaa
     1381 tcaattggtc aacacgaaaa acttttggac gagttgtcac caaaacgacc atgatttgtc
     1441 ctgaatagtg accacgacgc accacaagat tccgaatcaa tccagactgt tccttttcgt
     1501 cataaggttt taaatcaaaa cgacggagca ggtctcgaag agctactacg acttggtcaa
     1561 tgacaggatc ctggataaag aaatcttcaa ggggcatgag gttatgcgaa ttcttacgga
     1621 aaaatcctgt ttccaagaca ccattcactc gacgaacggg cacctgcgcc ttattgcgat
     1681 acttgactgg atgttccata ccaagcgttt cagcaacttc tacatctgca attccagcaa
     1741 tcttgtagag actgtccttg acttgcttgg ttttaaactt gagctgttct ggataagaaa
     1801 ggtgtcctaa atccgcgatt cctgaacgca ggtaagccaa atctagatct tgattacggt
     1861 gtggtgactg gacaaggtat ttttcaactt ttccaaagcc aatcttttta ttgaccttga
     1921 ggacacgcat gaaaattttt tcactcggta aagcattctc tacaaaaaag accaaaccat
     1981 ctaccttggc aactcctgcc ccttcatggg tcaaatcaac aatttcaact tctacaatat
     2041 catttttctt taacattctt ttctctttct attggccgac aaaaaagacg gcaacgttac
     2101 ggttaccatc cattatacca tgaaatttct taagaaccaa aactcttgaa gaagttgaca
     2161 agggcttgcc agagggagct taagaagtta ccaccgtcct ctagcgcctt atccgcatca
     2221 aagttaaggt tgatattttt aaaactgtcg ccagcttgtg atacgatgct ttgtttaagg
     2281 tcatttaggg ttttagtgaa atctgcattg ctgaggatat cactctttga gagattcaag
     2341 gcaaaattga tgatgatatt gatctggttt cctgttatga cctgatcaag tttgtaattt
     2401 tttaaggtat cttcaacaat cttgcggata tcttcttctg tcagatttcc cttgctttct
     2461 ttagctttgg cgagtcctga cttgatatca gctagggcaa cgtttaattt attagcatca
     2521 tagcctgatt tgtccttgtt ttcagcattg atatctgaca aagcctttag ctcttcttga
     2581 gccaaatctt tattggcttg cggcaccttg gctccattag cttctagcga atagtagatc
     2641 ccagccaaag cactctcacc tgtaactgga atgggtgctg ctacagtgat tttggcgtgt
     2701 tccacaccca aagttacggc tgcatttcgg tacatatcct gagttacctt agtgatgttt
     2761 tcaggtgttt caatcttgac ctcaagtggc gatttgtcac ctagcttttg aatcttggct
     2821 gatgaataca actgtaagct agagtcattg gccacattca tgattttaga ataaacatca
     2881 ggtgtcatgg tcttgagttc tttggtatct gttgaggcat tgtagcccag ttttttaaga
     2941 gtttgatttt tttggtcttc agatagggaa gaacctagga catattcagg ttggacatag
     3001 gtttcatcga taactttttg aacatctgtt gctgcatgga cgctattcat agctgttact
     3061 gcccacaaga tcgcagcgct agtcagaaag agtttctttc tcatagggaa tttcctcctt
     3121 tacttcttta gagtaatata tctatcttaa agaaaactta taacaaaaac acctggtcta
     3181 gccagatgtt gaaaagagag tgaaacattt gatgatgtaa aggttaagtc gtacctgtct
     3241 agaataataa tagtttcctc catttacata gagttcagca ccgtgaaaaa tggaaatggg
     3301 gtgaatataa ctataagtct ttccagtcgt attaccaagc aagggggcaa cagtctcacg
     3361 agagtactgt ttggctagag ccaaggtatt ttccttgcca ttttgggcga taaaatcgat
     3421 ataggcaggt ccaaaattat aggcttgaac agctgtccag atatctacac ccttcttctg
     3481 cgccagatag agattgcctg tcagagtttg aatgccttgc cgaatgctag aggcattatc
     3541 attgatggtg ttggtggaac cacttgcaga ctcactagac tgcataacat cgccttcttt
     3601 tccttttgtt tcagtataaa tcatagcaag cacaagctct tcgtttgctg gggtgtcttt
     3661 ttcactcaat atttctcgca ccatgggttg ataggtcatg acttgtttga catcttgatg
     3721 aacgcggtaa gctttatagc cagcaaaaag gaagactgct agtacaagca ctcttcgaat
     3781 tcgtttaaac atcatttact ttggatatcc tcgatatttt tgattaagat agagtaggtt
     3841 ccattttcgt tttggataaa ctcaacagac tcggcgtctt gatagacgtt attgggaacg
     3901 atgagctcaa ttccatttga taaggagagt ttttggtttt caaatttctt taattggcga
     3961 ctggcatcaa tttcatcaaa ttgaacaggt tctggtacgg cttctttgac ttggtcaata
     4021 aagctcaaac gagccgtcag attgttgtca aaaaggtcat tagccaattt ctcaggtgac
     4081 aattcattgc tttcttctag gttgttgaaa atagctgatt tgaccttgga ttgaaattga
     4141 aaatcatctg tgttaaaaga ttcagcaatt ctctgggctg ttttttccag ttccttgata
     4201 gattttttag gagaaatctt aggagcgaca gcaagaagat tatctgaaaa atagttcaaa
     4261 aaagtcccgt tgtacttgat tcgtttttca atcaggtgat acttgcgact ctgaagattg
     4321 accaccaagg cctcatcagc tcccgttcca aatccaggca ggttattctg agtcagcttg
     4381 attggattat caacttctcc tccgaggtgg gtcaaggtct cccgcagggc aattcgcaag
     4441 aaagcgaaat gttctacacc ttctttagaa aattgaacaa aaatcaagtc attggtcttg
     4501 agattttcag aaatgctaaa ctcctctttc cagagattag ccagcgttac tgatgtctcc
     4561 aacaaatcgt ctgtaatatg attgaagaag ggattttctt cttcgaaaat cccagtcttg
     4621 gcttcatctg aatacacatg ttcaattttt ttacgtaggt attcttcgat ttttggagta
     4681 atattgagaa acttatctgc taagaacagc tcggtatcat ccagactgaa ctggtgaata
     4741 atggctttct taatataaat atccataaaa gttttagtcc tcgtataatg ggaaggcatc
     4801 tgtcaattct ttgactgcac ttctcacttc ttctaataca gcctcatttt ctgaattctt
     4861 aagggtttta atgatgagtt cagccacttt gcgactttct tcttcaccaa atccacgtgc
     4921 agtaatagct gctgctccga tacgaatccc acttgtcttg aatggcgaca aggtttcgta
     4981 agggattgaa tttttattta gggtaatatt gacttcatcc agcaagtttt gagcaacttt
     5041 tccgttttct acaaccttag tcacatcaac aaggaagaga tggttttcag ttccacctga
     5101 aataatacgg aaatcagggt cttgcaagaa gacatctgcc atagccttgc tgttcttaat
     5161 tacattggca gcatattcct tgaaggctgg atccaaaact tctttgaagg aaactgcctt
     5221 agccgccaca acatgctcta aaggaccgcc ctgaatacct gggaaaatag ctgaattgat
     5281 ttttttagca agttcttcgt cattggtcaa aatcaaacca ccacgaggtc cacgaagggt
     5341 tttgtgggtc gttgttgttg tgatatgagc gtatggaact gggcttggat gaaggccagc
     5401 cgcaaccaaa ccagcgatat gggccatgtc caccatgagc ttcgcaccga cagcatctgc
     5461 gatttcacgg aattttgaaa aatcgataat ttgagaatat gctgaagcac cagctacaat
     5521 cagttttggt tttacttctt gggcttgttt caagatagca tcaaagtcta agagttccgt
     5581 ttcaggatca acactataag aaacaaagtt gtaggtttga ccagagaagc taacaggagc
     5641 cccatgagtc aaatgaccac ctgatgccaa atccattccc ataaccgtat cacctggctc
     5701 aatcaaggac atgtaagccg cacagttagc ttggcttcct gaatgtggtt ggacattggc
     5761 aaatttagca ccgaaaattt cttttgcgcg ttcaatagca agagtctcta caacgtctac
     5821 tacatcagtt ccaccataat aacggcgtcc tgggtaaccc tcggcatatt tatttgtcaa
     5881 gatagaccct tgagctgcca taacagcctt ggaaactacg ttttccgaag caattaactc
     5941 gatattattt tgttggcgtt cttcttcttt ggcaatagca ttccagagat cagcatcata
     6001 tgctttaaaa tcatctttgt caaaaatcat aggtcttctc ctttattgtg tgactagtcc
     6061 attagtttga ttttacaata agaaaatcaa actaaaagat gcgaataaac cgtttctgca
     6121 ttttatcaca agtatagcca actttttcat aaaatgcatg agcacccaga cgatgattgg
     6181 cagaatttaa gcggataaac ccataaccac atcttttggc ttcttgttcc aacccttgta
     6241 gtaaactttt accgatacct tgaccttgcg cttgaggtga aactgctaaa gctaagatat
     6301 taaatcctgc tttggaatag agtgattcgt aaacttcagc gtggacatat ccaagtaaga
     6361 catgattagc tgcatcctca tagccaagta ggaaatgatg ggaatcctga gacagtctag
     6421 ctagttggct agccgtttcc tctggactaa aagtataacc caaagcctct tggttgatgt
     6481 cacatatcgc tttcacatct gtttcttgca aatctcttag catttcaagc ctcctcaaaa
     6541 gaaatctctg gcaaccgagc aagaatatct tctcgtttaa ttgcgccttg gcgtaagatt
     6601 ttcaccttgt ctccagacaa atccacaata gttgaatcct gtccagttag aaaagcatcg
     6661 tcttccagac ccagaacctc ttggtcaaaa tccttcagaa tttgttcaaa ggttacacca
     6721 cttgcctgac ctgagatatt ggcagacggc ccaatcaagg gacctgtctc tcgaattaaa
     6781 tccagtgtga tagggtgact gggcatccga aatccaatag ttgcaaggtc agaatttacc
     6841 caatagggaa ctcggtcatt ggcttcgaga ataatggtca agggacctgg caaaaaggtc
     6901 tctacaagtt tttgtagata agctggctga ttcttagaaa agtgcaagat atcctctaaa
     6961 gaggcaacat tgagattgag cgccttgtct ctaggacgac gtttgagctg gtaaacatgg
     7021 tcaactgctt tttcgtctaa ggccttggca aaaagaccat aaaccgtctc tgtaggcaga
     7081 atgacagctc caccattttc caactcttgt ctaatcctgt ccatcatcaa ccacgaccat
     7141 cctatcttga ccaaattggt ccttgagtgt tcgaactttt ttttcaggaa gatgttttct
     7201 aaaaagttcg ggaacacttt gaccttgctt gtatccaatt tcaaggtaaa tcttaccacc
     7261 atctttgaga tagtcttttg catcttccgc aattctacgg taaatagcta ggccatcctc
     7321 gtctgcaaag agagctagat gaggctccga atacaagaca ttcaagccta cctctgactc
     7381 atcttcacga gagatatagg gtggattgga aacaattata tcatattttt cagaaatttc
     7441 tgtaaaacag tcagattttt taaaaaatat ttgaagattt tgatttttag cattttcgct
     7501 agctaaatct aaagcctctt gagaaacatc tgctgctgtc actgaccaat ctggtctatt
     7561 ttttgctaga gcgagagcaa tagctccact acctgttcca atatccagaa ctgaaagatt
     7621 cgtctcaaga ttttcagtta gaataagctc cactaactcc tctgtttctg gacgaggaat
     7681 caaaacccgt tcatccacct ttaaatgcat tccataaaaa tctgcctgtc caatgatgta
     7741 ctgagctggc ttgtgagctg ctagttgctg gtaaatatct tctacaaatt gtttttcttc
     7801 ctctgttgtc acctcctgct ggagggcaaa aataaagtct gtaaaagata gatttttcag
     7861 actacgatag acaaaagaga ggctttccgc ttcctctcct tgtcttatca actcttcttc
     7921 aaaatttgaa aataattgag ctaatttcat tatttgttta attcttttag tttttgtgtt
     7981 tggtcataaa gcaccaaggc atccacaact tcgtccaatt taccagacaa aatcgtatct
     8041 agtttttgga gggtcaagcc gatacggtgg tctgtgacac ggttttgtgg gaagttataa
     8101 gttcggatcc gttctgaacg gtcaccagta ccgattgtcg acttacgctc agcgtcttgt
     8161 tcatcctgag caatctgagc aaagtggtca gcgacgcgtg cacggataat cttcatagcc
     8221 ttctcgcggt tcttctgctg ggtacgttct tcctgcatct caaccttgat attggttggc
     8281 aagtgaacga tacgaacggc agtcgcaacc ttattgacgt tctgtccacc agcaccagag
     8341 gcgtgataga tgtcgacacg aaggtctttt ggatcaatgt cgtattcaac ctcttcaact
     8401 tctggcataa caagaactgt cgctgtcgaa gtatgaacac ggccttggct ttctgtcaca
     8461 ggaacacgtt gcacacggtg ggcacctgat tcatacttaa gcttagagta tacagactga
     8521 cctgaaacca tagcaaccac ttctttaaaa ccaccgacac cattcataga ggcttccatg
     8581 acttcaaagc gccaaccttg ggcttccgca tacttttggt acatagttag caaatctcca
     8641 gcgaaaagtg ccgcttcgtc tccaccagct gctccacgga tttcaaggat gatattcttg
     8701 tcatcgtttg gatcctttgg aaggagcaaa attttcagtt tttcttcata ttcttctttt
     8761 tcagccttgg catctttgag ttcttgcttg gccatttctt ccaagtccgc atctccgcct
     8821 gattccttaa tcatctcttc ggcatcgacg atattttgaa ggacttgttt atactcacgg
     8881 taggctatta cggtgtcacg attggaagct tcttcttttg aaagctccat aaaacgcttg
     8941 gtgtctgaaa cgacatcagg gtcactcagc aattctccta gttcttcata acggtcttct
     9001 acagcttgta gttgatcata gatgttcatt ttttctcctt atttctcaat tgttaaatca
     9061 tagattgcta ctacttcatt ctcggatatt tccccagttt ctttaaatcc ataactgaga
     9121 taacaaaatc ttgcctgttc attttctggt tcataagaca accaaagttt attgcttaaa
     9181 cctgctggcg ctgttcgaac atagtctagt actttatcca taattggttt aaaatatcct
     9241 tgattttgaa aattcttatc aatcataaaa caaaatagta aataatttcc actactaatt
     9301 acgatctttt tatcataagc tatcatcaca aaacctataa ttacatcatt atcataaact
     9361 gccaatggag ctacaaaatc tccattttta gtgtagacgt atgcttcagc taaactaatt
     9421 gcgttggttg caatgaattg tttttgatat tccttgacat ccaaatttaa aacatcaaaa
     9481 taattttcca ttgtaacatc tcttagttca attgtcatag ttttgctcct tgttagaggt
     9541 tatcattggc gcaaaataat gtttacggca aaccgagata taggtttcat taccaccaat
     9601 ttggatctgt tctccatcgt aaacgggcag tccatcctgt gttcgcaaca ccatggtcgc
     9661 ctttttcttg caatactgac agatggtctt gatttcgtca atcttgtctg ctaaaagcaa
     9721 gagatatttg gaaccttcga acaattcatt gcgaaagtca tttttcaagc caaaagccat
     9781 gacgggtatg tctaactcgt ccacaacacg agctaggtca taaacatggt ggcgtttgag
     9841 aaactgggct tcatcgacca acacacagta aggtttttct ggtaggcctc ggatatagcc
     9901 aaagatatcc gttgtttcct caatcgcaag ggcagggcgt ttcatgccaa ttcgactcga
     9961 cacatagcca acaccgtcac gcgtatccag agctgaagtc ataatcacaa caccttttcc
    10021 ttgctcctca tagttatagg ccaccttgag aatctcgatt gtctttccag agttcatggt
    10081 tccataacga taatacagct gtgccatgtt tcttgcttca cgtccatttc taaatttttg
    10141 ctacattcta gtatatcata attttcttaa gctttaaacg gcaaaatgtg gtaaaatgga
    10201 agaaatcaaa actagtggag gaagctatta tgccatttgt acgcatcgat ttatttgaag
    10261 gacgcacgct cgagcaaaag aaagctcttg ctaaggaagt aacggaagca gttgtccgca
    10321 acactggagc ccctcaatct gctgtccatg tcatcatcaa cgacatgcca gaaggaactt
    10381 acttcccaca aggggaaatg cgtactaaat aagctagctt aagcagaatt gcttaggctt
    10441 tttcaatctc caagtagcat tcattgaaga aatatcctaa atttgttaca atttgaaaag
    10501 aaacttggag aatttccaag aaaatagcta ttaattaaag gaaacattat gattacacgt
    10561 gaatttgata ccatcgctgc tatctctact ccactaggtg aaggggctat tggtattgtc
    10621 cgcctgagcg gaacagacag ttttgctatt gcgcaaaaga tttttaaagg aaaagacttg
    10681 aacaaggttg ccagccacac tctcaactac ggtcacatta ttgatcctct gactggtaaa
    10741 gtcatggacg aggttatggt tggggctatg aagtctccaa agaccttcac tcgtgaggat
    10801 attatcgaga ttaacaccca cggtggtatt gcggtgacca atgagattct ccagctagct
    10861 atccgtgaag gagctcggtt ggcagaacct ggtgaattta ccaaacgtgc ctttttaaac
    10921 ggtcgcgtag atttgacaca ggccgaggcg gtgatggata tcatccgcgc caagacagac
    10981 aaggccatga acatcgcggt caaacaactt gatggttccc tttctgatct tatcaataat
    11041 atccgtcaag aaatccttaa cacacttgcc caagttgagg tcaatatcga ctatcctgag
    11101 tatgacgatg ttgaggaagc cactactgct gttgtccgag agaagacaat ggagtttgag
    11161 caattactaa ccaagctcct taggacagca cgtcgtggta aaatccttcg tgaaggaatt
    11221 tcaacggcta tcattggacg tcccaacgtt gggaaatcaa gccttctcaa caacctcttg
    11281 cgtgaggaca aggctatcgt aaccgatatc gctgggacaa cacgagatgt catcgaagag
    11341 tacgtcaaca tcaatggtgt acctctcaaa ttgattgata cagctggtat tcgtgaaacg
    11401 gatgatatag tggaacaaat cggtgttgag cgttcgaaaa aagccctcaa ggaagccaac
    11461 ttggttctac tagtgctaaa tgccagtgaa ccactgactg cgcaagacag acaacttctt
    11521 gaaattagcc aagataccaa tcgcattatt ctacttaata aaaccgacct gccagaaacg
    11581 attgaaactt cgaaactacc tgaagacgtt atccgtattt cagtccttaa aaaccaaaac
    11641 atcgacaaga ttgaagagcg aatcaacaac ctcttctttg aaaatgctgg cttgattgag
    11701 caagatgcta cttacttgtc aaacgcccgt cacatttccc tgattgaaaa agcagttgaa
    11761 agcctacaag ccgttaatca aggtcttgag ctggggatgc cagttgattt gcttcaagtt
    11821 gacttgactc gtacttggga aatcctcgga gaaatcactg gggatgctgc tccagatgaa
    11881 ctcatcacca aactctttag ccaattctgt ttaggaaaat aagaaaaatc catgatcctt
    11941 cattcggtca tggattttat tgtctttatt agtaatctgg tcttaagacc cctgttacag
    12001 ttgccttagt tgcttcgtag tcgccatcta cgacaacctt gataatgcgt ttggcatctt
    12061 cttctggtgc tggaacaaga ggtagacgag tgggtccagc ttcaaatccc atatagttaa
    12121 gaattgcctt aactggagca ggacttggat aagagaagag agcattaacc ttaggaatga
    12181 atttacgctg aattgctgcg gctttcttca tatcgctttc tgcaatggca gtaaacatct
    12241 cgtgcatttc atccccattt gtatgagagg caacagaaat aaccccatcc gccccaaggt
    12301 tcatggcatg gaaagcatct ccatcctcac ctgtataaat caagaactct tcaggcttgt
    12361 gctcaatcaa gtaagccata ttagccaagc tagtacattc tttgacaccg ataatatttg
    12421 gatggtcagc caagcgaagc atggtttctg gagtcaattc gacaactaca cgccctggaa
    12481 tgttatagat aataattggt aggtcagaag catctgcaat agtcttaaag tgctgataca
    12541 tcccttcttg agaaggtttg ttgtagtaag gaacaatagc aagcccagct gcgaaaccac
    12601 caaattccgc tacttctttg acaaactcaa tagagtcacg cgtatcatta gtacctacac
    12661 ccgcaatcaa aggaacgcgt ccattgacaa ccttttgtac agccgcaaac aactccaact
    12721 cctcatcgtg ggtcaaagtt ggactctcag cagtcgttcc tgcgagaaga attccatccg
    12781 tatgatgggc caataaatgc tcaatcaagg ctggaatagc atcaaagtta ataaaaccat
    12841 cctcatgaaa gggggtaata aaggctgtaa tgattttaca ttcttttaaa tcttgataag
    12901 acatgaaaac acctctctat ttcaaagaag gagttcatct gaactcctaa acgatatgac
    12961 tattttaatt caaatttcaa ttcggctgtt ggacgaacca atccacgttc atgaagagtt
    13021 tcagcaatct gaactgagtt ccaagcagca cctttgagaa ggttatctga aacaacccac
    13081 atgtgaattc ctttttctgc atccaagtct ttacggatac gaccaacaaa ggtatcacgc
    13141 gaaccaactg cattgatagc ttgaggatag atttgatgag ctacatcatc ttcaagaaca
    13201 gcacctggga aggctgcgat agctgctttt acttcttcga ttggagccac ttcttttgtt
    13261 tcgatataaa cagactcaga gtgagctgac aagactggaa tacgcacaca tgttgcagat
    13321 actgcaatgc tatcatcttc cataattttc ttagtttcct tggtcatctt catctcttcg
    13381 tacgtgtaat cattatcagt gaaaacatca atttgtggaa gagcgttaaa ggcgatagga
    13441 tagtgtttct tgtcaccacc tgaaggcaaa atttccgcat gcaaatcaca tggtttcaca
    13501 ccatcattca agacttcacg aagttcacgt tgtgtctcaa gaattgctcc cataccagca
    13561 cctgaaacgg cttgataagt tgaaacaatg atacggtcca agccccattt ttggcgaacc
    13621 ggctcaagag ccaccatcat ttggattgtt gaacaattag ggcaggcaat gattccgttg
    13681 tgggcatcaa gtgcatgagc attgacctct ggaacaacca aaggaacatc tggattttga
    13741 cggaaataag atgtattatc cactactacc acgccagctt ttactgcgta tggtgcatac
    13801 ttagctgatg tagaactacc tgctgaaaag agagcaatat caactccttc aaaagctgtt
    13861 tcagtcgttt cttcaattgt aatatcttga tctttaaatt tcaatgactt acctgctgaa
    13921 cgtgcagaag caaggtaacg gattttatcg attggaagtg ttgattcttc caacattttt
    13981 atcatctgag caccgacagc acctgtcgcg ccgactacag caactgtata tcccataaat
    14041 aacctctttc ggaattttct aaaaatttct ataatagaag tattatacta ctttttccat
    14101 gaattgcaaa gctttttcat cattttttgg aaaataaaaa tcccagtcgc tagactaggg
    14161 actaagaggc atcttcatgt gatgagcagg ttcacacaac tcatcaaggt ccgctcctgc
    14221 gttatgacct ccttatgctc aatagtagtc cgaagactac ctatcgactc atcgcatatt
    14281 ctatattact agagatactt gattttgtca ataagaaatt ttttcgatcg agtataacaa
    14341 aaaactctcg tattcaagag tttccctata catatcatgc taattgccgg gatcgaaccg
    14401 gcgacctcat ccttaccatg gatgcgctct gccaactgag ctaaatcagc ttacttaaga
    14461 agtatactat attcagaaaa acttgtcaag tttccttaga aattttccat aggaaaaagc
    14521 caggtaagaa ctacctagct tatcttcttc tatttactta aatcaattcg acgaccaatt
    14581 ttaccaataa taatcgctcc aataatcaat gaagcaaatt caccgattcc tgttgtaaac
    14641 caagtaagga agaatggtaa ttctgctaca atatttaact ctgcagcgat agtaaacatg
    14701 gaaattgaaa agaggattga aaagaagaaa tgatcttttc gaatcaatcc gttaaagagg
    14761 taatccttgc tatattttgc aaaaagccaa acacctaaac tgaggaatac taaggttgaa
    14821 ccaccaccaa caaagacatc tatcagtccg aagctaaaga aattagcaat catacaaccg
    14881 atggtaactc cgatgatgta cttaggattg taaaaagcca taaagttcat catttctgaa
    14941 atacgaaact gataagcacc atagctgatg gcatttaggg gcggtgtgac agtcaaaacg
    15001 acgtagatag cagcgacgat tgcaatatct gcaacatcac gaatagttaa ttttttcatc
    15061 ttttctcctt tggcggttat ccgcgtgtaa tatgcttggt gaaagaagct aagcaccaag
    15121 ggttgattca ttcaacctta ctagtatagc acaatagcct gctttatgct atacttaaag
    15181 catgaaaaaa cctattcaaa aattttttaa caatgaaatc ttagcctatc tcttttttgg
    15241 ccttgcgaca actctagttt cgattctctc acgactagtc atttatcaac taagtcatca
    15301 ggaactcctt gctacggcct tggcaaatat tatcggtatc ttatttgcct ttatcacaaa
    15361 tgatacgatt gtatttaagc aaaaaaagaa aaatagactt atccgtttag tgaaattttc
    15421 tcttgcccgc ctttctactt ttctgttaga cttgttttta acttttctct ttgtaaccca
    15481 atttcctcac atcatagggc aattcgtaac tgaaaacatt gataagataa atgctattga
    15541 aacaataatt gcacaaatat tcattattat catcaattat atcttgagca aggtctatat
    15601 ttttagaaaa taatgctcca tttatgagcc ttttaattgc cttctataat gatttaggat
    15661 acaatgaaac acgaaacatt gaaaggaaaa cagtatgtta aaaaatctaa aatcgttctt
    15721 gcttcgagga aatgttattg accttgctgt cggtgttgtc attgcctctg cttttggtgc
    15781 tatcgttact tcacttgtaa acgacattat cactcctctt attttaaatc ccgctttgaa
    15841 agctgctaaa gtcgaacgta tcgctcaact ttcttggcat ggagtcggct atggtaactt
    15901 tttaagtgct attatcaatt ttatctttgt gggtaccgcc ctcttcttta ttatcaaggg
    15961 cattgaaaaa gcacagaaac tgactggcat aaagaaagaa aaaactgccg aaaaaaaacc
    16021 aaccgaattg gaagtccttc aagaaataaa agctctcctt gagaaaaaat aatcgattaa
    16081 aaggatctag cgcaaagcta aatccttttt tctttactct ttgggtaact tgcctgcttt
    16141 ttcaagcatt tttttgataa gataaggcat cttaacattc tcccgccctt ttttctcaat
    16201 cagttttttc acaaattctg gcatttgtaa atcttcagat tcagctaaaa tgtttttcgt
    16261 ctcgtctgaa ggaactaact catcaaaggt ctcttcttct gggagaaatt ccttaaactc
    16321 ttgattttca ttgactctaa ctgtattgac caaggcatca atcaaacgag aatctgtatt
    16381 tggcattggt ggacgatggt agtttacccc aaattcctgg cataagtcat aacattctac
    16441 atcattgtca aacaagacct caatatgctc actgatgaaa ctaataggta caaaaatata
    16501 atggtctgga tgttctgtct gttctctgag atactccaaa acatctggtt taatccatgg
    16561 aataccaata tcactctcac tctgccaagt gttggtatat tgttctgaac tcaagcccag
    16621 tttttcagcg actagcttgc tattttcgaa aatttggtct atataaggat caccaaagtc
    16681 caaggcaaaa atgggcacac tatgggctga aaagatgact ttaaagctgt cttgctttac
    16741 ttcttctttt aaaatcttag caatttcatc tgcccaatag tttaagagcg cttcttcctg
    16801 ataccagtcc ttaatgacca aaaattgaat ttgtttgctt tctaaaaatt tttcatagcc
    16861 catgacagag tagaaagaat aatggggctc caaaatcaag caaatacact gttcaattcc
    16921 gtctgcttcc atttgaccga tcacatctgg aataaaggga ctggaaaact tattagcaaa
    16981 gtagacactg tattcattcc ccagcctagc ttctaccaag gcaacctctt gacgggtaat
    17041 tttttgcaga ggcgtgcctc caattcgtac ataattatca taaagagtct gaatctcgtg
    17101 gtcttggggt ctcaccccac gacgaatatt tgtgaaaaaa tcagctacac cttcaaaggt
    17161 aatctcttct ggtgaaccga aggtcatcat taaaattgct tttttcatga ctgcttcctt
    17221 gtgatgtttt tatcaagttt attttatcat taattcatcg ataagtaaac gctagcatag
    17281 ggacattctc aatcttctgt cttttgtttt tctcttcttt ctatgataca atggaaaaaa
    17341 taaattcaaa aggagttttt tatgacttat ccaaatctct tggaccgctt cttaacctac
    17401 gttaaggtca acacgcgctc tgatgaacac tctactacta ctccaagtac acagagtcag
    17461 gttgacttcg caacaaatgt cctaattcct gaaatgaaac gtgttggact gcaaaatgtt
    17521 tactatctac cgaatggttt tgctattgga accttgccag ccaacgatcc gtctttaaca
    17581 cgtaagattg gttttatatc gcacatggat actgctgatt ttaatgctga aggagtcaat
    17641 ccacaggtaa ttgaaaacta cgatggtggt gtgattgaac tagggaattc tggtttcaaa
    17701 ctcgacccag ctgacttcaa gagtcttgaa aaatatccag gacaaacgct catcacaaca
    17761 gatggaacaa gcttgctagg tgctgatgac aaatcaggaa ttgctgaaat tatgacagcc
    17821 attgaatatc taactgctca tcctgaaatt aagcactgtg agattcgtgt tggttttggt
    17881 ccagatgaag aaatcggtgt tggtgccaat aaatttgatg cagaagattt tgatgtggat
    17941 tttgcctaca ctgttgatgg tggtccacta ggtgaacttc agtacgagac tttctcagcc
    18001 gctggtgctg aattgcattt ccaaggccgt aatgtccacc ctggtactgc caaagggcag
    18061 atggtcaatg cccttcagct agcaattgat tttcataatc aacttccaga aaatgaccga
    18121 cctgagttaa ctgaaggtta ccaaggtttt taccatctaa tggatgtgac aggtagtgtt
    18181 gaggaggtgc gtgcaagcta catcattcgt gattttgaaa aagatgcctt tgaagcgcgt
    18241 aaagcatcca tgcaatctat cgctgataag atgaatgaag aacttgggag cgaccgtgtc
    18301 actctcaact tgacagacca gtactacaat atgaaagaag tcattgaaaa agatatgact
    18361 ccaattacca ttgctaaagc cgttatggaa gatctaggta tcacgcctat tatcgaacca
    18421 atccggggtg gaacagacgg ctctaagatt tcctttatgg gaatcccaac tccgaatatc
    18481 tttgcaggtg gcgaaaatat gcacggacgt tttgaatacg ttagccttca gactatggaa
    18541 cgtgcagttg ataccatcat tggcatcgta gcttataaag gctaaaaaga cgaggtagct
    18601 cagctacttc gcctttcttt ttattctact ggtttttctt gatttccagt agttgtagaa
    18661 gattctgttg tttcattttc tgaagttgat tcagcaggtt tagaatctct tgtattgctt
    18721 ggtttgtttt cgtcgctagc agtttcaatg ttagattctg cagttgcgtt tggttggttc
    18781 tcagcactgg tgttatcact atttgcttca gcatttcttg ctggacttgt ttcttcactt
    18841 gcgctagctt ttgactggat ttgatgattc aaaactagaa tagcttttgt cgattcaagt
    18901 aaagctgttt tgtctttact cttagcagaa agttgatcta ataatgcatc caccttatca
    18961 aagtccgcat cagatccatt attactttct aaataagagt gaagcgacat gagaatatcg
    19021 tagagttttt gatagagtac aagtgtctga gaatcttgct cagcattttc cttttcttgt
    19081 tgaagggcgc tagcgatacg agtcaaaaca tcttttacct gactgtttac ttcatccaag
    19141 tctgcatcag ccttgtttgt ggcagctttt agattttcta cttcttctgc caaggattgt
    19201 ctgattcctt cttcatggat ttgttccaag agttgatttg ccttgctcaa aagactttct
    19261 acttcttcct tgctatctgt cgcagattat tggttgctat ctaccatgta ctcctaaaac
    19321 aggagagtta taatccaaga ttacaaggcc ttacagaaat aagaaatcca gataagacaa
    19381 tgttcgtcca agacgctatt cgcttcgcac agcagcacgg attcaatata ctttaatttt
    19441 aaagtttagg tgtcaagacc tctttttagt gtgcccaaaa tttagagaag taatcaatca
    19501 actaactttt atttttttca aactttcagt aaactgacct aaagctaact caatctgtct
    19561 ttgtagatgc ttctgctatc agctagaagt tgatctactt ttgccaagac tgccttctca
    19621 tcaaaagttc caggttgata gttggattgc agggatggaa tcttgttttt caaagccgct
    19681 tcatatccct tagtttgaac cttgatgtag tgattgtggt cgccatgagg aatcacaaaa
    19741 ccttctgaat cttcacttat aattcgattg gcatcaaaaa ccatgaccat cttcttcctc
    19801 atggtggaca tgtagtgacg gattacttaa tacagaacta gaagaacttc ctacctcttc
    19861 cgtgttagag tgtgatgggg gattgttaag agatgactta ggaatatagt gatagtgttc
    19921 cccatgtctt actatataag catcacctat atctctgaca atatcattag ggttaaagac
    19981 atatgtgact gctaattcac ctgccgacaa gtcactctca ggaatgaaat gatagtgacc
    20041 accatgtggt actatagtag gttgaaatag aatatgagca aattgataag gggattttaa
    20101 agtaatttct aacaatgatt tagaaactat gatgtgctat tctaaattca actcactata
    20161 tataaccatc atcggtagta taacgtccct gtaattttgc tacagatact tctgcactag
    20221 ctcctttatc gtctttacca tgttcttgtt tttggcgatt gatttcatct tttgttcgta
    20281 cattttctgc atgagcttga tctttaaggt aaacataata ctttccatct accttaataa
    20341 tatatcctcc cttaacctaa ctgacgatat cttgatcttt cggctgatag ttgggggctt
    20401 tcattagtag ctcttcacta aagagcgcat caaaaggaac tttaccatta tagtagtgat
    20461 aatgatcgcc atgagaagtt acataacctt gatctgtaat cttaataaca atttgttttg
    20521 cttgaattcc ttctttttga ctaacctagt ctggagtcaa attttcagtc ttcttagtgt
    20581 ctttattact gtttacatat gaaacacgat ttttatctgt attggcctgt tagctatgtt
    20641 ggttcagagc ataaacacac agacttaagg aaaggataac aacagatcca gctgctatat
    20701 atttcttttt aaatttcata attacctcat ttctat
//
LOCUS       DAVKJU010000035        20096 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVKJU010000035 DAVKJU010000000
VERSION     DAVKJU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.035"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..726)
                     /gene="srtA"
                     /locus_tag="VW191_001232"
     CDS             complement(<1..726)
                     /gene="srtA"
                     /locus_tag="VW191_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEU9624001.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQP"
     gene            complement(726..3194)
                     /gene="gyrA"
                     /locus_tag="VW191_001233"
     CDS             complement(726..3194)
                     /gene="gyrA"
                     /locus_tag="VW191_001233"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU9624002.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            3388..4374
                     /locus_tag="VW191_001234"
     CDS             3388..4374
                     /locus_tag="VW191_001234"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEU9624003.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            complement(4637..5411)
                     /locus_tag="VW191_001235"
                     /pseudo
     CDS             complement(4637..5411)
                     /locus_tag="VW191_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
     gene            complement(5458..5712)
                     /locus_tag="VW191_001236"
     CDS             complement(5458..5712)
                     /locus_tag="VW191_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEU9624004.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            complement(5714..5956)
                     /locus_tag="VW191_001237"
     CDS             complement(5714..5956)
                     /locus_tag="VW191_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEU9624005.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            complement(6047..6856)
                     /locus_tag="VW191_001238"
     CDS             complement(6047..6856)
                     /locus_tag="VW191_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU9624006.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            complement(6858..8207)
                     /gene="vicK"
                     /locus_tag="VW191_001239"
     CDS             complement(6858..8207)
                     /gene="vicK"
                     /locus_tag="VW191_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEU9624007.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            complement(8200..8904)
                     /locus_tag="VW191_001240"
     CDS             complement(8200..8904)
                     /locus_tag="VW191_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9624008.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            complement(8959..10134)
                     /gene="mutY"
                     /locus_tag="VW191_001241"
     CDS             complement(8959..10134)
                     /gene="mutY"
                     /locus_tag="VW191_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEU9624009.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            complement(10463..12133)
                     /locus_tag="VW191_001242"
     CDS             complement(10463..12133)
                     /locus_tag="VW191_001242"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU9624010.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIAQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            12363..13052
                     /gene="coaB"
                     /locus_tag="VW191_001243"
     CDS             12363..13052
                     /gene="coaB"
                     /locus_tag="VW191_001243"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEU9624011.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLFAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            13064..13615
                     /gene="coaC"
                     /locus_tag="VW191_001244"
     CDS             13064..13615
                     /gene="coaC"
                     /locus_tag="VW191_001244"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEU9624012.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            13599..14162
                     /locus_tag="VW191_001245"
     CDS             13599..14162
                     /locus_tag="VW191_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9624013.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            14444..14971
                     /locus_tag="VW191_001246"
     CDS             14444..14971
                     /locus_tag="VW191_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9624014.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAIFVILASSFGFLMAGYPMVIVFRAFSHIFFGTLGALYLQKFPDTL
                     DKPKASWIFNFVLALVHALAEVLACVVFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            14983..15498
                     /locus_tag="VW191_001247"
     CDS             14983..15498
                     /locus_tag="VW191_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEU9624015.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYVYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGRVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(15495..15962)
                     /locus_tag="VW191_001248"
     CDS             complement(15495..15962)
                     /locus_tag="VW191_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9624016.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(16035..16523)
                     /locus_tag="VW191_001249"
     CDS             complement(16035..16523)
                     /locus_tag="VW191_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624017.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKTALTALNRFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKMLELGKPIKEKRMVFY
                     SK"
     gene            complement(16489..17052)
                     /locus_tag="VW191_001250"
     CDS             complement(16489..17052)
                     /locus_tag="VW191_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624018.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQCDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(17062..19050)
                     /gene="uvrB"
                     /locus_tag="VW191_001251"
     CDS             complement(17062..19050)
                     /gene="uvrB"
                     /locus_tag="VW191_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEU9624019.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            complement(19127..20080)
                     /locus_tag="VW191_001252"
     CDS             complement(19127..20080)
                     /locus_tag="VW191_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9624020.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         5979 a         4501 c         3510 g         6106 t
ORIGIN      
        1 tggttgattg aaagctgtta ggatttcatt agatgtttgt gaataatctt ttgtttcttt
       61 caaatcacct ttgacaataa tacgttctgt agcagcaagg tcttcacagg ttactaatgt
      121 gatttcattg accccatctc tatcatcaac ttcatcaaca cgatccggtg tcacacgttt
      181 gacttcacgt atttcataag tataaacttt atttttatcg gttagataaa tcttcatgcc
      241 atttttagca ttatctaaag gagaaaataa cattttatta gcattatcaa caccaaagat
      301 atggtgacta gctagactat aatttccttc tcccattact tgctcgcgtt tcattgtacc
      361 agctccgtag aagagattaa cattatcaag tcctttaaaa atcggcaaat tcatttccaa
      421 ttcaggaatt gcaattcccc caataactgg taatttttga gcatcccatt gagaagttag
      481 aacagcttcc gaagagatag ctttgacaga atcaaagtca aaattgcctt ctgtatcctg
      541 attttcttct aatttttctt ttgatacctg gctaacttga tacttattgg tattccagac
      601 tatgaaaata tttcgaattt gagtattaaa aatcaaagcc agtgacagta atatcagaaa
      661 tcctgctagg atatttgtca gcagattttt tcgcttgttt ttctttttat tatttttttg
      721 agacattatg cttcaccttc tgtttcgttt tctgtcccaa cttcttcttt ttctgccacc
      781 gcaaccgttg tgaaagtcac tatctgagca tcttgatcca ggcgcattac tttaactccc
      841 atagttgcac gtcctgtttg tgaaatattg gcaagattgg ttcgaatcat gacacctgta
      901 tcagtgataa tcatcaaatc ctcatcccct tgaacagtca taagaccggc cagcaagcca
      961 tttttttcgg taattttagc tgtctgcatt cccttaccac cacgaccttt tgttgggtat
     1021 tcagtagcga ctgtacgctt accatatcct ttttctgtga taataagaac ctcatcttga
     1081 tcagtaatca agctggcacc aacaactgtg tctccttcac gaaggttaac acctttcaca
     1141 ccagtggcga tacgactcat accacgaacg gctgattgat taaagcgaac tgcataacca
     1201 aacttggtac caatgataat atccatatct ccttctgcca acaagacatt gattaactca
     1261 tcttcatcct ttaaattcag cgctttgaga ccattttgac gaatattggc aaactcctta
     1321 acactggttc tcttcacaat accgtgacgg gttgtaaaga agagataagc atcatcactg
     1381 cgatcagact caacattgat aaccgtctga atactttcgt cttcatccaa tttcaagaga
     1441 ttgactactg gtagcccttt ggcagtccga ccatactcag gaatttcata acctttaaga
     1501 cgatagacac gtcccttgtt tgtgaagaag agcagatgat catgggtgct agttgacact
     1561 aactcacgaa caaagtcatc atctttcact cccgttcctt ggacaccacg acccccacgt
     1621 ttttgagcag tgaactcgtc ctgatccaaa cgcttaatgt agcctctgtt agaaagggta
     1681 atcaagacat ccgattcttc aatcaagtcc tcatcctcga gactcaagac ctgtccaatc
     1741 atcaactctg tacggcgctt atcagaaaat ttacgtttaa cttcatccaa ttcgtctttg
     1801 ataatttgag aaacacgttc aggcttagca agaatatctg ctaaatccgc aatcagagcc
     1861 aagaggtcat catactcaga ttgaatctta tcgcgttcca aacctgtcaa acgacgaaga
     1921 cgcatatcaa ggatagcttg actttgacgt tcagaaagct taaacttgct catcaactca
     1981 gcttgagctt ccgcatccgt ttcactagca cggatgatac gaatcacttc gtcgatatgg
     2041 tctagcgcaa tcaagagacc ttctaagata tgagcgcgcg cttccgcttt ttccttatca
     2101 aaacgtgtac gacgaacaac cacttctttt tggtgctcga tataagcatc caaaatctga
     2161 cgaagagaca aaattttcgg tataccattt tggatagcga gcatattgaa accaaaattg
     2221 gtttgcattt gggtcatttt gaagaggtta ttgagaataa cattggctga ggcgtcgcgc
     2281 ttgacttcaa taacaaatcg aacaccttca cggtttgact catcacgtac tgctgtgata
     2341 ccctcaatgc gtttttcctg aaccaagcga acaatatgct catgcacctt ggttttattg
     2401 accatgtaag gaaattctgt tacaacgata cgctcacgac cagtcttagt cgtttcaatc
     2461 tctgtacgag aacgtaggac aatcgaacct ttacctgttt cataagcctt atggatacct
     2521 gatttcccca tgacaagagc accagttgga aaatctggtc caggcaagac ttccatcaag
     2581 tccttggtag tcacttcagg attatccatg accaacttca ctgcatcaat ggtttcaccc
     2641 agattatgag gtggaatatt ggttgccatc ccaaccgcga taccagttgc tccattaacc
     2701 aaaaggtttg gaaaacgcgc tggcaagacc aagggttccc gttcattggc atcatagtta
     2761 tcaacgaaat caactgtatt tttgttgata tcacgaagca tttccagagc aatcttgctc
     2821 atacgtgcct cggtataacg ttgagcggca gcactatctc catccatgga accaaaattc
     2881 ccatgaccat ctacaagcat gtaacggtag ctccaccatt gagccatacg gaccatggct
     2941 tcataaatag aggaatcccc gtgtgggtga tatttaccca tgacatcccc tgtaatacga
     3001 gcagattttt tatggggttt gtctggggtc acacccaatt cattcattcc gtagagaatg
     3061 cgacggtgaa caggttttaa gccatctcga acatcaggaa gagctcgcgc tacgataaca
     3121 ctcatggcgt agtcgataaa acttgccttc atctcctttg tcagattgac attcactaaa
     3181 tttttatcct gcattaataa atgcctcatt tcacaattag taagtaacaa cattatacca
     3241 taaattccca tctatttcag cctctaaacc actaaaacgt ttacatcgag aactataagg
     3301 catattcgtg acaaagtttt ttaaaagtga tagaatgaag ttgtctaggg aaaatcccct
     3361 aataaaatta aggagatgtt taaaacaatg acttcaacta aacaacacaa aaaagttatc
     3421 cttgtcggtg atggtgctgt aggttcatct tacgcttttg cacttgttaa ccaaggaatt
     3481 gcacaagagc ttggaattat cgaaattcca caattgcatg aaaaagctgt tggtgatgcg
     3541 cttgacctta gtcacgccct tgccttcact tcacctaaaa aaatctatgc agctcaatac
     3601 tctgactgtg cagacgctga ccttgttgtg atcactgcag gtgcacctca aaaaccaggt
     3661 gaaactcgtc ttgaccttgt aggtaaaaac cttgctatca acaaatcaat cgtaactcaa
     3721 gttgttgaat ctggtttcaa aggtatcttc cttgttgctg ctaacccagt tgacgttttg
     3781 acttactcaa cttggaaatt ctctggtttc cctaaagaac gcgttatcgg ttcaggtact
     3841 tcacttgact cagctcgttt ccgtcaagca cttgctgaaa aattggatgt ggatgctcgt
     3901 tcagtgcacg cctacatcat gggtgaacac ggtgactctg agttcgctgt ttggtcacac
     3961 gctaacatcg ctggtgtaaa ccttgaagaa ttccttaaag acactcaaaa tgttcaagaa
     4021 gctgaattga ttgaattgtt cgaaggtgtt cgtgatgcag cctacacaat catcaacaaa
     4081 aaaggtgcaa catactacgg tatcgcagta gcccttgctc gtatcactaa agcaatcctt
     4141 gacgatgaaa acgcagtact tccactttca gtattccaag aaggtcaata cggagttgag
     4201 aatgtcttta tcggtcaacc agctgttgtt ggtgcacatg gtatcgttcg tccagtaaac
     4261 attccattga acgatgcaga aactcaaaaa atgcaagcat ctgctaaaga attgcaagct
     4321 attattgacg aagcatggaa aaatccagaa ttccaagaag cttctaaaaa ctaatttaac
     4381 tatagacaac tccttgagtc atcagggagt tgttttttcc tgtattatat gaaatagtac
     4441 cccattactc taaaacgttg ttataaaatg ctttaaattc ctaatcgatt tgttcatatc
     4501 ttatttcaat ccattataca tcttcccaca ataaaacgca tagtatcaag tttttatacc
     4561 tgataatatg cgttctattg tgtcaaaact tacttgattt ctcctcagtt caaacttttt
     4621 ctcatcttta tttttattat gttttttcaa tcaaatcttg tatcatttta tgtagttctt
     4681 ctatcttttt agattgtttc tctagtaata ttctcggact agataattca tcttggagtt
     4741 tatcaagttt tatctgttca tctggattat cctttacaaa aaaagttcgt caaagcactt
     4801 gttaacatcc ctattgttcc aataccgaca agcatcagta aaactgctag ccatttcccc
     4861 aaaagggaaa ctggaacaat atcaccataa ccaacagtag ttactgtcac aagagcccaa
     4921 caaagactat ctgagaagga tttttcttca actacagata aaattgaact tcctactaga
     4981 ataataaaga tattaacata taagatataa atcaggccat ttgtccgtaa gaatctacta
     5041 atttttcttt ctaatttacc tgtaagtcca attattctaa gtaaacgagt cagtttcagt
     5101 aattttgtta gtttaactaa acgaaagata cgccctaatc gaaaaactgt gaaaatagca
     5161 tttaaaggga gaatagccaa taaatcaaaa acattttcaa gaataaagcg ccattttctt
     5221 ttagtaataa aaaagcgcca actataatca attacaaaaa caccccagat tactaaatca
     5281 ataatactat aaggcggatt gtccaaatca atcatctcag caaatccaag taataccaga
     5341 ataacagaga ttaaagcaag taaaataatt gttgtatcat aataatctgc aaaaaaccat
     5401 tttttcttca aatttttact ctccttactt tctatattgt tattactcag ctttaattca
     5461 ataaatatct ctccgatgac caacttctag agtagcaaca actaattcat catctacaat
     5521 ttgtacgata actcgataat taccaattct atagcgccat tgaccaacgc gattaccaac
     5581 caaagccttt ccgtgtcgtc ttgggtcttc caaaacattg atttgtaaat agtttgtaat
     5641 tagcttctgc gtataacggt ccaatttttt caattgcttg atgaaacgtc ttgttggaac
     5701 taatttatac aaattattca tccttcaagc ctaaatcatg catcatttct tcccaagtaa
     5761 tgggttcaac tcctttttcc aagtcttcta aatactcttg ataggctaaa tctgccacac
     5821 gagcatcgta ttcatcttct agggcttcaa gagttttggt gcgaataagt tccgaaaggg
     5881 aaactccttc aaacttagcc attgctttca taaatgtttt atcagcttca gaaactttta
     5941 atgtaatagt agtcatcttt tgtgctccct tttttaatgg taacaccatt gtattacttt
     6001 ttaggtgttc agtcaatata aaaagaacac cttctcagcg ttctttctat atctctgtca
     6061 atggtgttgc ggtatctggt gaggtatcat aaaccttaaa gtctactccg actcccagat
     6121 cagcttgagc cagctgattg accatggtca tatgagccag ttccttgata ttgttttcct
     6181 tagataaatg cccaaggtaa atcttcttag tacgatttcc tagcgtccga atcatagctt
     6241 cagcaccgtc ctcgttagaa aggtgaccaa ggtcagatag gattcgttgt ttgagtcgcc
     6301 aagcgtaaga acctgatcgc aaaatctcta catcatggtt ggcctcgata agatagccat
     6361 ccgcattttc gacaatgccc gccatacggt cactgacata acctgtatct gtcaaaagga
     6421 caaaactctt atcatccttc ataaagcgat agaactgcgg tgcgactgca tcatggctta
     6481 caccaaaact ctcgatgtcg atatctccaa aggttttggt tttacccatt tcaaaaatat
     6541 gcttttgcga agaatccacc ttgccaagat atttactatt ttccatagct tgccaggtct
     6601 tttcattggc ataaagatcc ataccatact tgcgagccaa aacgcctact ccatggatat
     6661 gatctgaatg ctcatgggta atcaagatgg catccaggtc ttctggctta cggttaattt
     6721 cagctagcag actggtaatt ttcttgccag acaagcctgc atctactaaa agcttctttt
     6781 ttgaggtttc cagataaaaa gaatttccac tggaacccga cgctaaaata ctgtatttaa
     6841 agcctatttc actcattcta gtcttctact tcatcctccc atacttcttc tttcactgca
     6901 tccttatcat aagggagcac aatggtaaag gttgaaccct tgccgtattc actcttggcc
     6961 caaataaagc ccttatgttg tttgataatt tctttagcga tagacagtcc tagacctgta
     7021 ccaccttgtg cacgacttct agcacgatcc acacgataga aacggtcaaa gatacgtggt
     7081 aaatcctgct taggaatccc caaaccgtgg tcagaaatgg ataaaatcat ctggtcttca
     7141 gttgtcttca ttctgacagt gattttaccc ccatctggcg aatacttaat agcattattt
     7201 aaaatattgt cgacaacctg cgtcatctta tctgtatcaa tttccatcca gatagaattg
     7261 atgggataat ctctcaccaa ctcatatttt ttctcctttt cctgtccttt catcttgtca
     7321 aaacgattga ggataaaggt aataaaagca gtgaagttaa tcagttccac atctaggtga
     7381 ctggtagcat tatcaatacg tgaaagatgg aggagatccg tcaccatgcg catcatacgg
     7441 ttggtctcat caagagaaac cttgataaag tctggtgcta cagtttcaca caaagccccc
     7501 tcatccaagg cttcaagata ggattttacg ctagtcagag gagtccgtaa ctcatggcta
     7561 acattggaaa caaagagtct tcgttcgcgt tcttccttct cctgctccgt cgtatcatgc
     7621 aaaacagcca ccaaacctga aataaagcca gactctcgac gtatcaaggc aaagcgaact
     7681 cgaaggttca aatattcgcc attgatatct tgggaatcta gcaacaattc tggactttgg
     7741 gtaatcaaat cacgcaattc atagttttct tctatcttga gcaattccaa aatgcttcta
     7801 ttcagaacat cttccttaac caaccccagt tgcttcttgg ctgtatcgtt aatcatgata
     7861 atctgacccc gacggttggt cgcaagaacc ccatctgtca tataaaacag aatactattt
     7921 agcctcttac tctcttgttc tagattttcc tgagtgagac gaataacctc cgacaagtca
     7981 ttcaaattat tggtaatatt ggtgatttca gacccacctt gcatatcaag aaccttggaa
     8041 taatctcctg caatcaaatc tttaaccttt tgattgattt gcttcaactg aatattatca
     8101 cgtctatttt ccagtaataa gagggtcaca acaaggatga aacctaacaa aatcaggata
     8161 aagataaaat ctctggtaaa aatggtttgt ttcagtaaat caagcattat ttctcatgta
     8221 ataccctaca ccacggcgcg tcaagatata ctctggtcgg ctgggcgtat cttcaatctt
     8281 ctcacgcaga cgtcgtacag tcacatcaac tgtacggaca tcaccaaaat agtcataacc
     8341 ccagacagtc tcaagcaagt gttcgcgcgt gatgacttga cctgtatgcg atgctaaatg
     8401 atacaaaagc tcaaattcac gatgggttaa gtctagttct tcgccatatt ttttagccac
     8461 gtaggcgtct ggaacaattt ctaaatcccc aatttggata ggttgaggtt tactatctgc
     8521 ttcctgacca tctactggca taggttgaga acgacgcaga agagctttaa cacgcgcctg
     8581 caactcacga ttggagaagg gttttgttac atagtcatct gccccaagtt ccaaaccgat
     8641 aaccttatca aattcactat ctttggctga aagcataaga atgggcacac tgcttgtctt
     8701 acgaatggtc ttagcaactt ctaaaccatc aatttctgga agcatcaaat ccagaataat
     8761 aatatctggt tgctctgctt caaattgctc tagcgcttca cgaccattaa aagcagttac
     8821 aacttcgtaa ccttccttgg tcatattaaa cttgataata tccgagattg gtttctcatc
     8881 atctacaatt agtatttttt tcatatgttc acctttttct ctactattat accaaaaaat
     8941 agtcagaaga cacaatagct agtcttggct actgtctaag ttggcttgtg cataagcctg
     9001 ccagattttt tgttggggtt tggcaagtgg gtaattcttg aattcttctg gtgaaagcca
     9061 acgaacttcc ctatctgaaa aatcatggaa atcactcacc tgacctgcta caatctgtac
     9121 atgccatttt cgatgactaa aaacatgctg gactgtatca aaacaaacat caagccaatc
     9181 aacatctagg tcatagtcct gctggaaact ctcttctgga ctggggccag agttcacact
     9241 ttcttccgca acctgatgaa agaggtcaaa ctgctcttct tgcgaaaagt tatcaacttc
     9301 tataaagggg aaatgccaaa aacctgccaa gagcttttcg ctttcatttt tttcaagtaa
     9361 aaattgtcct tgagaatttt tcacaactaa ggctttaaga taaataggaa ccggcttttt
     9421 cttaggagat ttaattggat aacggtccat ggttccattc tgatatgccg cactaaagtc
     9481 cttgactggg ctttcttcag gtctgggatt tacaggagac tcaatatcag accctaagtc
     9541 catcaaggct tgattaaaat cacccggacg atctggatta atcaagatct ccatcattgc
     9601 ctgaaaaatt tttcgattac ttggaatccc aatatcgtgg ttgacttcaa acagacgcgc
     9661 caagacccgc atgacattac catctacagc tggctcaggc aagttaaaag caatactgga
     9721 aatggctcct gctgtgtaag gtccaatccc tttcaagctg gaaattcctt cataggtatt
     9781 tggaaattgg ccaccaaagt cagtcataat ctgctgggct gcagcctgca tattgcgaac
     9841 tcgagaataa taacccaagc cctcccaagc tttcagtaaa ctctcctcag gcgcagttgc
     9901 cagactttcg acagttggaa accagtccaa aaatctttcg tagtaaggga taactgtatc
     9961 caccctggtc tgctgaagca tgatttcaga tacccagatg tgataaggat ttttacttct
    10021 cctccaaggc aaatctcttt tgttttcatc ataccaagcg agaagtttct cacggaaaga
    10081 aatgactttc tcctccggcc acatgacgat accgtattct ttcaaatcta acatatctct
    10141 agtataacac agaaggtttc acctgtcttt gtatctgatt tataatattt tcaatagata
    10201 gtatataact tttctatcta cttatactca atgaaaatca aagagcaaac taggaagcta
    10261 gccgcaggtt gctcaaaaca ctgttttgag attgtggata gaactgacag agtcagtatc
    10321 atattaccta cggcaaggtg aagctgacgt agtttgaaaa gattttcgaa gagtataaat
    10381 cttattgatg aactgcttgc agtctgagaa aaaatgagct tggatattat ttccaaactc
    10441 acttaaagtc aatttcaatc cactagaaca agcctagcac agttccatcg ctttcaacat
    10501 ccatgttgag agctgctgga cgttttggaa gacctggcat ggtcataaca tcaccagtta
    10561 aggcaacgat gaagcctgca cctaattttg gtaccaattc acgaatggta atttcaaagt
    10621 tttctggtgc tccaagcgca tttggattgt ctgagaaact gtattgagtt ttagccatac
    10681 aaattggcaa tttgtcccaa ccgttttgaa cgatttgagc aatttgtgtt tgagctttct
    10741 tctcaaagtt cactttgcta ccacgataga tttcagtgac aattttttca atcttttctt
    10801 ggacagaaag gtcattatcg tacaaacgtt tatagttagc tggattttca gcaattgtct
    10861 taacaactgt ttcggcaagt gctactccac cttctgctcc atcagcccag acactagcca
    10921 attcaactgg tacatcgatt gaggcacaga gttcttttaa gactgcaatt tcagcttctg
    10981 tatcagatac aaattcgtta atagctacaa ctgctggaat accgaactta cggatatttt
    11041 caacgtggcg tttcaagtta gcaaaacctg cacgaactgc ctctacattt tcttcagtca
    11101 gagcgtcttt agccacacca ccattcatct taagggcacg aagggttgcg acaataacaa
    11161 ctgcatctgg agatgttggc aagtttggtg tcttgatatc aaggaatttc tcagcaccaa
    11221 gatccgcacc aaaaccagct tcagtaacag tgtaatcagc caagtgaagg gctgttgtcg
    11281 tcgccaaaac agagttacag ccatgagcga tattggcaaa tggaccaccg tgtacaaagg
    11341 caggtgtacc gtaaattgtc tgaaccaagt ttggcttaat agcatccttc aaaatcaaag
    11401 ccaaggcacc ctcaacctgc aaatcaccta cagaaacagg cgtacggtca tagcgataac
    11461 caataacgat attcgccaaa cgacgtttca agtcctcgat gtccgttgcc aagcaaagaa
    11521 ttgccatgat ttctgaagca actgtaatat caaaaccatc ctcacgtgga ataccgttta
    11581 gaggaccacc aagaccaaca gtcacatggc ggagcgcacg gtcgttcaag tccacaacgc
    11641 gtttccagag gatacgacgt tgagcaattc ccagctcatt cccttggtgc aagtggttgt
    11701 caatcaaggc agaaagggca ttgttggcag ttgtaatagc atgcatatct ccagtaaagt
    11761 ggaggttgat gtcttccatt ggcagaactt gtgcataccc accaccagca gcaccaccct
    11821 tgatccccat gactggacca agagacggtt cgcggatagc aatcatggtt ttcttgccaa
    11881 tcttgttcaa ggcatccgca agaccaatgg taagcgtcga ctttccttca cctgcaggtg
    11941 ttgggttgat ggcagtaacc aagatcaatt taccgactgg attgctctca actgcacgaa
    12001 ttttatcaaa gctgagttta gccttgtact ttccgtacaa ctccaaatcg tcgtaagaaa
    12061 taccaagttt ctctacaaca tcaacaattg gcttcaactc aatactctgt gcgatttcaa
    12121 tatctgtttt cattcaaaat tcctctaacc tcttatatga taattcatta tatcacaaaa
    12181 caagattttt aacatcctaa aactctctaa acgttcgtaa atatctctgt ttttaagact
    12241 tttagagtcc tttcttaaat tttatatggc tttatagttt gaaactataa taaattttcg
    12301 tttttaccaa aaatttatca ctttcatttt acttaccgct tatttttgtg tacaatagtg
    12361 ctatgaaaat tttagttaca tcgggcggta ccagtgaagc tatcgatagc gtccgctcta
    12421 tcactaacca ttctacaggt cacttgggga aaattatcac agagactttg ctttttgcag
    12481 ggcatgaagt ttgtttaatt acgacaaaac gagctctgaa gccagagcct catcctaacc
    12541 taagtattcg agaaattacc aataccaagg accttctaat agaaatgcaa gaacgtgttc
    12601 aggattatca ggtcttgatc cactcaatgg ctgtttctga ctacactcct gtttatatga
    12661 cagggcttga ggaagttcag gctagctcca atctaaaaga atttttaagc aagcagaatc
    12721 atcaggccaa gatttcttca actgatgagg ttcaggtttt gttccttaaa aagacaccca
    12781 aaatcatatc cctagtcaag gaatggaatc ctactattca tctgattggt ttcaaactgc
    12841 tggttgatgt taccgaagat catctggttg acattgcacg aaaaagtctt atcaagaatc
    12901 aagcagattt aatcatcgcg aatgacctga ctcaaatttc agcaggtcag caccgagcta
    12961 tatttgttga gaaaaatcag cttcaaacag tccagactaa agaagaaatt gcagaactcc
    13021 tccttgaaaa aattcaagcc tatcattctt agaaaggaaa actatggcaa acattctctt
    13081 ggctgtaacg ggttcaatcg cctcttataa gtcggcagat ttagtcagtt ctctaaaaaa
    13141 acaaggccat caagtcactg tcttaatgac tcaggctgct acagagttta tccaaccttt
    13201 gacactacag gtactctcac agaatcctgt ccacttggat gtcatgaaag aaccctatcc
    13261 tgatcaggtc aatcatatcg aacttggaaa aaaagcagat ttatttatcg tggtacctgc
    13321 aactgctaac actattgcaa aactagctca cggatttgcg gacaacatgg taaccagtac
    13381 agctctagcc ctaccaagtc atattcccaa actaatagct cctgctatga atacaaaaat
    13441 gtatgaccat ccagtaactc agaataatct gaaaacatta gaaacctacg gctatcagct
    13501 gattgctcct aaggaatccc tactagcttg tggagaccac ggacgaggag ctttagctga
    13561 cctcacaatt attttagaaa gaataaagga aactatcgat gaaaaaacgc tctaatattg
    13621 cacccattgc tatctttttt gctaccatgc tcgtgataca ctttctgagc tcacttatct
    13681 ttaacctttt tccatttcca atcaaaccga ccattgttca tattcctgtc attattgcca
    13741 gcattattta tggtccacga gttggggtta cacttggatt tttgatggga ttacttagct
    13801 tgacggttaa cacgattacg attctaccga caagctacct cttctctccc ttcgtaccaa
    13861 acggaaacat ctactcagct atcattgcca ttgtcccacg tattttgatt ggtttaactc
    13921 cttacttagt ctataaactg atgaaaaaca agactggtct gattttagct ggggctcttg
    13981 gttccttgac aaatactatc tttgtccttg gaggaatctt cttcctattt ggaaatgttt
    14041 ataatggaaa tatccaactt cttctggcaa ccgttatctc aacaaattca attgctgaat
    14101 tggtcatttc tgcaattcta accctagcca ttgttccacg actacaaacc ttgaaaaaat
    14161 aaaaacaatc tccgctttca aacctgaagg tggagatttt gctctataca gtttcttttt
    14221 aatgaaatct ttactatagt aaaacgaaat aagaacagga taaatcgatc aggacagcca
    14281 aatcgatttc taacaatgtt ttagaagtag aggtggacta ttctagtttc aatctactat
    14341 aacttttaac taaaaaatga tctataaacc caagtaaatt cttgctttat tgtctggttt
    14401 attgtaccat actagtgtat atacagttaa aaaggagatt cttatgaaca cacggaaaaa
    14461 gacacaattt atgacaatga cagccctctt aacggctatt gcgattttga ttccaattgt
    14521 tatgcccttt aagattgtca ttcctcctgc ttcttacact ttggggagcc acatcgctat
    14581 ttttatcgcc atgttcttgt cacccttgat ggcaattttt gtcatcctag cctctagttt
    14641 tggatttttg atggctggct atcccatggt tatagtattt cgagcttttt cccatatctt
    14701 ttttgggact ttgggggctc tttacctaca aaaattcccc gataccttag ataaaccaaa
    14761 agcctcctgg attttcaact ttgtgttagc gcttgtccat gcccttgctg aagtattggc
    14821 ctgtgtcgtt ttttacgcaa cttctggtac caatgtagaa aatatgtttt atgttctatt
    14881 tgtgctagtt ggatttggca caattatcca tagtatggta gactatacat tagcactagc
    14941 tgtctataaa gtgcttcgaa aacgccgtta aaaggaaaag ctatgacaaa agatcgcaaa
    15001 caagctctgc tccaactctt gaaagaagct cctaaagccc ttaatggcca aagtttggcg
    15061 gaacattttc atgtcacacg tcaggtcatt gtgcaggaca ttgcaatttt gagagccgat
    15121 ggcgctccta tcctatccac caatcgtggc tacgtctata agcaaattga taccaatcct
    15181 tatgtccata aacttttcaa ggtgaaacat gaagttgaag aaatcggtca agaactcctt
    15241 gctatcgttg ataatggtgg acgtgttcaa aataccttga ttgaccatcc cgtttatgga
    15301 gaaatcgaaa ccttgctgaa actgtcttgt cgccgagacg tgcaacattt tctagaacaa
    15361 gtcgagcatt ctgattttaa acctttatct gaattgacag atggtgtcca ttaccaccta
    15421 gtcgaagccg aaacacaaca agacctccac tatatcgagg aggccttgga tcagctaggt
    15481 tatttagtaa aggattagaa aattttcttt tcccaatcgt cttctgtacg gcgagggtag
    15541 aaaaattcag atttgtccga tgcttccatc atctccatca agggtagcat atcataggcc
    15601 agatccaaat ttggaatctg gtctttttgc acccaagaaa cttctccttc atctgaagag
    15661 cgaagggtac cagagaactc agttgcctta taacaaacga caatatagcg cccacctgta
    15721 tctagtggcc aatttttaat gccgacaagt tgaggatttt ggatagtcaa ccctgtttct
    15781 tcatagattt cacgaatgac agactctgcg aaagattcgc cattttctac atggcctcct
    15841 ggaaaggcat aaccggacca gcgattagtt tcaggagcgc gatactgcat caccacgcgc
    15901 tgagcttcga gatcttcaat cagacaaata tttgttaaaa tcgttaattg tgctcgagac
    15961 ataaatttac tcactttcta atttattaaa tttttagact tggtaccagt accattagct
    16021 tctacttttt aaaattattt agaatagaat accatccgtt tttcctttat gggctttcca
    16081 agttcaagca ttttttcttg accatcaaaa gtaaaaccca ttttttgata gaaagcaatg
    16141 gctcgcttgt tatctttcaa tacccataag aaaatttcag aaaaacgatt cagagcagtc
    16201 aaagctgttt tcattaactt ttgtgctatg ccttttccat aatagtcttt taaaacatat
    16261 aaagcaataa tttcaccagc ttgaatagtc tcatcacgaa agttaccata actgataaaa
    16321 ccaactacct tcagatcacc tatcgcaatc aatgtatttt ctggatactt ttgactaaag
    16381 aatcgacatc tttctaatgt cattgtctcc tgaaattctg caggcaaaag gtcatcataa
    16441 gcctctctcc acgtttgcca gtgaacgagg gatttacctt ctatctcttc aggagtttcc
    16501 attgatttga taataaccgt catttatttt ctcccagtct tctctcaaaa taccatattt
    16561 aatactatca aaatatttac cttgataata acgaactttt ggaatatgag cttctttttt
    16621 cattcttaat ttttcagcaa gtttcatcat accaagattt cctgaccaag ttgtcaaacc
    16681 cagatgctcc aactccaagt aatcctgaaa cgtcctatct atccactgca acatagcaac
    16741 tttcccaata ccagtgttcc agaatttttt atcataaata ccaattccca attccatcca
    16801 tcttgtttgt ttacataccc aataacgcga aacagtccca actagtttat catcaacaaa
    16861 aataccaagg cgatttgagt tgcttaaaat ggattctgat ttttgtagtt cgaattcttt
    16921 aaaatttgaa aaatactgat aatcatcata atagggagca tcacactgtt tccaaactgg
    16981 attaagttgt gaataagcca tttcccataa agacatcaaa tcttctctct ttaactttct
    17041 taaataaatc atactattcc cctaatccaa ggccttgact tccaacatca tatcacggat
    17101 ctgagccgcc agttcaaagt caagcacttc gacggcttct tgcatttgtt tttctagttt
    17161 cttgacgagt tctttgcgct cttgtttgtt gaggctattg atgtcgactt ccttgtcctc
    17221 ttccttcgca actgccttgg tcacggcaat caggtcacgg atttctttct tgattgtctg
    17281 tggtacgata ccatgctctt cattataggc catctgaatt ttccgacggc gggcagtttc
    17341 atcgatagca cgttgcatag actgggtaac cgtgtctgca tacatgatga catggccttc
    17401 actgttacga gcagcacgtc caatagtctg gatgagtcca cgttcgttgc gaaggaaacc
    17461 ttccttgtca gcatcgagaa tagctacgag gctcacttca ggaacgtcaa ttccttcacg
    17521 gagcaggtta attccgacca agacatcaaa gacacccaag cgcaggtcac ggataatctc
    17581 cgtccgttcc aaggtcttga tatccgagtg catgtacttg accttaatac ccatttcctt
    17641 gaagtagtcg gtcaagtcct ctgccatttt cttggtcaaa gttgtgataa aggtacgctc
    17701 atttttttca acgcgggcat tgatttcacc caagaggtca tcaatctgtc ccatagtcgg
    17761 acggacttcc acctctggat ccaagagtcc cgttggacga atgatttgct caatcactgt
    17821 ctcggtctgt tcattttcat agtcaccagg tgtcgctgaa acgtaaacaa tctgatgaac
    17881 gtgactctca aactcctccc gacggagagg acgattgtcc aaagcagacg gcaaacggaa
    17941 accataatta accagcattt ctttacgcga acggtctcca ttgtacatgc ccttgatttg
    18001 ccctatggtc atatgactct cgtcaatcat aatcaagaaa tcatctggga agaagtcgag
    18061 aagcgtataa ggaggctctc cttcgctccg tccatccatg tggcgagaat aattttcaac
    18121 cccattggta tagcccatct cacgcaacat ttcgatatca tactctgtcc gctgtttcaa
    18181 acgctgggct tcaagcagtt taccttcctt ttcaaagaca gctaattgtt cttccaactc
    18241 ggcctgaatc tttgcaatgg caacttccat gtggtcgtca ttggtcacaa agtgtgtcgc
    18301 tgggaaaatc gctaaatgat ccacttctcc caacacctga cctgtcagag cctcaacttc
    18361 acgaatacgg tcaatttcgt ctccaaaaaa ttctactcga aaggcatgtt catctcggga
    18421 agctgggaaa atctctacca catccccacg aacgcgaaat cttccgcgtt ggaaatcaat
    18481 atcattgcgt tcaaactgaa tatcgaccaa gtcattcaag agtttatcac gagaaatctc
    18541 tagaccagga cggagactaa cgacactatc agcgtattcc ttgggcgaac ccaaaccata
    18601 gatacaagag actgaggcca cgacgataac atcattacgc tccaaaaggg ctgaggtagc
    18661 tgagtggcga agcttgtcaa tctcgtcatt gacagaacta tccttctcaa tataggtatc
    18721 gctagaaggg acataggcct ctggctggta ataatcatag taggatacga aatactcaac
    18781 tgcattttca gggaaaaatt ccttaaactc cccatagagc tgaccagcca gagttttatt
    18841 gtgggcaata accagagttg gtttattgac tttagaaatg acctgactca tagtataggt
    18901 cttccctgtt ccagtcgccc ccatcagaat ctgagctttt tctcccccct caatgttatc
    18961 caccaactgc tcgatagctt ggggttgatc tcctgatggt tgatattttg atactagttt
    19021 aaattgatta tctgtaatgt gattgatcat aagagtcctc tctccatgtg ctattcctat
    19081 tttagcatac ttatagtatt ttcacgaaga aaaggacgga ccgaagtcac atcctttcat
    19141 tttctttctt taatttatta gcaagataga caatcagcag tagtaatacc agacttgtca
    19201 taatggaacc ttctatgcca aaagaaccac ctgatagcca atcttgattg ccttgtggta
    19261 aaaaggtcat cagagacgtt cctgacggtt gaccactaac taaaatccca aagagattac
    19321 cctgagcaaa attccaagca ccatgaatac ctgcaacacc ccaaactgta tcagttttga
    19381 gaaggtaaag agccatggca actccgaata aaaagagatt tactagagat agaggggtga
    19441 ggccagaatt gcccatatga agcagggtaa agaacaggct agatataaga atagctagtt
    19501 ttagattggt tcttgaggcc aattgaggaa gtagccaagc acgggccacc acttcttctg
    19561 ctgtcccctg taaaatccaa aatgggatag taaagacgac aaaggcaaga taataaggat
    19621 tcaagtgaat ggattccaaa cgatattgac ctaagaccac taaacttaac aaggtcagaa
    19681 gaaaaagtgc caggcctaga ctaaatcctt tcagaagatt gctgaggcaa ttctctctat
    19741 aaaatcccaa ggttcgaata ggtcttttct caacttttct tgtccatctg aacacagtgt
    19801 taagaataaa accaaaggcc agtaatccta aaattaaacg aaagtcactt gttttatccg
    19861 tcaagctctg atgcaaggtc tgcaagtagc ttgcaagatt ttgaccagcc tctccaaaat
    19921 ttctagcaag tccgatgaga gctaacaaac taataccata taaagtataa gccacaaact
    19981 ctcctatgaa gacaaaaaca aaggctaaca aggggcttgt gtaaaggttt aaattcattt
    20041 ttgaagcttg gaagtcttta aatattcttt tgttcttcat atccccgtcc tctttt
//
LOCUS       DAVKJU010000036        19973 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVKJU010000036 DAVKJU010000000
VERSION     DAVKJU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.036"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..772
                     /locus_tag="VW191_001253"
                     /pseudo
     CDS             1..772
                     /locus_tag="VW191_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(889..1236)
                     /locus_tag="VW191_001254"
     CDS             complement(889..1236)
                     /locus_tag="VW191_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9624021.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(1347..3122)
                     /gene="spxB"
                     /locus_tag="VW191_001255"
     CDS             complement(1347..3122)
                     /gene="spxB"
                     /locus_tag="VW191_001255"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEU9624022.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(3329..5572)
                     /locus_tag="VW191_001256"
     CDS             complement(3329..5572)
                     /locus_tag="VW191_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU9624023.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIRLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5582..5953)
                     /locus_tag="VW191_001257"
     CDS             complement(5582..5953)
                     /locus_tag="VW191_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU9624024.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYIVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5964..6359)
                     /locus_tag="VW191_001258"
     CDS             complement(5964..6359)
                     /locus_tag="VW191_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEU9624025.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6543..6641
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6725..7516
                     /gene="thiD"
                     /locus_tag="VW191_001259"
     CDS             6725..7516
                     /gene="thiD"
                     /locus_tag="VW191_001259"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9624026.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7726..8358)
                     /locus_tag="VW191_001260"
     CDS             complement(7726..8358)
                     /locus_tag="VW191_001260"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9624027.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(8351..9154)
                     /locus_tag="VW191_001261"
     CDS             complement(8351..9154)
                     /locus_tag="VW191_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9624028.1"
                     /translation="MQEFTNPFPIGSSSLIHCITNEISCEMLANGILALGCKPVMADD
                     SREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLVVSKNQVAVLGNGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(9154..9678)
                     /gene="thiW"
                     /locus_tag="VW191_001262"
     CDS             complement(9154..9678)
                     /gene="thiW"
                     /locus_tag="VW191_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEU9624029.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFHYSALGE
                     ILGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9683..10375)
                     /gene="tenA"
                     /locus_tag="VW191_001263"
     CDS             complement(9683..10375)
                     /gene="tenA"
                     /locus_tag="VW191_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEU9624030.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDTFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(10386..11036)
                     /locus_tag="VW191_001264"
     CDS             complement(10386..11036)
                     /locus_tag="VW191_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEU9624031.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(11038..12423)
                     /locus_tag="VW191_001265"
     CDS             complement(11038..12423)
                     /locus_tag="VW191_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624032.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAAMAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRELTSIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12424..12984)
                     /locus_tag="VW191_001266"
     CDS             complement(12424..12984)
                     /locus_tag="VW191_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9624033.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNIFSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(13246..13334)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13543..14172)
                     /locus_tag="VW191_001267"
     CDS             complement(13543..14172)
                     /locus_tag="VW191_001267"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU9624034.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(14174..14956)
                     /gene="thiM"
                     /locus_tag="VW191_001268"
     CDS             complement(14174..14956)
                     /gene="thiM"
                     /locus_tag="VW191_001268"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEU9624035.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKAEFINALYEITDEDVKEFKRVK"
     gene            15047..15133
                     /locus_tag="VW191_001269"
                     /pseudo
     CDS             15047..15133
                     /locus_tag="VW191_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(15283..15951)
                     /locus_tag="VW191_001270"
     CDS             complement(15283..15951)
                     /locus_tag="VW191_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEU9624036.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(16167..16255)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(16507..17643)
                     /gene="lctO"
                     /locus_tag="VW191_001271"
     CDS             complement(16507..17643)
                     /gene="lctO"
                     /locus_tag="VW191_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEU9624037.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(18268..19758)
                     /gene="lysS"
                     /locus_tag="VW191_001272"
     CDS             complement(18268..19758)
                     /gene="lysS"
                     /locus_tag="VW191_001272"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU9624038.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19885..>19973)
                     /locus_tag="VW191_001273"
     CDS             complement(19885..>19973)
                     /locus_tag="VW191_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEU9624039.1"
                     /translation="EPMAQQVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         5982 a         4352 c         3718 g         5921 t
ORIGIN      
        1 catttgaagg ctacagaggc aggagaattt gttacaattg atgccttatt tcaggcttat
       61 aaaaagaagt taggtcgttc ctacacacgt gatgccttct atcaactgtt gaagcgccat
      121 ggttggcgaa atattatgcc acgtccagaa catcctaaga aagcagacgc tcaaaccatt
      181 gtcgcgtcta aaaataaaat ctcaattcaa gaagaaaaga aagcgtttta aaaccagtag
      241 accttttcat aaggttcgct tgatgtacca agatgaggct ggtttcggta gaatcagtaa
      301 actgggatct tgttgggctc caataggagt aggtctacat atccatagtc actatatacg
      361 agaatttcgc tattgttatg gagctgttga tgcccataca ggcgcatcag ttttcttaat
      421 agctggtgga tgtaatactg agtggatgaa cgtcttttta gaagagcttt cacaagctta
      481 tccagatgat tattttttac tcgttataga caatgctata tggcataaat caagtacctt
      541 aaagattccg actaatattg gctttgcatt tattcctcca tacacaccag agatgaaccc
      601 cattgaacaa gtgtggaaag agattcgtaa acgtggattt aagaataaat cctttcgaac
      661 tttggaagat gtcatgaatc aactccaaga tgtcatacaa ggactggaga aggaggtgat
      721 aaagtccatc gttaatcgga gatggactag aatgcttttt gaaagcagat gagtattata
      781 tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt catattctca
      841 atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct atttcatacg
      901 ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg ttccccaatc
      961 ggttacagtt ggtccgtgta aaacctttat accaagctcg ttcaaccgtt tgtagttctg
     1021 gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt ccaaaggaac
     1081 caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag caaaaccatc
     1141 atcagcataa tctgcctttt tatccaaaat atgctccaag tcagcacaga cttggggaac
     1201 atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat aaaaagaccg
     1261 gattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag tctacaagtt
     1321 tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa tccttcttct
     1381 tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt gaaggctttg
     1441 atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg aagtggacgg
     1501 tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa tttaacagct
     1561 tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac agctccttga
     1621 gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa gtgtttgttt
     1681 gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac aaggttgata
     1741 actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt gaatgctcca
     1801 tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc gataccacca
     1861 ggaagggcaa tacccattgt cgcaaagagt ggagatgtac gccacatgtt cttaggtgtc
     1921 atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga gtagatagcg
     1981 tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa ttcaccctca
     2041 gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt aacgtttgca
     2101 cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt agctgcttga
     2161 ccagcatcac caaggattga agcgtcaaga gcatgacgtt taccaagttt gtaagggtcg
     2221 atatcgactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc agcgaatggg
     2281 aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt ggctggtttc
     2341 caaccaacac ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa agcttcaaag
     2401 tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt aatcacttca
     2461 ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt gttcaagatt
     2521 tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga gcgttcgtat
     2581 gaacctgaac cgtagtatga gttttcatcg atttcttgga aaccgaagtt tactggaatt
     2641 tcaacaacag ctggaccttt tttagaaact gcagcacggc aggcttcgtc aattactttt
     2701 ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag caataccgtt gtacattggg
     2761 ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg tgatccaagg
     2821 atcgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat caagtgagtc
     2881 gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt agcttgcata
     2941 accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaaacggat atctttgtct
     3001 tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta gattgtatct
     3061 acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt cccttgagtc
     3121 ataatgataa ctctccttca atttttttag acttggagaa tacgattaca tagaattgga
     3181 aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt ttctaaaaat
     3241 ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagtgttgt catttctgtt
     3301 ttataacaaa gaaatctagt cattactttt agtctatttt actaaaattt aacagaaggg
     3361 aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat tctgggttga
     3421 gagccagtcc aacacctgaa aagacgcctg ctgcaatcgg aattccgaca acattgtaga
     3481 taaaagccca gaaaagattg agtagaattc gatgaaaggt tttcttactc atatcaaagg
     3541 cacgcaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac tcgatggcga
     3601 tatctgttcc agctcccata gcaatcccca catctgctac actaagggca ggagcgtcat
     3661 tgatcccatc accaacaaag gctactttac ctgactgttg cagtttatgg atttcatggg
     3721 ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga tctgcaatag
     3781 cacgcgccac accagcattg tctcctgtca gcatgactgt tcggagacca cgttttttta
     3841 gctgactgat ggctagctta gcattttcct taggaatatc ttgcaaagca agcaagcctt
     3901 tgatttcatt gtcaacagct aagaacacaa ctgtcttagc ttctttttct agttcttcta
     3961 gtttatcttg ataagtatta gaaatatcca tgccatccag cattttagca tttccaagta
     4021 aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggtc tgaaaatttt
     4081 caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc tcagccagtg
     4141 ggtgttgaga agcatcttcc aaggaggctg ccaacccaaa cacttctact tcgtcgccga
     4201 tgatatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca aagacaaggg
     4261 tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc cccatcttgg
     4321 cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag gcacaaggac
     4381 aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta gctccaagca
     4441 cgaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt cctaaaatga
     4501 caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc ggcgcacgac
     4561 ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct gagccaactt
     4621 tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg acagtatctc
     4681 caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca atactagaga
     4741 caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact cgaatcaggt
     4801 cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc aagacttctg
     4861 cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt tttctcattt
     4921 tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca ctttcgaagt
     4981 aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact agagttccca
     5041 gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa gcactctgga
     5101 tatatggctt acctgcaact aacataatag gcgttgttgc taaaaaggtt ccccaatgca
     5161 tgacttgatg actaatgcta cctgtcaaca tcccaatcat gagaatcaca agaggcacag
     5221 taaagatact agtaatccaa aaacgttgca ggagagatag agattttcga gtcttctcaa
     5281 cgactgtata gcttcccttt tgcatcttca tgccacaaga aaattcatgt cgccctaatt
     5341 cttgaggcgt aaaacgaatg actttctcct catctacgcc gattggttcc aagatacctt
     5401 cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga aaggtaatct
     5461 cagctggaat tcccttttga agccggatat gggctggatg atagcctttt tcagctcgga
     5521 tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc atagtctcca
     5581 cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag ccaaactctc
     5641 cagcctgttc aggcgtgatt tccactatat actcttcccc cattggcagg ttcgcatgta
     5701 caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatcctta cggtcaaaga
     5761 caatgcgggc tggcactgat ttcttgagga caatcaactc aggagtatag cctcccatga
     5821 cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca gatttttcag
     5881 gctttttgaa aaaccaaaac aagataaatg cgataagggc aatacaaata atggttacaa
     5941 tactatttaa catgacgtct cctttacata caattacatc ttacttctgt tacagcactt
     6001 gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt aaattcacat
     6061 tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt gtctttgata
     6121 tcttggacaa gtaaatcccg actttggtct agagttaaaa gggctgaata aacaaaggac
     6181 ttgccttctt ttttccgagt caaacactct ttctcaacca gacgagccaa aagtgtctga
     6241 accgtggact tggaccagtc aaaccgctct gccaaaaccc taatcaaatc tgtactggtc
     6301 tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga aatctgcatt
     6361 accatacctc caaaatctac atttgtcaat tacactcatc agtatactct taaaatctac
     6421 atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc atttgaaaaa
     6481 cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta aagaaaaaag
     6541 aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc ctctgactca
     6601 atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca gtcttctatt
     6661 gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg ttgggaggtt
     6721 ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta gtggtggtgc
     6781 tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa tggctgttgt
     6841 aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg tttctcctca
     6901 aatgttgaaa gcccaattgg agagtatctt ttctgatatt ccacctcagg ctgtaaaaac
     6961 tggaatgttg gctactactg aaatcatgga aatcatccaa ccctatctta aaaaactgga
     7021 ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct tgattgactc
     7081 aagtgctaga gactatctca aaacaaactt actacctcta gcaactatta ttacgccaaa
     7141 tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag acatgcagcg
     7201 tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca aaggcggaca
     7261 tctcaaaggt ggtgctaaag attttctctt taccaagaat gaacaatttg tctgggaaag
     7321 cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg cagtgattac
     7381 tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca aggcctttat
     7441 cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag tcaaccatac
     7501 aacttttaaa gattaagaaa aaaaactctc tagttcccac tttaagggaa ttagagagtt
     7561 tttatactct tcgaaaatct cttcaaacta cgtcagcttc catctgcagc ctcaaaacac
     7621 tgttttgagc tgacttcgtc agtcttatct aaaacctcaa ggcagtactt tgagcaacct
     7681 gcgactagct ttctagttta ctctttgatt ttcattgagt attaattagg aaagaatgtt
     7741 atgcaacttt tttaaaaagg cttgcgtttt tgcctcaata tcttctgctt gcatcaaatc
     7801 acgtacaaca gctacaccag ctatgccagt gcccataagc tgatcaatat tctccgaagt
     7861 caagcctcca atagcaacta ctggaatggc aaccgtttgg caaattgttt tcaaggtcga
     7921 tatcagagta atgggcgcat tttccttggt ggtggttggg aaaatggctc ctgtacccaa
     7981 gtaatctgca cctgatttct ccgcttccag agctctttta accgttttag cggtgacacc
     8041 gaggattttt tcaggaccca agactttgcg agctaccgaa actggtaatt catcatctcc
     8101 gatatgcaga cctgctgcat caaccgcaag acaaacatcc aaccgatcat cgattatcaa
     8161 gggtacctga taagcatctg ttatttcctt gacttgtttt gccagttgat aatattgatt
     8221 ggttgtgaga tttttttctc gcaattggac tatggtaacc cctgaacggc aggccgtctc
     8281 aacttttgca agaaagcttt ccacggaatc ttgatagcga ttggttacca gatatagtct
     8341 aagtgcttct ctattcataa acctctcctt tgatggtatc tagccaattt tcatctcttc
     8401 ttaggagcga aagctgattg agtacttggt aacgaaattc ttccaatccc attccttgaa
     8461 caactatttt ctcagcagcg atattgagat aagagactgc taagcaagaa gcttcaaaac
     8521 cagtctttcc ttggctgaga aaaacagctg ttaaggctcc aaccaagtct cctgtccctg
     8581 ttatccagtc taattcagta cagccatttc ccagtacagc gacctgattt ttcgaaacga
     8641 cgaggtcctt gggacctgtg actaagaaag acataccagg ataggtctga caccagtctt
     8701 tcaagacttg aagcaaatcc tccgtttctt gatctttagc actcgcatcg accccaacgc
     8761 cgtggtgctt taatccaaca agacttcgaa tttctgacat gtttccttta aggaccgtag
     8821 gtctatagtc taaaaggtct ttaactaagc tcttacgaat ggatgaagtc gttacgccaa
     8881 ccgcatctac taccatcggg agagaagatt ggtttgcata cgaagctgcc atgcggattg
     8941 ctttttcctt ctcagctgac aaatgcccca aattgatgaa gagagcctga ctttgcttag
     9001 taaaatcaag aacttcacgg gaatcatctg ccatgacagg tttgcatccc agagccaaaa
     9061 tcccatttgc cagcatctca caagaaatct cattggtaat gcagtgaatg agggaactag
     9121 agcctatagg aaagggattt gtaaattcct gcatcagtct atcctttcac taaagaaata
     9181 tccctgcact tttttaaaga attcctgctt gattaaaaat cgaaaggcaa taaaggaaat
     9241 cgctgtacca atcaaggttg ctccgaaaaa tcgaggcgtg tagataaacc agctaagctt
     9301 agcagctgat cctgtaaaga gtaccataac aggataggaa acaatggaac caataatacc
     9361 tgttcccaaa atctctccta gagcagaata gtgaaatttt cgaccgtact tataaaagag
     9421 acctgctaga agggctccaa aagtcgctcc tgtgagagct aaaggcggaa tcccttgagt
     9481 cgtcatacgg ataaaggctg tgactgtagc catagccaag gcataaacag gtcccatcat
     9541 gattcctgct agaatattga ctacactgga catcggtgcc attccctcaa ttcgaaagat
     9601 aggtgtaagg actacatcaa gggcaatcat catagataaa atggttaatt tgtgaacttg
     9661 taattggtgc tttctcatgc ttctattctt ctcctttttc taaagactgt aaatcgctct
     9721 tccatgtctg gtgttggtag gccatttccc aaaacttggc ttccatatga acactgatgt
     9781 ggaaggtatc tagcattttt tgcttgtctg tctcgtcact ttctcgatag agctgattga
     9841 ccagtgctcc ctcctctctg atctgttgct ctaactcatc cgtaatataa gtttcaatcc
     9901 attgttgata gagaggattt ggtgatggtt taagattaag tgatttgcct atatcatggt
     9961 ataaccaagg acaaggaagc aagcttgcaa aagcgatggc taagttcggt tctgcaaatt
    10021 gcctataaat atgagaaatg taatgataac aggttggagc gattggatgt tgctccattt
    10081 cctggtcgct gatttccaat tccttgaaaa attgttggcg aataaataac tcaccctcca
    10141 ctaaaccctg agcattttgt ttcaagagtc ttttcatctc ttggtttgaa gtcttatcag
    10201 ccaaaagatg atagatttct gagaaggcct tcagatagta ggcatcctga atcaggtaat
    10261 agcggaaaat ggcaggttct aaattcccct cttgtaattg taaaataaag ggatgatgaa
    10321 aggaagcctg ccaagctttc ttggataatt ccatcgcaat atctgtaaat tccataataa
    10381 ctcctttata aaaatagact ggtttgaagc aataaaaaga aaagcaggta gattaatttt
    10441 gtttttttag gaatataaaa agtccgatag ctattcttca actgtgcatg ttcgtcatat
    10501 ccatgagcag acagagctct caggtaaaga tggcgccacc taaagactgt catcagaacc
    10561 ttactgtaaa tcaagggcga ccaaaaatgc agttcttgac cacgtaatag gcaagcttct
    10621 ttgagggact tgatttcttg ctgaatgaga ggaaaggaat tgaataccac aatcaaggca
    10681 taggaccaag agcgtgatag ccccttttga gccaagtaca agagaagctc ttttagtgaa
    10741 atagaggaaa caaagacaag gccgatacaa actgtcacaa aggccctcgt tccaagcatg
    10801 actgcctgtg aagcatctcc gtgtaactga actgcccagt agttggcaaa agatggtaaa
    10861 atggcaagta tgatcatcca agctaacatt ttaaatcgac ggtaatagag cataaagaga
    10921 atacaaaatg cgactaccga aagattcaga gcaatcgaag gaatgaaaga tgtttccaag
    10981 gataaaatca gcaagaagag actgataatc ggtgtctggg ttgctacttt gaccatacta
    11041 tctcacctcc ccttgggtat tgctactctg agatgtaagt ggtttggtaa tggtcacttc
    11101 tttcacatgc cgaagaccct gactagtcat ctcaatccaa taatcaacca cagaaatcaa
    11161 agggtctaaa cgatgactaa tgagcagaaa acttcttcct tgattcctct cctccacaat
    11221 ccacttgcaa aaataatggc aggctctatc atccaaacct gcaaaaggtt catctagcaa
    11281 gatcacggaa gccttactgg tcaagatggt caagagctga agaatttttt gctgaccacc
    11341 acttaattga tagggactct tatcgactgc ctgctccaaa tcaaaatatc gtaaagcttg
    11401 aaaaatccgc tgatttcttt cagaatcagg tccatctaat tgaagctcct ctcgcagact
    11461 gactcggata aactgcttct cagcttcctg aacaatactc gtcagttcac ggtacaaact
    11521 ctttttcttt ttcaggactg tccccttcca tgcaatgcgc cccttatact tttgaaattg
    11581 aagaatagac cgaaagaggg ttgatttccc aacaccattg tcacccagga tacaggaaat
    11641 tccttggtaa aatgtaaaat cagcaatcga aaagagggag cgatttccaa actcacaagt
    11701 cgttctatcc atatggaata gttccgggct agaagcaact tcctttgaag caacctgtgt
    11761 catctcatag gaagggattt gaaacacttc ccttagtttt ccgtctctta gctcaaccat
    11821 atggtcgata taggctttat agtcagataa atcatggtcg cacaaaataa ctgtcttccc
    11881 atcaagagcc aactctttta gaatctccaa tatctcgatt ctgctcttgc ggtcaatgga
    11941 agcgaagggc tcatccaaga gatataccct aggattcatg gcaaagagga cagccatcgc
    12001 tgctttttgc ttttccccac ctgataggtg atgaatggga cggtgtaaga ttgcctcgca
    12061 acgacattgc tggacaacct ctgctatttt agaatcaatt tcctgaaggt gatagccgat
    12121 attctccaag gtgaaaatca gctcctcaaa caagttctcc atggtaaatt gatgattggg
    12181 attttgaaag agaatgccaa ccttctggac acgtgcgatg atagaaagct gactgacctc
    12241 gctcccatct atcaggactt gaccactata gggaagagaa ctgacttggg caatgacttg
    12301 aaaaaggctg gattttcctg aaccactgct cccaactaac aaggtaaagg cttgcgcatg
    12361 aaaagtaaaa tcaaacggct cagagaagat tggggactga atcgctcgta gttccagacc
    12421 catctatgct tttcctccag ttgcaaactg atgatagagt ttgacaatgg cacgaaccaa
    12481 gatggtacag aagaaataaa cagaaataaa acgtaccaca agtaaggaaa ggacaaacgg
    12541 aagggaaaag gcgtagtaac ctaacttaat gtattcatag acaaagctaa caagcgtaat
    12601 cccaatacta ttagcagtta gagagagcca actttcatag cgattcttag ttacgataaa
    12661 accaaattca cttcccaaac cttgaaccaa gccagacaaa agagctccta gaccaaattg
    12721 gctaccataa aggacttcag caagcgcagc tagcacttct ccaatcgttg cacttccgac
    12781 tctcggaaca aagatggcag caatgggcgc agccatacac cagagaccga agaggatttc
    12841 attggcaaag gcctgcaaac caagaggtgt taagagtaga ctgaaaatat tatacacata
    12901 tcctgaacca acgaaaaccc caccaaaaaa gatagacaag aaagcaagca agataacatc
    12961 ttttaactgc cattttttca acataaaaaa ctcctttttt aaaagaaaag tggggcactc
    13021 aagaagaccg acctaaatac tttgtatagc agactgaatt tagaacagta cacaagaatg
    13081 ctaaaatatt tctagaaatt aatttgaatt ttctaattga tttgttcgca tcttatttca
    13141 atctactata tcatcttcat ccagtttcgt aaaagaaaaa actctaatta cagatacgaa
    13201 ttagagttca gcttacaaga ttagacagtt cttttcgaca tacgaaaaaa acatttcaca
    13261 tttcccttcg ccagtcttaa ctgtatcagg ttcaatgggt atcatctcag cctaaagcac
    13321 cccaaatgtc tttattattt aattatgtga ttattataac acacatttta tactagttca
    13381 agaaattgaa ctggaaatac agccttgcac tcacaaagac agcagatctt tcttttgcaa
    13441 aaaacaaatg acctgtttga tgaattagcc attcaagctg aatctggaca tagcttttta
    13501 aaaaaggaaa atcctactta cttagaatcc aaggatagat atctattgtt cactcatttc
    13561 ccgaacagtt ttttctatat tttttgcata cgatattgcc gaaatgattg aaacgccatc
    13621 catattggtc tttataatgt ctttaatatg tttcgtctgt atcccaccaa ttgcaactaa
    13681 aggcatttgt ggcaatagtt ttctcatcaa ttcaagacct tcataaccta tagcaccacc
    13741 agcatcatct tttgactggg tatcaaatac aggcccaaca cctacataat ctacatattc
    13801 aacttttgat tgttgaaatt cttcttcgtt tcttatagaa agaccaatta ttttatctgg
    13861 catcaatttt ctaatttcat caacaccaat atcatcttga cctacatgta cgccatcggc
    13921 gtcaatttcc attgctaaat ctatatcgtc attaacgata aatggaacat tgtatttttt
    13981 acaaagttct ttaatttgga tagctagctc aagtttttct aagccttcta aagcaccctc
    14041 acctttttct cgaaattgaa ataaggttat accacctttt aaggcttcct caacgactgt
    14101 atatagattt tttccttggc aagtagtcgt tccacaaata aaatatagtt ttagtaattc
    14161 tttatgaaac atcttacttc actcttttga attcctttac atcttcatct gtaatctcgt
    14221 ataaggcatt tataaattca gctttaaatg ttccaggaag atgtccattg ggacgttttt
    14281 ctgccatttc tccagcgata ttgtaaacta acattgctgt ttctaatgat tttaattctt
    14341 gacctttttc cagtccgata aaacttgcta ctacagctcc taataagcat cctgtcccaa
    14401 tgactttcgg catcatagca ctaccattat gaatcgttac cacttctcca ttaacagcaa
    14461 tggcatccac ttcacctgtt actactattg gaatattgaa cttctcattt gctgctagag
    14521 caatttcgtc aatattatct acgcccgcac tatctactcc tttagatgcc acatctattc
    14581 ctactaaaga ggcaatctcg ccagcatttc ctctaatcgc tgctagttta taattgttga
    14641 ttagatcatc tgctactttt tttctatatt ctcctgctcc acaggctaca ggatctaaaa
    14701 ctgctgggac attatatttc tccgcaattt tcagagcagc ttggtataat ttccaatttt
    14761 catctgtcaa tgttcctatg tttattaata aaccaccagc atactttaac aaatcctcta
    14821 aatctgctgg aaactcactc atggctggtg aggcgcccag tgctactaat ccatttgctg
    14881 tgaaattttt tactacatca ttggttatac aaatgaccaa tggtgctttt tcttttaata
    14941 attttaaact tgtcatattg aaatccttcc ttttcacttt atacgatcta ctgatttcga
    15001 tttatctcta tctttcgtta agaaattttt ttcatttacg ttgaatgatt tatactcaat
    15061 gaaaatcaaa gagcaaacta ggaagctaac cgcagattgc tcaaaacact gctttgaacc
    15121 tgtagataag actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga
    15181 cgaagtcagt aaccatacct acggcaaggt gaaactgacg tggtttgaag agattttcga
    15241 agagtcttac ctcatcaaat ttgtaaatat catgagcctt ctctagacat cgtaaccaat
    15301 atcaaaaaaa gctaattcta aagcgactgc ttgattccag cgttgctgaa gttctgtcaa
    15361 atcttctcga tttttaccga cacgattgag ttcgtcaacc agaaattgaa cccactctgc
    15421 aaagaaagga cctctgtgga gattgatcca ttccgaatga atatagactt caggtagagc
    15481 caaatcttta gaaccccagt ctaaatagag accttctgca atgaccagca taaccaaaag
    15541 atgggcatag tctgatgaag ccaccgccga atacattaga tcctgaaagg cttttgttac
    15601 agggtgcaaa gtcacttcta ggtagtcatt ctctgctact tttaactctt taaaagcctt
    15661 ttggaaataa ccatcttcat ctgcttcaag aaagcctagt tgcttggcaa aacgaagctt
    15721 ggattcaagt ttatctgcgt gagctacgca ggcacccagc atggataaga aggcatcaaa
    15781 gaagtgataa tcttgaatca gatagtcctt taagacctta ttctcaattg tccccgcaaa
    15841 aagttcctta acaaaacgat gattgattgc agcctgccaa tccttctgac tgctttttaa
    15901 taattctcca acagtcaaac ctggctgaaa tgcatagtct tgtgtttcca tatttacttc
    15961 tcctctcttt acttgttagt aattaataaa acaccaagaa atatcaagca aaatcgtaat
    16021 tccacttgat ccttttaaag cacatcgaga gcatttgcag agaagctaac taaacaagcc
    16081 tatccagttt atataaacaa aaaactccaa ttacaatcaa gaattagagt tgacttacaa
    16141 gattagatcg ttcatttcgc catacgaaaa aacgtttcac atttcccttc gccagtctta
    16201 actgtatcag gttcaatggg tatcatctca gcttaaagca ccccaaatgt ctctattatt
    16261 taattactga accagtatag caaaaaatga aagccctagc aagatatttg accgaaaaat
    16321 atctttatat agtagattga aactagaata gtacacctct acttctaaaa cattgttaga
    16381 aatcgatttg actgtcctga ttgatttgtc ctattcttat ttcattttac tatagttttc
    16441 gatagcaatt tattcttcca atacacgaag aaaaacctcc acattcagtg gaggcaatct
    16501 gttttatcaa tacaatttta agtcacgagg gtcaactggg aaggttgggt tgtatggatt
    16561 gtgacggagc ttaaagtgtt tgacatcttc aatggtctga gctccagaca attgcataac
    16621 tgtcttcaat tccgcattca agtgttcaaa gacttgacgc acaccgacac taccaccgag
    16681 agccaagcca taaataacag ggcgaccaat agctaccaag tctgctcctg aagccaaggc
    16741 tttaaagacg tgttgaccac gacgaacacc agagtcaaag acaatcggca cacgtctatc
    16801 aactgcttct gccacttctt gaagcgagtc aaaggcagct ggtccaccgt cgatttgacg
    16861 accaccgtgg ttggttaccc agataccaga agctcctgca gcaagtgaac gttcaacgtc
    16921 ctcacggcat tgtggtccct tgacatacac aggaagacca gagtattcag cgataaattc
    16981 tacatcgcgt ggagacaagc gttgtttagc tgatttgtaa acaaagtcca ttgatttacc
    17041 agcaccttct ggcaggtatt cttcaacaat cggcatgcca actgggaaga caaaaccatt
    17101 acgcttatcc acttcacgat tcccccctac agtagcatct gccgtcaaga caatcgcttt
    17161 ataaccttca gccttcacac ggtccatgat gtggcggttg ataccgtcat ccttactaaa
    17221 gtaaaattgg aaccaatggg gtgtcccttg aagggcttca gaaatctctg gaaggtcaac
    17281 agtagagtaa gagctggttg tataaagaga accaaactca tgcacaccac gcgcagtcgc
    17341 cacttccccc tgttcatttg ccaatttatg agccgcaaca ggcgccataa tgattggtga
    17401 agatagtttt tcacctgcaa attcaatctc tgtacttgga ttttcaacat cgcaaagtgt
    17461 atgaggaacg atgagtttgt ggttaaaggc gcggatattc tctcttaaag tgaaagtatc
    17521 ttccgcccca ctagcgatat agccaaatgc tgctttagga ataacttgtt gcgccattgg
    17581 ctccaaatca taggtattga tgaagtctac atgaccttct gcattgcttg ttttgtatga
    17641 cataaaatgc cctccttgat taagtaagcg tttgcttttt actacacaac tataataact
    17701 cttttttcaa tcattttcaa gagttttgct taaaaaccat cttatttttt catacaatat
    17761 actagcccta ctttccttaa actctaaact ttagtctata ttaagtgaat cgttgagatt
    17821 ttttgctgcc aggcatttga actacttttt tatttgcgga ttctatctac taatactata
    17881 aaaaaacaag tgaataagac accagaacat cctctaatag gacctatgga cttatatcag
    17941 tcccatatat actgtttaag caagatacta tcctagttga cgctaacttc ctatcccact
    18001 tattaatttt ttatttatag tgaatctgaa caaatcaatt ggggaatcca agtcaatttc
    18061 tagtactttt tgaacagttt cagcgtacta tcgtaaaatg aaataaaaca tgcacaaatt
    18121 gcttagggaa tttaaaccaa tttctaacaa tgtcttagaa atcaaagtgt actatttcaa
    18181 cttcaatata ctacacttca ttttctcctt aaattcagtc tctttttgaa tcaaaaaatc
    18241 ccctggtaag agccagagga taagaattta tttcattgtt gggaagagca atacatcacg
    18301 gatagttgtt gtatcagtga ggagcatgca gagacggtcg ataccgattc ccaaaccacc
    18361 tgttggtggc ataccgtatt caagagcttc aatgtagtca tagtcgattc ctgtcgcttc
    18421 atcatcacca agttctttgg ctttagcttg ggcttcaaaa cggctaagtt ggtcgattgg
    18481 gtcgttcaac tcagtaaagg cattaccgta ctccttagtc atgataaaga gctcgaaacg
    18541 gtcagtaaag cgttggtctt caggattttt cttagcgagt ggagatacag ctactggatg
    18601 tccatagaca aaggttggtt ggattaaagt ttcttcaaca aactcttcaa agaaggcatt
    18661 gatgatgtga ccaacctcag tgtagtgttt ctcaactgga actttcttct cagcagcgat
    18721 agctttagct tcttctaaag tcatgtcttg ccagaaatcg acaccagtaa tttctctgat
    18781 agcatccacc atatgaacac gcttaaatgg ttcgttaatc ttgatttcag taccttggta
    18841 gttgactggg ccatcgccct taactgattt agcagcgtgt tggataatgc cttccgtcaa
    18901 gtccatgatg tcttggaagt ctgcataagc ttggtaaact tcgatagaag taaactcagg
    18961 gttatgagta gcgtccattc cttcgttacg gaagatacgg ccaatttcat agacacgttc
    19021 cataccaccc acgataaggc gttttaagtg aagctcagtc gcgatacgaa gcaccatgtc
    19081 aatgttttgg gcattgtggt gggtgataaa tggacgggca gcagcaccac cggcttcatt
    19141 atgaagaaca ggtgtttcca cttcaaggaa tcctttttgg tcaaggtaac gacggatttc
    19201 agagatgatt tttgaacgag tgacaaagcg ttcaaagctt tcacggttag aaatcaagtc
    19261 aaggtaacgt ttacggtaaa ttgtttcaac gtctgtcaaa ccatggaatt tctcaggaag
    19321 aggacgaaga gccttagaca agtgtgtgat gtgggttgcc ttgatagaga gttctcccat
    19381 atccgtacgc atcacttcac cttcgacacc aaggaagtca ccaaggtctg cttttttgaa
    19441 gatttcgtag ttttcttcac cgacagcatc cttacgaacg tagatctgaa tctggccttc
    19501 gcggtcttga aggtgggcaa aaccaacttt tcctttacca cgtttggtta ccaagcgtcc
    19561 tgcgatagta gctgtttcgt ttttatcgtg taattgttct ttatcgaggt tggcatattt
    19621 atcttttaat tcttgtgaat ttgcagtacg ttcaaaacgt tttccgaaag gatcgattcc
    19681 ttgttcgcgg agcgcagcca ttttttcacg gcgaacgatc tgctggtcat ttagttcttc
    19741 catatgttct gtagacatgg gatcctccta gttttactca aaattgaata cgatgagtca
    19801 ttttttgcga taccccattt tatcataaat aagcaagaaa ttcacggata atctttaaaa
    19861 actaaaaaga acctgacgct aaaatcactg gaaagaagtg aaagtatctt ccgccccact
    19921 agcgatatag ccaaatgctg ctttaggaat aacttgttgc gccattggct cca
//
LOCUS       DAVKJU010000037        19821 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVKJU010000037 DAVKJU010000000
VERSION     DAVKJU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.037"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            117..3218
                     /locus_tag="VW191_001274"
     CDS             117..3218
                     /locus_tag="VW191_001274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU9624040.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLIGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            3301..4308
                     /gene="pfkA"
                     /locus_tag="VW191_001275"
     CDS             3301..4308
                     /gene="pfkA"
                     /locus_tag="VW191_001275"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEU9624041.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEVIIPEADFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTKLGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4367..5872
                     /gene="pyk"
                     /locus_tag="VW191_001276"
     CDS             4367..5872
                     /gene="pyk"
                     /locus_tag="VW191_001276"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU9624042.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6535..6757)
                     /locus_tag="VW191_001277"
                     /pseudo
     CDS             complement(6535..6757)
                     /locus_tag="VW191_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7605..8477
                     /locus_tag="VW191_001278"
     CDS             7605..8477
                     /locus_tag="VW191_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEU9624043.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8626..8967)
                     /locus_tag="VW191_001279"
                     /pseudo
     CDS             complement(8626..8967)
                     /locus_tag="VW191_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9110..9421
                     /locus_tag="VW191_001280"
     CDS             9110..9421
                     /locus_tag="VW191_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624044.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            9425..10141
                     /locus_tag="VW191_001281"
     CDS             9425..10141
                     /locus_tag="VW191_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU9624045.1"
                     /translation="MKKYFIGGLGNNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10271..11086)
                     /locus_tag="VW191_001282"
     CDS             complement(10271..11086)
                     /locus_tag="VW191_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEU9624046.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11083..11988)
                     /locus_tag="VW191_001283"
     CDS             complement(11083..11988)
                     /locus_tag="VW191_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEU9624047.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMFFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            12400..14529
                     /locus_tag="VW191_001284"
     CDS             12400..14529
                     /locus_tag="VW191_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEU9624048.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14516..14954
                     /locus_tag="VW191_001285"
                     /pseudo
     CDS             14516..14954
                     /locus_tag="VW191_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
     gene            15012..15284
                     /locus_tag="VW191_001286"
     CDS             15012..15284
                     /locus_tag="VW191_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9624049.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15638..15829)
                     /locus_tag="VW191_001287"
     CDS             complement(15638..15829)
                     /locus_tag="VW191_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624050.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16257..17015
                     /locus_tag="VW191_001288"
     CDS             16257..17015
                     /locus_tag="VW191_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624051.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            17017..19005
                     /locus_tag="VW191_001289"
     CDS             17017..19005
                     /locus_tag="VW191_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9624052.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSAFFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTIHKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19047..19691)
                     /locus_tag="VW191_001290"
     CDS             complement(19047..19691)
                     /locus_tag="VW191_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEU9624053.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYASYIQNGEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19717..>19821)
                     /locus_tag="VW191_001291"
     CDS             complement(19717..>19821)
                     /locus_tag="VW191_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9624054.1"
                     /translation="NNYPKKCLDYKSLREDFWMANLNLKFSVRNKSRN"
BASE COUNT         6092 a         3603 c         4201 g         5925 t
ORIGIN      
        1 caaaaataca aatgaataga aaataaatta gtaagctgat gaaatttttc tcaagagaag
       61 ccatttatag gtgaaaatgg tataatatag tgagaaggat agaggagaag tgtaaattga
      121 tcgcacaact agatacaaaa acagtctata gttttatgga aagcgtcatt tcgatcgaaa
      181 agtatgtgag agcagctaaa gaatacggct acactcattt ggctatgatg gatattgaca
      241 atctttatgg cgctttcgac tttctagaga ttacaaaaaa atacggcatt catcctttga
      301 tagggcttga aatgacagtg tttgtagatg atcaggaagt gaatttgcgc tttttagctc
      361 tatctagtgt gggctatcag cagttgatga agctttcgac agccaagatg cagggggaga
      421 aaacttggtc agtcctgtcc cagtacctgg aggatatcgc ggtcattgtg ccttattttg
      481 atagagttga gtcgttagaa ctaggctgtg attactatat aggggtttat ccagaaacac
      541 tagcaagcga atttcatcat cctatcttac ctctttatcg ggtcaacgct tttgaaagca
      601 gggatagaga agttcttcaa gttttaacag cgattaaaga aaatctaccg ctcagagaag
      661 ttcccttgcg ttcgagacaa gatgtcttta tatcagcaag ttctttagag aaactattcc
      721 aagagtgttt tccgcaagct ttggacaatt tagaaaagct tatttcaggc atttcttacg
      781 acttggatac tagtctgaaa ctgcctcgtt ttaatccagc tagaccagca gtagaggagt
      841 tgagagagcg tgctgaactg gggcttgttc agaaggggtt gactagtaaa gaatatcaag
      901 atagactaga ccaagaattg tctgttattc atgatatggg ctttgatgat tatttcttgg
      961 ttgtttggga tttgttgcgt tttggacgat cgaatggcta ttatatggga atgggaaggg
     1021 gttctgcagt aggcagtttg gtttcttatg ccttagacat cacggggatt gacccagtag
     1081 agaaaaatct gatttttgaa cgctttctta atcgtgaacg ctataccatg cctgatattg
     1141 atattgatat cccagatatt tatcgtccag attttatcag atatgttggt aataaatatg
     1201 gtagtaaaca tgcggcacaa atcgttactt tttcaacctt tggagccaag caagctcttc
     1261 gagatgtctt gaaacgcttt ggtgtgccag agtatgaatt atctgcaatt actaagaaaa
     1321 tcagttttcg tgacaatctt aagtcggcct atgagggcaa tctccagttt cgtcagcaaa
     1381 tcaatagtaa gttagaatac caaaaagctt ttgagattgc ttgcaagata gagggctatc
     1441 caaggcaaac ctctgtccat gcggctggtg ttgtaattag tgaccaagat ttaaccaact
     1501 acattcctct aaagtatggt gatgaaattc cactgactca gtatgatgct catggagttg
     1561 aggctagcgg acttttgaag atggactttc tgggactacg aaatttgacc tttgtccaga
     1621 agatgcaaga gttgcttgct gaaacagaag gtattcatct gaaaattgaa gaaatcgatt
     1681 tagaagacaa agaaacgtta gctttatttg cctctggtaa tacaaaaggt atctttcaat
     1741 ttgagcaacc aggtgccatt cgtctgctta agcgtgtgca accagtctgt tttgaagatg
     1801 tcgtcgcgac tacttctcta aatcgaccgg gtgctagtga ctatatcaat aattttgtgg
     1861 caagaaagca tgggcaggaa gaagtgactg ttctggatcc agtactggag gatattttgg
     1921 ctccaaccta cggcataatg ctctatcagg agcaggttat gcaggttgcc cagcgacttg
     1981 ccggatttag tcttgggaaa gccgatattt tgcgtcgggc tatggggaaa aaggatgcct
     2041 ctgccatgca tgagatgagg gcttccttta ttcaaggttc attagaagct ggtcatactg
     2101 tggaaaaagc agagcaggtc tttgatgtta tggagaagtt tgcaggctat ggttttaaca
     2161 ggtcacacgc ctatgcctac tcagcattgg ccttccagtt ggcttatttt aaaacacatt
     2221 atccagccat attttatcag attatgttga attctgccaa cagtgattac ttaatagatg
     2281 cacttgaagc aggttttgaa gtagcctctc tatccatcaa caccattccc tatcacgata
     2341 aaattgccaa caaggccatc tatctaggtt tgaaatccat taaaggagtc agtaatgatt
     2401 tagctctctg gattattgaa aatagacctt attctaacat tgaagatttt atagctaaat
     2461 tacctgagaa ttatctgaaa cttcctctgc tagaaccttt ggtaaaagtt ggtcttttcg
     2521 attcatttga aaaaaatcgt caaaaagtat ttaataactt agctaatcta tttgaatttg
     2581 tgaaagagtt gggaagtttg tttggagatg ctatttatag ttggcaggaa tcggaagatt
     2641 ggacggaaca agaaaaattt tatatggaac aagagctttt agggataggt gtcagcaaac
     2701 atccactaca agctattgca agtaaggcta tttacccgat taccccaatc ggaaatttgt
     2761 cagaaaatag ctatgctatt attttggttg aagttcagaa aataaaagtg attcgtacca
     2821 aaaagggtga aaatatggcc ttcttacagg cagatgatag taagaaaaaa ttggatgtca
     2881 ctctcttttc agacttatat cgtcaggttg gacaggaaat aaaagaggga gccttctact
     2941 atgtaaaagg aaaaatacaa tcacgtgatg gccgtctgca aatgattgca caagaaataa
     3001 gagaagcagt tgctgaacgc ttttggatac aggtgaaaaa tcatgaatcg gatcaagaaa
     3061 tttcacgcat tttagaacaa tttaaaggcc caatcccagt catcatccgg tatgaagagg
     3121 aacagaaaac catcgtttct ccccatcatt ttgtagctaa atccaatgaa ttagaggaga
     3181 aattgaatga aatcgttatg aaaacgattt atcgctaaaa atacggaaaa tagaagaatt
     3241 ttaaaatcaa atgtgatata atcaagaaga atgttaaaag aaaaaggagc ataaaccaat
     3301 atgaaacgta ttgctgtttt gactagtggc ggagacgcac ctggtatgaa cgctgccatc
     3361 cgtgcagttg ttcgtcaagc aatttcagaa ggaatggaag tgtttggtat ctatgacgga
     3421 tatgctggta tggttgccgg tgaaattcat cccctagatg cagcttcagt aggggacatc
     3481 atttctcgtg gtggtacttt tcttcactca gctcgttatc cagagttcgc tcaacttgaa
     3541 gggcaactta aagggattga gcaattgaaa aaacacggaa ttgaaggtgt agttgttatc
     3601 ggtggtgacg gatcttacca cggcgctatg cgtttgactg aacatggctt cccagctatt
     3661 ggtcttccag gtacaatcga taacgatatc gttggtactg actttacaat cggttttgac
     3721 acagcggtta ctactgccat ggacgctatt gataagattc gtgatacatc atcaagtcac
     3781 cgtcgtactt ttgtaatcga agttatggga cgtaacgctg gtgatatcgc tctttgggct
     3841 ggtattgcaa ctggtgctga tgaagtcatc atccctgaag cagacttcaa gatggaagat
     3901 atcgtagcaa gcatcaaagc tggttatgaa tgtggtaaaa aacacaatat catcgtctta
     3961 gctgaaggtg tgatgtcagc ggctgaattt ggtcaaaaac ttaaagaagc tggagataca
     4021 agcgaccttc gtgtaacaaa acttggacat attcaacgtg gtggttctcc aactgcgcgt
     4081 gaccgtgttt tggcgtcacg tatgggtgca catgctgtta aacttcttaa agaaggtatc
     4141 ggtggtgttg cggttggtat tcgtaacgaa aaaatggttg aaaatccaat tcttggtact
     4201 gcagaagaag gggcattgtt tagccttact gcagaaggta agattgtggt taacaaccca
     4261 cacaaagccg atattgagct atctagcttg aataagagct tgtcataatt tacaatttag
     4321 ttaataagac caaaaaggtc atatatataa aggagtcaca aaaatcatga acaaacgtgt
     4381 aaaaatcgtt gcaactttgg gtcctgcggt agaaatccgt ggtggtaaaa aattcggtga
     4441 ggacggatac tggggtgaaa aacttgatgt tgaagcttca gctaaaaaca ttgctaaatt
     4501 gattgaagct ggtgctaaca cattccgatt caacttctca cacggcgacc accaagaaca
     4561 aggtgagcgt atggcaactg ttaaacttgc ggaaaaaatt gcaggtaaaa aagttggttt
     4621 ccttcttgat acaaaaggac ctgaaatccg tacagaattg ttcgaaggtg aagctaaaga
     4681 atattcatac aaaactggtg aaaaaattcg tgttgcaact aaacaaggaa tcaaatcaac
     4741 tcgtgaagtg attgcgttga acgttgctgg tgctcttgat atctatgatg atgttgaagt
     4801 tggtcgtcaa gttttggttg acgatggtaa acttggtctt cgtgtggttg ctaaagatga
     4861 tgaaactcgt gaatttgaag ttgaagttga aaacgatggt atcatcgcta aacaaaaagg
     4921 tgtgaacatc cctaacacta aaattccttt cccagctctt gctgaacgcg ataacgacga
     4981 tatccgtttc ggtcttgaac aaggtatcaa cttcatcgca atttcattcg tacgtactgc
     5041 aaaagatgtg aacgaagttc gtgcaatctg tgaagaaact ggaaacggac atgttcaatt
     5101 gttcgctaaa atcgaaaacc aacaaggtat cgataactta gacgaaatca tcgaagcagc
     5161 tgatggtatt atgattgctc gtggtgatat gggtatcgaa gtaccgttcg aaatggttcc
     5221 agtttatcaa aaaatgatta tcaagaaagt caatgctgca ggtaaagttg ttatcactgc
     5281 aacaaacatg cttgaaacaa tgactgaaaa accacgtgca actcgttcag aagtatcaga
     5341 cgtattcaac gctgttatcg acggaactga cgctacaatg ctgtcaggcg agtctgcaaa
     5401 cggtaaatac ccactcgagt cagtaactac aatggctaca atcgacaaga acgctcaagc
     5461 tcttcttaat gaatacggac gtcttgattc agattcattt gagcgtaact ctaagacaga
     5521 agtaatggct tctgctgtta aagatgctac tagctcaatg gatatcaaat tggttgtaac
     5581 tcttactaag acaggtcata ctgcacgttt gatttctaaa taccgtccaa atgctgacat
     5641 cttagcattg acatttgacg aattgacaga acgtggcttg atgttgaact ggggtgttat
     5701 cccaatgttg acagatgctc catcttcaac tgacgatatg ttcgaaatcg ctgaacgtaa
     5761 agcggtagaa gcaggtctcg ttgagtcagg cgatgatatc gttatcgttg ctggtgtgcc
     5821 agtaggagaa gctgttcgca caaacacaat gcgtatccgc acagtacgtt aagaaaaata
     5881 taaaaaccta tcatatccag ctttctagcc tgtatgatgg gctctttttt tgtccagggg
     5941 gcaaaacttt cataatggat tgatactctt cgaaaatctc ttcaaaccac gtcagcgtcg
     6001 ccttaccgta ctcaagtaca gcttgcggct agcttcctag tttgctcttt gattttcatt
     6061 gagtatgaaa taagatatgc acaaattgat tagaaagtca aatgaatttc tacaaatgtt
     6121 ttagcaatcg taatgtactt gtctagattc gatctgatat attttcgatt taatgatatg
     6181 gtatttaaaa ccttcaaagt agcttgctcc attcttttac ttacgtgagt gtagatgtta
     6241 tttactgttt tagcgttttt gtgttccact ctaaccatta tagcattctt ctcagctagt
     6301 gtactaagga gtgtgtgcct gaaaatatgg gaactaaggg gctggtttat cggtttctct
     6361 agtttagtat ttgccttttg caaagtgatg ttaaatgcct ttctctaaat ttacatatca
     6421 ctattgttta acaaaatcta atctatttta ggtcacttat tctttttttg aaatgtagaa
     6481 tgaacttttt caaagttttt cgaatctttt aaaatctgtt tgttttatat cgccattctc
     6541 cctatatagc ctgacaactt tcccgatggt acgaatatgg ttgctttcgt ctaggtggat
     6601 gtcggggtat ttgggattga gtttttttaa ggtagccttg gcggagtttc ttgacatagt
     6661 tagcgccgtc tacttggaag atgccgatgg tattatagtc aatctgtggg gtattcttga
     6721 taaataggta gtcgctgttt cttatctttg cctccatgga cttgctgacg acataagcga
     6781 ttgggtcgta gtcgtctggg ataatggaaa ctccatatct aaatcgttgt cctgcatcga
     6841 gcggctacct gcagagataa actacctaac acgagagtaa gtagtctgtc tgtagtcgtc
     6901 cagtctgatg atttttacga tacttcgttt ttctgatcat acagttgcct ctcggcatag
     6961 gtcagaactt taccttgtct gggtggtgcc cgttggtcgt agatagattg gatatcgcta
     7021 ggagaatcct tttgaactgg agaaaagagg gcatcaatca agctactgaa tactttaact
     7081 aagtcaaata tagtattttt cttagtagac ctaacccttt tttcataatt tccaatggtg
     7141 tttttactta taccaatctt agtacccaat tcttattgag tccaaccatt actagtctat
     7201 attgttttat agttgattga gtttggaata gtacgctgta gctgctaaaa catttctagg
     7261 aattaatttg cctttcctaa tagagttgtt catatcttat ttcaatctac tatatttttc
     7321 gcctctaaca atcgcaatct cttctttatc tacgaatgaa atcgctttct atttttgtaa
     7381 gtaaagcatc acacgaaatc cacgaaaatg aaaacctttg ttgtgttttc gtaaaaaatt
     7441 tgttgacaga gcacgaaacg cctactataa ttaaatcaag cttaaggaaa taacaaaaaa
     7501 acaaatcgga gggaaagacg atgaacatat taaatgagaa agtaatcaat atctgtaaag
     7561 cagtagttaa agaaacttta atccaagaca tttaggagaa agaaatgaag aaatcaagaa
     7621 aattagccac attaggaatc tgttcagctt tgtttttagg cttggctgct tgtcaacaac
     7681 aacatgctac ttctgagggg acgaatcaaa ggcaaagcag ttcagcgaaa gttccatgga
     7741 aagcttcata caccaaccta aacaaccagg taagtacaga agaggtcaaa tctctcttat
     7801 cagctcactt ggatccaaat agtgttgatg cattttttaa tctcgttaat gactataata
     7861 ccattgtcgg ctcaactggc ttatcaggag atttcacttc ctttactcac accgaatacg
     7921 atgttgagaa aatcagtcat ctctggaatc aaaagaaggg cgattttgtt gggaccaact
     7981 gccgtatcaa tagttattgt cttttgaaaa attcagtcac cattccaaag cttgaaaaga
     8041 atgaccagtt gcttttccta gataatgatg cgattgataa aggaaaggtc tttgattcac
     8101 aagataagga agagtttgat attctatttt cgagagttcc aactgaggca actacagatg
     8161 tcaaggttca cgctgaaaag atggaaacat tcttctcaca atttcaattc aatgaaaaag
     8221 ctcgaatgct gtctgtagtc ttgcacgaca atctggatgg cgagtatctg tttgtaggcc
     8281 acgttggggt cttagtacct gctgatgacg gtttcttatt tgtagagaaa ttgactttcg
     8341 aagagcccta ccaagcgatt aaatttgcta gtaaggaaga ttgctacaag tatttggaca
     8401 ccaagtatgc ggattataca ggcgagggac tggctaagcc ttttatcatg gataatgata
     8461 agtgggttaa actttaatag tgttatgata tgacagtaat cttaggattt ttatttctat
     8521 ttttgatgag ttggtgggtt atttcaatcg ttaatagcca gaaaccacgc aaaaatgaaa
     8581 catttattgg ctatgtccaa agatacgata ttgatgggaa tggctatcaa tcattaccgt
     8641 gtcctcagaa ctgagaggag ttcttgaaat cgtaaaacca ctttgaacaa gagttacttc
     8701 aacccattgg ctccgacgga ttaagttgct ttcgtgaata ccaaaatcag ccgcaatttc
     8761 ttcataagtg cggtattctc gcacatattg aagagtggcc ataagaaggt cttctaggct
     8821 taatttaggt tttcgtccac cttttgcgtg tttaagttga taagctgttt ttaatacagc
     8881 taacatctct tcaaaagtcg tgcgctgaac accaacaaga cgcttaaatc gtgcatcagt
     8941 tagttgttta cttgcttcat aattcataga actattatac catgttttgt ttcgcaggaa
     9001 atctaatgct aaaatcttat cagtgagaag atttcttaaa gtttattctt tgtagtttca
     9061 tctgtattag tacataattt aacttagaga aaataggagg actaatcgaa tggaattaaa
     9121 agattttaca gaaaaagaac aggaaatgat taagaaaagg cttacaatgt ctaatattag
     9181 tgacaaagaa actactgaga agattctggc gctagtacca caagacttga ttaagcgaat
     9241 cccgtttttt gtcagaaaac atgctacaac acgtacgatt aaacgcattt caattgaaca
     9301 ccctgaactc tacgctgtag ctcaaacaag tggtgagatt ccagaaaaag aacgcgaaga
     9361 attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata gtattaagta
     9421 gagaatgaaa aaatatttta ttggcggttt gggaaacaat gcctatcata gcaaggattt
     9481 tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc atcttcgaga
     9541 tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat ctatctgtct
     9601 gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat ttgaagaagt
     9661 aaagaaactg attcttttgg atggtggcta tctagattta gataagattt tacctttgga
     9721 tacagagtta gaggaaacta aaaattatat caaatctcaa attatttcgg acttagatgt
     9781 tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg agaaagctgt
     9841 aagacagtcc tatcactgga atgttgagta taatagatat gagttggcta taaattatga
     9901 aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga gagaagtggg
     9961 agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag aggaagccct
    10021 aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc atgagcttta
    10081 tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt attttctatg
    10141 agctacttgt attgctggaa atggtataag atgaaaatct acaagtcact aattttcata
    10201 taaccaaaaa acgaacaggc tctttttctg ttattcagaa gagaacttgt tcgtttttat
    10261 cttgattttc ttaaaaagct ctatatacat agggattttc tggtttatcg actttggtaa
    10321 agctgtcgat ttccaaggtt ggaaggtttt gtttgagttc tttatggtag tgattgacca
    10381 gttttccttt ggctgaaatc caagtgttgt tttcatactg ccgggtattg cccttggtca
    10441 gcaatccata gacaccagaa tctgcgatgc agtgaataat gccgaagcgg aacagaaatt
    10501 ggctattggc atgactgggg tcattataga caaagcctgt gaactggatt gtcttaccct
    10561 caaattcatc tggatagtcg tagatagcct ccatgacctc catatagttt tcattagtga
    10621 tctgaatact atcttgggat aagtatttat ccgccgccgt tcgcatttcc ttttcatagg
    10681 ctgattttga aaaataagaa ctggtatctg gtttcaaata ttggcttgtc gtcccttcgc
    10741 ttgtctgaat ggctagatcc gttccttccg atagggggaa atgataacct ttagcagaaa
    10801 cggtctgaga atccaagcta acagttggga aagttaagcc gatgacaagt ggaatagcca
    10861 gaagagaaat actcgtcatc ttggctaatc ggctgttcag atgactgtgg gttttgactt
    10921 gcttcatcca gatatacaat tgaacgatag ccaagataaa agaaagcacc atggagatat
    10981 aggccagata ggaatagtgc atgttgatgt actggtttag tttgcccgac agatggaggt
    11041 aaatagtcag ttcaaaatag ccagctaaaa ctaaaaatcg aatcatagga tgactccaat
    11101 caagagagaa tagactaaga cgacaagagt tacaattgtt atgaagtgac tgataaatcg
    11161 tgctttcaag taatttttca tcatgagaat atttttgata tccagcattg gaccaatgac
    11221 gagaaaggcc agaactggtg ccaaaccgaa actcgagaga agagaagcac ctataaaggc
    11281 atccgcctca ctacagagcg aaagaagaaa ggctaaaatc atcaagagca ggatggcaaa
    11341 aagaggggtc gcactgatag aggtcagaat ccgagtcgga acgtagacct gtattataga
    11401 agcaaagagg cagccaaata ccaaataacg ccccgtatca aaaaattcat caatggcctg
    11461 cacaaagact tgaaaaactt tttttgcaga actcaagtaa gaaaaatcat gctcatgaca
    11521 agccagacga ttttctttct gaatcggttc ttgccagaaa aatcctagaa aaattcctag
    11581 tattacagcc acaagaatgg aacccagagc tcgtaatagg gcgacatgga aggagttgcc
    11641 aaaggcagaa taggtcgcaa aaagaacaat gggattgata acaggtgctg tcacaagaaa
    11701 aggaacggcc gtgtaacttg gaaccttttt ttccagaaaa cgattgatga tggggacgat
    11761 tccacattca caagaaggga aaaacatacc gacgaaggtc ccaaaaaaga ttctccccca
    11821 acgatttcga gggagaaaat gataaacctt gtcaggtgtg atataaactt caatcagccc
    11881 tgagacaatg cttcctatca gaacgaaggg aagggcttca atgataatgg agagaaaaat
    11941 ggctccagtt tgaagtacac tagatgggag ttgttgaaaa gcagtcatct agtttttctt
    12001 ttctgctttg gcttttttct ctttgtcatc agggaaagta acttgtttca aatcagggag
    12061 tttagcaaac tcttcaaaca tcttgtccaa gtcgtctcgt tttgtaaatt ttacagccat
    12121 aggtgtacct cgattctaaa ttctacctct attatactat tattttgcgg aaaagggagt
    12181 tttttaattt gttttcgata gttgatactc ttcgaaaatc tcttcaaacc acgtcagctt
    12241 cgccttaccg tagtacggtt actgacttcg tcagttctat ccacaacctc aaaacagtat
    12301 tttgagcagc ctacggctag cttcctagtt tgctttttga tttttattga gtatgaaaag
    12361 gattggagaa agccttccag cttgtggtat aatagattta tggataaaaa atatgaaaaa
    12421 atctctcagg atttgggagt gacgttaaag caaattgata ccgttctaag tttgacagct
    12481 gaaggggcga ctattccctt tatcgcgcgt tatcgcaagg acatgactgg tagtctggat
    12541 gaggtggcga ttaaggctat tattgatttg gataaaagtc tgacaaatct caatgaccgt
    12601 aaggaagctg tcttagctaa gattcaagaa caaggtaagt tgaccaagga attggaagaa
    12661 gctatcttag ttgccgaaaa attagcagac gttgaagaac tctatcttcc ttataaggaa
    12721 aagcgtcgta ccaaggcaac cattgcccgt gaagctggac tctttcctct tgctcgcttg
    12781 attttgcaga atatagttga cttagagaaa gaagctgaaa agttcgtctg tgaaggattt
    12841 gcgactggca aggaagcctt gaccggtgca gttgatattt tggtcgaagc cttatcggaa
    12901 gatgtgacct tgcgttctat gacttatcag gaagtgctga gacactctaa actcacttct
    12961 caagccaagg atgaaagtct tgatgaaaag caggtttttc agatttatta tgatttttca
    13021 gagacagttg gaactatgca aggctatcgt accttggctc tcaatcgtgg ggagaaactt
    13081 ggtgtcttga agatcggttt tgaacatgcg acggaccgta ttcttgcctt ctttgctact
    13141 cgtttcaagg tgaaaaatgc ttatattgat gaagttgttc agcaatccgt taagaaaaag
    13201 gtcttgcctg ctattgagcg tcgtattcgg acagaattaa ctgagaaagc tgaagaggga
    13261 gctatccaac ttttttctga caatctgcgc aatctcctct tggttgctcc actgaaaggg
    13321 cgcgtggttc ttgggtttga cccagccttt cgtacaggtg ccaagctagc tgtcgtggat
    13381 gcaacaggaa aaatgctgac aactcaggtt atttatcctg ttaaaccagc atcagctcgt
    13441 caaatcgaag aagccaagaa agatttagca gatttaattg gtcaatacgg tgtagagatt
    13501 attgccattg gaaatggaac ggccagtcgt gaaagtgaag cttttgtagc ggaagttctg
    13561 aaagatttcc ctgaagtcag ctatgttatc gttaatgaaa gtggtgcttc tgtctattct
    13621 gccagcgaac ttgctcgtca ggagtttcca gacttgaccg ttgaaaaacg atctgccatt
    13681 tctatcgccc gtcgtttgca agatcctctt gcggaattgg tcaaaatcga tcctaagtca
    13741 attggtgtcg gtcaatacca acacgatgtc agtcagaaga aactatctga gagtctggac
    13801 tttgttgtcg atacagtggt taaccaagtt ggtgtcaatg tcaatacagc tagcccagct
    13861 cttctttcac acgtagctgg actcaataaa accatttctg aaaatatcgt caaataccgt
    13921 gaggaagagg gaaaaatcac ttcacgctcc caaatcaaga aagttcctcg tttgggggcc
    13981 aaggcttttg aacaggctgc tggtttcctc cgtatccctg aaagtagcaa tatccttgat
    14041 aatacaggag ttcacccaga aaattacgct gccgttaagg aactcttcaa atgcttggac
    14101 attaaggatc tgaatgaaga ggcccaaagt aaactcaagt cgctttcagt caaggaaatg
    14161 gcgcaagagt tagaccttgg tccagaaact cttaaagata tcattgcaga tcttctcaaa
    14221 ccaggtcgag atttccgtga ttcctttgat gctccagttc tccgtcaaga tgtcctagat
    14281 atcaaggact tagtggttgg acaaaagcta gaaggagtgg tgcgtaatgt tgttgatttc
    14341 ggtgcttttg ttgatatcgg aattcatgag gacggtctca tccatatttc ccatatgagt
    14401 cgcaagttta tcaaacaccc tagtcaagtg gtggcagttg gagatttggt gagcgtttgg
    14461 gttaagaaaa ttgatactga acgtgaaaaa gttaatctgt ctctcctagc tccaaatgaa
    14521 actgactgat tacgtcaagc aggtttcact agaagacttc ggcagacctt ttatccatca
    14581 tgtccagtgg aataggcgtc tacgttcgac aggtgggcga tttttcccca aagatgggca
    14641 tttggatttt aatcccaagg ttggggttgg acgtttttag gaaaattgtc cgacatgaac
    14701 tctgtcatta tcacctctat tttcaaggga aaggctatca acacaaggat cgggatttta
    14761 aggaactgtt gaaagcagtg gatggattac ggtttgtacc atccttgccc aatagtaact
    14821 ccaagccaat caagctctat cgttgccaat cctgccagca aacttatcag cgtaagcgta
    14881 ggattgatac ccaacgctat cgctgtggac tttgtcgagg aaagttgcta ttagtaaatc
    14941 agcctgagga ctgatgaaag ccgagcctat ccgtgatata ctagtctaac ctagcagaaa
    15001 gaggaaatat catgaacagt aagttttata aaatgagacg aaatcgcatg atttcaggag
    15061 ttttagcagg tctgtcagac aagtggaact ttgatgttac ccttgtacgt tttctgtttg
    15121 ccatttttac agtcgcaaac tttggacttg gggtgattat ctatatcatc ctagcttcta
    15181 tcatgccaac caaggaagaa atcgaagcag aaatgtacgg aacaggccca cgtaaacgca
    15241 aagaagctca agccattgac gataatgaag gctggttttg gtgaggtttt acaaaagatt
    15301 tggaatacgc aaaaagcttt aaatcaatag tctaaactgc ttgatttaaa gcttttttac
    15361 ttttatactc aataaaaatc aaagtgcaaa ctaggaagcc agccgcaggc tgtaatttag
    15421 tcggcaatgt gaagttgata tagtttgcac tttgattttt gaatagtatt aataatattt
    15481 cgccagcttc atccacaaac tatagtggat tggaactaga ataggacacc tatacttcta
    15541 aaaaattgtt agaaatcgat ttgactgtcc tgatcgattt gtcctgttct tatttcattt
    15601 tactataaaa gcagtaattt aaacgcagtt ttttaatcta tttttgctgt tgagacaata
    15661 catttttgga aaaatggtgg atgctgataa caactgataa ccagcaaata acataagcag
    15721 tgccggtaaa ttctgtaccg ataaagtgtg tgtgttcaac taagagctga ccgatgaatg
    15781 gagagagcag atagagaagt ccgatgagta tatgttcttt gtgtttcatt aaagcgcacc
    15841 ttctttcctt ataggttgaa cgacttgcaa gtgttaaatt gaatggtatg ttatctaggt
    15901 aagcttactc aaatgatagt tcccgcttcc tccttttcaa agtaagcctt ttcacactta
    15961 tattttacaa ctttaaaaat aggaaatcaa atcattttct aagtaatttt gcttatattt
    16021 cacccaatct tacaaaaatg taagattaaa gtctcttttg taaggttagg ttatggcaag
    16081 cggtcttttt ttgatgtaaa ctagactcat aaagaacaaa ggagtaacat catgaaacaa
    16141 atcttacata agaaaactag aaaaccaagt cacaaagaaa ttgagcgcgt tcagctggga
    16201 tgcactatga tgcaagctac atttcatttg atgggatatt aagaaaggag attctcatga
    16261 cacttttaga tgtaaaacac gttcaaaaaa tttataaaac acgttttcag ggcaaccaag
    16321 tagaagccct caaggatatt cactttaccg tcgaaaaggg tgactacgtt gccatcatgg
    16381 gtgagtctgg ttctggtaaa tcaactcttc tcaatattct agctatgctg gataaaccaa
    16441 gtcgtggtca ggtttacttg aatggaactg acaccgcaac cattaaaaat tcacaggcct
    16501 ctagcttccg tcgtgaaaag ttgggctttg tcttccaaga ctttaacttg ctagatactc
    16561 tatctgtaaa ggacaatatc ttgcttccgc ttgtcttgtc aagaagacct attacagaga
    16621 tgatgaagaa attggtggtg acagctgaga atctgggaat aaaccaatta caagagaagt
    16681 acccttacga gatttctggt ggtcagaaac agcgtgtagc agtagcccgc gccatcatca
    16741 cagaacctga aattctcctt gcggacgaac cgacaggagc tcttgactcc aagtcatctg
    16801 cagccttact tgatgtcttt gatgaaatca atgagcgcgg ccaaaccatt ctcatggtaa
    16861 cccactcaac agcagcagct agcagagcta aacgtgtact ctttatcaaa gacggcattc
    16921 tttacaatca aatctaccgt ggagagaaga cagaacgtca gatgttccaa gaaatctctg
    16981 ataccttgac tgtcatggca agcgaggtga attagtatgt ttcgattaac caataagtta
    17041 gcggtatcga acttgattaa aaaccgcaaa ctctactatc cttttgcgct ggctgttctc
    17101 ttggcagtca ccatcaccta tctcttttac tccctaacct tcaatccaaa gattgcggaa
    17161 atccgtggag gaaccaccat tcaagcaaca cttggatttg gtatgtttgt cgttaccctt
    17221 gcgtcagcca ttatcgtcct ctatgccaat agttttgtca tgaaaaaccg ttccaaggaa
    17281 ctgggtatat atggcatgtt aggcttggag aagcgccatc taatcagtat gacctttaag
    17341 gagttagtgc tatttgggat tctaactgtt ggagcaggaa tcggtattgg agccttgttt
    17401 gacaagttga ttttcgcttt cctactcaaa ctgatgaaat tgaaggtgga actggtcgct
    17461 accttccaga tgaaagttgt cattacagta cttcttgtct tcggtttgat tttcctaggc
    17521 ctcatgttcc tgaatgccct tcgaatcgcc cgtatgaatg ccctccagct ctcacgtgag
    17581 aaagctagcg gagagaaaag aggtcgtttc ctacctctcc aaactattct tggttccata
    17641 agtttaggga ttggctatta tcttgccctt acggtaaccg atcctcttac agccctaaca
    17701 actttcttcc tagctgtttt gctggttatc tttggtactt atctattgtt taatgcaggg
    17761 attacagtct tcctacaaat cttaaagaaa aacaagaaat actattacca acctaataac
    17821 ctcatatctg tttccaactt gattttccgt atgaagaaaa atgcggttgg actagcaacc
    17881 atcgctattt tgtcaacaat ggttttggta accatgtcag cagcgacaag cattttcaat
    17941 tccgcagaaa gctttaaaaa agttctaaat cctcatgatt ttggggtttc agggcaaaat
    18001 gttgaaaaag aagatttgga caaactcttg agccagtttg caagtgacaa aggttatagt
    18061 gtcaaagaga aagaagtact tcgttacagt aactttggta ttgcaaatca agaaggaacc
    18121 aagttaacta tttttgaaaa aggacaaaac cgtgtccaac ccaaaacagt tttcatggta
    18181 tttgaccaaa aagattatga aaatatgact ggtcaaaaac tgtctctatc aggaaatgag
    18241 gtcggtctct ttgccaaaaa tgacggactg aaaggacaga aagctctaac tctaaatgat
    18301 catcaatttt ctgtcaaaga agaatttaat aaagatttca ttgtcaacca tgttccaaat
    18361 aagtttaata tcttgactac tgattacaat taccttgttg ttcctgattt acaagccttt
    18421 ttggatcaat tcccagattc ggctatctat aatcagtttt acggtggtat gaatgtagat
    18481 gccagcgaag aagaacaact caaggtcgct gaggagtatg aaaactacct caatcaattt
    18541 aatgctcaat tagacacaga aggtagctat gtttatggta gcaatctagc agatgctagt
    18601 tctcagatga gtgccttctt tggtggtgtc ttctttatcg gtattttcct atccattatc
    18661 tttatggtcg gaactgttct ggtcatctac tacaaacaaa tttctgaagg ctacgaagac
    18721 cgtgaacgct ttattatctt gcagaaagtc ggtttggacc aaaagcaaat caagcaaacc
    18781 atccacaaac aggttttaac tgttttcttc cttcctttgc tctttgcctt catacatctc
    18841 gcctttgcct accatatgct tagcctgatt ttaaaagtga ttggtgtact ggatacgact
    18901 atgatgttga ttgtgacctt gtctatctgc gctatcttcc tcatcgccta tgtgctgatt
    18961 ttcatgatta cttcaagaag ttatcgcaag attgtgcaaa tgtaaaaaag atacctcgac
    19021 ttcaaaatcg aggtatttct tgtattttaa atgctgaaaa gttgttcgag caggaaggta
    19081 actcccatgg tcaagagacc aatagcaagg ttccgaatca tagctgtttt ggttggggct
    19141 tttccaagtc tagcacttgt gtaaccagtg agaagaaggg ccacaccgac aataaggacg
    19201 gtagcaggga tgcggtaatc acttggaaaa atggtcactg agagcattgg aggcaaactt
    19261 ctaaggaaaa aggcaacgaa gctagaaatg gcagcgtgcc aaggattggt aaattcttca
    19321 tactcaatcc catatttttc ctctaccaga gccttgagtg gatttttaag aaagatctta
    19381 ttagtcaaga gttgggcaga agttttgaat tctccatttt ggatataaga agcatagagg
    19441 gattttttgg ctagttccct atcttggtct agcaagagtt tttctcgcga aacggcagct
    19501 tcctcggtat cttttggagt tgaaacggat acatattctc caccagccat tgaaaaggca
    19561 ccagctaaga tagccgcaaa acctgataaa aagataatcc agatattgct cgtggcactg
    19621 gcaactccga taaccacacc agcaatggaa ataattccat cgttagcatc aagaacaccc
    19681 gcacgcagga tatttaaacg acctgcaaaa tttgaatcaa tttcgtgatt tgtttctgac
    19741 gctaaatttc aagttcaagt tagccatcca gaagtcttct ctgagtgact tgtagtccaa
    19801 gcatttttta ggatagttgt t
//
LOCUS       DAVKJU010000038        18681 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVKJU010000038 DAVKJU010000000
VERSION     DAVKJU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18681
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.038"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(293..1099)
                     /gene="yidA"
                     /locus_tag="VW191_001292"
     CDS             complement(293..1099)
                     /gene="yidA"
                     /locus_tag="VW191_001292"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEU9624055.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYEHYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(1112..2416)
                     /locus_tag="VW191_001293"
     CDS             complement(1112..2416)
                     /locus_tag="VW191_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU9624056.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2488..2865
                     /locus_tag="VW191_001294"
     CDS             2488..2865
                     /locus_tag="VW191_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEU9624057.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTNRYQVDGQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2956..3288
                     /locus_tag="VW191_001295"
     CDS             2956..3288
                     /locus_tag="VW191_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEU9624058.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3300..4871
                     /gene="ffh"
                     /locus_tag="VW191_001296"
     CDS             3300..4871
                     /gene="ffh"
                     /locus_tag="VW191_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU9624059.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKVLAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(5066..6349)
                     /locus_tag="VW191_001297"
     CDS             complement(5066..6349)
                     /locus_tag="VW191_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEU9624060.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMLATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6603..7316)
                     /gene="rsmG"
                     /locus_tag="VW191_001298"
     CDS             complement(6603..7316)
                     /gene="rsmG"
                     /locus_tag="VW191_001298"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU9624061.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7410..7970
                     /locus_tag="VW191_001299"
     CDS             7410..7970
                     /locus_tag="VW191_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU9624062.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7972..8871
                     /gene="htpX"
                     /locus_tag="VW191_001300"
     CDS             7972..8871
                     /gene="htpX"
                     /locus_tag="VW191_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEU9624063.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8915..10474)
                     /locus_tag="VW191_001301"
     CDS             complement(8915..10474)
                     /locus_tag="VW191_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9624064.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLSKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIHSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10713..10838)
                     /locus_tag="VW191_001302"
     CDS             complement(10713..10838)
                     /locus_tag="VW191_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9624065.1"
                     /translation="MIELGSLKLNNQERTAWRNYDKFHYEHVKHVLSLYGPVFGF"
     gene            complement(10889..11029)
                     /locus_tag="VW191_001303"
     CDS             complement(10889..11029)
                     /locus_tag="VW191_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blpT protein"
                     /protein_id="HEU9624066.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKQ
                     KS"
     gene            11199..11741
                     /locus_tag="VW191_001304"
     CDS             11199..11741
                     /locus_tag="VW191_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU9624067.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            11741..12370
                     /gene="nth"
                     /locus_tag="VW191_001305"
     CDS             11741..12370
                     /gene="nth"
                     /locus_tag="VW191_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU9624068.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            12581..13102
                     /gene="pyrR"
                     /locus_tag="VW191_001306"
     CDS             12581..13102
                     /gene="pyrR"
                     /locus_tag="VW191_001306"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU9624069.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            13121..14044
                     /locus_tag="VW191_001307"
     CDS             13121..14044
                     /locus_tag="VW191_001307"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU9624070.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            14094..15173
                     /gene="carA"
                     /locus_tag="VW191_001308"
     CDS             14094..15173
                     /gene="carA"
                     /locus_tag="VW191_001308"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU9624071.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            15486..18662
                     /gene="carB"
                     /locus_tag="VW191_001309"
     CDS             15486..18662
                     /gene="carB"
                     /locus_tag="VW191_001309"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU9624072.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         5426 a         3596 c         4200 g         5459 t
ORIGIN      
        1 tatttttagt gaagtatcct aatttgtata attgattgtg tgtagattca gggtcaattt
       61 gaagagagta tttaaaatat cttgggaaat tataatttct ccctgttttt tgagtccaat
      121 ctatgagtat gagttgccca ggagtaaggc cacattctaa atcgtttagg taaaaattgt
      181 tacaatcaga atttgtgtct ctattatgat tattttgttt taaattaaat agtttgctaa
      241 aaaaattcat tggattttat tgctaatcaa ttcttattga aaaatgatac ttttacagta
      301 cccatgttcg gatggcgtgg gcaacgccgg attcgtcatt tgttttagtg atgtatttgg
      361 cgattttttt gatttctgga tttccatttt ccatgacaac ggggtttcca acgacttcca
      421 gcatggcacg gtcattttct tcgtcaccga ttgccatggt ttcatctttg gtcaatccga
      481 gtttttcagc caagtgagta atagctgaac ccttgtctac attcttttta aggagttcga
      541 ggtagaaagg agcagatttg ttgatagagt agtgctcgta aaattcggct ggtatttttt
      601 caatcgcagc atcgagaatt tctggttcat cgatgaacat acacttgaca atttccttgc
      661 cagccatttc ttcaggggta cggtagaaga taggcatgct gacgagggtt gattcgtgta
      721 cagtgtattt tccgatattg cgatttgcag tatagatacc gtccttggta atggcatgca
      781 tgtggacacc gagcttgcga ctgaggaatt ccatatccag ataatcctca taggtcaagg
      841 attcgctgat aatctcatgg ccagtagcag tttcttggac aagggcacca ttgaaggtca
      901 ccacatagtc accttggtct ctcaactgca agtcgtccag aagtttggca acacctgcga
      961 tggggcggcc agttgcaatc acgactttga caccagcttc tttggcatct tggatggcag
     1021 aaaagacttc aggagtgatt tccttttggc tgttgactag ggttccgtca atatcgacgg
     1081 cgattagttt aatactcata aatttcctct tttagttttt atttggggta aaatgatcgt
     1141 tctcaatcaa gtgtaaaaat tgctgggtga tgcttgcgaa gatgctgttt tggtctaaca
     1201 tttctttcgg gaaataaaag cgattatctc cgtggcgact tccagcaagg gattggacga
     1261 taggggacag gctagagagt tctgccagtt ctccattttt ttgtaaaatc tcaatctgtg
     1321 tccttggatt ttcagattcg ggacggtaaa tatcataagg gaggtcaaag ttcttatgaa
     1381 tggcagtata gtagtcggga tcaaagccga tgtcctcaac caattttctc atgctagcga
     1441 gttggtcttg gtcttcttgt gaaaaggtaa tggatttaaa gaccttgcgg ttgacaaagc
     1501 gatgcgataa atctgcaaga atcttgtcag gactggtcat ccaaagctgg aagtaggtat
     1561 tcatcacgcc atcatccaga gccagatagt cagtcaaggt cacatttttt tcgaagaaag
     1621 gcaggaggtg tggagaagtt cgggcaaaga aatccttgtc ctcaggatag agttccttgg
     1681 cgcgtttgag aagattctgt aggagaactt ccatggcgcg tgttgcgggg tggaaataaa
     1741 cctgcatgta catctggtag cgactgagga cgtagtcttc gatggcgtgc atgccattgc
     1801 gctgaaaggc gataccattt tcgataggac gaatgactcg gaggattcga gtcaggtcaa
     1861 attccccata ggatgctcct gtaaaatagg agtcgcgcaa gagatagtcc atgcgatctg
     1921 cgtcaatctg actagaaatg agctgcacga cctgcttatt aggataggta tggtcaatga
     1981 cactggccac cttttctggg aaatcaggtg ccacttgtag caggacttgg tgaatctctg
     2041 tctcaggatt ttgaataatc tcctgagtaa tggcttcatg gtctgtatca aagagatttt
     2101 caaaagtatg ggagtaggca ccatgcccaa ggtcgtgtag gagagcagcg gtcatggtca
     2161 agagagactc ggcaggattc cattcctcag gatatttttc ttcgaaaatc tctgtgatgc
     2221 gtcgtgcaat ttcatagact cctagacagt gagagaagcg actgtgttct ccaccgtgga
     2281 aggtataact ggaagttccc agttgtttga tccggcgcaa acgctgaaat tcttttgtat
     2341 taatcaagtc atagatgatt tgattattga catggatgta gttgtgaaca gggtcacgga
     2401 atactttttc gttcatatga ccattatagc aaaatcctgc tctttttggt aaaatagtag
     2461 gacaagagac aagaaagagg gcctgatgtg aagattcgga tgcgaaatac aattcagttt
     2521 gatgagcagt tggaagtgat tgaccagctt tatgatgtgg aagtgcatga aaaaggagat
     2581 tatagctacc tgcttttcta taatgaggaa aaggaaaaag tagtgattaa atttcatggt
     2641 caagaacttg tgatgagtcg attttccaat cccaagacca ttatgcgctt tctaaaggat
     2701 agtgatagtt tagcctatat tcccacacct atgggtatgc aggagtttat tatccaaacg
     2761 aatcgttatc aagtggatgg gcaaaagatt gaactagatt atcaactaca aaatcaagag
     2821 ggacatccct ttgccagcta tcaattggaa attacttggg gctaggctca tgtctttttc
     2881 aaatttagct atctatcttc ttggatcggc ttcctagtgt ggtagattag gctaattttt
     2941 ggtataataa aagttatgga aatcgaaaaa accaatcgta tgaatgcgct ctttgaattt
     3001 tatgcggcgc ttttgacaga taagcaaatg aattatatcg agctctacta cgctgatgat
     3061 tacagccttg ctgaaattgc cgaggagttc ggtgtcagtc gtcaggctgt ctatgacaat
     3121 atcaagcgaa cagaaaagat tctggaagat tatgagatga aattgcacat gtactcggac
     3181 tatattgtcc gcagtcagat ttttgatcag attttggagc gctatcccaa ggatgacttt
     3241 ctgcaggagc agatagaaat tttaacaagc attgataata gagaataaga ggaagaaaaa
     3301 tggcatttga aagtttaaca gaacgtttgc agaacgtctt taaaaatcta cgtaaaaaag
     3361 gaaaaatctc tgaatctgat gtccaagagg caaccaaaga aattcgcttg gccttgctcg
     3421 aggccgacgt tgccttgcct gttgtaaagg actttatcaa gaaagttcgt gagcgtgcag
     3481 tcgggcatga ggtcattgat acgcttaatc ctgcgcaaca gattattaaa atcgttgatg
     3541 aggaactgac agccgtttta ggttctgata cggcagaaat tatcaagtca cctaagattc
     3601 caaccatcat catgatggtt ggtttacaag gggctggtaa aacaaccttt gctggtaaat
     3661 tggccaacaa actcaagaaa gaagaaaatg ctcgtccttt gatgattgcg gcggatattt
     3721 atcgtccagc tgccattgac cagcttaaga ccttgggaca acagattgat gtgcctgtct
     3781 ttgcacttgg aacagaagta ccagctgttg agattgtacg tcaaggtttg gagcaagccc
     3841 aaactaatca taacgactat gtcttgattg atactgcggg tcgtttgcag attgatgagc
     3901 tcctcatgaa tgagcttcgt gatgtgaaag tattggctca accaaatgaa atcttgcttg
     3961 tcgttgatgc tatgattggt caggaagcag ccaatgttgc gcgtgagttt aatgctcagt
     4021 tggaagtgac tggggtcatc cttaccaaga ttgatggtga tactcgtggt ggtgctgctc
     4081 tgtctgttcg tcacatcact ggaaaaccaa tcaagttcac tggtacaggt gaaaaaatta
     4141 cagatatcga aaccttccac ccagaccgta tgtctagccg tatccttggc atgggggata
     4201 tgctcacttt gattgagaaa gcttctcagg aatacgatga acaaaaagcc cttgaaatgg
     4261 ctgagaagat gcgcgaaaac acctttgatt ttaatgattt catcgatcaa ttagatcagg
     4321 tgcaaaatat ggggccgatg gaagacttgc tcaagatgat tccaggtatg gccaacaatc
     4381 cagcccttca aaacatgaag gtggatgaac gccagattgc tcgtaaacgt gccattgtgt
     4441 cttcgatgac acctgaagag cgtgaaaacc cagatttgtt aaatccaagc cgtcgccgtc
     4501 gtattgctgc tggttctgga aatacattcg tcgaagtcaa taaattcatc aaggacttta
     4561 accaggctaa acagctcatg cagggtgtta tgtctgggga tatgaataaa atgatgaagc
     4621 aaatggggat taatccaaat aaccttccta aaaatatgcc aaatatggga ggaatggata
     4681 tgtctgccct tgaaggaatg atgggacaag gcggtatgcc tgacttatca gctctcggag
     4741 gagcaggaat gccagatatg agccagatgt ttggtggcgg tttgaaaggt aaaattggtg
     4801 aatttgccat gaaacagtcc atgaaacgta tggctaacaa aatgaagaaa gcgaagaaga
     4861 aacgcaagta agacaagaga ttgatgcaaa tctcatttga tgagaaaata aagtaaaaat
     4921 ttctatgata aaacgtacag tatcaaggtt ttagaatatc tgatattata cgttttttga
     4981 ttttaataat tttgttaaga cacttttatt atggtatacg aaaaatccac tcagctgtct
     5041 gagtggatta ggtgtattta gactcttagt ctttattttc gtatggcaag atcaagttca
     5101 agatgattcc tgtcatggct gaaagggcag tacctgaaag tgtaactgga ccaagtttaa
     5161 ggatagctcc tccaagtcca agaaccaaca tagcacttgc gatgatgagg tttcgcattt
     5221 gagcgaaatc aacacgttct ttaatcaaga ctttcaaacc attgctggcg ataaccccat
     5281 agagaaggat tgacatacca ccaagtacag cgtttggaat agttgaaatc aaggcagtga
     5341 atttaccaag gaagctgagg gcaatcgcga tgaaggcagc gttacggata actgagacag
     5401 aagcgatacg agtcataccg ataacccctg tattttctcc gtaagttgta ttggctggtc
     5461 caccaaggaa ggcagaaaca gaagttgcga taccgtcacc aagaagagta cggtgaagac
     5521 ctggttcttt taagaattga cgaccacaga tttgacccaa aacagtatgg tctccgatat
     5581 gttcagaaat tgttacgata gcgattggca agatagcgat ggcttctgga ccaaagtaaa
     5641 gattgtactc tttaaaggca ccacctgtgc taaatggcaa gtagaaacca ggaatttcaa
     5701 accagttagc ttctaggaca ggcgtaaagt caaccaaacc aagagttagt gcgaagaggt
     5761 aaccaccgat aatggcaaag aggaatggaa tgattcgtag gaagcctttt ccttttgtat
     5821 tgataaaggc agcaattagg aaagtaacaa cggctaccag agcatttttc caatttccgt
     5881 ctgctacaag acctgcattg gtaacagctg aacctgcaag tccaagaccg ataacgatga
     5941 tcataggacc gataatgatt ggtggcaaga gtttatcaat ccattttgtt cctacaaatc
     6001 ggatgctggt agcaacaagg acatagacca aaccagtcaa gataacccct gtttgagcag
     6061 cagatacatc cccccccatt tctttcatag ccagtgacat agctgtgata aaggcgaatg
     6121 aagaacctag ataaactgga actttaaaac cagtagcaag catgtagatg agtgttccaa
     6181 cacctgaagc aaaaagggca acagatacag gcattcccaa aatcaatggt accaagatgg
     6241 tcgcaccaaa catggcgaaa acgtgttgga aactaaggag aattcctttt ccagccgaag
     6301 gacgttggtc aacgtctagt aacaaatcaa cagttgattc ttgtttcata taaacctcac
     6361 ttttgggcat caaaaaagag cccattattt tacagcaata ggccctcaga gtaagacttc
     6421 tttaaaattt agattttaaa gagttttaaa tatttctgcg tcttcgtcac ctcacgggat
     6481 gacattaaaa acctttgctt aggatagtat agcagaaaaa aatgattttg taaatcattt
     6541 tttcccgagc ctagaaataa aagagcgagg ctgattttgt aaacatttgt ttactaaaaa
     6601 atttaaagtg ggcgtttatt tggcatacca gccttacgtg gatatttatt tggtgtttct
     6661 tttttctttt ctaccactgt gatatagcgc ggatctctat tcggtagggc gtagctgaga
     6721 ttgtcttcga ccttactaaa aaggagattg agggcattct tagcttctaa taattcctca
     6781 ggcgcattgc tggccttgag tgccaatagt tttccaccaa ccttaaggta gggaatggtc
     6841 aattcagata ggacctgcat acgggcaacc gcacgagctg ttacaaaatc atattgagca
     6901 cggaagttct tgtcttgggc aaaatcttcg gcacgtccgt ggtagaaatg aactccgttc
     6961 aaatccagtt cttgagccaa gagttgtagg aagttgatgc gcttattgag tgaatcaata
     7021 atggtcacat ctaactccgg atagagaatt ttcattggta gactaggaaa tcctgcccca
     7081 gccccgatat caagaagttt gatagtttca ttgggaatca aaccttgaag aatgggtgca
     7141 atcgaatcgt aaaaatgttt gagataaact tcttccttgt ccgtaatcgc cgtcaaatta
     7201 atcttctcat tccactcgac caagagctca aaataacgtt caaattgttc tttttgctgg
     7261 tccgaaagtg gaagattctg ctcggcaagc aagttgtaaa atgtttttgg tttcatagtt
     7321 atttccttct ttagcatcat cttattttac cacaatcata gaaaatttga tatactggta
     7381 ctaatctaaa agcagaaagg acttatatta tgacttggat tattcttgga gttatcgctc
     7441 ttattgttat ttttgtgatt gttagctata acggtttggt taaaaatcgt atgcaaacca
     7501 aggaggcttg gagtcagatt gatgttcagt tgaaacgtcg aaatgacctc ttgccgaact
     7561 tgattgagac tgtaaaaggt tatgccaaat atgaaggttc tacccttgaa aaggtggcag
     7621 aactacgtaa ccaagtggcg gcagcgactt caccagcaga agctatgaaa gccagtgatg
     7681 ccctcactcg tcaggtttca ggtatttttg cagttgcaga aagctatcca gatttgaaag
     7741 ctagtgctaa ctttgttaaa ttgcaagagg agttgacaaa cacagaaaat aaaatttctt
     7801 actctcgtca actctataac agtgttgtca gcaactacaa tgtaaaatta gaaactttcc
     7861 cgagcaatat tatcgctgga atgtttggat ttaaagcggc agatttcctt caaacacctg
     7921 aagaggaaaa gtcggttcct aaagttgatt ttagcggttt aggtgactaa gatgttgttt
     7981 gatcaaattg caagcaataa acgaaaaacc tggattttgt tgctggtatt tttcctactc
     8041 ttagctcttg ttggttatgc ggttggttat ctctttataa gatctggact tggtggtttg
     8101 gttatcgcac tgattatcgg ctttatctac gctttgtcta tgatttttca atcgacagag
     8161 attgtcatgt ccatgaatgg agcgcgtgag gtggatgagc aaacggcacc agacctctac
     8221 catgtagtgg aagatatggc tctggtcgct cagattccta tgccccgtgt tttcatcatt
     8281 gatgatccag ccttaaatgc ctttgcgaca ggttctaatc ctcaaaatgc ggctgttgct
     8341 gcgacttcag gtctactagc tatcatgaat cgtgaagaac tagaagctgt tatgggacat
     8401 gaagtcagtc atattcgtaa ttatgatatc cgtatttcga ctattgcagt tgcccttgct
     8461 agtgctatca ccatgctttc tggtatggca ggtcgtatga tgtggtgggg tggagcaggt
     8521 cgcagacgaa gtgatgatga ccgagatgga aatggtcttg aaatcattat gctagtggtt
     8581 tccctactag ctattgtact ggcacctctc gctgcaacct tggttcagct cgctatttct
     8641 cgtcagaggg aatttctggc agatgcatct agtgttgagc tgactcgcaa tccccaggga
     8701 atgattaatg ccctagataa gttggacaat agcaaaccga tgagtcgcca cgtcgatgat
     8761 gctagcagtg ctctttatat caatgatccc aagaaaggtg gggggttcca aaaactcttt
     8821 tatacccacc cacctatctc agaacggatt gaacgtttaa aacagatgta aaagaatcca
     8881 gaggtcgtct ctggattttt gctatgttgc aaatttataa atcttctaaa ttaactagct
     8941 tcaaaccgtg ttctgtcatg cgatagatga tcgagcagac ttcttttaag aaacgacggt
     9001 catgcgaaac agtgatgaga ccgcctggat aggttgcaaa gagttttctg atttggggtt
     9061 gagaagtggg agaaaagttt cgtgtgggtt catccagcag gagaaagttt ggtttgcgca
     9121 ggactagatc caaaagcagg agttttccct gttgtccgcc agataaggag cgaatttgat
     9181 gctgcatttc tggataactg aaattgagac tggctaggtg agagtggatt ttctgaagtt
     9241 cctctttttc cccagttttg ctgatataag ctattggtga taaatccaat tgtagttttt
     9301 tgtggtaatc ttgtggcata aaaccaaggg aaatctctct tttatcattc agaagttgct
     9361 ttaatttggc taacagagtt gatttcccaa caccatttgg cccgataata ccgatttttt
     9421 cttgcccacg gacagttagt tgtagttttt gaaccaaaac tcggtcgtca atggacaaat
     9481 tttctttttc cagttggact aagactttag aagctggtaa tggttggatg tctgaaaaga
     9541 aaagttggat ttgttcctct tcaagtggtt tttgagtcat ggactgagct gccttttcgt
     9601 agcgtttttc ttgtgagagg acagttttca tctttttaga caataggcga ccggcagtac
     9661 tatctttggt agctcgaagc gcagtttcta cattttgctt aactctccga tgtttttcca
     9721 tggttttatc gtaggctctt tggttgttag cagcttgctg actttgtttg gcaaaattag
     9781 cctttctctg ctcactatag ctatcatagt ctaaatgctc tactagcgtt tccgcttctt
     9841 tacggtgttt gaccagtcgc aagtgaacaa tagtgtctgc cgtttcagaa agaaagtctt
     9901 catcatggga aatgaaaata acggtttgcc tggtcttttg aatctggcct tttagccaat
     9961 caactgtctc aaggtctagg tcatttgaag gttcatctaa aaatagaatc tcaaagggtt
    10021 tggctaactc atggataagc tgaattttca aagcttctcc ccctgataga ttgccaatct
    10081 cttggtcact tgcgaaacga ttgctatcaa aatgcaattc ctccgccaaa cgatagagga
    10141 tactgtagtc taaatcaata gaatctaaaa agaagtagtc gtgtaaagtt ttctttttca
    10201 gctcctcggg aagtttttga ggaatgtagg ccagtgactg atagtcagat tggatgtttc
    10261 ccttgatagt gaaatcagac aaagcttccc ccattaaaat tttaagtaag gttgatttgc
    10321 catttccttc ttcaccaata atagcaacct tttccccgtc ttggatggtc atgcttaggt
    10381 cagatacaag atctcgtaaa tctttgtttt gtgtgatggt tagatgattg atttttatca
    10441 tatgatttcc ctttctagaa tgttcatagt gcataacaaa aagctctact ttacctagta
    10501 gagcccaaag tctatgtcaa aaaaactcta ttaacatgcc agaaaaagag cataaaagag
    10561 cacacaaaaa gagacaaaaa caagatctac taaataaagg ttctttattt gatagactta
    10621 gttgttcatg tctcccttac ctccgagtat tagtatcttt atattatacc tttaaggaaa
    10681 ttttgtcaac agaaaactgg aaattctatg ctctaaaatc caaaaacagg tccatagaga
    10741 cttagtacgt gtttgacatg ctcgtaatgg aatttatcat agtttcgcca agcagtgcgt
    10801 tcttgattgt ttagttttag gctacccagt tcaatcagag gactggtggg ttagtaaaat
    10861 ttctcaagat caattaagat actgttgttc aacttttctg ttttttgagc tcttgcatag
    10921 tagtgtttag cagctcttgt agtcgaatat catctgccgt accctgttta cagttttggt
    10981 agcttttatt taacttggat aggagttgaa tgagtttctt gtcttccatg tgagcatcct
    11041 ccttgatctg atgtactctt tccctatagt atagcactaa aaaacaaaac tgtcatagta
    11101 aatatgttaa aatttcgaat tttaattaca aagtttggtg tgctgatttg tagccttttc
    11161 atggtataat caagagagta aatctttatg gaggaagtat gaaattaaat attcaagaaa
    11221 ttcgtaagca gtctgaaggt ttgaactttg aacaaacgtt agatttagtt gatgacctgc
    11281 gtgcacgtaa tcaagaaatt ttagatgtaa aagatatcct tgcagttggg aaagtacaat
    11341 atgaagaccg tatgtatttc ttagattatc aactatctta taccattgtt cttgcttcga
    11401 gtcgcagtat ggagccagtt gagttagttg aatcttatcc agtcacggaa gttttcatgg
    11461 aaggcgcaac taaccatcta aatcaagaaa ttttagatga tgacttggtc ttgcccatcg
    11521 aaaatgaggc gcttgacctt gctgagagtg tatcagacaa tatcttgcta aacattccta
    11581 tcaaggtctt gacggctgaa gaagaagctg gtcaaggatt tgtctcagga aatgactggc
    11641 aaatcatgac agaggaagaa taccaagctc aaaaagcagt aaagaaagaa gaaaacagtc
    11701 cttttgctgg cttacaagga ctatttgacg gagatgaata atggtcttgt caaaaaaacg
    11761 agcacgaaag gtgctagaag aaatcattgc cctcttccca gatgccaagc ctagtcttga
    11821 ttttaccaat cattttgaac tcctggttgc ggtcatgtta tcagcccaga cgacagatgc
    11881 agcggtaaat aaggccacac caggtctctt tgttgccttt ccaacacccc aagctatgtc
    11941 tgtagcgaca gagagtgaga ttgcttcaca tatttctcgc ttgggattgt atcggaataa
    12001 agctaaattc cttaaaaaat gtgcccaaca gttactagac gattttgatg gtcaagtccc
    12061 tcagacacgt gaagaattgg agagtttggc aggtgttggt cgcaagacag ccaatgttgt
    12121 catgagtgta ggatttggga ttccagcctt tgcagtggat actcatgtgg agcgtatttg
    12181 caaacaccac gatattgtca aaaaatcagc gacgccactt gaggtggaaa agcgggtcat
    12241 ggatatcttg ccgcctgagc agtggttagc tgcccatcag gccatgattt attttggaag
    12301 agccatttgc catccaaaaa atccagagtg tgaccagtac ccacaattat atgattttag
    12361 caatttgtaa gatgctacat tgaaactttt tgcttgattt taagcatgct ttgtgctata
    12421 gtaaatgata actaaatatt cctttaaacc tgtcccgtga ggcaggcaag gagcgcgata
    12481 aagtagaagg gtgaagtcta tactgtgtgc agtagggctc gcttggctat aaatttcaag
    12541 tctccttgtt taaggagact tttctttttg gaggtttgtc atgaaaacaa aagaagttgt
    12601 agacgaattg accgtcaaac gagcgattac gcgtattact tatgagatta tcgaacgcaa
    12661 caaagatttg aataagatcg tcttggctgg tattaaaact cgtggtgtct ttattgccca
    12721 ccgaatccaa gaacgtttga agcagttaga aaatctttca gttcctgttg tggaattgga
    12781 tactaaacct ttccgtgatg atgttaaaag tggagaagat acttctttgg tttctgtcga
    12841 tgtgacagac cgcgaagtta tcttggtgga tgatgtgctc tatacaggtc gtaccatccg
    12901 tgctgctatt gataatattg tcggtcatgg tcgtcctgcg cgtgtgagtt tagcagttct
    12961 agtcgatcgt ggacatagag aattgccaat ccgtccagat tacgttggaa aaaatatccc
    13021 aaccagtcgt tctgaagaaa tcatcgtaga gatgacagaa cttgatgacc aagacagagt
    13081 tctgattact gaagaagctt agaaagttaa aggagtagcc atgtcagaaa atcaacaagc
    13141 attgaaccat gtggtgtcca tggaagacct cactgtcgat caagtaatga aattgatcaa
    13201 gcgaggaatt gagtttaaaa atggagccca gcttccctat gaagaccatc cgattgtttc
    13261 caatctcttc tttgaggatt ctacacggac acataagtcc tttgaagtcg cagagattaa
    13321 acttggattg gaacgacttg actttgatgt gaagactagt tcggttaata agggtgagac
    13381 actttatgac accattttga ctctgtctgc tttaggagtg gatgtctgtg tgattcgcca
    13441 tccagaggtc gactactata gagaattgat tgctagtcca acgattacga cttccatcat
    13501 caatggtgga gatggttcgg gccaacaccc tagccagagc ttgcttgatt tgatgaccat
    13561 ttatgaggaa tttggccact ttgagggtct taaagttgct attgcaggtg acttggacca
    13621 ctcacgcgtt gccaaatcca atatgcagat tttgaaacgc ttgggatctg aactcttttt
    13681 cgctggacct gaggaatgga gaagtcaaga gtttgcagac tatggacagt ttgtaaccat
    13741 tgatgaaatc attgatcagg ttgatgtcat gatgtttctc cgtgtgcaac acgaacgcca
    13801 tgatagtgga gctgtatttt caaaagaaga ctaccatgcc caacatggct tgactcaaga
    13861 acgttacgat cgtttgaaag aaacagcaat cctcatgcac ccagctccaa tcaatcgtga
    13921 tgtagaaata gcagaccact tggttgaagc accaaaatca cggattgtcc aacaaatgac
    13981 caatggtgtc tttgttcgaa tggcaatctt agaatccgta ctagcgagta gaaacgccaa
    14041 ctaaaacaca agacgttaaa agacgccagt gagcgtccac gagaggtgaa aatatgacaa
    14101 aaagaattct agtattagaa gatggcacag tttttgaagg caaggccttc ggagcagata
    14161 ttgatgtaac aggcgaaatc gtctttaata cagggatgac cggctaccaa gaatccatta
    14221 cagaccagtc ttataatgga caaatcttga cctttactta tcctttggta ggaaattatg
    14281 gtattaatcg tgatgattat gaatccatta ttccaacttg taagggagtc gttgtttttg
    14341 aagaagcgcg tagagctagc aactggcgca accaaatgac cttggatgaa tttttgaaag
    14401 ccaagaaaat tccagggatt tcaggaattg atacgcgtgc tcttaccaag attatccgta
    14461 agcatggtac tatgcgtgca accttgaccc atgttgggga cagtatggac catgtgacgg
    14521 accagctcca agcaacagtt ttaccgacag acaatatcaa acaggtttct actaaaacgt
    14581 cttatccagc tccaggagtt ggtttgagcg tggtgctagt ggactttggt ctcaagcact
    14641 caatcctacg tgaactttct aaacgcaact gtaacgtgac agttgttcct tattcgacaa
    14701 cggctgaaga aattctccat ctcaatcctg acggagttat gttgtcaaat ggtccaggta
    14761 acccagaaga cgttccacaa gcactcgaca tgattcgcgg tgtacaagga aaaattccaa
    14821 tctttggtat ttgtatggga caccaactct ttgcaatggc aaacggggct aagacctata
    14881 agatgaagtt tggccaccgt ggattcaacc atgcggtacg tgaaatcgca acaggacgag
    14941 tagacttcac cagccagaac catggttatg cagtcagccg cgaggatttg ccagagcact
    15001 tgattattac ccacgaagaa atcaatgaca agtcagtgga aggtgtgcgc cacagatacc
    15061 aacctgcttt ctctgttcaa taccacccag atgcagctcc tgggccacac gacgctagct
    15121 acctatttga cgagtttatc gagatgatgg aagtttttaa acaatcaaac taagaacgag
    15181 taaaagctcg tcggagaatt tatgtaactg aacacggacg gaaagctcag aatgtagaga
    15241 aaccaacttg gattgtcaat ccttccttga tttcctaaaa ttcaatcgct ttctattcgt
    15301 cctttgtatc ttatttaatg tcgccctgag aggcgacgaa tagaaaggag aagggtgctc
    15361 cgtatttgct gaactgaata cgggctacgg acggtgctaa aaagatagtt attcctagaa
    15421 ctgacagttc ttcgtcataa ctcctatttt agctttgtcc gcttgacacc cttaatatct
    15481 tatatatgcc taaacgtact gatattcaaa aaattatggt gattggttct ggtccgatta
    15541 ttattggtca ggctgctgag tttgactacg ctgggactca ggcttgcttg tcgttgaaag
    15601 aggaaggtta tgaggttgtt ttggttaact caaatcctgc aaccatcatg acggacaagg
    15661 aaattgctga caaggtttac attgaaccga ttacacttga gtttgtgaca cgtattcttc
    15721 gtaaggaagg tccagatgcc ttgctaccaa cactcggtgg tcagacaggt ctcaacatgg
    15781 ccatggaatt atctaaaaat ggtatcctag acgagcttgg cgttgaactt ctgggtacca
    15841 aattatctgc cattgaccaa gcggaggacc gtgacctctt taaacaattg atggaagagc
    15901 ttgaacaacc cattccagaa tctgaaattg ttaacacagt agaagaagca gttgcctttg
    15961 cagcgacaat tggctaccca gtcatcgttc gtccagcctt tacccttggt ggtactggtg
    16021 gtggtatgtg tgccaacgag aaagaattgc gtgaaatcac tgaaaatggt ttgaaattgt
    16081 cacctgttac ccaatgtttg attgagcgtt cgattgccgg tttcaaggaa attgaatacg
    16141 aagtgatgcg tgactcagct gacaatgcct tggttgtttg taacatggaa aactttgacc
    16201 cagttgggat tcacacaggg gattccattg tatttgcccc tgcgcaaacc atgtcagact
    16261 atgaaaacca aatgcttcgt gatgcgagct tgagcattat tcgtgccctc aagattgaag
    16321 gtggatgtaa tgttcagcta gctcttgatc ctaacagctt caagtattat gttatcgaag
    16381 taaaccctcg tgtatcacgt tcgtcagccc ttgcttctaa agcgacagga tatccgattg
    16441 ctaagttggc tgccaagatt gcagtaggtt tgaccttgga tgaggttata aacccagtta
    16501 caggttcaac ctatgccatg tttgaaccag cccttgacta cgtagttgct aagattccac
    16561 gtttcccatt tgacaagttt gaaaagggtg aacgccgtct cggtacacaa atgaaggcga
    16621 ctggagaagt catggcaatc ggtcgtaaca tcgaggaatc tcttctcaag gcctgccgct
    16681 cccttgaaat tggggtgcac cacaatgaaa tacctgaact tgcagcagtt tctgatgatg
    16741 ccttgattga aaaggttgtc aaagcccaag atgaccgtct cttctacgta tcagaagcca
    16801 ttcgtcgtgg ctacacacct gaagaaattg cagagcttac aaaaattgat atcttctatc
    16861 ttgataaact cttgcatatc tttgaaattg agcaagaatt gggtgctcat ccacaagatc
    16921 tagaagtttt gaaaacagcc aaacttaatg gtttctcaga ccgtaagatt gctgaactct
    16981 ggggaacgac tgatgatcaa gttcgccaac ttcgcttgga aaacaagatt gttccagttt
    17041 ataagatggt agatacttgt gcggcagagt tcgactctga aacgccatat ttctattcaa
    17101 cctatggttg ggaaaatgaa tctatcagat ctgataaaga atctgtactt gtcctaggtt
    17161 caggtccaat ccgtatcggt caaggggttg agtttgacta cgcaaccgtt cactctgtta
    17221 aggctatcca ggcagctggt tatgaagcta tcatcatgaa ctcaaaccca gagaccgttt
    17281 caacagactt ctctgtatca gataagcttt actttgagcc attgacattc gaagatgtta
    17341 tgaacgtcat tgacttggaa caaccaaagg gagttatcgt tcagttcggt ggtcaaacag
    17401 ccatcaacct tgcggagcca ttggcaaaag caggtgtgac catccttggt acacaggttg
    17461 ctgacctaga tcgtgccgaa gaccgtgacc tcttcgagca agctctcaaa gaattggata
    17521 ttccacagcc accaggacaa acggctacca atgaagaaga agcagctctt gcagctcgta
    17581 agattggctt cccagttctt gtccgcccat cttatgtact tggtggacgt gccatggaaa
    17641 tcgttgaaaa cgaagaagac cttcgttctt acatgcgaac tgctgttaag gctagtccag
    17701 accacccagt tcttgtcgac tcttatatcg ttgggcaaga gtgcgaagtt gatgccattt
    17761 cagatggtaa aaatgtactc attcctggta tcatggagca tatcgaacgt gctggtgtcc
    17821 actcaggtga ctcaatggct gtttacccac cacaaacctt gtcgcaaaag gttcaggaga
    17881 ccatcgcaga ctacaccaaa cgcttagcaa tcggtcttca ctgccttgga atgatgaaca
    17941 tccagtttgt tatcaaggat gaaaaagtct acgttattga ggtcaatcca cgtgccagcc
    18001 gtactgttcc attcctatct aaggtaacca atattcctat ggctcaagta gcgaccaaac
    18061 tcattcttgg tcaaagttta tcagaacttg gctaccaaaa tggactttac cctgaaagca
    18121 cccgtgttca tatcaaggca cctgtcttct cctttaccaa actagctaag gtagacagct
    18181 tgctcggtcc tgaaatgaag tcaacaggtg aagttatggg ttctgatgct actttggaaa
    18241 aagctctcta taaagccttt gaagcttctt atctacactt gccaactttt ggtaatgtcg
    18301 tctttaccat cgctgatgat gccaaagaag aagccttgaa cttggctcgt cgtttccaaa
    18361 acattggtta tggaatcctt gcgacagaag ggacagcagc cttctttgct agtcatggct
    18421 tgcaagccca acctgttggt aagattggtg atgacgataa ggatatccca agttttgtac
    18481 gcaaaggaag aattcaagct atcattaaca cagtaggaac aaaacgaact gctgacgaag
    18541 atggtgagca aattcgtcgt tcagccattg aacacggggt gcccctcttc acagcgctag
    18601 atacagctaa tgccatgctt aaggtacttg aaagtcgtag ctttgttaca gaagcgattt
    18661 aaatagaaaa aataagatat a
//
LOCUS       DAVKJU010000039        18532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVKJU010000039 DAVKJU010000000
VERSION     DAVKJU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.039"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            953..2212
                     /locus_tag="VW191_001310"
     CDS             953..2212
                     /locus_tag="VW191_001310"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU9624073.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2228..2779
                     /locus_tag="VW191_001311"
     CDS             2228..2779
                     /locus_tag="VW191_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624074.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFSE"
     gene            2772..4049
                     /locus_tag="VW191_001312"
     CDS             2772..4049
                     /locus_tag="VW191_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9624075.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            4065..4925
                     /locus_tag="VW191_001313"
     CDS             4065..4925
                     /locus_tag="VW191_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEU9624076.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDITKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5387..6110
                     /locus_tag="VW191_001314"
                     /pseudo
     CDS             5387..6110
                     /locus_tag="VW191_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6301..8592
                     /gene="pcrA"
                     /locus_tag="VW191_001315"
     CDS             6301..8592
                     /gene="pcrA"
                     /locus_tag="VW191_001315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEU9624077.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8635..9315
                     /gene="radC"
                     /locus_tag="VW191_001316"
     CDS             8635..9315
                     /gene="radC"
                     /locus_tag="VW191_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU9624078.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9312..10001)
                     /locus_tag="VW191_001317"
     CDS             complement(9312..10001)
                     /locus_tag="VW191_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEU9624079.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(10019..10660)
                     /locus_tag="VW191_001318"
     CDS             complement(10019..10660)
                     /locus_tag="VW191_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU9624080.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10743..10958)
                     /locus_tag="VW191_001319"
     CDS             complement(10743..10958)
                     /locus_tag="VW191_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEU9624081.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(10966..11313)
                     /locus_tag="VW191_001320"
     CDS             complement(10966..11313)
                     /locus_tag="VW191_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEU9624082.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11318..12433)
                     /locus_tag="VW191_001321"
     CDS             complement(11318..12433)
                     /locus_tag="VW191_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU9624083.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETVFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLTGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12443..13402)
                     /locus_tag="VW191_001322"
     CDS             complement(12443..13402)
                     /locus_tag="VW191_001322"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9624084.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13515..14084)
                     /locus_tag="VW191_001323"
     CDS             complement(13515..14084)
                     /locus_tag="VW191_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEU9624085.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDMTDYELELEVENHEQGQQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            14212..14883
                     /locus_tag="VW191_001324"
     CDS             14212..14883
                     /locus_tag="VW191_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEU9624086.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRSITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            14867..15685
                     /locus_tag="VW191_001325"
     CDS             14867..15685
                     /locus_tag="VW191_001325"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU9624087.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            15682..16578
                     /locus_tag="VW191_001326"
     CDS             15682..16578
                     /locus_tag="VW191_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9624088.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            16622..17596
                     /gene="pta"
                     /locus_tag="VW191_001327"
     CDS             16622..17596
                     /gene="pta"
                     /locus_tag="VW191_001327"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEU9624089.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(17938..18237)
                     /locus_tag="VW191_001328"
     CDS             complement(17938..18237)
                     /locus_tag="VW191_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU9624090.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVISIDFNPAVIDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         5478 a         3374 c         4030 g         5650 t
ORIGIN      
        1 actatattgt tataaattga tttgaatttc aaaattaaat tgtttgattc ttatttcaat
       61 ttgttatagt atatctgatg tcaaagttct cggcgagtca aatagcgatt cccaagcctg
      121 actatcgtga ggtagcggat taaaatggtc tggggataga ccgttttaag tctgacgctg
      181 gaaataagaa ttgtcagaag aagggatagc gaactcgtgg ctctacgaac aggaacgtga
      241 taataaggcg tatatagcgg ataagagggc ctcaaactct aaagtccaaa aaggtagtcg
      301 taacctatat gcgtaaatta cgagagtaat tgaattcgta ctaagatttt ctattttcac
      361 tgtaagcttt taacgccctt atatcttgta tacacgagga aagatgtacg acttatcccg
      421 tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt cacggttatc
      481 tttaaaatag attgtctgat aggacggtat gtataaaacg cttctgtgaa ctgagagaag
      541 ggggagaagt tcttgctaaa atttagttga acagccgtat tccgatactt agataagaga
      601 tctagtctta gctcctactc agttttaggg gataaaaaag gggcaatagc gattcgagaa
      661 agattatact cttcgaaaat ctcttcaaat cacgtcaata tcgccttgtc gtatgtgtag
      721 gatactgact acgtcagttc catctacaac ctcaaaacag tgttttgagc aacctgcggc
      781 tagtttccta gtttgatctt tgattttcat tgagtattag taattcagtt actaactcgt
      841 caactctgat ttatccaata aaattgaaaa ggatggaaaa aaggataaat ttatgatata
      901 ctttattttg aagaccttat tagaaatctt gaaagagtat tgaaaactta gaatgagaaa
      961 aattgttatc aatggtggat taccactgca aggtgaaatc actattagtg gtgctaaaaa
     1021 tagtgtcgtt gccttaattc cagctattat cttggctgat gatgtggtga ctttggattg
     1081 cgttccagat atttcggatg tagccagtct tgtcgaaatc atggaattga tgggagctac
     1141 tgttaagcgt tatgacgatg tattggagat tgacccaaga ggtgttcaaa atattccaat
     1201 gccttatggt aaaattaaca gtcttcgtgc atcttactat ttttatggga gcctcttagg
     1261 ccgttttggt gaagcgacag ttggtctacc gggaggatgt gatcttggtc ctcgtccgat
     1321 tgacttacac cttaaggcgt ttgaagctat gggtgccact gctagctacg agggagataa
     1381 catgaagtta tctgctaaag atacaggact tcatggtgca agtatttaca tggatacggt
     1441 tagtgtggga gcaacgatta atacgatgat tgctgcggtt aaagcaaatg gtcgtactat
     1501 tattgaaaat gcagcccgtg aacctgagat tattgatgta gctactctct tgaataatat
     1561 gggtgcccat atccgtgggg caggaactaa tatcatcatt attgatggtg ttgaaagatt
     1621 acatgggaca cgtcatcagg tgattccaga ccgcattgaa gctggaacat atatatcttt
     1681 agctgctgca gttggtaaag gaattcgtat aaataatgtt ctttacgaac acctggaagg
     1741 gtttattgct aagttggaag aaatgggagt gagaatgact gtatctgaag acagcatttt
     1801 tgtcgaggaa cagtctaatt tgaaagcaat caatattaag acagctcctt acccaggctt
     1861 tgcaactgat ttgcaacaac cgcttacccc tcttttacta agagcgaatg gtcgtggtac
     1921 aattgtcgat acgatttacg aaaaacgtgt aaatcatgtt tttgaactag caaagatgga
     1981 tgcggatatt tcgacaacaa atggtcatat tttgtacacg ggtggacgtg atttacgtgg
     2041 ggccagtgtt aaagcgaccg acttaagagc tggggctgca ctagtcattg ctgggcttat
     2101 ggctgaaggt aaaactgaaa ttaccaatat cgagtttatc ttacgtggtt attctgatat
     2161 tatcgaaaaa ttacgtaatt taggagcgga tattagactt gttgaggatt aaaccgtaga
     2221 ggtgtttatg aatatttgga ccaaattagc aatgttttct ttttttgaaa cggatcgctt
     2281 gtatttgcgt cctttctttt ttagtgatag tcaggacttc cgcgagatag cttcaaatcc
     2341 agaaaatctt caatttattt tcccaacgca ggcaagtctg gaagaaagtc aatatgcact
     2401 ggccaattac tttatgaagt cccctttggg agtgtgggca atttgtgacc agaaaaatca
     2461 acaaatgatt ggttctatta aatttgagaa gttagatgaa atcaaaaaag aagctgagct
     2521 tggctatttt ttgagaaaag atgcttggtc gcaaggattt atgacagagg ttgttagaaa
     2581 aatttgtcag ctttcttttg aggaatttgg cttaaaacaa ttatctatca ttacccacct
     2641 tgaaaatgaa gctagccaaa gagttgctct taagtctgga tttagtttgt tccgtcagtt
     2701 taagggaagt gatcgttaca caagaaaaat gcgggattat cttgaatttc ggtatgtaaa
     2761 aggagagttc agtgagtaag catcaggaaa ttctaagcta tttggaggaa ttaccagtag
     2821 gtaaaagggt cagtgttcgt agcatttcga atcatctagg agttagtgat ggaacagcct
     2881 atcgggctat taaagaagct gaaaaccgtg gaattgtgga gacccgtcct agaagtggaa
     2941 caattcgtgt taaatcccag aaagttgcta tagatagatt aacgtttgct gaaattgcag
     3001 aagtgacttc ttctgaggtt ctggctgggc aagaaggttt agagagagaa tttagtaagt
     3061 tttcaattgg tgccatgact gaacaaaata tcttgtctta ccttcatgat ggggggctct
     3121 tgattgtcgg agaccgaacc cgtattcagt tgctagcctt ggaaaatgaa aatgcagttc
     3181 tggttacagg gggatttcag gttcatgatg atgtgcttaa actggccaat caaaaaggga
     3241 ttcctgttct aagaagtaag catgatacct ttaccgtcgc gaccatgatc aataaagcct
     3301 tgtcaaatgt ccaaatcaag actgatattc tgacagttga gaaactttat cgccctagtc
     3361 atgagtatgg ttttctgaga gagacagata cagttaaaga ttatttggac ttggttcgta
     3421 agaatcgtag cagccgtttc cctgttatca atcaacatca ggtcgttgtt ggcgttgtaa
     3481 ccatgagaga cgctggtgat aaatcaccaa gcacgacaat tgataaggtt atgtctcgta
     3541 gtctattttt ggttggatta tcgacaaata ttgccaatgt gagtcaacgg atgatcgcag
     3601 aagactttga aatggtacca gttgttcgaa gcaatcaaac tttgcttggc gttgtgacgc
     3661 gacgagatgt catggagaag atgagccgtt cccaagtttc ggctctacca actttttctg
     3721 agcagattgg acaaaagctc tcttatcacc atgatgaagt agttattaca gtggaaccct
     3781 ttatgctaga aaaaaatgga gttttggcta atggtgtatt ggcagaaatt ctgacccaca
     3841 tgacccaaga tttagttgtt aatagtggtc gcaatctcat tatcgagcag atgctgatct
     3901 actttttgca ggctgttcag atagatgata tattgcgcat tcaggcacgg attattcatc
     3961 atacgagacg gtcagctata attgattacg atatttatca tggtcaccag attgtttcaa
     4021 aagcaaatgt gactgttaaa attaattaga aactaggaga aaagatgata acattaaaat
     4081 cagctcgtga aatcgaagct atggacaagg ctggtgattt tctagcaagt attcatatag
     4141 gcttacgtga tttgattaaa ccaggcgtag atatgtggga agttgaagaa tatgtccgcc
     4201 gtcgttgtaa agaagaaaat ttccttccac ttcagattgg ggttgacggt gccatgatgg
     4261 actatcctta tgctacctgt tgctctctta acgatgaagt ggctcacgct ttccctcgtc
     4321 attatatctt gaaagatggt gatttgctca aagttgatat ggttttggga ggtcccattg
     4381 ctaaatccga cctgaatgtc tcaaaattaa acttcaacaa tgttgaacaa atgaaaaaat
     4441 acactcagag ctattctggt ggtttagcag actcatgttg ggcttatgct gttggtacac
     4501 cgtccgaaga agtcaaaaac ttgatggata taaccaaaga agctatgtac aagggtattg
     4561 agcaagctgt tgttggaaat cgtatcggtg atatcggtgc ggctattcaa gaatacgctg
     4621 aaagtcgtgg ttacggtgta gtgcgtgatt tggttggtca tggtgttggc ccaactatgc
     4681 acgaagaacc aatggttcct aactatggta ttgcaggtcg tggactccgt cttcgtgaag
     4741 gaatggtctt aaccattgaa ccaatgatca atacaggcga ttgggaaatt gatacagata
     4801 tgaaaactgg ttgggcgcat aagaccattg acggtggatt gtcatgtcag tatgaacacc
     4861 aatttgtcat tacgaaagat ggacctgtta tcttgactag ccaaggtgaa gaaggaactt
     4921 attaataaaa agtgaaaaga ctactggaag tttattttga taaaaaatcc agtagatctt
     4981 ttcataataa aacgcattgt atcaagtgtt aggagctgat atcatgcgtt tttctgcttt
     5041 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
     5101 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
     5161 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
     5221 tctgtttaac gttcgtattg ccaatcttaa atttaccttt tttagattag acgtcctgcg
     5281 aaacaaaata tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
     5341 atgatttaag cgtcttgttg gtgttcagcg taccactttt gaagagatgt tagctgtatt
     5401 aaaaacagct tatcaactta aacacgcaaa aggtggacga aaacctaaat tatgcctaga
     5461 agaccttctt atggccactc ttcaatatgt gcgagaatac cgcacttatg aagaaattgc
     5521 ggctgatttt ggtatccacg aaagcaactt aatccgtcgg agccaatggg ttgaagtaac
     5581 tcttgttcaa aatggtttta cgatttcaag aactcctctc agttctgagg acacggtaat
     5641 gattgatgcg acggaagtac aaatcaatcg ccctaaaaaa acaattagcg aatgattctg
     5701 gtaaaaagaa atttcacgct atgaaggctc aagcgattgt cacaagtcaa ggaagaattg
     5761 tttctttgga tatcactgtg aactattgtc atgatatgaa gttgttcaaa atgagtcgca
     5821 gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga
     5881 tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta acagttgaag
     5941 ataaagcctg taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca
     6001 aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat
     6061 tacgaatgaa tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga
     6121 agtctattga ggtattgagc tagtttatga aaaaattggg tgaaaagtcg agtgttttag
     6181 aaacccacag tgtagtattc tagtttcaat ccactatatt ttgctactcc ccgtaaagtt
     6241 tctattttcc ctgatttctg atataataga aatattgact tcaagagtaa ggaagagaag
     6301 atgaacgcat tattaaatgg aatgaatgac cgtcaggctg aggcggtgca aacgacagaa
     6361 ggtcccttgc taatcatggc aggggctggt tctggaaaga ctcgtgtttt gacccaccgt
     6421 atcgcttatt tgattgatga aaagctggtc aatccttgga atatcttggc cattaccttt
     6481 accaacaagg ctgcgcgtga gatgaaagag cgtgcttata gcctcaatcc agcgactcag
     6541 gactgtctga ttgcgacctt ccactccatg tgtgtgcgta ttttgcgtcg cgatgcggac
     6601 catattggct acaatcgtaa ttttacaatt gtggatcctg gtgaacagcg aacgctcatg
     6661 aaacgtattc tcaaacagtt gaacttggac cctaaaaaat ggaatgaacg aactattttg
     6721 gggaccattt ccaatgctaa gaatgatttg attgatgatg ttgcttatgc tgcccaagct
     6781 ggcgatatgt atacgcaaat tgtggcccag tgttatacag cctatcaaaa agaacttcgt
     6841 cagtctgaat ccgttgactt tgatgatttg attatgctga ccttgcgtct ctttgatcaa
     6901 aatcctgatg ttttgaccta ctaccagcaa aaattccaat acatccacgt tgatgagtac
     6961 caagatacca accacgctca gtaccaattg gtcaaactct tggcttcccg ttttaaaaat
     7021 atctgtgtgg ttggggatgc ggaccagtct atctacggtt ggcgtggtgc tgatatgcag
     7081 aatatcttgg actttgaaaa ggattacccc aaagccaagg ttgttttgtt ggaggaaaat
     7141 taccgctcaa ccaaaaccat tctccaagcg gccaatgagg ttattaaaaa taataaaaat
     7201 cgccgtccta aaaatctctg gactcaaaac gctgatgggg agcaaatcgt ttactatcgt
     7261 gccgatgatg agctggatga ggctgtattt gtagccagaa ccatcgatga acttagtcgc
     7321 agtcaaaact tccttcataa ggattttgca gttctctatc ggactaatgc ccagtcccgt
     7381 acaattgagg aagccctgct caagtctaac attccttata ccatggttgg cggaaccaaa
     7441 ttctacagcc gtaaggaaat tcgcgatatt attgcttatc tcaaccttat tgctaatttg
     7501 agtgacaata ttagttttga gcgtattatc aacgagccta aacgtggaat tggtctaggt
     7561 acagttgaga aaatccgtga ttttgcaaat ttgcaaaata tgtctatgct ggatgcttct
     7621 gctaatatta tgttgtctgg tatcaagggt aaggcagccc aatctatctg ggattttgcc
     7681 aatatgatgc ttgatttgcg ggagcagcta gaccacttaa gcattacaga gttggttgag
     7741 tccgtcctag aaaaaacagg ttatgtcgat attcttaacg cccaagcgac tctagaaagc
     7801 aaggcacggg ttgaaaatat cgaagagttt ctttctgtta cgaagaactt tgatgacacc
     7861 acggatgtga cagaagagga aactggtctg gacaaactga gtcgtttctt aaatgacttg
     7921 gctttgattg ccgacacaga ttcaggtagt caggagacat cagaagtgac cttgatgacc
     7981 ctgcatgctg ccaaaggtct cgaatttcca gttgtctttt tgattgggat ggaagaaaat
     8041 gtctttccac ttagtcgtgc gactgaagat ccagatgaat tagaagaaga gcgccgtcta
     8101 gcctatgtag gtatcacgcg tgcagagaaa attctctatc tgaccaatgc caactcacgc
     8161 ttgctttttg gtcgtaccaa ttataaccgt ccgactcgtt ttattaacga aatcagttca
     8221 gacttgcttg agtatcaagg tctggctcgt cctgcaaata caagctttaa ggcatcatat
     8281 agcagtggta gtatttcctt tggtcaaggt atgagtttgg ctcaggctct tcaagaccgt
     8341 aaacgcggtg ctgccccaaa atcaatccag tcaagcggtc ttccatttgg tcaatttaca
     8401 gctggcgcaa aaccagcatc tagcgaggca aattggtcca ttggtgatat tgctctccac
     8461 aagaaatggg gagagggaac cgttctggaa gtttcaggta gcggtgctag gcaggaattg
     8521 aaaatcaatt tcccagaagt aggtttgaaa aaacttttag ccagtgtggc tccaattgag
     8581 aaaaaaatct aattttccat ccttctcacg aataataaag tgaggaggat ttttatgtac
     8641 agtatttcat tccaagaaga ttcactatta ccaagagaaa ggctggccaa ggaaggagtt
     8701 gaagcgctta gtaaccaaga gttgctagct attttactca ggacaggaac acgtcaagct
     8761 agcgtttttg aaattgccca aaaagtcttg aacaatcttt caagcctaac ggatttgaaa
     8821 aaaatgaccc tgcaggaatt gcagagtttg tctggtattg ggcgtgttaa ggccatagaa
     8881 ttacaagcta tgattgaact ggggcatcgt attcacaaac acgagactct tgaaatggaa
     8941 agtattctca gcagtcaaaa gttggccaag aagatgcagc aggaattagg ggataaaaaa
     9001 caagagcacc tggtggcact ctatctcaat actcaaaatc aaatcatcca tcagcagacc
     9061 atttttatcg gttctgtaac tcgtagtatc gctgaaccgc gagagattct tcactatgca
     9121 atcaagcata tggcgacttc tcttatcttg gtccacaatc atccttcagg agcggtagcg
     9181 cctagccaaa atgatgatca tgtcactaaa cttgttaaag aagcctgcga actgatgggg
     9241 attgttctct tggaccattt gattgtctct cattctaatt actttagtta tcgtgaaaag
     9301 acagatttaa tctaaagttc attaacgaca tagtcaaaga gtttcttatc tttgggacga
     9361 ttttcaaaaa gaagttctgg atgccattgg acaccgagaa aggcgacatc atccgtactc
     9421 atgacagcct caatgatacc atctttagga tcatgagcca caacttttaa atttggtgct
     9481 aagtccttga tgctctggtg gtggaaggag ttgatatgag agatttctcc atagatttct
     9541 tggagaacgg tatctggttc tgttaccaag cgttgagttg tgtactcaac agaagaatcc
     9601 tgccaatggt cttcgatatc ttggtacaaa gttccaccca tggcaacgtt aaagagttgg
     9661 gtaccacggc agacagagaa aatgggcttt ttctgtttaa tagcttcctt gatgagggcc
     9721 agttcgaaga tatctctttg aaggtgataa tcatcgctat caatggtttt gggttcgcca
     9781 tagaattttg gatcgacatt ttgcccacct gtcaagatga gcttgtcaat caaactgata
     9841 tagtggcagg ccatttcttg atcaccaatc ggtaggatga tgggaatccc tccagcatct
     9901 ttaacgcctt caacaaagcc ttttgctgcg tagctcatca tgatgtcatc atctggatga
     9961 gttttttcgt ttcctgtaat cccaataact ggttttttca taaaatgatt ttcgctttct
    10021 aatcctcttt tcgcatgaag tagaggaggg tttggagttc acttgtcaaa tcgacatact
    10081 gaacgaccac gtcttttggt aaatgcagat ggactggtga aaaactgaga attcctttca
    10141 caccagcatc aaccaagaga ttagcaacct cttgtgactt gacgctggga acagttagga
    10201 tagcagtctt cacatcagca tccttgattt tatccttgat ctgagaaatc ccgtaaatgg
    10261 gaatcccgtc aggagtttgg gtaccgactt caggatggtc gtctaggtca aaggccatga
    10321 taatcttcat cttgttacgt tcgtggaagc ggtagtggag aagggcatgg cccatatttc
    10381 caataccaac cagcatgaca ttggtaatag agttgtcatt gagcaaatcg gcaaaaaatg
    10441 tcattagttt tttgacatca tagccaaaac cacgacgacc aagttcacca aaataggaaa
    10501 aatcacgacg tacggtcgct gaatcaatac cgatagcctc tgcaatttgc ttagagttgg
    10561 cacgttcaat cttttctgca tgaaatctct taaaaattcg atagtagaga gagagtcttt
    10621 ttgctgtagc ttttggaata gcaaactgtt tatctttcac aaaatcacaa cctttctatt
    10681 cttctatttt atagaaacat tgtgaaaaaa tcaacaaaaa taagaaaaaa ctaagaaaaa
    10741 tcttagtttt gatgtaaaaa atctgcatga gatagaaaac ggtagaggtc tccgaccagc
    10801 ccctgataaa cttttttgcc cctaaaagtc agagaagtca cataaagtgt atctggtaag
    10861 gttacacatc ctgacaaagt caacatgaga gcctcatgat cctcatactt gagagtacgc
    10921 tctacatgat agcagtcctt ataggtcagt tcaaacattt tggctctatc tttccgattt
    10981 tgtaaagaca ccacgttcta ccaagctatc catgaggaag tagaattttt cctgatgaat
    11041 atggtggtct tctgatttga aaatatcaac tagacgaagg ccaaacttgt cagtgatatt
    11101 gattttagcc cctgtaagtt ccttgttaat gatgattttg agttggaagc cttcaccgct
    11161 gtttggcact ttttccaaaa ggcgagtcag ttcatagtta ccaaccttag tttcaaaaaa
    11221 ggtgttatct ttgagggtga attttttaac agaagggcta agagtgtaat cgtaacgaca
    11281 attttttaac tgaatgattt tttcaaatgc catatggcta acctccgata atttctttta
    11341 aggtttttgc gagggtttgt aggtcttcaa cggtattttg tggcgacaaa ctgatgcgaa
    11401 gggattcctt caagcgttct gaatttgcgc catacatggc tttaagaaca tggctggatt
    11461 ggacaacgcc tgcagtacag gctgagccag tagagattga aattccagtt aaatctagcc
    11521 gaaggagtaa gaggtcattt ttctgaccag gaaatccaat attgagaaca taagggagat
    11581 gatgttttcc tctattcaag taatactgaa tgccctccag ctctgccaga aagacagttt
    11641 ctagattttg tacatgttga aaatgttctt cttgtttttc taggtcttct tttagggctg
    11701 caaccatgcc tacgatggca ggcagatttt cagttcctgc acgttttttc tgttcctgat
    11761 ctccgccatg tagataggaa tcaaagtcca tgctagatgc gtagagaaaa ccgattccct
    11821 taggaccatg gaatttgtgg gcagaagcag tgagaaaatc aatgcccaat tcttctgaat
    11881 gaattgggat tttaccaata gcctgaactg catcaacatg ataggcagca gggtgttgct
    11941 tgagtatttg gccaatttca gcgatgggaa gtaggtttcc tgtctcatta ttggcaaaca
    12001 tagtagaaac caaaatcgta tcgtcacgta aagccttttg aatttgctgg gctgtgattt
    12061 cttgattttc tggctggata atggttgctt caaacccaaa gtgttgaacc aagtaatcaa
    12121 ttgtttcaag gacagcatgg tgctcgatgg cagttgtgat gatatgtttt ccttgttctt
    12181 ggtgacgaag acagtagcca atgatggtag tattattgcc ttcagtccca ccagaagtga
    12241 aaaagatatg ttgaggtttt gtccttagta actgggctag ttcctgacgg gcttctcgca
    12301 agagtttgcc agcttgacga ccatgaccat gaatactaga aggatttccg tgggtttctt
    12361 gcataacctt ggtcatagct gaaatagcaa ctgctgacat aggagtcgtt gcagcattgt
    12421 ccaaataaat caaagaatca ccttatttct ttttattgta ggcaaagagt gggctgactg
    12481 gttttctttc gtgaatacgg acgatagcat caccaattaa ctcactagca gtgatgtagc
    12541 atacattttt aggagttttt tcttttgttg ctactgaatc agtcacaaga atttctttaa
    12601 tattagtatt gtcaagaagc tcagcagctc cctcgacgaa gagaccgtgg ctagaaacag
    12661 cataaatttc tgtagctcct tcacgttcaa cgattttaga agcttcagag aaggtacgtc
    12721 ctgtatttaa aatatcatca atcaagatag ctttcttacc ttcaacatca ccaataatat
    12781 aaccttcgtt acgagttgca tcgtcttgag ggtagtcgat aatggcgata ggagcatcaa
    12841 gatattcagc caggctacgc gcacgtttga cacctgaatt tttagggcta acgacaacaa
    12901 catctgaacc aagcaatcct ttatcgcagt aatgttttgc gaatagggga acagtgaaaa
    12961 gattatccac tggaatatca aagaaacctt gaacctgaac ggcatgcaaa tcaagagtca
    13021 ggatacgatc aactccagcc ttaaccagca tattggcaac tagttttgct gtaagtggct
    13081 cacgaggaca agcaatgcgg tcttgacgtg catagccaaa atatggaagg acaacgttga
    13141 tactgtgggc acttgcacgc acacaagcat cgaccatgat taacaattcc attaggtggt
    13201 tgttgacagg gaaacttgtt gattggatga tgtaaacatc ataaccacgg acactttctt
    13261 cgatatttac ttggatttct ccgtctgaaa attgacgtga tgatagtttt ccaagtggga
    13321 caccaacagc ttgggcaatt ttttgtgcaa tctcttggtt agagttgagt gcgaaaagtt
    13381 tcatgttttt tctatctgac attatagacc gtcctctgta aactttataa atcctagtta
    13441 tatttacctt acatatatga actggggttt gtgtattttt atcttttcta ttttaccaaa
    13501 aaatggagat tatttcagct atttttcata cttttgacaa atcgaaccaa ttttgaagga
    13561 gctttttggt aggaaatctg atttttctct aaaaattgtc ggaaatcctg ttggccttgc
    13621 tcatgatttt ccacttcaag ctccaattcg taatctgtca tatcaaagta tcggctctga
    13681 tccagtgcca tgagaccaat agctgttttc atttcatagc gaagcgttgt tagacaacca
    13741 agaacctgcc agttcttact ttggatacca tgtttcgcca attcatccag tactagccct
    13801 tgaggaagtt cttccttact cagatagttc tcagcatctt ttagttgcaa tttttggttg
    13861 tattccatgt ttccaacact ctgcgggact ttgagtgtca actcagccca gtcttcaaag
    13921 gttcgaatgc gcatagcgac tttcttttct cgcagttcaa aatcaggcgt gtcgatgtag
    13981 taatttgttt gaagaacagg agtgacacct gtgaactggt cttttagacg attgtattca
    14041 tcttttttca atagtgtttt caattcaatt tctaaatgtt tcatttttct tacctttttt
    14101 tatcgttgaa agcggattta tggtataata agcattgtat ttattgtata tgaatctgga
    14161 gaaaaaatca aagatatttt tgacggataa tatgagaaca agggagaata tatgacctta
    14221 gaatgggaag aatttctaga tccttacatt caagctgttg gtgagttaaa gattaaactt
    14281 cgtggtattc gtaagcaata tcgtaagcaa aataagcatt ctccaattga gtttgtgacc
    14341 ggtcgagtca agccaattga gagcatcaaa gaaaaaatgg ctcgtcgtag cattacttat
    14401 gcgaccttgg aacacgattt gcaggatatt gctggcttac gtgtgatggt tcagtttgta
    14461 gatgacgtca aggaagtagt ggatattttg cacaagcgtc aggatatgcg aatcatacag
    14521 gagcgagatt acattactca tagaaaagca tcaggctatc gttcctatca tgtggtagta
    14581 gaatatacgg ttgataccat caatggagct aagactattt tggcagaaat tcaaattcgt
    14641 actttggcca tgaatttctg ggcaacgata gaacattctc tcaactacaa gtaccaaggg
    14701 gatttcccag atgagattaa gaagcgactg gaaattacag ctagaatcgc ccatcagttg
    14761 gatgaagaaa tgggtgaaat tcgtgatgat atccaagaag cccaggcact ttttgatcct
    14821 ttgagtagaa aattaaatga cggtgtagga aacagtgacg atacagatga agaatacagg
    14881 taaacgaatt gatctgatag ccaatagaaa accgcagagt caaagggttt tgtatgaatt
    14941 gcgagatcgt ttgaagagaa atcagtttat actcaatgat accaatccgg atattgtcat
    15001 ttccattggc ggggatggta tgctcttgtc ggcctttcat aagtacgaaa atcagcttga
    15061 caaggtccgc tttatcggtc ttcatactgg acatttgggc ttctatacag attatcgtga
    15121 ttttgagttg gacaagctag tgactaattt gcagctagat actggggcaa gggtttctta
    15181 ccctgttctg aatgtgaagg tctttcttga aaatggtgaa gttaagattt tcagagcact
    15241 caacgaagcc agcatccgca ggtctgatcg aaccatggtg gcagatattg taataaatgg
    15301 tgttcccttt gaacgttttc gtggagacgg gctaacagtt tcgacaccga ctggtagtac
    15361 tgcctataac aagtctcttg gcggtgctgt tttacaccct accattgaag ctttgcaatt
    15421 aacggaaatt gccagcctta ataatcgtgt ttatcgaaca ctgggctctt ccattattgt
    15481 gcctaagaag gataagattg aacttattcc aacaagaaac gattatcata ctatttcggt
    15541 tgacaatagc gtttattctt tccgtaatat tgagcgtatt gagtatcaaa tcgaccatca
    15601 taagattcac tttgtcgcga ctcctagcca taccagtttc tggaaccgtg ttaaggatgc
    15661 ctttatcggt gaggtggatg aatgaggttt gaatttatcg cagatgaaca tgtcaaggtt
    15721 aagacctttt taaaaaagca cgaggtttct aagggattgc tggccaagat taagtttcga
    15781 ggtggagcta ttctggtcaa taatcaaccg caaaatgcaa cgtatctatt ggacgttgga
    15841 gactacgtta ccatttacat tcccgctgag gaaggctttg aaaccttgga ggctattgag
    15901 catccattag atattctcta tgaggatgac cactttctag tcttgaataa accctatgga
    15961 gtggcttcta ttcctagtgt caatcactct aataccattg ccaattttat caagggttac
    16021 tatgtcaagc aaaattatga aaatcagcag gttcacattg ttaccagact agatagggac
    16081 acttctggct tgatgctctt tgccaagcac ggttatgccc atgcacgatt agacaagcag
    16141 ttgcagaaga aatctatcga gaaacgctac tttgctttgg ttaagggaga tggacatttg
    16201 gagccagaag gggaaattat tgctccgatt gcgcgtgatg aagattccat tattaccaga
    16261 cgagtggcta aaggcggaaa gtatgcccat acttcataca agattgtagc ttcttatgga
    16321 aatattcact tggtctatat tcacctgcac actggtcgaa cccatcaaat ccgagtccat
    16381 ttttctcata tcggttttcc tttgctggga gatgatttgt atggtggtag tctggaagat
    16441 ggtattcaac gtcaggctct gcattgccat tacctatcct tttatcatcc atttttagag
    16501 caagacttgc agttagaaag tcccttgccg gatgatttca gtaaccttat tacccagtta
    16561 tcaactaata ctctataaaa actgtctcag agtataatta ttatcttaaa ggagaaaact
    16621 catggaagtt tttgaaagtc tcaaagccaa ccttgttggt aaaaatgctc gtatcgttct
    16681 ccctgaaggg gaagagcctc gtattcttca agcaacaaaa cgcttagtaa aagaaacaga
    16741 agtgattcct gttttgcttg gaaatcctga aaaaattaaa atttatcttg aaattgaagg
    16801 aatcatggat ggttatgagg tcatcgaccc tcaacattat cctcaatttg aagaaatggt
    16861 ttctgccttg gtggagcgtc gcaagggcaa aatgactgaa gaagatgtac gcaaggtttt
    16921 ggttgaagat gtcaactact ttggtgtgat gttggtttac ttgggcttgg ttgatggaat
    16981 ggtgtcagga gcgattcact caacagcttc aacagttcgc ccagctctac aaatcatcaa
    17041 aactcgtcca aatgtaactc gtacttcagg agccttcctc atggttcgtg gtacggaacg
    17101 ttacctattt ggagactgtg ccattaatat caatccagat gcagaagcct tggctgaaat
    17161 tgccatcaac tcagcaatca cagctaagat gtttggcatc gaacctaaaa ttgccatgtt
    17221 gagctattct actaaaggtt cagggtttgg tgaaagcgtt gataaggtcg ttgaagcaac
    17281 taaaattgct cacgacttgc gtcctgacct tgaaatcgat ggtgagttgc aatttgatgc
    17341 ggcctttgtt cccgaaactg cagctctgaa agctccggga agtacagtag ctggtcaagc
    17401 aaatgtcttc atcttcccag gtatcgaggc aggaaatatc ggttacaaga tggctgaacg
    17461 cctgggtggc tttgcggctg taggacctgt tttgcaaggt ttaaacaagc cagttaatga
    17521 tctttctcgt ggatgtaatg cagatgatgt ttacaagttg accctcatca cagcagctca
    17581 agcagttcat caatagtgaa aactataaag tgatatacta tgctatactg tagttatgaa
    17641 actatgtacg aaaagcactg ccattaattc ctgagaacta aattactgat tggtatcaaa
    17701 aaggaaaact tccaagcgat gatatcctgt ctatacacga cctatagaaa tctgtaatat
    17761 acatatccgt aaaacgataa attccctttt tgattttaaa tgagtatgga aagagaattt
    17821 tttcatgaga aaacgcataa tattaagtgt cttgacacct gctattatgc gttttttata
    17881 atttttgcac ctttcttagc ctttgttatc gacaagcatt gttttagctc cttgttttta
    17941 gaatggcaat ttcccagcga aagcacccag ttttttcttg gtagtttcat cgatttgttc
    18001 aagagcagag ttgatggctt gaacggtcat atcagaaaga gtttcaaggt cctctgggtc
    18061 aatgacagct ggattgaaat caatgctgat aactttctta tctccagtta aggtcgcttg
    18121 gacaagatct tgagcagatt tgccaacaaa ttgcatagca gcaagttcag cttggctttg
    18181 ttccatttgt ttttgaagtt tttgtgcttg acgcatcatg ttttgcatgt tcatcataag
    18241 atttctaaag ttcctttcat ctcaaggaga taatccttgt tgttaacaag tgcctgattt
    18301 tctctcctaa ttgttattag tagcatagcc atcaagcgca agtattgatt ttattatacc
    18361 atttttttaa gacatagtct tttattggtg agagttattt atgtcaagga gtgattagaa
    18421 ataaattaaa ataatgattt ttgctaagag gggctctatt ctagtttcat ggaaatgatt
    18481 ttactaaagg ttcgtatgct ttgtttgcaa acatgttacc tctatctatt tt
//
LOCUS       DAVKJU010000040        18448 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVKJU010000040 DAVKJU010000000
VERSION     DAVKJU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.040"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1077
                     /gene="dnaN"
                     /locus_tag="VW191_001329"
     CDS             <1..1077
                     /gene="dnaN"
                     /locus_tag="VW191_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU9624091.1"
                     /translation="AISSKNAIPILSTVKIDVTNEGITLIGSNGQISIENFISQKNED
                     AGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQ
                     YPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVAT
                     DSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENI
                     SFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKD
                     GVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAV
                     RPFTLVPADTDEDFMQLITPVRTN"
     gene            1142..1336
                     /locus_tag="VW191_001330"
     CDS             1142..1336
                     /locus_tag="VW191_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEU9624092.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1420..2535
                     /gene="ychF"
                     /locus_tag="VW191_001331"
     CDS             1420..2535
                     /gene="ychF"
                     /locus_tag="VW191_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU9624093.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLIESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYESEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2606..3175
                     /locus_tag="VW191_001332"
     CDS             2606..3175
                     /locus_tag="VW191_001332"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU9624094.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     KADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3176..6685
                     /gene="mfd"
                     /locus_tag="VW191_001333"
     CDS             3176..6685
                     /gene="mfd"
                     /locus_tag="VW191_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU9624095.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTNICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            6753..7019
                     /locus_tag="VW191_001334"
     CDS             6753..7019
                     /locus_tag="VW191_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEU9624096.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7012..7380
                     /locus_tag="VW191_001335"
     CDS             7012..7380
                     /locus_tag="VW191_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU9624097.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7500..8768
                     /locus_tag="VW191_001336"
     CDS             7500..8768
                     /locus_tag="VW191_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEU9624098.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8765..10042
                     /gene="tilS"
                     /locus_tag="VW191_001337"
     CDS             8765..10042
                     /gene="tilS"
                     /locus_tag="VW191_001337"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU9624099.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPKLEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKTQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10046..10588
                     /gene="hpt"
                     /locus_tag="VW191_001338"
     CDS             10046..10588
                     /gene="hpt"
                     /locus_tag="VW191_001338"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU9624100.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEVDYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10604..12562
                     /gene="ftsH"
                     /locus_tag="VW191_001339"
     CDS             10604..12562
                     /gene="ftsH"
                     /locus_tag="VW191_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU9624101.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQNPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            12684..13163
                     /locus_tag="VW191_001340"
     CDS             12684..13163
                     /locus_tag="VW191_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU9624102.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            13257..13328
                     /locus_tag="VW191_001341"
     tRNA            13257..13328
                     /locus_tag="VW191_001341"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13290..13292,aa:Glu,seq:ttc)
     gene            13581..15126
                     /locus_tag="VW191_001342"
     rRNA            13581..15126
                     /locus_tag="VW191_001342"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            15178..15250
                     /locus_tag="VW191_001343"
     tRNA            15178..15250
                     /locus_tag="VW191_001343"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15211..15213,aa:Ala,seq:tgc)
     gene            15375..18276
                     /locus_tag="VW191_001344"
     rRNA            15375..18276
                     /locus_tag="VW191_001344"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            18353..>18448
                     /gene="rrf"
                     /locus_tag="VW191_001345"
     rRNA            18353..>18448
                     /gene="rrf"
                     /locus_tag="VW191_001345"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6056 a         3033 c         4240 g         5119 t
ORIGIN      
        1 gctattagtt ctaaaaatgc cattcctatt ttatcaacag taaaaattga cgtgaccaat
       61 gaaggtatta ctttaattgg ttcaaatggt caaatttcaa ttgaaaattt tatttctcaa
      121 aaaaatgaag atgctggttt gttaattact tctttaggtt cgatccttct tgaagcttct
      181 ttctttatca atgtagtatc tagtttacct gatgtaactc ttgattttaa agaaattgaa
      241 caaaatcaaa ttgttttaac cagtggcaaa tcagaaatta ccctaaaagg aaaagatagc
      301 gaacaatatc cacgaatcca agaaatttca gcaagcactc ctttaatact tgaaacaaaa
      361 ttactcaaga aaattattaa tgaaacagcc tttgctgcaa gtacacaaga gagtcgtccg
      421 attttaacag gtgtccactt cgtattgagt caacacaaag agttaaaaac agttgcaaca
      481 gactctcatc gcctaagcca gaaaaaattg actcttgaaa aaaatagtga tgattttgat
      541 gtcgtaattc ctagccgttc tctacgcgaa ttttcagcgg tatttacaga tgatatcgaa
      601 actgtagaga ttttctttgc caataatcaa atcctcttta gaagcgaaaa tattagcttc
      661 tatactcgtc tcctagaagg aaactatcct gatacagatc gcttgattcc aacagacttt
      721 aacactacta ttacttttaa tgtggtaaac ttacgccagt caatggagcg tgcccgtctt
      781 ttatcaagtg cgactcaaaa tggtactgtg aaacttgaaa ttaaggatgg ggttgttagc
      841 gcccatgttc actctccaga agttggtaaa gtaaacgaag aaatcgatac tgatcaggtt
      901 actggtgaag atttgaccat tagtttcaac ccaacttact tgattgattc tcttaaagct
      961 ttaaatagcg aaaaggtgac tattagcttt atctcagctg ttcgtccatt tactcttgtg
     1021 ccagcagata ctgacgaaga cttcatgcag ctcattacac cagttcgtac aaattaagtg
     1081 aaagaggttg agcctggctc gcctctttta tgatataatc gaaaaagaaa aggagagtag
     1141 tatgtatcaa gttggaaatt ttgttgagat gaaaaaatca cacgcttgta caatcaagtc
     1201 gactggtaaa aaggctaatc gttgggaaat tacacgtgta ggagcagata tcaaaataaa
     1261 atgcagtaat tgtgagcatg ttgtcatgat ggggcgatat gattttgagc gaaaaatgaa
     1321 taaaattatt gactgagaac ccttagttag agggttagca ctttatccct ttttgtgtta
     1381 taatattagg gattgaaatg aaaacggaga atgagaaata tggctttgac agcaggtatc
     1441 gttggtttgc caaacgttgg taaatcaaca ctatttaatg caattacaaa agcaggagca
     1501 gaggcagcaa actacccatt tgcgactatt gatccaaatg ttggaatggt ggaagttcca
     1561 gatgaacgcc tacaaaaact aactgaaatg ataactccta aaaagacagt tcccacaaca
     1621 tttgaattta cggatattgc agggattgta aaaggagctt caaaaggaga agggctaggg
     1681 aataaattct tggccaatat tcgtgaagta gatgcgattg ttcacgtagt tcgtgctttt
     1741 gatgatgaaa atgtgatgcg cgagcaagga cgtgaagacg cctttgtgga tccacttgca
     1801 gatattgaca ccattaacct agaattgatt cttgcagact tagaatcagt caataaacga
     1861 tatgcgcgtg tagaaaagat ggcacgtacg caaaaagata aagaatcagt agcagaattc
     1921 aatgttcttc aaaagattaa accagtccta gaagacggga aatcagctcg taccattgaa
     1981 tttacagatg aggaacaaaa ggttgtcaaa ggtcttttcc ttttgacgac taaaccagtt
     2041 ctttatgtag ctaatgtgga cgaggatgtg gtttcagaac ctgactctat cgactatgtc
     2101 aaacaaattc gtgaatttgc agcgacagaa aatgctgaag tagtcgttat ttctgcgcgt
     2161 gctgaggaag aaatttctga attggatgat gaagataaaa aagagtttct tgaagccatt
     2221 ggtttgatag aatcaggtgt agataagttg acgcgtgcag cttaccactt gcttggattg
     2281 ggaacttact tcacagctgg tgaaaaagaa gttcgcgctt ggactttcaa acgtggtatg
     2341 aaggctcctc aagcagctgg tattatccac tcagactttg aaaaaggctt tattcgtgca
     2401 gtaaccatgt catatgagga tctagtgaaa tacgaatctg aaaaggccgt aaaagaagct
     2461 ggacgcttgc gtgaagaagg aaaagaatat atcgttcaag atggcgatat catggaattc
     2521 cgctttaatg tctaaaaatt aataaatggt gtcaattagg ttggaaaaaa attccaaccc
     2581 ttttggcttt tgaaaggaaa aataaatgac caaattactt gtaggcttgg gaaatccagg
     2641 ggataaatat tttgaaacaa aacacaatgt tggttttatg ttgattgatc aactagcgaa
     2701 gaaacagaat gtcactttta cacacgataa gatatttaaa gctgacctag catccttttt
     2761 cctaaatgga gaaaaaattt atctggttaa accaacgacc tttatgaatg aaagtggaaa
     2821 agcagttcat gctttattaa cttactatgg tttggatatt gacgatttac ttatcattta
     2881 cgatgatctt gacatggaag ttgggaaaat tcgtttaaga gcaaaaggct cagcaggtgg
     2941 tcataatggt atcaagtcta ttattcaaca tataggaact caggtcttta accgtgttaa
     3001 gattggaatt ggaagaccta aaaatggtat gtcagttgtt catcatgttt tgagtaagtt
     3061 tgacagggat gattatatcg gtattttaca gtctgttgac aaagttgacg attctgtaaa
     3121 ctactattta caagagaaaa attttgagaa aacaatgcag aggtataacg gataaatggt
     3181 gaccttatta gatttattct cagaaaatga tcagattaaa aaatggcatc aaaatttaac
     3241 tgataagaaa agacaactaa tacttggttt atcaacatct actaaggctc ttgcaattgc
     3301 aagcagttta gaaaaagaag ataggattgt gttattgacg tcaacttatg gagaagcaga
     3361 aggacttgtt agtgatctta tatctatctt gggtgaggaa ctcgtctatc catttttggt
     3421 agatgatgct cctatggtgg agtttttgat gtcttcacag gaaaaaatta tttcacgggt
     3481 tgaagccttg cgttttttga ctgattcatc taagaaaggg attttagttt gtaatatcgc
     3541 agcaagtcga ttgattttac cgtctcccaa tgcattcaaa gatagtattg taaaaatctc
     3601 agttggtgaa gaatatgatc aacacgcgtt tatccatcag ttaaaggaaa atggctatcg
     3661 aaaagttact caagtacaaa ctcagggcga atttagtctt cgaggagata ttttagatat
     3721 ttttgaaata tcccagttag aaccttgtcg aattgagttt tttggtgatg aaattgatgg
     3781 tatcaggtca tttgaagtag aaacacaatt atcgaaagaa aataagacag aactcactat
     3841 ctttccagct agtgatatgc ttttgagaga aaaggattat caacgaggac agtcagcttt
     3901 agaaaaacaa atttcaaaaa ctttatcacc tattttgaaa tcatacctag aagaaattct
     3961 ttcaagtttt caccaaaaac aaagtcatgc agactctcgg aagtttttat ctttgtgcta
     4021 tgataagaca tggactgtct ttgattatat tgaaaaagat actccaatat tctttgatga
     4081 ttatcaaaaa ttgatgaatc agtatgaagt ctttgaaaga gacttagcgc agtactttac
     4141 agaagaatta cagaatagta aagcattttc tgatatgcag tatttttctg atattgaaca
     4201 aatctataaa aaacaaagtc cagtgacctt tttctctaat cttcaaaagg gtttaggaaa
     4261 tctcaaattt gacaaaattt atcaattcaa tcaatatcct atgcaggaat ttttcaatca
     4321 gttttctttt ctaaaagaag aaattgaacg atataaaaaa atggattaca ccattattct
     4381 gcagtctagc aattcaatgg gaagtaaaac attggaggat atgttagagg aatatcagat
     4441 taaattggat tctagagata agacaaatat ctgtaaagaa tctgtaaact taatagaggg
     4501 taatctcaga catggttttc attttgtaga tgaaaagatt ttattgataa ctgaacatga
     4561 gatttttcaa aagaaattaa agcgtcgttt tcgaagacaa catgtttcaa atgcagagag
     4621 attaaaagat tacaatgaac ttgaaaaagg ggactatgtt gtccatcata tccatgggat
     4681 tggtcaatat ctaggaattg aaaccattga aatcaaggga attcatcgcg attatgtcag
     4741 tgtccaatac caaaatggtg atcaaatttc tatccccgtg gaacagattc atctactgtc
     4801 caaatatatt tcaagtgatg gtaaagctcc aaaactcaat aaattaaatg acggtcattt
     4861 taaaaaggcc aagcaaaagg ttaagaacca ggtagaggat atagctgatg atttaatcaa
     4921 actctactct gaacgtagtc agttgaaggg ttttgctttc tcagctgatg atgatgatca
     4981 agatgccttt gatgatgctt tcccttatgt tgaaacggat gatcaacttc gtagtattga
     5041 ggaaatcaaa agggatatgc aggcttctca gccaatggat cgacttttag ttggggatgt
     5101 tggttttgga aagactgaag ttgctatgcg tgcagccttt aaagcagtca atgatcacaa
     5161 acaggttgtc attctagttc cgacgacggt tttagcgcaa cagcactata cgaattttaa
     5221 ggaacgattc caaaattttg cagttaatgt tgatgtgttg agtcgcttta gaagtaaaaa
     5281 agagcagact gcaacacttg aaaaattgaa aaacggtcaa gtcgatattt tgattggaac
     5341 acatcgtgtt ttgtcaaaag atgttgtgtt tgctgatttg ggcttgatga ttattgatga
     5401 ggaacagcga tttggtgtca agcataagga aactttgaaa gaactgaaga aacaagtgga
     5461 tgtcctaacc ttgaccgcta cgccaatccc tcgtaccctc catatgtcta tgctgggaat
     5521 cagagatttg tctgttattg aaactccgcc gactaatcgc tatcctgttc agacctatgt
     5581 tttggaaaag aatgatagtg tcattcgtga tgctgtcttg cgtgaaatgg agcgtggagg
     5641 tcaagtttac tatctttaca acaaagttga cacaattgtt cagaaggttt cagaattaca
     5701 ggagttgatt ccggaggctt cgattggata tgttcatggt cgaatgagtg aagtccagtt
     5761 ggaaaatact ctattagact ttattgaggg acaatacgat atcttggtga cgactactat
     5821 tattgagaca ggggtggaca ttccaaatgc taatacttta tttattgaaa atgcggacca
     5881 tatgggcttg tcaaccttat atcagttaag aggaagagtc ggtcgtagta atcgtattgc
     5941 ttatgcttat ctcatgtatc gtccagaaaa atcaatcagt gaagtctctg aaaagagatt
     6001 agaagcgatt aaaggattta cagaattggg ctctggcttt aagattgcaa tgcgagatct
     6061 ttcgattcgt ggagcaggaa atcttttagg aaaatcccag tctggtttca ttgattctgt
     6121 tggttttgaa ttgtattcgc agttattaga ggaagctatt gctaaacgaa acggtaatgc
     6181 taacgctaac acaagaacca aagggaatgc tgagttgatt ttgcaaattg atgcctatct
     6241 tcctgatact tatatttctg atcaacgaca taagattgaa atttacaaga aaattcgtca
     6301 aattgacaac cgtgtcaatt atgaagagtt acaagaggag ttgatagacc gttttggaga
     6361 atacccagat gtagtagcct atcttttaga gattggtttg gtcaaatcat acttggacaa
     6421 ggtctttgtt caacgtgtgg aaagaaaaga taataaaatt acaattcaat ttgaaaaagt
     6481 cactcaacga ctgtttttag ctcaagatta ttttaaagct ttatccgtaa cgaacttaaa
     6541 agcaggcatc gctgagaata agggattaat ggagcttgta tttgatgtcc aaaataagaa
     6601 agattatgaa attttagaag gtctgctgat ttttggagaa agtttattag agataaaaga
     6661 gtttaaggaa gaaaattcca tttgatattt ttcttctata aaatagataa aaatagataa
     6721 aaatggtaca ataataaatt gaggtaataa ggatgagatt agataaatat ttaaaagtat
     6781 cgcgaattat caagcgtcgt acagtcgcaa aggaagtagc agataaaggt agaatcaagg
     6841 ttaatggaat cttggccaaa agttcaacgg acttgaaagt taatgaccaa gttgaaattc
     6901 gctttggcaa taagttgctg cttgtaaaag tactagagat gaaagatagt acaaaaaaag
     6961 aagatgcagc aggaatgtat gaaattatca gtgaaacacg ggtagaagaa aatgtctaaa
     7021 aatattgtac aattgaataa ttcttttatt caaaatgaat accaacgtcg tcgctacctg
     7081 atgaaagaac gacaaaaacg gaatcgtttt atgggggggg tattgatttt gattatgcta
     7141 ttatttatct tgccaacttt taatttggcg cagagttatc agcaattact ccaaagacgt
     7201 cagcaattag cagacttgca aactcagtat caaactttga gtgatgaaaa ggataaggaa
     7261 acagcatttg ctaccaagtt gaaagatgaa gattatgctg ctaaatatac acgagcgaag
     7321 tactattatt ctaagtcgag ggaaaaagtt tatacgattc ctgacttgct tcaaaggtga
     7381 taaaatggaa aatttattag acgtaataga gcaatttttg agtttgtcag atgaaaagct
     7441 ggaagaattg actgataaaa atcaattatt gcgtttacaa gaagaaaagg aaaggaagaa
     7501 tgcgtaaatt cttaattatt ttgttgctac caagtttttt gaccatttca aaagtcgtta
     7561 gcacagaaaa agaagtcgtc tatacttcga aagaaattta ttacctttca caatctgact
     7621 ttggtattta ttttagagaa aaattaagtt ctcccatggt ttatggagag gttcctgttt
     7681 atgcgaatga agatttagta gtggaatctg ggaaattgac tcccaaaaca agttttcaaa
     7741 taaccgagtg gcgcttaaat aaacaaggaa ttccagtatt taagctatca aatcatcaat
     7801 ttatagctgc ggacaaacga tttttatatg atcaatcaga ggtaactcca acaataaaaa
     7861 aagcatggtt agaatctgac tttaaactgt acaatagtcc ttatgattta aaagaagtga
     7921 aatcatcctt atcagcttat tcgcaagtat caatcgacaa gaccatgttt gtagaaggaa
     7981 gagaatttct acatattgat caggctggat gggtagctaa agaatcaact tctgaagaag
     8041 ataatcggat gagtaaagtt caagaaatgt tatctgaaaa atatcagaaa gattctttct
     8101 ctatttatgt taagcaactg actactggaa aagaagctgg tatcaatcaa gatgaaaaga
     8161 tgtatgcagc cagcgttttg aaactctcct atctctatta tacgcaagaa aaaataaatg
     8221 agggtcttta tcagttagat acgactgtaa aatacgtatc tgcagtcaat gattttccag
     8281 gttcttataa accagaggga agtggtagtc ttcctaaaaa agaagataat aaagaatatt
     8341 ctttaaagga tttaattacg aaagtatcaa aagaatctga taatgtagct cataatctat
     8401 tgggatatta catttcaaac caatctgatg ccacattcaa atccaagatg tctgccatta
     8461 tgggagatga ttgggatcca aaagaaaaat tgatttcttc taagatggcc gggaagttta
     8521 tggaagctat ttataatcaa aatggatttg tgctagagtc tttgactaaa acagattttg
     8581 atagtcagcg aattgccaaa ggtgtttctg ttaaagtagc tcataaaatt ggagatgcgg
     8641 atgaatttaa gcatgatacg ggtgttgtct atgcagattc tccatttatt ctttctattt
     8701 tcactaagaa ttctgattat gatacgattt ctaagatagc caaggatgtt tatgaggttc
     8761 taaaatgagg gaaccagatt ttttaaatca ttttctcaag aagggatatt tcaaaaagca
     8821 tgctaaggcg gttctagctc tttctggtgg attagattct atgtttctat ttaaggtatt
     8881 gtctacttat caaaaagagt tagagattga attgattctg gctcatgtga atcataagca
     8941 gagaattgaa tcagattggg aagaaaagga attaaggaag ttggctgctg aagcagagct
     9001 tcctatttat atcagcaatt tttcaggaga attttcagaa gcgcgtgcac gaaattttcg
     9061 ttatgatttt tttcaagagg tcatgaaaaa gacaggtgcg acagctttag tcactgccca
     9121 ccatgctgat gatcaggtgg aaacgatttt gatgcgcttg attcgaggaa ctcgcttgcg
     9181 ctatctatca ggaattaagg agaagcaagt agtcggagag atagaaatca ttcgtccctt
     9241 cttgcatttt cagaaaaaag actttccatc aatttttcac tttgaagata catcaaatca
     9301 ggagaatcat tattttcgaa atcgtattcg aaattcttac ttaccaaaat tggaaaaaga
     9361 aaatcctcga tttagggatg caatcttagg cattggcaat gaaattttag attatgattt
     9421 ggcaatagct gaattatcta acaatattaa tgtggaagat ttacagcagt tattttctta
     9481 ctctgagtct acacaaagag ttttacttca aacttatctg aatcgttttc cagatttgaa
     9541 tcttacaaaa actcagtttg ctgaagttca gcagatttta aaatctaaaa gccagtatcg
     9601 tcatccgatt aaaaatggct atgaattgat aaaagagtac caacagtttc agatttgtaa
     9661 aatcagtccg caggctgatg aaaaggaaga tgaacttgtg ttacactatc aaaatcaggt
     9721 agcttatcaa ggatatttat tttcctttgg acttccatta gaaggtgaat taattcaaca
     9781 aatacctgtt tcacgtgaaa catccataca cattcgtcat cgaaaaacag gagatgtttt
     9841 gattcaaaat gggcatagaa aaaaactcag acgtttattt attgatttga aaatccctat
     9901 ggaaaagaga aactctgctc ttattattga gcaatttggt gaaattgtct caattttggg
     9961 aattgcgacc aataatttga gtaaaaaaac gaaaaatgat ataatgaaca ctgtacttta
    10021 tatagaaaaa atagataggt aaaaaatgtt agaaaacgat attaaaaaag tcctcgtttc
    10081 acacgatgaa attacagaag cagctaaaaa actaggtgct caattaacta aagactatgc
    10141 aggaaaaaat ccaatcttag ttgggatttt aaaaggatct attcctttta tggctgaatt
    10201 ggtcaaacat attgatacac atattgaaat ggacttcatg atggtttcta gctaccatgg
    10261 tggaacagca agtagtggtg ttatcaatat taaacaagat gtgactcaag atatcaaagg
    10321 aagacatgtt ctatttgtag aagatatcat tgatacaggt caaactttga agaatttgcg
    10381 agatatgttt aaagaaagag aagcagcttc tgttaaaatt gcaaccttgt tggataaacc
    10441 agaaggacgt gttgtagaaa ttgaggtaga ctatacctgc tttactatcc caaatgagtt
    10501 tgtagtaggt tatggtttag actacaaaga aaattatcgt aatcttcctt atattggagt
    10561 attgaaagag gaagtgtatt caaattagaa agaataatct ttaatgaaaa aacaaaataa
    10621 tggtttaatt aaaaatcctt ttctatggtt attatttatc tttttccttg tgacaggatt
    10681 ccagtatttc tattctggga ataactcagg aggaagtcag caaatcaact atactgagtt
    10741 ggtacaagaa attaccgatg gtaatgtaaa agaattaact taccaaccaa atggtagtgt
    10801 tatcgaagtt tctggtgtct ataaaaatcc taaaacaagt aaagaaggaa caggtattca
    10861 gtttttcacg ccatctgtta ctaaggtaga gaaatttacc agcactattc ttcctgcaga
    10921 tactaccgta tcagaattgc aaaaacttgc tactgaccat aaagcagaag taactgttaa
    10981 gcatgaaagt tcaagtggta tatggattaa tctactcgta tccattgtgc catttggaat
    11041 tctattcttc ttcctattct ctatgatggg aaatatggga ggaggcaatg gccgtaatcc
    11101 aatgagtttt ggacgtagta aggctaaagc agcaaataaa gaagatatta aagtaagatt
    11161 ttcagatgtt gctggagctg aggaagaaaa acaagaacta gttgaagttg ttgagttctt
    11221 aaaagatcca aaacgattca caaaacttgg agcccgtatt ccagcaggtg ttcttttgga
    11281 gggacctccg gggacaggta agactttgct tgctaaggca gtcgctggag aagcaggtgt
    11341 tccattcttt agtatctcag gttctgactt tgtagaaatg tttgtcggag ttggagctag
    11401 tcgtgttcgc tctctttttg aggatgccaa aaaagcagca ccagctatca tctttatcga
    11461 tgaaattgat gctgttggac gtcaacgtgg agttggtctc ggcggaggta atgacgaacg
    11521 tgaacaaacc ttgaaccaac ttttgattga gatggatggt tttgagggaa atgaagggat
    11581 tatcgtcatc gctgcgacaa accgttcaga tgtacttgat cctgcccttt tgcgtccagg
    11641 acgttttgat agaaaagtat tggttggccg tcctgatgtt aaaggtcgtg aagcaatctt
    11701 gaaagttcac gctaagaaca agcctttagc agaagatgtt gatttgaaat tagtggctca
    11761 acaaactcca ggctttgttg gtgctgattt agagaatgtc ttgaatgaag cagctttagt
    11821 tgctgctcgt cgcaataaat cgataattga tgcttcagat attgatgaag cagaagatag
    11881 agttattgct ggaccttcta agaaagataa gacagtttca caaaaagaac gagaattggt
    11941 tgcttaccat gaggcaggac ataccattgt tggtctagtc ttgtcgaatg ctcgcgttgt
    12001 ccataaggtt acaattgtac cacgcggccg tgcaggcgga tacatgattg cacttcctaa
    12061 agaggatcaa atgcttctat ctaaagaaga tatgaaagag caattggctg gcttaatggg
    12121 tggacgtgta gctgaagaaa ttatctttaa tgtccaaact acaggagctt caaacgactt
    12181 tgaacaagcg acacaaatgg cacgtgcaat ggttacagag tacggtatga gtgaaaaact
    12241 tggcccagta caatatgaag gaaaccatgc tatgtttggt gcacagaatc ctcaaaaatc
    12301 aatttcagaa caaacagctt atgaaattga tgaagaggtt cgttcattat taaatgaggc
    12361 acgaaataaa gctgctgaaa ttattcagtc aaatcgtgaa actcacaagt taattgcaga
    12421 agcattattg aaatacgaaa cattggatag tacacaaatt aaagctcttt acgaaacagg
    12481 aaagatgcct gaagcagtag aagaggaatc tcatgcacta tcctatgatg aagtaaagtc
    12541 aaaaatgaat gacgaaaaat aaccctgaga gaggctggag cctctctttt ttgtgcagtt
    12601 taggagctaa agggaacaga atggagaaaa tggaacaaat gtgttttcta atctgttaga
    12661 ctgtatctag aaaggggaaa attatgatta aagaattgta tgaagaagtc caagggactg
    12721 tgtataagtg tagaaatgaa tattaccttc atttatggga attatcggat tgggaccaag
    12781 aaggcatgct ctgcttacat gaattgatta gtagagaaga aggactggta gacgatattc
    12841 cacgtttaag gaaatatttc aaaaccaagt ttcgaaatcg aattttagac tatatccgta
    12901 agcaggaaag tcagaagcgt agatacgata aagaacccta tgaagaagtg ggtgagatca
    12961 gtcatcgtat aagtgagggg ggtctctggc tagatgatta ttatctcttt catgaaacac
    13021 taagagatta tagaaacaaa caaagtaaag agaaacaaga agaactagaa cgcgtcttaa
    13081 gcaatgaacg atttcgaggg cgtcaaagag tattaagaga cttacgcatt gtgtttaagg
    13141 agtttactat ccgtacccat tagtaagtca tgcaaaaaaa atgaaaaaaa ttagaaaaag
    13201 tagttgacaa agtttgaaaa ggctgtataa tagtaagagt tgaaaataac aactcaggtc
    13261 cgttggtcaa ggggttaaga caccgccttt tcacggcggt aacacgggtt cgaatcccgt
    13321 acggactatg gtatgttgcg gttggaacac ttgatgaaaa aaagtttaaa aaaacttaaa
    13381 aatcttcaaa aaagtgttga caagtgaaag tggctgtgat atactaatat agttgtcgct
    13441 tgagagaagc aagtgacaaa gacctttgaa aactgaacaa gacgaaccaa tgtgcagggc
    13501 gctacaacat aagttgtagt actgaacaat gaaaaaaaca ataaatctgt cagtgacaga
    13561 aatgagtaag aactcaaact tttaatgaga gtttgatcct ggctcaggac gaacgctggc
    13621 ggcgtgccta atacatgcaa gtagaacgct gaaggaggag cttgcttctc tggatgagtt
    13681 gcgaacgggt gagtaacgcg taggtaacct gcctggtagc gggggataac tattggaaac
    13741 gatagctaat accgcataag agtggatgtt gcatgacatt tgcttaaaag gtgcacttgc
    13801 atcactacca gatggacctg cgttgtatta gctagttggt ggggtaacgg ctcaccaagg
    13861 cgacgataca tagccgacct gagagggtga tcggccacac tgggactgag acacggccca
    13921 gactcctacg ggaggcagca gtagggaatc ttcggcaatg gacggaagtc tgaccgagca
    13981 acgccgcgtg agtgaagaag gttttcggat cgtaaagctc tgttgtaaga gaagaacgag
    14041 tgtgagagtg gaaagttcac actgtgacgg tatcttacca gaaagggacg gctaactacg
    14101 tgccagcagc cgcggtaata cgtaggtccc gagcgttgtc cggatttatt gggcgtaaag
    14161 cgagcgcagg cggttagata agtctgaagt taaaggctgt ggcttaacca tagtaggctt
    14221 tggaaactgt ttaacttgag tgcaagaggg gagagtggaa ttccatgtgt agcggtgaaa
    14281 tgcgtagata tatggaggaa caccggtggc gaaagcggct ctctggcttg taactgacgc
    14341 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgctgtaaa
    14401 cgatgagtgc taggtgttag accctttccg gggtttagtg ccgtagctaa cgcattaagc
    14461 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca
    14521 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgaa
    14581 atccctctga ccgctctaga gatagagttt tccttcggga cagaggtgac aggtggtgca
    14641 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaacccc
    14701 tattgttagt tgccatcatt cagttgggca ctctagcgag actgccggta ataaaccgga
    14761 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta
    14821 caatggctgg tacaacgagt cgcaagccgg tgacggcaag ctaatctctt aaagccagtc
    14881 tcagttcgga ttgtaggctg caactcgcct acatgaagtc ggaatcgcta gtaatcgcgg
    14941 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga
    15001 gagtttgtaa cacccgaagt cggtgaggta accgtaagga gccagccgcc taaggtggga
    15061 tagatgattg gggtgaagtc gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc
    15121 tcctttctaa ggataaggaa ctgcgcattg gtcttgttta gtcttgagag gtcttgtggg
    15181 gccttagctc agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg
    15241 ctaggctcca ttggtgagag atcaccaagt aatgcacatt gaaaattgaa tatctatatc
    15301 aaatagtaac aagaaaataa accgaaaacg ctgtagtatt aataagagtt tatgactgaa
    15361 aggtcagaaa ataaggttaa gttaataagg gcgcacggtg gatgccttgg cactaggagc
    15421 cgacgaagga cgtgacaaac gacgatatgc cttgggtagc tgtaagtaag cgatgatcca
    15481 gggatttccg aatgggggaa cccaacaggt aatacctgtt acccacatct gttaaggatg
    15541 tgaggaggaa gacgcagtga actgaaacat ctaagtagct gcaggaagag aaagcaaaag
    15601 cgattgcctt agtagcggcg agcgaaacgg cagaagggca aaccgaagag tttactcttc
    15661 ggggttgtag gactgcaatg tggactcaaa gattatagaa gaatgatttg ggaagatcag
    15721 ccaaagagag taatagcctc gtatttaaaa tagtctttgt acctagcagt atcctgagta
    15781 cggcgggaca cgtgaaatcc cgtcggaatc tgggaggacc atctcccaac cctaaatact
    15841 ccctagtgac cgatagtgaa ccagtaccgt gagggaaagg tgaaaagcac cccgggaggg
    15901 gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt cgagcccgtt aatgggtgag
    15961 agcgtgcctt ttgtagaatg aaccggcgag ttacgttatg atgcgaggtt aagttgaaga
    16021 gacggagccg tagggaaacc gagtctgaat agggcgcctt agtatcatga cgtagacccg
    16081 aaaccatgtg acctacccat gagcaggttg aaggtgcggt aagacgcact ggaggaccga
    16141 accagggcac gttgaaaagt gcttggatga cttgtgggta gcggagaaat tccaaacgaa
    16201 cttggagata gctggttctc tccgaaatag ctttagggct agcgtcgaca ttagagattc
    16261 ttggaggtag agcactgttt gggtgagggg tccatcccgg attaccaatc tcagataaac
    16321 tccgaatgcc aatgaattat ggtcggcagt cagactgcga gtgctaagat ccgtagtcga
    16381 aagggaaaca gcccagacca ccagctaagg tcccaaaata attgttaagt ggaaaaggat
    16441 gtggggttgc acagacaact aggatgttag cttagaagca gctattcatt caaagagtgc
    16501 gtaatagctc actagtcgag tgaccctgcg ccgaaaatgt accggggcta aaacaattta
    16561 ccgaagctgt ggataccttt ataggtatgg taggagagcg ttctatgtgt gatgaaggta
    16621 taccgtgagg agtgctggaa cgcatagaag tgagaatgcc ggtatgagta gcgaaagaca
    16681 ggtgagaatc ctgtccaccg taagactaag gtttccaggg gaaggctcgt ccgccctggg
    16741 ttagtcggga cctaaggaga gaccgaaagg tgtatccgat ggacaacagg ttgatattcc
    16801 tgtactagag tatgtagtga tggagggacg cagtaggcta actaaagcag acgattggaa
    16861 gagtctgtct aagcagtgag gtgtgaattg agtcaaatgc ttaattctat aacattgagc
    16921 tgtgatgggg agcgaagttt agtagcgaag ttagtgacgt cacactgcca agaaaagctt
    16981 ctagcgttta aacatactct acccgtaccg caaaccgaca caggtagtcg aggcgagtag
    17041 cctcaggtga gcgagagaac tctcgttaag gaactcggca aaatgacccc gtaacttcgg
    17101 gagaaggggt gctgacttaa agtcagccgc agtgaatagg cccaagcaac tgtttatcaa
    17161 aaacacagct ctctgctaaa tcgtaagatg atgtataggg ggtgacgcct gcccggtgct
    17221 ggaaggttaa gaggagtgct tagcgtaagc gaaggtatga attgaagccc cagtaaacgg
    17281 cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacccg
    17341 cacgaaaggc gtaatgattt gggcactgtc tcaacgagag actcggtgaa attttagtac
    17401 ctgtgaagat gcaggttacc cgcgacagga cggaaagacc ccatggagct ttactgcagt
    17461 ttgatattga gtgtctgtac cacatgtaca ggataggtag gagtctaaga gatcgggacg
    17521 ccagtttcga aggagacgct gttgggatac tacccttgtg ttatggccac tctaacccag
    17581 ataggtgatc cctatcggag acagtgtctg acgggcagtt tgactggggc ggtcgcctcc
    17641 taaaaggtaa cggaggcgcc caaaggttcc ctcagaatgg ttggaaatca ttcgcagagt
    17701 gtaaaggtat aagggagctt gactgcgaga gctacaactc gagcagggac gaaagtcggg
    17761 cttagtgatc cggtggttcc gtatggaagg gccatcgctc aacggataaa agctaccctg
    17821 gggataacag gcttatctcc cccaagagtt cacatcgacg gggaggtttg gcacctcgat
    17881 gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg gttgggctgt tcgcccatta
    17941 aagcggcacg cgagctgggt tcagaacgtc gtgagacagt tcggtcccta tccgtcgcgg
    18001 gcgtaggaaa tttgagagga tctgctccta gtacgagagg accagagtgg acttaccgct
    18061 ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct atgtagggaa gggataaacg
    18121 ctgaaagcat ctaagtgtga aacccacctc aagatgagat ttcccatgat tatatatcag
    18181 taagagccct gagagatgat caggtagata ggttagaagt ggaagtgtgg cgacacatgt
    18241 agcggactaa tactaatagc tcgaggactt atccaaagta actgagaata tgaaagcgaa
    18301 cggttttctt gaattgaata gatattcaat tttgagtagg tattactcag agttaagtga
    18361 cgatagccta ggagatacac ctgtacccat accgaacaca gaagttaagc cctagaacgc
    18421 cggaagtagt tgggggttgc cccctgtg
//
LOCUS       DAVKJU010000041        18379 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVKJU010000041 DAVKJU010000000
VERSION     DAVKJU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18379
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.041"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            253..549
                     /locus_tag="VW191_001346"
     CDS             253..549
                     /locus_tag="VW191_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU9624103.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGVSIE"
     gene            670..1614
                     /gene="manA"
                     /locus_tag="VW191_001347"
     CDS             670..1614
                     /gene="manA"
                     /locus_tag="VW191_001347"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEU9624104.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(1717..3102)
                     /locus_tag="VW191_001348"
     CDS             complement(1717..3102)
                     /locus_tag="VW191_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEU9624105.1"
                     /translation="MVFLTDLRKHNRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALGAQATFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIERSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALRQSFFALSLGVTAMLT
                     YASYLDKKTNLVQSRISIVAMNISVSIMAGLAIFQARSPFNIQSEGGPSLVFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKAFFDAMDFLVSNLLMPFGALFLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQV"
     gene            3185..3930
                     /locus_tag="VW191_001349"
                     /pseudo
     CDS             3185..3930
                     /locus_tag="VW191_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(3959..4414)
                     /locus_tag="VW191_001350"
     CDS             complement(3959..4414)
                     /locus_tag="VW191_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9624106.1"
                     /translation="MNRREAVEFVNMCMIKNGDKILVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4434..5609)
                     /locus_tag="VW191_001351"
     CDS             complement(4434..5609)
                     /locus_tag="VW191_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU9624107.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5668..6513)
                     /locus_tag="VW191_001352"
     CDS             complement(5668..6513)
                     /locus_tag="VW191_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9624108.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6638..7195
                     /locus_tag="VW191_001353"
     CDS             6638..7195
                     /locus_tag="VW191_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU9624109.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7214..7681
                     /locus_tag="VW191_001354"
     CDS             7214..7681
                     /locus_tag="VW191_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEU9624110.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            7770..8399
                     /gene="upp"
                     /locus_tag="VW191_001355"
     CDS             7770..8399
                     /gene="upp"
                     /locus_tag="VW191_001355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEU9624111.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8573..9163
                     /gene="clpP"
                     /locus_tag="VW191_001356"
     CDS             8573..9163
                     /gene="clpP"
                     /locus_tag="VW191_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEU9624112.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9242..9490
                     /locus_tag="VW191_001357"
     CDS             9242..9490
                     /locus_tag="VW191_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEU9624113.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9591..10751
                     /locus_tag="VW191_001358"
     CDS             9591..10751
                     /locus_tag="VW191_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9624114.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVIDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11019..11888
                     /locus_tag="VW191_001359"
     CDS             11019..11888
                     /locus_tag="VW191_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9624115.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAVLGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            11892..12848
                     /locus_tag="VW191_001360"
     CDS             11892..12848
                     /locus_tag="VW191_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEU9624116.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            12848..13612
                     /locus_tag="VW191_001361"
     CDS             12848..13612
                     /locus_tag="VW191_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624117.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13612..14322
                     /locus_tag="VW191_001362"
     CDS             13612..14322
                     /locus_tag="VW191_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624118.1"
                     /translation="MSVLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14736..15392
                     /locus_tag="VW191_001363"
     CDS             14736..15392
                     /locus_tag="VW191_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU9624119.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIVRTSAKVL
                     "
     gene            15500..16595
                     /gene="prfB"
                     /locus_tag="VW191_001364"
     CDS             join(15500..15571,15573..16595)
                     /gene="prfB"
                     /locus_tag="VW191_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU9624120.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16613..17305
                     /gene="ftsE"
                     /locus_tag="VW191_001365"
     CDS             16613..17305
                     /gene="ftsE"
                     /locus_tag="VW191_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU9624121.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17298..18224
                     /locus_tag="VW191_001366"
     CDS             17298..18224
                     /locus_tag="VW191_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9624122.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANAPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5649 a         3442 c         3994 g         5294 t
ORIGIN      
        1 tgaaactaga atagtacacc tctgcttcta aaacattgtt agaaatcaat ttgactgtcc
       61 tgattgattt gtcctattct tgtttcattt tactatagaa tgatttgaag gcatttatga
      121 tatttagctg tacgagagtc tttaaaaagt gttttgatgg tttggatttc ttctttagtt
      181 gatttcatat cactattata taatgctttt ttattttagt atagtattat cgtagaaaat
      241 ggactcggtg ctatggatac accggatgag aatggttatg tagcagatga ctatcggatt
      301 acttacttag aggcccacat caaggccatg cgagatgcca tttacaaaga cggggttgac
      361 ttgcttggtt atacgacatg gggctgtatc gattcggttt cagctggcac aggggaaatg
      421 aataagcgct atggctttat ctatgtagat cgagataatg taggaaacgg agctctcaaa
      481 cgtagcaaga agaagtcctt ctattggtac aaggatgtca ttgatagcaa tggtgtaagc
      541 atcgagtaga gcacatttgt ttactatttt tataaaatct tctccctgct ttataagatg
      601 taaaaaagag ttcaaatgga actctttttt tgctataatg aggggagaaa aatcagacag
      661 gagattaaga tgtcagaacc attattttta caatcagtta tgcaagaaaa aatctggggt
      721 ggtaccaagc tacgtgatga gtttggctac gacatcccaa gtgaaaaagt cggagagtac
      781 tgggctatct cagctcatct aaatggtgtt tctaaagttg ccaatggtcg ttacgaggga
      841 acagatcttg ctactttgta tgcggaacac cgtgaattat ttggcaatcg tccagaacct
      901 gtatttccac ttttgaccaa gatcctcgat gccaacgact ggctcagtgt ccaagttcac
      961 ccagacgatg cttatggact cgagcatgaa ggcgaacttg gaaaaacaga atgttggtac
     1021 atcattgcag cagatgaagg ttcagagatt atctacggtc acaatgccaa gtcaaaagaa
     1081 gaactccgcc agcaaatcga ggataagaac tgggatgcgc tgttgacaaa agtgccagtt
     1141 aaagcaggag atttcttcta tgtaccaagc ggtaccatgc acgctatcgg ggcgggtatc
     1201 ttgattcttg aaacccagca gtctagcgat accacttacc gtgtctatga ctttgaccgt
     1261 aaggatgaca agggcaattt acgtgaactt caccttgaaa aatctatcga tgtcttgaac
     1321 attggtgaac ccgcaaatag ccgtccagtg acaatcaaag cagatgatct acgttccact
     1381 ctccttgtat ccaatgattt cttcgcagtt tacaaatggg aaattactgg aaaagttaac
     1441 tttgaaaaga cagctgacta cagcttgttg agtgtcttgg ctggtcaagg tcaactaact
     1501 gttgacggga aaaactatcc aatccaaaaa gggagacact ttatcctacc aagtgatgtt
     1561 gaatcttgga ctctagaagg gcaagattta gaattaattg ttagtcatcc ataaaaaaga
     1621 aaggacctga gttcattact caagtccttt tgattacata aattgggcga tgaagaccac
     1681 gatgatgatt ggaatgacga aacgaagaag gaagagtcag acttggaaca gtccttgttt
     1741 ccatgctctt tcatcgagat ggagttcctc cattgcaaga gcctttttaa agatatagcc
     1801 tgtaaaaagt gaaaggaaga gagctccaaa tggcatgagg agattggaaa ccaagaagtc
     1861 catagcgtca aagaaggctt taccaaaaat gtgaacatcc gccatgacac cgtaagatag
     1921 ggctgaagga atgccaaaga caaaggtcaa aattcctaaa ataacactcc atttagcacg
     1981 cttgctgttg tcctgattgg tgatattgtc tacattgatt tccagcataa cgacagaaga
     2041 agtgacagtc gcaaagagga agaggacata gaaaatggtt ccaaaaggca tcttgtcaaa
     2101 gagttgaggc aagacgataa agaccaagct cggtccccct tcagactgga tattgaaggg
     2161 ggaccgagct tggaaaatgg ctagacctgc catgatggat accgagatat tcatggctac
     2221 gatggagatt cttgactgga ccagattggt tttcttgtcc aagtaagaag cataggtcaa
     2281 catggctgta acccctagtg agagggcaaa gaaagattgt ctcagagcat agaggagacc
     2341 agcgctagtc agttttgaaa aatctggttt gaggaagtaa agaacccctt ccatggcatt
     2401 tggcaaactg agagagcgtt cgatgatgac gacaaagatg ataaagagca ggggcatcat
     2461 gactttcgaa gctctttcaa tccctttttg aaccccacgt gatacaatga agatattcaa
     2521 taggataaag gtcgcttgag ctcctagagc aatggctgga tttgaaatga ttgaagtaaa
     2581 taactgagca taatcacccg ttccaccaag ttggaacaat ttcccaaact caatacctag
     2641 atagactaga atccatcctc cgataacact gtaaaaagat aagaggataa agagggcaaa
     2701 ggcgccaatc caaccgataa agttgtactt gttattcttg cccagttttc caaaggtttt
     2761 gatagcggaa acgccagcac tacggccaag ggcaaactca gccagcagga gagggaaacc
     2821 gattaaaata gtggaaatga gaaagattag taaaaagcct ccaccgccat tagcagcagt
     2881 catgtaggga aacttccaaa cggaaccaag cccgatgacc gagccagcag atgctagaat
     2941 aaagccaagt ttcgaccccc attgtgattt ttctgacata gttaaactct gatctccttt
     3001 ttcaaaataa gaaaaagcta cttgagttgt caaatagcct tctgtttgat tgatagactt
     3061 gactatccta ttatgttttc taaggtctgt caagaaaacc attttcaagt tttcacacct
     3121 ttctcaaaaa cgcttgactc tgacctaagg ggaggggtta tactatcatt gtaaggagga
     3181 aatcatgtac catataaaag aagctgcgca gctttcaggt gtctctgtca agaccctgcc
     3241 ctgcatcact atgacaagat aggactcttg gtccccttaa agtcggaaaa cggctatcga
     3301 acctatagtc aagaggattt ggaacgcctt caggtcattc tttactacaa atatctaggc
     3361 ttttctttag agaaaatagc agagctgtta aaggaagaaa ggacagattt attgccccat
     3421 ttaactaggc agttggacta tctaactcgc gaaaggcaac atctggatac cttgatttcc
     3481 accttgcaaa aaactattca agaacaaaaa ggagaaagaa aaatgaccat tgaggaaaaa
     3541 ttcacgggat ttagctatca agacaatcaa aaataccacc aagaagcggt agataaatat
     3601 ggtcaagaag tcatgggaca agcgctcgaa cgccaaaaag gtcacgaaga cgaggctacg
     3661 gccgccttta accaagtctt tcaaactttg gcacaaaatc ttcaagttgg tttacctgca
     3721 acagcaaccg aaaaccaaga gcaagcagcc aagctcttgc aagctattcg cacttatgga
     3781 tttgactgct ctattgaggt attcggtcat atcggtaaag gttacgtcta caacccagag
     3841 tttaaggaaa atattgacaa gtttggttct gaaacagccc agtacacgtc agatgccatt
     3901 gcggcttacg ttcagacaaa tgcagaataa ataggctagg aatttcctag cctatttttt
     3961 atttcaaatc ataaagccag tcgtcaccgt ttttgtagta aaagaattca ctgagatctt
     4021 cttctagaaa cacacgaagc atatcagaca tatcatcggt tgcaagtttt agatgagaaa
     4081 gattttcaaa gtcctcccac caaactttcc cttcgtctga agactggagt tcaccagtaa
     4141 agtgttctgt cttgtaaaaa aggacgacat aacgataatc cttgtcgtca taccagtttt
     4201 tgataccaca gagttggggt ttggaaatga tcagaccagt ttcttctttc acttcacgaa
     4261 tgacagcatc gacaaaggat tcgccacgtt caacatgacc accaggaaaa gtaatgccag
     4321 gccagtcggg actaactcgg tcttggacca ggatcttatc tccgttttta atcatacaca
     4381 tgttaacaaa ttcgactgcc tctcttctgt tcattcttca caacctttaa tctttaatca
     4441 taatgcagac ttcccgccac ccagccggta cagagggcag aagtgatgtt aaagccaccc
     4501 gtgtgggcat tgatatccat aacttcgcct gcaaagtgga ggccaggtac cagcttactt
     4561 tcaagggttt taggattgat ttccttgaga ctgactccac ccttggtaac aaaggacttt
     4621 gcaagggaca tttttccagt tacaggaatt ttaagttctt taatggactg gacaagttgt
     4681 tctcgttcct tttcagtcag ctgtttgact ttttcaggat atccttgtac aaaaaattcg
     4741 gccaagcgtt ctggtaacaa ggtttttaaa gcgtttttca aggatttttc ccgattttct
     4801 tctagaaatg taaccaagtc cttctcagaa agttgaggca aaacatcgag tgagagaacc
     4861 tccccacctt tgacaaagct agacatgcgt agggcagcag gacctgacaa accaaagtgg
     4921 gtaaagagta aatcatgagt gatgacatgc ttaccataac ttagggtcac atcgtccaga
     4981 gaaatccctt gtaaggcttt atgtggaaaa tctgttaata aaggactttc agcagcctca
     5041 agatcggtga tggtatgctt aaaatggcga gcaatctcgt gaccaaaacc agtcgaacca
     5101 gtcgaaggat aagacttacc acctgttgtg acaatgagtt tctcacaagt gaaggtttga
     5161 tccgctgact taaggacaaa ctggtcatct acttttttaa cagaaacgat ttctatttga
     5221 gtagcaactt gaccacctag ttcagtgatt ttcttttcca aagcttcgat aatagtccga
     5281 gacttgtcac tggctggaaa gacgcgtccg tggtcttcga ccttaagttt aacaccattt
     5341 tctgtaaaaa aattgatgat gtcatgatta tcgaactggg agaaaacact gtaaagaaag
     5401 cgtccgtttc caggaattcc agctagcagg ttgtctaagc taccattgtt ggtcacattg
     5461 caacgtcccc caccagtccc agctaatttt tttccaagtt tccgattttt ttcgatgagg
     5521 agggttttct gtccataaaa gctactggaa atcgtagcca tcataccagc aggtccccca
     5581 ccgatgacaa tagtatcaaa atgtttcata gctctattgt accacaaaaa aacaagagat
     5641 gatggtcacc tcttgtcaag aatgcaatta atcaatttca tagcccatca gcaaaccgcc
     5701 ctcttctgca tagaaactgc agagaccaga ggttggtaga attttaatat ccgcttgtgg
     5761 gaaggtttca cggattcgct ctgagagctg ttgacaacat ttttcgttat tgcgttgggc
     5821 catgacaata cggccaccag catatccagc ttttactaac tcatcatagg cagcttgaac
     5881 tgatttcttt gatccccttg ctttttgtag caattcgaga gtcccagttt cactagcttt
     5941 tccgaccata cgaatgttga gaaggccaac gaccgtaccg ataagcttgc tcaaacggcc
     6001 gttcttcacc aagttatcga ctttggctag gacaaagagc aacttagttt tttcttgata
     6061 ggcggtgata gcttcaacca cttcttcaaa agacaagccc tggtcaatca agtcattcaa
     6121 tttttctacg agtaggtcaa cttcaccacc agcagataaa ctatcaatca catgaatctt
     6181 agtgtcagga tggtcttcca gataaatatt ctttgctagt tgagcactat tgtgactgcc
     6241 agaaagggta cctgtgatgg ttactaggaa aatgtttttg gcaccttcaa atgctcgcaa
     6301 atagtcatct gggcttggac aagccgattt tgaagcttct gcagttgcat acatggtttc
     6361 catcatttgg tcaatatcga gactggcgtc atcaacaaag acctgatcag ctacttgaat
     6421 ggttaagggg acacttacaa aggttgtgtt aatagctggt gttggcagtt gacgataatc
     6481 acaaccagag tcagcaataa tcttccaagt catagaaatt ctccatcttt gtcagttata
     6541 cattgacaaa ggttctgtct ttttttacaa ttatatcatg aaaacccttg aaacaaaagc
     6601 atcatccgtc tgttgtgaca agtagaaaga aaatgttatg tctgaacgta gaatctctga
     6661 aaagtctctt gaaaatctca gaaaatcaaa ccaagaatcc aatttattaa ccagagaagc
     6721 cattgaaaca gccctcttgc aactcttgga aaaaaaggaa ctgaccaaga ttagtatttc
     6781 tgaattggtc aaacgtgcag gtgtttcgcg tgcggccttt tatcgcaatt atgactccaa
     6841 agaggaaatt ttagagagcg tctttaaacg aactgtccac aatattatgg aacagctgca
     6901 tcattacgat ttaaagacag acctttattt ggtttgggtt caccttttcc gggaggccag
     6961 aaaggaagct agagtaattc aattggcctt ggattaccat ctggaaaaaa tctttgtcca
     7021 agccatgcag gaatttctag aaaaatacca tgggaaatca aaaggtgtca gctcttatct
     7081 tcattccttc tggagctcag ccatcgtctc tgtccttcta aaatggatca aggatggcat
     7141 gaaggtacca gctgaaaaga ttgcagattt aggtttacca ttttttaaaa aatagagaaa
     7201 aaggagaaaa gagatgactg aaaaaagact ggcctgggat gagtattttg cagcccaagc
     7261 tctactaatt gcgaatcgtt ccacttgtaa acgtgctaaa gtgggcgcga ttctggtaaa
     7321 agataataag gttatttcca ctggttacaa tggttcggtg tcagggactg agcattgtat
     7381 tgatcacgaa tgtttggtca ttgaaggcca ctgcgttcgc acccttcacg ctgaggtcaa
     7441 tgctatcctt caaggtgctg agcgtggtgt tcccaaaggc tttacagcct atgtaaccca
     7501 ttttccttgt ctcaactgta caaaacaatt gcttcaggtc ggctgcaagc gcgtggttta
     7561 tatcaaccag taccgaatgg acgactacgc ccaatacctt tatcaagaaa aagggacaga
     7621 attgacccat ttaccacttg agacagtaca gacagctctt aaagaggctg atctaatgta
     7681 aaaattatca aaaataaatg gtttagaaag atttttaaac cgttttttgg tataataaga
     7741 agaataaatt gaaagaagga attccaaaaa tgggaaaaat tgaagttatt aatcacccac
     7801 tgattcaaca caaattgtca atcttgcgtc gtacagatac ttctacaaaa gcttttcgtg
     7861 agctagtaga tgagattgca atgttgatgg ggtatgaagt acttcgtgat cttccactag
     7921 aagatgtgga aatcgaaaca ccaattacaa aaacagttca aaaacaattg gcaggtaaga
     7981 aattggccat cgtcccaatc ttgcgtgcag gtatcgggat ggttgatggt ctcttgagct
     8041 tggttccagc tgctaaagtt ggccacatcg gtatgtaccg tgatgaagaa acacttcaac
     8101 cagttgaata cttggtgaaa ttgcctgagg acattgacca acgtcaaatt tttgtagtag
     8161 acccaatgtt ggcaacaggt ggctcagcaa tcttggctgt tgattctctt aaaaaacgtg
     8221 gcgcatcaaa tatcaaattt gtctgccttg tatctgctcc agagggtgta aaagcccttc
     8281 aagaagctca tccagatgta gaaatcttta cagcagcctt ggatgaacgt ttgaacgaac
     8341 acggttatat cgttccaggt cttggagatg ctggagaccg cttgttcggt acaaaataag
     8401 atcgaaaaga agaatgactt ggaaaagtat ttccagtcac gaaaggaggt tgggtttttg
     8461 tttctgtcta atgaaagcag agcaaaaatt tgaccttttt tgaccaagat attataatag
     8521 tcttatcttc agtcatttga ccaacaaaat taaaactcaa aaggagaaat gaatgattcc
     8581 tgtagttatt gaacaaacaa gccgtggaga acgttcttac gatatttact cacgtcttct
     8641 caaagaccgc atcattatgc tgacaggtcc ggttgaagac aatatggcta actctgttat
     8701 tgcccaactg cttttcttgg atgcccaaga tagtacaaaa gatatttacc tttatgtcaa
     8761 tacaccaggt ggttctgttt cagctggttt ggcaatcgta gataccatga actttatcaa
     8821 ggcagatgtc caaaccattg ttatgggaat ggctgcatct atgggaacag tcatcgcatc
     8881 aagtggagca aaaggcaaac gtttcatgct tccaaatgct gaatacatga ttcaccaacc
     8941 aatgggcggt acaggtggtg gtacccaaca aactgatatg gctatcgctg cagaacactt
     9001 gctcaaaact cgtaatacct tggaaaaaat cttggctgaa aattcaggtc agtcaatgga
     9061 aaaagtccat gcagatgcag aacgtgataa ctggatgagc gcccaggaaa cacttgaata
     9121 tggctttatt gatgaaatta tggccaacaa ttcattgaac taatgatgat agaaggcaaa
     9181 ctcgactggg tttgcttttt ttggtataat agggagagat ttcttagaaa gaggatttat
     9241 catgtttgaa aaagtcaatc gctctggctt gattatctat ctttactata atcgtgatgc
     9301 caaaaaactg caggattatg gagatattac ctatcattcc aagaaacatc gttacttaca
     9361 actctatgtt ccaactcaag aagtggagca attggtcgga cgcttgagca aggaaaaatt
     9421 tattaaaaaa gttagggttt gtcatatcca agagttggaa acaccctttg tgggcaatct
     9481 ttatcgatag gaaaacgtta tcatcgaaaa agttcaagga aaacattgac aattttctga
     9541 taattcggta tattcttaac agactattta agaaataagg agacaaaaag atgaagaaaa
     9601 aatttgccct atcgtttgtg gcgcttgcaa gtgtagcact tcttgcagcc tgtggagaag
     9661 tgaagtctgg agcagtcaac actgctggta actcagtaga ggaaaagaca attaaaatcg
     9721 ggtttaactt tgaagaatca ggttctttag ctgcatacgg aacagctgaa caaaaaggtg
     9781 cccaattggc tgttgatgaa atcaatgccg caggtggtat cgatggaaaa caaatcgaag
     9841 taatcgataa agataataag tctgaaacag ctgaggctgc ttcagttaca actaaccttg
     9901 taacccaatc taaagtatca gcagtcgtag gacctgcgac atctggtgcg actgcagctg
     9961 cggtagcgaa cgctacaaaa gcaggtgttc cattgatctc accaagtgcg actcaagatg
    10021 gattgactaa aggtcaagat tacctcttta ttggaacttt ccaagatagc ttccaaggaa
    10081 aaattatctc aaactatgtt tctgaaaaat taaatgctaa gaaagttgtt ctttacactg
    10141 acaatgccag tgactatgct aaagggattg ccaaatcttt ccgcgagtca tacaagggtg
    10201 aaatcgttgc agatgaaact ttcgtagcag gtgacacaga cttccaagca gcccttacaa
    10261 aaatgaaagg gaaagacttt gatgctatcg ttgttcctgg ttactataat gaggctggta
    10321 agattgtaaa ccaagcgcgt ggcatgggaa ttgacaaacc aatcgttggt ggtgatggat
    10381 tcaacggtga ggagtttgta caacaagcaa ctgctgaaaa agcatcaaac atctacttta
    10441 tctcaggctt ttcaactact gtagaagttt cagctaaagc taaagccttc cttgacgctt
    10501 accgtgctaa gtacaatgaa gagccttcaa catttgcagc cttggcttat gattcagttc
    10561 accttgtagc aaacgcagca aaaggtgcta aaaattcagg tgaaatcaag aataaccttg
    10621 ctaaaacaaa agattttgaa ggtgtaactg gtcaaacaag cttcgatgca gaccacaaca
    10681 cagtcaaaac tgcttacatg atgaccatga acaatggtaa agttgaagca gcagaagttg
    10741 taaaaccata atagaaaaat gttgaaatag ggaatgagcc tttgactcac tccctgtttc
    10801 gatatttaat actcttcgaa aatctcttca aactgcgtca acgtcgcctt ggattatata
    10861 tgtgactgac ttcgtcagtc ttatctacaa cctcaaagca gtgctttgag caacctgcgg
    10921 ctagtttcct agtttgctct ttgattttca ttgagtataa gaacctatca aaaagtgagg
    10981 gaaaaccctc ggaattataa atagaaagag tgaatcttat gctccaacaa ctcgtaaatg
    11041 gtttgattct aggtagtgtt tacgcgctgt tagccctagg atataccatg gtttacggaa
    11101 ttatcaagct catcaacttc gcccatggtg atatttatat gatgggagcc tttatcggtt
    11161 atttcttgat caattctttc caaatgaatt tctttgtagc gcttattgta gctatgctag
    11221 cgacagctat tcttggtgtc gtgattgagt ttcttgctta ccgacctttg cgccactcta
    11281 ctcgtattgc tgttttgatt acggctattg gggtttcttt cctattggag tatggaatgg
    11341 tctatctggt tggtgccaat acccgtgcct tccctcaagc gattcaaaca gttcgatatg
    11401 atttgggacc aattagctta acaaatgtgc agttaatgat tttgggcatt tccttgattt
    11461 tgatgatttt gttacaagtc attgtccaaa agactaagat ggggaaagcc atgcgtgcag
    11521 tatcagtaga tagcgacgcg gcgcaattga tggggatcaa tgtaaaccgt acgattagct
    11581 ttaccttcgc tttgggttct gctcttgcgg gtgcggctgg tgttctgatt gctctttatt
    11641 ataactctct tgagcctttg atgggggtta ctccaggtct taaatctttc gttgccgcag
    11701 tacttggtgg tatcggaatt attcctggtg cggttcttgg tggctttgtg attggtctat
    11761 tggaaacctt tgcgacagcc tttgggatgt cagatttccg tgatgccatt gtttatggaa
    11821 tcttgttgtt gatcttgatt gtccgcccag ctggtatcct tggtaagaat gtgaaagaga
    11881 aggtgtaaac gatgaaggaa aatttaaaag ttaatattct atggttactc cttttgttag
    11941 ctggctatag cttgattagt gtactggttt cagtcggagt acttaatcta ttctatgtac
    12001 agattttaca acaaattgga attaatatta ttttggctgt tggtctcaac ttaatcgttg
    12061 gtttttcagg acaattttca cttggtcatg ctggtttcat ggcgattggt gcctatgcag
    12121 cagctattat tggttctaaa tcaccaacct acggtgcctt ctttggagct atgcttgtag
    12181 gggctttgct ttcaggagca gttgccttac ttgtcggcat tccaaccttg cgcttgaagg
    12241 gggactatct tgcggtagca actctgggtg tttctgaaat tatccgtatc tttatcatca
    12301 atggtggaag tcttacaaat ggtgcggcag gtatcttagg gattcctaac tttacaactt
    12361 ggcaaatggt ttacttcttt gttgtgatta caaccattgc aaccttgaac ttcttgcgta
    12421 gcccaattgg ccgttcaacc ctctctgttc gtgaagatga aatcgctgct gagtcagttg
    12481 gggttaatac gactaaaatt aaaatcatcg cttttgtctt tggtgccatt actgcaagta
    12541 ttgctgggtc acttcaggca ggatttatcg ggtctgttgt accgaaagat tacaccttca
    12601 tcaactcaat caacgttttg attattgttg tatttggtgg actcggttcc attacaggtg
    12661 cgattgtttc ggctattgtt ctgggaattt tgaatatgct tctccaagat gttgctagtg
    12721 tgcgtatgat tatttacgct ttggccttgg tattggtaat gattttcaga ccaggtggac
    12781 tccttggaac gtgggaactg agcctatcac gtttctttaa aaaatctaag aaggaggaac
    12841 aaaactaatg gcattacttg aagtaaaaca gttaaccaaa cattttggtg gtctaacagc
    12901 tgttggagat gtgactcttg aattgaacga aggggaactg gttggattaa tcggtccaaa
    12961 cggagctggg aaaaccaccc ttttcaacct tttgaccggt gtttatgaac caagcgaggg
    13021 aacagtaacc ctagatggtc accttttgaa tgggaaatca ccttataaga ttgcctcttt
    13081 gggacttgga cgtactttcc aaaatatccg tctctttaaa gatttaacag ttttagacaa
    13141 tgttttgatt gcttttggaa accatcacaa acaacatgtt tttactagtt tcttacgctt
    13201 accagctttt tacaagagtg aaaaagaatt aaaggctaaa gctttggaat tgttgaaaat
    13261 ctttgattta gatggtgatg cagagactct tgctaaaaat ctttcctacg gacaacaacg
    13321 tcgtttggaa attgttcgtg cccttgctac ggaacctaaa attctcttct tagatgaacc
    13381 agcagcaggt atgaacccac aggaaacagc cgaattgact gagttaattc gtcgtatcaa
    13441 agatgaattt aagattacaa tcatgttgat tgaacacgat atgaatctgg tcatggaagt
    13501 aacagaacgt atctacgtac ttgaatatgg ccgtttaatt gctcaaggaa ctccagacga
    13561 aattaagacc aataaacgcg ttatcgaagc ttatctagga ggtgaagcct aatgtctgtg
    13621 ttaaaagttg aaaatctttc tgtgcattac ggtatgatcc aagcagtccg tgatgtaagc
    13681 tttgaagtta atgaaggaga agttgtttcc cttatcggtg ccaacggtgc aggtaagaca
    13741 actattcttc gcaccttgtc aggtttggtt cgaccaagtt caggaaagat tgaattttta
    13801 ggtcaagaaa tccaaaaaat gccagctcag aaaattgtgg caggtggtct ttcacaagtt
    13861 ccagaaggac gccacgtctt tcctggcttg actgttatgg aaaatcttga aatgggagct
    13921 ttcttaaaga aaaatcgtga agaaaatcaa gctaacttga agaaggtttt ctcacgcttt
    13981 cctcgtcttg aagaacgtaa gaaccaagat gcagctactc tttcaggagg ggaacaacaa
    14041 atgcttgcca tgggacgcgc tcttatgtca acaccaaaac ttcttctttt agatgaacca
    14101 tcaatgggac ttgccccaat cttcatccaa gagatttttg atatcattca agatattcag
    14161 aagcaaggaa caaccgtcct cttgattgaa caaaatgcca ataaagcact tgcaatctct
    14221 gaccgaggat atgtactgga aacagggaaa atcgtcctat caggaacagg aaaagaactc
    14281 gcctcatcag aagaagtcag aaaagcatat ctaggtggct aaaacaatcc agtggattgt
    14341 tttagtcggc agatgaagat tacgaagtaa tcatcattat agtctgggag actagtttag
    14401 gttgcagata aagattgcga agcaatcatc aaatccagag gattgtttta gccggcagat
    14461 ggagattacg aagtaatcat caatatagtc ctggggacct ttttagtcgg tggatagaga
    14521 ttgcaaacaa atatgcatct acattgaaag cttaatttct aataattgaa aaaatcgaat
    14581 gaaaaatttc ttaccttcat tcacagagct ctatttcaga gctctttttg ctagcttatt
    14641 catacttttt tgaatttcga aaaaggaatg taagcgtttg atagatttac aaaaagattg
    14701 tataatgggg ataagaatag aaaaggagaa gtctcatggc agttaaagat tttatgaccc
    14761 gcaaggtagt ttatattagt ccagatacaa cagtatctca tgcagcagat ttgatgagag
    14821 agcaagaatt gcatcgcttg cctgttatcg aaaatgatca attggttggt ttggtgactg
    14881 agggaaccat tgcacaagca agtccatcta aagcaacaag tctttctatc tatgagatga
    14941 attatcttct gaataagaca aaagtaaaag atgtcatgat tcgcgatgtt gtcactgtct
    15001 caggctatgc tagtctagaa gatgcaactt atctgatgtt gaaaaataag attggtattc
    15061 tccctgtcgt agataaccat caagtatacg gagttattac tgaccgtgac gttttccaag
    15121 cctttcttga aattgcaggt tatggcgaag aagggattcg tgtacgcttt gttacagaag
    15181 atgaagttgg tgttcttgga aaaattgttt ctttgattgt agaagaaaat ttgaatatct
    15241 cccatacagt caatattccg cgtaaggatg gtaaggtgat tatcgaagtg caaatcgatg
    15301 gatcaattga tttaccagcc ttgaaagaaa aatttgaagc aaatggtatt caagtggaag
    15361 aaatcgttcg cacttcagca aaagtcttgt aagaagggaa gcccaaaggc ttcttttttc
    15421 atgaaaaggg gattagagca aaagatggaa agaaatgata aaatatgcta taatgaaata
    15481 atgtaaaaaa ggagtattta tggacatttc agtaattcgt cagaaaattg acgcaaatcg
    15541 tgaaaaatta gcttctttca gggggtctct ttgacctcga agggctagag gaagagattg
    15601 ccatcttgga aaacaagatg acagaacctg atttttggaa cgataatatt gcggcccaaa
    15661 aaatgtcgca agaattaaat gaattaaaaa acacttacaa taccttccat aagatggaag
    15721 agttgcagga tgaagtcgaa attttattgg attttttggc tgaagacgag tcagtgcatg
    15781 atgaactggt agcgcagtta gccgaacttg ataagataat gaccagctac gagatgactc
    15841 tactcttgtc agaaccttat gaccacaaca atgccatctt ggaaatccat ccaggttctg
    15901 gtggtactga ggcgcaggac tggggtgata tgttgcttcg tatgtatact cgttatggta
    15961 atgctaaagg ctttaaagtg gaagtgttgg attaccaagc aggtgatgag gctggtatta
    16021 agtcggtaac tttatcattt gaagggccta atgcctatgg tctcctcaag tcagaaatgg
    16081 gtgttcaccg cttagtgcga atctcaccat ttgactctgc caaacgtcgc catacctctt
    16141 tcacatctgt agaagtgatg ccagaattgg atgatactat tgaagtggaa atccgtgaag
    16201 atgatatcaa gatggatacc ttccgttcag gtggtgccgg tggacaaaac gtcaataagg
    16261 tttcaacagg tgtacgttta acctacattc caactggaat tgttgtccaa tcaacagtgg
    16321 atcgtaccca gtatggaaat agagatcgcg ccatgaagat gttgcaggct aagctctatc
    16381 aaatggagca agagaagaag gctgcggagg tagattctct caaaggtgag aaaaaggaga
    16441 tcacttgggg aagccaaatc cgttcttatg tcttcacgcc ttatactatg gtaaaagatc
    16501 accgaactag ctttgaggtt gctcaggtag ataaggttat ggatggggac ctagatggtt
    16561 ttatcgatgc ttatctcaag tggcgaatta gctaagatag aaaggaactc acatgtcaat
    16621 tattgaaatg agagatgtcg ttaaaaaata cgacaacgga acgactgctc tacgcggtgt
    16681 ttcggttagc gttcaaccgg gggaatttgc ttacatcgta ggaccttcag gagcagggaa
    16741 gtcaactttt attcgttctc tgtatcgtga agtaaaaatc gataaaggaa gtctatcagt
    16801 tgctggtttt aatctggtta agatcaaaaa gaaagatgtc ccgcttctac gtcgtagtgt
    16861 tggggttgtc ttccaagatt ataaattgtt accaaagaaa actgtctatg aaaatattgc
    16921 ttacgctatg gaagtaatcg gggaaaatcg ccgtaatatc aaaagacgag tgatggaagt
    16981 tttggacttg gttggattga agcataaggt tcgttctttc ccaaatgaac tctcaggtgg
    17041 ggagcaacag cggattgcga ttgcgcgtgc aattgtaaat aatcccaaag tattgatagc
    17101 tgatgagcca acaggaaatc tggatccgga taattcatgg gaaattatga atctcttgga
    17161 acggattaac ctacaaggaa caactatttt gatggcgact cataatagcc agattgtaaa
    17221 taccttgcgc caccgtgtca ttgccattga aaatggccgt gtcgttcgtg acgaatcaaa
    17281 aggagagtat ggatacgatg attagtagat tttttcgcca tttatttgaa gccttaaaaa
    17341 gtttgaaacg aaatggttgg atgacagtag ctgctgtcag ttcagtcatg attactttga
    17401 ccttggtggc aatatttgca tctgttattt tcaatacagc gaaactagct acagatattg
    17461 aaaataatgt ccgtgtagta gtttatatcc gaaaggatgt ggaagataat agtcagacaa
    17521 ttgaaaaaga aggtcaaact gttacaaata atgactacca caaggtatat gattctttga
    17581 agaacatgtc tacggttaaa agtgttacct tttcaagtaa agaagaacaa tatgaaaaat
    17641 taaccgagat aatgggagat aactggaaaa tctttgaagg agatgccaat cctctctatg
    17701 atgcctatat tgtagaggca aacgctccaa atgatgtaaa aactatagcc gaagatgcta
    17761 aaaaaattga aggtgtctct gaggttcaag atggcggtgc caatacagaa agactcttca
    17821 agttagcttc atttatccgt gtttggggac tagggattgc tgctttgtta atttttatcg
    17881 cagttttctt gatttcaaat accattcgta ttaccattat ttcccgcagt cgcgaaattc
    17941 aaatcatgcg cttggtcgga gctaaaaaca gttatatccg tggaccgttc ttgttagaag
    18001 gagcctttat cggtttattg ggagctatcg caccatctgt tttggtcttt attgtttatc
    18061 aaattgttta ccaatctgtc aacaaatcgt tggtagggca aaatctatcc atgattagtc
    18121 cagatttatt tagtccgttg atgattgccc tactatttgt gattggggtt ttcattggtt
    18181 cattgggatc aggaatatcc atgcgccgat tcttgaagat ttaggtaaaa tagctgcttt
    18241 tatgaggaga ttgtaaaatc tccttttttg ctacaagagt ttttgaaaag agatgcgcag
    18301 aagaaaagag cttccaaaga agtcccccag agaagacttc ttgatggcta atttgaactt
    18361 gaaatttggc gcactagaa
//
LOCUS       DAVKJU010000042        17592 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVKJU010000042 DAVKJU010000000
VERSION     DAVKJU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17592
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.042"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..962
                     /locus_tag="VW191_001367"
     CDS             <1..962
                     /locus_tag="VW191_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEU9624123.1"
                     /translation="TNKILLMPDLFNYLLTGKFATEKSIASTTQLFDPRSQNWNQNIL
                     KLFELDSSLLPEIVSEGNVLGRIKKEYGLGDIPVVNVCSHDTASAIVSVPKTEGSLFI
                     SSGTWSLVGVELTSPILTTESFSYGFTNEVGKDGVITFLKNCTGLWIIEELRRSFERR
                     GKAYSFDDIRTMVEKEKENLPLIDTESTEFATESDMHKTLTEYLAYHHETREWTDGQL
                     FKIVYESLAETYRKAIELLEELTHKVYKRIYVIGGGARASYFNQMIADRTGKEVLTGS
                     TEGTAVGNIVVQLLSQGKINEDTELKDIMTNIADTQYYYPQLS"
     gene            991..2283
                     /locus_tag="VW191_001368"
     CDS             991..2283
                     /locus_tag="VW191_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU9624124.1"
                     /translation="MNKKSLLKCAVIGLVATFGLAACGTSKDASGGSSSGKEVLEFYH
                     GYHHSEDEWPVAKTMRDLYDKFAEEHKDSGVEFKPTPVNGDLKDIMNNKVASGEFPDV
                     IDLAGNAVSLAAIEQKLVLDLKPYIDSNKLEKSVGLNYKQNQKDGKIYTVHEQLFTMG
                     LWYNKDIFAKAGAKTPDQWNTWDDFTQAMASIRKQDGVYAFGAGEPSIRLFNTVLGTT
                     ENGRKLLDKPLTKEGIESKEFADALKMVMKEIQANGSKNAGGDANAYSKDFQEGKSAV
                     FFNGVWASGEMSKNPSLAPGIYPAGVAISSSGGGITISSKMSEAKQKLALEFLKYMTS
                     DDVQKVIFEKVGANPSNENVNVKELSEKSSEATTKILGQAITQVKNAKAVVPTVSDVW
                     GGDVHTAIINALTESAAENVDVDQKVKSTQDVLKSLIG"
     gene            2371..3300
                     /locus_tag="VW191_001369"
     CDS             2371..3300
                     /locus_tag="VW191_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9624125.1"
                     /translation="MAQKIMSLQNRKNQKRRFIFLFLLPTLICFFLFYFYSVVTIFLT
                     SFAKWDYTNLNTPEFLGFDKLFENYRYVFKEYPFFTEALINSVRWAVIGVIIQVPLAV
                     SVAITLSKKLKGWKISRNLYIVPSIISSAAMGLIFLQIYNPNYGVVNQIIHLFNPSFK
                     DSVLLTPGLNIVAMTGAYIFFAGASTIMILGQIFAIPEEVQEAAILDNITGWRKEWYI
                     TIPMIKGTIKTVSIMAATSGFLLYNEVFFLTNGAAGTKSISFVIRELAVASSRTQYAR
                     ANTIGVIQILGGMLIIVCINILFRERKRLKGGK"
     gene            3303..4142
                     /locus_tag="VW191_001370"
     CDS             3303..4142
                     /locus_tag="VW191_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9624126.1"
                     /translation="MNTHINGISKKGKVLIYGYMLLTILISIFPIAWIFLSSLKADPM
                     KNPGISLPTDFTLEGYINVFTKLHVFTYFWNSFKVVSISVIISIVMISMSSYVIARME
                     FRGKKLVTSMLYSTLFIPATAMTFPVYRLVNELGIYNTPVALILVYSCSGIAMSFFII
                     KNYFEIIPKELEEAAEIDGATYAQTFWKVMLPIARPGILTAAVLAFINNWNEYYWASM
                     LVIDKNELTVPALLGQFTTSFNTNYNGLFSAIVVIVLPPIILFAFTSKYFIEALGGGA
                     VKG"
     gene            4139..5458
                     /locus_tag="VW191_001371"
     CDS             4139..5458
                     /locus_tag="VW191_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9624127.1"
                     /translation="MKHTLETINSRTQWFREARFGMFIHWGLYSIPGKGEWIRSHQKL
                     SIEDYEPYFRAFDPKEYNPREWAKQAKAAGMKYMVLTAKHHDGFCLFDSKFTDYKATN
                     TPAGRDLVKEFVDAVRAEGLKVGLYFSLIDWHHPDFPKYADLNHPMRGNEAYRDEKIN
                     FDSYLEYLHNQVKEIVTGYGQIDILWFDYSYEDMVGEKWGASKLIDMVRHYQPNVIVD
                     NRLETSGEGFGSIVTDEITSYAGDFVSPEQIVPHEGIRNFKGEPVPWELCLTMNNNWA
                     YNPTDYLYKSSQTLIRKLVECVSKNGNMILNVGPDALGRINDSSKKILDNFHRWMSRN
                     GEAIYGCSGDENLPKPDWGYYTRNGNTVYAHVFEQPIGPLALLGISKENVKRMSFLHD
                     GSEVKISESWTTNAYKGICFAQFGEVPHFTYPLPDLIDSVIKIELRE"
     gene            5464..7335
                     /locus_tag="VW191_001372"
     CDS             5464..7335
                     /locus_tag="VW191_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HEU9624128.1"
                     /translation="MKIENKNVRRNFFWGDGRFYTTDIVNKRAGVMIKNVSKEEFTIT
                     LENGIRLSSNHFSAIVREEGDTRIQVSFVCPSIRLRLIFESRDDVLSKQLVLESSTEV
                     IKSVEVESFEFETEDNIFYPKRQDCIKEMANFSGYYVELGQPVYANSLFLGMEFPMSE
                     NKVDGRHYVSRYYLGTVVNQEKSLWSCIIGGACSYKKEEIQEAFFEYVEGIAQPSYFR
                     KQYNSWYDHMTDITEEGILKSFSEIRDGFENHGVHLDAYVVDDGWTNYQSVWEFNHKF
                     PNGLRNIKHLVNGFGSSLGLWIGPRGGYNGTEIIMSDWLEAHPELNIGSKNLISNDVN
                     VADFNYLNQMKKRILEYQKEFDISYWKIDGWLLQPDKPDKSGPHGMYTMTVVYEFLIQ
                     LLIDLRKERGGKDCWLNLTSYVNPSPWFLQWVNSLWIQISQDVGFTENAGNDINRMIT
                     YRDIQYQEFLEKREIQLPMWSLYNHEPIYAVSANTWYMDHQMFASIPDFEAYLLFIST
                     RGNAFWEFHYSFDMFDEERWKANARAVKWIEENYQTLKYSKKIGGSPEKFEIYGYKCH
                     NQKTSTEILSLRNPAQIKQKIKIENLSIENFTRVIGDFTIQEDEIELAPYSIVILKK"
     gene            7357..9573
                     /locus_tag="VW191_001373"
     CDS             7357..9573
                     /locus_tag="VW191_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEU9624129.1"
                     /translation="MTIYINKDETVFHLAMKDSSYIFRILENGELQHLHFGKRIHVKE
                     NYNQLMAYEKRGFEVSFSEEFEDIQQSMIQNEYSSYGKGDFRHPAFQVQGMNGSRITT
                     LKYQGFELEKGKNRLNSLPSTFDDIGQCAETLTIILTDSILDLTVRLNYTIFPEYNVL
                     VRNTEFLNNSNNKLTLLKAMSLQLDLPDSQYDFIQFSGAWLRERQLYRTSLRPGIQAI
                     DSLRYSSSPQQNPFFMLSRRETTEHSGEVYGFNFIYSGNFQNMIEVDHFDTARVTVGI
                     NPVEFRFLLDPAESFVTPEAIVIYSDQGMNQMSQQLSDFYRHHLVNPNFSQASRPIIL
                     NSWETFYFDLSTEKILDLAKAAKDLGIELFVLDDGWFGHRKDDKSSLGDWVTDRSRLP
                     EGIGFLADEIHKIGLQFGLWFEPEMISIDSDLYKNHADWTIHLLDREKSVGRNQYVLD
                     LTRQEVVDYLFDSISKIIIKTNLDYIKWDMNRHITDIYSIELDSEQQMEFGHRYILGL
                     YQLLDRLITKFPSVLFESCSSGGGRFDLGLMYYAPQAWTSDDTDPIERLKIQHGTSYG
                     YSPSMMTAHVSISPNEQSGRQTSLDTRTNVAYFSSFGYELDVTRLSVEEKEQVREQIQ
                     FYKKYRSLFQYGDFYRINSPFSCDSASWQVVSKDKCQSILLYAQLNSKLNPGYTRVYF
                     SGLDKDKCYSVSRFDEFFYGDELMNAGIKVSLSNLALCVPEYLTKLFVIEEVVCKY"
     gene            9584..12601
                     /locus_tag="VW191_001374"
     CDS             9584..12601
                     /locus_tag="VW191_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HEU9624130.1"
                     /translation="MESKKIAKQILIATAVLTSFLGSNLVYADVVQSNSNNRASTETA
                     RVTGNNLEKLITKDKEIDKEMTYLSDMDWSSATHGDIDKTKTVQKDAPFTTGNKGEHT
                     KISLLTSDDKVKYFDKGIGTVADSPSVISYDISGQGFEKFETYIGIDQSANSSRSDHA
                     VVDRIEIEIDGKVVYSSSVTNPEGFRYNTQAQFISVTIPQNAKKISLKSFAGEHTWGD
                     EVVFADAKLIKTVSTQTITPDLLNKGINGGVYLSDLEWVDATHGDDDKSKTVQKDKPF
                     TPGNNGSNNKIKLLIDGKEVEFNKGLGTVASNPSSIKYDVSGANVTRFISYVGIDRSA
                     NHLNSDYADIQKFEVVADGKVIYSSDSKYPKGIKYDTSAFLVDVEIPKDTQTIELKSY
                     SGKHTWADELVLGGALFMANGKFKNPNDWSEVDKRREINNEHPLLMMPLYANGEEFNQ
                     GKYTFWGGDTLTGKWENIPDDLKPYTVIQLHPDDLPKRDGAARDFYEHMLEEAAKYVN
                     PKTGKNEPIPVILTVYTAGNMPYYTSAHWLSTSWIDKMYQKYPNLHGIFSTENYWIWA
                     NDIENKAADYLKVSAKNGGYFIWAEQNNGSAIEKAFGKNGKIAFQKSVDKYWKNLIFM
                     FKNTPAAEGNDSTTESYMKGLWLSNHTYQWGGLMDTWKWYETGKWKLFASGNIGKSQG
                     DRQWLTEPESMLGEEALGVYLNGGVVYNFEHPAYTYGVNNKESLLFSEVIKEFFRYVI
                     AHPAPSKEKVLEDTKVFIHGDYSNKGNGKFFVNVNTDREQTPLYMTGRYNVIPAIPGV
                     LKTDKLKESVSGSRIQIKEITSPEFSSTQARKEYLNKLYPMNYEGDIFAQKLDNRWFV
                     YNYKVNENVKQTGKLKFNSLEMDVEFEPHTYGIFERISNGLKVNLNNFRTNKDSLWSN
                     AQDANQAKKLPQLTKKGAIKWIEEHYIKDTQFGEKRVTKIVLRGIDKLPTIHSLSGTN
                     NSYDQPSLNFDQKNHMVTITINSNGNLEFELHF"
     gene            12616..13344
                     /locus_tag="VW191_001375"
     CDS             12616..13344
                     /locus_tag="VW191_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU9624131.1"
                     /translation="MSQDEKLIREQICDVCHKMWQLGWVAANDGNVSVRLDEDTILAT
                     PTGISKSFITPEKLVKLNLKGEILEAEGDYCPSSEIKMHIRCYEEREDVRAVVHAHPP
                     IATGFALAHIPLDTYSLIESAIVVGAIPITPFGVPSTMEVPEAITPYLPDHDVMLLEN
                     HGALTVGSDVITAYYRMETLELVAKTTFHGRMLLSTKGIEEQEIARPTLERLFSMREN
                     YKVTGRHPGYRKYNGDGSMKETEK"
     gene            13357..15123
                     /locus_tag="VW191_001376"
     CDS             13357..15123
                     /locus_tag="VW191_001376"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEU9624132.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAATSHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTRHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGVSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            15269..16420
                     /locus_tag="VW191_001377"
     CDS             15269..16420
                     /locus_tag="VW191_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEU9624133.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            16565..17449
                     /locus_tag="VW191_001378"
     CDS             16565..17449
                     /locus_tag="VW191_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9624134.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
BASE COUNT         5861 a         2588 c         3677 g         5466 t
ORIGIN      
        1 agaccaataa gattctttta atgccagatt tgtttaatta tctcttgaca ggtaagtttg
       61 ctacagaaaa aagcattgct tcaacaactc aattatttga tcctaggagt caaaattgga
      121 atcagaatat cttaaaacta tttgaattgg attcatcttt acttcctgaa attgtttcag
      181 agggaaatgt tcttggaagg ataaaaaagg agtatggttt aggcgatatt cctgttgtga
      241 atgtttgtag tcatgataca gcaagtgcga ttgtctcagt acctaagaca gaaggtagtt
      301 tatttatttc atcaggtact tggtctttgg ttggagtgga acttacttca ccgattctta
      361 ctaccgaatc cttcagttat ggatttacaa atgaagtcgg taaagatgga gtgattacat
      421 ttctgaagaa ttgtacaggg ttgtggatca tagaggaact aagacgttca tttgaacgaa
      481 gagggaaagc ctattctttt gatgatatta ggacaatggt ggagaaagaa aaagaaaatc
      541 ttcctctgat tgatactgaa tcaactgaat ttgcaacaga atctgatatg cacaagactt
      601 tgacagaata tctagcttat catcatgaaa ctagagagtg gacagatgga caactattta
      661 agattgttta tgaaagccta gctgaaacgt ataggaaagc gatagagtta ctagaagaac
      721 taactcataa ggtttataag aggatatatg tgattggagg aggtgctaga gccagttact
      781 ttaaccaaat gattgctgat agaactggta aagaggttct tacaggttcg actgagggta
      841 cagctgtggg gaatattgtt gtgcagctat tgagtcaagg taaaataaac gaggatacag
      901 agttaaagga tattatgaca aatattgctg atacacaata ttattatcct cagctatcat
      961 aaacaaaaat aaaaaaacgg aggttttgtc atgaacaaaa aaagtctttt gaaatgcgct
     1021 gttatcgggt tagtagctac ctttggttta gcagcttgtg gaacatctaa ggatgcaagc
     1081 ggtggaagtt cttctggtaa agaagtatta gaattttatc atgggtatca tcatagtgaa
     1141 gatgaatggc ctgtagcgaa gactatgcga gatttatatg ataaatttgc agaagaacat
     1201 aaagatagtg gtgtagaatt taaaccaact cctgtaaatg gtgatttgaa ggatataatg
     1261 aataacaagg ttgctagtgg tgaatttcct gatgtgattg acctagcagg taatgcagta
     1321 tcattagctg cgattgaaca aaaacttgtt ttagatttga aaccatatat cgattcaaat
     1381 aagcttgaaa aaagtgttgg tttgaactat aagcaaaacc aaaaagatgg aaaaatttat
     1441 acagttcacg aacagttatt tacaatgggt ttgtggtaca acaaggacat atttgcaaaa
     1501 gcgggtgcta agacacctga tcagtggaat acttgggatg atttcactca ggcaatggca
     1561 agcattagaa aacaagatgg agtgtatgca tttggtgctg gagaaccatc tattcgcttg
     1621 tttaatacag tattaggaac cacagaaaat ggacgtaaat tattagataa accattaaca
     1681 aaagaaggaa ttgaatctaa agaatttgca gatgctttga aaatggttat gaaagaaatt
     1741 caagctaatg gttctaagaa tgctggtggc gatgccaatg cttattccaa agatttccaa
     1801 gaaggtaaat ctgcagtatt ctttaatggt gtgtgggctt ctggtgagat gtctaagaac
     1861 ccaagtcttg cacctggaat ctatcctgca ggtgtagcaa ttagttcttc tggaggtggt
     1921 atcactatct caagtaagat gtctgaagct aaacaaaaac tagctcttga gtttttgaaa
     1981 tatatgacta gcgatgatgt acaaaaagta atttttgaaa aagtaggagc aaatccttct
     2041 aatgaaaatg taaatgtaaa agaactttca gaaaagagtt cagaagctac tactaaaatt
     2101 ttaggacaag caatcactca agttaagaat gcaaaagcag ttgttccaac tgtaagtgat
     2161 gtttggggtg gcgatgtaca cacagctatt atcaatgcat tgacagaaag tgctgctgaa
     2221 aatgtagatg ttgatcaaaa agtaaaatct actcaagatg tgttgaaatc attgattggt
     2281 taattgtttt agggagtgaa aacgaagctc agttggcact gccaactgag cttgatttta
     2341 cttatatagt tgaaaaaaag gagtttgttt atggcacaaa aaattatgag cctacaaaat
     2401 aggaagaatc aaaaaagacg atttatcttt ttatttcttt tgccaacgtt gatttgtttc
     2461 tttctatttt atttttattc tgtagtaaca atctttttaa cttcatttgc taaatgggat
     2521 tatacaaatt taaacacccc tgagtttctt ggatttgata aattatttga aaattatagg
     2581 tatgttttta aagaataccc attttttaca gaagctctaa ttaattctgt tcgttgggct
     2641 gtaattggtg ttattattca agttccttta gctgtatctg ttgcaattac attatctaag
     2701 aaattaaaag gctggaagat atctaggaat ctttatattg taccaagtat catttctagt
     2761 gctgctatgg gcttgatttt tcttcaaatc tataatccaa actatggtgt tgttaaccaa
     2821 attattcatc tatttaatcc atcgtttaaa gattcagtac tgttgactcc aggattaaat
     2881 atagtagcta tgactggcgc ttatatcttc tttgcaggag catcaaccat tatgattctt
     2941 gggcaaattt ttgctattcc agaagaagtt caagaagctg ctattttaga caatattact
     3001 ggttggagga aagagtggta tattacgatt ccgatgatta aggggacaat taaaactgtt
     3061 tcaattatgg cagcaacttc aggatttttg ctctataacg aagtattctt tttgacaaat
     3121 ggtgctgcag gaacaaaaag tatcagtttt gttattcgag aattagcagt ggctagctca
     3181 cgaactcagt atgctcgtgc aaatacaatt ggagttatac aaatcttagg tggaatgttg
     3241 attatcgttt gtattaatat tttattcaga gaaagaaaaa gactgaaagg tgggaaatga
     3301 ttatgaatac acatataaat ggtattagta aaaaaggcaa agttcttata tatggttata
     3361 tgctccttac cattttaatt tctattttcc ctattgcgtg gattttttta tcatcattaa
     3421 aagcagatcc tatgaaaaat ccaggtatta gtttaccgac tgactttact cttgaaggtt
     3481 atataaatgt ttttacaaaa cttcatgttt ttacttactt ttggaatagc tttaaagtag
     3541 tgtctatatc agttattatt agcattgtta tgatctccat gtcttcatat gttattgcta
     3601 ggatggaatt tagagggaag aagttggtga cttctatgct gtattctact ctatttattc
     3661 cagcaacagc tatgactttt ccagtttata gattagtaaa tgagttgggg atttataata
     3721 ccccagttgc tctaattctg gtctactctt gtagtggaat tgctatgagt ttctttatta
     3781 taaagaacta ttttgaaatt attccaaaag aattggagga ggcagcagag attgatggtg
     3841 cgacatatgc tcaaactttt tggaaagtga tgttgccaat tgctagacca ggaattttaa
     3901 cagcagcagt acttgccttt attaataact ggaatgaata ttattgggca tctatgttgg
     3961 tgattgataa aaatgaatta acagtaccag cattactagg tcaatttact acaagtttta
     4021 atactaatta taatggtcta ttttcagcta ttgttgtaat cgtattacca ccaatcattc
     4081 tattcgcatt tacaagtaaa tacttcattg aagctttagg aggaggagca gtaaaaggat
     4141 gaaacataca cttgaaacta tcaatagtag aactcagtgg tttagagaag caagatttgg
     4201 gatgttcatt cattgggggt tatattctat tccaggaaaa ggggaatgga ttcgtagtca
     4261 tcagaaatta tctattgaag attatgaacc atattttcga gcttttgatc ctaaggaata
     4321 taaccctaga gaatgggcta aacaagcaaa agctgcaggg atgaagtata tggttttaac
     4381 ggctaaacat catgatgggt tttgtttgtt tgattcaaaa tttacagatt ataaagcaac
     4441 aaatacacct gcaggtagag acttagttaa agaatttgta gatgctgttc gagctgaagg
     4501 tttaaaagta ggcttatatt ttagtttgat tgactggcac catcctgatt tccctaaata
     4561 tgctgatcta aatcatccaa tgagaggaaa tgaagcctat cgagatgaga aaattaactt
     4621 tgacagttat ttggagtatt tacacaatca agttaaagaa attgtaactg gttatggtca
     4681 gattgacata ttatggtttg attattctta tgaagacatg gtgggtgaaa aatggggagc
     4741 ttcaaaatta atagacatgg ttcgtcatta tcagccaaac gttatcgtag ataatagatt
     4801 agaaacatca ggtgaaggat tcggaagtat cgttacagat gaaattacct cttacgctgg
     4861 agattttgtt agtccagagc aaattgttcc tcacgaaggt attcgaaact ttaaaggtga
     4921 acctgttcct tgggaacttt gtctaactat gaataacaat tgggcttata atcctacaga
     4981 ttatctatat aagtctagtc aaactttgat aagaaagtta gtggaatgtg ttagtaaaaa
     5041 cggaaatatg attcttaatg taggcccaga tgctttggga agaattaatg attcaagtaa
     5101 gaaaatatta gataattttc atcgatggat gtctcgaaat ggtgaggcaa tttatggatg
     5161 tagtggagat gaaaatttac caaaaccaga ttggggatac tatactcgta atggaaatac
     5221 ggtttatgct catgtatttg aacaacctat aggtccttta gctcttcttg gtattagtaa
     5281 agaaaacgta aaaagaatga gctttcttca tgatggaagt gaggtgaaaa tttcagaaag
     5341 ctggacgacc aatgcctaca aaggaatttg ttttgcacag tttggagaag ttccacattt
     5401 tacctatcca ttaccagact tgattgatag cgtaatcaaa attgagttga gagagtagtt
     5461 tatatgaaaa ttgaaaataa aaatgtaaga cgtaatttct tttggggaga tggtcgattt
     5521 tatactactg atatcgtaaa caaacgggca ggagtaatga taaaaaatgt ttcgaaagaa
     5581 gaatttacca ttacattgga gaatggtata agactttctt caaatcattt ttcagcaatc
     5641 gttagggaag agggagatac gcgaattcag gtttcttttg tttgtccttc cattcgttta
     5701 cgtttaatct ttgaatcgag ggatgatgtt ctttcgaagc aattagtttt agaatcatct
     5761 actgaggtta ttaaatctgt agaggtagag agttttgagt ttgaaacaga agacaatatt
     5821 ttctatccga aaagacaaga ttgtattaag gaaatggcga atttttccgg ttattatgta
     5881 gaattgggac aacctgttta tgcgaattct ttattcttag gaatggaatt tccaatgtct
     5941 gaaaacaagg tagatggtag acactatgta tcaagatatt acttgggaac tgttgtaaat
     6001 caagaaaaga gtttgtggtc ttgtattatt gggggagcat gttcttataa aaaagaagag
     6061 attcaagagg cattttttga atatgttgaa ggaatagctc aacctagtta tttccgtaaa
     6121 cagtataatt cctggtatga ccatatgacc gatattacag aggaaggtat tttaaaaagt
     6181 ttttctgaga ttcgagatgg atttgaaaat catggagttc atttagatgc ttatgttgtt
     6241 gatgatggtt ggacaaacta tcaatcagtt tgggaattca atcataaatt cccaaatggt
     6301 ttgagaaata ttaaacatct tgtaaatgga tttggttcaa gcctaggatt gtggattggt
     6361 ccccgaggtg gttataatgg gacagaaatc attatgagtg attggttaga agcacatcca
     6421 gagttaaata ttggatctaa aaatttgatt tcaaatgatg taaacgtggc tgattttaac
     6481 tatctcaatc aaatgaagaa aaggatattg gaatatcaaa aagaattcga tatcagctat
     6541 tggaaaattg atggctggtt acttcaacct gacaaacctg ataagagtgg accgcacggt
     6601 atgtatacca tgacagtggt ttatgagttc ttaattcaac tgttgataga tctaagaaag
     6661 gagagaggag gaaaagattg ttggttaaac ttgacttctt atgtaaatcc tagtccatgg
     6721 tttttacagt gggtcaatag tttatggatt caaatatctc aagatgtagg ctttacagag
     6781 aatgcaggta atgatatcaa tcgtatgata acttaccgag atattcagta tcaagaattt
     6841 ttggaaaaac gtgagataca gttacctatg tggtcgcttt ataatcatga accaatctat
     6901 gctgtcagtg caaatacctg gtacatggat catcaaatgt ttgcatcaat accagatttt
     6961 gaagcttatc tattatttat ttcaacaaga gggaatgctt tttgggagtt tcactattct
     7021 tttgatatgt ttgatgaaga acgctggaaa gccaatgctc gtgccgtcaa atggatagag
     7081 gagaattatc aaacactaaa atatagtaaa aaaataggag gaagtcctga aaaatttgaa
     7141 atttatggtt ataagtgtca caatcagaag acttctactg aaattctctc tctgagaaat
     7201 cctgctcaga ttaaacaaaa aataaaaata gagaatcttt cgatagagaa ttttaccaga
     7261 gtaattggag attttactat tcaagaagat gagatagaat tggcaccgta ctctatcgtg
     7321 atactgaaga agtaaagaaa aaattgtgag gtagagatga cgatttatat taataaggac
     7381 gagaccgttt ttcatttggc aatgaaagat agtagttata tttttagaat tttagaaaat
     7441 ggggaacttc aacatctaca ttttgggaaa aggattcatg tcaaggaaaa ttataaccaa
     7501 ttgatggcct atgaaaaaag aggatttgaa gtatcttttt ctgaagaatt tgaggatatt
     7561 caacagtcta tgatacaaaa tgaatattct tcatatggga aaggagattt tcggcatcca
     7621 gcctttcaag ttcaaggaat gaatggtagt aggataacga cactaaaata tcaaggtttt
     7681 gaacttgaaa aagggaaaaa tcgtcttaac tctctacctt caacatttga tgatattggt
     7741 cagtgtgcgg aaacattaac gattatttta acagattcca tattagattt aactgttaga
     7801 ctaaattaca caatttttcc ggaatacaat gtcttagtta gaaatacgga atttttaaat
     7861 aatagcaata ataagttgac tcttttgaaa gcaatgagct tacagctaga tctacctgat
     7921 agtcaatatg actttattca attttctgga gcatggctga gggaacgtca gttatataga
     7981 acttcgctta gaccaggtat tcaagcaata gatagcttga gatactcatc aagtcctcag
     8041 caaaatcctt tctttatgct atcaaggagg gaaactacag agcatagtgg tgaggtttat
     8101 ggttttaact ttatctattc tggaaatttt caaaatatga ttgaagttga ccattttgac
     8161 accgctagag taacggtagg aataaatcca gtagaatttc gttttttatt agatcctgcc
     8221 gaaagttttg tgacaccaga agcaattgtg atctattctg atcaagggat gaatcagatg
     8281 agccaacaac tatcagattt ttatcgacat catttagtta atcctaattt ttctcaagct
     8341 agtcgtccta taatactcaa tagttgggaa acattttatt ttgacttgag tacagaaaaa
     8401 attttagatt tagcaaaggc tgctaaagat ttagggatag aattatttgt actggatgat
     8461 ggttggtttg gtcataggaa agatgacaaa agttctctgg gtgattgggt aacagataga
     8521 agtcgccttc ctgaaggtat tggatttctt gcagatgaaa ttcacaaaat aggtttacaa
     8581 tttggtttgt ggtttgagcc tgaaatgatt tctattgata gtgatttgta caagaatcat
     8641 gccgattgga ctatccattt gttagacaga gagaagtcag taggaagaaa tcaatatgtg
     8701 ttggatttga cgagacagga agttgttgat tacctttttg attctatttc taaaatcata
     8761 atcaaaacaa atctggatta tatcaaatgg gatatgaatc gtcatataac agatatttat
     8821 agtattgaac ttgattctga acagcagatg gaatttggtc atcgatatat cttaggtctt
     8881 tatcagttat tagatcgttt aataactaag ttcccttcag ttctatttga atcttgttct
     8941 tcaggtggtg gacgttttga tttaggactt atgtattatg caccgcaagc gtggacgagt
     9001 gatgatacgg acccgataga aagattgaaa attcagcatg gaacttctta tggatattct
     9061 ccatcaatga tgacagccca tgtttctatt tctccaaatg aacaaagtgg aagacaaacg
     9121 agtttggaca ctaggacaaa tgtagcttat tttagttctt tcggttatga attagatgtt
     9181 acgagattgt cggtagaaga aaaagaacaa gtgagagaac aaattcagtt ttataaaaaa
     9241 tatcgttcat tgtttcaata tggggatttc tataggataa acagtccttt tagttgtgat
     9301 tctgctagtt ggcaagttgt ttcaaaagat aaatgccaat cgattttatt gtatgctcaa
     9361 ttgaatagta agttgaatcc aggttataca agagtttatt ttagtggttt agataaagat
     9421 aaatgctatt ccgtctctag atttgatgag ttcttttatg gagatgaatt aatgaatgct
     9481 ggaataaaag tcagtttaag taatttagca ctttgtgttc cagaatatct tacaaaatta
     9541 tttgttattg aagaagttgt atgtaaatat tgaggaaata aaaatggaaa gtaaaaaaat
     9601 agcaaaacag attttaatag ctacggctgt tttaacaagt tttttaggaa gtaatttggt
     9661 ttatgcagat gtagttcaaa gtaattctaa taacagagct agtactgaaa ctgctagagt
     9721 aacagggaat aacttagaaa aacttattac taaagataaa gaaattgata aagagatgac
     9781 atatctttca gatatggatt ggtcttctgc aactcatggt gatatagata aaacaaaaac
     9841 agtacaaaaa gatgctccat ttacaacagg aaataaagga gagcatacta agatttcatt
     9901 gctcacttct gatgataaag ttaaatattt tgataaagga ataggaacag tcgcagattc
     9961 tccatcagta atcagctatg atatcagcgg tcaagggttt gaaaaatttg aaacttatat
    10021 aggaattgat caaagtgcta atagttctag atcagaccat gcagtagtgg atagaataga
    10081 aatagaaatt gatggaaagg ttgtctattc tagtagtgta actaatccag aaggattcag
    10141 atataatacc caggcacaat ttattagtgt gacaattcca caaaacgcta aaaaaatatc
    10201 cttaaaatca ttcgcaggag aacatacatg gggtgatgag gttgtatttg ctgatgctaa
    10261 acttattaaa actgttagta ctcaaactat aactccagat cttcttaata aaggaattaa
    10321 tggaggagta tatctttctg atttagaatg ggttgatgct actcatggtg atgacgataa
    10381 atcaaaaact gttcagaaag ataagccttt tacaccaggt aataacgggt ctaataataa
    10441 aataaaatta ctaattgacg gtaaggaagt cgaatttaat aagggattgg gaactgtagc
    10501 aagcaacccg tctagtataa aatatgatgt ttcgggtgct aatgttacaa gatttatttc
    10561 ctatgtcgga attgatagga gtgcaaatca tttaaattca gattacgcag atattcaaaa
    10621 atttgaagtt gttgcggatg gtaaagtcat ttactcatca gattcaaaat atccaaaagg
    10681 aattaagtat gatacatcgg catttctggt tgatgttgaa attcccaaag atacacagac
    10741 aatagaactt aaatcatatt cagggaaaca tacatgggct gacgaattgg ttttaggtgg
    10801 agccttgttt atggcaaatg gtaaatttaa gaatcctaat gattggtcag aagttgataa
    10861 acgtcgtgaa attaataatg aacatccgtt acttatgatg cctttatatg ctaacggaga
    10921 agagtttaac caagggaaat atactttttg gggaggagat acattaacag gtaagtggga
    10981 gaatatacca gatgacttaa aaccatatac tgttatacag ttacatccag atgatcttcc
    11041 taaaagagat ggagctgcta gagattttta tgaacatatg ttagaagaag cagcgaaata
    11101 tgtaaatcct aagactggaa aaaatgaacc aattccagtt attctgacgg tttacactgc
    11161 tggtaatatg ccttattata cttcggcaca ttggttaagt accagctgga ttgataaaat
    11221 gtatcaaaaa tatccaaatt tgcatggtat tttcagtaca gagaattatt ggatttgggc
    11281 aaatgatatt gaaaataagg cggcagatta cttaaaagta tcagctaaaa atggaggata
    11341 ttttatatgg gctgaacaaa ataatggttc tgcaatagaa aaagcatttg gaaaaaacgg
    11401 taagatagca tttcagaaat ctgttgataa gtactggaag aaccttattt tcatgttcaa
    11461 aaatacacct gctgctgagg gaaatgattc aactacagaa agttatatga agggactttg
    11521 gctttcaaat catacttatc aatggggtgg tttgatggat acttggaagt ggtatgagac
    11581 aggtaaatgg aaactgtttg caagtggtaa tatcggaaaa agtcaaggag atagacaatg
    11641 gttgactgaa ccagagtcta tgctaggaga agaagcttta ggggtttacc ttaatggagg
    11701 tgtagtttat aattttgaac acccagctta tacctatggt gtaaataata aagaaagctt
    11761 actctttagc gaagtaataa aagaattttt tagatatgta atagcgcatc cagcaccatc
    11821 caaagaaaag gtacttgagg atacaaaagt attcatacat ggagactatt caaataaagg
    11881 aaatggtaag ttctttgtaa acgtaaatac tgatagagaa caaactccat tatacatgac
    11941 tggtcgttat aatgttattc cagctattcc aggtgtttta aaaacagata aattgaaaga
    12001 gtctgtttct ggtagccgta ttcagattaa ggaaatcact tctccagaat ttagctctac
    12061 tcaggctaga aaagaatact taaataagct gtatcctatg aattatgaag gagatatctt
    12121 tgctcagaaa ttagataata gatggtttgt ctataattac aaggtaaatg agaatgttaa
    12181 acagactggg aaattaaaat ttaattcttt agaaatggat gttgaatttg aacctcatac
    12241 ctatggtatt tttgagagaa taagtaatgg attgaaagtg aatttaaata attttagaac
    12301 aaataaagat tcactatggt ctaatgctca agatgcaaat caggcaaaga aactaccaca
    12361 attgaccaag aaaggtgcga ttaagtggat tgaagaacat tatatcaaag atactcagtt
    12421 tggagagaaa agggttacaa agatagtatt aagaggtata gataaacttc ctactattca
    12481 ttcattatca ggaacaaata attcctatga tcagccatct ctcaattttg atcagaaaaa
    12541 tcatatggtt actattacca ttaacagtaa cggaaatctt gaattcgaac ttcattttta
    12601 gaaaggagtg agtttatgtc tcaagatgaa aaattaattc gtgaacagat ttgtgatgtt
    12661 tgtcataaga tgtggcaact tggttgggtt gctgctaacg atgggaatgt atctgttcga
    12721 ttagatgagg ataccattct tgcaacacct actggtatca gcaaaagttt tattacacca
    12781 gaaaagctgg tgaagttaaa tcttaaagga gagattttag aagcagaagg tgattactgt
    12841 ccttctagtg aaattaaaat gcacattcgt tgttacgaag aacgtgagga tgtccgtgcg
    12901 gttgttcatg cccatccacc gattgcaaca ggatttgctc ttgcacatat tcctttagat
    12961 acttattcac taattgagag cgcgattgtg gttggggcaa ttcctattac cccatttgga
    13021 gtaccgtcta caatggaagt gccagaagca attacacctt atctgcccga tcatgatgtc
    13081 atgctattag aaaatcatgg agctctgact gtcggaagcg atgtcattac agcatactac
    13141 cgtatggaaa ctttagaatt agtcgcaaag acaaccttcc acggaagaat gttactttct
    13201 acaaagggca ttgaggagca agaaattgct cgtccgactt tagaacgtct attctcaatg
    13261 cgagaaaatt ataaggttac aggtcgtcac ccaggctacc gtaaatataa tggagatggt
    13321 agtatgaaag aaacagaaaa ataagaggaa aatagtatga ttcaacatcc acgtattggt
    13381 attcgtccga ctattgatgg tcgtcgtcaa ggtgtacgcg aatcacttga agtgcaaaca
    13441 atgaacatgg ctaaaagtgt ggcagatttg atttcaagca cattgaaata tccagatggg
    13501 gaacctgtgg aatgcgtgat ttctccatct accattggtc gtgttccaga ggctgcgact
    13561 tcccatgagt tgtttaaaaa gtcaaatgtt tgcgcaacaa ttacagttac accatgctgg
    13621 tgttatggta gtgaaactat ggatatgtct ccagatattc ctcatgctat ttggggattt
    13681 aatgggacag aacgcccagg agctgtctat cttgcagctg tactagcttc acatgctcaa
    13741 aaaggaattc cagcctttgg gatttatggt agagatgttc aggaagctaa tgatacagat
    13801 attccagaag atgtcaaaga aaaactttta cgttatgcgc gtgcagctct tgcaactggc
    13861 ttgatgagag acactgctta cctatcaatg ggtagtgttt cgatggggat tggcggttct
    13921 attgtaaatc cagatttctt ccaagaatac ttaggaatgc gaaatgaatc ggtagatatg
    13981 acggagttca cgcgccgtat ggaccgtggt atttatgacc cagaagagtt tgaacgtgcg
    14041 ctcaaatggg tgaaagaaaa cgtaaaagaa ggattcgacc ataaccgtga agaccttgtt
    14101 ttaagccgtg aagaaaaaga tagacaatgg gaatttgtta ttaagatgtt catgattgga
    14161 cgtgacttaa tggttggtaa cccaagactt gctgaacttg gttttgaaga agaagcagtt
    14221 ggtcaccatg ctttagtagc tggtttccaa ggtcaacgcc agtggacaga ccattttcca
    14281 aatggggact ttatggaaac tttcctcaat actcagtttg actggaatgg tattcgaaaa
    14341 ccatttgtat ttgcgacaga gaatgattca ctaaatggtg tgtctatgct ctttaattat
    14401 ctattaacaa atactccaca aatctttgct gatgtgcgta cttattggag tccagaggct
    14461 gttaaacgtg taacgagaca tactttagag ggtcgtgctg cagctggctt cttacatcta
    14521 atcaactctg gttcttgtac attggatggt acaggtcaag ctactcgaga tggcaaacct
    14581 gttatgaaac ccttctggga gttggaagaa agtgaagtgc aggctatgct tgaaaataca
    14641 gacttcccac cagcaaaccg cgaatacttc cgtggaggag gagtctcaac tcgtttcttg
    14701 acgaaggggg atatgccagt aacaatggta cgtctcaatc ttctaaaagg ggttggtcca
    14761 gtgctacaaa ttgcagaagg ttatacactt gaacttcctg aagatgttca ccatacttta
    14821 gataatcgta cagatccagg atggccaact acttggtttg ctccacgttt gacaggaaaa
    14881 ggtgctttca agtctgtcta tgacgtcatg aataattggg gagctaatca cggagccata
    14941 acatatggac acattggagc agacttgatt accttggctt ctatgttgag aattcctgtc
    15001 aatatgcata atgtacctga ggaagatatc tttagaccta aaaattggtc cttatttgga
    15061 acagaagatc tagaatcagc agactatcgt gcatgtcagt tgttgggacc actgcataaa
    15121 taaaacttgt ttatatagga ggtgaactta cgtccctcct atccttttaa aaagatttgt
    15181 taaacaattc acaaataatt aaaaacgaat acaaaaagta atataatgat gttaaataga
    15241 tagcgcggag gcgcaggagg aaaattatat ggctacattt tatgttccgg cagtcaacct
    15301 tattggaaaa ggtgttgtaa atgaagtggg tccttatatc aaggaacttg gctataaaaa
    15361 ggcacttttg gtgacagata agtacatcga aggcagtgat attttaccta agactttaaa
    15421 accactggat acagaaggaa tcgaatatgt catctttagc gatgtagagc caaaccctac
    15481 ttgtaaaaat gtcacagatg gggtagctgc tttgcaagaa catggctgtg actttatcat
    15541 cagtcttggc gggggttctc cacaggatgc agctagttgt atttctatca tggctacaaa
    15601 tggtggaaaa ccacaggatt atgaagggct tcataagtct gctaaaaaag gcttgccagt
    15661 tgtggctatc aatacaacgg caggaacttc tgcagaaatc actattaact atgtgattac
    15721 tgatgaagaa cgcaaggtta agatggtaat ggttgacaag aatagccttg ctcttatctc
    15781 tgttaatgac ccggaactca tgctttccaa acctaaaggc ctgactgctg ctactggtat
    15841 ggatgctctg actcacgctg ttgaagcttt ggtaacacct ggtgcttatg atgtaaccaa
    15901 gaaactgtct attggtgcta ttgagcttat caaggaatat cttcctcgtg ctgtagaaaa
    15961 tggacatgat attgaagcgc gtgaaggtat ggtcaatgcc atcttccttg gtggtatgag
    16021 ctttaataat gctggtcttg gttatgttca ctcaatggct caccaactcg gtgcagtata
    16081 taatttgcca catggtgttt gctgtgccat gttgctacca gttatagaac gtgaaaatgc
    16141 taaacgtgta ccagaagctt tccgcaatgt tgccaaagcc ttgggacttc atgtagaagg
    16201 taaatcagat caagaatgtg ccgattatgc gattgctgag attgagaagc tttctgagac
    16261 agtaggtatt cctaagaaat tgactgaact tggtattgaa gaaaaagatt tcgactttga
    16321 atacctttct aagaatgcct tgattgatgc ctgcgcacca ggaaatccat ttatgccaac
    16381 cttagaagaa acgattgcct tttataaaga gttattttag caagagatta gacgacaata
    16441 ggacaattca cctctaatta ttgacatttc aggaaaaatc gcagtaaaaa cggcacagcc
    16501 ttctttaaat gtgctataat actagaaaaa tacttgtgga ggttccatta tggcaatatt
    16561 tttcatgatt tttctgattg tttgtgtgct cctattggtg atagtcacac tgagtacagt
    16621 ttatgtggtt cgtcagcagt cggtggcgat tattgaacgc tttgggaaat accaaaaggt
    16681 tgctaatagc ggtattcata ttcgcttgcc ttttgggatt gactcgattg cagcacggat
    16741 tcagttgcgc ttgttgcaaa gtgatattgt ggttgagact aagaccaagg acaatgtgtt
    16801 cgttatgatg aatgtagcga ctcagtaccg tgtcaacgag cagagcgtga cagatgctta
    16861 ctataaactc atacgtccag aatctcagat taaatcttat atcgaagatg ctcttcgctc
    16921 ttctgttcca aaattaacct tggatgaatt gtttgagaaa aaagatgaga ttgcccttga
    16981 ggttcaacac caagtagcag aagaaatgac cacttacggc tacattatcg tgaaaacctt
    17041 gattaccaaa gtcgaaccgg atgcagaagt taagcaatcc atgaatgaaa tcaatgcggc
    17101 gcaacgtaag cgggtcgcag cacaagaatt ggcggaagct gacaagatta aaattgtcac
    17161 tgcagctgaa gccgaagcag aaaaagaccg ccttcatggt gtggggattg cccaacaacg
    17221 taaggcgatt gtggatggat tggcagagtc tatcaccgaa ctcaaggaag ccaatgttgg
    17281 catgacagaa gaacaaatca tgtctatcct cttgactaac cagtatttgg ataccttgaa
    17341 tacctttgcc tctaaaggaa atcaaaccat ctttttacca aatacgccaa atggtgtgga
    17401 tgatatccga acacaaatct tgtcagccct tcgcgctgag aagaaataat agactaatac
    17461 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgc cgtatagatg ttactgactt
    17521 cgtcagttct atctgcaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcta
    17581 ctaccttaaa ac
//
LOCUS       DAVKJU010000043        17515 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVKJU010000043 DAVKJU010000000
VERSION     DAVKJU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17515
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.043"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            108..257
                     /gene="blpO"
                     /locus_tag="VW191_001379"
     CDS             108..257
                     /gene="blpO"
                     /locus_tag="VW191_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEU9624135.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            361..480
                     /locus_tag="VW191_001380"
     CDS             361..480
                     /locus_tag="VW191_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEU9624136.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            778..942
                     /locus_tag="VW191_001381"
     CDS             778..942
                     /locus_tag="VW191_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624137.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKAEKK"
     gene            968..1342
                     /locus_tag="VW191_001382"
     CDS             968..1342
                     /locus_tag="VW191_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9624138.1"
                     /translation="MFLARLFLGQLPLLVSTYLFLSRQFLNFSLVFQFLLVVINLTSI
                     LVTVYLTREMRIRKFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAAESYQQIIAY
                     IGAVLCLLIMLLLIWGLKYYKK"
     gene            1971..2354
                     /locus_tag="VW191_001383"
     CDS             1971..2354
                     /locus_tag="VW191_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEU9624139.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            2406..3095
                     /locus_tag="VW191_001384"
     CDS             2406..3095
                     /locus_tag="VW191_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9624140.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVLVLQISFNVQIAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     FLPSILFALPHFSSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            3137..3385
                     /locus_tag="VW191_001385"
     CDS             3137..3385
                     /locus_tag="VW191_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624141.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKNDV"
     gene            3415..4026
                     /locus_tag="VW191_001386"
     CDS             3415..4026
                     /locus_tag="VW191_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9624142.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            4187..4981
                     /locus_tag="VW191_001387"
     CDS             4187..4981
                     /locus_tag="VW191_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU9624143.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            4978..5613
                     /gene="trmB"
                     /locus_tag="VW191_001388"
     CDS             4978..5613
                     /gene="trmB"
                     /locus_tag="VW191_001388"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU9624144.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            5739..6218
                     /gene="rimP"
                     /locus_tag="VW191_001389"
     CDS             5739..6218
                     /gene="rimP"
                     /locus_tag="VW191_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU9624145.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            6262..7398
                     /gene="nusA"
                     /locus_tag="VW191_001390"
     CDS             6262..7398
                     /gene="nusA"
                     /locus_tag="VW191_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEU9624146.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            7420..7713
                     /locus_tag="VW191_001391"
     CDS             7420..7713
                     /locus_tag="VW191_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEU9624147.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            7706..8005
                     /locus_tag="VW191_001392"
     CDS             7706..8005
                     /locus_tag="VW191_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEU9624148.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            8022..10814
                     /gene="infB"
                     /locus_tag="VW191_001393"
     CDS             8022..10814
                     /gene="infB"
                     /locus_tag="VW191_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU9624149.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            11065..11415
                     /gene="rbfA"
                     /locus_tag="VW191_001394"
     CDS             11065..11415
                     /gene="rbfA"
                     /locus_tag="VW191_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU9624150.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            11694..12473
                     /locus_tag="VW191_001395"
     CDS             11694..12473
                     /locus_tag="VW191_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624151.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRVGGITIAK
                     QVMKTVPNKALTKTLWYLILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            12475..12657
                     /locus_tag="VW191_001396"
     CDS             12475..12657
                     /locus_tag="VW191_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624152.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTDRWLIKPEDKK"
     gene            13074..13307
                     /locus_tag="VW191_001397"
     CDS             13074..13307
                     /locus_tag="VW191_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEU9624153.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            13307..14641
                     /locus_tag="VW191_001398"
     CDS             13307..14641
                     /locus_tag="VW191_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEU9624154.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGISAIEISLM
                     EHELMNSDSGVTFEDVIELCDVHANLFKNAIKDVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKGLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            14651..14905
                     /locus_tag="VW191_001399"
     CDS             14651..14905
                     /locus_tag="VW191_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEU9624155.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            15001..15396
                     /locus_tag="VW191_001400"
     CDS             15001..15396
                     /locus_tag="VW191_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEU9624156.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFN"
     gene            15808..16257
                     /locus_tag="VW191_001401"
     CDS             15808..16257
                     /locus_tag="VW191_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEU9624157.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQTPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVVPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            16244..16801
                     /locus_tag="VW191_001402"
                     /pseudo
     CDS             16244..16801
                     /locus_tag="VW191_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124554.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            16803..>17515
                     /locus_tag="VW191_001403"
     CDS             16803..>17515
                     /locus_tag="VW191_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9624158.1"
                     /translation="MSDVKEEVSSLSEKQLRQIDVEYAELNDSDIIERLAYLEINNNE
                     KRIVISDIEPTKEIMSVSDQIFEIQKNFQKIKNMFELFISDVSDFLSIKNKLESKELE
                     IEEADVNRFMIHLLSSGKLFVDFNENQIKQKYSKDSEEFDCIHGFASYQYDTNFAYRF
                     CHSLRNYSQHTDLPINEVKAVSPDDEAVIIDFYIDLDYLLNSNFKWKKLKGELIKLNQ
                     ETSKIDAIALVKEYFNALT"
BASE COUNT         5340 a         3075 c         3927 g         5173 t
ORIGIN      
        1 tcatattatt tctaatctat gataaattaa tttttctctt tattgcttat gtttttttga
       61 taatttttat attaaacttg ttctgtaact gaaaagtgac atactatatg aatacaaaaa
      121 tgatgtcaca attttctgtt atggataatg aaatgcttgc ttgcgttgaa ggtggagata
      181 ttgattgggg aagaaaaatt agttgtgcag caggggttgc atatggcgca attgatgggt
      241 gtgcaacaac ggtttgacat ttctattggg accatttgct ataggaatag gtgtaactgg
      301 tgctgcaggt ggagctatac ttggaggtgt agcctatgca gcaacatgtt ggtggtaatt
      361 atggatttta aaagttttat tattggttta gtagttggta tatttggtcc ttatatggat
      421 gacttaatta gaaaaatatt ttcaaaatct tccaaaaagg atacggatag tactctctaa
      481 aaaataaatt tctcccctgt atttgctagc ttaatttaca ggggagtttc tttaatattg
      541 tttcgcctgt caaaatgata tgtgatagca ggatgaaaag tctacaaaca agttggtatc
      601 aatacaatat aaagaggaaa gtatgttttt catgcttggt ttttttaatt tttacaattc
      661 aagaggtttt gatgaccatt tatgatttga gtgatccaag gtcaaaataa gtgctatact
      721 agcagtgtaa atgttcttgc tcaactaaga taaatcgcac atttaggagg aaatcttatg
      781 aagaaaaaaa tactgattat tttcgttctg tatctaatca tgtccatctt tctatatccg
      841 cttagggaga gtgcttggta tcagctattt tataccatag cctatgtgat tgcagttatg
      901 atctattttg ctatcaataa aaagaaagca gaaaagaaat gaaaactttt cttgctaaaa
      961 acggaacatc ttccttgcga gattgttctt aggtcagttg cccttgcttg tctctactta
     1021 tctatttcta tctcgtcagt ttttaaattt ttccttagtt tttcaatttc ttttagtggt
     1081 tattaattta acttctattt tggtcactgt ttacctcact agggaaatga ggataagaaa
     1141 gtttgaagat gatgatttgg ttagtcctag aaccaatcaa ctcatgttta tcggcttgac
     1201 aggttttatg tctattatat gtttgtatag aggtatcaca gcagcagaat cctatcaaca
     1261 aataatcgct tatattggcg cggttctctg cttgcttatc atgcttttgc tcatttgggg
     1321 tttgaagtat tataaaaagt aggggctaat gaagtttttg caatttagga ggtttcgatg
     1381 acaattcaag atttggatga aaaattttaa aaaacaatga tatactaaac ttgtcaaagt
     1441 tgcaacgggt gctgctacat tatgttaaaa atcgctcttg attgataagc tggatttgtt
     1501 aggaaaggag taaataatta tggataagaa aaagatagtt tcgactataa tatgtatagt
     1561 atttcttgta gtgtcagtag ataatttttt tagagactta accccgttac tgttcatttt
     1621 aaatattatt ggcctgtctt gcttttcagt gctaacatac attaacataa aagagatact
     1681 cttaaatata agtaaatgat gatttggtta gtcctagaac caatcaactt atgtttatcg
     1741 gcttggcaga ttttatgttt gtcatttgtt tgtatagagg tatttcagaa acagaattct
     1801 atcaacaact aatcgcttat ataggtgtat tctctgcttg tttatcacgc ttctgctcat
     1861 gtggggcttg aagtattata aaaagtagct ttctatcctt ttctgacagg atatttgagt
     1921 agaaggctct attaaattgt tagtgaaata aatgaatgga ggtatttaat atgaaataca
     1981 gattattttt tgttattttc ttgagtagtg tgttggatat tcttttaggg acatttttac
     2041 aaatctctat cgtatctatt gggtggcttg ttctttacag tggtttgttt gaagcaggag
     2101 ttttccttct tgctaataaa ggggtggcgg taaaaatcaa ggaagtagat attcgaaatc
     2161 gctttaaatt tatttttgga aaaaccttat ggtttcaaat tcttttgctc atctttttga
     2221 taatcaaact ttatcttggt ttggatgcga ggttgatttt attctatgga catattttca
     2281 ttgtctttaa tgccttaatg tatctattat ctagtagtca ggttagcctt aaaaaaaaca
     2341 aactgtcttc ttaatcttac agcagtagag cctgacaagg tgaggtcata gtagggagga
     2401 tttctatgaa aaagtatcaa cttctattca aaataagtgc agtcttctct tacttatttt
     2461 tcgtatttgg tctttctcag ctgacgctta tcgtccaaaa ttattggcaa ttttcttccc
     2521 agattggtaa tttcgtctgg attcaaaata tcttgagttt actatttagc ggagtcatga
     2581 tttggattct ggttaagaca ggccatggtt atctctttcg cattccaaga aaaaaatggc
     2641 tttggtattc gattttgaca gtattagtgc tagtgctcca gatctctttt aacgttcaga
     2701 tagctaaaca tgttcagtca actgctgaag gttgggccgt attgatcggt tatagtggga
     2761 ccaactttgc tgagctaggt atctatgtaa ccctgttttt cttgactcca ctaatggaag
     2821 agttgattta taggggatta ctgcaacacg ccttctttaa gcattcgcga tttggccttg
     2881 atttgtttct tccgtccatt ttatttgctc ttcctcattt ttcaagccta cctagtttgt
     2941 tagatatttt cgtctttgca acatttggaa tcatctttgc cggtttgacc cgctatacca
     3001 agagtattta tccatcctat gcggtgcatg tgatcaataa tattgtagcg accttcccgt
     3061 ttttgctcac ttttctacat agggtcttgg ggtaaaaaaa caaaaggctt gcttttcagc
     3121 catagaggag gtcatcatgt ataaacactt atttttccta gattccaaaa ctttagatcg
     3181 gttgacaccc tatattctag tcttggcttc tgacaccatt gcttttaatg tttttgtgct
     3241 aacctttgta tctgcggtgg tttttaattt cctaaattcc atgctagctt taatggctat
     3301 attcataggg gctggctatg tggtcggatt ttggttgcta aaatggtttg ttttggaaag
     3361 gctagaacta aagaatgacg tgtaggaagt ctgtttgttg aggaggataa ttttatggag
     3421 ttttttgata aatttcatgc cttgtgtttt ggatttttag tactaataat tgtcattaca
     3481 gttccttata cgattaacca tggggatttt tttcaaaatg aatctgcatt gattattgta
     3541 agtcttcttg taacctcgct gagtgttgct tatgctagaa agtttgaaat gatttctttt
     3601 gggatgttaa gcaagaaaca acttttgctt ttcattgcaa tctttcttct aagtgtgctt
     3661 gagacgctgg tttatattca tttcttcgct gtttcttctg gctcaggggt ccaacacttg
     3721 gcggaagtca gcagaggaat ttccctgtct ttgattttga ctacctcagt ttttggcccc
     3781 atccaggagg aactcatttt cagaggactt cttcaaggtg cggtttttga caattcttgg
     3841 ttagggcttg tgctaacttc ctctctcttt tctttcatgc atggaccttc taatgtccct
     3901 tcgtttattt tttatctact tgggggcttg ttgctgggcc ttgcttataa aaagagccaa
     3961 aacttatggg tttctactct agtccacatg ttttacaaca gttggccact cctatattat
     4021 ttataaaaat tatgaaaagg taagcagaag acggtgctta cctttttcaa gctataatga
     4081 tacccaaacc actccagagg cttttctttg agaatcgggt tagagtggtt gacgaatagg
     4141 ccaaaaacta gtagaatagt aaggaaactt tatacggagg aaagaaatgg atttgggtga
     4201 taatgagcta acactgactc ccatacctgg gaaaagtggc aaggcttata tgggtagcta
     4261 tcctgatggg aagcgcatct ttgtaaaaat gaacacctct ccaatcctac ctggtctagc
     4321 tagagaacaa attgctccac aattattatg gagtcgtcgt ttggcagatg ggcgtgatat
     4381 gtgtgctcaa gaatggttga caggcaagat attgaccccc tatgatatga atcgtaagca
     4441 aatcgtcaat attttaaccc gtcttcatcg ctcacgtccg ttgatgacac aattgagtcg
     4501 tttgggctat gccatggaaa cacctgtaga tttactacag tcttggcaag aaacggctcc
     4561 agatgctttg cgtaaaaatc attttatcag tgaagtgatg gctgatttac gtcagactat
     4621 tccaggattt agagaggacc atgcgaccat tgtccatgga gatgtacgac atagtaattg
     4681 gattgagaca gatagtggct tgatttattt agtagattgg gattcggttc gcttgaccga
     4741 tcgcatgttt gatgtggccc atatgctctg ccattatatt tcagaacatc agtggaagga
     4801 atggttgacc tactacggtt acaagtacaa tcaaacggta ttaagtaaat tgtattggta
     4861 tggtcaattg tcttatttga gtcagatttc caagtattat atgaaccaag atttagaaaa
     4921 tgtcaatcgg gagattcatg gtttgcgtca tttccgagac aagtatggaa agagaagatg
     4981 agagttagaa atcgtaaagg ggcaacagaa ttactagagg caaatcccca gtatgtggtc
     5041 ctcaatccct tggaagccaa ggcaaaatgg cgggacttgt ttggcaatga taatcccatt
     5101 catgtggaag ttggaagtgg aaagggtgcc tttgtttcag gtatggccaa gcaaaaccct
     5161 gacatcaact atatcgggat tgatattcaa aagtctgttt tgagctacgc tttggacaag
     5221 gtgcttgaag ttggagtgcc taacatcaag ctcttgtggg tagatggttc tgacttgact
     5281 gactactttg aagacggtga gattgatcgc ttgtatctga acttttcaga tccatggccg
     5341 aaaaaacgcc atgaaaagcg tcgtttgacc tacaagacct ttttggacac cttcaaacgt
     5401 atcttgcctg aaaatggaga aattcatttc aagacggata accgtggctt gtttgagtac
     5461 agtttagtga gcttttctca atatggcatg aaactcaatg gtgtctggct agatttgcat
     5521 gccagtgatt ttgaaggcaa tgtcatgaca gaatacgagc aaaaattctc aaacaagggg
     5581 caagttatct accgagttga ggcagaattt taagagatag cctaaaatta ggctgtacaa
     5641 gtgcttttgc tttacataag ttggcaaacg tgctatactg atagtaagaa tatgaaaagt
     5701 gaggcgggga aatatcttcg cctcttgctt atgaggaggt ggacgcaatc gcaacaatcg
     5761 tagaattagt cagagaagtt gtagaacctg tcatagaagc tccttttgaa ctcgtggata
     5821 tcgagtatgg aaagattggc agtgacatga ttctcagtat ttttgtagat aaacccgaag
     5881 gaattacctt gaacgacacg gcagacttaa cagaaatgat cagtcctgtc ctagacacca
     5941 tcaagccaga tcccttccca gaacaatatt tcctagaaat taccagtcca ggcttggaac
     6001 gtcctttgaa aaccaaggat gccgtcgctg gagcggttgg aaaatacatc catgtcgggc
     6061 tctaccaagc catcgataag caaaaggtct ttgaaggaac cttgttggcc ttcgaagagg
     6121 acgagttgac tatggaatat atggacaaga cgcgtaagaa aaccgtccaa attccataca
     6181 gtttagtatc aaaagcacgt ttagcagtta aattatagaa aaagaaagga tagcttttga
     6241 ggattcaaaa gtgaagaaaa catgagtaaa gaaatgctag aggccttccg cattttggaa
     6301 gaagacaagg gaatcaaaaa agaagatatc atcgacgcag tagtagagtc gcttcgttcc
     6361 gcttatcgca gacgctatgg tcagtcagac agcgtagcta ttgacttcaa cgaaaaaaca
     6421 ggtgacttta cagtttatac tgtccgtgaa gttgttgatg aagtatttga tagccgtttg
     6481 gaaatcagct tgaaagatgc tcttgccatt aattcagctt atgaacttgg agacaaaatc
     6541 aagtttgaag aagcaccagc tgagtttggt cgtgtagcag cccaatctgc caaacaaacc
     6601 atcatggaaa aaatgcgcaa gcaaacacgt gccatcactt acaatactta caaagaacat
     6661 gagcaagaaa tcatgtctgg tacagtagaa cgctttgaca accgctttat ctatgtcaac
     6721 cttggtagca tcgaagccca attgtcaaaa caagaccaaa ttcctggaga agtttttgct
     6781 tctcatgatc gtatcgaagt ttatgtttac aaggttgaag acaaccctcg tggtgtgaac
     6841 gtctttgtta gccgtagcca tccagaaatg atcaaacgtt tgatggagca agaaattcca
     6901 gaagtttatg atggaactgt tgaaatcatg agcgtggctc gtgaagcagg tgaccgtacg
     6961 aaggttgctg ttcgtagcca caatccaaac gtggatgcta tcggtacaat cgttggacgt
     7021 ggtggtgcta atatcaagaa gattactagc aaattccacc cagctcgtta cgatgctaaa
     7081 aatgaccgca tggtaccaat cgaagaaaat atcgatgtta tcgagtgggt agcagatcca
     7141 gctgaattta tctacaatgc catcgctcct gctgaggttg accaagttat ctttgatgaa
     7201 aacgacagca aacgtgcctt ggtggttgtt ccagataaca agctttctct tgccattggt
     7261 cgtcgtggac aaaacgtgcg cttggcggct cacttgactg gttaccgtat cgatatcaag
     7321 tctgctagcg aatttgaagc catggaagac gctgcttcag tagagttgga agtagaaaac
     7381 gatactgtag aagaataaaa gctgctagag gagggaaaga tgaaaacaag aaaaatccct
     7441 ttgcgcaagt ctgttgtgtc taacgaagtg attgataagc gtgatttgct ccgcattgtc
     7501 aagaacaagg aaggacaagt ctttattgat cctacgggca aggccaatgg ccgcggcgct
     7561 tatatcaaac tagacaatgc agaagcccta gaggcgaaaa agaagaaggt ctttaaccgc
     7621 agctttagca tggaagtgga agaaagcttt tatgacgagt tgatcgctta tgtggatcac
     7681 aaagtgaaaa gaagagagtt gggacttgaa taagcaaaag ataagtaatc tcttggggct
     7741 tgctcagcga gcagggcgca tcatatcggg tgaagaattg gtggtcaagg ccattcaaga
     7801 cggcaaggcc aagttggtct ttctagctca tgatgctgga cccaatctga ccaagaagat
     7861 tcaagataaa agtcattatt atcaagtaga aattgtaacc gtgttttcaa cactggaatt
     7921 aagcatagca gtcgggaaat cgagaaaggt tttggctgta acagatgctg gatttacaaa
     7981 gaaaatgagg tctcttatgg aatagaagag gaggacatga tttgtctaag aaaagattgt
     8041 acgaaatcgc aaaagaactt ggaaaagaaa gtaaagaagt tgtagcgcgt gcaaaagagt
     8101 tgggcttgga tgtgaaaagc cactcatcaa gtgtggaaga agctgtcgct gcaaaaattg
     8161 ctgccagctt taagcctgca gctgctccga aagtagaagc aaaacctgca gcaccaaaag
     8221 taagtgcaga aaagaaaacc gaaaaatctg agccagctaa accagctgta gctaaggaag
     8281 aggcaaaacc ggctgagcca gttgctccga aaacagaaaa agtagcagcg aaaccgcaaa
     8341 gccgtaattt caaggctgag cgtgaagccc gtgctaaaga gcaggcagaa cgacgcaagc
     8401 aaaataaggg caataaccgt gaccaacaac aaaacggaaa ccgtcagaaa aacgatggcc
     8461 gtaatggtgg aaaacaaggt caaagcaacc gcgacaatcg tcgctttaat gaccaagcta
     8521 agaagcagca aggtcagcaa aaacgtagaa atgagcgccg tcagcaagag gacaaacgtt
     8581 caaatcaagc ggctccacgt attgacttta aagcccgtgc agcagcccta aaagcagagc
     8641 aaaatgcaga gtacgctcgt tcaagtgagg aacgcttcaa gcagtatcag gctgctaaag
     8701 aagccttggc tcaagctaac aaacgcaagg aaccagagga aatctttgaa gaagcggcta
     8761 agttagctga acaagcacag caagttcaag cagtggttga agtcgtccct gagaaaaaag
     8821 aacctgcagt ggatacacgt cgtaaaaaac aagctcgacc agacaaaaat cgtgacgatt
     8881 atgatcatga agaagatggt cctagaaaac aacaaaagaa tcgaagtagt caaaatcaag
     8941 tgagaaatca aaagaatagt aactggaata acaacaaaaa gaacaaaaaa ggcaataaca
     9001 agaacaaccg taatcagact ccaaaacctg ttacggagcg taaattccat gaattgccaa
     9061 cagaatttga atatacagat ggtatgaccg ttgcggaaat cgcaaaacgt atcaaacgtg
     9121 aaccagctga aattgttaag aaacttttca tgatgggtgt catggccaca caaaaccaat
     9181 ccttggatgg ggaaacaatt gaactcctca tggtggatta cggtatcgaa gccaaacaaa
     9241 aggttgaagt ggataatgct gacatcgaac gtttctttgt cgaagatggt tatctcaatg
     9301 aagatgaatt ggttgagcgt ccaccagttg ttactatcat gggacacgtt gaccacggta
     9361 aaacaaccct tttggatact cttcgtaact cacgtgttgc gacaggtgaa gcaggtggta
     9421 ttactcagca tatcggtgcc taccaaatcg tggaaaatgg taagaagatt accttccttg
     9481 atacaccagg acacgcggcc tttacatcaa tgcgtgcgcg tggtgcttct gttaccgata
     9541 ttacgatctt ggtcgtagcg gcagatgacg gggttatgcc tcagactatc gaagccatta
     9601 accactcaaa agcggccaac gttccaatca tcgtagccat taacaagatt gataaaccag
     9661 gtgctaaccc agaacgcgtt atcggtgaat tggcagagca tggagttatg tcaactgctt
     9721 ggggtggaga ttctgaattt gttgaaatct cagctaaatt caaccaaaat atcgaagaat
     9781 tgttggaaac agtccttctt gtagctgaaa tccaagaact caaggcagac ccaacagtgc
     9841 gtgcaatcgg tacggttatc gaagcgcgct tggataaagg aaaaggtgcg gtcgcaaccc
     9901 ttcttgtaca acaaggtacc ttgaatgttc aagacccaat cgttgtcgga aataccttcg
     9961 gtcgtgtccg tgctatgacc aacgaccttg gtcgtcgtgt taaagttgct ggaccatcaa
    10021 caccagtctc tatcacaggt ttgaacgaag caccgatggc gggtgaccac tttgccgttt
    10081 acgaggatga aaaatctgcg cgtgcagcag gtgaagagcg tgccaaacgt gccctaatga
    10141 aacaacgtca agctacccaa cgtgttagcc ttgaaaacct ctttgatacc cttaaagctg
    10201 gggaactcaa atctgttaat gttatcatca aggccgatgt acaaggttct gttgaagccc
    10261 tttctgcctc acttcaaaag attgacgtgg aaggtgtcaa agtgactatc gtccactcag
    10321 cggtcggtgc tatcaacgaa tcagatgtga cccttgccga agcttcaaat gcctttatcg
    10381 ttggtttcaa cgtacgccct acaccacaag ctcgtcaaca agcagaagct gacgatgtgg
    10441 aaatccgtct tcacagcatt atctacaagg ttatcgaaga gatggaagaa gctatgaaag
    10501 ggatgcttga tccagaattt gaagaaaaag ttattggtga agcggttatc cgtgaaacct
    10561 tcaaggtgtc taaagtggga actatcggtg gatttatggt tatcaacggt aaggttgccc
    10621 gtgactctaa agtccgtgtt atccgtgatg gtgtcgttat ctatgatggt gaactcgcaa
    10681 gcttgaaaca ctataaagac gacgttaagg aagttacaaa cggccgtgaa ggtggattga
    10741 tgatcgacgg ctacaatgat attaagatgg atgatgtgat tgaggcgtat gtcatggaag
    10801 aaatcaagag ataagatttt ttgctccttt cttaggtggt gagggacgca agcaaaccga
    10861 tggtttcatt gcttattttt gagcctaggg tctcaaaaat cccctgtgat gggactgata
    10921 aatcagttcc atcactttca ccacggcgaa agaagcagat gacttcaaat tgaacttcgt
    10981 ttcaatttaa actgaaaatc aagaagttta aaatagctag gtctgctggc ctagcttttg
    11041 gttcaaagta gagaaaggaa tatcatggca aatcatttcc gtacagatcg tgtgggcatg
    11101 gaaatcaagc gtgaagtcaa tgagattttg caaaagaaag tccgtgatcc acgtgtccaa
    11161 ggtgtgacca tcacagatgt tcagatgctg ggtgacttgt ctgttgccaa ggtttattac
    11221 accattttga gtaaccttgc ttcggataac caaaaagccc aaatcgggct tgaaaaagca
    11281 actggtacca tcaaacgtga acttggtcgc aatttgaaat tgtacaaaat cccagatttg
    11341 accttcgtca aagacgagtc catcgagtat ggaaacaaga ttgacgagat gctacgcaat
    11401 ctggataaga actaaagaag aggggttgcc cctctttttt ggtggaggaa aataggttga
    11461 atttgaaatg gaaaaatatt cttttaaaat atattgaaac tagaatagta cgcctctact
    11521 tctaaaacat tgttagaaat cgatttgact gtcctgatcg atttgtcctg ttcttgtttc
    11581 attttactat aaaaaagggg ttctgtattt tttaatgtta tctaattaga aaatgctttt
    11641 tttgtaggaa atataatatg ataaggtgta aaaaagaaat aaggagtttg tatatggctg
    11701 aacaagactt agctatgcaa gtattacaac aagtggtaaa actaccagtt gttaaggttg
    11761 atcgttcgaa atttttagtg gataagtttt ccaaagaatt gggtccacag gacattccta
    11821 ccttattgga acaaggtcca acgtctctct tatctcaaga aatcttagat cgagtggcta
    11881 atgcctgtat tagggataat gttttattag cgagtgggac ttctgttttg gcaggattac
    11941 ctggagggct tgctatggca attaccattc cagctgatgt ggctcaattt tatggtttct
    12001 ctctgaaatt ggcccaagaa ttaggttata tttatggtta tgaggatctt tgggcttcac
    12061 gcgatgagtt gagtgaagat gctcaaaata cccttttgct ttatctaggc gtcatgttgg
    12121 gggtgaatgg aactgctgct ttgctacgtg ttggtggtat aacaattgcc aaacaggtaa
    12181 tgaaaacagt acctaataaa gctttaacaa agacgctttg gtatcttatt ttgaaaaaag
    12241 tcttaagaat atttggtgtg aatcttacca agggagggct ggccaaagga atggggaaat
    12301 ttattcctat cttgggtggt atcatttcag gtggtttaac ctttgcaact atgaaaccga
    12361 tgggtgaaag cttgcagaaa gaattgtcca agctagttaa ctatagtgaa gttcaatatc
    12421 aagaagatgt tgaaacaatc cgaaaagagg ctgaaatcat cgaaggagag taatatggga
    12481 ctcataaaaa ctctagctaa aatttatggt aattactttt tgaccgtgca aggtgtaaaa
    12541 gtaatgaaaa cgataaagaa agatgataat gccgttgtcg gtctggggaa actttttatt
    12601 gccgacaagt taatggatac ggatcggtgg ctcattaagc cagaggataa aaaatgaaat
    12661 ttttctgggg acttcttgcc attattttta tcaaaccgat tattgggatt gtgaaattct
    12721 tttggatgat catctctttt gcagtccaat tgctgtttta caagatagtg tttaagatat
    12781 tggattggct ctttaaactt atctagatgg taatccaagt tgcagagaac tagcaggaac
    12841 tccactgcta gttttttatt ctctttccat atggtataat ataagcagta aaatcatttt
    12901 atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgcagtata tatgttactg
    12961 acttcgtcag ttctatccac aacctcaaaa cggtgttttg agcaacctgc ggctagcttc
    13021 ctagtttgct ctttgatttt cattgagtat tagaacatac aatggaggtc gtcatggaca
    13081 atatcatcga tgtgtcaatt cctgttgcag aagtggtgga caagcatcca gaagtcttgg
    13141 aaattctagt ggagttgggt tttaaacccc ttgctaatcc cttaatgcgc aatacagttg
    13201 gtcgtaaagt atcacttaaa cagggttcta agctagcagg aactcctatg gacaagattg
    13261 tacgcacact ggaagcgaat ggctacgaag tgattggatt agactaatgg cagatgaacg
    13321 gattcatgtc ctacgggata ttttattaga attgcataat ggcgcctctc ctgagtcggt
    13381 tcaggagcgt tttgatgcga cctttgcggg tatctcagcc attgagattt cccttatgga
    13441 gcacgagctg atgaactcag actcaggtgt cacctttgaa gatgttatag aactctgtga
    13501 tgtccatgcc aatcttttta aaaatgctat caaagatgtc gaagtttcag atactgagca
    13561 tccaggtcac ccagttcgtg tcttcaaaga agaaaatctg gctctccgtg cggccttgat
    13621 tcgcattcgt agattgttag atacctatga gttgatggaa gacgaggaaa tgcttgcgga
    13681 gatgcgcaag ggcttggtgc gtcagatggg gcttgtgggg caatttgaca tccattacca
    13741 acgtaaggaa gaactcttct ttcctatcat ggagcgctat ggacacgatt cacctcccaa
    13801 agttatgtgg ggagtggatg atcagattag ggaactcttt caaacagctc taacgacagc
    13861 caagtcacta ccagaagtgt caattagcag tgtaaaggaa gcttttgaag cttttgcgac
    13921 agagtttgaa agtatgattt tcaaggaaga gtccatcctc ctcatgattc tccttgagtc
    13981 ttttactcag gatgactggc ttcagattgc ggaggagagc gatgcctatg gctatgccat
    14041 catccgtccg tcagagaaat gggtgccaga acgacagatc tttattgagg aaaagattgc
    14101 agaggagcct gtacagctag atacggcaga aggtcaagtt caacaagtca tagatacgcc
    14161 agaaggccat tttaccatta cctttacccc taaggaaaag gaagctgtgc tggaccgcca
    14221 tagtcaacag gcttttggta atggctatct ttcagtcgag caggccaatc tcatcctcaa
    14281 tcatctccct atggagatta cctttgtcaa taaagaagat attttccagt attacaatga
    14341 caatacgcca gctgatgaga tgattttcaa acggacgccg tcccaagtcg ggcgcaatgt
    14401 cgaactctgc catccgccta agtacttgga caaggtcaaa actatcatga aggggcttcg
    14461 tgagggaagc aaagacaagt atgaaatgtg gttcaagtct gagtcgcgag gtaagtttgt
    14521 ccacatcacc tatgctgcag tacacgatga agacggagaa ttccaaggag tgttggagta
    14581 tgttcaggat atccagccct accgtgagat tgatacggac tattttcgtg gattagaata
    14641 aggagaaaaa atgagttacg aacaagaatt tatgaaggaa tttgaagctt gggtcaatac
    14701 ccaaatcatg attaacgata tggcgcacaa ggaaagtcaa aaagtttacg aagaagacca
    14761 ggacgagcgt gccaaagatg ccatgattcg ctacgagagt cgcttggatg cttatcagtt
    14821 cttgcttggt aagtttgaaa acttcaaagt aggcaaggga ttccatgatt tgccagaagg
    14881 cttgtttggt gagcgaaatt attaaacgag aaagattctt gatttttcac taaaatcttg
    14941 atagaatgtt tatgttaaat ccttgtcaga gcagggattt tttattgaaa ggattttatc
    15001 atgtcaaaga aactcaatcg taaaaaacaa ttacgaaatg gcctccgtcg cgcaggtgcc
    15061 ttttcaagta ctgtgactaa ggttgtagat gagacaaaaa aagtcgtgaa gcgtgcagaa
    15121 cagtcagcaa gcgcagctgg taaggctgtt tctaaaaaag ttgaacaagc agtagaagct
    15181 accaaagagc aagctcaaaa agtagctaat tctgtagaag attttgcagc aaacttgggt
    15241 ggacttccac ttgatcgtgc caaaactttc tatgatgaag gaatcaagtc tgcttcagat
    15301 ttcaaaaact ggactgaaaa agaactcctt gacttgaaag gaatcggccc agctaccatc
    15361 aagaaattga aagaaaatgg catcaagttc aattaatttt tcttgagcct tgcatttccg
    15421 aaaaaatctt gctacaatag agccattaga ggtgttttga atcccacatt ttacagaaag
    15481 tggcggcgct gagaagtcca caaatgtgtc aaaactggtt gctaatgact gaaaaaatga
    15541 aatatttctg tctttttatt ttaaagacta aagatgcggg cttggcccga aattgggtgg
    15601 taccgcggat aaacacattc gtccctgtca tgtagatggc agggcttttc ttttacgtct
    15661 tagtcaaagg gggttgttat gaagcaatct tttaacacaa gtaaactata ttatggtttt
    15721 cctgttttta ttttaggata tcaagattag aattttggct ataacatcac gacctgtagt
    15781 tcctcttata gtctaggaga ttgggttgtg attggagtcg ttgcgagaga gaatgccgca
    15841 gagcagatca aacagtatca aaaatttact gtgaatattt ctgatgaaac ttctatgctt
    15901 gcgatggagc aggctggttt tatcagtcat caggagaaat tggaacgttt gggagtgcat
    15961 tatgaaattt ctgaacgaac tcagactcct attttagacg cctgtccact tgttttagat
    16021 tgtcgggtag ataggattgt tgaggaagac ggtatttgcc acatctttgc caagattctt
    16081 gagcgacttg ttgttccaga actcctggat gaaaagggac attttaaaaa tcaactgttt
    16141 gccccaacct atttcatggg agatggatat cagcgcgttt atcgctatct ggacaagcgt
    16201 gtagatatga agggcagttt catcaaaaaa gcgaggaaga aggatggcaa gaactgagct
    16261 gccagataaa atcgaaacag aacgtctcgt tttacgagtc cgtactgtgg cggatgctga
    16321 ggatatcttt gactatgcta gtttgccaga ggtcgcctat ccagcaggtt ttcctccagt
    16381 caagaccttg gaagatgaga tttattatct ggagcacatt cttccggagc gtaatcaaaa
    16441 ggaaaatctc ccagcaggct acgggattgt cgtcaaagga acggataaga tcgttggctc
    16501 tgtcgatttc aaccatcgcc atgaagatga tgtgctggaa atcggctata ccttacaccc
    16561 agactattgg gggtgaggtt atgtgccaga agctgcgcgt gccttgattg acttagcctt
    16621 taaagatttg ggtcttcaca agattgaact aacttgcttt ggatataacc ttcaaagtaa
    16681 acgagtcgcg gaaaagcttg gctttaccct cgaagctcgc ataagagacc gaaaggatgt
    16741 tcaaggaaac cgctgtgaca gtctgatata tggcttgctg aagagcgagt gggaggaata
    16801 agatgagcga tgtaaaagag gaagtaagct ctctatctga gaagcagtta cgccaaattg
    16861 atgttgagta tgctgaatta aatgatagtg atattatcga gcgattagct tatttagaaa
    16921 ttaacaacaa tgaaaaacgt attgtcatct ctgatattga accaaccaag gaaataatgt
    16981 cagtctcgga ccaaattttt gagattcaaa agaattttca aaaaatcaag aatatgttcg
    17041 aattgtttat ttcagatgtc tcggattttt taagtattaa aaataaactt gagtcaaagg
    17101 aattagaaat tgaagaggca gatgtaaatc gtttcatgat tcatttatta tccagtggaa
    17161 aattgtttgt cgattttaac gaaaaccaaa ttaaacaaaa atattccaaa gatagtgagg
    17221 agtttgattg tatacatggt tttgcaagct atcagtatga taccaatttt gcttatcgat
    17281 tttgccattc tttgagaaat tattcgcaac atactgactt accaataaat gaggtaaaag
    17341 ctgttagccc agatgatgag gctgttataa tagatttcta tattgattta gattatcttt
    17401 taaattccaa ctttaaatgg aaaaaattga aaggggagtt gataaaacta aaccaagaaa
    17461 catctaagat tgacgctata gctctagtga aagaatactt taatgctctt acaga
//
LOCUS       DAVKJU010000044        17176 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVKJU010000044 DAVKJU010000000
VERSION     DAVKJU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.044"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(34..264)
                     /locus_tag="VW191_001404"
     CDS             complement(34..264)
                     /locus_tag="VW191_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624159.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(282..923)
                     /locus_tag="VW191_001405"
     CDS             complement(282..923)
                     /locus_tag="VW191_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9624160.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(935..1669)
                     /locus_tag="VW191_001406"
     CDS             complement(935..1669)
                     /locus_tag="VW191_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEU9624161.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALIP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1672..1869)
                     /locus_tag="VW191_001407"
     CDS             complement(1672..1869)
                     /locus_tag="VW191_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624162.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1869..2075)
                     /locus_tag="VW191_001408"
     CDS             complement(1869..2075)
                     /locus_tag="VW191_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9624163.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2085..2398)
                     /locus_tag="VW191_001409"
                     /pseudo
     CDS             complement(2085..2398)
                     /locus_tag="VW191_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2656..3945)
                     /locus_tag="VW191_001410"
     CDS             complement(2656..3945)
                     /locus_tag="VW191_001410"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU9624164.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQVHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLIFEKQGVALPIENALDVYIA
                     VLGDGANAKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISKNFSEIFEKLGFYTQ"
     gene            complement(4023..5270)
                     /locus_tag="VW191_001411"
     CDS             complement(4023..5270)
                     /locus_tag="VW191_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEU9624165.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAVIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(5461..6312)
                     /gene="rgg"
                     /locus_tag="VW191_001412"
     CDS             complement(5461..6312)
                     /gene="rgg"
                     /locus_tag="VW191_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEU9624166.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            6436..6777
                     /locus_tag="VW191_001413"
     CDS             6436..6777
                     /locus_tag="VW191_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9624167.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            6835..6915
                     /locus_tag="VW191_001414"
                     /pseudo
     CDS             6835..6915
                     /locus_tag="VW191_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(7256..8959)
                     /gene="ilvD"
                     /locus_tag="VW191_001415"
     CDS             complement(7256..8959)
                     /gene="ilvD"
                     /locus_tag="VW191_001415"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU9624168.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9192..10124)
                     /locus_tag="VW191_001416"
     CDS             complement(9192..10124)
                     /locus_tag="VW191_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEU9624169.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(10121..10978)
                     /locus_tag="VW191_001417"
     CDS             complement(10121..10978)
                     /locus_tag="VW191_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU9624170.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(10982..12328)
                     /locus_tag="VW191_001418"
     CDS             complement(10982..12328)
                     /locus_tag="VW191_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9624171.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(12341..12625)
                     /locus_tag="VW191_001419"
     CDS             complement(12341..12625)
                     /locus_tag="VW191_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9624172.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(12629..14659)
                     /locus_tag="VW191_001420"
     CDS             complement(12629..14659)
                     /locus_tag="VW191_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEU9624173.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            14850..15857
                     /locus_tag="VW191_001421"
     CDS             14850..15857
                     /locus_tag="VW191_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU9624174.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGVFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15860..16060
                     /locus_tag="VW191_001422"
     CDS             15860..16060
                     /locus_tag="VW191_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEU9624175.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16212..16394
                     /gene="rpmF"
                     /locus_tag="VW191_001423"
     CDS             16212..16394
                     /gene="rpmF"
                     /locus_tag="VW191_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU9624176.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16410..16559
                     /gene="rpmG"
                     /locus_tag="VW191_001424"
     CDS             16410..16559
                     /gene="rpmG"
                     /locus_tag="VW191_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9624177.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(17039..>17176)
                     /locus_tag="VW191_001425"
     CDS             complement(17039..>17176)
                     /locus_tag="VW191_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624178.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT         5575 a         3598 c         2949 g         5054 t
ORIGIN      
        1 atacaaaaat ccgatgaact tcaccggact cttttatttt gaatttttgc ctgctttacg
       61 cttttcagcg atttcggctg cctttcgagg caagacaatt tccgttatgt aagccgtccc
      121 aaaacgcagt acacctgcaa taggagcaaa gacaactgct agatagttat agaagaaatc
      181 gcctttgaag gcataagcta gcgctccaat gatgaaaaat agaacgactg cctgaatcac
      241 tgctaataaa attactcgtt tcatgtgacc tcctgactct attatagcat gagaatcatc
      301 aaaaagccga ctaaattatt caaagcgtga agagaaatac tgtagaccag accttttctg
      361 ctaacgtaag ccaaacccaa actaaaacca aggctaaaat agacaaaaaa ttgttgcaca
      421 tcacctggaa aatgaatcaa ggcaaataga agactagata ccagaagaaa aatcagggtt
      481 cgtttactat tgtcctgctt aggaaagaga tagcgtgcta acatccctct aaaaacaatc
      541 tcttccgtca aaggagcaaa aataaccaca gcaaagaatg agaaaagtgg ttgagacaag
      601 gtcaagtctg tcgctatttg ctgatttact gaaggatcat ctggcaagaa gaattgaacg
      661 accagagata aaaaccaaac caaggcagga agccaaataa atcggttaaa gccgctcttc
      721 tcaatatgaa caggagcctt ctgataccat ttgtaaatgc cgtacacata tactccagcc
      781 aaggccacat agagtagagt aacagcatag ggtgaagcgc ctaaagcaag cgacgcagtc
      841 gcgagcccct gaataaagcc atagataaat aaaaaggata gaagggctag aagaatccag
      901 ccaaggtttt taagtaattt cataaataac tcctttattt gaaataacgt tttaccatag
      961 gtaactgcat cacattgata taaacatgga tggctcctac aagcaagaaa gctagtaact
     1021 gaatctctcc tgtcaagaaa gaaatgataa taagaaaaat atataaggct ggtaagacat
     1081 attggtgtaa ttggaataaa attcgaaaac tctgttccaa attagcctga cgctcccctt
     1141 catcataaga atttatatag ttcaagacat cctttggtgt agcgaaaaat tccaaatcaa
     1201 actgacgaac aatcgcaatg gttttaaaaa gagatttttg agcgactaag aataccacaa
     1261 agagtaaaaa agaaaggaaa aatgtttgag ggtttgtatg caatataatc acctcactta
     1321 atgaaataaa aatagccaat ggaatcgcta cacctgtaat attaaaagca atggttccaa
     1381 actcaagatt ccgatacatt tgcacataat aggtttcatt cagatcgtca tccatctcct
     1441 cttgatacaa ggaatgaaat tttctgcttt tctttaagaa attgaaagtc aaaaacatac
     1501 taatgaaacc tatcagtaaa caaatagctg atatccatgg tatcaaggct tttacatcta
     1561 aaataatttc gtgggattcg acacgtgcct taaacatccc tacaaacatg cccaagaacc
     1621 cccccaagac aatagacatc aaaaataaca atctacgttt ctttttcata ttcattctcc
     1681 tttttcactt gctagatttt tggatttctt ttcaatccat tcaattactg ggatgagagc
     1741 aaagtagacc caaacaaatt ggtcgctttg atagggatta aaccagctta ggtccatccc
     1801 aatcagtaga aatacactga ctaataaagc tatgaccact acataataaa tcactttata
     1861 cttgttcatc actcgtcctc ctccaaacga aataccgatt cgactgtttc gttgaaaatt
     1921 tgagatattt tcagggcaat gataatggat ggggtgtact catcccgttc tagtaggcta
     1981 atggtctgtc tggaaacccc tgccagtttg gctaggtcgg tttgattgag accatcgcga
     2041 gctcgaagct cttttagacg attttttagt tgcatgttac acacctactc tccgtcaaat
     2101 tcaacggttt ggatatcttc aatacgttgc aacttgaatt tttctttccc ctttttatca
     2161 acactacgta gtttgaccca ttcctcgtca acatccacaa cttcccagtt atctggccca
     2221 atatacactc ccgttataat tggttccttt ccaatcattt cttgtaataa tctcgacatt
     2281 tctgcgtttc ctttctcttt tcgctcaagt tttttgattt tattctctag tttcttgatt
     2341 ttttagaatt attagaataa aagaaaatca taaatagtat aaatactagt acccacatta
     2401 taactccttt ctgcttccta tttcttaact tgaattcatt gtaacatatc tttttctttt
     2461 tgacaagtat agttgtcaaa aagtttatga tttttgtcat tttgcaaaag aaaaaggtca
     2521 ggagtaagtt cctgaccact ttatctatca ttaatactct tctaaaatct cttcaaacca
     2581 cgtcagcttc acctacaacc tcaaaaccat gttttgagca acctgcggct agcttcctag
     2641 tttgctcttt gattttcatt gagtataaaa tcctagtttt tcaaagattt ctgagaaatt
     2701 tttgctgatt gtctcaagtg acacttgcac ttcttctcga gtttgattgt tcttgaccgt
     2761 cacttgtccg ctttcgactt cactctctcc tagagtgatg agggtcttag ccgcaaagac
     2821 atcggctgac ttgaattgag ctttgagttt acggttgagg taatcacgct ctgctttgaa
     2881 accttgttgg cgaagagctt ggactagttc tagggctttg gcatttgctc catcacccaa
     2941 gactgcgata tagacatcta gggcattttc gatagggagg gccacgcctt gtttttcaaa
     3001 gatgagaagc aggcgctcta caccaagccc aaaaccaaat ccagcagttt cagggcctcc
     3061 aaagtaagca accaaaccat cgtagcgacc acccgcacag actgtcaagt cattgccctc
     3121 aatctctgtg ataaactcga aaatggtgtg gttgtagtag tccagaccac gcaccatatt
     3181 ggtatcgatg atgtaatcta ctccaagatt ttccaacatc tgacgcacag catcaaaatg
     3241 aacttggctt tcttcatcaa gaaagtccaa gatagacggc gcattctcta ctgccacctt
     3301 gtcttctttt tccttagagt ccaagacacg aagaggattt tcctccaagc gacgttggct
     3361 atccttagac aaggtctcct tgagcggtgt caaatagtca atcaaggctt ggcggtaggc
     3421 tgcacggctc tcaggatttc caagagtgtt gaggtgcaat ttgacacctt gaataccgat
     3481 ttccttcaaa aaatgggctg ccatagcgat tgtttccaca tcggtagctg gattgctaga
     3541 gccaaaacac tcaacaccaa tctggtggaa ttggcgcaag cgccctgcct gtggacgctc
     3601 ataacggaac ataggtccca tgtagtagaa cttgcttggc ttttgcactt ctggggcgaa
     3661 gagtttattt tccacatagg aacggacaac gggcgcagtt ccttctggac gaagggtaat
     3721 atggcggtca cccttgtcat agaaatcgta catttccttg gttacaatat ctgttgtatc
     3781 tccgacagag cggctgataa cctcgtaatg ctcgaaaata ggcgtacgca cttctgcgta
     3841 gttgtagcgt ttgaaaattt cacgggcaaa gccctcaacg tactgccact tggcagattc
     3901 agcaggtaaa atatcctgcg ttccttttgg tttttgtaat ttcataggga atcctcttta
     3961 aacttaatag tcttatttta ccataaatag agggattaaa acagtgagaa aaaaattagg
     4021 atttagatat catttttgag attaagaatt gttaaaaaaa tagctagcaa ggaaagacca
     4081 acaaatagca tccaagtcaa ctgcatgttc catacggcta ctagtgaaaa acaagctgtt
     4141 cccacaggta tggataaggt aaacaataga tctaaaaaat tactagtacg agctagaacc
     4201 tctggagcta gatttttcat gagcatggca ctaatctttg gttgaacttt accagacaca
     4261 tacagagtaa agaagagaaa tagcaaacca agcacgactt gattgaataa attagccaaa
     4321 ccaactagac taagtcctac ggtcgcccac atcatcaatc taggcaagga ctgcttccca
     4381 aaataatcat tgcccgtaag gctactgatg atgactgcta ctaaaacaca gaattgattg
     4441 ataaatagtg cctctgtata agaaaaattc aagagagaat ggctcaaaaa gaagatatta
     4501 taaattccac ccaaagcgcc acccaaggaa ttaataagca agacagcaaa gagcataaaa
     4561 ccaaagtttt tctgtccact tttaagaaaa acgagacgta aatttcggta aattgttagg
     4621 aactggtctt tgatagaaag cttctcattt tttaagtttt caccatcagc agatgacatt
     4681 gacaggctca atttgctttt tcctaaaaag aggatagtgg ctgatactag gaaaaagcag
     4741 gcattgattc ccgcaacgag agaaaaattg ttgaccgata gagctaagag ccagactccg
     4801 aaagcttgac caccaatagc tgaaatatag gtgatgaact gtgaaaaaga ataagcctcc
     4861 atcagatcat cttcagctac tttttcctta ataagaggca tacgcaggcc acctgcaaaa
     4921 tcactgatga tatcactaat gacattgatc aaacacaggc tagaaaaggc aaagagacta
     4981 gcttgctgaa caactagggc tgctagaaaa aatagaaccg cctgaaacaa accgctatag
     5041 accatccatt tgaccttgtc cctcgtgtaa tctgcccgaa tccctgcaaa aactgtaaag
     5101 aaggtcggaa gaatcatgac aatattcgcc atagcaacag caaaagatgc ttgtgacaag
     5161 gtcgatgcat agacgataaa gaccaggttg aaaatcgaag caccaaaagc attgaagaag
     5221 cgtgataaag tcaaaattct ataagctgga tttctaaaca atagtttcat agataatctc
     5281 cctccttgtt ctaagttcta catcaagaat agctttagta tagcacttat gaatttgcga
     5341 aacgattctt ttgaatatat gcaacaaaca aaatgagcaa aaaaacgata gaacttttgt
     5401 aatgacctga gagagggcaa cagttctatc gcttcaaatt ttttcttggt ttgcagatat
     5461 tcaacattcg gtcgtatttc tatagtattc ggcagattta ttacagccca gcatctcaaa
     5521 aatacggaca gcatcctcca tctttttctg gccttccttg actctgcctt gcttgctatc
     5581 aaggagacct tctgcccaca gataaacaat tcggaaatag gtctcatttt ccttgtagaa
     5641 atgctcttcg ataacacgtt taaaataata ggcattggta aattcttcac actcaatact
     5701 agctaaaaag ccattcaata gtatagtatg aaaaaggttt cgattgccag acatttccat
     5761 tagaaaatca gatttacgta ccatttctcg tacatatcta gtaaaaagag aaacagataa
     5821 aaatggagaa ctgactgaaa ataaattgag ttcatagatt ccccagatct cggtagaaaa
     5881 caaataatca tgaaggactt ttccttcctc tgctgttaac tctacccttt catctatgct
     5941 cttcatataa gacttgatca taatggcatt tagaatatgt ttctgtttgt tgtgagaatg
     6001 ggcatgcttt tgatactccc tgcgatataa gtcctcaaga ggtgctatat tctttggttc
     6061 caagacatct gtaatttctt ttctcaactc agaatctgta tcatactgga aacctctcgc
     6121 cagaaagagg atctcctcca cactggcaga tatattttcc agagcaaata gaaacttttc
     6181 caccgaaagc tcactctgac ctgtttcaaa acgggacaac atagacggcg aaaattgtcc
     6241 tccggttgct tgtctcagtg agatatttct tgactctcgt aattgtctaa agacttttcc
     6301 aatctgctcc atagacttcc ccttgattcc gtattttctt cattttatca tatttttcag
     6361 aaaattcatc aaaaacttgc caaattgtca gaattatgag aaaatagagg atatttatca
     6421 cgtggaggga ctgctatgag agacgatatc aaaatcaatg accgtgcttt ggccttgcaa
     6481 gaccaaatta tcgaaaaact agagaaagtt tttgatacag atgtggaatt ggatgtttac
     6541 aatctaggtc tgatttatga aatcaatctg gatgaaacgg ggctctgcaa gattgtcatg
     6601 accttcaccg atactgcctg tgattgcgcc gaaagcctgc ctattgaaat tgtggcaggt
     6661 ctgaaacaaa tcgagggtat caaagatatc aaggttgaag ttacctggtc gcctgcttgg
     6721 aaaatcacac gaatcagtcg ctatggccgt attgcccttg gactaccacc tcgttaagca
     6781 ggccaatcac ttttaaagat gaaaaaagca agccgaaaat ggcttgcttt ttgatactca
     6841 atgaaaatca aagggcaaac tagaaaacta gccgcaggtt gctcaaaaca ctgttttgag
     6901 gttgtggata gaactgacga agtcagctca aaatactgtt ttgaggttgt ggatagaact
     6961 gacgaagtca gctcaaaata ctgttttgag gttgtagata gaactgacga agtcagctca
     7021 aaatactgtt ttgaggttgt agatagaact gacgaagtca gctcaaaata ctgttttgag
     7081 gttgtagata gaactgacga agtcagctca aaacactgtt ttgaggttgc agatagaact
     7141 gacgaagtca gcccaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac
     7201 catacctacg gcaaggcgac gttgacgtga tttgaagaga ttttcaagta tgagtttatt
     7261 ttttacctga cttgtccata ttccagaagt ctgtcacggc tccgcgtgaa gcagatgata
     7321 cgatatgggc atatttaccg aggacaccac ggctgtaaag tggtggcaag gttgtttctg
     7381 ccttgcgttt ttcaagttct tcttcggata cggccatgga aatttctttg gtatcttggt
     7441 caaccgtaac gatatcgccg gtacggagat aggcaattgg tccaccatcc tgagcttcag
     7501 gagcgatatg tccaacaacc agaccataag taccaccaga gaaacgtccg tccgtcaaga
     7561 gggccacctt atctccctga cctttaccaa caatcattga agaaagtgac agcatctcag
     7621 gcataccagg accaccttta ggtccaacaa aacgaacaac gactacatcg ccatcaatga
     7681 tttcatctgt caggacggcc tgaatcgcat cttcttctga gtcaaagacc ttagctggcc
     7741 caacgtgacg acgcacttta acacctgata ccttggcaac tgcaccgtca ggagcaaggt
     7801 tcccgttcaa gatgataagc ggaccatccg cacgttttgg attttcaagt ggcatgataa
     7861 ctttttggcc tggagtcaag tctgcaaagt cagccaagtt ttcagctaca gtcttaccag
     7921 tacatgtgat gcgatctccg tgaaggaaac catttgccaa caagtacttc ataaccgcag
     7981 ggacaccacc gacttcgtag aggtcttgga agacatactg accagatggt ttcaagtcgg
     8041 ccaagtgagg cacacgttct tgaatcgtat tgaagtcctc aagtgacaag tcaacatttg
     8101 cggcatgggc aatggcgagc aagtgaagag tggcgtttgt agaaccaccg agagccatcg
     8161 ttacagtaat agcatcttca aaggcttcac gagtcaagat atctgatggt ttgagaccaa
     8221 gttccaacat cttaacaaca gcacgtcctg ctgcttcgat atcttctttc ttatcagctg
     8281 attcagctgg gtgagaggat gaccctggca aactcatccc tagaacttcg atagcagttg
     8341 ccatggtatt agcagtatac ataccaccac aaccaccagg gccagggcag gcattacatt
     8401 caagacgttt cacgtcctca gctgtcatgt caccgtggtt ccattttccg ataccttcaa
     8461 agacagaaac caagtcgata tctttaccat caagatttcc cggtgcaata gttccaccat
     8521 aggcgaaaat agctgggata tccatattag caatagcaat catagatcca ggcatgttct
     8581 tgtcacagcc accgatagcg acgaaggcat ccacgttgtg accactcata gccgcctcga
     8641 tggagtccgc gatgatgtca cgagatgtta gagagaaacg cataccaggc gttcccatag
     8701 cgatcccgtc cgctacggta atggttccaa actgtacagg ccaagcgcct gcagatttga
     8761 caccttcttt agccagtttc ccgaaatcat gcaagtgaat gttacatggt gtattttccg
     8821 cccaagtcga aatcactccc acaatcgatg tttcaaagtc cttatctgtc ataccagtcg
     8881 cacgaagcat agcacggtta ggtgatttaa ccatgctgtc ataaatgcta ctgcggtgac
     8941 gtttatctaa ttcagtcatc ttatccctcc catttcagtt tttactatta tagcacaatt
     9001 taaaggcaat gaacagaata aaattcttga attttcagaa aattctatac gcatgtgaaa
     9061 tatttaaaat taaaaacaac aaagcggatt agtgcacttt ctgatgacca gaatatgctt
     9121 tttaatccgc tttctttaaa taacgtactg taatttttac agaaattctt tcagataagt
     9181 gtatttaaca tctatcttgc attataaatt tctagaacct tctcttttat attcgattca
     9241 ctcaaaccat actcattaag aagataatcc attttcccta cttgaccgaa tctttcttga
     9301 acacccatcc gatgaatttt tgttattcca tcatcagaga ataattcaca taaagcactg
     9361 ccaattccac ctatctgatt gtggttttct acagtaaata tagtttttcc acttaacatt
     9421 gtttttatct gttctggtat cggtttgatt ctaaataaat ctatcacacc tactgaataa
     9481 cctaatttag acagttcatc tgcaactcga atacttggag caaccattat gccagaagca
     9541 acgattacaa gatcttcacc atgtcttaac tcaatgtagc ccttagaaaa atcttctcca
     9601 ccttgatata cagcctctgg agcttttcta attgttcgaa tatattttag tccttttaag
     9661 tctaatgtct gattcaatat ttcacgaaat tggatatcat cagttgcttc aaaaatgatt
     9721 gatttaggaa ttaaacgtaa caatccaatt tcttcaaatg gcatatgtgt cccaccattc
     9781 atttctgcag ttactcctgc atctgatcca atcacagtgg catccaattg tgcgtatcca
     9841 agagaaataa ataattgatc aaatactctt cgtgaagcaa aaggaccaaa tgtatgaaga
     9901 taaggtctaa acccctgaat agacaagcct gctgcaaggc cgaccatttc tgcttccata
     9961 atcccaacat tcacataacg gtctccaaag tccttttcaa gattatgagt agccatcgaa
    10021 cttgacagat cggcttctaa gactactata tcagaataac tttgattagc ctctaaaagg
    10081 aagtctctat atacatgccg taattctttc gtacttctca tcattctgtt tcctccaatt
    10141 cctgacttaa tctttctaca actgaagtta acatttgtct ctcctctaca gtagggcgaa
    10201 gatgatgatt ggatttcatt tcttccagct cttgaacccc ttgaccttta atagtatcta
    10261 atacaatgca cttaggtgat gaattatttg actgttttaa ttggacaatc ccttcataaa
    10321 tttctctaat atctgaaccc ttgaccctaa tggattcaaa tccaaatgct gaaaattttt
    10381 ctacgaaatc acctggatta caaatatcct ttgtaaaacc atctaattgt tttttgttat
    10441 catcaacaaa tacaattaag ttggataact gttgatgaga agcaaactgt atagcctccc
    10501 aacattgtcc ctcatttaac tcaccatctc caacaatagc ataagtataa aagggactct
    10561 ttcttattct ctgaccatat gcaagtccag ttgcaacact aattccttgt cctaaagagc
    10621 ccgttgtcat atctatgcct ggcgttagat ttctatcagg atgagacggt aatttggttc
    10681 catttgtatt taaagaatat aagaattctt tgtcaaagaa accattcaaa tagagtgtac
    10741 tgtatagagc tggtcctcca tgaccttttg ataatatgaa ataatctcta tctcgtgctg
    10801 caaatatttc tggagtcatt ggcattattt caccataaag cactgctaaa acttctacga
    10861 tagacagact tcctccgtaa tgtccgaatc caagatgatt caatgttcta agagtattta
    10921 atcggatgtt agtcgcaaat tttcttaacc catcttctct atttttactt aaaatcatcc
    10981 cttattcctc ctttgcagat ggctttttaa taaaggatac tccaaacata actgctagaa
    11041 taagaacaag accaatcaca atgcctgctt gtgagccaaa ttgatttaac attcctaaaa
    11101 taattcctga tagaccaaaa tctgcatctg agaaagttga tccttggaaa ccaagtcctc
    11161 ccaaaactgg cattaaaaag actggaagaa aactgattaa aataccttgt aaaaatgctc
    11221 caatagtggc tccacgaaca ccaccagatg cattcccaat gacacctgca gtcgctccac
    11281 agaagaaatg aggcacaaca cctggtaaga taacaaccgt tcctgaagca atcataattg
    11341 ccatacttac taaaccacca ataaaactag agataaatcc aattagaact gcattgggtg
    11401 cataagtata aacaatcgga caatccaaag caggttttga attaggtaca agacgctctg
    11461 aaataccttt aaaggctgga acaatttcgc ccaaaataag gcgaacacct gctaaaataa
    11521 caaatacccc tgctgcaaat tgacctgcta attgtaaagc ataaactaga ccacttgtac
    11581 cactactgat ttctttttct atatattctg accctgcaaa gatagctaca ataatgtaaa
    11641 taactgccat agataaagtg atactaacag tactatcacg taaaaaagct aaactctttg
    11701 gaaatttaat gtcctctgtt gattttgatt tgtcaccgat aaggctacca gtaaaaccac
    11761 tcaaccaata tcccaaagaa ctgaaatgac ctaaagccac cttgtcattt ccagttaatt
    11821 gaaccatata tttttgcaca aatgctgggg aaatactcat aataataccg agtgctaatc
    11881 ctcctagtaa gatgagaggc aagctagtaa agccagcaac tgataaaatg accgcaatca
    11941 tacatgccat atatagagtg tggtgccctg ttaaaaaaat atatttaaat cgagtaaaac
    12001 gagcgattaa gatattgaac accatgcctg caaacataat cattgcagta gctgagccat
    12061 atgttgttaa agctacagct acaattgctt cattattcgg cacaacgcca gataaatgaa
    12121 aagcatgctc aaacatggta ccaaatggat ttaaagaatt ttgtacaatt cctgcaccac
    12181 cagatacaac taagaaacca acaaaggtct taattccacc tttaataata tcaggtaatt
    12241 tcttcttctg aagaactaat cctaaaattg caattaaagc tactaaaata gctggtgtac
    12301 taacaatatc caatatgaac ttcatcatga cgctagcctc ctatataagt cctttttctt
    12361 cacaaagttt agtaattaat tctcgtagtt catccatatc aataatacta tttaagatac
    12421 gaacatctcc aagatgacta gctgaatcag ctagatcacg accaacaatc caaatatcag
    12481 ctgcatttgg atctgctcca cctaaatcat aatgttcaac ttctacatcc gaaacattca
    12541 aatcactcaa tacagattca atattcatct gtaccataaa acttgaacct aatcctgaac
    12601 cacaagctgt accaattttt aacattatct aatcctcctg tttaattatc attttaatgt
    12661 catcatagtt ttttgatgat attaaagttt gaacatgatt tttatctctt aaaattgttg
    12721 ttaaatgtga caaagccttt aaatgactct cattatcaat ggctgcaata caaatcaaca
    12781 atcttacctc ttgttctgga ttatccaata aataaatcgg ttcttccaaa actaacattg
    12841 acattcctat ttcattcaca ccttcatctg gccgagcgtg aggaattgct actcccttcc
    12901 ctaaattaat aaaaggtcca aactcttcta ctttttgaat cattgcctca gggtagttct
    12961 cagttatctt atcttgatcc aaaagcggtt tagctgctaa acgaatcgcc tccttccatc
    13021 ctaatttttg cgaactaacc tgataggttt ctttggtaat aagttgttct agcactggta
    13081 caatttcctt tctatcattt ttttggtaaa gataattctt taacgccaat cttaattcca
    13141 attcttgtgt aataattcca tatcttttga caatattcag gatttgttca atctcaaaat
    13201 ctccatactc taaattcgga aaatctttta acactagttc tactagttgt attgcttgct
    13261 cttcagtcat cataaccgaa actagataat ttggcttttc tgtctccacc tttatggtag
    13321 aaaaaaccat atcatagtca ctactagctt tcacctgtaa atcatcaatc tttgaggttc
    13381 ctataaactc aatttgagga aataatgcta atagattctc ttttaacatc aatgaagaac
    13441 taacaccatt aggacaaatg attgctgctt tatagcattt ttgaggcaaa gtatctgctt
    13501 tctttaaata acctccgaaa tggataacaa aatatgctgt ttcactatca ggtatgggat
    13561 tgtcaatagc gtccatcaag ggcatcaaag aatctttgac taattcaaat aaatcaggat
    13621 aatgttcttt aacatgcaat acatattcat ttgaactagg taggccgaac tttaatctat
    13681 agtaagccgg tataaggtgg cggcgaagat tttctctcaa tccttccctt tgtttaaaat
    13741 gtaacaaaga aatatcttcc attctactta taatagcctc tgttaattga ttaaagtaaa
    13801 ccggagcaac atctacttca ccttcaaagc aacttgataa taaaactgtg atatagcgat
    13861 aatcatcctc agaaaacacc gtatctataa ttcccaaatc aaccactgta tccaataaaa
    13921 tagtggttat atcttgaata acaggagata ctaatgtctc tgaaagacat actctttcaa
    13981 catccctttg atacctacac agaatgaata ctaaaccgaa aaggcaaact tttaattgat
    14041 taacaatagg tactagttgt agcttctcat aataatcttt aactacctga tttatcaaat
    14101 cataagttaa tgaatacccc caactggata aaacataatc caaaccccaa atccctatgg
    14161 aggattccag caactcacta accatttgaa aagctaagcg gtgcttattc cactctgaac
    14221 cgtgtaaagt ataacctttt gctctactgt accctagctc caaatcatta tctaacataa
    14281 tctttcttaa tgattgaata tcagataagg ttgtattctt acttactttc aaaaagtctt
    14341 ggtaatgatt attcgatata aaatctaatc ggcaaaaagt gtaaagatag attaaagcta
    14401 agcgagtcga ctttggtaaa accaattcat ccgacttaat aatatctgtc aaagactgct
    14461 tcgtacgatt tgataaacta tagcgacctt gttttttatc cagcactatc cctttattag
    14521 ctagataagg cactaaataa tctattcctt ctttgacttc ctttataggt aagctcacct
    14581 taacagataa ttcatataat gatagctcac aatgatccat caaagtcatc aaaataacta
    14641 gtgctctata atcaaacatt tctcttcctt tctaactaca attatagtgt aaaaattata
    14701 agaataaaag agtatttgag cacaatataa tctcaccttt taaaagaaat tattgggctg
    14761 aaagttttta gtgattattt tcaactttct attgactttt aaaaacaaag tgatatcatt
    14821 tagatatcaa ataatgggag gtcattctta tgactgaaaa actaatcaat tcaaaaccaa
    14881 atggtgtatt cgcattgatt ctcattgagt tgacaatcgt acttggtgtc tttatattta
    14941 taatgggtgt tggttcggaa aacatttttg gaattattat cggaccttta ctaatcgtaa
    15001 ttgcaggtct agctcatgct ggtttaaaag ttgtcaaacc tcaggaagct ctggttctga
    15061 cactctttgg taattataca ggtaccatca aagaacctgg tttttacttt gtcaatccct
    15121 tcagcgtagc agtcaaccct gcaaaccaca ctcgacttgg acaaagtggt gatgttagca
    15181 caaaatctcc ttttttagga gctaaatcat caaatgacaa tgatgtaaat cttgaaattg
    15241 gcaagaaaca catttccctc aaagtcatga ccttgagcaa ttctcgtcaa aaaatcaatg
    15301 attgcttagg aaaccctgta gaaatcggta tcgcggtaac ttggagagtt gtcgataccg
    15361 ctaaggcagt cttcaacgtt gataactaca aagaatatct ttcactgcaa tgtgatagcg
    15421 ccctccgtaa tattgtccgc atctatcctt acgatgtgtc tcctaatgtg gatactacgg
    15481 gtgacgggca agcagatgaa ggtagtctcc gtggctctag cgaaattgtt gctaaccgta
    15541 ttcgtgaaga aatccaaagt cgtgttgagg atgctggctt ggaaatcctt gaagcacgta
    15601 tcacttacct agcttatgct ccagaaattg ctgccgttat gcttcaacgc caacaagcat
    15661 ctgccattat tgatgcacgt aagatgattg tagatggtgc tgtaggaatg gttgaaatgg
    15721 cactagaacg gctcaatgaa ggggaattgg tagaacttga cgaagaacga aaagctgcca
    15781 tggtttcaaa tctccttgtc gttctttgtg gcaatcatga tgcacaacca attgtcaaca
    15841 caggaagtct ttactaaaga tggctgaagc taaaaaaaag cagatccccc ttcgactctc
    15901 aacaaagtta tacgctgcac tcgcatcatg ggcagaagac gactttcgtt cagtcaatgg
    15961 ccaaatcgaa tatctcctca cagagtgtgt caagcaacgg aagaaagacg gaaaatacgt
    16021 atcagaaacc attgacgaac catttgagat tgatatttaa caccttctcc tgtctgccgt
    16081 gtcggatagg agaatttttt catctttttt tgtcaagtaa ctttacttta caaaaaaaat
    16141 gtgttatcct agtatggttg atgaaaatca gtagattgaa tcgaatataa atacctaaag
    16201 gagaaatcaa aatggcagta cctgcacgtc gcacttcaaa agcgaagaaa aacaaacgtc
    16261 gtacacacta caaagtaaca gctccatctg taaactttga cgaaactact ggagattact
    16321 cacgttctca ccgtgtatca cttaaaggat actacaaagg acgtaaaatc gctaaagctg
    16381 catcagctga ataatagaag ggagatacca tgcgcgtaaa tattacactt gaacacaaag
    16441 aatctggtga acgcttgtac cttacttcta aaaacaaacg taacactcca gaccgccttc
    16501 aattgaagaa atactcacca aaacttcgca aacacgttgt gtttacagaa gtgaagtaag
    16561 cattcaatac gaatcaatac agaagaaaaa cgcttgatca gcgttttttc tttttgttaa
    16621 tttcactata ttgcaagaga aatcattcga atgactacat tttttgaaat aagaatacgg
    16681 attcggacga ttaaaagtaa gagaaactca aatgtaagtt ggggttcctt ttttgtatat
    16741 ttttcaaata acgcctctac actatttgta gcaaattcac caactacagt tgcatcttag
    16801 ttaaaataag ttagaatatg taagtgagta ccagatatac caagacatcg tcaccatcta
    16861 aggtatattc aaaatacaaa agttgaccaa ctagatttct gaatatcctt atatatccat
    16921 tcttaaaatt ggtttaaata gcgtagtctt ttaaactagt tttgagaatc caaaaaatct
    16981 tcctacatat gtaagaagat tttttagttc agaatgatta gatttagcta atggatacct
    17041 atcctaccca ctcaccgtta gcattgactc gatagccatc gggtgtagtc gtgtttacta
    17101 ataaatctcc tgaattatag gtatagtacc atttaccaga aactgtaaac caaccagtag
    17161 ccattgaacc tgattc
//
LOCUS       DAVKJU010000045        16532 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVKJU010000045 DAVKJU010000000
VERSION     DAVKJU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.045"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            119..2284
                     /locus_tag="VW191_001426"
     CDS             119..2284
                     /locus_tag="VW191_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEU9624179.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKINKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKNGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2284..3024
                     /locus_tag="VW191_001427"
     CDS             2284..3024
                     /locus_tag="VW191_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9624180.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3173..4660
                     /locus_tag="VW191_001428"
     CDS             3173..4660
                     /locus_tag="VW191_001428"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU9624181.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRLE"
     gene            complement(4706..5995)
                     /gene="ftsY"
                     /locus_tag="VW191_001429"
     CDS             complement(4706..5995)
                     /gene="ftsY"
                     /locus_tag="VW191_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU9624182.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEYIDPTFTEVEEVS
                     QEEAEVEIIEQAVFQEEEIQDTVEESLDSEPVVGVSQEEVEEFPHSEELPEEEKLEHE
                     GTVEESNSEVLEPEAPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDVLRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5999..6817)
                     /locus_tag="VW191_001430"
     CDS             complement(5999..6817)
                     /locus_tag="VW191_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9624183.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6817..7611)
                     /locus_tag="VW191_001431"
     CDS             complement(6817..7611)
                     /locus_tag="VW191_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9624184.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSHDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEDGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELVKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7608..11147)
                     /gene="smc"
                     /locus_tag="VW191_001432"
     CDS             complement(7608..11147)
                     /gene="smc"
                     /locus_tag="VW191_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEU9624185.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAVLEDKRNS
                     LSQEKSDKESSLVLLEENLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDVIAASAGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARKVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLRSDEVSLKNLQDELARLTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELNRLSEGEWQADKEKCQERLTTITSEKNNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVTDIERLGKELDNLDIEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQSQLTDQYQISYTEALEKAHELENLNLAEQEVKDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11138..11836)
                     /locus_tag="VW191_001433"
     CDS             complement(11138..11836)
                     /locus_tag="VW191_001433"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU9624186.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFNQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVIIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12019..12090)
                     /locus_tag="VW191_001434"
     tRNA            complement(12019..12090)
                     /locus_tag="VW191_001434"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12055..12057),aa:Arg,seq:ccg)
     gene            complement(12154..13140)
                     /locus_tag="VW191_001435"
     CDS             complement(12154..13140)
                     /locus_tag="VW191_001435"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEU9624187.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENIAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13315..14631)
                     /locus_tag="VW191_001436"
     CDS             complement(13315..14631)
                     /locus_tag="VW191_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEU9624188.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKILHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14618..16531)
                     /locus_tag="VW191_001437"
     CDS             complement(14618..16531)
                     /locus_tag="VW191_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEU9624189.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFSHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYISPEEVETDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQ
                     TGQQDTGDNDADY"
BASE COUNT         4803 a         3637 c         2917 g         5175 t
ORIGIN      
        1 aattcaagaa agaaaaggaa aaatcaatgt tatattttct gacacgaaaa atataaaaat
       61 ttgccttttt ttactttttc tgatataatg agagaatatt cggaaaagga gactaaaaat
      121 gaagaaaaaa tttctagcat ttttgctaat tttattccca attttctcat taggtattgc
      181 caaagcagaa acgattaaga ttgtttctga taccgcctat gcaccttttg agtttaaaga
      241 ttcagatcaa acttataaag gaattgatgt tgacattatt aacaaagtcg ctgagattaa
      301 aggctggaac attcagatgt cctatcctgg atttgacgca gcagtcaatg cggttcaagc
      361 tgggcaagcc gacgctatca tggcagggat gacaaagact aaagaacgtg aaaaagtctt
      421 caccatgtct gatacttact atgatacaaa agttgtcatt gctactacaa agtcacacaa
      481 aattaacaag tacgaccaat taactggcaa aaccgttggt gttaaaaacg gaactgccgc
      541 tcaacgtttc cttgaaacaa tcaaagataa atacggtttt actattaaaa catttgacac
      601 tggggattta atgaacaaca gcttgagtgc tggtgccatc gatgccatga tggatgacaa
      661 acctgttatc gaatatgcca ttaaccaagg tcaagacctc catattgaaa tggatggtga
      721 agctgtagga agttttgctt tcggtgtgaa aaaaggaagt aaatacgaac acctggttac
      781 tgaatttaac caagccttgg ctgaaatgaa aaaaaatggt agtcttgata aaattatcaa
      841 gaaatggact gcttcatcat cttcagcagt gccaactaca accactctcg caggattaaa
      901 agctattcct gttaaggcta aatatatcat tgccagcgat tcttcttttg caccttttgt
      961 tttccaaaat tcaagcaacc aatacactgg tattgatatg gaattgatta aggcaatcgc
     1021 taaagaccaa ggttttgaaa ttgaaatcac caaccctggt tttgatgctg ctatcagtgc
     1081 tgtccaagct ggtcaagccg atggtatcat tgctggtatg tctgtcacag atgctcgtaa
     1141 ggcaactttt gacttctcag aatcatacta cactgctaat accattcttg gtgtcaaaga
     1201 atcaagcaat attgcttctt atgaagatct aaaaggaaag acagtcggtg ttaaaaacgg
     1261 aactgcttct caaaccttcc taacagaaaa tcaaagcaaa tacggttaca aaatcaaaac
     1321 ctttgctgat ggttcttcaa tgtatgacag tttaaacact ggtgccattg atgccgttat
     1381 ggatgatgaa cctgttctca aatattctat cagccaaggt caaaaattga aaactccaat
     1441 ctctggaact ccaatcggtg aaacagcctt tgccgttaaa aaaggagcaa atccagaact
     1501 gattgaaatg ttcaacaacg gacttgcaaa ccttaaagca aacggtgaat tccaaaagat
     1561 tcttgacaaa tacctagcta gcgaatcttc aactgcttca acaagtactg ttgacgaaac
     1621 aacgctctgg ggcttgcttc aaaacaacta caaacaactc cttagcggtc ttggtatcac
     1681 tcttgctcta gctcttatct catttgctat tgccattgtc atcggaatta tcttcggtat
     1741 gtttagcgtt agcccataca aatctcttcg cgtcatctct gagattttcg ttgacgttat
     1801 tcgtggtatt ccattgatga ttcttgcagc cttcatcttc tggggaattc caaacttcat
     1861 cgagtctatc acaggccaac aaagcccaat taacgacttt gtagctggaa caattgccct
     1921 ctcactcaat gcggctgctt atatcgctga aatcgttcgt ggtggtattc aggccgttcc
     1981 agttggccaa atggaagcca gccgaagctt gggtatctct tatggaaaaa ccatgcgtaa
     2041 gattatcttg ccacaagcaa ctaaattgat gttgccaaac tttgtcaacc aattcgttat
     2101 cgctcttaaa gatacaacta tcgtatctgc tatcggtttg gttgaactct tccaaactgg
     2161 taagattatc attgctcgta actaccaaag tttcaagatg tatgcaatcc ttgctatctt
     2221 ctatcttgta attatcacac ttttgactag actagcgaaa cgcttagaaa agaggattcg
     2281 ttaatggcaa aattaaaaat tgatgtaaat gatttacaca agcactatgg aaaaaatgaa
     2341 gtcctaaaag gaattacgac taagttctat gaaggagatg ttgtttgtat catcggtcct
     2401 tcaggttctg gtaagtcaac tttcctccgt agcctcaatc ttttagaaga agtcactagc
     2461 ggtcacatca ctgtgaacgg ctatgattta actgaaaaaa caaccaatgt tgaccacgtc
     2521 cgtgaaaata tcggcatggt attccaacac ttcaacctct tccctcatat gtctgtattg
     2581 gacaacatca cctttgctcc tattgagcac aagttgatga ctaaggaaga agctgaggaa
     2641 ttgggaatgg agttgcttga aaaggttgga ctagcagata aagctaatgc caatccagat
     2701 agcctatcag gtggtcaaaa acaacgtgtg gccatcgctc gtggcctagc aatgaatcca
     2761 gacatcatgc tcttcgatga accaacttct gcccttgacc ctgagatggt tggagacgta
     2821 cttaacgtta tgaaggaatt ggctgagcaa ggcatgacca tgattatcgt aacccatgag
     2881 atgggatttg ctcgtcaggt tgccaaccgc gttatcttta ctgcagatgg cgagttcctt
     2941 gaagacggaa cacctgacca aatctttgat aacccacaac accctcgtct gaaagagttc
     3001 ttagataagg tcttaaacgt ctaaactcaa actgtaagga tttccttgca gtttttctac
     3061 ctcgtattgg aatttttgat ttttcggaaa attatgttag aattaagttt atgaaatgag
     3121 gtttcctcat acctagcaag actaggaata aaaatagaaa ttaggtagct agatgtcatc
     3181 taaggttatt gttacaattt tcggtgcgag tggagacctg gctaaacgca agctctaccc
     3241 ttcccttttt agactatata aatccggcaa tctttccaag cactttgccg ttattggaac
     3301 tgcccgtaga ccttggagta aggaatattt tgaatctgta gttgtcgagt ccatccttga
     3361 tttggcagat agtaccgagc aagcccaaga atttgctagc cacttctact atcaaagcca
     3421 tgatgtcaat gattcggaac attatattgc tttgcgtcaa ttacaagctg agcttaatga
     3481 aaaataccaa gctgaacaca ataagctctt cttcttgtct atggcacctc agttctttgg
     3541 aaccattgcc aaacacctca aatctgaaaa cattgtcgat ggcaaaggtt ttgagcgctt
     3601 gatcgttgaa aaaccatttg gtacagatta cgcaactgca agcaagttga atgacgaact
     3661 cctagcaaca tttgacgaag aacaaatttt ccgtatcgac cattatcttg gtaaggaaat
     3721 gatccaaagc atctttgcag ttcgctttgc aaacttgatt tttgaaaacg tttggaacaa
     3781 ggattttatc gacaatgttc aaattacctt tgcggagcgc ttgggtgtag aagaacgtgg
     3841 tggctactat gaccaatccg gtgccctccg tgacatggtc caaaaccaca ctctacaact
     3901 tctttcgctc ctcgccatgg acaaaccagc aagcttcaca aaagacgaga ttcgtgctga
     3961 aaagattaag gtctttaaaa acctctatca tccaactgat gaagaactca aagaacactt
     4021 tatccgtggg caataccgct ctggtaagat tgatggcatg aaatacatct cttatcgtag
     4081 cgagccaaat gtgaatccag aatcaacaac tgaaaccttt acatctggtg ccttctttgt
     4141 agacagcgat cgattccgta gtgttccttt ctttttccgt acaggtaaac gactgactga
     4201 aaaaggaact catgtcaaca tcgtctttaa acaaatggat tctatctttg gagaaccact
     4261 tgctccaaat attttgacca tctatattca accaacagaa ggcttctctc ttagcctaaa
     4321 tgggaagcaa gtaggagaag aatttaactt ggctcctaac tcacttgatt atcgtacaga
     4381 cgcgactgca actggtgctt ctccagaacc atacgaaaaa ttgatttatg atgtcctaaa
     4441 taacaactca actaacttta gccactggga tgaagttggt gcgtcatgga agttgattga
     4501 tcgtattgaa aagctctggg ctgaaaatgg tgccccactt catgactata aagctggaag
     4561 catgggacct caagccagct ttgacctact tgaaaaattc ggtgccaaat ggacttggca
     4621 accagatatc gcctatcgtc aagatggtcg cttagaataa aaaaatttcc tgcaagttta
     4681 tgccttgcag gatttttgct tctgattaga ttaaaccttc caagagacct ttcataaagt
     4741 tttctgagtt aaactctcca atatcatcga ttttttcacc aaaaccaatc aattttacag
     4801 gaatattgag ttcttcacga atggctagaa ccacacctcc tcgagcagtt ccatcaatct
     4861 tagtcaaaac aattccggtt aaaggtgtga ttttcgaaaa ttctttggcc tgtactaggg
     4921 cattttgacc tgttgatgca tcaagtgcca agaaggtttc atgtggtgct tctggcacaa
     4981 cacgtttgat aatacgacca atcttttcca actcagccat aaggttatcc ttattttgca
     5041 gacgaccagc agtatcaatc atgagaatat cgataccttc agccacggca cgttccatac
     5101 catcaaagac cacgctggct ggatcagctt tttcaggtcc agttactact ggaacatcta
     5161 ctcgtcggcc ccattcagct agctgagcta ctgcacccgc acggaaggta tctgctgcaa
     5221 ccagcatgac cttcttacca gcttgtttgt agcggtgggc tagttttccg atagaagttg
     5281 ttttcccaac accattcaca ccaacaaaga gcataactgt caagttatct tggaagtgga
     5341 tgctttcatc gtagctacca tccttttcat aaagctcaac caatttctca atgatgacac
     5401 gacgaagtac atcaggtttc ttggcatttt caagcttggc ttcgtaacgt agttcctccg
     5461 ttaagttaga agcgacttgg acaccaacat cactcataat cagcagttct tccagttcct
     5521 cgaaaaattc ttcgtcaaca gagcggaagt tagcaaagaa ggcattcaag cgggcaccga
     5581 aacctgtgcg agttttctta agactgcggt catatttttc ctgaacagtt tcttctgttt
     5641 gaggagcttc tggttcaagc acttcagaat tactttcttc tacagttcct tcgtgctcaa
     5701 gcttctcttc ctctggtaat tcttctgagt gtggaaattc ttcgacttct tcttgagaaa
     5761 cccctacaac tggctctgaa tccagacttt cttcaactgt gtcttggatt tcctcttctt
     5821 ggaacacagc ttgttcaata atttcaacct ctgcttcttc ttgagaaact tcctcaactt
     5881 ctgtgaaggt aggatcaata tattcagaca aatcaagatt ttccagagct tcttttacaa
     5941 cttcttcgat tttaggttct tctttttttc cgaatagacg gtcaaataat cccatatctt
     6001 agttctcctt tagcacatat tcttcgatag cccaggcgac agcttcttca tcattggtca
     6061 taggagttac cacatttgcg actgccttga cttcaggaac agcgttttgc atggcaacac
     6121 cgagccctgc ccattcaatc atagagaggt cattggcctc gtcaccacag gccatcactt
     6181 gactttgatc aattccaaga tggctaatta gttttgccaa acctgttgct ttatgaacat
     6241 tctttggtga ccattctagc aacatttcac gtgatttaaa gatttcatat tggtcaaaca
     6301 attctggaga aatcttctga atggctgcat ccaagggttc ttgagcaaag gcggtcacgc
     6361 atttgttgta ggtcatttga ttagacaggt cttcaaaatc aactggaaca aaagtcaaag
     6421 ctggattgaa tttggcataa agactttctt ggtccgattg aatttgataa acagttcctt
     6481 ctgagatagc gtcaagaggc agtgataatt tctctgtttc ttcatacaag cgtgccacat
     6541 catcatatga aaagactgtc ttatcaagga tttcacctgt atttttctga accaaaccac
     6601 cattaaaagt aatggtatac tcatcttcct gaccgtcagt ccctagctca tggagaaaga
     6661 aatccatagc ttttaaggga cgaccagttg tcaatacgac cttgatacca cgatcacgcg
     6721 cagctttcaa ggtttccttg gtacgatccg tcagcctttt atcagtagtc agcaaggtcc
     6781 cgtccaagtc caatgcgatt aattttatat ctgccattat aagccctcca tataagctat
     6841 aaccgactgg tccttatggt gaccaatcac agtctttgct aattctaaaa tttcaggtcg
     6901 tgcattttca ggagctacag gatgtcccac aacctgcatc atatgtaagt cattaagatt
     6961 gtctccaaaa gccatgacct gatccattgt gataccaagt tttttaacta attcaacaat
     7021 ggccactccc ttatcgacat agtccagaac aatatcaata gattcaaagc cagttgtcat
     7081 agccttaaca ccaggaacgt tttcgtttac ccaagcctcc ccatcttcca gcgtttcttc
     7141 tgtgaagttg gttgtaaatt tgaaaatgtc atctgtgata tcttccaaac tcgctacttt
     7201 ttggatattt tcattataat gctgactcac tttcaaatag gtctcatcaa ccgtatctag
     7261 aacatatgaa cccttcttac ccgtcaagag cagtttattg atatctacat aaggtgacgt
     7321 tttcagcttt tcaaaagttg ccagataaaa gtcatgagac atagtcgctt catacaagtc
     7381 ctgaccttga tactctacca aactgccatt ttccgcgatg aaaataatgt catcacgaac
     7441 atcagcaaat aatttttcca gagacagaaa tccccgaccc gaagccaccg caaagtaaat
     7501 ccctttttcc ttgtaggaag ccaagagaga cttgagacga tccatatcaa agcgtccatt
     7561 cccatctagg aaggttccgt ccatatccgt tgctactagt ttaattgtca tccttcaata
     7621 ctttctaaat cttttaactt aactgaaaca atttttgaga cacctgattc ttgcatggtc
     7681 actccataga tagaatcagc cgctgccatg gttcccttac ggtgggttac gacgatgaac
     7741 tggctgtcct tgtcaaagcg gttgaggtaa tccccaaaac gtttaacatt agcttcatcc
     7801 agcgcagctt ccacctcatc caagataaca aatggaatag tcttgacacg aataatggag
     7861 aagagcaagg caagagccga tagagctttt tcaccaccac tcatgaggtt aagcgactgg
     7921 attttcttac ctggaggttg aacagaaatc tccacaccag ctgttaaaag gtcgccctca
     7981 gtcaatatca agtctgcctg acctccgcca aacatctgct tgaaggtcac tttaaaggac
     8041 tcacgaatag cttcaaaggt tgatttaaag cgttccttga cctcatcatt catctctgta
     8101 atggtttcaa ggagcagatt tttcgctgac aaaatatcat ctcgctgact atttagaaag
     8161 tccagacggt tgtgaacttc ttcgtactgg tcaatagctt ctatattgac aggacccagt
     8221 gagcgaatag ccttctctaa atccttaact tcttgctctg ccagattgag gttttccaat
     8281 tcatgcgctt tttctagagc ttcagtatag ctaatctggt actggtctgt taattgactt
     8341 tgtagatggc gcaagcgttc gctgaccttt tctttcttag cttcagcacg tgtttgcttg
     8401 cgaatccact cctcattctg ctggcgagcc tgatccaaat gactagcaat atcatccagc
     8461 tgaccttcta tatcatccaa ctcaaactgt ttgcgaatca aaccttgttg gaggttcgtt
     8521 ttctgagttt tggattcttc cgcctgttga ctgagcaatt ctgtatcaac cttctcaaga
     8581 ttgtcaacct tttcttgaag aaggcgctga atttcttctt gctcaatatc aagattatct
     8641 agttccttac ctaagcgttc aatatcagtc acttcataac gtttttgccc ttgcagttct
     8701 gtcttaagta gacgagcttg agctagctct tcctgcaagt tttgataacg ttcttggatg
     8761 gcgtttttat tagacttaat ctcttcaatc tcagcttcca gattattctt ttcactagtg
     8821 attgtagtaa ggcgctcttg gcatttttcc ttatccgctt gccattctcc ctcagaaaga
     8881 cgatttaatt cctcttcttg gagtttccaa agagtttcta gctcttcaac ttgctgactg
     8941 gtctgctgat aggcgaggga caagccttgc tcctgaatac gagcctgctc tccctgagat
     9001 ttgatggctt ctaatctttc tgtcaatcta gctagctcat cttgcaagtt cttcaaactc
     9061 acttcatctg aacgcaaact tgcttcttct tcagcaattt ctttttgtaa ttgctccagt
     9121 tctggcttga tgaaaatact gttattctga cgattggctc cacctgcgta agaaccacct
     9181 gtgcgcaact ctgtaccgtc caaagtcacc atacgaacct gataacgaac tttgcgcgct
     9241 gctgcacgcg catgctcaac ggtatcaaaa atcgctgtcg tagctagcaa gttcttgaaa
     9301 atagcttcca gtctcgtatc gaatgtcacc aactcatctg ccatgcctag aaatcccgca
     9361 cttgcagcga taacatcttg gttctgacta gaaatcgtac gcgccttgat agtcgtcaac
     9421 ggaaggaagg ttgcacgacc agctctattt cgtttgagga aatcaatcgc cttggttgcc
     9481 gactcttcat cttctacgat gatatgctga ctgctggctc caagtgcaat ctctaaggca
     9541 gtttgataat gcacatcaaa ggtcaaatgc tcactaacag caccaataat cccaccaagg
     9601 cggtcttttt cttggagaac actctttaca cctgcataaa agttactgtg atttctaagg
     9661 atgttttcca aactttgagc tctagcctgc ttgtttttaa gactatccag acggtcaaag
     9721 agttgacttt gttgagcttg ataggaagtt ttttgctcct cttgctcctt ggcaatagct
     9781 tgatagtcag ccaataattt ctgaacctgc accttggcag tttcaagctc gtctttttgc
     9841 tgactagcct tctctttagc tgtagctaat tgctctttca gcttttctag ttgatctgct
     9901 tgtttttgag aaagctgacg actattttcc aactcattct cgatgcgggt cagttggttt
     9961 gagacatccg cttcttcttg taaaagcgcc acaaagcgtt cacgcaaaag ctcaatcatc
    10021 tgatcaggat catctgaaaa agctagcaat tcagcttcta aacgattgag tttttgatta
    10081 ttttggacta gattttcctc taaaagtact aaagagcttt ctttatcaga tttttcttgg
    10141 ctgagtgaat ttctcttatc ctccaaaaca gccaaacgag cttgtgcttc ctgttgattc
    10201 agggctactt gctcagattc taatttcgat agggctaatt ttctctctaa atcactaatc
    10261 agactggtca aatccatcaa actgccttgg tctttggcca tttcagcctg taaatcttgg
    10321 cgttgctttt taagagtttg attttcttct tctaattttt cacgcttttg gtaataactc
    10381 atcaagagtt cttgaacctg agccaactct tcttctgtcg actctagttc agccttattt
    10441 tccttgattt gagcaaccag aacatctaaa taaatagcct tacgttgtcc ttccaagtct
    10501 aaaaacttac gggcattctc agcttgcttt tcaagaggct tgatttgatt atccaactcg
    10561 tagataatgt cctctaagcg gtccagatta tcctgagttt gctgcagttt actctcggtt
    10621 tcttttctgc gagtcttgta ttttaaaact ccagcagctt cttcaaaaat agctcgtcgt
    10681 tcctcaggct tggaattaaa aatctcctca accttccctt gggaaataat agagaaggaa
    10741 tctcgtccca atccagtatc caagaagagg tcatgaatat cacgcagacg gactttcttg
    10801 ccgtcaatct tgtattcgct atctccacta cgatagatat ggcgttccac cctgatttct
    10861 tgacctgcat ccttgataaa tccgtcatga ttatccagag tcacaactac agaagcataa
    10921 ttgagcggtt tgcgactttc ggttccagca aagatgacat ccggcatctt gcccccacgg
    10981 agactcttga cactagactc ccccaaagcc caacgcagac tttctgtaat attggacttt
    11041 ccagatccat tgggtccaac aactgccgtc acaccttggt caaaaacgac cttggtctta
    11101 tcagcaaaag acttgaaccc ctgaatttcg atttccttta aatacatgaa tccagcccct
    11161 tctcaacggc atttttggca gcttcctgct ctgctaattt cttagaacga ccttggcctt
    11221 gaccgatgct cttaccttca acaagaactt ctacatcaaa gaccttatca tgagcaggcc
    11281 ctgtttcaga aatcacctga taacgaatag ccacatcacc attgacctga agcaactctt
    11341 ggagatgggt tttatagtct gtaatcatct caaactcgcc tgcttcaacc ttaggaatga
    11401 tgacttgata gataaattcc ttgaccttgg ccacatcctt atccaaaaga agggcaccaa
    11461 gaaaggcttc aaaggcatca ccaagaatgg tgtcacgatt gcgaccacct gatttttctt
    11521 cccctttacc caacttgata aactggttaa actggcaatc acgcgcaaaa ccagctaaac
    11581 tctcctcacg gacaatcata gcacggagtt ttgataggtc accttcaggc tttttaggat
    11641 attttttata tagatattct gaaatcaata actgtagaac agcgtctcct aaaaattcca
    11701 agcgttcatt gtgtgaaatt tttaagaggc ggtgctcatt ggcataactc gtatgagtaa
    11761 aggcagtttc cagtaaattt ttgtctgcaa attcgattgc aaaatgattc tttagtacag
    11821 tttgtaattc tttcatacca acctctttct aactgataat agtccttttt attatatcaa
    11881 aaaaagcccc ctgagtcact ctaaaacggg actggaaagc atttgggaat tctttagaca
    11941 gagattctca gttttagcgg caaatttggg tcaggataaa gaaaaaagcc ctattaaagg
    12001 ctttttagga tgtttacatc caccctgagg gaatcgaacc cccatctcaa gaaccggaat
    12061 cttacgtgat atccattaca ctaagggtgg aaacttgttt tattataaca gaaatttgct
    12121 ctaataacaa gttttttggt caaagacccc gtcttagtgg gaagcatccc cattccagat
    12181 ggagtttttc acgatcacat aatcaacgtg tttaaggtca gcaacctgac gtccacctgc
    12241 ataagaaata gcactttgaa ggtcttgttc catctcagtt aaagtgtctt gcagatgacc
    12301 tttagcagga agcaagatac gtttgccttc cacatttttg taagcacctt tttgatattg
    12361 tgaggctgaa ccataatatt ctttgaactg ttcaccatcg acttcaatcg ttttccctgg
    12421 actttcaatg tgtcctgcaa agagggaacc aatcatgatc atgctagcac cgaagcggat
    12481 agacttagca atatcaccgt gagtacgaat tcctccatca gcgataatcg gtttacgcgc
    12541 agccttggca caccagcgta gagcagccaa ctgccaacca cctgtaccaa aaccagtctt
    12601 aaccttggtg atacaaacct taccaggacc gattccgacc ttagtagcat ccgcaccagc
    12661 attttccaat tcacgcacag cttctggtgt tcccacattt ccagcaatga caaaggtatc
    12721 tggcaattct ttcttgatgt gttgaatcat agaaatcacg ctatccgcat gaccatgagc
    12781 aatatcaata gtgatatact caggagcatc agccttgagc tgtctaacga aatcatactc
    12841 ataatcctta acaccgacag agatagaagc aatgagccct tgatcgtgca ttcgtttaat
    12901 aaaaggaatg cgtcctgcct catcaaaacg gtgcataatg tagaagtaac cacctttagc
    12961 cagttgctct gctatatttt catccaaaat cgtctgcata ttcgctggca caacaggtag
    13021 tttaaaggtg tgatttccta aagtgacact tgtatccgct tctgcacggc ttttaatgac
    13081 acatttattt ggaatcaatt gaatatcttc gtaatcaaaa attggaaatt catttaacat
    13141 atcgatgtct cgtttctttt gtaatgacct acctatgctc ttgcatcact acgccttttc
    13201 cgacgtttcc ttaatttatt ataaaccaaa gtacagtttt tgtcaaatta ttttatgaat
    13261 tgaaatatat tgttcgggtt ttacttgtgt taaaaataga agaaagagag gaaattattt
    13321 tctccccact tcttcatcaa taatcgcttt aaaattttct tctgaatttt ccatcatttt
    13381 acaaaatgca ctataagatg aagacttctg aggaatttgg atagaccatt tcttcaacaa
    13441 agctccataa cctgctaggt ttcccatctc attatcaata accttgtcct tgctcggaaa
    13501 aacaagtcca gccttctcag cttttaaaat ataagaatag gaaatcagct ggaataagtc
    13561 ctcacggttg attccttttt cagtcaattc cagtttttta tattttgcat ctagaacaat
    13621 ctttcgttct ctgtcataaa aatctggata tacttttcgt ttcccaacag aaaatactga
    13681 aattccatcc gtcttatcct tatttctggg atgtacaaaa ccttttggca acaaggtgta
    13741 aacatactct tcccaaagcc aggcaacatc aaagagaata ccatagattt tttgatcttg
    13801 ataccctaaa ccgtgctttt cttggtttag gatcatcaga caaagttctt gtaagtttct
    13861 gtactcgtga aagtatgcat gacgtatagg tttagattga tttccccgaa taatcttagc
    13921 acgatcagct agtttataag agggcgttac acgcacgatt tcagatacgt tttcacgact
    13981 agttgagaga ttatctagta ccccttgacc aatgcttttc tgattcttaa tgtattcaat
    14041 agtgtgacgg accaactgca tgaggggatt atcataggtg aactctctcg ttgcgtaggc
    14101 aatatttccc gtgaaaggaa gatttttctt gagatggttt cttacatcaa tcactccctt
    14161 aacatgactg tcgttatgag aaaatcgatg atattcctta taaagacctt ttcgaatagc
    14221 agcttgtaga tacttgggaa agagatacac caaaagttga taaagcctct cttcacgaga
    14281 caaagcaaca tctaaactag tgagattgat atgaagaatc ttgtttaaga gataatgcaa
    14341 aaaatggtca ttactctcat cagaaaatcg tgaggaaatc gttaatcttt cctgaccata
    14401 tccaagaaaa ccaatcacgt tccctgtctt gattttctga ttgactgttt caaaaatctt
    14461 ttggtcctta tccaaatcag gagtatgagt caaatcattt ggaaaaatga aaatacgttc
    14521 atcttgggat agactttcca aggttctgtc taaaagaaga ttgcttagtt taggatattc
    14581 ttcaacaaat ttttctttaa taatcttgta ttgattatca gtaatccgca tcattatcac
    14641 cagtatcttg ttgaccagtt tgatcattat ttgtcagttc aaatgctttt ttcaaagttt
    14701 ccaaagtttc aacctcatca taagaacctc gcaagtagtc ttctaggaga ggcttaatat
    14761 aatcagacca gagtaattca tagtcaaaat ctacatcctt caacttaaga aaataacttg
    14821 gtccaatatg ataatgactg tttaattcct gaacattttc gatagcagcg ttcaagtttc
    14881 ttagacgaat ttttgcttct tctgcatgga tattcagctc tttatccaac atgccaactt
    14941 gaccctcggc agtaacttca acaaaacgaa aacgacgacg catagcaaaa tcaaaggtat
    15001 ccactgaacg atcaatatca ttcatagttc cgatgatgta aacattttcg gggatataga
    15061 acttttcatc agtttcgtgt agatttgcat attgggtaga aacacttcct ttttcaccac
    15121 gatagccagg gtcgatagag aaaaagagtt cgccaaaaat cttagaaatc tccccacgat
    15181 tgatctcatc gatgatgaaa acaaatttct tgtctgtttc aacctcttct ggagaaatat
    15241 agtctttcaa accaaatctc tttctcaatg tttctaggac agtttttcca ccacttttgt
    15301 agtattcttg cttattataa tttttatctt tatacaactc gtaaacatat ttgctaggta
    15361 gtgaccatcc tggaacacca ctatcataat ttactgacaa attttgtcta ctattaacag
    15421 ataagtaaga tgtttttgtt atatattctt tttcttcagc aacatttata tattctaagt
    15481 aagaatccca agcctcatca aaattatctt gtcctccaat caattgggtt tcttttgctt
    15541 tctgacaaaa atctttaaaa ataccgtcct gtagcctaaa ctcaatagct ccatccccat
    15601 ttgatactgg tcttaaacct tctacaaaat ccgtataatc ataggatggg tgaaattgta
    15661 caaatccgat ttggtcttcg ttaccatccg ttaattcttt ggcaatttct ttagcaagat
    15721 aagtctttcc tgtgccagga gcaccacgaa ggataaggtt tggagacttt aatagagatt
    15781 gagttaattg atgactaaaa ttgtttgatg aatccattgt actattgtct tccttatttt
    15841 ccataaaaag cttagaatac tcaaaaagca tagttcctaa tttcgatggc tccaactcag
    15901 gataagtttt ggacaccagt tcaattattt tatgattttg cagataaggt ccaccttgat
    15961 ttgtctgaga aacaaagtgg ttccaaaatt ctttctgact attaatattt acaccaaaga
    16021 aaggatctcc ctcagtatag atacaaagta attttgtcac catagcagaa cgaccaataa
    16081 attcatttgt tgatcttttc atatcaaaaa taggattttc aaagttaaaa cgaatacctt
    16141 ctttgataat ttcatacaaa tctgatttta attttgaaaa tcgctgatcc aactctgaaa
    16201 tctcattatt agcttgatct ttatatttgt ttgttttttt attccaataa ataccaaatt
    16261 ttgaagctga gccaccagaa attccaagaa atagattttt gtattttccc ttctcaagag
    16321 cgtagcataa agacttattt tgctgtccct ttcctattac atattcatcg attgacattg
    16381 ttaaaatcct atccaaaggc cattcctgga gaaactcttc ttttaattta tcattaaact
    16441 tttcaccaac agttgcctta tttaataact cttctttatt tagactatac catttataaa
    16501 aatcttctaa actaactttt cttttattca tc
//
LOCUS       DAVKJU010000046        16475 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVKJU010000046 DAVKJU010000000
VERSION     DAVKJU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16475
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.046"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..739
                     /locus_tag="VW191_001438"
     CDS             <1..739
                     /locus_tag="VW191_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9624190.1"
                     /translation="NVINELNKTENGRLIIRRIATEFYKMKNIPDEVQDRDRGLDALR
                     KLKRLIGDTQQNKVNETLNNSYHRSRQEMKIQLRQQLLQKIEELKTEYYSLFSSDNPQ
                     ERGYRLEKIVANLFRINDIDYHDSYRNRTNTQQLDGYFRFEGFDYLVEMKWEKNPVNS
                     PKIASLKQKVDTKLTSTRGLFLSINGFRDEVIQDFSNKDAKILFMDGQELAYILENRI
                     SLYEALKVKIIGASKTGNPNVSIINQE"
     gene            1142..1380
                     /locus_tag="VW191_001439"
                     /pseudo
     CDS             1142..1380
                     /locus_tag="VW191_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1524..2753
                     /locus_tag="VW191_001440"
     CDS             1524..2753
                     /locus_tag="VW191_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEU9624191.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2773..5469
                     /gene="ppc"
                     /locus_tag="VW191_001441"
     CDS             2773..5469
                     /gene="ppc"
                     /locus_tag="VW191_001441"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEU9624192.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5552..5769
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5882..6886
                     /locus_tag="VW191_001442"
     CDS             5882..6886
                     /locus_tag="VW191_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9624193.1"
                     /translation="MKNKRLIGIIAALAVLVAGNLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSIGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6955..7821
                     /locus_tag="VW191_001443"
     CDS             6955..7821
                     /locus_tag="VW191_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9624194.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7818..8576
                     /locus_tag="VW191_001444"
     CDS             7818..8576
                     /locus_tag="VW191_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624195.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8666..10426
                     /gene="dnaG"
                     /locus_tag="VW191_001445"
     CDS             8666..10426
                     /gene="dnaG"
                     /locus_tag="VW191_001445"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU9624196.1"
                     /translation="MVNKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10429..11538
                     /gene="rpoD"
                     /locus_tag="VW191_001446"
     CDS             10429..11538
                     /gene="rpoD"
                     /locus_tag="VW191_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU9624197.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11552..11881
                     /locus_tag="VW191_001447"
     CDS             11552..11881
                     /locus_tag="VW191_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU9624198.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11996..13039
                     /gene="cpoA"
                     /locus_tag="VW191_001448"
     CDS             11996..13039
                     /gene="cpoA"
                     /locus_tag="VW191_001448"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEU9624199.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKEIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            13055..14380
                     /locus_tag="VW191_001449"
     CDS             13055..14380
                     /locus_tag="VW191_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9624200.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFSDVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDVNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14411..14587
                     /locus_tag="VW191_001450"
     CDS             14411..14587
                     /locus_tag="VW191_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU9624201.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14646..14780
                     /locus_tag="VW191_001451"
     CDS             14646..14780
                     /locus_tag="VW191_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEU9624202.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14844..16154
                     /gene="obgE"
                     /locus_tag="VW191_001452"
     CDS             14844..16154
                     /gene="obgE"
                     /locus_tag="VW191_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEU9624203.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            16164..16322
                     /locus_tag="VW191_001453"
     CDS             16164..16322
                     /locus_tag="VW191_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624204.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
BASE COUNT         5134 a         2848 c         3651 g         4842 t
ORIGIN      
        1 aaatgttata aatgaactta ataaaacaga aaatggaaga ttgataatta gacgaatagc
       61 aactgaattt tataagatga aaaatattcc tgatgaagtt caagatagag atagaggatt
      121 agatgcgtta agaaaattaa agcgactgat aggtgatact caacagaata aggttaacga
      181 aacacttaat aattcttatc atcgttccag acaggaaatg aaaatacaac tcaggcaaca
      241 actccttcaa aaaattgaag aactaaagac ggagtattat tcgctatttt caagtgataa
      301 tcctcaagaa agaggatatc gtttagaaaa aattgttgct aatttattca gaattaatga
      361 tattgattac catgattcgt atagaaatag aacaaatact caacagttag atggctattt
      421 taggtttgaa ggttttgatt atttagttga aatgaaatgg gaaaaaaatc cagtaaattc
      481 tccgaaaata gcttctttaa aacaaaaagt tgatacaaaa ctaacttcga ctcggggact
      541 gtttctttca ataaatggtt ttagagatga agtaatacaa gatttttcta ataaagatgc
      601 taaaatttta tttatggatg gacaagaatt agcttatatt ctagaaaata gaattagttt
      661 gtacgaagct ttaaaagtta agataattgg tgcttctaaa actggcaatc caaatgtttc
      721 tataattaat caagaataac ttttccgaga gatattaaaa ccatcaataa aatagagcga
      781 tactttatat gccatgatac aaatgatata caatgatact tctgaccgtt caggctgcca
      841 tcgtaaaaga gcagcaagtg aaattcttat gatgacttca tcagtcatgc cacgttaaat
      901 gtgtgagttt gttagagaaa cgcaattaat cctcaaaagg ttccccgaac cttttgagtt
      961 ccacagacgc atcacgtgga gtgtgtaagc ttgttgctaa aagcgtaaaa accttggaac
     1021 gaaaggaata atagacttcc tgcgaaacaa aaatacaata caataaaact atggatgatg
     1081 aagcaagtaa acaattaagc gatagccgtt tcaagagcct tgtaggtgtt cagcgcacga
     1141 gatgttagct gtgttaaaaa cagcttatca acgcaacgta aacgcgcaaa aggtggacga
     1201 aaaaccaaat taagcctaga cgatctcctc atggtaacta ttcaatacat gcgagaatag
     1261 cacacttatg aacaaattgc ggctgatttt ggcattcacg aaagcaacat aattagtcgg
     1321 agtcaatgga ttgaagcaac tcttattcaa aatggtttta cgatttcaaa ttctgcctta
     1381 attctgtaaa aacagtaaaa ttcgaaggat tgtaaggtaa gagttttttt ctttctgaaa
     1441 aaatggtata atagcaatca aaactagaaa ataaaacgga atttggaaca gatttgtctg
     1501 tatcctagta gagtggtgat actatgaaga ttagtaagag gcacttatta aattattcca
     1561 tcttgattcc ctacttgctt ttatctattt tgggcttgat tgtggtctat tcgaccacca
     1621 gtgctatttt aattgaagaa ggcaagagcg ccttgcagtt ggttcgaaac caaggaatct
     1681 tttggattgt tagtttgata ctgattgcct taatttataa attgagacta gattttttga
     1741 gaaatgagcg actaatcatt ttagttatat taatagaaat gcttttattg ttcttggctc
     1801 gttttattgg tatttccgta aacggggcat acggttggat ttcggttgca ggaataacta
     1861 ttcagccagc tgagtactta aaaatcatta ttatttggta tttagctcac cgattctcca
     1921 aacagcaaga agaaatagct acttatgatt ttcaagtttt gactcaaaat caatggcttc
     1981 cccgtgcttt taatgattgg cgattcgttc tcctagttct gattggaagt ttgggaattt
     2041 tccctgattt aggaaatgcg actattttag tcttggtttc cttgattatg tatacagtta
     2101 gtggaatcgc ttatcgctgg ttttcaacca ttctggcgct cgtatctgcc gcttctgtct
     2161 ttgtcttgac cactatcagc ctaatcggtg ttgagacctt ttcaaaaatt ccagtatttg
     2221 gctatgtagc caagcgcttt agtgcctttt ttaatccttt tgccgatcgt gctgatgcag
     2281 gtcaccagtt agctaattct tattttgcca tggtcaatgg cggttggttt ggtctaggtc
     2341 ttggaaactc gattgaaaaa cgaggttatt tgccagaagc tcatacagac tttgtctttt
     2401 ctatcgtgat tgaagaattt ggctttgttg gtgccagtct tattttagct ctcttatttt
     2461 tcatgatttt gcggattatc ttggtcggta tccgagcgga gaatcctttc aatgccatgg
     2521 ttgcactcgg tgtcggaggg atgatgttgg ttcaggtatt tgtcaatatc ggagggattt
     2581 cgggcttgat tccatctaca ggagtaacct tccccttctt atcccagggt ggaaatagtc
     2641 ttctagtctt atcagtggca gtagcctttg tcttaaatat tgatgccagt gaaaaacgcg
     2701 ctaaattgta ccgagaattg gaaaatcaac caatgaacct tctgttgaag taggataaag
     2761 aaaggatagt ttatgtctct tcaaaaatta gaaaattata gtaataaaag tgttgtgcaa
     2821 gaagaagtct tgattctaac agaattactg gaagatatta ctaaaaatat gcttgcccca
     2881 gagacctttg aaaaaataat acagttgaaa gaattatcaa cgcaggaaga ttatcaaggt
     2941 ctaaaccgtc tagtgactag cttatcaaat gatgaaatgg tctatatttc acgctatttc
     3001 tctatcttgc ctcttttgat taatatttca gaggatgtgg atttagctta tgaaatcaat
     3061 catcaaaata atattgatca ggactattta ggtaaattat ctacaacgat taaattggta
     3121 gcagaaaagg aaaatgccgt tgagatccta gaacacttga atgttgtccc tgttttgaca
     3181 gcccatccaa cacaagtgca acgcaaaagt atgttggatt taacaaatca tattcatagt
     3241 cttttgcgta aataccgtga tgttaagttg gggttgatta ataaagataa atggtacaat
     3301 gatttgcgtc gttacatcga aattatcatg cagacagaca tgattcgtga gaaaaaatta
     3361 aaagtgacta acgaaatcac gaatgctatg gaatattata acagctcctt tttgaaagct
     3421 gtacctcatt tgacgacgga gtataagcgc ttagcgcaag cgcatggtct gaatttaaaa
     3481 caggctaaac caatcaccat gggtatgtgg ataggtggtg accgtgatgg aaatccattt
     3541 gttacagcaa agaccttgaa gcagtctgca ctcactcagt gtgaagtcat catgaactac
     3601 tatgataaaa agatttacca actttatcgt gaattttctc tttcaactag cattgtcaac
     3661 gtcagcaagc aagtcagaga aatggctcgt caatccaagg ataactcgat ttaccgcgaa
     3721 aaagagcttt accgtcgtgc cttgtttgat attcaatcaa aaattcaggc aactaaaacc
     3781 tatctgattg aggatgaaga agttgggact cgttatgaaa ccgccaatga tttctacaag
     3841 gatttgattg ccattcgaga ttctctacta gaaaataagg gtgagtcctt gatttcaggt
     3901 gattttgtgg aattattgca ggcagtagag atatttggtt tttacttagc gtcaattgat
     3961 atgcgacaag actctagcgt ctatgaagcc tgtgtggcag aactcttgaa atcagcagga
     4021 attcattctc gttatagcga gttgagcgaa gaagaaaagt gtgaccttct cttgaaagaa
     4081 ttagaagaag atccccgaat tctttctgcg actcacgcag aaaaatcaga attattagca
     4141 aaagaattag ctatttttaa gacggctcgt gttttgaaag ataagttggg agatgatgtc
     4201 atccgtcaga ccatcatttc acatgcaacc agcctttctg atatgctaga attagctatt
     4261 ctgttaaaag aagtaggact ggtggatacg gaaagggcgc gtgttcagat tgttcccctt
     4321 tttgaaacaa ttgaagactt ggatcattca gaggaaacaa tgagaaaata tctttctctt
     4381 agccttgcca aaaaatggat tgactcacga aataactacc aagaaatcat gcttggctac
     4441 tctgacagta ataaagatgg cggttacctg tcatcatgtt ggaccctcta caaggctcaa
     4501 caacaattga ctgctattgg agatgaattt ggcgttaagg ttaccttctt ccatggtcgt
     4561 ggtggtactg tcggtcgtgg tggtgggcca acctatgaag ccattacatc tcaaccgctc
     4621 aagtctatca aggatcgtat ccgcttgacg gagcagggtg aagtaattgg gaataaatac
     4681 ggtaacaaag acgccgctta ctataacctt gaaatgctag tatcggcagc tattaaccgt
     4741 atgattactc agaagaagag cgataccaat accccaaatc gttatgaagc cattatggat
     4801 caagtagtgg accgtagtta cgatatctac cgtgatttgg tctttggtaa tgagcatttc
     4861 tatgattatt tcttcgagtc aagtccaatc aaggctattt caagttttaa tattggttct
     4921 cgtccagccg ctcgtaagac tattactgaa atcggtggtt tgcgtgccat cccttgggta
     4981 ttctcatggt cacagagtcg tgttatgttc cctggatggt acggggttgg ttcaagcttc
     5041 aaggaattta tcaataaaaa tccagagaat attgctatct tacgagatat gtaccaaaat
     5101 tggcctttct tccaatcgct tctttcaaat gttgatatgg ttttgtcaaa atcaaatatg
     5161 aatattgctt ttgaatatgc taaactttgt gaagacgagc aagttaaggc catctatgag
     5221 actattttaa atgaatggca agttactaag aacgttatct tggctattga aggacatgac
     5281 gaactcttag ctgacaatcc atatctaaaa gctagtctgg attaccgtat gccttacttt
     5341 aatattctca actatattca gttagagttg attaaacgcc aacgtcgtgg agaattgtcc
     5401 agtgatcaag aacgattgat tcatatcacc atcaacggaa ttgcgacagg attgcgtaat
     5461 tcaggttgat aattttcaag agtgaatgct aaaagtgaat atcaaaaaaa ttctaataga
     5521 ctattgacaa gtagtttaaa aatgatataa tttaaccatt cagaaaagta atcatacaaa
     5581 ctttttagag agtctgtggt agctgaaaac agataagtgg cgatgatgaa aattgggctg
     5641 aatgctattt agaatttgaa attataaaaa ttcggtaagc acaccttaca gtgcatctcg
     5701 ttattgcgag actgagcgat agggaaattc cctataattg aggtggtacc gcgcatcgac
     5761 gtcctcacac aagttttttg tgtgaggatt tttttgatgg aggttagtat ggaaagaaaa
     5821 cgatggcgtc gcttgtttag ataagtgaaa tatgttaaag gaaataaaaa ggagaaacag
     5881 aatgaaaaat aaacgtttaa ttggaattat tgctgcatta gcagtcttag tagcaggaaa
     5941 cttgatttat tcttcaatga ataaatcaga agttcagaat aataaggatg agaagaaaat
     6001 aaccaagatt ggtgtgcttc aatttgtgag ccatccatcc cttgatttga tttataaagg
     6061 gatccaagat ggacttgcag aagaaggata taaagatgat caagttaaaa ttgattttat
     6121 gaactcagaa ggtgaccaaa gtaaggttgc gacaatgagt aaacaattgg ttgcaaatgg
     6181 aaatgacctt gtggttggta tcgcaacacc agcagcccaa gggttggcta gtgcaacaaa
     6241 agacctaccg gttatcatgg ccgctattac agacccaatt ggtgctaact tggttaaaga
     6301 tttgaaaaaa ccaggtggca acgttacagg ggtatctgac cacaatccag ctcaacaaca
     6361 agttgaactc atcaaggctc tgacaccgaa tgtgaaaaca atcggagctc tttactcaag
     6421 tagcgaagac aattcaaaaa cacaggtcga agaatttaag gcttatgctg aaaaagcagg
     6481 tctgacagtg gaaacatttg cagttccttc aacaaatgaa attgcctcaa ctgtcactgt
     6541 tatgactagc aaggtagatg ctatttgggt tccaattgat aacaccattg catcaggatt
     6601 tccaacggtt gtctctagca atcaaagttc taagaaacca atttatccca gtgcgacagc
     6661 tatggtagaa gtaggtggtt tggcatcagt tgtaattgac caacatgacc ttggtgtggc
     6721 aacaggtaaa atgattgtgc aagtcttgaa aggtgcaaaa ccagccgata ccccagtcaa
     6781 tgtcttttca attggtaagt cagtcatcaa taaaaaaata gcacaagaac taggtattac
     6841 tattcctgag tctgttctca aagaagcagg acaagtcatc gaataaataa ggtagggagg
     6901 aggagactct tcccattttt taaaaagatg aatatagaaa ggattaaata aaatatgatt
     6961 gtttccatta tttctcaagg atttgtctgg gctattctag gtctgggaat ctttatgaca
     7021 tttaggattt taaactttcc agatatgacg acagaaggtt ccttccctct tgggggagct
     7081 gttgctgtca ctttgataac caaaggcgtg aacccatttt tagcgacact tgttgctgta
     7141 ggagcaggtt gtttggctgg aatggcagca ggccttcttt atacaaaagg gaagatccca
     7201 accttgctct cagggatttt ggtgatgact tcttgtcact caatcatgct cttgattatg
     7261 ggacgtgcga atttaggcct gcttggaacc aagcaaattc aggatgtttt gccttttgat
     7321 tcggatttga atcaactctt gacaggtctc atctttgtga gcattgttat tgctctcatg
     7381 ctctttttct tggacactaa actcggacaa gcctatattg ctacagggga taatcctgat
     7441 atggctagaa gtttcgggat tcatactgga cgcatggagc tcatgggctt ggtcttatca
     7501 aatggtgtga ttgcccttgc aggtgccctc attgctcagc aagaaggtta tgccgatgtg
     7561 tctcgaggga tcggggttat cgttgtgggg cttgcaagtt tgattattgg agaagttatt
     7621 ttcaagagtt tgagcttggc agagcgtttg gttactatcg ttgtaggttc tatcgcttat
     7681 caatttttag tgtgggcagt tatcgcactt ggctttaata caagttacct tcgtttatac
     7741 agtgccttga ttttagcagt ctgcctcatg attccaacat ttaagcaaac aatcttgaaa
     7801 ggagccaagt taagcaaatg acagcaattg tagaattaaa aaatgcaacc aaaatcgtta
     7861 aaaatggctt tgatgaagaa aagattattt taaatgatgt ttccttagaa atttttgaac
     7921 gggactttat cacgattttg ggcggaaatg gtgctggaaa atcaaccctc tttaacacta
     7981 tagcagggac cttatcacta actagtggaa ctatccgtat tttaggtgaa gatctcacta
     8041 agttttcacc cgagaagcgt gccaagtacc tgtctcgtgt cttccaagat ccaaagatgg
     8101 ggacagctcc ccgtatgacg gtcgctgaaa atcttttaat cgccaagttt cgtggtgaaa
     8161 agcgtggatt gttaccaaga cgcttgacta gctataagga tgaatttcag gcaaccattg
     8221 aaaaagtagg aaatggtctt gagaaacact tgaatacacc gattgagttc ttatcaggtg
     8281 gacaaagaca ggctttgagt ctcttgatgg caaccttgaa gcgacctgaa ttactcttgt
     8341 tagacgagca tactgctgcc ctggatccaa agactagtgt tgctttgatg gaattgacag
     8401 atgaatttgt taagaaagat cagctaacag cccttatgat tactcatcat atggaagatg
     8461 ctctcaaata cggcaatcgc ttgattgtca tgaaagaagg acggattatc caagatttaa
     8521 accaagaaga aaaagcaaaa atgaaaatct ctgattatta tcaactcttt gaataaagta
     8581 aggaaaatga gtgaaatggt ttactttttt tctgaaataa agtatactat ataaagtaaa
     8641 ctatgataac atggaggtat tgtgtatggt taacaaacaa gtcattgaag aaatcaaaaa
     8701 caatgccaac attgtggaag tcataggaga tgtgatttct ttacaaaagg caggacggaa
     8761 ctatctaggg ctctgtcctt ttcatggtga aaaaacacct tctttcaacg ttgtagagga
     8821 taagcagttt tatcactgtt tcggttgtgg tcgctcaggt gatgtcttta agttcataga
     8881 ggagtaccaa ggggttccct ttatagaggc agtccaaatc ttaggtcagc gtgttggaat
     8941 tgaggttgaa aaaccgcttt atagtgagca aaagtcagcc tcacctcacc aagatcttta
     9001 tgatatgcac gaagatgcag ccaaatttta ccatgctatt ctcatgacaa cgactatggg
     9061 cgaagaggcc agaaattacc tttatcagcg gggtttgaca gatgaagtgc ttaaacattt
     9121 ttggattggt ttagcacctc cagaacgaaa ctatctctat caacgtttgt ctgatcagta
     9181 tcgtgaagag gatttactgg attcaggcct gttttatctc tcggatgcca atcaatttgt
     9241 agacaccttt cacaatcgca ttatgtttcc cctgacaaat gaccaaggaa aggtcattgc
     9301 cttctcaggt cgtatctggc aaaaaacgga ttcacaaact tctaagtata aaaacagccg
     9361 atcgactgca atttttaaca aaagttacga attatatcat atggataggg caaaaagatc
     9421 ttctggaaaa gctagtgaga tttacctgat ggaaggattc atggatgtta ttgcagccta
     9481 tcgggctgga atcgaaaatg ctgtggcgtc gatgggaacg gccttgagtc gagagcatgt
     9541 tgagcatctg aaaaggttaa ccaagaaatt ggttcttgtt tacgatggag ataaggctgg
     9601 gcaagccgcg acattgaaag cattggatga aattggtgat atgcctgtgc aaatcgttag
     9661 catgcctgat aacttggatc ctgatgagta tctacaaaaa aatggtccag aagacttggc
     9721 ctatctatta acgaaaactc gtattagtcc gattgagttc tacattcatc agtacaaacc
     9781 tgaaaacagt gaaaatctgc aggctcagat tgagtttctt gaaaaaatag ctcccttgat
     9841 tgttcaagaa aagtccatcg ctgctcaaaa cagctatatt catattttag ctgacagtct
     9901 ggcgtccttt gattataccc agattgagca gattgttaat gagagtcgtc aggtgcaaag
     9961 gcagaatcgc atggaaagaa tttccagacc gacgccaatc accatgcctg tcaccaagca
    10021 gttatcggct attatgaggg cagaagccca tctactctat cggatgatgg aatcccctct
    10081 tgttttgaac gattaccgtt tgcgagaaga ctttgcattt gctacacctg aatttcaggt
    10141 cttatatgac ttgcttggcc agtatggaaa tcttcctcca gaagttttag cagagcagac
    10201 agaggaagtt gaaagagctt ggtaccaagt tttagctcag gatttgcctg ctgagatatc
    10261 gccgcaggaa cttagtgaag tagagatgac tcgaaacaag gctctcttga atcaggacaa
    10321 tatgagaatc aaaaagaagg tgcaggaagc tagccatgta ggagatacag atacagccct
    10381 agaagaattg gaacgtttaa tttcccaaaa gagaagaatg gagtaataat ggcaacaaaa
    10441 caaaaagaag taacaacatt tgacgtacag gtagcagaat ttatccgtaa tcataagcaa
    10501 aaagggacag caacagatga tgaaatcaat gcaagtctgg ttattccttt taccttggac
    10561 gctgatggga ttgaagatct cttgcaacgg attcaggatg cagggatttc tatcacagat
    10621 aacgaaggaa atccaagtgc gcgtgttctt agcaatgaag aagaaccaga actcagcgat
    10681 gaggacttga ttggatcaac ttctgctaag gtcaatgacc ctgtccgtat gtacttgaaa
    10741 gaaatagggg tcgttcctct cttgaccaat gaagaggaga aagagttggc actggctgtt
    10801 gaagctggcg atatcgaagc caaacaacgt cttgcggaag ccaatcttcg tttggttgtt
    10861 tccattgcca aacgctatgt cggtcgtggc atgcagttcc ttgacttgat tcaagaagga
    10921 aatatgggct tgatgaaggc ggttgacaag tttgactatt ctaaagggtt caagttttca
    10981 acttatgcaa cttggtggat tcgtcaggct atcactcgtg ctattgcgga ccaagctcgt
    11041 accatccgta tcccagttca catggttgaa actatcaata aattggttcg tgaacagcgg
    11101 aatctccttc aagaattggg gcaagatccg acaccagaac agattgctga acgaatggat
    11161 atgacacctg ataaggttcg tgaaatcttg aagattgccc aagaaccagt atctcttgaa
    11221 actcctatcg gtgaagagga cgatagccac cttggagact ttatcgaaga tgaagtgatt
    11281 gaaaatccag tggattatac gactcgtatc gtcttgcgtg agcaattgga tgaaatctta
    11341 gatactctta cagaccgtga agaaaatgtt ctgcgtctac gttttggact agatgatgga
    11401 aaaatgcgca cacttgaaga tgtggggaaa gtctttaacg taactcgtga gcgtatccgt
    11461 cagattgaag caaaggcttt gagaaaacta cgccaaccaa gccgcagcaa accgcttcgt
    11521 gattttattg aagactaaga gtgaggaaaa tatggcttat acagaagagc aaattgaaaa
    11581 catcaaaaca cggattttaa cagccttgga agaagtcatc gaccctgagt tgggaatcga
    11641 tattgtcaat cttggtttga tctatgagat tcgttttgac ggtgacacag ggcaaacaga
    11701 gattgacatg actttgacaa ctatgggttg tcccttagca gaccttttga cagaccagat
    11761 ttatgatgct atgatagagg ttccagaagt aacggatact gaggttaaat tagtctggta
    11821 tccagcctgg acagttgaaa aaatgagtcg ctatgcccgc attgccctag gcattaagta
    11881 aacatataaa agcatgtgag agactgttgg aaaagcgagg aaatttcccc tcttttcctc
    11941 tagtctctcc tttcttttgc tgattttatt caaagaaaat gatataatag tagttatgga
    12001 gaaaaagaaa ttacgcatca atatgttgag ttcaagtgag aaagtagcag gacagggagt
    12061 ttcaggtgct taccgtgaat tagttcgtct tcttcaccgt gctgccaagg accaattgat
    12121 tgttacagaa aatcttccga tcgaggcaga tgtgactcac tttcatacga ttgattttcc
    12181 ctattattta tcaaccttcc aaaagaaacg ctcagggaga aagattggct atgtgcattt
    12241 cttgccagct acacttgagg gaagtttgaa aattccattt ttcttaaagg aaattgtgaa
    12301 acgctatgta ttttcttttt acaaccggat ggagcacttg gttgtggtca atcctatgtt
    12361 tattgaggat ttggtagcag ctggtattcc acgtgaaaaa gtgacctata ttcctaactt
    12421 tgtcaacaag gaaaaatggc atcctctacc acaagaagag gtagttagac tgcgcacaga
    12481 tcttggtctt agtgacaatc agtttatcgt agtaggtgct gggcaagttc agaaacgtaa
    12541 agggattgat gactttatcc gtctggctga ggaattgcct cagattacct ttatctgggc
    12601 tggtggcttc tcttttggtg gtatgacaga tggttatgaa cactataaga aaattatgga
    12661 aaatccccct aaaaatttga tttttccagg cattgtatcg ccagagcgga tgcgcgaatt
    12721 gtatgctcta gcggatcttt tcttgttgcc tagttacaat gagctctttc ctatgactat
    12781 tttagaagct gcgagttgtg aggctcctat tatgttgcgt gatttagatc tctataaggt
    12841 gattttggag ggaaattatc gggcgacagc gggtagagaa gagatgaaag aggctatttt
    12901 ggaatatcaa gcaaatcctg ctgccttaaa agatctcaaa gaaaaggcta agaatatttc
    12961 cagagagtat tctgaagagc atctgttaca aatctggttg gacttttatg agaaacaagc
    13021 cgctttaggg agaaagtaaa aagtgaggta atctatgcga attggtttat ttacagatac
    13081 ctattttcct caggtttctg gtgttgcgac cagtattcga accttgaaaa cagaacttga
    13141 aaagcaggga catgctgttt ttatctttac gacgacagat aaggatgtca atcgctacga
    13201 agattggcaa attatccgca ttccaagtgt tcctttcttt gcttttaagg atcgtcgctt
    13261 tgcctaccga ggttttagca aggcacttga aattgctaaa cagtatcagc tagatattat
    13321 ccatactcag acagaatttt ctcttggcct gttggggatt tggattgcgc gtgaattgaa
    13381 aattccagtc atccatacct atcacaccca gtatgaagac tatgtccatt atattgctaa
    13441 ggggatgttg atccggccga gtatggtcaa gtatctggtt agaggtttcc tgcatgatgt
    13501 ggatggggtt atttgtccta gtgagattgt ccgtgacttg ctatctgatt ataaggtcaa
    13561 ggttgaaaaa cgggtcattc ctactgggat tgaattagcc aagtttgagc gtccggaaat
    13621 caagcaggaa aatttgaaag aactgcgtag taaactaggg attcaagatg gtgaaaagac
    13681 gttgcttagt ctttcgagaa tctcctatga aaaaaatatt caagcagttt tagcagcctt
    13741 ttctgatgtt ctgaaagagg aagacaaggt taaactggta gtagctgggg atggccctta
    13801 tctgaatgac ctcaaagagc aagctcagaa cctagagatt caagactcag tcatctttac
    13861 agggatgatt gctcctagtg agacggctct ttactataaa gcggcggatt tcttcatttc
    13921 ggcatcgaca agcgaaacgc aaggtttgac ctacttggaa agcttagcca gtggaacacc
    13981 tgtcattgct cacggaaatc cttatttgaa caacctcatc agtgataaaa tgtttggaac
    14041 cttgtactat ggagaacatg atttggctgg tgctattttg gaagccctga ttgcaacacc
    14101 agacgtgaac gagcatacct tatcagagaa attgtatgag atttcagctg agaactttgg
    14161 gaaacgagtg catgagtttt atctggatgc cattatttca aataacttcc agaaagattt
    14221 ggctaaagat gatacggtca gtcagcgtat ctttaagaca gttttgtatc ttcagcaaca
    14281 ggtggttgct gtacctgtaa aaggatctag acgcatgttg aaggcttcaa aaacacagtt
    14341 gatcagtatg agagactatt ggaaagacca tgaagaatag aaagaggaac agctatgaaa
    14401 aaaacaatta atgagaagcg gtcgtgataa aaagattgcg ggtgtttgtg ctggggtggc
    14461 ccattatctg gatatggatc cgactatcgt tcaagtcatt tggggtgttc ttacttgctg
    14521 ttacggagct ggaattgtag cttacattat tttatggatt atcgcaccag tagcaactga
    14581 ctattgaaaa ctagctcaat tcatgctaag ataatagaaa atagaatgta acgaaggaga
    14641 gaaaaatggc atttggagat aatggaaatc gtaaaaaaac tatgtttgag aaaataacct
    14701 tgtttatcgt gattatcatg ctagtagcaa gtttattggg aatttttgca actgcaattg
    14761 gtgccctcag taatctataa aatagattca agaaaattta gtgactggga tttcccagcc
    14821 cttttttaaa gtgagaagaa ataatgagta tgtttttaga tacagctaag attaaggtca
    14881 aggctggtaa tggtggcgat ggtatggttg cctttcgtcg tgaaaaatat gtccctaatg
    14941 gaggcccttg gggtggtgat ggtggtcgtg gaggcaatgt ggtcttcgtt gtagacgaag
    15001 gactacgtac cttgatggat ttccgctaca atcgtcattt caaggctgat tctggtgaaa
    15061 aagggatgac caaagggatg catggtcgtg gtgctgagga ccttagagtt cgagtaccac
    15121 aaggtacgac tgttcgtgat gcggagactg gcaaggtttt aacagatttg attgaacatg
    15181 ggcaagaatt tatcgttgcc cacggtggtc gtggtggacg tggaaatatt cgtttcgcga
    15241 caccaaaaaa tcctgcaccg gaaatctctg aaaatggaga accaggtcag gaacgtgagt
    15301 tacaattgga actaaaaatc ttggcagatg tcggtttagt aggattccca tctgtaggga
    15361 agtcaacact tttaagtgtt attacctcag ctaagcctaa aattggtgcc taccacttta
    15421 ccactattgt accaaattta ggtatggttc gcacccaatc aggtgaatcc tttgcagtag
    15481 ccgacttgcc aggtttgatt gaaggggcta gtcaaggtgt tggtttggga actcagttcc
    15541 tccgtcatat cgagcgtaca cgtgttatcc ttcacatcat tgatatgtca gctagcgaag
    15601 gccgtgatcc atatgaggat tacctagcta tcaataaaga gctggagtct tacaatcttc
    15661 gcctcatgga gcgtccacag attattgtag ctaataagat ggacatgcct gagagtcagg
    15721 aaaatcttga agaatttaag aaaaaattgg ctgaaaatta tgatgaattt gaagagttac
    15781 cagctatctt cccaatttct ggattgacca agcaaggtct ggcaacactt ttagatgcta
    15841 cagctgaatt gttagacaag acaccagaat ttttgctcta cgacgagtcc gatatggaag
    15901 aagaagctta ctatggattt gacgaagaag aaaaagcctt tgaaattagt cgtgatgacg
    15961 atgcgacatg ggtactttct ggtgaaaaac tcatgaaact ctttaatatg accaactttg
    16021 atcgtgatga atctgtcatg aaatttgccc gtcagcttcg tggtatgggg gttgatgaag
    16081 cccttcgtgc gcgtggagct aaagatgggg atttggtccg cattggtaaa tttgagtttg
    16141 aatttgtaga ctaggagact ggtatgggag ataaaccgat atctttccga gatgcggatg
    16201 gtaattttgt ttccgccgca gacgtttgga atgaaaagaa attggaagaa ctatttaatc
    16261 gtctcaatcc aaatcgtgcc ttgagattgg cacgaactaa aaaggaaaat ccatctcagt
    16321 aaagaagcta aaaaatcccg tgcctcatca gacacgggat tttgtggtac gacgggcatg
    16381 tatagcgaac tgaatctgga atagcacagc atatcttcta aaatatagta aaatgaaatg
    16441 agaacaggac aaatcgatca ggacagtaaa atcga
//
LOCUS       DAVKJU010000047        16204 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVKJU010000047 DAVKJU010000000
VERSION     DAVKJU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.047"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            247..1368
                     /gene="mnmA"
                     /locus_tag="VW191_001454"
     CDS             247..1368
                     /gene="mnmA"
                     /locus_tag="VW191_001454"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU9624205.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1509..1963
                     /locus_tag="VW191_001455"
                     /pseudo
     CDS             1509..1963
                     /locus_tag="VW191_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            1973..3886
                     /gene="mnmG"
                     /locus_tag="VW191_001456"
     CDS             1973..3886
                     /gene="mnmG"
                     /locus_tag="VW191_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU9624206.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELTETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4075..4164
                     /locus_tag="VW191_001457"
                     /pseudo
     CDS             4075..4164
                     /locus_tag="VW191_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4311..5990)
                     /locus_tag="VW191_001458"
     CDS             complement(4311..5990)
                     /locus_tag="VW191_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU9624207.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKAP
                     KGTFIEPNEIKDYPAGEILILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(5992..6225)
                     /locus_tag="VW191_001459"
     CDS             complement(5992..6225)
                     /locus_tag="VW191_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEU9624208.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6607..6828
                     /locus_tag="VW191_001460"
     CDS             6607..6828
                     /locus_tag="VW191_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624209.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7041..7193)
                     /gene="cibB"
                     /locus_tag="VW191_001461"
     CDS             complement(7041..7193)
                     /gene="cibB"
                     /locus_tag="VW191_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEU9624210.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7195..7380)
                     /gene="cibA"
                     /locus_tag="VW191_001462"
     CDS             complement(7195..7380)
                     /gene="cibA"
                     /locus_tag="VW191_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEU9624211.1"
                     /translation="MTNFDILNNQFLSLSENELSDIDGGLAPLVIFGVAVSWKVIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7558..8241
                     /gene="tsaB"
                     /locus_tag="VW191_001463"
     CDS             7558..8241
                     /gene="tsaB"
                     /locus_tag="VW191_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU9624212.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNYSITLMPAID
                     FLMASLDWTPKDLGRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8238..8675
                     /gene="rimI"
                     /locus_tag="VW191_001464"
     CDS             8238..8675
                     /gene="rimI"
                     /locus_tag="VW191_001464"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU9624213.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMIAVYPVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREVDEG"
     gene            8665..9675
                     /gene="tsaD"
                     /locus_tag="VW191_001465"
     CDS             8665..9675
                     /gene="tsaD"
                     /locus_tag="VW191_001465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU9624214.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9715..11021)
                     /locus_tag="VW191_001466"
                     /pseudo
     CDS             complement(9715..11021)
                     /locus_tag="VW191_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11413..11493)
                     /locus_tag="VW191_001467"
     CDS             complement(11413..11493)
                     /locus_tag="VW191_001467"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9624215.1"
                     /translation="MLPLPTYSPEYNPIEKIWAYIKNISE"
     gene            complement(11727..12053)
                     /locus_tag="VW191_001468"
     CDS             complement(11727..12053)
                     /locus_tag="VW191_001468"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624216.1"
                     /translation="MEKVDRDKLKIYIETHPDAYLTEIAAEFNCPPTTIHYALKAMGY
                     SLKKSRTYCEQDPEKVNRFLKELNHLSYLTPIYIYETGVETYFYLEYDRALSRQLVSL
                     EEDIII"
     gene            12290..13432
                     /locus_tag="VW191_001469"
     CDS             12290..13432
                     /locus_tag="VW191_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU9624217.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIQLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCIPNN
                     KKIICYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKCRD
                     NFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEH
                     RVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            13456..14331
                     /locus_tag="VW191_001470"
     CDS             13456..14331
                     /locus_tag="VW191_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9624218.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGSKNSRGKYISFLDDDDE
                     YMSDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNSLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGCQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLF"
     gene            14418..16034
                     /locus_tag="VW191_001471"
     CDS             14418..16034
                     /locus_tag="VW191_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624219.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
BASE COUNT         4904 a         2874 c         3619 g         4807 t
ORIGIN      
        1 gaaaaataga ttagagaaaa tgggttgttt tatttattat agttatttga atgaagataa
       61 gaagaaggta tactcacatc attcacataa tctgtatgtt gactataagt tttaaaaaac
      121 aatttttaag ctcttccttg tcttctccaa ccaagcgtgt tataatgaat actgctcaag
      181 cgaccttcaa tcgtgaagca cacacgacct tcaatcgtga ataaacgaat agatgggaga
      241 cttaccatga gtgataactc taaaacacgt gttgtcgtgg ggatgagtgg tggtgttgat
      301 tcgtcggtga cggctctttt gctcaaggag cagggctacg atgtgatcgg tatcttcatg
      361 aagaactggg atgacacaga tgaaaacggc gtctgtacgg cgaccgaaga ttacaaggat
      421 gtggttgcgg tggcagacca gattggcatt ccctactact ctgtcaattt tgaaaaagag
      481 tactgggacc gcgtttttga gtatttccta gcggaatacc gtgcagggcg cacgccaaat
      541 ccggacgtta tgtgcaacaa ggaaatcaag ttcaaggcct ttttggacta tgccatgacc
      601 ttgggggcag actatgtagc gactgggcat tatgctcgag tggcgcgtga tgaggacggc
      661 actgttcaca tgcttcgtgg cgtggacaat ggcaaggacc agacctattt cctcagccaa
      721 ctttcgcaag aacaacttca aaaaaccatg ttcccactag gacatttgga aaagcctgaa
      781 gttcgaaaac tagcagaaga agcaggcctt tcgactgcta agaagaaaga ctcgacaggg
      841 atttgcttta tcggagaaaa gaactttaaa aacttcctca gcaactacct gccagctcag
      901 cctggtcgca tgatgactgt ggatggtcgc gatatgggcg agcatgcagg tcttatgtac
      961 tatacaatcg gtcagcgtgg cggactcggt atcggtgggc aacacggcgg tgacaatgcc
     1021 ccttggttcg ttgtcggaaa agatctaagc aagaatattc tctatgtagg acaaggattc
     1081 taccatgatt cgctcatgtc aactagccta gaagccagtc aagtccactt tactcgtgaa
     1141 atgccagaag agtttacgct agaatgtacg gctaaattcc gttaccgtca gcctgactct
     1201 aaggtgaccg tccatgtcaa aggagacaag gcagaggtca tctttgcgga accacaacgc
     1261 gcgattacac caggacaggc agttgtcttt tacgatggcg aagagtgtct aggtggtggt
     1321 ttgattgaca atgcttaccg cgatggacaa gtttgtcagt acatttagat tgacaaattt
     1381 tctcaatttg ctacaataat aacagcaata gaaatgatgg tcaaagctca tggatgttgc
     1441 aggctttttt gtcctgcact tctttgtagt tttgactgtt tttgtgtcgt ttaagggaaa
     1501 ggacaagaat gactcaacaa gactttcgga caaaagtaga caatacggtt tttggtgttc
     1561 gggcgacagc tttgattctt caaaatcgca agcttctagt caccaaagac aagggcaagt
     1621 attacattat cggcggtgcg attcaagtta acgaaaaaac ggaagacgcg gtagtccgtg
     1681 aagtgaagga agaactgggt gttaaatctc aagctgggca gctagctttt gtggttgaaa
     1741 atgttttgaa gtggacggcg tttcctatca caatatcgag tttcattatc tggtggattt
     1801 gcttgaagat gccccgttga ccatgtagga agatgagaaa aggcagccct gtgagtggat
     1861 tgacttggat aagctccaga atatccagct agttccagcc tttttaaaaa cagccctacc
     1921 agattgggaa ggccaactaa gacacattca tcttgaggaa taggagagaa acatgactta
     1981 tcattttact gaagaatacg atattattgt aattggtgcg ggacacgctg gggttgaggc
     2041 ttccttggcc gctagccgta tgggctgtaa ggtcctgctt gcgaccatca atattgaaat
     2101 gctggctttc atgccttgta atccctctat cggtggttct gccaagggga ttgtcgtgcg
     2161 tgaagtcgat gccctcggtg gcgagatggc caagactatt gacaagactt acatccagat
     2221 gaagatgctc aacacaggaa aggggccagc tgtccgtgct cttcgtgcgc aggctgacaa
     2281 ggaactttac tctaaggaga tgcgcaagac agttgaaaat caagaaaatc tgacccttcg
     2341 tcaaaccatg attgatgaga ttttggtgga agatggcaag gttgtcggtg tgcgtacagc
     2401 cacccatcaa gaatatgctg ctaaggctgt tattgtgacg acagggactg ctctccgtgg
     2461 ggaaattatc atcggagacc tcaagtactc atcaggtcct aaccacagcc tagcttctat
     2521 taaccttgct gataatctca aggaactggg tctcgaaatc ggtcgtttca agacaggaac
     2581 ccctccacgt gtcaaggcct cttctatcaa ctacgacgtg acagaaatcc aaccaggaga
     2641 tgaagcgcct aatcacttct catacacttc acgtgatgag gattatgtca aggaccaagt
     2701 gccatgctgg ttgacctata ccaatggtac cagtcatgag attatccaaa acaacctcca
     2761 ccgtgcgcct atgtttacag gtgtggtcaa gggagtgggg cctcgttact gtccgtcgat
     2821 tgaagacaag attgtgcgct ttgcggacaa ggaacgtcac caactcttcc ttgagccaga
     2881 aggacgtaat acagaggaag tctatgtgca aggcctctca accagtctgc ctgaggatgt
     2941 ccagcgtgac ttggttcatt ccatcaaagg tttggaaaat gcagagatga tgcggacagg
     3001 ttatgctatt gagtatgata tggtcttgcc tcatcagttg cgtgcgactc tggaaaccaa
     3061 gaaaatctca ggtctcttca ctgctggtca gacaaatgga acgtcaggtt acgaagaggc
     3121 agcaggccaa gggattatcg cgggtatcaa tgcggctctg aaaatccaag gtaaacctga
     3181 gttgattcta aaacgaagtg acggttatat cggggtgatg atcgacgact tggtgaccaa
     3241 gggaaccatt gaaccttacc gtctcttgac cagtcgtgct gaataccgtc tcattcttcg
     3301 tcatgacaat gctgatatgc gtttgacaga gattggccgc gagattggcc ttgtggatga
     3361 tgaacgctgg gctcgttttg aaatcaagaa aaatcaattt gataatgaga tgaaacgcct
     3421 agacagtatc aaactcaagc cagtcaagga aaccaatgct aaggttgagg aaatgggctt
     3481 caagccgttg acagatgcgg tgacagccaa ggaattcctt cgccgtccag aagtttctta
     3541 ccaagatgtg gtggccttca tcggaccagc tgcagaagac ttggatgaca agattatcga
     3601 attgactgaa acagaaatca agtacgaagg ctatatttcc aaagctatgg atcaggttgc
     3661 caagatgaaa cgcatggaag aaaaacgcat tccagccaat atcgactggg atgacatcga
     3721 ttctattgcg acagaagctc gtcagaagtt caaactcatc aatccagaaa ccatcggcca
     3781 agccagccgt atttcgggag taaacccagc agatatttct attttgatgg tgtatctgga
     3841 aggtaaaaat cgtagtattt ctaaaactct tcaaaaatca aaatgatacg tcgtcggctt
     3901 cttacgaatg agttcaaagc ttggctttga ttcatctcca acctcccata gttccccgaa
     3961 ctatgggagc taactcaagt tatgcatttt cctagccttc tggtctgatt ttcattgagt
     4021 tttaatctca aaagaaagca tagagaaaaa cagctccgaa gacggggctg ttttgttgac
     4081 ttgtactcaa tgaaaatcaa agagcaaact aggaagctag ccgtaggttg ctcaaagcac
     4141 tgctttgagg ttgtagatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg
     4201 gatagaactg acgaagtcag ctcaaaacac cgttttgagg ttgtggatag aactgacgaa
     4261 gtcagtaacc atatatacgg caaggcgaag ctgacgtggt ttgaagagta ttattcttca
     4321 tctggtgtga ggatcatcgg gatgatgatc ggttcacgtt cggtattttc atagaggaag
     4381 gggcgaatag cgttgacaat ggcaccattg acagattgca cgctagcatc cttatttttc
     4441 agtgcgatac gaatggcatt gaagaggata cgctggcttt ggcgaatcaa gtcgccagac
     4501 tctctcatgt agacaaagcc tcggctgagg atatctggac cagatagaat catctgcgat
     4561 ttgaagtcaa cagttgcgac tgccagaacg acaccgtctt cagatagatc gcgacgatct
     4621 ttgaggacag ctgcgccaat ttcaccgata cgatttccat cgacatagat atcttgggcg
     4681 ttgaaatgac ctgcgatacg agctgagtca gcagtaaggg caagcacatc gccattgctc
     4741 atgataaaga tattgtcctt ctcaacacca gtatccactg ctagtccagc gtggactttt
     4801 tgcatgcggt attcaccgtg gacaggcatg aagtattttg gcttaatcaa gcagagcatg
     4861 agtttttgct cttgctgacc accgtgtcca gatgtatgga tattgttcac tttaccgtgg
     4921 ataacttcga caccagcttc agaaatgatg ttaatcagct tgttgacgct agtagtgttt
     4981 ccagggatgg gactagaaga gaagataacg gtatcacctg gttgtaattg tacttgacgg
     5041 tgggttccgt tggcgatacg agagagggct gccatgggct cgccctgact acctgtacag
     5101 aggatcagaa tctcacccgc aggatagtct ttgatttcat ttggttcgat aaaggttccc
     5161 ttaggcgcct tgatgtaacc aagctcgatt ccgttgacaa tggccttttc catagagcga
     5221 ccaaagaccg caatcttacg tccagtctta acagcagctt ctgttgcttg ctggagacgg
     5281 aagatatttg aggcgaagga tgcaaagatg atacgtccct cgataccttg gataatcttc
     5341 atgatcgact ggccgacaac cttttcagag tttgtaaagg ttggaacctc agcattggtc
     5401 gagtcagaga ggagacagag aacaccctct tcacctaggg ctgccatacg gtgcaagtct
     5461 gcaggttcac caactggagt aaagtcgaac ttgaagtcac ccgtacagac gattttccct
     5521 tgaggagtat ggatgacaat ccccaaaggt tctggaatag agtgagtcgt tctaaagaaa
     5581 gttgccttga gatttttaaa ggtcaactcg gtgttgtggt tgatttcgta aagtttagcg
     5641 ttgcgcaaga ggccgtgttc ttcgagtttt ccacgaatca aagccagcgc aagcggtcca
     5701 gcgtagatcg ggacatttgc ttgcttgagc aggaatggaa tcccaccgat gtggtcctcg
     5761 tgtccgtgcg taatcaaaac agccttgacg cggtcgatat tgtccacgat gtaagagtaa
     5821 tcaggaatga catagtcgat accaagcaag tcatcttctg ggaatttaat cccagcatcg
     5881 acgatgataa tctcatcttg gtattcaatt ccgtaagtgt ttttcccgat ttctcccaga
     5941 ccaccgatgg caaaaacgcc aacttcttca ggtttaagag tataggccat attagaactc
     6001 cgtgatttcg aaggcgccag tttctttttc gtaatctagc aatttgtcag acaagagttc
     6061 gatatactcg atattgtact ctgggcgatt ttcttcgaca agttggcgag cagtgatacg
     6121 gccctcaagt tctgagctgg catcgatgtc taggtaaagc gcgcgtgttg tttcacggcg
     6181 tgggctacgt tcttttgttt cttgataaaa aactttgtaa atcatatagt tcctttctat
     6241 ttacaggtca gcttattcca aacttttagc agatatttgc ttgatttcat tccattttgt
     6301 gtctagattg accttgattc gttacaatta tttcaattat accacaaaat gaaaaattag
     6361 taaaagacgg aaacgagaaa gttaggggac gatttcttcg gtaagcgttt gcaaaaataa
     6421 agaaaatgtg ttatgttgat aacggaagtg aagtttatta cttgtcataa ttctatatag
     6481 attttaagtc aaggaggttg ccctatgtat aacttatttg cttttgctat cggagttcgt
     6541 ttgctagctt ttatcgtttt tgttttgcgt ttatggagga aatcttctat ttgccaaact
     6601 ggaacgatga caaaccaagt tcttgaggaa aattaccttt gtgactctct actcgctttt
     6661 tctcctcctc gtgacctatg tcgtttggtt tgtattttac tttggattaa atgcttagat
     6721 gccctgctta tgctgggttt ttttgtttgg ggatgtagag atagtagatt tacgacacga
     6781 aaattccacc ccatctatca agatgaggtg gtctttgcat ttttctaaaa tgatatgaca
     6841 atggaatttt caaagcttat tatttttatg ggagtcattg ttctgttcaa aaatagaatt
     6901 gaagcataaa aataaaacaa tacaaacaga taaatcgaat ggtaaaggga tagtagtaat
     6961 gttcaaatca ttgagaatgc tgagcaaact gtaagagatg aagacaataa aatttatcct
     7021 tctaaaaatg tgtttcttat ctagcatgac ttaccaaact ttttacgaag gtcttttcca
     7081 atggtataac ctgccataaa taaagcgata ccaacaccta caccaaagcc acccttgatt
     7141 tcttgcaatt ccttattaga aatttcacga tagttgttca aatctttcat catattaatc
     7201 tcctcctaaa aaataaccag ctgccaaacc agaacctata agtgctgttc cacctgcaat
     7261 aaccttccaa gatactgcta ctccaaagat aaccaaggga gcgagaccgc catcaatatc
     7321 tgataattca ttttcagata aggataaaaa ttgattgtta agaatgtcaa aatttgtcat
     7381 tgaactttcc tccttttttc ttgttcactt ttatattcgg aaaagaaaag gaaaagtgag
     7441 cattttttta aattattttt agttaagtga gggcactctt gtctgggggc aaagaaaatt
     7501 cccatgtgcc agcttttgta gtataatagt aagcagacaa aaagatagga atgtgttatg
     7561 aaagtattag cttttgatac gtccagcaag gctctttctc tcgctatttt agaggataag
     7621 caagttcttg ccgagacgac gattaatatt aagaaaaatt acagtattac ccttatgcct
     7681 gccatagatt ttttgatggc aagtttggat tggacaccca aggacttggg ccgaatcgtg
     7741 gtagctgaag ggccgggtag ctatacaggc ttgcgaattg cggtagccac tgccaagact
     7801 ttggctcata ctctgaacat cgagttagtt ggtatgtcta gcctcttggc tctggtaccc
     7861 taccaacaag aaggtttgtt tgtccccttg atggatgcgc gtcgcaacaa tgtttatgca
     7921 ggattttatg aaaatgctaa acctgtcatg ccagaagcgc acctatcttt tgaagaggtg
     7981 ctagaaaaag tcaagggtgc tagtcaggta acctttgtcg gagaagttgc cccctttgtg
     8041 gagcagattc aagaacactt gccaaggact aattataaag aaaccttgcc taatgcagcc
     8101 aatcttgctc ttttagcttg ggacaaggaa gcagactcct tgcatgattt tgtgccgaat
     8161 tacctcaaac gagtcgaggc tgaggaaaac tggctcaaga accataccga gtctggtgag
     8221 tcctacatta aacgcctatg atagaaatca agcgaattca acaacagcct gacctagctc
     8281 aagccatcta cgctgttatg atagctgttt acccagtcag tccttggact ctggagcaaa
     8341 tccaagcaga tctgtcccaa gaccagactt ggtatgcatt ggcttatgat ggggcagaag
     8401 tgattggatt tctagctgtg caggagaatc tttttgaagc agaagtcctg caaatcgctg
     8461 tcaaaggagc ttatcagggt cagggaattg cgtcagcctt gtttgctcaa ttgccgacag
     8521 ataaggagat tttcctcgaa gtcagaaagt caaatcaacg agcgcaagca ttttacaaga
     8581 aagaaaaaat ggcggtcatc gctgagcgaa aggcctacta ccatgaccca gtcgaggacg
     8641 ccattatcat gaagagagaa gtagatgaag gatagatata ttttagcatt tgagacatcc
     8701 tgtgatgaga ccagtgtcgc cgtcttgaaa aacgacgatg agctcttgtc caatgtcatt
     8761 gctagtcaaa ttgagagtca caaacgtttt ggtggcgtag tgcccgaagt agccagtcgt
     8821 caccatgtcg aggtcattac agcctgtatc gaggaggcat tggcagaagc agggattacc
     8881 gaagaggacg tgacagctgt tgcggttacc tacggaccag gcttggtcgg agccttgcta
     8941 gttggtttgt cagctgccaa ggcctttgct tgggctcacg gacttccact gattcctgtt
     9001 aatcacatgg ctgggcacct catggcagct cagagtgtgg agcctttgga gtttcccttg
     9061 ctagccctct tggtcagcgg cggacacaca gagttggttt atgtttcgga ggcaggagat
     9121 tataagattg ttggggaaac ccgtgatgat gcggttggtg aggcttatga taaggtcggc
     9181 cgtgtcatgg gcttgaccta tcctgcaggt cgtgagattg acgagctggc tcatcagggg
     9241 caggatattt atgatttccc ccgtgccatg attaaggaag ataatctgga gttctccttc
     9301 tcaggtttga aatctgcctt tatcaatctt tatcacaatg ccgagcaaaa gggagaaagc
     9361 ctgtctacag aagatttgtg tgcttccttc caagcagcag ttatggacat tctcatggca
     9421 aaaaccaaga aggctttgga gaaatatcct gttaaaaccc tggttgtggc aggtggtgtg
     9481 gcagccaata aaggtctcag agaacgccta gcagccgaaa tcacagatgt caaggttatc
     9541 atcccccctc tgcgactctg cggagacaat gcaggtatga ttgcctatgc cagcgtcagc
     9601 gagtggaaca aagaaaactt cgcaggctgg gacctcaatg ccaaaccaag tcttgccttt
     9661 gataccatgg aataaagagt ggctctttgt caagtgtagt gggtaggcga aaagctacaa
     9721 tctggagagt acgaaattcg tactctcttt tttgatgttc aaagtcatca aaatccgttt
     9781 tttaaaattg ttaaagttcc ggaaaccaaa ggctttgcgc ttgataacct taatcaaatt
     9841 attggtcgct tcaagtttag cgttggaata aggcagctta agtgcgttga taatcttatc
     9901 tctatccctt aaaaaagtcc aaaagaccgt ttgaaaaagc ggatgaaccg ttggcagttc
     9961 ttgctcaatc aatccaaaga aatggtcggc attcttctct tgaaaatgaa agagcaggag
    10021 ttgatagagt tcgtagtgaa cctgtaatcc ctcagagtaa gaaagcagct tatctcgtac
    10081 ttcccgatgg gttaagtgca ttcgaaacgt ggactgatag aatcgtttct gacagagctt
    10141 gaagctatcc tggtgtagta aatcagtcca gtggactgat ttagcccgag cctagaaacg
    10201 aaaaagcgag ggttccaaaa gcgcttgagc gcccgatact ccaaggattt tcggtcaaat
    10261 tggttcatga tttggattcg tactcggttc atagctcggc tcagatgttg gacaatgtgg
    10321 aagcggtcaa gaacaatctt cgcctttgga aagagttgct tagcgagccg ataataaggg
    10381 ctgtacatgt ccatggtgat gacctcgacc agctcccgaa cctctctctg atagcgttgg
    10441 aaatggtttc gaatcaccgc atgagttcgc ccgtctaaaa ttgcgaggat ggatttggac
    10501 tcaaaatctt gggcaatgaa gctcattttg ctctttttga agctatactc atcccaactc
    10561 ataacttttg gcaacttggt ccaatcggtt tcaaacttaa attccctcag ttttcggatg
    10621 acggtggaag ttgagaccgc caatctgtgg gcaatctcag tcatagactg cttttcaagg
    10681 aggagctgag cgattttttg tctcaccatg ttggaaatct ggcaattttt cttgacaatg
    10741 gatgtttgag aaacgaccac cttaaggcaa ttcttgcact gaaagcggcg ctttttgaga
    10801 tgcagtaccg tgggtaaacc ctgacagtcg agaagcggaa ttttagaggc tttctggaag
    10861 tcgtatttga tcatcttccc ttgacaatga ggacaaggag gagcggggga atccaacttt
    10921 gcctgaacga ctagatgggt ttggtatttc agaacagaca agattttgat atttttgtct
    10981 ttcaatccga gcaaatctgt ggtattctta agttgttcca tatgaaccct ttctaatgag
    11041 ttggttagac gcttttcatt ataggtcata tggggctttt tttctacaag aaacgaccct
    11101 ataattcctg gggtgggatt acccactaca gaaattatag agccaaaaca ttccaaagtc
    11161 ttgtctgact ctggaatgtt ttctgttcac agttgttttg taactgccta ataagtaacc
    11221 gcgctataac gaggtatcta ccgagattgt caattctcca gtatactgtt ataaaaaaca
    11281 aaactggagg atttttttat gtccccccat cgtcccatcg actgttccgt agctgcccaa
    11341 taacggagta tagtcggctg aaacaagaat aggacaaaag tgcctcaaga aaagcatcgt
    11401 aatttgacaa tattattctg agatgttttt gatgtaagcc catattttct caatgggatt
    11461 atactcaggt gaataggtag gaagtggtaa cagtctatgc cctgctcctt acacatgttc
    11521 attctgtgaa accttgcatt gtccataatg ataagggatg gtgtatctaa agtaggtagt
    11581 aagaattttt ggaagcaagc ttcgaaaaag tcactcgtca tcgtatcttt ataggtcatc
    11641 ggagccatag tatattgaaa caaaaagggc attagtactt ctataacagt atcgcaatct
    11701 ctggtgtgcg ttgtctcgat cttaattcaa ataattatat cttcttccag agagactaac
    11761 tgcctgctca aggctcgatc atattcgaga taaaaatagg tctcaacccc tgtctcataa
    11821 atataaatag gagtcaggta gcttaagtga ttcaattctt taaggaaccg atttactttt
    11881 tctgggtctt gttcgcagta ggtacggctc ttttttagac tatatcccat agcctttaga
    11941 gcgtaatgaa tagttgttgg aggacagttg aattcagcag ctatttcagt caaataagca
    12001 tcgggatgag tttcaatata gatctttaat ttatctctat caactttttc aattaatagt
    12061 ggaagttttc ttgaagttag tgtattgaaa cgtttttata cagtgctaaa atttgaagtg
    12121 tagtgcagat gtatttgtaa tagtattata gcaattcttt ggagaaggtc gagacaagag
    12181 aaaataggta tctttgagat ataagcatga tttactataa aattattgag ttattcataa
    12241 aaatttctct atcaaatatc ataatgcgtc gtttaaggag atggttacaa tgcaatatag
    12301 ttgtggaaaa ataaatatta atatccctga tggatatgga gacattaagg atatagtgtt
    12361 ttcggctcat attatagtta ggtataataa tggacattgt ggaggtatag atccgcatat
    12421 aattggactt tgtaaaaagc agatcagaag gatgtctcta tatcctattt tgataatcgt
    12481 atctagagat tcaaaggtta ttgatgatta taaaaattta gatattgcct atgttgattg
    12541 tactcaatgt tcaaataatt ttgaaactgc cttacacgtt aaaaatattt taaaattatt
    12601 aaaaatccaa cttatacact gtcatggcta ctcaactaat tattttttat atatgttaaa
    12661 aaaacttgac aagaatggat ttggaaaggt taagacagtg ataacatgcc atggatgggt
    12721 tgaatataac ttaaaaaaga aattcttgac ttattttgat ttttggacat attctatggg
    12781 agatgctttt atttgtgtat ctgaaactat gaaaaaaaga ttggagagta taataaaaaa
    12841 taagaagata gttgcaataa ataacggaat taatgtatca aatagcgatt tagatgtagt
    12901 aggtgttcaa gactttaaaa aagaattttg tattccaaat aacaaaaaaa tcatttgtta
    12961 tgttggaagg ttggatccag aaaaaaggcc ggatagattc cttgaatttg cagaaaaatt
    13021 atttttagtc agagaagatg tgatttttat tatggctgga aatggtagta tgtgggctgc
    13081 tttgaaagaa aaaatttgcc atttgaaatg tagagataat ttcaggttac ttggtgaaat
    13141 ctatccagct actatagttt accaaatatc ggatttattg tatattcctt ctgatacaga
    13201 aggaatacct atgtgtgttt tagagtcaat gtcacaggga actccggttt tagctagtaa
    13261 tgttggcggg ttaagtgaaa ttattgaaca tagggttgat ggatttttat ttgagaagga
    13321 agatgttgag ggagtgtgtg cttgtgctaa ttttttactc aatgattctg agtatttgaa
    13381 atatataggt gagaatagta aatcaaaaat aagaaaacat gagagtatat gatgaattat
    13441 tagagaagag tagtcatgga taggaatata gatcaagaat tagtaagtat tataatacca
    13501 acacataata ggtatgaatc tctgattaga gctgtgaaaa gttgtttaca tcaaagttat
    13561 aaaaatatcg aagttataat tattgatgat aattatagta atgtaaattt aagaaataaa
    13621 attatccatc aatttggcta taccaatcat agaattaagt taattttaag taatgaagat
    13681 ttaggtgcaa ctaatgccag aaacataggt agcaaaaatt ctagaggtaa gtatatatca
    13741 tttttagacg atgatgatga atatatgtca gatcgaattt taaagttgat ggcttgtttt
    13801 aaaaagagta gaatgaagaa tttagcttta gtttatagtt atggaataat aatttatcca
    13861 aatggtacac gagaagagga gaagaccgat tttgttggaa attccttgtt tgttcaaatg
    13921 gttcacaata tagcaggtac gtcattttgg ttgtgtaaaa aagaggtgct agaattaatt
    13981 aatggttttg agaaaataga ttcacatcag gacggtgttg ttttattaaa actacttgct
    14041 caaggatgcc aaattgatat agtgcgagaa ttcttggtga attactacgc tcacagtaaa
    14101 gaaaacggta tcactggagt gacacaaaaa acaattaatg cagatgaaga atattataat
    14161 tactgtagga aatattttaa tttattgagt ttcaatgaga gaatattggt tacaaagaaa
    14221 tattattctt taaacataaa gcggttacta ttaataggag acaaatgcaa ggctttaaaa
    14281 gtaatcaaga aggcaagaga agaaaaaatt tttaacgaat ttcttttttg aaatatatgt
    14341 tattatatca ttttggtttt ttctattgta tatatgacaa ctatgttcaa ttaaaattta
    14401 gaaagtgaga aactattgtg tatactatta taaaatcaaa tataaaaaaa tttagtttat
    14461 taacgatatt tattgttgct ggtcaattat tgctaattta tgcagcaact attaatgctc
    14521 tggtgttgaa tgaattaatt gcgatgaatt tagagcggtt tttgaaattg tcaatctacc
    14581 aaatgattgt ctggtgtggg ataatattcc ttgactgggt agtgaaaaat tatcaggttg
    14641 aagtgatcca agagtttaat ctagagattc gaaatagagt tgccacagac atctctaact
    14701 ctacctatca agaatttcat agtaaatcat caggaacata tctttcgtgg ctaaataatg
    14761 atgttcagac tttaaatgat caggctttta aacaactttt tttagtaata aaaggaattt
    14821 ctggtactat atttgcagtt gtgactctta atcactatca ttggtcattg actgtagcca
    14881 ccttgttttc attaatgatt atgctacttg taccaaaaat ctttgcatcg aaaatgcgag
    14941 aagttagtct aaatttaact aaccaaaatg aagctttttt aaaatctagt gagactatat
    15001 tgaatggatt tgatgtgtta gcgtccttga atcttttata tgtattgcct aagaaaatta
    15061 aagaagcagg aattttatta aagatggtta tacaaagaaa gacaactgta gaaacgttag
    15121 caggcgctat tagcttcttt ctcaatattt tttttcagat atctctcgtt tttttaacag
    15181 gctatcttgc aataaaagga atagtgaaaa ttggtactat tgaagcaata ggagcactaa
    15241 caggtgttat ttttacagcg ctaggtgaat taggaggtca attatcctct attattggta
    15301 cgaagcctat ttttttaaaa ttgtattcaa ttaatccaat tgagtcaaat aaaatgaatg
    15361 atatcgaacc aaatgaggtg aatagagatt ttccgttata tgaagcaaaa aatatttgct
    15421 ataagtatgg agataaagaa atattaaaaa acttaaattt ttgttttcaa cgtaatgaaa
    15481 agtatttaat tttaggtgaa agtggaagcg ggaaatctac attattaaaa ttattgaatg
    15541 gctttttgag agattatagt ggagaattgc gattctgcgg ggatgatata aaaaaaacct
    15601 cctatttaaa tatggtttcg aatgttctat atgtagatca aaaagcttat ttgtttgaag
    15661 gtacgattag agataatatt ttattggaag aaaattatac tgatgaagaa atactacagt
    15721 ctttagagca agttggtttg agtgtaaaag attttcctaa taacatttta gattattatg
    15781 ttggtgatga tgggagatta ctgtcaggag ggcagaaaca aaaaattact ttagctagag
    15841 ggctaattag aaataagaaa atagtattaa ttgacgaggg aacttctgct atcgatagga
    15901 gaacttcgtt agcgattgaa cgtaagatat tagatagaga ggatttgact gtcattattg
    15961 ttacccatgc tccgcatccg gaacttaaac aatattttac taagatatat caatttccaa
    16021 aggattttat ttaagaatat aggggaggga aacatgagat tctattgtga agcttatgag
    16081 gtaattactg aagagaaaaa agtatcattt ttttatgtag atgagaaacc cttaaggtat
    16141 ctgttatttg actatatatt tctactagtt ctagctaaaa aaaaatatcc caatcttatg
    16201 aact
//
LOCUS       DAVKJU010000048        15886 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVKJU010000048 DAVKJU010000000
VERSION     DAVKJU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.048"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            46..288
                     /locus_tag="VW191_001472"
     CDS             46..288
                     /locus_tag="VW191_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624220.1"
                     /translation="MKINEYLWRTSCLILLIIMSFFAGFGAKLEFTSYNPFLSVKFGL
                     VVALTVFVVQLLAILPAMVKRGNKNENVESNNSCEE"
     gene            251..1642
                     /locus_tag="VW191_001473"
     CDS             251..1642
                     /locus_tag="VW191_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEU9624221.1"
                     /translation="MRMLNQTIRVKSKYPYLIRPRNIKIVRLFIQIFVFLLLISSWIA
                     IIPFIARLVFSMFFISLLFFVEKFFRSNFIAKLRNKLNIREALFYMLISLNLYDELDN
                     EVVDTAVLYFDVENNKVVVCAPLFGNRYLKTLKNLEEYLCPTLGLSLLSKKEEIDKIV
                     YVLGQKEEIEQYVFNSNTLTREFFKDIPSSIIKLSNTQKFSLKSNTNLGIYGRTGTGK
                     TIALQWYLFNALAKGCGIADNTYLGIVDGKAADLYRIGELLHEELGEQVAVGSSPQML
                     AQLSRKFIENMDARFKIIKQNSSLNADIYELDELGLVPNFLFVDELASIRDSCGSTKQ
                     GRETWNEILQNLGLIARKGRQAGCHLVLSTQDPNAENIPVELRNQISAVLYLGAPGAD
                     RLKMAFSMCELENVPTVSDRKGEALFYADGLNTVEPVLTIVPFVDLKTKKEFLNVVRN
                     ILPTNDGLPPLAK"
     gene            1898..2317
                     /locus_tag="VW191_001474"
     CDS             1898..2317
                     /locus_tag="VW191_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624222.1"
                     /translation="MVYNMTDTNIKIGLLDLEMSQFDIPKKVVILSVRVNNKYKEVNG
                     ENVKTDEVSKITSAVLDADKVKVLTEMGISTDDLKAINLEIIGNLDKIAKLAQNDALL
                     NVPVELIRPKVRLAWNMARNNWSGVKIVCEDIKVLGE"
     gene            2322..3341
                     /locus_tag="VW191_001475"
     CDS             2322..3341
                     /locus_tag="VW191_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEU9624223.1"
                     /translation="MNTVRIDYFAVTVKNISPEKVLTDILLIPLENFTLNDWGINKYK
                     QHYSCSEIKVYFNADRLSMGVFIELKGQGCRQYEEFLNSNENNWIALISRLYHHNANF
                     TRLDIAHDIFDGSLDVQRIYDYCKKGLCISKAKHFEYHEKSVLDSGERVGETVAIGSR
                     GNQQWCIYNKRMEQLGKQELVEVSSWTRAELRCWQEKANIIAEQLYLKRPLSSIYFEA
                     INGHYRFIRPNAIDSNRWRRKKVEWWVNYLGTENQTVLSITRAKTTLRESEVWTEKQV
                     AKTLAKVYIAKYQAYDVQKAEDYIQNLLKEGLSKLTDNDEKDIEQYIREQNSSQLWGT
                     DGRSD"
     gene            3345..3527
                     /locus_tag="VW191_001476"
     CDS             3345..3527
                     /locus_tag="VW191_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEU9624224.1"
                     /translation="MADTISNKDLISMGYRPSTANAIIHQARELLVSRGYTFYNRKRL
                     MVVPKSVVKELLGMEL"
     gene            3529..3942
                     /locus_tag="VW191_001477"
     CDS             3529..3942
                     /locus_tag="VW191_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEU9624225.1"
                     /translation="MAYIEYKQRGKKRLWSFSIRERSKSLLHKSGFKTKREAKIEAEK
                     VLHKLNTGSVLSSSMTLSELYNEWLDLKILPSNRSVVTKKKYLMRKKVIERLFGNKPV
                     SQIKPSEYQKIMNEYGETVSRNFLGRLNSCIRASI"
     gene            3943..4656
                     /locus_tag="VW191_001478"
     CDS             3943..4656
                     /locus_tag="VW191_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9624226.1"
                     /translation="MAIADKVIIEDFTAYVELFSSKSGQKVEEKYLHAESDYQKVLVY
                     LKNKFDYQKSIVPYVIYFLFKTGMRFSELIALTWDEVDELNEQLKTYRRYNTAIHKFT
                     PPKNNTSIRLVPITSDMLSLLKTLKILQLKTNKELNIDNENNLIFQHFGYVYDVPDIA
                     TVNKAIKVMLKELQIFPLITTKGARHTYGSYLWHNNIDLGVIAKILGHKDISMLIDVY
                     GHTLEEKISEEFMAVKSLL"
     gene            complement(4739..4811)
                     /locus_tag="VW191_001479"
     tRNA            complement(4739..4811)
                     /locus_tag="VW191_001479"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4776..4778),aa:Lys,seq:ctt)
     gene            complement(5076..6347)
                     /locus_tag="VW191_001480"
     CDS             complement(5076..6347)
                     /locus_tag="VW191_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU9624227.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            6617..6856
                     /locus_tag="VW191_001481"
     CDS             6617..6856
                     /locus_tag="VW191_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624228.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVYSNNGLKKYEK"
     gene            7021..7200
                     /locus_tag="VW191_001482"
     CDS             7021..7200
                     /locus_tag="VW191_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624229.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            7343..7516
                     /locus_tag="VW191_001483"
     CDS             7343..7516
                     /locus_tag="VW191_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEU9624230.1"
                     /translation="MTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGELN
                     KYTERGIGKQAGL"
     gene            7553..8005
                     /locus_tag="VW191_001484"
     CDS             7553..8005
                     /locus_tag="VW191_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEU9624231.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            8296..8766
                     /locus_tag="VW191_001485"
     CDS             8296..8766
                     /locus_tag="VW191_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEU9624232.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDTLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            8822..9364
                     /locus_tag="VW191_001486"
     CDS             8822..9364
                     /locus_tag="VW191_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624233.1"
                     /translation="MLEKVKQFFRSRSAKTEPSVDILPRNRFADLDFERVLKSGARRL
                     VNEEGRYAEDGKITELEFPEDFAEFEFLVGFKTEEEEQFQQLLARLNSIDNAIQSYLE
                     SEMQQPIPQYAKDLGYTQKRWEKTFYFHPWILSGEENPPNLRYVADYVNDEFTVYFAK
                     KHGRWQAYWDAECQKVIEES"
     gene            9414..9848
                     /locus_tag="VW191_001487"
     CDS             9414..9848
                     /locus_tag="VW191_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9624234.1"
                     /translation="MELLNSRMDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPG
                     GGREGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQF
                     DNIIFGDEGQGYKLMPIEEFLNSKQAVPQLQGRLRDYLEESL"
     gene            9845..10318
                     /locus_tag="VW191_001488"
     CDS             9845..10318
                     /locus_tag="VW191_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624235.1"
                     /translation="MITLVRAGAEDLETIIAIQRASFKAVYEKYHDEYDPYLEDCERI
                     KWKLVERPNSYYYFVKEDEENIGFLRVQTNAELTKAWLGTAAILPQYQGKGYGSKGLS
                     LLEKEFPTITQWDLCTVLQDAGMVAFYEKNGYRQIHIEAEKEGMDMVYMKKLIEK"
     gene            10453..11406
                     /locus_tag="VW191_001489"
     CDS             10453..11406
                     /locus_tag="VW191_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU9624236.1"
                     /translation="MMETWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNV
                     DRFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARISELTDFMDLGELKIGTTAL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKM
                     SIFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNP
                     GMENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDK
                     WDMVRELAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            11408..12151
                     /locus_tag="VW191_001490"
     CDS             11408..12151
                     /locus_tag="VW191_001490"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEU9624237.1"
                     /translation="MQQYFVKGSAISPVTIENKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESGKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            12162..13958
                     /gene="pepF"
                     /locus_tag="VW191_001491"
     CDS             12162..13958
                     /gene="pepF"
                     /locus_tag="VW191_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEU9624238.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLSDMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            14205..14996
                     /locus_tag="VW191_001492"
     CDS             14205..14996
                     /locus_tag="VW191_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEU9624239.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLLDHLGDPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEGVSSDVGLDKEIDKVGLPTLERSFRALVYANLLYADANQ
                     QSVFYQGLQLEIRNVFSNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPINRIHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFSKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            15107..15775
                     /locus_tag="VW191_001493"
     CDS             15107..15775
                     /locus_tag="VW191_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU9624240.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGFDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGPIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
BASE COUNT         4872 a         2717 c         3638 g         4659 t
ORIGIN      
        1 taatctctct ctttattttt aaaatttgaa aggaaaaata caaaaatgaa aattaatgaa
       61 tatttatgga gaacgagctg tcttatactg ctaattatca tgagcttctt tgcaggcttc
      121 ggagctaaat tagagtttac atcttataac ccgtttttat ctgttaaatt tggtttggtc
      181 gtagctctga cagtctttgt agttcaattg ttagccatac tgccagccat ggttaaaaga
      241 ggaaataaaa atgagaatgt tgaatcaaac aattcgtgtg aagagtaaat acccttactt
      301 gattcgtccc cgaaatatta aaattgtaag attgtttatc caaatttttg tctttttact
      361 cttaatatct tcgtggattg ctataatccc atttattgct cgtctagtct tttccatgtt
      421 ttttataagt cttctttttt tcgtagagaa gttctttcgt tctaatttta tagcaaaatt
      481 gagaaacaag ctcaatatca gagaagcctt gttttacatg ttaattagtt taaatttata
      541 tgatgagctc gataatgaag tagtagatac agcagtttta tattttgacg ttgagaataa
      601 taaagttgtt gtctgtgccc cactatttgg gaaccgttat ctgaaaacct tgaaaaattt
      661 agaggaatat ctctgtccca ctttgggctt gtccttgctg tctaaaaaag aagaaatcga
      721 taaaatagta tacgtgttag ggcagaaaga agaaatagaa caatatgttt ttaatagtaa
      781 cacattaaca agagaatttt ttaaagatat tcctagttca ataatcaagc tgtcaaatac
      841 acagaaattt agtctaaagt ccaacactaa cctaggtata tatggaagaa cggggacagg
      901 caaaacaata gctctgcaat ggtacttatt taacgcactg gctaaaggtt gtggtattgc
      961 tgataatact tatttaggca tagtagatgg taaggcagct gacttgtatc gtattggtga
     1021 acttcttcac gaagaactgg gagaacaagt ggcagtaggc tcttcaccac aaatgcttgc
     1081 ccaattatcg agaaaattca ttgagaatat ggatgcaaga tttaaaatta tcaaacagaa
     1141 tagttcattg aatgctgaca tttatgagct agatgagctg ggactggtac ctaatttttt
     1201 gttcgttgat gaactcgctt ctattcgtga tagttgtggc tctacgaaac aaggtagaga
     1261 aacatggaat gaaattctac aaaatttagg cttgattgca cgcaaaggac ggcaggcagg
     1321 ttgccaccta gtcctatcta ctcaagaccc taatgccgaa aacatccccg ttgaattgag
     1381 gaatcaaatt tcagcagttt tataccttgg tgccccaggg gctgatagat tgaagatggc
     1441 ttttagcatg tgcgaattag aaaacgttcc tacagtttca gatagaaaag gggaggctct
     1501 cttttatgct gatgggctta atacagtaga gccagtgcta acaattgtgc catttgttga
     1561 tttaaaaact aagaaagagt ttttaaatgt agttagaaac atactgccaa ctaatgacgg
     1621 tcttcctccc ctagcgaaat agggcgattg ccggaggcag aatcgagcgg agcgctctct
     1681 tttcgctagg ggagggagag ccaaccgact gctttgcagg aggtcagaaa aaacatcccc
     1741 agtactaata gggggtgcac acatacatga aatgtactct aaacccttga tacgtgaggg
     1801 gttacggctt actagttgca ccaaggggtc gcaaactctt gggagagtag ggatgagagg
     1861 tgtttccatg tatgatacgg aattaaagga gaaaaatatg gtttacaata tgacagatac
     1921 aaacattaaa attggattgc ttgaccttga gatgtcacag tttgatattc ctaagaaagt
     1981 agtgatttta tcagttaggg tgaacaataa atacaaagaa gtcaacggcg aaaacgtgaa
     2041 gacagatgaa gtaagtaaaa ttacttctgc tgttcttgac gctgacaaag tgaaagttct
     2101 aactgaaatg ggaatttcta ctgatgactt aaaagctatt aacctagaaa ttatcggaaa
     2161 tctagataag attgcaaaac ttgctcaaaa tgatgctttg ttgaatgttc ctgttgaatt
     2221 gattcggcct aaagttcgac tagcttggaa catggctagg aataattggt ctggtgtaaa
     2281 aattgtgtgt gaggatatta aagttctagg agaataatcc tatgaataca gttagaatag
     2341 attattttgc tgtgacagtt aagaatattt cgccagaaaa agttctgaca gacatccttt
     2401 tgattcctct tgaaaacttt accttgaatg attggggcat aaataagtat aagcaacatt
     2461 attcttgttc agaaattaag gtgtatttta atgctgatag gctttctatg ggtgtattta
     2521 ttgaattaaa agggcaaggt tgtcgtcaat atgaagaatt tttaaacagt aacgaaaata
     2581 attggattgc tttgataagt cgtttatatc atcataatgc caactttact agactagata
     2641 ttgcccacga catatttgac ggtagcttag atgttcaacg aatatatgac tattgcaaga
     2701 aaggattatg tatctcgaaa gcaaaacact ttgaatatca tgagaaatca gttctagatt
     2761 ctggtgagcg tgtaggagaa acagttgcaa taggttcacg aggtaatcag cagtggtgca
     2821 tatataataa acgcatggag caacttggta agcaagagct tgtagaagtt tcatcttgga
     2881 ctcgcgcaga gcttagatgt tggcaggaga aggctaatat aattgcagag cagttatatt
     2941 taaaacgtcc cttatcgtca atatattttg aggctatcaa cggtcattat cgctttattc
     3001 gtccaaatgc tatagatagt aatcgttgga gaagaaaaaa ggttgagtgg tgggtaaact
     3061 atctagggac agaaaatcag acagttctga gtattacaag ggctaagacg acgttacggg
     3121 agtctgaagt ttggactgag aaacaggttg caaaaacatt agcaaaagtc tatattgcaa
     3181 agtatcaggc ctatgatgtt caaaaggcgg aagattatat tcaaaacttg ctcaaagagg
     3241 gactttcaaa gctaactgat aatgatgaaa aggatataga acaatatata agagaacaga
     3301 atagttccca actttgggga accgatggaa ggagtgactg acatatggca gatactatat
     3361 caaataagga tttgatttcc atgggctata ggccatcaac agctaatgct attatccatc
     3421 aggcgagaga attacttgta tcacgaggct atacatttta taatcgcaaa cgtttgatgg
     3481 ttgttccaaa aagtgttgtg aaagagttgt tgggaatgga attgtgaaat ggcttatatc
     3541 gagtataaac agcgtggtaa gaaaagactt tggtccttct ctatacgaga aaggagcaag
     3601 agcctactcc ataaaagcgg atttaaaaca aaacgagaag ctaaaataga agcggagaaa
     3661 gttcttcata agttaaatac tgggagtgtc ttaagctcta gtatgacttt atcggagctt
     3721 tataatgaat ggcttgattt aaaaatttta cctagcaata gaagtgtagt tactaaaaaa
     3781 aaatatctca tgagaaagaa ggtcatcgaa aggttatttg gaaataagcc tgtatcacaa
     3841 attaagccta gcgagtatca aaaaattatg aatgagtatg gagagactgt atcgagaaat
     3901 ttcttaggaa gattgaattc ttgtattcgg gcaagtatat agatggctat tgctgataag
     3961 gtgataatag aggattttac tgcttatgtt gagttgttct cttctaagag tggacaaaag
     4021 gttgaggaaa agtatttgca tgctgagtca gattatcaaa aagttttagt atatttgaaa
     4081 aataagtttg attatcaaaa atctattgta ccgtatgtaa tttatttcct ttttaaaact
     4141 ggcatgcgtt tctccgaatt gattgctcta acttgggatg aagttgacga gcttaatgag
     4201 caacttaaaa cttatcgtcg ttacaacacc gctattcata agtttactcc acctaaaaac
     4261 aatacttcaa taagattagt tcctattact tcagatatgc tttccttgtt aaaaacattg
     4321 aaaatattgc aattaaaaac aaataaagaa ttaaatattg ataatgaaaa taatttgatt
     4381 tttcagcatt ttggctatgt ctacgatgtt ccagatattg caacagtcaa taaagctata
     4441 aaagtaatgt tgaaggaatt gcaaattttt ccattgatta ctaccaaagg agctagacac
     4501 acttatggta gttatttatg gcataataat attgatttgg gagttatagc gaaaatttta
     4561 ggacataaag atatctccat gttgattgac gtttatgggc ataccttgga ggaaaaaatt
     4621 agtgaggagt ttatggctgt gaaaagtttg ttgtaaaaac acggcaaatt gcacgacaat
     4681 ttttaaaagg aaagcaaaaa aagccctata ttataaggct tttcctgttg tatttagatg
     4741 ccccctacag ggatcgaacc tgtgacccac ggattaagag tccgctgctc tgccagctga
     4801 gctaaggagg caaagaaaaa agctgtattg gtgccgaaac ttcacggttt gtattgaacc
     4861 cgcgcaatta agcaggtggg caactcgctc taactgaagc tgtttccgtg tgagacggcc
     4921 tacatgctgt tagaagactt ttgtttccct aataatacaa aaaatagtcg gtcaacactt
     4981 aagtgtgaag tcgtacacca cagcgtttct atatttatat gataccactt tttcaaaaaa
     5041 aatcaagagg aaagtgcaat tttttgaaaa ggatttcaga tttttcgcaa acggtcgata
     5101 gcttcctttc tttgagccaa agcccgttca tatttaccag tatcttctgc ttggaaatag
     5161 tgatgattac gaattttttc tggcagatag tcttgcttga cccaatttcc aggatagttg
     5221 tgtggataga gatagtcttg ggcattcccc agttccttgc ttccactgta gtgcccatca
     5281 cgcaggtgtc gcggaatagg caagtgccct gatgttttga ggtcagcaag tgccttatcc
     5341 atagctacat aggctgagtt ggattttgga gaaagggcca aatcaatcac gacattggca
     5401 atgagaatgc gggcttcggg gaaaccaatc ctttgtgcag catccagagc agtcacggta
     5461 tgaatctggg cctcaggatt ggctaagccg atatcttcat aggcgataac agtcaagcga
     5521 cgagcgagac taggcagatc cccagcctca atcaagcggg cagtatagtg gagactggca
     5581 tccacatctg agccacgaat agacttttgc agggcagata gaacatcata gtgaccgtct
     5641 ccatccttgt ccatagtgat gtagcttctc tgaagactat tttccatgat gtctaaggtg
     5701 atgtggcgaa tgccctcgtc attctcaggg gtagagagaa cagccaagtc cagtgagtta
     5761 aaggcagagc gaaggtctcc gtttgtagag gttgcgatga aatccagcgc atcctcatct
     5821 agttctattg gaaaatcaaa accacgttca gggttactta gagctatctg cagggcctct
     5881 ttgacgtctt ggttagacag aggttccaac tcgaaaattt gaacacggct acgaatggca
     5941 ggagtgacag agaagaaagg attttcagtc gttgctccaa tcatgatgac cagtccactt
     6001 tccaagagag gcaagaggaa gtcttgcttg gtcttatcta gtcgatgaat ttcgtctagc
     6061 aataggacga gaccaccaga aaatttagct tcttccgaga tttcttgcag tcgcttttta
     6121 ctatcaactg tcgcattgaa agttcgaaag gcatacttgg tcgttccagc gatggcagag
     6181 gcaatactgg ttttgccgat tcccggaggg ccatatagaa tcatggagga caggcggttg
     6241 gcttccacca tgcggcggat gatttttcca ggtccgacca gatgctcctg accgatgacc
     6301 tggtcgatgg ttttagggcg catgcgaagc gcgagattgt ctggcatagc agtcctttct
     6361 aacgtggatt tgagtagaat gactcatcta ctatatctta ctatgtccat tctagcctat
     6421 tagttgtgat atttcaatgg ttttgtaata atcagggagt gagatagaat cttttttact
     6481 atttaattta atgaggagag gtatcagcat gagagatttg attttcctta aagctcaaaa
     6541 taggatagtt gagcctaaag aagtggaagt gctgacttga gaaataatat tgctgaatgt
     6601 cattgtatga actataatga aaagagaggt gataagcaat ggaaatgatg gaccaagtca
     6661 ggaaattttg atttttacaa aacaaattcg tcattggatt cttagtgatc aagttatttc
     6721 agggaaaaga aaacttttct tccgggaaga tactccaaaa gagattttag atttgtacga
     6781 aaacattaaa tctaaacttg attttgctta ccaagaagtt tattctaata atgggttgaa
     6841 aaaatatgaa aaatagaata attgatgttt ttgaagtagt aaatcgcatc cttgtaataa
     6901 ctgttgaaaa tcctaatttc gaagacttga gagttaatca tactcaatga aaatcaaaga
     6961 gcaaactagg aaactagccg caggctgtac ttgagtacgg taaggcgacg ttgacgtggt
     7021 ttgaatttga ttttcgaaga gtatcagttt gtaaaaattg gagataaaaa atatagagta
     7081 cgcagtggtc ctatgattca ttcaactccg cctcaatctg tcttagatcg agatactttt
     7141 acaattgact atacagatga tgaattgctc gataaagaag cagtttttac tactcattag
     7201 aaaaataggt ctgaataaat tggtttccct tagaaccaca tctaggggct tttatgtata
     7261 atagaataat aagtatcact cctttacaat acgtgtaaca cgtgttataa tggtgttgtc
     7321 aggaggtaaa agcgctatac ctatgacaca aaaagagatg gttaaactcc ttacggccca
     7381 tggttggata aagactagag gcggtaaagg ctcccatatt aaaatggaga agcaagggga
     7441 aagacctatc acaatccttc atggtgagct gaataagtac actgaaagag ggataggaaa
     7501 gcaagctggg ttgtaaaatt cagtccctgc tttttgatat ggaggtagtg aaatgttagt
     7561 tacgtatcca gctttatttt attatgacga cacagacgga acagaagcga cttattttgt
     7621 ccatttccca gattttgaat actcagctac acaaggagag gggatttctg aggctttggc
     7681 tatggggtcg gagtggctag ggataactgt tgcagatttg atagagagtg atggagagtt
     7741 acctcagcca tcagatatca atagcttgtc tttaattgat aatgatcctt ttaaagatga
     7801 tgaagatttc gtgtcaacct atgaccttga taaatctttt atttctatgg tatctgttga
     7861 tgtatcagaa tacctaggaa gtcaggaacc cattaaaaag actttgacca taccaaaatg
     7921 ggcagataag ttgggacgag aaatgggact taacttttct cagactttga cagacgctat
     7981 tgcagataag aaagttcaag cctaagatta agcgtggtat cgtggtaata gtcaataaaa
     8041 aagcacgtcc acttgtgcta gttactcgcc tattgatttt atagatttat tagccctttc
     8101 gcgggctttt tatattgatt tttatgagaa atgaagaaat cagactttta agaaaatcac
     8161 tgataccaag ggtttaaatg aggtaatatg gtataattag gacataaaat aattttgtgg
     8221 taagatggta gtatctattt tagcatattt ccgagcaatc ggggcgatta aagagtcgca
     8281 tagaaagagg acaaaatggc aacatacgga tttttagatg ttttagagga agagttggac
     8341 aagaactttc cctttgactt tgagattagc tgggacaaac gcaaccatgc ggtagaagtg
     8401 agttttctat tggaagcaca aaatgctgca ggtgtggaga tggtggatga agacggagag
     8461 gtttcatcag atgacattct ttttgaggaa gcggtccttt tctacaatcc tgctaagtca
     8521 accgtcaatg cagaagacta tttaacggtt atcccttatc tacctaaaaa aggattttct
     8581 cgcgagtttt tagcttattt tgcccttttc ctcaaagata ctgcggaagt tgggctagat
     8641 accctcatgg actttttgga agatccagaa gcagaggaat ttgttatgga atggaatcaa
     8701 gaagtctttg aagaaggaaa agttggcttg gaagagggag aattttaccc ttatccgagg
     8761 tattagaaaa gattctaagt ggacattttt aaataatgaa ttcaaggaga agggggagat
     8821 tatgctagaa aaagtcaaac agtttttcag gagcaggtca gctaaaactg agccgtcagt
     8881 tgatattctt ccccgcaatc gctttgcgga tctagatttc gagagagtat tgaagtcagg
     8941 agctcgtcgc cttgttaatg aggaaggacg ctatgctgag gatgggaaaa tcacagaact
     9001 tgaatttcct gaagattttg cggaatttga atttctagtt ggttttaaga cggaggaaga
     9061 agagcagttt cagcaactat tagctcgttt aaatagtatt gacaatgcta ttcagtccta
     9121 tttggaaagt gagatgcaac aacccatccc tcagtatgcc aaggatctcg gctatactca
     9181 gaagaggtgg gagaaaacct tttacttcca tccttggata ttaagtggtg aggaaaaccc
     9241 gcctaatctt cgctatgttg cagattatgt aaatgatgag tttacggttt attttgctaa
     9301 aaaacatggc agatggcaag catactggga tgcagagtgc caaaaagtaa tcgaagaaag
     9361 ctagctcgta ttttcctaga gagtaattgt tatcatagaa aagagtaaat atcatggaat
     9421 tattgaatag cagaatggat ttccaaggtt gcaagattgc cctcttttgt ggggataagc
     9481 tactgacaat tttgcgtgat gacaaggacg atatcccttg tcccaacatg tgggagttgc
     9541 caggtggtgg ccgtgaaggg gacgaaagcc cttttgaatg cgctcggcgt gaagtttatg
     9601 aggaactggg aattcattta gatgaagact gtctgctttg gagcaagatt tatcctagcg
     9661 taatttttaa aggcaagaaa tcggtcttta tggttggtca gctaagacaa gaacaatttg
     9721 acaatatcat cttcggagat gaaggacagg gctataaatt gatgcctatt gaggaatttc
     9781 tcaattctaa acaggcagtg cctcagttgc agggaagatt gagagattat ttggaggaaa
     9841 gtttatgata acacttgttc gagcgggagc agaggattta gaaactatta ttgcaattca
     9901 gagagccagc tttaaggctg tttatgaaaa ataccatgat gaatatgacc cttatctcga
     9961 ggactgcgaa cgaattaagt ggaaattagt cgagcgtccc aatagctatt actactttgt
    10021 aaaagaagat gaagaaaaca tcggattttt acgagttcag accaatgccg agttaacgaa
    10081 agcttggttg ggaacggcag cgattttgcc tcagtatcaa ggaaaagggt atggatctaa
    10141 aggtttaagc ttactggaaa aagaatttcc aaccatcaca caatgggatt tgtgtacggt
    10201 gttacaggat gcaggcatgg ttgcttttta cgagaaaaac ggctatcgtc aaatccacat
    10261 tgaggctgaa aaagaaggca tggatatggt ctacatgaaa aaattgatag agaaatagaa
    10321 aggatggttc agttaaattt ctaaactgaa cccgccctaa acactgtgcc aaaaagataa
    10381 acttctctta gacacaagcg tcttcagaga atttcctatt ttggttttgt gttttacggg
    10441 cttggtatct taatgatgga aacatggcaa gagttaaaag ttacagtgaa gcgtgaggga
    10501 gaggagttgg tttccaatct cttgattgag ctgggagcgc aaggtgttgc gattgaagac
    10561 agtatggact atgtgggaaa tgttgaccgc ttcggcgaga ttttcccaga ggtcgagcag
    10621 caagaagaaa tcgtagtgac agcctactac cctgacacgg tggatgtgac agtggttgag
    10681 gcggacttgc aggctcgcat atcagaattg acggatttta tggatttggg agagttaaaa
    10741 ataggtacga ctgccttggc tgaggaagac tgggcagata actggaagaa atactatgag
    10801 ccagctcgta tcactcatga tttgaccatc gttccctctt ggacagacta tgaggcgact
    10861 gcgggagaaa aaattatcaa gctggatcct ggcatggctt ttggtacggg aacccaccca
    10921 actaccaaga tgagcatttt tgccttggaa caggttcttc gtggtggcga aacggtgcta
    10981 gatgtgggaa ctggttcagg ggttctctct attgccagct cgcttctggg ggctaaagaa
    11041 attttcgcct atgacctgga tgatgtggca gttcgtgtgg ctcaggaaaa tattgagctc
    11101 aaccctggaa tggaaaatat ccatgtagcg gcaggcgatt tgcttaaagg cgtggagatt
    11161 gaagcagatg tcatcgtggc aaacatcttg gcggatatcc tcattcatct gacagaggat
    11221 gcttatcgct tggttaagga tgaaggttac ctgatcatga gtggcattat caaggataag
    11281 tgggacatgg tgcgcgagtt ggctgaatca gctggatttt tcctcgaaac tcacatggtt
    11341 caaggggaat ggaatgcctg tgtctttaag aaaaccaagg atatctctgg tgtgattgga
    11401 ggctagcatg cagcagtatt ttgtaaaagg cagtgctatc tctcctgtca ctatcgagaa
    11461 caaggaaacc agtaagcata tgtttcaggt tatgcgcttg aaagaagagg atgaggttac
    11521 cttagtcttt gatgatggca tcaagcgctt ggcgcgcgtg ctggatatgg aaaatcgtca
    11581 gtttgagttg gtccaagaat taaatgacaa tgtggaacta ccagttcaag tgaccatcgc
    11641 atctggattt cccaagggag acaagctgga gttcatcgct caaaaagtaa ccgaactggg
    11701 tgctagccaa atctgggcct ttcctgcaga ctggtcagtt gccaagtggg atggcaagaa
    11761 attgggtaaa aaagttgaaa aactagaaaa aattgccctt ggagcagccg agcaaagcaa
    11821 gcgtaatatt gtaccaagta ttaaactttt cgagaaaaaa gcagattttc taactcagct
    11881 ggaccagttt gactctatca tagtagccta tgaagaatca ggtaaagaag gagaagccgc
    11941 tgcgcttctg caagcagtca ctggtcttga aacaggagcc aaactgctct ttatctttgg
    12001 tccagaaggc ggtctctcac ctgcagaaat cgaaagtttt gaagctaagg gagccgtttt
    12061 ggtaggccta ggtcctcgta ttttgcgagc agaaacagca ccgctttacg ccttatcagc
    12121 ccttagtgtt ttagtagaat tagagaaata agaggaagaa aatggaacaa aaacaccgtt
    12181 cagaatttcc agagaaggaa ctctgggact taacagccct ataccaagac cgtgaggatt
    12241 tcttgcgtgc aatcgagaaa gctcgtgaag acatcaacca atttagccgt gactacaagg
    12301 gtaatcttca tacttttgag gatttcgaga aggcctttgc ggaattggaa cagatctaca
    12361 ttcagatgag ccatattggc aactatgctt ttatgcctca gacgacggac tatagcaatg
    12421 acgaatttgc caatattgcc caagctggga tggaatttga aacagatgcc agcgtagcct
    12481 tgaccttctt tgacgatgcc ttggtggtag cagacgagga agtcttggac cgtttgggtg
    12541 aattgcctca cttgacggca gctattcgtc aggccaaaat caaaaaagcc cactatctag
    12601 gggctgatgt ggagaaggcc ttgaccaatc tcggtgaagt tttctatagt ccgcaggaca
    12661 tttatactaa gatgcgagct ggggattttg aaatggctga ctttgaagcc catggcaaga
    12721 cctataaaaa cagctttgtc acctatgaga atttctacca aaatcacgag gatgctgagg
    12781 ttcgtgagaa atccttccgt tccttctcag agggacttcg taagcaccaa aatacggctg
    12841 cagcagccta tctggctcag gtcaagtctg aaaaactctt gtctgatatg aagggatacg
    12901 actctgtctt tgactatctt ctagctgaac aagaagtgga ccgtgtcatg tttgaccgcc
    12961 agattgacct catcatgaag gactttgcac cagtcgctca gagatacctc aagcatgttg
    13021 ccaaggtaaa tggtcttgaa aagatgacct ttgcagactg gaaattggac ttggacagcg
    13081 ccctgaatcc tgaagtgact attgacgatg cctatgattt ggtcatgaag tcggtagaac
    13141 ctttggggca agaatattgt caggaagttg ctcgttacca agaagagcgc tgggtggact
    13201 ttgctgctaa cagtggcaag gattccggtg gttatgcggc ggacccatat cgcgtacacc
    13261 cttatgtcct catgagctgg acaggccgtt tgagcgatgt ctataccttg attcatgaaa
    13321 tcgggcattc tggtcaattc atcttttcag acaatcatca aagttacttc aatgcccata
    13381 tgtcgaccta ctatgttgaa gcaccgtcaa ccttcaatga attgctactc agtgattact
    13441 tagagaacca atctaatgac ccacgtcaaa aacgctttgc tctggctcat cgcttgacag
    13501 acacctactt ccataacttt atcacccacc tcttggaagc cgccttccag cgtaaggtgt
    13561 atacattgat tgaagaagga gaaaccttcg gagcaagcaa actcaacagt attatgaagg
    13621 aagttttgac agatttttgg ggcgatgcca ttgaaatcga cgacgatgca gctttgactt
    13681 ggatgcgcca agctcactac tatatgggct tgtatagtta cacttactct gctggtcttg
    13741 tcatctcgac agctggttac cttcatctta aaaactcaga aactggagct gaagactggc
    13801 tcaatctcct caaatcaggt ggtagcaaga caccacttga gtcagccatg attatcggtg
    13861 ctgatatttc aacagacaaa ccactccgtg ataccatcca attcttgtct gacacagttg
    13921 accagattat cgcctacagt gctgagttgg gggagtaagg ggaatagaaa aattttttag
    13981 aatgatgttt atgggcttgt taagcgtcat tctatttgtc ttagttgtag ctattttcaa
    14041 aaatgtcgtt gaaggtcgtg atgttaaatt tgaacttact gtagaactgg tattgatagt
    14101 tatttacgtc attttatatc agattgtatt taattgagaa ttcaatattg agtcatttaa
    14161 gactgccttt ttgaacagtt gctagaaatg tagaggtatt gaccatgtat caagatttac
    14221 taagaaaaat agcagaagaa aaaccaaatt ataaccagga ggaaatccag tggttgcttg
    14281 atcatttggg agatccttct ccagaaattc gcgatgacct tgtttttaca agctttgcta
    14341 gagggattca ggaagagcta tttacacagg agcaatttca tttcattgct gaaggggttt
    14401 catctgatgt aggactagat aaagagattg ataaggtagg tctgccaaca cttgaacgtt
    14461 cttttagggc acttgtttat gcaaatctct tgtatgcaga tgccaaccag caatcggttt
    14521 tttatcaggg attacaatta gaaattcgta atgtcttttc aaatcaaggc ttgcactatc
    14581 tttcaaaaga aaaggataca acaggtttct caagtcagta tggttgggtt catgcttttg
    14641 cacatggagc cgatttactg acagaggtgg tctgtcatcc agactttcct ataaacagaa
    14701 ttcatgaagt atttgacata cttggccaac tatttaaaag aatgtcgatt cgctttacag
    14761 atgatgagga ttggcgttta gcaagagtga tctatgaacc tattttacaa gggaagttgg
    14821 agcaagaaca agtagcttct tggataaaaa ctgttgattt tccgatagaa gaaagggagg
    14881 atttttccaa attttccaac tttagatcct gtctggtgga agtctatgtc caacttgacc
    14941 agagaaatag tttacaagat gagttgaaag aagctatcca gtcttttcaa tactagagat
    15001 tagctaatag ttctatgatt ttcaaaaaat ttccaatcaa ttttcttgaa accctttcct
    15061 acttttgata gactagtatc tagtttttga aaaaaggaga aagaaaatga aaaaatttgt
    15121 tgctgagtta atcggtacgt tcatgcttgt gttcgttggg acaggagctg ttgtttttgg
    15181 aaatggtttt gatggccttg gtcaccttgg aatcgccttt gcctttggtt tggcaatcgt
    15241 ggtggcagcc tactcaatcg gaactgtttc aggtgctcac ttgaacccgg ctgtttcgat
    15301 tgctatgttt gtaaacaaac gtttgtcatc ttcagaactt gtaaactaca tccttggtca
    15361 ggttgttgga gctttcatcg cttctggcgc tgtcttcttc ctcttggcta actcaggtat
    15421 gtcaactgct agtcttggtg aaaatgcctt ggcaaacggt gtcactgtct ttggtggttt
    15481 cttgtttgaa gtcatcgcaa ctttcttgtt tgtattggtt atcatgactg tgacttcaga
    15541 aagcaagggc aatggcccga ttgctggttt ggtaatcggt ttgtcattga tggcgatgat
    15601 tcttgtcgga ttgaagatta ctggactttc agtaaaccca gctcgtagct tggcaccagc
    15661 tgtcttggta ggcggcgcag cccttcaaca agtttggatt ttcatccttg caccaatcgc
    15721 tggtggagtt cttgcagccc ttgttgcaaa aaatttcctt ggaacagaag aataattgaa
    15781 actcaaaaag ccttgctcct catcttgagg aacagggctt tttcgtatga tactcttcga
    15841 aaatctcttc aaaccacgtc agcttcacct tgtcgtaggt atggtt
//
LOCUS       DAVKJU010000049        15654 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVKJU010000049 DAVKJU010000000
VERSION     DAVKJU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15654
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.049"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(107..724)
                     /locus_tag="VW191_001494"
     CDS             complement(107..724)
                     /locus_tag="VW191_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEU9624241.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLYLKSRRK"
     gene            complement(714..1487)
                     /locus_tag="VW191_001495"
     CDS             complement(714..1487)
                     /locus_tag="VW191_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEU9624242.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLLIAPTHVVSSLAEWDFDEN"
     gene            complement(1498..2235)
                     /locus_tag="VW191_001496"
     CDS             complement(1498..2235)
                     /locus_tag="VW191_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEU9624243.1"
                     /translation="MCLTYQMKIKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(2240..3370)
                     /locus_tag="VW191_001497"
     CDS             complement(2240..3370)
                     /locus_tag="VW191_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEU9624244.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(3454..3843)
                     /locus_tag="VW191_001498"
     CDS             complement(3454..3843)
                     /locus_tag="VW191_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU9624245.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(3859..4242)
                     /locus_tag="VW191_001499"
     CDS             complement(3859..4242)
                     /locus_tag="VW191_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEU9624246.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(4243..5031)
                     /locus_tag="VW191_001500"
     CDS             complement(4243..5031)
                     /locus_tag="VW191_001500"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU9624247.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFSLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(5024..5959)
                     /locus_tag="VW191_001501"
     CDS             complement(5024..5959)
                     /locus_tag="VW191_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEU9624248.1"
                     /translation="MSVLVKEVIEKLRLDIVYGELELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(6089..6265)
                     /locus_tag="VW191_001502"
     CDS             complement(6089..6265)
                     /locus_tag="VW191_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU9624249.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(6405..7112)
                     /locus_tag="VW191_001503"
     CDS             complement(6405..7112)
                     /locus_tag="VW191_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEU9624250.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESNLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            7263..8291
                     /gene="queA"
                     /locus_tag="VW191_001504"
     CDS             7263..8291
                     /gene="queA"
                     /locus_tag="VW191_001504"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU9624251.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGSHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQRIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHSIQEHYRFFS
                     FGDAMFIY"
     gene            complement(8840..9967)
                     /locus_tag="VW191_001505"
     CDS             complement(8840..9967)
                     /locus_tag="VW191_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9624252.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGSIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEKVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFTTKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKGQEVGEIG
                     KITLTTIYAVDERDGTIEETTSRQITKEMVKRRIRRGTREPEKVVVPEQSSIPSYPVS
                     VTSNQGTDVAVEPAKAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGS
                     MKVNQWFQVGGKWYYVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(10683..11234)
                     /locus_tag="VW191_001506"
     CDS             complement(10683..11234)
                     /locus_tag="VW191_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624253.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(11481..12305)
                     /gene="nadE"
                     /locus_tag="VW191_001507"
     CDS             complement(11481..12305)
                     /gene="nadE"
                     /locus_tag="VW191_001507"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEU9624254.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAEMGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(12302..13762)
                     /locus_tag="VW191_001508"
     CDS             complement(12302..13762)
                     /locus_tag="VW191_001508"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEU9624255.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(13878..14366)
                     /locus_tag="VW191_001509"
     CDS             complement(13878..14366)
                     /locus_tag="VW191_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624256.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(14356..14565)
                     /locus_tag="VW191_001510"
     CDS             complement(14356..14565)
                     /locus_tag="VW191_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9624257.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(14950..15402)
                     /locus_tag="VW191_001511"
     CDS             complement(14950..15402)
                     /locus_tag="VW191_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624258.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVASNTMSISQYLVLITTIILLYFFTLKKSKKYSF"
BASE COUNT         4692 a         3485 c         2801 g         4676 t
ORIGIN      
        1 ggctttttgc gaacgattga cacaattgga tttggttaat tcactcttaa cgatggtttt
       61 aaacgatata tatttttata tatgtaaatt aaaaacatct ttcctttcac ttcctacgac
      121 ttttcagata cagatagcca aagaagtttt catagagggc aaaaaagagg aggaaggcat
      181 gaagaaagaa ggtctctggc aaaatcataa taacaggatc cttggctgga tcaaaaagcc
      241 aggtatcatc tcccacaaag agaatttgat ggaaaagagt aaagaattgg tcaaaaccaa
      301 tcaaaactcc cccaagtcca atcatcacag gtaagactac tagagccagg agactttttc
      361 gataaagaga caaaaagtcc tttttcacaa tcctattgac aaagacatag aaacttggca
      421 gtgtcactag agctactagc tgaaccaaat gaaagagatt cttgaccact gcgaaatggt
      481 gcagaccagc tgctgacgaa cgaaaatcag gcatctgtaa gacctgacta aaaggattgg
      541 tcagataatt catcaagata tgaaaattgt attgaatggt ttctggtttt agatagactc
      601 gattcgttaa gtttagccac tgaatctcca taggatagaa aatccaagcc agataaatgg
      661 tcagaaggat tgagagggag aggagaaaga gcatagagcc ccaaaaggtc aatttagttt
      721 tcatcaaaat cccactccgc aaggctagaa accacatgtg tcggtgcgat tagcaggcca
      781 gctacttctt ctgccttagt aaaacctgtc gtcaccaaga gcgttggaat gccattgtca
      841 atcccagccc ggatatcagt caaataattg tccccaacca tgattaactc ttcacgttcc
      901 aaacctaagt gctcaaccgc cttgtccata atgatggcat ttggttttcc gatataaacc
      961 ggcttcactc gtgtcgctac ttcaagcagc gtaatcagtg agccagcacc tggcaaaaga
     1021 ccacgttccg tcgggatgtt gaggtcagga ttggttccga taaaatgggc acccttttga
     1081 atagcaagag ttgctgtggc aaatttttca tagtcgactt gccaatccag accaactacc
     1141 acgtaggcag ggttttcctt gtcttccaca taaccagccg ccttgatggc ttccttgagt
     1201 cctgcttctc cgacgacata gacagtcttt tcaagcccca aatcattcat atagtcgatg
     1261 gttgccaaag tcgctgtgta gacagtcgat agaggcgtat cgatattaaa attctgagcc
     1321 aacatctcct taacactctc tggagtgcgg gttgtattgt tggttacaaa gagataggga
     1381 atgtcccgct tttgcaattc atgaacaaaa gtctctccag cagggattcg gtctttcccc
     1441 ttataaatgg ttccgtctaa atcaattaaa tagcctttat atttcatcta tttctcccta
     1501 aacctttttt atttcttgcc aagtaatgat tgcttgggca ttgataaccc catcacttgt
     1561 aatttcatgc ttgctttcca gtccagtccg ttcaacagcc gatgtaatca ccccacctgg
     1621 tcgaacttcc ttgacatact tgaggttgat tttcttggga atatagtggg tcaaaaaatc
     1681 cgctcccatg acctcaaaaa tccagtccaa gtatttactg ttattgacat gaccattcat
     1741 atccaagtcg taaaaacgaa catggtaatc cttgctgatc ggctcttcca aggattcata
     1801 cttcggtcca cggataagtt ttttatcaaa atcagactgg taaggagcca caatctcagg
     1861 ttcaacagca tggacttttc gactgtcgcg gtccatgaga acaaaggtcg ccatcatatg
     1921 gatgagctcc tgccccgctt cattataaat agtaaagcga cggtagcaaa aaagtcgatt
     1981 gtagctcaag gcttccgttt cgatggtaat ttcttccgca aaacgaggca aacgaaccac
     2041 ctcaatatca tattctgcga taatccagac cagattatag tcttccaaaa tggccttatc
     2101 actaactccc agttcaatcg attgcatccc tgaaacttgc agtgacagca aaatcacatc
     2161 tggaagtttg atatgaccgt tcatatcagc catatcaaaa ggaattttta ttttcatttg
     2221 ataagttaag cacatgatcc tactccaaaa taaatcgttc tgctacagta tctcccaaaa
     2281 agagacctct ctttgtcatg cgaacgtggt caccctcaat ctgcatgagg ccttgttgaa
     2341 ccaaatctct gacaatttct ccataaagtc cagcaaaaga ctgtccaaat ttttcctcaa
     2401 atcgcgccat ggaaaccccg gatttcttgc ggagtcccaa gaacatttct tcttccattt
     2461 gctccttttg actcaggtga tcttctgtaa tacaagcatt gccttcctca accgcactga
     2521 gataatgacg aatgggacca tgatttttat agcgtactcc attgacataa ccagatgccc
     2581 ctgcaccaat accatagtat tcagcattgt cccagtacat gagattgtga cgactttcaa
     2641 agccgggttt ggagaaatta gaaatctcat aatgctcaaa acccgctcgc tccagctctg
     2701 caatgatgta ctcaaacatc tccgcttcta gttcctcctt aggcagaggc aatttcccac
     2761 gtcgcatccg gttcataaag accgtatggt tttctaaaat caaactatac aaactcatgt
     2821 ggggaatatc cagtccaatg gctttagcca cattttcctt tacttgctcc atggtctgac
     2881 caggcagagc ataaatcaaa tcaatggaga tgttgtcaaa accagccagt ttcaggcgat
     2941 cgatattttc ataaatatcc ttctccaaat gactgcgccc aatctttttc aacatcttat
     3001 catcaaaggt ctggacacct agcgaaacac gattgacagc cgaatttttc aaaacagcta
     3061 tcttatccgc atccaaatcg cctggattgg cttcaatggt caactcttcc aagacagaca
     3121 agtccaagtt tttagtcaag ccattcagta acacctccag ttgcggagcc gacagggctg
     3181 tcggtgttcc accaccgata taaagggttg acaacttttc aatatcataa gaacgaaact
     3241 cttccagcag atgctctaaa tagctgtcga ctggctgatt tttgatgaag acctttgaaa
     3301 aatcacaata ataacaaatc tgggtacaaa atgggatgtg cacataggct gacgttggtt
     3361 ttttctgcat agtaattatt ataccacaaa gactagattc cagataaaaa tcaccatccc
     3421 cagatacata gtccgtccgg agatggtgat ggtttattct tctgttatat caatcacaat
     3481 ctcttctgag tcatcaagag cttcggcttt ttcttgccat tgctccttga gattatttaa
     3541 ttgatttttt gatgcttctg tcgcttgaaa agcataggat ttagcttgag caagtatact
     3601 gtccacagtg atttcacctg actcaacctg ttcttttgtt ttcagaacaa aatctgtagc
     3661 ctgctcctta acttctgtca gtttttcaca gacttgctcc ttggcatact ccggatcttc
     3721 tctcaaatca tctagaaaat cttgagcctg actgcaaact tgtttgccct tatcacttgt
     3781 taaaaacaag gcaagagctg cacctgaaac ggttcctaaa aggattgagg ataatttacc
     3841 cataaggatt ctcctttttt attttttgaa aaatttactt gcaagacgaa gagctgacag
     3901 acttgcacca gtcttgagtg tttttgaacc agctgatgaa gctttcttgc tcaagacacg
     3961 cgcatggtca ttgaggtctg aaacagatag agataaatct gcaacagcac tgaagagtgg
     4021 atcaatcgta gccaccttga cattgatatc atctgccaag acattgacct tagccaacaa
     4081 ctcattggtg tgatgcaagg tcacatccac atctgaagtc aaggttttaa tcgtcttttc
     4141 tgtttcatcg atgacacgac caagcttttg tacagtaatg atcagataga ccaaaaagac
     4201 aatcaaagct agggcaacaa gaatatatgc aacttctaac atttagtttt cctcctctgt
     4261 aatatagtaa ggggccttct ttcgattttg ataaataacg atcattatac cgagaccgat
     4321 aaagacaact gacagccatt gggacactcg aaggctgaag aacatgagac tatctgttcg
     4381 cataccttcg atgaccatac gaccgaaacc ataccaaatc aagtaaaagg ccgtgatatg
     4441 acctcgtctg agactcttcc atttccgtct aaaaatcaga atcaaggcaa agccaagcag
     4501 attccataga gactcataaa ggaaagtcgg ttgacggtag ctcccctcaa tatacatctg
     4561 gtcacggata aagccaggta gataatccag attatccact gttgcaccat aagcttcttg
     4621 gttaaagaaa ttaccccaac gccccaaact ttgagcaatc ataacgctag gcgccgcaat
     4681 atctagaaaa tcccaagtat tgatgagttt acggtcagca aagatataga gcacaagagc
     4741 cccagttatc aaaccaccgt aaatggccaa accaccattc caaatggcaa aaatctctcc
     4801 taaattctga ctatagtaat caaatcggaa aataacatag tagagacgag ctcctaaaat
     4861 agccaaggga aaggctacta agataaaatc taaaatatcg tctggtatga tcttctttct
     4921 aggtgcttct ttcatggtca aataaaccgc aagaatcaag cctgtcacaa tacataaggc
     4981 ataccaacga atggctaggg gtcctagttg aatagcaatt ggatcaagca ttttgcacct
     5041 catttcgagc tatgagactt gtcagtcgtt cgtcgaacaa acgggtagca tcaaagccca
     5101 tttccttggc acgataattc atggcagctg cctcaatcac aacagagata ttacgacctg
     5161 ttttaactgg aatacgaata cgaggaatgg ctacgccaga aacttcaagt tcctctgcat
     5221 tgtttccaag acgatcaaag gtcttatgcg tatcgtaatt ttccaaatag acagcaagct
     5281 gaacctgtga agaatccttg acagcactcg caccgtagag actcataaca tcgataatac
     5341 caaccccacg aatttcaatc aagtgtttca aaatttcagc tggttcaccc cagagagtaa
     5401 tctcatcctt ggcaaagata tcgacacggt catcggctac caaacggtga ccacgtttga
     5461 caagctcaag acctgtctcg ctcttaccaa ttccactatc tccctgaatc aagacgccca
     5521 tcccataaat atccatcaag acaccgtgca cactggtacg ttctgccaaa cgagaatcca
     5581 gatagctaga taactctcca gataaacgac tggtagctgc acggctggtt aaaatagcaa
     5641 tcttacattc tctagcagcc tttaacatct cctctggaac caccaaacca cgggcaacaa
     5701 tgactgctgg tgtctcaggt agaaacattt ttttcaaaac ttcataacgg ctgttggaag
     5761 gcatgctgat cagataagac cactccttca tccccaaaag ttgaatccgc tctggtgtat
     5821 agtagtcaaa atagcctgtc atttcaagac caggtcgcgt aatatccgct gtattgattt
     5881 ccttttcaag caattctagt tcaccataga caatatctag tctaagcttt tcaatcactt
     5941 cttttactaa aaccgacatc atttcctcct tcaaatgaat tcttctctct attatatcaa
     6001 attgtaggag gaagtttcag atttttgcag gctttggagt atttatttaa aagaaaaagg
     6061 actagatttc tctagccttt catttcaatt agaatttttt acgtttacga gctacttctg
     6121 atttacgttt acgttttaca gaaggttttt catagaattc acgtttgcgt gtttcttgaa
     6181 gagtaccagc tttagtaacc gcacgtttga aacggcgaag tgcgtcgtca agagattcat
     6241 tcttacgtac tactgtttta gacatttttt tcactccctt caagttcaat atctatttca
     6301 ttctacacgt aaaaggaata attgtcaagc aaaaatgcgg tttaattttg attttttctt
     6361 ccatttatac aagagttgaa agcgttcaat agaacattgc ttttttattt ttcgagtaag
     6421 ctaagcgctt cagcatctgc gatgatggtt acatcagggt gattttggag gctacttgca
     6481 ggtaggttct cagtcactgg gccagatact gttccggcaa tggcttctgc tttcgactca
     6541 ccataagcaa aaagaataat agacttggca tccaaaatgt ttttaatccc cattgaaata
     6601 gcttgggttg ggacgtcttc aatcttggca aagaagcgag cattggcttc gatagtagac
     6661 tggtcaagtt ctactagatg cgtttgactg tcaaatggag taccaggctc attaaagccg
     6721 atatgcccat tgcgaccgat tcccaagatt tggaggtcaa ctggatggtc agccaaaatt
     6781 tggttgtagc gttcaacttc agcttcagca ttgtccttaa caccacgagg caagaaactt
     6841 tctttaaatg gtttttggtt gaacaagttt tcttgcatga agtaacgata agactgtgga
     6901 ttgtccccat caaggcccac atactcatca aggttgacac tggttagatt tgaaaaatca
     6961 aggttactct caacaatttc cttgtaaaat tcaagtgggc tacttcctgt cgcaagtcct
     7021 agagtttgag ctccattagc caatttttcc ttcaaaatct caaaagcaac ttttccacct
     7081 tgaacttggt tttcaacttt aataactttc atcttttcat cctccatttc tgtctatatt
     7141 tattatatgg tatagaccaa tttttgtcaa gtgaaaacga tttaatttct aaaaaaagag
     7201 cgtccaaact tgaaacgtgt tataatggat aggattagaa cttagaaaga atgaacagat
     7261 atatgaatac agctgatttt gatttccact tgccagagga attgattgcc caaacgcccc
     7321 ttgaaaaacg tgatgcctcc aaactcctca tcgtcaaccg tgagacagga gaaatgcaag
     7381 ataaacactt ccactctatt attgatatgc tggaacctgg tgatgccctt gtcatgaacg
     7441 acacccgagt tctccctgcc cgcctctatg gtcaaaaagt ggagacagga agtcatgtgg
     7501 aacttctcct ccttaagaac actagtggag acgagtggga agttctggct aaacctgcca
     7561 aacgcctcaa ggtcggtact cgtatcagct ttggtgatgg ccgcctcagc gctgtcgtta
     7621 cagaagaatt gacccacggg ggacgcattg tccgctttga ataccaaaga attttcctag
     7681 aagtcttgga aagtctggga gaaatgcctc tgccacctta tatccacgaa aaattagatg
     7741 accgtgaacg ttatcaaacc gtctacgcca aggaaagcgg ctctgctgca gcaccgactg
     7801 ctggtcttca cttcaccaaa gaactgctgg cagaaatcca agctaagggt gtccatctag
     7861 tctatctgac tctccatgtc ggactcggaa cctttagacc tgtttctgtg gataatctgg
     7921 acgaacacga aatgcactca gagttctatc aactttctga ggaagctgcg gccacccttc
     7981 gctctgtcaa agaaaatggt ggtcgtgtca tcgctgtcgg aaccacttct atccgtacct
     8041 tggaaactat tggttccaag tttgatgggc aaatccaagc agattctggt tggaccaata
     8101 tctttatcaa acctgggtat gagtggaagg tcgtggatgc cttctcaacc aacttccacc
     8161 tgccaaaatc aactctggtc atgttggttt ctgcctttgc aggccgtgaa ttagtcttag
     8221 atgcctacca ccattccatc caagaacact accgcttctt cagttttggt gacgccatgt
     8281 ttatttattg agaaagaatt tctctaaatc ttctaatacc aataaatcgc taagatatta
     8341 tttcaaagaa catctacaat tgaaactcta gctagctgta gaagacgcct agtacattga
     8401 aattaaaatg caagacacat gagtagcacc atatttgttg ctcttatctg tcctcgcaag
     8461 agactattat gagttatttc agaatcattc actactttga ccctgactct ccttagtctc
     8521 aaaatcaaag acttatactc ttcaaaaatc tcttcaaacc gcgtcaacgt catcttggat
     8581 tatatatgtg actgacttcg tcagttctat ctacaacctc aaagcagtac tttgagcaac
     8641 ctgcgactag ttttctagtt tgctctttga ttttcattga gtattaaaca aaaagtgaac
     8701 aaagctgaat tctaatgtac agaagactag gcttgttcac ttttttatag tcgctataag
     8761 atgaccttat ctatagcttt ttatatataa ttatatattc agacatacta ttatcaattt
     8821 tgtcgcaggg aggaatctgt taacgcaccc attcaccatt atcattgact ctatagccat
     8881 ctatacttgt attgaccgct aactcacccg atgtatttac ataataccat ttaccaccaa
     8941 cttggaacca ttgattgact ttcatagaac cgttgctgtt gaggtagtac catgaaccat
     9001 taacttgtac ccaacctgtt gccatggaac catcagtatt ataaaaatac cacataccat
     9061 tttcttgttt ccagcctgtt gttggagcaa ctgctttagc tggttctact gctacatctg
     9121 ttccttggtt agatgtaaca gatacaggat acgaaggaat agatgattgc tcaggaacaa
     9181 caactttttc aggttctctc gtccctctcc ttatacgtct ttttaccatc tctttagtaa
     9241 tttgacgaga agtagtttct tcaattgttc catcacgttc atctacagca tagattgtag
     9301 taagagtaat tttaccaatt tctcctactt cttgaccttt atcaagagtt gggtcatcga
     9361 gatattctgt ttcgattgga atttcttgga caagaacttg gggcttggtt ccttttttaa
     9421 caactcttgt ttgagagtct tctttttgac ttaaagtact ctcagttact tgtccactct
     9481 ttccatctac atcataagtt atcgttgtaa ctgttttccc attctttcct agagtaatct
     9541 cttgctcctg tcctgcagaa aggtcattgt ctgcttcata tttagtagta aatggaacaa
     9601 gaacttcttc aaccttgctt ttagctggaa ctttgataac tgtatccgtg gcttcttttc
     9661 tatcaacagt aaccttttcg gtaacatgac cagtctctgg attaacatcg taggtccttg
     9721 tcgtagttac atagccatcc tctccatcaa ttgtaacagg attttcacta cggtcttttg
     9781 tttcatcttt ttcataacga attcgcgtac ttgaaatttt cttggttact accttaggtt
     9841 tagtcgctac ttttacaata atactcccat tgtcagcgtc atcatactct attccctctt
     9901 ctttatctct agtatcatct ctgacatatt gaatcccatc agcagcatga acaaaacttg
     9961 tattcagatt cctcctaaaa ataaagttag cccgattacc gcagaaccaa aaatctttcc
    10021 gagtttacgt aatgcatagc gcttattagt attagatttt gccattacat cctacttcta
    10081 gtatagcatc ttttctatca aacgttaaac agtatacttt atatatcaaa tagacttaga
    10141 atgatatatt gattattgaa ctaacacttt aactatatcg taatcaatct catatataaa
    10201 ggattgcaga catcttatct aaatacatgc taatatattt agatacaaac attccaactt
    10261 gataattttc actcatcttt catcattcct tatacaacta tgcagtataa atagaatagt
    10321 tttctcatca gaatgagact attttaatat tagataattt gatagaaaaa aacataaata
    10381 aaattggttg aatttaattc cctaactcga tagaagattc tattttaaat ttaacaacag
    10441 ctaggattac actataaaaa gcacattacc acctgttcga ctggagaatc tagtcgatta
    10501 gctccctctt agttacaagc ttaaagcgat tttttaacta atactaaacc agattaaaga
    10561 aggaactaga acatctgtct tgtactaatc aatatgctat tttaaaattg caaataaact
    10621 tggaatgcta attattatat ttacaataaa tagttgactt cgatagaaac agaaaataga
    10681 attcaacatt cttccctact tataccataa taaataagat ccgcaagata gccttttctc
    10741 tctacaccat taacaatggt ttgtagatag gacattccag ccttttccat gacacgacct
    10801 gaagctgggt tgagactggc ataacgtgct ctgacctttt gaaaacctgc ttgagtaaaa
    10861 caaaagtcca agacagcttt caaggcctct gtcatcatac catgtcccca gtaagccttg
    10921 cctagcacat agccgatttc acagcttaaa tcagcctcgt ctatcttaac gatgctgata
    10981 tctcctatca cttgctctgg gttttctttt agacaaatgg cccatttata atagttgaga
    11041 ttagtatagg aagcaaccca attgcgaatc gagtttcgag tgatttcgac atcaggatgg
    11101 ggatcccagg taacataggt cagattctca gcggatgaag cccaattttg aaacatggct
    11161 tctgcatcac tctccacaaa tcttcttaaa atcaaacgtt ctgtctgtaa tatttgcgta
    11221 ccgatagatt tcatgatgct acctcacttc cttatttcta tgctcgtagt aaaaagactc
    11281 aactcagtaa cagctagaaa acgagtttgt cttctaattt cttgacgcag ttgctgcatg
    11341 gtttgaaatg cgctctagca atctttttca aacctagtca tcgttgcagg ggtaagacga
    11401 tgaaatcgaa ctcttcgagt taccgaatct gcctcatttt cttatttcta aactcggggt
    11461 tcaagatccc ccggaccttt ttactcccaa aagtcatcaa atacggtgat gggtaagtgg
    11521 cgtttgtgtt ggcctttgtg ccaccagttt tcaatggtcg cttgagcttc tgggctgatt
    11581 gttttgcctt ctaggtaatc gtcaatctct gcataggtga ctccaagtgc gacttcgtca
    11641 gctaggcctg gtttgtcttc ttctaggtct gccgttggga ttttttcata aagggctggc
    11701 tctgcgccaa gtttctgcaa gagttgtttt ccttggcgtt tattgaggcg gtaaagaggg
    11761 agaatatccg caccgccgtc accaaacttg gtaaagaaac ctgtgatatt ttccgcggcg
    11821 tggtctgttc caatgaccgc tccgctatgg gaaccagcaa gggcatactg agcaatcata
    11881 cggcaacgtg ccttgatatt ccccttgttg aagtctgaaa caggactacc tgtcgcttca
    11941 actgcagctg tcatggcatc agctgattcc ttgatattca caaccaagct gacatctggc
    12001 tggatgaagg ctagggcttt ttgagcatct gcttcatcag cttgcactcc gtatggcagg
    12061 cggacagcga taaatttgta gctatcgtct cccatttcag ctcgcagttc ttccatagct
    12121 aattgcgcca aacgtcctgc caaggttgaa tcttgtcccc cagaaatccc tagtacaaag
    12181 gtttttagga agggatgttt tttcagatat ctttttaaga aatcaataga acgacggatt
    12241 tcttcctggg catcaatcac tggtttgaca cccagctctt ggataatcgt ttcttgcaaa
    12301 ctcattcttc ttctccttca ccaagggctt ccttgcgcat cttatcaatc aagtccatct
    12361 tatcttgcca tacatcacgc gccaaatcca ctggatagtg ctgcggattg agcacacgct
    12421 tatactcatc ccacaacttg tcaaattcct tacgggcata atcctgaatg tcagtcaaac
    12481 taggcaagtt gtaaactaat ataccttctt tgaagatatc caccaagaga ggaacggcat
    12541 caaaattacg aaccgtcttc ttgatgtatg tataggtcgg atggaacatc ttgatttctg
    12601 tcatgtcgct aatatccaca ccatcataag tgatgtagtc accttctgac ttgccttttt
    12661 cacgactggt aatgcgccac acctgcttct tacctggcgt cgacactttt tccgcattat
    12721 tagacagctt aatcgtattg cgcatctgac cagtttcatc ttcgattgca acaatcttgt
    12781 aaaccgcccc aagagctggc tggtcatagg ctgtaatcag cttggtaccc acgccccaga
    12841 catcaatctt ggccttttgc atcttgaggt tgaggatagt attttcgtcc aaatcattag
    12901 aagcataaat cttagcctct gtaaatccag cctcgtccag ttgctgacgg actttcttag
    12961 aaatgtaggc aatatcccca gagtcaatcc gcacacccat aaagttaatc tgatcaccca
    13021 gctcacgcgc cacctgaatg gcagctggta caccgatgcg aagggtgtca taggtatcca
    13081 caagaaagac acaatttttg tgggtcgcag cgtaagcctt gaaagcttca tagtcattgc
    13141 cataaacctg taccaaggca tgggcatggg ttcccaaaac aggaatgtca aagagcttac
    13201 ccgcacgcac gttgctggtt ccattggcgc caccaatcac agctgcgcgt gttccccaga
    13261 tggccgcatc catttcttga gcccgacgtg tcccaaactc catcaagggt tcatcttcga
    13321 taaccgaacg aatacgagct gccttcgtcg ccaccaaggt ctggtagttg acgatgttca
    13381 aaagagccgt ttcgaccaac tgacattggg ctagaggtcc ttccacctgc acaatcggtt
    13441 cattagcaaa aaccaaatcc ccttcttggg cagaacgaac ggtcaactcc aacttgaaat
    13501 tgcgaaggta atccaagaac gccccatgat aaccaagcga ctccaaatag gctatatcac
    13561 tatctgaaaa acgcaagtct tcaagatagt tcacaattct ttccaaacct gcaaaaaccg
    13621 catagccgtt cttaaaaggc tgttggcgga aatacacctc aaagaccgcc ttcttattgt
    13681 gaatcccttg gtcaaagtaa acctgcatca tgttgatctg gtacaagtcc gtgtgcaatg
    13741 tcaaactatc atctggatac atacttttcc tacttcctta gctagaaacc catgaaaatt
    13801 ttcaagaact ttcatgtatt ccaataaatt agtactatta tatcacattt tagctggatt
    13861 gagaaaagag taacaagcta ttctccactc tccaattcat ccatatcttg ttcaaatttt
    13921 ttctgagccc attcgccata gctcttaaga ccaagattgc caataaagac ccacggaagg
    13981 taaatgacat aagtaatgac ccaagcagac aggtatttaa aattcaaagg attgtgctga
    14041 taaatttcta tgttgaattg ataattctgc aacatcaaaa gagccgtaat agccaaggtt
    14101 aggaaaaaac aacccaaaat cgtaaaatga aaacgactat agtaggtcac tcccagataa
    14161 cgggcacgat tgaaaaagta aaatgtccct atgatgataa cgattagcag catattagaa
    14221 ttaaaaaggc ttggtgctaa tactgaaatg atataagata ggagcgacaa agcaatgcag
    14281 atatagaaac tttcagagcc cgctttattg aacagttgtt cttctctttc gtctagtaat
    14341 tgataataat aaaatctatt tttcatcttc ttcctcccaa aatagttggt ctagggtttt
    14401 ccctaaacat ctgcaaatag actggcagag cgagagactg ggattgtatt ttcccgcctc
    14461 tatcaaacca atagtctggc gtgtcacccc gacagcctct gccagttgac cttgtgttaa
    14521 atcacgctct acccgagcta attttaattt taaattttta gccaccttcg tcctccttat
    14581 agttttaata ctcatctact cttaaaaaat ccaaaaccaa cacaagctat cagctattta
    14641 ttataacccc attaatttgc actccagtat tatcttcagc cgaaaatcag tattttaggt
    14701 tgtttgaaat aaacttccta gtttactctt ttatttttat ttagtattag ctttagtagg
    14761 atcttttaaa cgagttttta ttgcttatat ttcttgttta gtttagttgg tttcatatca
    14821 ctattatata atgcttttgg aaatgagcct agtataagtc aaaaacgtgt tgaatagtga
    14881 aagcaggagc ataccaagat gactgatttt ttactgtatt ttaggtatgc aaaataatac
    14941 taaatgaaac taaaatgaat acttcttgct tttcttgaga gtgaaaaagt acaataaaat
    15001 gattgtagtg attagcacaa gatactgact aatagacatc gtatttgaag ccacaaatac
    15061 aggtaaagaa taaaaaggaa gaagttcaat tgctttgata aacaaatcat tactagtatt
    15121 catcatcatc gtatttaata tattagggat aaagagtagt aaagctacta aaataccaaa
    15181 taccaagcca cttttcaatt tgtataaata gaaaatgtaa gataatagaa aataaaacag
    15241 gactaacaaa attgtattga ctacaatagc tactataaaa tctaatccta aatcacctgg
    15301 aatacccaat ttacttacta tgactagtaa cacaatgcca ttcaaagcat aaataatgga
    15361 aactgagatg gcatatagaa aattagacaa gacgtaaatc atccgattct tctgttgatt
    15421 ataaaacaaa cttaccgtat tgtgggaaaa atctctacta atgactcgat tagcatgtat
    15481 aacagccatt aacataccga gcatagcata aaagtttgaa atatgcttaa taccaagcga
    15541 tgtcccttga gtctttagaa taaagaatgt aagcaatatc ggcggtataa acgagataag
    15601 caaactgaga tatattacgc ctgatgaata taaatttctt atattttcct taag
//
LOCUS       DAVKJU010000050        15281 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVKJU010000050 DAVKJU010000000
VERSION     DAVKJU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.050"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            203..1990
                     /locus_tag="VW191_001512"
     CDS             203..1990
                     /locus_tag="VW191_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEU9624259.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1987..2463
                     /locus_tag="VW191_001513"
     CDS             1987..2463
                     /locus_tag="VW191_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEU9624260.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPIEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2490..3395
                     /locus_tag="VW191_001514"
     CDS             2490..3395
                     /locus_tag="VW191_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEU9624261.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERYDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQHVVDLVTEYQWIADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3382..4197
                     /locus_tag="VW191_001515"
     CDS             3382..4197
                     /locus_tag="VW191_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9624262.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATMINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFAFGM"
     gene            4204..4608
                     /locus_tag="VW191_001516"
     CDS             4204..4608
                     /locus_tag="VW191_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9624263.1"
                     /translation="MTKSLILVSHGRFCEELKGSTEMIMGPQDNIYTVALLPEDGPED
                     FTAKFEAAIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIELYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4708..5874
                     /locus_tag="VW191_001517"
     CDS             4708..5874
                     /locus_tag="VW191_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU9624264.1"
                     /translation="MLHYTKEDLIELGAEITTREIYQQPDVWKEAFESYQEKREEIAA
                     FLQGIADKHDYIKVILTGAGTSAYVGDTLVPYFKEVYDERKWNFNAIATTDIVANPET
                     YLKKDVATVLVSFARSGNSPESVATVDLAKALVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAASNDAGFAMTSSFTSMMLTALLVFDPTEFAVKAERFEVVSSLARKVLDNA
                     EDVKELVDLDFNRVIYLGAGPYFGLSHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INEDTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            5990..7027
                     /locus_tag="VW191_001518"
     CDS             5990..7027
                     /locus_tag="VW191_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEU9624265.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7235..8089
                     /locus_tag="VW191_001519"
     CDS             7235..8089
                     /locus_tag="VW191_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU9624266.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            8226..8483
                     /locus_tag="VW191_001520"
     CDS             8226..8483
                     /locus_tag="VW191_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEU9624267.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVKSSV
                     ECVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(8525..8887)
                     /locus_tag="VW191_001521"
     CDS             complement(8525..8887)
                     /locus_tag="VW191_001521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624268.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFWGSVHSTPDTMR
                     FFNFRDFLQLLLTHIKLF"
     gene            complement(8941..9237)
                     /locus_tag="VW191_001522"
     CDS             complement(8941..9237)
                     /locus_tag="VW191_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU9624269.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(9227..9388)
                     /locus_tag="VW191_001523"
     CDS             complement(9227..9388)
                     /locus_tag="VW191_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEU9624270.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            9458..10222
                     /locus_tag="VW191_001524"
     CDS             9458..10222
                     /locus_tag="VW191_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEU9624271.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(10462..10614)
                     /locus_tag="VW191_001525"
     CDS             complement(10462..10614)
                     /locus_tag="VW191_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624272.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            10832..12211
                     /locus_tag="VW191_001526"
     CDS             10832..12211
                     /locus_tag="VW191_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEU9624273.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            12225..12890
                     /locus_tag="VW191_001527"
     CDS             12225..12890
                     /locus_tag="VW191_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU9624274.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            13004..13099
                     /locus_tag="VW191_001528"
                     /pseudo
     CDS             13004..13099
                     /locus_tag="VW191_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            13380..13829
                     /locus_tag="VW191_001529"
     CDS             13380..13829
                     /locus_tag="VW191_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9624275.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLMIGGEQVEILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGVVNCGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            13986..14396
                     /locus_tag="VW191_001530"
     CDS             13986..14396
                     /locus_tag="VW191_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEU9624276.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            14512..>15281
                     /gene="pavB"
                     /locus_tag="VW191_001531"
     CDS             14512..>15281
                     /gene="pavB"
                     /locus_tag="VW191_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEU9624277.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRA"
BASE COUNT         4291 a         2875 c         3496 g         4619 t
ORIGIN      
        1 aaaattattt taggtactat attccaaatt taaagagcgt ttgaaatggg tctaaaaatg
       61 tttgaaacta attgaatgtg tgtataatag taaaaatgca gtaaatatcc cgacaatttc
      121 tattgacaat tcaaacagat tggtttataa ttaatataac aacaaatgaa agcgcaaact
      181 ttctcggtcg gaaggtagtt ttatgacacg atttgagata cgagatgatt tctatctcga
      241 tggaaaatca tttaagattt tatctggtgc cattcattat tttagggttc ctccagagga
      301 ttggtatcat tcgctctata acttgaaggc tcttggtttt aatacggtag agacttatgt
      361 tgcttggaat ttacacgagc ctcgtgaagg tgagtttcat tttgaaggtg atctggattt
      421 agagaaattt ctccaaatag cgcaggattt gggtctctac gcaattgtgc gtccgtctcc
      481 atttatctgt gcggaatggg aattcggtgg cttaccagct tggctcttga ccaagaacat
      541 gcgaattcgc tcatccgacc cagcatatat cgaggcagtt ggtcgctact atgatcagtt
      601 attgccaaga ctggtgcctc gtttgttgga caatggtggc aatattctca tgatgcaggt
      661 tgaaaatgag tatggttctt acggagaaga taaggcttac ctgagagcga ttcgacagct
      721 aatggaagag tgtggcgtaa cctgtcccct ctttacatca gatggtccat ggcgagctac
      781 tctgaaagct ggaaccttaa ttgaagagga cctctttgta acaggaaact ttggttctaa
      841 ggcaccttac aacttttcgc agatgcagga attctttgat gaacatggta agaaatggcc
      901 actcatgtgt atggagttct gggatggttg gttcaatcgc tggaaagaac cgattatcac
      961 acgggatcct aaggaattgg cagatgcagt tcgagaggtt ttggaacaag gctctatcaa
     1021 tctttacatg ttccacggtg gtacaaactt tggtttcatg aatggttgct cagctcgagg
     1081 aactttggac ctgccacaag ttacgtctta tgattacgat gcccttctgg atgaagaagg
     1141 aaatccaact gctaaatatc ttgcagtcaa gaagatgatg gcaacacatt tttcagagta
     1201 tccgcagttg gaaccactct acaaagagag tatggagttg gatgctattc cactagttga
     1261 aaaagtttct ttgtttgaaa ccttagatag cttgtcaagt cctgtagaaa gtctctatcc
     1321 tcaaaagatg gaggagctgg gacaaagtta tggctaccta ctttatcgaa cagaaacaaa
     1381 ctgggatgca gaagaagaaa gacttcgtat cattgatggt cgagataggg cccagctgta
     1441 tgtcgatggt cagtgggtta aaactcaata tcagacagag attggggaag atatttttta
     1501 tcaaggtaaa aagaaagggc tatctaggtt agatatcttg atagaaaata tggggcgtgt
     1561 caactatggg cataagttct tagcggatac gcaacgtaag ggaattcgga caggggtctg
     1621 taaggatctg catttcttac taaactggaa acactatcca ctcccactag acaatcctga
     1681 gaaaattgat ttttcaaaag gatggactca aggacaacca gccttttacg cttatgactt
     1741 tacagtcgaa gagccaaaag atacttacct agacttgtct gagtttggta agggggttgc
     1801 ctttgtcaat gggcagaatc taggacgttt ttggaacgtt ggcccaactc tctcacttta
     1861 tatccctcat agctatctca aggaaggtgc caaccgcatc attatctttg aaacagaagg
     1921 tcaatataaa gaagagattc atttaactcg taaacctaca ctaaaacata taaaggggga
     1981 aaacttatga caattgtagg atgccgtatc gatggacgtt tgatccacgg acaagtagcc
     2041 aatctttggg ctggaaaact aaatgtttca cgcattatgg ttgtagacga cgaagttgtc
     2101 aacaacgata ttgaaaagag tggtttgaaa cttgcgacac caccaggtgt gaaattgagt
     2161 attttgccaa ttgaaaaagc ggcagccaat attcttgctg gtaagtacga tagccaacgt
     2221 ctctttatcg tggctcgtaa accagaccgc ttccttggct tggtcgaagc aggtgtacca
     2281 cttgaaaccc tcaatgttgg gaatatgtct caaacaccag aaactcgttc tattacacgt
     2341 tctatcaacg tagtagacaa ggatgtggaa gatttccaca aactggcaga aaaaggtgtt
     2401 aaacttactg ctcaaatggt tccaaatgat ccaatttcag actttttgag cttattaaaa
     2461 taggaaaaaa tttttaggag gtcattgtta tgatacaatg gtggcaaatt ttacttctca
     2521 ctttgtactc agcttatcaa atctgtgatg agttgacgat cgtttcatct gcaggttccc
     2581 ctgtatttgc tggtttcatt actggtttaa tcatgggaga tgtgactact ggtttgctta
     2641 tcggtggtaa cttgcaactg ttcgttcttg gggttggtac cttcggtggt gcttctcgta
     2701 tcgacgcaac ttctggtgca gttcttgcga cagccttctc tgtttcacaa ggaattgatg
     2761 caccgcttgc aattactaca atcgctgtac cagtagcagc tctcttgact tacttcgacg
     2821 ttcttggacg tatgactact accttcttcg ctcaccgtgt ggatgctgca atcgaacgct
     2881 atgactataa aggtattgaa cgcaactact tgcttggtgc gattccgtgg gctctatctc
     2941 gtgcccttcc agtcttcttt gcccttgctt ttggtggtgc ctttgtacaa catgtagtag
     3001 acttagtgac agaataccaa tggattgcag atggtttgac actcgcagga cgtatgcttc
     3061 caggtcttgg atttgcaatc ttgcttcgtt accttccagt taaacgtaac cttcactacc
     3121 ttgctatggg atttggtttg acagctatgt tgactgttct ttactcatat gtaacaggtc
     3181 ttggtggcgc tgttgctggt atcgtaggta ctcttcctgc tgaagttgct gaaaaaattg
     3241 gtttcgtgaa caacttcaaa ggtttgtcta tgattggtat ttctatcgta ggtattttcc
     3301 ttgcagtgct tcacttcaaa aatagccaaa aagtagctgt agcagcacct tctacaccat
     3361 cagaaagtgg ggaaatcgaa gatgacgaat tctaattaca aactaacaaa agaagatttt
     3421 aatcaaatca acaaacgtag cttgtttact ttccaattag gttggaacta cgaacgtatg
     3481 caagcttctg gttaccttta catgatcttg cctcaattgc gtaaaatgta tggggatgga
     3541 actcctgaat tgaaagaaat gatgaaagtt catactcaat tcttcaatac ttcaccattc
     3601 ttccatacca ttatcgctgg ttttgacctt gccatggaag aaaaagatgg tgtgggttca
     3661 aaagacgccg ttaatggtat caagacaggt ttgatggggc cattcgctcc tcttggagat
     3721 acaatctttg gttcacttgt acctgccatc atggggtcag tcgcagcaac tatggctatc
     3781 gctggccaac cttggggtat cttcctttgg attgcagttg cagttgctta tgacatcttc
     3841 cgttggaaac agttggaatt tgcttacaaa gaaggggtta accttatcaa caacatgcaa
     3901 agtaccttga cagctttgat tgacgctgca tctgtacttg gtgtcttcat gatgggtgct
     3961 cttgtagcaa caatgatcaa ctttgaaatt tcttacaagt tgccaatcgg tgaaaagatg
     4021 attgatttcc aagacatctt gaaccaaatc ttcccacgtt tgcttccagc aatctttact
     4081 gcctttatct tctggttgct tggtaagaaa ggtatgaact ctactaaagc tatcggtatt
     4141 attatcgtac ttgctttggc tctttctgcc cttggtcact ttgcatttgg aatgtaattc
     4201 cttatgacta aatcattaat tttggtgagc catggtcgct tctgtgagga gcttaaaggt
     4261 agcacagaaa tgatcatggg cccacaagac aatatttaca cagtagctct tcttccagaa
     4321 gatggcccag aagactttac tgcaaaattt gaagctgcta ttgaaggatt ggatgatttc
     4381 ctagtctttg cggatcttct cggtgggaca ccttgtaacg tggtcagtcg tttgatcatg
     4441 gaaggtcgtg acatcgaact ttatgcaggg atgaatctcc caatggtgat tgaatttatc
     4501 aatgcgagcc tgacaggcgc agatgcggac tacaagagcc gtgctgcaga aagcattgtg
     4561 aaagttaatg acctgttagc aggcttcgat gatgacgaag atgaataaga tgtgacaacg
     4621 tgaacatcgt cagaacaact tatatagaat atacatggga atggggctta ctcccattcc
     4681 catatttaat agaaaaagag ggaatcaatg ctacattata caaaagaaga cttgattgaa
     4741 ttgggtgcag aaatcacgac acgtgaaatc taccaacagc ctgatgtatg gaaagaagct
     4801 tttgaatctt atcaagaaaa acgtgaagaa attgcagcct tcctacaagg gattgcggat
     4861 aaacatgact atatcaaggt tattttgaca ggtgctggga cttctgctta tgtgggagat
     4921 accttggtgc cttactttaa ggaagtctat gacgaacgca aatggaattt caatgctatt
     4981 gcgacaactg atatcgttgc caatccagaa acttatttga aaaaagatgt ggcaactgtc
     5041 cttgtgtctt ttgctcgtag tgggaactcg cctgaaagtg tggcgactgt tgatttagca
     5101 aaagccttgg tggatgagct ttaccaagtg acgattactt gtgcagcaga tggtaaattg
     5161 gctcttcaag cccacggcga tgaccgtaat ctattgctct tgcaaccagc tgcttcaaat
     5221 gacgctggat ttgccatgac ttctagcttt acgtctatga tgttgacagc actcttggtc
     5281 tttgatccta cagaatttgc tgtgaaagct gaacgttttg aagttgtatc tagtcttgct
     5341 cgtaaagttt tagataatgc agaagatgtc aaagagcttg ttgacctaga ctttaatcgt
     5401 gtcatctatc taggcgctgg tccttacttc ggcctttctc atgaagcgca acttaagatt
     5461 ttagaattga cagctggtca agttgcaact atgtatgaaa gccctgttgg cttccgtcac
     5521 ggtccaaaat cattgatcaa cgaagataca gtcgttttag tttttggtac aacgacagat
     5581 tatactcgta agtatgattt ggatttggtt cgtgaagttg caggtgatca gattgctcgt
     5641 cgtgttgtgc ttttgagtga tcaagctttt ggtcttgaaa atgtcaaaga agtggcactt
     5701 ggttgtggcg gtgtcttgaa tgatatttac cgtgtcttcc cttacatcgt ttatgcccaa
     5761 ctctttgctt tattgacttc actcaaggta gaaaataaac cagatacacc gtctcctaca
     5821 ggtacagtaa accgtgtagt acaaggtgtc ataattcacg aatatcaaaa gtaagacagt
     5881 gtttatgaat tcttgacaag aggatttgta aattatcaga taaaccatag attgtcagta
     5941 cgctttctat ggtttgtttg cttgagagaa atagtaaaag gagaacagaa tgaaagcata
     6001 cacagagcgt gtatttggaa atgttgaggg tgaggatgtc ttggcctatc gatttgagac
     6061 agacggtggc taccaacttg aggttatgac ttatggtgcg actatcttgc gctatgtcgc
     6121 acctgacaag gctggaaatt ttgccaatgt tatcttggga tttgatgact ttgatagtta
     6181 tgtaggcaat agtcccaagc atggagcaag tgtaggtcct gtagcgggtc gtattgcagg
     6241 tgcgaccttt gagctcaatg gtaagaccta tgaccttgag gttaataatg ctagcaactg
     6301 taatcacagt ggttcaactg gttgggattc cagcttgttt gaagttgaag aagtaagcga
     6361 tcatggcttg actctctaca cagagcgtac agatgggaca ggagggttcc ctggaaatct
     6421 caagatttgg atcagttatc acttggaaga aactggtgcc tatgaaatca gctacaaggt
     6481 aacgaccgat caggatacgc tggtcaatcc aaccaaccac agctatttca acttgtctgg
     6541 tgatttcacg cagacgattg accgtcatgt cttccaacta aacacagagg gcatttactc
     6601 aatcgctcct gacggtgttc ctgccaaaac tccagaagcc aaccgtgatg tggtcaaaca
     6661 cgtctacaat ggtaccttgt tgaaggatat ctttgcagaa gaagatgagc aaatccagct
     6721 ggcatcaggt ttggatcatc catttgccct tcctgcaggc catgacaatg ctggattcct
     6781 ttatgaccaa aattcaggtc gcttcctgct tttcaagaca gaagctcctt gctttgtggt
     6841 ctacacagca aactttgtgg atgaaagtgt catcatagga ggtcagccaa tgctacagca
     6901 caatgggatt gctcttgaag cgcaagcttt accagatgcc attcacagtg accttaaagg
     6961 ccaagtcatt cttaaagctg gtcaaacctt caccagtaag acacgttatg aacttgttgt
     7021 gaaataaaaa aagctggtca aaaagtcttt aaaattaaaa taacgcatat tgtcaggtga
     7081 tgaaaagttt gataatatgc gttttattat gaaatgattt actttatatt atcctgaaat
     7141 ggtgttattt ttgagttatc tggcttgact ttcttgatgg aaattatata atagttgtaa
     7201 caataataat tacaactaga aaagaggacc atttatgacc tatgaataca agagtcacat
     7261 ttatttggca gagacagctt taaatgtaaa ggatttggcc agtcaaacag ccttttatca
     7321 gcaagttatt ggtttagaaa tcttatctca aactgagaca gagtccattc taggccttgg
     7381 tggaaaagtc ttggtacagc tgattcaagc acaagagagc ggagaagtga gggaatatta
     7441 tggtctttac catttggcca ttctcttgcc cacacgcaag gctttagctg atgtcttgaa
     7501 acacctgaca gatttacaaa ttcctcttgt cggcggtgca gaccatggtt acagtgaagc
     7561 tatttacttg gaagatttgg agggaaatgg cattgaactc tatcgagata agccagtttc
     7621 cacatgggat attcgagaag atggacgcat tatcggagtg acggaggctc ttgcggcgca
     7681 ggacatttat gagttggggg aaagagtaga gccctttatc ctggcagaag gtacaagaat
     7741 ggggcatatt catctttccg tcaaggatag tcgaaagtcc agccagtttt atcaaaaggt
     7801 gttagggcta gagaataaat tcagtgtgcc tggcgctagt tggattgcgg ctggagatta
     7861 ccatcatcat ttagcagtta acgaatgggg aggaaaaggt ctggcttcgc gtaagcaagg
     7921 cttaccaggt ttagcctact atgtcatcga agtcgcacat aaaaaagaac tgttaacgat
     7981 tgcccaacga gcacaagaag ttgacgcacc aatcaaatgg atgacatcga tccaattgga
     8041 aatcacagac tcagatggca tcgtgacccg tattcgttta gcaaggtaaa attaggaatt
     8101 tttaataagt tataagtatt gctagtttgc tttttgaacg gtctttggta acagaactgc
     8161 gtgagaattt tgacaaagaa gctgattaga gatataatga agaaaaatcg agcaaggaaa
     8221 aagaactgac aaattcaaag tatattactt gtttgaaacg ttcagagggc cagttgtgtg
     8281 ggattcaaaa gatgattgaa ggagatcgtg actgtgctga tattgtgacg caactgacag
     8341 cagtgaaatc tagtgtagag tgcgtgattg agatgataat taccgaaaat cttactgaat
     8401 gtatcaatca gcccctagat gattctgaag ctcaaaagga acgcctagaa aaggctatcc
     8461 gatacttgat taaacggaaa taaatgaatg ttttgagacg atatgtgcaa aggtctatta
     8521 actattagaa tagtttaatg tgagtcaaaa gaagttgtaa aaaatctcta aaattaaaaa
     8581 aacgcatagt atcaggtgtt gaatgtactg acccccaaaa gttagatttt ttctgtctaa
     8641 cttttggggt gcagttcata agaaccttgg taatatgcgt tttattatgt agagacttat
     8701 actcttcgaa aatctcttca aaccacgtca gcttcacctt gccgtagata tggttactga
     8761 cttcgtcagt tctatccaca acctcaaaac agtgttttga gctgacttcg tcagttccat
     8821 ccacaacctt aaaacagtgt tttgagcagc ctgcggctaa cttcctagtt tgcactttga
     8881 ttttcattga gtattacttc atcttttcct gaaattgagt ttttgcccaa ctcctcttga
     8941 ttattctact tgaccgggcc aagcattcat accaccttct acgttggtaa cggtgaggcc
     9001 ttgggagctg agaaactggc aggtagatgc agagcggact cctccttgac aaatgacata
     9061 gtattcatgg tcaggtttga gttctttgta gccttgctct aaggtactta atggaagatt
     9121 tttggcacct ggtgcatgtc ctgcttggaa ttcatgtgct tcacggacat cgataagttc
     9181 tagattttca ttttggtatt ttccataaaa gtcagccatg ctgatactag tttccatatt
     9241 ctactccttc tgggttaacc attttgtata gtgaataagc gccgtcaagg ttttgggcgg
     9301 taaatcctgc ttgcttgagg ataggctctg cgatatagct gcgcaaacca ctgtggcagc
     9361 tgacgatgta ggcttgtttt ttgtccagtt catcagtttc ctccttgaca ctttgtcagt
     9421 ttttgataca atagtacaaa attagaggag gcaggctatg attcagaaac atgcgattcc
     9481 tattttagag tttgatgaca atcctcaggc ggttatcatg cccaatcacg aggggctgga
     9541 cttgcagttg ccaaagaagt gtgtttatgc atttttaggt gaggagattg accgctatgc
     9601 aagggaagta ggggcggatt gtgtcggcga attcgtttct gctaccaaga cctatccagt
     9661 ctatgtcatc aactacaagg gtgaggaggt ctgtctggct caggctcctg ttggctccgc
     9721 tccagcagcc cagtttatgg attggttgat tggctatggt gtggagcaga ttatctctac
     9781 tgggacctgt ggtgtcctag ctgatataga ggaaaatgcc tttctagtcc ctgttcgcgc
     9841 tttgcgagat gagggagcca gttaccacta tgtggcacct tgtcgttata tggaaatgca
     9901 gccagaggct attgctgcta ttgaggaagt tttggaagac agagggattc cttatgaaga
     9961 agtcatgacc tggacgacag acggttttta ccgagaaacg gctgaaaagg tggcttatcg
    10021 taaggaagaa ggctgtgctg ttgtggagat ggagtgttct gctcttgcgg cagtagctca
    10081 attgcgtggg gttctctggg gtgaattgtt gttcacagca gattctctag cggacttgga
    10141 ccagtacgac agtcgtgact ggggctcgga agcttttaat aaggcgctag aactgagttt
    10201 agcaagtgtt caccaccttt agttgtactg gcaaaggatt tgttttatca taaaatgtct
    10261 agctcatact tttcaaaaat atgtttaaac gaggtcacct tcctcttgtc ctaggcatgt
    10321 tgaggttggg aaaaatcttt aaaatcagaa aaacgtatca tatcaggtga tgaaaatttt
    10381 gatactatgc gttttatgtt ggtaagattt agagtgagat gaaatgatac tcttcgaaaa
    10441 tctcttcaaa ccaggtcagc ttcaccttgc cgtatagatg ttactgactt cgtcagttct
    10501 atctgcaacc tcaaaacagt gttttgagct gacttcgtca gttctatcca caacctcaaa
    10561 acagtgtttt gagcaacctg tgactagctt tctaagcgat gccttggttc tcattgccta
    10621 taatcaaaaa gagaaatttt ctcctgaaaa gcatatagag tagctggcgt taaaagctcc
    10681 tgtcttgctt ttttgaccta tagtcacatc tatcaagtat tgttcttgcc taagctatca
    10741 ataaaaaggt ggcatttttt aggcttggtg ttagtgaatt ttgccttatc ctatctaagt
    10801 catttcgagc tttttatggt acaatggaaa catgttattc aaattatcta aggaaaaaat
    10861 agagctaggc ttatctcgtt tatcgccagc ccgtcgtatt tttttgagtt ttgccttggt
    10921 cattttacta ggctctcttc ttttgagctt gccctttgtc caagttgaaa gctcacgagc
    10981 gacttatttt gatcatcttt tcactgctgt ctctgcagtc tgtgtgacag gtctctcaac
    11041 ccttccagta gctcacacct ataatatatg gggccaaata atctgtttgc tcttgattca
    11101 gatcggtggt ctagggctca tgacctttat tggggttttc tatatccaga gcaagcaaaa
    11161 gcttagtctt cgtagccgtg caactattca ggatagtttt agttatggag aaactcgatc
    11221 tttgagaaag tttgtctatt ctatttttct cacgaccttt ttggttgaga gcttgggagc
    11281 tattttgctt agttttcgcc ttattcctca acttggctgg ggacgtggtc tttttagttc
    11341 catttttcta gcgatctcag ccttctgtaa tgccggtttt gataatttag ggagcaccag
    11401 tttatttgct tttcagaccg atttactggt caatctggtg attgcaggct tgattattac
    11461 aggcggcctt ggttttatgg tctggtttga tttggctggt catgtaggaa gaaagaaaaa
    11521 aggacgtctg cactttcata cgaagcttgt actattgttg actataggtt tgttgttatt
    11581 tggaacggca actactctct ttcttgagtg gaacaatgct ggaacgattg gcaatctccc
    11641 tgttgccgat aaggttttag ttagcttttt tcaaacagtg acgatgcgaa cagctggctt
    11701 ttctacgata gattatactc aggctcatcc tgtgactctt ttgatttata tcttacagat
    11761 gtttctaggt ggggcacctg gaggaacagc tgggggactc aagattacga cattttttgt
    11821 cctcttggtc tttgcacgaa gtgagcttct aggcttgcct catgccaatg ttgcgagacg
    11881 aacgattgcg ccgcgaacgg ttcaaaaatc ctttagtgtc tttattatct ttttgatgag
    11941 cttcttgata ggattgattc tgctagggat aacagccaaa ggcaatcctc cctttatcca
    12001 cctcatattt gaaaccattt cagctcttag tacagttggt gtaacggcaa atctgactcc
    12061 tgaccttggg aaattggctc tcagtgttat catgccactt atgtttatgg gacgaattgg
    12121 tcccttgacc ttgtttgtta gcttggcaga ttaccatcca gaaaagaaag atatgattca
    12181 ctatatgaaa gcagatatta gtattggtta agaaaggaaa gagcatgtca gatcgtacga
    12241 ttggaatttt gggcttggga atttttggga gcagtgtcct agctgcccta gccaagcagg
    12301 atatgaatat tatcgctatt gatgaccacg cagagcgcat caatcagttt gagccagttt
    12361 tggcgcgtgg agtgattggt gacatcacag atgaagaatt attgagatca gcagggattg
    12421 atacctgcga taccgttgta gtcgcgacag gtgaaaatct ggagtcgagt gtgcttgcgg
    12481 ttatgcactg taagagtttg ggggtaccga ctgttattgc taaggtcaaa agtcagaccg
    12541 ctaagaaagt gctagaaaag attggagctg actcggttat ctcgccagag tatgaaatgg
    12601 gacagtctct agcacagacc attcttttcc ataatagtgt tgatgtcttt cagttggata
    12661 aaaatgtgtc tatcgtggag atgaaaattc ctcagtcttg ggcaggtcaa agtctgagta
    12721 aattagacct ccgtggcaaa tacaatctga atattttggg tttccgagag caggaaaatt
    12781 ccccattgga tgttgaattt ggaccagatg acctcttgaa agcagatacc tatattttgg
    12841 cagtcatcaa caaccagtat ttggataccc tagtagcatt gaattcgtaa agagggatga
    12901 cccctctttt ttgatgccca agatggcaaa tagagacaga agccccttgt cttctagtaa
    12961 aagttcttca aaggctggac tttatggtaa aatagaagga agtgacaaga gagagtaata
    13021 ctcaatgaaa atcaaagatc aaactaggaa actagctgcg ggctgctcaa aacactgttt
    13081 tgaggttgtg gatagaactg acgaagtcag ctcaaaacac tgttttgaag ttgcagatag
    13141 aactgacgaa gtcagctcaa aacactgttt tgaggttgtg gatagaactg acgaagtcag
    13201 ctcaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagctcaa aacactgttt
    13261 tgaggttgca gatagaactg acgaagtcag taacatctat acggcaaggc gacgttgacg
    13321 cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag gagcagatta
    13381 tgaagttatt gtctatcgcc atttctagct ataatgcagc agcctatctt cattactgtg
    13441 tggagtcgct aatgattggt ggtgagcaag ttgagatttt gattatcaat gacgggtctc
    13501 aggatcagac tcaggaaatc gctgagtgtt tagctagcaa gtatcctaac atcgttagag
    13561 ccatctatca ggaaaataaa ggccatggcg gtgtggtcaa ttgcggtttg gcagaggctt
    13621 ctgggcgcta ttttaaagta gttgacagtg atgactgggt ggatcctcgt gcctacttga
    13681 aaattcttga aaccttgcag gaacttgaga gcaagggtca agaggtggat gcctttgtga
    13741 ccaattttgt ctatgaaaag gaaggtcagt ctcgtaagaa gagtatgagt tacgagtcag
    13801 tcttgcctgt tcggcagatt tttgactagg accaggtcgg aaatttctcc aaaggccagt
    13861 atatcatgat acactcgctg atttatcgga cagatttgtt gcgtgctagc cagttctaac
    13921 tgcctgaaca tactttttat gtcgatgatc tctttgtctt tacgcccctt cagcaggtca
    13981 agaccatgta ctatctgcct gtcgatttct atcgttattt gattgggcgt gaggaccagt
    14041 ctgtcaatga gcaagtgatg attaagcgca ttgaccagca actcaaggtc aatcgactct
    14101 tggtagacca gcttgatttg tcccaagtga gtcatcctaa aatgcgagaa tatctgctga
    14161 atcatattga aatcacgacg gtgatttcca gtaccctgct caaccgatct gaaacagcgg
    14221 agcatctggc aaaaaaacgc caattgtgga cctatattca gcagaaaaat ccagaagtct
    14281 ttcaggctat tcgtaagacc atgttgagcc gtttgaccaa acattctgtc ttgccagatc
    14341 gcaaactgtc caatgtcgtc tatcaaatca ccaaatctgt ttatggattt aattaatata
    14401 agtgttttat aagagggatt taagaaaaat tttaactttt tcttagtcct ttttaatttc
    14461 aggagattat actagagtca tcaaataaag aaagactcta aggagaatcc tatgaaattc
    14521 aatccaaatc aaagatatac tcgttggtct attcgccgtc tcagtgtcgg tgttgcctca
    14581 gttgttgtgg ctagtggctt ctttgtccta gttggtcagc caagttctgt acgtgccgat
    14641 gtggtcaatc cgaccccagg tcaagtctta cctgaagaga catcgggaac gaaagagggt
    14701 gacttatcag aaaaaccagg agacaccgtt ctcactcaag cgaaacctga gggcgttact
    14761 ggaaatacga attcacttcc gacacctaca gaaagaactg aagtgagcga ggaaacaaac
    14821 tcttctagtc tggatacact ttttgaaaaa gatgaagaag ctcaaaaaaa tccagagcta
    14881 acagatgtct taaaagaaac tgtagataca gctgatgtgg atgggacaca agcaagtcca
    14941 gcagaaacta ctcctgaaca agtaaaaggt ggagtgaaag aaaatacaaa agacagcatc
    15001 gatgttcctg ctgcttatct tgaaaaagct gaagggaaag gtcctttcac tgccggtgta
    15061 aaccaagtaa ttccttatga actattcgct ggtgatggta tgttaactcg tctattacta
    15121 aaagcttcgg ataatgctcc ttggtctgac aatggtactg ctaaaaatcc tgctttacct
    15181 cctcttgaag gattaacaaa agggaaatac ttctatgaag tagacttaaa tggcaatact
    15241 gttggtaaac aaggtcaagc tttaattgat caacttcgcg c
//
LOCUS       DAVKJU010000051        14687 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVKJU010000051 DAVKJU010000000
VERSION     DAVKJU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.051"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            227..439
                     /locus_tag="VW191_001532"
     CDS             227..439
                     /locus_tag="VW191_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEU9624278.1"
                     /translation="MKFHYVNKWIYIILAIVLGWIGGHHFYAGYNRKGFLYLLFSFTF
                     IPMLLALFQVISALLKTPDSNGKILV"
     gene            459..689
                     /locus_tag="VW191_001533"
     CDS             459..689
                     /locus_tag="VW191_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624279.1"
                     /translation="MDYAQDYLDDILVRMAYHSSGIEGNTISLPETVSIILEKSIIVM
                     FNIANTSQRINVNAYTYFQNKNKKVQKKRQKS"
     gene            1581..2747
                     /locus_tag="VW191_001534"
     CDS             1581..2747
                     /locus_tag="VW191_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEU9624280.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKDGSTVYKTVEIKTYAELVENWLETYCHTVKKATLTIIKSRLNNYLL
                     PAFGEYKLDKLTPPVIQKQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVNKTLNCEKEVTTPKTKSSIRVIDLDNKTVLMLRLY
                     KNRQAQVGREIGLTYEKVFSNSFDEYRDARALRSRLEKHLKLSDCPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHSQISITMDTYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            2886..3458
                     /locus_tag="VW191_001535"
     CDS             2886..3458
                     /locus_tag="VW191_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624281.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(4503..6761)
                     /gene="glgP"
                     /locus_tag="VW191_001536"
     CDS             complement(4503..6761)
                     /gene="glgP"
                     /locus_tag="VW191_001536"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEU9624282.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(6787..8304)
                     /gene="malQ"
                     /locus_tag="VW191_001537"
     CDS             complement(6787..8304)
                     /gene="malQ"
                     /locus_tag="VW191_001537"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEU9624283.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEESLLDLTTIYRRINENLVDLKK"
     gene            8855..10126
                     /locus_tag="VW191_001538"
     CDS             8855..10126
                     /locus_tag="VW191_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9624284.1"
                     /translation="MSSKFMKSAAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            10237..11529
                     /locus_tag="VW191_001539"
     CDS             10237..11529
                     /locus_tag="VW191_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9624285.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            11531..12373
                     /locus_tag="VW191_001540"
     CDS             11531..12373
                     /locus_tag="VW191_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9624286.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            12626..13426
                     /locus_tag="VW191_001541"
     CDS             12626..13426
                     /locus_tag="VW191_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEU9624287.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQTPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            13436..14422
                     /locus_tag="VW191_001542"
     CDS             13436..14422
                     /locus_tag="VW191_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9624288.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            14489..>14687
                     /locus_tag="VW191_001543"
     CDS             14489..>14687
                     /locus_tag="VW191_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624289.1"
                     /translation="MRFIVGRFTSFSTEIESFPKIFLYSMKIKVQTRKLAAGCSKHCF
                     EVVDRTDEVSSKHGFEVVDETD"
BASE COUNT         4594 a         3024 c         2695 g         4374 t
ORIGIN      
        1 attatctaaa caaacaagta taagaaaaat gcttaaaaat ttaggatatc ctgaactggg
       61 attaattgga aagcatggat ttgtaaaaaa taagagtaca tatattgccg actctggagt
      121 ttcaaactta aaaaatgacg ataaaaaaga taataaaaat aatattagca ataataacga
      181 aataccacaa acaaaaattt ttgatgccaa aaccaaagct aaaaatatta aatttcatta
      241 tgtcaataag tggatttaca ttatattagc tatcgtttta ggttggatag gtggtcatca
      301 tttttatgca ggatataata gaaaaggatt tttatatcta ctattcagtt tcacttttat
      361 tccaatgctt cttgcacttt tccaagtaat ttcagcattg ttaaaaactc ctgattccaa
      421 tgggaaaatt ttagtttaat tttacagaaa ggagaatcat ggactacgct caagactacc
      481 tggacgatat actggtacgc atggcctacc attctagcgg tattgaaggg aacactattt
      541 ccctacccga aaccgtcagc attatcttag aaaaatccat aatcgtaatg ttcaatatcg
      601 ccaatacttc ccagcgaata aatgtcaatg cctacactta ttttcaaaat aaaaataaaa
      661 aagtacaaaa aaagcgacaa aaaagttgat ttttaataaa aaaacgatag agttatatta
      721 gaaagtcgct tgtaattctg atatgtctgc gtatttttcg taagttgcct attgggtaac
      781 ctgctatcag ttaagggctt ttatatttta caaaaaaatc agtgtatcgg cttattatgt
      841 tggtacactt ttctttttac tacactagtt ttctacaaca aaaaaaaaga caagctagct
      901 gctgctgact tgtctaatat cgcctgaact gaaataacgg gaaagattca ggcggttaca
      961 tgactagttt accatattat ccagaaaaac aaaaaaggta agtcaatacc ctgtcatcag
     1021 gctataactc tctaatgaag tataatgcgg tataatttag acaattttca acactttggc
     1081 attttagaaa cgcttaaagc gctctaaaca atatcaaaaa aaataaaact cttgaaatat
     1141 tggggcttgt atgctagaat gaaagcataa agggaaaaac agtgcttcta gcatatgtaa
     1201 ctaaaaagcc ccaccgttac agcgatgggg ttttttgcta ccctcttaaa gggttaaatg
     1261 ctacttgcta cgtctcttta tccatagctt gaaaagctca gataagacgt taacaagtaa
     1321 cggagctaga aaaagggaaa acaactctag catatataac acctcccttc tctaaattga
     1381 gggggcgaaa acattatatc aaaaacaccc atcacaaaca agcccatata agtcccatat
     1441 ctgccttatt cccttttctg gtacaattta ccgtctgact aattaaaatc gaaaataggg
     1501 gtattctcgt agcccctcgc atggtataaa ctcaaaatct tttctaattg cttgcctgct
     1561 gatggaagag gagtaaaacc atgaaaataa cagaatacac aaaaaaagac gggtctacag
     1621 tctatcgctc tagcgtctat cttggtatcg ataccgtaac aggtaagaaa gtcaagacga
     1681 ctatatcagg gcgaaccaag agagaactca aagccaaagc gctacaggct caaatagact
     1741 ttgaaaaaga cgggtctaca gtatataaga cggtggagat aaaaacttat gccgaactgg
     1801 tggagaattg gctggaaaca tactgccata cagtcaaaaa ggcaacactt acaataatca
     1861 agagtagact aaataactat ctacttccag cctttggaga atacaaacta gataagctaa
     1921 cacccccagt tattcaaaag caagttaatc aatgggcaaa agagtacaat caactaggca
     1981 aaggttacca agagtatccg caacttaact cgctaaataa acgaatactt aaatatgccg
     2041 tatccttgca agttatcccc ttcaacccag cccgtgatat tatcgtgcca cgtcgtaagg
     2101 aaaaagaagg gcaaaaactg aaatatctgg atgatgataa cttaaaaaaa ttcttgactt
     2161 acctggagca gctgccaaac acttacaaaa atttctacga tacggtgcta tataagacgc
     2221 ttttagcgac tggtttgcgt attcgtgagt gtttagccct caaatggtct gatattgact
     2281 tacaaaacgg tacactagac gtaaataaga ctctaaactg tgaaaaagaa gtcactaccc
     2341 ctaaaactaa atcaagtatt agggtaattg acttggataa taagacggtg ctcatgctgc
     2401 gactttacaa aaatagacaa gcgcaagttg gtagagaaat aggcttgact tatgaaaaag
     2461 tattttctaa ttcctttgac gaatatagag acgctagggc gcttaggtca agacttgaga
     2521 aacatttaaa actttctgac tgccctcgtt taacgttcca cgctttccgc cacactcacg
     2581 ctagtatctt gctcaatgcc ggactgcctt acaaagaaat acaaacaagg ctagggcatt
     2641 ctcaaatatc aataaccatg gatacatata gccacttgtc caaagacaac aagaaaaacg
     2701 ctacttcatt ttatgaaaaa gccattgaaa aattaaaaag ttcctaaaaa gtccacaaat
     2761 tttataaatc ggttatgaat caagccacaa ataccgctat actagggaat ttacccccta
     2821 tacttgaaat aaagcgcatt tcaaaataaa atgtaagcag atacaaataa aaaggagaaa
     2881 agctcatgaa caaattaatg aaatttattt cggttttttt gacgtcaatt gtgttaattg
     2941 tatcagcgat tccaagtgtt tcagctgtat acgcttctga acaagtatcg caaattgaaa
     3001 caaatatgga acttcaacct gtcacttctc taacagaaga acaaatcaat acacttgcaa
     3061 acgaaatcca atcttttcat ccagacgtct cacaacaatg gatcaaagaa gtaattaacc
     3121 gacaattaca aggcgattat acaatcccac ctacatactc tccatttaga gcagcttggc
     3181 aaggtattac agttaatcaa atgggtgctc tattagatac tgcaatagct ttagcattag
     3241 gaggaactac tgcaggcctt gcaaatctaa ttaaagtaaa aggaaaacat gcagcaaaaa
     3301 gtgctattcg ttcagcaatt tctagatatc taggtagttg gtttgtaaat gatgttgctt
     3361 tagaatccgc tatgaattta ttatcaccgg ggacttattt agcacaatta tgggataaaa
     3421 atgatgccat tcctaacaac ggaaggatta acttttaatc atgagatata caataaaatt
     3481 aattgggtat aacgtatttt cgttgtttat attctttatc ttatcaatgt tgtttaaagt
     3541 tccctttagg tggtggatca gcattgcaat gacgacttcg tcgtacattg tatataatta
     3601 cttaaagaaa aagagagaaa aggaatgatc ttataccaga tcattccttt tctgctttct
     3661 gtaaatccct cggagtgtgt ttggcgcttt gatacgaagt gtcaaagctg tataaacgtt
     3721 actatttcca aaaaagtaac agaaaattcc tgctttgtag gactgctttg ggtgagtaat
     3781 agacatgtcg actcacccta tctcctgatg gcgaaaagaa tttagcaatg gtgcaagttg
     3841 ttctgaactc gagaaaaaac atgccgaaat cgaagaaaag cgtgcagaac ttgaagcgaa
     3901 gaagcaagaa atgttgaatc gcttggataa atagaattta gaaaacagaa aaaactaaaa
     3961 agaatgtagg gtttatttcc ctacattctt aaaataactt agtacataaa tgacaatgct
     4021 gccactgccg tcaatattgc aaaaactatt ttccaaattt ttttattttt tccaaaaatc
     4081 ttatcaggta acaagcttat tacaagaaag gtaataaaca acaccaattt aagtgtgtat
     4141 atcataaaaa caccttcttt cctacaatta tattataata ctttatgtaa gcgaacacaa
     4201 gagatgtgag gtaaccaact acgaaaaaat ataaggctga agacagtaaa aaccagcatt
     4261 taaagccttc actgtatcta ggatttccct atttttagac aagattgacc caaatccaca
     4321 gcatttaaaa aagcgaattt caatcgaaac tcgctttttt acaagatgta ttagtataaa
     4381 tttgtatctt atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct
     4441 caaagcaccg ctttgaggtt gcagataaag ctgacgtggt ttgaagagat tttcgaagag
     4501 tattagttca agtgccagat gtcttcgtta tactgagcga ttgtacggtc agatgagaag
     4561 aatcctgctt tagaaatgtt aacgatgact ttatccaacc atgcgtcacg gtcttcgtag
     4621 tcagcaagca tttgctcttt gactttgatg tagtcttcca aatcaagaag agtcatgaac
     4681 cagtctttgt tgatcaattc attgtaaaga cgttccaagc gctctttgtt tccagctgca
     4741 agaactgcat cactaacgat gaagtcaacc aatggtttga tagcttcacg agcgtagaat
     4801 tcgcttgatt tgtaagctgc ttttgcgtaa aggtcgataa cagtttctga atcttcaccg
     4861 aagatgtaga tgttttcttc tccaaccaac tcagcgattt ccacgttagc accgtccata
     4921 gtaccaagtg tcaaagctcc gttcaacatg aatttcatgt taccagtacc tgaagcttct
     4981 ttagaagcaa gtgagatttg ttctgagata tcacatgctg ggataaggaa gcttgctgca
     5041 gtaacgttgt agttttcaac cataactact tgcaagtgtg gagctactgc tggatcgtta
     5101 gcaataactt ctgacatgca aaggatcaag tgaatgatat cttgagcgat tgtgtaggct
     5161 ggagctgctt taccaccaaa gaagattgtg attggacgag cagggatgtt accagctttg
     5221 atgtcaaggt atttgtggat tacgtacaaa gcgttcattt gttggcgttt gtactcgtga
     5281 agacgtttga tttggatatc aaagatagaa tttgaattga tttccacacc ttggtgttct
     5341 ttcaagtgac gagccaattt acgtttgttg tgagccttga tgctttccaa tttttctttg
     5401 acagctgctt tgtcttcata agacaaaagt ttttcaagct catctgcttc atggtgccaa
     5461 ccatctccaa gaatctcatc caagtagtga gacaatcttg ggttagcatg cataagccaa
     5521 cgacggaaag tgataccgtt tgttttgttg ttgaactttt ctgggtaaag gtcgtagaag
     5581 gctttcaact cagaattttt caagatttca gtatggagtg ctgcaacccc gttaacactg
     5641 tatccgtagt ggatatccat gtgagccatg tgaacacgtc cgctctcatc gatgatttga
     5701 acagctggat ctttgtactc tgccttcaca cgacggtcca attcttcgat gattggtacc
     5761 aagtgaggaa ccacttcttg caagaattca agaggccatt tttcaagcgc ctcagcaagg
     5821 attgtgtggt tagtgtaggc agtcatgcta cgaacaattg agattgcttc gtcaagctcg
     5881 ataccacgtg cagtcaaaag acgaatcaat tcaggaatca ccattgatgg gtgagtatcg
     5941 ttgatttgga caactgcgta gtcagcaagg tcatgcaagt tgcttccttt ttcgattgct
     6001 tcgtcgatga tcaattgcgc accgtttgaa accatgaagt attgttggaa gatacggagc
     6061 aattcacctt gacggtcact atcatctggg taaaggaaga gagttaagtt gcgagcgata
     6121 tctgtcttgt caaagttgat accatcttta ataatagaag aatcaactga atccaagtca
     6181 aacaaacgca agcggttttt agtcgctgtt tcataaccag taacatcaat atcgtaaaga
     6241 gttgatgtca aagtaaagtc tgcaaatggt acttggtagc tacggcttga gcgaaccaac
     6301 cagttttgct ctgtcaacca tgcatttgga attgtttctt gttggttgtt tttaagaact
     6361 tgttggaaaa gaccaaagtg gtagttaaga ccaacaccgt caccattcaa accaagagta
     6421 gcaattgagt cgataaagca ggcagccaaa cgtcccaaac caccattacc aagagatggt
     6481 tccaattcaa cttcttcaac ttcgatcaag tctttacctg cagctgcaag ttctttttta
     6541 acatcgtcgt aaagaccaag gttaatcaag ttgtttgaca agagtttacc aatcaagaac
     6601 tcagctgaga tgtagtaaac ttttttctta cccgtgttga ctggtttttg gctgcttgca
     6661 agcttgctgt agttaagaag agcaaggtaa agctcttcat tgctacattc tgcaatggtt
     6721 ttattgtaac gattttgtac aaattcttgt agtgataaca tgtttagttg tctcctgatt
     6781 attgtcttat ttctttaaat ctaccaaatt ttcattaatt cggcgataaa ttgttgtcaa
     6841 gtcaagcaaa ctttcctcga cagctggtgt caattgatct tcagtcatac gccaagacca
     6901 gtttccacca agggtagatg ggaagttcat acgagctgcc tcatccaatt ctagtaaatc
     6961 ttgcatagtt gcaattgcca taaagctaac tgatgaaaat actgtacgaa gcatagcgtg
     7021 taccactgtt tcgtattctt tacggttcgt gtaacgagcc atgtactcac gagtcgcatc
     7081 atcaatctca ttacggtacc aaccaagaac cgtattgtta tcgtgtgttc ctgtgtacat
     7141 aactgagtta gcaggtgcca agtgtgggct atcaatactt tcgtcttctg ggttgaaggc
     7201 aaattgaaga atcttcattc ctgggaagcc agtacgttca cgcaattcga tcacttcatc
     7261 tgtcatgaag ccaaggtctt ctgcgatgat gtttagctca ccaagttctt ccttaacggc
     7321 tgcaaaaagc ttgtagcctg gacctttcac ccactcacca ggtgctgctg tatcggaacc
     7381 agcagggatt tcccaataag attcgaagcc acggaagtgg tcgatacgaa cgatatcgta
     7441 gattttgaag ctttcacgca agcgttcaat ccaccatttg tagccgtctt tgtccattgc
     7501 ttcccagtca tagattgggt taccccaaag ctgaccagtt acagaaaact catctggtgg
     7561 gcatcctgcg atacaagtag ccttaccatt gacatctgtt ttgaagagat gtggatttgc
     7621 ccacatatca cttgaatctt ccgctacgta gattggcatg tccccaacga tttcgatgtg
     7681 gttgtcgtta gcgtaagctt tcaatttcaa ccattgttgg aagaagaagt attgagtcac
     7741 acggtggtaa accaacttgt ctgccaattg ctcacgatag ctttcaagtg ctgaagcttt
     7801 acgagcacga gcatctgcat ctggccattc agtccaagca agattgtcaa aatgctcttt
     7861 gatagccata tactcagcaa agagctcaag ccatgattgg ttgtcttgag caaatttctc
     7921 aaaatcttta acatctccga cttcaaagaa acgtttcacc gctttttcta aaagaggacg
     7981 acgtgcatag tagattttag catagtcaac ttcagacgca tcgctaccaa agtcaactcc
     8041 ttcaaggtca cttgcttcca acaaaccttg ctccaccaag atatctaaat cgataaaatg
     8101 agtgtttcct gcgaaggctg agaaagattg gtaaggagaa tccccgtaac tagttgctcc
     8161 taatggaagg atttgccagt aacgttgttt tgtacggacc aagaaatcaa cgaagtcgta
     8221 agcactttga ccaaatgatc cgattccgta agctcctgga agagaagaga tgtgcatcaa
     8281 cacaccactt tgacgttttt tcataatagc acctcgtgtg ttaaaataat ggaacgttgc
     8341 gtattttttc agacgcaaac gtttgcgtta cttataagta tactcctttt caagcataat
     8401 tgcaagcgtt tttaaaaaac ttttttgatt atttttaagg agactaactc attttagcaa
     8461 agataactta aaactatcta atcctaaagt ttgactctca cgagacagtc tacaaagtac
     8521 aaaacctcct tagggagctt gaatttatat gcaacaaagc acaaacgtct atagaaaata
     8581 gatatagatt agaaacaggg aggtagcccc tcctgatttc cctctttaga cagattccat
     8641 atatttttat taaaaatact gtattcttat ctatttcata cttttcttct attaaaatgc
     8701 aaaattatgc tatcaaatca agcttaaaaa ttttttaaaa tttttacaaa aaatacttgc
     8761 aaccgttttc tatttgtgct atactaagct cataaaggaa aacgtttgcg tttccttatc
     8821 aataaaactt gctattcttt aggaggaata cactatgtca tctaaattta tgaagagcgc
     8881 tgcggtgctt ggaactgcta cacttgctag cttgctcttg gtagcttgcg gcagcaaaac
     8941 tgctgataag cctgctgatt ctggttcatc tgaagtcaaa gaactcactg tatatgtaga
     9001 cgagggatat aagagctata ttgaagaggt tgctaaagct tatgaaaaag aagctggagt
     9061 aaaagtcact cttaaaactg gtgatgctct aggaggtctt gataaacttt ctcttgacaa
     9121 ccaatctggt aatgtccctg atgttatgat ggctccatac gaccgtgtag gtagccttgg
     9181 ttctgacgga caactttcag aagtgaaatt gagcgatggt gctaaaacag acgacacaac
     9241 taaatctctt gtaacagctg ctaatggtaa agtttacggt gctcctgccg ttatcgagtc
     9301 acttgttatg tactacaaca aagacttggt gaaagatgct ccaaaaacat ttgctgactt
     9361 ggaaaacctt gctaaagata gcaaatacgc attcgctggt gaagatggta aaactactgc
     9421 cttcctagct gactggacaa acttctacta tacatatgga cttcttgctg gtaacggtgc
     9481 ttacgtcttt ggccaaaacg gtaaagacgc taaagacatc ggtcttgcaa acgacggttc
     9541 tatcgtaggt atcaactacg ctaaatcttg gtacgaaaaa tggcctaaag gtatgcaaga
     9601 tacagaaggt gctggaaact taatccaaac tcaattccaa gaaggtaaaa cagctgctat
     9661 catcgacgga ccttggaaag cccaagcctt taaagatgct aaagtaaact acggagttgc
     9721 aactatccca actcttccaa atggaaaaga atatgctgca ttcggtggtg gtaaagcttg
     9781 ggtcattcct caagccgtta agaaccttga agcttctcaa aaatttgtag acttccttgt
     9841 tgcaactgaa caacaaaaag tattatatga taagactaac gaaatcccag ctaatactga
     9901 ggctcgttca tacgctgaag gtaaaaacga tgagttgaca acagctgtta tcaaacagtt
     9961 caagaacact caaccactgc caaacatctc tcaaatgtct gcagtttggg atccagcgaa
    10021 aaatatgctc tttgatgctg taagtggtca aaaagatgct aaaacagctg ctaacgatgc
    10081 tgtaacattg atcaaagaaa caatcaaaca aaaatttggt gaataaaaaa tttgttcaag
    10141 gggggtggaa atcaaatccc cctttgaatt tatcaataga gacacaaata atttagcttt
    10201 cttataaaaa agtagtatcc tatgaaagga gttaatatgg aaaagcaaca acctagtaaa
    10261 gcagccctgc tgtctatcat tcctgggtta ggacagattt acaataaaca aaaagccaaa
    10321 ggttttatct tccttggtgt aaccatcgta tttgtccttt acttcctagc acttgcaacc
    10381 cctgaattga gcaacctcat cactcttggt gacaaaccag gtcgtgataa ttccctcttt
    10441 atgctgattc gtggtgcctt ccatctaatc tttgtaatcg tttatgtact cttttatttc
    10501 tcaaatatca aagatgcaca tacgattgca aaacgcatta acaatggaat tccagttcca
    10561 cgcacactca aagacatgat caaagggatt tatgaaaatg gcttccctta cctcttgatc
    10621 attccatctt atgttgccat gaccttcgcg attatcttcc cagttatcgt aaccttgatg
    10681 atcgccttta ccaactacga cttccaacac ttgccaccaa acaagttgtt ggactgggtt
    10741 ggtttgacca actttacaaa catttggagc ttgagtacct tccgttctgc ctttggttct
    10801 gttctttctt ggactatcat ttgggctttg gcagcttcta ctttacaaat cgtaattggt
    10861 atcttcacag ctatcattgc caaccaacca tttatcaaag gaaaacgtat ctttggtgtt
    10921 attttccttc ttccttgggc tgtcccagcc ttcatcacta tcttgacatt ctcaaacatg
    10981 tttaacgata gtgtcggtgc tatcaacact caagtattgc caatcttggc taaattcctt
    11041 cctttccttg atggagctct tattccttgg aaaacagacc caacttggac taagattgcc
    11101 ttgattatga tgcaaggttg gctcggattc ccatacatct acgttttgac tttgggtatc
    11161 ttgcaatcta ttcctaacga cctttacgaa gcagcttata ttgacggtgc caacgcttgg
    11221 caaaaattcc gcaacatcac tttcccaatg attttggctg ttgcggcacc tactttgatt
    11281 agccaataca ccttcaactt taacaacttc tctatcatgt acctcttcaa tggtggagga
    11341 cctggtagtg tcggaggtgg agctggttca accgatatct tgatctcatg gatctaccgt
    11401 ttgacaacag gtacatctcc tcaatactca atggcggcag ctgttacctt gattatctct
    11461 atcattgtca tctcaatctc tatgatcgca ttcaagaaac tacacgcatt tgatatggag
    11521 gacgtctaag atgaataact caattaaact caaacgtaga ctgactcaaa gccttactta
    11581 cctttacctg attggtctat caattgtaat tatctatcca ctgttgatta ccattatgtc
    11641 agcctttaaa gcaggtaacg tctcagcctt taaactagat actaatatcg acctcaattt
    11701 tgataacttt aaaggcctct tcactgaaac cttgtacggt acttggtacc tcaacacttt
    11761 gattatcgcc ttaattacca tggctgttca aacaagtatc atcgtacttg ccggttatgc
    11821 ttacagccgt tacaacttct tggctcgtaa acaaagtttg gtcttcttct tgatcatcca
    11881 aatggtgcca actatggccg ctttgacagc cttcttcgtt atggcgctta tgttgaacgc
    11941 ccttaaccac aactggttcc tcatcttcct ctacgttggt ggtggtatcc cgatgaatgc
    12001 ttggctcatg aaaggctact tcgatacagt gccaatgtct ttagacgaat ctgcaaaact
    12061 agacggtgca ggacacttcc gccgcttctg gcaaattgtt ctaccacttg ttcgcccaat
    12121 ggttgccgta caagctctct gggccttcat gggacctttc ggggactaca tcctctctag
    12181 tttcttgctt cgtgagaaag aatactttac tgttgccgta ggtctccaaa ccttcgttaa
    12241 caatgcgaaa aacttgaaga ttgcctactt ctcagcaggt gctatcctca tcgcccttcc
    12301 aatctgtatt ctcttcttct tcctacaaaa gaactttgtt tcaggactta caagtggtgg
    12361 cgacaaggga taatttatcc ccgccaccct ttttcatttt atactcttcg aaaatctctt
    12421 caaaccacgt cagctttatc tccaacctca aagttgtgct ttgagcaacc tgtggctagt
    12481 ttgcactttg attttcattg attattagca attgtcactg taaataatat ccttgtagca
    12541 agcaattttt ctcctagact tgaaataaag cgcatttcac tatataataa tactcatata
    12601 gaaaacacct tttagaaaga tacctatgct tccatatcca ttttcctatt tttcaagtat
    12661 ttgggggttt cgtaagcccc tgtccaaacg tttcgagctc aactggtttc aacttctctt
    12721 taccagtatc ttccttatca gcttgtctat ggtacccatt gctatccaaa acagctccca
    12781 ggagacctat ccgctagaaa cttttatcga taatgtctat gaacctctga caaatgaggt
    12841 tgtccaggat ctctctgaac atgctacaat tgtcgatggc acattaactt atactggaac
    12901 agctagtcaa accccttctg ttgtgattgg tccaagtcaa atcaaggaat tacctaagga
    12961 cttgcaactg catttcgata caaatgagct agtcatcagc aaggaaagca aggagctgac
    13021 ccgcatctct taccgagcca ttcagactga gagtttcaaa agcaaagaca gcttgaccca
    13081 agcaatttct aaagactggt accaacaaaa tcgtgtctat atcagcctct tcctagttct
    13141 cggtgcgagc ttcctctttg gtttgaattt ctttatcgtc tctctaggag ctagtcttct
    13201 cctttatatc actaagaaat cacgcctctt ttcatttaga acctttaaag agtgctatca
    13261 ctttatcttg aactgtttag gattgccgac tctgattaca cttattttgg gattatttgg
    13321 ccaaaatatg acaaccctga ttactgtaca aaatattctt tttgttctgt atctggtcac
    13381 tatcttttat aaaacacatt tccgtgatcc aaattaccat aaataggaga tttttatgcc
    13441 cgttacgatt aaagacgtgg ccaaggctgc tggtgtttcg ccttcaaccg taacccgtgt
    13501 tattcaaaat aaatcaacca ttagcgacga aacaaaaaaa cgcgttcgca aagctatgaa
    13561 ggaactcaac taccacccaa acctcaacgc tcgtagcttg gtaagcagct atactcaggt
    13621 tatcggatta gttcttcctg atgactcaga cgccttctac cagaatcctt tctttccatc
    13681 ggttctacgt ggcatctctc aagtcgcatc tgaaaaccac tatgccattc agatagcaac
    13741 agggaaagat gagaaggagc gtctcaacgc tatttcacaa atggtctacg gcaagcgtgt
    13801 agatgggcta atctttctct atgcccaaga agaagaccct ctcgtaaaac tcgtcgcaga
    13861 agaacagttc cccttcctta tcctaggtaa atctctatct cctttcatcc cacttgtcga
    13921 caacgacaat gttcaagctg gttttgatgc gactgaatat ttcatcaaaa aaggctgcaa
    13981 acgcattgcc tttatcggag gaagtaaaaa gctcttcgtg accaaagacc gtttaacagg
    14041 ctatgaacag gcgcttaaac attacaaact taccactgac aacaatcgca tctactttgc
    14101 cgacgagttt ctggaagaaa agggctataa atttagcaag cgattattca agcacgatcc
    14161 acaaattgat gctatcatca caaccgatag cctcctagct gaaggtgttt gtaactatat
    14221 tgccaaacac cagctggatg tccctgttct cagctttgac tcggttaatc ccaagctcaa
    14281 cttggcagcc tatgtcgata tcaatagttt agagcttggt cgtgtttccc ttgaaactat
    14341 tctccagatt attaatgata ataaaaacaa taaacaaatt tgttaccgtc aattgatcgc
    14401 ccacaaaatt atcgaaaaat aagagactgg gcaaagagtc gttaaaagca aaaacgcata
    14461 ctatcaggta ttgaaaaaac ttgatactat gcgttttatt gtgggaagat ttacttcctt
    14521 ttctactgaa attgagtctt ttcccaagat ctttttatac tcaatgaaaa tcaaagtgca
    14581 aactaggaag ctagccgcag gttgctcaaa acactgtttt gaggttgtgg atagaactga
    14641 cgaagtcagc tcaaaacatg gttttgaggt tgtagatgaa actgacg
//
LOCUS       DAVKJU010000052        14510 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVKJU010000052 DAVKJU010000000
VERSION     DAVKJU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.052"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            688..1964
                     /gene="ltrA"
                     /locus_tag="VW191_001544"
                     /pseudo
     CDS             688..1964
                     /gene="ltrA"
                     /locus_tag="VW191_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2420..3598
                     /locus_tag="VW191_001545"
     CDS             2420..3598
                     /locus_tag="VW191_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9624290.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTVYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3912..4043
                     /locus_tag="VW191_001546"
     CDS             3912..4043
                     /locus_tag="VW191_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEU9624291.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4064..5614
                     /gene="dltA"
                     /locus_tag="VW191_001547"
     CDS             4064..5614
                     /gene="dltA"
                     /locus_tag="VW191_001547"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEU9624292.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     ITDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDSVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5611..6855
                     /gene="dltB"
                     /locus_tag="VW191_001548"
     CDS             5611..6855
                     /gene="dltB"
                     /locus_tag="VW191_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEU9624293.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6869..7108
                     /gene="dltC"
                     /locus_tag="VW191_001549"
     CDS             6869..7108
                     /gene="dltC"
                     /locus_tag="VW191_001549"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEU9624294.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7101..8369
                     /gene="dltD"
                     /locus_tag="VW191_001550"
     CDS             7101..8369
                     /gene="dltD"
                     /locus_tag="VW191_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEU9624295.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKGELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8524..8964
                     /gene="adcR"
                     /locus_tag="VW191_001551"
     CDS             8524..8964
                     /gene="adcR"
                     /locus_tag="VW191_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEU9624296.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8964..9668
                     /locus_tag="VW191_001552"
     CDS             8964..9668
                     /locus_tag="VW191_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9624297.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVSHA"
     gene            9661..10467
                     /locus_tag="VW191_001553"
     CDS             9661..10467
                     /locus_tag="VW191_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9624298.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10477..11982
                     /gene="adcA"
                     /locus_tag="VW191_001554"
     CDS             10477..11982
                     /gene="adcA"
                     /locus_tag="VW191_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEU9624299.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVTKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYVEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISIMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(13110..13883)
                     /locus_tag="VW191_001555"
     CDS             complement(13110..13883)
                     /locus_tag="VW191_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9624300.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCVYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            14059..>14510
                     /locus_tag="VW191_001556"
     CDS             14059..>14510
                     /locus_tag="VW191_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEU9624301.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTN"
BASE COUNT         4349 a         2653 c         3074 g         4434 t
ORIGIN      
        1 ctaccggtga acccaatagc gattcccaag cctgactatc gtgaggtagc ggattaaaac
       61 ggtctgggga tagaccgttt taagtctgac gcaggaaata agaatcgtca gaggaaggga
      121 tagcgaaatc gtggctctac gaacaggaac gtgatagtaa ggcgtatata gcggataagg
      181 aggcttcaaa ctctaaagtc caaaaaggta gtcgtaacct atatgtgtaa atcacgagag
      241 taattgaatt cggactaagg tttgtgtgaa aaagataaat ctttctagag tctaaagact
      301 ctgcgtcaga tttcctattt tcactgtaac cttttaacgt cctcatatct tgtataaacg
      361 aggaaagatg tacgacttat cccgtgaggt ttcatgagcg ctgaaagcgt agtaacaacg
      421 aatcatgaga agtcagccga gcccatagta gtgaggaaac ttctgtagtg gaagtggagc
      481 gaaggggtga atactcaaac agtctgggga gagactgttt gaggtctgtc gctagaaaga
      541 gaaaacgaca gatcgaagta atcctacttc acttgtgtct gtaaaatgag tggtctgata
      601 gaactggact ttgacacgta atgactagat gaaggttcac caatataaga tgtccctcag
      661 gcaccaaaac aagaaaggaa tacgcacatg tcaaaactgc tagataagat attatcacgc
      721 gaaaatatgc tggaagccta caatcaagta aaatccaata aaggctcagc tgggattgat
      781 ggaatgacta tcgaagagat ggataattat ctcagacaaa actggcgctt gactaaggaa
      841 ctgataaaac agagaaaata taagcctcaa ccagttctta aagttgagat acctaaacca
      901 gacggaggca tccgtcaact aggaatttca acagttatgg atagaatgat tcaacaggcc
      961 attgtccaag tcatgagccc catttgtgaa ccccatttct cagatacgag ttatggtttc
     1021 agaccaaata ggtcatgtga aaaagccatc atgaagctct tagaatactt aaatgacggc
     1081 tatgagtgga tagtggatat agacctagag aaatttttcg atacagttcc tcaagataga
     1141 ttgatgtcct tagtacataa cattatcgaa gacggagata cggaatcctt gattcgtaag
     1201 tatcttcatt caggtgttat cattaatggt caacgttata aaacactagt tggtacacca
     1261 cagggaggaa atttatctcc tctcttatcc aatatcatgc ttaatgaatt ggacaaggaa
     1321 ttagaaaaga ggggacttcg atttgtgcgc tacgcagatg attgtgtgat acggtcggaa
     1381 gcgaggcagc cgctaagcgt gtgatgtatt cagtcagtcg ttttattgag aaacggctag
     1441 gtttgaaagt aaacatgacc aagactaaga ttaccagacc aagagagttg aaatatctag
     1501 gttttgggtt ctggaaatca tcagatggtt ggaaaagtcg tccacatcaa gatagtgttc
     1561 ggagatttaa gcttaaattg aagaaactaa cacagaggaa atggagtata gacctaacaa
     1621 gacgtattga gcaactgaat ttgtctattc gaggatggat aaactatttc tcattgggaa
     1681 atatgaaaag tatagtcgcc agcatagatg agcgcttgcg tactcgccta cgagtgatta
     1741 tttggaagca atggaagaag aaatcgagac gattatgggg attgcttaag ttaggagttc
     1801 ctaaatggat agcagataag gtatctggct ggggcgacca ttatcaatta gtagctcaga
     1861 agtcggtact taaacgtgct atatcaaaac cagtcctgga aaaacgtgga ctggtttcgt
     1921 gtttggatta ttaccttgaa cgacatgcgt taaaagttag ttgaaccgcc gtatgccgaa
     1981 cggcacgtac ggtggtgtga gaggggctag agattatccc ctactcgatt aactcccctg
     2041 aaatttattt taattatgca aatttcacgt atttttgatg ctgagacgac gatcctggga
     2101 acttttcaga tatttttttg actatctaaa tctatcatta gaaaagctta gagcgccaaa
     2161 ggatttgagc gtttttctga tttttaagac tttttccagt ctctttttcg attgaagatg
     2221 taattattct actaactaac taacttctta gtactagcca acaacgataa tcataattcc
     2281 tcctaaaatt aggaataata aaggcaatag tttttgtttt ttcatgtaaa aaacctcact
     2341 tttgttttct gctattttat gctaaaatat taaaaatcaa atttaattcc aaagtttgta
     2401 actaaagggg gagcgctaca tgtctaattc atttgtcaag ttgttagtct ctcaattatt
     2461 tgcaaattta gcagatattt tctttagagt aacaatcatt gctaacatat acattatttc
     2521 aaaatcagta attgccacat cactagttcc tatcttaata ggaatatcct cttttgttgc
     2581 gagtctttta gttccgttgg ttactaaaag gttagcgcta aatagggttt tatctttatc
     2641 tcaatttgga aagactatat tattggcaat actggtagga atgtttaccg taatgcaatc
     2701 cgtagcgcct ttggtgacct atctatttgt tgttgcaatt tccatactag atggttttgc
     2761 agcacccgtt tcctatgcta ttgtgccacg ctatgcgacc gatttgggta aggctaattc
     2821 agccttatca atgactggtg aagctgttca attgataggt tggggattag gtggactctt
     2881 gtttgcaaca attggtctgt tacctaccac gtttatcatt ttagtcttgt atatcatttc
     2941 tagctttctg atgttatttc ttcctaacgc tgaagtggag gtgttagagt cagaaactaa
     3001 tcttgaaatt ttgctcaaag gttggaagtt agttgctaga aatcctagat taagactttt
     3061 tgtatcagca aatttattgg aaattttttc aaatacgatt tgggtttctt ccattatact
     3121 tgtttttgta acggagttat taaataaaac ggaaagttac tggggatatt ctaatacagt
     3181 atactctatt ggtattataa ttagtggctt aattgctttt aggctatctg aaaagttcct
     3241 tgctgctaaa tgggaaagta ttctttttcc tcttgtagct atggcaatag tgacactgac
     3301 tattctatat tttccaaacg cacagatgtt tttattattt tcagctttag ttggtatgtt
     3361 atcgcaacta aaagaagttc ctgaaagtgt atttcttcag gaaacagtag aggaaaatca
     3421 tttagttaat gtctattccg ttcttgaagt gatttctaca ttagcatttt cagtatttgt
     3481 tttgctaatg agctatatta ctgagagttt tggtattagc atcagttttt ggctatcagc
     3541 catttgtctg atgatagaag ctattttaat ttatatcaga cgagattatt ttaaatgaga
     3601 gagtggttaa aaagtcttta aaaatcagaa aagcgcatag tatcaggtgt tgaaaaaact
     3661 tgatattgtg tgttttatta tgggaagatt tacttcattg tctcctgaaa tggagttttg
     3721 aagcaggatc actctatttg aaaatataca tagaaaaacc gcataatcct taaaataagc
     3781 tgaaatctat tggaaacata cggtaaacaa ttgaaaagat actatataaa gtggtacaat
     3841 ggtagaaaaa taagctagta agaatcactc ttattttagc gaaaaattac agaaatgaat
     3901 ggtttttctt gatgaaacag agaaaagaat tgtacctctt tcttggtcgg acagccttgt
     3961 attttcttat ctttctaggg ctgctttact tctttagcta tcttggtcag ggtcaaggaa
     4021 gctttatcta taatgaattt taacttgaag gagaatccct attgtgtcaa ataaaccaat
     4081 agcagatatg attgaaacca ttgagcattt tgctcagaca cagcctagct atcctgtcta
     4141 taatgttttg gggcaggaac acacttatgg cgatttaaag gctgattcgg atagtttggc
     4201 tgcagtcatt gaccaactag gcttgcctga aaagtctcct gtggttgttt ttggtggcca
     4261 agaatatgaa atgttggcaa cctttgtagc gctgactaag tcaggtcatg cctacattcc
     4321 aattgatagc cattcggcct tggagcgagt ttcagctatt ttagaagtag cagagccaag
     4381 cttgattatt gccatttcag cctttccctt ggagcaggtt tctacaccaa tgataaatct
     4441 agctcaggtt caagaagcct ttgcccaagg gaacaactat gaaatcacgc atccagtcaa
     4501 gggagatgat aattactaca ttatctttac ttctggtacg actggtaagc ctaagggagt
     4561 gcagatttca catgataatc tcctcagctt tacaaactgg atgattacgg ataaggaatt
     4621 tgcgacaccg agtcgtccgc aaatgctggc acagccacct tattcttttg acttgtctgt
     4681 catgtattgg gcaccgacct tggcacttgg tggtacgctt ttcactcttc cttcagtcat
     4741 cactcaggac tttaagcaac tctttgcggc tatcttttca ttgccaatcg ctatctggac
     4801 atcaacacca tcctttgcag atatggccat gttgtctgaa tacttcaaca gtgaaaaaat
     4861 gcctggaatc acgcatttct actttgatgg tgaagaattg acggtcaaaa cagctcaaaa
     4921 actgcgcgag cgtttcccaa atgcccgtat catcaatgct tacggcccaa cagaagcgac
     4981 agtagctctg tcagcagttg ccgtgacaga cgagatgtta gcgactctca aacgcctacc
     5041 aatcggctat accaaggctg attctccaac ctttatcatt gacgaggaag gaaataaact
     5101 gccaaatggc gaacagggag aaatcattgt ttctgggcca gctgtttcaa aaggttatat
     5161 gaacaatcct gaaaaaacag cagaagcctt ctttgagttt gaaggtctgc cagcctatca
     5221 cacaggcgat gtgggaacca taacagatga gggcttgctt ctctacggcg gacgcatgga
     5281 cttccagatt aagttcaacg gttaccgcat tgagttagaa gatgtctctc aaaacctcaa
     5341 caagtctcgc tttatcgaat ctgctgtcgc agtaccgcgc tataacaagg accacaaggt
     5401 acaaaatcta ttggcttatg tcatcttaaa agacagtgtt cgtgagcagt ttgagcgaga
     5461 tatcgatatt accaaggcca tcaaggaaga cctgacagac atcatgatgt cctacatgat
     5521 gccatctaaa ttcctttacc gagacagttt gccactgact ccaaatggaa agattgacat
     5581 caaaggattg attaacgagg tgaataagag atgatggagt tttttcaaca gcttcctcat
     5641 ttagagccat atggcaatcc tcagtatttt gtttatgtga ttgctgcaac cttgcccatc
     5701 tttataggcc tctttttcaa gaaacgcttt gcctggtatg aagtgttggt aagtctcttc
     5761 tttattgtca ccatgttggt gggtggaaag accaatcaac tagctgcctt gggtatttac
     5821 ctttgctggg aaatattgct cctgcttttc tacaagcatt atcgaaaaag caaggatggc
     5881 aagtgggtct tctacttagt tagttttctg tccctacttc cgattatctt tgtcaaggtg
     5941 caaccagcta tcaatggaac gcagtctttg cttgggttct tgggaatttc ttacctgacc
     6001 tttcgttcgg ttgggattat catcgagctg agagatggag tgattaagga ttttaccctc
     6061 tgggaattcc tccgtttcct tctcttcatg ccaactttct cgagtggtcc aatcgatcgc
     6121 tttaagcgat ttaatgaaaa ttatcaggct attcctgagc gagatgagtt gatggatatg
     6181 ctggatgaat ctgtccgcta tatcatgtgg ggctttttgt ataagtttat cctagctcat
     6241 gttttaggag agaccttact acctcctctg aagaatttag ccttgcagtc aggtggcttc
     6301 tttaatctct atgccttggc agttatgtat acttttggtc tggagctctt ctttgacttt
     6361 gcaggttatt ctatgtttgc tttggccatc tcaaacttga tgggaatccg tagccctatc
     6421 aactttaaca agcccttttt atcaagggat ttaaaggagt tttggaatcg ctggcatatg
     6481 agtctgtcct tctggttccg tgactttgtc tttatgcgaa tggtgatggt gttaaccaga
     6541 aagaaagtct ttaaaaatcg taatgtaacc tcaagcatgg cctacattgt aaatatgctg
     6601 attatgggat tttggcatgg tgtgacctgg tactatatcg cctatggact ctttcatgga
     6661 ctaggcttgg tcatcaatga tgcctgggtt cgcaagaaaa aaacgctcaa taaggaacgg
     6721 aaaaaagcag ggaaggctgc cctacctgag aatcgctgga ttcagttgct tggcatggtt
     6781 gtcactttcc atgttgtcat gttgtcattc ttaatctttt ctggattctt gaataatcta
     6841 tggtttaaaa aataaaagga aataaaatat ggatatcaaa tcagaagtta ttgaaattat
     6901 tgatgagttg tttatggaag atgtttctga catgatggat gaagatcttt ttgatgcagg
     6961 tgtcttggat agtatgggaa cggttgagtt gattgtggag attgagaacc gttttgacat
     7021 tcgtgtccct gtaacagagt ttggtcgtga cgactggaat acagctaata aaatcatagc
     7081 tggtattgtg gagctacaaa atgcttaaac gcttatggat gatcttcgga ccggtcttga
     7141 tcgctggttt gttggttttt ctgctcattt tcttttatcc tactgagatg catcataatc
     7201 taggagctga aaagcgttca gcagtggcta ctactatcga tagttttaag gagcgaagtc
     7261 aaaaagtcag agcactatct gatccaaatg tgcgttttgt tcccttcttt ggctctagtg
     7321 aatggcttcg ttttgacggt gctcatcctg cggtattagc tgagaaatac aatcgttcct
     7381 accgtcctta tcttttagga caggggggag ctgcatcgct taaccaatat tttggaatgc
     7441 aacagatgtt accacagctg gagaataaac aagttgtgta tgttatctca cctcagtggt
     7501 tcagtaaaaa tggctatgat ccagcagcct tccagcagta ttttaatgga gaccagttga
     7561 ctagttttct gaaacatcaa tctggggatc aggctagtca atatgcagcg actcgcttac
     7621 tgcaacagtt cccaaacgta gctatgaagg acctggttca gaagttggca agtaaaggag
     7681 aattgtcgac agcagacaat gaaatgattg aattattggc tcgttttaat gaacgccaag
     7741 cctccttttt tggtcagttt tcggttagag gctatgttaa ctacgataag catgtagcta
     7801 agtatttaaa aatcttgcca gaccagtttt cttatcaagc aatagaagat gttgtcaaag
     7861 cagatgctga aaaaaatact tccaataatg agatgggaat ggaaaattat ttctataatg
     7921 agcagatcaa gaaggatttg aagaaattaa aggattctca gaaaagcttt acctatctca
     7981 agtcgccaga gtataatgac ttgcagttgg ttttaacaca gttttctaaa tctaaggtaa
     8041 acccgatttt tatcattcca cctgttaata aaaaatggat ggactatgct ggtctacgag
     8101 aggatatgta ccaacaaacg gtgcagaaga ttcgctacca gttagaaagt caaggtttta
     8161 ccaatatagc agatttttct aaggacggtg gggagccttt ctttatgaag gacaccattc
     8221 accttggttg gttgggttgg ttggcttttg acaaggcagt tgatcctttc ctatccaatc
     8281 ccacaccagc tccgacttac catctgaatg agcgcttttt cagcaaagat tgggcgactt
     8341 atgatggaga tgtcaaagaa tttcaataga taatataata tagaagagtt caagaatgaa
     8401 actagttttc acgttttttc ttgactcttt ttttgcttgt gatataatta actggtaaac
     8461 aaaatgtcaa agaatagtat ttttctctta aaaaagagaa gtctaaaagg aaaggagtca
     8521 gatatgagac agctagcaaa ggatatcaat gcttttttga atgaggtgat tttgcaggcg
     8581 gaaaatcagc atgaaatcct aataggtcat tgcactagcg aggtggccct gaccaatact
     8641 caggagcata tccttatgct cttgtcagag gaatctttaa caaattcaga attggcccgt
     8701 cgtctcaatg tcagtcaggc ggcagttacc aaggccatta agtctttggt caaggaaggg
     8761 atgttggaaa catctaaaga ttctaaagat gcgcgtgtga ttttttatca gttgactgac
     8821 ttggctcgtc caatcgctga ggagcaccac catcaccatg agcatacact tttaacctat
     8881 gaacaagtgg cgactcagtt tactccaaat gaacaaaaag tgattcagcg gtttttgact
     8941 gctttagtag gagaaatcaa ataatgagat atattacggt agaggatttg tccttctatt
     9001 atgataagga gcctgttctt gaacatatca attatagtgt tgatagtggg gaatttgtta
     9061 ccttgactgg ggaaaatgga gcggctaaga cgacgctcat caaggccagt cttggaattc
     9121 tgcaaccacg cattggaaag gtggctattt caaagacaaa tacgcaaggt aagaaattga
     9181 gaatagccta tcttcctcaa caaattgcca gttttaatgc tggttttcca agtacggtct
     9241 atgaatttgt caagtcgggt cgctatccga gaaaaggctg gttccgtcgt ttgaatgctc
     9301 atgatgagga gcatatcaag gccagtctgg actcagttgg catgtgggaa catcgagaca
     9361 aacgcttggg gtctctatct gggggacaaa agcagcgagc ggtaattgcg cgtatgtttg
     9421 cttctgaccc tgatgtgttt atcctagacg agccgacaac ggggatggat gcaggaagta
     9481 aaaatgaatt ttacgaactc atgcaccaca gcgcccatca tcatggcaag gctgttttga
     9541 tgattaccca tgaccctgaa gaagttaagg attatgcgga tcgcaatatt catctagtcc
     9601 gtaaccaaga ctcgccatgg cgttgtttca acgttcatga gaatggccag gaggtgagcc
     9661 atgcttagtt tattatctta cgactttata caacgcgcct ttttggcggt tattgctatg
     9721 agtcttttct caccggtatt gggaaccttc ctcatcttgc gtcgtcagag tttgatgagt
     9781 gataccctta gccacgtctc actttcaggt gtagcctttg gtctggtttt ggggatttct
     9841 ccaactgttt ctactattgc cattgtcttg attgcggcgg tctttctgga gtatctccgt
     9901 acggtttaca agagctttat ggaaatcggg acagctatcc tcatgtcaac aggtctggct
     9961 gtttctctga ttgtcatgag caagggtaaa agctcgagtt caatgagttt ggaccaatat
    10021 ctctttggtt cgatcgtgac tatcagtgaa gaacaggtca tttccctctt tgtcattgcg
    10081 gcggttgttt tgattttgac ctttctcttt cttcgtccta tgtatatctt aacttttgat
    10141 gaagatacgg cctttgtgga tggcttgcca gttcgtacca tgtccattct ttttaacatg
    10201 gtgacagggg tggctattgc ccttatgatt cctgcagcag gagctcttct ggtatcgacc
    10261 attatggtct tgccagctag tattgccctg cgtctgggga aaaactttaa atcggttatg
    10321 ctgcttgcca gtgcgattgg ctttttggga atggtagcag gactttacat ttcctactat
    10381 gcagaaacac ctgcaagtgc aagtattacc attatttttg taactgtctt tatactaatc
    10441 agtttagtaa gacgttttat caaataggag acgaacgtga aaaaaattag cttattacta
    10501 gccagtctat gtgccttgtt tttagtggct tgttccaatc aaaaacaggc agatggtaaa
    10561 ctcaatatcg tgacaacctt ttaccctgtc tatgaattta ccaagcaagt cgcaggagat
    10621 acggctaatg tagaactcct aatcggtgct gggacagaac ctcatgaata cgaaccatct
    10681 gccaaggcag ttaccaaaat ccaagatgca gataccttcg tttatgaaaa tgaaaacatg
    10741 gaaacatggg tacctaaatt gctagatacc ttggataaga aaaaagtgaa aaccatcaag
    10801 gcgacaggcg atatgttgct cttgccaggt ggcgaggaag aagaggggga ccatgaccat
    10861 ggagaagaag gtcatcacca tgagtttgac ccccatgttt ggttatcacc agttcgtgcc
    10921 attaaactag tagagcacat ccgcgacagc ttgtcagcag attatcctga taaaaaagag
    10981 acctttgaga agaatgcagc tgcctatatc gaaaaattgc aagccttgga taaggcttac
    11041 gtagaaggct tgtctcaagc aaaacaaaag agctttgtga ctcaacacgc agcctttaac
    11101 tatcttgcct tggactatgg actcaaacaa gtcgcaatct caggactttc tccagatgca
    11161 gagccatcag ctgctcgctt ggcagaattg acagagtatg tcaagaaaaa taaaatcgcc
    11221 tatatctatt ttgaagaaaa tgcctcacaa gcccttgcta acacactttc aaaagaagca
    11281 ggtgtcaaaa ctgatgtcct caatccttta gaaagtctga cagaagagga caccaaggct
    11341 ggagaaaatt acatctctat catggagaaa aacctcaagg ctttgaaaca aacaacagac
    11401 caagaaggcc cagcaatcga gcctgaaaag gcagaggata ccaagacagt ccaaaatggt
    11461 tacttcgagg atgcagctgt caaggaccgc accttgagtg actatgcagg taactggcaa
    11521 tcagtttatc ctttccttga agacggcacg tttgaccaag tctttgacta caaggctaag
    11581 ttgactggta agatgaccca ggctgagtac aaggcttact atacaaaagg ctatcagaca
    11641 gatgtgacta agattaacat tactgataat actatggaat ttgttcaagg tggacaaagc
    11701 aagaaataca cttacaagta tgtcggtaag aaaattttga cttacaagaa aggcaatcgt
    11761 ggcgtgcgtt tcctctttga agccacagat gctgacgctg gacaattcaa gtatgttcag
    11821 tttagtgacc acaatatcgc tccagttaag gcagaacatt tccatatctt ctttggaggc
    11881 acaagccaag aagccctctt tgaagaaatg gacaactggc caacctacta cccagataac
    11941 ctatctggcc aagaaatcgc ccaagaaatg ttggcgcatt gatgagaaat cgactagttc
    12001 ataagagcta gtcggttttt tgatactaga tggctaactt cattatagac ctttcagaac
    12061 taagggaacc tgaaaaaata aataaaactc ttgaaatatt aggacttata tgctagaatg
    12121 tagttagtgt ataaggttca ttagaacacc aaagccccta actagtgcca tagttaaggg
    12181 cttttttgac gtatcttagt gatttaacgg gtgtttatag gctagtcagt cggtctactt
    12241 cttaccatct agccatttgc agacgaaata caagataatt ccagctataa catctgctat
    12301 aatagtaaga gcaagctctg gtataaggct cattagtttc acctcctctc acgaacccat
    12361 aggaacgtaa tcggtaaccg atgacaaaaa tagtatacca cactaaattt agatcatcaa
    12421 cctcacttaa tgcttgaact attgtagatt atatgctaaa atgtagttaa ttgataaggt
    12481 tcattagaac accaaagccc ctaactatgg ccgtagttaa gggctttttt gacgtatctt
    12541 agagatttaa cgggtgttta taggctagtc attcggtcta ctccttacta tctagccatc
    12601 tgcagatgaa atacaagata attccagcta taacatccgc tataatagta agagcgagct
    12661 ctatgataag gctcattagt ttcacctcct ctcacgaacc cataggaacg taatcggtaa
    12721 ccgatgacaa aaatagtata ccacaataca tttagatcat caaggtcact taattcttga
    12781 aatatagggt ctaggcgaca aagtctttaa aatcagaaaa acgcataata tcaggtgtgc
    12841 aaaaacttga tactatgcgt tttattgtgg gaatatttgc cccatttctc ttgaaattga
    12901 gtttttgtcc agcctctgtt tttagggttg ctaagaaaat aatgtcatgt ggtgaatatt
    12961 tgtaaatcag tcagcagaca gaacgagtat tcttcgaaaa tctcttcaca tcatgtcagc
    13021 ttcgtcttgc cgtgtccatg ggggacttga cttcatcagt tttcaaatct gtactttgag
    13081 caagctgaga ctagcttcct atttgatttt cattgaatat cagaaaccca ttctccatca
    13141 aataattcga ctgcgtctaa taatttttga tctggcacgg tgtcagaaat aaaggttgtg
    13201 tatttggata ggggattaat tttaaaaaat ccagtcttgt aaaatttaga gctatcaatc
    13261 agtaagatgg tttcatgggc tttgtcaata atattctttt ttgaaatagc ttggctgaga
    13321 gaagcttcat aaacatattg gtcatcaata cctcttgctg aacaaaatgc taaatcgata
    13381 ttaaaatgat ctaataaaga attttcctta tcgtaattga ccacggaaca ggattgatgt
    13441 ttgacctcgc cagatgtgat aaagattttg gagctatctt taacagtttc agatagggtt
    13501 tgtgcagtat gtaaaccatt tgtaaaaata atcaaattat caagttcaga aagataggga
    13561 caaagttcgt agacagtcgt actggaatcc agataaacac acatacctga ccgaataaag
    13621 tctttagcga gactagcgat tagtcttttt tgcctagtac tttctccttc ccgtatttga
    13681 tgagaaagtt caattgtgtt catagaggac agggtcacgt atccgtgctt tcttttgata
    13741 agaccttgat tttctaagaa aattaaatca cgacgtaagg tacttgtgct ggagaaagtg
    13801 atttctgcca gctcttttac ggcaattctt tttttctttt taataatttc aatcaattca
    13861 agtacacgtt catcttttat cataagctcc tcctaattta tcatttcaac tatattatag
    13921 cacaaattgg aggaatttga attattttta tgaatattgg gttaacattt gaacattatc
    13981 caagtaagcg ttcacatatt gaaaaaataa aatgtgggga ttataataaa cttaatcaag
    14041 gacgaagaga gaagaaaaat ggaagcggtt ttagcaatag atttaggtgc gacttctgga
    14101 agagcaatcg ttggttacct ttctgaaaat aaactagtaa tggaagaaat aaatcgcttt
    14161 tctaatctac ctattagagt aaaagggcat ttatcttggg atattgactt tctactagct
    14221 aaaattcttg aaagtatccg cttggctaat actagttaca agattttatc tatcggtatt
    14281 gacacatggg gagttgattt tggactgatt gataatgaag gtaagctgtt atcacaacct
    14341 gttcattatc gtgatgaaag aacaaaggga gtgttaaagg aaatatctga aatgactgaa
    14401 ttagaaaaac tgtattcaga gacaggaaat cagattatgg agataaatac cttgtttcaa
    14461 ctctttaagg cacgtcaaga atctcctgac tctttctata agaccaataa
//
LOCUS       DAVKJU010000053        14160 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVKJU010000053 DAVKJU010000000
VERSION     DAVKJU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.053"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            83..880
                     /locus_tag="VW191_001557"
     CDS             83..880
                     /locus_tag="VW191_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEU9624302.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..1150
                     /locus_tag="VW191_001558"
     CDS             1028..1150
                     /locus_tag="VW191_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEU9624303.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1172..2212
                     /locus_tag="VW191_001559"
     CDS             1172..2212
                     /locus_tag="VW191_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEU9624304.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILAIAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFFDIDNLEEVEAAIKDNTKLVLIETLG
                     NPLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2383..3657
                     /locus_tag="VW191_001560"
     CDS             2383..3657
                     /locus_tag="VW191_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEU9624305.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..4545
                     /gene="truB"
                     /locus_tag="VW191_001561"
     CDS             3667..4545
                     /gene="truB"
                     /locus_tag="VW191_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU9624306.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTKTEDASGEVVAETPVLSLLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4786..5088
                     /locus_tag="VW191_001562"
     CDS             4786..5088
                     /locus_tag="VW191_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEU9624307.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5196..5834
                     /gene="udk"
                     /locus_tag="VW191_001563"
     CDS             5196..5834
                     /gene="udk"
                     /locus_tag="VW191_001563"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEU9624308.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5962..7302
                     /locus_tag="VW191_001564"
     CDS             5962..7302
                     /locus_tag="VW191_001564"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU9624309.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEEAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7280..7492
                     /locus_tag="VW191_001565"
     CDS             7280..7492
                     /locus_tag="VW191_001565"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU9624310.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7489..8364
                     /locus_tag="VW191_001566"
     CDS             7489..8364
                     /locus_tag="VW191_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU9624311.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8357..9172
                     /locus_tag="VW191_001567"
     CDS             8357..9172
                     /locus_tag="VW191_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEU9624312.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9165..9596
                     /locus_tag="VW191_001568"
     CDS             9165..9596
                     /locus_tag="VW191_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU9624313.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIDTFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9603..11270
                     /gene="recN"
                     /locus_tag="VW191_001569"
     CDS             9603..11270
                     /gene="recN"
                     /locus_tag="VW191_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU9624314.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11272..12000
                     /locus_tag="VW191_001570"
     CDS             11272..12000
                     /locus_tag="VW191_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEU9624315.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFIDQGMTEHHFIENDGVVAED"
     gene            12079..13902
                     /gene="lepA"
                     /locus_tag="VW191_001571"
     CDS             12079..13902
                     /gene="lepA"
                     /locus_tag="VW191_001571"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEU9624316.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(13983..>14160)
                     /locus_tag="VW191_001572"
     CDS             complement(13983..>14160)
                     /locus_tag="VW191_001572"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624317.1"
                     /translation="KKLAAGCSKHSFEVVDETDEVSSKHGFEVVDETDEVSNHTYSKA
                     TLTWFEEIFEEYKN"
BASE COUNT         4180 a         2590 c         3372 g         4018 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatggtttc ttcggaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaagtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt tttgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa tagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg acttgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttatcaa acaacatttt ttgtttttga
     1141 tgacacgtag gaaggtgccg atctgtttgc cttgaggaaa ccagggaaca tttatactcg
     1201 tatcaccaat cctacaacag ctgcccttga aggtggtgtt gaagcgctag caacagcatc
     1261 aggtatgact gcagtgactt atactatttt ggcgattgcc catgctggtg accatgtagt
     1321 ggctgcttcg actatttacg gtggaacctt caatcttttg aaagaacccc ttcctcgtta
     1381 tggtatcaca acaacctttt tcgatattga taatttggag gaagtagaag cagctatcaa
     1441 agacaatacc aagcttgtct tgattgaaac cttgggtaac cccttgatta atattccaga
     1501 cctggaaaaa ctggcagaga ttgctcataa acatcaaatc ccacttgtgt cagacaatac
     1561 ttttgcaaca ccttatttga ttaacgtctt ctctcatggc gttgacattg ccattcactc
     1621 tgtgactaag tttatcggtg ggcatggtac aactattgga ggaataattg tcgatagtgg
     1681 tcgttttgac tggacggctt cagggaaatt ccctcaattt gttgacgagg gtccaagctg
     1741 ccacaatttg agctatactc gtgatgtggg tgcagcagcc tttattatag ctgttcgagt
     1801 tcaattgctt cgtgatacag gtgcagcctt gtcaccattc aatgctttcc tcttgctaca
     1861 aagacttgaa acctcttcac ttcgtgtgga acgccatgta caaaatgctg agacaattgt
     1921 tgattttctt gtcaaccatc ctaaggtaga aaaggtaaat tatccaaaac ttgcagatag
     1981 tccttatcat gccttggctg agaaatactt gccaaaaggt gtcggttcaa tctttacctt
     2041 ccacgtcaaa ggtggcgagg aagaagcacg caaggtcatt gataatttag aaatcttttc
     2101 tgaccttgca aacgcggcag atgctaaatc gcttgttgtc catccagcaa caaccactca
     2161 cggtcaattg tcagaaaaag acctagaagc agcaggtgtc acaccaaact aaattcgttt
     2221 gtcaatcggt cttgaaaatg tagaagattt gattgaagac ttgcgcttgg ccttggaaaa
     2281 aatttaaagt aaaagaagat aaacagtggg cttcgactca ctgtttttga ttttccctca
     2341 ggcatgatat aatggttaaa gaagtctaga aagaggaacg atatgaacga aatcaaatgt
     2401 cccaactgtg gggaagtctt tacagtaaat gagagtcagt atgccgaact cttgtcccaa
     2461 gtgagaacgg cagagtttga taaggaacta cacgatagga tgaagcagga actggccttg
     2521 gctgagcaaa aggccatgaa tgagcaacag actaaactgg ctcagaagga tcaagaaatt
     2581 gcgcaattac agagtcagat ccaaaacttt gatacagaaa aagaattggc caagaaagag
     2641 gttgaacaga caagccatga ggctctcttg gctaaggaca aggaagtaca gctcttagaa
     2701 aatcagttgg ctaccttgcg tttggagcat gaaaatcaac tacaaaagac cctttctgac
     2761 ctagaaaaag aacgggatca ggttaaaaac caactacttt tgcaggaaaa ggaaaatgaa
     2821 ttatctttgg cttctgttaa gcaaaactac gaagcccagc tcaaggcagc tagtgaacaa
     2881 gtcgagtttt ataagaattt taaggctcaa caatctacaa aagcgattgg ggaaagccta
     2941 gaacagtatg cagagagtga gtttaacaag gttcgtagtt tcgcctttcc aaatgcttac
     3001 tttgagaagg ataacaaggt ctcttcgcgt gggtctaaag gggactttat cttccgtgag
     3061 tgtgatgaaa atggagttga aatcatttct atcatgtttg agatgaaaaa cgaagcggac
     3121 ggaacagaga agaagcacaa gaatgcagat ttttacaagg aattggacaa ggaccgtcgg
     3181 gagaagaact gtgagtatgc cgttttggtg accatgcttg aggctgataa tgactacttt
     3241 aacacaggga ttgttgacgt cagtcacgag tatgaaaaaa tgtatgttgt tcgtcctcaa
     3301 ttctttatcc aattgattgg tctcttacgt aatgcggcgc taaattccct aaaatacaag
     3361 caggagttgg ccttggttcg cgagcaaaat attgacatta cgcattttga ggaagatttg
     3421 gatgccttta agctagcttt tgctaagaac tataattcag cttcgactaa ctttggaaaa
     3481 gctattgatg aaatcgacaa ggccatcaaa cgcatggaag aggttaagaa attcctgacc
     3541 acatctgaaa accaactccg tttagctaac aacaaattgg aagatgtctc tgttaaaaaa
     3601 ttgacccgga aaaatccaac aatgaaagcg aagttcgaag cactgaaggg ggagtagaaa
     3661 gcaaaaatga acggtattat taacttaaaa aaggaagcag gaatgacctc gcatgatgcg
     3721 gtttttaaac tgcgtaagat tttgggaacc aagaaaattg gtcatggtgg aaccttggat
     3781 ccggatgtgg tgggtgtttt gccgattgcg gttggcaagg cgacacgcat ggtcgagttt
     3841 atgcaggacg agggtaagat ctatgagggg gaaatcactc tgggctattc cacgaagact
     3901 gaggatgcta gtggggaagt ggtcgcagaa acccctgttt tgtctctctt ggatgaaaag
     3961 cttgttgatg aagcgattgc tagcttgact gggcctatta ctcagattcc ccctatgtat
     4021 tcggcagtta aggttaatgg tcgcaagctc tatgagtatg cgcgtgctgg tcaggaagtg
     4081 gagcgtccag aacgtcaggt gaccatttat caatttgagc gaacaagtcc gatttcttat
     4141 gatggccaac ttgcccgatt cacttttcgt gtaaaatgca gtaaagggac gtacatccgt
     4201 actttgtcag ttgatttggg tgaaaagctt ggttatgcgg ctcatatgtc ccatttgact
     4261 cgtactagtg ctgctggctt acaattagaa gacgctcttg ccttggagga aattgctgaa
     4321 aaagtagagg ctgggcaatt agattttctc catcctttag agattgggac aggtgacctt
     4381 gtcaaagttt tcctaactcc agaagaggct acagaagttc gctttggtcg ttttattgag
     4441 ctagaccaaa cggacaaaga actggctgcc tttgaagatg ataaattgtt agccattcta
     4501 gaaaaacggg gcaatctcta taagccaagg aaggttttta gctagatcgt ttaggaataa
     4561 aaatcgggtg atagataaca attgcttgat aaaaccccat actaatagta gaatggtttt
     4621 gggaattata atattccaat tgttgcgagt tgtaggtact caaataatct atatagaaat
     4681 ttagaggtgt gaaatgaagc aatttaaaat tctttcagat aaatatttag agtccattac
     4741 aggttctgat gggaacttag gcccaggatt tggtgtgata attccatgat gcgaaatgag
     4801 tttcgagaaa gggtggagca acttcttcaa caaaaagaaa taaatgaaaa tagtgagttg
     4861 agtcacctgt ttcgtcttgc tatacaaaat ttagacagaa atgaaaaata ccaatcggtc
     4921 atggccaatt tgagtcaagg gttgtcactt tacctcatga cgcatcatta ccaggcacct
     4981 aagtctgtca ttgattttgg tttatggatt gccaaagctc ctagccagga aagagggaga
     5041 ctggctttct tgcagatgct tgctcaaacc ctacagggct ttcgttaaaa ctgtcgaaca
     5101 tcagagggaa gactctggtg ttttttgctg atgagactat aaattttgta aaaaatgtga
     5161 tagaatagac gacggataaa ataacggagg atagcatgca aaatagacca atcattattg
     5221 gagtgacagg tggttctggt ggtggtaaaa ccagtgtttc aagagccatt ttatcgcatt
     5281 ttcctgatga aaagatttcc atgattgagc atgattcata ctacaaggat cagtctcatt
     5341 tgacctttga agagcgtgtc aaaaccaact atgaccatcc ttttgccttt gatacagact
     5401 tgatgatcga gcagattaag gaattgttgg cagggcgtcc ggtggacatc ccgacctacg
     5461 actatacaga gcatacacgg agtagcaaga cctatcgtca ggaacctcaa gatgtcttta
     5521 tcgttgaggg tattttggtc ttggaggaca agcgtctgcg cgatttgatg gatatcaaga
     5581 tttttgtgga tacggatgac gatgtgcgca ttattcgtcg tatcaagcgt gatatggagg
     5641 agcgtggccg tagccttgat agcgttatta accagtactt aggtgtggtc aaaccaatgt
     5701 accaccagtt tatcgagtca actaagcgtt atgctgatat cgtcattcct gaaggggtta
     5761 gcaataccgt ggctatcgac ctgttgacga ccaagattgc aaagattttg gaagaagctc
     5821 gaaacagcaa ataatcacat gtggaggcct tgcctccttt ttctattttt ccctcataat
     5881 ggtacataaa tggagatttt taggcatatt tttggtataa taatacccat gaaagagcaa
     5941 gtaaatgaat agtaggtgga gatggaaaag tatttatcgg taacaacttt gaccaagtat
     6001 ctgaaaatga aattcgataa agacccatac ttggaacggg tctatttaac tggtcaagtt
     6061 tccaactttc gtaaacgacc tactcaccaa tatttctccc taaaggatga ccatgcagtt
     6121 attcaagcga ccatctggtc tgggatttat cagaaattag ggtttgacct ggaagaagga
     6181 atgaagatca atgtgattgg gcgtgtacag gtctatgaac caagtggtag ctactccatc
     6241 atcattgaaa aagctgagcc tgatggggtt ggggcgcttg cgattcagtt tgaacaactt
     6301 aagaaaaaat tgacagaaga aggcctgttt caagaacgct tcaagcaagc tctaccccaa
     6361 ttttctaaga gaattggggt agtaaccagc cgtagtggag ccgttattcg agatattatc
     6421 acgaccgtca gcaggcgatt cccaggtgtt gacatccttc tttatccgac caaggttcaa
     6481 ggtgaagagg ctgcggagga aattgctaga aatattgcgc gtgctaatca acgggatgat
     6541 ttggatttgc tcattattgg tcgtggaggt ggttctattg aggatctctg ggcctttaac
     6601 gaagaaattg tggtacgagc tatttttgaa tctcgtttgc cagttatctc tagtgtgggg
     6661 catgagacgg atgtgacctt ggcagatttt gtggcagatc gacgcgctgc aacgccaaca
     6721 gcggcggctg aactggcaac acctgtgacc aagttggatg tattagctca tttgcaaaat
     6781 caggaaaaac ggatggcaac ggcagtccga aatgttctat ctaagaaaca agaggctttg
     6841 aaaaaatgca gtcagtctgt tatctttaga caacctgagc gcttgtatga cggttatttg
     6901 caacgcttgg accaactgca actgcgtttg aaacaaagtt tgcgaactcg gatttctgat
     6961 aacaaacaat tagttcaagc aagaactcat caattagtac aattatcacc tgttaccaaa
     7021 atccaacgct atcaagaccg tttaggacag ttggacaagc tcttaggtag ccaaatggcg
     7081 ttagtttatg acgccaaggt tgctgaggcc aagcgacttt cggaagcttt gctcatgttg
     7141 gatactagcc gaatcgtggc gcgtggttat gctattgtca aaaaagaaga gtccgttgta
     7201 gattcggttg agagtttgaa gaaaaaagac caagtaacgc ttttgatgcg agatggtcaa
     7261 gtagaattag aggttaaaga tgtcaaaaca aaagaaattt gaggaaaatc tagcagaact
     7321 ggaaaccatt gtccaaagtt tggaaaatgg tgaaattgct ctggaagatg cgattactgc
     7381 ctttcaaaag ggcatggtct tgtcaaaaga gctccaagct acgctggaca aggctgaaaa
     7441 gaccttggtc aaggtcatgc aagaagacgg aacagaaagt gattttgaat gaaaaagcaa
     7501 gaaaaattag ctcttgtcga gtcggctttg gaagattttt atggagacca gcagtttgcc
     7561 tctagtttac gggagtctgt tctctattct attcatgctg gtggcaagcg tattcggcct
     7621 tttctcttgt tagaagttct ggaagccttg caggttacca tcaaacctgc tcacgcgcag
     7681 gtagctactg ccttagagat gattcataca gggagcttga ttcacgatga ccttcctgct
     7741 atggatgatg acgattatcg aagagggcgg ttaaccaatc acaagaaatt cggtgaagct
     7801 atggccattt tggctggaga tgccttattc ttagactcat atgccttgat tgcgcaggca
     7861 gatttgccaa gtcagatcaa ggtggacttg attgccaact tatcccttgc ttcaggtagt
     7921 ctgggtatgg tggcagggca agttttggat atggagggcg aacaccagca cttgtctttg
     7981 gaagaacttc agactattca tgccaataag actgggaagt tactagccta tcccttccaa
     8041 gcggcagcta ttatagctga attgtcacct gaaatgcagg tgaagctgaa aactgtgggt
     8101 gaattgattg gacttgcttt tcaagtcaga gatgatgtac tggatgtgac agctagtttt
     8161 gaggaaatcg gcaagacacc tcaaaaggat ctgcaggcag aaaaatcaac ctatcctgcc
     8221 ctgttgggct tggaagagtc cattgccttt tgtaaccaaa ccctggatca agctaatgaa
     8281 aaattggaag aaattgccca gcaggttccc tttgaaacag aatcgattgt aagtgtagta
     8341 gaaagtttga gaatcaatgg ctaaggaaag agtggatgta ctagcttata aacaggggtt
     8401 gtttgaaacg agagagcagg ccaagcgagg tgtgatggct ggcctagtcg tagcagtcct
     8461 taatggagaa cggtttgaca agccaggaga gaaaattcca gatgacaccg aattaaaact
     8521 caagggggag aaactcaagt atgtcagccg tggtggtttg aaactggaaa aggccttgca
     8581 ggtctttgat ttgtcggtgg atggcgcgac tacgattgat atcggggcct ctactggagg
     8641 ttttaccgat gtcatgctac agaatagtgc caagttggtc tttgcagtcg atgttggtac
     8701 caatcagttg gcttggaaat tacgccaaga cccacgagtt gtcagcatgg agcagttcaa
     8761 tttccgctat gctgaaaaga ctgatttcga gcaggagccg agctttgcca gtattgatgt
     8821 gagtttcatt tcccttagtc tgattttgcc agccttgcac cgtgtcttgg ctgatcaagg
     8881 tcaggtggta gcacttgtca aacctcagtt tgaggcagga cgtgagcaga ttgggaaaaa
     8941 tggaattatt cgagatgcta agattcatca gaatgtcctt gaatctgtaa cagctatggc
     9001 agtagaggca ggtttttcag tccttggctt ggacttttct cccatccaag gtggacatgg
     9061 aaatattgaa tttttagcgt atttgaaaaa agaaaagtca gcaagcaatc agattcttgc
     9121 tgagattaaa gaagcagtag agagggcgca tagtcaattt aaaaatgaat aaaaaagaaa
     9181 gacttgaaaa aattagaaga ttggtgacag attatcaaat cggcacgcaa gaagaaattg
     9241 tagaacattt gaaagaagca ggtatcactg ccactcaggc gacggtatcc cgagatatca
     9301 aagagttagg tattgtcaaa attcctttga gagacaacac ctatgtctac gaattaccaa
     9361 aatcaatcgt aaaaagtctg caactggctg aagacaatat cgaatcggct gaattgatgg
     9421 ataagatgat caatctccaa gttattccag gaaatacggc ttttgtaaaa gctcagttaa
     9481 tcgatacttt tgcagacaag atttttagtt gtttgactga tgatagctcg attttagtca
     9541 ttgccagaag tggaagtcta gcagaggaaa tctttgaaca agtaaaaaat tggtaggtct
     9601 atatgttact tgaaatttcg ataaaaaact ttgccattat tgaggctatt tccctcaatt
     9661 ttgaaaaggg gatgactgtc ctgactggtg aaacgggtgc agggaagtcg attatcattg
     9721 atgccatgaa tatgatgttg ggagctcgtg cgacgacaga tgttattcgt catggtgcac
     9781 caaaggcaga gattgagggg cttttctcag ttgagaatag tcgcctttta caggaaattt
     9841 ttgatgagca aggtttagaa ttaggtgatg aaattatcat ccgtcgggaa atcttgcaaa
     9901 atggtcgtag tatcagccgt gtaaatggtc agatggttaa tctgtctgtt ttgcgagcta
     9961 ttgggcaaca tcttgtagat attcatggtc agcatgacca tgaggagtta atgcgtcccc
    10021 aactgcatat ccagatgttg gatgaatttg gtgatgccgc tttttgggac ttgaaagaaa
    10081 cctatcaaac gagttttgat gcctatcgga aaatgcgcaa gcaggttctg gaagtcaaga
    10141 aaaaccaaca ggaacacaag gcacgtatcg aaatgttgga atttcaaatg gcagagattg
    10201 aggcagcaaa cttgcaggct ggagaagact tggctctcaa tcaagagcga gataaactcc
    10261 tcaaccacaa aaatattgcg gatacactga ccaatgccta cagtatgttg gacaatgaag
    10321 atttttcaag tctggccaat gttcgttcag ctatgaatga catggaaagt gtcgaagagt
    10381 atgatcctga ataccgtgaa atttcaagct ctctgtctga gacctactat gttttagaag
    10441 acattagcaa acgtttggaa gctatcattg aggaccttga ttttgatggc aatcgcctga
    10501 tgcaggttga gaatcgtttg gacctccttc atactattac tcgtaagtat ggtgggactg
    10561 ttgatgatgt tttgctttat tttgccaaga ttacggaaga atacaatctc ttgacaggca
    10621 ataatctttc gtctgaggac atggaagcag agcttaagaa gttggaagtc aatcttgtca
    10681 atttggcagg tcaacttgct tctgctcgtc ataatttggc tcagcaactc gaagctgaga
    10741 ttaaacaaga actgcaagat ctttatatgg aaaaagccca gtttcaggtt cgttttagta
    10801 agggaaaatt cagtcgtgag ggaaataaaa tggttgagtt ttatatttca accaaccctg
    10861 gagaagactt taaacccttg gttaaggttg cttctggagg ggaattatct cgtctcatgt
    10921 tagccattaa gtctgccttt tcacgtaaag aaggcaagac tagcattgtc tttgatgagg
    10981 tggatacggg agtttcaggt cgtgttgctc aagctattgc tcagaaaatt cataaaattg
    11041 gtcagcatgg tcaggttttg gctatctccc atttgccaca agtaattgcg attgcagatt
    11101 atcaattctt tattgagaag attagtaatg accattcaac ggtttcgact gttcgtctct
    11161 tgacggtcga agagcgagtg gaggaagttg ccaagatgtt ggcaggtgat gatgtgacag
    11221 aagcagccct gacgcaagcc agagaattgt tgagaaacag ggagaaataa gatgacagat
    11281 tattatgtaa ttggagatgt tcacggaaaa gctgggatgc tggaagacct tctcaaaacc
    11341 tgggatggtc agccccagtt gctctttcta ggtgatttga ttgacagagg tgaggatagt
    11401 caccgtgttc ttgaaatggt caaggacttg gtggacaatc aaggggctat ctgtttgtcc
    11461 ggaaaccacg agtatatgtt tttgacttgg ctagatgacc cagaagaaag ttatgaccat
    11521 tatcgtcgca atggtggaga tacaaccatt aactctatcc taggtcgtcc cttggatgca
    11581 ccagttgatg gagtagaaga tgccaagcgg gttgccgctg aagcgtcaga cttggtcgaa
    11641 ttcattcgtc aaatgccatt tgtagtagag acagacaagt atatatttgt tcacgcaggt
    11701 attgatttga ctttggacga ctggcatgaa acaacagatt ataaaaaagt atggcttaga
    11761 aaaccattcc acggagctga aaatcatact ggaaaaacca ttgtcttcgg tcatacaccg
    11821 gtctatggtt tgttacagca agaacgcggt acatctgagc tttggactac agaagatggc
    11881 aagattggaa tggatggagg agctgtttat ggtggtgtcc ttcacgggat tgtctttata
    11941 gaccaaggaa tgacagaaca ccactttatc gaaaatgacg gcgttgtcgc tgaagattag
    12001 tactcctagc agggtatggt cttgtcaaaa tatcaaaaac aatttataat aaatagatac
    12061 cctgaaagga agagaatcat gaacttagaa gaattgaaaa aacgacaggg gaagatccgt
    12121 aacttctcta ttatcgccca tattgaccac gggaaatcaa ctctagcaga ccgcattttg
    12181 gaaaaaacag agacggtttc aagtcgtgaa atgcaggccc agcttttgga tagcatggat
    12241 ctagagcggg aacgtggaat tactattaag ttgaatgcca tcgagttgaa ttacactgca
    12301 aaagatgggg aaacttatat tttccacttg attgacacac cagggcacgt tgactttacc
    12361 tatgaagttt cacgttcgct agctgcctgt gagggtgcta ttttggtggt cgatgcggct
    12421 caaggaattg aggctcaaac tcttgccaac gtttatctgg ctttggataa tgatttggaa
    12481 atcatgccta tcattaataa aattgacctg ccggctgcag atccggagcg cgtgcgtaca
    12541 gagattgaag atgtgattgg tttggatgcc agtgaagcag ttttggcttc tgccaaggct
    12601 ggtattggga ttgaagaaat cctcgagcaa attgtagaaa aagtaccagc accaacgggt
    12661 gatgtgacgg cgccacttaa ggccttgatt ttcgactctg tttacgatgc ttaccgtggg
    12721 gttatcctcc aagtgcgtgt catggacgga gtggtcaaac ctggtgataa gattcagctc
    12781 atgagcaata gtaagacctt tgatgtggcc gaagtcggta tttttacacc aaaagcggtt
    12841 ggtcgtgatt tccttgcgac tggtgatgtt ggttacattg cggcgtctat caagacggtt
    12901 caggatactc gtgtgggtga taccgttacc ttggcaacca atcctgcggc agaaccatta
    12961 catggttata agcagatgaa tcctatggtc tttgcgggtc tctacccaat cgaatcaaac
    13021 aagtacaatg acctgcgtga agcgcttgaa aaattgcaac tgaatgatgc tagtcttcag
    13081 tttgaaccag aaacatctca ggcacttgga tttggtttcc gttgtggatt tcttggactt
    13141 ctccatatgg atgttattca ggaacgttta gagcgtgagt tcaatattga cctcatcatg
    13201 acagctccgt ctgttattta caaagttaat ttgaccgacg gtgagtctat ggatgtgtct
    13261 aacccatctg agtttccaga cccaactaag attgcgacca ttgaagagcc ttatgtcaag
    13321 gcgcaaatca tggtaccaca ggagttcgtc ggagcagtaa tggagctagc tcagcgtaag
    13381 cgtggggact ttgtgactat ggactatatt gatgataacc gtgtcaatgt tatctatcaa
    13441 attcctcttg ctgaaattgt ctttgacttc tttgataaac ttaagtcttc gacacgtggt
    13501 tatgcaagct ttgactacga attgtcagaa tatcgcccat ctaagctggt gaaaatggat
    13561 atccttctca atggagataa ggtggatgcc ctcagcttta tcgttcacaa ggactttgcc
    13621 tacgaacgtg ggaaactcat cgttgataaa ctcaagaaaa tcatccctcg ccaacaattt
    13681 gaagttccaa tccaagcggc tattggacac aagattgtcg ctcgtactga tatcaaggcc
    13741 cttcgtaaga acgtacttgc taaatgttat ggtggtgacg tttctcgtaa gcgtaaactc
    13801 cttgaaaaac aaaaagctgg taagaaacgc atgaaatcca tcggatcagt tgaagttccg
    13861 caagaagcct tcctcagcgt cttgagtatg gatgaagaat aagttatcag aaatgccttt
    13921 ccttgttcac aggaaattta tataagaatc aatagtaaag caggcttaga tggtctgctt
    13981 ttttagtttt tatactcttc gaaaatctct tcaaaccacg tcagcgtcgc cttactgtat
    14041 gtatggttac tgacttcgtc agtttcatct acaacctcaa aaccatgttt tgagctgact
    14101 tcgtcagttt catctacaac ctcaaaacta tgttttgagc aacctgcggc tagcttctta
//
LOCUS       DAVKJU010000054        14003 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVKJU010000054 DAVKJU010000000
VERSION     DAVKJU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.054"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(21..872)
                     /locus_tag="VW191_001573"
     CDS             complement(21..872)
                     /locus_tag="VW191_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9624318.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYICNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLYEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKAFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVKILEKE
                     INDFSNL"
     gene            1199..1591
                     /locus_tag="VW191_001574"
     CDS             1199..1591
                     /locus_tag="VW191_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624319.1"
                     /translation="MKIKKLLKMVIPVLMISAVGTTFVEANQIGAFSNFVITTSYKRT
                     GYLTKENEGAEYIMNLNPCRNLHPMTVKHRIVNSNGEARSGESLTTCGTRSTHGNWAT
                     VGYVYAADMARQNWWDLSAAISGSWSPN"
     gene            1613..2404
                     /locus_tag="VW191_001575"
     CDS             1613..2404
                     /locus_tag="VW191_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624320.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYPGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVPYMVLEKYEDNV"
     gene            2397..3038
                     /locus_tag="VW191_001576"
     CDS             2397..3038
                     /locus_tag="VW191_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624321.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLVFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            3042..3674
                     /locus_tag="VW191_001577"
     CDS             3042..3674
                     /locus_tag="VW191_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9624322.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            3702..3989
                     /locus_tag="VW191_001578"
     CDS             3702..3989
                     /locus_tag="VW191_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624323.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            4135..6648
                     /gene="secA"
                     /locus_tag="VW191_001579"
     CDS             4135..6648
                     /gene="secA"
                     /locus_tag="VW191_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU9624324.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            6729..7760
                     /locus_tag="VW191_001580"
     CDS             6729..7760
                     /locus_tag="VW191_001580"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9624325.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            7762..8793
                     /locus_tag="VW191_001581"
     CDS             7762..8793
                     /locus_tag="VW191_001581"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU9624326.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            8832..9200
                     /locus_tag="VW191_001582"
     CDS             8832..9200
                     /locus_tag="VW191_001582"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU9624327.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            9190..10293
                     /locus_tag="VW191_001583"
     CDS             9190..10293
                     /locus_tag="VW191_001583"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU9624328.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVNGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHIKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            10312..12327
                     /gene="recG"
                     /locus_tag="VW191_001584"
     CDS             10312..12327
                     /gene="recG"
                     /locus_tag="VW191_001584"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU9624329.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            12643..13623
                     /locus_tag="VW191_001585"
     CDS             12643..13623
                     /locus_tag="VW191_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEU9624330.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAVYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT         4121 a         2557 c         3125 g         4200 t
ORIGIN      
        1 atgatgagag ttattacaaa ttataggttt gaaaaatcat taatttcttt ttcgagtatt
       61 ttaactaatg cctcttcttt ggttagtcgt aataaagtta ttcctttatc aatcaaatcg
      121 gagttatgtt gtagtattcc caagcgtact tgagagaatc caagaatatc gtatattttt
      181 aattcttctg ctaataccaa aagttgtagg gtgattgttt cacattcttt gaataaatga
      241 tgttgaaaat atacagtgga gagattggct aaaaaagatg ataaaaatgc ttttgtttct
      301 cggaagtatt tatatttctc gatggttttc ataattctat caataagaag gggaagattt
      361 tcagaaggaa agaaaaagag aatggttcca agcaatttca aatcactaat gtaccatgta
      421 tccataggtt caagatagtc ccaaatttct tcatacaaat ctttcaagac gtggttgttt
      481 ttgaatcctt tagttcgtaa ctctgtgaca atttttgtat gacgatagat attttgaata
      541 ggaacatcgt gatgtgtctt aagatatttt tgacatttct cagtgagttc aaccattttt
      601 ctagtatctt gaaaagtaga cggattttgg ctctctacaa taatatctcg ccttttacta
      661 ggatggtatt cgttgcatat atacttgaat tctgccaagc tcatatcaat ttgttcaaga
      721 agaaatacca tgttttcaaa actgggaata actttaccgt gttcaatatt tgttagagtt
      781 gttcgatgta tgacttgtcc acatacatct tgttgggtca atccttttga ctttcgaatt
      841 tctttaaaaa tttgtccata atcccatctc atattgttcc tcctaggtaa atgtgaatat
      901 tttctacatt tataaaaaac gtagaaaaag gtggtattat tatatcaaaa aggagggaaa
      961 aagtcatggg ctttaaaaaa tatttgaaga atttaccgaa aaactctgga tttttgattt
     1021 ggagttggat tcaacttatc tggtttgaaa catggttttg gggataaaga aattagagtc
     1081 tatatataat aagtaaagga ggaatttgta tgatatacca tcgtttagaa taagtctata
     1141 tttaaaatag aagttataag gaatcttctt gaataaaaaa tgaaaggaaa cagaaaaaat
     1201 gaaaattaag aaattattga aaatggttat tcctgtttta atgataagtg ctgttggaac
     1261 tactttcgta gaagcaaatc agataggtgc ttttagtaat tttgttatta ctaccagtta
     1321 taagagaaca ggttatttga ctaaagaaaa tgaaggtgcg gaatatatca tgaacttaaa
     1381 tccttgtaga aatttacatc ccatgactgt taagcatcgt atagtaaatt ctaatggaga
     1441 agctcgtagt ggagaatcgt taacaacctg tggtactcgt tcaactcatg gtaattgggc
     1501 aacggttggc tatgtttatg cagcagatat ggcaagacaa aattggtggg atttatcagc
     1561 tgccatttca ggtagttggt caccaaatta attcgtaaag taggtataat ttatgaagat
     1621 tcctctctta actttggcaa ggcataaatt tgtttatgtc ttgcttactt tgctttttct
     1681 tgctttggtt tatcgtgacg ttttgatgac ttatttcttt tttgatattc atgcgcccga
     1741 tctagctaaa ttcgatggac aagcaattaa aaatgactta ttaaaatcag cattagattt
     1801 tcgtattctc cagttcaatc taggttttta tcaatcattt attattccaa tcatcattgt
     1861 tttgctaggt tttcaatata ttgagctgaa aaataaagtt ttacgattga gtattggaag
     1921 agaagtgagc tatccagggt taaaaagaaa gttgactttg caagttgcaa gtatcccttg
     1981 tttgatatat ttagtgactg tgctgacaat tgcaattata acctatttcc ttgggacttt
     2041 ttctcctctt ggatggaatt ctctattttc tgatggaagt ggtttacaaa gactcctaga
     2101 tggagagata aaaagctatt tgttctttac ttgtgtcctg ctaatcggta ttttcatcaa
     2161 tgcaatctat tttttacaaa tagttgatta tgtggggaat gtgactcgtt cggcaatcac
     2221 ctatttgatg tttctttggc ttggttctat gctgctctat agtgccttgc cttactatat
     2281 ggttcctatg acgagtttga tgcaagctag ctatggggat gtaagtttga tgaaactctt
     2341 tactccttat atcctttata ttgtccctta catggtgctt gaaaaatatg aagataatgt
     2401 ttaagaattt taacaatatt ttgctaaaga gaaagattgt tttactactt cgtatagttc
     2461 tgatgatgat tttgataaac catctattgt caacagcggt tcaaaagcag gatgctgtta
     2521 tctttttcaa gagagaattg atttcaattt tttcctataa tgactattct gaagcgaatt
     2581 tagaaatccc caaactcttg ttaaaccttt cgcttttcat ggtaggatgg ctctctgtca
     2641 ttttacttga aagtgatttg gcagaccatt accatcactt gattcgctat caatcaagct
     2701 cctttttcga ttatacaagg aaacgattgg ttgtcatttc taaatttttt actcaagatt
     2761 tgtttgtctg gttccttggt ttacttccgc taggaattca tttcaaaaca gtcgcacttt
     2821 tctttttact tgctcagtta atgatgttgt acttactact gtcttatctg atagcactga
     2881 ttagtgcggg cgctggtttt tccttttttc tctatttttt agtatttgtg ggacaagaat
     2941 ggatgatgga tcatattgta acagtgtatt tagtactctt aagtttatta gttatgttga
     3001 ttgttagtcg cttggaagag aaatttaaga aaggataaac gatgagactt gaaattataa
     3061 atggacagaa aatttatggg aaaagaccta ttttaaatca gttgaatttg gtgtttcaat
     3121 caggaaaaat ttatggactt aaaggtgata atggatctgg caagacggtt cttttaaagg
     3181 tacttgctgg ctatattaag cttgacaaag gaaaagttct tcaagacggg aaagtctacg
     3241 ggataaaaaa tcattatatt caggatgcag gaattttaat tgaaaaagtc gagtttttat
     3301 ctcatttatc cctgagagaa aatttggaac tgttaaggta tttttcatct aaagttacgg
     3361 aaaaaagaat tgcctattgg attcaatact atgatttaca ggaatttgaa gacgttgaat
     3421 accgtcattt atccttggga acaaagcaaa aaatggcctt gattcaagcc tttatttcct
     3481 ctccttctat actctttctc gatgaaccta tgaatgcttt ggatgagaag agtgtgaggt
     3541 taaccaaaca ggtcatttta tcttacctga aaaaagaaaa tggtctggtt atcctgacgt
     3601 cgcacatatc ggaagatatt tcagaccttt gtacagatgt attagttgtc gaaaatggac
     3661 atatacaaat gtaaaggata tacaatccta ggagatggct tatggcacat ctaaaatcat
     3721 ttattacacg ctattccaag gtttatattg gtttagttct gctgatctgg ctgtctttct
     3781 tctttatccc ttgggataga gcgattctgg gggtaaggat tgacatcttc atcatacaga
     3841 aaatcttgct agcttttgga attctgtcca ttctcatggc cttgctgtcc aagaaagtca
     3901 gtctctttgt ttttggactg atttgctgtc tttctctttg gattaactta tttatcacat
     3961 ttgccatttt gccgattttt ggcaattaaa cagtcataaa agtcggagag gttagcttga
     4021 aaactaacct ctttttcctt ttcaaaatgg ggattcttcc ttgaaaataa tcagtaattg
     4081 tgctaaaatt aaaggaacat tctaaaatat tcggaattta aagtaaggaa aaacatggct
     4141 aatattttaa aaacaattat cgaaaatgat aaaggagaaa tccgtcgtct ggaaaagatg
     4201 gctgacaagg ttttcaaata cgaagaccaa atggctgctt tgactgacga ccaactaaaa
     4261 gcaaaaacag ttgaatttaa ggaacgttat caaaatggag aatcactgga ttcattgctt
     4321 tacgaagcat ttgcggttgt ccgtgaaggt gccaaacgtg tcctaggtct cttcccttat
     4381 aaggttcagg tcatgggggg gattgttctt caccatggtg acgtgccaga gatgcgtaca
     4441 ggggaaggga aaaccttaac tgcgaccatg ccggtatacc tcaatgccct ttcaggtaaa
     4501 ggggttcacg tagttacggt taatgaatac ctgtcagaac gtgacgcgac tgagatgggt
     4561 gaattgtact cttggcttgg tttgtcagta gggattaact tggctaccaa atctccaatg
     4621 gagaaaaaag aagcctatga gtgtgatatt acttactcaa ctaactcaga aatcggattt
     4681 gactaccttc gtgacaacat ggtcgttcgc gctgaaaaca tggtacaacg tccgcttaac
     4741 tatgccttgg tcgatgaggt tgactctatc ttgattgacg aggctcgtac acctttgatt
     4801 gtatcaggtg ccaatgcggt tgaaaccagt cagttgtatc acatggcaga ccactatgta
     4861 aaatctttga acaaagatga ctacatcatc gatgtgcagt ctaagactat tggtttgtct
     4921 gattcaggga ttgacagggc tgaaagctac ttcaaacttg aaaacctcta tgacatcgaa
     4981 aacgtggctt tgacccactt tatcgataac gcccttcgtg ccaactacat catgcttctc
     5041 gatattgact atgtggtgag cgaagagcaa gaaatcttga ttgtcgacca atttacaggt
     5101 cgtaccatgg aaggtcgtcg ttattctgat ggattgcacc aagctattga agccaaagaa
     5161 ggtgtgccaa tccaggatga aaccaagaca tctgcctcaa tcacgtacca aaaccttttc
     5221 cgtatgtaca aaaaattgtc tggtatgacg ggtacaggta agactgagga agaagaattt
     5281 cgtgaaatct acaacattcg tgttattcca atcccaacaa accgtcctgt tcaacgtatt
     5341 gaccactcag accttcttta tgcaagtatc gaatctaagt ttaaagcggt tgtcgaagac
     5401 gttaaggctc gttaccaaaa gggtcaacct gtcttggttg gtacagtagc ggttgaaact
     5461 agtgactaca tttctaagaa attggttgca gctggtgttc ctcacgaagt cttgaatgcc
     5521 aaaaaccact atagagaagc ccaaatcatc atgaatgctg gtcaacgtgg tgccgttacc
     5581 atcgcaacca acatggcggg tcgtggtacc gacatcaagc ttggtgaagg tgttcgtgaa
     5641 cttggaggac tttgtgttat tggtacagaa cgtcatgaaa gtcgtcgtat cgataaccag
     5701 cttcgtggac gttcaggtcg tcaaggagat ccaggtgagt cacaattcta cctatctctt
     5761 gaagatgatt tgatgaaacg ttttggttct gaacgcttga agggaatctt tgaacgcttg
     5821 aacatgtctg aagaggccat tgagtctcgc atgttgacgc gtcaggttga agcagctcag
     5881 aaacgtgtcg aaggaaataa ctacgatacc cgtaaacaag tccttcaata cgacgatgtc
     5941 atgcgtgaac aacgtgagat tatctatact caacgttatg atgtcattac tgcagaccgt
     6001 gacttggcac ctgaaattca ggcaatgatc aaacgcacga ttggtcgtgt cgttgacggt
     6061 catgcgcgtg ctaaacaaga tgagaaacta gaggcaattt tgaacttcgc taagtacaac
     6121 ttgcttcctg aagattctat tacgatggaa gacttgtcag gcttgtctga taaggccatc
     6181 aaggaagagc ttttccaacg tgccttgaag gtttacgata gtcaggtttc aaaactacgc
     6241 gatgaagaag cagttaaaga attccaaaaa gttttgattc tacgagtggt ggataacaag
     6301 tggacagatc atatcgatgc ccttgatcaa ttgcgtaacg cggttggact tcgtggctat
     6361 gctcagaaca accctgttgt tgagtatcag gcagaaggtt tccgtatgtt taatgatatg
     6421 attggttcga ttgagtttga tgtgacacgc ttgatgatga aagcacaaat tcatgaacaa
     6481 gaaagaccac aggcagaacg tcatatcagt acaacagcga ctcgcaatat cgctgctcac
     6541 caagcaagta tgccagaaga tttggatttg agccagattg gacgcaatga actttgccca
     6601 tgtggttctg gtaagaaatt taaaaactgt cacggtaaaa gacaataaaa tgagatagtt
     6661 tagaggcgga tatcttgtga aaagtaaatt tttactgggt atccgtttgc tttataagga
     6721 gatgagttat ggtatttaca gcaaaaagct ctaaaataaa tatagaagaa gttcgtgcct
     6781 tgtcaaaatt agaaggtcag gctttggaga ggaaatcaca gcgagatcaa gagctagaag
     6841 ccattatacg tggagaagac cagcgaattc tcttggtaat cgggccatgc tcatctgaca
     6901 acgaagaagc tgtccttgaa tacgctaagc gtttggcagt cctacaagaa gaagtggcag
     6961 atcgtatctt tatggttatg cgtgtttata ctgccaaacc ccgtaccaac ggagatggct
     7021 ataagggctt gattcaccag cctaacgcga cagaagcgcc tagtcttatc aatggaatca
     7081 aagccgttcg ccatcttcac tatcgtgtca tcacagaaac agggatgaca actgctgatg
     7141 aaatgcttta tcctgaaaac cttccgcttg tagatgattt gatttcttac atggcagttg
     7201 gtgcccgttc agttgaagac cagcaacacc gctttgtggc aagtggggca ggattttcta
     7261 ctggttttaa aaatccaacc tctggaaatc tcaatgtcat gtttaatggg atttatgctg
     7321 ctcaaaacaa acaaagtttc cttttcttag gaaaagaagt agaaacaact gggaacccgc
     7381 tttcacacgc tattcttcgt ggtgctctta atgagtatgg aaaaaatatt cccaactact
     7441 attatgacaa tttaattgat accattgccc agtatgagaa aatgggcttg gaaaatcctt
     7501 ttatcatcat tgataccaat catgacaatt ctggtaagca gtatattgaa cagatccgaa
     7561 ttgtccgcca gaccttgatt aaccgtgctt ggaatgaaaa aattaagcag ttcgttcgtg
     7621 gttttatgat tgagtcttat ctggaagatg gtcgacaaaa tgagccagaa gtatttggta
     7681 agtctatcac agacccttgc ctgggttggg ataacacaga agctcttgtc agagaaattt
     7741 acaaaacgtt aggagaataa gatggcattt attgaaaaag gtcaagaaat cgatatggaa
     7801 gtcatcaagg ctgaaaccca attgtctgca gaagccttga gactcaagga aagccgtgac
     7861 agggaattgg cagatattat ttcaggggaa gatgaccgta ttctcttggt gattggtcct
     7921 tgctcttctg ataatgaaga ggcggtcttg gaatatgctc gccgtttatc cgccttgcaa
     7981 aagaaggtag cggacaagat tttcatggtc atgcgcgtgt atactgctaa gcctcgtacc
     8041 aatggagacg gctataaagg attggttcac cagccagata cttctaaggc tccaagcctg
     8101 attaacggct tgcaggctgt gcgccagttg cactaccgcg tgattacaga gactggtttg
     8161 acaacggcag atgagatgct ttatccgtca aatctgatct tggtggatga cttggtcagc
     8221 taccatgccg ttggagctcg ttctgtggaa gaccaagagc accgctttgt ggcttctggg
     8281 attgatgcac cagtagggat gaaaaatcca acctcaggaa atttgggtgt tatgtttaac
     8341 gccatctatg ctgctcaaaa caagcaaacc ttcctttatc atgggcagga agttgagaca
     8401 tcaggtaatc ctttggccca tgttatcctc cgtggagcag tcaacgagta tggcaattat
     8461 atgccgaatt actactatga aaacctactc caagccattg aacgctatga aaccatggga
     8521 cttgaaaatc cttttatcct cattgacacc aaccatgata actcaggcaa gcaatatatg
     8581 gagcagattc gaattgttcg ccagaccttg cagaatcgtg attggaatga gaaaattaaa
     8641 aagacggttc gaggatttat gattgaatct tacctagcag atggtcgtca aaaccaacca
     8701 gagatctttg gttgctctat tactgaccct tgcctaggtt gggaaaatac agaggccttg
     8761 gtagaagaga tttatgttac cttgacaaaa taagtgaaaa ggatggagtt ggggaatctc
     8821 aactcctttt gatgagaatg atagttggac acggaattga catcgaagaa ttggcttcga
     8881 tagaaagcgc agttacacga catgaaggat ttgctaagcg tgtactgacc gctcaggaaa
     8941 tggagcgctt caccagtctc aaaggacgca ggcaaataga atatttagct ggtcgctggt
     9001 cggctaagga ggccttttcc aaggctatgg gaacgggcat tagcaagctc ggttttcagg
     9061 atttggaagt cttgaacaat gaacgtgggg cgccttattt tagtcaggca ccattttcag
     9121 gaaagatttg gctgtctatc agccacaccg atcagtttgt gacagccagt gtcattttgg
     9181 aggaaaatca tgaaagctag tccacataga ccaaccaagg ctctgattca tctgggagct
     9241 attcgacaaa atattcagca aatgggggct catatccctc aaggaacgct caagttggct
     9301 gtggtcaagg ccaatgctta tggtcatgga gctgttgccg ttgccaaggc aattcaagat
     9361 gatgttaatg gcttttgcgt ttccaatatc gatgaagcca ttgaactcag acaagctgga
     9421 ctcagcaagc caatcctcat tttaggagtt tctgaaatcg aagctgttgc tctagctaaa
     9481 gaatatgact tcaccttgac agtggctgga ctggagtgga ttcaagcact cttagataag
     9541 gaagtggacc taactggatt gacagtccac ctcaagattg attcagggat gggacggatt
     9601 ggttttagag aggccagtga ggttgagcag gctcaagatt tgctccaaca acacggtgtt
     9661 cgtgttgaag gaatctttac ccactttgct actgctgatg aggaatcaga tgactatttt
     9721 aatgcccagt tagaacggtt taaaactatt ttagctagta tgaaggaagt tccagagctg
     9781 gttcatgcta gcaattctgc aacgactctt tggcatgtag agactatttt caatgcggtt
     9841 cgtatgggag atgccatgta tggcctcaat ccaagtggag cggtcttgga tttgccttat
     9901 gatttgatac cggccttgac cttggagtct gctctggttc atatcaagac agttccagct
     9961 ggtgcttgca tgggctatgg agcaacttat caagcggata gcgagcaagt catcgcgacc
    10021 gtgccaatcg ggtatgcaga tggatggaca agagacatgc aaaatttctc tgtcttggta
    10081 gatggccaag cttgcccaat tgtcggcagg gtttcgatgg accaaatcac tattcgattg
    10141 cctaagcctt acccactcgg aaccaaggta accttgattg gctccaatgg ggataaggaa
    10201 atcactgcaa ctcaggtagc gacctaccgc gtaaccatta actatgaggt ggtttgcctt
    10261 ctcagcgacc gtattccgag agaatattat tagaaaagaa aggagtggag catgaatcta
    10321 catcaaccct tgcatgtctt gcctggtgtg ggaccaaagt cagcagaaaa atacgccaaa
    10381 ctaggaattg aaaacttgca agatctcttg ctctactttc ctttccgtta tgaagacttc
    10441 aaaaccaagc aggtgctgga actagaagac ggtgagaagg cagttctttc tggtcaggta
    10501 gtgactcctg ctagtgtcca gtattatggt ttcaagcgca atcgcctgcg ttttagtctc
    10561 aagcagggag aggtcgtttt tgcggtgaat ttctttaacc agccctatct ggctgataaa
    10621 atagagttgg gagcaaccct tgctgtcttt ggaaaatggg accgcgctaa ggctagtctg
    10681 actgggatga aggttctggc tcaggtagaa gatgacctcc agcctgttta tcgtctggct
    10741 cagggaatca gtcaggccag tctggttaag gtcatcaaga cggcttttga tcagggactg
    10801 gacctcttga tagaagaaaa tctgccccag tctttactag acaaatacaa actcatgtcc
    10861 cgttgtcagg cagtccgagc tatgcatttt ccaaaggatt tggcagaata caagcaggct
    10921 cttcgccgta taaagtttga ggaactcttt tatttccaaa tgcagctgca gacgctcaag
    10981 tctgaaaata gaattcaggg aagtggtctg gttctggatt ggtctcagga aaaagtgaca
    11041 gcagttaaag caagtcttcc ttttgccctg acccaagctc aggaaaagag tttgcaggaa
    11101 attttaactg atatgaagtc cgaccaccac atgaatcgtc tcctacaagg ggatgtgggg
    11161 agtggaaaaa cggtagtcgc tggcttggcc atgtttgcgg cagtgacagc aggttatcag
    11221 gctgccctaa tggtaccaac agaaatcctc gcagagcaac actttgagag tttacagaac
    11281 ctttttccca atttgaaact ggctctcttg acaggttcct tgaaagctgc agaaaagaga
    11341 gaagtcttgg agaccattgc caagggtgag gctgatttga ttataggaac tcacgctctg
    11401 atacaagatg gggtggagta tgctcgtctt ggtttgatta ttatcgatga gcagcaccgt
    11461 tttggtgtag ggcaaaggcg tattttacgg gaaaaaggtg acaatccaga tgtcctcatg
    11521 atgacggcga ctcccattcc acggacgctt gccatcacag cctttggaga tatggatgtt
    11581 tccattatcg accagatgcc agcaggtcgg aagcctattg tgacgcgctg gatcaaacat
    11641 gagcaactac ctcaggtctt gacttggtta gagggggaaa ttcaaaaagg ttcccaagcc
    11701 tatgtcatct ctcctttgat tgaagaatca gaagctctag atttgaaaaa tgccattgcc
    11761 ttatcagaag agctgacggc tcattttgca ggcaaggcag aggtggctct tctacatggt
    11821 aggatgaaga gtgacgaaaa agaccagatc atgcaggatt tcaaggagag aaagacggat
    11881 attctggttt cgacgacggt tattgaggtt ggggtcaacg ttcccaatgc gactgtcatg
    11941 attatcatgg atgccgatcg cttcgggctc agccagcttc accagcttag aggtcgtgtc
    12001 ggtcgggggg acaagcagtc ctacgctgtt ctcgttgcca atcccaagac ggattctggg
    12061 aaagaccgca tgcgcatcat gacagaaacg accaatggat ttgtccttgc ggaggaagat
    12121 ttgaaaatgc gtggttctgg tgagattttt ggaaccagac agtcaggact tccagagttc
    12181 caagtggctg atattatcga agattttccg attttagaag aagcaagaaa ggttgctagc
    12241 tacattagtt ctatagaagc ttggcaagaa gatccagagt ggcgcatgat tgcccttcat
    12301 ctggaaaaga aagaacatct ggattaagct ttctctaagg aaaacttaag ctagttttta
    12361 ggtttggctc ttatactaaa gtcatcaaaa agaaacgagg actctcatat gacagtaacg
    12421 attaaagtaa attaccaaac cactttccaa aagaaggaag caaaaaacta gtataaacag
    12481 aagagagagc gaaatgctct ttttcgtttc taaaactact ttcagcccat catcctaaaa
    12541 gtaaagaatc taaattcact ttctatttat ccttctttct tgcattgatt acatagatat
    12601 gctacagttg tggtaacgat tacaaaataa aaggagcatg ctatgaaaaa tccagctttg
    12661 ctagaagaaa ttaagaccta tagaggaagg gatgaggttc cggaagactt tgatgctttc
    12721 tgggatgggg aagtgaaaaa tgtttccacg cttccatcct accacttgga ggaaagagat
    12781 ttccacattc ctcaagtcaa gtgctatgag ttaacatttg aaggaagcaa ggaaggaaag
    12841 gtctatgcac gcattgttct tccaaagagt gaggagaagg ttccactaat cttccatttt
    12901 catggttata tgggacgtgg ctgggactgg gccgacatgc tgggcttcac cgtagctggt
    12961 tacggtgttg tttccatgga tgtgcggggc cagtcaggtt actcacaaga cggcttgcgt
    13021 tctcctttag gaaataccgt gaaggggcat attatccgtg gtgctgtgga aggtcgggac
    13081 cacctctttt ataaggatgt ttatctggat atttaccagt tggtcgaaat tgttgctagt
    13141 ctgtctcagg ttgatgagaa gcgtctttct agctatggtg cctcacaagg aggggctcta
    13201 gccctagttg cagcagcgct caatcctcga attcagaaaa cagttgctgt ttatcccttc
    13261 ttgtcagact tcagacgggt gattgagatt ggtaatacta gcgaggctta cgacgaactt
    13321 ttccgttatt tcaagtttca cgaccccttc catgaaacag aggaggaaat catggcgacc
    13381 cttgcctata tcgatgtcaa aaatcttgcc catcgtatcc aaggtgaggt taagatgatt
    13441 acgggcttgg acgacgatgt ttgctatccc attacccagt ttgcgattta taatcgtctg
    13501 acctgcgata aaacctatcg catcatgcct gagtatgctc acgaagccat gaatgtattt
    13561 gtcaatgacc aagtctacaa ctggctctgt ggaagtgaga ttccttttaa atatctaaaa
    13621 taaggagtcg actctaagca caaaatctta aaaattacaa acacgcatag tatcagggga
    13681 ttaaaaaaac ttgatactat gcgttttatc atggaaatat agtaaaatga aacaagaaca
    13741 ggacaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag tagatgtgta
    13801 ctattctagt ttcaatctat tatatttata gaattttttg ttgctagatt tgtcaaattg
    13861 cttaaaataa tttttttcag aaagcaaaag ccgataccta tcgagtaggg tagttcttgc
    13921 tatcgtcagg cttgtctgta ggtgttaata cttttcaaaa atctctttaa accacgtcag
    13981 cttcgccttg ccgtatatat gtt
//
LOCUS       DAVKJU010000055        13433 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVKJU010000055 DAVKJU010000000
VERSION     DAVKJU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.055"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            207..524
                     /locus_tag="VW191_001586"
     CDS             207..524
                     /locus_tag="VW191_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEU9624331.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(569..1381)
                     /gene="folK"
                     /locus_tag="VW191_001587"
     CDS             complement(569..1381)
                     /gene="folK"
                     /locus_tag="VW191_001587"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU9624332.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(1424..1978)
                     /gene="folE"
                     /locus_tag="VW191_001588"
     CDS             complement(1424..1978)
                     /gene="folE"
                     /locus_tag="VW191_001588"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU9624333.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1959..3281)
                     /locus_tag="VW191_001589"
     CDS             complement(1959..3281)
                     /locus_tag="VW191_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU9624334.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALGDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(3283..4257)
                     /gene="folP"
                     /locus_tag="VW191_001590"
     CDS             complement(3283..4257)
                     /gene="folP"
                     /locus_tag="VW191_001590"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU9624335.1"
                     /translation="MMKILEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(4352..5059)
                     /locus_tag="VW191_001591"
     CDS             complement(4352..5059)
                     /locus_tag="VW191_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEU9624336.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(5112..6530)
                     /locus_tag="VW191_001592"
     CDS             complement(5112..6530)
                     /locus_tag="VW191_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEU9624337.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            6720..7532
                     /locus_tag="VW191_001593"
     CDS             6720..7532
                     /locus_tag="VW191_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEU9624338.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            7871..8890
                     /gene="adhP"
                     /locus_tag="VW191_001594"
     CDS             7871..8890
                     /gene="adhP"
                     /locus_tag="VW191_001594"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEU9624339.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            9123..10112
                     /gene="manX"
                     /locus_tag="VW191_001595"
     CDS             9123..10112
                     /gene="manX"
                     /locus_tag="VW191_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEU9624340.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            10140..10943
                     /locus_tag="VW191_001596"
     CDS             10140..10943
                     /locus_tag="VW191_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEU9624341.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            10967..11878
                     /locus_tag="VW191_001597"
     CDS             10967..11878
                     /locus_tag="VW191_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEU9624342.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(11934..13268)
                     /gene="pepC"
                     /locus_tag="VW191_001598"
     CDS             complement(11934..13268)
                     /gene="pepC"
                     /locus_tag="VW191_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEU9624343.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
BASE COUNT         3900 a         3022 c         2656 g         3855 t
ORIGIN      
        1 tggctaactt cattatagaa ctttcaaaag taactgaaag ttatataatc aagttaattt
       61 gggaatttac ctattaactt cggtagattt ttgggaattt ttttccttct ttttccctgt
      121 agttaaaatc cttttcaaaa aatcaccttt tcttgatttt taatcctatt tgctgtatga
      181 taagggaaaa gaaaggagac agagatatgg cttttaccaa tacccacatg cggtctgcta
      241 gttttggcat tgttaccagc ttgcctgatg acatcattga ctctttttgg tatattatcg
      301 accatttctt aaaaaatgtc tttgaattgg aagaagaact cgagtttcaa ttgcttaata
      361 accaaggaaa gattaccttc cacttttcaa gtcaacacct ccctacagcc attgattttg
      421 actttaacca tcctttcgac cctcgttatc ccccaagagt actggtttta gacatggacg
      481 gtagagaaac tatcctcctc ccagaagaaa atgacctatt ttaaaaactc tagccttcag
      541 ttgcaagtaa ctgaaaacta gagtttttct attttttcaa agcatcatac aagttacgga
      601 tcggttgttt taatatcgga tggataaaat gaggcgcaat ttcctgtaag gactcaagga
      661 caaaaaggcg ttccgctatg taaggatgag gcaatatgag gtcgtctgta taaaggatct
      721 ggtcctccac aaagagcaag tccaaatcaa tcaaacgagg tccccaatgc acttctctca
      781 cccgtcccag ctctgactca atggctaaca aggtttctaa caagtcttgt gctggtagcc
      841 aggtttccac ctcaaccact tgattggcaa agctatcctg ctccactcca ccccaaggct
      901 ccgtcgctaa gacactggac tctttgagaa tatagatgcc acgagctcgc agtttgtcaa
      961 tggcttgctt caagtttgct tgtttatctc ccatattgct tcctagggcg ataaaggctc
     1021 gttgcttgcg gcgatgaatg gttaccgagc aagtatctag tgacaaatgc accggtgccc
     1081 aaggtttttt cagttccaac ttcatttctt ggacaagagg ataagactca aaggtacgtt
     1141 ccaccagttt ataggctacc gtttcaatca aatcttcact cgtttcctga aaccaagtcg
     1201 tccactgctg acacaattct ccgtaatgga cagaggctgt taaatccaag tctgtagctg
     1261 ccttggtcat atcataggat aggatggctg aaacgacaaa tttctgcccc aattctttct
     1321 cactaggaaa aagaccatga taggcaaaca tttccaaatc cttaatctgc agttgatcca
     1381 taatgattcc tttctagaaa aatccgcttg aaacggattc tttttatagc cccattaaac
     1441 gataagcttg gtcgcggagg tccttatctg tttcaaatag accacgagct actgtcgtca
     1501 aggttgcagt gcctggtttt ctaacaccac gcatactcat acacatatgt tccgcctcaa
     1561 tgacaacaaa ggctccttta gcacctagat agtccatcaa ggcatcggcc acttcgatat
     1621 tcaaacgttc ttgaatttgt ggttttttcg aataaacttc aaccgtacgg gctagcttag
     1681 acaagcctgc cacacgacca tctggaatgt aggcaatgtg cgctctacca taaaatggca
     1741 agaagtggtg ttcacacatg gtatggaaaa agatatcctt ttctaccacc atattatcgt
     1801 caataatttc aaaggatttt gacaaatgtt cctctgctgt ttgaccaaga cctgaaaaaa
     1861 tctcttgata catacgggct acacgagcag gtgtttcctg caagccctcg cgattagcgt
     1921 tctctcctac agcctcgata atcattttta cagctgcttc aatcttttgt gtatccattc
     1981 tcgttcttcc tctccatcag ataggccctc acttggctca agaaatacaa ggaacctgtg
     2041 acaatcctaa ctgtttgttt ctcttctttt ttatctgtca aattctgctc tagaaaatca
     2101 tgccaatctt ggtagctgag atttctagac ttagctgcct ctttcagcac gctttcatcc
     2161 gtcgcccgac tatccgcaaa atgtgttaga gtaagctcgg tatctggcat agctcccagc
     2221 aagtccaaca tatcccccaa ggctttggtt ttgatacaag tgaagaggat ttccttatga
     2281 taatccgcaa aacgttcttg caaggttacc aacaaggcct tgatagcatg gggattatgg
     2341 gctccatcca aaatcatcaa gggatctctt gacacgattt ccaaacgccc tggccaactt
     2401 gtttcttcca aggcttgacc aagaaagtca ttgcttgcta gctctcgacc atcttcttga
     2461 caaaaagtat caagtaaagc tatggccatc ccagcattct ctatttggta caaaccaagc
     2521 aggctagtct ggaagcgacc ttgtctgaca gcacttgtat agtcaaagac ttcccctgtc
     2581 accacacttt cttgatgacg aacctgataa tctgtcccgt aggcaagtct cggcgcatct
     2641 ttcccttccg caatgcggtc aatcacagtc aaggcttctg gagcaatacg ccctgttacc
     2701 aagggcatcc cttgtttgat aatacctgcc ttctgctctg ctatggcctc caaggtgtca
     2761 ccaagtaggg ccacatgatc caagccaata gttgtaattc ctgtcaaaat gggctgacag
     2821 acattggtac tatccaaaag tccacccatg ccaacttcca tgatagccac atctacttgc
     2881 tctgaggcaa agtagtcata ggctagggct gtgataatct caaactcggt tgtgccctgt
     2941 aaattggcga ccgcttctcc ctccagcaaa gactgatagt ctgccatgag agcttctagc
     3001 ctcgcttctg agatcgattc cccattgatg ctaatctggt ctgtgtaatg aatgagatag
     3061 ggcgaactaa acacgccaac tctcagccct agcttttcta gcatcttttt caaaaaagca
     3121 atagtcgagc ccttgccgtt agtccctccg atatggagaa ccttgagttt aagatgggga
     3181 ttgccacgca aagctaacag ttccaccatt cgttccaagc caaaatgcgg ttgatccgtc
     3241 cggtagttag caatccactg attgttttca atttctttca tcttatttat attgttttaa
     3301 atctaaattt tccgcttcat cagccagacg aatggcagag gcaatttcaa ctgccatcct
     3361 gtgactagct acgtcatgca cgcgcaccac ttctacaccc tgtctcgcag cgatactagt
     3421 tacatgagcc gaagccgtgt cccgatttcg gaaaccaagc tctgtctcag gattgacttc
     3481 aaacccactt tcttcaagga tattgatgac aaatcgcttg cgcgacactc cgagaaagat
     3541 tggatagccc ttctgatgta gtttatccag gtcccgtaaa aggagaagat tttctttctt
     3601 ggtcagacca aagccaattc ctggatccaa caggatattt tctggtgcaa taccagcttc
     3661 tgccgctctc gctagtgctc gttcaaagaa agccaccatc aagtcttcga ttggcaatgt
     3721 ttcaaagtca gctaactctt tttctgtaaa ggtttgacca aagccaaaat gagggaagat
     3781 aagcgaacta ggatgctgag gtcgagccat aactgggtta aacatgatga ccactttcgc
     3841 tccagcttta gcaaccacat gggccatttt ttcatcaccc atgagaccag tgatatcatt
     3901 gactagatcg gcaccagcag ccaaagcagc ctctgctact tgactcttcc aagtatcaat
     3961 agagatgagt acatcacttt ccttgcgaat cgctttgatc actggaacaa cacgctggat
     4021 ttcctcttct atctcaacat agctacttcc cggccgagtc gattctccgc cgatatcgag
     4081 catactggct ccttctgcta tcaatttacg agcctgctgg agcgcctgct caagagcaaa
     4141 aaattgacca ccgtccgaaa aggagtctgg ggttacattg ataattccgc aaatagctgt
     4201 ctttgcatga ttggctttac ttgacatatc ggtcactccc tcaaggattt tcatcatatt
     4261 atttttctat tttaccataa aaagaaaaag atggacacga ttgcattcat ctttccctag
     4321 tagaaagagg tcggttccta cataaaaact tttaaacaga aattcctaat gtccgactca
     4381 taatcaccac aagagccaac aaacagaaag caatcccatt aacaatcatg tgaagcaaga
     4441 tcgacatttc caaacgttgg gtcttgtagg ccgtccaaga tagaactgtc gacatacctc
     4501 cataaatcaa taaagaaggt aaattacttg gttgatgcaa taaagcaaac acaatcgtac
     4561 cgactacaaa tcccaagttc tccttgcctc ggaaaatctt tttaggaaca atcccacggc
     4621 acaagatttc ctcacaaatc ggagcaagca aggctagcaa gaagaaactg gaaatcaacg
     4681 aactattttg aaccatatcg ttaatctgag actggttagc tgttgtcgtc tcatttgaca
     4741 gttgcaacaa gatagaacca agtatatttg acccgacaat aactaaataa ctcaagccca
     4801 aacgtgccaa atctttagct ctaaaaaaag aaaaattaaa actagctaac ttggttttac
     4861 gagctcccat aataaataga gccagaacca caattgaaag gccagcaact atcagccctg
     4921 actgtagcag ggctacttct tttaaagtca aaatagaggt aaccactagg ggaacctgat
     4981 ataaaaggac ggcaagtaaa aagaccaaaa tccagcctgc acgattcaac aattctttcc
     5041 acatattttt ctctttcatc acctatctcc tttgtattaa aaaagggggg aaatccccct
     5101 gggtcattct attataaggc catgctgatg tagttaagga taaacaaggc atccaaaatc
     5161 caaatcatgg catgaacatc tttaacttga cctttgacaa gcttagtcaa agtgtaagtc
     5221 aagaaaccaa ctgcaatccc ttgagtgata gagtagctaa atcccataaa gatagatgtg
     5281 aagaaggcag gaactgcttc agacatatca tcccaatgga tatttttcaa gctaccaagc
     5341 atcataatcc caacgataat caagattgga gctgtagccg ctgttggtac gatcgctaga
     5401 agtgggctaa agaagcttga aatcgcaaaa cagatagcta caaccaaggc tgtcaaacca
     5461 gtacgtccac ctgcaccgat accagcagca gactcaacat aagtcgttac gtttgaagta
     5521 cctgcaatgg caccgactgt cgttccaatc aaatcagagt aaagagcctt atccaatttg
     5581 gctgattggt gattttcacc atttgtcgct acaataccaa ctttttcacc tgtaccgatc
     5641 aaggtaccaa ttgtgtcaaa aatatctgtc aatgagaagg caagaatggc catcagagtt
     5701 tcaggcaagc gagctgtatc tgaaaccaaa gctcccaatc cttctgaacc aagagctgca
     5761 ccaaagattg tcttcaaatc ttcaaaagct gcaccaacat ggttattagc aaaatcgata
     5821 ctagacaaat caaccaaacc aactgcaata gcaagaacag ttgttgtcaa gatagagaga
     5881 ataattcccc ctttaatccc tttgatgaca aagaagatag taatggcaag tcctgcaaga
     5941 gccactaaaa cagctggatt attaaagctg accaatcctg gaactgctga agagtttgct
     6001 gcaatcgttg cttgagcttt gtcagcccct tctcctacaa cagtatagtt gcctggatca
     6061 atcgtgaatt tcaaaagtcc agcattctta atccctacat aggcaaggaa gacaccgata
     6121 ccagctgaaa tagctgagcg aagagcattg ggaatcgatt caatgatcat tttacgaaca
     6181 tttgtcaagg taataatcaa tgaaataatc ccacagatga agaccatagc tagggcttct
     6241 tgccaagaat aaccaagccc gaatacaact gtaaaggtaa agaaggcatt gagtcccata
     6301 cctggcgctt gggcataagg taagttagca taaaaagcca tcatcaaggt acccgctact
     6361 gcaccaataa tcgtcgctag gaagacgccc tgagcaggca ttcctgtttg tgaaagtatt
     6421 tgtgggttta caaagagaat atagctcatt gcaaagaaag ttgttaaacc agcgagaacc
     6481 tctgtacgaa cgtctgtacc gttctctttt agtttaaata atttgtccat catcaatctc
     6541 cttttaattt ttacgaacaa taatagaatt atatcattta tcattcactt tttcaatgtc
     6601 tttgtttgat ttgtttaaga aattaatcca atttcatttt ttgtttggaa tctgcttttc
     6661 tgcctgcttt ctgttataat agaagacatc ttatctgaaa acacactaga aaggtccata
     6721 tgacgaaaaa aattattgca gttgacctgg atggaaccct gctcaactca gacagtcaaa
     6781 tttctgactt taccaaaaga accatcaaaa aagttgctga aaaaggccat caggttatta
     6841 ttacgacagg tcgcccttac cgtatgtcaa aagattttta ccgtgaactg ggcttagaca
     6901 ctcctatgat taacttcaac ggatccctta ctcatttacc agaccaagtt tgggattttg
     6961 aaaagtgttt gactgtagac aaaaaatatc tgctagatat ggttcaacgt tcagaggaca
     7021 ttcaagccga ttttatcgct ggagaatatc gtaaaaaatt ctacattaca aatcccaatg
     7081 aagaaattgc caatcccaaa ctatttggtg tagaagcttt ccagcctgaa gatcaattcc
     7141 agcctgaatt ggtgaccaag gaccctaact gtatcctctt gcagaccaga gccagtgaca
     7201 aatatgcctt ggcaaaagaa atgaacgcct tctaccagca tcaactttct atcaatacct
     7261 ggggaggtcc gctcaatatc cttgaatgta ccccaaaagg tgtcaacaag gcctttgctt
     7321 tggactactt gctcaagata atgaatcgtg acaaaaaaga tttgattgcc tttggagatg
     7381 aacacaatga taccgaaatg ctcgcttttg ctgggaaggg ttatgccatg aaaaatgcca
     7441 atccagagct actcccttat gcagatgagc aaatttccct taccaacgac caagatgggg
     7501 ttgccaaaac cctacaagac ttattcttat aacctatact gatactcaat gagaatcaaa
     7561 gagcaaacta ggaagctagc cgtaggttgc tcaaagcact gctttgaggt tgtagatgaa
     7621 gctgacgtgg tttgaagaga ttttcgaaga gtatgaccag cttgcaagct ggtctttgtg
     7681 ctcttttcac agtctcatca accagttaat cgattgttct actttttgtc tccaaaacct
     7741 tggaaaaggc tttcttttgt gatatacttc acatataaat acaagagagc tcgtcacact
     7801 cgactctgtt gacaaaaaat caatccagtc ccgttgtccc tgttctcggt caatatcaag
     7861 gaggatagct atgaaagctg ttgttgtaaa tccagaaagc actggtgttg ctattgaaga
     7921 aaaagtactc cgtccacttg aaactgggga agcacttgta gaagttgaat actgtggtgt
     7981 ttgccacacc gacctccacg ttgctcatgg tgactttggt caagtcccag gacgtgttct
     8041 tgggcacgaa ggtatcggta tcgttaaaga gattgctcca gatgtgaaaa gccttaaagt
     8101 cggtgaccgc gtcagcgttg cttggttctt tgaaggatgt ggcacttgcg aatactgtac
     8161 aactggtcgc gaaacccttt gccgtacagt gaaaaatgct ggctactcag tagacggtgg
     8221 tatggctgaa caatgtatcg taactgctga ctatgctgtc aaagttcctg acggacttga
     8281 tccagcccaa gcttcttcta tcacatgtgc tggagtaaca acctataaag ctatcaaaga
     8341 agcaaaagtt gaaccaggcc aatgggttgt tctttacggt gctggtggac ttggtaacct
     8401 cgctgttcaa tacgctaaaa aagtattcaa tgctcatgtt atcgcagtcg atatcaacaa
     8461 tgacaaactt gcccttgcaa aagaagtagg cgctgacatt gtgattaacg gcctcgaagt
     8521 tgaagatgta gctggactca ttaaagaaaa aactgatgga ggagctcatt cagctgtcgt
     8581 aactgctgtg tctaaagttg ccttcaacca ggctgttgac tccattcgtg ctggtggtcg
     8641 cgtcgtcgct gttggtcttc cttctgaaac gatggaactc agcatcgtta aaacagtcct
     8701 cgatggaatc caagtcatcg gttctcttgt cggaactcgt aaagacttag aagaagcctt
     8761 ccaatttggt gcagaaggtc tggtagtccc agttgttcaa aaacgtccag tagaagatgc
     8821 tgttgccatt ttcgacgaaa tggaaaaagg ccaaatccaa ggacgtatgg tactcgactt
     8881 cacccactaa tctaatagaa acctatttta aaagttggaa gcttcttcca acttttttat
     8941 attaaatttt aaataagaat tcaagattac tcctcaaaaa ctcaacatat caacatacaa
     9001 aaataaaaat tttttaattt tttatgataa atagttgctt tttttatgaa aacggtttat
     9061 acttaaacag tcttaaagtt ttcttaaatg aaaactaaaa caaaaaagga ggaatgacaa
     9121 taatgagtat cggaatcatt attgcgagcc acggcgaatt tgctgcgggt attcatcagt
     9181 caggatctat gatctttggt gaacaagaaa aggttcaagt tgtgaccttt atgccaaatg
     9241 aaggtcctga tgatctatac gctaagttta ataacgctgt tgctgcattt gacgcagaag
     9301 atgaggttct agttttggct gacctttgga gtggttctcc atttaaccaa gctagtcgcg
     9361 tgatgggaga aaatcctgag cgtaagtttg ccatcatcac aggacttaac ttaccgatgt
     9421 tgattcaagc ctacacagag cgcctcatgg acgctgctgc aggtgtagaa aaagtcgctg
     9481 ctaatatcat taaagaagcc aaagatggca tcaaagctct tccagaagag ctaaatccag
     9541 ttgaagaagt tgcaagcgct gcagctgctc cagttgccca aactgctatc ccagaaggaa
     9601 ctgttatcgg agacggtaaa ttgaaaatca atcttgcccg tcttgacaca cgtctacttc
     9661 acggtcaggt tgcaactgct tggactccag attcaaaagc aaatcgtatc atcgttgctt
     9721 cagataacgt ggctaaagac gaccttcgta aagaattgat taaacaagca gctccaggta
     9781 atgtcaaggc taacgtggtt ccaattcaaa aactgattga gatttcaaaa gacccacgtt
     9841 ttggagaaac acatgccctt atcttgtttg aaacacctca agatgccctt cgtgccatcg
     9901 aaggcggcgt gccaattaag actcttaacg ttggttctat ggctcactca acaggtaaaa
     9961 cattggtcaa taccgttttg tctatggaca aagaagatgt tgctacattt gaaaaaatgc
    10021 gtgacttggg tgttgaattt gatgtccgta aagtaccaaa tgattctaaa aaagatttgt
    10081 ttgacttgat taacaaagcc aatgtcaaat aagccattat ttatgaaagg attttaaaca
    10141 tgtctattat ttctatggtt ttagtagtcg ttgtagcctt ctttgcaggt cttgaaggca
    10201 tcctcgacca gttccaattt caccaaccac ttgtagcctg tacccttatt gggcttgtaa
    10261 caggtcactt ggaagcaggg attatcctcg gtggatcgct tcaaatgatt gcccttggtt
    10321 ggtcaaatat cggtgctgct atcgctcctg atgctgcact tgcttctgtc gctgctgcca
    10381 ttatcatggt tcttggtggt gactttacca agactggtat cggtgttgcc caagcggttg
    10441 ctatccctct tgccgtagct ggacttttct tgacaatgat tgttcgtaca atttcagttg
    10501 gtttggttca tactgcagat gctgccgcta aaaaaggtga cttcggcgct gtggagcgtg
    10561 cgcatttcat tgcgctactt ttccaaggac ttcgtatcgc gcttcctgca gctcttctcc
    10621 ttatggtacc aactgaaact gtacaaagta tccttagtgc catgccagac tggctcaaag
    10681 atggtatggc tatcggtggt ggtatggtcg ttgccgttgg ttacgccatg gttatcaaca
    10741 tgatggcaac tcgtgaagta tggccattct tcgctcttgg tttcgttctc gctgctgtgt
    10801 cagatattac tctaatcgga ttcggtgcta tcggtgttgc tatcgctctt atctaccttc
    10861 acctttctaa aactggtgga aatggtggcg gaggagccgc aacttctaac gacccaatcg
    10921 gcgatatcct agaagactac taagataaga aaggactgaa aacatcatga ctgaaaaact
    10981 tcaattaact aaatcagatc gtaaaaaagt ttggtggcgt tcaaccttct tacaagggtc
    11041 ttggaacttt gaacggatgc aaaacttggg ctgggcttat acactcattc cagctatcaa
    11101 aaaactctat actaaaaaag aagatcaaat cgctgctctt gagcgtcacc ttgagttctt
    11161 caacactcat ccatacgtag ctgctccagt catgggggtt actcttgcgc ttgaagaaga
    11221 acgtgctaac ggtgtggaaa tcgatgacgc tgctatccaa ggggttaaaa tcggtatgat
    11281 gggacctctt gctggtatcg gtgacccagt attctggttt acagtacgcc caatccttgg
    11341 atctctcggt gcttcacttg cccttactgg caatatcttg gggccactcc tcttctttgt
    11401 tgcatggaac ttgattcgta tgtcattctt gtggtatgtt caagagattg gatacaaggc
    11461 tggatcagaa atcactaaag atatgtctgg tggtatcctt caagatatca ctaaaggagc
    11521 ttctatcctt gggatgttca ttcttgctgt ccttgttcaa cgctgggtaa atattaaatt
    11581 tgctttcgat gtttctaaag ttcaactaga tgaaaaggct tatatccatt gggataaatt
    11641 gccagaaggg tctaaaggta tccaagaagc attcgcacaa gtaggacaag gattgtctca
    11701 aactcctgaa aaagttacta ctttccaaca aaacttggat atgttgattc ctggattatc
    11761 aggactactc cttactttac tttgcatgta cttacttaag aaaaaagtat ctccaatcac
    11821 tattatcctt gccctcttcg cagtgggtat tgtggcacat gttcttcaca tcatgtaatc
    11881 aagcaactga aaaggaacca ggttctaaaa tctgattcct tttttctatg cttttattca
    11941 gccaaggctc ccattggatc ccatggtgca agtacgattg gttctgctcc ataggcagct
    12001 tgttcttctg ctgtcagcaa ttccttacga acaacgattt ggtatgtgta ttcgtccatc
    12061 caagcgtctg aggcaacaaa gtaaccatct gtaccgacct tgtctcccca tgagttttca
    12121 accttccact tggttgattt accattttcg tccaagtcaa cacctgtcaa gaccatggcg
    12181 tgggtcatca agctttcact atagtccaaa cgtccagcct tgtcttgagt aagtttaatg
    12241 tccatgcttg attcaaagtc ataaacatct gtcgcaagga tgccagcctt acggttgctg
    12301 agctggccga catcagaacc aaaccaaaca gtctcacctg cttgcatttg ggcaatcgcc
    12361 aattctttca agcgctccat tggaacgttg atgtagcgaa ctgcacggct accaaccaca
    12421 ttccccaaca tctcaactgt gtaagatttt ccgtaaggtt tatcagcagt tggagcattg
    12481 ataacagaaa cgtagtcttc taaaggaaga ttgacatatt tcttgtaaaa ctcttgtggt
    12541 gtgattcctt tttcactttt gtagttgtta tctttatcgc gataagcaaa gtcaaacttg
    12601 cgtggtggaa gtcctaatga catagcaaga aagttaaaga tttcttgcaa gaggtcttct
    12661 ttcttagctt gaacagtcgc ttgatctgca ccagaaacaa gcaagtcacg caagatttga
    12721 gcatcttgac gaagcaattt attaaggatc gcatttagct cacgactgct gctagatgaa
    12781 acagactcag gataaactga cttaggcacg acaccgtatt tttcaaagag ggaaacgacc
    12841 atatcccatt gaccaccatc ttgttgaggt gtttggagta agaagctaac cttgcggcta
    12901 gtcaattctt ggtctgaagt cgcaatgact tgctccaaga accagtttga tttctcatac
    12961 ttatcccaga agaaagtgtg ggcttgtgac aactcaaagt tctccaattt gtattgcgag
    13021 atgagtttgt ggcggaaggt gttgagagcc gcaaacatcc agcaacgacc agacgctttc
    13081 tggttagtga ccttgtcctt ggttaaatcc aatgagaaaa caggtgtgtt gtctacatgg
    13141 ctttggcgac attccagagc tgcaaaaatt ccgttgtggc tggcagcatt ttcaatcgct
    13201 tggtatttta catttgcttc atagttggca aatagtttat cagtaaatga ttcttgaatc
    13261 gcgttcatag attcctcctt ttattctaca gtctattata actcttttca caaaggaagc
    13321 aactaaaaaa cataaaaggt aaggacattc tccctcacct ttttcgaatt tatactcttc
    13381 gaaaatcaaa ttcaaaccac gtcaacgtcg ccttgccgta ctcaagtaca gcc
//
LOCUS       DAVKJU010000056        13262 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVKJU010000056 DAVKJU010000000
VERSION     DAVKJU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.056"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            200..376
                     /locus_tag="VW191_001599"
                     /pseudo
     CDS             200..376
                     /locus_tag="VW191_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            559..765
                     /locus_tag="VW191_001600"
     CDS             559..765
                     /locus_tag="VW191_001600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9624344.1"
                     /translation="MREYRTYEEIAADFGIHESNFIRRSQWVEVTLVQSGFTVSRTPL
                     SSEDTVMIDATEVKINRPKKTISE"
     gene            791..1180
                     /locus_tag="VW191_001601"
     CDS             791..1180
                     /locus_tag="VW191_001601"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEU9624345.1"
                     /translation="MKAQAIVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILA
                     DSGYQGLMKIYPQAQTPRKSSKLKPLTVEDKACNHALSKERSKVENIFAKVKTFKIIS
                     TTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(1232..1594)
                     /locus_tag="VW191_001602"
     CDS             complement(1232..1594)
                     /locus_tag="VW191_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624346.1"
                     /translation="MPKTKTLAKLADVILWSFDFANDHAHAFFMDNVEWSHADSYFFS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1948..3828)
                     /locus_tag="VW191_001603"
     CDS             complement(1948..3828)
                     /locus_tag="VW191_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU9624347.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKINTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RHAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3822..4691)
                     /locus_tag="VW191_001604"
     CDS             complement(3822..4691)
                     /locus_tag="VW191_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU9624348.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4774..7419)
                     /locus_tag="VW191_001605"
     CDS             complement(4774..7419)
                     /locus_tag="VW191_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEU9624349.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDMATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7510..8790)
                     /locus_tag="VW191_001606"
     CDS             complement(7510..8790)
                     /locus_tag="VW191_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEU9624350.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFVELDLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8961..11045
                     /locus_tag="VW191_001607"
     CDS             8961..11045
                     /locus_tag="VW191_001607"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEU9624351.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNPLMLFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDVAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11085..12764)
                     /locus_tag="VW191_001608"
     CDS             complement(11085..12764)
                     /locus_tag="VW191_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU9624352.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQVLPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
BASE COUNT         3723 a         3125 c         2686 g         3728 t
ORIGIN      
        1 gtagaataga gtagtacatt ttataaattg tcatctctcc ataaacacag tatatcattt
       61 ttaaatttta agtcaaggaa aattaaagtg ggttaaaaaa gaaattaact gagtaaaaac
      121 tctcgaaatt caattcgcta tattatcact caacttgaac aaataccaga accacaaaaa
      181 acagatgtaa aggaaaaata tggacttaaa atttgaaggg gttgatttag aatacaaaaa
      241 agctaaaaac aatcttccag agtctttctg ggaaacctac tcagcatttg caaatacaaa
      301 tggcgggaaa ataattctcg ggatagatga aaaaaacatt gacacctacc aaagagtcaa
      361 tagactccct gcgaaacaaa attatgaagc aagtaaacaa ctaaccgatg cacgatttaa
      421 gcgtcttgtt ggtgttcaac gcacgacttt tgaagagatg ttagctgtat taaaaacagc
      481 ttatcaactt aaacacgcaa aaggtggacg aaaacctaaa ttaagcctag aagaccttct
      541 tatgcccact cttcaatagt gcgagaatat cgaacttatg aagaaattgc ggctgatttt
      601 ggtattcacg aaagcaactt tatccgtcgg agccaatggg ttgaagtaac tcttgttcaa
      661 agtggtttta cggtttcaag aactcctctc agttctgagg acacggtaat gattgatgcg
      721 acggaagtaa aaatcaatcg ccctaaaaaa acaattagcg aatgattctg gtaaaaagaa
      781 atttcacgct atgaaggctc aagcgattgt cacaagtcaa gggagaattg tttctttgga
      841 tatcactgtg aactattgtc atgatatgaa gttgttcaaa atgagttgca gaaatatcgg
      901 acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg ctcatgaaga tatatcctca
      961 agcacaaact ccacgtaaat ccagcaaact caagccgcta acagttgaag ataaagcctg
     1021 taaccatgcg ctatccaagg agagaagcaa ggttgagaac atctttgcca aagtaaaaac
     1081 gtttaaaata atttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa
     1141 tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga agtctattga
     1201 aatactaagc ctacacagac tgatttcaat tttaccattc ttcatagtca aaaccatcat
     1261 aatctggata ttgttctggc gacgttccaa ccgaacgtac gagataagct tcttcgtcct
     1321 ctgtctcgat ttctctcagc acttggcatt caaaacgcca ttcatcaccg aagtcgaaaa
     1381 taaacttaaa tttttgtttg acacttaggc tatccagata gacattttct gtgtaacgtt
     1441 cttcaacatc gtcactaaca aagctaaaaa agtaagaatc tgcatgactc cactcaacat
     1501 tatccatgaa aaatgcgtga gcatgatcat ttgcaaaatc aaaactccat aaaatcacat
     1561 ctgctagttt cgctaacgtt tttgtttttg gaatctatat atgtcggtag caaccttttt
     1621 taaatttgac ggaaatgacc caagcttcta tttcttgttt tactaatttt aagtgtagca
     1681 taatacctcc aattgctcat cgatagaatt gctcttagga aacattcact ttctgctatt
     1741 ttatcataag tttgtctaac tgtagcattt gaaaccagat aattcttctg tttcacgcga
     1801 aacaccctgc gccaagcaag gtgttcttgt ttttatactc aatgaaaatc aaagagcaaa
     1861 ctaggaagct agctgcaggc tgctcaaaac actgttttga ggttgtagat ggaagctgac
     1921 gtggtttgaa gagattttcg aagagtatta agtcgtataa atcgtcgacg ctgtcgcaat
     1981 ggtatgccac tggttagctg tagttgctgc gaagtccttg tctgcgtaga agtcagtaaa
     2041 tggtaggatt tcaacttcca gctcgtcgat gcggtcaagc tgaccagcca gataaacctc
     2101 gatacggctt tctgctcgtt tgatgcgttg caagagtccg cccatacgga tgtcaactgt
     2161 atccaaacca aagaccttgt tttctttcag ccattggtgg ctaaagaggg catggaagtc
     2221 ttcaatttgg cttctaagtt ctggtaattc ttgtctggcg atttgttgta aactttcttt
     2281 atcatccgct tggtaggcat gacgaatgcg tcgtcccaca tctactttgc tacttaaaat
     2341 agcattcaac tgggcctgag tttcaaagag ataagcatag tttccagctt tttctttaat
     2401 gttagcaagc gtctcagcag cctgagcgaa gtgcggtttg tcctgttcag gtgtcatgtg
     2461 ttgatcaaga atcggacaaa gaatatcctg ataaaaaaca tagcggttgg gattgatacc
     2521 gctgagattg cctggtaggt ctggtaagag gttggcaagg tcaatctgca taaaatcctc
     2581 aaccgttaga ccagtattga tcttgaagtg cgcagacaaa ccatctaggt cattgcgata
     2641 gctgagttct gcccagattt gcaagcttgg taggatagag aactgggcag tttcaccacc
     2701 attgtctccc caacccgtta cgatgacttc tttaatctga ttggcacggc aggctttatt
     2761 agcctcgata gccactagac ggctaaaatg gttgtgaggt gtaaagccaa tccacttcca
     2821 agctccccct gcaaatgcaa ggtcatggct aatcttgtga tgattgcgga aattacggtt
     2881 gtatttttcc tcgctatcct gataataatc ccagtaaacc agagtcacac ggtctttgag
     2941 acggtctagg tagacacgag tttcctctgg aatttccaca tcacgatcgt actggccatc
     3001 cgctgacatg agtttgaaga acatatcact ccacatctgg cagtggaaac catatttgtc
     3061 agcaatatcc agcacgcgct ccaagtgttg gcacatgagg agactacgat ccacaacacc
     3121 gttcaaaatc aggtagcgtc ccaaaccaac caagtgggct tcgtccatcc cgatattgac
     3181 cttgcgagtc ttcagtttag acaacgtggc aaacatgcca tcaatcaggt cataaacctt
     3241 ttcttcgcca atgagaagaa tgtcctctac atcacggagc tcctgcactt ccttgacacc
     3301 ccatttgaca aaggccgaca agtgggccaa ggtctggatg catggtacaa aggtcatgtc
     3361 aaactgttga gcataggctt cgatttcctg caactcctct gctgaataag ctccacggaa
     3421 atagccaaag taaggctgcc cttcaatctg gtaagtgtct tccatgtaaa gctcaaaggt
     3481 tgagtagccc atgagagcca agacctcaat catctgcttg gcagaagcca cattcagcac
     3541 cgcatttcga gaacagtcaa ccatgtaagc caaatcttcg taagctgctt gttcctcaat
     3601 ctctacttta tcagcttctg ctagaactgt taccaacaag gataaggcac gataaagttg
     3661 gtgaggtttg cggtaggtca attgatagtg accttcctca ccctggatag agatagaggc
     3721 ttggtcagac tgagtgacag ccacttccac atctggtaga gaaatgtgac cttttaaaac
     3781 ctcaatagct tgcgtttgtt tgggactaag tcctgtaaat cttaccattg cttttcctcc
     3841 tgtagccagt tgacaagggc accgtagaga tttgcatctg cgtgataggt gcaggcctgg
     3901 ataactgggg cgaccgtgta ttcttcgtag gtatcgacaa agttatcaac agccttcttg
     3961 acaccttgga taaaatctgg attttgactg atagatccac ccagactgat gacatctgga
     4021 tcgatcagat actggatatt gagcaagcct tgcgccagat tgcagttcat gcgctcaatg
     4081 gcttcttgac aaagagcatt accagctgcg gcctcttggt aaatcttgcg accgtcccaa
     4141 tcagtatgac cagatttttc aattacgtat cgtaccatat tcccagttga tgctagttgc
     4201 gaccagttat taagttttcc agcaggggca agggttgtca tgtagccaaa ttctccaccc
     4261 agaccgtggc gacctcgatg aagtctacca ttgataatca tggctccgcc aatccctgtc
     4321 ccaatcacga cacaggctgc attttcaagc tctggatgag ctagtagttc actgagtcca
     4381 acgcagttgg catcattttc taaatggaca ggtagctgat aagagctaag agcctcatac
     4441 caagaaaagc catggatgta gggcaccgca ctgaagccat caatcacacc tgtctcttga
     4501 ttgactgcac ctggaacgct catagcaatc ccactgtaat cctgttctga caagcgttga
     4561 tctagccacg ctagtaaatc ctccaagttt tcaggcgttg aaatacttgt cttatccagt
     4621 attttcccat caggagtcag actggcaaac ttaatcccag tccctccgat atcaatcgtt
     4681 gcaatggtca ttgtttttac cataaaaaag gcgaatcagc agttagttct tactttttcg
     4741 cccctccttt cttttgatgt ttactttttg aaattaaatt tcttcttttt tgatgaattc
     4801 tgtacgaatc tcttgtggag ccaatagtcc tgaatgaact ggatatggtc gctcaagtaa
     4861 gtcaagaatg gtttgttgat gttctgatat gcgcacattt tcttgactca tattatagta
     4921 acggaggaca tagccttctt cattttccgc cactttaaag gctgttggac aaacttgcgg
     4981 tatgctgaga acagaatggc tcaagaggct accagtcgca accacgcttc cttcctgtct
     5041 agcaagctga aggctggtaa acggtgtctg caaggctttg gcacgacgat aggctgagaa
     5101 gcgttcttgg gcttggtggc attcaagtgc aaactcgact tcaaactcac gcaagcattg
     5161 tgcttctggc gttgggaagt agccccagtc acctagctca cctgatgcac gcaaaatggt
     5221 cacggcaatg gtgttatccc caaggatttc gtattcattc aatcccttgt tggatacagt
     5281 cacccctttt tcatcgtcat acagactgac aaaagcttgt tggtgttgag gattttcagg
     5341 attttcccat gaagcagctg gtttgtttgg tcgtgtcacc acctcataga tactttcaga
     5401 atcattgctt ggacgcgtgt tatgagtctt gaccaagaga cggatacggt gatccttggc
     5461 agtgttagta aagcgagtct tgaagcggat ttgtggattg tcaacgaaga cagtcaactc
     5521 agtttccaga ggaatgtttg tcaattcttc tgaccgtcca gcctcacgct tcataaactc
     5581 gatgatacct tgttgctctt cttctagctt ttcatccgca ctgacaggca cggtcaattc
     5641 atgtttgagc aagattttag catagcgatc tgtgttttcc aatacctcgt ggcccttaag
     5701 ctctgcaaag attggctctg ttccttttgg ttggaaatag atatactcgt ttccgatgtc
     5761 accccggtct tcaaagcgga taaagtcttc ataggcttcg tgagttgtct tgtcatagac
     5821 tgtgatgttg tcatccacac tcaccgttac gaatggtgta tcaatcactc cgttttggta
     5881 aataccctca cggtgttctt gttttccttc cagcaattgg aaggttgtcc aagaaagcgg
     5941 tgctaggtga actggaatgg tcacacgcac ttgtcgagca atacgagctt ggcggaactt
     6001 atcttttggt aaatcgtatt caaaattagc tccgaggtct tcgattgtag cctctacagg
     6061 acgaccatcc aagtcctcca cacggtaact tggcaagata agagcagcca tctttttgta
     6121 gccttctgtt ggatgcaatt ccttgaaatc acaagtcgcc atatcaatca ctgtgctgac
     6181 agtatcgacc ttatcatgca agcctgtgtt aatgacagta aagagatagt cactttgagc
     6241 cttatccgta gcaattttac ccttccactc gttgagcaaa ttacttttaa caaagtttcc
     6301 tacttggttg accttggcaa aacgcgtttc catctcgcgg tgaacttcgt ccacgctaca
     6361 gccacagata ctatcatgtg gcgcattctg caaaagtgtt ttccaagcat aggtcaactg
     6421 gtccttgtgg ttgtgtccac cagtgataat agtcaagggt tctaccactt gctctaggag
     6481 gttgctattt tcttggaagg cttgttttag gtaaatgcgg gatgaagaag tgttggcaag
     6541 tgtgtaccag ccatctgttt cctgactggt caactcgcct gtaacagttg ataagtgttc
     6601 aggaagcgca ccttctacag cttgaacata ttcatcaaaa gaactatgaa caaagattac
     6661 atccgggaag agttcatttg ccacacgaat ggcttcgctc agatttttct gtacaggctg
     6721 gtggtcacag ccgttcatca tcaaccattg gttggtcgaa gcgtaggcac gcacatctga
     6781 caatttttgt ttccagaagg tcaaggcctc atctttgtca actgggattt cattcccatt
     6841 actgtaccag ttggcaaaga gaatacctaa aacacgacta ccatccacac cctgccagta
     6901 catttctgaa aactgggatg taaactgctc atcttcaagg acttggttgt caaatccaat
     6961 cggcttcaca ccacgaccaa aggccgccac gtgaatgcct gatttttgaa gaatttgagg
     7021 cgcttgtccc atatttccaa aggtatctgg aaagtagcca atctgggttg atttacccca
     7081 tttggcagct tcttgttgac caatcaaggt attgcggaca ttggcttcac tggagatcaa
     7141 gtagtcatcc tgcaagatgt aaaagggacc aattttaagt ttgccctcgt caatgtagcg
     7201 ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag tcatcaagga caatagtttg
     7261 tccatccaag tggaaactct tgaactcagg gtcattttca aagagatcaa agagattgtc
     7321 aaacaattcc accaactgca tacgatggct ttcaaaaggc aagtaccact cacggtccca
     7381 gtgactatgt gagataatat gtacaacaac attttccatg aagtaaaacc tcattctaaa
     7441 tttaaatttt aacgttttaa atgtaaactt gtgattctga taagaatcgg ttgagctaaa
     7501 gcgagcccct tagcgaatat ccaagtaatc caagaccaac tcacagaaca tcatgttagc
     7561 ccaggagaac cattcacgag agtagagggt cggatcatct acgtggaagc tttcgtgcat
     7621 gacacctgta ccaccatcgc aggcaaccag ctgatccagc aagaattttt tctctgcctt
     7681 atctcttgtt gtcaatcctt ggatagaaag ggcgattggc cagatatagc gatagaaggt
     7741 atgagaactt ccgagaccgc tagcgtattc tccttggtag aagtatggat tttcagagct
     7801 cagaatggta cggcgagtgg cttgatacac ttcatcatcg atcgaacagt agcccagata
     7861 gggcgcagct agtagacttg gtacatttgg atcatccatg atgctggcat ttcctaggcc
     7921 atctacttca aaagcgtaaa tcttttcgcc cttgctgttg gtggtgtaag cgtagttttc
     7981 gattccttct tggatttcat cctgaagacg cttggcatca gcaataacac tctggctatc
     8041 agctaggtct aattcgacaa agatttcttg cacataaccc aagactacta cggcaaacat
     8101 attagacgga atcaagtaac tatactgaca gcaatcgtca ctcggacgaa aagctgacca
     8161 agtcatacct gtcactgcaa agtcaggtcc aaagccatca tttaccaagg tgtcttcctt
     8221 acggtccgta tctcggacaa aacgataagg agagttcttg tggtcttgtt caactgtcca
     8281 tagatggaga atttccttag tcgctgcgac aaaagtttca tcaaactgac tagtctcgcc
     8341 agtctctttc cagaggagat aagccaactg caaaggatag caaagcgaat ccacctcata
     8401 cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca gtctcgtggt gccctttcca
     8461 gttctcctca atgttgaagg agttagcata gggatccttg agtaccaagg tcatctgacg
     8521 tttgaccaaa cctgcaatgg tctgacgcag gagggcatct cttttagcta catgaaggta
     8581 gggtctgagt tgggctgtcg aatctcgaag ccacatggca ggaatatccc cagtcaagac
     8641 aaaagttgaa ccatcttcta agatttcaac cgtattgtcc aaggtatcgg tgtagcaacg
     8701 ctcgaaaaca tccacccact ctggatggtc cttagcccgc tctgctactt catctagcca
     8761 ttctctaaca atttctttcg aataaatcat cgtgcaattt cctctttcaa acaagtttca
     8821 ttttcagtat atagcttttg agacagaaaa tacatacaca aatgatagtc atttttctac
     8881 aaaatagtta tttttgaaac tccttttcta aaggccctct ttttctgata taatgtagaa
     8941 aaacagctaa aggaaagact atgaaaccac tacttgaaac catcgatacc cgctttggta
     9001 ctgctagcaa gcatgccttc tctcggggaa ataccctgcc atacacaggc gtgccttttg
     9061 ggatgaatta ttttgtgccc cagaccagtg accaggatgg atcttggttt tttgatccgc
     9121 atctgcctat ctttcagggg attcgattaa ctcaccagcc cagtccttgg attggggact
     9181 actcttggct ccttctgaca cctgtcacca gtcaactggg gggagacagc ctctttcacc
     9241 gccagtcttc ttatgatata gataaagctt gcttccagcc tcactatttg aagctctttt
     9301 ccctgcgcta tcaaattgaa acccagctca caccgacttg ctatggtgct tctattcgtt
     9361 tgaagcaaaa gcaaggcaaa gccctctccc tctatcttca cgcagcagat gaactaacag
     9421 ttgagcaggt agataagcgg actctggctc tcagacaaga agggaaaaca gagaccaata
     9481 aaaatccact gatgctcttc actgccctgc aaatgaacac ggatattctt gctatcagcc
     9541 aagaagctgg agactggcga attgacttag caagtagtca aaccgagatg caactagcca
     9601 cttctttcat ctctccttct caagctctaa tcaatctacc tcaagaagat tttgatagct
     9661 gtaaaacaag tgcccaagcg gattgggaaa atctcctcca tcgttttgac attatagaga
     9721 caggagaggc tgaccgaacc ttctttgacc actgcctcta cagactcttc ctcttcccac
     9781 agacttttta tgaggttgat gagtcagggc aagccatcca catggatctg gctactggta
     9841 ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg ggataccttc cgcaccacct
     9901 tccccctctt tgcccttatc ataccggagc actatcaacg ctttttagaa ggtttcctca
     9961 atagctaccg cgatactggt ttccttccaa aatggctggc tccagatgaa cggggtatga
    10021 tgccaggtac acttttagac ggtattatcg cagatagcgc ctgcaaggac gtggcccctg
    10081 acctagaagg agaactcttc caagccatgc tcgaaacagc cagcaaagcc gaccctctcg
    10141 gaatcaatgg ccgccacgga ctagcccaat accaagaact aggttacctc tctaccgacc
    10201 accacgaaag tgttagccat actctagact atgcctatag tgacttttgt atcgctagct
    10261 gtgccaaaaa actagagaac atagaaatcg ctgaaaccta caaggctgct tcacaaaatt
    10321 accgccagct atttgacgct gagacaggtt acatgcgagc acgagacaat caaggaaact
    10381 ttcaccctga cttctctcct tatagttggg ggcgagacta cgctgaatgc tctgccattc
    10441 aagctacttt aggcgttctc cacgacatcc ctggcttaat ccaactgatg ggtggaaaag
    10501 aaacctttag caactatctt ttgaaagctt gtcaagatgc tcccctcttt gaaacaacag
    10561 gctatggtta cgaaattcac gaaatgagcg agatggctac tgctcctttt ggacaaatag
    10621 ccatttccaa ccagcctagt ttccacattc cttatctctt ccgctacagc gattaccctg
    10681 actacactgc ccttcttatc aagacgctcc gtcagaaagc ttttcaccca agttgggaag
    10741 cctatcctgg agatgaagac aatggtagtc tctctgcttg gtacatctgg tcagctctcg
    10801 gattttatcc aacctgtcca ggaaaaccaa gctatgacct cggaatccct ctctttgacc
    10861 atctacgagt ctacctcgct aaagaagata aatggctgga tatccatact aaacaaaacc
    10921 acaaccattt taactttgtc aaagaatgcc gactggacaa aacactcgta tcaactattc
    10981 aacaccaaga cctcttaaaa gctgaacaac taactttcac cctcagctgg ttaccaagtc
    11041 actaataacc aaaagaacct ttgcatcgcg caaaggttct tcttttatga aacttggact
    11101 tgaagttgat ccacaagctg accatccacc gttaggttca gatagaaatc caaggtttta
    11161 tctcctgcga aatacagggt tggtagatgc aaatcttttt tgctttcccc tgcttggaat
    11221 tgaaggactt gaatctcatc ctgataggcg acaccatgga cacctgtccc cggttgaatc
    11281 gaaatcttag cctctaaaga ggagctactt tcactacgtt caatcctaaa atgaagagtc
    11341 tctccctttg ccacagctag gcttttttct gcaaatgcta gcccctgaac agctactgtt
    11401 tgtgataacc tcggagtttt atagagtgaa atcttggtca acaaaggcaa aacctgtgat
    11461 tctgtaatga ctacacgtat cttctgtgct tcaacaactg ctcctcgtaa gagacgtttg
    11521 taaccaacag tatgacccga accaaactcc tgccagacac catccacctc tacttgcaca
    11581 tgaaaagcag cgattcgttg ccctagcttc aaatcttctc ttaactcaat tacatcaaaa
    11641 gttttaggag aacctaagtc gagttctaac tggatgggca aggctgcatc gtttgcccaa
    11701 gagctggtct caaggccgtc tgtcaaatgg cgacaagcaa agtctgcgga aagagctgga
    11761 ccagatacct cagctcccag agccaaatct tctttataga gctcattgcg ataggtcgca
    11821 aattcataaa gtcgttcaat atcctttgca tcaaagagcc cagcttgatt cggcggaata
    11881 ttaagcaaga gtggagttcc tcgccctact gagtgaaagt agatttcgac caactcctcg
    11941 agagacttag gatcctgatc ctcatggtag aaccagcctg gacggatgga aacatctgcc
    12001 tctccgattg aaaaaatcgt gcccgaggga tccccgtgct gaagatagtt cagctctgct
    12061 tctgttccta gtttatcagg attcaccttt tgccacagtg gatcacctgc ataccctcgt
    12121 tcattgccaa tccagcggat actggtgcct tctgttgaaa aaatcaagca agcgccctgc
    12181 aggtcacgaa tggtttcaaa ccatttttca aattcataat taaccttttg cgcgccctct
    12241 cctctggcac catccatcca aacctcagcg aacttaccag cattcccata gttaggattt
    12301 gataagattt ccttcaactg agccagataa taggcattgt agtccgcttc tcggtccaca
    12361 tgatagaggg gactatgggc atcccacggt gacaggtaga cccccatatc catatcaaac
    12421 tctgtggcag cttgggatac ttcaaggagc aagtcgccct ttcctctcct ccaaggactg
    12481 accttaaccg aataatctgt gtgagctgtc ggataaagga caaagccatc gtggtgcttg
    12541 accaccaaaa tcaacttttt gaagcccgtt tccttgagca cacgaaccca ctcacgcgca
    12601 tccaactgac tcgggttaaa gcgctcagga tcctcctgtc cagtccccca ttcttggtca
    12661 taaaaggtat taggaccgaa gtggataaaa gctgctagtt catctcccag ataagctagc
    12721 tgagtctgac ttggtaacgg tccatgcggt ttaattttct tcatcgttat atatctcctt
    12781 ctttagcaac ttctcttgtt cacaaggtta tccacaggtt gtggataata aaccatgatc
    12841 taaccgccag ctctttccaa ctccaactgg taggctcagg atttttctcc tcaatattcc
    12901 ctcttcaaac agctctgtca tatggttctg ctaacacctc tcagaatgct actctccata
    12961 attattctca ggcttctttc aggtcattgt gtgaagacca ttcactctcc agacttttcc
    13021 acagattgtg aataagtgat tattctaaac tatcacctcg tcgactataa caaaaaataa
    13081 aaagagggtc aataagataa ttatctcttc ctaaatactc agaaaagatt ctataatttg
    13141 tacaattgaa caagaaatgc ttctccaaaa tctatcttca aaagaatgtt gcatcttttt
    13201 atcctttcac acaaacattt ctctttatac agatccaagt gataaccaga tgataattgg
    13261 ac
//
LOCUS       DAVKJU010000057        13183 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVKJU010000057 DAVKJU010000000
VERSION     DAVKJU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13183
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.057"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..210
                     /locus_tag="VW191_001609"
     CDS             <1..210
                     /locus_tag="VW191_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEU9624353.1"
                     /translation="KGNSQDNGRMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYID
                     YYNNKRIKVKLKGLIPVQYRTKSFG"
     gene            complement(283..813)
                     /locus_tag="VW191_001610"
     CDS             complement(283..813)
                     /locus_tag="VW191_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000883388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HEU9624354.1"
                     /translation="MLDEKRKNYVEDKRQNFPSLPNWKMDLFSEEEKRNYFQTTCEIS
                     SKDSAINKYKMEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDVEGEWTALDDI
                     KNEAHMLKKLADKSYTTNLTDEFYIVDDYFLVQEQVDGLNFEEFIRETEHSLNIREKT
                     LDNIVNIVSDIHKLGI"
     gene            complement(859..1275)
                     /locus_tag="VW191_001611"
     CDS             complement(859..1275)
                     /locus_tag="VW191_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624355.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYYLSDKVPAQGWKIHISSQIK
                     DAVNIFKIVYKLSQLNNCSFKVVKNLEELKKINSPREMSPTANKFITLYPKSESEAKS
                     MICNLTNRLSEFKAPKILSDYQCGMHSLVHYRYGAF"
     gene            1824..2501
                     /locus_tag="VW191_001612"
     CDS             1824..2501
                     /locus_tag="VW191_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEU9624356.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            2488..3285
                     /locus_tag="VW191_001613"
     CDS             2488..3285
                     /locus_tag="VW191_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU9624357.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQVSTNPFRHI"
     gene            3302..4405
                     /locus_tag="VW191_001614"
     CDS             3302..4405
                     /locus_tag="VW191_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU9624358.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EEALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            4706..5725
                     /gene="galE"
                     /locus_tag="VW191_001615"
     CDS             4706..5725
                     /gene="galE"
                     /locus_tag="VW191_001615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU9624359.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTDTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            5727..6692
                     /locus_tag="VW191_001616"
     CDS             5727..6692
                     /locus_tag="VW191_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9624360.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(6743..6949)
                     /locus_tag="VW191_001617"
     CDS             complement(6743..6949)
                     /locus_tag="VW191_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU9624361.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            7001..7477
                     /locus_tag="VW191_001618"
     CDS             7001..7477
                     /locus_tag="VW191_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEU9624362.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            7486..8157
                     /locus_tag="VW191_001619"
     CDS             7486..8157
                     /locus_tag="VW191_001619"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU9624363.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            8287..8625
                     /locus_tag="VW191_001620"
     CDS             8287..8625
                     /locus_tag="VW191_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEU9624364.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIHIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            8628..9257
                     /locus_tag="VW191_001621"
     CDS             8628..9257
                     /locus_tag="VW191_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEU9624365.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            9497..10648
                     /locus_tag="VW191_001622"
     CDS             9497..10648
                     /locus_tag="VW191_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9624366.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGARLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            10966..11715
                     /gene="truA"
                     /locus_tag="VW191_001623"
     CDS             10966..11715
                     /gene="truA"
                     /locus_tag="VW191_001623"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU9624367.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQIDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            11705..12478
                     /locus_tag="VW191_001624"
     CDS             11705..12478
                     /locus_tag="VW191_001624"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU9624368.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            12456..12917
                     /locus_tag="VW191_001625"
     CDS             12456..12917
                     /locus_tag="VW191_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9624369.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         3793 a         2444 c         2893 g         4053 t
ORIGIN      
        1 aagggtaaca gccaagacaa cggtaggatg gaatctttct ttggcatttt aaaatcggaa
       61 atgttttatg gctatgagaa aacatttaaa tcacttaatc aattggaaca agccattata
      121 gactatattg attactacaa caataaacga attaaggtaa aactaaaagg acttatccct
      181 gtgcaataca gaactaaatc cttcggataa attaattgtc taacttttgg ggggcagtac
      241 attttggtat atataaaatt tgtaggagct atatctacaa ttttatattc ccagtttatg
      301 gatgtcactt actatattca caatgttatc cagtgttttt tctctaatat ttaaggagtg
      361 ttctgtttct cgaataaatt cttcaaagtt taacccgtca acttgttcct gaacaagaaa
      421 ataatcatcc acgatataaa attcatcagt taaattagta gtataacttt tatcggctaa
      481 tttttttagc atgtgagctt cattttttat atcatcaaga gctgtccatt ctccttcaac
      541 atcataattc acaaaaggtc ttgactgctt gatgattact ttttgcccgt ccgattttct
      601 aattgcccga taaacatttc ctttatttga tctcttaata attttttcca ttttgtattt
      661 atttattgca gagtctttac ttgaaatttc acatgtggtt tgaaaataat tccttttttc
      721 ttcttctgaa aataaatcca ttttccagtt agggagacta gggaaatttt gtctcttatc
      781 ttctacatag ttcttccttt tttcatctag caataaatag atgacttttt tatttttttc
      841 atcataagct tgtttttttt aaaaagcccc atatctataa tgaactagag aatgcattcc
      901 acattgatag tcagatagta tttttggagc cttaaattct gacagtctat tcgtaagatt
      961 acaaatcata ctcttagctt cagattctga cttaggatat agagttataa atttgttagc
     1021 agtagggctc atttccctag gggaattaat tttttttaat tcctctaaat ttttaacgac
     1081 tttaaagcta caattattta gttgggatag tttatacaca atcttaaaaa tatttacagc
     1141 gtcttttatt tgggagctta tgtgtatttt ccacccttgt gcaggcacct tatctgataa
     1201 ataatagtaa ttccaaatat caccctgttg gaaattttta tcacatttta aacattcttt
     1261 ctctaataaa tccataacaa actcctaatt attaaaatag gctagagaca cagcactagc
     1321 ctattccgta agtaaagcta cttattttat ataaaatata actagcgacc tgtaattaaa
     1381 caagtaaaag aacaatactt agggagacct gtgacaatga catcttcctc tggaagtagt
     1441 tctaattcaa acaatgtttg ttccatgact gtatcctcct gttatgataa attccagaaa
     1501 gcatagagca aattcgtggt tcgtctgact aaatttctta tctgaacacc accttcaaat
     1561 acaaatttta gtcaaacttt tattttttct aactttctaa ttatattcta ccatgtgttt
     1621 ttatatgtca agaattttct gaataaactg tttatttttt attttttgac atttttgcaa
     1681 attgtttaaa atctttcaat tggattcact tattacttta ggacattgaa acttctttcg
     1741 ccagaggtgc ttatattact gggaagttga agattgaaat gtggtataat aagaggtaat
     1801 agagttttgg aaagtgagag aagatgattt caaagagatt agaattggta gcttcctttg
     1861 tgtcacaggg ggctatttta ctagatgtgg gaagtgacca tgcttatctg cctatcgagt
     1921 tggttgagag aggccaaatc aaaagcgcta ttgcaggtga ggtggtggaa ggtccctatc
     1981 agtctgcggt taaaaatgtt gaggctcacg gcctaaagga gaaaatccaa gtccgtttag
     2041 ccaatggctt ggcagctttt gaagagactg accaagtgtc tgtcattacc attgctggca
     2101 tgggtggtcg tttgattgct aggattttag aagaaggttt ggggaagtta gctaatgtag
     2161 agcgtttgat cctccagccc aataatcgtg aagacgactt gcgtatctgg ctacaggatc
     2221 atggattcca gattgtagca gaaagcatct tagaagaagc tggaaagttt tatgagattt
     2281 tggtggtgga agcaggacaa atgaagctat cagccagtga tgttcgcttt ggtcccttct
     2341 tgtccaaaga agtcagtcca gtatttgtcc aaaaatggca aaaagaagct gagaagctag
     2401 agttcgccct cggacaaatc ccagaaaaaa atctggaaga acgtcaagtt ctagtagata
     2461 agattcaagc tatcaaggag gtgctccatg ttagcaagtg aagtgatcca agcttatgaa
     2521 gccttttgcc ctcaggaatt ttctatggag ggagacagcc gtggtctgca aattggcact
     2581 ttagacaagg gtatccaaag ggtcatggtt gccctcgata ttcgtgaaga gacggtggca
     2641 gaggccattg aaaagggtgt ggacttgatt atcgtcaagc acgcgcctat ctttcgtcct
     2701 atcaaggact tgcttgccag ccgtccgcaa aatcagattt acatcgacct gattaagcat
     2761 gatatcgcag tttatgtcag ccataccaat attgatatcg ttgaaaatgg gctcaatgac
     2821 tggttttgtc agatgctagg aatcgaggag acgacttatc tgcaggaaac aggtccagaa
     2881 cgtggaattg gacgtattgg gaatattcag cctcagacat tttgggaatt ggcccaacag
     2941 gtcaagcaag tctttgacct agatagcctt cgaatggtgc attatcaaga gaatgatttg
     3001 cagaagccta tttcaagagt agcaatttgt ggtggaagcg ggcagtcttt ctataaggat
     3061 gctttggcaa agggggcaga tgtctatatc actggcgaca tctactacca tactgctcag
     3121 gatatgctgt ctgatggctt gttagcattg gatccaggtc actatatcga agtgcttttt
     3181 gtggaaaaaa tcgcagcact ccttactcaa tggaaagaga aaaaaggctg ggaactagag
     3241 attttaccta gtcaagtatc gaccaatcct ttccgtcata tctagttaga aagtgaagac
     3301 aatgaaaaaa gttgccatta ttggagcagg aattgtggga gcaacagctg cctactacct
     3361 ctcgagagaa agtgacctag aggtgaccgt ttttgaccat gagcaaggtc aagccaccaa
     3421 ggccgcagca ggaattatca gtccttggtt ttccaaacgc cgtaataaag cctggtacaa
     3481 gatggcgcgc ttgggggctg atttttatgt ggatttatta gctgatttag agaaatcagg
     3541 acaaaaaatc gacttttacc agcgttcggg agtctttctc ttgaaaaagg atgaatccaa
     3601 gttggaagaa ctttatcaac tggccctcca gcgcagagaa gaatctccct tgatagggca
     3661 attagccatt ctggaccaag cttcagctaa tgaattattc cctggtttgc agggatttga
     3721 ccgcctgctc tatgcttctg gtggagcgag agtagatggc caactcttgg taactcgtct
     3781 gctagaagcc agtcatgtca agctggtcaa agaaaaagtg actctgacac tcttatcatc
     3841 aggctaccag attggcgaag aggtgtttga tcaggttatt ttggcaacgg gagcttggtt
     3901 gggggacatg ttagagcctt taggttatga agtggatgtc cgtcctcaaa aaggacaact
     3961 acgagattat cagcttgccc aagacatgga agcttaccct gttgtcatgc cagaagggga
     4021 gtgggatttg attccctttg caggtgggaa attatccttg ggcgctaccc atgaaaatga
     4081 catgggattt gatttgacgg tagatgaacc cttgctccaa caaatggagg aggaggcctt
     4141 gcctcattac ccagttttag ctagagcgac ttcaagggct gagcgtgtgg gaatccgtgc
     4201 ctataccagt gatttctctc ccttctttgg gcaggtgcca gaattggcag gtgtctatgc
     4261 agccagtgga ctaggttcat caggcctcac aactggtcct atcattggtt accatctagc
     4321 ccaactgatc caagacaagg agttgacctt ggaccctcta aattacccaa ttgaaaacta
     4381 tgtcaaacga gtaaaaagcg aataatactc aatgaaaatc aaagaacaaa ctaggtaact
     4441 agccgcgggt tactcaaagc actgctttga ggttgtggat agaactgacg aagtcagctc
     4501 aaaacactgt tttgaggttg tagatagaac tgacgaagtc agtaaccata cccatggcaa
     4561 ggcgacgttg acgtggtttg aattggattt tcgaagagta taagcatttt actgaaattt
     4621 tatcaggtag tttaggatgg caaatgacat tccctgtcaa aaatggtaaa ataagaaaaa
     4681 ataatctgag aatcgaggaa aaaagatgca agaaaagatt ttggtaactg gtggtgccgg
     4741 ttttattgga acccacactg ttattgaatt gatccaagca ggccatcaag ttgttgtggt
     4801 ggataatctt gtcaatagca atcgtaagag tttagaagtt gttgaaagaa tcacaggagt
     4861 tgagattcct ttctatgagg cagatattcg tgacacagat accctcagag atattttcaa
     4921 gcaggaagaa ccgactggtg tcattcactt tgctggtttg aaggctgttg gcgaatcaac
     4981 acgtatccct cttgcctact atgacaacaa tatcgctgga actgtcagcc ttttgaaagc
     5041 aatggaagaa aacaactgta aaaacatcat cttcagttct tctgcgacag tttacgggga
     5101 tccgcacaca gtgcccatct tggaagattt cccactttca gtgaccaacc catacggtcg
     5161 tactaagctc atgctagagg aaattttgac tgatatttac aaagcagact cagaatggaa
     5221 tgttgtcttg cttcgttact ttaacccaat cggagcccat gagagtggtg atttgggaga
     5281 aaatccaaac ggtattccaa acaatctctt gccatatgtg actcaagtag ccgttggaaa
     5341 attagagcaa gtgcaagtgt ttggagacga ttacgatacg gaagatggaa caggtgttcg
     5401 tgactatatc cacgttgtcg atttggctaa gggtcacgtt gcagctttga aaaaaatcca
     5461 aaaaggttca ggactaaacg tttataacct tggaactggt aaaggttact cagttcttga
     5521 aattatccaa aacatggaaa aagcggtggg acgtcctatt ccttaccgca tcgtagaacg
     5581 tcgcccaggt gatatcgctg cctgctactc agacccagca aaagctaaag cagaactcgg
     5641 ttgggaagca gaactcgaca tcacccaaat gtgtgaagac gcatggcgtt ggcaaagcaa
     5701 gcatccaaat ggatttgaag actaagatga tgatttcaat catcgtccct tgtttaaacg
     5761 aagaggaagt acttcctctt ttttatcagg ctctggaagc tttacttcca gatttggaaa
     5821 cagaaatcga gtatgtcttt gtcgatgatg gatcaagtga tgggaccttg gaactcttaa
     5881 aggcctatcg ggagcaaaat ccggcagtcc attatatttc tttctctcga aattttggca
     5941 aagaagcagc cctttatgca ggcttgcaat atgcgacagg agatttggtg gtggtgatgg
     6001 atgcagacct ccaagatcct cctagtatgt tgtttgagat gaaaaatgta ctagacaaaa
     6061 atgtagactt ggactgcgtt gggacacgga gaactagtcg ggagggagaa cccttctttc
     6121 gcagtttctg tgctgttctc ttttatcgcc tcatgcaaaa aatcagccca gtagctctgc
     6181 cgtcgggtgt ccgtgatttt cgtatgatga gaaggtctgt agtcgatgcc attttaagct
     6241 tgaccgagtc caatcgtttt tcaaaggggc tcttcgcctg ggtgggtttc aaaacctact
     6301 atttggacta tccaaatgtc gaaaggcagg ctggcaagac aagttggagt tttaggcaac
     6361 tctttttcta ctccattgaa gggattatta atttttcaga ttttcccttg attatagcct
     6421 ttgtagctgg tctcctatct tgttttcttt ctctgctgat gacctttttt gttgtggttc
     6481 ggaccctcat tttgggcaat ccgacatctg gttggacctc tctgatggct gttattctct
     6541 ttcttggagg cattcaactc ttgaccattg ggattctcgg caagtatatc agtaagattt
     6601 atttagagac taaaaaaaga ccactttatc ttatcaaaga aaaaagtgac cttcctgatt
     6661 ttacagaaaa aaataaagtg aaaagactat aattttacat ggaaatgtgc taaactagag
     6721 ggagtggatt atcgcccttg atttaggctt cctggtttcc aatcagggcg cgagttgggc
     6781 aatttttaac agcctctaag acatcctgac taggagaaat ttctttttcc agttggtcag
     6841 ggtcatcgta aaaacgcacg attccattat cgtggtaatc aaataaatca gaataagttt
     6901 ggcaaagccc acaggcgata catcgttcag gtataagtgt gattttcatg ttcatattta
     6961 tattgtaata agaaaagtaa aaaaaaacaa ggagtaaggt atggcaaaag aaccgtggca
     7021 agaagatatc tatgatcaag aagaatcaag agcagagcgt cggcatcgaa accacggagg
     7081 ggctgatagg atggctaatc gtattttgac gatcctagct agtattttct ttgtaattgt
     7141 ggtggtgatg gtcatcgttc tcatctatct atcatcgggg ggaagtaatc gcacagcagc
     7201 cttaaaagac tttcatgatt ctgatgcaag tgtagtacaa atctcatctt caagcagttc
     7261 tcagcctgag cagagttcag agccagaatc tacttctagt agttcagaag aagctgctaa
     7321 tcctgaagga acgattaaag ttctcgcagg agaaggggaa gcagctattg ccgctcgtgc
     7381 aggaatctcc attgctcagt tagaggcctt gaatcctggg cacatggcta caggatcttg
     7441 gtttgctaat ccaggtgatg ttataaaaat aaaataggag ccgatatgaa aacaattcaa
     7501 attgctattg atggtcctgc ttccagcggt aagagtacgg tcgcaaagat tattgctaag
     7561 gattttggat tcacctacct tgatacagga gctatgtatc gtgcagcgac ctatatggct
     7621 cttaagaacc aattaggagt tgaagaagtc gaagcccttc tagccttgtt ggaccagcat
     7681 ccaatcagct ttggacgttc agaaactgga gaccagcttg tttttgtagg agatgtggat
     7741 attacccatc ctatccgtga aaatgaagtg accaatcatg tttctgctat tgcagcaatt
     7801 cctgaagtgc gtgagaaact ggtttctctc caacaagaaa ttgcccagca aggcgggatt
     7861 gtcatggatg gtcgcgatat tggaactgtt gtattgccac aagcagaatt gaaaattttc
     7921 ctagtagctt ctgttgatga gagagcagag cgtcgttaca aggaaaatat tgccaaggga
     7981 attgaaacag accttgaaac cctaaaaaag gaaattgctg cgcgtgacta caaggatagt
     8041 catcgtgaga cttctcctct caaacaagca gaggatgctg tctaccttga tacaactggt
     8101 ttgaacattc aagaagtagt tgaaaaaatc aaagcagaag ctgaaaaaag aatgtaaatg
     8161 tagaaaagcc gatagattga tatcggtttt ttctaaattt gtcaaaatgc tgattttagg
     8221 gtataatagt tgctgttcga gaaattttga ttttcaaaga atagtgaatg atgataagga
     8281 gaaatcatga acaatttacc aaattgtcca aaatgtaact cagagtatgt ctacgaagac
     8341 ggtgccctac tggtttgccc agagtgtgct catgagtgga atcctgctga agttgcagaa
     8401 gtagaagaag gtcttgtcgc tatcgatgcc aacggaaata aattggctga tggtgataca
     8461 gtaactctca tcaaggactt gaaagtaaaa ggtgcgccaa aagatttgaa acaagggacg
     8521 cgcgtgaaaa atatccacat cgtagaaggc gaccacaata tcgactgtaa gattgatggt
     8581 tttggtgcca tgaaacttaa atcagagttt gtgaggaaga tttaagcatg tcaaaacact
     8641 ataaacttgt attttatagc cgtatcttct tgtttctagc ggcttttacg ggagtttatc
     8701 ttgaaattac taagcatggt ggttttggga tgcttctcta ttacacggtt ctgtccaacc
     8761 tcttggtaac tatttttacc ctttatcttc taaaggttat gagccgtgta ggtgaaaatt
     8821 ggcaaagacc aagtctcttg cgcttaaaag gtggggtcac catgagtatc atgattacct
     8881 gtgtgattta ccatttcctc ttggcgccca ttgcgactaa tttctatacc ctagaaaatt
     8941 tcctttgcca ctatatcgtt cccatctggt ttttagcgga taccctcttt tttgacaaac
     9001 agggtcaata caagatttgg gatccagcag tgtggaccat tttacccttt ctgtatatga
     9061 tgtttgctct ttttaatggc ttggttctaa aactcaatat tccaaatgcc aaggataatc
     9121 ctttccctta cttctttttg aatgtgaaca agggttggaa tgttgtgttt aagtggtgtc
     9181 tgattatctt tgttgcctat atggtagcag gatttatttt ctactttatc aagcaaatca
     9241 agagaaagtc atcctaatac tcttcgaaaa tctcttcaaa ccacgtcagt ttccatctgc
     9301 aacctcaaaa cactgttttg aacaacctgc ggctagcttc ctagtttgct ctttgatttt
     9361 cattgagtat aagatacaag ggattcattt tcgaatttaa gaaggagtct acaaaccaat
     9421 gaggcttctt cttttcttgc ttatttgata actatcaaaa aagtagaaga ttaagagaaa
     9481 tatagaaaga gatttcatga aacaattttt agaacgggcc agcattttgg ctctctccct
     9541 cgttttgatt acctcctttt ccatttcgag tgccctacca gccatgtttg actattatca
     9601 gggttattct aaggaacaaa ttgagctctt ggtgagcttg ccttcctttg gaatcatgat
     9661 gatgttactg ctaaatggtt tcttagaaaa aatatttcct gagcgcttac agattagttt
     9721 gggcttgctg attttatcat tgagcggtac agctcccttc tggtaccaag cctatccctt
     9781 tgtctttgga gcacggcttc tctttggttt gggtcttggg atgatcaatg ccaaggccat
     9841 ttctattatc agtgaacgct accaaggaaa aaggcgaatt cagatgttag ggctacgcgc
     9901 ttctgcagag gtcgttggag cttctctcat taccttggcc gtcggtcagt tgttggcctt
     9961 tggttggaca gctatctttc tagcctatag tgctggattt ttggtgctgc ccctttatct
    10021 gctctttgtc ccttatggaa aatcaaagaa agaagtcaag aaaagagcga aggaagcaag
    10081 tcgtttaact cgagaaatga aaggcttgat ttttacctta gctatcgaag cggcagttgt
    10141 agtttgtacc aatacagcta ttaccatccg tattccaagt ttgatggtgg aaagaggatt
    10201 gggggatgcc cagttatcta gttttgttct tagtatcatg cagttgatcg ggattgtggc
    10261 tggggtgagt ttttctttct tgatttctat ctttaaagag aaactgctcc tctggtctgg
    10321 tattaccttt ggcttggggc aaattgtgat tgccttgtct tcatccttgt gggtggtagt
    10381 agcaggaagt gttctggctg gatttgccta tagtgtagtc ttgacgacgg tctttcaact
    10441 tgtctctgaa cgaattccag ctaaactcct caatcaagca acttcatttg ctgtattagg
    10501 ctgtagtttc ggagccttta cgaccccatt tgttctaggt gcaattggct tactaactca
    10561 caatgggatg ttggtcttta gtatcttagg aggttggttg attgtaatct ctatctttgt
    10621 catgtaccta cttcagaaga gagcttagga ttgattccta agtttttctt ttgatactca
    10681 atgaaaatca aagagcaaac tatagttgat tgagtttgga atagtacgct gtagctgcta
    10741 aaatatttct agaaattaat ttgactttcc taatagagtt attcatatct tatttcaatc
    10801 tactatagaa agctagccgc aggttgctca aaatagtgtt ttgaggttgc agatggaaac
    10861 tgacgtggtt tgaagagatt ttcgaagagt atgagatttt tatacccata caatatttgt
    10921 tttaggtcct tgtttacaga ctaatttctt gataaaatag aggttatgac gagatataaa
    10981 gcaactattt cctatgatgg ttatgccttt gctggctttc agcgccagtc tcatgcgcgt
    11041 agcgttcagg aagaaattga aaaaaccttg accagattaa ataaagggca agccattact
    11101 gttcacggtg ctggtaggac agatagtggg gttcatgccc tgggacaggt cattcatttt
    11161 gacctgcctt atcagattga tgaggagaaa ctccgttttg ccttggatac ccagtctcct
    11221 gaagatattg atgtgatttc gattgagctt gtggcagatg attttcattg ccgttatgcc
    11281 aagcatagca agacctatga gtttattgtg gatagaggac gtcccaaaaa tccgatgcgc
    11341 cgtcactatg ccacccactt tccctaccca ctcgatgtgg aacgaatgca gattgcaatc
    11401 aaaaagctag agggaaccca tgattttacc ggttttacag cctctgggac tagtgtagag
    11461 gataaggttc gcaccatcac agaagctagt ttaatagtcg atgagacagg acaatttttg
    11521 acctttacct tttccggaaa tggtttcttg tataaacaga ttcgcaatat ggtggggact
    11581 cttctcaaaa tcggtaacaa ccgcatgcca gtagagcaga ttgatctcat cttggagaag
    11641 aaggacaggc aacttgcagg tcccactgca gcaccaaatg gtttgtattt aaaggagatt
    11701 cgttatgaag aataatcgta ttttagcact ttctggaaat gatattttta gtggtggtgg
    11761 actgtcagct gatttggcta cctatacctt gaacggcttg catgggtttg tagcagtgac
    11821 ttgtttgaca gccttgacag aaaaaggatt tgaagtcttt ccaactgatg ataccatttt
    11881 tcaacatgaa ttagatagct tgcgtgatgt ggaatttggg ggaattaaga ttggtcttct
    11941 ccctactgtc agtgtggctg agaaggcctt ggactttatc aaacaacgcc caggagtacc
    12001 tgtggtgttg gatcctgtct tggtctgcaa ggaaacgcat gatgtagctg tcagtgagct
    12061 ctgccaagag ttgattcgct ttttccctta tgtcagtgtg attacgccta atctcccaga
    12121 agcagaatta ttatccggtc aggaaattaa aaccttggaa gacatgaaaa ctgcagcgca
    12181 gaaattgcat gatttaggag cgccagcagt cattatcaag ggaggcaatc gtcttagtca
    12241 ggacaaggct gtggatgtct tttatgatgg acagaccttt actatcctag aaaatccagt
    12301 tatccaaggc caaaatgctg gtgcaggttg tacctttgcc tctagcattg ccagtcactt
    12361 gattaaaggt gataaacttt tgccagcagt agaaagctct aaggctttcg tttatcgtgc
    12421 tattgcacaa gcagatcagt atggagtaag acaatatgaa gcaaacaaaa acaactaaaa
    12481 tcgcccttgt atccctatta accgcccttt ctgtggttct aggttatttc ttaaaaatcc
    12541 caacacctac aggaattcta actcttttag atgctggtgt cttctttgcg gccttttact
    12601 ttggtagtcg tgaaggagcg gtagtcggag gactagcaag tttcttgatt gacctcctat
    12661 caggctaccc tcagtggatg ttctttagct tggtcaacca tggcttgcag ggatttttcg
    12721 caggatttaa aggaaaaagt cagtggttag gccttatttt agcaactatt gccatggtag
    12781 gaggctacgc cttgggttct actttgatga atggctgggc agcagccctc ccagaaattc
    12841 taccaaattt catgcaaaat atggtaggga tgattgtagg atttattctt agtcaaagta
    12901 tcaagaagat taagtaatac tcttcgaaaa tctcttcaaa ccacgtcagt ttccatctgc
    12961 aacctcaaaa cactgttttg agcagcctgc ggctagcttt ctagtctgat tttcattgag
    13021 cataaagagg ttgagtaaaa agtctttcaa ataagaaaaa cgcatagtat caggtgttga
    13081 atgaactgcc ccccaaaagt tagacagaaa aaatctaact tttggggtgt tttattatga
    13141 aactaactta tgatgataaa gttcagatct atgaacttag aaa
//
LOCUS       DAVKJU010000058        11896 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVKJU010000058 DAVKJU010000000
VERSION     DAVKJU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11896
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.058"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            165..818
                     /locus_tag="VW191_001626"
     CDS             165..818
                     /locus_tag="VW191_001626"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU9624370.1"
                     /translation="MEHSSWHALIKAQLPEGYFRKINQFMEQVYSKGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            828..1292
                     /locus_tag="VW191_001627"
     CDS             828..1292
                     /locus_tag="VW191_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEU9624371.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTSDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1305..2573
                     /locus_tag="VW191_001628"
     CDS             1305..2573
                     /locus_tag="VW191_001628"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU9624372.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGSETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKVPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2587..2847
                     /locus_tag="VW191_001629"
     CDS             2587..2847
                     /locus_tag="VW191_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624373.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRICACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2858..4219
                     /gene="pdrM"
                     /locus_tag="VW191_001630"
     CDS             2858..4219
                     /gene="pdrM"
                     /locus_tag="VW191_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEU9624374.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAVFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASIYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     FKC"
     gene            4373..5341
                     /locus_tag="VW191_001631"
     CDS             4373..5341
                     /locus_tag="VW191_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU9624375.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5357..6349
                     /locus_tag="VW191_001632"
     CDS             5357..6349
                     /locus_tag="VW191_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU9624376.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNEVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6567..7610
                     /locus_tag="VW191_001633"
     CDS             6567..7610
                     /locus_tag="VW191_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEU9624377.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7674..9359
                     /gene="lpdA"
                     /locus_tag="VW191_001634"
     CDS             7674..9359
                     /gene="lpdA"
                     /locus_tag="VW191_001634"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU9624378.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASSVPVASTSNDDDKS
                     DDAFDIVVIGGGPAGYVAAIKAAQFGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDCIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9423..10412
                     /locus_tag="VW191_001635"
     CDS             9423..10412
                     /locus_tag="VW191_001635"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEU9624379.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEITEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10477..11547)
                     /gene="xerS"
                     /locus_tag="VW191_001636"
     CDS             complement(10477..11547)
                     /gene="xerS"
                     /locus_tag="VW191_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEU9624380.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3536 a         2214 c         2655 g         3491 t
ORIGIN      
        1 gatttaaaaa gtaattataa aaaatagcca gtctttataa actgtattca cgtattcaaa
       61 tcgtatatat agtcgattta ttaaatcttt agataaactg taaaaagtgg tttccatagc
      121 cactttttgc tataatagag gcagtaaaaa tgaaggagtc agctatggaa cactcgtctt
      181 ggcatgcttt gattaaggcg caattacctg agggttattt caggaaaatc aatcagttta
      241 tggagcaggt ctattctaag gggactattt atccacccaa ggaaaaggtt tttcaggctc
      301 tcttgacaac actgcttgaa gaagttaagg tggtaattct agggcaagac ccctatcacg
      361 gaccaggtca agcgcagggc ttgagttttt ccgtacctga ctctatccca gctccaccat
      421 ccttgcaaaa tatcttgaaa gaattgtcag atgatatcgg agttaagaaa tctcatgatt
      481 tgacagcttg ggctgagcaa ggagtcttgc ttcttaatgc ttgtttgact gttcctgctg
      541 gacaggctaa tggtcatgct ggtcaaatct gggagccttt tactgatgct gtgattcagg
      601 tggtcaatca tctagataga ccagtcgttt ttgtactctg gggagcttat gcacgtaaga
      661 agaaggcctt agttaccaat cctcatcact tgattatcga atcagcccat ccaagtcctt
      721 tgtcggttta tagaggattt tggggttcca agcccttttc taaggccaat gcattcttaa
      781 aagagacagg acaagagcca atcgattggc ttagataagg agaaaatatg cctcagttag
      841 cgacgatttg ctacattgat aatgggaaag aactgctcat gctccatcgt aataagaaac
      901 ccaatgatgt ccatgaaggg aaatggattg gtgtgggtgg taagctagag agaggagaga
      961 cgccccagga atgcgcggcg cgtgaaatcc ttgaagaaac agggctcaaa gccaagccag
     1021 ttctaaaagg tgtcatcact tttcctgaat ttacatcaga tttagactgg tacacctatg
     1081 tttttaaggt gacggagttt gagggcgact tgattgactg caatgagggg acgctagaat
     1141 gggttcccta tgatgaggtt ttgagcaagc cgacttggga aggtgaccac acctttgttg
     1201 agtggctttt agaggataaa cccttctttt cagccaagtt tgtttatgat ggggataaat
     1261 tgttggatac ccaagttgat ttctatgaat aaaggagaaa gcagatgcta ctaatcaaaa
     1321 atggtcgtgt aatggatccc aagtctggtt tggatcaagt ttgtgatgtc ttagttcaag
     1381 atgggaaaat tatcaaaatt gcgcctgaga tcacggaaga aggatcagaa acgattgatg
     1441 ctactggtca tgtggttgct cctggtttgg tcgatattca tgtgcatttc cgtgaacctg
     1501 gtcaaacaca taaagaagac attcatactg gtgccctagc agccgctgca ggtggtttta
     1561 ctactgtcgt catgatggct aataccagtc caaccatttc agacgtggag actttgcaag
     1621 cagttctcca gtcagctgcc aaagagaaga ttaatgtcaa gacagttgcg accattacta
     1681 aaaactttaa tggtaaaaac ttgactgact ttaaggcact tttagaagct ggtgcggttg
     1741 gtttctctga tgacggtatt ccgcttgaga gcagtaagat tgtcaaggaa gccatggagg
     1801 aagccaaaaa actcaatacc tttatcagcc ttcatgagga agatccaggt ttgaacggtg
     1861 ttcttggctt taatgaaaat attgctagag aacatttcca tatctgcggt gctactgggg
     1921 tggctgagta cgctatgatg gcgcgtgatg tcatgattgc ctatgcaact aaagcccatg
     1981 ttcacatcca gcatttgtct aaggaagaaa gtgttaaagt agtggagttt gctcaggggt
     2041 taggtgcaga agtcacagca gaagtagcgc cacagcattt ctctaagaca gaagcacttc
     2101 ttttaacaca aggtagcaat gctaagatga atccaccgct tcgtttggaa tcagaccgtc
     2161 gtgccgttat cgaaggtctc aaatcaggtg tcatcacagt tattgcgact gaccacgcgc
     2221 ctcatcatgt agatgaaaaa aatgttgagg atattaccaa agtaccatct ggtatgactg
     2281 gcttagaaac atccctgtct ctcggcttga cctatttagt agaagctggt gagttgagct
     2341 tgatggaatt acttgaaaaa atgacataca acccagccaa gctttacaac tttgaagcag
     2401 gttacttggc tgagaatggt ccagcagata tcactatttt tgatgccaag gctgaccgcc
     2461 ttgtggactc ccattttgct tccaaagcag ctaattcacc attcatcggt gaaaccttaa
     2521 aagggcaggt taaatatacc atctgtaagg gacaaatcgt ctatcaagct tgataggagt
     2581 tagaatatga attattttcg aaaacgtagg gagagacaag cgaaatctaa tagtggaatt
     2641 tactgtcccg cagctaatcg ttccaaaatt caatatgaaa ctaaagaaaa agctgaccat
     2701 gcggttcaat atgataacgg aggtttagtt agaagctatt attgtcgtat atgtgcgtgt
     2761 tggcatacta cttcaaaatc taataaacct ccgctaagtt tgaaaaacta tggtctgaaa
     2821 ctctttggtt tagataaatc agaatagaaa gagaagtatg tataagacaa agtgtttacg
     2881 agagaagtta gtattatttt taaaaatttt cttcccaatc ctgatctacc aatttgccaa
     2941 ttattctgcc tcttttgttg atactgcaat gacaggtcaa tacaacacta tggacttggc
     3001 tggtgtatct atggcaacca gtatctggaa tcctttcttt acatttctaa cagggattgt
     3061 gtcagccttg gtgcctatca ttggtcacca tcttggtcga ggcaaaaagg aagaagttgc
     3121 gtctgatttt taccaattta tttatttggc cttgggccta tctgtggtct tgctggggat
     3181 ggtacttttc ttggcaccaa caatcttgaa tcatattggg ttagaagcag cagtagcggc
     3241 agtagcggtt cgctatcttt ggtttttatc tatcgggatt atccccttgt tgctctttag
     3301 cgtcattcgt tccttactgg attcgctggg cttgaccaaa ctgtccatgt acctcatgct
     3361 tttgttactc cctctcaata gcggatttaa ctatctcttg atttacggtg cctttggtgt
     3421 tccagaactg ggaggggctg gtgctggttt aggaacatcc ttggcctact gggtcttgct
     3481 tgggatttct gttctggttt tatttaaaca ggagaagctc aaagccttac accttgagaa
     3541 acgaattcca cttaatatgg ataaaattaa ggaaggagtt cgtttaggtc tgcctattgg
     3601 gggaactgtc ttcgcggaag tggctgtctt ttcagtggtt ggcttgatta tggctaagtt
     3661 ttcgtccttg attatagcta gtcaccagtc agctatgaac ttttcaagtc ttatgtacgc
     3721 ctttcctatg agtatctcat cggctatggc tattgtcgtt tcctatgaag tgggagccaa
     3781 gcgatttgat gatgcgaaaa cctatattgg tctaggaaga tggactgccc tcatttttgc
     3841 ggccttcacc ttaaccttcc tttacatttt taggggaaat gtggccagta tttatggtaa
     3901 cgacccaaaa tttatcgatt tgacagcgcg ttttttaact tatagtcttt tcttccagtt
     3961 agcagatacc tttgcggcgc cgcttcaggg aattttgcgg gggtataagg atacagttat
     4021 tcctttttac cttggtttgc ttggttattg gggcgtagca atccctgtgg ctacgctatt
     4081 tgattcccta acagattttg gagcttattc ttactggatc ggcttgatta ttagtttgat
     4141 tgtgagtggg gcgctctacc gttggcgttt aactgtgatt atgaagagat ttgaatcttt
     4201 agcaaaattc aaatgctaaa aagtttgtga aaaaatattc ttgacaagaa aatcccttgt
     4261 tttcacgggg ttttcttttt tattttgtga aatttctttt agaaaaattt tgacagtatt
     4321 tgccaaaagc ggtaaaatag taaggtaaga agaaagttag aaaggcaaga agatgtcaac
     4381 tttagataaa aatcttttgc tagaaatgtt ccgtaagatg gaagaaatcc gtcgcatgga
     4441 cttaaaaatt gcacaattag taaagaaagg gaaagtgcca ggaatgacgc acttttctgt
     4501 tggtgaagag gcagctaacg tgggggctat gttagctctc aatccagatg atctgattac
     4561 ctcaaaccac cgtggacacg ggcaagctat tgctaaaggg attgacctca acggaatgat
     4621 ggctgaaatc cttggtaaat acactggaac ctgtaaaggg aaaggtggat ctatgcatat
     4681 cgctgacctt gatgctggga accttggtgc caatggtatc gtaggtggtg gtatggggat
     4741 cgctgtcggt gcagccctca gtcagcaaat gcaaaatacc ggtaaaatcg ttgtctgctt
     4801 ctttggagat ggtgcgacca atgaaggtgt tttccacgaa gcagtgaaca tggcttctat
     4861 ctggaacctg ccagtcattt tctattgcat taacaacggt tacggtatct ctgcggatat
     4921 caagaaaatg accaatatag aacatatcca tcaacgtagc gccgcttatg gaattcctgg
     4981 aatgttcatc gaagacggta acaatgtcat cgatgtctat gaaggatttc agaaagctgt
     5041 ggaccatgtt cgcagtggca atggtccagt cttgattgaa agtgtaactt atcgctggct
     5101 cggtcactca tcatctgacc ctggtaaata tcgtacgcgt gaagaagtgg aattgtggaa
     5161 acaaaaagat ccaatcgaaa acctccgcaa ttaccttatt gaaaataaca ttgcaagtgc
     5221 cgaagaattg gaagaaatcc aagcgcaagt aaaggaagca gtagaagctt ctgttaaatt
     5281 tgcagaggaa agcccattcc caccgcttga atcagccttt gaagatattt acgcagacta
     5341 aggagaaaga gataaaatgg aaacaaaaac aatgtccttc cgtgacacca ttatccttgc
     5401 tatgtctgag gaaatgcgtc gcgatgaaaa tgtgttcttg atgggagaag acgtcggtgt
     5461 cttcggagga gacttcggta cttctgttgg aatgcttgaa gaatttggtc cagaacgtgt
     5521 ccgtgactgt ccgatttctg aagctgccat ctctggagca gcagcaggag cagccatgac
     5581 aggacttcgt ccaatcgtcg atatgacctt catggacttc tctgttattg ccatggacaa
     5641 tatcgtcaac caagctgcta aaacacgtta catgtttggt ggtaaaggtc aggttccaat
     5701 gactgttcgt tgtgcagctg gtaacgaagt tggctctgcc gcccagcact cacaatctct
     5761 agagtcttgg tttactcaca ttccaggact taaggttgtg gcacctggaa cacctgcgga
     5821 catgaaaggc ctgcttaagt cttctatccg tgataacaac ccagttatta tacttgagta
     5881 caagtcagaa tttaaccaaa aaggggaagt gccagttgat ccagactaca caattccact
     5941 tggggttggt gaaatcaaac gccaaggaac ggatgtaaca gttgttactt atggaaaaat
     6001 gcttcgccgt gtggttcaag ctgctgaaga attagcagaa gaaggaattt cagttgaaat
     6061 tgttgaccca cgtacccttg ttccgcttga taaggatatc atcataaact cagtgaagaa
     6121 gactggtaag gttgttctgg tcaacgatgc ccacaaaaca agtggctata tcggtgaaat
     6181 ttcagctatt atttcagaat cagaagcatt tgactatcta gatgcaccaa ttcgccgttg
     6241 tgcaggagaa gatgtgccga tgccttatgc acaaaaccta gaaaatgcaa tgattccaac
     6301 agttgaaagt atcaaagatg caatccgaaa aacttataac aaagaataga gttacataag
     6361 ataagttatg taaataaaat aaagctgacg agaaactttg tatcttttag gtgcagagct
     6421 cttcgtcatt aatatcttag attagagcac gggctaaaag ctcggaaaaa agataaatct
     6481 ccttggcttc atcgaagcca tcgtcgattt cctatttttc agtcgctttt gacgcccttt
     6541 gtatcataaa tagaattgga gaggtcatgg ctgatgacaa gctaagagcg actcctgcag
     6601 ctagaaagtt agcggatgat ttagggataa acctctacga cgtttctggc tcaggtgcaa
     6661 acggtcgtgt ccacaaagaa gacgtggaaa cttataaaga cacaaacgtg gttcgcattt
     6721 cgccacttgc aaaacgaatt gccctcgaac ataacattgc ttggcaggaa atccaaggaa
     6781 ccggtcatcg tggtaaaatc atgaagaagg atgttttggc cctgcttcct gaaaatatcg
     6841 aaaacgatag catcaaatct cctgctcaga ttgaaaaagt ggaagaagtc cctgataacg
     6901 taacaccata tggtgaaatc gagcgtattc caatgacacc aatgcgtaag gttattgccc
     6961 aacgcatggt tgaatcttac ttgactgcgc caaccttcac cctcaactat gaagttgata
     7021 tgactgaaat gttggctctt cgtaagaagg ttcttgagcc aatcatggaa gcaactggga
     7081 agaagactac tgtaacagac cttctttcgc ttgcagttgt taagactctt atgaaacacc
     7141 catacatcaa cgcttcattg acagaagatg gcaagactat tatcactcac aactatgtca
     7201 atcttgccat ggcagttggg atggataatg gattgatgac acctgttgtt tacaatgctg
     7261 agaaaatgag tctttcagaa ctggttgtag cctttaagga tgttattggc cgtaccttgg
     7321 atggtaaatt ggctccaagt gagttacaaa attcaacatt cacaatcagt aatttgggaa
     7381 tgtttggtgt tcagtccttt ggtccgatta ttaaccaacc caactcagct atccttggtg
     7441 tcagttcgac aatcgagaag ccagttgtcg tcaatggtga aattgtgatt cgcccaatca
     7501 tgagtttagg attaaccatt gaccaccgtg tcgtagatgg tatggctggt gctaagttta
     7561 tgaaggactt gaaagagttg attgaaacgc caatctcaat gttaatttaa gagcaagagt
     7621 attcattttt aagatataga taaaggaagg aagacatggc cttagaagta attatgccaa
     7681 aagccggcgt ggatatgaca gaaggacaaa tcgtccaatg gaataaaaaa gtcggagaat
     7741 ttgtaaaaga aggagaaatc cttttggaaa tcatgactga taaagtcagc atggaattgg
     7801 aagccgaaga agatgggtac ttgattgcca ttctcaaagg agatggtgaa actgtccctg
     7861 taacggaagt tatcggttac cttggtgaag aaagggaaaa catcccaaca gctggagcag
     7921 catcgccaga agctagctct gtacctgtag caagtacctc aaacgatgat gataagagcg
     7981 atgatgcttt tgatatcgtt gtgattggtg gaggtcctgc tggttatgtt gcagccatta
     8041 aagctgccca attcggcggt aaggttgccc ttgttgagaa atctgaactt ggtggaacct
     8101 gcttaaaccg tggatgtatt ccaaccaaga cctaccttca taacgctgaa attattgaaa
     8161 atatcggtca tgctgcaaat cgtggtatcg tgattgaaaa tcctaacttc actgttgata
     8221 tggaaaaact tttagaaact aaatctaaag ttgttaatac tcttgttggt ggtgttgcag
     8281 gacttcttcg tagctacgga gttactgttc ataaaggaat tggtacaatc actaaagaca
     8341 agaacgtctt ggtaaatggc tctgaattgc ttgaaaccaa gaaaatcatt cttgctggtg
     8401 gttcaaaagt cagcaagatc aacgtccctg gtatggaatc tccacttgtc atgactagtg
     8461 atgacattct tgaaatgaac gaagtgccag aaagccttgt tatcatcggt ggtggagttg
     8521 tcggtatcga actcggtcag gccttcatga catttggttc aaaagtgact gttatcgaaa
     8581 tgatggactg tatagttcca gctatggatg cggaagtttc taagaacctt cgcttgatcc
     8641 ttgagcgtaa aggaatgacc atcttgactg gtactaaact gcaagaaatc attgaggaaa
     8701 atggtcaact tcgtatcaag gttgaaggaa aagacgatat catcgcaagc aaagctcttc
     8761 tttcaattgg tcgtatgcca gaccttgaag gtattggaga ggttgagttt gaattggatc
     8821 gtggttgtat caaggtcaac gaatacatgg aaacttcagt tccaggcatt tatgcaccag
     8881 gtgacatcaa cggtactaag atgttggctc acgcagcttt ccgcatgggt gaagtttccg
     8941 ctgaaaatgc ccttaaagga aatcatgcag ttgccaaatt gaatttgact cctgcagcca
     9001 tctacactct ccctgaagta gcagcagtag gtttgacaga agaacaagcc cgtgagaaat
     9061 acgatgttgc catcggtaag tttaactttg ctgctaacgg tcgtgctatt gcatctgacg
     9121 ctgctcaagg tttcgtaaaa gttatcgctg ataagaaata cggagaaatc cttggtgtac
     9181 acattattgg tcctgcagcc gcagaattaa taaacgaagc atcaagcatc atcgaaatgg
     9241 aaatcactgt tgaggaaatg ctgaagacca tccacgggca cccaacttac tctgaagtga
     9301 tgtacgaagc gtttgcagat gttctaggaa tggccatcca ttcacctaag aaaaaataaa
     9361 acgaagatag attagactgg aaggttattt ccagtttcta tcatataaaa taaagggata
     9421 tcatgaaata cattatcaat cattcaaatg acactgcttt taatattgcc ttggaagaat
     9481 acgcctttaa acaccttttg gatgaggatc aaatcttcct actttggatt aacaagccat
     9541 ctatcattgt tggtcgtcac cagaacacta tcgaagaaat caaccgtgat tatgttcgcg
     9601 aaaatggtat tgaggtagtt cgccgtatta gtggtggtgg agctgtttac cacgatttaa
     9661 ataacctcaa ctacacgatc atctccaaag aagatgaaaa taaggcattt gacttcaaga
     9721 gcttctcaac tccggttatc aataccctgg ctcaacttgg ggttaaagct gagttcacag
     9781 gtcgtaatga ccttgagatt gatggtaaga agttctgtgg caatgcccaa gcctatatca
     9841 acggccgtat catgcaccac ggttgcttgc tctttgacgt tgatttgtca gtcctcgcaa
     9901 atgcccttaa ggtttcaaaa gataaatttg aatcaaaagg tgtgaaatcc gtccgtgccc
     9961 gtgtaaccaa tattatcaat gaattaccaa aaaaaatcac agtcgaaaaa ttccgtgatt
    10021 tactcttgga atacatgaaa aaagagtacc cagagattac tgaatacgtc ttttcagaag
    10081 aagaattggc cgaaatcaat cacatcaagg atactaagtt tggaacttgg gactggaact
    10141 atggtaaatc acctgaattt aacgtccgtc gtggaaccaa attcactagt ggtaaggttg
    10201 aagtctttgc taacgttact gaatcaaaaa tccaagacat caagatttat ggtgacttct
    10261 ttggtattga agacgttgct gcagtagaag atgtccttcg tggggtgaaa tacgaacgtg
    10321 aagatgttct taaggcgcta aaaaccattg atatcacacg ctacttcgct ggtattagcc
    10381 gtgaagaaat cgctgaagcg gtagttggat aaatcaaaaa gaagttggcc aacttctttt
    10441 tttgatattt atttacataa tttaaaatac gtaaaattat aaactatcca gagcattctt
    10501 ttgttcatca ttaacaatat gggtatagag gtcagtgact tgtgtgctgg catgtcctag
    10561 ttggtgactg actaaaactt gtgatttagt cgcatcatag agcctagttg ctagtgtatg
    10621 gcgcagttta tggggtgtta cacgtacttt aaaatcctct gagtatttag caaccatttt
    10681 ctcaacgcta gaagcatcga tacgattagg aacacctctg tagagagtta aaaaaagggc
    10741 tgtatctgtt ttttccgttt tatagcgttg attccgaatg gccagataat tctctaaata
    10801 aggtttagca aaagcagcga cattgactga gtcacgtttg ccaccttttc gagtaacatc
    10861 aataaccatc atttttagat tgagatctct tagatctaga ttaacagctt cagataagcg
    10921 aacaccagat gccaagagaa gggcaataat ggctaaatct cgttctttat ttttgttgaa
    10981 tgatgagaga gctcgatttg aaagctgttg tgggtgctct tgatcgatat aagttagaaa
    11041 accttctgtt tcatcaccta gaaagagttt ttgcttgata ttttcagctc tggcagcaag
    11101 ggtttctttc tttttcttgg ttgaaacttt tttcattaca ttacgataga aataaggttc
    11161 cccctgatcg ttttcaacct cctcggttag atacttgtaa agactagaaa gtgctgataa
    11221 ggttcgattg atagttgtct gtgaaacacc ctgttttgtt gtattagcat tcagcaaggg
    11281 acgttcacgt agataaagga taaaggattc catgtctttc ttagacatat tttccaaaac
    11341 tgataaagga atatcggata ttttatcagc gtttgaaata ccagactcca aaacccagct
    11401 gaaaaatcgg tcatattcct taaggtattc gtacagggtt gtaaaactgt agggtacagc
    11461 cagcttagat tggtagtatt ccagaacata ccagggcatg agttgtttta gtttgtcgat
    11521 tcgttccagt aaaatctcac gtttcatgta ttttctccat aagtaagtat actagtagta
    11581 tagcatagac ttgtatattt ttcaagaaaa ttatagtttt tcagaaagat gttataggag
    11641 tttttcaaag tgataaaaag acttagacct acaaacaaaa cagaataagg aaaataataa
    11701 aaatctcacc cactaaactt ctgttaatac tacataactg aagagctata taaggagcta
    11761 tgagatacaa aacacattaa tagtacatct ggacttctaa aacattgcta gaaattgatt
    11821 tgattgtcct gattgtttta tcctatttct atttcatttc actatataat tgctattgtt
    11881 ttctttttca tgattt
//
LOCUS       DAVKJU010000059        11484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVKJU010000059 DAVKJU010000000
VERSION     DAVKJU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.059"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(154..1785)
                     /locus_tag="VW191_001637"
     CDS             complement(154..1785)
                     /locus_tag="VW191_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEU9624381.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGIGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNIIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2078..7018
                     /locus_tag="VW191_001638"
     CDS             2078..7018
                     /locus_tag="VW191_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEU9624382.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVQAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLNLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSDS
                     ALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNK
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7108..8304
                     /locus_tag="VW191_001639"
     CDS             7108..8304
                     /locus_tag="VW191_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU9624383.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8440..8967
                     /locus_tag="VW191_001640"
     CDS             8440..8967
                     /locus_tag="VW191_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEU9624384.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9044..9400
                     /gene="glnR"
                     /locus_tag="VW191_001641"
     CDS             9044..9400
                     /gene="glnR"
                     /locus_tag="VW191_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEU9624385.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9437..10783
                     /gene="glnA"
                     /locus_tag="VW191_001642"
     CDS             9437..10783
                     /gene="glnA"
                     /locus_tag="VW191_001642"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEU9624386.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10879..10998)
                     /locus_tag="VW191_001643"
     CDS             complement(10879..10998)
                     /locus_tag="VW191_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEU9624387.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
BASE COUNT         3673 a         2112 c         2489 g         3210 t
ORIGIN      
        1 atcgatttct aacaatgttt tagaaataga ggtgtactat tctagtttca atatattata
       61 tatctgcaga atactctttc agtttcattc agcctagtct tgcttaggaa aaagtaagaa
      121 gccatccaat tatgatggat agcttcttgg ttattagatt tgttctgcaa aaaccttttc
      181 agcaaggttc atagcgtggt ctgatacacg agtgtagtgt gagatgatgt cgataaagtt
      241 gactccagct tgtgttgaac attcgccttt gttgaggcgt ttgatgtggg tttttctgag
      301 aacacgttct atcttattga ttgcttcatg acgttctaca agactgcgtg ctttttcaat
      361 atcattgttt tccacactat ccagagcatc tttgataaag tcactagttt ggcggtaaac
      421 ttcctctaat tctttcaagg cggcatcaga aaattcaaca tttttccgtt gaagatagtc
      481 agtcagattg agtagagcct ccgtgtggtc tccaatccgt tccaaatcac gggaggaatc
      541 aaggatattg gtaagcactt cactttcttt ttggctgaga gcttcgcttg aaagggcaat
      601 gagataacgt gttaattgct catcgatggt gttaattgct tcttcggttt tatgcccttt
      661 ttcagcaact ttttcatcca agtcaatgat gtacttatag gaaaggtcaa aggctttagc
      721 agcgtagttt cctaagtgca agagctcttt cttagcattt cctagagcga tagatggggc
      781 ctgtttgatg aaatgttcat caagatataa gggttcgtat ttgacaacct cgtcctctcc
      841 aggaataatc ttggttacaa agtaagccag agctccgata aatggaaatt ggacaatggt
      901 gttggtaata ttaaaggttc cgtgagcaaa ggcgatggtc atttccggtg ctagatttag
      961 cgtagcttca aaccaatgaa tcaggacagt aaaaggaact agaaaaataa cgcagacgac
     1021 tgttccgata atgttgaagg caacatgagc tcctgctacc cgtttagctg caatattagc
     1081 ccctaaagag gcaatgatgg ctgtaatggt tgtcccgata ttgtcaccaa atagaactgg
     1141 caaagctccc tgtaggtcaa ttagattgcc ggcgtagagg ttttgtaaaa tcccaatggt
     1201 agccgaagaa gcttgaatta gcaaggtcaa gccagtaccg acaaagacac ccaaaacagg
     1261 attcttactt agctcaatca tatagtcctt aaagacctgt aaatccttga gtggagccat
     1321 tgcgccgctc atgagattga gggcaaaaaa gataccaccg ataccaaaga ggatgcgtcc
     1381 gatattattg actgtccgat tttttgtaaa aaagagacag acggcaccga taaagagcat
     1441 aggtagggca tagttaccta atttaaaacc gatgagaaag gatgtgacag ttgtcccaat
     1501 attagcaccc atgacaatcc cgatagcctg acgtaaggtt aagagaccgg cactgaccag
     1561 gccgactgta ataactgtta caccagaact agactgaatt agagcagtca tcccaatacc
     1621 aaccagaact ccaaagaaag gattactagt atatttgtca atgtaaaaac gaaggcgatc
     1681 tccagcagct tgttgcaaac cgtctcccat ggtcttgata ctatataaga atagccccag
     1741 accacctaaa aagtgaaata aaatttcctg ccaattaatg gacatttctt tttcctccga
     1801 aaaataatcg cggaatatct cctattctat tttaaaggat aaaagtaaat ctaacaagtg
     1861 ttaacgtcaa gtttgtaaaa gaaaattata attgataagt gtattatttt taagagtttt
     1921 taaccttatt ttagatttat gaaagcagct attgtttttt gtctaaaaat gttttaggat
     1981 aaaaatgtga ttatcatggt tctagagttt gataaaaaac tacagaatat aaagcgcttt
     2041 cttgaaaaca acaaaatcaa tcttttagga ggagaaaatg aagaatccat tttttgaaag
     2101 acgttgtcgt tacagtattc gtaagttatc agtaggagcc tgctcgctga tgattggtgc
     2161 tgttttattt gctggtccag ccttggctga agaaactgca gttcctgaaa atagcggagc
     2221 taatacagag cttgtttcag gagagagtga gcattcgacc aatgaagctg ataagcagaa
     2281 tgaaggggaa catgctagag aaaacaagct agaaaaggca gaaggagtag cgacagcatc
     2341 tgaaactgca gaagcagcta gcgcagctaa accagaggaa aaagcaagtg aggtggttgc
     2401 agaaacacca tctgcagaag caaaacctaa gtctgacaag gaaacagaag caaagcccga
     2461 agcaactaac caaggggatg agtctaagcc agcagccgaa gctaataaga ctgaaaaaga
     2521 agtccagcca gatgtcccta aaaatacaga aaaaacatta aaaccaaagg aaatcaaatt
     2581 taattcttgg gaagaattgt taaaatggga accaggtgct cgtgaagacg atgctattaa
     2641 ccgcggatct gttgtcctcg cttcacgtcg gacaggtcat ttagtcaatg aaaaagctag
     2701 caaggaagca aaagttcaag ccttatcaaa caccaattct aaagcaaaag accatgcttc
     2761 tgttggtgga gaagagttca aggcctatgc ttttgactat tggcaatatc tagattcaat
     2821 ggtcttctgg gaaggtctcg taccaactcc tgacgttatt gatgcaggtc accgtaacgg
     2881 ggttcctgta tacggtacac tcttcttcaa ctggtctaat agtattgcag atcaagaaag
     2941 atttgctgaa gctttgaagc aagacgcaga tggtagcttc ccaattgccc gtaaattggt
     3001 agacatggca aagtattatg gctatgatgg ctatttcatc aaccaagaaa caactggaga
     3061 tttggttaaa cctcttggag aaaagatgcg ccagtttatg ctctatagca aggaatatgc
     3121 tgctaaggta aaccatccaa tcaagtattc ttggtatgat gccatgacct ataactatgg
     3181 acgttaccat caagatggtt tgggagaata caactaccaa ttcatgcaac cagaaggaga
     3241 taaggttccg gcagataact tctttgctaa ctttaactgg gataaggcta aaaatgatta
     3301 cactattgca actgccaact ggattggtcg taatccttat gatgtatttg caggtttgga
     3361 attgcaacag ggtggttcct acaagacaaa ggttaagtgg aatgacattt tagacgaaaa
     3421 tgggaaattg cgcctttctc ttggtttatt tgccccagat accattacaa gtttaggaaa
     3481 aactggtgaa gattatcata aaaatgaaga tatcttcttt acaggttatc aaggagaccc
     3541 cactggccaa aaaccaggtg acaaagattg gtatggtatt gctaacctag ttgcggaccg
     3601 tacgccagcg gtaggtaata cttttactac ttcttttaat acaggtcatg gtaaaaaatg
     3661 gttcgtagat ggtaaggttt ctaaggattc tgagtggaat tatcgttcag tatcaggtgt
     3721 tcttccaaca tggcgctggt ggcagacttc aacaggggaa aaacttcgtg cagaatatga
     3781 ttttacagat gcctataatg gcggaaattc ccttaaattc tctggtgatg tagctggtaa
     3841 gacagatcag gatgtgagac tttattctac taagttagaa gtaactgaga agaccaaact
     3901 tcgtgttgcc cacaagggag gaaaaggttc taaagtttat atggcattct ctacaactcc
     3961 agactacaaa ttcgatgatg cagatgcatg gaaagagcta accctttctg acaactggac
     4021 aaatgaagaa tttgatctca gctcactagc aggtaaaacc atctatgcag tcaaactatt
     4081 tttcgagcat gaaggtgctg taaaagatta tcagttcaac ctcggacaat taactatctc
     4141 ggacaatcac caagagccac aatcgccgac aagcttttct gtagtgaaac aatctcttaa
     4201 aaatgcccaa gaagcggaag cagttgtgca atttaaaggc aacaaggatg cagatttcta
     4261 tgaagtttat gaaaaagatg gagacagctg gaaattacta actggctcat cttctacaac
     4321 tatttatcta ccaaaagtta gccgctcagc aagtgctcag ggtacaactc aagaactgaa
     4381 ggttgtagca gtcggtaaaa atggagttcg ttcagaagct gcaaccacaa cctttgattg
     4441 gggtatgact gtaaaagata ccagcctacc aaaaccacta gctgaaaata tcgttccagg
     4501 tgcaacagtt attgatagta ctttccctaa gactgaaggt ggagaaggta ttgaaggtat
     4561 gttgaacggt accattacta gcttgtcaga taaatggtct tcagctcagt tgagtggtag
     4621 tgtggatatt cgtttgacca agccacgtac cgttgttaga tgggtcatgg atcatgcagg
     4681 ggctggtggt gagtctgtta acgatggctt gatgaacacc aaagactttg acctttatta
     4741 taaagatgca gatggtgagt ggaagctagc taaggaagtc cgtggcaaca aagcacacgt
     4801 gacagatatc actcttgata aaccaatcac tgctcaagac tggcgcttga atgttgtcac
     4861 ttctgacaat ggaactccat ggaaggctat tcgtatctat aactggaaaa tgtatgaaaa
     4921 gcttgatact gagagtgtca atattccgat ggccaaggct gcagcccgtt ctctaggcaa
     4981 taacaaggta caagttggct ttgcagatgt acaggctgga gcaactatta ccgtttatga
     5041 taatccaaat tctcaaactc cgctcgcaac cttgaagagc gaagttggag gagacctagc
     5101 aagtgcacca ttggatttga caaatcaatc tggtcttctt tattatcgta cccagttgcc
     5161 aggcaaggaa attagtaatg tcctagcagt ttccgttcca aaagatgaca gaagaatcaa
     5221 gtcagtcagc ctagaaacag gacctaagaa aacaagctac gccgaagggg aggatttgga
     5281 ccttagaggt ggtgttcttc gagttcagta tgaaggagga actgaggacg aactcattcg
     5341 cctaactcac gcaggtgtat cagtatcagg ttttgatacg catcataagg gagaacagaa
     5401 tcttactctc caatatttgg gacaaccggt aaatgctaat ttgtcagtga ctgtcactgg
     5461 ccaagacgaa gcaagtccga aaactatttt gggaattgaa gtaagtcagg aaccgaaaaa
     5521 agattaccta gttggtgata gcttaaactt gtctgaagga cgctttgcag tggcttatag
     5581 caatgacacc atggaagaac attcctttac tgatgaggga gttgaaattt ctggttacga
     5641 tgctcaaaag actggtcgtc aaaccttgac gcttcattac caaggccatg aagttagctt
     5701 tgatgttttg gtatctccaa aagcagcatt gaacgatgag tacctcaaac aaaaattagc
     5761 agaagttgaa gctgctaaga acaaggtggt ctataacttt gcttcaccag aagtaaaaga
     5821 agctttcttg aaagcaattg aagcggccga acaagtgttg aaagaccatg aaactagcac
     5881 ccaagatcaa gtcaatgacc gacttaataa attgacagaa gctcataaag ctctgaatgg
     5941 tcaagagaaa tttaaggaag aaaagacaga acttgatcgc ttaacaggtg aggttcaaga
     6001 actcttggct gccaaaccaa accatccttc agattctgcc ctagctccgc ttcttgagaa
     6061 aaacaaggcc ttggttgaaa aagtagattt gagtccagaa gagcttgcaa cagcgaaaca
     6121 gagtctaaaa gatctggttg ctttattgaa agaagacaag ccagcagtct tttctgatag
     6181 taaaacaggt gttgaagtac acttctcaaa taaagagaag actgtcatca agggtttgaa
     6241 agtagagcgt gttcaagcaa gtgctgaaga gaagaaatac tttgctggag aagatgctca
     6301 tgtctttgaa atagaaggtt tggatgaaaa aggtcaagat gttgatctct cttacgcttc
     6361 tattgtgaaa atcccaattg aaaaagataa gaaagttaag aaagtatttt tcttacctga
     6421 aggcaaagag gcagtagaat tgacttttga acaaacggat agtcatgtta tctttacagc
     6481 accacacttt actcattatg cctttgttta tgaatctgct gaaaaaccac aacctgctaa
     6541 accagcacca caaaacaaag tccttccaaa acctacttat caaccggctt ctgatcaaca
     6601 aaaggctcct aaattggaag ttcaagagga aaaggttgcc tttcatcgtc aagagcatga
     6661 aaatgctgag atgctagttg gggaacaacg agtcatcata cagggacgag atggactgtt
     6721 aagacatgtc tttgaagttg atgaaaacgg tcagcgtcgt cttcgttcaa cagaagtcat
     6781 ccaagaagcg attccagaaa ttgttgaaat tggaacaaaa gtaaaaacag taccagcagt
     6841 agtagctaca caggaaaaac cagctcaaaa tacagcagtt aaatcagaag aagcaagcaa
     6901 acaattgcca aatacaggaa cagctgatgc taatgaagcc ctaatagcag gcttagccag
     6961 cctcggtctt gctagtttag ccttgacctt gagacggaaa agagaagata aagattaaat
     7021 atcgaaaaat cttgtgaaat ctttccttat atttccaaag tgtgatataa tagtttcgaa
     7081 taaaataaat aaaggagttt ttgtaacatg gcaaaactta ctgttaaaga cgttgacttg
     7141 aaaggtaaaa aagtcctcgt tcgtgttgac ttcaacgtac cattgaaaga tggcgtaatc
     7201 actaacgata accgtatcac agcagctctt ccaactatta agtacatcat cgaacaaggt
     7261 ggacgtgcaa ttcttttctc tcaccttgga cgtgtgaaag aagaagctga taaagctggt
     7321 aaatcacttg ctcctgtagc agctgacttg gcagcaaaac ttggtcaaga tgttgttttc
     7381 ccaggtgtca ctcgtggtgc tgaattggaa gcggcaatca acgctcttga agatggacaa
     7441 gttctcttgg ttgaaaacac tcgttacgaa gatgttgacg gcaagaaaga atctaaaaac
     7501 gatcctgaac ttggtaaata ctgggcatca cttggagatg gtatcttcgt aaacgatgca
     7561 ttcggtacag ctcaccgtgc acacgcatct aacgttggta tctcagcaaa cgttgaaaaa
     7621 gcagttgctg gtttccttct tgaaaacgaa attgcctaca tccaagaagc agttgaaact
     7681 ccagaacgtc cattcgtggc tatccttggt ggttcaaaag tttcagacaa gatcggtgtt
     7741 atcgaaaact tgcttgaaaa agctgataaa gtccttatcg gtggtgggat gacttacaca
     7801 ttctacaaag cacaaggtat cgaaatcggt aactcacttg tagaagaaga caaattggat
     7861 gttgcgaaag ctcttcttga aaaagcaaat ggtaaattga tcttgccagt tgactcaaaa
     7921 gaagctaacg catttgctgg ttacactgaa gtgcgtgaca ctgaaggtga agcagtttct
     7981 gaaggcttcc ttggtcttga catcggtcca aaatctatcg ccaaatttga cgaagctttg
     8041 actggtgcca aaacagttgt atggaacgga cctatgggtg tatttgaaaa cccagatttc
     8101 caagctggta caatcggtgt gatggacgct atcgtgaaac aaccaggagt taaatcaatc
     8161 atcggtggtg gtgactcagc tgccgcagcg attaaccttg gccgtgcaga caagttctca
     8221 tggattagta cgggtggtgg agcatcaatg gaacttcttg aaggtaaggt tcttccagga
     8281 cttgcagcct tgacagaaaa ataagatttt ataaacaaat caaagaagag agggatgaga
     8341 gttcctcttt tcttttgctt aaaataaaaa cgcttcctct caactattac tcataaaaat
     8401 caccgattta tgataaaatg gaaatagaaa gttgagatta tgagttattt taaaaaatat
     8461 aaattcgata aatcccagtt caaacttggt atgcgaacct ttaaaacagg tattgctgtt
     8521 tttctagttc tcttgatttt tggctttttt ggctggaaag gtcttcaaat tggtgctttg
     8581 acagccgttt ttagcctgag ggagagtttt gatgagagtg ttcattttgg gacttcgcgt
     8641 attctaggaa atagtatcgg tggactctat gccttggtct tcttcttatt aaataccttt
     8701 ttccacgaag ccttttgggt gaccttggta gttgttccaa tctgcaccat gttaaccatt
     8761 atgacaaatg tagccatgaa taacaaagca ggggttattg gtggtgtagc agctatgtta
     8821 atcattaccc tatcaattcc aagtggtgag acaatttcgt acgtgtttgt gcgtgtatca
     8881 gaaacgttta tgggagtttt tgtcgcaatt atcgtaaatt acgatattga tcgtattcgt
     8941 ctctttttag agaaaaaaga aaaataatgt tacattttat aacattatca attgacgttt
     9001 gtcttttttt agactataat agacagaaag aaggaaattg taaatgaagg aaaaagaatt
     9061 tcgccgaaat atggctgttt ttcctattgg tagtgttatg aagttgaccg atctatcggc
     9121 gcgtcagatt cgttattatg aagatcaaga gttgatcaag cccgatcgaa acgaaggaaa
     9181 tcgtcgcatg tattccttga atgacatgga tcgtctgctt gaaatcaaag attatatctc
     9241 tgaaggttat aatatcgctg ccattaagaa aaaatatgct gaacgtgaag cgaaatccaa
     9301 gaaagcggtt agtcagactg aagtacgtcg tgcacttcac aatgaactcc tccaacaggg
     9361 gcgttttgct tcagtacagt caccttttgg tcgcggttag gcaaccgcaa gtagtcatag
     9421 attaaggaga aaacttatgc caatcacagc tgcagatatt cgtcgtgaag tcaaggaaaa
     9481 aaatgttacc tttattcgtc ttatgttctc agatattttg ggaaccatga aaaacgtcga
     9541 aattcctgct acagatgaac agttagataa ggtcttgtcg aacaaggtta tgtttgatgg
     9601 atcttctatt gaaggttttg tacgtatcaa tgagtcggat atgtacttgt acccggactt
     9661 ggatacatgg acagtcttcc cttggggaga tgaaaatgga agtgttgcag gtctgatctg
     9721 tgatgtctat acaacagaag gtgaaccatt tgcgggtgac cctcgtggta atttgaaacg
     9781 agctcttcgt cacatggaag aagttggatt caaatccttc aaccttggtc cagagccaga
     9841 attcttccta tttaagttgg atgaaaatgg ggacccaaca cttgaagtga atgacaaggg
     9901 tggctacttt gatttggcac ctactgacct tgcggacaac acacgtcgtg agattgtgaa
     9961 tatcttgacc aaaatgggat ttgaagtaga agcgagtcac cacgaggttg cggttggaca
    10021 gcatgagatt gactttaagt acgatgaagt tctccgtgct tgtgataaga ttcaaatctt
    10081 taagcttgtt gttaaaacca ttgctcgcaa acacggactt tacgcaacat ttatggcgaa
    10141 gccaaaattt ggtattgctg gatcaggtat gcactgtaat atgtccttgt ttgatgcaga
    10201 aggaaataac gccttctttg atccaaatga tccaaaagga atgcagttgt cagaaacagc
    10261 ttaccatttc ctaggcggtt tgatcaagca tgcttacaac tatactgcca tcatgaaccc
    10321 aacagttaac tcatacaaac gtttggttcc aggttatgaa gcgcctgttt acattgcttg
    10381 ggctggtcgt aaccgttcgc cacttgtgcg cgtacctgct tcacgtggta tgggaactcg
    10441 tcttgagttg cgttcagtgg atccaatggc gaacccttac gttgctatgg ctgttctttt
    10501 ggaagttggt ttgtatggta ttgaaaataa aatcgaagca ccagctccta tcgaagaaaa
    10561 tatctacatc atgacagcag aagagcgcaa ggaagctggt attacagacc ttccatcaac
    10621 tcttcacaac gctttgaaag ctttgacaga agatgaagtg gttaaagctg ctctcggaga
    10681 tcacatctat actagcttcc ttgaagccaa acgaatcgaa tgggcaagtt atgcaacctt
    10741 cgtttcacaa tgggaaattg ataattattt agacctttac taatactaat atagaagaaa
    10801 gattgcctgt gggtaaacag tcacacagca gtctaagcca tcaaatacct ataaccctta
    10861 aaatagcgat ttcggtcgct atttattttt cgaaaaatcc tcttgactct tttgcgaatt
    10921 agatttatca tcagacgtgt agaagaaaat taaggagtca ctatggaaaa attcaatttc
    10981 aaaaacaata tcgggcaaga aaacaagtta cttcaaatcg aaatctacaa gtttactaac
    11041 ttttgcaagc ttcaaaatta cacatctgta aatatttttt cgaaagatat atttgaagcc
    11101 atagtaaatt gagactagaa tagtacacta catctgctaa aaatttctag aaattaattt
    11161 aactttccta atcgatttgt ttacatctta tttcaatcta ctatattatc tcaataaatg
    11221 atgatggcga ctgttttgtt tgtgcttgtc gatttttgaa tcctgatcga gcggaagaat
    11281 atttctgccg aggttgtcaa ggggaaaatc ctgaagatat tgaattttat gatgaacaat
    11341 tacaggcaga aaaggtggag gctttgaata ttcgattgga agtaaaaaaa ttgaattatc
    11401 tacaatctca tcaacaataa acctatttca accccattat ctataggcgt atttttacgc
    11461 ctttttatat acttgaagca cata
//
LOCUS       DAVKJU010000060        11161 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVKJU010000060 DAVKJU010000000
VERSION     DAVKJU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.060"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(46..1935)
                     /locus_tag="VW191_001644"
     CDS             complement(46..1935)
                     /locus_tag="VW191_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEU9624388.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFRERTVEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVKEKEQSKQLLTWIFPSILSISLLLSICFYFIYRRKTAPSIK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKALQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2073..3633
                     /locus_tag="VW191_001645"
                     /pseudo
     CDS             2073..3633
                     /locus_tag="VW191_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
     gene            3633..4373
                     /locus_tag="VW191_001646"
     CDS             3633..4373
                     /locus_tag="VW191_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9624389.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4482..4697)
                     /locus_tag="VW191_001647"
     CDS             complement(4482..4697)
                     /locus_tag="VW191_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624390.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(5567..6817)
                     /gene="ilvA"
                     /locus_tag="VW191_001648"
     CDS             complement(5567..6817)
                     /gene="ilvA"
                     /locus_tag="VW191_001648"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEU9624391.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6867..7223)
                     /locus_tag="VW191_001649"
     CDS             complement(6867..7223)
                     /locus_tag="VW191_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9624392.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7461..7697)
                     /locus_tag="VW191_001650"
     CDS             complement(7461..7697)
                     /locus_tag="VW191_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624393.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7804..8826)
                     /gene="ilvC"
                     /locus_tag="VW191_001651"
     CDS             complement(7804..8826)
                     /gene="ilvC"
                     /locus_tag="VW191_001651"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEU9624394.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPSLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8892..9368)
                     /gene="ilvN"
                     /locus_tag="VW191_001652"
     CDS             complement(8892..9368)
                     /gene="ilvN"
                     /locus_tag="VW191_001652"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEU9624395.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9361..11061)
                     /locus_tag="VW191_001653"
     CDS             complement(9361..11061)
                     /locus_tag="VW191_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEU9624396.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3371 a         2546 c         1925 g         3319 t
ORIGIN      
        1 ctatcttcca ttatatcata ctttttcagt ctatggtagc tctctttaaa aggctccgat
       61 actgccgcca cctccgcctc cagagaagcc accgccagaa cttccacttc cagaagatac
      121 ggagtaggta cttgctgtat ttgcgacact agcataatgg ctcatttgcg cgcttgaatg
      181 ataaaacata ctgtgccagc cataagctac atagagattg atatctggat tttcaacttg
      241 aatctgatga accttcatca aatgactaac cttgtccgca tagccaaata aggttgcata
      301 gaccaagaga cgattccaaa caacaatact ttccaattca gcctgatcca atcgtgcaat
      361 ctcacgcaac atattttcaa aactggtcca gagatagtag acctcagctc ccgcttcatt
      421 tagaacacca tcacgattat ctagtcgaag cttccaataa tagaaaacag acaaaactaa
      481 ccctagaaaa ccaagtattg gcaaagggag gtaaagatag ccatgaacgt ctaaactgta
      541 caagaacaaa ccaaatccga taaatagggg caagatagtc aagacaccca tacccacttg
      601 caaagccttt tccaaaccag ttaaaggacg atagtaatct gggagccccc agaaggaaac
      661 tcgttttctc actccttctt gcatctggtt caatacctct tcaaaagaag atttgagttg
      721 aagccctctt gcttgaatcc gtttttcatc agaaactttg gctctacgat aaagactatc
      781 agataccttg taatccgcaa acaaattgga aagagtttct tcttttttac ctgaaaaagc
      841 tagatttagg cagtctttct caaagcttga caaaccatct tcttttacta gcctcaaacc
      901 aactgcatct ccttctgaaa tgatagagac attcccacgg tctatcacat ctagcaaggt
      961 agcttgaata agttgatcaa aggtgaattt tccagctccc ttgaccaagg gactcacttc
     1021 ctccaaggag gtcgagtaga ctgcttctga taaaaccata ggctctaatt ccattggggg
     1081 ttcatagaga cgatgatttt tagcatattt gattgaagga gcggtctttc ttctatagat
     1141 aaaatagaaa cagatgctca ataacaagga gatggaaagg atcgaaggga agatccaagt
     1201 aagtagttgt ttactttgct ctttttcttt aacgatcgaa tcctctatct tattaaactc
     1261 ttctaaacga ttccctttca agccctgatc cctagcgcta gcaaaatcag ttcgcggcca
     1321 gtaggcatgc aattcaactc cacgcttagc cggaagattg tctaaacgaa tagtataatc
     1381 aaggttactc ttttcaaccg ttctctctct aaaaagtttc cctgtatgga aaaagagttt
     1441 ctcagccccc ttgtctcccc ttacatgaaa ttcaaacttt tcaatagact ctgaactatc
     1501 tgtcagaggt tgccaattta attcagcgat atcatcataa aggaaaagta aattttttaa
     1561 gttccagacg aggtcaactt caactatgtc gccctcctga cctggattat agactctcac
     1621 agtataacca tccgcttctt ctgttacttc gctagtcaca tctgctagtt ctgcaccgtt
     1681 tttcgcggcc tgaatctttg gatgagggtc aatgtcaaac ccgctaggca tcttaccagc
     1741 acgtccaagt cccacgattt ggcccttaaa gtcctcctca aactggtaaa ctatcttctg
     1801 tctaaactct gccgtattgt ctgcatgaat atacaaatcc ccttgataag agtttatctt
     1861 gaaatcaatg gcaaaaacag agagtggaag aaggcaaaac aagcctaaca ccagtaagaa
     1921 aaaagttttt ttcatcaact aagccccctt tttatctttc ttatcattat agcatttttt
     1981 cgtaagtaaa gacttactta tgttgaaaaa ttacgcattt ttcgataaaa taggagacag
     2041 ttatttttta atagatcaga ataggagaaa tcatgagaaa aatatactta tctattttca
     2101 caagtctctt gctgatgcta ggacttgtca atgttgctca agccgatgaa tatttacgca
     2161 tcgggatgga agcagcatat gctcccttta actggaccca ggatgatgat agcaacggag
     2221 ctgtcaaaat cgatgggacc aatcagtatg ccaacggata cgatgttcaa atcgccaaga
     2281 aaatcgctaa ggacttaggt aaagaacctt tggttgttaa aaccaagtgg gaaggtctag
     2341 tccctgccct tacttctggt aagattgaca tgattatcgc aggtatgagt ccaactgcag
     2401 aacgcaaaca agaaattgcc ttttcgagca gttactacac tagcgaacca gttctactgg
     2461 tcaaaaaaga ttctgcctat gcaagtgcta aatctttgga tgactttaat ggtgcgaaaa
     2521 tcacttctca acaaggggtc tacctttatg acttgattgc acaaatcaca ggtgctaaaa
     2581 aagaaacagc catgggagac ttcgctcaaa tgcgccaagc tcttgaggct ggtgtcatcg
     2641 acgcctatgt ttctgaacgc ccagaagctc tgactgccga agccgctaac tctaagttca
     2701 agatggtcca agtagaacca ggtttcaaaa ctggggaaga agatacagct atcgccattg
     2761 gacttcgtaa agatgagtat tagccaaatc aatgccagca ttgaaaccat ttcaaaggac
     2821 gaccaagttg ccctgatgga tcgtatgatc aaggaacaac ctgccgaagc tacaacaact
     2881 gaagagacta gcagtagttt ctttagccaa gttgctaaaa ttctttctga aaactggcaa
     2941 caactcttgc gtggtgctgg tatcactctt ttaatctcta tcgtcggaac catcacaggt
     3001 ctcattattg gacttgccat tggtgttttc cgtactgctc ctctctctga aaacaaagtc
     3061 atttacagcc tacaaaaact agtcggctgg atcctcaacg tctacattga aattttccgt
     3121 ggtacgccaa tgattgttca atcgatggtt atctactatg gaactgccca agctttcggt
     3181 atcaaccttg accgtacact ggctgctatc ttcatcgttt caatcaatac cggtgcctac
     3241 atgactgaaa tcgtccgtgg tggtatccta gcagttgaca agggacaatt tgaagctgcg
     3301 actgctcttg gtatgaccca taaccagacc atgcgtaaga ttgtcctacc tcaggtagtc
     3361 cgtaacatcc tacctgcaac tggtaatgaa tttgtcatca atatcaaaga tacatctgta
     3421 ttgaacgtta tctctgttgt cgaactttat ttctcaggaa ataccgtggc aacacaaacc
     3481 tatcaatact tccagacatt tacaatcatc gccgtgattt actttgtcct caccttcacc
     3541 gtaacacgta tcctacgatt tatcgagcgc cgaatggaca tggataccta tactacaggt
     3601 gctaaccaaa tgcaaacgga ggatttgaaa taatgacaca agcaatcctt gaaattaaac
     3661 acctcaaaaa atcctatgga caaaacgaag tgctaaaaga catttcactc actgtccaca
     3721 agggagaggt catctctatc atcggaagct ctggaagcgg aaaatcgacc ttcctacgct
     3781 ccattaacct acttgaaaca ccaactgatg gacaaatcct ttatcatgga caaaacgtcc
     3841 tcgaaaaagg ctatgacctc acgcaatacc gtgaaaagtt agggatggtt ttccaatcct
     3901 ttaacctctt tgaaaatctc aacgttcttg aaaacacaat cgtcgctcag acaactgtcc
     3961 ttaaacgcga acgcacagaa gctgaagaaa ttgccaaaga aaacctggaa aaggtcggca
     4021 tgggagaacg ctactggcaa gctaaaccca aacaactctc aggtggtcaa aaacaacgtg
     4081 tggccattgc tcgtgccctt tcaatgaatc ctgacgccat tctctttgat gaaccaacat
     4141 cagctctcga tccagaaatg gttggagagg tcctcaaaat catgcaagat ctggctcagg
     4201 aaggcttgac catgattgtc gtaacccatg aaatggaatt tgcccgtgat gtctctcacc
     4261 gtgttatctt tatggataag ggcgtgatcg ctgaagaagg taaaccagaa gacctcttca
     4321 ccaatcctaa agaagaccga acaaaagagt tccttcaacg ctatctcaaa taaaaagaaa
     4381 aggctgcatc aaccgtgcag tctttttgct gtcctcaaaa tgaaaaggca gattctctat
     4441 ctctactact tgaaggtcaa gaatccaccg ttatccaaag attaatcttc aaatttttca
     4501 tgattttttt catagaaatc aattaagcct agggctgttt caaatgaaat attttttact
     4561 tttgcacgac cctgcgccag agcaatgata gacatttcac gcgcattcgt ttctttagag
     4621 atacggtagc ctgtgatttt cttatcacga acccagccaa caactgattc tactttttca
     4681 aagtttgact tagccatgtt tttctcctat tttctccttt ataatattta attgtatacg
     4741 tttttactac ttttgtcaat aaaaaaacat atattcaacg aaaataatta gttcagaatt
     4801 ttcttacttc ctttttaaat ccgataatat ataggttttg ctagcctcta caccataaat
     4861 atttgctaat aaaaatactg tattattacc ctcttaaggt gcgaaattat tcaagttctt
     4921 aaaaaatgct tctctatttc cgtaagatag cttatttctt cgatttaaga actgaagatg
     4981 atagttctaa cctttagaaa tccaagcctt cactagctca aaaactccta tctttcttcc
     5041 tcaataaatc atctgagaga taggtattcc ttatgtcctt caaattcatc actcctttgg
     5101 atattgaggt gctagcctag aagcagacac ccgattatct aaaccatcct taatagagat
     5161 gacaagacag ataagctcag ttaattactt atatatcttt ttaatatggt atatttgtca
     5221 acaaaaagaa atcaaatcat accttatact caatgaaaat caaagagcaa actaggaagc
     5281 tagccgcaag ttgctcaaaa cactgttttg aggttgcaga tagagctaac gtggtttgaa
     5341 gagattttcg aagagtatta aagacagtgt gactatgctt gtcaatagat aggaaatcaa
     5401 aatgaaggat ggaaatctga gccaaacatc ataagtgcta taatgaacag aagtaagaat
     5461 tgcacgataa gttttctaaa accataggaa tcgtcccctt tcttcatctt cattatagca
     5521 catatacatc agttgtacaa ataatatgat atttttttat tagtcctcag acaagcatat
     5581 tataaagcgt ttcattacca tttaagttaa tataagctgg atcaaaacct tccattctac
     5641 gaatcaaacc tgcataatca tgcttatctg ctaaagcgat cccaattaat actgggcctg
     5701 ttcccttgct agctcgtttg atatactcaa aacgtgtgat atcatcattt ggccccagga
     5761 tatcatttac aaactcacgc aaagctcctg gacgttgtgg gaaattgacc acaaagtaat
     5821 gtttgatacc atcataaatc aaggcacgct cttccatttc tggcatacgg ttgatatcat
     5881 tatttcctcc agaaatgata caacaaatgg ttttcccctt aatatattca gctaaaacct
     5941 ctaaagaggc gatactagcc gctccagcag gttctgcgac tatcccttgc ttagagtaaa
     6001 ggtcaatcaa ggtttcagaa atcaatccct catcgacacc taccaaagtt ttaatatgtt
     6061 gacgagttgc ttcataggtc aactgaccta ccttttgcac agcaatccca tccgcaaatt
     6121 tatcaatttc cttgagtttt actggacctc cagcctcaaa ggcagctttc atggaacgcg
     6181 ctccattcgc ctctactccg ataacctcaa tctctggact tgtttccttg atataggtag
     6241 aaaccccggc aatgagaccg ccaccaccaa caggaaccaa gacagcatca aaatcaatcg
     6301 attcttttcg agcttcttct aaaatctcat aagcaactgt tccttgacct gcttgaacat
     6361 gagcatcatc aaaaggatca ataaaggtac gattttcaga gactgtaaat tcttgagctg
     6421 ctttggctga ggcatcaaag gtatctccaa ctagtttaat agttacaaaa tccccaccaa
     6481 aaaagcgaac ctgaccaatc ttttgttgtg gcgtagtaat gggcataaag atagtagcag
     6541 gaattttcat ttcattacaa gtataggcta ctccctgcgc atgatttccc gcagaagcgc
     6601 agactacccc acgttcacgt tcttccttgc tgagctggga aatggcataa taggcaccac
     6661 gaattttaaa ggagcgaaca cgctgggcat tttctttttt caaataaatc ttagcaccat
     6721 acttctccga taaataatga tcgtaatcca gtggagtatt cacaaccaca ccgttcaaga
     6781 ccttgtgagc cttgattata tcttttgaac ttaacatata aaacctcttt ttctataaaa
     6841 aacagtcact aatgactgtt ttttagttat tcatctccga aaacttcagt aacactttca
     6901 tggtatttat gagccatacg aatcgaaaac tctttaggaa gttcataacg atttgtggaa
     6961 atccaatcat ataaccattc taccaaactt tcttcactat cattacgatg gaagataaaa
     7021 tagtttaaaa tttctttata ttctacatca ttcaaatcca acgtgtctac aaattgacta
     7081 cgaggaacaa aattagaatc taattttctt agttgatctg tcaattctcc caattgtaaa
     7141 tccaactgaa aaaatagttt ttgcagttgc ctatcagttt catcacatcc tgtcttgata
     7201 gaaaacaaat tggtaatatt catatgattt caacccctaa ataagtacat ctaatatccc
     7261 acgaatagct gaagctaaag ttttcgcagt ccagtcattt aaacccgatt ctttcaatcc
     7321 agatgcaata atttgctcta tttgatataa accgccatct atcgtctcat ttattctgcg
     7381 aagaggacct tcaatatagg ataaagcttc tccgaatcta acagctgatt taggtcctaa
     7441 tgatttacca actatatttg ttaatgtttt ttatttctca aaatccattt cgctatataa
     7501 cgagcagatt tagtcgcacc agctctctca aacggagaat gaatttcaag accttcttct
     7561 ctagctattt gtgccaattt gaagaattgt tcaaaggtca tatgcaacct accatcttca
     7621 aaattagcat catcataaat gttaattaca gcattttcat catacgacca atctgcttta
     7681 ttttggtctg ctagcactat taccgacggt gtaattacat tatacaacta gaaccaataa
     7741 taacgatgca gtttttctca taatcttttt cataaaatca tctccagcgc tatatatttc
     7801 taattagtta tagattttga atgcatcatc gtcgttttta ccaacgaatg gcattgcttt
     7861 acgcaattct gcaccaactt tttcaatttc aaggttagct gcttgttcac ggtaagcagt
     7921 caattttgga cgtccagctt tatagtcatt tacaaagtca tttgcaaatt taccattttg
     7981 gatgtctgcc aagacagcct tcatattttc tttaacttgt tcagtgatta cacgtggacc
     8041 tgatacatag tcaccgtatt cagcagtgtt tgaaatagat tgacgcattt tcttgaatcc
     8101 accttcgtag atcaagtcaa cgatcaattt catttcgtga agaacttcaa agtaagccaa
     8161 ttctggagcg taacctgctt ctgtcaagac ttcgaaacct gcttcgataa gggcagtcaa
     8221 accaccacaa agtacagctt gttcaccaaa caaatcttct tcagtttctt ctttgtaagt
     8281 tgtttcaaga agacctacac gagccgctcc aacaccttta caccagtcca tagcaatgtt
     8341 tttagcattt cctgttgcat cttggtatac tgcataaaga gctggaacac caaatccttc
     8401 ttcgtaagta cgacgtacca agtgtcctgg tcctttagga gcacacatga agacatctac
     8461 atccgcagga actttgataa attcaaagtg gatgttgaaa ccatgggcaa atccaactgc
     8521 gtttccagct tccaagcttg gagcgatttc tgcttcgtac aattcttgtt gaatttcgtc
     8581 tggcgccaag atcatgataa catcagccaa cttagtagct tctgctactg tgtaagtatc
     8641 aaatccatct tcttttgctt tatcaaaaga tttacctgga cgtacaccga taataacgtc
     8701 acgacctgaa tcacgcaagt tttgagcatg cgcatgccct tgtgaaccat aaccgataac
     8761 ggcgattttt ttaccgtcaa gtgctgctac tttaacatct ttttcatatt ccatttgaac
     8821 tgtcatagct tttctctctt ttctattatt tattgccttt taggctggtt taataaattt
     8881 aagttggatt tttaatcgcg ggtaaatcca gttgcacccg ttcgagcaat attgcgaata
     8941 ccgtatgggc gaatgactcg caatagggct tcgctctttt ctgcatttcc cgtcatctga
     9001 atggtaatcg agctcggcgc tacgtctacc actgttgcac ggaaaggttg aataatcgct
     9061 aaaatctcag ctctcttctc agctggcgct gacatcttaa ccaaaatcac ctcgcgctcc
     9121 aaatgaggct tgtctgtaat atctcgaatg cgaatcacat caatctgacg attgagctgt
     9181 ttgatgattt gctccacttc atcatgagaa gcaacatcaa taataatagt gatacgcgat
     9241 acattcggat cttctgttgc tccaacagag atgctttcaa tattaacctg acgacgagat
     9301 aggacacctg taaagcgatt gaggactcct gaacgatttt gtagttttgc tgttaacatt
     9361 ctacgcatgg aactgcaccc ccaacatctc atgattactc ttaccagccg gtaccattgg
     9421 taacacctgt tccttacgag aaatatctac ctcaattagc ataggaacat cctcagtgat
     9481 gacttcaagg tcttgagcca aggtctcagg attgtcaaac ttatagtttt taataccata
     9541 agcctgcgcc atcaattgga aatcaggaag ggtatcaaag accgactctg atgttctgcc
     9601 ttcatagaag gattcctgcc actggcgaac cattccgagt gaatgattgt tcagcataac
     9661 caccttgatt ggcaccttgt aaatattcaa aatagccaac tcctggttgg tcatttggaa
     9721 accaccatcc ccaacaaaca agactacttc cttatctggg ttagcaattt tagcaccgat
     9781 tgctgctgga attccaaagc ccattgttcc caaaccacct gaagtcacta actgacgttc
     9841 attttggtag ggataatact gagctgtcca catttggtgt tgaccaacgt ctgttaccac
     9901 aatggcatct ccattcgtca attcaccaat tcgttcaata actgcttgcg gttgaaccac
     9961 acgctctttc ttgtcataag aacgaacacg attcttgtct ttagtgactt tctcaatcca
    10021 cttttcagta ttgttgtgaa ctgttggttc tgctagcaac atttgcaagg ccttcttagc
    10081 atctccaact acaggaatgt ctgcactgat aatcttgcca atctcagctg ggtcaatatc
    10141 aatgtgggca accttagcat tcttagcgaa agtcttagga ttccccgtca aacggtcatc
    10201 gaaacgagaa ccaatactaa tcataaagtc cgcttccgtc atggcaatat ttgctgcgaa
    10261 tgacccgtgc atgcctccca ttccaagaaa gagtgggtga ctcgttgcaa tcgttccttg
    10321 tcccaaaaga ctggttacca ctggaatttg atagcgttct gcaaattcat ttagttccgt
    10381 agcagcctca gcataactaa ttccaccacc agctaacaag actggctttt tagccttgga
    10441 caattgcttc aagattttct tgatttgcat atcattcggc tcaagagtcg gctgataact
    10501 tggtaaattc acttctggtg aataaatgaa gtctgtttct aaagcagata tgtcttttgg
    10561 taggtcaatt acaactggcc ctggacggcc tgtagttgcg atatggacag cttccgtaat
    10621 gatacgcgga atatcagctg tctcacgaac ttggtaattg tacttagtga ttggcatggt
    10681 aattcccacg atgtctgcct cctgaaaggc atccttccca atccctgctc gcgccacctg
    10741 acctgtaaag accaaaaggg gaacgctatc gctcatggca tccgcaatcc ctgtaatggc
    10801 atttgttgct cctggtccac tagtgacgac ggcaacaccc aactttccag ttgatttggc
    10861 ataaccttca gcttcatgca aacaaccttg ctcatggcgc cctagaatgt ggcgaatgcc
    10921 tttaaaatta tatatcgcat cataaaaagg caaaaccgca ccaccaggat aaccaaagat
    10981 ggtatcaact cctaaatcac gaagtgtttc caaaactagg tccgaccccg tcttaggaga
    11041 ttctaaactg attttctcca ttgttcccct ttctttcctc ttaaaaataa cttgttacta
    11101 tcataccatt ttttcaaaat ttttcaagat aaaagaagaa attttctgaa ttttctattt
    11161 t
//
LOCUS       DAVKJU010000061        11139 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVKJU010000061 DAVKJU010000000
VERSION     DAVKJU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.061"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            99..461
                     /locus_tag="VW191_001654"
     CDS             99..461
                     /locus_tag="VW191_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624397.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            996..1688
                     /locus_tag="VW191_001655"
     CDS             996..1688
                     /locus_tag="VW191_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEU9624398.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIKFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1741..4152
                     /locus_tag="VW191_001656"
     CDS             1741..4152
                     /locus_tag="VW191_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEU9624399.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLEAVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     RGQEYIEAARASLNDREDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSAMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4402..5103
                     /locus_tag="VW191_001657"
     CDS             4402..5103
                     /locus_tag="VW191_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624400.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNSDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5105..7813
                     /locus_tag="VW191_001658"
     CDS             5105..7813
                     /locus_tag="VW191_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9624401.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQAEQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSVASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7909..8400)
                     /gene="tpx"
                     /locus_tag="VW191_001659"
     CDS             complement(7909..8400)
                     /gene="tpx"
                     /locus_tag="VW191_001659"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEU9624402.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8523..9452)
                     /gene="psaA"
                     /locus_tag="VW191_001660"
     CDS             complement(8523..9452)
                     /gene="psaA"
                     /locus_tag="VW191_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEU9624403.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9484..10332)
                     /locus_tag="VW191_001661"
     CDS             complement(9484..10332)
                     /locus_tag="VW191_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEU9624404.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10329..11051)
                     /locus_tag="VW191_001662"
     CDS             complement(10329..11051)
                     /locus_tag="VW191_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEU9624405.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT         3283 a         2066 c         2527 g         3263 t
ORIGIN      
        1 tatacaaatt tttattttat aatatcagaa aatacttttt ttaaaaagca aatatgatac
       61 aattttattt gaaaaaaaat aaaaaaggag attttattat gaaatcaagt ggtatcgtcg
      121 gtcttgtggg aggaatttta cttcttattt gtccttttgt cttgttggga acagctgtaa
      181 atacagctgc tacaactctt aatggaggag ctactgcagg ggctttttca ggtgttgcct
      241 tactcttgaa tgccttgaag attgcaaatc ttgttcttgg tatcattgct attgtttact
      301 ataaaggaga taagcgtgta ggtgcagctc cgtctgtact aatgattgtt tctggtgaag
      361 ttagtctcat tccattctta ggatgggttg gggggattct tgctattatc ggaggatctc
      421 tattccttgc aacattgaag aaattcaaat cagaagaata aaaggtattt tagcatgaaa
      481 agaacaaaaa agtttatcgg tataggagta gctctattat ctctttctct tctagttgca
      541 tgtggaacat aaagttcaaa gaatacttca acaagtaatg atgagaagac agtagcaaca
      601 tccaatagtt caaaagaaac aatcactttc gatacaccgg ttgtaacaga cgatgcgatt
      661 gaatcaatac gcacttatgc agattatata gatctttata aaaatatttt tgatgattat
      721 tttactaaaa ctgaggaagg tttcaaaggc acagctatgg aaaataatga ctcgtttact
      781 aaactaaaag agtcaactca aaaattattc gatgcgcaga aaaaaaggtt aaataatgaa
      841 gatagaatag aaacaaccaa aaacaatgtg attgccaaac attgtcaaac agtcctttcc
      901 tttttggttt tgactagctt ttttgtgaaa aattgtgtaa aatagaatag ataaacgagg
      961 ggaaacctcg gaaaatttaa aggagaatcc atctaatggt aaaattggtt tttgctcgcc
     1021 acggtgagtc tgaatggaac aaagctaacc ttttcactgg ttgggctgat gttgatttgt
     1081 ctgaaaaagg tacacaacaa gcgattgacg ctggtaaatt gatcaaagaa gctggtatca
     1141 aatttgacca agcttacact tcagtattga aacgtgctat caaaacaact aacttggctc
     1201 ttgaagcttc tgaccaattg tgggttccag ttgaaaaatc atggcgcttg aacgaacgtc
     1261 actacggtgg tttgactggt aaaaataaag ctgaagctgc tgaacaattt ggtgatgagc
     1321 aagttcacat ctggcgtcgt tcatacgatg tattgcctcc aaacatggac cgtgatgatg
     1381 agcactcagc tcacacagac cgtcgttacg cttcacttga cgactcagtt atcccagatg
     1441 ctgaaaactt gaaagtgact ttggaacgtg ctcttccatt ctgggaagat aaaatcgctc
     1501 cagctcttaa agatggtaaa aacgtattcg taggagctca cggtaactca atccgtgccc
     1561 ttgtaaaaca catcaaaggt ttgtcagatg acgagatcat ggacgtggaa atccctaact
     1621 tcccaccatt ggtattcgaa ttcgacgaaa aattgaacgt cgtttctgaa tactaccttg
     1681 gaaaataaaa aattgtaagt ctagaattga tttctaggct ttttatgtta gtatggaagt
     1741 atgataagga ataaaaaaca agattatgta ctggcctaca agcaaccagc ttcaaccact
     1801 tacatgggtt gggaagaaga agctttaccg ataggcaatg gttctttagg agcaaaagta
     1861 tttggcctta taggggctga acggattcaa tttaatgaaa aaagtctctg gtctggaggt
     1921 ccacttcctg atagttcaga ttatcagggt ggaaatcttc aggatcagta tgttttttta
     1981 gctgagattc ggcaggcttt ggagaagaga gattacaatc tggctaagga actggctgag
     2041 cagcacctaa ttgggccaaa aacgagtcaa tatgggacct atctgtcttt tggggatatt
     2101 cacattgagt tcagccagca aggtacgact ttgtctcagg tgacggacta tcagagacag
     2161 ctgaatatta gtaaggcact tgcgacgact tcttatgtct ataagggaac gcgatttgaa
     2221 cgtgaagctt ttgcgagttt tccagatgat ctcttggttc aatgttttac taaggaaggg
     2281 ttggaaactc tagattttac tatagaacta tccttgacct gtgatttggc ttctaatgga
     2341 aagtatgagc aggaaaaatc tgattacaag gagtgtaagt tggatattac tgattctcat
     2401 atcttgatga agggaagagt taaggataat gatctgcggt ttgctagtta tctagcttgg
     2461 gaaacggatg gagatattag agtttggtca gatagggttc agatatcagg agccagttat
     2521 gccaatctct tcttggccgc taagacggat tttgcccaaa atcctgctag caattatcgc
     2581 aagaaactag atttagagca acaggtgata gacttggtgg acacagctaa agaaaagggc
     2641 tatacccaat tgaaatcaag gcatatcgag gactaccaag ccttattcca gcgtgttcaa
     2701 ttggatttgg aagctgatgt tgacgcatcc actacagatg atttgttaaa aaattataag
     2761 ccacaagaag ggcaggcttt ggaggagctg ttcttccagt atggacggta tttattgatt
     2821 agttcgtcca gagactgccc agatgctcta ccagctaacc tacagggagt ctggaatgcg
     2881 gtcgacaatc ctccttggaa ttcggactat cacttaaatg tcaatctgca gatgaattat
     2941 tggccagcct atgttaccaa tctcctagag gcggtctttc cggtcatcaa ctatgtagat
     3001 gatttgcgtg tctatggtcg tctagcggct gtaaagtatg caggaatcgt ctctcaaaaa
     3061 ggtgaggaaa atggttggtt ggttcatact caagcgactc cctttggttg gacggcacct
     3121 ggttgggatt actattgggg ttggtcacca gctgccaatg cgtggatgat gcaaaccgtt
     3181 tatgaagcct attcatttta tagggaccaa gactatctca gagagaaaat ttatcccatg
     3241 ttgagggaaa cggttcgttt ttggaatgcc tttttacata aggatcagca ggcgcagcgt
     3301 tgggtgtctt ctccgtctta ttccccagaa catgggccga tttcgattgg caatacttat
     3361 gaccaatctc tgatttggca gttatttcat gattttattc aggctgctca ggaattggga
     3421 ctggatgagg acttgttgac tgaggttaag gagaagtctg atttactaaa tcctttgcaa
     3481 atcactcaat ctggtcgaat cagggagtgg tatgaggagg aagagcagta ttttcaaaat
     3541 gagaaagtgg aggcccagca tcggcacgct tcccatctag tgggactcta tcctggcaat
     3601 ctctttagct acaggggaca agagtatatt gaagcggcac gtgctagcct caatgatcgt
     3661 gaagatggcg gcacaggctg gtccaaggct aataagatca atctctgggc gcgtttggga
     3721 gatggcaatc gagcccataa attattggca gagcagttaa aaacatccac cttgccaaat
     3781 ctttggtgta gccatcctcc ttttcagata gatggtaatt ttggtgctac tagtgccatg
     3841 gcagaaatgt tactccagtc tcatgcagct tatctggtac ctctagctgc cctacctgat
     3901 gcttggtcaa caggttctgt ttcaggctta atggcacgtg gacattttga agtgagcatg
     3961 agctgggaag ataaaaaact cttacagttg accattttat caaggagtgg aggagatttg
     4021 cgagtttctt atccagatat tgagaagagt gtgattaaaa tgaatcaaga aaaaataaaa
     4081 gcgaaatgca tggggaaaga ttgtatttcg gtggcaacag cagaaggtga tcttgttcaa
     4141 ttttattttt aagaagatgt tataaggcag taatttgaaa ctgcctttta ataaggattt
     4201 aagaatataa gcagttttca actagttgaa aaagcgttat aatgataata ggaagtaata
     4261 ctcaatgaaa atcaaagagc acaaactagg aagctagccg caggttgctc aaaacagtgt
     4321 tttgaggttg cagatggaag ctgacgtggt ttgaagagag agtttcgagg agtataattt
     4381 gtttgataga gggtgggtct gatggcttat attgagatga aacactgtta caagcgttat
     4441 caggttgggg acacggagat tgtggccaat cgtgatgtga attttgagat tgaaaagggg
     4501 gagctggtta ttatccttgg tgcttcaggt gcaggcaagt caacagttct taaccttctt
     4561 gggggaatgg ataccaatga tgaaggggaa atctggattg atggtgttaa tattgcggat
     4621 tatagttccc accagcgcac caattaccgt agaaatgatg tggggtttgt ttttcagttt
     4681 tataatctag tttctaatct gacagctaag gaaaatgtgg aactggcttc tgaaattgtg
     4741 acagatgcct tgaattctga tcaggtcttg acagatgtag gtctggctca tcgtctaaat
     4801 aactttccag cccagctttc tggaggggag caacagcgag tctccattgc acgcgcggta
     4861 gccaaaaatc ctaaaattct tctttgtgat gaacctactg gagccttgga ttatcagacg
     4921 ggcaagcagg ttttgaaaat tctccaagat atgtctcgtc aaaagggagc gacggtgatc
     4981 atcgtgactc ataatggagc tttggcgccc attgctgatc gcgtgattca tatgcacgat
     5041 gccagtgtca aggatgtggt gctcaaccag catcctcagg atattgacag tttggagtac
     5101 tagtatgatc aagcgaaaaa cttattggaa ggacttaatt cagtccttca caggctccaa
     5161 ggggcgtttt ttatccatct tgatcctgat gatgttggga tctctagcct tagtaggcct
     5221 caaagtaacc agtcccaaca tggaggcgac agctaatgct tatttaacaa ctgctcaaac
     5281 cttggatttg gcagtcatgt ctaactatgg cttggatcaa gcagagcaag aagaactaaa
     5341 acagacggag ggcgcagagg tcgagtttgg ctatttgaca gatgtgacta tggataatgg
     5401 gcaggatgcc attcggctgt actccaaacc agagcgaatt tcaacctttc agctaagaaa
     5461 gggacgactt cctcagtcag acaaggaaat cgctttggcc actcatttgc aaggccaata
     5521 cagcgtggga caggagatta gttttaaaga aaaagaagag ggtcattcct ctttaaaaga
     5581 ccatacttat accattactg gttttgtgga ttcggctgaa atcctctccc agcgagatat
     5641 gggctacgca ggaagtggaa gtgggactct gacagcctat ggggtgattt tacctagtca
     5701 atttgatcag aaagtctaca atatagctcg tttgaaatat caagatttag caggtttaaa
     5761 tgccttttca tcagcttatg aagaaaaatc taagcaacat caggaagagc ttgaacaaat
     5821 tttatcagat aatggcaagg tacgtctgca acttttgaaa aaagaaggac aagagtctct
     5881 agacaagggg caagagaccc ttgacaaggc tcagactaat ttgcaggaag gcaagcgtcg
     5941 tttagcagct gctcaagctc gtatacaggc tcaagaaagt caactagcct tgtttcctca
     6001 agttcagaga gagcaggcta gtgctcaact tacccaagcc aagcaggaat tgggcaagga
     6061 agaggacaaa ctaaagcaag ctgaacaaaa tctagcccaa gaaaaggaaa aattagaaaa
     6121 acatcagcaa gtcttggacg atttggcgga gccaaggtat caggtctata atcgtcagac
     6181 catgccaggt ggtcagggct atcttatgta tagcaatgct tcatccagta ttcgagcagt
     6241 gggcaatatc tttcctgtgg tactttatgc cgtagcagcc atggtgacct ttacgaccat
     6301 gactcgcttt gtagacgaag agcgaactca tgcagggatt tttaaggcct tgggttaccg
     6361 tagtaaggat attatcgcca agtttctcct ttatggacta gtagctggga ctgtcggaac
     6421 ggctctaggt agtatacttg gtcattattt gctagccagt gtaatttcaa gtgtcattac
     6481 aaaaggcatg gtggtgggag aaacccagat tcagttctat tggacctata gcttactagc
     6541 tcttgtcttg agctggttgg cgagtgtgtt accagcctat ctggtggctt ggagggaact
     6601 tcatgacgaa gcagcccagc ttctacttcc taaacctcct gtcaaaggag ctaaaatctt
     6661 attggagcgt atcggtttta tctggcgtcg tctcagtttt actcataagg taacagcccg
     6721 caacatcttt cgttataagc agagaatgtt gatgacaatc tttggtgtgg caggttctgt
     6781 agctctgctc tttgcaggtt tgggaatcca atcttctgta gcaggagttc cgtctaaaca
     6841 gtttcaacaa atccaacagt atcagatgct tgtctctgaa aatcctagtg cgaccaatca
     6901 ggacaaggta gagctagcag aagtgttgaa agggcaggag atactagcct accagaaaat
     6961 ctattctaaa acgctagaca aggatttcaa aggcaaggct ggtcttcaaa acattactct
     7021 tatgatgata gagaaggaag atttgactcc ctttatccat cttcaacatc atcagcagga
     7081 gctgacatta aaagatggca tcgttattac agctaaactc gcccagctgg caggtgtcaa
     7141 ggttgggcag actttagaaa ttgaaggtaa ggaactaaag gtcgctgcta ttactgagaa
     7201 ctacgttggt cactttattt atatgagtca ggctagctat gagcaacttt acggacagct
     7261 accccaagcc aacacttatc tggtctcatt aagggatacc agtgcaacta gtatcgaaag
     7321 tcaggcgggc ttgcttatga atcaatctgc ggtgtctagc gttgtccaaa atgcttcagc
     7381 cattcgactc ttcgactctg tcgctagctc actcaatcag accatgacca tcttggtcat
     7441 cgtatcggtt ctattagcta ttgtcatcct ttacaatctg accaatatca acgtagctga
     7501 gagaatccgt gaactctcca ctaccaaggt tcttggtttt cataataatg aagtcaccct
     7561 ctacatttac cgtgagacga ttgtgctatc ccttgtggga atcgtacttg gtctggtagc
     7621 tggtttctat ttacaccaat ttttgattca aatgatttcg cctgcgacta ttctctttta
     7681 tccgcaggta ggctgggaag tctatgtaat cccagtggca gcagtaagca tcattttgac
     7741 cttgcttggt ttcttcgtca attattatct gagaaaggtt gatatgttag aagccctgaa
     7801 atctgtagag taaggtagtt atttttagct gattgaactt ctatttacta atattcaaaa
     7861 atcctccgtt tcaaagagca gggaactctt tgtgacagag gattttttct atagggcttt
     7921 agcagctgca attgcggctt cgaagtttgg ctcagaattg atattatcca cgtattcaac
     7981 gtagcgaatc gtattgtcag tatcgaggac aaagactgcg cgtgctaata ggtgccattc
     8041 gttgatcaag agggcataat cgcgcccgaa agaatggtca aagtagtctg aaagcataat
     8101 ggcattgtca aggccttcag caccgcacca acgtttttga gcaaaaggta ggtccattga
     8161 aacagtcaat acgaccgtgt tgtccagtcc agctaattct tcattaaaac gacgtgtttg
     8221 agttgagcag atgcctgtat cgatagaagg aacgacactc aagacttttt tcttgccatc
     8281 aaaatcagcc agagattttt tagaaagatc tgttgtagta agagaaaaat caagcgcctt
     8341 gtcgccgact tgtagttgtt tacctgtaaa gctcacagga tttccgagaa aagttaccat
     8401 aggatactcc aatctttttt cttccattgt atctgaaaca gtcagaattt tccaatgatt
     8461 tgaccggaaa tgtgggcata gaaaaaacgc cagctcacat gagaatgacg tttttcagag
     8521 gcttattttg ccaatccttc agcaatcttg tcaaggttgt atttcatcat gctgtagtag
     8581 ctgtcgcctt ctttaccttg ttctgcgata gagtcagtaa agatttgtgc gtagattggg
     8641 atgtttgtgt cttgagaaac agttttcatt ggacggtcat ccacacttga ttctacaaag
     8701 agtgatggaa cttttgtttg gcgaagtttt tcaaccaagg tcttgatttg ttcaggagtt
     8761 ccttcttctt cagtattgat ttcccagatg taggcacttg gaacaccata ggctttagag
     8821 aagtatttga atgctccttc gctggttaca atgagtttct tttcagcagg gatcttatta
     8881 aatttatcct tactttcttt atcaagtttg tctaacttat cagtatattc tttgagattt
     8941 ttttcataga attctttatt gttagggtct ttggcgctca attgtttagc gatattttta
     9001 gcaaaaataa taccgttttc aaggttaagc caagcgtgtg ggtcttcttt tcctttttca
     9061 ttttgacctt caaggtagat aacatcaacg ccgtcgctga ctgcgaagta gtctttgttt
     9121 tcagttttct tggcattttc taccaatttt gtaaaccaag cattgccacc tgtttcaagg
     9181 ttgataccgt tatagaaaat caaatcagcc tcagaagttt tcttaacgtc ttcaggaagt
     9241 ggttcgtatt cgtgtgggtc ttgcccaatc ggaacgatac tatgaaggtc aattttgtca
     9301 ccagcaatat ttttagtaat atcagcgatg attgagtttg tagcaacaac ttttagtttt
     9361 tgaccagaag ttgtatcttt ttttccgcta gcacatgcta caagaatgat tgcagaaaga
     9421 aagagaacga gtaatgtacc taattttttc attagatcct ccaatttatt agggctttgc
     9481 cccttatttt aacaaatgtt tatttttcag tttcaaatat cgttgtttgg gagcgataaa
     9541 gaagctaatg agaaagaaac tagcagctgt aagcacgata ctagaacctg ccgcaacatt
     9601 aaaactatag ccaataaaga gtcccaaaac tgaagcagta gctccgaagg ttgaggaaag
     9661 gaaaatcatg cttttcaaac tattagcata cagataagca gttgcagctg gggtaatcag
     9721 catggctaca atcaggatag ttccgacact ttgcatggct gtcacagaca cgagagtcag
     9781 gagtaccatg agaaggtagt gatagaaatt gacaggcatt cccatggctt tagccaagag
     9841 ttcatcaaag gaagttatca agagttgctt gaagaaaatc cagattaaca agaggatggc
     9901 tgcccccaca cccatagtaa taaacatatc cgtatcttgg acggccagga tattaccaaa
     9961 aaggatatgg aaaaggtcag ttgaactttt agcgacacca atcaagatga taccgagggc
    10021 taagaaagaa gaaaaggtaa tgccgatggc ggtatcgctt ttgataatcg agtttccttt
    10081 gatgtaggta atgatgatgg cagctagcaa tccaaagaca atggctccga taaagaagtc
    10141 aaggcccaag atgaaggaga gggctacacc tggtaagaca gcatgtgaaa tggcatctcc
    10201 catgagtgac atcccgcgta gaataatgaa acatcccaca gctccagcta caatcccgac
    10261 gacaatagct gttatcaagg cattttgtag gaaatggaat ttttgcaatc catcgataaa
    10321 ttctgcaatc ataggtcacc tccattgaaa aagagttgat taccgtaagc ttcttttaga
    10381 ttggtttcgg taaaagtttc ttttgttgga ccaaaggcaa tcacttctcg attgacaagt
    10441 aagacttgat cgaagtagtg gggaatcttg ctgaggtcgt ggtgaacgat gagaaccgtc
    10501 ttcccagctt ttttcaaatc tctcagcgta ttcatgatga tttcctcact gacagagtca
    10561 atcccagcaa agggttcatc caagaggata tagtcggctt cctgcaccaa acatctggca
    10621 atcaaaaccc gctggaattg acctccagac agttgactaa tttgacgttc agcgtagtca
    10681 gctaggccga cgatttcaag ggcctcttgc actttcttcc aatgtttagc ctttaaactt
    10741 cgaaagagag gaatagaggg aaatagtcct aacgagacgc attccttgac cttgatggga
    10801 aagttgtagt cgatattgat tttttgttcg acataggcaa ttcggtgtaa ggatttttta
    10861 acttccttgt catcgagaaa tgcctgacct tgatgtggga taattcccag catacctttt
    10921 aatagtgttg atttcccagc gccgtttgga ccaatgatgc cggtaattgt tggtccatgg
    10981 agcactagtg aaatatcctt aagtgccaac gtttctttgt aggagacact gaggttttcg
    11041 atacgtatca taaacttgta ttcctcctgt ctcttaatat acattaaaaa aaaaattaag
    11101 tcaagttaat ttttgaaaaa attaaaataa taactgaaa
//
LOCUS       DAVKJU010000062        10618 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVKJU010000062 DAVKJU010000000
VERSION     DAVKJU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.062"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(117..1541)
                     /locus_tag="VW191_001663"
     CDS             complement(117..1541)
                     /locus_tag="VW191_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU9624406.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVTWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW191_001664"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="VW191_001664"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEU9624407.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW191_001665"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="VW191_001665"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU9624408.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSISKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="VW191_001666"
     CDS             complement(6528..7706)
                     /locus_tag="VW191_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU9624409.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFFRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="VW191_001667"
     CDS             complement(7786..8649)
                     /locus_tag="VW191_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEU9624410.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     VYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="VW191_001668"
     CDS             complement(8652..10553)
                     /locus_tag="VW191_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEU9624411.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIASLEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQNQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3184 a         2345 c         1901 g         3188 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaattcct
      181 tgcactccag ctccagaacc tttgacacca aggaatggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgtcactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag gagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cccgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaataggag ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttcaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaatcttg atttttcaaa gtatactcaa taaaaattaa aaaaatccac
     1681 tacgtcaagg cgaggctaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctattcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aagtgttcaa
     1921 aaactttgat aggaggtctt gttttgtgaa aatatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt actaatactc
     2041 ataactggga atttgtctgt gtaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaactac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggcag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtatagct
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatgt gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagtc ttacctacca aatcttcttc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgcact tctgtcgctg taggtgacca gagagaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaagga ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaat actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactagcc acttcctctg aatctgcctg
     4861 atacagattt tcaattgaat ggaaatattg aagtaaaagc tgactagcct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 agcagggaac ttgtaggcta ctgcccactt tggagcctta acggtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gtagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcgcttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctcttccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat atggagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgaaaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgcatggcc tcttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatacctt tgtcaataat
     7981 cttctctgta taaacctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaatcaacac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taactcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa aggggagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatc tttacacctg ctctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggttt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgcttt cttctcaaca tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa aaagcaaggt accatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaagttatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggct ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc acctttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc attgggacca
     9481 acgatagcaa cagcattcat cttacgaaga tctaggttaa tcggttgtga caagacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttggtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agaagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtagcta gcttttgctc cttcaactcg acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat gcttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataatgag ggcaccgcta tagtttacca agtaattctc tagccaggcg
     9961 atagtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tggcaagtgc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattc tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgtctc
    10261 tccgtccgac gcaaatcatt aaagacatga agcatttcat cgtagatggt attttcagac
    10321 tcaaaacggc tatcttgggc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccgctagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat cataagagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag tcttttagta caactttt
//
LOCUS       DAVKJU010000063        10421 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVKJU010000063 DAVKJU010000000
VERSION     DAVKJU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10421
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.063"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            2..835
                     /locus_tag="VW191_001669"
     CDS             2..835
                     /locus_tag="VW191_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624412.1"
                     /translation="MIEVSHLSKSFGDKIALNNISFTVKEGQIFGFLGPSGSGKTTTI
                     NILTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMSLYNNLLFYSKFY
                     NISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPAVLFLDEPTSGLD
                     PTTSRTIHELILELKTAGTTIFLTTHDMNEASLLCDYVALLNKGKLVEQGAPSELIQR
                     YNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLEDIFITLTGGKLN
                     A"
     gene            828..1541
                     /locus_tag="VW191_001670"
     CDS             828..1541
                     /locus_tag="VW191_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9624413.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFCKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNETLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(1863..1955)
                     /locus_tag="VW191_001671"
                     /pseudo
     CDS             complement(1863..1955)
                     /locus_tag="VW191_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2276..2977
                     /gene="pyrF"
                     /locus_tag="VW191_001672"
     CDS             2276..2977
                     /gene="pyrF"
                     /locus_tag="VW191_001672"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU9624414.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QLIKQATNPDFICLTPGIRPQGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            3011..3643
                     /locus_tag="VW191_001673"
     CDS             3011..3643
                     /locus_tag="VW191_001673"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU9624415.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            3823..4226
                     /locus_tag="VW191_001674"
                     /pseudo
     CDS             3823..4226
                     /locus_tag="VW191_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4322..5086
                     /locus_tag="VW191_001675"
     CDS             4322..5086
                     /locus_tag="VW191_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624416.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMVVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            5086..5838
                     /locus_tag="VW191_001676"
     CDS             5086..5838
                     /locus_tag="VW191_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624417.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            5848..6546
                     /locus_tag="VW191_001677"
     CDS             5848..6546
                     /locus_tag="VW191_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624418.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            6543..7190
                     /locus_tag="VW191_001678"
     CDS             6543..7190
                     /locus_tag="VW191_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624419.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(7270..8134)
                     /locus_tag="VW191_001679"
                     /pseudo
     CDS             complement(7270..8134)
                     /locus_tag="VW191_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(8146..8904)
                     /locus_tag="VW191_001680"
     CDS             complement(8146..8904)
                     /locus_tag="VW191_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEU9624420.1"
                     /translation="MSETILEIKELKKSFGDNPILQGLSLEIKKGEVVVILGPSGCGK
                     STLLRCLNGLESIQGGDILLDGQSIVENKKDFHLVRQKIGMVFQSYELFPHLDVLQNL
                     ILGPIKAQGRDKKEVTEEALQLLERVGLLDKQHSFARQLSGGQKQRVAIVRALLMHPE
                     IILFDEVTASLDPEMVREVLELINDLAQEGRTMILVTHEMQFAQAIADQIIFLDQGKI
                     AEEGTAQAFFTNPQTKRAQEFLNVFDFSQFGSYL"
     gene            complement(8905..9192)
                     /locus_tag="VW191_001681"
     CDS             complement(8905..9192)
                     /locus_tag="VW191_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9624421.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(9202..9582)
                     /locus_tag="VW191_001682"
     CDS             complement(9202..9582)
                     /locus_tag="VW191_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU9624422.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(9542..10222)
                     /locus_tag="VW191_001683"
     CDS             complement(9542..10222)
                     /locus_tag="VW191_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEU9624423.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         3125 a         1732 c         2142 g         3422 t
ORIGIN      
        1 tatgattgaa gttagccatt tatcaaaaag ttttggtgat aaaatagctt taaataatat
       61 aagcttcact gttaaagaag gtcagatttt tggattttta ggaccatctg gttctggaaa
      121 aaccacaacg attaatattc tgactgggca gttccttgcc gataaaggac aatctattat
      181 tttgggacaa aaatctcaaa atttaacaag cggtgaatta aagagaattg gattggttag
      241 cgatacaagt ggattttatg agaaaatgtc tctgtataac aatcttcttt tttatagtaa
      301 attttataat attagtaaat cacgtgttga taatttgtta aagcgagtag gattatatga
      361 tagtcgcaag atggtagcag gaaaattatc cactggaatg aggcaacgaa tgcttttagc
      421 acgagctctt atcaacaaac ccgctgtact ctttctggat gaaccgacct caggtctaga
      481 tcccacaact tctcgaacaa ttcatgagtt aattttagaa ttgaaaacag cagggacaac
      541 gatttttcta acgactcatg atatgaatga agcaagtctt ttatgtgatt atgttgcctt
      601 attaaataaa gggaaattag ttgagcaagg agctccttct gaactcattc aaagatataa
      661 taaagataaa aagattaagg ttacagatta taatgggaat cagataactt ttgattttac
      721 atcactagaa caggtatctc aggctgatct ggaaaatatt ttttcaattc attcatgtga
      781 gcctacttta gaagatattt ttatcacatt aacaggagga aagctaaatg cttaaacggt
      841 ttctggcttt ggtatggttg cgttgtcaaa ttatcctttc caataagagt attttattgc
      901 aagttttagt gccttttgct ttcacatatt tttgtaaata tcttatggaa acacagggga
      961 aggtcaacga tcaacaggca ttagttcttt tgatgatgtg tttacctttt tctttggctt
     1021 tggctgttgg aagtcctata actattatct tgtctgaaga aaaagaaaag tacaatttac
     1081 aaactcttct gttgagtggt gttaaaggat ccgaatacat tttatcaacg atgtttcttc
     1141 cttttttgct aacttttgtg attatgggaa ctactcctct tattttagga gttacaattg
     1201 tacatacttt taattatatt acaatcgttc ttctaacctc tttatccatc attttattct
     1261 atttattgat aggtttaacc gcgaagagcc aagtagtagc tcaggttatc agtcttcctg
     1321 ctatgatttt agttgctttc ttaccgatgc tatctggttt ggataagaca gttgcgaaga
     1381 taacagatta tagttttatg ggactgttta ctaagttttt cacaaaatgg gagggatttt
     1441 catggaatga aactctaatt cctaatctaa cactacttat ttggattgtt cttctattaa
     1501 ctttaattac gataactatt aggaaaaaga aaatttctta attgagttat tttaatgatt
     1561 ataaacacaa gtgggaagga aaaatgaact gatctttttg acagcaattc tacagaatag
     1621 tcttattgct atattttgat ttgagtgtac gaaaaaagaa aaataacaat agtgctcata
     1681 ctaattgcag aagttttggg tgataagata actgataaat tgcaataaaa aatgcaacat
     1741 ttttaaatct cctctataag tgcttcaaaa cctgtcttgt aatccaagta tttttgggaa
     1801 cggtgattaa taagctagca aagcaccatt aaggattttt tcggtaattg ttgccaaatc
     1861 ggtttaagaa aatactcacg aagaagtcca ttcgcattct cattacttcc cctttgccaa
     1921 gatgaatagg catccgcaaa ataaaacaga attcccattt gttcaattaa agggtaacaa
     1981 gcaaactctt tttctctgtc ctcagtgaaa gtctttaact attcttttgg aaagagtctt
     2041 gtgaggtgtt caatagcagt caacacatac gtgaccatgt ttttacttga caagtgctag
     2101 tagaaataat agaataatag aaaaccttta aagcagtcca gagaggcagc taaggttaga
     2161 cggtgaaagg gtggagacta cccatttttc gtggaacctt gctgttggca ggttcctttt
     2221 ttcgtggctt ctgttggcca gactctctca ctagtaaagg taaaaggaga aacctatgcg
     2281 agaacatcgt ccaatcattg ctcttgattt tcctagtttt gaggcggtca aggaattttt
     2341 agctcttttc ccagcagaag aaagccttta tctcaaggta gggatggagc tttattacgc
     2401 agcggggcct gagattgtgt cctacttaaa aggtttgggt catagtgtct ttttggatct
     2461 caaacttcat gacattccta atacagtcaa gtcagccatg aaggtcttgt ctcagcttgg
     2521 tgtcgatatg accaatgttc atgcggctgg tggtgtagag atgatgaagg ctgcgcgtga
     2581 aggtcttgga agtcaagcaa aattgatcgc tgtaacgcag ctgacatcaa catcagaagc
     2641 ccagatgcag gagtttcaaa atatccaaac aagcctacaa gagtctgtga tccactatgc
     2701 caagaagaca gctgaagcag gcttggatgg tgtcgtttgc tcggcacagg aagttcaact
     2761 tatcaagcag gccaccaatc cagattttat ctgtctgaca ccagggattc gcccacaggg
     2821 agctgctgtt ggagaccaaa aacgcgtcat gacgccagca gatgcttatc aaattggtag
     2881 tgactatatc gtagtgggac gtcccattac ccaagctgag gatcctgttg cagcttatca
     2941 tgccatcaag gatgaatgga cacaggactg gaattaaaga actagattag aaaaataaaa
     3001 ggagaatacc atgacacttg ctaaagatat cgctagccac ctcttgaaaa tccaagccgt
     3061 ttacctcaaa ccagaggaac ccttcacttg ggcgtctggt atcaagtcac cgatttacac
     3121 tgataatcgt gtgacactag cctatccaga aactcgtacc ctaattgaaa atggttttgt
     3181 ggaagctatc aaagaagcct ttcctgaagt agaagtgatt gcaggaactg caacagcagg
     3241 gattccacac ggagccatta ttgctgataa gatggacttg ccttttgcct acatccgtag
     3301 caaaccaaaa gaccacggag ctggtaatca aatcgaaggt cgcgtagctc aaggtcaaaa
     3361 aatggtagtg gttgaagacc ttatttcaac gggtggttca gttcttgaag ctgtagcagc
     3421 agccaagcga gaaggagcag atgtacttgg agttgtagcg attttcagct accaattgcc
     3481 aaaagcagat aagaactttg cagatgctgg tgttaaactt gtgacgcttt caaactatag
     3541 cgagcttatc catctagccc aagaagaagg ttacatcacg ccagagggcc ttgatcttct
     3601 aaaacgcttt aaagaagacc aagaaaattg gcaagaaggt taggtcagta agataaagag
     3661 agacgaggct accgagtctc ttttaccatt ttatttaaaa tatgataata ttttaaataa
     3721 aataattgac aacgctttct tttattgata taatgaaaat gtaatatgta tcaaattgat
     3781 caatcaacta tcttgtttat cacttcttaa gaggacgatg tcatgaaaag aagaaaaata
     3841 tattcctttt ttgcgtagtt acaattgcat ctagtttgtt tctccctaca gttttagcta
     3901 gacagattgg aaattatgat ttaacgttgc cgcgttgggg ttcggataca actagtgagc
     3961 ttgagaaaga aaacgcctct gctggaatta ataatagtga cagcactggt ggcggtaaaa
     4021 ggttaaatac ctctattcgt agcgcctata gtgggtcaga tattaccccg gtatattcat
     4081 tggggtctgg ctctaggatt gtcatgtact ataatggagg tggtgacaat tatattggtt
     4141 ctggtactag attagctatg gcgccacaat ttggaaatca tgtaagaatt catacttcag
     4201 gttcttggag tccagattct tattaactta cttgtcagag taagccttaa agatggttga
     4261 ttgtgggtgt agcatgaaaa aagaatgcta caccctattt ttattataag gaggagtaag
     4321 gatggaattt ttcatttgta atcttgtacg agtcgttcaa tcacctcggt tttatatgtc
     4381 tttatttttg acccttcttt gcatgagttt aggaaatttc cttgctttca atggtattta
     4441 taaaattgaa ggtttatcga ttttttttgc cgcttcttct attcgaggat tttcaccgat
     4501 tagcctagta gctgcactta tctgtacact gccctattct agtcagataa tagaggatgc
     4561 tgagagtcat tttctaacag cacaattgtg ccgaatttct aaaaagaggt atctagctat
     4621 tgtaggtagt actgcaatta tttcttcttt tctagtcttt tttctcccct atttattatt
     4681 attaggaatt aatcttttag tgactcctta tcaggaaatt tatattggag attatagtgg
     4741 tgccttaaaa gaattatttg attctaatca gttgctctat agtcttgtga cgactctctg
     4801 gtatggagtt tggggcgctg tgttctctat ttttggacta gctagtgctt tgctagtgaa
     4861 gaaaaaatta ggagctattt tcatcccagt tgcctatatg gtggttggtg gtattttatg
     4921 ggctatttta gggctatctt acttagaacc tgtgacaacg ctagctttgg gatatcagaa
     4981 agatatcagt ctttccttag ttagtgggca tcttgctttt attttatttg ttagttgttt
     5041 ggttgtttat ggtacatttt ttctacattc agaggactat gtataatgaa acaatttgtt
     5101 caattttata aaaaagattt cttagcagta ttggtttatt ttatattact gctatcctgt
     5161 gttttatcta gtacagtata tttattgcgc tgtcgccaat attcaatcca tccaaatgta
     5221 ttagaatgga ttttagtttt acttcaagat atgacgactg gagtatattg ctttccgttc
     5281 acatatatat tgttcttttt ttatttgatg aataactatt ttaataggtt ggagtgtcgc
     5341 attcgtctga aatcaattaa gcactttacc agttttagtt tcaaattagc agctcttagt
     5401 acggggattt ggacggcgac tttattttta ttgatttttc taattgcatt tagtaatggt
     5461 tttagcttct ctttggagat aaaggaggtt gattttttaa gagaatttta tggtataagt
     5521 attgcaaaca atgctagttt ctttatagga ttttttttct cttatatagc atactatttc
     5581 tttttatcct tacttactat tagcagtttt tcttggttta aaaaatcaaa catgagctta
     5641 gtatttctgt ttactttttt atttgtagaa tccttattct ggatttatca gttgaacaat
     5701 gggataattg gattattgcc aatttttcag tatatggtaa attccaatcc gtatgcattg
     5761 atttattggc ttacattact atctatcata attccattga ctgtattttc tgttcataga
     5821 aactggagga gagtgtaaaa gttggaaatg ggaaagttaa gtagtcacat gtggaggttg
     5881 aatcagataa tctataccaa gtacttttgg ggttatgttc ttttttggat attgatttgt
     5941 ttaggattat ggtattggtt agaaggaaat gatagacttg ttatagaaat tttaaaaggg
     6001 cctaatctga gtcaaaactc ttttttagtc ttatctatat ggttgcttca ttggtttatt
     6061 attcatacat tttttctagc agttgtatat cgtagaagag catccgattt ctttatggaa
     6121 gtgattcgat tttcttctat taagctctgg attaggtatc agatttggac ctgttttctt
     6181 tatggactca ttttaatcat ggtaaaagtt ctagtgattc aatttatgtt acagttagca
     6241 aactgggata taggagtttt gtttatagtt gattctttga atgcttgtgt gctatccttg
     6301 ctttgcttta tgttatacgc actaggagcg aatgtacaaa tgaactttgc ttgcgttagt
     6361 ttctttttac tcatgattgt gtttggtggt ctatttgtag gaaatcgaac taattatcta
     6421 ttttatattt tgaatcgagg gaatggagat ataggacgag atttatttct tcagttgcta
     6481 tttttagtct ttctttttaa aagtattttc tatttcactc gtcaaaagag gaggtttata
     6541 gaatgaatga aataatcata ttaaaaaata ttgagttgaa attaaaaaaa acatgtgttt
     6601 ttcaaaacct taattttagt tgtaaacagg gggaaattat aggaattact ggtgcgaatg
     6661 gctcagggaa aagtgtattg tttaaattaa tagctggttt atatagtccg tcttatggag
     6721 aagtgttaat caatggggaa aatattgttc ctgagagaaa aattccagct aatttgggag
     6781 ctttgattga agaacctggt tttataaatt attatagtgg ctttaagaat ttacaatatt
     6841 tggcaagcat acgaggagta gttggtaatc aggaaatcaa tgatacactg aaaatagttg
     6901 gtctatatga gcaaaaagac cagaaagtta aaacttattc gctaggtatg aggaaaaagc
     6961 tagggattgc tcaagcaatt atggagaatc cctctattct tttactagat gaacctatga
     7021 atgccttgga taaatcaagt gtagaaaata tgagaacatt gtttagaaag ctctctagtg
     7081 aaaaagggac aacaattttg attgctagtc acagtgaaga ggatattcgt atcttatgtg
     7141 ataaagtata tgcaatagaa gataaagtat gtacactgtg ttcagattga acatcttaga
     7201 tagactcagg atgttatagg taaaatctta aaaagggatt ggagcttaaa atccagtccc
     7261 ttttaagagt taatggccac cttcaacaac caggtcatct gctttagcag cgtcaccgta
     7321 ggttgggtga agtgtttttt cataggcctt gtggaagaag ttttccttgc taatttttca
     7381 atatctttat tgatgaagtc tagcaattct tggttgcctt tttgaactgc tgccgcaatg
     7441 gtatcgggat caccgaggga agtaattcct acttcaaatc ctttattttc aagcgcccaa
     7501 gctagaactt ccgtattgtc agttgaaaag gcatctccac gtccgtcaag aagagcttgg
     7561 taagagtcac tgtattggtc gtatttttgg agtttgattt ctggatgatt cttttcaaaa
     7621 taagtctcag cagtcgttcc ttttgtgaca attaaggttt taccttcaag ttgtttgacg
     7681 tctgtaatga gaccagtctt aggtgatacg acacccagag aaactttcat atatggaagg
     7741 gcaaaatcaa cttgtttctt acgttcgtca gttactgtaa agttagcaag agtaatatct
     7801 accttgtttg aaatcaagta ttccgcacgg ttgtcagcat cgactgaaat gtatttaacc
     7861 ttgacaccaa ggtcttgagc tagttggttc cctagttcaa tatcgtagcg tagccttggt
     7921 aagaaccatc attgtcaacg tagccaaacg gttttttatc tccaaacacg gcgattcgca
     7981 gttcaccgct ttttttgatt tcatcgatag tgcgagcttt ggcagttgtt tttccagagg
     8041 atgaaccagc gtttccacct gagctacaag cagtgataaa gataagggca aaggcaagtg
     8101 ctaaaacagt taagagtggt ttgaatagtt tcataagaat ctcctttata gatatgagcc
     8161 gaattggcta aagtcaaaga cgtttaaaaa ttcctgggct cgtttggttt gcggattggt
     8221 aaagaaggct tgagctgttc cttcttcagc gattttccct tggtcgagga agataatctg
     8281 gtcagcaatg gcttgggcaa actgcatttc gtgggttact aaaatcatgg tacggccttc
     8341 ttgggccaaa tcattgataa gttccagcac ctcacgcacc atttctggat ccagcgaagc
     8401 agtcacctcg tcaaaaagga tgatttctgg atgcattagg agggcacgga caattgcaac
     8461 acgttgcttc tgtccaccag ataattgacg ggcaaagcta tgttgtttat ccagcaaacc
     8521 gacacgctct agtaattgca aagcttcttc cgttacttct ttcttgtccc ttccttgagc
     8581 tttgataggg cctaggatga ggttttgtaa gacatccaga tggggaaaga gttcataact
     8641 ttgaaagacc atgccaatct tttggcgaac taggtgaaaa tcttttttat tttcaacgat
     8701 agactgacca tccagaagaa tatctccacc ttgaatactt tctaagccgt tgaggcaacg
     8761 aaggagggta cttttcccac aaccagatgg ccctaggatg acaacaactt cccctttttt
     8821 gatttctaga gaaagtcctt ggaggatggg attgtctccg aaggattttt ttagttcctt
     8881 gatttctaag atagtttcag acatttagtt tctccaatgt ttttctaagt gagtggatag
     8941 tttggaaata gggtagcaaa ctgcgaaata taagactaga atggttccat aaatccaaaa
     9001 tgaagcagtt gggatggtca ggcgattgct atcgatgatt tgttgtccaa ctttggtcac
     9061 ttccacaacc ccaatcaaaa caactaatga agtggtttta atcatccgag tgacaagatt
     9121 gatagcctgc ggtagcagtc ttctcaagac ttgtgggatg atgatgtggt agtaaagttg
     9181 aacattagtc aagtcgagtg cctatccact ttcaaactga tgtttaggga gagaagtgat
     9241 agctccacgt accaagtctc ccatttcagc tgttccccag agggtaaaaa tgataatagc
     9301 tgaagtctca cctgagatat tgatattaaa gtttcgagcc aagccaaagt aaacgatgaa
     9361 gagtagcacc agctggggca tgatacggat aaattccaga tacaatcgtg ttaaaaatcg
     9421 tatgattcta gaatgggagg tcatgatgat tcccatgact gttccgaaca tcatggataa
     9481 gaggacagac aggatggata tcccaatcgt aacgcccaac ccctgtaaga ttctcaggag
     9541 attatttcct tgaaagagta cttggattcc cgaatcctgc atggcgggtc ctcctttcta
     9601 tccagctaaa gaccagtgag atgggtagca gcatgatcag ataagcaact accaacatag
     9661 ctagcgcaat gtctgtctca tagtagagac caatcaaatc cttggcgacg tacatgaggt
     9721 cggccaaagc cactgctgag aaaacagagg tttccttgat aaggaaaatg acattggcac
     9781 taaaggaggg gagtgccacc gctgttgctt gcggaagaac cacatagcga aagacctgta
     9841 gaggtgtcag accaatagcg aggccaatct cctgctgggt ttgactgatg gcttccagcc
     9901 cacttcggaa agattctgcc atataggagc ctcctaaaaa gacaagccca agcgttgcac
     9961 agacttctga agataggaca atcccgattc ggggaagacc gaagtagaga aagaagagtt
    10021 gaatcaaaag gggcgtatta cgtgacaatt caatgtaggc tgtcgctact tgcgccaaaa
    10081 cagggattcg ataatgtcgg atgatactaa cgattaaacc gagcagaaag gatcccaaaa
    10141 ttccccaaac tgcaatatgc aaggtcagaa agaatgcctt ttgatatagt ggtagatatt
    10201 gttcaacaat ggaccaatcc aaaaatagaa cctcccatct agaaataata cagttattgt
    10261 agcacttaaa atcttctttg gataatatct attttttatt gccgttataa ggatttttta
    10321 ccatagacat aaaatttctg aaatttccaa acaaaatatt tgaaaagttt tgaaaaagag
    10381 ttaagatatt tttgtaatat acaaagtgaa cgcttactta t
//
LOCUS       DAVKJU010000064        10376 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVKJU010000064 DAVKJU010000000
VERSION     DAVKJU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.064"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            38..514
                     /locus_tag="VW191_001684"
     CDS             38..514
                     /locus_tag="VW191_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9624424.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            552..854
                     /locus_tag="VW191_001685"
     CDS             552..854
                     /locus_tag="VW191_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9624425.1"
                     /translation="MIKILAACGAGVNSSYQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIRLPAKQNMV"
     gene            862..1665
                     /locus_tag="VW191_001686"
                     /pseudo
     CDS             862..1665
                     /locus_tag="VW191_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1714..2178
                     /locus_tag="VW191_001687"
     CDS             1714..2178
                     /locus_tag="VW191_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEU9624426.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2335..2760
                     /gene="lacA"
                     /locus_tag="VW191_001688"
     CDS             2335..2760
                     /gene="lacA"
                     /locus_tag="VW191_001688"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEU9624427.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2791..3306
                     /gene="lacB"
                     /locus_tag="VW191_001689"
     CDS             2791..3306
                     /gene="lacB"
                     /locus_tag="VW191_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEU9624428.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3317..4246
                     /locus_tag="VW191_001690"
     CDS             3317..4246
                     /locus_tag="VW191_001690"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEU9624429.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4248..5228
                     /gene="lacD"
                     /locus_tag="VW191_001691"
     CDS             4248..5228
                     /gene="lacD"
                     /locus_tag="VW191_001691"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEU9624430.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGETAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5461..6297
                     /locus_tag="VW191_001692"
     CDS             5461..6297
                     /locus_tag="VW191_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEU9624431.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6333..6650
                     /locus_tag="VW191_001693"
     CDS             6333..6650
                     /locus_tag="VW191_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9624432.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6650..8341
                     /locus_tag="VW191_001694"
     CDS             6650..8341
                     /locus_tag="VW191_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEU9624433.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKVG
                     VEAAQNTITKETNVLVLCAGGGTSGLLSNALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8556..9962
                     /gene="lacG"
                     /locus_tag="VW191_001695"
     CDS             8556..9962
                     /gene="lacG"
                     /locus_tag="VW191_001695"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEU9624434.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGFGYKDEFVNNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            10005..10214
                     /locus_tag="VW191_001696"
     CDS             10005..10214
                     /locus_tag="VW191_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEU9624435.1"
                     /translation="MFKDFIQSIYEKVYIINFEKYSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDDLEEFKNLFLNFINPEE"
BASE COUNT         3300 a         1848 c         2136 g         3092 t
ORIGIN      
        1 ttatagcata aagttaatgg aaagaaggga aaaccttatg gtattagatt atttctttga
       61 caaaaacctt gtgttttgct tagaagcgga taatcaagaa caactctttg atcaggtagc
      121 aagcttgttg gaagaacgag aaattgtcac tccaacttat cgtgaagcct tgatcacgcg
      181 tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaaggact tgccaaatgt
      241 agcgattcct cacacagata ttgttcataa tttggctgaa aaagttgtgg tcgttcgatt
      301 agagaaaccg gtgactttcc ataatatgat tgcgccagat aaggaagtag aagtatcctt
      361 gctcttcttt atcattaata attcaagttc aagtcaaacg aacattcttg ctcagttaat
      421 ggacttcttc acaggaaatg gtcatcttga agacctatca aaaatttctg aaccagaagc
      481 cctttatgct tacattgctg aagcaaccgc ttaatcttgt ctattaaaaa ataaaatcgg
      541 aggaaatcca aatgattaaa attcttgctg cctgcggtgc aggtgttaac tcaagttacc
      601 aaattaaaag tgctctagaa gaagaacttt caaaccgtgg ttatgatgtt cactgtgatg
      661 cagtcatggt gaaagatgta aacgaagacc ttatgaaagg ttacgatatc tttacaccaa
      721 tcgctgcaac agatcttggt tttgaaccag gtatcccagt tatcgaagct gggccaatct
      781 tattccgtat accagcaatg agcgctccag tatttgacaa tattagactc cctgcgaaac
      841 aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac tgatgcacga
      901 tttaagcgtc ttgttggtgt tcagcgtacc acttttgaag agatgttagc tgtattaaaa
      961 acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac
     1021 cttcttatgg ccactcttca atatgtgcga gaatatcgaa cttatgaaga aattgcggct
     1081 gattttggtg ttcacgaaag caacttaatc cgtcggagcc aatgggttga agtaactctt
     1141 gttcaaagtg gttttacgat ttcaagaact cctctcagtt ctgaggacac ggtaatgatt
     1201 gatgcgacgg aagtacaaat caatcgccct aaaaaaagaa ttagcgaatt attctggtaa
     1261 aaaaaatgcc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag aattgtttct
     1321 ttggatatcg ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag tcgcagaaat
     1381 atcggacaag ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat
     1441 cctcaagcac aaactccacg taaatccagc aaactcaagc cactaacagt tgaagataaa
     1501 gcctataacc atgcgctatc caaggagaga agcaaggtcg agaacatctt tgccaaagta
     1561 aaaacgttta aaatgttttc aacaacctat cgaaatcatc gtaaacgctt cggattacga
     1621 atgaatttga ttgctggtat tatcaatcat aaactagaat tctagttttg caggaagtct
     1681 aataaagaaa ctagcaaggt caggccttac tttattggtg gacatccagg atttaattgt
     1741 cttctacttg atgatgaagt ctatgaagat tattatctag agtttgagaa agaagagatt
     1801 tgctctgttc cacgtccttt cccagaaaca ggtatgttgg atttccaaga tagaagtcca
     1861 tggctagagg gacaaaaaga aatagatctt agttatgatc ttttcagcac agatgcagtg
     1921 actttggatg aattgcaatc tcgaacaatt gcccttcgtt ctcttaaaca tgataaggga
     1981 ttgaaagtgc attttgcaga atttccaaac ctcatcatct ggtcaacttt gaacaaggga
     2041 cctttcatta cctttgaacc atggtctggc ttgtcaacat tccttgaaga aggagatcat
     2101 ttagaagata agaagaatgt ttgtctctta gaagccaatc aggttgaaga attagggttt
     2161 gagatagaag ttttataaaa aagtaaaaaa taaataacaa aaacaaacac aaactgttga
     2221 cttcttcaaa caatagtagt atattatgtt tgtgaggact aattgactgt tttttaaaga
     2281 cagccaatac actgctggaa tccagcatag aaaaaataaa aaggagtata caatatgtct
     2341 attgttatcg gtgcagatgc tgcaggtttg agattgaaag aagttgtgaa agacttctta
     2401 gaaaaagaaa acttccacct tgtggatgtt acagctgaag gtcaagactt tgttgatgtg
     2461 actctcgctg ttgctgcaga agtaaacaaa gaagaacaaa accttggtat cgtgattgat
     2521 gcttatggag ctggtccatt tatggttgca actaagatca aaggaatggt tgctgcagaa
     2581 gtatctgacg aacgttcagc ttatatgact cgtggccaca acaactcacg tatgatcact
     2641 atgggggcac aacttgttgg tgatgaattg gctaaaaaca tcgctaaagg atttgttaat
     2701 ggtaaatacg acggtggtcg tcaccaaatt agggtcgaca tgttgaacaa aatgggctaa
     2761 ttagattata gtaagaaagg taagttaaaa atgagaattg caattggatg tgaccacatc
     2821 gtaacagatg aaaaaatggc ggtttcagaa tttttgaaat caaaaggata tgaagtcatt
     2881 gactttggta cctatgacca tacacgtact cactacccaa tctttggtaa aaaagtaggg
     2941 gaagcagtaa ctagtggtca agctgatctc ggggtatgta tctgtggtac tggtgttggt
     3001 atcaacaacg ctgtaaataa agttccaggt attcgttctg ccttggttcg tgatatgaca
     3061 acagcccttt atgctaaaga acaattgaac gccaacgtta tcggttttgg tggtaagatt
     3121 actggtgaat tgcttatgtg tgatatcatc gaagctttca ttcatgctga atacaaacca
     3181 tctgaagaaa acaaaaaatt gattgcgaaa attgaacacc ttgaaagtca caatgctcaa
     3241 caaacagacg caaacttctt cactgaattc cttgaaaaat gggatcgcgg agaataccac
     3301 gactaagagg taacctatga ttttaacagt tacaatgaac ccatccattg atatttccta
     3361 tcccttggat gagttgaaga ttgatactgt caatcgtgtg gtggatgtaa ccaaaacggc
     3421 tggtggtaag ggactcaatg ttacccgagt actttcagaa tttggcgatt ctgttcttgc
     3481 tactggttta gtgggtggca aacttggtga gtttttggtt gaacatatcg ataatcaagt
     3541 aaagaaagat ttcttctcaa ttcagggaga aactcgtaac tgtatcgcta ttctccacgg
     3601 agacaaccaa acagaagttc ttgaaaaagg tcctgaagta ttggaacagg aaggtcaaga
     3661 ctttttggaa catttcaaaa aactcttgga gtcagttgaa gtagtggcca tctcaggtag
     3721 tctgccagct ggccttccag ttgattacta tgcgagcttg gtagaacttg ctaatcaagc
     3781 tggcaagcct gtagtcttgg actgctcagg tgcagcactt caggctgttc ttgaatcacc
     3841 ccataaacca acagtcatca aaccaaataa tgaagaattg tctcagcttc ttggaagaga
     3901 agtttctgag gatttggatg aattaaaaga agtacttcaa gaacctttgt ttgcagggat
     3961 tgaatggatt atcgtttcac ttggtgccaa cggtactttt gccaaacatg gtgacacttt
     4021 ctacaaggta gatattccta gaattcaggt ggtaaatcct gttggatctg gagactctac
     4081 tgtggcagga atttcttcag gacttcttca caaagaatcg gatgcagaat tactcatcaa
     4141 ggcaaatgtc cttggtatgc tcaatgctca agaaaaaatg actggtcatg tcaacatggc
     4201 caactatcaa gttctatatg atcaattaat agtaaaagag gtataaaatg gctttaacag
     4261 aacaaaaacg tgtacgctta gaaaaacttt ctgatgaaaa tggtatcatc tcagctcttg
     4321 catttgacca acgtggtgct ttgaaacgcc tcatggctca acaccaaaca gaagaaccaa
     4381 ctgtggccca aatggaagaa cttaaagtct tggtagcaga tgaattgact aaatacgctt
     4441 catctatgct tcttgaccct gagtatggac ttccagcaac taaagctctt gatgaaaaag
     4501 ctggtcttct ccttgcttat gaaaaaacag gttatgacac aacaagcaca aaacgcttgc
     4561 cagactgctt ggatgtctgg tctgcaaaac gtattaaaga agaaggtgca gatgcagtta
     4621 aattcttgct ttactatgat gtagatagct cagacgaact caatcaagaa aaacaagcct
     4681 acatcgaacg catcggttct gagtgtgtgg ctgaagatat cccattcttc cttgaaattc
     4741 ttgcttacga tgaaaaaatt gcggatgcag gttctgtaga atacgcaaaa gtaaaaccac
     4801 acaaagttat cggcgctatg aaagtctttt cagacccacg ctttaacatt gatgtcttga
     4861 aagttgaagt tcctgttaac attaaatatg ttgaaggctt cgctgaaggt gaagtagttt
     4921 atacacgtga agaagcagca gccttcttca aagcgcaaga tgaagcaacg aacttgccat
     4981 acatctactt gagtgctggt gtatcagcta aactcttcca agatactctt gtatttgctc
     5041 atgaatcagg tgcaaacttt aacggagttc tttgtggccg tgcaacatgg gctggatcag
     5101 ttgaagcata catcaaagat ggtgaaacag cagctcgcga atggcttcgc acaactggat
     5161 ttgaaaacat tgatgagctc aacaaagttc ttcaaacaac agcgacttca tggaaagaac
     5221 gtgtgtaaga aagtcctcct agtttaggaa catgaatcta aaaaatttaa aaaaagttgt
     5281 atgtaaaggt ttacaaaata acttacttgt gctatactta aatcacaagt taatacaagg
     5341 tgagtgttac taagtaatat taggcatgat cacaggtgaa ttagaaatca gctgattttc
     5401 tagttcattt gtggtcattt tttgtactca tataccttta agatataaaa ggaggttgac
     5461 atgtatcgaa ttctaaatcc aatgaatcac aatgtctcgc ttgtcagaaa tgataaggga
     5521 gaagaggtga ttgtaattgg taaggggatt gcattcggaa agaagaaggg ggatttgatt
     5581 gctgaaaatc atgttgagaa aatctttcgg atgaagaccg aagagtccag agaaaacttt
     5641 atggctcttc tcaaagatgt tccgcttgat tttatcacag tgacctatga aatcattgat
     5701 aagctatcaa agaaatatca ttatccgatt caagagtatc tctatgtaac cttgacagat
     5761 catatttact gttcttatca agctctaact caaggaaggt acaaggatag taatctgcca
     5821 gatatttctg ctaagtatcc tgtcgctttt caaatcgcaa atgaagcttt tgaaatttac
     5881 cgtcagaagc tagcagatca ttttcctgag gacgaaatta ttcggattgc ttatcatttc
     5941 attaatgctg aaggtgaaaa tgaagtggaa cttgtggagt cgattgataa gaggaaagaa
     6001 attctcagga atgttgaaga agttttaacg gactatgcaa ttcaacgaac taaaaagaat
     6061 aaccatttct atgatcgctt tatgatccat ttgaattatt tcttggatta tttagacaga
     6121 tctagagatg ataaccaatc acttctggat atggaagatc atattaaaca atcctatcca
     6181 aaagccttcg agattggttc caagatctat gatgtgatta cgcaacatac gggtcttgat
     6241 ttgtataaaa gtgaacgagt ttatctagtt ctacatatcc aacgtttatt gtcataaaaa
     6301 tttatttaaa actatataag gagaattcta tcatgaatag agaagaagta acattgttag
     6361 gttttgaaat cgtagcctat gctggcgatg ctcgttcaaa actattggaa gccttgaagg
     6421 ctgctgaagc tggtgatttt gaaaaagcgg acgctctggt agaggaagct ggtagctgta
     6481 ttgcagaggc tcaccacgcg caaacaagtc tattgactaa ggaagcttca ggtgaggact
     6541 tggcttatag tgtaaccatg atgcatggcc aagaccactt aatgacaact atcttgttaa
     6601 aagatttgat gcatcattta attgaactct acaagagagg agttaaataa tgaataaact
     6661 aattgcattt atcgagaaag gaaagccttt ctttgaaaaa ctatctcgta atatctatct
     6721 tcgtgctatt cgtgatggtt tcattgcagg tatgcctgtt attctcttct caagtatctt
     6781 tatcttgatt gcctttgtac caaactcatg gggctttaaa tggtctgatg aagttgtagc
     6841 ctttctgatg aaaccttata gctattctat gggtattctg gctctcttgg tagctggtac
     6901 aacagctaag tcattgactg actcagtaaa ccgtagcatg gaaaaaacca atcaaatcaa
     6961 ctatatgtca acattgttgg cagcaattgt tggtttgttg atgttggcag ctgatcctat
     7021 cgaaagtggt ctagctactg gattcttggg gacaaaaggt ttgctttcag ccttccttgc
     7081 tgccttcgtt actgtagcca tctataaggt ttgtgttaag aacaacgtca ctattcgtat
     7141 gcctgacgaa gttccaccaa atatctcaca agtctttaaa gatgtgattc cattcactct
     7201 atctgttgtt tctctttatg ctcttgactt attagcacgt cattttgttg gttctagtgt
     7261 ggcagaatca atcggtaaat tcttcgcacc actcttctca gcagcagacg gataccttgg
     7321 tattaccatt atctttggtg cctttgcctt cttctggttt gttgggattc atggtccatc
     7381 tatcgttgaa ccagctatcg cagctattac ctatgccaat gccgaagtca acttgaacct
     7441 tctccaacaa ggtatgcacg ctgacaagat tcttacttct ggtacacaaa tgtttatcgt
     7501 taccatgggt ggtactggtg cgacactggt tgttccattc atgttcatgt ggttaacaaa
     7561 atcaaaacgt aaccgtgcaa tcggacgtgc ttcagtagtt cctaccttct tcggtgtaaa
     7621 tgagccaatc ttgtttggtg cacctcttgt tttgaatcca atcttcttca ttccatttat
     7681 ctttgctcca attgcaaacg tatggatttt caaattcttt attgaaactc ttggaatgaa
     7741 ctcattcact gctaatctac catggacaac tccagctcca ctaggtctag ttcttggaac
     7801 taacttccaa gtgctatcat tcattcttgc tgcccttcta atcgtggttg acgttgtcat
     7861 ttactatcca ttccttaagg tctatgatga acaaattctt gaagaagaac gttcaggtaa
     7921 gtctaatgat gaattgaaag aaaaagttgc tgcaaacttc aacactgcaa aagcggatgc
     7981 tattcttgaa aaagtaggtg tagaagcagc acaaaatacc atcactaaag aaacaaatgt
     8041 cctcgttctc tgtgcaggtg gaggaacaag tggtctcctt tcaaatgctc taaacaaggc
     8101 agcagcagaa tacaatgtcc ctgtgaaagc agcagcaggt ggctatggtg ctcaccgtga
     8161 aatgttgcca gagtttgatt tggttatcct tgctcctcaa gttgcttcaa actttgaaga
     8221 tatgaaggca gaaacagata agctcggtat taaacttgct aagacagaag gcgctcaata
     8281 catcaaatta actcgtgatg gaaaaggtgc cctagcattc gtacaagagc aattcgatta
     8341 aggctaggaa ctgtgaaaca gtctcccatc gttacggaaa tcgctatggc gaatttccta
     8401 atcgccttat taattcgtcg gtaaaaagat atcgttttta cctcctcatg tcacaattcg
     8461 gtgacttggt acaagaagtg agatggagaa ggatggctca ctgactcctc tcctctcact
     8521 tttactttat ttaaatcaag aaataggtga aaaaaatgac aaaaacactt ccaaaagact
     8581 ttatttttgg tggcgcaaca gctgcctatc aagcagaagg tgctacacat actgatggta
     8641 aaggaccagt agcgtgggat aaatatcttg aagataacta ctggtacact gcagaaccag
     8701 ccagtgattt ttacaatcga tatccagttg acctcaagct agcagaagag tatggtgtca
     8761 atggcattcg aatttctatt gcctggtcac gtattttccc gactggttat ggtcaagtaa
     8821 atgctaaagg tgttgagttt tatcataatt tatttgcaga gtgtcacaaa cgtcatgttg
     8881 agccctttgt aactcttcat cactttgaca cgccagaagc tctccactca aatggagact
     8941 tcttaaaccg tgaaaatatc gaacattttg tagactacgc tgccttctgt tttgaagaat
     9001 ttccagaagt aaactattgg acaaccttta atgaaattgg accaatcggt gatggtcaat
     9061 atttggttgg gaaattccct ccaggtatcc agtacgacct tgccaaagtc tttcaatcac
     9121 accacaatat gatggtgtct catgcatgcg cggtaaaatt gtacaaagag aaaggctata
     9181 aaggggaaat tggtgttgtt cacgccctgc ctactaaata tcctctagat cctgaaaatc
     9241 cagcagatgt tcgtgcagca gagttggaag atatcatcca caataaattc atcttagacg
     9301 caacttatct aggtcgctat tcagctgaaa ccatggaagg tgtcaaccat atcttatcag
     9361 tcaatggtgg tagtttggat cttcgtgaag aagattttac agcattagaa gctgcaaaag
     9421 acttgaatga tttcctagga atcaactact atatgagtga ctggatggaa gcctttgatg
     9481 gagaaactga aattatccat aatggtaaag gtgaaaaagg aagctctaag tatcaaatca
     9541 aaggtattgg tcgtcgtgta gctcctgact atgtatcacg cacggattgg gattggatta
     9601 tctaccctca aggtttgtat gaccaaatca tgcgtgtgaa gaaagattat cctaactaca
     9661 agaaaattta tatcactgaa aatggtttcg gctataaaga tgagttcgtt aataacactg
     9721 tttacgatga tggtcgtatt gattacgtga aacaacactt ggagatcttg tctgatgcga
     9781 ttgcagatgg agctaatgta aaaggttact tcatttggtc attaatggat gtcttctcat
     9841 ggtcaaacgg ttatgagaaa cgttatggtc tcttctatgt agattttgaa actcaagaac
     9901 gttatcctaa gaaatcagct cactggtaca agaaagtagc ggaaactcag attatagact
     9961 agtagaatta gtcattagat atagaatttt agtgagtcaa aaagatgttc aaagatttta
    10021 tccaatctat ttatgaaaaa gtttatatta taaatttcga aaaatactct caaataccgt
    10081 gtttgacgag tgaagaattg aaaagtcttg gaaaatggta tgtctcgact ggtaaagaat
    10141 ggatttgtca ttcagatgat gatctggaag aatttaaaaa tctattttta aattttatca
    10201 atcctgaaga atagaaaaac gcatagtatc aggtgttgaa acacctgata ctatgcgttt
    10261 tattgtggga agatttactt tttttcttct gaaattgagt tgttacccag gctctttcag
    10321 tttattaagg cttgatgact ttaatgtgtt tagatagctt aaaaaggatt gaatca
//
LOCUS       DAVKJU010000065        10204 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVKJU010000065 DAVKJU010000000
VERSION     DAVKJU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10204
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.065"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..306
                     /locus_tag="VW191_001697"
                     /pseudo
     CDS             1..306
                     /locus_tag="VW191_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            404..616
                     /locus_tag="VW191_001698"
                     /pseudo
     CDS             404..616
                     /locus_tag="VW191_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            516..962
                     /locus_tag="VW191_001699"
     CDS             516..962
                     /locus_tag="VW191_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9624436.1"
                     /translation="MRKHGLISKSTSKGYYQVAIPFMRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VW191_001700"
     CDS             complement(1100..2404)
                     /gene="eno"
                     /locus_tag="VW191_001700"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU9624437.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2568..3014
                     /locus_tag="VW191_001701"
     CDS             2568..3014
                     /locus_tag="VW191_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEU9624438.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3011..3814)
                     /locus_tag="VW191_001702"
     CDS             complement(3011..3814)
                     /locus_tag="VW191_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU9624439.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPSGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLCAFAQASIVSGID
                     TCLDLINFDKKVSDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAED
                     LPPLPFENIQAVFSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(3823..4468)
                     /gene="serB"
                     /locus_tag="VW191_001703"
                     /pseudo
     CDS             complement(3823..4468)
                     /gene="serB"
                     /locus_tag="VW191_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084485.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
     gene            complement(4527..5960)
                     /gene="glgA"
                     /locus_tag="VW191_001704"
     CDS             complement(4527..5960)
                     /gene="glgA"
                     /locus_tag="VW191_001704"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEU9624440.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSFGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDHILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(5957..7096)
                     /gene="glgD"
                     /locus_tag="VW191_001705"
     CDS             complement(5957..7096)
                     /gene="glgD"
                     /locus_tag="VW191_001705"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEU9624441.1"
                     /translation="MKIDKYSAILGNTVDFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7086..8228)
                     /locus_tag="VW191_001706"
     CDS             complement(7086..8228)
                     /locus_tag="VW191_001706"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEU9624442.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8218..10146)
                     /gene="glgB"
                     /locus_tag="VW191_001707"
     CDS             complement(8218..10146)
                     /gene="glgB"
                     /locus_tag="VW191_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEU9624443.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPEKKWKDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTVPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3101 a         2253 c         1811 g         3039 t
ORIGIN      
        1 aatccagacg cttatttgac tgaaatagct tctgaatttg gctgtcatcc aactaccatc
       61 cactatgcac tcaaagctat gggctacact cgaaaaaaag aaccacacct actatgaaca
      121 agacccagaa aaagtagcct tatttcttaa gaattttaat agtttaaagc acctagcaca
      181 ggtctaacaa atggtgaatt aatcgctcca atgacttacg aagagatgat gacgagcgac
      241 ttttttgaag tatggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt
      301 atagtgaaat gaaataagaa caggacaaat cgatcaggac agtaaaatcg aattctaaca
      361 atgttttaga agtagaggtg tactattcta gtttcaatct actatattaa tggacaatgc
      421 aagattccat agaatgggga agctagaact cttgtgtgaa gagtttgggc ataaactttt
      481 acctcttcct ccctactcgc ctgagtacaa tcttattgag aaaacatggg ctcatatcaa
      541 aaagcacctc aaaagggtat taccaagttg caataccttt tatgaggctc ttttgtcttg
      601 ttcttgtttc aattgactat ataaattgtc taagcgaaac aaccgataag aattggcaca
      661 aaagcgaccg tatttttgtt accaatacag gaaaaccagt tcatagttct atcttgagca
      721 agtctctcca gcgagcaaac gaacgcctta aaaaaccaat tcccaaacat ctgtcccctc
      781 acatcttcag acacaccact attagcatct tatcagaaaa taaaattcct ttaaaaacaa
      841 tcacggacag ggttggtcat tccgactctg aagtcactac ttccatctac actcacgtca
      901 caaagaacat gaaagatgaa gcaatcaatg tactggataa agttatgaaa aagatttttt
      961 aaaaagtttt gtcccttttt tgtcctctaa atacaaaaat agcccttcgg ataaaatccg
     1021 aggggctaga aacgttgtta aatcaacggc cgaacttttg aatttcatgg ttcgggataa
     1081 aatagttcac tgaactattt tattttttaa ggttgtagaa tgatttcaat ccacgatatt
     1141 cagctacttc accaagttgg tcttcgatac gaagcaattg gttgtattta gcgatacggt
     1201 ctgtacgtga aagtgaacca gtcttgattt gtcctgcgtt agttgcaact gcgatatcag
     1261 cgattgttga atcttcagtt tcacctgaac ggtgtgatac aacagcagtg taaccagctt
     1321 ctttagccat ttcgatagct tcaaaagttt cagtaagagt accgatttgg ttaactttga
     1381 taaggattga gttagcagca ccttcttgga taccacgtgc aaggtagtca gtgtttgtta
     1441 cgaagaagtc gtcaccaaca agttgtactt tcttaccaag acgttcagta agagctttcc
     1501 aaccatccca gtcgttttca tccataccat cttcaatagt gatgattggg tatttgttaa
     1561 ccaattcttc aaggtagtcg atttgttctg cagatgtacg aacagcagca ccttcacctt
     1621 caaatttagt gtagtcgtaa actttacgtt ctttatcgta gaattctgat gaagcacagt
     1681 caaatccgat aaatacgtct ttacctggta catatccagc agcttcaatc gcagcaagga
     1741 tagtttcaac accatcttca gttccttcga aacgaggagc gaatccacct tcgtcaccta
     1801 cggcagtttc caaaccacgt gatttaagga ttttcttaag agcgtggaag atttcagcac
     1861 cgtaacgaag ggcttcttta aatgttggcg caccaactgg caagatcatg aactcttgga
     1921 aagcgattgg agcgtcagag tgagaaccac cgttgatgat gttcatcatt ggagttggaa
     1981 gaactttagt gttgaatcca ccaaggtagc tgtaaagtgg gatttcaagg tagtcagcag
     2041 cagcacgagc tacagcgata gacacaccga ggattgcatt cgcacccaat ttacctttgt
     2101 taggagtacc gtcaagtgcg atcatagcac ggtcaatagc ttgttgatca cgtacatcgt
     2161 agccaatgat agcttcagca atgatgttgt ttacgttgtc aacagctttt tgtgtaccaa
     2221 gaccaccgta acgagatttg tcaccgtcgc gaagttcaac tgcttcgtgt tcaccagtag
     2281 aagctcctga tggaaccata ccacgtccga aagcacctga ttcagtgtaa acttctactt
     2341 caagtgttgg gttaccgcgt gagtctagga cttcgcgagc gtaaacatca gtaataattg
     2401 acatttttta ctctccttat gagttaaatt ttttacacct ctataatacc ttaaaacccc
     2461 tcctttttca agaaaaaacg ttatctttgt gcaacttttc cttaacttta taaagtaatc
     2521 gctttctttt gtctgtttta ttctaacttt tatgatatac tgttttcatg acagatttat
     2581 caaaacaatt acttgaaaaa gctcatggtg ggttaaaaat aaatccggat gagcaaagac
     2641 gctatcttgg tacttttgag gaaagagttc ttggatatgt agatattgac acagcaaata
     2701 gccctcagtt agaaaaaggc tttttattta ttttagaaaa ccttcaggaa aaagcagagc
     2761 cactatttgt gaagatttca ccaactatcg aatttgataa gcaagttttc tacttaaaag
     2821 aagcaaaaga aactgatagt caagccacca tagtatctga agagcatatt acttctcctt
     2881 ttggcctggt tattcatagc aatgcaccag ttcaagtaga agaaaaagac cttcgacttg
     2941 cttttccaaa actttgggaa gttaaaaagg aagaaccagc caaaacatcc ttatggaaga
     3001 aatggtttag ctaaatcttg cacatattta ataagtgccc aatattggca gccgtgcgct
     3061 ccagatagag actggcattt ttcaaactat cttctaaagg ttcacttttc tccaaaatag
     3121 aaaagacagc ttggatattt tcaaatggta ggggaggtaa atcttcagca agactaccgc
     3181 aaatagcaat aacaggaact ccaacagggg ttctttttgc aacacctata ggcgctttcc
     3241 cagcaaagct ttgactatca agtcttcctt ctccaacaac aaccaagtca gcatctgaaa
     3301 ctttcttatc aaagttgatt aagtccaagc aggtatcaat tccagacacg atacttgcct
     3361 gagcaaaggc acacaaacca gcagcaatcc ccagtcctcc atcattactg gccgtgccac
     3421 caacaccgat ataaatatct ttaatccctt tagaaatgag atggagaatc aactctccaa
     3481 taccacaagt ttggatttga agtggatttc gtttctctag cggaattttt ccaagaccaa
     3541 ccaagtcagc tacttcaaat agtgccagtt cccctttttg aaaatagcgc atggcttctt
     3601 tttgtccaga agggtctgtc acttggatcc atttttcttt taggtcaaga gaatgtcgga
     3661 tagcatctac agtaccttct cccccatcac caacagggca gaggagacat tctacatctg
     3721 ctatcgattg ttggaagcct ctttttattg cttcagctac ctgttgagct gtcaagcttt
     3781 ccttaaacga atccggtgca attacaatct tcatattttc cctcattcta aacagtcaat
     3841 caaaggaaga acttctaaaa aatccctctt gtcaacatga tgtggtattt cttttttgag
     3901 cacttctttg gcacaaaagg cgattcctaa ctccgccgac ttcaacatta atagattatt
     3961 aaccccatca ccgattgcca cccttctttc tttagaaagt tttagtttct ttctccattt
     4021 ttccagagtc tcttttttga cctggggact tataatttgt ccaactaatt ttcctgttaa
     4081 aagaccttct ttgacttcaa gctggttggc agtgaaatag gcaataccaa gggattttgc
     4141 taatctctcc aactattggt gtaaatccac cagacaccag accaactagg atgccattct
     4201 tttggagaat agagatgaac tctgggacat ttagcgatag atgaattgag ttgaagacgt
     4261 tatcaaagac caaaatagga agaccttcca acaaggacac tctttttctt aaactgcttt
     4321 caaagaccaa ctctcctcgc attgcccgac ttgtaatctg cgaaatttcc gcctcatgac
     4381 ctgcctctct ccctaaaaga tcaatcactt attctaggat taaggttcca tctacatcca
     4441 aaacacacaa gccttttact tgagacatca gttctcctct ctaaacagcc taaaaatcgt
     4501 atgaagtcat catacgattt tatctattaa ttaactaaac tatggtacaa gtcaaggtat
     4561 gacttgcagg ctgtatccca tgagaagtca ctctccatag cttgtttttg taggtttctc
     4621 caaatgtctg gatggtttct atacaagtcc aatgctgttt ggaaagtcca atttaaccaa
     4681 taaggagata gattgtcaaa gctaaagcca gtaccgcttc cttcgattgg attgaaagcg
     4741 cgaactgtat ctcgcaagcc tccaacttca tggaccaatg gcaaggttcc ataacgcata
     4801 gccatcattt gagacaagcc acacggttca aaacgacttg gcatgaggaa gaggtcacaa
     4861 gcagcgtaga tttcctgagc aagtttgaca tcaaaagtga tatttgttga tagcttgtct
     4921 gggtaaatct gagcaaacca tgagaaagct ccttcaaagg ctggatcgcc agttcccaaa
     4981 agaacaatct gaacatcttc ttgcaagata tggtgaagac tttcgaccac cacatcaaaa
     5041 cctttttgac gtgtcaaacg agaaacaatt cccaccagtg gaacgtctgc tctaacaggc
     5101 aagccaactc tttcttgcaa ttttgcctta tttttggctt tcccagacaa atcttcctga
     5161 ttgaaatgat agtctaaaag agcatccgtc tgaggattat aaagatcagc atcaatccca
     5221 ttcacgatac cagatacttt accagactcc attttaagaa tatgatccaa attacatcca
     5281 aactgactag tcataatttc atgagcatag ctaggtgaaa cggttgaaac acggttcgca
     5341 tagagaatac ctgccttcat ccagttcaga cagttgttcc atcgaagggt gccatcagcg
     5401 taacgttcaa agccaactcc aaacaaatca cccaacattc cttctgaaaa ttgtccttgg
     5461 aattctaaat tatgaatggt taaaactgtt tcaatgtcct cataggcttg aatccaacgg
     5521 tatttttcct tcaacaagaa aggaatcata gctgtatggt agtcatgaac atggagaaga
     5581 tcaggaataa agtcaatcct ttccatagcc tcaatggcag ccagttggaa aaaggcaaag
     5641 cgttctccgt catcaaaatc accgtaaaca tgaccacgga agaaataata ttgattgtca
     5701 ataaagtaga aggttacacc atttaatact gttttcttaa ttccacaata ctgtctgcgc
     5761 caaccaaagc tcacctcaaa atgaagcaca tcttcaatct gatttccaaa tttagcctct
     5821 accatatcat agtagggtaa aatcactgca acttcgtgcc cagcttttac cagtgatttt
     5881 ggaagagcgc caatgacgtc tcccaaacca cctgtttttg aaaagggtgc accctctgct
     5941 gctacaaata aaattttcat gaatgaatat cctctgttac tttagcacct ttcttaacca
     6001 caactggatg ttctgcagtt cctcgaatca caacaccagg ctcaacttca acccctttgt
     6061 ccaagatagc atattcgacc tgagcccctt ctccaataac aacacgaggg aagagcaggc
     6121 tatctttaac caagctatcc ttatggacat gaatattacg tgatagaaca gaattagcta
     6181 cttgaccttc aataatacta ccagaggcaa actgagaagt gcttacctta gatgtattag
     6241 catagtaagt tggctcttcg tttttgacct ttgtataaat cttttggttt ggtgagaaaa
     6301 gagaatagaa tttttgtgat tcaagcatat cgatattcgc ttgataataa gatttaacag
     6361 agtgaatatt ggctagatag cccgtgtact cgtaggcaaa agctccctct tttacagcca
     6421 aatcccgtaa aacatagcgc aatttctctg gatgttcttt tttagcttct tcttccaagt
     6481 gttcaatcaa ccaaggtgta tcaacgacaa agatatctgt agacatattg aacgtttcag
     6541 ctgttgactt gctatcaaag agtttatgag aaagaacatg gtctgtttca tctacatcca
     6601 agattgcatt tacttctgaa atatctttct tagctagttt tttataaact acagtgatag
     6661 gctcttttgt tgtactatgt aggtggaaaa cttggttcaa atcaatgtta ataagaacat
     6721 cgcagttgag ggcaaccgtt tggtttgagc cagaacgttt caaataagta agaagctgtt
     6781 ggtagtattc ttttccaact gtactacttt ctacacgggt attgtaaatt cctagatagt
     6841 aatggctaag aagggttgat aagccccact cgcgtcctga acgaatatgg tcaaatactg
     6901 agctgatatt atcctgctgg aaaataccaa agacactacg aacacctgca ttagcaaggc
     6961 ttgaaagtgg gaagtcaatc aaacgatatt tcccaccaaa tggcaaactt gctactggac
     7021 ggtggtccgt caatgtcgac atattgtgaa aatcaactgt atttcctaaa atggcagaat
     7081 atttatcaat cttcatctgt tgctaccccc actacttcat tatatcctac aacttgtact
     7141 tcatctgttc catcaatttc gacaccgtca gaaataatcg caccttcacc aataatggca
     7201 cgtttaatct tagctccttg accaatgata gctccactca tgataactga atcaaggact
     7261 tccgctcctt cgcgaacttg cgcgcctgtt gaaaggatag aatgtttaac agttccatca
     7321 acgaaacatc cgtctacaac taatgagtct tccacatgag catttgcccc gaggaagttt
     7381 ggtggtgaaa tcaagtttct tgagtaaatc ttccattgac ggttacgact atccaaggca
     7441 ttttctggag aaatatactc catgttcgct tcccaaagtg actcaatagt accaacatct
     7501 ttccaataac cactaaattc gtaagcataa acactttcac ctgactcaag gtaatttgga
     7561 atgacatttt taccaaagtc tgacatgcca accttgctct tttcagcagc gactaacata
     7621 ttacgaaggc gttgccaatc aaaaatgtag attcccatag aagcttttgt agatttaggt
     7681 tgagctggtt tttcttcaaa ttcaacaata cgattgttag catctgtgtt catgatacca
     7741 aaacggcttg cttctttaag agggacgtct aaaactgcta ctgtcaagct ggcattatta
     7801 tccttatgag actggagcat atcatcatag tccattttgt agatgtggtc cccagacaaa
     7861 atcaagacat actcaggatt gacactgtcg atatagtcga tattttggta aatagcgtga
     7921 ctagtcccct caaaccaacg atttccttca cttgcagaat aaggttgaag aatagagaca
     7981 cctgaattaa taccgtctag tccccagctt gaaccattcc caatatggtt gttgagagca
     8041 agtggttgat actgtgtaac aaccccaaca ttatgaatcc ctgagttggc acagtttgat
     8101 agggcaaagt caatgatacg gtagcgccca ccaaattgca cagctggttt tgcgatgctt
     8161 tgagtgagtt taccgagacg agttccttgc ccaccagcaa gaatcaaagc taacatttca
     8221 tttttcattt tctactcctt tttggttttt atttgtgacg gttttagtag atttcaagcg
     8281 acgtttgatt ttccatacac ttgctcccat agccggtacg gtaaaggtta aggtctgctc
     8341 ataatctttc catagtcctt cttgcgtttg aacagtttga ttatgttctt tccaaacgcc
     8401 tccccactct tccaactcag tattccatac ttcttcgtaa attcctgcaa cgggtagtcc
     8461 gattgtaaaa tctttccgct caacaggtac catgttaaag atacagacta acatttctcc
     8521 ctttttaccc ttacgaataa aggaaagaac actctggtct cgattatccg catcaatgat
     8581 ttcaatacca tcatagctgg tatcgatttc ccacagacag cgatgatctt tgtaaaactg
     8641 gtttagctga gaagtgaaat acttcatctt agcattcatt gggtcttcta ggttagacca
     8701 ttccaactgt tcttcagatt tccattctag gaattgaccg tattcgctac ccatgaagag
     8761 caatttctta ccagggtgac aaatttggta cgtatagaga ttgcgcaagc ctgcgaattg
     8821 attgtaacga tctccccaca tcttatgcat catactcttc ttgccatgaa ccacttcatc
     8881 gtgcgagaat ggcaagagat aattctcctt gaaaacatac ataaagctga aagtcaccag
     8941 gttaaagtca tatttacgat aaatcggatc ttcttcgtag aaacggagga tatcattcat
     9001 ccagcccatg ttccatttgt agtcaaatcc tagaccacca atctctttca ttcccgtaat
     9061 cttggttgca gacgaacttt cttctgcaat catcatcaca tctggatatt ctaacttaat
     9121 aacttcattc aagcgttgaa ggaaataata accttcatag ttgagatttc cgccatcttt
     9181 attaggtgtc catggagcat catcatagtc caaatagagc atgttgctaa cagcatccac
     9241 acgaatacca tccaaatgat agacatcaat ccaatgctta atgcaagaaa ttaagaagga
     9301 ctggacttca ttttttccaa ggtcaaaatt aagggcaccc caaccatggt tatgagcctt
     9361 attatggtct tggtattcaa aagtcggtgt cccatcataa taggctaagg catcatcatt
     9421 gatggtaaag tgacctggta cccagtccac aataacccca atattatggg tatgacactc
     9481 ctcgacaaaa tcttgaaact cctctggtct accataggca tgctctaaag cgaagtaacc
     9541 cataagctgg tacccccaac tcaaacctag tgggtgggac atcaagggca taaactcaat
     9601 atgagtatag ttcatctcaa ctaggtaagg aatgagttca tctttcaatt gggcaaaact
     9661 ataaggactg ccatcaggat ttctcttcca agagccagca tgaacctcgt aaatattgac
     9721 aggacgttca gcaaagcccc aacgttttct tcgtgccagc caaagtccat ccttccattt
     9781 cttctcagga agctctgtta cgattgcccc tgttcctgga cgagcctcat acctgacagc
     9841 aaaagggtca atcttcatca gttgatgacc attttgacgt gtgacatgat atttgtaaat
     9901 atgcccttct tgagccatat tggtaaagac ttcccagacc ccaaaatcat ttcttaccat
     9961 tggaatctga ttttcaatcc agttggtaaa atcaccaacc aagtgaacag cctgagcatt
    10021 aggtgcccaa acacggaagg tatagccatg ctctccattt agttcttccc tatgtgctcc
    10081 tagataatgt tggagataaa aattttcacc cgtcataaag gtttttaatg cttctctatt
    10141 atccatatac tccccttctc ctataagcgt tttctatgtt tttattaaac taccttttta
    10201 gaga
//
LOCUS       DAVKJU010000066        10161 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVKJU010000066 DAVKJU010000000
VERSION     DAVKJU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.066"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            4..77
                     /locus_tag="VW191_001708"
     tRNA            4..77
                     /locus_tag="VW191_001708"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Asn,seq:gtt)
     gene            complement(171..968)
                     /locus_tag="VW191_001709"
                     /pseudo
     CDS             complement(171..968)
                     /locus_tag="VW191_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1233..1469
                     /locus_tag="VW191_001710"
     CDS             1233..1469
                     /locus_tag="VW191_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEU9624444.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            1700..2986
                     /locus_tag="VW191_001711"
     CDS             1700..2986
                     /locus_tag="VW191_001711"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU9624445.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            3186..3653
                     /locus_tag="VW191_001712"
     CDS             3186..3653
                     /locus_tag="VW191_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU9624446.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            3686..3779
                     /gene="ffs"
                     /locus_tag="VW191_001713"
     ncRNA           3686..3779
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VW191_001713"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3839..4282
                     /locus_tag="VW191_001714"
     CDS             3839..4282
                     /locus_tag="VW191_001714"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU9624447.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            4284..4799
                     /locus_tag="VW191_001715"
     CDS             4284..4799
                     /locus_tag="VW191_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9624448.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            4813..6174
                     /gene="radA"
                     /locus_tag="VW191_001716"
     CDS             4813..6174
                     /gene="radA"
                     /locus_tag="VW191_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU9624449.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            6247..6744
                     /locus_tag="VW191_001717"
     CDS             6247..6744
                     /locus_tag="VW191_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU9624450.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            6769..7552
                     /locus_tag="VW191_001718"
                     /pseudo
     CDS             6769..7552
                     /locus_tag="VW191_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            7697..8665
                     /locus_tag="VW191_001719"
     CDS             7697..8665
                     /locus_tag="VW191_001719"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEU9624451.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(8799..9080)
                     /locus_tag="VW191_001720"
                     /pseudo
     CDS             complement(8799..9080)
                     /locus_tag="VW191_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9207..9440)
                     /locus_tag="VW191_001721"
     CDS             complement(9207..9440)
                     /locus_tag="VW191_001721"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624452.1"
                     /translation="MFNQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(9433..9549)
                     /locus_tag="VW191_001722"
     CDS             complement(9433..9549)
                     /locus_tag="VW191_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEU9624453.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(9764..10117)
                     /locus_tag="VW191_001723"
     CDS             complement(9764..10117)
                     /locus_tag="VW191_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEU9624454.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
BASE COUNT         2822 a         1682 c         2398 g         3259 t
ORIGIN      
        1 taatccgcca tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc
       61 gagtcctact ggcggagtaa tttagttaag aggtttagac ctctttttta ttatgtaata
      121 ataatctatt tcgtgttata gtaaaatgaa ccaaaaatag tggctctttg tcaactgtag
      181 tgggttgaag aaagcgaaga tctagaaagg acaaatttcg tcctttcttt tttgaagttt
      241 tcaaagttcc taaaaccaaa ggcattgtac ttgataagtt tgatgagatt attggtggct
      301 tccagtttgg cgttggaata aggtaattga agggcgttga cgattttctc tttatctttg
      361 aggaaggttt taaacagagt ctgaaaaaga ggtggaaaag caagagctga tagagattat
      421 agtggtgttt caagtcttcg gaatagctca aaagtttatc tagaatttct ttattagtca
      481 agtgcatacg aaaagtaggg cgataaaatc gtttatcact cagtttctga ctatcctgtt
      541 gaatgagctt tcagtatcgc ttgatagcct tgtattcatg ggatttcgga tgatggcttg
      601 tgttctgctc tcaagaacag ttatgatatt gagcttatca aagtcttgag caataaagct
      661 catctccatc tcccgattga aacagtcact ccccggactg tttcaacgtc ctaggacata
      721 atctcaggaa gacgcgaaaa atcatgctca aagtgaaaat cattgatctt gcgaatgaca
      781 gttgaagttg aaatagacaa ctgatgatca atgtcggtca tagaagtctt tttaattagc
      841 ttctgagcaa tcttttggtt gatgatacaa ggaatttgat gattcttctt gacgatagaa
      901 gtttcagcga ccctcatttt tgaacagtga tagcacttaa aacggccttt tctaagaaga
      961 attctagttt gaatttttta tactagaaaa tcagaaccat aatacctata taaaaatatt
     1021 atagttctaa taggatttac ccaaaagttt taaggcggtc tttttagaac ttaaatcgtt
     1081 tgaaatttag gtagcaaatt tgtttctatt ctgtcaactt ttcctatttt tatcttgttg
     1141 agcctggtat tttaacaatt caggaattga tagtgaatgt gtaaaatttt ttgttagaat
     1201 aagtttataa aaaagaaaag gagtatttga ttatgttaca aaaaatttat gagcagatgg
     1261 ctaatttcta tgatagtatt gaagaagagt atggtcctac atttggtgat aattttgact
     1321 gggaacatgt tcattttaaa tttttaattt attatttagt gagatatggc attggttgtc
     1381 gtagggattt tatcgtttac cattatcgtg ttgcttatcg tttgtatctt gaaaaattgg
     1441 taatgaatcg gggttttatt tcttgttgag gtaattttag taaatttccg aactaattta
     1501 ctcttttatg gaaagataat agtaaatagc tagtaatttt tctaaatcat tttttaatag
     1561 ttggaaatag caaatctttc tattgtttct tcttgataaa aaggcgattt tttattataa
     1621 taaattgtaa gatataattg caggtgagag tcctgccatg tatgtgagaa aggaagagcc
     1681 tgagggctca gacaagatta tgacttcagt tgttgttgta ggtacccaat ggggtgatga
     1741 aggtaaaggg aagattacag acttcctttc agcgaatgca gaagtgattg cacgttacca
     1801 aggtggtgat aatgctggtc acacgattgt gattgacggt aagaaattta agttgcactt
     1861 gattccatct gggattttct tccctgaaaa aatatctgtc attgggaatg gtatggttgt
     1921 aaatcctaaa tctcttgtaa aagagttgag ctatcttcat gaggaaggtg taacaactga
     1981 taacttgcgt atttctgatc gtgcgcatgt tattttgcct tatcatatcg agttggatcg
     2041 cttgcaagaa gaagctaagg gcgacaataa gattggtacg acaattaagg gaattggtcc
     2101 agcttatatg gacaaggctg ctcgtgttgg aattcgtatt gcagatcttt tagataaaga
     2161 tattttccgt gagcgtttag aacgtaacct tgctgaaaag aatcgtcttt ttgaaaaatt
     2221 gtatgacagt aaagcgattg ttttcgatga tatttttgaa gaatattacg aatatggtca
     2281 acaaatcaag aaatacgtga tagatacatc tgttatcttg aatgatgcgc ttgataatgg
     2341 caaacgtgtg ctttttgaag gtgcacaagg tgttatgcta gatattgacc aaggtactta
     2401 tccatttgtt acgtcatcaa accctgtagc tggtggtgtg acaattggtt ctggtgtcgg
     2461 tccaagcaag attgacaagg ttgtaggtgt atgtaaagct tatacgagtc gtgtaggaga
     2521 tggtcctttc ccaactgagt tgtttgatga agtgggagaa cgtatccgtg aagtgggtca
     2581 tgagtatggt acaacaactg gtcgtccacg tcgtgtaggt tggtttgact cagttgtgat
     2641 gcgtcatagc cgtcgtgttt ctggtattac taacctttct ttgaactcta ttgatgtttt
     2701 gagcggtttg gatactgtga aaatctgtgt ggcctatgat cttgacggtc aacgtattga
     2761 ctactatcca gctagtcttg agcaattgaa acgttgcaag cctatctatg aagagttgcc
     2821 aggttggtca gaagatatta ccggagttcg caatttggaa gatcttcctg agaatgcgcg
     2881 taactatgtt cgtcgtgtga gtgaattggt tggcgttcgt atttctactt tctcagtagg
     2941 tcctggtcgt gaacaaacaa atattttaga aagtgtttgg tcctaagaga tttttaagat
     3001 ttgtttaaga taggtcgggt atactataga cggttacaag aagacctcct aacttgttgt
     3061 aacaaatatc ctaaactttt ctttttcata ataatctccc tatagagtca ccgcattcgg
     3121 tggctttttt tgtgttggga ttcatgatat aataataaaa tcgataagta ggaaaaggaa
     3181 aagagatgta ttatacgctt gaagaaaaag aagtctttat gagggaggct ttgagagagg
     3241 ctgagattgc tcttgaacac gatgaaattc caattggttg tgtgattgtc aaagatgggg
     3301 aaatcattgg tcgtgggcat aatgcgcgtg aggaattaca gcgagcggtt atgcatgcgg
     3361 aaattatggc tatagaggat gcgaacttga gtgaggagag ctggcgcttg ctggattgca
     3421 cactttttgt gaccattgaa ccttgtgtca tgtgtagtgg ggcgattggg cttgcccgca
     3481 ttccaaatgt ggtctatggg gctaaaaacc agaaatttgg cgctgctggg agtttgtacg
     3541 atatcttgac agatgagcgt ctcaatcatc gtgtggaggt tgaaatagga attttggaag
     3601 atgaatgcgc agctattatg caggactttt ttagaaatag acggaaaaaa taattttgct
     3661 tttaaaatga ataggaatgt gatataataa atagtggagc aacagttctg cgtgaagcgg
     3721 gtcaggggag gaatccagca gccctaagcg atttgaattg tgtgctcttt tttcgtgctt
     3781 tttccgaata aataagatag aataatctag aataaatgat aatagaaaag agaaaattat
     3841 gaaaattcgt ggttttgaat tggtttcgag ttttacagat gaaaatttat tgcccaagcg
     3901 tgagacagcg catgcggctg gttacgactt aaaggttgct gtgcgtacag ttgttgcgcc
     3961 aggagagatt gtcttggttc cgacaggggt taaggcttat atgcagccga ctgaggttct
     4021 ctacctctat gatcgttctt caaatcctcg taagaagggc ttggttttaa ttaactcagt
     4081 tggggtcatt gatggagatt attatggaaa tcctggaaat gaagggcata tttttgcgca
     4141 gatgaagaat atcacagacc aagaggttgt tcttgaagtt ggggagcgta ttgtccaggc
     4201 tgtttttgct actttcttaa ttgcagatgg agatgcggct gatggcgttc gaactggtgg
     4261 atttggatcg acagggcact agaatgaaga ttatctttgt acgtcatggg gagccagatt
     4321 accgtgagtt agaggagcgt tcttatatag gatttgggat agatttggca cccttgtctg
     4381 agatgggacg gcagcaagtc cagaaattga gcaaaaatcc tttactctcg tcagctgaaa
     4441 taatcgtatc ttctgcagtc acaagagctt tagaaacggc ttcgtatgtg gtctgtgcta
     4501 cgggtcttcc tttaagagta gagcctttat tacatgaatg gcaggtctat aaaacaggaa
     4561 tagaaaactt tgaaacagct agaagactgt ttttagaaaa caagggggag ttgcttccta
     4621 atagtcctat tcaatatgag acagctacgg aaatgaagtc tcggtttcta gaatgtatgt
     4681 ctaagtatcg agaacatcag actgtggtag ttgttgctca tcgaatgctc atgcgccagt
     4741 ttgtgccaaa tgagaagatt gatttttgcc aagtgattga gtgtgagtta gagatataga
     4801 aagaggtttg tcatcgcaaa gaaaaaagcg acatttgtat gtcaaaattg tgggtataat
     4861 tcccctaaat atctgggacg ttgccccaac tgtgggtctt ggtcttcttt tgtggaagag
     4921 gttgaggttg ccgaggtcaa gaatgcgcgt gtgtccttga caggtgagaa aaccaagccc
     4981 atgaaactag ctgaggtgac ttccatcaat gtcaatcgaa ccaagacgga gatggaggaa
     5041 ttcaaccgtg tgcttggagg cggagtggta ccaggaagtc tcgtcctcat cggtggggat
     5101 cctgggattg ggaaatcaac ccttctccta caagtctcaa cccagttgtc ccaagtgggg
     5161 acagttctct atgtcagtgg ggaggagtct gcccagcaga ttaaactacg tgcagagcgc
     5221 ttaggtgata ttgatagtga gttttatctc tatgcagaga ccaatatgca gagtgttcgt
     5281 gcagaagtgg agcgtatcca gccagacttt ctcattattg attccatcca gaccatcatg
     5341 tctcctgaga tttcaggggt gcaggggtct gtttctcagg tgcgtgaagt gaccgctgaa
     5401 ctcatgcagt tggccaagac caataacatt gccatcttta tcgtaggtca tgtgaccaaa
     5461 gaaggaacct tggctgggcc tcgtatgttg gagcatatgg tggatacggt gctttacttt
     5521 gaaggggagc gtcaccacac ctttcgtatt ttgagagcgg tcaaaaatcg ttttggttcc
     5581 actaatgaga ttgggatttt tgagatgcag tcgggcggct tggttgaggt actcaatccg
     5641 agtcaagttt tcctagaaga gcgtttggat ggggcgactg gttcctccat cgttgtaacc
     5701 atggaaggga cgcgtccgat tttggcggag gttcaggctt tggtaacacc gaccatgttt
     5761 ggaaatgcca agcgtactac gacaggactt gattttaacc gtgctagctt gattatggct
     5821 gttttggaaa aacgggcagg gcttctcttg caaaatcagg atgcctatct caaatctgct
     5881 ggtggtgtta aattggatga acctgcgatt gacttggctg ttgcagttgc tattgcttcg
     5941 agctacaaag acaagccaac taatcctcag gaatgttttg tcggagaact gggcttgaca
     6001 ggagagattc ggcgcgtgaa tcgtattgag caacgcatca acgaagctgc taaactgggc
     6061 tttactaaga tttatgtacc taagaattcc ttgacaggaa tcactctgcc taaggaaatt
     6121 caggtcattg gcgtgacaac gattcaggaa gttttgaaaa aggtctttgc ataatccgtg
     6181 acaaattctc ttaaaaatga taagatagga gaaatatttg actatcaaat tttcaaggag
     6241 ggaatcgtgt cgtattttga acagtttatg caagccaatc aggcttatgt tgccctacat
     6301 gggcagttaa atctgccact taaacccaaa acaagagtag ctattgtgac ctgtatggac
     6361 tctcgtctgc acgttgcgca agctctgggc ttggcacttg gggatgctca tatcttgcgg
     6421 aatgcaggtg gtcgagtgac tgaagacatg attcgttcgc tagttatttc ccagcaacaa
     6481 atggggacaa gagagattgt ggtattgcac catacagact gtggtgctca gacctttgaa
     6541 aatgaacctt ttcaggagta tttaaaagag gaattaggtg tagatgtgtc agaccaggac
     6601 ttcttgccct tccaagatat agaagagagt gtacgcgagg atatgcaact gcttatcgag
     6661 tctcccctaa taccagacga tgtcattatc tctggtgcca tttacaatgt tgatacagga
     6721 agtatgacag tcgtagaatt ataaatactt catttagaaa gaaagtgtat gaagaaaagc
     6781 agtattttat ttatttttat tttattgcta tgtattggtt tacagtatga aaccatctac
     6841 tatacggacg gttcgaggtc aggtgcggaa tatggactaa tgggagtttc tatctttcta
     6901 gctctctttt acatgattcc ggctctttat tttctcttcc gtattgggaa aaaatgggaa
     6961 ttgccaaaga aggttttgat tctgtcttta ttgggaggga tgttcctttc aggctggttg
     7021 tctagttttg ctaatactta tatccatgat ttactggggg ttcttttccc agatagtaca
     7081 tttttaaatg cctttgaaag tgctattgtg gctcctttgg tagaagaacc cttgaaatta
     7141 ttgccacttg tttttgtttt ggctttgatt cctgtgcgaa aattaaaatt tttgttttta
     7201 cttggaattg cctccggttt gggattccaa atgattgagg atattggtta tattcgtacg
     7261 gatttgccag agggctttga ctttactatt tcgcgaattt tagagcgtat catctcagga
     7321 attgcctctc actggacttt ttcaggtcta gctgtagtag gtgtttactt gctttacaga
     7381 gcctataaag gacagaaggt tggcaagaaa cagggtctta ttgtttaact ctccttttgt
     7441 ggagttggaa acagagttgc ctttagcgat tccagtggtt acggctattg ctctctatgg
     7501 ttattatcat gcttattgct ttgttgagaa acacaatgag ttgatgacct agaatatttt
     7561 tcaaaagaat gatgcaaggg tacaaatatg gtgcccttct tttatttttg attgaaaaat
     7621 agtgcaaaaa gcgctacaat ggtagatgga aaatcttgtg aaaagcacaa gcgatacata
     7681 tataccggag gaaatcatgt ctttttctga tttaaagctg tttgcccttt cttctaataa
     7741 agaattggca gaacgtgtgg cgcaggagat tgggatagag ttggggaaat caagtgttcg
     7801 ccaattttca gatggagaga ttcaggtcaa cattgaagaa tcaatccgtg ggaaacacgt
     7861 ctttatctta caatcaacta gttcgcctgt aaatgacaat ctgcttgaaa ttttgattat
     7921 ggtagatgct ttgaagcgtg cgagtgcaga gtctgtcaat gttgtcatgc cttactatgg
     7981 gtatgcacgt caggatagaa aggcgagagc gcgtgagcca atcacttcaa aacttgtcgc
     8041 aaatatgctt gaagtagctg gagtggatcg tttattgacc atcgacttgc atgctgcgca
     8101 aattcaagga ttctttgata ttcctgtgga tcatttgatg ggtgctcctc taattgcaga
     8161 ttattttgag cggcgtggta tggttggttc tgactatgtg gttgtcagcc cggaccatgg
     8221 aggggtgact cgtgctcgta agttggcaga atttttgaaa acatctatcg ctattattga
     8281 taaacgtcgt agcgttgata agatgaatac tagtgaagtt atgaacatca tcggtaaggt
     8341 tgaaggcaag acttgtatct tgattgatga tatgattgat accgctggaa cgatttgtca
     8401 tgcggcagat gctcttgcgg aagctggtgc tgttgaagtc tatgcaagct gtacgcaccc
     8461 agttctttct ggtcctgcta cggacaatat ccaaaaatca gctattaaga aattggttgt
     8521 tttggatacc atctatctgc cagaagagcg tttgattgat aagattgagc agatttcaat
     8581 cgctcatctc ctaggggatg ctatcgtacg tattcatgaa aaacgaccac tttctccact
     8641 tttcgatatt gagaaaaaaa tttaatgacc aagcctgaga tgattctcag gttttttcgt
     8701 cttttttccg aataaatgga tagtagcggt gaatctagta aagctagatt taaaactgtg
     8761 atataataaa aagagaagaa atatgactat acgtcatcag aagtttcagc gaccatcatt
     8821 tttgaacagt gatagcactt gaatcgacgc tttctaagga gaattctagt aggtatacca
     8881 gtcgtttcaa gataaggaat tttagaaggt ttttgaaagt catatttctt caattggttt
     8941 ccgcactcag ggcaagatgg ggcgtcgtag tccagtttgg cgatgatttc cttgtgtgta
     9001 tccttattga tgatgtttaa aatctggaca ttagggtctt taatgtctag taattttgtg
     9061 ataaaatata attgttccat atgattcttt ctaatgagtt gttttgtcgc ttttcattat
     9121 aggtcatatg ggactttttt tctacacaaa ataggctcca taatatccat aggggattta
     9181 cccactatgg atattatgga gccattttac aacagtgcct caaattcaga tactgacata
     9241 cctaattgct ggctggcaac ctcagaagtc agaagacctt ggcgtactat gtctaggaag
     9301 gcaaaaagtt tcctttctgc ccttccttca actttcccac gctctagtcc ttgttcaagg
     9361 ccttcagccc tagcagtttc caaggcatag tcctgtgcta acaagacttg ttcttctcgc
     9421 atatgtagtt gattaaacat tttcctgtcc tcctcggacc agctcttgta gtccagcagt
     9481 tgatttgctt gggtaatggc tcgttgcggt tgctgggtaa agggcttgtt gccgaaaaac
     9541 tccaaccatg gcttgcgaac cttgtctttg ctggtttctc tgtatttttt aattccaaga
     9601 atgccatctt aaccagatgg ttttcctgtc cattgttggt aatcgccaat acctcacctg
     9661 ttgtgtcttc gcgcatacta aagctatgaa aagccaggtc atctgagaaa taattactat
     9721 ccacgatagc aatggcgtaa acaggagcga tgtgtttgta gctctagtga gtatcacctt
     9781 ctcgctgacg aattttttca agattttgat taacctgact gcacaggtaa gcccacaagt
     9841 gattgatgaa aaaattctga tgatggactt gaatctcaat aattacttga gtaccgttat
     9901 ccaactccgc caagacgtct atactggtat aaaaatcctg cgctgagtag ggcatggaag
     9961 gcaagacatg aatgttactt ccctccaaaa tagtcacatt tttggctggc aagtccagca
    10021 tatcgcggat aaattgacaa gtgatttctg ggttgctgaa aattttctta gcaaccaagt
    10081 cattggtcgg gctaattccc agatgtctga gaatcatcct tttcctcctt ctatagtgaa
    10141 ttgaaataag atgtgaacaa c
//
LOCUS       DAVKJU010000067         9806 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVKJU010000067 DAVKJU010000000
VERSION     DAVKJU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9806
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.067"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            261..353
                     /locus_tag="VW191_001724"
                     /pseudo
     CDS             261..353
                     /locus_tag="VW191_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(609..1229)
                     /locus_tag="VW191_001725"
     CDS             complement(609..1229)
                     /locus_tag="VW191_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9624455.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1229..1711)
                     /locus_tag="VW191_001726"
     CDS             complement(1229..1711)
                     /locus_tag="VW191_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEU9624456.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALGLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1711..2553)
                     /locus_tag="VW191_001727"
     CDS             complement(1711..2553)
                     /locus_tag="VW191_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEU9624457.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2648..3448)
                     /locus_tag="VW191_001728"
     CDS             complement(2648..3448)
                     /locus_tag="VW191_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEU9624458.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3592..4971
                     /gene="glmU"
                     /locus_tag="VW191_001729"
     CDS             3592..4971
                     /gene="glmU"
                     /locus_tag="VW191_001729"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU9624459.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4981..5526
                     /locus_tag="VW191_001730"
     CDS             4981..5526
                     /locus_tag="VW191_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9624460.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            5538..5852
                     /locus_tag="VW191_001731"
     CDS             5538..5852
                     /locus_tag="VW191_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEU9624461.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5869..6561
                     /locus_tag="VW191_001732"
     CDS             5869..6561
                     /locus_tag="VW191_001732"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEU9624462.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6638..7129
                     /gene="rocS"
                     /locus_tag="VW191_001733"
     CDS             6638..7129
                     /gene="rocS"
                     /locus_tag="VW191_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEU9624463.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7227..7817
                     /locus_tag="VW191_001734"
     CDS             7227..7817
                     /locus_tag="VW191_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU9624464.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7826..9589
                     /locus_tag="VW191_001735"
     CDS             7826..9589
                     /locus_tag="VW191_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU9624465.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAMLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3026 a         1701 c         2087 g         2992 t
ORIGIN      
        1 ttagattttt tctgtctaac ttttgggggg cagttcattt aacacctgat aatatgcgtt
       61 tttttatttt aaagactttt tgaccagcct cattttttta acttgatact cagtgaaaag
      121 ccaaaattaa actaggaagc tagctgtagg ctgctcaaag aacagctttg aggttgcaga
      181 taaaacttgt gaggtcacca acatatataa tgtgaagctg acatggtttg aatagatttt
      241 agaagggtat gagtctggaa cttttaatgg ataatgcaag attccataga atgggtaagc
      301 tagagttctt atgtgaagag tttgggcata aacttttacc ttttcctccc tacttatctt
      361 agtatagaaa agtgaatctg aaatagtaca taactgcttc taaaacattc ttataatatg
      421 atttaaattc tcaaatcata ttattcagtt cttatttcat tttgctctac aatcctgttg
      481 agaagacacg tgttcatatc aaaaaggtat tggcaagttg caataccttt ttacaaggtt
      541 cttttgtctt atttttgttt caactgacta tatctcctat ggttctagtt cagaaggcta
      601 ggctataatt atgattgata agaagtatca ttccaagtat tgagagtgaa tgtttcaaaa
      661 tcatgggttt ctataatggt caggctggca tttgctagac cgccatcttt acgaagaagt
      721 ggttctttat agcctaggag agtacgaaga ctggcagtaa gattggcgcc gtgtccgaca
      781 attagaatac gctcagctgg actatctttt aatgatttga taaattggat ggtccgttga
      841 gttgtactat agagggattc ggctccgaac attcgagtgt caaattgagc aagatttgaa
      901 cgaaaagcct ggatttgttg cgggtaaata gcttccaagg ttgcaatttt caaaccttct
      961 aacttcccaa gttgccattc acggagatta ggaacgattt ctaaagaaca gggggtatgg
     1021 agttgacttt ggataatctc agcagatttg accgctcgag gtaaatcact tgaataaatc
     1081 tgatcaaaag gaatttcctt gagatactga ccaagtcgtt ttagggtttc aatggattca
     1141 ggaagaagag gagaatcacc gctagcacct tgaaaacgac cttcttggtt ccagagggta
     1201 cgaccgtggc ggacaaagta gagtttcatt acttgatgtc ctccaaaata tctacaaagt
     1261 ctgcctttac aaagctagcc aagtcttgtg gcgcgacgat aatgctgtgt ccgacttcgc
     1321 ctgcagagac aatcatttga tccaaaccta gagcaatttt atcgataaaa atgggataat
     1381 tgtgtttctg acgaattccg acaggattat tggctccatg aatgtaacca gttgtttttt
     1441 ctaagtcctt ttgtggaatc atgctcactt ttttattgcc agaaatttta gctagtttct
     1501 tttcagacaa gtgctgagtg atagggacaa ttccgataat cggtccggtc ttgtctccca
     1561 aaagcgccaa ggttttgaaa atctgatctc gttcataacc ttgaggaagc tctccttcta
     1621 gggcattgat ttgaatcccc tgatgaggga tagctgcttt agataggatt tgttctacca
     1681 atgttttttt gattttaact ttttttgcca ttatttatat ttatcctcca attgactcat
     1741 ccaaatacca agccagattc ccagcgcaaa gaagaaggcg atgatgacat aaccgacaag
     1801 tgaaagtcct gtgtattgga tactttcagc gtttcctgca tttggaatta agatcaaaag
     1861 ggtacttgat aggacgatac cgatgatgaa atgatagacg cgtgagtggt agttgtttaa
     1921 ggcataatcc atcaattttg aaaaaacgat gagagttgca cctgcaccaa ttccaatcgg
     1981 aaagaaggtt cccaagagat caaaagtttt aaaaccagtc aacataggag catagagtcc
     2041 caaaatcaaa agtaaattcg atgggctgag gccaggaacc aagacgccaa gggccaatag
     2101 tgcgcctgct aggacgaagt taagaaagct ggcgcttaag gttccaacga caaaatttaa
     2161 ggcatagagt cctaatccag aaatgataaa ggttgtccat aaccaagcta aatcaatctt
     2221 gtctcggtca gattctcgag ttgattcttt gaggaggcta ggaactgtac cgataatagc
     2281 tcccgcaaag ctccataata caaagacctg ataattttca agcaggtatt caatcgggta
     2341 ggaaaataag ccgattccca gaagcatacc gatggcaact ggaataaagt acaaaacatt
     2401 ttctttaagg tctttaaagg gatgggccag aaagccaatc attcgttcat agattcctaa
     2461 gattgctgct agaacccctc cggaaattcc tggtaggata aatccaagag caattacaat
     2521 ccctttaata acgcgtgcta accatgagag catatatttc ctccaactat ttctatttca
     2581 aaaaatcctt actatattgt atcacaatca gacacaaaaa gaaaaagcaa atgataaaca
     2641 aatgctttta aggttttaaa aaaagttttc gaaaggtttc ttctttattt tttaagggag
     2701 agataacgtt gatatctaaa tcgtggtcaa agccggcaat ttttccttta gatgtgtatt
     2761 ggtgaatatc ataatctaaa tcagttttag gactgctctc caaaaatcct gagtctgagc
     2821 cgtaggacgg aatccaaaca gaggtaaact tgcctgtatc aatactgtgt tcttccatga
     2881 agtagacacc aacgtagatg ccgatgtttt tagcacctag tgatgctagt tttgctcgaa
     2941 agttttcgac accttcgttc atattagaca tggttttgtc ttccacgtca agccaatagt
     3001 aactagggct gtaaggagag gcagcattgt agaaaacttc ggcagccttt tccatttctt
     3061 gggcactttt tccagctaca taagcgtaga cagcaactgg gacattccgc ttttgaagtt
     3121 cagtgatatg actcttatag gccttgtcta ttccattgat aaatgaagca tcattttctt
     3181 ttgtcgtttg agcaccactg tgaacacgaa caatagcacc tgaaatattt tgtgagaggg
     3241 catcgtagtt gatttcctca ggacgctgcc agccagagag gtcaataatc ggtttgtcta
     3301 agtgtttcaa agcctgtgct tcaatctgtg ctatattgga ttttgtttta aacgattggc
     3361 tgtcattaag tgggcgattg atgattaaaa tgaacatcat aatccccaaa aaactaaata
     3421 aaataagtgg atgaatttgt tttctcacat cttataattc taccctaaaa atcaaaaaaa
     3481 atcaaaaaaa tgggttaagg aagagacttt agagcatttt ttcattcaag agtgcggaat
     3541 gatttgaaat atggtataat aaaagggaat ttctacagaa aagagaagat tatgtcaaat
     3601 tttgccatta ttttagcagc gggtaaaggg actcgcatga aatctgattt gccaaaagtt
     3661 ttgcacaagg ttgcgggtat ttctatgttg gaacatgttt tccgtagtgt gggagctatc
     3721 caacctgaaa agacagtaac agttgtagga cacaaggcag aattggttga ggaggtcttg
     3781 gctggacaga cagaatttgt gactcaatct gaacagttgg gaactggtca tgcagttatg
     3841 atgacagagc ctatcttaga aggtttgtca ggacacacct tggtcattgc aggagatact
     3901 cctttaatca ctggtgaaag cttgaaaaac ttgattgatt tccatatcaa tcataaaaat
     3961 gtggccacta tcttgactgc tgaaacggat aatccttttg gctatggacg aattgttcgt
     4021 aatgacaatg ctgaggttct tcgtattgtt gagcagaagg atgctacaga ttttgaaaag
     4081 caaatcaagg aaatcaacac tggaacatac gtctttgaca acgagcgttt gtttgaggct
     4141 ttgaaaaata tcaataccaa taacgctcaa ggcgaatact atattacaga cgtcattggt
     4201 attttccgtg aaactggtga aaaagttggc gcttatactt tgaaagattt tgatgaaagt
     4261 cttggggtaa atgaccgtgt ggcgcttgcg acagctgagt cagttatgcg tcgtcgcatc
     4321 aatcataaac acatggtcaa cggtgttagc tttgtcaatc cagaagcaac ttatatcgat
     4381 attgatgttg agattgctcc ggaagttcaa atcgaagcca atgttacctt gaaagggcaa
     4441 acgaaaattg gtgctgagac tgttttgaca aacggtactt atgtagtgga cagcactatc
     4501 ggagcaggag cggtcattac caattctatg attgaggaaa gtagtgttgc agacggtgtg
     4561 acagtcggtc cttatgctca cattcgtcca aattcaagtc tgggtgccca agttcatatt
     4621 ggtaactttg ttgaggtgaa aggatcttca atcggtgaga ataccaaggc tggtcatttg
     4681 acttatatcg gaaactgtga agtgggaagc aacgttaatt tcggtgctgg aactattaca
     4741 gtcaattatg acggcaaaaa caaatacaag acagtcattg gagacaatgt ctttgttggt
     4801 tcaaattcaa ccattattgc accagtagaa cttggtgaca attccctcgt tggtgctggt
     4861 tcaactatta ctaaagacgt gccagcagat gctattgcta ttggtcgcgg tcgtcagatc
     4921 aataaagacg aatatgcaac acgtcttcct catcatccta agaaccagta ggagcctatc
     4981 atggagtttg aagaaaaaac gcttagccga aaagaaatct atcaaggacc aatatttaaa
     5041 ctggtccaag atcaggttga attaccagaa ggcaagggaa ctgcccaacg ggatttgatt
     5101 ttccacaatg gggctgtctg tgttttagca gtaacggatg aacaaaaact tatcttggtc
     5161 aagcagtacc gcaaagctat cgaggctgtc tcttacgaaa ttccagccgg aaaattggaa
     5221 gtaggagaaa acacagcccc tgtggcagct gcccttcgtg aattagagga agaaacagcc
     5281 tatacaggga aattagaact cttgtacgat ttttattcag ctattggctt ttgtaatgag
     5341 aagttaaaac tatatttagc aagcgatttg acaaaagtgg aaaatccgcg tccgcaggat
     5401 gaggatgaaa ccttggaagt ccttgaagtg agcttagaag aagcgaaaga attaatccaa
     5461 tcaggtcata tttgtgatgc caagacaatt atggctgttc agtattggga gttgaagaaa
     5521 aaatagagga ggtcagtatg ggtaaatctt tattaacgga tgaaatgatt gaaagagcta
     5581 atagaggcga aaaaatttca ggtcctcctt tgctagatga taatgaggag actaagattt
     5641 taccaacctc ttcttcccgt tttggttatg ccaatcctaa gaatcatggt tttagccagg
     5701 aaaccttgaa gattcaggtc gaaccatcta ttcataaaag ccgtcgcatt gaaaatacca
     5761 agagaaatgt cttcaattct aagttgaata aaatcttatt cgcggtcatc tttctcttga
     5821 ttttgcttgt tttagcaatg aaacttttgt aatagaaaag gaattgaaat gaaaatagga
     5881 attattgctg ctatgccaga agaactggct tatctggtcc agcatttaga taatgcccag
     5941 gagcaagttg tttttgggaa tacctatcat acaggaacca ttgcttctca tgaagtcgtt
     6001 cttgtagaaa gtggaattgg taaggtcatg tctgctatga gtgtggcgat tttggctgat
     6061 catttccagg tggatgccct tattaatacg ggttcagctg gggcagtagc agaaggtatc
     6121 gctgttgggg atgtcgtgat tgctgacaaa ttagcctatc atgacgtgga tgtcacagct
     6181 tttggctatg cttatggaca aatggcgcaa caaccgcttt atttcgaatc agacaaaacc
     6241 tttgttgctc aaatccaaaa gagtttatct caattggacc aaaactggca tcttggtttg
     6301 attgctacag gagatagttt tgttgcagga aatgacaaga tagaagcgat taagtcccat
     6361 ttcccagaag ttttagccgt ggagatggag ggggcagcta ttgctcaagc agcgcatgcc
     6421 ctcaatctcc cagtcttagt catccgagct atgagtgaca atgccaacca tgaagcaaac
     6481 atcttttttg atgagtttat tatcgaagct ggacgtcgct ctgcccaagt cttgttgacc
     6541 tttttgaagg ctttagatta agcggaaatt tgacagtttt tctagcttat gataagattt
     6601 aagtaaagaa aagctagaaa acgtttcaga ggatattatg agtattgaaa tgaccgtcag
     6661 tgagattgca gaggtcttag gattatctcg ccaagcaatc aataaccgtg tcaaagaatt
     6721 accagaagaa gacacagata aaaatgacaa aggggtaaca gttgttacca gaagtggctt
     6781 gattaagcta gaagaaatct ataaaaaaac gatttttgaa gatgaacctg tcagtgaaga
     6841 tgtcaaacaa cgtgaactga tggagattct agtggatgag aagaatgcag aaattcttcg
     6901 tctttatgaa caattaaaag ccaaggatcg tcagttatca gaaaaagacg agcagatgcg
     6961 tatcaaagac cgtcagattg ctgagaaaga caaacaatta gatcagcaac aacaattgac
     7021 ccttcaagcc atgaaggatc aagaaaatct taagctagag ctggaccaag caaaagaaga
     7081 agtccaatcc actaaaaaag gcttttttgc tcgtttattt ggaggataat caaggagctg
     7141 tttaggttaa atgctaacct gatggcttct tttgtttgtg aatagtatag ttgattagaa
     7201 cagagacagt agaggagagg tacagaatgg aacgattaga agattacatt gcttttgatt
     7261 tagaatttaa tcagcacgaa ggacaaacac acttgattca agtatcggca gtgcgtttta
     7321 gagatggtcg ggaaatcgat gcctatgatt cctatgttca taccagcgtc cctctaaaga
     7381 gctttatcaa tggtttgacc gggattacag ctgagacctt aaaagacgct cctccagttg
     7441 agaaagtctt agaagaattc caagagtttg tgggttcttt gctgatgata ggctacaatg
     7501 ctgctaaaag tgatttacct attttagcag agcatggttt agactatagc caacagtaca
     7561 aggtggattt gtatgatgaa gcttttgaac gtcggagttc agatttacat ggtattgcca
     7621 atctcaaatt gcaaactgtt gcctcatttc ttggatttca aggtcagtct cataatagtt
     7681 tagaggatgc tcggatgacg gcgcgtgttt acgaatcctt cttagagtcg gatcaagcta
     7741 gggaattgtt agagacagaa agtagcttat caaacaatcc ttttggtggc ttggacttgt
     7801 ctcaattatt tgactaggag tgcctatgaa acctgaaaaa cctaaaaaat tgggtcttaa
     7861 acagatttac ctcaatcttg ataaaatcct ctttctattt ttcttgattt ttatgatagt
     7921 ggagtatttg tgggtaccat tcaattcatg gttggctagc ttcttattag atcagacagg
     7981 ttatcttttt atttcctata acaatgtctt agccatcctt actcattctc cattgattag
     8041 tctagctttt ttagccttga ttgtaattaa ccttctggtt gcttattttc aaataggact
     8101 cctctttatt ggtgctcgtc atctcctcta taatgaaaag cgaaggctaa ttgagtatat
     8161 ccgcaaggtt ttccatgaaa gttttggatt tatgaaaaag ctaacttgcg ctaaagcctt
     8221 gtttatcttt ttctatatag ctatgctgtt tccttttata cggaaaatgc tgaagattta
     8281 ttatcttaat aaaatcgtca ttccagagtt tatccaagct tatttagaag atagatattg
     8341 gatgtggtgg ctgagcatcc tacttctatc tcttatcttt ctctatgtct ctgtcagatt
     8401 gatgtttgcc cttccgaaga ttgtatatga tcagctgact gttcgagaag cagtgatgtt
     8461 tagcttggag aaaaccaaga agagagttac tttttatgct tggaatttat tttacatctt
     8521 gctcaaagca catttattat tttatcttcc tttgattcct ttattattgg ctcaaacttt
     8581 ggtagatgat atcactcaga gagagtctct gattcttggt attttgaatt ttgtcgtgat
     8641 taaaaatctc tattatatgg ctctgactta ttttttggtt aaatttgttt catttttgac
     8701 aggaaaggag ctggatatgc ttcctaggag agaaaaagat cacatcgtgc gatggggtgt
     8761 catgacttgt gccagtcttt tctttgcctt agaaggttat atttatctgg aggctcccat
     8821 ggttcatcta cctcaactta tttctcaccg aggggtttcc aatgcaaatg ggattcaaaa
     8881 tacagtagag tccttggaaa ctacagcaca actcaaacca gacttggtgg agacggacgt
     8941 gcaggaaaca aaagatgggc agtttgtcat gatgcatgat gctaacttga aaaatctagc
     9001 aggtatcaat aaaagtcctc aagacttaaa cttggaggag cttaaaggga ttgatatttt
     9061 tgaaaatggc taccagacta aaatttcaag ctttgaagat tatctcagtc gagccaacga
     9121 acttggtcaa aaattactaa ttgaaattaa aaccagtaaa aaagatagtc cagacatgat
     9181 gaaccgcttt ttagcccgtt atgctgcaat gctcaagatt tatggacatc aaatccagtc
     9241 tttagactac catgttgtcg aaaaagtaag acagtatgat gccgaactgc cagtttattt
     9301 catcatgccc tacaattctg tctttcctaa aacaagagcg acaggatata ctatggagta
     9361 ctcaaccttg gatgaatatt ttgtaagcaa actatggaca acggatcaga aactttatgt
     9421 ctggactgtc aatgattcag aagctatcag caaatctctt cacttaggag tagatggagt
     9481 gattacagat gatttggaaa aagttcagcg ggaaatagaa gtagcccaag aagatccaga
     9541 atatacggat ttgctcttga aaaaagcatt ggaattcttt gaattttagt aataaaaaag
     9601 aggaacggtt gttcctccca tgataggtaa gtattcttgt gaatgcttgc ttttttgtgg
     9661 ttgcaatccg tttacgagaa ttgtttatat tgttcttgtt tcaatttact ttatagtgct
     9721 ttgaagttgg aatgctacac tataacttat aaaatatagt aaaatgaacc aaaaatagta
     9781 cacaatgtgg tataatcttc ttatgg
//
LOCUS       DAVKJU010000068         9718 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVKJU010000068 DAVKJU010000000
VERSION     DAVKJU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.068"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..98
                     /locus_tag="VW191_001736"
     CDS             <1..98
                     /locus_tag="VW191_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEU9624466.1"
                     /translation="SSTVLPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            64..378
                     /locus_tag="VW191_001737"
     CDS             64..378
                     /locus_tag="VW191_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624467.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            1062..1223
                     /locus_tag="VW191_001738"
     CDS             1062..1223
                     /locus_tag="VW191_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624468.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1315..2994
                     /locus_tag="VW191_001739"
     CDS             1315..2994
                     /locus_tag="VW191_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEU9624469.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGYKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3262..3828
                     /locus_tag="VW191_001740"
     CDS             3262..3828
                     /locus_tag="VW191_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624470.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            4150..4338
                     /locus_tag="VW191_001741"
     CDS             4150..4338
                     /locus_tag="VW191_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEU9624471.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            4400..5158
                     /locus_tag="VW191_001742"
     CDS             4400..5158
                     /locus_tag="VW191_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU9624472.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            5216..5707
                     /locus_tag="VW191_001743"
     CDS             5216..5707
                     /locus_tag="VW191_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9624473.1"
                     /translation="MLGVIIGDIVGSVYEWNKIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYE"
     gene            5711..6070
                     /locus_tag="VW191_001744"
     CDS             5711..6070
                     /locus_tag="VW191_001744"
                     /inference="COORDINATES: protein motif:HMM:NF015692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEU9624474.1"
                     /translation="MNDNPIECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQET
                     VPQAIIAFLESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLD
                     EPLKDVVRRWENRIKAY"
     gene            6182..7066
                     /locus_tag="VW191_001745"
     CDS             6182..7066
                     /locus_tag="VW191_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEU9624475.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASRERYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKFLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            7229..8968
                     /locus_tag="VW191_001746"
                     /pseudo
     CDS             7229..8968
                     /locus_tag="VW191_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
     gene            9243..9698
                     /locus_tag="VW191_001747"
     CDS             9243..9698
                     /locus_tag="VW191_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624476.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
BASE COUNT         3477 a         1347 c         2036 g         2858 t
ORIGIN      
        1 tatctagcac agtattgcca gatatcaaaa ttaaattacc gatagacagt agaggtactc
       61 cagattggaa ttacatggaa agatatagag atagataaag agagagaggg agagatagat
      121 aaaaagtcca cccccatttt ttatggagaa ttcaaaaatg taaggttaag caaagaagaa
      181 tataaaaatc taaaagaaaa attaaactca cacacagata taatgataaa taaactatcc
      241 agatacatgg aaagcagtgg taagacctat caaaaccact atgtgacaat cttaaaatgg
      301 tatgaagaag ataaagacaa actaagacag aaaggtttaa ataaaaaaat gaattatgat
      361 gtaggagaat ctttatagat aaaaagaggt ggaaaggcac tattaaagac tttaagtcta
      421 aaaaggtatc aaagtatgcc agagataaaa aaatataaat atgacacttt aaaggcgatt
      481 agaaaattaa agaaatgcta gaagactttt agatgacagt taccaagagg gtctaatttt
      541 cactgctact tatgcagtgg gaattggacc tttttatata agcaaattct atttcaaaat
      601 tggttaccaa gaaagacttc ttttcactgt taagtataag agggtaattt atagaatttt
      661 gttacatcaa gataaatatt ttctgatggt caaattttaa aaagattaat aactctcgat
      721 aaattttgaa ccttaacaat tgaatagacc aagacaagac gttaattatt tttggagcgt
      781 aataacctat ccgtcaagat ctttcggctg aataattcgg caaaaaaagt tctgctaagc
      841 aaaaagaaag tgagcgataa gtaagagttt gaatataggg agggataccc tatccgctat
      901 gaagaaaatg aggataatga tacttctaag gttgaagccg gctcatccgg aatagaggcg
      961 gaggtgagat tcctatgttg ctaatccaaa gcacttgtca atgtcaaaaa acttaattaa
     1021 acaaataatt attagatata aaactttaga ggaggtatat attggacaat gactggaatg
     1081 gattggctga tttaatagca aatctgattg caaaatatgc tggtgcttta gatttagata
     1141 atctacccga tccaacgcca gcaaaaaatc aagagatgaa aaatagtttt gacatggcaa
     1201 aaacacaaat tgagacggac taataaaagt gttataatat acttgaaatg atagtccaaa
     1261 cttaaattaa aaaaattata tatgatatag aaaaattata ttgaggttaa tactatgtca
     1321 aaagaaaaaa taaaagtata cctctataca cgagtatcta cgtcaataca gatagagggg
     1381 tattcgttag aggcacaaaa atcacgaatg aaagcttttg ctatttacaa cgattatgaa
     1441 attgttggag agtatgaaga tgccgggaag tctggcaagt ctattgaagg tagaatacag
     1501 ttcaatcgaa tgatggaaga tataaaatct ggaaaagatg gagtatcctt tgttcttgtg
     1561 tttaagttat caagatttgc aagaaatgcc gctgatgttc tttctacact tcagataatg
     1621 caggattatg gagttaatct tatctgcgtt gaagatggca ttgattcatc taaagatgct
     1681 gggaaactaa tgatttctgt tttatcagct gtggctgaaa tagaaagaga aaacatccgt
     1741 attcaaacaa tggaaggtcg cattcaaaaa gcaagggaag gaaaatggaa tggtggtttt
     1801 gctccgtatg gatataaact tgaagacggc aagctgttta taaatgagga agaggcagtt
     1861 gcaataagaa cgattttcga ccagtatgta aatactacga taggagccaa tgggatatct
     1921 aaatacttag agaatcatgg aattagaaaa atcccaagac agaatggtaa gaatcctttg
     1981 tttgatgcag gtcttataag aaagatatta aagaatcctg tatataatgg gaaaatagca
     2041 tttggaagaa gaactttaga aaaagttcat ggtacaagaa atgaatataa gcaggttgaa
     2101 caagatgaat atctaatatc tgaagggata catgaagcta tagtttccga tgaagtttgg
     2161 caagctgctc aggttaagct aaaatctcaa gcaaagaaat atgagcatgt gaataaagga
     2221 aaagatacac gcacacactt gctttctgga attgtaaaat gcccgatatg tggagtggga
     2281 atgtttggga acaagtgtat caagaaaaag aaagatggca caaagtataa agatttttat
     2341 tactatggtt ataaacatag gcagatgata agaggtcata agtgtacatt cagtaagcaa
     2401 attagagaag aattgttaga tgatgctgtt gcagaggtga ttgtcaagat agtaagtaat
     2461 ccgaaatttg cttctatgat gcaagaaaaa atcaacatga aggtggatac ctctgaaata
     2521 gaaaaagaaa tagataatta ccaaaaggaa ttgagaaaga gtcattctac aaagtttaag
     2581 ctaattgagg aaatagataa tttagatgtt gaagataagc attataagag aaggaaacag
     2641 gatttagatg atagactata tcgtatgtat gacaaaatag atgaattaga atcatcacta
     2701 attgatgcga aagcaaagaa acagactatt gaagctgaaa agcttacagg agataacata
     2761 tataaggttc tgatctattt tgataagctc tataaagtca tgaatgatgt agagcgtagg
     2821 cagttaattt cagctttgat ttctgaaatt caagtttatg aagaaaaaca atctaacgga
     2881 caatggctaa aatccattac ttttaagcta ccaattatag aagaaaatct caacataggt
     2941 ttggacaatg atgaacaagt tgaatgtgta agtttattag agaaacgtag ttaatcctca
     3001 aaaggttccc caaacctttt gagtcccact gacgcatcac gctgaggcgg tatcactgct
     3061 tgtacgagtt gaagcatcag cgaagtaaaa gtagatgttc agccaatgcg acgaggtgag
     3121 accgaaggtc gataaggtgt gttctgggta taggactcgt aaagtaagta ggaaatatga
     3181 tgagatgata cagagagcct gaattggtca ggtgacgtgc ttatttacat tagcctgata
     3241 atgaaaatgg agagattatc gatggttata ttggaattgt accaaaatga ttattctaaa
     3301 gatttagtag catttgattc catagaagat gggaaagcct ttgtagctca aatcccggga
     3361 tacactctgg aaacagaaga tggttttgaa gtggagtatt ttaatcctaa aaacataccg
     3421 gattatatgg aaattatttt taacggaaat attgttcctt tatctaaatt tatgttcgat
     3481 cctgaggaaa atgtaaacat tatttggaaa gagatttcaa atttatccct caaaaatgat
     3541 agagtgatcg aaggatattc aaaaattgat gcatatgtcg ttaacaacca tgaagttaaa
     3601 gcctatgtcg aaacaagaga aacgaactat cgtaaagcaa aagacttctt agaaagccgc
     3661 ggatatgaaa tcgacagaag ctttttcgga agtgaagacg gtgaagcgat tctttacaga
     3721 aaaaaaggga tcgaagtttg gcatttcttg tgtcacttag acccaatgtt tgtagagatt
     3781 gaagatgtag aaggatatgt taaagaagaa gttggagaga ttcaatagtc aaccaaagtt
     3841 ccttgtgagt atataccttt gctagtaaaa gagtcaaaac gttataacga agggggggaa
     3901 gaaaaccctt gagtgtaagt ctttttcttg tttagaggga aagtattaga gtagccgctc
     3961 ataatttata ctagatttga taactgattt gagacagatt ttatttctat aaatattgtg
     4021 aaaatttttt aataccaaag atttaaagtt actgtgttaa aaatcctctc cacttttaaa
     4081 ctaatgtatt ataaaagcca gttgacacta gtgttacacc tgtgatagaa ttaacttgag
     4141 gtgatgaaaa tggcaaccga taaaaataga ataatgatta gcttggatga taaaaatcta
     4201 gagaaacttg aaaatttagt agaagatgct agagatagaa ggggaatgcg actaacaaag
     4261 tcccaggtta ttgaattact cctaaatacc gttgattatt tcgatgatat tatgggggct
     4321 atttactcaa aaaaataaga gcaaaattgc tattattttt tacaaattgg tgtaacacta
     4381 gtgatatagg agtgatgaaa tggttgttta tattagacaa agtaaattac caagtgaggt
     4441 ttcaattaat aaatacaatg ctcaagtagg tgcctactta caaggagaag aagttatttt
     4501 atatcaatct ttttctgaaa taaaagagtt aacaagtgaa gatatagttg tagattatat
     4561 aatggagact agagcattac taaaaatgat gggcttaaac gttccggttc atgactatcc
     4621 tattgagctt aaagagtttt atggtcgaaa gatttatgct ggtattttag gagagattgt
     4681 gaatatacct gataattggg gaaaatttat taagcctaaa gctggttcaa aagtctttac
     4741 tggaagagtt gttaatggaa cccatgattt aataggtatt ggtctacctt tcgactatcc
     4801 tatatggatt agtgaggttg tagaattcat agctgaatgg cgttgttttg tgttagatgg
     4861 tcgcgtatta gatgttcgac cctatacagg tgattatcat gcacaatttg atgcaagtgt
     4921 aattgatgaa gcgatatcat gttggaaaga tgcgccaata gcttatggac tagatatcgg
     4981 tgttactcgc gatggcagaa cacttgttgt tgaagtaaat gatggttatg cattgggaaa
     5041 ttatggccta tctcctttaa aatcaatcaa ttttcataga gctaggtgga aagaaatggt
     5101 aaaaccctat tttgaaaaaa atgaaatttt taaaatccaa caggatgtta ttttctaaaa
     5161 taaatatggt agataaccaa tataaaaagc atcaaaatga acaaggagga atctgatgct
     5221 tggagtaatt attggagata ttgtcggttc tgtttacgaa tggaacaaaa tcaaaacaaa
     5281 ggactttcct ttatttcgga aggactgctt ttttacagat gacacggtta tgacctgtgc
     5341 tgttgcagaa gcaattatga atggtggaca aaaagacgac ttcattgacg cgatgaagaa
     5401 atatggcaga atgtatccga atgctgatta cggtgctcgg tttaatgcat ggctaaacag
     5461 cgataaccgt gagccttata atagctttgg gaatggatca gctatgcgta tttctccatg
     5521 tgcttggatc atggactgtg gtttttatgc gaaaactggt atatggccat catctagagg
     5581 acttacgagt ctttctgcag aggtaactca taatcatcca gaaggtgtca aaggcgctat
     5641 ggctacagct gatgctatct ttctatgtcg tttttacttt ggtggttatt gtagagagta
     5701 cgagtaatca attaacgaca atcctataga atgtaaaaga cgaattaagg attatataga
     5761 aaaagaatac gattacaatc tatctcaaac tttagatgaa atccgtccta actatcgttt
     5821 taacgaaaca tgtcaggaaa ctgtccctca agccattatc gcctttcttg aaagtagaga
     5881 cttcgaagat gcgataagaa atgccatctc acttggtggc gacagtgata ctctcgcagc
     5941 aatcacttgt agcatagcag aggcagctta cggtattcct gattggatca aggataaggc
     6001 ctattcttac ttggatgaac ccttaaagga tgtagttagg cgatgggaaa atagaataaa
     6061 agcgtattaa tctccagtac gtcagatttc tcttttctgt attttcattg tataatagga
     6121 ttacatttac ttgcatacga cgaaatgtaa acatttctat ttaaaattaa ggataaaatc
     6181 aatgaaaaaa gcaatggtaa ttatcaaccc tacttctggt ggcgagaagg ctttggatta
     6241 caaagaaaag ctggagaata aagcaaaaga atactttgaa tatgttgaaa ccaaaattac
     6301 cgaaaaagcg ctggatgcaa cacattttgc tgaagaagct tctcgtgagc ggtatgatgc
     6361 agtggttgtg tttggtggag atggaactgt caatgaagtc atttcaggta ttgatgagag
     6421 agactacatt cctaagttag ggattatccc aggcggtacg ggtaacctca ttacaaaatt
     6481 tttggaaatc aatcaagaca tcgatggcgc aattgacgaa ctggattttg atttaaccaa
     6541 taagattgat atcggtaaag caaatgacaa ctattttggt tatatcttta gtatcggttc
     6601 tctgcctgag gcgattcaca atgttgaaat agaggacaaa acaaaattcg gtattttaac
     6661 ctatgctgta aataccatga agtctgtcat gacagatcag gtctttaaca ttaaggttga
     6721 gacagaaaat ggaaattatg ttggtgaagc tagccatgtt ttggttcttt tgacaaatta
     6781 cttcgctgat aagaaaatct ttgaagaaaa caaggacggc tatgccaaca ttttgattct
     6841 gaaagatgcc tctatattct ccaaattatc cgtcattcct gatttattaa aaggggatgt
     6901 tgtcgcaaat gataatatcg agtatatcaa agcgcgtaat attaaaatct cttcagatag
     6961 tgaattggag tcagatgttg acggagataa atcagataac ctacctgtag aaatcaaagt
     7021 cctagctcag cgagtagaag tattttcaaa accgaaagag gattagtata tagagaaagc
     7081 cttttttaag gctttttgta tactttaaaa gatagttcct ttaacaacgg acattccttg
     7141 caaatagttt acaaaaatag tatactggat ttattgagtt tgaaaacgtt tgcgtaaaat
     7201 ttgaatgaat actttaggag acaaattgat ggaattaagt gctatttacc ataggcctga
     7261 gtcggagtat gcctatcttt ataaggataa gaaactccat attcgaattc gaactaagaa
     7321 aggggacatt gaaagcatca acttgcacta tagggaccct tttatcttta tggaggagtt
     7381 ttatcaggat acaaaagaaa tggtcaagat aacttctggt accttatttg accattggca
     7441 ggttgaagtg tcagttgact ttgcacgtat ccagtatctc tttgagctca gagatacaga
     7501 aggtcaaaat attttgtatg gcgataaagg gtgtgtggaa aattctctag aaaatcttca
     7561 tgcaattggg aatggattta agttgcctta tcttcatgag attgatgcct gcaaggttcc
     7621 tgactgggtt tcaaatacgg tatggtatca gatatttcct gaaagatttg ccaatggcaa
     7681 tgctctatta aacccagaag ggactttaga ctgggattca tctgtcacac ctaagagcga
     7741 tgatttcttt ggtggtgatt tacaggggat tattgatcat atggattact tgcaagactt
     7801 gggtattact ggactatatc tttgtcccat ctttgaatct acaagcaatc acaagtacaa
     7861 tacgacagat tactttgaaa ttgaccgtca ttttggagac aaggagacct ttcgggaact
     7921 ggtggatcaa gcgcatcatc gtggcatgaa agtcatgctg gatgcggtat ttaatcatat
     7981 tggttcgcaa tctcttcaat ggaaaaatgt cgtcaaaaat ggtgaacagt ctgcttataa
     8041 ggattggttc catattcaac aattcccagt gacaactgaa aagctagtta ataagagaga
     8101 cttaccctat catgtttttg gtttcgagga ctatatgcct aagctaaata cagccaatcc
     8161 agaggtcaag aattatcttt taaaggttgc gacttattgg attgaagagt ttaatatcga
     8221 tgcttggcgt ttggatgtgg ctagtgagat tgaccatcag ttctggaagg attttcgtaa
     8281 ggcagtttta gctaaaaatc ctgatcttta tatcctagga gaagtctggc atacatctca
     8341 gccttggcta aatggagatg agttccatgc cgtcatgaat tatcctttat ctgatagtat
     8401 caaggactat ttcttacgag gaattaagaa gacagaccag ttcatcgatg aaatcaattg
     8461 agagtctatg tattacaagc agcagatttc agaggtcatg tttaatctct tggattcaca
     8521 tgatacagag cgaatcctgt ggacggccaa tgaagatgtt caactggtta aatcagcctt
     8581 agcctttctc ttttaacaaa aaggaacacc gtgcatttat tacggaaccg agctagcctt
     8641 gactggagaa ccagatccag attgtcgtcg ttgtatgcct tgggaacgtg tatcaagtga
     8701 caatgatatg ctgaacttta tgaagaggct gattaagatt cggaaatacg cgtcagtaat
     8761 catttcgcat ggcaagtata gccttcaaga aatcaactct gatctagtag ctctggaatg
     8821 gaaatacgaa ggacggatcc tcaaagcaat attcaaccaa tcaacagaag attatctttt
     8881 agagaaagaa gcagtagcac tagcaagcaa ttgccaagaa ttggagaatc agcttgtcat
     8941 ctctccagat ggatttgtga ttttctaaaa actagttgat gaagattatg gtacatttca
     9001 tatcttatat agtataataa ggctagttac taaacttgta aaggagaact taaatgaatt
     9061 gtagaggaca tgaaacaaga caaagaattg ttagagattt tgaagtttag cctaaagcac
     9121 atattaagct gttagcaaat caacaaaaac atagtgatgc aggagcaact attgaagatg
     9181 aatattatgt atttatcgct gagagtaaaa ttgatggcaa gaaggaagtt attcagtgtt
     9241 gcatgggtgc ggcaagggat tttttagaac taattaatca caaagggcta cctcttttta
     9301 atccgcttgt aggtgattct catgtaaata atagacaaga atatgacaat acagggagtg
     9361 gaaatttaca acctgaaaag tggaatgaaa ctgcaaagca gctttataat gttataatgt
     9421 ggttgattat tttatggaat gctaagccgg atacaccttt atttaatttt aaagacgaag
     9481 taattaagta taaaacatat gagccttttg aaagcagtat aaaaagagta aatactacta
     9541 taaagaatgg tagtaaaggg aaaactctga ctgagatgat taatggctac agagcggata
     9601 acgatattag agatgaaatt tgtaacttta atattctgaa aaataaaatt cgtgatatga
     9661 aagaccaaca aggaaataca atggaatctt acttttagtt attgttgaat tttgggta
//
LOCUS       DAVKJU010000069         9636 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVKJU010000069 DAVKJU010000000
VERSION     DAVKJU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.069"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..201
                     /locus_tag="VW191_001748"
     CDS             <1..201
                     /locus_tag="VW191_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HEU9624477.1"
                     /translation="NANGSMATGWLKDGDTWYYLEASGAMKESQWFKVSDKWYYVNGS
                     GALAVNTTVGGYRVNANGKWVN"
     gene            complement(431..1411)
                     /gene="dusB"
                     /locus_tag="VW191_001749"
     CDS             complement(431..1411)
                     /gene="dusB"
                     /locus_tag="VW191_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU9624478.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1398..2270)
                     /gene="hslO"
                     /locus_tag="VW191_001750"
     CDS             complement(1398..2270)
                     /gene="hslO"
                     /locus_tag="VW191_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU9624479.1"
                     /translation="MNKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2435..3862
                     /locus_tag="VW191_001751"
     CDS             2435..3862
                     /locus_tag="VW191_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9624480.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSENENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            4021..5529
                     /gene="glpK"
                     /locus_tag="VW191_001752"
     CDS             4021..5529
                     /gene="glpK"
                     /locus_tag="VW191_001752"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU9624481.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5571..7397
                     /gene="glpO"
                     /locus_tag="VW191_001753"
     CDS             5571..7397
                     /gene="glpO"
                     /locus_tag="VW191_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEU9624482.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPAANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7467..8171
                     /locus_tag="VW191_001754"
     CDS             7467..8171
                     /locus_tag="VW191_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEU9624483.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            complement(8621..9229)
                     /locus_tag="VW191_001755"
     CDS             complement(8621..9229)
                     /locus_tag="VW191_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624484.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2768 a         1897 c         2193 g         2778 t
ORIGIN      
        1 aatgctaacg gttcgatggc aacaggttgg ctgaaagatg gagatacatg gtactatctt
       61 gaagcatcag gggctatgaa agaaagccaa tggttcaaag tatcagacaa atggtactat
      121 gtcaatggct caggtgccct tgcagtcaac acaactgtag gtggctatag agtcaatgcc
      181 aatggtaaat gggtaaacta aacctaatgt aactcgttaa tgctggcttc ctgtaagaac
      241 tctttaaagt attccctaca aataccatat cctttcagta gatactatac ccttgtagga
      301 agtttagatt aaaaaataac tctgtaatct ctagccggat ttatggcgct agagactacg
      361 gagttttttt gatgaggaaa gaatggcggc actcaagaga ctctttaaga gagttacggg
      421 ttttaaacta ttaagccttc tccaattgca agagagcttc aatctctgcc agggtgctag
      481 cttgtgaaat ggctccacgg agtttggcag cgccagatgt tccacggaga tagtgaggag
      541 cgaggccgcg gaattcacga acggcgacgt tttctccttt gaggttaatc aatcgtttca
      601 agtgttcgta ggcgatcttc atcttgtctt caaaggtcaa atcaggtagg atttctcctg
      661 tttcaaagta atggttgatt tggttgaaga ggtaaggatt tcccatggca gctcggccaa
      721 tcatgactgc gtcagcacca acttcttcga tgcgttgctt ggcttcttgg acagtacgga
      781 tatcaccgtt ggcgatgaat ggaatcttgg ttagagcttg ggcaaccttg taaagggtct
      841 caaggtctgc gtggccagta tacatttgtt cacgggtacg gccatgcatg gcgagggcag
      901 aaacacctgc agcttcagca gcgagggcat tttctactgc cagagatggg tccgcccatc
      961 cggtacgcat tttgacagta agggggatat caaggacaga ctgaaccttg ttgatgatag
     1021 agtagatctt gtcaggatcc ttgagccaca tagctccagc ttcgttcttc acgattttgt
     1081 tgacagggca gcccatgttg atatcgacga tatcggtctt ggtgttttct tggatgaatt
     1141 ctgctgcgcg tgctaggctg tcttcatcgc taccaaaaag ttggatagag acagggtttt
     1201 cgccctcatc gatatgaagc atgtgcaggg ttttttcgtt gttgtattgg attcctttgt
     1261 cagagaccat ttccattaca acgagtccag ctccgagctc ttttgcgatg gtacgaaagg
     1321 ctgagttggt cacgccagcc ataggcgcta aaacggtacg attgggaatc tcaacattgc
     1381 caatcataaa aggtgtatta agatttgtca cgaatgagtt cctccaggtc cttttcatca
     1441 aagttgtaag tagtttggca gaattgacaa gtgatttctg ccccgtggtc ttcttctttc
     1501 atttcctgta agtctgagct tggaaggctg gcaagagcgt tcataaagcg ttcatggcta
     1561 cagtcacatt ggaaacggat ttcttcttca gaaagacgct tgtaggcttc gtccccgtag
     1621 atagccttga ggagggcttc gatatggtcg tcgctttcga gaagagtaga gatagctggc
     1681 atttcttgga tgcgtttttc aaagcgagca atctcttctt tcttggctcc tggcaagact
     1741 tgaactagga aaccacctgc aaccttgacc ttgtcttcct cgtccaaaag gacattgagg
     1801 ccgaccgctg aaggcgtttg ttggctttca gtaaggtaaa aggcaaggtc ttcaccgatt
     1861 tctccggaga tgaggggagt tatagagttg taaggatttc cagtaccgta gtctgtgata
     1921 acgaggaatt gaccatttcc aacaaaaggt ccgactagga cttcaccagt cgcagtcttt
     1981 ttgatgtcaa caccaggatt ttgaacgtag cctttgacgt tccccttggt atcagcgacg
     2041 gtgataatag cacctagaga gctagatccc aacaccttaa ctgtaagttt ggtatttcct
     2101 ttttcattgg ctgcgagaat ctggctagcg ataagagttc gaccaagcgc tacagttgag
     2161 ctagcttggg tttggtgttt ttcttgagca gtgcggacgg tttccgtgct gtcaaggaca
     2221 aaagcacgaa aggctccgct ttctgatata gttttaataa ttttattcat agctactatt
     2281 ttagcataaa aatgcccaga gggggagccg tgtgtttact gattttcagg ataatggacc
     2341 aggaaatcag catgaaaata aaaagagaaa cagattattt cagcatttgt aagatttatg
     2401 ctatgcttaa ggtagaaaat gaaaggggta acaaatgtat ttaggagatt tgatggagaa
     2461 agctgagtgt ggtcaatttt caatcctttc ctttctatta caagagtctc agacgaccgt
     2521 caaggctgta atggaagaaa caggattttc aaaagcaacc ctaaccaaat atgtcaccct
     2581 gctcaatgac aaggctttgg atagtggctt agagctgact attcactcag aaaatgaaaa
     2641 tttgcgtctg tctatcggtg cagctaccaa ggggagagat attcggagct tatttttgga
     2701 gagtgctgtt aaataccaga ttttggttta tcttctctac caccaacagt ttttagccca
     2761 tcagctggct caagaattgg tgattagcga ggctacgctt ggtcgtcact tggctggttt
     2821 aaatcagatt ctgtcagaat ttgatttatc catccaaaat ggacgttggc gaggtccaga
     2881 gcatcagatt cgctatttct atttctgtct tttccgaaag gtctggtcga gtcaggaatg
     2941 ggaaggtcac atgcagaaac cagagagaaa acaggagatt gccaatttag aggaaatctg
     3001 cggtgcaagt ttgtctgtgg ggcagaaatt ggacttggtt ctctgggctc acatcagtca
     3061 acaacgtctt cgggtcaatg cttgtcagtt tcaagtcata gaagagaaaa tgcgagggta
     3121 ttttgacaat atcttttatc ttcgtttgct gagaaaggtt ccgtcctttt ttgctgggca
     3181 acatattcca ctaggagttg aggatggtga gatgatgata ttcttctctt ttctcctatc
     3241 tcatcgcatt cttcctcttc atactatgga gtatattctt ggttttggag ggcagttggc
     3301 agatttactg acgcaattga ttcaagaaat gaagaaggag gaactattgg gggattatac
     3361 agaggaccat gtcacctatg aactcagtca gctttgtgct caagtctatc tctataaggg
     3421 ctatatttta caggatcgct acaagtacca gttagagaat cgtcatccat atttactgat
     3481 ggaacatgat tttaaagaga cagcagagga gatttttcat gctctaccta cttttcaaca
     3541 ggggacagat ttagataaga agattctctg ggaatggctc cagttaatcg aatatatggc
     3601 tgaaaacggt ggccagcata tgcggattgg tctggatttg acatctggtt ttcttgtctt
     3661 ttcaaggatg gcagccattt tgaaacggta tttggaatac aatcgtttta ttaccattga
     3721 agcttatgac cctagtcggc attatgattt gctggttacc aataacccga ttcataagaa
     3781 ggaacagaca ccagtctatt atttaaaaaa tgacttggat atggaggatt tggtagcgat
     3841 tcgccagtta ttattcactt aaaaggcttg gttaatccag gtcttttttg tgaaattcac
     3901 acaatctcct cacatttttt taaaaattaa aaaaagttga taaacaagaa agcgctttat
     3961 tttgtatact agtaagtgta aagaggaaac acctcaagat ctttatcagg aggacagtac
     4021 atgtcacaag aaaaatacat catggccatt gaccagggaa ctacaagttc tcgtgccatc
     4081 attttcaaca aaaaagggga aaaggttagc tcgagtcaaa aagagtttac ccagattttc
     4141 cctcaggcag gttgggttga gcacaatgcc aatgaaattt ggaactctgt tcagtcagtt
     4201 attgcgggtg ctttcatcga aagtggtgtc aagccaaatc aaatcgaggc aatcgggatt
     4261 accaaccaac gtgaaacaac ggttgtctgg gataagaaaa caggacttcc tatctacaat
     4321 gctatcgttt ggcagtcacg ccagacagca cctttggctg agcaactaaa aagccaaggt
     4381 tatgtggaaa aattccatga aaagactggt ttgattattg atgcttactt ctctgctacc
     4441 aaggttcgtt ggattttgga tcatgtagaa ggtgctcaag agcgagcaga aaaaggggaa
     4501 ttgctctttg gtactatcga tacttggttg gtttggaaat tgactgacgg tgcggctcac
     4561 gtgactgact actcaaatgc agctcgtacc atgctttata acattaaaga actcaaatgg
     4621 gatgatgaga ttttggaaat ccttaacatt ccgaaggcta tacttccaga agttcgttct
     4681 aactccgaaa tctacggcaa gacagctcca ttccatttct acggtggaga ggtgccaatc
     4741 tcaggtatgg ctggggacca acaagcagcc ctctttggac agttggcttt tgagccaggt
     4801 atggttaaga atacttatgg aacaggctct ttcatcatca tgaatactgg ggaagagatg
     4861 cagttgtctg aaaacaacct cttgacaacc attggttacg gaatcaacgg taaggtttat
     4921 tatgccttgg aaggttctat cttcatcgca ggaagtgcta ttcagtggct tcgtgacggt
     4981 cttcgcatgg ttgaaaattc accagaatct gaaaaatacg ctcgtgattc tcacaacaac
     5041 gatgaagttt atgtcgttcc agcctttaca ggtctaggcg ctccatactg gaaccaaaat
     5101 gctcgtggtt ccgtctttgg tttgactcgt ggaacaagca aagaagactt tatcaaggcg
     5161 actttgcaat ctattgctta tcaagtgcgt gatatcatcg acaccatgca agtggatact
     5221 cagaccgcca ttcaagtact gaaggtggat ggtggtgcag ccatgaacaa cttcctcatg
     5281 cagttccagg cggatatttt aggcattgac attgcacgtg ctaaaaacct ggaaacaaca
     5341 gctctaggag cggccttcct agcaggtttg tcagtagggt actggaaaga cttggacgag
     5401 ttgaaactct tgaacgagac aggagaactc tttgagccat ctatgaacga atctcgcaag
     5461 gaacaactct acaagggctg gaagaaggct gtgaaagcaa ctcaagtctt tgcggaagta
     5521 gacgactaat actggcagaa taaagcgatt tatttagaaa gtgtgtaaat atggaatttt
     5581 caaagaaaac acgtgaattg tcaattaaaa aaatgcagga acgtaccctg gacctcttga
     5641 ttatcggtgg aggaatcaca ggagctggtg tagccttgca ggcggcagct agcggtcttg
     5701 agactggttt gattgaaatg caagactttg cagaaggaac atctagtcgt tcaacaaaat
     5761 tggttcacgg aggacttcgt tacctcaaac aatttgacgt agaagtggtc tcagatacgg
     5821 tttctgaacg tgcagtggtt caacaaatcg ctccacacat tccaaaatca gatccaatgc
     5881 tcttaccagt ttacgatgaa gatggagcaa cctttagcct cttccgtctt aaagtagcca
     5941 tggacttgta cgacctcttg gcaggtgtta gcaacacacc agctgcgaac aaggttttga
     6001 gcaaggatca agtcttggaa cgccagccaa acttgaagaa ggaaggcttg gtaggaggtg
     6061 gagtgtatct tgacttccgt aacaacgatg cgcgtctcgt gattgaaaac atcaaacgtg
     6121 ccaaccaaga cggtgccctc attgccaacc acgtgaaggc agaaggcttc ctctttgacg
     6181 aaagtggcaa gattacaggt gttgtagctc gtgatctctt gacagaccaa gtgtttgaaa
     6241 tcaaggcccg tctggttatt aatacaacag gtccttggag tgataaagta cgtaatttgt
     6301 ctaataaggg aacgcaattc tcacaaatgc gcccaactaa gggagttcac ttggtagtag
     6361 attcaagcaa aatcaaggtt tcacagccag tttacttcga cacaggtttg ggtgacggtc
     6421 gtatggtctt tgttctccca cgtgaaaaca agacttactt tggtacaact gatacagact
     6481 acacaggtga tttggagcat ccaaaagtaa ctcaagaaga tgtagattat ctacttggca
     6541 ttgtcaacaa ccgcttccca gaatccaaca tcaccattga tgatatcgaa agcagctggg
     6601 caggtcttcg tccattgatt gcagggaata gtgcctctga ctataatggt ggaaataacg
     6661 gtaccatcag tgatgaaagc tttgacaact tgattgcgac tgttgaatct tatctctcca
     6721 aagaaaaaac acgtgaagat gttgagtctg ctgtcagcaa gcttgaaagt agcacatctg
     6781 agaaatattt ggatccatct gcagtttctc gtgggtctag cttggaccgt gatgacaatg
     6841 gtcttttgac tcttgctggc ggtaaaatca cagactaccg taagatggct gaaggagcta
     6901 tggagcgcgt ggttgacatc ctcaaagcag aatttgaccg tagctttaaa ttgatcaatt
     6961 ctaaaactta ccctgtttca ggtggagaat tgaacccagc aaatgtggat tcagaaatcg
     7021 aagcctttgc gcaacttgga gtatcacgtg gtttggatag caaggaagct cactatctgg
     7081 caaatcttta cggttcaaat gcaccgaaag tctttgcact tgctcacagc ttggaacaag
     7141 cgccaggact cagcttggca gatactttgt cccttcacta tgcaatgcgc aacgagttgg
     7201 ctcttagccc agttgacttc cttcttcgtc gtaccaacca tatgctcttt atgcgtgata
     7261 gcttggatag catcgttgag ccagttttgg atgaaatggg acgattctat gactggacag
     7321 aagaagaaaa agcaacttac cgtgctgatg tcgaagcagc tctcgctaac aacgatttag
     7381 cagaattaaa aaattaagaa aaaataaaag aggtggaggg cagcattcct tgtcgctcgt
     7441 cccttctttt taatggagac agaaagatga tgaatgaatt atttggagaa tttctaggga
     7501 ctttaatcct gattcttcta ggaaatggtg ttgttgcagg tgtggttctt cctaaaacca
     7561 agagcaatag ctcaggttgg attgtgatta ctatgggttg ggggattgca gttgcggttg
     7621 cagtatttgt atctggcaag ctcagtccag ctcatttaaa tccagctgtg acaattggtg
     7681 tagctttaaa aggtgacttg ccttgggctt ccgttttgcc ttatatctta gcccagttcg
     7741 caggggctat gctgggtcag attttggttt ggttgcaatt caaacctcac tatgaggcag
     7801 aagaaaatgc aggcaatatc ctggcaacct tcagtactgg accagccatc aaggatactg
     7861 tatcaaactt gattagcgaa atccttggaa cctttgtttt ggtgttgaca atctttgctt
     7921 tgggtcttta cgattttcag gcaggtatcg gaacctttgc agtgggtacc ttgattgtcg
     7981 gtatcggtct atcgctaggt gggacaacag gttatgcctt gaatcctgcg cgtgaccttg
     8041 gacctcgtat catgcacagt atccttccaa tttcaaacaa gggagacgga gactggtctt
     8101 atgcttggat tcctgttgtg ggacctgtta tcggtgcagc cttggccgtg cttgtattct
     8161 cacttttcta gtttatactc ttcgaaaatc aaattcaaac cacgtcagcg tcgccttacc
     8221 gtactcaagt acagcttgcg gctagcttcc tagtttgctc tttgattttc attgagtatt
     8281 agaaaacaat tatgttgata gagcttgggc aagagcccaa tttcagcaaa aaatgaagta
     8341 aatcttctca taataaaacg catcatatca agcacgaaaa ttccacgagg tcaactacag
     8401 tcagaaagct gaacaacaag ccaaaacgcc caaaaaaggc ggcaaaaagc aagcacctgc
     8461 aagcaacgtg ccgaaatggt caaatcctga ttatgtcaac gaattagacc caaaaatcgt
     8521 tgatatgcta gtagaatttc acaagtatta tgcttacaaa ttacttgagc aattaactaa
     8581 aatataaacc ctgcctagat ataaaggcag ggtttatatt ttagaaattc acgtaggttg
     8641 ttacggtttt tacataccca gtatagtttg agtttctata gtattcagtg ataaacttcc
     8701 attttctttg agcaacatgg atataagtac ttgttatgta gtatggatat gggctttgtg
     8761 aatccaagta agactgataa gcttgtatac caaaatatgc tccaccaatt attgcacccc
     8821 atggaccccc caataaagca cctatcctac caatcatata actgattcca gcaccagtca
     8881 tgaagttagc gaatgtgtta gcttgtttat tcccatgtat tgtgttgacg taattccaaa
     8941 cattaggatc gtatgatcta aaagatatat ttaggtcgat ttcattcttt tgataagcca
     9001 tataaaatgc cccattgata tagacgccgt cagcacgtcg ttcaatagtg tctacacttc
     9061 catctggatt gacaacctca agaacttcat cgcttaaaat atttacttgc gtatctccga
     9121 accgcactga tgagccattc tcaaactgag cctcaccaga tacaacttta gagtttgccg
     9181 ataagctatc atcagcaaaa acaacaagcg acggggaaat gctagacata cagaaaacag
     9241 acataactag caaacacatg catttaaaca tcttagacat aacggaaact cttttgtatt
     9301 tttgattttt ttcaactttt attatacaat aaaaccaaat aaaaagaaag cggtaacaat
     9361 atgcttaatg cgaaaatttt ttatatattt ttatgtttga tcgttatcga aactacaggc
     9421 ttgttgttgt tgaaaagagg tctcgaaatg ggttatttag acacagaagc tattatcctc
     9481 gcagtttttt catttgcttt ttacaaccta tgttcattcg cttgggtctg ctctacaata
     9541 aaaaacaata aaaaataaat agacgtattt tcaaaaaaaa aaaaaaaatg catatttata
     9601 ttagtaaaat aacgatttaa atcgtcgttt ttttgt
//
LOCUS       DAVKJU010000070         9403 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVKJU010000070 DAVKJU010000000
VERSION     DAVKJU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.070"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..593
                     /locus_tag="VW191_001756"
     CDS             <1..593
                     /locus_tag="VW191_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9624485.1"
                     /translation="KDQEYKRNGYYTIDLFSPIYSALSGEKGTPGDLMGRRIAFELLA
                     AKGYKEGMVPYISNQYEKDAKAVGSKINSYGKEVGLVTDELVLEKVFNGQYKTWTQFK
                     KDMYKEREKQFSKLNRVNFINPNNPLSRQRNVSVTDIGVLERMIVEAVRDDAQDDVAK
                     FYPETNSRVLKLKKAIYKAYLDQTNDFRSSIFENKK"
     gene            637..5886
                     /locus_tag="VW191_001757"
     CDS             637..5886
                     /locus_tag="VW191_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9624486.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDV
                     KKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHY
                     IEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLL
                     SDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIEN
                     VKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVE
                     RAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVV
                     GSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSY
                     QRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYK
                     VDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDI
                     DHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKD
                     RLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVK
                     SLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFK
                     PDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFT
                     NDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAH
                     MFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQ
                     LLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGA
                     MASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAY
                     AQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDA
                     LMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINN
                     IDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINIMTGIYGGNTSQGAPGAASFKHNT
                     FRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYF
                     KDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLK
                     SKVFKALLKNTDGFFNKLFKEDI"
     gene            6132..6983
                     /gene="ylqF"
                     /locus_tag="VW191_001758"
     CDS             6132..6983
                     /gene="ylqF"
                     /locus_tag="VW191_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEU9624487.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            6970..7749
                     /locus_tag="VW191_001759"
     CDS             6970..7749
                     /locus_tag="VW191_001759"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU9624488.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIVGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            7765..9315
                     /locus_tag="VW191_001760"
     CDS             7765..9315
                     /locus_tag="VW191_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU9624489.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGISKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSIEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         3175 a         1557 c         1931 g         2740 t
ORIGIN      
        1 acaaagacca agaatataag agaaatggtt attatacaat tgatttattt tcaccgattt
       61 attcagctct tagtggagag aaagggactc ctggtgattt aatgggacgc cgtatagcat
      121 tcgaattatt agctgcaaaa ggatataaag agggcatggt accttatatc tcaaatcaat
      181 atgaaaaaga tgcaaaagcg gtgggaagta agattaattc ttatggtaaa gaagtaggtt
      241 tagttacaga tgaattagta cttgaaaaag tatttaatgg tcaatataaa acttggacac
      301 aattcaagaa ggatatgtat aaggaaagag aaaaacaatt cagtaaacta aatagagtta
      361 actttattaa tcctaataat ccattgagtc gtcaacgaaa tgtatcggtt acggatatag
      421 gtgtactaga gagaatgatt gtcgaagcag tgagagatga tgcgcaagat gacgtagcaa
      481 aattttaccc agaaacaaat agtagagttc ttaaactgaa aaaagcaata tataaagctt
      541 atcttgacca aacaaatgat tttagaagtt caatttttga gaataaaaaa tagtgtttac
      601 tattaggaaa taaaaagtga atgtggagga ttagaattgt cattattaaa aaaagataaa
      661 ttttccattc ggaaaataaa gggtattgtt ggttcggtat tcttggggag tcttttattt
      721 gcaccgtcag ttattggtgc atcgacttat cattacttag attatagtag tttgacacaa
      781 actgaacgtg atcaactcaa acaaggtaga cctgatgaat ccaaagagtc atatgctttg
      841 gattatgaaa aagacgcact accaaacaca ggcagttctc aatctattat gactgcattg
      901 ggtttgttag ctatcgggag tcttattgtg attatcacaa aagataatag aaataaaaaa
      961 atagctacat ttttgattgt aggggcgaca ggtttagtta cactatcaac tgcttcagca
     1021 cttaatttga atgctaacat ccatgagtct ggacgtgatg gtgtgttgca aatttctggt
     1081 tacagatatg ttggttactt ggagcttgat gataaaactg tttcgtcggt ttctccagca
     1141 tcaactgttt cgccagttga gcaacccaaa gttgtaacag agaaagggga accggaggtt
     1201 caaccagcct tacccgaagc tgttgtgact gacaaaggcg aaccggaagt acaaccgaca
     1261 ttaccagaag ctgttgtgac tgacaaaggc gaaccagaag ttcatgagaa gcctgactat
     1321 acgcaaccaa taggtgcaaa ccttgttgag cctgaagttc atgagaaact agcatacact
     1381 gaaccagtag gtacaacagg agtggatgaa aatggaaact tgattgaacc acctgttaac
     1441 gacattccag agtatactga accaatatct acagtttcag aagttgcatc agaaagagaa
     1501 gagttgcctt ctttacatac cgatattcgt acagagacta ttcctaaaac cactatagaa
     1561 gaatctgatc ctagcaaatt tataggcgat gattctgtta aagaagttgg tgaggatggc
     1621 gaacgtcaaa ttgttactag ctatgaagag ttacatggca aaaaaattag tgaaccagtt
     1681 gaaacggtaa caattttgaa agaaatgaaa cctaaaattc ttgtaaaagg tacaaaagaa
     1741 aatccaaaag aaaaaacagt tcccgttttg accttgacca aagtaacaga agatgctatg
     1801 aatcgttctg ccaacttaaa ttatgagctt gataataaag ataatgctga aatatcaagt
     1861 attatagctg aaattaaaga tggtgataca gtagttaaaa aggttgattt atctaaagaa
     1921 aaattaactg acgctgtaca aaatttggat ttgtttaaag attataagat tgcaacaaca
     1981 atgatttatg atcgtggtca aggttctgaa acgtctaaac tggatgaaaa aactttgcgt
     2041 ttggagttga aaaaggtcga aattaaaaat atttcatcta ctaatttagt gaaggtaaat
     2101 gatgatggta ctgaaatacc tagtgatttc atgagcgaaa aaccttctga tgaagatgtg
     2161 aagaaaatgt acttaaaaat cactagtcgt gataataaag taactcgttt agcagttgat
     2221 aagattgagt tagtaactga aaaagaaaaa gaactttata aaattacagc aagcgctcaa
     2281 gatttaattc aacatgtcga tccatctaag actagaaatg aatacattca ttacattgaa
     2341 aaaccagttc caaaagtcaa taatgtatat tacaatttca atgaacttgt gagagatatg
     2401 caagaacatc caaatgacga gtttaaattg ggtgctgacc ttaatgccac aaatgtatca
     2461 gcatttggta aatcatatgt tacgaaagat ttcaaaggta agctcttgag tgatggtgat
     2521 aatcactata ccattcataa cttgtcacgt ccgttatttg gcaatgttat tggtggaacg
     2581 attaagaata ttaatcttgg taatgttgac ataaatatgc catgggcgaa ccaagttgca
     2641 gctgtagcga atattattaa aggtggaact accattgaaa atgttaaggt taaaggtaat
     2701 atagtaggta aagattgggt atctggattt attgataaga ttgataacca aggtacactc
     2761 agaaatgtgg cctttatcgg taacgtaact tctgtcggcg atggtggaca attcttgact
     2821 ggtatcgttg gtgagaactg gaaaggactc gtcgaacgcg cgtatgttaa cgcaaatctt
     2881 attggcaaga aagccaaggc tgcgggcatt gcttattgga cacaaaatga aggaaataac
     2941 aatactgttc gtcaggaagg agcaatcaaa aaatctattg ctaagggaac aattcaagta
     3001 actgaggcga ttgaaagtgg tggtgttgta ggaagcatga agcaccacgg ttctgtcgaa
     3061 gattcagttt ccatgatgaa ggtacctaat ggtgaaattt tctatggttc tagtgatatt
     3121 gattatgatg atggctactg gacaggagat aatgtaagac gaaattatgt tgtaataggt
     3181 gttagtgatg ggcattctag ctaccagcgt tcaaaagata aaaatcgtat cagacctatt
     3241 tctgaggaag aggcaaaatc taaaattgaa gctacaggaa ttaccgcaga taaatacgaa
     3301 atcaatgaac ctgtggtcaa tcgcttaaat cgtttaacca gaagagaaga tgaatataag
     3361 tcaacccaag actataaagt agatcgagat ctggcttatc gtaatattga gaaactgcaa
     3421 ccattttata acaaagaatg gattgtcaat caaggtaaca aacttgcaga agattcaaac
     3481 ttagctaaga aagaagtcct ttctgttacg ggtatgaagg atggtcaatt cgtgaccgat
     3541 ttatcagata ttgatcatgt gatgattcac tatgcagata agactaaaga aatcaaagct
     3601 gttcatcaaa aagagtctaa agtagcgcaa gttcgtgagt atagtatcga tggattagat
     3661 gacattgttt atacaccaaa catggttgat aaaaatcgtg atcaattgat taaagacatt
     3721 aaagataggt tagcaactgt tgaattaatt tctcccgaag tccgtgcttt gatggataaa
     3781 cgagatacat ctagagatcc taatgctaat tcggatgagc gtaagaatgg ttatatccga
     3841 gacctttatt tcgaagaaag tttttctgaa acgaaagcta atcttgataa attggtcaaa
     3901 tctttgattg aaaatgctga ccatcaatta aatagtgacg aagcagctat gaaagcactt
     3961 gttaagaagg tggatgaaaa taaagctaaa atcgtgatgg ctcttactta ccttaatcgt
     4021 tactatgata taaagtatgg ggatatgact atcaaaaact tgatgatgtt taaacctgat
     4081 ttctatggta agtcagttga ccttcttgac ttcttgattc gaattggatc aagtgagcga
     4141 aatatcaagg gtgacagaac tttggatgct tatcgtgata tgattggtgg aactattggt
     4201 aagtctgaat tacatggctt cttagattac aacatgcgtc tcttcactaa cgatactgat
     4261 ttaaatgatt ggtttatcca tgcggctaag aacgtataca ttgtggagcc taaaacaacg
     4321 aacccagatt ttgttaataa acgacatcgt gcatttgatg gtttgaataa tggtgttcat
     4381 aatcgtatga tcctacctct attaactctt aagaatgcac atatgttctt gatttcgact
     4441 tacaatacaa tggcttatag ctcttttgaa aaatatggta agtatacaga agctgaacga
     4501 gaagcattta aagataaaat taaagaagtt gctcacgcac aacaaactta tcttgacttc
     4561 tggtcgcgtt tagcattgcc aagcgtacgt gaccaattac tcaagagtca aaatagggtt
     4621 ccaacacctg tttgggataa ccaaaactat cataacgtcg aaggtgtaaa tcgtatgggt
     4681 tatgataaga ataataaacc aattgcacct attcgtgaat tatatggacc tacttggcgt
     4741 tatcatacta caaattggta catgggagct atggcatcta ttttccaaga tcccaataat
     4801 aatgaccaag tttatttcat gggaacaaat atgattagtc cgtttggtat ttcagccttt
     4861 actcacgaaa caacacacgt aaacgataga atgctttact tcggtgggca tagacaccgt
     4921 caaggtacag acgttgaagc ctatgcgcaa ggtatgttgc agacacctga taaatctggt
     4981 aacggtgaat atggtgctct tggtcttaac atggcttatc atcgtgaaaa tgacggtgac
     5041 caatggtata attatgatcc agataaattg aaaactcgtg aagatatcga ccgttatatg
     5101 agaaattata atgatgctct tatgatgtta gaccatcttg aggctgatgc tgttatccct
     5161 aaattacatg gtaatattag tcgatggttc aagaaaatgg atagacaata tcgcaaaaat
     5221 ggagagctac atcaatttga taaagttcgt gaattaactg aagatgagaa gaagaaaatt
     5281 gttatcaaca acattgatga tttagtcaac aataatttga tgacaaaaca tggcgctcct
     5341 agtgacagaa catataatcc agaagatttt gatagtgctt acgtaaatat taatataatg
     5401 accggtatct atggtggtaa tactagccaa ggagcacctg gagctgcatc attcaagcat
     5461 aatactttcc gtatgtgggg ctattttggt tatgaaaatg gattcattag ttatgcatct
     5521 agcaaatatc aaggcgaagc tgacaaaacc aataaaaaat tattgggtga tgatttcatt
     5581 atcaagaaag tatctaagga taaattcaat aatcttgaag agtggaagaa acaatacttc
     5641 aaagatgtta agtcaaaagc agaaaaagga tttactgcta ttgaaattga tggtcgtcaa
     5701 attacaaatt atgctcagtt gaaaacttta tttgctgaag ctgttcaaaa agatattgat
     5761 ggtatgtcag atccgaaaat taaagatcat ttcaaaaata cagtagactt gaaatccaaa
     5821 gtattcaaag cccttcttaa aaacacagac ggtttcttta ataaactgtt taaagaagac
     5881 atttaaatgt ttaaaatata aagaggacag ttttttgctc cctctgaaaa gtcatcattt
     5941 gatggctttt ttctatatag ggtaaaagat agggtaaaag gctatcatcg gacaaaataa
     6001 agaaggcatg atataatata aagtagattt ttatatcata aaacaagaac tgtttggaca
     6061 tcattcattt ttagattgag atctcttaga tctagattaa cagcttcaga gctacgaaag
     6121 gaaaaaactc aatggctact attcaatggt ttcctggtca catgtctaaa gcgcgtcgac
     6181 aggtgcagga aaatttaaaa tttgttgatt ttgtgacgat tttagtagat gcacgcttgc
     6241 ctctatctag tcaaaatcct atgttgacca agattgttgg tgataaacca aaactcttga
     6301 ttttaaacaa ggccgacttg gctgatccag caatgaccaa ggaatggtgt cagtattttg
     6361 aatcacaaga aatccagacg ctagctatca actccaaaga gcaagtgact gtaaaagttg
     6421 taacagatgc ggccaagaag ctcatggctg ataagattgc tcgccagaaa gaacgtggga
     6481 ttcagattga aaccttgcgt accatgatta tcgggattcc aaacgctggt aaatccactc
     6541 tgatgaaccg tttggctggt aaaaagattg ctgttgttgg aaacaagcca ggggtcacaa
     6601 aaggtcaaca atggcttaaa accaataaag atctggaaat cttggataca ccggggattc
     6661 tctggcctaa gtttgaggat gaaactgttg cacttaagtt ggcattgact ggagctatca
     6721 aggatcagtt gcttcctatg gatgaggtta ccatttttgg tatcaattat ttcaaagaac
     6781 attatccaga aaagctggct gaacgcttca aacaaatgaa aattgaagaa gaagcgcctg
     6841 tgattattat ggatatgacc cgcgccctcg gtttccgtga tgactatgac cgtttttaca
     6901 gtctcttcgt gaaggaagtc cgtgatggca aactcggtaa ctatacctta gatacattgg
     6961 aagacctcga tggcaacgat taaagaaatc aaagaactcc ttgtgacagt caaggagtta
     7021 gaaagcccta tttttttaga gcttgaaaag gataatcgct caggagttca aaaggaaatc
     7081 agcaagcgta aaagagccat tcaagctgaa ttagatgaaa atttgcgctt ggaatccatg
     7141 ctttcttatg aaaaagaact ttataagcaa ggattgacct taattgtagg tattgatgag
     7201 gttggtcgtg gtcctcttgc tggtcctgta gtcgctgcgg ccgtcatttt acctaaaaat
     7261 tgtaagatta aaggtctcaa cgacagcaag aaaattccta aaaagaaaca tctggagatt
     7321 ttccaagccg ttcaagacca agccttgtcg attggaattg gtatcataga taatcaggtc
     7381 atcgaccaag tcaacatcta tgaagcaacc aaactagcca tgcaagaagc aatctcccag
     7441 ctcagccctc aaccagagca ccttttgatt gatgccatga aactggactt gcccatttca
     7501 caaacctcca ttatcaaagg agatgccaac tccctctcta tcgcagcagc atctatagta
     7561 gccaaggtaa cacgtgatga attgatgaaa gaatacgatc agcagttccc tggctatgat
     7621 ttcgctacta atgcaggata tggcacagct aaacatctgg aaggcctcac aaaactagga
     7681 gttaccccaa ttcaccgaac cagctttgaa cccgttaaat cactggtttt aggtaaaaaa
     7741 gaaagttaat tgaaaggaaa taccatggag gaacagtcgg aaatagtccg ttctaagaaa
     7801 gaattcgcct ttgcatccag cactatacta tcccaagttg gtcgaggaat cattgtcggc
     7861 ctcatcgttg gaattatcgt cggatccttt cgtttcttaa ttgaaaaggg cttccacctg
     7921 atacaaggag tttatcaaga tcaagggtac ttagtgcgca atctttttgt actggttttg
     7981 ttttatatac tcatctgttg gctcagtgcc aaactaacac ggtcagaaaa agatattaaa
     8041 ggctcaggaa ttcctcaagt cgaagccgaa ctgaaaggcc tcatgtccct caactggtgg
     8101 ggcattcttt ggaaaaaata tgtgctaggt attcttgcta ttgccagtgg actcatgctg
     8161 ggtcgagagg gacccagtat tcaacttgga gcagttggtg gtaaaggaat ttccaagtgg
     8221 ctcaaatcca gtccagtaga ggaacgttcc ttgattgcca gtggagctgc agcaggttta
     8281 gccgcagcct ttaatgctcc tattgcagca cttctctttg ttgtagaaga agtctatcac
     8341 catttttcgc gctttttctg ggtctcaact ctagcagcca gcatcgtagc aaactttgtg
     8401 tctctactca tgttcggttt gacaccagta ttggatatgc cagataacat tcctcccatg
     8461 accctagatc agtattggat atatctcgtc atgggaattt tccttggatt ttcaggcttt
     8521 ctctatgaga aagctgtatt aaacgttgga agagtttatg acttgattgg tcaaaaaatc
     8581 catttggata gagcttatta tcccatcttg gcttttatcc ttatcatacc agtcggaatc
     8641 ttcttacctc aaatcattgg tggcggaaat cagcttgtcc tttctttaac tgaacaaaat
     8701 tttagtttcc aagttttatt agcttacttt ttgatccgct ttatttggag tatgattagc
     8761 tatggaagtg gactgccagg aggaattttc ctccccattt tagctcttgg ttctttgctt
     8821 ggtgccttaa ttggtgttat ctgtgtcaat cttggacttg tcagtcaaga gcaattccct
     8881 atatttgtca ttctaggaat gagtggctat tttggagcca tatcaaaagc tcccttaacc
     8941 gctatgatcc tcgtaactga gatggtagga gatattcgca acctcatgcc acttggtctt
     9001 gtcactcttg tttcttatat tatcatggat ttgctcaaag gtacgccagt ctatgaagcc
     9061 atgctggaaa aaatgcttcc agaagaagta tctatcgaag gagaagttac acttatcgaa
     9121 ataccagttt ctgataaaat tgctgggaaa caagttcatg aactcaactt accacacaac
     9181 gtcctcatca caactcaagt ccataatggc aagagccaaa cagttaacgg ctcaaccaga
     9241 atgtatctgg gtgatatgat tcacctggtt attccaaaaa gtgaaattgg aaaagtcaaa
     9301 gatttgttgt tgtagcatga gtatttacat aatttatgtt atgtaaatac tcggcttgat
     9361 ttatttagaa aaccgattct cagaaatgag atcggttatt ttt
//
LOCUS       DAVKJU010000071         9384 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVKJU010000071 DAVKJU010000000
VERSION     DAVKJU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.071"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            488..1531
                     /locus_tag="VW191_001761"
     CDS             488..1531
                     /locus_tag="VW191_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEU9624490.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1698..3785
                     /gene="topA"
                     /locus_tag="VW191_001762"
     CDS             1698..3785
                     /gene="topA"
                     /locus_tag="VW191_001762"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU9624491.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3891..4250
                     /locus_tag="VW191_001763"
     CDS             3891..4250
                     /locus_tag="VW191_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEU9624492.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4343..4975
                     /locus_tag="VW191_001764"
     CDS             4343..4975
                     /locus_tag="VW191_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEU9624493.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            5019..5675
                     /locus_tag="VW191_001765"
     CDS             5019..5675
                     /locus_tag="VW191_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU9624494.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5694..6326
                     /locus_tag="VW191_001766"
     CDS             5694..6326
                     /locus_tag="VW191_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEU9624495.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEECGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKK"
     gene            6354..6788
                     /locus_tag="VW191_001767"
     CDS             6354..6788
                     /locus_tag="VW191_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HEU9624496.1"
                     /translation="MVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEF
                     NATGQGSVEAIFNAIDKFFNQSVRLVSYTINAVTDGIDAQDRVLVTVENRDTETIFNA
                     AGLDFDVLKASAIAYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6800..7837
                     /gene="leuB"
                     /locus_tag="VW191_001768"
     CDS             6800..7837
                     /gene="leuB"
                     /locus_tag="VW191_001768"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEU9624497.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VEWIIRKAFEIARNRRKILTSIDKQNVLATSKLWRKVAEEVAQDFSDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIYGSA
                     PDIAGQGIANPISMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            7834..8103
                     /locus_tag="VW191_001769"
     CDS             7834..8103
                     /locus_tag="VW191_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEU9624498.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8107..8466
                     /gene="leuD"
                     /locus_tag="VW191_001770"
     CDS             8107..8466
                     /gene="leuD"
                     /locus_tag="VW191_001770"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEU9624499.1"
                     /translation="MVGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSKWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8685..8828
                     /locus_tag="VW191_001771"
     CDS             8685..8828
                     /locus_tag="VW191_001771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624500.1"
                     /translation="MTSDKAGLERKFAAKERKRNKPGVVLCGSMDELCALAQLNPEIE
                     AFY"
     gene            8847..9101
                     /locus_tag="VW191_001772"
     CDS             8847..9101
                     /locus_tag="VW191_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624501.1"
                     /translation="MLLGCILPWKPEAFEKLKAYGDGREELMTDVRGTSCFVIKFGKA
                     GEQLAAKLWEEGKMVYASYASMTKRLKLAMRARCNGVYGR"
     gene            9091..9252
                     /locus_tag="VW191_001773"
     CDS             9091..9252
                     /locus_tag="VW191_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624502.1"
                     /translation="MVDKDGKLIPEQGGARSTSPAPVVIRKGLDIDKIMMHLSDTFNS
                     WDYRQVEYY"
BASE COUNT         2899 a         1649 c         2088 g         2748 t
ORIGIN      
        1 caaggtgcac tgttatagat tcaatacact ataatttagt gtaattgaga aaggagaaat
       61 gattgtgatt gatgttggct aggttatgtt caatgattcc taccgtctca aatcttgtca
      121 gtaaggaaaa ataaattctt caaaagtaga gattacaagg cttgtttaag aaagaattca
      181 aagaccttga caaataaaaa taaaatggtt attataagaa atggtctaaa atagatgatg
      241 atacttttcg aaaatctttt tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc
      301 tacggttagc ttccgagttt gattttcatt tactagaaat gaaactgatg agagatatca
      361 gtagacattt gagtcaggat attatggaaa atgataaaaa gagctcgtga gattggcata
      421 tcagactact aaagtattga gtttgttagg attttagcga ctagttagct gggaaaggaa
      481 gatatttgtg acaaataata aactgtattc gttgatagaa tttagaaata aaatatatga
      541 agaattagaa ctttccagaa gtgatttagc gattttacta tgtgccatgc ttatcgcctc
      601 tatcggatta aatatggatt cgactcccgt gattattgga gccatgttaa tctctccttt
      661 gatgacacct attctgggag tggggctctc tctagctata tttgatttta aattgttaag
      721 aaaatctttt aaaatattag ctattcaaat tcttgccagt ctaatagctt caacacttta
      781 tttttatctt tctcccattt cgtatgctag ttcggagatt gttgctagaa cctctccgac
      841 tatttgggat gttctcattg cttttgtagg agggatagca ggtattattg gtgctaggaa
      901 aaaagagacc aataatattg ttcctggtgt tgctattgca accgccttga tgcctcctct
      961 ttgtacagta ggttatgcta ttgcttctgc taatctaaaa tttatcatag gctcctctta
     1021 cctattcctc atcaattgta gctttattgt cattgcgact tatataggtg ttaggttgat
     1081 gatggttaag aaacattatt ttaaagataa tgaagaagac tctaaaatgc gtaggatttt
     1141 gcttctagtt gctgttttgc tgatgattcc gagtttcatc tctgcaacga ctttagtgag
     1201 agaaacgttg aaaaaagagt cccttaagaa atttatatca gagcagtttc aggggtataa
     1261 tattttgaaa aaaacctatt ctaaaaagac tcatacccta aagctaacta tttcaggaaa
     1321 ttatttgaca gaagaagaac tcgatatgat ttcaagtaag agaggtgact atggtttaag
     1381 tgatgtttct gttcaagttt cacaattgtc tgattcagaa caacttagta aggaagagct
     1441 ggtggagtat ttcttccagt atatcaagga taaggaagca aaagaaaagg agaaagccaa
     1501 taaatttgat acagagtctg aggagcaata atttcttgag aatagctggt ttctctcgtg
     1561 agtcttctat gtatatcaaa ggaagactga ggttttaagt ataaaacttt tcttctatta
     1621 tagtagctaa gtcttgacag ggttcaaaaa atggtttaca ctttataaag tttattactt
     1681 tgaaaaggtg tgatactgtg gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa
     1741 atctagtcat cgtggagtcg cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa
     1801 actacaaggt tttagccagt gtcggacata tccgtgattt gaagaaatcc agtatgtccg
     1861 tcgatattga aaataattat gaaccgcaat atatcaatat ccgaggaaaa ggccctctta
     1921 tcaatgactt gaaaaaagaa gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg
     1981 accgtgaagg agaagcgatt tcttggcatt tggcccatat tctcaacttg gatgaaaatg
     2041 atgccaaccg tgtggtcttc aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag
     2101 aacctcgtaa gatcgatatg gacttggtcg atgcccaaca agctcgtcgg atcttggatc
     2161 gcttggtagg gtattcgatt tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag
     2221 caggtcgcgt tcagtccatt gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg
     2281 ccttccagcc agaagaatac tggacagttg atgctgtctt taaaaaggga accaaacaat
     2341 ttcatgcttc cttctatgga gtagatggta aaaagatgaa actgaccagc aatgacgaag
     2401 tcaaggaagt cttgtctcgt ctgactagta aagacttttc agtagatcag gtggataaga
     2461 aagagcgcaa gcgcaatgct cctttaccct ataccacttc atctatgcag atggatgctg
     2521 ccaataaaat caatttccgt actcgaaaaa ccatgatggt tgcccaacag ctctatgaag
     2581 gaattaatat cggttctggt gttcaaggtt tgattaccta tatgcgtacc gattcgactc
     2641 gtatcagtcc tgtagcgcaa aatgaggcgg caagcttcat tacggatcgt tttggtagta
     2701 agtattctaa gcacggtagc aaggtcaaaa acgcatcagg tgctcaggat gcccatgagg
     2761 ctattcgtcc gtcaagtgtc tttaatacac cagaaagcat cgctaagtat ctggacaagg
     2821 atcagctcaa gctatatacc cttatctgga atcgttttgt ggctagccag atgacagcgg
     2881 ccgtttttga taccatggct gttaaattgt ctcaaaaagg ggttcaattt gctgccaatg
     2941 gtagtcaggt taagtttgat ggttatcttg ccatttataa tgattctgac aagaataaga
     3001 tgttaccgga catggttgtt ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac
     3061 atttcaccca accgcctgcc cgttattctg aagcaacact gattaaaacc ttagaggaaa
     3121 atggggttgg acgtccatca acctacgcgc caaccattga aaccattcag aaacgttatt
     3181 atgttcgcct ggcagccaaa cgttttgaac cgacagagtt gggagaaatt gtcaataagc
     3241 tcatcgttga atatttccca gatatcgtaa acgtgacctt cacagctgaa atggaaggta
     3301 aactggatga tgtcgaagtt ggaaaagagc agtggcgacg ggtcattgat gccttttaca
     3361 aaccattctc taaagaagtt gccaaggctg aagaagaaat ggaaaaaatc cagattaagg
     3421 atgaaccagc tggatttgac tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc
     3481 gttttggtaa attctacgct tgtagcaatt tcccagattg ccgtcatacc caagcaatcg
     3541 tgaaagagat tggtgttgag tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa
     3601 ccaagcgtaa tcgcctattc tatggttgca atcgctatcc agaatgtgaa tttacctctt
     3661 gggacaagcc tgttggtcgt gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag
     3721 tccgtggtgg tggcaagcag gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta
     3781 aataagagga ggggcccgaa agtaaatttc aggctccttt tggttagaac ttgacaaaat
     3841 acatcatttt atgcgaaact agaagaagat tattttaact aggagaagtt atgcgtctta
     3901 tctatctgat aattggtttt ttatcactgg ccttggctat tgttggggtt gttttaccct
     3961 tgttgccgac aacgcctttc cttttgttgt ctattgcttg tttctccaga agttccaagc
     4021 gtttcgaaga ctggctttat cataccaagc tctatcaaac atatgtagct gattttcgcg
     4081 agactaagtc tattacgcgt gaacgaaaga aaaaaatcat cgtctctatc tacgtcttga
     4141 tgggaatttc tatttatttt gcacctcttt taccagtcaa aatcggtctg ggtgctttga
     4201 ccatctttat cacctattat ctcttcaagg tcattccaga caaagaatag ttaaaacagt
     4261 agttatttgc cttgataaaa ttgaaagcat attcataaca atatgatata ataaatttga
     4321 agtaatattc aaggagaatc aaatgattta cgaattttgt gctgaaaatg tgactttact
     4381 tgaaaaggcg atgcaggctg gagctcgtcg gattgaactc tgtgataatc tagcagttgg
     4441 tgggacaaca cccagctatg gagtgactaa ggcagcggtt gaactggcag ctaactacga
     4501 tacaaccatc atgaccatga ttcggccacg tggtggtgac tttgtctata atgacctaga
     4561 aattgctatc atgctagaag acattcgttt gactgctcag gctggaagtc aaggggttgt
     4621 atttggagct ttaactgctg ataaaaagtt ggataagcct aatctggaaa agttaattgc
     4681 tgcatcaaaa ggaatggaaa ttgtctttca catggccttt gatgaactaa gtgatgaaga
     4741 tcaaccggaa gctattgact ggctcagtca agccggtgtc actcgtatcc taactcgtgc
     4801 tggtgtgtct ggcgactcct tagaaaaacg ttttgttcac tatcacagaa ttttggagta
     4861 cgctaaaggt aaaattgaaa ttctaccagg tggggggatt gaccttgaaa accgtcaaac
     4921 ctttatcgac caggtggggg taacacaatt gcatggtact aaggttgttt tttaaaaaat
     4981 agaaaggaac tgctagcttt gggtagcagt tttcacttat gtttgaaatt tttaaatcct
     5041 atcaatttaa tcaagaaaag gctcatgatt atggttttat agaaaatagc gaagtctgga
     5101 catatagttg ccagattttg caaggtgact ttgtcatgac tgtgtccatc actgctgata
     5161 atgtgaactt tcaagtcttt gaccaagaga ctggtgacct ctatcctcac gtttatatgg
     5221 aaagcatgag gggaagtttt gtcggaaatg tccgtgaggc ttgtctggag attctttacc
     5281 agattcggaa ggcttgtttt gatgtgcaag attttatctg tcatcagact aagcgtatca
     5341 tgactcaagt tcaggaaaag tatggaaacc agttggagta tctgtgggaa aaatcgcctg
     5401 atacagctgt attgcgccat gaaggcaatc aaaagtggta tgccgtcttg atgaaaatct
     5461 cttggaataa gctggaaaag ggcagagaag gacaagtgga agcagtcaac ctcaagcatg
     5521 accaagtagc taatttgctt tcacaaaagg ggatttatcc agccttccat atgagcaagc
     5581 gctactggat tagtgtgtcc cttgatgata ctttatcaga tgaagaagta ctggaattga
     5641 tagaaaaaag ttggaactta acctctaaaa aatgaaatat tttaataatt ttcatgaact
     5701 ttcaattagc taaatattct ttactgaaga aattttcaga aaatatagga tttaccacac
     5761 cagaggaatg tggtgccatc ttcaaatacc tgattgagaa tgtcaagacg gatcgtcaga
     5821 tcatctattc gcctcactgt catgatgacc tcggaatggc agtggcaaat agccttgctg
     5881 ctgtcaagaa tggtgcagga cgtgttgaag ggactatcaa tggtattagg gagcgagctg
     5941 aaaatgctgc tttggaagaa attgcagtgg ctctcaatat tcgccaagat tactaccaag
     6001 tagaaaccag tattgtccta aatgagacca tcaatacgtc agaaatggtt tctcgcttct
     6061 ctggtattcc agttcctaaa aacaaagccg tcgttggtgg caataccttc tcccacgaat
     6121 ctggtattca ccaagatgga gtccttaaaa atcctctcac ttatgagatc atcacacctg
     6181 aattggttgg tgttaagatt ccgcttggaa aattatctgg tcgccatgct tttgttgaga
     6241 aactgagaga attggcccta gattttacag aagaggatat caaaccactc tttgctaagt
     6301 tcaaggcact ggccgataag aagtaagaaa tcacagatgc agatactcga gctttggtag
     6361 ctggaaccat ggttgaaaat ccagaaggct tccactttga tgatttacaa cttcaaactc
     6421 atgcagataa tgacattgaa gcgctcgtta gcctagccaa tatggatggt gagaaagtcg
     6481 aatttaatgc gacagggcaa ggttccgttg aagcaatctt taacgctatc gataagttct
     6541 ttaaccaatc tgttcgtttg gtgtcctaca ctatcaatgc ggtaacagat ggaatcgatg
     6601 cccaggatcg ggttttggtc actgttgaaa acagagatac agaaaccatc tttaatgcag
     6661 cagggcttga ttttgatgtg ttgaaggctt ctgctattgc ctatataaac gctaatacct
     6721 ttgttcaaaa agagaatgca ggtgagatgg gacgcagtgt ttcttaccac gatatgccta
     6781 gtgtgtaaag gagaaggcta tggcaaagaa aatagtagct ctagtaggag acggaattgg
     6841 cccagaaatc atggaggctg gtttagaagt tctggaggct ctagctgaaa aaacaggttt
     6901 tgactatgag attgacagac gaccgttcgg aggtgcagat attgatgcag caggacctcc
     6961 cttacctgat gaaaccctta aggcaagtag ggaagcagat gctatcctac tagcagctat
     7021 cggtagtcct cagtatgatg gagcagcggt tcgccctgaa caaggcctga tggctctccg
     7081 taaggaactc aatctttacg ctaatattcg tcctgtaaaa atctttgaca gtctcaagta
     7141 tttgtcacca ctcaaaccgg aacgaatttc tggtgtagac tttgtcgtgg tgcgtgaatt
     7201 gacaggcgag atttactttg gagatcatat tcttgaagag cgcaaagcgc gtgatatcaa
     7261 cgactatagc tatgaggaag tggagtggat tattcgcaaa gcctttgaaa ttgcaagaaa
     7321 tcgcagaaaa atccttacta gtatcgataa gcaaaatgtt ctagcgacct caaaactctg
     7381 gcggaaagta gctgaggaag tcgcacagga tttctcagat gtaaccttgg aacaccagct
     7441 ggtagactca gctgctatgc ttatgattac caatcctgct aagtttgatg ttattgtaac
     7501 ggagaatctt tttggagata ttttatctga tgaatcaagc gtcttatctg gtacacttgg
     7561 ggttatgcca tcagccagtc attctgaaaa tggaccaagt ctctatgaac ctatttacgg
     7621 ttcagcacct gatattgcag gtcaaggaat tgccaatcct atttccatga ttttatcagt
     7681 tgccatgatg ttgagagata gtttcggacg ttatgaggat gcagagcgta tcaaacgtgc
     7741 tgttgagaca agtctggcgg caggaatttt aacgagagat ataggaggtc aggcttcaac
     7801 aaaggaaatg acggaagcta ttattgcaag gttatgaagt tagacgaaaa aattactcta
     7861 gtccttttga tttggaatgt catcattttc ttgatttatg gcattgacaa attcaaggca
     7921 agaagaagag cttggcgcat ccaagagaaa atcttactca ttttagccct tacttgtggt
     7981 ggttttgggg cctggttagc tggaatcact tttcaccaca agactcgaaa atggtacttt
     8041 aaaacagttt ggtttcttgg gatggtgacc acactagtag ccttatattt tatttggagg
     8101 taatggatgg tagggtcttc gagggaacac gctgcttggg ctctagcgga ctatggtttt
     8161 aaggtcgtga ttgcaggatc tttcggtgac attcattaca ataatgaact caataatggc
     8221 atgttgccaa tcgttcagcc tagagaggtt agagagaaac tagcccagct aaaaccaacc
     8281 gaccaggtaa ctgtggactt ggaacaacaa aaaatcatct caccagttga agaattcacc
     8341 ttcgagatag atagcaagtg gaaacataaa ctcctaaata gtttggatga tatcggtatt
     8401 accttgcagt atgaagagtt gattgctgct tatgaaaaac aacgaccagc ctactggcag
     8461 gattagaaaa aatagaaaag gaaatatagt aaactgaaat aagatgtaaa caaatgaatt
     8521 ggtgcttaac atccatttcc agcaattttt tagaaactac agtggactat tctggattca
     8581 acacattata aaattatgac aaaacacatt cacaagaagg ctacgacatt ttaaaaggtg
     8641 agggcggatg tatcgtttgc cctactaaag ttggttacat tatcatgacc agtgataagg
     8701 caggacttga gcgtaagttc gcagccaaag aacgtaagcg taacaaacca ggtgttgttc
     8761 tctgcggtag catggatgaa ctttgcgctt tagcgcaact caacccagaa attgaagcat
     8821 tctactaaaa acattgggat gaagatattc ttcttggttg tatccttcct tggaaaccag
     8881 aagcctttga aaaactcaaa gcatacgggg atggccgtga agaacttatg actgatgtac
     8941 gtggtactag ctgttttgtt atcaagtttg gaaaagcagg tgaacaattg gctgccaagc
     9001 tttgggaaga aggtaaaatg gtctacgcct catatgcttc aatgacaaaa cgattgaaac
     9061 tcgctatgag agcaaggtgt aatggtgtct atggtcgata aggacggcaa actcatccca
     9121 gaacaaggag gagcacgttc aacttcacca gcaccagttg tgatccgtaa agggcttgac
     9181 attgataaaa tcatgatgca cctgtcagat acttttaact catgggacta ccgtcaggtt
     9241 gagtattatt aggatagaga agaagtctag tgttatgaga tattaaagct cctaacactg
     9301 ggcttttgtt tagaatttct tttctttttc tataggatat ggtattctat gtagaaaata
     9361 tatgttaata agtaatgcca atat
//
LOCUS       DAVKJU010000072         9267 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVKJU010000072 DAVKJU010000000
VERSION     DAVKJU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.072"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(91..450)
                     /locus_tag="VW191_001774"
     CDS             complement(91..450)
                     /locus_tag="VW191_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEU9624503.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPIIAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(749..1663)
                     /locus_tag="VW191_001775"
     CDS             complement(749..1663)
                     /locus_tag="VW191_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9624504.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LQDSPGTNNFSLPNVSPTYGQYYTNQSEVIIGKNDLVKVKNTLQYPYSTSAYVESEFR
                     GVKNGKDVTYRGSANFIKDNVLITAAHNIYKHEFGKDAEELYVTPARTPNSVPFGRIK
                     VKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLGTLGLPTSQKNLTGITVTITGY
                     PSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSTVYDASNRVVGVHTLGDGAN
                     QINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(1813..1908)
                     /locus_tag="VW191_001776"
                     /pseudo
     CDS             complement(1813..1908)
                     /locus_tag="VW191_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1985..2296)
                     /locus_tag="VW191_001777"
     CDS             complement(1985..2296)
                     /locus_tag="VW191_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002876249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9624505.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(2221..2463)
                     /locus_tag="VW191_001778"
     CDS             complement(2221..2463)
                     /locus_tag="VW191_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEU9624506.1"
                     /translation="MSLADLLEELEAAKDSKKARSMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(2464..3096)
                     /locus_tag="VW191_001779"
     CDS             complement(2464..3096)
                     /locus_tag="VW191_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9624507.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(3110..4105)
                     /locus_tag="VW191_001780"
     CDS             complement(3110..4105)
                     /locus_tag="VW191_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9624508.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4102..4800)
                     /locus_tag="VW191_001781"
     CDS             complement(4102..4800)
                     /locus_tag="VW191_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEU9624509.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KIVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4878..5888)
                     /locus_tag="VW191_001782"
     CDS             complement(4878..5888)
                     /locus_tag="VW191_001782"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEU9624510.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(5872..6879)
                     /locus_tag="VW191_001783"
     CDS             complement(5872..6879)
                     /locus_tag="VW191_001783"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEU9624511.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIVFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     VYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVVSKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6866..7819)
                     /gene="mvaD"
                     /locus_tag="VW191_001784"
     CDS             complement(6866..7819)
                     /gene="mvaD"
                     /locus_tag="VW191_001784"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEU9624512.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEVVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(7801..8679)
                     /gene="mvk"
                     /locus_tag="VW191_001785"
     CDS             complement(7801..8679)
                     /gene="mvk"
                     /locus_tag="VW191_001785"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEU9624513.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8801..9130)
                     /locus_tag="VW191_001786"
     CDS             complement(8801..9130)
                     /locus_tag="VW191_001786"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU9624514.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2759 a         2129 c         1617 g         2762 t
ORIGIN      
        1 ctaactaaaa cactcataaa gtatagacaa ttttagacta ctttggatat cttttggtta
       61 gtcattttcc aaaactaatt tatctaattt ttaagttata tttgacttca tcttctcagt
      121 aacatgagta taaacactta aaatctttgt ttcattagta taatctacac gatccattat
      181 cgctattata ggtacttcca attcagcaag caaacttata tgcgaatatc taaaaacatg
      241 agcagtcaat acaaaatcat ctttcttaat ttcacttttc ctaaacctgt ttttcaaaaa
      301 gtaattgaaa caagttatca ttaagggaga accattattt gtaaatatat aatcaccttc
      361 ttctaaaaga agatattctt ttaaaatttc taacactcta tcactaatac caattactct
      421 atatgaagct aaacttttcg gactatccaa aaacacttcg ttattggacg attacattat
      481 agtaaaacga aataagaaca ggacaaatcg atcaggacag tcaaatcaat ttctaacaat
      541 gttttagaag tagaggtgta ctattatagt ttcaatctac tatacaagac gaaattgaag
      601 aaatctttcc attataaaac gcatattatc aaggttttta aacacctaat aatatgcgtt
      661 ttttcctatt ttaaagactt ttatccacct attatacaaa gttaagtatc catcacagtt
      721 tacaattagc cttcattacc taacccattc accatccttc aagagagtaa cctttaagaa
      781 ccgaataaat aaatggcaaa tttcgttcat ttaatttaac tgcactgtta atttgattag
      841 ctccatctcc taaagtatgc actcctacta cgcggttact agcatcataa actgtagatc
      901 cactagaccc ctctaaagta tcaacttggt agtccaagaa catgccatca tcacttaaaa
      961 cttgtttttt atctgtatac atttgatgaa ttttaaaatt atatgatgga tagcctgtga
     1021 tagtcacagt tattcctgtc aaattttttt gactagtagg aagacccaaa gtccctaatt
     1081 ttgcaccaat gggctcttct agaattaata aagccaagtc atattccctt gcatccttag
     1141 aatttaaatt tctaaattcc ttcaaataac gaacttcttt tacttttatc cgaccaaacg
     1201 gcacactatt tggagtacga gcaggagtaa catatagttc ttctgcatct ttaccaaact
     1261 catgtttata aatattatga gcagcagtaa taaggacatt atcctttata aaattggcac
     1321 tccctcgata agttacatct ttaccatttt tcacaccacg aaattctgac tctacataag
     1381 ctgatgtaga ataaggatat tgtaaagtat tttttacctt aactaaatca tttttaccaa
     1441 taatgacttc tgactgattt gtataatatt gaccataagt cggtgaaaca ttgggaagtg
     1501 aaaaattatt agttcctgga ctatcttgta atgtctcact tacctcatat tcttcacctg
     1561 tactagaaat aacactcaaa gcagatactg tcgataactg gctagccaat aaagtactcg
     1621 caataattga aatacccaat tttttataaa cagttttctt cattattgta tcctcctaat
     1681 gtaattatag cgtactattc taaatttctt aatctactat agaatcaaga aatctaccac
     1741 cttctttaaa taccctccat tatcacataa acaggtaaac ttttcaatta atgactgcgc
     1801 ttttcaatca cgctagaggt acttacttgc ttctttgata ctaagttcag ccattctttc
     1861 cttgtttttc tcaataaagc atgttaccca agtgggattc gttctggtgg tcaatagcga
     1921 cacgtctcag tcagatatta tctgataggc tccattttat actcaatgaa aatcaaagag
     1981 caaactagga agctagccgc agattgctca aaacactgtt ttgaggttgc agatagagct
     2041 gacgtggttt gaagagattt tcgaagagta ttaagattat ttcttctagt tcagggtgtt
     2101 catacaccaa actccctact actcgatcta ggatatctac cgtgtcccac caggattttg
     2161 tcacgactaa ctgctctagc ttaggcaaat cggtttcctt tagataagac tgcattgctt
     2221 tcaaatagtt agcagccaca tattggtatt ttctaggctc cttttcccag caagtgtctg
     2281 caaaatccca atcgataatc tttgtttttt tcgcttctgg taaatatttt tatagagttt
     2341 atttctttca ggcaccgcaa tacctagaaa agaaaattga tggcgcatat aggcttccat
     2401 ggaccttgct tttttagagt cttttgctgc ttctagctcc tcaagtaaat ctgctaaact
     2461 catctaaaac tcctcatgcc cgaccaaatg gtgctgaaag gcatagacag ccgcctgggt
     2521 acgatcgcta acttcaagtt tggcaagaat gttggacacg tgggtcttga ccgtcttgag
     2581 agagataaag aggtcatctg cgatgcgctg attttcgtag cccttggcga tgagttggag
     2641 aacatctcgc tcacgcgcag tcaattcttc atgaagttcc atatgattgc ggtggtattc
     2701 aaccttcttg ctaacctctt gctcaatggc cagctcgcca gcagctacct tactgacggc
     2761 atgaagcaat tcatctgcac tagaagtctt gagcatatag cctttggcac cagcatctaa
     2821 gactggcatg attttttcat tgtccaaata agaggtcaca atcaaaatct tggcttcagg
     2881 ccattcttta aggattgcta aggtcgcgtc aatcccattc atctcaggca tgacaatatc
     2941 catgacaatg acatctggac gcagttccaa ggccaagtca atcccttgag acccgttgga
     3001 cgcctcaccc acaacttcta catcgtcttg gaggtcaaag tagcttttca aacccaaacg
     3061 gaccatttca tggtcatcta ctagtaaaat tttcatcttt actcctttat cattccttat
     3121 ctaacagggg aatacggata tcaaccgcca gcccttgctt gggagctgtc aagagttgaa
     3181 ccgtccctgc catatcttca acccgctcct tgatatttcg cagtccataa ctcaagtcgt
     3241 ctaagctccc taactggaaa ccaatcccat tgtccaccac cttcagttgc aattcaacat
     3301 ctgtctgata gaggtagaca tctaggcaag atgcctgggc atggcggagg gtattgctaa
     3361 tcaactcttg caggatacgg aagatatgct cctcgatttt cttaggcaat ttcgtcatat
     3421 tctgcttgag actaacccta agatcactct tgtcctcaag ctcttttaag agaatctgaa
     3481 tcccttctat caagctcttc tgctccagtt caactggtcg caaatgcaag agcaaaaccc
     3541 gcaaatcctt ctgggcagtt tctaaaatag ctgtgacact ctgcaactgg atctgcatct
     3601 tttctctatc caatttcaaa gcctgctgac tgatacccga taaaatcatg tgggccgcaa
     3661 acaactcctg actgactgta tcgtgcaaat cccgagcaat tcgcttccgt tctttctcga
     3721 tgatttcctc ttcctgagca aggctatgat tttcagcttt ttgaagagct tctgtcaaaa
     3781 ggttaagttt acctgataag gacttgaaac tggcatccaa atctggatct gcaacctgaa
     3841 ccacttcttg ccctgccaat aaacgcttga gattagcctg catttttctt agagaaagct
     3901 cttcgatccc tcgccaaaac agggctaaga gacaggttat ggacatgctg aaaaccaaca
     3961 ataaaaagac aaatttttct gttttttcga catcgtgcaa aaagatagac cagtcaaaat
     4021 caagtatttc cagcaagctg tgggagaaaa aaaagacaaa caggaaggag gtgagagcaa
     4081 taatgacata ggcttgtttt ttcatcctct aaccacctcc acatcaccaa tcatagtggt
     4141 caagaaaatc ttgacactct tgttactctt gagatagtct tttgtttctt gatgatagtg
     4201 ttcattgcgg agggctcgct tgggctggtt gaaaaaagtc aaatccccat agagacagtt
     4261 aacgctgaga ctgacttcca catctacagg tacgatgatt ttggtcgttc ctactatctt
     4321 tctgaggata atgacattgt catgattggt taagatgacc ctctccagat gaatagtgtc
     4381 cttgcccatg aagcgaaaga gattgatatc atcgaattgg caagtctggt agcttgaaaa
     4441 atgatgaaga tttccaaacc aacgattttt ctccttctta accgtcacga cctcttcaaa
     4501 aaccaaattg gtctgctctt tttcctggtt catcatcggg taaagaagaa agaggctata
     4561 gataaccgca acaaaaatag ctagaatcac aaaaggattg agcataacga tgaaaaagaa
     4621 gagaatggtt gccgctacta aaagaagatt atttccctct ttaccagtgt agtagcgaat
     4681 caaaagcaaa aagaggaata gtatcagcag aaaacgcgaa aaatgctctg ataccatcaa
     4741 aatcagagct cctgtcagaa gacaggcttc gataaataaa aagattttaa attttctcat
     4801 aggttcatcc tctcccttct attttatcac aatttaaaaa agtcacctca gtctgaggat
     4861 ggaaaaaaag gcgctggtta cgcctttttc atctgatcat ttgcttcttt taattttcca
     4921 taaagaagat agtctacttt ttgtagatct gctatggtgg cacagttaag ggaacacata
     4981 atcaagcgta gatctgcttt ccagccttgg acaatgccaa tcacttcttc aactgtgtag
     5041 gtttcaacca attccagaac ggttcgtgac aatcccacag ccttagcacc aaaaaccaag
     5101 cacttaatca tatccagcgg attccgaacc cctccactaa ccaagagttc gaccttatct
     5161 ttccattctt gggcattgag aagggcctgc atggtagact gaccccattg attgaggtaa
     5221 tcacgctggc tactacgacg gttttcgata taggcaaagc tggtgccacc acgacccgat
     5281 aggtccactg tacgaacacc gaattcatag gctctttcga ttgtcttggc atccattcca
     5341 aagcccactt ccttgaggac aataggaacg ggaatttgct tgctataatc tgctagatgc
     5401 gattgccagc ttctaaactt cctttctccc tcgggcatga gtaattcctg catgacattg
     5461 acatgcactt gcaatagaac aggattcatc tcttctacag tctgaagtcc taactcgaca
     5521 ggcttgtcca atccaatatt ggttccaagg aggagattgg gatgactaga cttgacagaa
     5581 aaagaatcat ccgttggatt tttgagggct gcgctataag aacccgttac aaataaaata
     5641 ccacaggatt ccgccacttg agccagcttt tgattgattt ctcttccctt attacttcca
     5701 ccagtcatgg cattgatata aaaaggaaag tcccactttc gaccagcaaa ctctgtcgaa
     5761 agatcgattt catccagatt gtaaagaggc aaggaagaat gaatcagctc cacctcatca
     5821 aagctattat aggaactttt ctgctcaagg gcatagagga tatgctcgtc cttacgattt
     5881 gtcgtcatgt cctatccttt cttgatataa gagctcaatc cccagatcgg cccaacgatt
     5941 ttttaaggtt ttggttgatt gcgcatcaaa actcagggcg atgccacagt caccaccacc
     6001 agcaccacta ctcttggcaa cggcctgcaa atcttgactg gcttctttca actgtctaag
     6061 caaaggcgtg taaatatctg tactcaagcc ttctaaaagc ttgctgacta cttctacttg
     6121 ctcgataact ttttcggctt tcccctgttc caaggcttcg accaaagaaa ccaccgtttc
     6181 ttttgaggaa cttaaaaaat tttgattgat attttgcttg atttgctgaa ccatgtgact
     6241 cgatacagcc acttccttgg tccatcccac taagaaatca cattctaaag ttggtttcac
     6301 ttgtgaaatt gaaaagcccc aatcacgctc cagaactgtc gccaagtttt cttcttctaa
     6361 ccaagcagcc accttctggc gatcaaatga ctggtagaca accaaatcct ctgccacaat
     6421 acaggcaagg tctcccatgg aaccattgtc tcctcgcttg agcaagacag cgctagtcag
     6481 cttgaacaag agctcctgat caacagaaac atcatacaga gccagtaaag ccttgacaac
     6541 caagacaacg acgctgccac tagaacctag accaaacttt ttcccttctc gttccatttt
     6601 gccacagatt tctagagaaa aaggtcttaa attctgacca cgaacagcga ggaagtctcc
     6661 catcaaagca atcgtttctt gaatcaagct atagtcagga ttgggcgtta agtccactgc
     6721 gaaatcaaac atatctgaat agatacggta gctgtcagaa aaaacaatct cagccctcat
     6781 atagatggga atatccttta tcaaagctaa ctgccctggc tctaaaatag catattcacc
     6841 tgcccaatag agttttccgc aagttttaac agcaatcatc ttgactcaaa tcctttgttt
     6901 ttgacacaat caagcgataa cgatgaccga aaatttctga taaatgctcc aagtctttct
     6961 cctgacagaa gaccttaaca ttgggaccag catccatggt aaagtagcag gcctctcctt
     7021 tctcacgaag ctggcgaaca aagtccatag cctcataaga ggcatccgtc agataagaaa
     7081 aggctggact agcagtcttt gtcgtagcat gcatagccag ggcatttttc tccgttaatt
     7141 ctccaatctt ggcaaaatca ttttccttga gataaatcag catatcctga tagtccttct
     7201 cagactgacg aacccagtcg tcgaaagtcg tcgaggtttc cacacaaagt ttcatcccgt
     7261 cacggctaga gattggtttt ttcttgtcct ctagcaccaa cataatcata gctagtttca
     7321 agtctgtctc tacagggtaa atttctccac tatccttatc ccaggctcct agtggtccat
     7381 aaaaactccg agaagaagag cctgaggcaa atttggcttc ctgtgccaac tgacttctat
     7441 ccaatccaag tttgaaataa gcattacaag ccttgaccag ggcggacaaa ccactagaac
     7501 ttgaggacag gcccgctgcc gtaggcatat tgttttgagt atcgatacgg acaaagccct
     7561 caccagctgg acgataacgg tcaataatct tactcatctt ggcatgctcg acctcatttt
     7621 gtagctgacc attgatgtaa aattcgtcag ctgttacatt ggctggtaaa ggcgacaagg
     7681 tcgtctctgt atacatattt tccaaagtta gagaaatact gctagtagca ggcaccacct
     7741 ctttttcttt tttctttccc caatatttga taatagcaat atttgcgtag gaacgtactg
     7801 ttacaggctc tctatccatg tctgaacagc tcctttctct tctaatcttt ctgctagttc
     7861 ttgtgcgtgt gtcaaattgg ttaccaaggc tatgatacaa cctcctagcc caccaccgct
     7921 catcttggca cccagagcac catggctaag agtcgtttca accaaaaagt ctgcctcagg
     7981 gctactgact ccaatttctt ttaaatgtaa atgcgcttga ctgaggattt gtcccagtcc
     8041 ttcagcatct ttttgtgaaa tcgcaacttc tgcttgctgg gttaattctc ccaaggcatg
     8101 caaaaacggt agggcatcct tgcccttatt ttgaaccact tggatggctt cacgagtatg
     8161 accataaaca cccgtatcgg caatcaccaa ataggcggat aaatccatct caagttctgt
     8221 aaatcctacg ttcttgataa agcgaatagg ttggtcactg agacaggtct tagcatccaa
     8281 accactagga ttcatatggg caatcatttc agctcgattg accaagattt ctagtacatc
     8341 atgaggcaga tcagcctgat agtagtcaaa taccgcacga atggccgcta tgctgatagc
     8401 cgctgacgaa cccatccccc gtttctcagg gatagccgag tcaatctcac aacgaatgca
     8461 ggcttctgtg atatccaaat actccagtga ggcataaacc gccatggaca aggtatcctc
     8521 ctcataaagg cgccaaggac tctctgcagg aactacctta caggtcacct ccacctccaa
     8581 aagaggcagg gaaatggcag gataaccgta aacgaccgca tgttccccta ttaaaattat
     8641 cttactatgt gcctgaccga caccaacttt ttttgtcatt ttttcctttt actagacgaa
     8701 aaaacgtctt atttttcata caagtattaa ttctttccta tctattttat tatattttca
     8761 caaaaaaagc gattgtttcc attcacaatc gcttctttca ttattgaacc cattcgccat
     8821 tatagttgac agaatagcca tctacggtcg tattcactgc caaggcacct gagctataag
     8881 cgtagtacca tctgccattg acctggaacc aacctgtcgt catagaacga cgaaagaaac
     8941 tccataccat taagtaaaga ggaaagtcgt gagggagcat gcgccattga caacctgttt
     9001 tagtgacgta caaagtctca ttaacaagta ctcgtttcgg ccatttatag gtgcggtgtt
     9061 tggagaaata gggttcaatc ttcgcccatt cttgatcatt taaatcagta tcatatgctt
     9121 tgcgtgtcat aactctagtt taacattttt ttgtgaatag aggttctaaa taatcgacca
     9181 cgaaaatttc ttaagtggaa aacgccttat gaagtatgct acgggaaagt tatgcactta
     9241 atttgacaat tcaagatgta aaaatat
//
LOCUS       DAVKJU010000073         9059 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVKJU010000073 DAVKJU010000000
VERSION     DAVKJU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.073"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(380..1228)
                     /gene="dprA"
                     /locus_tag="VW191_001787"
     CDS             complement(380..1228)
                     /gene="dprA"
                     /locus_tag="VW191_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEU9624515.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1324..2013)
                     /locus_tag="VW191_001788"
     CDS             complement(1324..2013)
                     /locus_tag="VW191_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU9624516.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFCNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2025..2903)
                     /locus_tag="VW191_001789"
     CDS             complement(2025..2903)
                     /locus_tag="VW191_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU9624517.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2893..3762)
                     /gene="licA"
                     /locus_tag="VW191_001790"
     CDS             complement(2893..3762)
                     /gene="licA"
                     /locus_tag="VW191_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEU9624518.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKGEQGADFGYYGVNRYQRA
                     VKGLASYGGTDEK"
     gene            complement(3779..4801)
                     /locus_tag="VW191_001791"
     CDS             complement(3779..4801)
                     /locus_tag="VW191_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU9624519.1"
                     /translation="MINQIYQLTKPKFINVKYQEKAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLL
                     LVGSSRSGRIDFENAIQMMKVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4806..5513)
                     /locus_tag="VW191_001792"
     CDS             complement(4806..5513)
                     /locus_tag="VW191_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU9624520.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVLHAEDLVDKYLPLHKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIKLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RVHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5868..7355
                     /gene="tacF"
                     /locus_tag="VW191_001793"
     CDS             5868..7355
                     /gene="tacF"
                     /locus_tag="VW191_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEU9624521.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDSKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7365..8168
                     /locus_tag="VW191_001794"
     CDS             7365..8168
                     /locus_tag="VW191_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9624522.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYVSGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8170..8979
                     /locus_tag="VW191_001795"
     CDS             8170..8979
                     /locus_tag="VW191_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEU9624523.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2834 a         1826 c         1533 g         2866 t
ORIGIN      
        1 aaattaattt gactttcctg atagagttgt tcacatctta tttcaattca ctatactttc
       61 ccttatactc aatgaaaatc aaagcgcaaa ctaggaagct agccacacag gttgctcaaa
      121 gcactgcttt gaggttgtag ataagactga cgaagtcagc tcaaaatact gttttgaggt
      181 tgcagatgga agctgacgta gtttgaagag attttcgaag agtattatat gagaaaaatc
      241 ctctactcta ctaatccact tagaaaaaac gcatattatc aagtgttaaa aacttgctaa
      301 tatgcgtttt attatggaaa tgttgggttc attttctctt aaaattgatt ttttcttaga
      361 attctagctt aggtcatttt taaaattcaa attccgcaag aacatcttgc ccactggtga
      421 ccaattttgc tccttcttga atcaaatgat ggcaaccgtc tgatagtcca tctaaaatgc
      481 taccaggaat agcaaagaca tcgcgtcctt cttccattgc tcgctcacac gtaatgagac
      541 tacctgaacg catcttagcc tctgctacaa tcacaccacg acaaagtcca gcaatgatgc
      601 gattacgggc aggaaaatga aatttcagag gttgttcgcc aggtccatat tcacttagaa
      661 ccagatggtc attgccgatg tagtcttgca agcgtttatt ggctttagga taaaacacat
      721 ccagtcctgt tccaatcact gcaatggttt ttccgccatt ctgaagagct gccatatgag
      781 ctgctgtgtc aatgcccttg gccagaccac tgacaataac cagttcattt tccaagcctt
      841 gaatgacttt ttcaactgac ttagctccct gtttgctaca agcacgactg cccacgaccg
      901 ctaccttcgg gaatttcagg aggtcaagat ttcccttgta aaataaaagt acaggcgcat
      961 catatatttc actcaaatcc caaggataac agtcatctaa aatagagaaa gatggaaatt
     1021 tttgaaactc tttcgacaaa tgcgcatcgt ctatctgaaa ataacgttcc ataaaaacgg
     1081 ctggattacg gcaacctgat atatctgcaa tatcacccaa caaaagctcc tgatcaacat
     1141 tttcaccgta ttctagcact ttcaaaatct gttgattggt caaacctgat ttttttaact
     1201 tatagatttc atagtttgtg attttcataa ataactccat ttctttttct actcatctat
     1261 ctattcgtaa aaattcgtaa aaaatcaaaa aacatccgac tattttgtca gatgttggaa
     1321 tctttaattt tcgtttttaa gaatttcttc taatttacga tagtcttgga cactatcgat
     1381 ctcataaatg ctattgcctt ctaattcttc aacatagaca tctagctctt tgatattatc
     1441 cttaaccata ttgtcccaat agagatcaac aaattcacca cttgcataag ccttgtcgat
     1501 aaagctgaca atcttttctg cagttggagc atcccagaag gatacaccac taaggatgcg
     1561 acctgccttg ctatcaacaa taatgtcttg aaccttgtag tcatctccat agaccaagaa
     1621 ccattcgttg gtacaatctt cacgataaac actaaaataa gtcgaacgtg tcaaatcatt
     1681 gcagaacata tttttaaaga gataattgtc agcatcaata acatagctgt tggccaattc
     1741 ttcttttaca agatagagag agtaaaagtt attgtagtca gcgtatttat cattgaaaac
     1801 gaggcgaaca ccgtattttt cttttaagta atcgaattgt tctttaagat aaccaacaat
     1861 gatgatgatg tcattgattc ctttttcttt gagaaactca atttggtatt caatcaaagg
     1921 tttttgatta acctgaacca aggctttagg ggtattttca gtcataggac gcaagcgagt
     1981 tcccaatcca gctgctaaga taatggcttt cacacgaatc tcctttattc tttaataata
     2041 atataaactc cagcaatgac gacaagtgac gtcataatgg tcagcatatc tagcggtgca
     2101 cccaaaaaaa caactgcaaa caagaccgtc catactacat agctcacgtt caagcctgta
     2161 gccttggctg gttgcaagcg attgatagcg atataataag ccaagtagga aatcatatca
     2221 aaggctgcaa aaacaatcat gagacctagc aattgtccat tggctactgc agtaaatgac
     2281 tgatgagaga agagcacaat cacaagatag gacaagaacg aagtcacttg acggattaag
     2341 agggcttcga tttcactcag ttcactttcc atagcaaagg agctaagaac actctcactt
     2401 ccccatgcaa tagcacaaac caaagcacag agaatcccaa tgtagaagga attaacctgt
     2461 tcaaccttat aggtctgggc aataatccct ccaataatca agacaatccc aaatacagta
     2521 tttttcgaaa tcttgtgctt caaaaagaag aaagccaata gaactgaaat cgcagggtaa
     2581 atagccgata cagatgaagc taaagaactt ccgatatact taactgcata aagattggcc
     2641 tgcataccga tagggcctgc tagcaaggct ccgatgataa cactgacatt gcgaatattt
     2701 aagaaaattg agaggcgaac tttcccttct tttaccaaga gaaaagctag taagataaag
     2761 atgctcaaaa aatcatgagc tgcagccacc acaaagggtg acaaatctgt aaaaatcgaa
     2821 aagatataag cactaaccgt tagacccaag ccccagaaaa tacctgagag aaggccaaaa
     2881 ggaactccgt ttttactttt catctgttcc tccataagaa gccaaacctt taacagctct
     2941 ttggtaacga ttcacaccat agtaaccaaa atctgcacct tgctctccct tatagacagt
     3001 ccatagactc caaatagtat cttgtaaaat tttataaata gcaatctttt catgagaaac
     3061 cggtgtttgg tcactctcat agtgagataa gaaagtttct tcctcttggg aagtgaattc
     3121 agactctaaa aagagggcag ccaaatccca cattggatca ttcattgatg aatattccca
     3181 gtcaatcaaa taaagtcgtc cttgaggtga ttcgataaag ttttcaggca ccaaatcgat
     3241 atgacaagat tttctgtcaa cacctaagtc agccagtctt ttctctaagg agaagactgc
     3301 atttctaaca gattcatagt tggcataagg aatttgttct tcaatcaagg attcgtattt
     3361 tttgatttct tcaaaaggag caaattctcc tcttaattcc ttagcagatg tatgaatagt
     3421 ttgtaatatt ggagcaattt tgtcgaactt ggtcttgatt gacgttgaat caagcgtaat
     3481 cgcagattcg atatactcat ttactttgat gccagcttca atatcaaaaa gataattttt
     3541 tacatctaag cctaaatcct ttagtagttc aagattgtac ttttcatctt gtcgattgat
     3601 aagcttttct gtccctttac caaagaattt aacaatgtat tgcttatttg ttgttttggc
     3661 caaatagttt tgattggtca ttccacccag ttgttcaaca ctgaggactt cttcttcttg
     3721 actaagtaag gaagaaattt tttctttaat gattttctcc acaattaacc tccaggactt
     3781 atacttccca cttaaacact gttttaaagg ctgtatttaa atcggttgca aagacacggt
     3841 gaatatcttt aatttctctt acaggttctt ctagataaag gatattttta agacgattgg
     3901 caaatttctt gactttcatc atttggatag cattttcaaa atcaatgcga ccagaacgag
     3961 atgacccaac caagagcaag cccttttcta aggcatcgcg agtattgaga ttgactttat
     4021 attcgctaac tcccatcatg agaattgttc cctgaggacg aatgtagcga atcaagtcat
     4081 taatagctgg tccagtacca tcaccaccac aacattcaaa agcatggtca aaggccaact
     4141 cttcaggaat attatccgta atatagcatt ctttggcaaa tgagaagagt tccaactttt
     4201 cccaatgacg accaataacc acaatctctg cttctggcaa agtataattg ataatattgg
     4261 caaccacaaa agctaaactt ccatctccaa taacggcgat ccgctcccgc ttgctatgag
     4321 caagagtcaa tagacgattc atagcgtgca tgcccacact gacaaactct gtaatggctg
     4381 caaccgtatc ttcaatagca tcataagcca ccacacgatc tttagggaga gaaacaaact
     4441 ctctcataaa gccatcaaat ccactagaca agaaatgggt ccctgtcatg tagttttcat
     4501 agaattcttc atcactctgc ataggagact gattgggaat catgacaact ttttgaccaa
     4561 cctcgtaggt tcctgtcggg tcagaaatga cggttccaca tgactcgtga atcattgcca
     4621 ttggaagctt tttattcaaa atcttgggat cacgttttcc ctggtagtaa cgctgatccg
     4681 catgacagac agccatgtag ttgggacgga taaggatatg attctcttgg tcaatagcct
     4741 tttcctgata tttgacattg ataaacttag gtttagttag ttgataaatt tgattaatca
     4801 ttttactagt ctttctcaat catacttttt gcaatcttca aatctgttac ggttgtaatc
     4861 ttcagatttg agtattcgcc tttggccaag gctacatctt ttcctttgat cacaaagatt
     4921 ttacatgcat ctgtcaagat ttccttctct tcatcagaaa gagatccata aaggtccatg
     4981 aagtccttgc aacggaatgt ttgaggtgtt tgtccctgat agaggtgaac acgatttgga
     5041 atacctgtaa tgaattgacc attggtactt tcaacgatag tatccactgc ttctactact
     5101 gtatccactg cgtcatgatt ttgagcaagt ttgatactgt cttgaatcat gcgaagcgta
     5161 ataaatggac gaacagaatc gtgggtaaca acgatatcct ctggagtaag cggacgataa
     5221 gcatcaatgg cttcaatgat gttctcaata cttgtattgc ggtcagcacc accctttgta
     5281 atgataatac gttccttatg aagaggaaga tatttatcta caagatcttc tgcatgtaaa
     5341 acccagtctc catgaacccc aactacaatt ttttcaatac ttggttccaa gacaaatttt
     5401 tcaattgtat gaatcaaaat aggtcgatca cctagctcta aaaattgttt tggcaagtta
     5461 ctgattccca tgcgtgtgcc agttccaccg gcaagaattc ctgcataaat catctatttt
     5521 ctcctttgtc ttactaaaaa acttattctc tctattatac cacaatctag tcctatatac
     5581 cacaatctag tcctatcatg aaagaaatac tggtcctccc cttctagaat aggagaatta
     5641 tgcagttcta ttattcaaag aaacaagcct atttctagta atgactagga attctgtagc
     5701 aattactaat aaatgactgc gaaattttag agtccttcag aataatatac tttccttaaa
     5761 gaaaacttaa aatttaagat ttgaaacttc aaagcgattg tttgacttgc tgcccttcat
     5821 ttcttattag ctaactttat gatataatga aaaacgaggt aaattgaatg aaaagtataa
     5881 aattaaatgc tctatcttac atgggaattc gtgtcttgaa tattattttt cccatcctaa
     5941 ctggaaccta tgtcgcgcgt gtcttggacc gaactgacta tggttacttc aactcagtcg
     6001 acactatttt gtcatttttc ttgccctttg caacttatgg tgtctatagc tacggtttaa
     6061 gggctatcag taatgtcaag gatagcaaaa aagatcttaa cagaaccttt tctagtcttt
     6121 tttatttgtg catcgcttgt acgattttga ccactgctgt ctatatccta gcctatcctc
     6181 tcttctttac tgataatcca atcgtcaaaa aggtctacct tgttatgggg attcaactca
     6241 ttgcccagat tttttcaatc gaatgggtca atgaagctct ggaaaattac agttttctct
     6301 tttacaaaac tgccttcatc cgtatcctga tgctggtctc tattttctta tttgttaaaa
     6361 atgaacacga tattgttgtc tatacacttg tgatgagttt atcgacgctg attaactacc
     6421 tgattagtta tttttggatt aaaagagaca tcaaacttgt taagattcac ctaagtgatt
     6481 ttaaaccact ctttctccct ctgacagcca tgttagtctt tgccaatgcc aatatgctct
     6541 tcactttttt agatcgcctc ttcctcgtta aaacagggat tgatgtcaac gttagttact
     6601 ataccatagc tcagcgaatt gtgaccgtta tagctggggt tgtaacaggt gcaattggag
     6661 tgagtgtgcc tcgtctcagt tactatctgg ggaaaggaga caaagaagcc tatgtttctc
     6721 tggttaatag aggtagtcga atctttaact tctttatcat tccactgagt tttggactca
     6781 tggttttagg accaaatgcc atcctacttt acggtagtga aaaatatatc ggaggcggca
     6841 tcttgacctc tctcttcgct tttcgtacga ttatcctggc cttagatacc attcttggtt
     6901 cccaaattct ctttaccaat ggctatgaaa aacgtatcac agtttataca gtctttgctg
     6961 ggctactcaa tttgggcttg aatagtctcc tctttttcaa ccatatcgtg gctcccgaat
     7021 actacttact gacaactatg ttatcagagg cttctctact tgttttctat atcattttca
     7081 tccatagaaa acaactcatc cacttgggac atatctttag ctatactgtt cgatactctc
     7141 tcttttcact ttcctttgta gcaatttatt tcctgattaa tttcgtgtat cctgtggata
     7201 tggtcattaa tttgccattt ttgattaata ctggtttgat tgtcttgcta tcagctattt
     7261 cttatattag tctacttgtc ttcacaaaag atagcatttt ctatgaattt ttaaaccatg
     7321 tcctagcctt aaaaaataaa ttcaaaaaat cataggagtt taaaatgaaa caactaacca
     7381 ttgaagatgc caaacaaatt gaattagaaa ttttggatta tattgatact ctctgtaaaa
     7441 agcacaatat caactatatt attaactacg gtactctgat tggggcggtt cgacatgagg
     7501 gctttatccc ttgggacgac gatattgatc tgtccatgcc tagagaagac taccaacgat
     7561 ttattaacat ttttcaaaag gaaaaaagca agtataagct cctatcctta gaaactgata
     7621 agaactactt taacaacttt atcaagataa ccgacagtac gactaaaatt attgatactc
     7681 gaaatacaaa aacctatgtg tctggtatct ttatcgatat tttccctata gatcgctttg
     7741 atgatcctaa ggtcattgat acttgttata aactggaaag cttcaaactg ctgtctttca
     7801 gtaaacataa aaatattgtc tataaggata gccttttaaa agattggata cgaacagcct
     7861 tttggttgct ccttcgaccg gtttctcctc gttattttgc aaataaaatc gagaaagaaa
     7921 ttcaaaaata tagtcgtgaa aatgggcagt atatggcttt tattccttca aaatttaagg
     7981 aaaaggaagt cttcccaagt ggtacctttg ataaaacaat cgatttaacc tttgagaatt
     8041 taagccttcc tgcacctgaa aaatttgata ctattttgac acaattttat ggagattata
     8101 tgaccctacc accagaagaa aaacgcttct acagtcatga atttcacgct tataaattgg
     8161 aggattagga tgcaatattt agaaaaaaaa gaaattaaag aaattcaact agccctgctg
     8221 gactatattg atgagacttg taagaaacat gatattcctt attttctcag ttatggaacc
     8281 atgcttggag ccatccgcca caaaggtatg attccttggg atgatgatat tgatatttcc
     8341 ctttatcgtg aggattatga gcgtttactg aagattattg aagaagaaaa tcaccctcgc
     8401 tacaaggttc tttcctacga tacatcttct tggtacttcc ataatttcgc atcgattttg
     8461 gacacttcta ctgttataga agaccatgtt aagtacaagc gtcatgatac cagccttttc
     8521 atcgatgtct tcccaattga tcgatttaca gacttgagca ttgtcgacaa gagctataag
     8581 tatgtggctc ttcgtcaact agcttatatc aaaaaatcac gagcagttca cggtgatagc
     8641 aaactaaaag attttcttag attatgtagc tggtacgctc tccgatttgt caatcctcgc
     8701 tacttttaca agaaaattga tcaactagtc aaaaatgctg taaccaacac tcctcaatat
     8761 gaaggaggag ttgggatcgg taaggaaggg atgaaagaaa tcttcccagt tgataccttt
     8821 aaagaactga ttttaactga gtttgagggc cgtatgttgc ctgttcccaa aaaatatgac
     8881 caatttttaa cccagatgta tggcgattat atgacaccac catcaaaaga aatgcaagag
     8941 tggtatagtc atagcattaa agcttatcgc aaaaactgat tgagggggat tatacagact
     9001 actaagatag aggttattca aaaacataat tttagtagaa aatgaaatac atattccca
//
LOCUS       DAVKJU010000074         8960 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVKJU010000074 DAVKJU010000000
VERSION     DAVKJU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8960
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.074"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(301..1908)
                     /locus_tag="VW191_001796"
     CDS             complement(301..1908)
                     /locus_tag="VW191_001796"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU9624524.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2298..2885)
                     /locus_tag="VW191_001797"
     CDS             complement(2298..2885)
                     /locus_tag="VW191_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEU9624525.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3013..3462)
                     /locus_tag="VW191_001798"
     CDS             complement(3013..3462)
                     /locus_tag="VW191_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU9624526.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGMSFILELV"
     gene            complement(3467..3856)
                     /locus_tag="VW191_001799"
     CDS             complement(3467..3856)
                     /locus_tag="VW191_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEU9624527.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3884..4534)
                     /locus_tag="VW191_001800"
     CDS             complement(3884..4534)
                     /locus_tag="VW191_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU9624528.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4524..5087)
                     /locus_tag="VW191_001801"
     CDS             complement(4524..5087)
                     /locus_tag="VW191_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEU9624529.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5252..5486)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5540..6043)
                     /locus_tag="VW191_001802"
     CDS             complement(5540..6043)
                     /locus_tag="VW191_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEU9624530.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKIYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6236..7066)
                     /locus_tag="VW191_001803"
     CDS             complement(6236..7066)
                     /locus_tag="VW191_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9624531.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(7051..8733)
                     /locus_tag="VW191_001804"
     CDS             complement(7051..8733)
                     /locus_tag="VW191_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624532.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LISDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELAKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2705 a         2059 c         1631 g         2565 t
ORIGIN      
        1 aaaatatttc tataaattaa tttgactttt ctgatagaga tgttcacatc ttatttcaat
       61 tcactataca atccagttcc taaaactttg ctagaaattg atttggcttt ccgaattaat
      121 ttgttcaaat tttatttcat ttcaatatac tgataaatcc attcactagc acagggcaag
      181 agggagtcgc tattgatttc aacggtagca ggtacataca gaggacccct acctcagata
      241 tactgcaata tcatcgtatg caaaaaacct ctgagatttt tctcaaaggt tctgattttg
      301 ctaattgctg ttctcaaccg ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt
      361 tggacggctt gacagttcag ggtgatactg acaagctaca aagaatttat tttcaggaat
      421 ttccacgatt tctaccaaac gattgtctgg agaaactcct gaaaagacaa aacctgctgc
      481 ctcaaactgc tcacggaagg cattattaaa ctcataacgg tgacggtgac ggcgttgcac
      541 cacttcttga ttgtgataag cagcagcagc cttagagcca cgtttcaact tagacggata
      601 aagtcccaaa cgaagggttc cacccatatc ctcaatatca atctgatcac gcatgatatc
      661 aatgataggg tattttgttt ctggtgcaag ctctgcagaa ttggcacctt caagacctaa
      721 aacgtgacga gcaaactcga tacatgtcaa ctgcattccc aagcagactc ccaacattgg
      781 aacatcattt tcacgcgcat agcggatggc ttggattttc ccttctgtac cacgttgacc
      841 aaaaccacct ggtacgatga tcccgtccgc atcagacaag agttctgcta cattctctgc
      901 tgtcacatca ttggcattga cccaattgat tttaacttct acatcattga catagccaga
      961 gtgtttcaag gcttcgacca ctgagatata ggcatcttgc aactccacat acttaccaac
     1021 aagggaaatc ttaacttgtt tcttgaggtt catgaccttg tccaccatgg ctgaccattc
     1081 tgtcatatcc gctgctggtg cgtctaattt caaatgatca caaacaattt ggtccatccc
     1141 ttgtgcctgc aagttcagtg gaatttggta aaggtgttca acatccaacg attcgataac
     1201 ggcttctggt gccacatcac agaactgggc cagtttattt ttaattcctt gaccagctgg
     1261 ctcttctgta cgaataacca acatatttgg ttggattccc aagccacgca attctttgac
     1321 agagtgttgg gttggtttgg ttttcatttc accagcagcc ttgaggtaag gaagcaaggt
     1381 tgtatggata tacatgacat tatccgcacc cacatctgcc ttcatctgac gaagagcctc
     1441 taggaatggc aaggactcga tatctcctac tgttccacca acctctgtga taatgacatc
     1501 agagtcggtc gttagagcgg cacgcttgat tttttctttc aaagcatctg tgatatgagg
     1561 aatgacttga acagttgccc caaggtattc tccacggcgt tctttacgaa gaacttcact
     1621 gtaaattttc ccagttgtca cgttggaata tttgttgaga ttgatatcga tgaaacgttc
     1681 atagtgaccc aagtccaaat ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg
     1741 ctggtaagga ctcatggttc ccggatcaat attgatataa gggtcaaact tttgaatggt
     1801 tactttgaga ccacgatttt tcaagagacg gcctagactc gctgccacaa tccctttccc
     1861 aatggacgat accacaccac cagttacaaa aatatatttc gtagacatag attcctcttt
     1921 ctaaaatgct caaggtcttg ttaactacga ggggatatag aaaacaagac cctactaata
     1981 agaataatct gggacgactg caccagattc acaactaata ctcttcgaaa atctcttcaa
     2041 accgcgtcaa cgtcgccttg ccgtagatat gtgttcctga ctttgtcagt cttatctaca
     2101 acctcaaaac agtgttttga gcagcctacg gctagtttcc tagcttgctc tttgattttc
     2161 attgagtata aaatacaaga agataacttc ttgaaaaaac aaaaatagct ccctaagaac
     2221 tagggagccc cgacctctaa aagaggtgcc caaacaatat gatacctaaa aataggataa
     2281 ttgtcaatag ctaactctta ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg
     2341 tcgtcttcgt tgagatccac atcttctccc aagtcatctg gagcgatttc gttgatttct
     2401 gcatcataag cttccacttc atttttttca tcatctggat tttcatcatc gtatgaaaga
     2461 gctggatctg cttcgtatgc atcttcgtct tctggatcat ctgcattgta gtcaatggca
     2521 tctgaatcac catccataaa ggcattaaca cgttttttct tagcttttgg tgctacttca
     2581 tcgtcgtcat tttcttcaag agcgatgatt tcttcgtcga tttcgtccac accataccat
     2641 gaacgaagac cccatttgtt gtccccaagt gagatgaagc taccgtcaaa gttcaactct
     2701 gtgtagaaca aaggcaaagc ttcgcggata tcgctgtttg atgttccaag gtagttttga
     2761 atttcgttta caagatcgct aaaatgcatc tcatgatcgc gaccacgaag ttccaagata
     2821 gcacgcgcta cctcaatcat agatagttca cttttttctt gcccagcaaa tacttctaat
     2881 tccaaagcgt ttctcctcat ttatactact atcgccagag cgaacagact ctgacctcat
     2941 tttatcattt actctttatt ttacgataat tttgcggaat agtcaaaggt taagggggag
     3001 aaagtggcag gattagacta attccaatat aaaactcatt cctttttctg ttgctccatt
     3061 ttccacaaat ccaagcgact tgaaacacct cctagaagca tgattgtagg tgtagatttc
     3121 cttgactctc aattctttcc atccttttac tcgagccaat tcaatcaaag cacttagaat
     3181 cttttttcca agtcctcgat gttggtaagc ggaattccca atcacaatgg ggagattatc
     3241 ctgagatagt gtaatatccc caattggaaa ccattctccc ttctccttga cttcaatcca
     3301 aaaaagctca ccatgtcgat ccaaatagga atacatggct tccaaggtcg cttgactgta
     3361 aggaagcttc accccatcta cgaggtaaac caagttcaca tcctgatacc aagcaaaagc
     3421 ttcctcacaa tgattgacct tataataagg aacaaaacac aatgcactac cttttcaaca
     3481 aaagagttgc tgcttgatta aaaccatcac accagttata ccattttgct tcatacccat
     3541 cttgagctag gatacgatct tctaaatcaa aaacagagta aatctttctt tcctcgcaag
     3601 cttgcgcata gagatgatat agttcatcac caccatctct atcccactca gcagaaatcg
     3661 tatcccgacc tgccaataaa gcctgataag ccctgtgatg cccatctgta atcagcaaac
     3721 aatctccaaa ggcaagaata ctgattggat cgacttggtt tgtttctgcc gactggtaaa
     3781 gcatctgaat atcttgcaac ttcttttctg ataaatatag ttgagtcgga tgaagatcta
     3841 ctatattgac tttcatttct ttctcctcaa gggaattcga tactcacttc tgtttgcctt
     3901 taaatcgcca ttggaagcgg agcttgtcat agaagggaaa ctcgataaac aagactccca
     3961 aacccacaca gaaactggca agaacatctg atgggtagtg aactcccaga tagactcttg
     4021 ataccagcac actgactagg tagaggccaa ggacgatttg tacgattttt ctccagacct
     4081 gatctttaat ccgctgacta agaataacaa tcaaagtccc taccatcagc gttacagcta
     4141 gagaatgccc acttgggaag gaaaatccct tctcctccac cagatgtaag atagctggtc
     4201 gtgggcgctg gtagatattt ttaaaggtca cgattaaaag acctgccaaa gccagatttc
     4261 ccagcatgaa gaaactttct atcttccatc gcttacgata aaagacaaaa gctataatga
     4321 caacccaagt gataatcact ggaatatcaa tcagatgtgt gagggatcga aaaagaatag
     4381 tcaaataatc tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaaccgacca
     4441 gcgtttcagg gtaaaatttg accatatagc caagaataac gaaaagtaaa agggcaaaac
     4501 tgcccttcat taaaaatgtt tgtttatctt tcatagtgtt ttaaggttgg tttcaaaagg
     4561 acgtacaaca accagaatga aacggaaaag attacaccct caatcaagtt aaaaggtaat
     4621 accatggtca ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac
     4681 ttagcgtaca aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca
     4741 acagttccag ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc
     4801 aaagcaaata cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa
     4861 gtattcactc cttggctgtt aagaagcaat ttcaagagtg agcgaagcaa gagcactcct
     4921 agagcagcag gcaaatccat gaccaccaga cccacaagga ctggcaagat actaaattcg
     4981 atcttgagga aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag
     5041 atggctgata gaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc
     5101 aattttctat aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat
     5161 atcccaaaag ttccctctta ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc
     5221 tataaatcta tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg
     5281 tcggttgtgg aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaagaaa
     5341 tagagatttt tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag
     5401 cttgtcaaca aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg
     5461 aatcacaccc tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca
     5521 ttttgttcat tttcattttt tatttcaatt tatccacttc ataggtatgg acgagctcaa
     5581 gacctgcaaa gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca
     5641 cattgagact gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt
     5701 ctcgcataaa atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct
     5761 cgtcagtcga taaatccacc tcagaataga ttttttccgc gaatttcgaa atcagataga
     5821 gttttccctt catctgagat atgaaatctt ctaaactctc gtaaaaataa atctctagct
     5881 tatcccagta atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag
     5941 gcttgatgat gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcctgtat
     6001 tttgtggaat ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac
     6061 cattgcaaaa aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc
     6121 atcgttaact tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata
     6181 acactttcag aatcattgtc aatagaaatg acttgatttt ttcaattttt tcaagctatt
     6241 tccaagggtt gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa
     6301 acaaggttgc caagagcaag gtaatatagt ctcctttttt caaggcctga taactatacc
     6361 atgtgcgttt tttctctttc ccaaagcggc gaagctccat ggcagtcgca atggtatcaa
     6421 tgcgttctag cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt
     6481 gcataagaga agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag
     6541 taaagaattc ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa
     6601 tcttataggg cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca
     6661 tcaaaaagat aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat
     6721 aaaagagctc ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct
     6781 ctccataaag tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa
     6841 cggcaaatac cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata
     6901 aatagaggag aaagactgaa aagatggcaa tcagcaagag cattctggta tcatagctaa
     6961 tcatggccgc caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat
     7021 gaatcacagt atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc
     7081 tttgtaaaat gccgttaaat ccagtggatc cacatctagt ttcttagcca agttaaagat
     7141 ggaggtttct tttagattgg cttttactaa cagctcagga tcgctcaaca gactggctgg
     7201 aacagtatcg gcaatcaatt ctccatccac catgacaagg acccggtctg aataatccag
     7261 catcaattgc atatcatggg taatcatgac aatggtatgc cctttttgat gtaactcttc
     7321 gagaaattcc ataatctcag tatagttctt ctgatcttga cctgcagtcg gttcatctag
     7381 gagaataatt tcagctccta agaccaaaat tgaagcaatg gtgacacgtt ttttctgacc
     7441 aaatgacagg gcagaaatag gccaattacg gaattcataa agtccacaga ttttcaaggt
     7501 ttcatagact ctcgtttcaa tttccttctc atccacacct cgcaaacgga gccctagagc
     7561 cacctcatca aaaatcatat tggttgaaat catttgatta ggattttgta gcacatagcc
     7621 tactcgttcc gcccgctctg caacagaatc gccttttata tcctgttttt cccaaagata
     7681 gcgtccttcc gtctgaataa agctacttat agccttggct agagttgatt tccctgctcc
     7741 attttttccg acaatagcaa tcttttcacc ctttttaata tctaaatgta gggattttaa
     7801 aatcggtcta tcatcataag agaaagacac gtcctctaat ttaaagagtg actgcaattc
     7861 tggagtttct tttgccagtt cattctgcag ctgaacctga ccttttgaga tagacatgtt
     7921 atccagattc gctaactgtt cttccttgac taagtccaca cctaattgac ggagagtcgt
     7981 tagataaagg ggttctcgaa ttccattttg agtcaataaa tcagtcgcaa gcaactggtc
     8041 agggctccca ttaaaaagga tacgaccatc gcttatcaag acaatccgat ccacagggcg
     8101 atgcagaaca tcctccaaac ggtgctcgat aatcagagtc gtcgtcccct cttccttatg
     8161 aatctggtca atcaattcga taatatcctg acctgacttg ggatctagat tggcgagtgg
     8221 ctcatcaaac aagagaatcg gactttcatc aatcaagaca ccagccagac tgactcgctg
     8281 cttttgtcca cctgacaaat cctgaggacg ctgatccagt aaaggaagaa ggtccagctt
     8341 ttcagcccat ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag
     8401 agcaaacgcc aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa
     8461 aactgtgcta accagatgag acttatcata gatgctcata tcaaaggcta cttgaccctt
     8521 tatcaaaaat tctccatatg tctgaccctt gtaaatattg ggaataatcc cattcaaaca
     8581 ctgacccaag gtagatttac ctgacccaga tggtccaaca attaagactt tctctccctt
     8641 gtaaatggtc aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga
     8701 gaaatccttc cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac
     8761 ttctatttta tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca
     8821 aaaagattcc tatcctagct tacttgccta actaatctat aaacatcgaa aaagactagt
     8881 tgcccagcct tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc
     8941 gccttgccgt aggtatggtt
//
LOCUS       DAVKJU010000075         8823 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVKJU010000075 DAVKJU010000000
VERSION     DAVKJU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8823
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.075"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            374..3649
                     /gene="rexB"
                     /locus_tag="VW191_001805"
     CDS             374..3649
                     /gene="rexB"
                     /locus_tag="VW191_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEU9624533.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMIFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGAEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPCVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3646..7296
                     /gene="addA"
                     /locus_tag="VW191_001806"
     CDS             3646..7296
                     /gene="addA"
                     /locus_tag="VW191_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEU9624534.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAANELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNIHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLINAQKMAS
                     SQKGLIHTALSEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     HLQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7511..8689
                     /locus_tag="VW191_001807"
     CDS             7511..8689
                     /locus_tag="VW191_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624535.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSIC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDYRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         2720 a         1855 c         1877 g         2371 t
ORIGIN      
        1 ttcaatcatt ctatcaagaa tattgaatat ataaaggcac gtaatattaa aacttcttct
       61 gatatcgaat tagagtccga tgttgatggc gataaatcag acaatctacc cgtcgacatt
      121 aagatactgg gacaacacat tgaagtgtat tccgtaccta agaaacaatc ataaaactca
      181 aagccttgtt accgaggctt ttttattatg tgattttcag tagccagact tattctatct
      241 aatacaagta aagctaagta tttaagattt agcagatgat gaagaaaaga aaggatggcc
      301 taaatatttt aacagattta cgaggctaat catggtataa tatagggtga tgaaaaattc
      361 caacgaggct gagatgaaat tactttatac tgatattcga acttctttga cagaaattct
      421 aacaagagag gcagaagagc tagttgcagc tggcaagcgg gtcttctaca ttgcccccaa
      481 ctctctttct tttgaaaagg aacgcgccgt gctggaatgc ttgtcccagc aggcttcttt
      541 ttcgattacc gtcacgcgct ttgctcaaat ggctcgttat ctggtcttga atgatttacc
      601 agctaaaact actcttgatg atatcggtct tgggttggcc ttttacaaat gccttgccga
      661 actcgatccc aaggacttgc gtgtttatgg cgctattaag caggatcctc aattgatcca
      721 gcagttaatt gagctttacc atgagatgac caaatctcag atgatttttt tggacttgga
      781 gaatttaaca gatgaggata agagggcgga tttactcttg atttttgaga aagtaacagc
      841 ctatcttaat caagggcagt tagcccaggg aagtcagttg tcccatttga ttgaggctat
      901 tgagaatgac aaggtaagta gtgattttaa tcaaatcgcc ttggtcattg acggctttac
      961 tcgtttttct gctgaggaag agcgggttgt ggacttactt cacggcaaag gtgctgagat
     1021 tgttatcggg gcttatgcta gtaagaaagc ctataccagt ccttttagcg agggcaatct
     1081 ctaccaagcc agcgtaaaat ttctccatca tctggcttct aaataccaaa cgcccgctca
     1141 ggactgttct caaactcatg agaagatgga tagttttgac aaggcctctc gtttgttgga
     1201 gtcttcttat gacttttcag aactcgcttt ggatgtcgat gagaaagacc gtgaaaattt
     1261 acaaatctgg tcttgtttga cgcaaaagga ggagttggag ctagtagccc gtagcattcg
     1321 tcagaaatta catgagaact cagacctgag ctacaagcat tttcgtattc tcttggggga
     1381 tgtagcttct taccagttat ctctcaaaac catttttgac cagtatcaga ttccttttta
     1441 tcttggtaga agcgaagcca tggctcatca tcccttgact cagtttgtcg agtctatttt
     1501 agctttaaaa cgttaccgtt ttcgtcagga ggatttgatt aatcttctta gaactgactt
     1561 gtatactgac ctcagtcagt ctgatattga tgcttttgag caatatatcc gctatcttgg
     1621 tattaatggc ttgccagcct ttcagcaaac cttcaccaaa tcccaccatg gaaaatttaa
     1681 tcttgagcgt ttgaatgtcc tccgcctgag aattttagca cctcttgaaa ccctctttgc
     1741 cagccgaaaa caaaaggctg aaaacctcct acaaaaatgg agtgtctttc taaaagaagg
     1801 agctgtgacc aagcagttac aagatttgac aaccactttg gaagctgtag aacaggaaag
     1861 acaagccgaa gtttggaagg ctttctgcca tgttttagaa caatttgcga ctgtttttgc
     1921 tggttcacag gttagtctgg aagacttcct agccttgctc cattctggaa tgagtttgtc
     1981 ccaataccgt accattccag caacagtgga cactgttctg gtgcagagtt acgatttgat
     2041 tgcaccattg actgctgact ttgtctatgc tattggacta actcaggaca atttaccaaa
     2101 aatttctcaa aacaccagtc ttctgacaga tgaagaaagg caaaatctaa accaagcgac
     2161 cgaagaaggc gttcaattac tgattgccag cagtgaaaat ctcaagaaaa atcgctacac
     2221 tatgctttcc ttggtcaatt ctgctcgtaa gcagttgttc ttgtcggctc caagcctttt
     2281 taacgaaagt gaaagtaagg aatctgccta tcttcaagag ttgatccatt ttggatttag
     2341 gcggagagag aagaggatga atcacaaagg actgtctaag gaggatatgg ggtcctatca
     2401 cagtcttttg tctagtctgg ttgcctatca ccagcagggt gagatgagcg atactgagca
     2461 agatttgact tttgtcaagg ttctgtcgcg tgtcataggt aaaaaactag atctgcaagg
     2521 tctggaaaat ccagccatcc caatcagtcc aagcagcaag accttagcca aggacacctt
     2581 gcaagctctc tatcctgcca aacaggagtt ttacctgtct acgtcgggtt tgacagagtt
     2641 ttatcgcaat gaatacagtt atttcctacg ctacgtttta ggcttgcagg aggaattacg
     2701 tttgcgtcct gatgcccgta gtcacgggaa tttcttgcat cgtatctttg aacgcgcctt
     2761 gcagttgcct aatgaagatt cctttgacca acgtctagaa caagctattc aagaaaccag
     2821 tcaagaacgc gaatttgaag ctatttatca agaaagtttg gaagcccagt ttaccaagga
     2881 agttttgctt gatgttgcac ggacaactgg acatattctc cgacacaatc cagccatcga
     2941 aacaatcaaa gaagaagcaa attttggtgg aaaagaccaa gcctttattc aattagacaa
     3001 tggacgcagt gtctttgtac gaggcaaggt ggaccggatt gaccgtttga aagctaatgg
     3061 agcgatagga gtagtagact acaaatccag tctgactcag ttccagtttc ctcatttctt
     3121 taatgggctc aattctcagt taccaaccta tcttgctgcc ctaaaaagag aaggggagca
     3181 gaactttttc ggcgccatgt acttggaaat ggctgaacct gtccaatctc tgatggcggt
     3241 aaaaagtctg gcaggagcag tggtagaagc cagcaaatct atgaaatacc aagggctctt
     3301 cttggaaaaa gaaagcagtt atttaggcga attttataac aaaaacaagg cgaatcaact
     3361 gacagatgag gaatttcagc tcctactgga ctacaatgcc tatctttaca agaaagctgc
     3421 tgagaagatt ttagcaggcc ggttcgccat caatccttat actgaaaatg gcagaagcat
     3481 tgctccatgc gtccagcaac atcaggctat tacaggcttt gaagccaatt accatctggg
     3541 ccaagcccgt ttcctagaaa agttggacct agctgatggc aagcgtctgg tcggagaaaa
     3601 actcaagcaa gcttggcttg aaaaaataag agaggagttg aatcgatgaa gcttattccc
     3661 tttttaagtg aggaggagat tcaaaaactg caagaagcag aagcaaattc gagcaaggaa
     3721 cagaagaaaa ctgccgagca aatcgaagct atctacactt ctggccagaa tatcctggtc
     3781 tcagcatcgg ctggttctgg aaagaccttt gtcatggcag agcgcattct ggaccaattg
     3841 gcgcgtggtg tcgaaatttc tcaactcttt atctcaacct ttaccgtcaa ggctgcaaat
     3901 gaacttaaag aacgtttaga gaaaaaaatc agcaagaaaa tccaagaaac agatgatgtc
     3961 gacctcaaac aacacttggg acgccagttg gcagacctac caaacgctgc catcggaacc
     4021 atggactctt tcacacaaaa attccttggc aaacatggtt atctgcttga tattgcacct
     4081 aatttccgta ttttacaaaa ccaaagcgag caacttcttc tcgaaaacga agtctttcat
     4141 gaggtctttg aagcgcatta ccaaggtaaa cagaaagaga cctttagtca tttgctgaaa
     4201 aactttgctg ggcgtggcaa ggacgaacgg ggtctgcgcc agcaggtcta taaaatctat
     4261 gacttcctcc aatccaccag caatcctcaa aagtggctga gtgactcttt cctcaaaggc
     4321 tttgagaaag ctgattttac cagtgaaaaa gaaaaactga ctgagcaaat caaacaagcc
     4381 ctttgggatt tggaaagctt tttccgttac catctggata acgatgccaa ggagtttgca
     4441 aaggctgcct atttagaaaa tgttcagtta attctggatg aaattggctc cctaaatcag
     4501 gagtccgata gtcaggctta tcaggcagtg cttgcgcatg ttgtcgccat ctctaaggag
     4561 aaaaacggtc gagctctgac taatgccagc cgtaaggctg atttgaagcc cctggctgat
     4621 gcctacaacg aagagagaaa gacccagttt gctaaactag gacaattatc agaccagata
     4681 accattctcg actatcaaga acgttatcat ggagacactt ggaaactagc taaaaccttc
     4741 caatctttca tgagcgattt tgtggaggct tatcgtcaga gaaaacgaca ggaaaatgcc
     4801 tttgaattcg ctgatatcag ccattacacc attgagattt tagagaattt cccacaagtt
     4861 cgtgagtctt atcaggagcg cttccatgaa gtcatggtcg atgagtatca ggataccaac
     4921 catattcaag aacgaatgct ggaattgttg tctaatggcc acaatcgctt tatggtggga
     4981 gatatcaagc aatccatcta tcgtttccgt caggcagacc cgcagatttt caatgagaaa
     5041 ttccaacgct atgcgcaaaa tcctcaagaa ggcaagctca ttctcctcaa ggaaaatttc
     5101 cgtagtagtt cagaagtgct gtcagcaacc aatgatgtct ttgaacgtct catggaccaa
     5161 gaggtcggcg aaatcaacta tgataacata caccagcttg tttttgccaa taccaaactg
     5221 actcccaatc cagacaacaa ggccgaattt ctcctctacg acaaggacga tacaggtgag
     5281 gaagaagaga gtcaaacaga aacgaaacta acaggcgaaa tgcgcttagt tatcaaggag
     5341 atccttaaac ttcatcagga aaaaggtgtt gcctttaagg aaattgctct tctgacctct
     5401 agtcgtagtc gtaatgacca aattctactt gccctgtctg agtacgggat tcctgtcaaa
     5461 actgacggag agcaaaacaa ttatctccaa tccctagaag tgcaagtcat gctagacact
     5521 cttcgtgtca ttcacaatcc cctgcaagac tacgccttgg ttgcccttat gaagtctcca
     5581 atgtttggtt ttgatgagga tgagctagca cgtttgtccc ttcagaaagc agaggataaa
     5641 gtccacgaaa atctctatga gaaactgatc aatgcacaaa aaatggcaag tagtcaaaaa
     5701 ggcttgattc acacagctct atctgaaaaa ctaaagcaat tcatggatat cctagcttct
     5761 tggcgcttgt atgccaaaac ccattctctc tatgacttga tttggaagat ttacaacgac
     5821 cgtttttatt atgactatgt tggggctttg ccgaatggtc ctgctaggca ggccaatctc
     5881 tatgccctag cactgcgtgc tgatcaattt gaaaagagca atttcaaagg tttgtcgcgt
     5941 tttattcgta tgattgacca agtcttagaa gcccagcacg atttggcaag cgtggccgtc
     6001 gcaccgccaa aagatgcagt agagctcatg accatccaca agagtaaagg gctggagttt
     6061 ccttacgtct ttatcctcaa tatggatcaa gatttcaaca agcaagactc tatgtcagaa
     6121 gtcattctca gtcgtcagaa tggtcttggt gtcaaatata ttgccaagat ggagacaggg
     6181 gcagtagaag accactatcc taaaaccatc aaactctcca ttcctagtct gacctatagg
     6241 cagaacgaag aggaattaca gctagcaagc tattctgagc agatgcgttt gctgtatgtt
     6301 gctatgacgc gggctgagaa aaagctctat cttgtcggca agggttctcg tgaaaagctg
     6361 gaatccaagg aatacccagc agccaaaaat gggaaactaa atagcaatac tagactgcaa
     6421 gcacggaatt tccaagattg gctttgggct atcagtaaag tgtttactaa ggacaagctc
     6481 aactttagtt atcgttttat tggcgaagat cagttgacca gagaagctat cggagagttg
     6541 gaaaccaaga gtcctctcca agatagctcc caagcagaca atcgtcagtc agataccatc
     6601 aaagaagctc tggaaatgct gaaggaggtg gaagtttata atactcttca ccgcgcagct
     6661 attgaacttc ctagtgttca aaccccaagt caaatcaaga aattctacga accagttatg
     6721 gatatggaag gtgtcgagat tgctggtcaa ggtcagtcag taggcaagaa aatcagcttc
     6781 gatttgccag atttttcaac caaagaaaag gtaactggag ctgagattgg tagtgctact
     6841 cacgaactca tgcagagaat tgacctcagc cagcaactaa cccttgctag cctaacagaa
     6901 acactcaaac accttcaaac tagccaagct gtcagagaca agatcaatct tgataaaatt
     6961 cttgctttct ttgacacagt actcggtcag gaaattcttg ctaataccga ccatctctat
     7021 cgcgagcaac ctttctccat gctcaaacga gaccaaaaga gtcaggaaga ctttgttgtc
     7081 cgtggtatcc ttgatggcta tctgctttac gaaaacaaaa ttgttctgtt cgactacaag
     7141 acagaccgct atgatgaacc aagtcaactc gtagaccgct atcgtggtca gttagctcta
     7201 tacgaagagg ctttatcacg agcctattcg attgaaaata ttgaaaaata cttgatttta
     7261 ctcggtaaag acgaggttca agttgtaaaa gtataactta tactctttga aaatctcttc
     7321 aaaccacgtc agcttcgcct tgctgtatat atggttactg acttcgtcag ttctatccac
     7381 aacctcaaaa cagtgttttg agctgacttc gtcagttcta tctacaacct caaaacagtg
     7441 ttttgagctg actgcggcta gctttctagt ttgctctttg atttttattg agtattagaa
     7501 aggagacctc atgacacttc cagttagaaa atccctgcac gatgcagttt tacaggcttc
     7561 gaaagcggat acttgggaac aagctaccaa ggaatggaat gaagtttcct tgatttttaa
     7621 cggtatcggc cgtagcattt gtgtctgtgg aaatgtcatc aaatacgctt atgaactctt
     7681 taacggtgtc acaggccaac gcctctttcc aattggtagc gactgtgttc gtcattttca
     7741 tcgattgtcc cttgaccagc aattggaaga ggaagaaaaa ctgctcagaa aggttgaaaa
     7801 tctaaccaga aaagctcaga aaaaggaaaa aatcaaggtc aataaaagtg actttgacga
     7861 gcgacttcta aaatggctct gggaaaaagg tgttttcaaa cccaatcgtg gcaatcaatt
     7921 tgcgcctgag agagactacc agcttttcct agaagtcttt cagggaggaa gttggaccaa
     7981 ggcggaacca aagaaaaagg ctcggatgga agaagtcctt gaaaagtgta tcaaaccctt
     8041 tttacttggg aaatcggatg accaactcta ccttgtcaag ctaggcaagg agaaaattga
     8101 ctacgaacaa gaactccgta tccaggcaga gaaagaacgc aagaagaggg ataaaatcgc
     8161 caagcaatat gcagacaatc tggttctagc catgggaccc gcagagcgtg cttatcaaga
     8221 ttactttggc tttacagaga ccctgactca agaagaacgc aagtgggaaa aaattctttt
     8281 tggtaaaaat agagcagaac gggctatcaa ggccaaacaa taccaaaaag agctggaaaa
     8341 agaccaacga attgccagtc aggatccaat tgaaagaaaa cagaaacaga cttggctcct
     8401 caattcctat tttcgtgagc ttcctgaaga aaaagctcga ttttctaggc tcttattaga
     8461 atatcgaaaa aatggtgaag tacccttttc aactgaatac ctgtcagatc atctcatcga
     8521 ctttttctat aaaatgaaag cctttgaatt tgaaatcgca ccagaacaag tccgagattt
     8581 tctaaaaaaa agcctgcagg aagactatcg ctcatcagca caaggaagct ggatagaagg
     8641 aattctcctc aactgcctca aaccattttt agaacgatta gtgatatgag taacacctac
     8701 cgtgtaaatt tcatggtagg tgttattgta cccccaaata atataaaaca aagtatgaaa
     8761 gcgcatctca attttaagac ttttttgtaa attaagagta taattaaggt atactgcctt
     8821 ttc
//
LOCUS       DAVKJU010000076         8656 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVKJU010000076 DAVKJU010000000
VERSION     DAVKJU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8656
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.076"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..432)
                     /locus_tag="VW191_001808"
                     /pseudo
     CDS             complement(1..432)
                     /locus_tag="VW191_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            688..1533
                     /locus_tag="VW191_001809"
     CDS             688..1533
                     /locus_tag="VW191_001809"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU9624536.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(1680..2741)
                     /gene="dinB"
                     /locus_tag="VW191_001810"
     CDS             complement(1680..2741)
                     /gene="dinB"
                     /locus_tag="VW191_001810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU9624537.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            3135..5459
                     /gene="pflB"
                     /locus_tag="VW191_001811"
     CDS             3135..5459
                     /gene="pflB"
                     /locus_tag="VW191_001811"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEU9624538.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(5597..6859)
                     /locus_tag="VW191_001812"
     CDS             complement(5597..6859)
                     /locus_tag="VW191_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEU9624539.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGGFPSLIHLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQDFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFICYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGF
                     RNFENFKKRIFIALNIKKERTKFVLSQA"
     gene            complement(7124..8653)
                     /gene="rlrA"
                     /locus_tag="VW191_001813"
     CDS             complement(7124..8653)
                     /gene="rlrA"
                     /locus_tag="VW191_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEU9624540.1"
                     /translation="MLNKYIEKRITDKITILNILLDIRSIELDELSTLTSLQSKSLLS
                     ILQELQETFEEELTFNLDTQQVQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQ
                     SFNEFTQKEYISIATGYRVRQKCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFG
                     IEIYDLNDGSMDWVTHMIVQSNSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFP
                     ESKEFEKLKNLFMYPILMEHCQTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWN
                     QEKKTHTIQLILQHTRGKHLLSKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVP
                     YYNYYEHYGIESDKPLYHISKAIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLP
                     AIPIFIILNNQADVNLIKSIILRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTK
                     EYLPYVKKQYPKGKHHFLTIALDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYL
                     L"
BASE COUNT         2568 a         1604 c         1755 g         2729 t
ORIGIN      
        1 gcttaatttc tatcagctta tcaaatcctg cctcaagcct tttctgagga ttagggtagc
       61 atgtcaagag ttggtaggta tattctgaat gcttttcaac gattttatcc aactcaggaa
      121 agatgatatc aagacaacga gtgtattgta ctttccaatc agactgtttt ctcttgagac
      181 gatgaatatg tctagccagt atttttagtt ctacttgccg attatcgtgt tgaaattgtt
      241 cacgattggg gtcagaaaga agtttaagag cgatgagaca gtcttggttg agctgtcgaa
      301 aagacagatc taagagttca aaaccagctt tattatttga aaaagtgagt ggtttaagaa
      361 cagttttcct cgaacattca aggctgtaac atcgtgttta tttttagcga catcaatgcc
      421 cacataaagc atgggagtac ctccagatat agtatttcaa gtctacttgg ttatccacga
      481 actttttgcc ttgttacctt agacgagata aaacgtctat gcgttatcaa actcattacc
      541 aattgaaaca aaaagctgtg gttagaccct ttcggaaatc gtcaagcgat tggaggaaat
      601 gaactaatcc acagtggctt attccaagta taccacttgg gctttggcag tagctaactg
      661 cgttaaatat aatataagga gaaatatatg tatcttattg aaattttaaa atctatcttc
      721 ttcgggattg ttgaaggaat tacggaatgg ttgccgattt ccagtacagg tcacttgatt
      781 ttagcagagg agtttatcca ataccaaaat caaaatgaag cctttatgtc catgtttaat
      841 gtcgtgattc agcttggtgc tattttagca gttatggtga tttattttaa caagctcaat
      901 ccttttaaac cgactaagga caaacaggaa gttcgtaaga cttggagact atggttgaag
      961 gtcttgattg ctactttacc tttacttggt gtctttaaat ttgatgattg gtttgatacc
     1021 cacttccata acatggtttc agttgctctc atgttgatta tctacggggt tgccttcatc
     1081 tatttggaaa agcgcaataa agcgcgtgct atcgagccaa gtgtaacaga gttggacaag
     1141 cttccttata cgaccgcttt ctatatcgga ctcttccaag ttcttgctct tttaccaggg
     1201 actagccgtt caggtgcaac gattgtcggt ggtttgttaa atggaaccag tcgttcagtt
     1261 gtgacagaat ttaccttcta tcttgggatt cccgttatgt ttggagctag tgccttaaag
     1321 attttcaaat ttgtgaaagc cggagaactc ttgagctttg ggcaattgtt tttgctcttg
     1381 gtcgcgatgg gagtagcttt tgcggtcagc atggtggcta ttcgcttctt gaccagctat
     1441 gtgaaaaaac acgacttcac cctttttggt aaataccgta tcgtgcttgg tagtgttttg
     1501 ctactttaca gttttgtccg tttatttgta taagaaaaac cttgaagggg caactcttca
     1561 aggttttata ctcttagaaa atctcttcaa accgcgtcag ctttatctgc aacctcaaaa
     1621 cagtgttttg agcagcctgc ggctagcttc ctagtttgtt ctttgatttt cattgagctt
     1681 taaaatccag tcagggtaat tcccaatagg cggacacctc tttctttctc gcttaattct
     1741 tcatagagtt gcagggctat ttggcttatc tgactagcat cttgtgtttt ttgagcaaga
     1801 ctttttcgtt tggtaagagt tgaaaagtcc tcgtagcgga ttttcaaaat gacaattttt
     1861 ccagcttttt cttgttgatg tagattgaga gcgacttttt ctgatagaag agtcagctct
     1921 tttttgatat cttcctcagc acggagaatc ttcccgtagg ttttctcctt gccgattgat
     1981 ttacggatgc gattggattt gactggagag ttgtgaatgc cacgagcctt tcgatacaga
     2041 tcatagccta gtctaccaaa acggtctatt agggtcacct caggaacttc aagtaaatca
     2101 gcaccagtaa aaacgcccat ttgatgaaga cgttctactg tcttttttcc tactccatga
     2161 aatttggaaa tatccatttg tttgagaaaa tcctcagcct gttcaggtag aatcactgtc
     2221 aaaccatgag gtttttgata atcactcgcc attttagcta agaacttgtt ataggaaata
     2281 ccggcagaag cagttagatg gagttcttgc cagatatctt tttgaatgag gcgagcaatt
     2341 ttgaccgctg acttgatacc gagtttattt tctgtcacat ccaaataggc ttcgtcaata
     2401 ctcatgggtt caatcaaatc tgtatagcgc ttaaaaatag ctcgaatctg gagtcccaca
     2461 gctttgtatt tctcataatt tcctgagata aagacggcct ggggacaacg ttcataagct
     2521 tccttggaac tcatggcaga atggacacca aaagctcgag cctcatagct acaggtagaa
     2581 acgactcccc gcccacctgt ttgtcgaggg tcgcttccaa taatgacagg ttttcctctg
     2641 agtttaggat tatccttgat ttccaccgca gcaaaaaagg catccatgtc aatatggatg
     2701 atttttcttg acaaatcatt taacaaagga aaaatcaaca tgcctagcac ctttttatac
     2761 ttttcaaaaa tctcttcaaa ccacgtcagc ttccatctgc aacctcaaaa cagtattttg
     2821 agctgacttc gtcagttcta tttacaacct caaagcagtg ctttgagcag cctgcggcta
     2881 gcttcctagt ttgcttttcg atttccattg agtgttactg cttattttct tttattatac
     2941 ccttttttct gaaaaaaaga aaaaaggact ttattttttc aaaaatataa tacagtttga
     3001 aataaaatat agactgtttt agaaaagaaa gtgtaaaaat agggaatttt cacttgttga
     3061 aatcggttac tatatggtat acttgtctta tgaatgtaac agatgactgt tactagaaaa
     3121 aagaggacat taatatggtt gttaagacag ttgttgaagc acaagatatt tttgacaaag
     3181 cttgggaagg cttcaaaggc gtagattgga aagaaaaagc aagtgtatca cgatttgtac
     3241 aagctaacta cacaccttat gatggagacg aaagcttcct tgcaggacca acagagcgtt
     3301 cacttcacat caagaaaatt gtagaagaaa ctaaagcaca ctacgaagaa actcgtttcc
     3361 caatggacac tcgtccaaca tctatcgctg atatccctgc tggatttatc gacaaagaaa
     3421 atgaagttat cttcggtatc caaaacgatg aactcttcaa attgaacttc atgccaaaag
     3481 gtggtatccg tatggctgaa actactttga aagaaaatgg atacgaacca gacccagctg
     3541 ttcacgaaat cttcactaaa tatgtaacaa cagttaacga cggtattttc cgtgcctaca
     3601 cttcaaatat tcgtcgcgct cgtcacgcac acactgtaac tggtcttcca gatgcatact
     3661 cacgcggacg tatcatcggt gtttacgcac gtcttgctct ttacggtgca gactacttga
     3721 tgcaagaaaa agtaaatgac tggaatgcaa tcaaagaaat cgatgaagaa acaatccgtc
     3781 ttcgtgaaga agtaaacctt caataccaag cattgcaaca agttgttcgc ctgggtgacc
     3841 tttacggggt tgatgttcgc aaaccagcga tgaacgtgaa agaagcaatc caatgggtta
     3901 acattgcttt catggctgtc tgccgtgtga ttaacggtgc tgctacatct ctaggtcgtg
     3961 taccaatcgt attggacatc tttgcagaac gtgaccttgc tcgtggtaca tttactgaat
     4021 cagaaatcca agaattcgtt gatgatttcg ttatgaaact tcgtacagtt aaatttgctc
     4081 gtacaaaagc ttatgaccaa ttgtactcag gtgacccaac ctttatcaca acttctatgg
     4141 ctggtatggg taacgacggt cgtcaccgtg ttactaagat ggactaccgt ttcttgaaca
     4201 ctcttgacaa catcggtaac tcaccagaac caaacttgac agttctttgg actgacaaat
     4261 tgccatacaa cttccgtcgc tactgtatgc acatgagcca caaacactct tctatccaat
     4321 acgaaggtgt aacaacaatg gctaaagacg gatatggtga aatgagctgt atctcatgct
     4381 gtgtgtctcc acttgatcca gaaaatgaag aacaacgcca caacatccag tacttcggtg
     4441 ctcgtgtaaa cgttcttaaa gcccttctta ctggtttgaa tggtggttac gacgatgttc
     4501 acaaagacta caaagtattt gatatcgaac caatccgtga cgaagttctt gaatttgaat
     4561 cagttaaagc gaactttgaa aaatctcttg actggttgac tgacacttac gtagatgcct
     4621 tgaacatcat ccactacatg actgataggt acaactacga agctgttcaa atggccttct
     4681 tgccaactaa acaacgtgcc aacatgggat tcggtatctg tggatttgct aacactgttg
     4741 atacattgtc agctatcaaa tacgctacag ttaaaccaat ccgtgacgaa gatggctaca
     4801 tctacgatta cgaaacaatc ggtgactacc cacgctgggg tgaagatgac ccacgttcaa
     4861 acgaattggc agaatggttg atcgaagctt acacaactcg tctacgtagc cacaaactat
     4921 acaaagacgc agaagctaca gtatcacttt tgacaatcac atctaacgtt gcttactcta
     4981 aacaaactgg taactcacca gttcacaaag gtgtatacct caacgaagat ggttctgtga
     5041 acttgtctaa acttgaattc ttctcaccag gtgctaaccc atctaacaaa gctaaaggtg
     5101 gttggttgca aaacttgaac tcactttcta gccttgactt tagttatgca gctgacggta
     5161 tctcattgac tacacaagta tcacctcgcg ctcttggtaa gactcgtgat gaacaagttg
     5221 ataacttggt aacaatcctt gatggttact tcgaaaacgg tggacaacac gttaacttga
     5281 acgttatgga cttgaacgat gtttacgaaa aaatcatgtc aggcgaagac gttatcgtac
     5341 gtatctctgg atactgtgta aacactaaat acctcactcc agaacaaaaa actgaattga
     5401 cacaacgtgt cttccacgaa gttctttcaa tggatgacgc cttggatgca ttgagctaat
     5461 caagttcttg aataataaaa aggaaccctc ggtcaaacga ctgagggttt tgtgcttggg
     5521 atagtatgag caattccttc ggcgcaatat gcaatgtttt tgggctcttt gtcaactgta
     5581 gtgggttgaa aaaaagctaa gcttgagaaa ggacaaattt cgtcctttct tttttgatgt
     5641 tcagggcgat aaaaatccgt tttttgaagt tttcaaagtt ccgaaaacca aaggcattgc
     5701 gcttgatgtc tttgatgagt ttgttagtgg cctcaagttt agcgttagaa taaggcaatt
     5761 caatggcgtt agtgatgtag tttttatagc aaataaatgt gctcaaagtg gttttaaagg
     5821 tgcggttgag atgaggtaac gtgtcttgaa ttaagcccca aaactggtca gtattcttct
     5881 cttgtagatg aaataggagt agttgataca ggtcatagta atctttaagt tcaggtacta
     5941 gagtaaagat tttcttcaga cactccctag gagttaaggt ctctctgaaa gttctagcat
     6001 agaaaggctt aagagagagt ttccgactat cttttaggat aaatttccag taatatttaa
     6061 gagctctgta ttccagagat ttatcatcaa attgcttcat gatgttgatt ctagtctgat
     6121 taagagccct gctcatgtgt tggacaatgt ggaaacgatc gagaacaatt ttagcattgg
     6181 gaaataattt cttaatgaga gggatataac ttccagacat atcaacagtg acgactttaa
     6241 ctttttttct agcttctttc gagtacttga agaaatgatt tcggatggtt gtttgacgtc
     6301 tgttatcaag aatggtcatg attttcttag tgttgaaatc ctgagcaatg aaagccaatt
     6361 tccccttctg gtaggagaat tcatcccagg agaggatttc aggcaaagtg gtgtaatcct
     6421 cttggaaatg aaattgcttg agcttacgat agacggtaga ggtagaggta gagatggcta
     6481 atttagaagc gatatgtgta agagcctctc tgttgagtag gagttgggca attttctgtc
     6541 tcaccatttc cgagatttgg caatttttct gaacgagagt tgtttcagct acagtgactt
     6601 tccgacagga cttgcattga aatcgtctct ttttcaaatg aatgaggcta gggaaaccac
     6661 caatctcgat aaaagggatt ttagaaggct tttggaagtc gtatttgatt tgttttcctt
     6721 tacagtgttt acatttaggt gggtgataat caagtgtagc gaagacttcg atatgggtat
     6781 cgtgctgaat ggctttattt aaggtgatgt ttttgtcttt tattccgatg agtaatgtgg
     6841 tatgattgat gtgttccata agatactttc taatgagttg tttaggcgct tttcattata
     6901 agtcttatgg gacttttttg atactcaaaa agccctataa tctccacagt gggatttacc
     6961 cactacagaa attatagagc cagaaaaaac acttttgttc actagcagaa actagagagc
     7021 agaagtgttt ttctgttcag atttacccaa aactgggaaa tatggggata agaatagaga
     7081 tggcttagga agcccctttt tgtgtgtaga cagtacgatg aacttataac aaatagtgag
     7141 cctttttagc aatcattgcg acccgtttgt caaaagcctc ttttcggata tctacaattg
     7201 tctgatagat gagacgctgt tggctaacat gcaaatctaa ggcaatcgtc aaaaagtgat
     7261 gtttcccttt gggatactgc tttttaacgt aaggcaggta ttctttcgtt gtaataataa
     7321 tcaatggctc tgtcaaatgc tcctctgaag gaggaggact aattagaata ttgtatcctg
     7381 taacagaggc aactttgtca gtaaaattcc gtaaaataat ggactttatt aagtttacat
     7441 ctgcttgatt atttaaaatg ataaaaatcg ggatagcagg tagtgaggaa aagatggttt
     7501 ctgtcaagta gagtgagaaa aggtacagcc gatgctggtc gataactcct tcaatcttct
     7561 gctcagtcat ccactcttga acaattgctt tcgaaatatg atacagtggc ttgtcgcttt
     7621 caatcccata atgttcgtaa taattataat agggaactag attttgtaaa ccaaacaaaa
     7681 acgttcttgt taagaaagtc agtgctgtta aaaaagaaag agaattcgaa atgtcatttc
     7741 ctaagatatt cttgaacttg gatagtagat gctttcctct tgtatgctga agaatcagtt
     7801 gaatagtatg agtctttttt tcttgattcc atttgtcctt ggaaaacgaa gaattagcag
     7861 aacaataaac caaaaagata taatccagtt cttcctgagt aaaagtcatg ttggcatgtg
     7921 gctctaagta agtttggcaa tgttccatca aaatcggata cataaagagg ttttttaatt
     7981 tttcaaactc tttggactca gggaactcaa gtggaaattc ccgacgtttc caagtgagtg
     8041 ccactagtat gctaaaatga acatactcgt caggtgtgat ttctaacagt tcatgactga
     8101 gttgagaatt agactgcaca atcatatgtg tgacccaatc catacttcca tcattcaaat
     8161 cataaatctc aataccaaaa tgaaactgga ggagtgcaat taaaaaacga atgcgatatt
     8221 caggaccaac tacttgattt ttcacaaggt ccaaacctac tgaacgtagt aacaagccac
     8281 acttttgtcg tacgcggtag cctgttgcga tggaaatata ctctttttgt gtaaattcgt
     8341 taaagctttg attaccttgt agtagaaaga agcggagtat ttttaaaata gttgattggt
     8401 tataaagctg atggaagtaa taattcgttt gatgagaatg gtgttcgatt aattgaactt
     8461 gttgcgtatc taaattaaat gtcaactctt cctcgaatgt ttcttgtaat tcctgcaaaa
     8521 tgcttaggag acttttagat tgtaatgaag ttaaagtaga cagttcatct agttcaatag
     8581 accgaatatc caataatata tttaaaatgg taattttatc tgtaattctt ttttcaatgt
     8641 atttgtttag catagt
//
LOCUS       DAVKJU010000077         8169 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVKJU010000077 DAVKJU010000000
VERSION     DAVKJU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.077"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            185..1666
                     /locus_tag="VW191_001814"
     CDS             185..1666
                     /locus_tag="VW191_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9624541.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDSLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2027..3028
                     /locus_tag="VW191_001815"
     CDS             2027..3028
                     /locus_tag="VW191_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU9624542.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3178..4095
                     /locus_tag="VW191_001816"
     CDS             3178..4095
                     /locus_tag="VW191_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU9624543.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4106..4996
                     /locus_tag="VW191_001817"
     CDS             4106..4996
                     /locus_tag="VW191_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU9624544.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5025..6641
                     /locus_tag="VW191_001818"
     CDS             5025..6641
                     /locus_tag="VW191_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU9624545.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6651..7970
                     /locus_tag="VW191_001819"
     CDS             6651..7970
                     /locus_tag="VW191_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEU9624546.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
BASE COUNT         2732 a         1294 c         1551 g         2592 t
ORIGIN      
        1 ttgattctgt attcacgccc tcaatagacg gtaaaataag aaaattgttt atatattgtc
       61 tccgtagtgt tattatacga aataaaagat tttaggaaaa gtcgttatat catgctatac
      121 ctattctttg tggtataatt gcaagaggtt taatccgata atttataaag agagaaagat
      181 attcatgaga gatttattat ctaaaaaaag tcataggcaa ttagaattat tagaattatt
      241 atttgaacat aaacgttggt ttcatcgttc tgaactagca gagttattaa attgtacaga
      301 acgtgcagtc aaagatgatc tatcccatgt taaatctgct tttcctgact tgatttttca
      361 ttcttctact aatggtatac gcattattaa taccgatgat agtgatattg aaatggttta
      421 ccatcatttc tttaaacatt caactcattt ttcgatttta gaattcatct tctttaatga
      481 aggttgtcaa gctgagagta tttgtaaaga attttatatc agttcatctt cgctctatcg
      541 tattattagc caaatcaata aagtgattaa aaggcaattt caatttgaag tcagtctgac
      601 ccctgttcaa atcattggaa atgagagaga cattcgttac ttttttgcac aatatttttc
      661 agaaaaatat tatttcctag aatggccatt tgaaaatttt tcatcagagc cactatctca
      721 attgttagaa ttggtttata aggaaacaag ctttccaatg aatttgtcaa cgcatagaat
      781 gctaaagttg ctcctagtta cgaacctata tagaataaag tttggtcatt ttatggaagt
      841 agataaagat tcttttaacg accaaagttt ggatttttta atgcaggcag aaggaataga
      901 aggtgttgct cagagttttg aatcagaata caatatctct ttagatgaag aagttgtttg
      961 ccaattattt gtgtcttact ttcaaaaaat gtttttcata gatgaaagtc tctttatgaa
     1021 atgcgtaaaa aaggatagtt atgttgaaaa atcttaccat ctattgagtg attttattga
     1081 tcagatttca gtcaagtatc agattgaaat ggagaataag gatagtctga tttggcatct
     1141 gcataatacc gcacatctgt atcgtcaaga gttgtttact gagtttattt tatttgatca
     1201 aaaagggaat acaatcagga actttcaaaa tatttttcca aaatttgttt cagatataaa
     1261 aaaagagctt tctcactatt tagagactct tgaggtttgt tctagttcaa tgatggtaaa
     1321 tcatctatct tataccttca tcactcatac caaacatttg gtgattaatt tgctacaaaa
     1381 tcagcctaaa ctaaaggttt tagttatgag taattttgat cagtatcatg caaaattcgt
     1441 tgcagagaca ctttcttatt actgtagcaa taattttgaa ctggaagttt ggactgaatt
     1501 agaattatca aaggaatctt tagaagattc atcttatgat atcattattt ctaattttat
     1561 tattcctcct attgaaaata agagactcat ctattcaaat aatataaaca cggtctcact
     1621 catatatttg ttaaatgcca tgatgtttat tcgattagat gagtaaccaa aaataacaaa
     1681 aaaacgctat ttcgcgtttg aactgtacct caaaagttag acagaaaaaa tctaattttt
     1741 ggggtgtttt tgttaagaaa agaccttcaa tcatttaagc aaaatgtaca tctagattta
     1801 tagccctcta aaactcttgc tttttttgta gaagatacaa aaaagccata aaaccaaggc
     1861 ttttcctgtt ttatgtagat gtttacacat gaaaattgaa gtattttcaa aagctaattg
     1921 gagtgatttt attcatgatg ggataaatga tctggccaaa aatgattgag aaatagttgc
     1981 ttaaaaagca tttgcatgat actatatatt gaaaggaggt gataatatgg tagaacaaag
     2041 aaaatcaatt accatgaaag atgttgcttt agaagcagga gttagtgttg gaactgtttc
     2101 acgtgtaatt aataaagaaa aaggcattaa agaagtaact ttgaaaaaag tggaacaagc
     2161 gattaaaact ttgaattaca ttccagatta ctacgctaga ggaatgaaaa aaaatcgaac
     2221 agaaacgatt gcaatcattg taccaagtat ctggcatccc ttcttttcag aatttgctat
     2281 gcatgtggaa aatgaagtct ataagagaaa taacaaatta ctcttatgtt ctatcaatgg
     2341 tacaaataga gagcaagact atctggagat gttgcgtcat aataaagttg atggagtggt
     2401 tgccattacc tatagtccaa ttgaacatta cttgacgtca ggaattccct ttgttagtat
     2461 tgaccgcaca tactcagata ttgccattcc ttgtgtttca tcagataatg atgcaggtgg
     2521 aagagaagca gctaaacaat taattagtaa aggatgtcag catttagcct ttgtgggcgg
     2581 acataataca accattaatg aaaccaaacg tcgtagaatt tcatttgaaa agtatgtcca
     2641 agagcataaa atacctctta gtatttttga tttggatgag acagttactg actattatgg
     2701 aaagctagag agtttcttgg aagaaaattc ttctatagat ggaattttta caatcaacga
     2761 ttttacagct ttggatgtga ttgaaatact aaaaaaaaat ggcaaacgta ttcctcatga
     2821 tgtccaaatt attggatacg atggaattaa aatggctgga gatagagatt atctactttc
     2881 aactattgaa caaccattgg aagaaatggc aaaagaagcg gttcgtattt tgtttgatat
     2941 aattgatgga aagactgtta atttgcagac aatcctacca gtaaaatttg ttgaaggaaa
     3001 aacaacaaaa aatgaaaata agtcttgaca gaaagcgcta tcaatgatag aatgaattca
     3061 tataaaaaga tttattttta aaacaaaaat gaaacgtttc aaaaaaagaa ataaagagac
     3121 agcgtcaagc gctatctttt ctagaacaaa aatgaaacgt ttcaaaagga ggttgctatg
     3181 aatagcaaag cgaagcaagt ttctctttgg gaaagaatca agaaacaaaa actcttgtta
     3241 ttgatgactg tccctggttt agttttaacc tttatcttta aatacatccc tatgtatggg
     3301 gttttaatcg catttaaaga ttacaatccc ttaaaaggaa ttttagggag tgattggatt
     3361 ggtttttctg agtttacaaa attcatatcc tctcccaact ttggtatctt gttagccaac
     3421 acattaaaat taagtatcta tggtttattg cttggctttt taccaccaat cattcttgct
     3481 attatgctca atcaactctt gagtgaaaaa gtcaaaaaac gaattcagct cattttatac
     3541 gcaccaaact ttatctcagt cgttgttatt gtcggtatga ttttcctctt cttttcagtg
     3601 ggaggaccaa tcaacaattt tctttctatg tttggaatga aggctgactt cttgacaaat
     3661 ccagacttct ttagaccttt atacatcttt agtggtatct ggcaaggaat gggctgggct
     3721 tcaacgctct acacggcaac attggtaaat gtagatccag ccttagtaga agcagctcga
     3781 ctggatggag ccaatatctt ccaacgaatc tggcacattg atattccagc tcttaagcct
     3841 attatggtta tccaatttgt tttagctgca ggtggaatta tgaatgtcgg atatgaaaaa
     3901 gcattcttga tgcagacatc gttaaatttg ccaacttctg aaattatctc gacatatgtc
     3961 tataaagttg gtcttgtatc aggagactat tcttactcaa cagcggttgg tttgtttaat
     4021 gcagtgatta acgtagtatt gcttgttgca gttaaccaaa tcgttaaacg catgaataat
     4081 ggtgaaggaa tttaaggagg gaagtatgaa aaattcgatt atggatacaa aatttgatag
     4141 acgtatctta ctcttaaata aaatcattat tgtctttatc gttttgatga ctttgcttcc
     4201 tttactttat atcgtcgtag catcctttat ggatcctaag gttctggtta gtagagggat
     4261 tagctttaat ccagccgatt ggactgtaga aggttatcag cgtgtattca gtgaccaatc
     4321 tattctaaga ggttttatca attctctact atactctttt ggatttgcag ctttaacagt
     4381 attgctatct gtgtttacag cttatcctct ttctaagaaa gacttggttg gacgtcgttg
     4441 gattaactac ttcttgattg tgactatgtt ctttggtggt ggtttagtcc caacttactt
     4501 gctcgtaaaa gaattgggaa tgctcaatac tccatgggct atcattgttc caggtgctgt
     4561 caacgtttgg aatattattc ttgctagggc ctatttccaa ggattgcctg aagaattagt
     4621 tgaagctgct gtcattgatg gtgcaaatga tttacagatt ttcttcaaaa tcatgcttcc
     4681 tcttgcaaaa ccaattatgt ttgttctctt cctctatgct tttgtaggac agtggaactc
     4741 atactttgat gcaatgattt atatcaagga tccaaacttg gaaccattgc aacttgtact
     4801 tcgtaaaatt ctcattcaga gccaaccagg tcaagacatg attggagcac aagcggctat
     4861 gaatgaaatg aaacgtttag ctgaattgat taaatacgca actattgtca tttccagctt
     4921 gccattgatt gttatgtatc cattcttcca aaaatacttt gataaaggaa ttatggctgg
     4981 ttcacttaaa ggataaaaaa agaaaaaata aaaggagttt tctcatgaaa ttcaaaacat
     5041 tctcaaaatc agcagttttg ttgacagcta gtttagcagt acttgcagcc tgtggctcaa
     5101 aaaatacagc ttcaagtcca gattataagt tggaaggtgt aacattcccg cttcaagaaa
     5161 agaaaacatt gaagtttatg acagccagtt caccgttatc tcctaaagac ccaaatgaaa
     5221 agttaatttt gcaacgtttg gagaaggaaa ctggcgttca tattgactgg accaactacc
     5281 aatccgactt tgcagaaaaa cgtaacttgg atatttctag tggtgattta ccagatgcta
     5341 tccacaacga cggagcttca gatgtggact tgatgaactg ggctaaaaaa ggtgttatta
     5401 ttccagttga agatttgatt gataaataca tgccaaatct taagaaaatt ttggatgaga
     5461 aaccagagta caaggccttg atgacagcac ctgatgggca catttactca ttcccatgga
     5521 ttgaagagct tggagatggt aaagagtcta ttcacagcgt caacgatatg gcttggatta
     5581 acaaagattg gcttaagaaa cttggtcttg aaatgccaaa aactactgat gatttgatta
     5641 aagtcctaga agctttcaaa aacggggatc caaatggaaa tggagaggct gatgaaattc
     5701 cattttcatt tattagtggt aacggaaacg aagattttaa attcctattt gctgcatttg
     5761 gtatagggga taacgatgat catttagtag taggaaatga tggcaaagtt gatttcacag
     5821 cagataacga taactataaa gaaggtgtca aatttatccg tcaattgcaa gaaaaaggct
     5881 tgattgataa agaagctttc gaacatgatt ggaatagtta cattgctaaa ggtcatgatc
     5941 agaaatttgg tgtttacttt acatgggata agaataatgt tactggaagt aacgaaagtt
     6001 atgatgtttt accagtactt gctggaccaa gtggtcaaaa acacgtagct cgtacaaacg
     6061 gtatgggatt tgcacgtgac aagatggtta ttaccagtgt aaacaaaaac ctagaattga
     6121 cagctaaatg gattgatgca caatacgctc cactccaatc tgtgcaaaat aactggggaa
     6181 cttacggaga tgacaaacaa caaaacatct ttgaattgga tcaagcgtca aatagtctaa
     6241 aacacttacc actaaacgga actgcaccag cagaacttcg tcaaaagact gaagtaggag
     6301 gaccactagc tatcctagat tcatactatg gtaaagtaac aaccatgcct gatgatgcca
     6361 aatggcgttt ggatcttatc aaagaatatt atgttcctta catgagcaat gacaataact
     6421 atccaagagt ctttatgaca caggaagatt tggacaagat tgcccatatc gaagcagata
     6481 tgaatgacta tatctaccgt aaacgtgctg aatggattgt aaatggcaat attgatactg
     6541 agtgggatga ttacaagaaa gaacttgaaa aatacggact ttctgattac ctcgctatta
     6601 aacaaaaata ctacgaccaa taccaagcaa acaaaaacta gaggttgatt atgggagata
     6661 agaaatacac agtagaaaaa gccaatcgtt ttatagcaga aaataaacat ctcgttaata
     6721 ctcaatataa gcctgaagaa catttttcag ctgagattgg ttggatcaat gatccaaatg
     6781 gatttgtcta ttttcgtgga gaataccatc tcttttatca attctatcca tatgatagtg
     6841 tttgggggcc tatgcactgg ggacatgcta aaagtaagga cttggtgact tgggagcact
     6901 tgccagtggc acttgctcct gaccaagatt atgaccgaaa tggttgcttc tcaggctctg
     6961 ccattgtcaa ggatgatcgc ctctggctca tgtatactgg gcatatcgaa gaagaaaccg
     7021 gtgtccgcca agtgcaaaat atggcatttt cagatgacgg gattcacttt gaaaagattt
     7081 cccaaaatcc agttgcaact ggagcagact tgccagatga gttgattgct gctgatttcc
     7141 gtgatccaaa actctttgaa aaagatggac gctattactc cgtagtagct gccaaacaca
     7201 aggataatgt gggctgtatc gttctactag ggtccgataa cctagtagaa tggcagttcg
     7261 aatccatctt tttaaaaggg ggagaacacc aaggctttat gtgggaatgc ccagattact
     7321 tcgagttaga tgggaaagat tgccttatta tgtcacccat gcgttatcag cgtgagggag
     7381 actcatatca taacatcaac tcatcgcttt tgttcacggg taaggtagat tggggagaaa
     7441 aacgttttat cccagaatca gttcaagaaa ttgatcatgg ccaagacttc tatgcgcctc
     7501 aaacattgtt ggacgatcaa aatcgtcgta tcctgattgc ttggatgcag acatgggggc
     7561 gtacccttcc aacccatgac caagaacaca aatgggcatg tgccatgact ttacctagaa
     7621 ttctaagatt ggaagatggc aaactaagac aattccctgt taaaaaaggc caatatcaaa
     7681 tccaaataga taaagattgt cattaccact taggaaatga tacagattat cttgaatttg
     7741 gttatgacag taatgcgcag caagtttaca ttgatcgtag ccatcttatt caaaaaattc
     7801 taggtgaaga agaacaggac actagtcgac ggtatgtaga tattgaagct aaagaattgg
     7861 aagttgttct agataaaaat tccatcgaga tttttgtcaa tcaaggtgaa gcaagcttga
     7921 ctgcaactta ttacttaacg gtgccagctg agctatcacg aattgattaa aaattaagtt
     7981 atttctccta aagaaaaagt tctctttcta aaatagtgga aagaggactt tttgtgtttt
     8041 gggtatataa gcttagttta tggtatttgt aaaattggtg ttggattatg atttaaacta
     8101 gttttctaaa gaatttgaaa aaaattttat ttaagcaaaa aaaccttggt tccaaggctt
     8161 ttcctgttg
//
LOCUS       DAVKJU010000078         7958 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVKJU010000078 DAVKJU010000000
VERSION     DAVKJU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.078"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(1..84)
                     /locus_tag="VW191_001820"
                     /pseudo
     CDS             complement(1..84)
                     /locus_tag="VW191_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(130..363)
                     /locus_tag="VW191_001821"
     CDS             complement(130..363)
                     /locus_tag="VW191_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU9624547.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(369..1361)
                     /gene="plsX"
                     /locus_tag="VW191_001822"
     CDS             complement(369..1361)
                     /gene="plsX"
                     /locus_tag="VW191_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU9624548.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIVGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1358..2128)
                     /gene="recO"
                     /locus_tag="VW191_001823"
     CDS             complement(1358..2128)
                     /gene="recO"
                     /locus_tag="VW191_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU9624549.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2125..3294)
                     /locus_tag="VW191_001824"
     CDS             complement(2125..3294)
                     /locus_tag="VW191_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU9624550.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAVFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3443..4453
                     /locus_tag="VW191_001825"
     CDS             3443..4453
                     /locus_tag="VW191_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEU9624551.1"
                     /translation="MSFLSKNGAGILVCLLISIVSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(4566..4646)
                     /locus_tag="VW191_001826"
                     /pseudo
     CDS             complement(4566..4646)
                     /locus_tag="VW191_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4681..5118)
                     /locus_tag="VW191_001827"
     CDS             complement(4681..5118)
                     /locus_tag="VW191_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU9624552.1"
                     /translation="MSQEFINSSDGVIRQYLVTSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5203..7836)
                     /gene="polA"
                     /locus_tag="VW191_001828"
     CDS             complement(5203..7836)
                     /gene="polA"
                     /locus_tag="VW191_001828"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU9624553.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNMSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDQLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2203 a         2045 c         1462 g         2248 t
ORIGIN      
        1 tggagtttgt gcttgaggat atatcttcat gagcccttga taaccactgt cagccaagat
       61 tttaccagct tgtccgatat ttctcatttc taaaaaccat ctacttccgt tgacttggaa
      121 ctccgattgc tattttcctt gaatgatttt aaccacatct cctacgtttt ggagttggtc
      181 aatttcttca tcgctgattt cgatactaaa ttcatcttcc agagtcaaga taaactccat
      241 caagtcaaca gaatccgcat ccaaatcgtc tttcagactc aaggattctg tcacgacaaa
      301 gtcctctccc tgtcgctctt ggataatggt cacaatacgg tcaaaaattt ctttttctgt
      361 catctctttt attctcctga aaattcacgc gcagtctggg caaccacgtc tgtttctagc
      421 atggtacgga tctgacgaat cgtactataa acagccttgg catcgcttga gccatgagtc
      481 ttgacaacag gtgccttaac accaaacaag accgctccac caacatctga ataattgagc
      541 tgttttttca aaccactgag gctgtccttg agaaggaggg cacctagttt cgctcgaaga
      601 ccaccacctg taatagctgt cttgagcaag cccacgattc ccatagctgt cccttcgatg
      661 gatttgagca cagcgtttcc cgtgaaacca tctgccacaa caacatctgc aacgccattc
      721 atcaaatcac gcgcttccac gtttccgata aagttcaaac tttcatcagc cgccagtaat
      781 tcataagttt ccttacgaag cgggtcgccc ttgctactct ctgttccgtt gttgagcaaa
      841 ccaacgcgtg gttgcgcaat gccacggaca tttttagcat agaaggaacc tagaaccgca
      901 tattgatgga ggtgctgggc tgtattttct gcattggcac caaggtctag catgtcaaaa
      961 ccttttccat caacggtagg caaggtagac atgagtccag gacggtcgat attcttgata
     1021 cgacccacga tgaagaatcc tgctgccaac aaggcacctg tattcccagc cgaaaggaca
     1081 gcgtctgctt caccatcttt gacagccttg gctgccaata ccatactggc atttttctta
     1141 ttcctaatag ctctcgtagg ttcatcatcc gaatcaatct tctcatccgt atggataatg
     1201 ctgacgcgct ctgtcgctgt cagatattgc ttgattttag cttcatctcc gtaaagttga
     1261 acctcgatat ctgaaaagtc agatagggct tgattgacac cctcaacaat ggcctgaggt
     1321 gcgtaatcgc cccccatggc atctactgcg atttttttca tttcttttcc tcttttagta
     1381 attgtcccca gtctgctagg gaatcaataa atttctttga ttttaggtga atcccaacgt
     1441 actcttcata taattgatcc ataaattggc gtagctcttg cttgattcca ggcttgagcg
     1501 aaatggtctc caaagtctca aaatcaatag cttgaaattg attgagcaga taggggatat
     1561 tgggattgag atgacaacgt ctctcatcct catgataatg ctctggacag aggcaggctc
     1621 catatttgaa agaaaagtca aaagcctgac caacccgatg gcagaagaca cactcattaa
     1681 aattgaggct gattccaaat cgagtcaaaa tttgaatttc aaaaatattg gtcaaaacct
     1741 gataatccaa gcctgcttcc atcaactcca aagtcttttg caaaaaagca aacaagggag
     1801 catcctgctg attgtcctgc aaactagcat ctgcaagagc tgccacatag gtcgcatagg
     1861 ccatgacaaa gaggtcacta ttaatcttgg gaaaagtcat gacctcatga tagtcttcga
     1921 tgtaactgag tccgtcatca ttgattcgca agagaaatcg tgccaacacc aagggctgaa
     1981 taacaggcgc cagcttagac tgaccagcgt gtttgacaaa aaacatgcgt ttgccaacct
     2041 gctctgtaaa aattttgacg agcttgtcat cctcacgaaa attgcgattg taaagcacca
     2101 agccttgact cgtgatagac tgaatcatgc ttctctcatg tactcctcaa gtcgtttcat
     2161 ggcttctttg atagtctcca tgctggctgc ataagatagg cggacgtagc cttccccgta
     2221 acgtccaaag gctgcaccag ggataaaggc aacggccttc ttctgagcaa aatccttcag
     2281 aaaagcaaag gagtcttgat tgtagcccgc tggaatttta gcaaaaatat agaaggcacc
     2341 gtctggtttg ataatctcaa aaccaagagc agtcattttt tcgatgatat agtcccgacg
     2401 ctggatatat tccttcttca tgggctccgc atcattttta ccagccgtca aggcttctac
     2461 cgcagcatgt tgcgccatgg tatttgcggc agtgaccaag tattggtgac tcttgattaa
     2521 ctgggctgtg aagaccgcag gagcgaaaat cagccccaaa cgccaacctg tcatggcatg
     2581 cgatttagac aaaccattga taataatagc ctggtctctc aacatcgttc ctagagacac
     2641 atgggcttcg cctgtgtagg tcaattctga gtaaacctca tcacagacaa caaaaatttc
     2701 gtacttgcgt aaaacagctg ccaaggcctc taactgctct cgactgtagg taattcctgt
     2761 cggattggct ggatagttga gaataaccgc cttgagctta tcaccctgct ccaaaatggc
     2821 cttctccaac atctcaggag tcaagacaaa accattttca gtcgtatcaa tctcaacaat
     2881 ttctgcccca actaagttaa caatcggttc atagcctgga taagcaggag ctggtaaaag
     2941 taccttgtct ccctcttcca aaatagccgt caaagtcgca gataaagcct ctgtcgcccc
     3001 aattgtaacc aagatttcat tttcaggagc atagtccagt tggtactttt ccttaacaaa
     3061 gtcactggct gcctgacgta gagtcagcag accactcatc cctgtatagt aggattggtt
     3121 ctgatcaatc gctcgcttgg ccgcctcctt gacatggtct ggcgttgtaa aatcaggttc
     3181 ccccaaggtc aaacgcaaga ccccaggaat ctccgaaata acctggtcaa actgacgaat
     3241 caacgaaact tgaattttat ctaactgttt attaaagcgc ttagttaagt ccatagatac
     3301 ctcctacttt caaaacgtat ctctattata ctacaaaagc cccccgaccg caaaaataca
     3361 ttagagaata gctttccact ttctatttta cttttcctgt ttacaggtct ataatggtaa
     3421 cagattctat ttggaggttt ttatgtcatt tctatcaaaa aatggagcag gcatcttggt
     3481 ctgccttctc atttctatcg tatcttggta cttaggagga ttcttccctg ttattggcgc
     3541 acccgttttt gcgattttta tgggcatgct cctacatccc tttctctcgt cttataaaca
     3601 actggatgtt ggattgacct ttagttccaa gaaattgctc cagtatgccg ttatcttact
     3661 tggttttggt ctcaatatct ctcaagtctt cgcagttggg caatcttcac tccctgtcat
     3721 cctttccacc atttcaatag ccttgattgt tgcctacctc ttccagcgct tctttgccct
     3781 ggatacaaaa ctggctacct tgattggagt aggttcttct atctgtgggg gttctgccat
     3841 tgcagcgaca gcgcctgtta tccatgctaa ggaaaaggaa gtagcccaag ccatctccgt
     3901 tatctttttc ttcaatgtct tggctgcgct catctttcca accctcggca cctggcttca
     3961 tctatccaat gacggcttcg ccctctttgc aggaactgcg gtcaatgata cttcctctgt
     4021 aaccgccaca gctagcgctt gggacagtct ctaccagacc aataccctcg aatccgcaac
     4081 cattgtcaaa ctcacgcgca ccctagctat catccccatc actctctttc tctcctactg
     4141 gcaaagtcgc gagcaaaaaa ataagcaagg cttacaactg aaaaaagtct tcccactttt
     4201 tatcctttac ttcatccttg catcgctcct aaccaccctt ctgacctctc tcggtgtgtc
     4261 tagtagtttc tttacccctc tcaaacaact ctctaaattc ctcattatca tggccatgag
     4321 tgccatcggt ctcaaaacta atctggtcgc tatggtcaaa tccagtggaa aatccattgt
     4381 tctcggagcc gtctgctgga ttgccatcat cctcaccagc cttggtatgc agacccttat
     4441 cggcattttc taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta
     4501 tatatgttac tgacttcgtc agttctatct gcaacctcaa aacggtgttt tgagctgact
     4561 tcgtcagtcg tatctacaac ctcaaaacag tgttttgagc agcccgcggc tagtttccta
     4621 gtttgctctt tgattttcat tgagtataac acaaaaggta gcccatcagc tacctttttc
     4681 ttatgcttcc tcaatcaagc gagtatgttc tctcttgata cagcgattca tcacgatatc
     4741 atcacatcca ccatcacgca aaatctcttt cgcttctaaa ctttcaagtc ctagctgtgc
     4801 ccaaaaaatc ttggcatcag ctttgagaaa atcacgcgcc acatcgggca gaaattcact
     4861 gcgacgataa acattgacaa tatctacagg aaaaggaatt tcagcgaggc tagcataagc
     4921 cttttcaccc aagatttcgc cacctgccgc cttgggattg actgggatga ttttatagcc
     4981 ccgagcctgc atttcctttg tcactcgatt gctggttgtt tcttcacggt cagacaaacc
     5041 caccacagca agggttttac tcgttacgag atactgacgt atcacgccat cacttgaatt
     5101 gataaattct tgactcatag aaatcctcct ttttcatcag tatagcacat tttgaaaagg
     5161 tttgcagaat tctactacaa aaaaggagga ctagccccct ttttatttag cctcgtacca
     5221 ggttgctcct tcattctcat ctgcgataag aggaacactg agttgaatgg cttcttccat
     5281 ggtttgtttc accaattttt tcatctctac caattcagat ttaggcactt caaggacgat
     5341 ttcatcgtgc acttgtaaca gcatcttagt ctgataacca cctgcaacca aggctttatc
     5401 cagctgaatc atggcaatct tgagaatatc tgctgccgaa ccctggatag gtgagttgat
     5461 agcagttcgc tccgcaaaac cacgaatatt gaagttgcgc gaattgatat ctggcaactc
     5521 acggcgacgc ttaaagaggg tctctacata gcccttatca cgcgcctccc gcaccacttc
     5581 atccatgtag tttttaatac ctggaaaacg ttcaaagtag gtatcaatgt aggctttggc
     5641 ttccttacga ctaattccca aattattaga caagccaaag tctgaaatcc cataaaccac
     5701 tccaaagtta actgccttgg cattgcgacg gtcatttgca gtcacattat caggacgctc
     5761 aatgccaaag acccgcatgg ctgtcgaagt atggatatct gccccctctt ggaaggcctt
     5821 aatcaagtgc tcatccttag aaatatgcgc caaaacgcgc aattcaatct gtgaatagtc
     5881 agagctgagt agcacactat cctcccactc tggcacaaaa gccttccgaa tcaagcgccc
     5941 ttgttccaat cgggcaggaa tattttgcaa gtttggatcc acactagaca aacgcccggt
     6001 ctgggtcaaa tcctgcatat agcgagtatg aatctttcca tcagccaaaa tccagtcctg
     6061 caagccaatt acataagtag attgaatctt agcaatttga cggtaatcca ggattttctt
     6121 aacaatcgga gcaataggag cgagacgctc caacacatcc actgctgtcg aataacctgt
     6181 cttggttttc ttagtgtatt ctagaggaag tcccaatttc tcaaagagaa gcacgcccaa
     6241 ctgcttaggc gagttgacat taaactcctc accagccagc tcgtaaatct cttgagtcag
     6301 tttttcaatg acaagctcat tttcagcctg catctcaagc aaggtctctt tcttgaccac
     6361 aatcccagca atttccatct tggcaaggac aaaagccaga ggttgctcca tatcataaag
     6421 aagctctaat tgcccatttt cgctaagttt ttcaagtaaa ataggctctg tttctaccaa
     6481 aacagcaagt ttacaagcta agtgttccaa gaatttctca cgttcaggaa tggccttttt
     6541 aacaccctta ccgtagaaag tttcatcatc aaccaagtaa gtctgaccat aaagactagc
     6601 gatggtcgca atttcattgt cctccacagt cgaaaggagg tatttagcca aacggatgtc
     6661 aaaagcaggc gcctgcaaat ctacaccaaa acgttgcaaa agaactttaa ccttcttaaa
     6721 gtcataaact ctcagagatg ttttttctaa gaaatccttg aaaatcgggt cttgcaacag
     6781 ctcaagcttg tctgtggcat agagctgatc cccacaagac caggcaaatc caaccaaatt
     6841 atctgtatgg taattctcac caaaaagctc aaagtggaag atagactctt cactcagcat
     6901 atcttgactg atttggtcaa caatagtaaa atccaaaccc tcagccacat cagctgacga
     6961 catatttaaa gcctgcttga gctgtttgaa gcccatctca tcgtagaatt tcccaagatt
     7021 ttcaacatct ggaccactat agaccaagtc ctctaaacca atcgcaatcg gtgccttggt
     7081 atcaatggtc gctagtgttt tagacaaaaa ggcctgttcc ttgtcattga tgagattttc
     7141 cttcatctta gaagtcttca ttccatcaat attttcataa atcccctcaa gcgaaccatg
     7201 ctccagcaag agcttaatac ccgtcttttc accgactttg gtcaccccag ggatattatc
     7261 cgacttatca cccatgagcg ccttgagatc gataaactga gctggtgtga ggcccatttc
     7321 ttccatgagg taatctggcg taaaggcctc aaactcagcc acacctttct tggaaatttc
     7381 aaccaccgta tgctcatccg tcagctgaat caaatccttg tccccactga caatggtaat
     7441 atcaaaacca tcctgctctg ctagcttatc cagcgtccca atgatgtcat ccgcctcata
     7501 ctgagccagc tcatagtgac gaatccccat atgatccagc aactcacgaa tgaaaggaaa
     7561 ttgctcacga aactcatcag gagtcttggc ccgaccaccc ttatagtccg catacatctc
     7621 tgtccggaag gtcgtctttc ccgcatcaaa agccaccaaa atatgactcg gctcaacccg
     7681 ctccaacaaa tgactcaaca tcaactgaaa accataaatc gcattggtat gcaaaccagc
     7741 cgcattctta aaacggtcca actgctgata cagcgcaaaa aacgcccgaa aagctacaga
     7801 agacccatca atcaataata attttttctt atccatacac ccattataaa ggaaagaatc
     7861 aaaaaatacc attgggaaga gctagagcaa gtatttttca aactttttcc gaataaatag
     7921 atagagccag agaatttagt aaacctagat ttaaaaat
//
LOCUS       DAVKJU010000079         7441 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVKJU010000079 DAVKJU010000000
VERSION     DAVKJU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7441
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.079"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(176..1591)
                     /locus_tag="VW191_001829"
     CDS             complement(176..1591)
                     /locus_tag="VW191_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9624554.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGSGTFNRY
                     KKMSFTWYKEVIESNGESLFK"
     gene            complement(1604..3442)
                     /locus_tag="VW191_001830"
     CDS             complement(1604..3442)
                     /locus_tag="VW191_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEU9624555.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVNQGYKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFFAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEVGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3460..4299)
                     /locus_tag="VW191_001831"
     CDS             complement(3460..4299)
                     /locus_tag="VW191_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEU9624556.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(4599..7187)
                     /locus_tag="VW191_001832"
                     /pseudo
     CDS             complement(4599..7187)
                     /locus_tag="VW191_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT         2462 a         1541 c         1021 g         2417 t
ORIGIN      
        1 ataaccaatc tatcctaagt taactattta taatcggctc tctgtaacaa aattaaatta
       61 attaatcccc ctataaaata aagaggttta gaatttgatg tcttccaaac ttctttattc
      121 catatttaat gaaatgccac cttaaccgtg ataatagcta gtcatcaata aaaaactatt
      181 tgaataagga ttctccattt gattcaatca cttctttata ccaagtaaaa gacattttct
      241 tatatcgatt gaatgtacca cttccatcat cgtttcgatc aacataaatg agaccgtacc
      301 ttttagaaag ttgtgcagtg gacatagaaa cacagtcaat acatccccaa gacgtatagc
      361 ccataatttc aacaccatcc tgtagagctt cagcaacttg caataaatgt tctttcatat
      421 actgaattct ataatcatct tggacggtta agttattaag ttcatctttt attagttgat
      481 ctttagcacc taatccattt tctactataa ataatgggat ttgataacgg tcataatatc
      541 tatttaaaat tatacgtagt ccaattggat caatttgcca tccccactca gaagactcta
      601 aataaggatt tactaagcca ccaataatat tcccttctcc tgaattatac tgtgttggaa
      661 gagcagattg agtcacactc atgtaatagc taaaggataa aaaatctaca gtataatttt
      721 ttaataactc tgcatcttca gctgcaaact ctatgttaat gtcatgttcc ttaaaatatc
      781 tttttgcata attcggataa taacctctaa catgcacatc tgaaaataga taatttagat
      841 tctcatactc atgagtcgcc catacatctt ttggatttgg agtcattgga taagctggca
      901 tagctaatac catacacccc accttaaact ctgaattaat ctcacgagca atttttgtaa
      961 ccaaactcga ggcgactaat tcatgatgta tagcttgata taattcttgt ttcgaaagat
     1021 tctccttagg tatatctatt cctccactag taaacggtaa ttccaaaaca gagtttactt
     1081 cgttaaatgt aagccaatat ttaactttat ctttatacct ttctaaaact gttcgagcaa
     1141 atttttcata aaaatgaatc attctcctat caacccatcc atgatatttt cttgctaaat
     1201 ataatggagt ctcatagtgt gaaagagtta caagtggctc tatcccgtga gcatgtagtt
     1261 catcaaacaa ttcatcataa tatttcaaac cagcttcgtt aggttcttct tcatctcctt
     1321 ttggaaaaat tctactccat gcaatagaag tacgaaaaac attaaagccc atttcagaaa
     1381 acaaggatat atcttcctta tatttatgat aaaaatcaat acctaacaat tttaagttat
     1441 cttctgtagg attttctgtt gcttctccta atccaccttt gggtaacaca tcctgaactg
     1501 ataatccctt accatcttca ttatatgctc cctctacttg attagctgca acagctccac
     1561 cccaaagaaa atcatctgga aaaatggtca taactttcct ccattataat attaccagta
     1621 attccttaga atgctcgatt gtctgattat taggtaatac taatacatct agaaaatcat
     1681 tggtattcgt tacaattact ggtgtaactg tttcgtagcc tttagtcttg attaaattca
     1741 agtccatttc aaaaatcaac tgatttttga aaactctgtc tccttcttct acatgactaa
     1801 taaaaccttg accttttagc tcaacagtat ctaatccaat atgaattagt aactcaacac
     1861 cctcatcact cttcaatcca attgcgtgct tagtcggaaa aatatttgta attttcccgt
     1921 caaatggtgc ataaacctta ccttcacttg ggataatcgc tactccgtct ccaattaatt
     1981 tatctgaaaa tgttttatcc tggacatcgc ttaacggaat gatttctcct gatataggag
     2041 aaaatatcat ttttttattt gaaactccaa cttcaacttc taaattgcta gaactctctt
     2101 cttcatcgat tccaaatata taagctaata caaaggtaat aacaaccgaa atgaccgcca
     2161 caattaaagc atttacaata tttgatggca catcagaata aataaattga ggcaacgcta
     2221 tcaaagatgg gacagcaaat agatatgctt taacactagt aagacctgca aataatcccg
     2281 ctaatccacc accaatcata gctgcataaa gcggtttttt atattttaaa gtcacaccat
     2341 ataatgcagg ttcggtaatt cctgcaagta aggctgagaa acctgctgca aaagcaattt
     2401 gttttgtatt attattttta ctctttaatg caacagccat cgaagcagcc ccttgagcta
     2461 agtttgaccc taacattgct ggaagaatta atacgtctgg agtagcaata gatgccgcaa
     2521 aaaaaatagg tgcaaaagcc caatgcattc cagtcataac aataaatggc ataatagcac
     2581 caagaatagc taatgtaagc catccagcta caccatacat ttgcccaact agatttgata
     2641 atccttcacc aacaattact ccaataggtc cgactacaac taaggcaata cagcttgata
     2701 ctaataatac tagcgtaggt tgcaaaaaac tcttagtaac agctggtgtt aatttagcaa
     2761 ttattttttc aatatatttc atcaaccaaa ccataataag aattggaacg actgatgaac
     2821 cataactagc tggtgtcaca ggtgcaccaa ataaactaag aggattccct gattgcacca
     2881 tttgaacaaa atttggatgg agaagtacac ctgctacaga catagctaat gtagatgtta
     2941 cttttaattt ttgtgatgca gaataagcta ataacagcgg taagaaataa tatggagcat
     3001 ccccaaaaaa tgtcaaaaaa gcaatagtct gagaatctga ttgcaatata ccaagcattg
     3061 gtaaaatgat taccaagact ttcaacatac ctccccctaa cattgctgga atgattggag
     3121 tcatggaacc agcgatatac tcaatgattc tttctaaaat attccctttg tacccttgat
     3181 taactgaatc ggattcaaaa ttgccaagtt taacgaattc tttataataa ttagctacat
     3241 cattaccaag tataatttga tattgtccat tctttttcat aatacctatt acacctggta
     3301 tcttcttcac atcatcatca ttgactaaat tttcatcttt taattctaat cttaaacgtg
     3361 ttacacaatg ggtaactcta ttgacatttt tttcgcctcc aattacatcg aggatttttt
     3421 gtaccgtatc tttataactc atggtattct cctattctat taatctaaat tttttgttaa
     3481 gcgacgaata tgagccatca aataaactaa ttcactagaa gtcagcaaat aattgtactc
     3541 cgtttgtata aacattgcca cctgttcacc acattcatat tctctaggat atttattttt
     3601 cattaatgct aacaagtctt catcatcatc gtcacaataa gttgtatttt caaccaagcg
     3661 atgggcaaat aattttatat gagttatgaa tctataataa tcaatagaat cttggttaaa
     3721 ctcagtacaa tagaaatctt gaatgatttt ctcaattccc ataattattt cagtgatttt
     3781 atacgactct tgaaaattat tttctaaatt agcattaaca aaatgtagcg ctatgaatgc
     3841 agcttcgtca attggaagtt caatacctaa ctcttttttt attatttgta aagccctttt
     3901 cccaagttca aattcttctt tataatattt cgatatttcc cagcgtaggg gattcgaaat
     3961 aataatccct tctttataac gttctatgct cgaagaaata tgatccgtta aattaatata
     4021 gataatttca tcaaaattat tacccaatgt tattttacct aggttaatta ttttttcggt
     4081 gatgtacacc aactccaaag gaacgttaat aaacatctct gtaaaacggg tcatattatc
     4141 tctatttttg agaaaaaaac ttttctcaat atcaattgga tttactttat ctccagtttt
     4201 tcttccaaaa gctattcccc ttccaaaaag aagaatatcg atatcttttt tactttgcgc
     4261 aattacggca ttgtggttta gtattcgttt tatgatcata aaatttccct tttaaaataa
     4321 aaggaaccta cttaaaaaca tcaagaaact tcttctgatg tgattaaata ggttcctgct
     4381 cacttgtatt agtgataaca tccttttatt tcaattttta atggtgacat gaagcttaaa
     4441 taattaataa ctctgtaacc attttcattt tatgatattt caatctattt gtcaagtact
     4501 tataaaaatt tttttatttt gtgtgattcg aatgtgataa tggcaacagt ctcactaaag
     4561 aaatgaaaat catacataga aatttttaaa aattttctct atcgtaaacc tgccactagt
     4621 tcagaaacta tactagctga ttccaaacgt aatatggtca tctcatctac cttccctaat
     4681 aatggcattg caccatacca aacaatagtc ttattcacca aaagaatagg cgttactatt
     4741 tttgattgag taaaagacac tttaattttc tggctcttca actggtctag ccatgcttta
     4801 tttgcaaccg tctctggtat acatatctca atttgactaa tcctaaattc ttttagaaaa
     4861 ttcatcagtt ttgtctggtg cacataaggt aaaataagca gacattcctg tctttcccct
     4921 gctaagtcct ctctcaacac cttctcatat ctactatcaa cataaacgaa ttgtttctcc
     4981 tcaccctcga tgacacgata atccatcttt cgataagcta cttgtcgttt ctgaaacatc
     5041 ttttctaaat aaggaacatg aatatccaca taatcgaaaa tacgcaccaa agacttatcc
     5101 ttgtagtttc tatgaatccg acctgcatac tgaattaaat tatttttcca agaaaagggt
     5161 gctgccaaga taagcgtgtc caactgaggt aagtcaaaac cttcgccaat gtattttcca
     5221 gtagacaaca aaacaaaccc tttatctaac tgttctaacg tctccagtaa actcgttctt
     5281 tctcggactt tggtttttcc gctaataatg taacagtcat caacctcttt ctctttcaat
     5341 aacttttcaa agacatctat ctgttgaatt cgattaacta aaaccaagat attccgtcct
     5401 tctgccactt gggctagaat atccttgaga atcaactgat tcctcgctga gtctgtagca
     5461 atccaatcac tgggctgtat aaaattactt gctttggtct tttcaatttc tctgaaaaac
     5521 aataggttca tgaccattct tacgctcagg cgtagccgtc aaaccgtaaa gatacttccc
     5581 tctaaactga gcaacaactt tttcaaacat caaggcagag atatgatgac actcatccac
     5641 aatcatcatc tcatactcat ccaaaagact ttgactattt tccaacttaa atagagattg
     5701 aatcataaca acatcaacca gtttactcag tcatttctta gccccaccgt actgcccgac
     5761 atagcctatt accctttcac gacctgatgg tgtataacgg gtagcttctg cctcttcgaa
     5821 agtcaaaaaa cactttaaac gatccagtca ttggtctaag agttgcctat tatgaactag
     5881 gataatggtt tttgtcttac gttcagagat aagagcagca cctaaaacgc tctttccaaa
     5941 acctgtcccg catgaagtaa accattttct ttagaagtca tatctgacag ggctaactct
     6001 tgctcaaaag tgagttctcc cttaaatgct actctaatag acctttgtac ttttctccta
     6061 tcctccacaa ctacctgctt aaatttatct tgcaatggat ataacaagcc tcttggcagc
     6121 cataaataat aatcggattc tccaaataaa tacattcgct caggaatttg atagtttggc
     6181 tgtcgcatag cctgctttaa ataaaattcg ggattagaaa atgaagccat atttttcaag
     6241 aaaaacagtg tcttgggtgt cacagacgat tttttaagtt ggatcatatt ggataaaacg
     6301 accttcaact ccttatccaa ttcttgcttg cctaactcct cttggattaa cagtgccact
     6361 ttagcagttg aaatcttctg aattccttgc agatacctcc attggtctcc ataaggttga
     6421 aaatgttcat ccacaaggac cgttcgccct tgatgataag cttctccttg aaaaggaaag
     6481 gaaattaaat taccaaatcc acctttagga agaacatcct gatttggaaa catgcgatca
     6541 aaagaatcaa aggacagttg catactttcc tgcatagcca attctaatag tttctttcca
     6601 aacagacgag cctttcgact cagaatcgct tcctcaaaga agaaccaaat atggagtccg
     6661 taacccgaac gagaaatctc taaatgggct tccatctggc gttccctggc tattcttcga
     6721 atggttaaac cagcttcttt ccaatctcct tcatcaaaat ccagtaccaa aaaatgacag
     6781 ctatcatcta agtgcatagg aaagataccg atagctgtct ccccacgaaa atgagatttc
     6841 aaaacggaat ccgtcaatgt ctggaaactc cgtttttcag gtagcaactg cttccaacca
     6901 taatcatagg aaggaaaata ctgaattttc tcttgatcat tgataaaact cttagcataa
     6961 acatcataac ggccagcaaa cacagaggta aacaattgta atttttcttg cgtcgtcaag
     7021 cgactacttt gaagttggaa catctcctcg aactgagagc gttccataac aaactgactc
     7081 ttgtctttaa gacgtttaca caagaaaaac tcactatccc catcaaaaga agaaaagaca
     7141 tcaagtactt ctaccacaat tccatccaaa gacacaaaaa cagccataag agtcacctcc
     7201 ttgattccta taggctgatt ataacaagaa tagctgaaat tgtacacgaa aataaaatcc
     7261 gaatagcact cattttgtat gcgactaata gtccatctcg ctccggaatg tacgaggtaa
     7321 atagagttta aacgagcagt ctataaaatt caaaaaacgc atattaccaa tgttgttttg
     7381 aactacaccc caaaagttag acagaaaaaa tctaactttt tggggtgttt ttattatgaa
     7441 a
//
LOCUS       DAVKJU010000080         7334 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVKJU010000080 DAVKJU010000000
VERSION     DAVKJU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7334
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.080"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..706
                     /locus_tag="VW191_001833"
                     /pseudo
     CDS             1..706
                     /locus_tag="VW191_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            817..1743
                     /locus_tag="VW191_001834"
     CDS             817..1743
                     /locus_tag="VW191_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU9624557.1"
                     /translation="MDLSQTMPSYAQVVSLYDDTSISVSNNETDKVLAGSLYTDTNEQ
                     GLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQFQTKIVRVINDE
                     DDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTEELQKNKKYTVLS
                     QQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRSKEIGVMKAVGYQ
                     NRGVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSITKVFELNLLSVLG
                     TLVFALLLGYVSAYFPARKISKMDPVESLRYE"
     gene            1920..2927
                     /gene="gap"
                     /locus_tag="VW191_001835"
     CDS             1920..2927
                     /gene="gap"
                     /locus_tag="VW191_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU9624558.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2994..3830)
                     /locus_tag="VW191_001836"
     CDS             complement(2994..3830)
                     /locus_tag="VW191_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU9624559.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3956..6151
                     /locus_tag="VW191_001837"
     CDS             3956..6151
                     /locus_tag="VW191_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEU9624560.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            6204..6356
                     /gene="rpmG"
                     /locus_tag="VW191_001838"
     CDS             6204..6356
                     /gene="rpmG"
                     /locus_tag="VW191_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU9624561.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6366..6542
                     /gene="secE"
                     /locus_tag="VW191_001839"
     CDS             6366..6542
                     /gene="secE"
                     /locus_tag="VW191_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU9624562.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6597..7133
                     /gene="nusG"
                     /locus_tag="VW191_001840"
     CDS             6597..7133
                     /gene="nusG"
                     /locus_tag="VW191_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU9624563.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYALVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2307 a         1273 c         1581 g         2173 t
ORIGIN      
        1 ctaaaccacc aagtaaaagg aacaaaacca agaaaggttg atagagatag acttaaaaac
       61 tatcttactg acaatccaga cgcttatttg actgaaatag cttctgaatt tggctgtcat
      121 ccaactacca tccactatgc gctcaaagct atgggctaca ctcgaaaaaa agaaccacac
      181 ctactatgaa caagagccag aaaaagtagc cttatttctt aagaatttta atagtttaaa
      241 gcacctagca cctgtttaga ttgatgaaac aggattcgat acttattttt atcgagaata
      301 tggtcgctca ttaaaaggtc agttaagaag aggtaaagta tctggaagaa gatatcagag
      361 gatttctttg gttgcaggtc gaacaaatag tgagttaatc gctccaatga cttacgaaga
      421 gatgatgacg agcgactttt ttgaagcttg gtttcagaag tttctcttac caacattaac
      481 cacaccatcg gttattatta tggataatgc aagattccat agaatgggta agttagaact
      541 cttgtgtgaa gagtttgggc ataaactttt acctcttcct ccctactcac ctgagtacaa
      601 tcctattgag aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat taccaagttg
      661 caataccttt tatgaggctc ttttgtcctg ctcttgtttc aattgactat agttcacgga
      721 tacagttggg aaagaagcta ttaaatattt ggagagtcct tttgggatga acatgtcaaa
      781 tattactata gatgggcaag aaaagatggg caagaaatgg atctgagtca aacgatgcct
      841 tcttatgctc aagtagtgag tctttatgat gatacaagta tctctgttag taataatgag
      901 acggacaaag tgttggctgg ctccctctat actgatacaa atgaacaggg attaacgatt
      961 ccaagcagtt tactaaaaaa ttggaatgaa cagacaggaa aaaatttgac agctaatgat
     1021 cttattggca aatcagtctc agccagcatt gtagaaagtg ctgccgaaac tagtaagatt
     1081 gctcaatttc aaacgaagat tgtacgtgta atcaatgatg aagatgacat ggaggacagc
     1141 aacagtttca tgctgtctca tcaaatggaa acgattttga aagaggctgg atttacgaaa
     1201 gctgtatctt attttatctt ggaactcaaa gatccatcac agacaaaagt agtaacagaa
     1261 gaattacaga aaaataagaa gtatactgtg ctttctcaac agagagttct tgatattgtg
     1321 attaccttta ttcgtgttat tcagggatta ttgattgtgc tttcatcaca agctattgtg
     1381 gtagcagcgg ttatgattgg tatcattatt tacatcaata tcatgcaacg ttccaaggaa
     1441 ataggtgtca tgaaagcagt tggttatcag aatcgtggtg tcaaaggaat ttttatttac
     1501 gaggctatct ggattgtagg catcgccttg ctgctggcat ttttggttgc acaaggggtg
     1561 ggaagtttgg cgaatgcgat tgtaagtcac ttttacccat ccatcactaa ggtttttgaa
     1621 ttaaatcttt tatctgtttt aggaactcta gttttcgctc tattacttgg ttatgtctca
     1681 gcctacttcc cggcgcgtaa gattagtaaa atggatcctg tagaatcgtt acgctatgaa
     1741 taataaggga ttccttgatt tacagtctta aaaagaaact cacatagaaa aacaatggcc
     1801 atacttgcaa ttcaactcaa atagttgtat aataggtttg ttgaaaggtg atttgtagtg
     1861 attcaagtta ctcttttcac aataaaaatt tcatgatttt cataaggagg aaatcactaa
     1921 tggtagttaa agttggtatt aacggtttcg gacgtatcgg tcgtcttgct ttccgtcgta
     1981 tccaaaacgt agaaggtgtt gaagttacac gcatcaacga ccttacagat ccagttatgc
     2041 ttgcacactt gttgaaatac gacacaactc aaggtcgttt cgacggtact gttgaagtta
     2101 aagaaggtgg attcgaagtt aacggtaaat tcgttaaagt ttctgctgaa cgtgatccag
     2161 agcaaatcga ctgggctact gacggtgtag aaatcgttct tgaagctact ggtttctttg
     2221 ctaagaaaga agcagctgaa aaacacctta aaggtggagc taaaaaagtt gttatcactg
     2281 ctcctggtgg aaacgacgtt aaaacagttg tattcaacac taaccacgac gttcttgacg
     2341 gtactgaaac agttatctca ggtgcttcat gtactacaaa ctgcttggct ccaatggcta
     2401 aagctcttca agataacttt ggtgttgttg aaggattgat gactactatc cacgcttaca
     2461 ctggtgacca aatgatcctt gacggaccac accgtggtgg tgaccttcgc cgtgctcgcg
     2521 ctggtgctgc aaacatcgtt cctaactcaa ctggtgctgc taaagctatc ggtcttgtaa
     2581 tcccagaatt gaacggtaaa cttgacggat ctgcacaacg cgttccaact ccaactggat
     2641 cagttactga attggtagca gttcttgaaa agaacgttac tgttgatgaa gtgaacgcag
     2701 ctatgaaagc agcttcaaac gaatcatacg gttacacaga agatccaatc gtatcttcag
     2761 atatcgtagg tatgtcttac ggttcattgt ttgacgcaac tcaaactaaa gttcttgacg
     2821 ttgacggtaa acaattggtt aaagttgtat catggtacga caacgaaatg tcatacactg
     2881 cacaacttgt tcgtactctt gaatacttcg caaaaattgc taaataattc ttgagttgat
     2941 agaaagcaag gctttgtggt cttgcttttt atatggaaaa atggatgaca cgatcatcca
     3001 ttctttttta attctttttc aaatgtattt gaaagggtag tgaaagttag cttctctaaa
     3061 gtaagtgggt gggtaaagga aagtcggaag gcatgaagca taagccgact tgtctttgat
     3121 ttactattat agagagggtc gcccaggata ggaagattat gatgcgaaag gtgcacacga
     3181 atctgatggg ttcgccctgt ctttagcttg caacgagcca aggaagtctt gtttgagaat
     3241 tgctttaatc tgcttacatg cgtttcagca tattgcccat tttttgcatc aactattctt
     3301 tttctacgat catggcgatc acgtccaatt ttgtctctga aaacaagttc ttttctgttg
     3361 atatttccat caactagagc ccaatattct ctagaaatct cttttttctc caataagcga
     3421 ttgagaatgg gcaggataaa aggatttttg gcaaagagaa ctaagccact ggtttccatg
     3481 tccagacgat gaacgacata gcaggtttgg ccaacatagg tactgacatg gttaagaagg
     3541 gcaatttcgt ttggttgatt accatgcgtt ttcatcccct ctggtttgtt tacaataatc
     3601 aagtgttgat cttgataaac ttcctgcact aagtctgggt tgccccaagg gatcgtcttt
     3661 tgggaataat cttcctcgtc aaaagtcaac tggcaaacat ctccaggatt tacgatttcg
     3721 ttccagtgga cttcttcttg atttatcaaa atatgtttct tgattctcaa aaaatgacgg
     3781 atttttctag ggatgaggag ttgttcctca agtaattgct ttaccgtcat ttgaggtaga
     3841 gaggcgggta atgtaaatgt gaattgcata cagatattgt aacaaaaaaa gccctatttg
     3901 gataggaaat agctaaattc ttgtcttcct atgatgaaga tgataaaata aacgcatgaa
     3961 attagataaa ttatttgaga aatttctttc tctttttaaa aaagaaacaa gtgaactaga
     4021 ggactctgat tctactatct tacgtcgctc tcgtagtgat cgaaaaaaat tagcccaagt
     4081 aggtccgatt cgaaaattct ggcgtcgtta tcatctaaca aagattatcc ttatactagg
     4141 tttgagtgca ggattgctag ttggaatcta tttgtttgct gtagccaagt cgaccaatgt
     4201 caatgatttg caaaatgcct tgaaaactcg gactcttatt tttgaccgtg aagaaaaaga
     4261 ggctggtgcc ttgtctggtc aaaagggaac ctatgttgag ctgactgaca tcagtaaaaa
     4321 cttgcagaat gctgttattg cgacagaaga ccgttctttc tataaaaatg acgggattaa
     4381 ctatggccgt ttcttcttgg ctattgtcac tgctggacgt tcaggtggtg gctctaccat
     4441 tacccaacag ctggctaaaa acgcctattt atcgcaggat caaactgttg agagaaaagc
     4501 gaaagaattt ttccttgcct tagaattaag caaaaaatat agtaaggagc aaattctaac
     4561 catgtacctt aacaatgctt attttggaaa tggtgtgtgg ggtgtagaag atgcgagtaa
     4621 gaaatacttt ggagtttctg catcagaagt gagtctggat caagctgcga ctctggcagg
     4681 gatgctcaag gggccggaac tgtataatcc cttgaattct gtagaagatt ctactaatcg
     4741 gcgcgatact gtcttgcaga atatggttgc agcaggatat attgataaaa accaagaaac
     4801 cgaagctgct gaagttgata tgacttcgca attgcacgat aagtatgaag gaaaaatctc
     4861 agattaccgt tacccctctt attttgatgc ggtggttaat gaagctgttt ccaagtataa
     4921 tctaacagag gaagagattg tcaataatgg ctaccgcatt tacacagagc tggaccaaaa
     4981 ctaccaagca aatatgcaga ttgtttatga aaacatatcg ctatttccga gggcagagga
     5041 tggaacgttt gctcaatcag gaagtgtagc tctcgaaccg aaaacagggg gagttcgtgg
     5101 agttgtcggt caagttgctg acaatgataa aactggattc cggaatttca actatgcaac
     5161 ccaatcaaag cgtagtcctg gttctacaat taagccttta gttgtttata cgccagcagt
     5221 tgaagcaggc tgggctttga ataagcagtt ggataaccat accatgcagt atgacagcta
     5281 taaggttgat aactatgcag ggatcaaaac gagtcgagaa gttcctatgt atcaagcctt
     5341 ggcagaatcg cttaatctac ctgctgttgc cactgttaat gatttgggtg tcgacaaggc
     5401 ttttgaggca ggcgaaaaat tcggactcaa catggaaaag gttgaccgtg ttcttggtgt
     5461 cgccttggga agcggtgttg aaaccaaccc tcttcaaatg gctcaagcat acgccgcctt
     5521 tgcaaatgaa ggtttaatgc ctgaagctca ttttattagt agaattgaaa atgctagtgg
     5581 acaagttatt gcgagtcata aaaattcaca aaaacgggtg attgataagt ctgtagctga
     5641 caagatgacc agtatgatgt tggggacttt caccaacggt accggtatta gttcatcgcc
     5701 tgcagactat attatggcag ggaaaactgg aacaactgaa gcagttttta atccggagta
     5761 cacaagtgac cagtgggtaa ttggttatac tccggatgta gtgattagcc actggcttgg
     5821 ctttccgacc actgatgaaa accactatct agctggctct acttcaaacg gtgcagctca
     5881 tgtctttaga aacattgcca ataccatttt accttatacg tcaggaagta cctttacggt
     5941 tgaaaatgct tataagcaaa atggaattgc accagccaat acaaaaagac aagtacaaac
     6001 caatgataat agccagacag atgataattt gtctgatatt cgagggcgtg cgcaaagtct
     6061 agtagatgag gctagccggg ctatctcaga tgcgaagatt aaggaaaagg ctcaaacaat
     6121 atgggattcg atagtcaatc tatttcgcta agatgcttgt caaagcctag ctttcttgtt
     6181 ataatggata agatggaggc gttatggcac taaaaaaagc aagcctagct tgtgcggttt
     6241 gtggttcgag aaactattca atcaagatca gcggaaaccc caagcctaca cgactagaag
     6301 taaataaatt ttgtaagcat tgtggcaagt acactacaca tagagaaacg agataggaga
     6361 gagcgatgcg ttttattgga gatattttta gacttcttaa agacacaacg tggccaactc
     6421 gcaaggaaag ctggagagat tttcgttcta tcatggaata cacagctttc tttgtagtaa
     6481 ttatttacat ttttgaccag ttgattgttt caggtttgat tcgatttatt aacatttttt
     6541 agaagattag tggagttaat tacactagaa atcttctatt tatgaaagga aatatcatgg
     6601 atagttttga taaaggatgg tttgttttac aaacttattc tggttatgaa aataaggtga
     6661 aagaaaatct attacaacgt gcacaaacct acaatatgtt ggataatatt ctacgcgttg
     6721 aaattccaac acaaacagtg caagttgaaa aaaatggaaa gagaaaagaa gtagaagaaa
     6781 atcgctttcc aggttatgct cttgtagaaa tggtcatgac agatgaagct tggtttgttg
     6841 ttcgaaacac accaaatgtt acaggatttg tcggatcaca cgggaacaga tcaaaaccaa
     6901 ctccattatt ggaacaagaa attcgtgaca ttttggtatc tatgggacaa actgttcaag
     6961 aatttgattt cgatgttgag attggtcaaa ctgtacgtat tattgatggt gcttttgcag
     7021 actacactgg taagattaca gaaattgata ataacaaagt gaaaatgatt atctctatgt
     7081 ttggtaatga cacagttgca gaagtaaacc taaaccaaat cgcagaatta taaccctgag
     7141 agaggctgga gcctctcttt tttgtgcagt ttaggagcta aagggaacag aatggagaaa
     7201 atggaacaaa tgtgttttct aatctgttag actgtatcta gaaaggggaa aattatgatt
     7261 aaagaattgt atgaagaagt ccaagggact gtttataagt gtagaaatga atattacctt
     7321 catttatggg aatt
//
LOCUS       DAVKJU010000081         7257 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVKJU010000081 DAVKJU010000000
VERSION     DAVKJU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.081"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(92..184)
                     /locus_tag="VW191_001841"
                     /pseudo
     CDS             complement(92..184)
                     /locus_tag="VW191_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            398..6832
                     /locus_tag="VW191_001842"
     CDS             398..6832
                     /locus_tag="VW191_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU9624564.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHNAIETSKDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKE
                     ENSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKGLSNLKNTKVLYT
                     YDRIFNGSAIETTPDNLDKIKQIEGISSIERAQKVQPMMNHARKEIGVEEAIDYLKSI
                     NAPFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDK
                     IPHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNA
                     QIFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRA
                     LRKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQ
                     TVEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLD
                     LRGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEG
                     TKSQVFSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNV
                     GDEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVI
                     NGKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIAL
                     QNTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYG
                     SISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDG
                     KQIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFES
                     VEEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDG
                     KPKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSS
                     GSKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVI
                     SREHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPAT
                     KIRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLI
                     TKDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTY
                     AGEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFADQADEK
                     GMISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEG
                     EASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIA
                     YDDGSALQYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIE
                     IKATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKE
                     TKAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKG
                     YALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRST
                     QAVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDK
                     SNVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNW
                     ELRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMD
                     GNVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKR
                     NNGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDT
                     GEVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKK
                     DAGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDL
                     QREDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIV
                     GIFLGLKKKNQD"
BASE COUNT         2838 a         1002 c         1389 g         2028 t
ORIGIN      
        1 cagttctatc cacaacctca aaacagtgtt ttgagctgac tacgtcagtt ctatccacaa
       61 cctcaaaaca gtgttttgag ctgacttcgt cagttccatc tacaacctca aaacagtgtt
      121 ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag tactaatctt
      181 attttttaac gtgtgctaaa aaataaatta atgcatgtta atttttttga cttaaatttt
      241 tttaaatgat atactgtttt tatagagaga taacaattat atataaatgt actactctat
      301 ttcctagata atacttagta aaacttttta taacaaaggc tagcaaagtt aaagtttatt
      361 atctattgga agttaaataa atatgtaagg aattaaaatg aaaaaaagca cagtattgtc
      421 attaactaca gctgcagtta ttttagcagc ctatgcccct aatgaggtag tcttagcaga
      481 cacatctagc tctgaagatg ctttaagcat ctctgataaa gaaaaagtag tagtagataa
      541 ggaaacagaa aataaagaga aacataaaga tattcataat gctatagaaa cttcaaagga
      601 tactgaagaa aagaaaacaa caattattga ggaaaaagaa gttgttagta aaaatcctgt
      661 aatagacact aaaactagca atgaagaagc aaaaatcaaa gaagaaaatt ccaatcaatc
      721 ccaaggagat catacggact catttgtgaa taaaaacaca gaaaatccca aaaaagaaga
      781 taaagttgtc tatattgctg aatttaaaga taaagaatct ggagaaaaag caatcaaggg
      841 actatcaaat cttaagaata caaaagtttt atatacttat gatagaattt ttaacggtag
      901 tgccatagaa acaactccag ataacttgga caaaattaaa caaatagaag gtatttcatc
      961 gattgaaagg gcacaaaaag tccaacccat gatgaatcat gccagaaagg aaattggagt
     1021 tgaggaagct attgattacc taaagtctat caatgctccg tttgggaaaa attttgatgg
     1081 tagaggtatg gtcatttcaa atatcgatac tggaacagat tatagacata aggctatgag
     1141 aatcgatgat gatgccaaag cctcaatgag atttaaaaaa gaagacttaa aaggaactga
     1201 taaaaattat tggttgagtg ataaaatccc tcatgcgttc aattattata atggtggcaa
     1261 aatcactgta gaaaaatatg atgatggaag ggattatttt gacccacatg ggatgcatat
     1321 tgcagggatt cttgctggaa atgatactga acaagatatc aaaaacttta acggcataga
     1381 tggaattgca cctaatgcac aaattttctc ttacaaaatg tattctgacg caggatctgg
     1441 gtttgcgggt gatgaaacaa tgtttcatgc tattgaagat tctatcaaac acaacgttga
     1501 tgttgtttcg gtatcatctg gttttacagg aacaggtctt gtaggtgaga aatattggca
     1561 agctattcgg gcattaagaa aagcaggcat tccaatggtt gtcgctacgg gtaactatgc
     1621 gacttctgct tcaagttctt catgggattt agtagcaaat aatcatctga aaatgaccga
     1681 cactggaaat gtaacacgaa ctgcagcaca tgaggatgcg atagcggtcg cttctgctaa
     1741 aaatcaaaca gttgagtttg ataaagttaa cataggtgga gaaagtttta aatacagaaa
     1801 tataggggcc tttttcgata agaataaaat cacaacaaat gaagatggaa caaaagctcc
     1861 tagtaaatta aaatttgtat atataggcaa ggggcaagac caagatttga taggtttgga
     1921 tcttaggggc aaaattgcag taatggatag aatttataca aaggatttaa aaaatgcttt
     1981 taaaaaagct atggataagg gtgcacgcgc cattatggtt gtaaatactg taaattacta
     2041 caatagagat aattggacag agcttccagc tatgggatat gaagcggatg aaggtactaa
     2101 aagtcaagtg ttttcaattt caggagatga tggtgtaaag ctatggaaca tgattaatcc
     2161 tgataaaaaa actgaagtca aaagaaataa taaagaagat tttaaagata aattggagca
     2221 atactatcca attgatatgg aaagttttaa ttccaacaaa ccgaatgtag gtgacgaaaa
     2281 agagattgac tttaagtttg cacctgacac agacaaagaa ctctataaag aagatatcat
     2341 cgttccagca ggatccacat cttgggggcc aagaatagat ttacttttaa aacccgatgt
     2401 ttcagcacct ggtaaaaata ttaaatctac gcttaatgtt attaatggca aatcaactta
     2461 tggctatatg tcaggaacta gtatggcgac tccaatcgtg gcagcttcta ctgttttgat
     2521 tagaccgaaa ttaaaggaaa tgcttgaaag acctgtattg aaaaatctta agggagatga
     2581 caaaatagat cttacaagtc ttacaaaaat tgccctacaa aatactgctc gacctatgat
     2641 ggatgcaact tcttggaaag aaaaaagtca atactttgca tcacctagac aacagggagc
     2701 aggcctaatt aatgtggcca atgctttgag aaatgaagtt gtagcaactt tcaaaaacac
     2761 tgattctaaa ggtttggtaa actcatatgg ttccatttct cttaaagaaa taaaaggtga
     2821 taaaaaatac tttacaatca agcttcacaa tacatcaaac agacctttga cttttaaagt
     2881 ttcagcatca gcgataacta cagattctct aactgacaga ttaaaacttg atgaaacata
     2941 taaagatgaa aaatctccag atggtaagca aattgttcca gaaattcacc cagaaaaagt
     3001 caaaggagca aatatcacat ttgagcatga tactttcact ataggcgcaa attctagctt
     3061 tgatttgaat gcggttataa atgttggaga ggccaaaaac aaaaataaat ttgtagaatc
     3121 atttattcat tttgagtcag tggaagaaat ggaagctcta aactccaacg ggaagaaaat
     3181 aaacttccaa ccttctttgt cgatgcctct aatgggattt gctgggaatt ggaaccacga
     3241 accaatcctt gataaatggg cttgggaaga agggtcaaga tcaaaaacac tgggaggtta
     3301 tgatgatgat ggtaaaccga aaattccagg aaccttaaat aagggaattg gtggagaaca
     3361 tggtatagat aaatttaatc cagcaggagt tatacaaaat agaaaagata aaaatacaac
     3421 atccctggat caaaatccag aattatttgc tttcaataac caagggatca acgctccatc
     3481 atcaagtggt tctaagattg ctaacattta tcctttagat tcaaatggaa atcctcaaga
     3541 tgctcaactt gaaagaggat taacaccttc tccacttgta ttaagaagtg cagaagaagg
     3601 attgatttca atagtaaata caaataaaga gggagaaaat caaagagact taaaagtcat
     3661 ttcgagagaa cactttatta gaggaatttt aaattctaaa agcaatgatg caaagggaat
     3721 caaatcatct aaactaaaag tttggggtga cttgaagtgg gatggactca tctataatcc
     3781 tagaggtaga gaagaaaatg caccagaaag taaggataat caagatcctg ctactaagat
     3841 aagaggtcaa tttgaaccga ttgcggaagg tcaatatttc tataaattta aatatagatt
     3901 aactaaagat tacccatggc aggtttccta tattcctgta aaaattgata acaccgcccc
     3961 taagattgtt tcggttgatt tttcaaatcc tgaaaaaatt aagttgatta caaaggatac
     4021 ttatcataag gtaaaagatc agtataagaa tgaaacgcta tttgcgagag atcaaaaaga
     4081 acatcctgaa aaatttgacg agattgcgaa cgaagtttgg tatgctggcg ccgctcttgt
     4141 taatgaagat ggagaggttg aaaaaaatct tgaagtaact tacgcaggtg agggtcaagg
     4201 aagaaataga aaacttgaca aagacggaaa taccatttat gaaattaaag gtgcaggaga
     4261 tttaagagga aaaatcattg aagtcattgc attagatggt tctagcaatt tcacaaagat
     4321 tcatagaatt aaatttgctg atcaggctga tgaaaagggg atgatttctt attacctagt
     4381 agatcctgac aaagatgctt ctaaatatga aaaacttggt gaaatttctg aagataaact
     4441 caaaaatgca aagagcccag aggaaaatac caataataat caagctaagg atgaagattc
     4501 aaaaccagat gaaaaaagtt cagttgaggg agaagctagc cttgaaataa ataaaactat
     4561 ttcaacaatt agagagtttg aaaataaaga cctaaagaaa ctcattaaaa agaaatttag
     4621 agaagttgat gattttacaa gtgaaactgg taagagaata gaggaatacg attataaata
     4681 cgatgataag ggaaatatta ttgcttatga cgatggtagt gccttacaat atgaaactga
     4741 aaaatttgac gaaataaaat caaaaattta tggtgttcta agcccatcta aagatggaca
     4801 ctttgaaatt cttggaaaga taagcaatgt ttctaaaaat gccaaggtat attatggaaa
     4861 tagctataaa tcgatagaaa tcaaagcgac taagtatgat tcacattcaa aaacgatgat
     4921 atttgattta tacgctaata ttaatgatat tgtagatgga ttagcttttg ctggagatat
     4981 gagattattt gttaaagatg acaatcaaat aaaagctgaa actaagatta gaatgcctga
     5041 aaaaaataag gaaactaaag cagaatatcc ctatgtatca agttatggga atgtaatcga
     5101 attaggagaa ggagatcttt caaagaacaa accagacaat ttaactaaaa tggaatctgg
     5161 taaaatctat tctgattcag aaaaacaaca atatctgtta aaggataaca tcattctaag
     5221 aaaaggctat gcactaaaag tgactaccta taatcctgga aaaacggata tgttagaagg
     5281 aaatggagtc tatagcaagg aagatatagc aaaaatacaa aaggccaatc ctaatctaag
     5341 agtcctttca gaaacaacaa tttatgctga tagtagaaat gttgaagatg gaagaagtac
     5401 ccaagctgta ttaatgtcgg ctttggacgg ctttaacatt ataaggtatc aagtgtttac
     5461 atttaaaatg aacgataaag gggaagctat cgataaagac ggaaatcttg tgacagattc
     5521 ttctaaactt gtattatttg gtaaggatga taaagaatac actggagagg ataagtccaa
     5581 tgtagaagct ataaaagaag atggctccat gttatttatt gataccaaac cagtaaacct
     5641 ttcaatggat aagaactact ttaatccatc taaatctaat aaaatttatg tacgaaatcc
     5701 agaattttat ttaagaggta agatttctga taagggtggt tttaactggg aattgagagt
     5761 taatgaatcg gttgtagata attatttaat ctacggagat ttacacattg ataacactag
     5821 agattttaat attaagctga atgttaaaga cggtgacatc atggactggg gaatgaaaga
     5881 ctataaagca aacggatttc cagataaggt aacagatatg gatggaaatg tttatcttca
     5941 aactggctat agcgatttga atgctaaagc agttggagtc cactatcagt ttttatatga
     6001 taatgttaaa cctgaagtaa acattgatcc taagggaaat actagtatcg aatatgctga
     6061 tggaaaatct gtagtcttta acatcaatga taaaagaaat aatggattcg atggtgagat
     6121 tcaagaacaa catatttatg taaatggaaa agaatataca tcatttgatg atattaaaca
     6181 aataacagac aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac
     6241 cgtaaaagaa ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag
     6301 ggtaactgtg accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga
     6361 ttttatttta cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga
     6421 aatttctggt ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac
     6481 ctttataaaa cctgtattca agaaaataga ggagaaaaag gaggaagaga ataaacctac
     6541 ttttgatgta tcgaaaaaga aagataaccc acaagtaaat catagtcaat taaatgaaag
     6601 tcacagaaaa gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga
     6661 tgttacagct acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc
     6721 taataagttg ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat
     6781 aatgtttata gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aagacaaaag
     6841 ctatagaaaa aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt
     6901 gaaaacagcc atttataact caattattta gtttacttca ctattgatac tatttggagt
     6961 tattaatgga cttagtttag ataactaatg aattgattga aagggttagt attgacaata
     7021 ttggtcatat tgattagaaa atagagtcta tcaaaattta aaggctaata gaggtgatga
     7081 gacaatttca aggaattaag tagcgtttgg ttgcttaatt cctttttatt tggaaggaag
     7141 tttttcgtaa ccgtccaata acgaagtcta ttggacggtt acgatatgat cactgacttc
     7201 gtcagtctta tctacaacct caaaactgtg ttttgagcaa cctgcggcta gcttcct
//
LOCUS       DAVKJU010000082         6796 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVKJU010000082 DAVKJU010000000
VERSION     DAVKJU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.082"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..716)
                     /locus_tag="VW191_001843"
     CDS             complement(<1..716)
                     /locus_tag="VW191_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU9624565.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIR"
     gene            complement(713..2038)
                     /locus_tag="VW191_001844"
     CDS             complement(713..2038)
                     /locus_tag="VW191_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU9624566.1"
                     /translation="MDLLGFGTVIVHFLIISHSYRLICKGRINRKELYVFGAYTLLTE
                     IVLEFSFYLLYLDKIGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDKDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVMVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            complement(2059..2184)
                     /gene="comC"
                     /locus_tag="VW191_001845"
     CDS             complement(2059..2184)
                     /gene="comC"
                     /locus_tag="VW191_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEU9624567.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRISRIILDFLFLRKK"
     gene            complement(2349..2422)
                     /locus_tag="VW191_001846"
     tRNA            complement(2349..2422)
                     /locus_tag="VW191_001846"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2386..2388),aa:Arg,seq:tct)
     gene            complement(2466..2945)
                     /gene="rlmH"
                     /locus_tag="VW191_001847"
     CDS             complement(2466..2945)
                     /gene="rlmH"
                     /locus_tag="VW191_001847"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU9624568.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            3128..4309
                     /locus_tag="VW191_001848"
     CDS             3128..4309
                     /locus_tag="VW191_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU9624569.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            4367..5125
                     /locus_tag="VW191_001849"
     CDS             4367..5125
                     /locus_tag="VW191_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU9624570.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            5338..6699
                     /gene="dnaA"
                     /locus_tag="VW191_001850"
     CDS             5338..6699
                     /gene="dnaA"
                     /locus_tag="VW191_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU9624571.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         2359 a         1216 c         1074 g         2147 t
ORIGIN      
        1 tttctaattt ttaatcttga aataggacaa cgatggtctt catagaaaac aatttctaag
       61 ttttttcgat caatttctct gatattgcct atatttacca aaaatgactt gtgaggagaa
      121 taaaatcgct gagtatgttt gtccttttcc tgaatatctg tcatggtacc ataaaactct
      181 tttgcaaaat tcttaccaat aatgcgcaat ttatgagata cccctgttgt ttcaatatac
      241 aaaatatcat ggtaaggaat ttttaaatca tttcccttgt aattgtagtc gaaataatct
      301 acaacatctt cattttcaag taacatactc ttcgtgtaga agatattttg ctcaattctc
      361 ttcttaaaca tctcatcatt gatatcctta tcaacaaaat ctagggctga tacctggtat
      421 ttataggtta gagtcgcaaa ctctgatcga ctagtgataa agacgataat agcgtaagga
      481 ttgtaatgac gaatgagctg agccacttca aatccctttt tctcaattcc atgaatatcg
      541 atatctagga aataaagctg atttacttca tcattttcaa tgtattcttc aaattcacgg
      601 acttttcccg ttgtcttgta tgatattgga atattcgatt ctttcgaaat ttcatccaat
      661 attctctcta gtctcacttg atgttcaata acatcttcta aaattaaaac tttcattcaa
      721 attccctctt aaatctaatg atttgtctaa atgtactgcc ttccatctct gtttctaaaa
      781 taatattgtt gtacttatct agtagttctt tcacattatt taatccgact ccgcgatttc
      841 ttcccttagt ggagaatcct aaggcaaata gatctcctga aggagtcatc gtcattttac
      901 atgaattctg aatcaccata actgtttcag tttccatctt aataactgct acttccatct
      961 gctttttata gctatcagcc gatccttcga cagcattatt caataaaacg ctcatgatac
     1021 gaaccaaatc caatagttca attggaagct tggtaatcgt atcttttact tccagtgtaa
     1081 actctacacc attatttcga gcatagacaa ttgactgagc aaccaaactt cgtaaagctg
     1141 agtcttctat gttgttcaaa tcaaagtaag tgtacttatc tgaacgcaat ttatgatttg
     1201 ctttgactaa aacttcattg taaattctgt caatttcctg taaattacca ctgtcaattg
     1261 ccatctgcat gctgacaagc attccagcat aatcatgtcg aaaaccacgg atttcattat
     1321 acagaccaac aatttcatct gtgtaattct gtaaatgttt ctgttcaaat ttcttctgct
     1381 tcaaagcaat ctctttctcc atttgaactt tatgagaatt cattgcaaag aaggtcaaaa
     1441 ggagagagat aaagacaata gatgacaaaa tacttccaaa actattcaaa tgtttaatcg
     1501 tacttaccat atctgaaacg aaagatacaa tatgtagcaa tagtaaagca aaaaatactt
     1561 ttttcaagaa aggataaagg tagtccttgt caaaataggc tagttccaaa tggaaataat
     1621 aaatgatttt taatgtaaca aaataggtta acaccgtcac aacgaaaaag aatgggaaat
     1681 gatattgtaa aacaaaatta tctcctgtta tagaggagaa agttacggac agaaagttat
     1741 gagtgctctc atataaaaga gatagtagta aacttaggaa tagtcctcta tccctctcat
     1801 actgtttcat ccatcgaaaa taggaatata agcccaaagg aaataaaaat ctttcaatcc
     1861 ctattttatc taaatataga agataaaagg aaaattcaag tactatttca gttagtaatg
     1921 tataagcacc aaaaacgtat aattcttttc tatttattcg acctttacaa attaaacggt
     1981 aactgtgact aataattaaa aaatgaacaa taactgtccc aaatccaagt aaatccatta
     2041 ctctttctcc ttatttcatt acttttttcg taggaaaaga aaatcaagga tgattcttga
     2101 aatcctcatc tccccacctt taatcttttg taagtctttt tccttcaaag ctacaaactg
     2161 ttccaattta actgtgtttt tcataataaa atctcctaaa atgttttttc ttgtaagcta
     2221 acttacaaaa accattatac aaaatggaat ttcgttttag ataaaattct ctcaactgtc
     2281 atttttttct cccaaagtgt acttttttaa gaaaaaagcc aggaaaattc ccagctttgc
     2341 tattatattg atcccagcag gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc
     2401 tatccagctg agctatgaga cctaacacaa ttattctacc aaaaattcaa ttaaaagtca
     2461 attttctatt tatggtaggg gaatccctgc tgaatcgtaa aagcgcgata gatttgttca
     2521 acaagaacta gtctcattaa ctgatgaggc aaggttaggc gaccaaaact gacagaaaga
     2581 ttggctctat tttttacaga tgatgataat cctaaacttc ccccaataat aaaagtaaga
     2641 gtagaaaatc cttttataga agtttcttct aactgcttac taaattcttc tgagaagaaa
     2701 gttttccctt caatggctaa cacaataacg aaatcacggt cagcaatttt tgataaaatt
     2761 ctctgacctt ctatttctaa aatcttttga ttttctgatt cactggcctt atctggtgtt
     2821 ttttcatctg ataactcaat catttcaaac ttagcaaatc tagaaattcg ttttgaatac
     2881 tctgcgatac catcttttaa atacttttct ttcagtttcc caactgttac aactttaatt
     2941 ttcatgactc tattctaaca tattctctat tttttcacat cttattcaca aaataaaaaa
     3001 tagatttcaa ttaagaaaat cacagtttca aaagagttat ccacagtttg tgtaaaactt
     3061 ttgtgtttaa gttataatta agctagtcag tttatacttt cagtaattca aacatatgga
     3121 ggcaaatatg aaacatctaa aaacatttta caaaaaatgg tttcaattat tagtcgttat
     3181 cgtcattagc ttttttagtg gagccttggg tagtttttca ataactcaac taactcaaaa
     3241 aagtagtgta aacaactcta acaacaatag tactattaca caaactgcct ataagaacga
     3301 aaattcaaca acacaggctg ttaacaaagt aaaagatgct gttgtttctg ttattactta
     3361 ttcggcaaac agacaaaata gcgtatttgg caatgatgat actgacacag attctcagcg
     3421 aatctctagt gaaggatctg gagttattta taaaaagaat gataaagaag cttacatcgt
     3481 caccaacaat cacgttataa atggcgccag caaagtagat attcgattgt cagatgggac
     3541 taaagtacct ggagaaattg tcggagctga cactttctct gatattgctg tcgtaaaaat
     3601 ctcttcagaa aaagtgacaa cagtagctga gtttggtgat tctagtaagt taactgtagg
     3661 agaaactgct attgccatcg gtagcccgtt aggttctgaa tatgcaaata ctgtaactca
     3721 aggtatcgta tccagtctca atagaaatgt atccttaaaa tcggaagatg gacaagctat
     3781 ttctacaaaa gccatccaaa ctgatactgc tattaaccca ggtaactctg gcggcccact
     3841 gatcaatatt caagggcagg ttatcggaat tacctcaagt aaaattgcta caaatggagg
     3901 aacatctgta gaaggtcttg gtttcgcaat tcctgcaaat gatgctatca atattattga
     3961 acagttagaa aaaaacggaa aagtgacgcg tccagctttg ggaatccaga tggttaattt
     4021 atctaatgtg agtacaagcg acatcagaag actcaatatt ccaagtaatg ttacatctgg
     4081 tgtagttgtt cgttcggtac aaagtaatat gcctgccaat ggtcaccttg aaaaatacga
     4141 tgtaattaca aaagtagatg acaaagagat tgcttcatca acagacttac aaagtgctct
     4201 ttacaaccat tctatcggag acaccattaa gataacctac tatcgtaacg ggaaagaaga
     4261 aactacctct atcaaactta acaagagttc aggtgattta gaatcttaat tgacatctat
     4321 gtaaagaaag ctttacataa gagaaaagat gtgttagtgt agaatcatgg aaaaatttga
     4381 aatgatttct atcacagata tacaaaaaaa tccctatcaa ccccgaaaag aatttgatag
     4441 agaaaaacta gatgaactag cacagtctat caaagaaaat ggggtcattc aaccgattat
     4501 tgttcgtcaa tctcctgtta ttggttatga aatccttgca ggagagagac gctatcgggc
     4561 ttcactttta gctggtctac ggtctatccc agctgttgtt aaacagattt cagaccaaga
     4621 gatgatggtc cagtccatta ttgaaaattt acagagagaa aatttaaacc caatagaaga
     4681 agcacgcgcc tatgaatctc tcgtagagaa aggattcacc catgctgaaa ttgcagataa
     4741 gatgggcaag tctcgtccat atatcagcaa ctccattcgt ttactttcct tgccagaaca
     4801 gattctctca gaagtagaaa atggcaaact atcacaagcc catgcgcgtt ccctagttgg
     4861 gttaaataag gaacaacaag actatttctt tcaacggatt atagaagaag atatttctgt
     4921 aaggaagtta gaagctcttc tgacagagaa aaaacaaaag aaacagcaaa aaactaatta
     4981 tttcatacaa aatgaagaaa aacagttaag aaaactactc ggattagatg tagaaattaa
     5041 actatctaaa aaagacagtg gaaaaatcat tatttctttt tcaaatcaag aagaatatag
     5101 tagaattatc aacagcctga aataaggctg ttcttttatt tttttatctc acaaggttat
     5161 ccactatgtt tttcgataaa aagcttaata aatcaataat ttcttctttt atccccaacc
     5221 tgtggataaa gtttagtaac attgtggatt atttttcaca gcttgtggaa aattcttgct
     5281 atctatggta aaatatctct agtattaaac ttttaaatag taaaggagga gaaaggattg
     5341 aaagaaaaac aattttggaa tcgtatatta gaatttgcac aagaaagact gactcgatcc
     5401 atgtatgatt tctatgctat tcaagctgaa ctcatcaagg tagaggaaaa tgttgccact
     5461 atatttctac ctcgctctga aatggaaatg gtctgggaaa aacaactaaa agatattatt
     5521 gtagtagctg gttttgaaat ttatgacgct gaaataactc cccactatat tttcaccaaa
     5581 cctcaagata cgactagctc acaagttgaa gaagctacaa atttaactct ttatgactat
     5641 agtccaaagt tagtatctat tccttattca gatacgggat taaaagaaaa gtataccttt
     5701 gataacttta ttcaagggga tggaaatgtt tgggctgtat cagccgcttt agctgtctct
     5761 gaagatttgg ctctgaccta taaccctctt tttatctatg gaggaccagg ccttggtaag
     5821 actcacttat taaacgctat tggaaatgaa attctaaaaa atattcctaa tgcgcgtgtt
     5881 aaatatatcc ctgccgaaag ctttattaat gactttcttg atcacctaag acttggggaa
     5941 atggaaaagt ttaaaaagac ctatcgtagt cttgatcttt tgttaatcga tgatatccag
     6001 tcactcagcg gaaaaaaagt cgcaactcag gaagaatttt tcaatacctt taacgccctt
     6061 catgacaagc aaaaacagat tgtcctaacg agtgatcgta gtccaaaaca tctagaaggg
     6121 ctcgaggaga ggcttgtcac gcgttttagt tggggattga cacaaactat cacaccccct
     6181 gactttgaaa cacgtattgc cattttacaa agtaaaacgg aacatttagg ctacaatttc
     6241 caaagtgata ctctagaata cctagctggg caatttgatt caaatgttcg agatcttgag
     6301 ggagccatca acgacatcac tttaattgcc agagtaaaaa aaatcaagga tatcactatt
     6361 gatattgctg cagaagccat tagagcccgc aaacaagatg ttagccaaat gctcgtcatc
     6421 ccaattgata aaatccaaac tgaagttggt aacttttatg gtgttagtat caaagaaatg
     6481 aagggaagta gacgccttca aaatattgtt ttggcccgtc aagtagccat gtatttatct
     6541 agagaactaa cagataatag tcttccaaaa attgggaagg aatttggggg aaaagatcat
     6601 accacagtca ttcatgccca tgccaaaata aaatctttga ttgatcaaga cgataattta
     6661 cgtttagaaa ttgaatcaat caaaaagaaa atcaaataat ttgtggataa cttttagttt
     6721 tttatctttt ttatccacat tttttaaaca agctaaaaaa cttgatatga cttgtttaaa
     6781 ggctgttttc cacaga
//
LOCUS       DAVKJU010000083         6760 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVKJU010000083 DAVKJU010000000
VERSION     DAVKJU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6760
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.083"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..293)
                     /locus_tag="VW191_001851"
     CDS             complement(<1..293)
                     /locus_tag="VW191_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9624572.1"
                     /translation="MNRLKELRQEKKLSQKEIAETLGFSLRSFQRMENGESQIKPEKA
                     QLLADYFGVSVANLLGYENNFIESVKNLSQKDGSDEVFFKAFRAYYELKTADG"
     gene            513..713
                     /locus_tag="VW191_001852"
     CDS             513..713
                     /locus_tag="VW191_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU9624573.1"
                     /translation="MSKLRGYRVMLGLTQQQMADKLKISLQSYNNKELGKTPFNDKER
                     LAIKSMVAEIKPDITIDEIFYS"
     gene            898..1326
                     /locus_tag="VW191_001853"
     CDS             898..1326
                     /locus_tag="VW191_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624574.1"
                     /translation="MGKKHQVVKFKDIAEKLPELEGKNLEEIAGVLGYRNLESCRVNL
                     YNLRQNKRLGFEVEKGVYTKFELLDDTVKEELEEKELSERGHFLQSLDYYKVAKNAFE
                     IAEDKTVKAETRQKAERDILDAMKHIPKKHRAILYDMMEG"
     gene            1673..2125
                     /locus_tag="VW191_001854"
     CDS             1673..2125
                     /locus_tag="VW191_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624575.1"
                     /translation="MTETTYDIIAKSLDRISMELHQADENNDFLRIGLLSGQLKAIKE
                     NLHRLLWIELPELNESHKIEAVSKSTTGIFFHPGIFEMDAMRQAFFKRQAKHFFDNAT
                     EQQEYIEYAEKEYLEATKTLKDILFNTNNGTRQVNKDCLVEKFEEAMQ"
     gene            2122..2301
                     /locus_tag="VW191_001855"
     CDS             2122..2301
                     /locus_tag="VW191_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624576.1"
                     /translation="MTQSEFYNRMTKIKDRHPNLFQFIIDFLDDKVSTEEVYDFLKME
                     RSYQVNHIKNYKARA"
     gene            2303..2518
                     /locus_tag="VW191_001856"
     CDS             2303..2518
                     /locus_tag="VW191_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624577.1"
                     /translation="MNELDLSNTQAVFVSVVLIGILLYLNHRDRKKSAQIERENQQTI
                     ETPSDDLNPDYGRYIQLAGVRVSGGME"
     gene            2515..2808
                     /locus_tag="VW191_001857"
     CDS             2515..2808
                     /locus_tag="VW191_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624578.1"
                     /translation="MSYTVKIYLDFEKIPDETYFTKEIFLAMCTDNATKLAVTMGLLA
                     KQALDLETRAVILKTLNTIIENQKAILQGVASGQITFLNKSKGEEELDESIRE"
     gene            2789..3130
                     /locus_tag="VW191_001858"
     CDS             2789..3130
                     /locus_tag="VW191_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9624579.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGLEISLTTKLTERTVQDIIKR
                     LIIKHNIPIVGVRNGFYRGYFIPRNKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            3123..3395
                     /locus_tag="VW191_001859"
     CDS             3123..3395
                     /locus_tag="VW191_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624580.1"
                     /translation="MFSLSRESEKDLTHGILEVVERYLEAHEKVPPRLTKLINRIELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVDE"
     gene            3392..3907
                     /locus_tag="VW191_001860"
     CDS             3392..3907
                     /locus_tag="VW191_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001263812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624581.1"
                     /translation="MRVIELILSADKLPLFGFLKSTPTQVWKNGEHYKFIYFEPIGEG
                     LTAFHYKGLYVAVKDENEEVEGWELTRDLEIGLASPDLLMILKNLEVNKLTEQRQGLG
                     VELKGWVFNLICNGIYTRYETSLFVRLLFVNGYSFSQLVDLFSAIVKRKDLASYFLEV
                     ATKFYKEVAFE"
     gene            3900..5351
                     /locus_tag="VW191_001861"
     CDS             3900..5351
                     /locus_tag="VW191_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEU9624582.1"
                     /translation="MNSNDIVNKIIEENQQEAPPEVVDLTQARKTNEEHNSLNLAKRP
                     RGDGFDRGLDNLRKILSGDSKLKGAIQYNTFTYEIEVTRPMKLNGRTLNGAIDDLIIR
                     EIRAYIATKYKLDYKKPDIADFLEIVAGENSYNPLKDYLESCESEYKELVNQREPFDI
                     LRHYLNIKDDEYNRIIMDLFFRGAVAKVFDPTVKFDFVLDLTGRQGVGKTQFFEGLFT
                     HKYFTTVETFTDKDDKARMVRNWCVFDDEMVASKKASFSELKKFITETKLEFRPPYAS
                     SDRRLPKSFIIVRATNDHDYLNDLTGERRFLVVEVHKDTAYKGRKWTEKDRRAFWGAM
                     VVAWRANQVLNLTDEQEKLVNEVRSRYKFVDEILEDLERYLDTPYPKRMYQFPMTDRM
                     RYYYIYDMMNEGYYRNNRGGTVELDTDTYGELVDRDKMTINLFFQEVYLTDKAPPKDK
                     AKVKKYMQNRDGWEHRRSTRFGKSIKPAYVKKM"
     gene            5890..6054
                     /locus_tag="VW191_001862"
     CDS             5890..6054
                     /locus_tag="VW191_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEU9624583.1"
                     /translation="MNPNKDYTPFLQDNFIIFNKNGIIEMKKIPDFGSVVFTSQDGNI
                     VQIETTIKER"
     gene            6208..>6760
                     /locus_tag="VW191_001863"
     CDS             6208..>6760
                     /locus_tag="VW191_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624584.1"
                     /translation="METLQTIETKIDKLIEQNKKAIEMTEAELVKASQAISDAQAKLV
                     QAQEEINSEKYVEAKSDLWTAERTKEFHEGRLKELVTTPIISYDEYHATVKEIYRLAD
                     EQQNTFFTPAVAKLLEIVKLGDESAKEVGKVNEIFRKLEKDISKNNEDYKRDKNGGWF
                     RGPFSSLSYSPRNALHGYQDDLKE"
BASE COUNT         2449 a         1083 c         1478 g         1750 t
ORIGIN      
        1 ctaccatcag ctgtttttag ttcatagtat gctctaaatg ctttaaaaaa tacttcatca
       61 gagccatctt tttgacttaa attttttaca gattcaataa aattgttttc ataaccgagt
      121 aagttagcaa cgcttacccc gaagtaatca gcaagtagtt gggctttttc tggtttaatt
      181 tggctttctc cgttttccat tcgttggaat gaccttaaag aaaatcccaa agtttcagct
      241 atttcttttt gagatagctt tttttcttgt cttaattctt tcaatctatt catgttttat
      301 cacacctttc acagatgatt ataaccttat gtttaaaaaa agtcaaaaaa tgacgtaaaa
      361 aaatcaaaaa aactattgac aacgtctaaa actgacgtta taataaaaat caagcttatg
      421 ttgcgtcata aaatgacgtc aagcaccccc tccaaccttt cacactttca atctattcat
      481 ggagggggat ttttttagaa aggagcaaat ctatgagcaa actacgaggc tatcgggtta
      541 tgttaggact aactcagcaa caaatggcgg acaagctaaa aatttctttg cagtcgtaca
      601 acaacaaaga attaggtaaa acgccattca atgacaaaga acgtctagcg attaagtcaa
      661 tggttgcaga aatcaaacca gacataacaa tagacgaaat attttatagt tagaaaggag
      721 cgaactaatc gcaatactac tctatattta cagatttctc atgtggtgct ttaccactgg
      781 ggattgacaa caaaaaagtc acttgctgaa tgtttggcga cagaagcaag cgactggatc
      841 aagagtatag atattttttc tataccttga ttatagcata gaaagacaag gtaaacaatg
      901 ggaaagaaac atcaagtagt aaaatttaaa gatatagctg aaaaattgcc tgaattagaa
      961 ggtaaaaatt tagaagaaat cgctggagta ttaggctacc gcaacttgga gagttgcagg
     1021 gtcaaccttt acaatctcag acaaaacaag cgtctagggt ttgaagtaga aaaaggggtt
     1081 tacactaagt tcgaactctt ggacgatact gtaaaagaag aactggaaga aaaagaattg
     1141 tctgaacgtg gtcatttttt acagtcgcta gactactaca aagtagcaaa aaacgccttt
     1201 gaaatagcag aagataaaac agtaaaagca gaaacccgac aaaaagcaga gcgtgacata
     1261 ttggacgcga tgaagcacat tcctaaaaaa catcgcgcta tactttatga catgatggag
     1321 ggctaggatg gaagcaaaaa cccacttcgc tagattcatg cgtagaggca tggagttagc
     1381 aaggcaatta catagtaaag aaattcaacg tgatgaattt gatagagcct ggaaaagatt
     1441 aggcgattaa atcgaaaacg aaacgaagaa aaactaagaa aaccgaagag caggcaagca
     1501 attagaaaag gttttgaaat cgagtgctga cacgacgatt ctaagcactt gtttagaaaa
     1561 aatgtgggtg attacccacg aaacatcact acaagcgtcc gccaacttgg ggcaaatgcc
     1621 cagcgtttgg agtggtgaaa taatccaata taggaaaaag ggaaaacaga ctatgacaga
     1681 aacaacatac gatattatcg ctaaaagctt ggatagaatt agtatggaat tacaccaagc
     1741 agacgaaaac aatgattttt tgagaatagg actcttatca ggacaattaa aagctatcaa
     1801 agaaaattta caccgcttac tttggattga gcttcctgaa ttgaatgaaa gtcataaaat
     1861 cgaggcagtc tctaaaagta ctactggaat ctttttccat ccaggtattt ttgagatgga
     1921 tgcaatgaga caagcattct ttaaacgtca agccaagcat ttttttgaca atgcaacaga
     1981 gcaacaggag tatatagaat atgctgaaaa ggagtattta gaggctacta aaaccttgaa
     2041 ggatatcctt tttaacacta ataatgggac tagacaagta aataaagatt gtcttgtaga
     2101 aaaatttgaa gaggcaatgc aatgacacaa tcagaatttt acaaccgaat gactaaaatc
     2161 aaggatagac atccgaacct ctttcagttc atcattgact ttttagatga taaagtaagc
     2221 accgaagagg tgtatgattt tctgaagatg gagcgaagct atcaagtaaa tcatatcaag
     2281 aattacaaag cgagagcata acatgaacga actagattta agcaacacac aggcggtttt
     2341 tgttagcgtg gtactgattg gcatactgct ttatctaaac caccgagacc gcaaaaaaag
     2401 cgcccaaatt gagcgagaaa accaacagac gatagaaaca cctagcgatg atttaaaccc
     2461 tgattatggg cgatatatcc agcttgcagg ggtaagagtt tccggaggga tggaatgagt
     2521 tacacagtga agatatacct tgattttgaa aaaataccag atgaaaccta ttttactaaa
     2581 gagatttttc tagctatgtg tacggacaat gcaacaaagt tagctgtaac tatgggtttg
     2641 ctggcaaaac aagcgttgga tttagagact agggctgtta ttttaaaaac ccttaataca
     2701 atcatagaaa atcaaaaagc tattttgcaa ggggtagcga gcggacaaat aacctttttg
     2761 aataaaagta aaggagagga ggaactggat gagtctatca gagaatgaca agcgagtttt
     2821 aagactaatc aaggtagggg ctgagaactc cataacaggg ttagaaatca gcctgacaac
     2881 caagctaaca gaaagaaccg tgcaagatat tattaagcgt ttaataatca agcataacat
     2941 cccaattgtg ggggttagaa atggatttta tagaggttat ttcataccta gaaacaaagg
     3001 cgagttacta gatggtgcta aggcttttta caaccaagta caggaggaga gcaagcgcct
     3061 agcggtgttg atgaatagcg atttagaaag ctacaaagaa accttgaagg aggtgggcgg
     3121 atatgtttag cctaagccga gaaagcgaga aagacctaac gcatggcatt ctggaggtag
     3181 tggaaagata cctggaagcg catgagaaag taccgccaag attgaccaag ttaataaata
     3241 ggattgaact caaagaggaa ttgaaaatta gtgacaacac gctgaataag tgggaaagtc
     3301 agggtttaag acgctatcaa ccgccggacg atggttcaag aatgatttac tatttagtaa
     3361 ctgacatctg gaagtttctg ggggtggatg aatgagggtg atagagttaa ttttatccgc
     3421 tgacaaattg cctttgtttg gctttctcaa gtctacgcca acgcaagttt ggaagaatgg
     3481 ggaacactac aaatttatct attttgagcc aataggcgag ggcttgacgg ctttccacta
     3541 caaaggtttg tatgtggcag ttaaagacga aaacgaggaa gtagagggct gggaactaac
     3601 cagagattta gaaataggtt tggctagtcc tgacttgctg atgatcctga aaaatttaga
     3661 ggtaaataaa ttgacagagc aacggcaggg gcttggagtg gagttaaaag gctgggtttt
     3721 taacctgatt tgtaacggca tttataccag atatgagact tcgctttttg tccgcttgct
     3781 atttgtcaat ggctacagtt ttagtcagct ggtggacttg ttttcagcaa tcgtcaaacg
     3841 caaagaccta gcaagctact ttctagaagt agcgacaaaa ttctataagg aggtggcttt
     3901 tgaatagcaa tgacattgta aataaaatca ttgaagagaa tcagcaagaa gcaccgcctg
     3961 aagtggtgga cttgacccaa gcaaggaaaa ccaacgagga acacaatagc ctgaacttgg
     4021 caaagagacc aagaggggac ggctttgata gaggtttaga caatctgaga aagattttga
     4081 gcggagatag caagctaaaa ggggcgatac agtacaacac ttttacatat gaaattgaag
     4141 tgactagacc aatgaagcta aacggtagaa ccctgaacgg tgcaatcgat gacctgatta
     4201 tcagagagat tagggcttat attgctacca agtataagct agactataaa aagcctgata
     4261 tagctgattt tttggagata gtggctggag aaaatagcta caacccctta aaagactatc
     4321 tggaatcttg cgaaagcgag tataaagagt tagtgaatca acgtgagccc tttgatattt
     4381 taaggcatta tcttaatatc aaggatgacg aatataaccg tattatcatg gatttgtttt
     4441 tccgtggagc ggttgccaag gtgtttgacc ctaccgttaa gtttgacttt gtgctggact
     4501 tgactggaag gcagggagta ggaaagacac aattttttga ggggcttttt actcacaagt
     4561 attttacaac cgttgagaca ttcacagaca aagacgacaa ggctagaatg gtgagaaact
     4621 ggtgtgtctt tgatgatgag atggtggcca gtaaaaaggc tagtttttca gaattgaaga
     4681 aattcatcac agaaaccaag ctagagttta gaccccctta cgcttccagt gacaggcgat
     4741 tgcctaagag ttttatcatt gtcagagcaa ctaatgacca tgattatttg aacgacctga
     4801 caggggaaag gcgctttcta gttgtagaag tccacaagga caccgcctat aagggcagga
     4861 aatggacaga gaaagaccgc agagcctttt ggggcgctat ggtagtagct tggagggcta
     4921 accaagtctt gaacctgacg gacgagcagg aaaagctagt aaatgaggtt agaagtcgtt
     4981 acaagtttgt agatgaaatt cttgaggatt tggagcgcta tttagatact ccttacccaa
     5041 aaagaatgta tcagttcccg atgaccgaca gaatgcgcta ctattatatc tatgatatga
     5101 tgaacgaggg atactataga aataacaggg gtggaacagt tgaacttgac acagacacct
     5161 atggcgaact ggtagatagg gacaagatga ccattaacct attttttcaa gaggtgtatt
     5221 tgactgacaa agcaccgcca aaggataagg ctaaggttaa aaagtatatg caaaaccgag
     5281 acggctggga acataggcga tcaacaagat ttggtaaaag tattaaacca gcttacgtta
     5341 aaaaaatgta gtcagtgtag tttattttta aaagtgacta cattttaaac aggaagaaaa
     5401 cccttgatac ataaggggtt agaaaaaagt gttgttttta ctattttact gactacaccc
     5461 ctaaaccctt ggtattattg acttttatat aaaaatgtag ttattttcta tttattaaag
     5521 agtatatgaa gataagagat aaaaggcgtt tattgtttat ttttttggga gcaaggtgac
     5581 tacatgacta cgcatggcga aaagccttgt gactgtaagg cggagagcgt agtcactaga
     5641 tatcctaaaa tgactacact ttttggaatt gtgaggagga gacctaaaaa gaaatggaaa
     5701 tagtaactta aatgagtaac ttttaagact gaaatcatat ttgtatttta cataatgaga
     5761 aaatgtaaat aggaaatcac aaaaatagcc ctcctccctt gatagacaag gcgagaaggg
     5821 aggtcgctta aatttaacag aatagaaggt atgttaaata tatggcacca aaaaagagag
     5881 gaaaaacaga tgaatccaaa caaagattac actccatttc tacaggataa ctttattatc
     5941 tttaacaaaa atggtataat agagatgaag aaaataccag actttggtag cgtggtattt
     6001 actagtcaag atggcaacat tgtccagatt gaaacaacaa ttaaagaaag atagctgact
     6061 agacaactag aggcgtaaca ttaaagctaa gtagcttttt tgttgcgtct ctttttgttt
     6121 tgagaaagag gaagtaatga ggaataaaga cccagtaaag agttgaaacc aataagcaca
     6181 atcaaaagaa atgaggaata aaagaacatg gaaacattac aaactattga aactaaaatt
     6241 gacaaactga ttgaacagaa taagaaagct attgagatga ctgaggcgga actggttaaa
     6301 gcaagccaag ctatttcaga cgcccaagcc aagctagtac aggctcaaga agaaataaac
     6361 tctgaaaaat acgtcgaggc aaaaagtgac ctatggacgg cagaacgtac aaaggaattt
     6421 catgagggac gtttgaaaga attagttaca actccaataa tttcatatga tgaatatcac
     6481 gcaacggtta aagagattta tagattggct gatgaacaac aaaatacttt ctttactcca
     6541 gcagtagcca aactattgga aattgtaaag ctgggtgatg agtccgccaa agaagttggt
     6601 aaagtaaacg agatatttag aaaacttgaa aaagatatct ctaaaaataa cgaagattat
     6661 aaaagggaca aaaacggtgg atggtttcgt gggccatttt caagtttatc ctattctcca
     6721 agaaacgcct tacatgggta tcaagatgat ttaaaagaaa
//
LOCUS       DAVKJU010000084         6649 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVKJU010000084 DAVKJU010000000
VERSION     DAVKJU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.084"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..415
                     /locus_tag="VW191_001864"
                     /pseudo
     CDS             1..415
                     /locus_tag="VW191_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            570..770
                     /locus_tag="VW191_001865"
     CDS             570..770
                     /locus_tag="VW191_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624585.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            795..1211
                     /locus_tag="VW191_001866"
     CDS             795..1211
                     /locus_tag="VW191_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624586.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1442..2149
                     /gene="ccdA2"
                     /locus_tag="VW191_001867"
     CDS             1442..2149
                     /gene="ccdA2"
                     /locus_tag="VW191_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEU9624587.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            2160..2717
                     /gene="sdbB"
                     /locus_tag="VW191_001868"
     CDS             2160..2717
                     /gene="sdbB"
                     /locus_tag="VW191_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEU9624588.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            3092..4483
                     /locus_tag="VW191_001869"
     CDS             3092..4483
                     /locus_tag="VW191_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU9624589.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4626..5543
                     /gene="adcAII"
                     /locus_tag="VW191_001870"
     CDS             4626..5543
                     /gene="adcAII"
                     /locus_tag="VW191_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEU9624590.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTENNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5551..>6649
                     /gene="phtB"
                     /locus_tag="VW191_001871"
     CDS             5551..>6649
                     /gene="phtB"
                     /locus_tag="VW191_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9624591.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLIFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEKRIA
                     RIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPS"
BASE COUNT         2043 a         1220 c         1393 g         1993 t
ORIGIN      
        1 gcttctgaat ttggctgtca tccaactacc atccactatg cgctcaaagc tatgggctac
       61 actcgaaaaa aagaaccaca cctactatga acaagagcca gaaaaagtag ccttatttct
      121 taagaatttt aatagtttaa agcacctagc acctgtttag attgacgaaa caggattcga
      181 tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaagaa gaggcaaagt
      241 atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg gtgagttaat
      301 cgctccaatg acttacgaag agatgatgac gagcgacttt tttgaagctt ggtttcagaa
      361 gtttctctta ccaacattaa ccacaccatc ggttattata gtaaaacgaa ataagaatag
      421 gacaaatcga tcaggacagt aaaatcgaat tctaacaatg ttttagaagt ccatatgtac
      481 tattctagtt tcaatatacc ataactgctc ctagtcaacc tgcaagtcct gtcccagctc
      541 caagctcagc tccgactcaa gatcctagag tggtagtaag cgcttcggct agctcaacga
      601 gcccacaagc tcaagagcaa gttgacaagt ctgaacttcg tgccttgagt caagagttag
      661 accaacactt gaaagctttg gcaacagtat ctgatccgaa aatcgatgca accaaggctg
      721 tcctcctaga tgctcaaaaa gctccggaag atagtgcctt gacagagtaa ggtctgcata
      781 ctgcagtaga gtctgtgaag gctgccttgg actctctcaa agatgtgaaa gcaaatgcta
      841 gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca aggaacggaa gatagtaagg
      901 attcagataa gatgactgaa acaaactcag ttccggcagg agtgattgtg gtcagtctac
      961 ttgccctcct aggcgtgatt gccttctggc tgattcgccg taagaaagag tcagaaatcc
     1021 agcaattaag cacggaatta atcaaggttc taggacagct agatgcagaa aaagcggata
     1081 aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac ccttgatttc gtgaaagaag
     1141 aaaatggctc agcagagaca gaaactaaac tagtagagga gcttaaagca atccttgaca
     1201 aactcaagta aaacatgatt tatgaaatgt cagacttaaa agacattttc gttcacaaaa
     1261 gcagcgacga gtaatccttg tcgttgcttt ttggctattt tgggggataa gatccgcctt
     1321 gactagcaaa agaaaagatt ggtccaatag tgcccgatac gattttgcgt agcagaaagc
     1381 ttacaaataa aattgaatat agtaaaatag gattagaatt attaagaaag ttggttcttt
     1441 attggaaacg atagtatttt taatctctgt ttttctagca ggtgttttat cctttttttc
     1501 tccttgtatt tttcctcttc tgccagtcta tgctgggatt ttattggatg atcaagaaag
     1561 tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg tcaggcttga ttcgaacact
     1621 ttgctttatc gctggtatct ctctcatttt ctttattcta ggctttggtg ctggttactt
     1681 tggtcatatt ctctatgcaa attggtttcg atatggcatg ggagctatta ttatcatttt
     1741 gggtcttcat cagatggaaa tttttcattt gaagaaatta gaagttcaaa aaagttttac
     1801 ctttaaaaaa tcagattcta atcgttattg gtcagctttt ttacttggta ttacctttag
     1861 ctttggttgg acaccttgta ttggtccagt tttaagttct gttttagcac ttgcggcttc
     1921 tggaggcaat ggcgcttggc aaggagcgat ttatactctc atttacactc tgggcatggc
     1981 ccttcctttc ttggtattgg cactagcttc aggtctagtc atgccatatt ttagtaaaat
     2041 caagcgtcat atgatgctac taaagaaaat tggtggtttc ctcattgttt taatgggaat
     2101 tttgttacta ttaggacaag taaatgttct agctggaatt tttgaataaa ggaggacaaa
     2161 tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt agttgtattg atggcctgtg
     2221 gtgaggaaga aactaaaaag actcaagcag cacaacagcc aaaacaacaa acgactgtac
     2281 aacaaattgc tgttggaaaa gatgctccag acttcacatt gcaatccatg gatggcaaag
     2341 aagttaagtt atctgatttt aagggtaaaa aggtttactt gaagttttgg gcttcatggt
     2401 gtggtccatg caagaaaagt atgccagagt tgatggaact agcggcgaaa ccagatcgtg
     2461 atttcgaaat tcttactgtc attgcaccag gaattcaagg tgaaaaaact gttgagcaat
     2521 tcccacaatg gttccaggaa caaggatata aggatatccc agttctttat gataccaaag
     2581 caaccacctt ccaagcttat caaattcgaa gcattcctac agaatattta attgatagcc
     2641 aaggaaagat tggaaagatt caatttggtg ctatcagtaa tgcggatgca gaagcagcat
     2701 ttaaagaaat gaactagaat cgataaaatc tgattacaag atgtagtcag attttggtaa
     2761 cttctaaaac taacttctag tttcccacaa cctagctttt agtatgagaa aaacgtgtga
     2821 caccagtgga aatccgtctt agactaactt ccactggttt tctttttctt gatatataag
     2881 ggttcaaaag cgaaaagact cagaaaaaag tgcacgacaa atagccctaa aacagagtcg
     2941 ttttagagta acttccagtt ttgggagaga gatggaagtt actttgagaa gttacggtag
     3001 tcagattttt ttagaaaaac attttataaa tattcacata tctcctatat ttatggtaaa
     3061 atagaatcat cagtttattt tggagtcaaa gatgaatata tttagaacaa agaatgttag
     3121 tttagataaa acagagatgc ataggcattt gaagttatgg gatttgattt tgctgggtat
     3181 tggagccatg gtagggacag gtgtctttac aatcacaggt actgcagctg ctacacttgc
     3241 tggtccagca ctagtgattt caatcgttat ttctgccttg tgtgtgggat tatcagccct
     3301 cttttttgca gaatttgcct cgcgagtacc cgctacagga ggtgcctata gttacctcta
     3361 tgctatctta ggagaattcc ctgcctggtt ggctggttgg ttaaccatga tggagttcat
     3421 gacagccata tcaggcgtag cttcgggttg ggcagcttat tttaaaggct tgctctctca
     3481 atacgggata gcccttcctc aggctttaaa tggtaccttt aatcctcaag cagggacatt
     3541 tgttgattta ttgcctattc ttgtcttggt cttggtgact tcgcttgttt tactaaatgc
     3601 caaggcagcc ttacgcttta attcgattct agttattttg aaattttccg ctttagctct
     3661 ctttgtttta gtaggaattt ggaatattaa gtttgataat tggagcaatt ttgctcctta
     3721 cggttttgga caaatctatg gagctagtac tggtattatg gctggtgcgt ccttgatgtt
     3781 cttcggtttt ttgggatttg aatccatctc tatggctgta gatgaagtca agactcctca
     3841 aaaaaatatt cctagaggga ttgtcttatc gctttctatc gtaaccattc tctatgcctt
     3901 ggtgaccctt gttttgactg gtgtggttca ctatagtcat ttaaatgtcg atgatgccgt
     3961 tgcctttgcc cttcgtagtg ttgggattag ttgggcagcc aactatgtgt cattagtggc
     4021 tatcttgacc ttgattacag tttgtatttc gatgacctat gccctatcgc gtatgattta
     4081 cagtttagca cgtgacggct tagtgcctgc tgcctttaag gaactgacga agactagcaa
     4141 ggtaccaaag aatgctacta tcttgacagg tctagcttca gcagtagcag caggaatgtt
     4201 cccactagcc agtattgcag cctttttaaa tatttgtacc ttagcctact tgatcatgct
     4261 ggcttatggt ctgattcgct tacggaaaga aaaaggaatg cccaaagctg gggaatttaa
     4321 aacaccattg gtacctctgt taccaatttt atcgatcatc atttgtttat ccttcatgtt
     4381 acagtataat atgaacacct ggattgcttt cctagttgcg ttgttagtag gaagtattat
     4441 ttacttcact tatggctata agcattctac catagaagaa taaagtgaaa gtctattgag
     4501 ttgttgacac tcagtagatt tttatatgtg aaagaaattt caaatttttt ctaaaaatat
     4561 cttgacagat taaattttca ggagtagaat atttactagt taattaaatg gttaaggagt
     4621 tgttcatgaa gaaacaaaat ttatttttag tcctgttaag tgtctttctt ttgtgcttgg
     4681 gggcttgtgg tcaaaaggaa agtcagacag gaaaggggat gaaaattgtg accagttttt
     4741 atcctatcta cgctatggtt aaggaagtat ctggtgactt gaatgatgtt cggatgattc
     4801 agtcaagtag tggtattcac tcctttgaac cttcggcaaa tgatatcgca gccatctatg
     4861 attcagatgt ctttgtttac cattctcata cactcgaatc ttgggcagga agtctggatc
     4921 caaatctaaa aaaatctaaa gtgaaggttt tagaggcttc agagggaatg actttagacc
     4981 gcgtccctgg actagaggat gtggaagcag gggatggagt tgatgaaaaa acgctctatg
     5041 accctcacac atggctagat cctgaaaaag ctggagaaga agcccaaatt atcgctgata
     5101 aactttcaga ggtggatagt gagcataaag agacttatca aaaaaatgcg caagccttta
     5161 tcaaaaaagc tcaggaattg actaagaaat tccaaccaaa atttgaaaaa gcgactcaga
     5221 aaacatttgt aacacaacac acagcctttt cttatctagc gaagagattt gggcttaatc
     5281 aacttggtat tgcaggtatc tctcctgaac aagaaccaag tccacgacaa ctaacagaaa
     5341 ttcaggaatt tgttaagacc tataaggtta aaacgatttt tacagaaaat aacgcttctt
     5401 caaaagtagc tgaaactctt gtcaaatcaa caggtgtggg tcttaaaact ctgaatcctt
     5461 tagagtcaga cccacaaaat gacaagacct atttagaaaa tcttgaagaa aatatgagta
     5521 ttctagcaga agaattaaag tgaggaaaga atgaaaatca ataaaaaata tctagcaggt
     5581 tcagtggcag tccttgccct aagtgtttgt tcctatgaac ttggtcgtca ccaagctggt
     5641 caggttaaga aagagtctaa tcgagttgct tatatagatg gtgatcaggc tggtcaaaag
     5701 gcagaaaatt tgacaccaga tgaagtcagt aagagagagg ggatcaacgc cgaacaaatt
     5761 gttatcaaga ttacggatca aggttatgtg acctctcatg gagaccatta tcattactat
     5821 aatggcaagg ttccttatga tgccatcatc agtgaagaac ttctcatgaa agatccgaat
     5881 tatcagttga aggattcaga cattgtcaat gaaatcaagg gtggctatgt cattaaggta
     5941 aacggtaaat actatgttta ccttaaggat gcagctcatg cggataatat tcggacaaaa
     6001 gaagagatta aacgtcagaa gcaggaacgc agtcataatc ataactcaag agcagataat
     6061 gctgttgctg cagccagagc ccaaggacgt tatacaacgg atgatgggta tatcttcaat
     6121 gcatctgata tcattgagga cacgggtgat gcttatatcg ttcctcacgg cgaccattac
     6181 cattacattc ctaagaatga gttatcagct agcgagttag ctgctgcaga agcctattgg
     6241 aatgggaagc agggatctcg tccttcttca agttctagtc ataatgcaaa tccagctcaa
     6301 ccaagattgt cagagaacca caatctgact gtcactccaa cttatcatca aaatcaaggg
     6361 gaaaacattt caagcctttt acgtgaattg tatgctaaac ccttatcaga acgccatgtg
     6421 gaatctgatg gccttatttt cgacccagcg caaatcacaa gtcgaaccgc cagaggtgta
     6481 gctgtccctc atggtaacca ttaccacttt atcccttatg aacaaatgtc tgaattggaa
     6541 aaacgaattg ctcgtattat tccccttcgt tatcgttcaa accattgggt accagattca
     6601 agaccagaac aaccaagtcc acaaccgact ccggaaccta gtccaagtc
//
LOCUS       DAVKJU010000085         6641 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVKJU010000085 DAVKJU010000000
VERSION     DAVKJU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.085"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            58..921
                     /locus_tag="VW191_001872"
     CDS             58..921
                     /locus_tag="VW191_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9624592.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKSIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            1304..1924
                     /locus_tag="VW191_001873"
     CDS             1304..1924
                     /locus_tag="VW191_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624593.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2010..2453
                     /gene="tsaE"
                     /locus_tag="VW191_001874"
     CDS             2010..2453
                     /gene="tsaE"
                     /locus_tag="VW191_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU9624594.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2443..2961
                     /locus_tag="VW191_001875"
     CDS             2443..2961
                     /locus_tag="VW191_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624595.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            2969..3985
                     /locus_tag="VW191_001876"
     CDS             2969..3985
                     /locus_tag="VW191_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEU9624596.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSIGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4070..5326
                     /locus_tag="VW191_001877"
     CDS             4070..5326
                     /locus_tag="VW191_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU9624597.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFEGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5381..6547
                     /gene="recA"
                     /locus_tag="VW191_001878"
     CDS             5381..6547
                     /gene="recA"
                     /locus_tag="VW191_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU9624598.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
BASE COUNT         2112 a         1085 c         1496 g         1948 t
ORIGIN      
        1 ttttgaacta aaaactttat aatgaaatta agcaaatagg aaggtttatt atctttaatg
       61 aatacactcg cagagaaatt cagattgaaa agaaaagagt tgagactctc ccaacaaact
      121 cttgcagaag gaatttgtga acaaagccag attagtaaaa ttgaaagagg tcattttatt
      181 ccctccgcag acttgttatt caaactctct caacgacttg aagtcccttt ggattacttt
      241 ttcaatgaac aaatcgaagt taagtctaac ctctctaatt tcaaacatct atctgctcgc
      301 ctattagatg acagaaatta tgacgattta gaatatcttt ataaaataga ggtagaacga
      361 agcacttttc taacattaga agaccgaact taccttgaat ggattaaatc tataattgac
      421 ttctatcaat atgacagtaa gtgtgaggca atttcgtcat tggaaaatat attattaaaa
      481 gtctcttcaa atactctgat ttatttaaag gcgttgaata ctctatctaa tttctattcc
      541 ttagtgggtc gtgaacaaga atatgaggca aactactctc atttaataga gttgtatcag
      601 acaaaaaatt tggaccatca agagttttta tttggctaca tcagagttcg ttacaactac
      661 gctcactacc tagtatcaaa ggaaaaatat aatgaagcca tccaagaagc tcttgaaacg
      721 attgaactct gtaaacaaag acagacaagc taccaattgg ctcctctact tattcttgta
      781 ggaaatgctg gagccaaatt tctagacaga gaacaagtca aaaattatta tatagaagca
      841 agagagttat gtaagattta taacaatcct ttaatgttga tgaagataga aaattatttg
      901 aaggaactag atactgttta gttaatcttg acattatagt ttttctgaaa cgttaaataa
      961 cctgtaaggc tgatagttag attaatacta tcagccttat aattatgtga ctctgagtcc
     1021 gtctcgctcc gtgaggtgcg agacaaaaca accacccgtt tgacggattt tcatgatttc
     1081 aaatttatat tcttcgaaaa tctcttcaaa tcacgtcagc tctatctgca atctcaaaac
     1141 actgttttga gcaacctgtg gctagctacc tagtttgatc tttgattttc attgagtatt
     1201 agtctctagt gtttgtttag cgtcttaaag agacatttga gaagattaga aacagttctc
     1261 tcttttctcc tagtataatg attggtagga agggagagag aagatgaaat caatgagaat
     1321 cttatttttg ttagctttaa ttcaaatcag tttgagtagc tgtttcctat ggaaggaatg
     1381 catcttgtcc tttaaacaaa gtacagcttt tttcatcgga agcatggttt tcgtttcagg
     1441 aatctgtgct ggagtaaatt atctttatac ccgtaagcaa gaagtccata gtgtcctagc
     1501 cagtaagaag tcggtgaagc ttttttacag tatgttactc ttaattaatt tgttaggagc
     1561 tgttcttgtt ttgtcagata acttgttcat caaaaatacg ctgcagcaag aattagttga
     1621 ctttttattg ccatccttct ttttcctatt tgggctagat ttgctgattt ttttaccctt
     1681 gaaaaaatac gtgcgcgatt ttcttgctat gctggacaga aaaaagacag tgttggtgac
     1741 tattttagca acacttcttt tcttaagaaa tccaatgacc attgtctcac ttctgattta
     1801 tattggactg ggcttgtttt ttgcagccta tcttgtccca aattcggtta agaaggaagt
     1861 ttccttttat ggtcatattt tccgagatct tgtattggtc attgttacgc tcattttctt
     1921 ttagagaaag cctgtttttt atggatgtta tgatgataag tttagtagga aaatagacac
     1981 aaaagaaaag tttttggtat aataagagta tgtacacaaa aaatgaagaa gagttgcaag
     2041 ccttagggga gcgtttgggc catctattag caaagaatga tgttttaatc ttaactggag
     2101 aactgggtgc aggtaaaacg acctttacta aaggacttgc aaaaggatta cagatttctc
     2161 aaatgattaa aagtcccacc tatactatcg tgagagagta tgaaggtcga cttccacttt
     2221 atcacctaga tgtttatcgt attgaaggag atgctgattc tatcgacttg gatgagttta
     2281 tctttggtgg cggcgtgact gttattgagt ggggaaatct cttaggagat gccttgccag
     2341 atgcttattt agaattggaa attctaaaag aagcagatgg acgccgttta aattttcagg
     2401 caaagggttt gcgtgctgag aaattgttag aggagcttca atatggagta tgaattgctc
     2461 attagggaag cagagcccaa agatgcagct gaattagtgg cctttttaaa tcgtgtgagt
     2521 ttggagacag actttaccag cctagacgga gatggtattc tcttgactag tgaggagatg
     2581 gaaatattcc tcaacaagca agctagttcg gacaatcaga taaccttact tgcattctta
     2641 aatggcaaaa ttgctggtat tgtaaatatt acagctgatc agcgtaagag agtccgtcat
     2701 attggagatc tcttcattgt gattggaaaa agatattgga ataatggctt gggaagtttg
     2761 ttgctagaag aagcgataga gtgggcacaa gcaagtggca ttctgcgtcg tctccaactg
     2821 actgtccaaa ctcgtaatca agcagcagtc catctttatc aaaagcatgg ctttgtcatt
     2881 gaaggtagcc aagagcgtgg ggcatatata gaagaaggga aatttatcga tgtttacttg
     2941 atgggtaaac tgataggtta gtagaaatat ggttaaaaaa attattggaa tggtgctagc
     3001 tttactttct gtaactgtag taggagtagg tgtttttgct tatactattt atcaacaagg
     3061 gacagaaacc ttagctaaaa cctataaaaa aatcggtgaa gaaaccaagg ttattgaagc
     3121 gactgaacct ctaaccattc tgttaatggg agtggacacc ggaaatgttg aacgaactga
     3181 aacttgggtc ggtagaagtg atagcatgat cttgatgaca gtgaatccta aaacgaaaaa
     3241 aacaacaatg atgagtttag agcgggatat tctgacgcgc attgaatcag ggaatggtca
     3301 ggctcatgaa gcgaaactga actcagcata tgcagatggt ggagcagagc ttgctataga
     3361 aaccattcaa aaaatgatga atatccatat tgatcgctat gtgatggtca atatgagagg
     3421 gttgcaaaaa ttagtggatg cagtaggagg tattacagtc aataatatcc taggtttccc
     3481 aatttctatc agtgaccaag aagaatttaa taccatttct atcggtgttg gggagcaaca
     3541 tattggggga gaagaagccc tagtctatgc acgaatgcgt taccaagatc ctgaggggga
     3601 ttatggtcgt caaaaacgtc aacgtgaagt tattcaaaaa gtcatggaaa aagctctcag
     3661 tttaaatagc attggtcatt atcaagagat tctaaaagct ttgagtgaca atatgcagac
     3721 caatattgat ttgtctgcaa aaagtatccc taacttgcta ggctataaag attcatttaa
     3781 aaccattgaa actcagcagt tgcagggtga aggagagata cttcaaggtg tttcttacca
     3841 gattgtttcg agagcacata tgttggaaat gcaaaatata ctccgacgtt ctttgggaca
     3901 agaagaagtt actcagcttg aaaccaatgc ggttttattt gaagatttat ttggcagagc
     3961 acctgttggt gatgaagata attaactttt gatacaaata aaaaaatcaa tcgtaggaaa
     4021 attcctgcga ttttttcaaa aaaatacgaa tagataggta ggaggaaaca tgaaagcaga
     4081 aatcattgct gttggaacag agattttgac aggacagatt gtcaacacca atgcccagtt
     4141 tttgtcggaa aaactagctg agattggggt agatgtatat tttcagacgg ctgtaggaga
     4201 caatgaagtt cgtctcttgt ctttgcttga gattgccagt caacgtagca gtctggtgat
     4261 tttgacaggc ggtttggggc caactgagga cgacctaacc aaacaaaccc tagctaaatt
     4321 tttagggaaa gcattagtct ttgatcctca ggctcaggag aagttggata tcttttttgc
     4381 cctgcgacca gactatgccc gaacaccgaa taacgaaaga caagctcaaa ttgtagaagg
     4441 agcgattcca ctgccaaacg aaacaggact ggctgttgga ggaaaattag aagtagacgg
     4501 agtgacctat gtcgtccttc caggtccgcc aagtgaattg aaacccatgg tcttaaacca
     4561 acttctaccc aagttgatga cagggagcaa gctgtattcc cgagttcttc gtttctttgg
     4621 gattggcgag agccagttgg ttacgatttt ggctgattta attgataatc agatcgatcc
     4681 taccttggcc ccttatgcca agacaggaga agtcactcta cgtctgtcaa caaaggctag
     4741 cagtcaagaa gaggcgaatc aagcgctgga tatcttggaa aatcaaatct tggactgcca
     4801 gactttcgaa ggaatttctt tacgagactt ttgctatggt tatggggaag aaactagttt
     4861 agccagcatt gtggtagaag aactgaaaag gcaagggaaa accatcgcgg ctgcagagag
     4921 tttgacggca ggtcttttcc aagctaccgt ggcgaatttt tctggagttt caagtatatt
     4981 tgagggtggt tttgtgacct atagcttgga ggaaaaatca aggatgttgg atattcctgc
     5041 caagaatttg gaagaacatg gtgtggtgtc tgaatttaca gctcagaaga tggctgagca
     5101 ggcacgaagc aagacccagt ctgattttgg aattagtttg actggagtgg caggaccaga
     5161 tagcctagaa ggacacccag ttgggacagt cttcataggc ttggcgcaag atcaaggaac
     5221 tgaggttatc aaggttaata ttggaggcag aagccgagca gatgtacgtc acattgcggt
     5281 tatgcatgcc tttaacctag ttcgcaaggc tttattaagt gactaacttt tgatataata
     5341 gtagataggt ctgaggatca ttagaatgta ggagaataga atggcgaaaa aaccaaaaaa
     5401 attagaagaa atttcaaaaa aatttggggc agaacgtgaa aaggccttga atgacgctct
     5461 taaattgatt gagaaagact ttggtaaagg atcaatcatg cgtttgggtg aacgtgcgga
     5521 gcaaaaggtg caagtgatga gctcaggttc tttagctctt gacattgccc ttggctcagg
     5581 tggttatcct aagggacgta tcatcgaaat ctatggccca gagtcatctg gtaagacaac
     5641 ggttgccctt catgcagttg cacaagcgca aaaagaaggt gggattgctg cctttatcga
     5701 tgcggaacat gcccttgatc cagcttatgc tgcggccctt ggtgtcaata ttgacgaatt
     5761 gctcttgtct caaccagact caggagagca aggtcttgag attgcgggaa aattgattga
     5821 ctcaggtgca gttgatcttg tcgtagtcga ctcagttgct gcccttgttc ctcgtgcgga
     5881 aattgatgga gatatcggag atagccatgt tggtttgcag gctcgtatga tgagccaggc
     5941 catgcgtaaa cttggcgcct ctatcaataa aaccaaaaca attgccattt ttatcaacca
     6001 attgcgtgaa aaagttggag tgatgtttgg aaatccagaa acaacaccgg gcggacgtgc
     6061 tttgaaattc tatgcttcag tccgcttgga tgttcgtggt aatacacaaa ttaagggaac
     6121 tggtgatcaa aaagaaacca atgtcggtaa agaaactaag attaaggttg taaaaaataa
     6181 ggtagctcca ccgtttaagg aagccgtagt tgaaattatg tacggagaag gaatttctaa
     6241 gactggtgag cttttgaaga ttgcaagcga tttggatatt atcaaaaaag caggggcttg
     6301 gtattcttac aaagatgaaa aaattgggca aggttctgag aatgctaaga aatacttggc
     6361 agagcaccca gaaatctttg atgaaattga taagcaagtc cgttctaaat ttggcttgat
     6421 tgatggagaa gaagtttcag aacaagatac tgaaaacaaa aaagatgagc caaagaaaga
     6481 agaagcagtg aatgaagaag ttccgcttga cttaggcgat gaacttgaaa tcgaaattga
     6541 agaataagct gttaaagcag tggagaaatc cgctactttt tcgatttttg attcaagttt
     6601 ttagattata tatagtagct tgaaataaga tatgaacaac t
//
LOCUS       DAVKJU010000086         6151 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVKJU010000086 DAVKJU010000000
VERSION     DAVKJU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.086"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(112..387)
                     /locus_tag="VW191_001879"
     CDS             complement(112..387)
                     /locus_tag="VW191_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU9624599.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(494..1333)
                     /locus_tag="VW191_001880"
     CDS             complement(494..1333)
                     /locus_tag="VW191_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU9624600.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1475..2323
                     /locus_tag="VW191_001881"
     CDS             1475..2323
                     /locus_tag="VW191_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9624601.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2416..3333)
                     /locus_tag="VW191_001882"
     CDS             complement(2416..3333)
                     /locus_tag="VW191_001882"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU9624602.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3771..3976
                     /locus_tag="VW191_001883"
                     /pseudo
     CDS             3771..3976
                     /locus_tag="VW191_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1980 domain-containing protein"
     gene            complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VW191_001884"
     CDS             complement(4287..4580)
                     /gene="rpmA"
                     /locus_tag="VW191_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU9624603.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4597..4941)
                     /locus_tag="VW191_001885"
     CDS             complement(4597..4941)
                     /locus_tag="VW191_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEU9624604.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     LNSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VW191_001886"
     CDS             complement(4957..5271)
                     /gene="rplU"
                     /locus_tag="VW191_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU9624605.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5490..6035)
                     /locus_tag="VW191_001887"
     CDS             complement(5490..6035)
                     /locus_tag="VW191_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEU9624606.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLIAFIMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVPIMMSIFYLSMLFA"
BASE COUNT         1888 a         1260 c         1015 g         1988 t
ORIGIN      
        1 aatattttcg gaacattcaa ctttttaact ctatttatta ctaaatttca taattaaaaa
       61 gcctactgac caagctagaa agcttgatac aataggcttt ttaaagactg attatttaac
      121 agcgtcttta agagctttac cagctttgaa tgctggtact ttagaagctg caattgtcat
      181 ttctttacca gtttgtgggt tgcgaccttt acgttctgcg cgctcacgaa cttcaaagtt
      241 accaaaaccg atcaactgaa ctttttcacc agctgcaaga tagtcagcta ctgctgcaaa
      301 tacagcttca actgctgctg ctgagtcttt cttagtcaat tctgtagctt ctgctacttt
      361 agcgatcaaa tcttgtttgt ttgccatgtt aatgattcct ccaaataatt tctaattaac
      421 aaatataatc atatcctaaa atccctttta ggtcaagtta aaaacgctat ttcttcccat
      481 tttatttatt tttttaggag tggtaacgta tcaaaatagc ccaagcgttc tcacccgtgt
      541 gagtttgaat aatggaaccc gtttccaaaa cagaaattgg cttttcaaca taagcttgta
      601 agctttcttt catctctttt gcccaatcat cactaccaga atatgaaatt ccaatctctg
      661 ctacagcacg ttcagaaagc gatgttatca actcatctaa ccatttttta aatgttttag
      721 ttccacgacc tttaaccatt ggctgcaatt catggtcttt catttgcatg acagcacgga
      781 tattgagaag agagctcaac aagccagtta cacggctaat tcgtccacct ttgacaagat
      841 tttccaaagt cgaaacacca atataaagct ctgtatggtt tttaacctct tctacatgag
      901 ataaaattgc ctccatatct ttaccttctt gagctaactt cgcagcctca acaacttgga
      961 atttcagggc ttggtcagtg aaggaactat caacaacagt cacatctgca gtagataggc
     1021 tagcaccttg gcgtgctgct tctaccgtac ccgaaagagc atgggacata tgaatagcaa
     1081 gaatctggcc accatctttg cataggtctt caaaaatctc agcaaagaca cctacaggtg
     1141 gctgacttgt tttcggaaga ttcttacttt cttgcatcaa ctgaagaaat ttaccttctt
     1201 ctttcaaatc cgcatcagaa taaacaacat tatcaatcat tacagataat ggaacaattg
     1261 taatatctaa ttgctttact agttcaggtt caatagtaac agatgaatcg gttacaatct
     1321 taatttttgt catagtatca atctttctat tttaggattc agattggttt ccttacttct
     1381 aattatatca aaaaaaagat taaaaatcct aatggagtca atcaaatttt ccgtaaaatt
     1441 tgatataatc aacttataag aaaagaggtg tcctatgatt aaaaaaattt accccatttt
     1501 taccatttta ctaggtgctg ctatttatgc ttttggactg acttattttg tagttcccca
     1561 tcatctcttt gaaggagggg cgacaggcat taccctcatc accttttatc tttttaaaat
     1621 ccctgtttcc ctcatgaacc tgctgattaa tattcccctt ttcatcctag cttggaagat
     1681 ttttggagcc aaatccctct attctagttt actaggaacc ttagctttgt ccggctggtt
     1741 agcttttttt gagcatattc cccttcatat tgatcttcaa ggtgatttac taatcacagc
     1801 ccttatagcg ggaatcctat tgggaattgg ccttggaatt atttttaatg ctggaggtac
     1861 aactggcgga actgatattc tagctcgtat tctcaacaaa tacactcata tatccatagg
     1921 aaaactgctc tttatcttag atttttgtat tctcatgttg attctcctaa tcttcaagga
     1981 tttgagattg gtttcctaca cgcttttgtt tgattttatt gtttctcgtg ttattgattt
     2041 gattggtgaa ggaggatatg ccggcaaagg ctttatgatt atcacaaaac gtcctgacca
     2101 acttgctaag gcgattaatg atgacctcgg aagaggtgtt acttttattt ctggtcaagg
     2161 ctactatagt aaagaaaatt tgaaaatcat ctactgtatt gtcggaagaa atgaaattgt
     2221 gaaaacgaag gaaatgattc atcgaatcga tcctcaagcc tttataacta ttacagaagc
     2281 ccatgaaatc ctaggagaag gcttcacctt tgaaaaagaa taaaaagagg taatgtcgtg
     2341 acctcaaaag ttagactaaa tcatctatct tttgggttac agacaacctc ttttttgttt
     2401 tatttactca agctcttaag accaattccg agttacttct tcatcagcct ttaactgatc
     2461 cactaattgg tcaactgagt caaatttggt catatctcga atgcgatcaa gccaataaac
     2521 catgacggtt tccccataaa tatcttgatt aaaatcaaaa atattgactt caaaacgtgc
     2581 ttcttctcca tcaaaggtca catttttccc gacactagcc atagcacgat acttctgtct
     2641 ttgaatctca acatcaacga cataaacgcc atctgctggc atataagtac ggtctaaaag
     2701 cactaaattc gctgtcggat aaccaattgt acgaccacga gcattaccat gaactaccat
     2761 acctcttgat ggaagcggtg cccccaaaag ttttcctgct tctttcacat ttccatctaa
     2821 aatagcttga cggatacgag ttgaactaat ctttcctttc tcatcttcta caggtggaac
     2881 aatgataact tctccatcaa agtaatcctt taaatcttct gctgtttttt tgtcagaacc
     2941 aaatgtataa tcaaaacctg caacaataat tttggcattc atagccttga tataagttgc
     3001 aaagaattct tgtgcagtga gactagcgaa ttgactacta aaatcaagga gatataattc
     3061 ttctacacct tcgcgcttta attttctttc acgttcagca gggttcaaaa tatgcaaaaa
     3121 caaatctgga tgataaggct ctaaagcgat ctttggagat tcattaaagg tcataacgac
     3181 gataggcaat aaatcctttc tcgcagcctt gttggcaaca cgaaataatt cttgatgccc
     3241 cttatgtatg ccatcaaaat agccgagaac aacgactgaa tcagatggtg tgccaatatc
     3301 tttttggttt tttataggaa tagtaataat cataaaataa ttatatcata gcgatagcta
     3361 tttctggaac agaaaatctg aaatgttgtt tttttcacat gaagtgtacc tattttcaaa
     3421 aagcacttta ttctatcgtt gcttaactat gaactttgca atattcttct caaaaacttg
     3481 taggacatct tcaaaatttt gcaaggagtg attagacttg ttcggtaacc ataaagtgtc
     3541 atactatgct tatgtatgaa aaagcaatgc aactaactcc tgagaacttt aaattactaa
     3601 ttggtgccga aaaggtagaa tttagaatca aggtacacct atggctgtaa aatttacaaa
     3661 atgagacaac ttgagcaaga tgtttgaaga atttcctaaa ctccctgatt tgaagcaagt
     3721 cactttccct gatgacaaag aaaaaagcca aaacagcaaa gaaaaactag atgactgctt
     3781 tccaacaact cccatctagt gtgcttcaga ctgggctatt tttctctcca tctgttaact
     3841 tggattctca gaccgtttca gctaaagaat atcttttccc ttatcagaag gaacggctca
     3901 agccattcag acaagtgaag ggacgacaag ccaatatttg aaaccagata ccagttctta
     3961 tagtcaattg aaataaaatc tgaagaaatc gagtaggaaa ctcatatcaa tttttaacag
     4021 tgttctattc cagattcata ctcaatgaaa attaaagtgc aaactaggaa gttagccgca
     4081 ggctgtactt gagtacggca aggtgaaatt gacgtggttt gaatttgatt ttcgaagagt
     4141 atcaatccac tatacatatt aaaaagtatg ggtatcaaaa taaaaaaaca ggctctccga
     4201 aaactcggaa agccttattt taagctgttt ctagcttcct cacctttaca tttcaaggtt
     4261 cgggataaaa ggttcaatga acctttttat tttgcgattg ggtaaacaga aacttgtttt
     4321 ttatcgcgtc ctttacgttc aaagcgtact acgccttcaa ctttagcgaa caaagtatcg
     4381 tctccaccac gaccaacgtt tacacctgga tagatgtgtg taccacgttg acggtaaagg
     4441 attgatccac ctgttacagt ttgtccgtca gctgctttag ctccaagacg ttttgcttgt
     4501 gaatcacgtc cgtttgatgt agaacctcca ccttttttgt gggcgaaaag ttgcaagttg
     4561 ttaagagtca tttttaacat aatgttttcc tccgtgttag ttttctgtga taactctggt
     4621 ttggacgaac tcagaagagt tctccgataa gtttgccata cctaagaaaa atgattcaaa
     4681 gaataactgg gtcatttctc tctggtgtga aggaagatct tttggtattt caaccatcag
     4741 atagccacct tcatcttcgt ttaattctag gattggttca tagcctgcaa atttctcaat
     4801 agaattgaga aagttaatgg caagcgtaga aaccgatgca cacacgacat ctaagccgta
     4861 ttcgccactc tcggcgtgtc cagtaatttc cgcactcctc agctcgccat cttcggctct
     4921 ctcaaagact gcttgtatca tgtgttctcc ttaaaattaa gcgttaattg cgttgatgac
     4981 aacttttgta tatggttgac ggtgaccttg tttacggtgg ctaccttttt taggtttgta
     5041 cttgtaagta acaactttct tttgttttcc ttgtttttca acagttccaa ctacagtagc
     5101 tccagcaaca agtggagttc cgacaacagt gttttcacca ccaacaagaa caacttcgtt
     5161 aaaagtaact tcttgaccag cttcaacgtt caatttttca atgtaaactg cttgaccaac
     5221 ttcaacttta acttgttttc cgccagtttt gataattgcg tatgtgctca ttatgcacct
     5281 cctatgattt ttatgggttt ccccgatttt tttgtgaaga ctcgcctagc atcgtgggac
     5341 aaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 caagcttttt cttttacttt tttctgccac taggcaaaaa gcatactgag ataaaaaatg
     5521 ctcatcataa taggaacgcc aaggacaaaa ctactaagca ggctaacaaa tatgaaccct
     5581 tcacgcaaaa aaggagtgtg cttagttcgg aaataatctc caaaagctag cattgttcgt
     5641 ttgatattcc ctgtcataaa ggcattattg taggcaatac ccgaaacctc accaaaagca
     5701 gttgtcacca gtcccataca gaaggccaag ggcggcacta gatagatatt atctacagtt
     5761 tgtggcacaa aaccaataat tattgatagg attgctaaag gaatcaagga cagaatagat
     5821 tttttcacaa ttctcaattt ttccttataa atcgttaata aaaagactcc cattataaac
     5881 gctattaagg ttaaaagctt atccctaaca tccgaaacat tattttgtat gagctctacc
     5941 gaaagaaaga caacatttcc agcttgtcca gctacaaggg tattccctcg aacaataaaa
     6001 gtgtaagcat ccacatatcc agcacaaaac gtcaaaaaag tgctaacctt ttagactgac
     6061 gtggtatttt tcttataggt aataacctca ttttccctcc cattgtattt tctcttagaa
     6121 atattgtacc atttctttct aaaaaatcgt a
//
LOCUS       DAVKJU010000087         6034 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVKJU010000087 DAVKJU010000000
VERSION     DAVKJU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.087"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            150..458
                     /locus_tag="VW191_001888"
                     /pseudo
     CDS             150..458
                     /locus_tag="VW191_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            637..1296
                     /locus_tag="VW191_001889"
     CDS             637..1296
                     /locus_tag="VW191_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEU9624607.1"
                     /translation="MKNLTKIKFKENGEFNHFSGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRVTEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1321..2388
                     /locus_tag="VW191_001890"
     CDS             1321..2388
                     /locus_tag="VW191_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU9624608.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVQ"
     gene            2491..3501
                     /locus_tag="VW191_001891"
     CDS             2491..3501
                     /locus_tag="VW191_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624609.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALTRALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3513..5204
                     /locus_tag="VW191_001892"
     CDS             3513..5204
                     /locus_tag="VW191_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9624610.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5207..5917
                     /locus_tag="VW191_001893"
     CDS             5207..5917
                     /locus_tag="VW191_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU9624611.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2028 a          839 c         1154 g         2013 t
ORIGIN      
        1 tcgcttacca atatttgcta aactttcaga aaaaaagttg gagtatatga tgctaggtga
       61 ctgcgtcatg ttagtaaacg aaatggaaat aactgatcat agagtagata acttatttga
      121 aaaagggaaa aatgagataa aagattcaag tagtagattt agcaatagga catataaaag
      181 ctttagaaaa agtatctgaa aaaacagatg tttatatcta taacctaggc tcgggagaag
      241 gcactagtgt attacaactt gtaaatacat ttgaaagtgt taataagatc cctattcctt
      301 ataaaatagt tccaagacgt tcaggagacg ttgcaactta ttatgcaaat gcagacaaag
      361 catataagga attaaattgg aggacaacaa aatcgattga agacatgtgt agagatacat
      421 ggaattggca agcaaaaaat cccaatgact ataattgatg atactgtcta tatgagtgct
      481 gtattttgga aaaattactt aaccttttaa tgaaatgcat atttaatgaa agataaataa
      541 aaaaaagttt gacagcgctt gacaaaggaa acggtttcat tgtataatgt aaacatagtg
      601 acaccgatgt caaatataaa aaaagaaggt ctttatatga aaaacttaac aaaaattaag
      661 ttcaaagaga atggggaatt taatcatttc tctgggaata cagttgtagc aaatctttat
      721 actaaacaag atttgatgga agttgttgat attattcaat cacgttatag agaattacca
      781 tttatcgata agtttacttt aactccaagg aattctattc acatgactgt aattgaattg
      841 ttgtgccatg aaaatcgtga aacggagttt tggagcagta atcttcctct agatacacct
      901 ttacaggaaa tacatgatta ctttgctaaa cagcttgaaa tttttccatt gttagatgaa
      961 gaaattcata tgcgtgtaac tgaaatggga aaacaaaaca tactagttga acctgcagat
     1021 gaagcttctg caaagagatt agaagaaatc cgtacttatg tctcagaaaa agcaggtgtt
     1081 tgtttcccta atcatgatag atatcaattc catatttcaa ttgggtatct tcggattcct
     1141 ctaaccgaag aggaggaaga agaatttact aaagtcagag cagaattaac tgaaatttta
     1201 ttagagaaga ttccaactat tactgtaaac cgtattgact atactgtatt tgaagatatg
     1261 agacaatttg ttccatatca cgaaaaattt aaataaaata ataaaaaagg agaacttatc
     1321 atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg gagttgctct tactacactt
     1381 gctgcttgct ctggaggttc aaattctacg actgcttctt catctgaaga aaaagctgat
     1441 aaaagtcaag aattagtaat ctattcgaac tcagtctcaa atggtcgtgg tgattggtta
     1501 actgctaaag caaaagaagc tggttttaat ataaaaatgg ttgatattgc tggcgctcaa
     1561 ttagcagacc gtgttattgc tgagaagaat aatgcagttg cagatatggt atttggaatt
     1621 ggtgctgttg actcaaataa aattagagat caaaaattac tagtacagta caagcctaaa
     1681 tggttagata aaattgatca atctttatca gataaagata attattataa tcctgtgatt
     1741 gttcaaccat tagttttaat tggggcgcct gatgtaaaag aaatgcctaa agattggact
     1801 gaattaggta gtaagtataa aggtaaatat tcaatttatg gtcttcaagg tggtacagga
     1861 cgggcaattc tagcaagtat cttagttcgt taccttgatg aaaaaggaga tttaggcgta
     1921 tcagaaaaag gatgggaagt tgcaaaagaa tactttgcaa atgcctatac tcttcaaaaa
     1981 ggtgaaagct cagttgttaa agcgttagat aaagaaagcc caattcaata cggtatgatc
     2041 tggggttctg gagcgttaat cggacaaaaa gaacaaaatg ttgaattcaa agttatgact
     2101 cctgagattg gtgtaccatt tgtaactgaa caaactatgg ttttaagcac tagtaaaaaa
     2161 caagcgttag ctaaagaatt tattgattgg tttggtcaat cagaaattca agtagaatat
     2221 agtaaaaaat ttggatctat tcctgcaaat aaagatgccc tcaaagagct accagaagat
     2281 actaagaaat ttgttgatca agtgaaacca caaaatattg actgggaagc tgttggaaag
     2341 catttggatg aatgggtaga aaaagctgaa ttagaatacg tacaataaga aataagagtt
     2401 attatttatg tccaagtctc tctattattt ctaaatttat ggaagaaata aagagaggct
     2461 tttggtttag taatagaggg agttagtgag atgattaaat ttgataatat tcaaattaaa
     2521 tatggtgatt ttgttgcaat tgataatctg aatttagata tacatgaagg ggaattcttt
     2581 acatttcttg ggccttcagg atgtggtaaa tcaactactt tgagagcatt ggtaggtttt
     2641 ttagatccat catcaggaag tattgaagtt aatggaacag atgtcactca tttggaacct
     2701 gaaaagcgtg gaattggtat tgtatttcaa tcttatgcgc tatttccaac tatgactgtt
     2761 tttgataata ttgcatttgg tttaaaagtt aagaaggtag ctccagatgt tattaaagct
     2821 aaagtatcag cagtggcagc aaaaattaag atctctgatc aacagttaca gcgtaatgta
     2881 tcagaattat ctgggggtca acaacaaagg gtagcattga ctcgtgctct ggttcttgaa
     2941 cctaaaattc tttgtctaga tgaaccattg tcaaaccttg acgcaaaatt acgtgtagat
     3001 ttgagaaaag agttgaaaag acttcaaaaa gagttaggta ttactacttt atatgttact
     3061 catgatcaag aggaagcctt gactttatct gatagaattg cagtctttaa caatggatac
     3121 atcgaacagg tcggtacacc agtagagatt tatcataatt ctcaaactga atttgtatgt
     3181 gattttattg gagatattaa tgttttgacc gatgaaacag tccacgaagt attactgaaa
     3241 aatacaagcg ttttcttaga agataaaaaa ggatacattc gattagagaa agttcgattc
     3301 aatcgtgaaa ctgaacaaga tttcattcta aaagggacaa ttattgatgt tgagttttct
     3361 ggagttacaa ttcactatac aataaaagtt tctgaaagtc agattcttaa tgtaacaagt
     3421 attgatagtc aggctgctat tagatctgtc ggagaaagtg tggaattatt tatcacacca
     3481 tcagacgttc tgcaatttta aggaggtgca gtatgcgtca taaattaaat ttaaaagatt
     3541 ggcttattcg tttagggtta atctggttct tagtaacatt tattatttat ccaaactttg
     3601 atctagtagt gaatgtattt gtaaaaggag gagaattttc ccttgatgct gtacatcgtg
     3661 ttctaaaatc tcagagggca cttcagagta ttatgaacag ttttaagtta gcattttcac
     3721 ttattattac agttaatgtc gtaggtattc tttgtgttct atttacagag tactttgata
     3781 ttaaaggtgc taaaatttta aaattaggtt atatgacctc tttaatttat ggaggagtgg
     3841 ttttagcgac tggatataaa tttgtctatg gtccttatgg attgattaca aaatttttac
     3901 aaaatgttat cccttcttta gactctaact ggtttattgg gtatggtgca gtcttattca
     3961 ttatgacatt ttcaggaact gctaatcata cattgttttt aacaaataca attcgaagcg
     4021 ttgactatca cactattgag gctgctcgaa atatgggagc aaaaccattt actgttttcc
     4081 gaaaagtagt gttaccaacc ttaattccaa ctctatttgc acttactatt atggtttttc
     4141 ttagtggttt atctgcagta gcagcaccca tgattgttgg tggtaaagaa tttcaaacta
     4201 taaatccaat gattattaca tttgcaggga tggggaattc tcgtgattta gctgccttac
     4261 ttgcaattat tttaggtatt gcaactacaa ttttgcttac tatcatgaat aagatagaaa
     4321 aaggtggaaa ttatatttct atctctaaga ctaaagcgcc tcttaaaaaa caaaaaattg
     4381 cgtctaagcc ttggaatatc attgctcaca ttgtagcata tggattgttc acagttttca
     4441 tgcttccact aatttttata gtattatact catttacaga tccagttgca attcaaacag
     4501 gtaacttaac attatcaaac tttactttag aaaattatcg cttattcttt agtaatagtg
     4561 cggcattctc tccattcttg gtcagcttta tttattctat tattgctgcg acaacagcaa
     4621 caattctcgc agttgtattt gctcgtgttg tcagaaaaca taaatctcgt tttgatttct
     4681 tatttgaata tggtgctcta cttccttggt tactaccaag tacactttta gcagtaagtt
     4741 tattatttac ttttaatcag ccacaatttc ttgtcttgaa tcagattttg gtaggtagtt
     4801 tggtaattct acttattgca tatatagttg taaaaatccc attttcttat agaatggtac
     4861 gtgctatttt atttagtgtt gatgatgaga tggaagatgc agcaagaagt atgggtgctt
     4921 caccttttta tactatgatg aaggttatca ttccatttat tttaccggtt gttctctctg
     4981 ttattgcttt aaactttaac tctttattaa ctgacttcga cttatctgta ttcctttacc
     5041 atcccctagc tcaaccatta ggtattacga ttcgatctgc aggtgatgaa acagcaacat
     5101 ctaatgcaca agctctggta tttgtttata caattgttct gatgattatt tctggaacgg
     5161 tattatactt cacacaaaga ccggggcgta aagtaaggaa ataatcatga cagccactag
     5221 tcttgggtta tcaaatattg aaatagttgt caggattgtt ttatcagtag tcattggtag
     5281 tataattggt ttagagagag ggagcaaatc ccagcctgca ggcatccgaa cttatagtat
     5341 tgtttgtcta gctgcatgtt tgattatgat gacgaatgaa tacgtatctt ataaatttgg
     5401 gacaggagat cctacacgat taggagctca agttatatca ggtgtgggtt ttctaggcgc
     5461 tggaacgatt cttattacag ataaaaagaa aattacaggt ctgacaactg cagcaggcat
     5521 ttgggcttcg gcaggaattg gattagctat tggagtaggt ttttatgagg gagctctttt
     5581 agtagccatt tctgtttggg gtgtgatatc catgttccaa ccactaaaaa aatatctgca
     5641 aaatcgttct aaaatgattg aattgtatat agtagttaaa tctacagaag cctataatcg
     5701 tgtattggtt tattgtgcag aaaatggtat cagaatgacc gattcaagaa cagcgtttgg
     5761 agatgttaat tctgacagaa ttgaatactt tgatgttccg gacaaaaaaa tagcgtcatt
     5821 tattactctg gaattatcag gtagatttga acatcttcga ctgatggaag aaattgctaa
     5881 tattgtaggt gttatttatg tcgaagaaat tagctgaaat ttagtaaaca aagggacaga
     5941 tgtttttgtc cttttgtttt ttagatgttt taatcatcta atttttgagt agatttttgc
     6001 tcagaggttt ttatgaaaga ttataaaatc aatt
//
LOCUS       DAVKJU010000088         5309 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVKJU010000088 DAVKJU010000000
VERSION     DAVKJU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.088"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            171..698
                     /locus_tag="VW191_001894"
     CDS             171..698
                     /locus_tag="VW191_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEU9624612.1"
                     /translation="MEDYCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNT
                     RKAQGRHLEQEGNIKHALIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQ
                     TLMESLSDDDYDFAFDEALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKY
                     ECYSPLNNLELDDFE"
     gene            complement(864..935)
                     /locus_tag="VW191_001895"
     tRNA            complement(864..935)
                     /locus_tag="VW191_001895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(900..902),aa:Arg,seq:cct)
     gene            complement(977..1324)
                     /gene="rplS"
                     /locus_tag="VW191_001896"
     CDS             complement(977..1324)
                     /gene="rplS"
                     /locus_tag="VW191_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU9624613.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(1443..1772)
                     /gene="crcB"
                     /locus_tag="VW191_001897"
     CDS             complement(1443..1772)
                     /gene="crcB"
                     /locus_tag="VW191_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9624614.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(1766..2140)
                     /gene="crcB"
                     /locus_tag="VW191_001898"
     CDS             complement(1766..2140)
                     /gene="crcB"
                     /locus_tag="VW191_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU9624615.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(2137..2403)
                     /locus_tag="VW191_001899"
     CDS             complement(2137..2403)
                     /locus_tag="VW191_001899"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU9624616.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(2519..2962)
                     /locus_tag="VW191_001900"
     CDS             complement(2519..2962)
                     /locus_tag="VW191_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEU9624617.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            3066..4001
                     /locus_tag="VW191_001901"
     CDS             3066..4001
                     /locus_tag="VW191_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU9624618.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            4055..4339
                     /locus_tag="VW191_001902"
     CDS             4055..4339
                     /locus_tag="VW191_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEU9624619.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(4396..4614)
                     /locus_tag="VW191_001903"
     CDS             complement(4396..4614)
                     /locus_tag="VW191_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624620.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETKPTDESGT"
     gene            complement(4611..5309)
                     /locus_tag="VW191_001904"
                     /pseudo
     CDS             complement(4611..5309)
                     /locus_tag="VW191_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         1698 a         1111 c          932 g         1568 t
ORIGIN      
        1 ataatttagc tacttctgga aaaaaagcag aattaattac aagaataatt aataatgtta
       61 acattgacaa tttagatatt ccgttcgaat ttaaactaac aaaagaagca caaaatctta
      121 ttatcgaaca tagtgactat atcaaagcat actatgataa agacataact atggaagatt
      181 attgtaaaga aaaaaacaat atctctttta aagcaacttt tggtgatata aaatggagtc
      241 tcttaaataa acaagctcat aggaatactg tatcaggaga ttttggatgc ttatctaaca
      301 cacgaaaggc tcagggaaga catttggaac aagaaggtaa tattaaacat gctttaatat
      361 attacataga atctttgata attactattt caggattaga aaacaatttt tcagccactg
      421 attatccagt atattatccc gattcgatac ctgactactc actaaaacat attcaaacat
      481 taatggaatc attatctgat gacgattatg attttgcttt tgatgaagca ttatttcgct
      541 tctcaatttt gaatgcaaat cattttttat ctaaggaaga tattgactat ttaagagtta
      601 atttacctcg ttccactgct gaagaaataa acaattactt aaagaaatat gaatgttata
      661 gtcctttaaa taatttagaa cttgacgatt ttgaataaat tgactataca aacatttata
      721 tactcgatat agtctcaatt ttatctgatg attgcccaaa tttttcaata ataaaacgca
      781 taatattatg gagacaatcc cctatattat gcgttctttt aatatcaaag actttttgac
      841 aaacttcttc gatatctaat tactgccccc tgcaggaatc gaacctgcaa ctactcctta
      901 ggagggagtt gttatatcca ttgaactaag ggagctagat aaaaactctg ctgaaaagca
      961 gagtttttta gtcgaattaa cgacggattt ctttgatacg agctgcttta ccttgaagag
     1021 cacgcaagta gtacaatttc gcacgacgta ctttaccgta acgaacaact tcgatttttt
     1081 caacacgtgg agtgtggatt gggaagatac gctcaacacc tacaccgtta gagattttac
     1141 gaactgtgta gttttctgag attccagcac ctttacgtgc gataacaaca ccttcaaaaa
     1201 tctggatacg ttcacggtta ccttcgacaa ctttcgcgtg tacacgaaca gtgtcaccag
     1261 gacggaatga tgggatatct gtacgaagtt gaccttcagt caagctttgg attaatggat
     1321 tcattttatt ctcctatctt tgtcaatctt gaggaaattt cctcagcgga taaactgtat
     1381 ttttgtgcgt ccatcacaca caagatacag tctaccaaat tttcacaaaa aagtaaagaa
     1441 atttatagca aaattcctaa aaaaatcgca accaaaccac ctatgtaagt caaagtcagg
     1501 taagaataaa agaccttctt atcacttaac agtctttgaa gttcgtcatt caaggtcgaa
     1561 aaagttgtta aaccgccaca aaatcctgtt gctagaatag catatacttc cttagactct
     1621 acatgattgt agaatactcc aattaaaaaa caacctaaaa gattagctat gagtgttccg
     1681 agtggcaatt tagaagcttg attgtagcgg gaaaagaaat accgtacgag tgctcccaag
     1741 ccacaggcga ttgcaagata gacgattacc atttcttcct ccctagaaaa taagctaaga
     1801 gtaggcctcc accgatgctc aaaagcaaat acaggactaa actaagataa cgccctgtat
     1861 caagcagctt cacagtatca agcattaggc tggaaaaagt tgttaaacct ccgcaaaaac
     1921 ccgtccccag tgctaaaatc aagcccttac tagtcccctt atagaccaga tagcccttga
     1981 caagaaaaat caagcagaaa attcccagat agttgacaaa gagagtgccc caaggaaagt
     2041 ctggactggc tggtaaccag gtggaaacta gatatcggac aagtccgccc aacatagcag
     2101 ctagaaaaat tcctagcgga taaaattgtt cttttttcat ttgatatttt gatcctgata
     2161 atcacgcgaa cgtttgagta tgtccgaaaa agtcgctaca atagtctcct gatagcgctt
     2221 gtcctctaca cgactcctaa ccttttcaaa aataacttct tctctcttag tatctaaaat
     2281 cggtttacct gaagctttct tataagcaac caccccctca actaaatgca ttcgttcttc
     2341 taacagtttg acaatttggt catcgatttg atcgatttct tgacgaataa tatctaaatc
     2401 catacagtct cctcctttat ttgaattatt gtatcaaaaa gccatcaatt aggctagtaa
     2461 aatcccaact ctcgataaga aaaatgcact gattgattac atcagcgcac ttttatactt
     2521 atactacttt agcaaccaat tcttctgcga attgttccaa gcgttcaata tcttcttcct
     2581 cggcagaaag atcaacttta acacactctg aacctttttc tgctcctatt gacacaaaga
     2641 cacggtcaaa gtcatcaaca gccttacaga attcatcgta gaaggtatct cctgagccga
     2701 ccactccgta gattttgcca ttcaagttga gatctgctag gtcttcgtag aagtccatca
     2761 tctcatctgg caattcacca tcaccataag tataggtcgc aacgatagcg atgtctgctt
     2821 ccaagaagtc tgaagcgtca acagttgtac attcatcaac atcaacatcc aagcccaagt
     2881 cacgtaattt gtctgctaca atatctgcaa tttcttcggt attaccggtc atactggcaa
     2941 atacaatttt tgctaatgcc atatcgtcct cctcaattta tctttctcca ttatatcaca
     3001 tctttttcat tttaaaaata aaaattcttg tgctacaatg aaatgagaaa gaattgaggt
     3061 tatttatgga gatttgccaa caaattttag aaaaaattaa agaatacgac accattatca
     3121 ttcatcgtca tatgaaacca gaccctgatg ccttgggaag tcaggtggga ttgaaagcct
     3181 tgctggaaca tcatttccca gaaaaaacca tcaaagccgt cggttttgat gaaccaactc
     3241 ttacttggat ggctgagatg gatcttgttg aagatagagc ctaccaaggc gcacttgtca
     3301 tcgtctgtga tacagctaat actgctcgta tcgatgataa gcgctatagt caaggtgatt
     3361 ttctcattaa gattgaccac catccaaatg atgatgtata cggtgacctg tcttgggtcg
     3421 atactagttc aagtagcgct agcgagatga ttaccctatt tgcccaaaca acccaactag
     3481 ccttggcaga tcgcgatgct gagttgctct ttgcaggaat tgtcggtgat acaggtcgct
     3541 tcctctaccc ttctaccact gcacggactc ttcgcctggc tgcttatttg agagaacata
     3601 actttgactt tgcggctctc actcgcaaaa tggacactat gagctacaaa attgctaaac
     3661 tgcaaggcta catctacgac catctggaag tggatgaaaa tggtgctgct cgcgttatcc
     3721 tgagtcaaga agtcttgaaa caatacaatg taaccgatgc tgaaactgca gccattgtcg
     3781 gtgcacctgg acgcattgac agagtgagtc tctggggaat ttttgtcgaa caggctgatg
     3841 gccactaccg agttcgctta cgcagtaaaa tccatcctat caatgaaatc gccaaggagc
     3901 atgatggtgg aggccaccct ctagcaagtg gtgctaattc ctatagccta gaagaaaacg
     3961 aaatcatcta ccaaaagtta aaaaacttgc ttaaaaactg ataaaatact tgccaaactt
     4021 ttcagaatct gatagactag tatggtaaca atctatggct cgcaaagaga ccatggcaga
     4081 aaggaaatat tgcaaaatga aaaaagatat ccatccagaa tatcgcccag ttgtcttcat
     4141 ggacacaact actggttacc aattccttag cggttcaaca aaacgctcta acgaaacagt
     4201 tgagttcgaa ggcgaaactt acccattgat ccgtgtggaa atttcatcag actcacaccc
     4261 attctacact ggacgtcaaa agttcactca agcagatgga cgcgtggatc gtttcaacaa
     4321 aaaatacggt ctcaaataat gataagagaa cagtttcggc tgttcttttt tgtttcttga
     4381 aatcaactgc tgttttcatg ttccagactc atctgtaggt ttggtttcca tgctactagg
     4441 caggaaggaa atagctgttt caacacgtcc ataatgagct gtactattgt cacgaaccac
     4501 actttcattg atggtccaag tggaattcat tttcttaaaa gcttctcgga ctttttccaa
     4561 atctttggag gcaatggcct gctctaaggt ttcaaaacga ggacttatac tcatctgctt
     4621 tcacaaagca ttctagtcca tctccgatta acgatgggct ttatcacctc cttctccagt
     4681 ccttgtatga tatcttaaag ttgattcatg acatcttcca aagtttgaaa ggctttattt
     4741 ttaaatccac gtttacgaat ctctttccac acttgttcaa tggggttcat ctctggtgtg
     4801 tacggaggaa taaatgcaaa gccaatatta gtcggaatct ttaaggtact tgatttatgc
     4861 catatagcat tgtccataac gagtaaaaga taatcatctg gataagcttg tgaaagctct
     4921 tctaaaaagg cgttcatcca ctcagtatta catccaccag ctcttaagaa aaatgattcg
     4981 cctgtatggg catcaacagc tccataacaa tagcgaaatt ctcgtatata gtgactatgg
     5041 acatgtggac ctactcctat tggagaccaa caagatccca gtttactgat tctaccgaaa
     5101 ccagcctcat cttggtacat cgagcgaacc ttatgaaaag gtctactggt tttaaagcgc
     5161 tttcttttct tcttgaattg agattttatt tttagacgcg acaatggttt gagcgtctgc
     5221 tttcttagga tgttctggac gtggcataat atttcgccaa ccatggcgct tcaacagttg
     5281 atagaaggca tcacgtgtgt aggaacgac
//
LOCUS       DAVKJU010000089         4934 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVKJU010000089 DAVKJU010000000
VERSION     DAVKJU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.089"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            108..704
                     /locus_tag="VW191_001905"
                     /pseudo
     CDS             108..704
                     /locus_tag="VW191_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            780..1229
                     /locus_tag="VW191_001906"
                     /pseudo
     CDS             780..1229
                     /locus_tag="VW191_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            1242..2333
                     /locus_tag="VW191_001907"
     CDS             1242..2333
                     /locus_tag="VW191_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624621.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQTIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRMLALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNR
                     IRLRINTQKLNIFSADGSQNLIKGVNHGT"
     gene            2323..3968
                     /locus_tag="VW191_001908"
                     /pseudo
     CDS             2323..3968
                     /locus_tag="VW191_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4071..4763)
                     /locus_tag="VW191_001909"
     CDS             complement(4071..4763)
                     /locus_tag="VW191_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU9624622.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1554 a         1048 c          846 g         1486 t
ORIGIN      
        1 aaagtgtact gttatagatt caatatacta tagaatagaa ttgtaggttt taaaaacgct
       61 tacattgttt taaaataaac ttaaataaca caacggaggt atccatcatg aaaaagaaat
      121 ggatgtatta tgctgcttgt tcttctaatg aatctgccga tgacagttca tctgataaag
      181 gagacggcgg ttcgctagtc gtttattgac caaactcaga gggcttaatt ggagcaacta
      241 ttcctgcctt tgaagaaaaa tatggtatca aaatagaact gattcaagct ggtactggag
      301 aacttttcaa aaaactagag tcagaaaaag aagttcctgt agctgatatt atctttggtg
      361 gttcttatac acaatatgct acccacggag aactctttga aaactatatt tcaaaagaaa
      421 atgataatgt tatcaaagaa tatcaaaaca caactggcta ctctactcct tatacactag
      481 atggtagtgt tttaatcgtc aaccctgatt taactaaagg catgaacatc gaaggatata
      541 acgatcttct caaacctgaa ctaaaaggaa aaatcgcaac tgctgaccca gcaaactctt
      601 ctagcgcctt tgctcaatta acaaatatgc tacaagctca aggtggttac aaagatgata
      661 aggcttggtc ttatgtaaaa gatcttttca cacttattga tggcatagtg aaatgaaata
      721 agaacaggac aaattaatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga
      781 tgtgtactat tctagtttca atatactata aaatcggttc aagttcatct ggtgtctata
      841 aagcagtcgc tgatggagaa atgactgttg gtctctctta tgaagatcca gcagttaaac
      901 tcttaaatga cggagctaac attaaggtag tctatccaaa agaaggaacc gtcttcctac
      961 ctgctagtgc tgctatcgtt aaaaaatcta aaaatatgga aaatgccaag aaatttatcg
     1021 attttattat ctctcaagaa gtacaagata cacttggtac aaccactact aaccgtcctg
     1081 ttcgtaaaaa tgctaaaaca agcgaaaaca tgaaaccaat tgacaaaatc aaaacactca
     1141 cttaagatta tgattatgtc atcaagaata aatcagatat cgttaagaaa tacaacgaag
     1201 tctttacaga tatccaatct aaacagtaaa agaggttcac tatgagtgag atcgaaatta
     1261 ttaacgccaa aaaaatctac cacgatgtcc ctgttattga gaatttgaac attacaattc
     1321 caaaaggaag tctctttacc cttcttggag cttcaggatg tgggaaaacg acccttcttc
     1381 gtatgattgc aggtttcaac agtatcaaag atggagaatt ttacttcgat gatacaaaaa
     1441 tcaataatat ggaacccagc aaacgcaata tcgggctggt tttccaaaac tacgctattt
     1501 tcccacattt gactgtccga gacaacgttg cttttggtct tatgcaaaag aaggttccaa
     1561 aagaagaatt gattcaacag accaacaagt atcttgaact catgcaaatt gctcaatatg
     1621 cggatcgaaa gcccgataaa ctcagtggtg gacaacaaca acgtgtcacc ttggcatgcg
     1681 ccttagcggt taatccaagt gttctcctca tggacgagcc acttagtaat ctggaggcca
     1741 aacttcgctt ggatatgcgt caaaccatcc gagaaatcca acacgaagtg ggaattacaa
     1801 ctgtttatgt aacccacgac caagaagaag ccatggctat ttcagaccaa attgctgtta
     1861 tgaaagatgg ggtgatccaa caaatcggcc gaccaaaaga actctatcat aaaccagcta
     1921 atgagtttgt ggcaaccttt atcggacgca caaatattat ccctgccaat cttgaaaaac
     1981 ggagcgacgg cgcttatatc gtcttttcag atggctatgc ccttcgaatg ctagctcttg
     2041 atcaggttga ggagcaagct attcatgtaa gcattcgtcc cgaagagttt atcaaagatg
     2101 aatctggaga tatcgaagga actattagcg atagcgtcta tcttggacta aatacggagt
     2161 atttcattga gacaggtttt gcctcaaaaa ttcaagttag tgaagaatca acttttgaag
     2221 aagatctaca aaaagacaat cgtattcgtc tacgaatcaa tacgcaaaaa ttaaacatct
     2281 tttctgcaga tggttcccaa aacctgataa aaggagtcaa ccatggaacg taaaaaacta
     2341 aatatttgga cagcctcctc tttcttcatc tttcttacct atcttgtctt tctcgtttat
     2401 cctatcgtta ccgtgctcaa gtaagcactt atacatgaag gataattctc actagctaat
     2461 tttgtcactt tctttagtaa aacctactac tctgagacac tagtcaacag tttcagggtt
     2521 tccattaccg ctactgtcac ttccttagtt gtaggaaccc tattagctta tctcttctct
     2581 atgtatgact tcaaggggaa gaaatttcta caaatattga ttatcattgc ttccatgtca
     2641 gctcctttcg taggagccta ctcctggatt ctcttgctag gacgaaatgg ggtcatcact
     2701 aaatttttga caaatgccct tcatcttcca gctatcgata tttatggatt caaaggaatt
     2761 atacttgtct ttacactgca actattccca ctggtatttc tatatgttgc tggaacaatg
     2821 aacagtattg acaattctct acttgaagca gctgaaagca tggggtcctt cggatttaaa
     2881 cctatcgtaa cggttgtttt acctctccta gttccaacct tactagcagc tccttgcttg
     2941 tatttatgag agcattctca gactttggaa cgcctatgtt gattggcgaa ggatatcgga
     3001 ctttccctgt cctgatttat acccaattta ttagcgaggt tggaggaaat tctgcttttg
     3061 caattatggc gattatcatt gccttggcaa ttttccttat ccaaaaacac attgcaaacc
     3121 gctacagttt cagcatgaat ccgctccatc caattgagcc taaaaaaact acaaaaggaa
     3181 aaatggctgc catttatgca acagtctacg gaattatctt tatctatatt ttacctcaaa
     3241 tctacttaat ttatacctct ttcctaaaaa catcaggtat ggtatttgtt aaaggttatt
     3301 ctccaaacag ttacaagcta gctttcaatc gtatgggatc tgctattttc aataccattc
     3361 gtatcccttt gattgcctta gttctagttg ttctatttgc gacatttatc tcctacctag
     3421 ccgttagaaa acggaatttg tttacaaact taattgacag cctcagtatg gtaccttata
     3481 ttgtaccagg aaccgttcta gggattgcct tcatttcttc cttcaatact ggtctatttg
     3541 gaagtggatt tcttatgatt acagggactg ctttcatctt gattatgtct ctatctgtca
     3601 gaagattacc ttatactatt cgctcatctg ttgctagctt acaacaaata gcaccaagta
     3661 ttgaagaagc tgctgaaagc ttaggaagta gtcgtctcaa tacctttgct aagattacaa
     3721 ctccaatgat gctatctggt atcatttctg gagccatctt atcttgggtc acaatgattt
     3781 caaaactctc tacttctatc ctcctctaca atgtcaaaac aagaacaatg actgtagcta
     3841 tttatacaga ggttctcaga ggaaattacg gtgtagccgc agccttgtca actatcctga
     3901 ctgttctaac agtaggttcc ttgctcttgt ttatgaaaat ctctaaaagc aatagcatta
     3961 cactttagtt ttccacatca aaaacagccg aaaggagtac cctcggctgt tttttcttat
     4021 cttgatattc ttatcaaatc cctagctcat ggcaagcaag tctagactga ttaaatagag
     4081 ccttcttcca tcttctcacg tcctagctct cggtaactac ggtcaccgac aacttcaacc
     4141 ctaaactgac cgtcctcata ttcaagaatc gtcacgctac cattatctag accatgcgga
     4201 tgcatgccat taatcagata aacaatggtt ccaatagtca ttccatggct gacaacaagg
     4261 gcgttacctc caccttgatc ttccatttct tttgcaatca tttcaaagcc ttccttgatt
     4321 cggccactga gtttttccca gccttcagcc caaccagctg tatcgacctc taccaagccc
     4381 tcagccagtt cagcataaga caattggtga acgtggtcca cattaaagat acgaggaata
     4441 atgcccatga aaagatcgcc atcataggct ccatcaaaac taccgaaaca ccattctctg
     4501 atacgcttgt ccatgcgata agggattttc ccctgcaagc caagttcttc aaggataatt
     4561 cccatggtct gaatggtacg accagaatca ctcgaataag cgcgctcaaa ctgtagatca
     4621 gattctcgca aaccgattcc taactcttga atccctcgtt caccttcagc agttaaggga
     4681 gtatcgctcc aaccttgcgc gcgaccaatc gtgttaaaca tggtcttgcc atgacgtacc
     4741 aaatacaatc gtacttttac cattttccgt cctccgtttg cttctattat atcatgaatg
     4801 ataaaataaa agccacccat acaggcgact tttgaaggag attattatga aaaagtttag
     4861 gagttcatta aataaagata ttagatgaaa atcaaattca aactaattca gtgttatctg
     4921 tttcaaataa tatt
//
LOCUS       DAVKJU010000090         4283 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVKJU010000090 DAVKJU010000000
VERSION     DAVKJU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.090"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..991
                     /locus_tag="VW191_001910"
     CDS             <1..991
                     /locus_tag="VW191_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEU9624623.1"
                     /translation="MCIVFSACSSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPE
                     RVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLD
                     GLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKA
                     LGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDP
                     RANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKD
                     PLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
     gene            981..1988
                     /locus_tag="VW191_001911"
     CDS             981..1988
                     /locus_tag="VW191_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9624624.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            1988..2995
                     /locus_tag="VW191_001912"
     CDS             1988..2995
                     /locus_tag="VW191_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU9624625.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFVLLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            2995..3789
                     /locus_tag="VW191_001913"
     CDS             2995..3789
                     /locus_tag="VW191_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624626.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
BASE COUNT         1553 a          627 c          732 g         1371 t
ORIGIN      
        1 tatgtgtata gtttttagtg cttgttcttc taattctgtt aaaaatgaag aaaatacttc
       61 taaagagcat gcgcctgata aaatagtttt agatcatgct ttcggtcaaa ctatattaga
      121 taaaaaacct gaaagagttg caactattgc ttggggaaat catgatgtag cattagcttt
      181 aggaatagtt cctgttggat tttcaaaagc aaattacggt gtaagtgctg ataaaggagt
      241 tttaccatgg acagaagaaa aaatcaaaga actaaatggt aaagctaacc tatttgacga
      301 tttggatgga cttaactttg aagcaatatc aaattctaaa ccagatgtta tcttagcagg
      361 ttattctggt ataactaaag aagattatga cactctatca aaaattgctc ctgtagcagc
      421 atacaaatct aaaccttggc aaactttatg gagagatatg attaaaattg attcaaaagc
      481 cttaggtatg gaaaaagaag gtgatgagtt aatcaaaaat actgaagctc gtatatccaa
      541 agaattagaa aaacatccag aaatcaaagg aaaaatcaaa ggaaaaaaag tattatttac
      601 tatgattaat gctgcagata catcaaaatt ctggatttat actagcaaag atccaagagc
      661 aaattattta acagatttag gtctagtttt ccctgaatca ttaaaagaat ttgagagtga
      721 agatagtttt gcaaaggaaa tttctgcaga agaagcaaat aagataaatg atgctgatgt
      781 aatcataact tatggtgatg ataaaactct tgaagcttta caaaaagatc ctcttttagg
      841 taaaataaat gcaattaaaa atggtgccgt tgctgtaatt ccagataata caccgttagc
      901 agcctcatgc actccaacac cactttcaat aaactatact attgaagaat atctaaatct
      961 tttaggaaat gcatgcaaaa atgcgaaata aaaaacaaat aaacctaggc ataatttttg
     1021 taatctgcct aggtcttctt attacaatat ttttgtcatt aaagcttgga acaaaagaaa
     1081 ttaatatcag agatttttta gcagcttttg gaatgggtaa tacaaatgat gattttatta
     1141 aatcaattat atataaaaga atacctagaa ctatttttgc aattttagca ggttctagtc
     1201 ttgccataag cggtgtattg atgcaatcag ttactagaaa cccaatagct gatccaggta
     1261 tactcggtat aaacacagga gcaagtctta gtgtagtaat tggtctttct tttttaggaa
     1321 tttcatcaag cataagccat ataagttttg caatcattgg tggcttagta agtgcaattt
     1381 ttgtatacgc gattgctgta agcggaaaag caggccttac ccctataaaa cttgccttat
     1441 caggaacttg tgttagtatg gctttaagca gttttgtaag ttttttaatt ttaccgaata
     1501 ataacgtctt agacaaattt agattttggc aaataggtag ccttggagca gctacattat
     1561 cttctatatc tacactacta ccttttataa ttttaggtca cttgatagct atatttattt
     1621 catcagattt aaacgcttta gctatgggtg atgaaatggc tgttggtctt ggagttaatg
     1681 ttaataggat aagatcactt gcaataattg caagtgtgct tttatgttca agtattactg
     1741 caattggtgg acctattggc ttcgtaggtc ttatagttcc tcacttttgt ggcttattta
     1801 taagcaaaga tatacgcaca atgaccattt cttcatcttt tataggtgca gagctcttgc
     1861 ttatatgtga tataatcggc cgtatgttag gtaaaccagg tgaaattgaa gtagggataa
     1921 ttactgcaat aatcgggggt ccagtactta tttatgtaac tatgaaaaat agaggggtta
     1981 ataactaatg caaaatttaa ttataggtat tcaaaaaaga aaaaatagaa taacactatt
     2041 ttcctcacta tttttattaa taataatcag tctatcattt ttcgttttac ttatcggaga
     2101 tgaaagttat tctttttcaa ctttgattaa agtcttaaat agtgaaactg ttcctggagc
     2161 tagtttttcg attatggaaa ttagattacc aaaattatta gcaggaatta tagctggctg
     2221 gtcttttgga ttggcaggat ttatctttca aactatgtta agaaatcctc ttgcaagtcc
     2281 tgatataatc ggtgtcacaa gttcttcatc tattgcagcg gtcttttgca tattggtatt
     2341 aaaaacaaat agtttaacta ctggaattat ttcaataact tgtggactaa catcatcttt
     2401 aatattattt ttactagcta aaaaagatgg tttttcagca gcaagactga taatattagg
     2461 cattggtttt caagctgtca caagagcagg cacctcattt ttattgttga aagtagcaag
     2521 atatgaatta caagaagtta tgagatggct cagtggctct ttatctttta caaagttaga
     2581 tgacatacct cttgttctaa tagtaagtat tattgctact atattagttt tattttttaa
     2641 taaaagacta gaaattattg aacttggtga agaaatagca atcggacttg gagcaaatcc
     2701 cgagctttca aggcttgttt taattttttg cgctgtatct ttaactgctt tttctacttc
     2761 aattacagga ccaatagctt gtatatcttt tttagctggt cccatagcct taaatattgg
     2821 caagaaaaga agcccaatat tagctggatt ggttggaatt ttactagttt tgttatcaga
     2881 catattctct caaaatattt taccagctag atatccagta ggtgttgtaa ctggcttgtt
     2941 aggttcacca tacttaatat atttactaat aaaaatgaac aggaggaata tataatgaaa
     3001 ggtctgtggt caaataatct tacctgcggt tatgatgaaa aaataatctt ggaaaatata
     3061 aatataaaaa tacctgaaga aaaaatatca gttattattg ggtcaaatgg ttgtgggaaa
     3121 tcaacactca ttaaaacctt gtctcgactt ataaagccat tagagggaga agtattgctt
     3181 gataataaat caattaattc ttataaagaa aaagatttag caaaacacat agctatatta
     3241 cctcaatctc caataatccc tgaatcaata acagtagctg atcttgtaag ccgtggtcgt
     3301 ttcccctaca gaaagccttt taagagtctt ggaaaagatg accttgaaat aataaacaga
     3361 tcaatggtta aggccaatgt tgaagatcta gcaaataacc tagttgaaga actttctggg
     3421 ggtcaaaggc aaagagtatg gatagctcta gccctagccc aagatacaag tatcctactt
     3481 ttagatgagc caactactta cttggatatc tcatatcaaa tagaactatt agacctcttg
     3541 actgatctaa accaaaaata taagacaacc atttgcatga ttttgcacga tataaatcta
     3601 acagcaagat acgctgatta cctatttgca attaaagaag gtaaacttgt tgcagaggga
     3661 aagcctgaag atatactaaa tgataaacta gttaaagata tctttaatct tgaagcaaaa
     3721 attatacgtg accctatttc caattcgcct ctaatgattc ctattggcaa gcaccatgtt
     3781 agctcttaat aattgaatat tggaattcat ttaaaagtcg caaattttga actgaccctc
     3841 aaaagttagg ccaaaaaact aactaaagga ggtcagtttt ttaatggcaa atacagcaca
     3901 gaatttaaga taaaattagt aaaagaatat catgaatgaa aaataggata tagagaataa
     3961 cagcaatatt aaaaagatca ggactgataa taaacaaaaa gaaagtacaa cgactagttc
     4021 aaaagctaaa acttcaagta aaaagttatt caagaaaatc tagaaaatac tcatcctaca
     4081 aaggacaggt aggaaaaata tcagacaaca aaataaaaag aaatatttat taccgaaaaa
     4141 aaggggaaat cgagtcaagt accaacaggc tcatctattc caaaaagata tttactcgat
     4201 ggaaaaactc ctagaattag tgctacaaat attgacaatg gtatcttagg ttactacgaa
     4261 gatatagatg ataaaaatta tag
//
LOCUS       DAVKJU010000091         4217 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVKJU010000091 DAVKJU010000000
VERSION     DAVKJU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.091"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            99..1610
                     /locus_tag="VW191_001914"
     CDS             99..1610
                     /locus_tag="VW191_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEU9624627.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1632..2963
                     /locus_tag="VW191_001915"
     CDS             1632..2963
                     /locus_tag="VW191_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU9624628.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(3369..3659)
                     /locus_tag="VW191_001916"
                     /pseudo
     CDS             complement(3369..3659)
                     /locus_tag="VW191_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3647..3898)
                     /locus_tag="VW191_001917"
     CDS             complement(3647..3898)
                     /locus_tag="VW191_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEU9624629.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
BASE COUNT         1187 a          857 c          826 g         1347 t
ORIGIN      
        1 atggtaaaac aaatataaat agaaagcgtt ttcttgaatg tttatttaag aaagtagttg
       61 gttttttaca ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa gctaaaaaag
      121 ggtttaagat gccttcatct tacaccgtat tattgataat cattgctatt atggcagtgc
      181 taacttggtt tatccctgcg ggggccttta tagaaggtat ttacgagact cagcctcaaa
      241 atccacaagg gatttgggat gtcctcatgg caccgattcg ggctatgcta ggtactcatc
      301 cagaggaagg ttcgctcatt aaagaaacga gcgcagcgat tgatgtagcc ttcttcatcc
      361 ttatggttgg gggtttcctt ggcattgtca acaaaactgg tgctcttgac gtagggattg
      421 cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt aattttggta ctgatgcctt
      481 tgtttgccct cggtggtaca acttatggta tgggggaaga aacaatggcc ttctatccac
      541 tccttgtgcc agttatgatg gccgttggtt ttgatagcct gactggtgtt gcaattattt
      601 tgctcggttc tcaaatcggc tgtttggcat ctactctgaa tccatttgcg acaggtattg
      661 cttcagcgac tgcgggagtt ggtacagggg acggtatcgt acttcgtctg atcttctggg
      721 ttaccttgac tgctcttagt acttggtttg tttaccgtta tgcggataag attcaaaaag
      781 atccgactaa gtcactggtt tatagtactc gcaaagaaga tttgaaacac tttaacgtag
      841 aagaatcttc atctgtagaa tctacactta gcagcaaaca aaaatcagtt ctcttcttat
      901 ttgtgttgac attcatcttg atggtattga gcttcattcc atggacagac cttggcgtta
      961 ccatttttga tgactttaat gcttggttga ctggtcttcc agttattggt aatattgtcg
     1021 gttcatctac ttctgcacta ggtacttggt acttcccaga aggcgcaatg ctctttgcct
     1081 ttatgggtat cctgattggt gttatttatg gtcttaaaga agataagatt atctcttcct
     1141 tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt gctcgtggta
     1201 ttcaagttat catgaacgac ggtatgatta ccgatacaat cctcaactgg ggtaaagaag
     1261 gcttgagcgg tctatcttca caagtcttta tcgttgtaac ttatatcttc tatctaccta
     1321 tgtcattctt gatcccatct tcatctggtc ttgccagcgc aactatgggt atcatggctc
     1381 cacttggaga atttgtaaat gtccgtccta gcttgattat cactgcttac caatctgctt
     1441 caggtgtctt gaacttgatt gcaccaacat ctggtattgt gatgggagct cttgcacttg
     1501 gacgtatcaa cattggtact tggtggaaat tcatgggcaa actcgtagtc gctattattg
     1561 tagtgaccat cgcccttctt ctccttggaa ccttccttcc attcctataa aatagtgagt
     1621 gaggtgattc catgaaaata gatataacaa atcaagttaa agatgaattt cttatatcat
     1681 taaaaacctt gatttcctat ccttcagtac tcaatgaagg agaaaatgga acaccttttg
     1741 gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat ttgtcgagac ataggtttca
     1801 ctacctatct tgaccctaaa ggttattacg gatatgcaga aatcggtcag ggagcagagc
     1861 ttctggccat tctctgtcat ttggatgttg ttccatcagg tgatgaagca gattggcaga
     1921 caccgccatt tgaagcaact atcaaagacg gctgggtatt cggacgtggt gtccaagatg
     1981 ataaaggtcc ttcgcttgca gctctctatg cagtaaaaag cttgctggac caaggtattc
     2041 agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga ggaaaccctc tggcgctgca
     2101 tggcacgcta caataccatc gaagaacagg ccagcatggg ctttgcacct gactcatctt
     2161 ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa acttcatggc cctggatcgg
     2221 atcaactaga gcttgaagta ggaggcgcct ttaacgttgt accagacaag gccaactacc
     2281 aaggtcccct ctatgaacag gtttgtaacg atctcaaaga agctggttat gattaccaat
     2341 ccactgaaca aaccgtaacg gttctcggag tgccaaagca tgctaaggat gctagtcaag
     2401 gtatcaatgc tgtcatccga ctagctacca ttcttgctcc tctccaagaa caccctgctc
     2461 tcagctttct tgcaacacaa gcaggtcaag acggcacagg aagacaaatc tttggtgata
     2521 tagcagatga accttctggt cacctatcct ttaatgtcgc aggtctcatg atcaatcatg
     2581 aacgttctga aatccgtatt gacattcgga ctcctgtctt agctgacaag gaagaactag
     2641 taaagttgct tacaagatgt gcacaaaact accaactccg ctacgaagag tttgactatc
     2701 tagcgcctct atacgtcgca aaagacagta aactcgttag cacactgatg caaatctacc
     2761 aagaaaagac tggcgataac agtcctgcta tttcatccgg tggtgccact tttgctcgca
     2821 ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag acagaacatc
     2881 aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt tatgccgaag
     2941 ccgtctatcg acttgcaact taatcaggca actgtttcta caaaaaaaaa tcgaccgatt
     3001 aatgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtg ttttattatg
     3061 aaattgagtt atgaagataa agttcagatc tatgaactaa gaaagcaagg acaaagcttc
     3121 aaacagcttt caaaaagatt tggtgtggat gtttctggtc taaagtcatc tgaatctttg
     3181 agatgagctt tataaatcgc ttttttcagt ttttgcactg gtatttcgat aaactcaaac
     3241 tttttagccg ttgtattgcc tgattttata gtatgttgaa actagaatag tacacctcta
     3301 cttctaaaac atttttagaa atcaatttga ctgtcctgat tgatttgtcc tgttcttatt
     3361 tcattttact atatttgagc cacttcgtct ttaacggctt tattcataag ctcttgtaat
     3421 ttttctttac tatcaattac ttctgatttt ccgttgtaat ttattgtaat aggttttaac
     3481 ttacctaatt tctcgacacg ctcattaatt tgatcttttt tgaaggctgc atatgttttt
     3541 cctaatattt tttcgaaaat atatttatca gatagcggtt tgtcttcttc ttcagcttgg
     3601 tttttgtatt aatttgaaac ataaggaaca aatccttcat agtaacctaa tgctcccata
     3661 agttcaaaag cttgttttct aattcaagcc attgcaactc agatttcagc ttttcagata
     3721 aatcctgctc atccaaataa tgacttaaaa ttagtgctga actcgtttct gtatcctgta
     3781 caggctgatc acccatacca tcaaaaaata aactcgttcc tagcaagacc gaacaagctc
     3841 ctattgcata tggcctcaaa gaaaaacgct gctttctctc aaattgaaat tctttcatcc
     3901 catctcccat cattcattat tactgtatat tttgtatatc agaaatagtt tgtattcaca
     3961 aatctttcta gttattccct tatcattcct aattaagaga gataacatac aataattttt
     4021 agttaaatgt atatcgatgt tttttgtttt tcttaataaa cgcaatacaa aaagagcctg
     4081 ttgccaagct ctttgtactc aatgaaaatc aaagagcaaa ctaggaaact agccacaggt
     4141 tgctcaaaac accgttttga ggtagtagat aagactgacg aagtcagctc aaaacaccgt
     4201 tttaaggtag tagataa
//
LOCUS       DAVKJU010000092         4178 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVKJU010000092 DAVKJU010000000
VERSION     DAVKJU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.092"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            713..1705
                     /locus_tag="VW191_001918"
     CDS             713..1705
                     /locus_tag="VW191_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9624630.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1707..2525
                     /locus_tag="VW191_001919"
     CDS             1707..2525
                     /locus_tag="VW191_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEU9624631.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2527..3312
                     /locus_tag="VW191_001920"
     CDS             2527..3312
                     /locus_tag="VW191_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU9624632.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3410..3628
                     /locus_tag="VW191_001921"
     CDS             3410..3628
                     /locus_tag="VW191_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEU9624633.1"
                     /translation="MKDLPEWFGIPESTQAYIEGTTTLQVWTAYQESDLTGFVRLSYS
                     SEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3613..3810
                     /locus_tag="VW191_001922"
     CDS             3613..3810
                     /locus_tag="VW191_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU9624634.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEASNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
BASE COUNT         1163 a          718 c          905 g         1392 t
ORIGIN      
        1 tcatcagaat gataaaatta atcaattgat atctgattac aaacagaata tgaaagcttt
       61 ttatatcact attgaaaaat ttatacgaaa tgatgaaagc cttaagatga tgaaagcctt
      121 aagtgttatt ttataaaggt tatttcaagt cgttccaagg taacaagtct agatcagatt
      181 gaagctgata aaacgataca aagaaaatat tcaagtgagc taaaaaaatt tattggattt
      241 tataatgaga ttatttgtga ggaaaatagt ttcctacatg tacgaaagag gtgatcgagt
      301 tggtttaggt agtcgatgcg tgagttgata attctcaggg tatggacttc tttttcatga
      361 atgaggtaaa agagcaggta ttgtttagag acaatcattc tgagcatatt ttctggatag
      421 agggagtatc cgattttatg atcaaagtta ataccgccct ctggtgagaa gatgagtagg
      481 ttggtaattt aaactattaa acagaatttt tgattaaaag tattatttta tgagagaaat
      541 cctaatttca caatccatag gcaaactctt gcatttcgtt ttttattgga ctataatagg
      601 ttggtataaa gccttttgta gtaataaaat gtagaaggtg tagaaagcaa ggatttagaa
      661 tatttgtagt taaaaacaca atgttgctat tccttacgat agggagatag atatggcaat
      721 gatagaagtg gaacatcttc agaaaaattt tgtgaagact gttaaggaac cgggcttgaa
      781 gggggctttg cgctccttta ttcatcctga aaagcagacc tttgaagcgg tcaaggattt
      841 gacctttgag gttccaaaag ggcagatttt aggatttatc ggggcaaatg gtgctgggaa
      901 gtcgacaacc attaaaatgc tgacaggaat tttgaaacca acatctggtt tttgtcggat
      961 taacggcaag attccccagg acaatcggca agattatgtc aaagatattg gcgtagtctt
     1021 tggacaacgc acccagctat ggtgggattt ggctctgcaa gagacctaca ctgtcttaaa
     1081 agagatttat gatgtgccaa actcgctctt tcataagcgc atggactttt tgaatgaagt
     1141 cttggatttg aaggacttta tcaaggatcc cgtgcggact ctttcactgg gacaacggat
     1201 gcgggcggat attgcggcct ccttgctcca caatcccaag gttctttttt tagatgagcc
     1261 gaccattggt ttggacgttt cggttaagga taatattcgt cgggcaatta ctcagatcaa
     1321 tcaagaggaa gaaactacca ttcttttgac cactcacgat ttgagtgata ttgagcaact
     1381 ttgtgatcgg attttcatga ttgacaaggg gcaagagatt tttgatggaa cggtgagcca
     1441 actcaaggag acctttggta agatgaagac tctctctttt gaactgctac caggtcaaag
     1501 tcatctcgtc tctcactatg aaggtctgtc tgatatgacc attgatagac aaggaaacag
     1561 cctcaacatt gaatttgata gttctcgcta ccagtcagct gacattatca agcaaaccct
     1621 gtctgatttt gaaatccgcg atttgaagat ggtggatacg gatattgagg atattatccg
     1681 tcgcttctac cgaaaggagc tctaggatga tcaaattgtg gagacgttat aaacccttta
     1741 tcaatgcagg ggttcaggag ttgattactt accgagtcaa ctttattctc tatcggattg
     1801 gcgatgtcat gggggctttt gtggcctttt atctctggaa ggctgtcttt gattcttcgc
     1861 aagagtcttt gattcagggc ttcagtatgg cggatatcac cctctacatc atcatgagtt
     1921 ttgtgaccaa tcttctgact agatccgatt cgtcctttat gattggggag gaggtcaagg
     1981 atggctccat tatcatgcgt ttgttgcgac cagtgcattt tgcggcctcc tatcttttca
     2041 ccgagcttgg ttccaagtgg ttgattttta tcagcgttgg ccttccattt ttaagtgtca
     2101 ttgtcttgat gaaaatcata tcgggtcaag gtattgtaga ggtgctagga ttaactgtcc
     2161 tttatctttt tagcttaacg ctcgcctatc tgattaactt tttctttaat atttgctttg
     2221 gattttcagc ctttgtgttt aaaaatcttt ggggttccaa cctacttaag acttccatag
     2281 tggcttttat gtcggggagt ttgattccct tggcattctt tccaaaggtt gtttcagata
     2341 ttctttcctt tttgcctttt tcatccttga tttatactcc agttatgatc attgttggaa
     2401 aatacgatgc cagtcagatt cttcaggcac tccttttgca gttcttctgg ctcttagtga
     2461 tggtgggatt gtctcagttg atttggaaac gggtccagtc ctttatcacc attcaaggag
     2521 gttagtatga aaaaatatca acgaatgcat ctgattttta tcagacaata catcaaacaa
     2581 atcatggaat ataaggtgga ttttgtggtt ggtgtcttgg gagtctttct ggctcaaggc
     2641 ttgaatctct tgtttctcaa tgtcatcttt caacatattc cattcctaga aggctggacc
     2701 tttcaagaga tagctttcat ttatggattt tccttgattc ccaagggaat ggaccatctc
     2761 ttttttgaca atctctgggc actagggcaa cgcctagtcc gaaaagggga gtttgacaag
     2821 tatttgactc gtcccatcaa tcctctcttt cacatcctag ttgaaacctt tcagattgat
     2881 gccttgggtg aactcttagt cggtggtatt ttattgggaa caacagtgac cagcattgtt
     2941 tggactcttc caaaattcct gcttttccta gtttgtattc cttttgcgac cttgatttat
     3001 acttctctta aaatcgcaac agccagtatc gccttttgga ctaagcagtc aggcgccatg
     3061 atttacatct tctatatgtt caatgacttt gctaagtatc cgatttctat ttacaattct
     3121 cttcttcgtt ggttgattag ctttatcgtg cctttcgcct ttacagccta ctatccagct
     3181 agctatttct tacaggaaaa ggatgtgttc tttaacgtag gaggtttgat gttgatttct
     3241 ctggttttct ttgttatttc ccttaaactt tgggataagg gcttagattc ctacgaaagt
     3301 gcgggttcgt aaaagctaaa gtaagactaa aatcaagaaa ggaacttatg atgtttgtaa
     3361 ttgaagaagt caaggatgaa aatcaaaaaa ggcagttgtc gctgaggttt tgaaggattt
     3421 gccagaatgg tttggaatcc cagaaagcac acaagcctat atagaaggaa ccacgacact
     3481 gcaagtttgg accgcctatc aggagagtga tttgactgga tttgtaaggt tatcctattc
     3541 gagtgaagat tgtgcagaga ttgattgtct cggcgtaaaa aagcttatca aggtagaaaa
     3601 attgggagcc aattgcttgc tactttagag agtgaagctc gtaaaaaagt tggttatctg
     3661 caggtcaaaa cagtggcaga agcttctaat aaagattatg atcgaacaaa tgacttttat
     3721 cgaggtcttg gctttaaaaa gttagagatt ttttctcagc tatggaatcc gcaaaatcct
     3781 tgtcagattt tgattaaaaa gcttgaataa tattacttga catctattct cagagtgcca
     3841 tactgtaagt gtaatcgccg atttagctca gttggtagag caaggcactc gtaaagccta
     3901 ggttataggt agataaacga ctgaggagtt gaaaaaatag ataggtagaa gataaccgtt
     3961 aagccttatt cttagcggtt atttatatta tttaatagcg ctgatatttc atcaattatg
     4021 cctgttttcg tgtttctggt agttgttcaa gtttattgct actatttttg atggtatgaa
     4081 tgtgcttata atgtatcccg gttaacgaaa gttttggact tatactcttc gaaaatctct
     4141 tcaaaccacg tcaacgtcgc cttgccgtgc gtatggtt
//
LOCUS       DAVKJU010000093         4078 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVKJU010000093 DAVKJU010000000
VERSION     DAVKJU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.093"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            247..1533
                     /locus_tag="VW191_001923"
     CDS             247..1533
                     /locus_tag="VW191_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEU9624635.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFATKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDVY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
     gene            1548..1859
                     /locus_tag="VW191_001924"
     CDS             1548..1859
                     /locus_tag="VW191_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624636.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHNS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            2119..2847
                     /locus_tag="VW191_001925"
                     /pseudo
     CDS             2119..2847
                     /locus_tag="VW191_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            3283..4074
                     /locus_tag="VW191_001926"
     CDS             3283..4074
                     /locus_tag="VW191_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624637.1"
                     /translation="MNFALNKGEIVGLAGRNGVGKSTLMKILVQNNQPTSGNIISSDN
                     VGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQEIFRSLIQELDLTQSINKKVKTYSL
                     GTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLSVLKNLALHENVGILISSHKL
                     EDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGDIVQEE
                     GLSITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
BASE COUNT         1330 a          637 c          847 g         1264 t
ORIGIN      
        1 gtagtcatct aaggactaat ttatatattt ttacgagtca gtatcttggg atactggttt
       61 ttactttttt agactttttg actacttgtt aaaactgggg taattttcga ctgtttaata
      121 gttattatgc aaagtctaaa agattagaat tgtcaaaaca atccgtctag gcttgatttt
      181 atcctttatt tactataaaa tgagaaggaa aaatgtcaaa cttttatatt gcaaatagga
      241 gaaatcatga caaaaacatt aaaacgtcct gaggttttat cacctgcagg gactttagag
      301 aagctaaagg tagctgttca gtatggagca gatgctgtct ttatcggtgg tcaggcctat
      361 ggtcttcgta gccgtgcggg aaactttact ttcgaacaga tggaagaagg cgtgcagttt
      421 gcgaccaagt atggtgccaa ggtctatgta gcggctaata tggttatgca cgaaggaaat
      481 gaagctggtg ctggtgagtg gttccgtaaa ctgcgtgata tcgggattgc agcagttatc
      541 gtatctgacc cagccttgat tatgattgca gcgactgaag caccaggcct tgaaatccac
      601 ctttctaccc aagccagtgc cactaactat gaaacccttg agttctggaa agagctaggc
      661 ttgactcgtg tcgttttagc gcgtgaggtt tcaatggaag aattagctga gatccgcaaa
      721 cgtacagatg ttgaaattga agcctttgtc catggagcta tgtgtatttc atactctgga
      781 cgttgtactc tttcaaacca catgagtatg cgtgatgcca accgtggtgg atgttctcag
      841 tcatgccgtt ggaaatacga cctttacgat atgccatttg ggaaagaacg taagagtttg
      901 cagggtgaga ttccagaaga attttcaatg tcagccgttg acatgtctat gattgaccat
      961 attccagata tgattgaaaa tggtgtggac agtctaaaaa tcgaaggacg tatgaagtct
     1021 attcactacg tatcaacagt aaccaactgc tacaaggcgg ctgtggatgt ctatcttgaa
     1081 agtcctgaaa agtttgaagc tatcaaacaa gacttggtgg acgagatgtg gaaggttgcc
     1141 caacgtgaat tggcaacagg tttctactac ggtataccat ctgaaaatga gcagttgttt
     1201 ggtgctcgcc gtaaaattcc tgagtacaag tttgtcgctg aagtggtttc ttatgatgat
     1261 gcggcacaaa cagcaaccat tcgtcaacga aacgtcatta acgaagggga ccaagttgag
     1321 ttttatggtc caggtttccg tcattttgaa acctatattg aagatttgca tgatgccaaa
     1381 ggcaataaaa tcgaccgcgc tccaaatcca atggaactat tgactattaa agtcccacaa
     1441 cccgttcaat caggagacat ggttcgtgca ttaaaagaag gactcatcaa tttttataag
     1501 gaagatggaa ccagcgtcac agttcgagct taagaaagga aaaggaaatg atagaggcac
     1561 agggtttctt agtggataag caaacaagat gcattcatta ccatagcaag ttggatatta
     1621 ttgctttaca atgctatgat tgtaaaaagt attatgcttg ttatcggtgt cataattcat
     1681 tagaaaatca cccttttgag ccttatccct tatctttgat acaggataag cctattttat
     1741 gtggtgtttg tctaaaacta ttaacatata agcaatataa agaaagctta agttgcctct
     1801 tttgtttttc tcgctttaat ccaggttgcc aaaatcataa ggaacgctat tttaaatagc
     1861 aaatcatcta gttttgaagt aggagaaaac tcaattccaa gagaaaatga agtaaatctt
     1921 cccacaataa aacgcataat atcaagattg ttcaatacct gatactatgc gtttttaaga
     1981 ttttaaagac ttttttccta gccccttttt actttatctg gtattttgac tacttgttaa
     2041 aactggggta attttcgact gtttaacagt tattatgcaa agtctaaaag gttagaattg
     2101 tcaaaacaat ccgtctagag tatgcgtgat gccaaccgtg gtggatgttc tcagtcatgc
     2161 cgttggaagt acgaccttta cgatatgcca tttgggaaag aacgtaagag tttgcagggt
     2221 gagattccag aagaattttc aatgtcagct gttgatatgt ctatgattga ccatatctca
     2281 gatatgattg aaaatggtgt ggacagtcta aaaatcgaag gacgtatgga gtctattcac
     2341 tatgtattaa cagtaaccaa ctgctacaag gcggctgtgg atgcctatct tgaaagtcct
     2401 gaaaagtttg aagctatcaa acaagacttg gtggacgaga tgtggaaggt tgcccaacgt
     2461 gaactggcta caggatttta ctatggtaca ccatctgaaa atgagcagtt gtttggtgct
     2521 cgtcgtaaaa tccctgagta caagtttgtc gctgaagtgg tttcttatga tgatgcggca
     2581 caaacagcaa ctattcgtca acgaaacgtc attaacgaag gggaccaagt tgagttttat
     2641 ggtccaggtt tccgtcattt tgaaacctat attgaagatt tgcatgatgc caaaggcaat
     2701 aaaatcgacc gcgctccaaa tccaatggaa ctattgacta ttaaggtgcc tcaacctgtt
     2761 caagctggag acatggttcg tgcattaaaa gaaggactca tcaatcttta taaggaagat
     2821 ggaaccagcg tcacagttcg tgcttaatgt agttgtttag ttttaaaaaa ttatgcaaag
     2881 ctccatatac aacacttaaa cgagattaaa gaatggcgaa atcccttgat gcgcaagaga
     2941 ttagctgtcc ttttttattt tttaagtgat aaagtcggag cttaggtatc aaagcctatc
     3001 aaattaaaca aagaagtgat gtcttagata ttttgaaaaa aattaataag cagaaaactc
     3061 tctattattt tgttgtagag agttttttgt taataaaatt ttacaaaatg acatttatat
     3121 attgcattaa gttagatata tgatataatg ttgttaaaaa gaggcgcaac tttttaaact
     3181 caataagaat caaagagcaa accatgaata ttaatggagg aataaaaaat gtaagttata
     3241 aatgtaagta agcattatgg tcattcaatc attctcaaag atataaattt tgcacttaac
     3301 aagggtgaaa ttgttggtct agcagggaga aatggagttg gtaagagtac gttgatgaaa
     3361 attcttgttc agaataatca accgacttca ggtaatatta taagcagtga taatgttggg
     3421 tatttaatcg aagaaccaaa attattttta tctaaaacag gtttagagaa tttaaaatat
     3481 ttgtcaaatt tatatggtgt tgactacaat caagaaatat ttagaagttt gatccaagag
     3541 ttagatttga ctcagtctat taataaaaaa gtaaagacct attctttggg tacaaaacaa
     3601 aaattagctt tgcttctaac tctcgttacg gaacctgata tattgatttt agatgaaccg
     3661 actaatggtt tagatattga atcatcacaa atagttttat cggttctaaa aaatttagct
     3721 ttacatgaaa atgtgggaat tttaatatcg agtcataaat tagaagatat tgaagaaatt
     3781 tgtgagaggg ttcttttctt ggagaacggg cttttgacat ttcaaaaagt aggaaaagat
     3841 agtcataatt tcttgtttga gatagctttt tcatcagcta cagatagaga cattttcatt
     3901 accaaacaag aatttgggga tattgttcag gaagagggac tgagcattac tatgtctggg
     3961 aatattcaaa gtagtgagct ttttaaattt tttaacgaaa actctattaa agtagttgat
     4021 tttgaaacta aaaaagagac gcttaaagat atttatctaa atcgttcaaa ataaagga
//
LOCUS       DAVKJU010000094         3970 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVKJU010000094 DAVKJU010000000
VERSION     DAVKJU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.094"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..136
                     /locus_tag="VW191_001927"
     CDS             <1..136
                     /locus_tag="VW191_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEU9624638.1"
                     /translation="ISKYDLKYNPGNYTMSPLAAFAEIEEIYIELSKIGLVKIVNKSN
                     "
     gene            148..2799
                     /locus_tag="VW191_001928"
     CDS             148..2799
                     /locus_tag="VW191_001928"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEU9624639.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNGGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHSIMPFVTEEIFGQYAEGSIVTAAYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ECDKQADYQAKYDATVARIDEMKKLVK"
     gene            2880..>3970
                     /locus_tag="VW191_001929"
     CDS             2880..>3970
                     /locus_tag="VW191_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEU9624640.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQSDN"
BASE COUNT         1208 a          777 c          874 g         1111 t
ORIGIN      
        1 gataagcaag tatgatctta aatataatcc tggaaattat actatgtcac cacttgcagc
       61 atttgctgaa attgaagaga tttatattga actttcaaaa attggtttgg ttaaaattgt
      121 caataaaagt aattaaggag aacacacatg tctaaagaac tttcacctaa atacaatcca
      181 gccgaggttg aggctggtcg ttaccaaaaa tggcttgatg cggatgtttt caagccttca
      241 ggcgatcaaa aggctaagcc ttattcaatc gtgattccac caccaaacgt aactgggaaa
      301 cttcaccttg gtcacgcttg ggatacgact ttgcaggata tcatcatccg tcaaaaacgc
      361 atgcagggct ttgatacgct ttggcttcca ggtatggacc acgctggtat tgcgactcag
      421 gctaaggttg aggagcgctt acgtggtgag ggaatttccc gctatgacct tggtcgtgag
      481 tctttcttga cgaaagtctg ggaatggaaa gacgaatatg ccactactat caaggaacaa
      541 tgggggaaga tggggctctc tgtagactac tctcgtgagc gtttcactct tgacgaaggt
      601 ttgtcaaaag ctgttcgtaa ggtctttgtg gacctttaca agaaaggctg gatctaccgt
      661 ggtgagttta tcatcaactg ggacccagca gctcgcacag ccctttctga tatcgaggtt
      721 attcacaagg atgtggaagg tgccttctac cacatgaatt acatgctgga agatggttca
      781 cgcgcccttg aagttgctac aactcgtcct gagactatgt ttggggacgt tgcggttgcg
      841 gttaatccag aagacccgcg ctacaaggac ttgattggta aaaacgtcat ccttccaatc
      901 gctaataaac tcatcccaat cgttggagat gagcacgcag atcctgagtt tggtactggt
      961 gtcgtgaaaa tcacacctgc ccacgatcca aatgacttct tggttggcca acgtcataac
     1021 ttgccacaag tcaacgtcat gaacgacgac ggaactatga atgagcttgc ctttgaattt
     1081 tcaggcatgg atcgttttga agctcgtaag gcagtcgttg ctaagttgga agaaatcggt
     1141 gcccttgtca aaatcgaaaa acgtgtccac agtgttggtc actcagaacg tacaggtgtc
     1201 gtagttgagc cacgcttgtc tactcaatgg ttcgtcaaga tggaccaatt ggctaagaac
     1261 gccattgcca accaagacac agaggacaag gtcgaattct acccacctcg tttcaacgat
     1321 accttccttc aatggatgga aaatgtccac gactgggtta tctctcgtca gctttggtgg
     1381 ggtcaccaaa tccctgcctg gtacaatgct gatggtgaaa tgtatgtcgg cgaagaagct
     1441 ccagaaggtg acggatggac tcaggacgaa gacgtcttgg atacttggtt cagttctgcc
     1501 ctctggccat tttcaaccat gggctggcct gaagtcgact cagaagactt taaacgttat
     1561 tttccaactt caaccttggt aacaggttac gacatcatct tcttctgggt gtctcgtatg
     1621 atcttccagt cattggaatt cacaggccgt caaccattcc aaaacgtcct tatccacggt
     1681 cttattcgtg acgagcaagg acgcaagatg tctaagtctc tcggtaacgg gattgaccca
     1741 atggatgtta tcgagaaata cggtgccgat gcccttcgat ggttcctttc aaacggttct
     1801 gcgccaggac aagacgtgcg tttctcttat gagaaaatgg atgcttcatg gaactttatt
     1861 aacaagattt ggaacatttc tcgctatatc ctcatgaaca atggaggttt gacgctggat
     1921 gtggcgcatg acaatgtcac aaaagttgca acaggtgagg ctggtaatgt gacggaccgc
     1981 tggattctcc acaatctcaa cgaaaccatt gcaaaagtta ctgaaaactt tgataagttc
     2041 gagtttggtg tggctggtca tatcctttac aacttcattt gggaagaatt tgccaactgg
     2101 tatgttgagt tgaccaagga agttctttac agcgacaatg aagacgataa ggtaattact
     2161 cgatctgttc tcctctatac gctggataaa atccttcgtc tccttcactc aatcatgcca
     2221 ttcgtgacag aagaaatctt tggtcagtac gctgaaggct ctatcgtgac agcagcatac
     2281 ccaactgtca acccagcctt tgaggacctt gctgcccaca ctggtgtgga aagtctcaaa
     2341 gacttgatcc gtgccgttcg taatgcgcgt gcggaagtaa atgtagcacc aagcaaacct
     2401 atcacaatcc ttgttaagac tagcgatagt gacttggaag ccttctttaa cagcaatgtc
     2461 aactacatca aacgctttac aaatccagaa catttggaaa tcgcatcaac catccctgca
     2521 cctgaactgg ctatgtcaag tgtcatcaca ggagcagaaa tctacctgcc actggccgat
     2581 ctcctcaatg ttgaagaaga actggcccgt ctcgacaagg aacttgctaa atggcaaaaa
     2641 gaactggaca tggttggtaa gaaactctct aacgaacgct tcgtagccaa cgccaaacca
     2701 gaagtcgtcc aaaaagaatg cgacaaacaa gctgactacc aagccaagta tgatgcgacc
     2761 gtagcacgta ttgatgagat gaagaagttg gtgaaatgat gttaaaaacc taaccctatc
     2821 gggttgggtt ttacatttga taatgttata ataaaaagga gaaactttag gagaatacta
     2881 tggggaaaat tgttgctatt gatttattta gcggtgcagg tggcactaca agtggcctta
     2941 aaaaatcagg catagatgtt caagttgctg ttgaaattga ttccgtagct gttaaaactt
     3001 ataagttaaa taatcctgag gtgtctgtaa tagataaaga tataaatgta gtatcaggag
     3061 atgaagtaaa agagtatctt aaaattgata cagacgataa actaatgctc gtggcatgcc
     3121 caccatgtca aggtttttca actattggta ctaataatga aaatgacgag aggaatcaac
     3181 ttgtttttca atttcttcgt ttgattaatg aattgaatcc tgattttcta ttaatggaaa
     3241 atgttgtggg tatgactaga acaagaaatg aaaaaatctt taaaaaattt ttgacatcaa
     3301 tcgataataa ttatagtgtt atttatgaca ttttaaatac agctgattat ggagttcctc
     3361 aatctagaaa aagattggtg ttacacggga ttagaaaaga aattcttgat aacacgaatt
     3421 taactttgaa tttgccttcc aaaactcatt caaatatacc agaattggac cttctttcat
     3481 ggattaatgc ggatataata atggacttgc ctcctattga aggtggtgaa gaatatattg
     3541 gagaaggtat ctacaatcat gttgcaaaca aactatctaa tttaaatatt gagagaatta
     3601 ctcatattag aaataatggg ggaagtagaa cctctttgcc aaaatcttta gaattaaatt
     3661 gtcataaaaa tcgtttaggt catagtgacg tatatggaat attagatatc aacaaacctt
     3721 ccattactat tacaggaggg tgtatgtcat attcaaaagg tagatttggg cacccaactc
     3781 aaaatagagc tttaagtgca agagaagctg caagattaca gtcttttgat gatgactata
     3841 tttttgttgg aaatagaggt caattagcta agcaaattgg taatgcagtt ccagtaaaat
     3901 tagctcaagc tagtggaaat tattttatgt cagtttatag ggagatagca aatggacaat
     3961 cagataattg
//
LOCUS       DAVKJU010000095         3952 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVKJU010000095 DAVKJU010000000
VERSION     DAVKJU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.095"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            63..1127
                     /locus_tag="VW191_001930"
     CDS             63..1127
                     /locus_tag="VW191_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU9624641.1"
                     /translation="MYYFIKVEKDLKNTAGIKAPDDIEKISEELGMKEIRFPKFPFEK
                     NKVIQKLWLFCVVGYNWISLLWRLKKNDVVIYQHPMYGVRVANFAIPLLKKYKNIKFI
                     SVIHDLESLRKGIQGVIEDNETTNAIADKELLSKFDKVISHNPKMTEYLEGIGIKKEN
                     LVELQIFDYLDPSEIEEKIEDGVVIAGNLAKGKSSYIYKLLENELNFKLNLFGPNFIN
                     EELPENVEYFGSLPPNKLPQKLVGKFGLVWDGDSLETCSGNTGNYLKYNNPHKTSLYL
                     ASGIPVIIWKEAALAQFIEENNVGITVNNLSEIEFVMQNISEGEYLSIKRNTMQLGEK
                     LRNGYFYRQAISKCKNDFMK"
     gene            1292..2095
                     /locus_tag="VW191_001931"
                     /pseudo
     CDS             1292..2095
                     /locus_tag="VW191_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2139..3554
                     /locus_tag="VW191_001932"
     CDS             2139..3554
                     /locus_tag="VW191_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003009066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEU9624642.1"
                     /translation="MKVLKNYAYNLSYQLLVIILPIITTPYVTRVFSSNDLGTYGYFN
                     SIVTYFILLATLGVANYGTKVISGHRKEIEKNFWGIYSLQLGATVFSLTLYCLLCLTL
                     PFMQNPVAYILGLALVSKGLDISWLFQGLEDFRKITVRNITVKFVGVISIFLFVKSAD
                     DLYLYVFLLTIFELLGQLSMWVPAREFIGRPHFDLEYARHHLKPVILLFLPQVAISLY
                     VTLDRTMLGALASTKDVGIYDQALKLVNILLTLVTSLGSVMLPRVSNLLSSGDHKAVN
                     KMHEISFLIYNLVIFPIMAGMLIVNDDFVTFFLGQDFQEARYAIAIMIFRMFFIGWTN
                     IMGIQILIPHNQNKEFMLSTTIPAIVSVGLNLLLLPKFGYIGAAIVSVLTEALVWAIQ
                     LYYIRAYLKDVPIIRTMIKIILASAIMYSVLLVSKTYIHFSPTINVLVFVVLGGIIYL
                     FSVLSLKVVDVIELKQVIRKN"
     gene            3556..>3952
                     /locus_tag="VW191_001933"
     CDS             3556..>3952
                     /locus_tag="VW191_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEU9624643.1"
                     /translation="MHKFKDINLDLLKVIACVGVVLLHTEMGGFKETGSWNFSTYLYY
                     LGTYSIPLFFIVNGYLLLGKKEITYSYILQKVKYILITVLSWTFIVWLFKRDFSVNPI
                     KKLIGSLVQKGYFFQFWFFGALILIYICLP"
BASE COUNT         1314 a          579 c          756 g         1303 t
ORIGIN      
        1 aatgttttga gaatgtttaa gaatattgtg tagttaacaa atattggtat aaggtgatag
       61 aaatgtatta ttttataaaa gtagagaaag atttaaaaaa tacagccggg attaaagcgc
      121 ctgatgatat agaaaaaatt tcagaagaat tgggaatgaa agaaatacgc tttcccaagt
      181 ttccttttga aaaaaataaa gttatacaaa aactctggct tttttgtgtt gtaggttata
      241 attggataag tctgttgtgg agacttaaaa agaatgatgt agttatttac caacatccaa
      301 tgtacggagt gcgtgttgca aattttgcaa ttcctttatt gaaaaagtat aaaaatataa
      361 aatttatttc agtaatacat gatttagaat cgttaagaaa aggaatccaa ggagtcattg
      421 aagataatga aacaacaaat gctattgcag acaaagagtt gttgtcaaaa tttgataaag
      481 tgatttccca taatccgaaa atgacagaat atttagaagg gatagggata aaaaaagaaa
      541 atttagttga attgcaaata ttcgactacc tagatccatc agaaatagaa gaaaagattg
      601 aagatggagt ggttatagcg gggaatctag caaaaggaaa aagttcatat atatataagt
      661 tgttagaaaa tgaactgaac ttcaaattaa atctttttgg gccaaatttt attaacgaag
      721 agctaccaga aaacgttgag tattttggta gtttacctcc aaataaattg cctcaaaagc
      781 tagtaggtaa gtttggtttg gtttgggacg gtgatagtct agaaacttgt agtggaaata
      841 ctggtaacta tttgaagtat aataatccac ataaaacctc attatatctt gcttcaggaa
      901 tacctgtaat tatctggaaa gaagctgcgt tagcacagtt tattgaagaa aataatgttg
      961 gtattactgt aaataatttg tcagaaattg agtttgtgat gcaaaatata tctgagggcg
     1021 aatatttatc aataaaaaga aatacaatgc aattgggtga aaaattaaga aatggatatt
     1081 tttatcgtca ggcgatatct aagtgcaaaa atgatttcat gaagtaagag ggcatataaa
     1141 aagacggaaa atatttagca ataaatactg tagcttattt tttattgaag tggatgtgta
     1201 atttcagata actatggagt tgctcaccaa ttaaatcaga actcaaaaaa caatagactc
     1261 cctgcgaaac aaaatatggt atagtagttc tatgaatgat gaagcaagta aacaactaac
     1321 tgatgcacga tttaagcgtc ttgttggtgt tcagcgcacg acttttgaag agatgttagc
     1381 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
     1441 cctagaagac cttcttatgg ccactcttca atatgtgcga gaatatcgaa cttatgaaga
     1501 aattgcggct gattttggta tccacgaaag caacttactc cgtcggagcc aatgggttga
     1561 agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagtt ctgaggacac
     1621 ggtaatgatt gatgcgacgg aagtacaaat caatcgccct aaaaaaagaa ttagcgaatt
     1681 attctggtaa aaaaaatgcc acgctatgaa ggctcaagcg attgtcacaa gtcaagggag
     1741 aattgtttct ttggatatca ctgtgaacta ttgtcatgat atgaagttgt tcaaaatgag
     1801 tcgcagaaat atcggacaag ctggtaaaat cttggctgac agtggttatc aagggctcat
     1861 gaagatatat cctcaagcac aaacttcacg taaatccagc aaactcagac cgctaatagt
     1921 tgaagataaa gcctgtaacc atgcgctatc taaggagaga agcaaggttg agaatatctt
     1981 tgccaaagca aaaacgttta aaatgttttc aacaacctat cgaaatcatc gtaaacgctt
     2041 cggattacgg atgaatttga ttgctggtat tatcaatcat gaactaggat tctagttttg
     2101 caggaagtct aataattaaa ttggaattgt gaacgtaaat gaaagtactt aaaaactatg
     2161 catacaatct atcttatcaa ttattggtga ttatacttcc aatcattacg accccctatg
     2221 tgacacgggt cttttcttcg aatgatctag ggacatatgg ttattttaac tccatcgtta
     2281 cttattttat cctcttagca acgctagggg ttgccaacta tgggaccaag gtcatttcag
     2341 ggcatcgcaa agaaattgaa aaaaactttt ggggaatcta ctctctgcaa ttaggtgcaa
     2401 cagttttttc tctaaccttg tattgtcttc tttgtctaac tcttcccttt atgcaaaatc
     2461 cagtagccta cattctaggc ttggctttgg tttctaaagg attagatata tcctggcttt
     2521 ttcaagggct agaggatttt cgaaagatta ctgttcgaaa tatcacagtc aagttcgttg
     2581 gtgtaatctc tatcttcctc tttgtcaaat ctgcagatga tctatacctc tatgtctttt
     2641 tgctaaccat atttgaactc ttggggcagc taagtatgtg ggtgcctgct cgtgagttta
     2701 ttggtagacc tcattttgat ttagaatatg ctagacatca tttgaaacca gtcatattat
     2761 tgttccttcc gcaagtggcg atttccttgt atgttacgct agatcgtacc atgcttggag
     2821 cgttagcttc tacaaaagat gtagggattt atgaccaggc tttaaagttg gtaaatatcc
     2881 ttctgacctt ggtaacttcc ttgggaagtg tcatgttgcc tagagtttca aatcttttat
     2941 cctcagggga tcataaagca gttaacaaaa tgcatgagat atcattttta atttataatt
     3001 tggttatttt ccccattatg gcaggaatgc taattgtaaa tgatgacttt gttacctttt
     3061 tcctcgggca agattttcaa gaggcacgtt atgcaatagc aattatgatt tttagaatgt
     3121 tctttattgg ttggaccaat attatgggga ttcaaatttt gatacctcat aatcaaaata
     3181 aagaatttat gctgtcaaca acaattcctg ctattgttag tgttggatta aatcttctct
     3241 tgcttcccaa gtttggatat ataggagcag caattgtttc tgtcttaaca gaggcgctag
     3301 tatgggctat tcaattgtac tatattcgtg catacctaaa agatgttccg attattagaa
     3361 ctatgataaa gattattcta gcctcagcta tcatgtatag cgttttgcta gtttctaaga
     3421 catacataca tttttcccca actataaatg ttttggtatt tgtggtgtta ggtggaatca
     3481 tttatctttt ttcagtttta tctctaaaag tggtagatgt gatagaatta aaacaagtaa
     3541 ttaggaaaaa ttagaatgca taaatttaaa gatattaact tggatttact aaaagtaatt
     3601 gcatgtgttg gagttgtttt actccataca gagatggggg gatttaaaga gacaggttca
     3661 tggaattttt cgacatattt atactactta ggaacctatt ctatccctct attttttata
     3721 gttaatggtt atttattgtt agggaagaaa gagattacct attcctacat attacagaaa
     3781 gtaaaatata ttctaataac agtgctgtca tggaccttta tagtttggtt atttaaacgg
     3841 gatttttcag ttaatccaat aaaaaaactt ataggatcat tggtgcaaaa aggttatttc
     3901 tttcagtttt ggttttttgg tgcgcttata cttatttata tatgtctccc cg
//
LOCUS       DAVKJU010000096         3884 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVKJU010000096 DAVKJU010000000
VERSION     DAVKJU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.096"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..245
                     /locus_tag="VW191_001934"
     CDS             <1..245
                     /locus_tag="VW191_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624644.1"
                     /translation="IKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYL
                     LVIQKAGYLFSVLLLFSNFSVPFIKEKEYELFKNSK"
     gene            1378..1881
                     /locus_tag="VW191_001935"
     CDS             1378..1881
                     /locus_tag="VW191_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624645.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLETFKVNFCLTDLHHLF
                     GLHKITRDFASHTISVIQTGVFILEDFRNILLYNDVIERISLYEFIGDIFYSKLTSYC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            2167..>3884
                     /locus_tag="VW191_001936"
     CDS             2167..>3884
                     /locus_tag="VW191_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEU9624646.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAAEAQKKYEDDQKRTEEKGALEEETSRKVNDATLE
                     VQNAYVEYGEANKKKGKDREKKLADAQKKIDEAQEKLTKAKNEFQSVRAMVVPEPEQL
                     AETKKKAEEAKAEEAVAKEKSDKAAKEVEVAKKRVEAEEAELDKKVAELQNKVADLEK
                     EIADAEKTVADLEKEVAKLEKYVEDFKNSNGEQAEQYLAAAEKDLVAKKAELAEAKIK
                     AATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAAEAELNKKVEALQNQVAE
                     LEEELSKLEDNLKDAETNNVEDYIKEGLEEAIATKKAELEKTQKELDAALNELGPDGD
                     EEETPAPAPQPEKPAPAPAPKPEQPAPAPKPEKTDDQQAEEDYARRSEEEYNRLPQQQ
                     PPKAEKPAPAPKPEQPVPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNG
                     AMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANGAMATGWLQYNDSWYYLNA
                     NGAMATG"
BASE COUNT         1424 a          574 c          785 g         1101 t
ORIGIN      
        1 tgataaaaat ttgtgtagat actacgataa aatttttatt catcagatta tttgacacaa
       61 gtactttaat gaagaggaga atattttttc ttttcgcatt ggatgttata gcttacttat
      121 ttttaggata tcttttagtg attcaaaaag caggctattt gttttcggtt ttacttcttt
      181 tttctaattt ttcagttcca tttatcaaag aaaaggaata cgaattattt aaaaatagca
      241 agtaatagaa atatggatta aatcttagaa cagcttaaaa tcagatatga taaagggaaa
      301 tagtcttatc taggtaacta agtgctacaa acacagtcga ggatcacttg ttaggattac
      361 attttaatca tgcttaataa gaatggagaa ttcctatggg gattgtatta ctctgaagtt
      421 ttgaatacct tagttctcgt gattttgagc tattctagct ttagaatcct tcagaacgta
      481 aaattttaag cagtcaaaga cttagaatag tctgaaagca tttattttat agcaattcta
      541 ggcttagaat tgctcatata tatgtatttg agctgctcga tagttaggac agctctattc
      601 tgagaaatct gagctatcaa tgcttcagaa tagtattcgc caatggtttt tattgaagag
      661 gtgactgttt tatgatagtc taaaataaaa ttgatttaat agagtaaatg gagaatcgtt
      721 acatgaaaca tttttttgca ggaattggtg agataaaact tgttggctac ttattgtata
      781 tatgtgtagg actagcccct ctttttcata tatatgtcat aggttcggaa ctgagttttg
      841 tgaaaatagt gttatctatt ttgggagtta tatttgtaag tatgttaact attgctcgaa
      901 tttatcggac ttttctataa agaatagttt ggacttgaac tgtatctaaa aacagaggtt
      961 ggtcgattat cagcctcttt cttgtcgttg aaaagagtga gatatattga cttttgaaaa
     1021 aaagatgtca taattgagtt gggataaacc taaaagtaat atagtagatt gaatctggaa
     1081 tagtccgtta tgactgttaa aatatttcta gaaattaatt tgactttcct aatcgatttg
     1141 ttcacatctt gtttcaatct actatacttt ctcgttaaac cgttcggcaa ttcatggtga
     1201 catggacaca tagtagtaag cacactatgt ggcttggcag agctaaaaac tgttcccttg
     1261 cgataagcct aataagcaca acatagggag ttagagaagc cgactctaat caatcacttt
     1321 gggcagtagt gagaactgcc ccgtgctttt tattttgaga aaatatggag tttgtcgttg
     1381 aaattacttg attgtatttt ggattatcaa gaaagattca atggaaaaac atgtcaagta
     1441 tcaacgaatt ataagtattt agagactttc aaagtgaatt tttgcttgac tgatttacat
     1501 catttatttg gcttacacaa aatcacacga gattttgcta gtcatacgat atctgttatt
     1561 caaactggtg tttttatttt ggaagatttt agaaatatcc tattgtacaa tgatgtaatc
     1621 gaaaggatat ctttatatga gtttatcggc gatatcttct attctaagct aacaagttat
     1681 tgcatcgtag ctaaggattt atctaaaaat actatgaaat tggacgtcat attttttgag
     1741 gataaaaata aaagatccgc agttttaggt ttacgaagag ataaaagcgg agtatttaaa
     1801 ccagttactc tacattttac aagcgctaag aaatatgcta aagttcgtaa aacagatgtg
     1861 aaagaaatga aatggctata aagattgtcc gcaggcttaa gcttgcgttt tttgttgttt
     1921 aacgaaatta aaaaacaagc tctctcatcg gaaatgtttt tataggaatg aaggaagatg
     1981 atgctatgtt taaaaagaga atcgaaaagg gcaaaagtag tgtctttatc tttttggagt
     2041 agaagcttat gatatagaaa tttgtaataa aaatgtaata taaaacattt gacaaatatt
     2101 tacgggggga gtatacttaa tatagttata gtctaaaaat gactatcaga aaagaggtaa
     2161 atttagatga ataagaaaaa aatgatttta acaagtctag ccagcgtcgc tatcttaggg
     2221 gctggttttg ttacgtctca gcctactttt gtaagagcag aagaagctcc acaagttgtc
     2281 gaaaaatctt cattagagaa gaaatatgag gaagcaaaag caaaagctga tactgccaag
     2341 aaagattacg aaacggctaa aaagaaagca gcagaagctc agaaaaagta tgaagatgat
     2401 cagaagagaa ctgaggagaa aggcgcgcta gaagaagaaa cttctaggaa ggtaaacgat
     2461 gctactttag aggtacaaaa cgcatatgta gaatatggag aagctaataa gaaaaaaggt
     2521 aaggaccgtg agaaaaaatt agcagatgcg caaaaaaaaa tagatgaagc gcaggaaaaa
     2581 ctaaccaaag ctaaaaatga gtttcaatct gttcgagcaa tggtagttcc tgagccagag
     2641 cagttggctg agactaagaa aaaagcagaa gaagctaaag cagaagaggc agtggctaag
     2701 gaaaaatctg ataaggcagc taaagaggta gaagtagcga aaaaaagagt agaggcagaa
     2761 gaagctgagt tggataaaaa agttgctgaa cttcaaaata aagttgctga tttagaaaaa
     2821 gaaatagctg atgctgaaaa aacggtagca gatttagaaa aagaagttgc taaacttgaa
     2881 aaatatgtag aagatttcaa aaactcaaac ggtgagcaag ctgaacaata ccttgctgca
     2941 gctgaaaaag acttagttgc taaaaaagct gaattagcag aagctaaaat taaagctgct
     3001 actaaaaaag ctgaattaga acctgaatta gaaaaagcag aagctgaact tgaaaaccta
     3061 ttatctacac tagaccctga aggtaagact caggatgaat tagataaaga agctgctgaa
     3121 gctgagttga ataaaaaagt tgaagctctt caaaaccaag ttgctgaatt agaagaagaa
     3181 ctttcaaaac ttgaagataa tcttaaagat gctgaaacaa acaacgttga agactacatt
     3241 aaagaaggtt tagaagaagc tatcgcgact aaaaaagctg aattggaaaa aactcaaaaa
     3301 gaattagatg cagctcttaa tgagttaggc cctgatggag atgaagaaga gactccagcg
     3361 ccggctcctc aaccagaaaa accagctcca gctccagcac caaaaccaga gcaaccagct
     3421 ccagccccaa aaccagaaaa aacagatgat caacaagctg aagaagacta tgctcgtaga
     3481 tcagaagaag aatataaccg cttgccccaa cagcaaccgc caaaagcaga aaaaccagct
     3541 ccagcaccaa aaccagagca accagttcct gcaccaaaaa caggttggaa acaagaaaac
     3601 ggtatgtggt acttctacaa tactgatggt tcaatggcta caggttggct ccaaaacaac
     3661 ggttcatggt actacctcaa cagcaatggc gctatggcta caggttggct ccaatacaat
     3721 ggttcatggt attacctaaa cgctaacggc gctatggcta caggttgggc taaagtcaac
     3781 ggttcatggt actacctcaa cgctaatggt gctatggcta caggttggct ccaatacaac
     3841 gattcatggt attacctaaa cgctaacggc gctatggcga cagg
//
LOCUS       DAVKJU010000097         3837 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVKJU010000097 DAVKJU010000000
VERSION     DAVKJU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.097"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..378
                     /locus_tag="VW191_001937"
     CDS             <1..378
                     /locus_tag="VW191_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9624647.1"
                     /translation="KEHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEA
                     EDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLTGLKSSLLLGTKDN
                     NTISAEVDSLLALLKESQPTPIQ"
     gene            588..3707
                     /gene="phtE"
                     /locus_tag="VW191_001938"
     CDS             588..3707
                     /gene="phtE"
                     /locus_tag="VW191_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEU9624648.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPGN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEINLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKVQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNSE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1437 a          624 c          744 g         1032 t
ORIGIN      
        1 aaggaacatg atgaagtaag tgagccaact caccctgaat ctgatgaaaa agagaatcac
       61 gctggtttaa atccttcagc agataatctt tataaaccaa gcactgatac ggaagagaca
      121 gaggaagaag ctgaagatac tacagatgag gctgaaattc ctcaagtaga gcattctgtt
      181 attaacgcta agatagcaga ggcggaggcc ttgctagaaa aagtaacaga ttctagtatt
      241 agacaaaatg ctatggagac attgactggt ctaaaaagta gtcttcttct cggaacgaaa
      301 gataataaca ctatttcagc agaagtagat agtctcttgg ctttgttaaa agaaagtcaa
      361 ccgactccta tacagtagta aaatgaatgg agcatatttt atggagaagt aacctttcgt
      421 gttacttctc ttttttagaa aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta
      481 aactatttac tggttaatta aatggttaaa taaccggttt agaaaactat ttaataaagt
      541 aaaagaagtt gagaaaaaac ttcatcattt attgaaatga gggatttatg aaattcagta
      601 aaaaatatat agcagctgga tcagctgtta tcgtatcctt gagtctatgt gcctatgcac
      661 taaaccagca tcgttcgcag gaaaataagg acaataatcg tgtctcttat gtagatggca
      721 gccagtcaag tcagaaaagt gaaaacttga caccagacca ggttagccag aaagaaggaa
      781 ttcaggccga gcaaattgta atcaaaatta cagatcaggg ctatgtaacg tcacacggtg
      841 accactatca ttactataat gggaaagttc cttatgatgc cctctttagt gaagaactct
      901 tgatgaagga tccaaactat caacttaaag acgctgatat tgtcaatgaa gtcaagggtg
      961 gttatatcat caaggtcgat ggaaaatatt atgtctacct gaaagatgca gctcatgctg
     1021 ataatgttcg aactaaagat gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg
     1081 agaaggttaa ctctaatgtt gctgtagcaa ggtctcaggg acgatatacg acaaatgatg
     1141 gttatgtctt taatccagct gatattatcg aagatacggg taatgcttat atcgttcctc
     1201 atggaggtca ctatcactac attcccaaaa gcgatttatc tgctagtgaa ttagcagcag
     1261 ctaaagcaca tctggctgga aaaaatatgc aaccgagtca gttaagctat tcttcaacag
     1321 ctagtgacaa taacacgcaa tctgtagcaa aaggatcaac tagcaagcca gcaaataaat
     1381 ctgaaaatct ccagagtctt ttgaaggaac tctatgattc acctagcgcc caacgttaca
     1441 gtgaatcaga tggcctggtc tttgaccctg ctaagattat cagtcgtaca ccaaatggag
     1501 ttgcgattcc gcatggcgac cattaccact ttattcctta cagcaagctt tctgccttag
     1561 aagaaaagat tgccagaatg gtgcctatca gtggaactgg ttctacagtt tctacaaatg
     1621 caaaacctaa tgaagtagtg tctagtctag gcagtctttc aagcaatcct tcttctttaa
     1681 cgacaagtaa ggaactctct tcagcatctg atggttatat ttttaatcca aaagatatcg
     1741 ttgaagaaac ggctacagct tatattgtaa gacatggtga tcatttccat tacattccaa
     1801 aatcaaatca aattgggcaa ccgactcttc caaacaatag tctagcaaca ccttctccat
     1861 ctcttccaat caatccagga acttcacatg agaaacatga agaagatgga tacggatttg
     1921 atgctaatcg tattatcgct gaagatgaat caggttttgt catgagtcac ggagaccaca
     1981 atcattattt cttcaagaag gacttgacag aagagcaaat taaagctgcg caaaaacatt
     2041 tagaggaagt taaaactagt cataatggat tagattcttt gtcatctcat gaacaggatt
     2101 atccaggtaa tgccaaagaa atgaaagatt tagataaaaa aatcgaagaa aaaattgctg
     2161 gcattatgaa acaatatggt gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg
     2221 cgattattta tccgcatgga gatcatcatc atgcagatcc gattgatgaa cataaaccgg
     2281 ttggaattgg tcattctcac agtaactatg aactgtttaa acccgaagaa ggagttgcta
     2341 aaaaagaagg gaataaagtt tatactggag aagaattaac gaatgttgtt aatttgttaa
     2401 aaaatagtac gtttaataat caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt
     2461 ttagttttcc gcctgaattg gagaaaaaat taggtatcaa tatgctagta aaattaataa
     2521 caccagatgg aaaagtattg gagaaagtat ctggtaaagt atttggagaa ggagtaggga
     2581 atattgcaaa ctttgaatta gatcagcctt atttaccagg acaaacattt aagtatacta
     2641 tcgcttcaaa agattatcca gaagtaagtt atgatggtac atttacagtt ccaacctctt
     2701 tagcttacaa aatggccagt caaacgattt tctatccttt ccatgcaggg gatacttatt
     2761 taagagtgaa ccctcaattt gcagtgccta aaggaactga tgctttagtc agagtgtttg
     2821 atgaatttca tggaaatgct tatttagaaa ataactataa agttggtgaa atcaatttac
     2881 cgattccgaa attaaaccaa ggaacaacca gaacggccgg aaataaaatt cctgtaacct
     2941 tcatggcaaa tgcttatttg gacaatcaat cgacttatat tgtggaagta cctatcttgg
     3001 aaaaagaaaa tcaaactgat aaaccaagta ttctaccaca atttaaaaga aataaagtac
     3061 aagaaaactc aaaacttgat gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa
     3121 aagaaaaact ttctgaaact gggaatagta ctagtaattc aacgttagaa gaagttccta
     3181 cagtggatcc tgtacaagaa aaagtagcaa aatttgctga aagttatggg atgaagctag
     3241 aaaatgtctt gtttaatatg gacggaacaa ttgaattata tttaccatcg ggagaagtca
     3301 ttaaaaagaa tatggcagat tttacaggag aagcacctca aggaaatggt gaaaataaac
     3361 catctgaaaa tggaaaagta tctactggaa cagttgagaa ccaaccaaca gaaaataaac
     3421 cagcagattc tttaccagag gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg
     3481 ataatggaat gttgaattca gaagggaatg tggggagtga ccctatgtta gatccagcat
     3541 tagaggaagc tccagcagta gatcctgtac aagaaaaatt agaaaaattt acagctagtt
     3601 acggattagg cttagatagt gttatattca atatggatgg aacgattgaa ttaagattgc
     3661 caagtggaga agtgataaaa aagaatttat ctgatctcat agcgtaagga atagcagtag
     3721 aaaaagtctg aatcaaaaat gaagttctct caaaagttag aaataaaact ctgactttgg
     3781 gagaatttca ttttattatt aatatataaa atttcttgac atacaactta aaaagag
//
LOCUS       DAVKJU010000098         3762 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVKJU010000098 DAVKJU010000000
VERSION     DAVKJU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.098"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            64..282
                     /gene="nrdH"
                     /locus_tag="VW191_001939"
     CDS             64..282
                     /gene="nrdH"
                     /locus_tag="VW191_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEU9624649.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            363..2522
                     /gene="nrdE"
                     /locus_tag="VW191_001940"
     CDS             363..2522
                     /gene="nrdE"
                     /locus_tag="VW191_001940"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEU9624650.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIEIAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2710..3672
                     /gene="nrdF"
                     /locus_tag="VW191_001941"
     CDS             2710..3672
                     /gene="nrdF"
                     /locus_tag="VW191_001941"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEU9624651.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
BASE COUNT         1164 a          810 c          703 g         1085 t
ORIGIN      
        1 attgacaaaa ttttattttc tgatatacta agatagtatt aaattttgaa gaggagttac
       61 acaatggtaa ccgtttattc taaaaacaat tgtgtccaat gtaaaatgac caagcgtttc
      121 ttggacagta ataatgtctc ttatcgtgaa atcaatcttg atgagcaacc tgagtacgtc
      181 gatcaagtta aagagctcgg ttttagcgca gctcctgtta tccaaacacc aactgaagtc
      241 ttttcaggtt tccaaccagg aaaactgaaa caattagcat aatcttagta catcatccag
      301 aagaaactgc ttttagggct aacttagaag cctttctttt gtaattagat aagggaaatt
      361 ttatgggatt aaaacatctt gaagacgtga cttacttccg tcttaataac gaaattaacc
      421 gtcctgttaa tggacaaatc atgcttcata aagacaagga agccttggat gctttcttta
      481 aagaaaatgt agttccaaac actatggttt ttgattcaat caaagacaaa atcaattacc
      541 tcattgaaca caactacatc gaaatagcct ttatcaagaa ataccgtcca gagttcttgg
      601 aagaattggc tcaatttatc aaagaccaaa acttccaatt caagtcattc atggcagctt
      661 ataaatttta caatcaatat gccttaaaga ctaatgacgg tgaatactat cttgaaaata
      721 tggaagaccg tgtcttcttc aacgcccttt atttcgctga tgggaatgaa gctgttgcaa
      781 tcgatattgc caatgaaatc atccaccaac gctaccaacc tgctactcct tccttcttga
      841 atgctggacg tgctcgtcgt ggggagttgg tatcttgttt cctaatccaa gtgacggatg
      901 atatgaactc tatcggacgt tctatcaact cagctcttca actttcacgt attggtggtg
      961 gtgtgggaat taccctcagc aaccttcgtg aagctggtgc acctatcaaa ggctatgaag
     1021 gagctgcttc aggtgtcgtt cctgttatga agctttttga agacagcttc tcttactcca
     1081 accaattggg gcaacgtcaa ggtgctggtg ttgtctacct caacgtcttt cacccagata
     1141 tcatcgcttt cctttcaact aagaaagaaa acgctgatga aaaagtacgt gtcaagactc
     1201 tatcacttgg tgttgtagta cctgataaat tctacgaatt ggctcgtaaa aatgaagaaa
     1261 tgtacctctt cagcccttac tctgtagaaa aagaatacgg tgtgccattc aattacatcg
     1321 acattactga aaaatacgat gaattggtcg caaatccaaa tattcgcaag acaaaaatca
     1381 aggcgcggga tttggaaact gaaatctcta aattgcaaca agagtctggc tatccttatg
     1441 tagtcaacat cgatacggct aaccgtgcaa atcctgttga tggaaagatt atcatgagta
     1501 acttgtgttc tgagattctt caagttcaag aaccaagctt gatcaacgat gctcaagaat
     1561 tccttcaaat gggaacagat gtttcatgta acctgggttc aaccaacgtg gttaacatga
     1621 tgacttcacc tgattttggt cgttctatcc gtgctatggt tcgtgccctt actttcgtta
     1681 cagatagttc acatatcgta gctgtaccaa cgattgacca tggaaatagc caagcccaca
     1741 cctttggtct cggtgccatg ggactgcaca gctaccttgc ccaacaactc attgaatatg
     1801 gttcacctga gtctgttgaa tttacaagca tctactttat gcttatgaac tactggacct
     1861 tggtagagtc aaacaatatt gcacgtgaac gtggtattac cttccacaac tttgaaaaat
     1921 cagactatgc taacggaagt tactttgaca agtatgtaac tggtgaattt gttccaacat
     1981 cagaccgtgt taaagaactc ttcaaaaatg tctttatccc tggtgttgct gattgggctg
     2041 aacttcgcga caaggttcaa gaagatggtc tttaccacca aaatcgcctt gctgtagcgc
     2101 caaatggttc tatcagctat atcaacgacg tttctgcttc tatccacccg attacgcaac
     2161 gtatcgaaga acgccaagag aagaaaattg gtaaaatcta ctaccctgct gctggcttgt
     2221 ctacagaaac cattccttac tacacttctg cctacgatat ggatatgcgt aaagtcatcg
     2281 atgtttacgc tgctgcgact gaacacgtgg atcaaggact ttcactcacc ctcttcatgc
     2341 gcagtgacat tccaaaaggc ctttacgaat ggaagaggga aaataaacaa acgacacgtg
     2401 atttgtccat ccttcgtaac tatgccttta acaagggaat caagtctatc tactacgtcc
     2461 gtacctttac agatgacggt ggagaagtcg gcgccaacca atgtgaaagc tgtgtgattt
     2521 agtttctagc atagaaacat tatttttccc taagcagcac tgataaaatc aaatctacta
     2581 tgtcataaca taatttatgt aacattttaa aagtcgggct tccgactttt tgtgcgatac
     2641 tcctatagaa ttatggtaaa gtagaaataa ttgtgagttc gcaatactaa acacagaaag
     2701 agattttaaa tggaaactta ctacaaagcc attaactgga atgccatcga agatgtcatc
     2761 gacaaatcaa cttgggaaaa actgacggag caattttggc tcgatacacg tattccctta
     2821 tcaaacgact tggatgactg gagaaagcta tctaacaaag agaaagactt ggtcggaaaa
     2881 gtttttggtg gtttaaccct tcttgacact atgcaatctg aaactggggt tcaagccctt
     2941 cgcgcagaca tccgtacacc acatgaggaa gctgtcttca ataacatcca atttatggaa
     3001 tctgtccacg ctaaatctta ctcatcaatc ttttctacct tgaatactaa ggctgagatt
     3061 gaagaaattt tcgaatggac caataccaat ccttacctac aaaagaaggc tgagattgtc
     3121 aacgaaatct acctaaacgg cagcccactt gaaaagaaag ttgccagcgt cttcctcgaa
     3181 accttcctct tctactctgg tttcttcact cccctctact atctcggtaa caacaaactt
     3241 gccaacgttg cagaaatcat taaattgatt attcgtgacg agtctgttca cggaacctac
     3301 attggttaca aattccaact tggtttcaat gaattacctg aagaagagca agaaaaactc
     3361 aaagaatgga tgtacgacct actctatact ctttatgaaa atgaagaagg ctacacagag
     3421 agtctttatg acggtgttgg ttggactgaa gaggttaaaa ccttccttcg ctacaatgcc
     3481 aataaagctc tcatgaacat gggacaagat ccacttttcc cagattcagc tgaagatgtc
     3541 aacccaatcg ttatgaacgg tatttcaaca ggaacttcca accatgactt cttctctcaa
     3601 gtcggaaatg gttaccttct tggcgaagtt gaagccatgc aagatgatga ctacaactac
     3661 ggtttggact aatactcttc gaaaatcaaa ttcaaaccac gtcagcttcg ccttgccgta
     3721 ctcaagtaca gcctgcggct agcttcctag tttgctcttt ga
//
LOCUS       DAVKJU010000099         3700 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVKJU010000099 DAVKJU010000000
VERSION     DAVKJU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.099"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            124..1533
                     /locus_tag="VW191_001942"
     CDS             124..1533
                     /locus_tag="VW191_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEU9624652.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1551..2846
                     /locus_tag="VW191_001943"
     CDS             1551..2846
                     /locus_tag="VW191_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU9624653.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            2851..3159
                     /locus_tag="VW191_001944"
     CDS             2851..3159
                     /locus_tag="VW191_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9624654.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3156..3464
                     /locus_tag="VW191_001945"
     CDS             3156..3464
                     /locus_tag="VW191_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEU9624655.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1252 a          621 c          711 g         1116 t
ORIGIN      
        1 gtataacaaa gttcaagaaa tgaggcaaac acaagatatg tatcaacaat ttttaaaaat
       61 gtctagacaa attttaaaat tatgctatac tttatgtata gaataataga aaggaggttg
      121 agcatgaaaa aaaagttagt atttcctaat ctgttttggt ggggagcagc ttctagcgga
      181 cctcagacag aaggtcaata tggaaaagta catgaaaatg tgatggacta ctggttcaaa
      241 acgcatccag aagatttttt cgataatgtc ggacctcttg tagccagtaa cttttttcat
      301 acttacaccg aagatttcca cttgatgaag gaaattggag ttaattcttt ccgcacttcc
      361 atccaatgga gtcgactcat caagaattta gagacaggtg agcctgatcc aaaaggtatt
      421 gctttctaca atgccatcat tgaagaagct aaaaagaacc agatggatct tgtgatgaat
      481 ttacatcatt ttgatttacc agtggaactt cttcaaaaat acggtggttg ggaaagcaaa
      541 catgtagtgg agttattcgt gaagtttgcc aagactgctt tcacatgctt tggagataag
      601 gttcattact ggacaacttt caatgagcca atggtcattc cagaagcagg atacttatat
      661 gctttccatt atccaaatct aaaaggaaag ggaaaagagg ccgtacaagt catctataat
      721 ctaaaccttg ctagtgcaaa agtgattcaa ctatatcgct cattagaact tgatggaaag
      781 attgggatta ttttaaactt gacacctgct tatccaagaa gtaattctcc agaagactta
      841 gaagcaagtc gatttacaga tgacttcttt aacaaagtct tcttgaatcc agctgttaaa
      901 ggaactttcc cagaaagatt ggtaaaacag ctagagagag atggcgtgtt atggagtcat
      961 accgaaaaag agcttcaact gatgaaatca aatacggttg attttcttgg agtaaactac
     1021 taccatccaa aacgtgttca agcacaagca aatcctgagg aatatcagac gccctggatg
     1081 ccagaccaat acttcaaaga gtatgaatgg ccggagcgtc gcatgaatcc atatcgtggt
     1141 tgggaaattt ttccgaaagc catttatgat attgctatga ttgtgaagga agaatatggt
     1201 aatatcccat ggtttatcag tgaaaacgga atgggtgttg aaaacgaagc acggtttatc
     1261 gatgaaaatg gagttatcga tgacgtgtat cgtattgaat tttatgaaga acatttaaga
     1321 tggctacata aagccattga agagggaagt cactgttttg gataccacgc ttggaccgca
     1381 tttgattgct ggtcttggaa taatgcatat aagaatcgtt acggatttat ctccgttgat
     1441 ttagaaacgc aaaagagaac catcaagagc tcaggaagat ggtatcgcaa agtaagtgac
     1501 aataacggtt ttgaagtaga aattgaggag taaagagagg agatattcat atgaaaaact
     1561 caaaatttat agaccaattt gccacctttg ctggtaaact agggaaccaa attcatttaa
     1621 aaaccctaag agatgcattc gtaacagtaa tgccattata tattttggca ggtttaatcg
     1681 ttcttttgaa caacacggta tttaagtgga ttttccaagg ggatacatta acaagattcc
     1741 aatattgggg aataacaatt gcaaacggta ctttaagtat ttcaggtatg attattgctg
     1801 taatggttgg ttatttctta gctaaaaaca gagatttcga aaacccgtta gcagcatcaa
     1861 tgctatcatt agtttcttta attgtgatga tgccaaatac agtttctgta gttcctgacg
     1921 gagcaaaaga tgctgtaaac atttcaggtg ttctttcatt caacaacaca ggtacaggcg
     1981 caatgttcgc cggggttatc gtagcgatta ttgcaacaga attattcatt gaattatcaa
     2041 atgttaaagc tttacaaatg aatcttggtg aaaatattcc accagctgtt agtagatcat
     2101 ttagcgtatt acttccagtc atgaccgtca tctccttatt tggggttgtt tcagcattat
     2161 tattcaatat aactggaatg aacttaatct caatcattac aatctttatt caagaaccaa
     2221 ttcgtcatat tggtacaagc ttaatcgggg tcattattat ttactcttta ggaaatatgt
     2281 tatggctatt tggtattcac caagcagtta tttacagtgc catcctagaa ccattactat
     2341 taattaacat tactgaaaac atcactgcag caaataatgg acaagccatt ccacacatca
     2401 tcaacctatc acaaatacaa acattcgctt taatgggtgg tagtggatct acattatgtt
     2461 tattaatagc aacattctta gtgagtcgca atgctgtctc taaaaacgtg gctaaattat
     2521 cttttggacc tggtatcttc aatatcaatg aaccagtatt attcggttac ccaatcgttt
     2581 ataacatttc attagctatt ccatttatca cagttccagt ccttggtatt ttaatcagct
     2641 acttagcaac agttacagga ttcatgagtc ctgcatttat acaagttcct tggactacac
     2701 cagtattctt aaatgcatgg ttagcaacag caggggacgt gagagcagtt ctagttcaat
     2761 tcatcatctt tgcacttgga gttcttctat acattccatt tatcaaagtt aatgacaaag
     2821 ttgttgaaca agaaatggaa ggttaagatt atgaaacaaa ttttattagt atgtaatgcg
     2881 ggaatgtcga caagtatgct tgttaaaaaa atgcaacaaa gtgcgactga acgtgggatt
     2941 gaaatctcta tccaagcaaa atcaatgaca gaagctaaaa agaatatcca tgaagcagat
     3001 gtcattctaa ttgggccaca aattcgttat gagttacttg ctgtgaaaga aattgcagga
     3061 aatatccctg tagataccat tgatatgcgg gattacggaa tgatgaatgg agcaaaagtt
     3121 ctggaacaag cacttgcatg gataggagaa ataagatgac agaagaaatg gaatatatct
     3181 gttttcagct cattgccaat agtggagcag ccaaatcatc ctttattgaa gccatccaac
     3241 tagctaaggc aggaaattta aaagaagcga aaattaaagt tgaagaagcg gaagactcct
     3301 tagtagaagc gcacaagatt cactctaatc tcatacaaaa agaagctact ggagaaaaaa
     3361 taggattctc cttgctattt atgcatgcag aagaccaaat ggcttcaaca gagattattc
     3421 agttgctatc taaagaattc atcgatctct atcaaaataa ataggaacac tgtcattccc
     3481 atctgtcttt attaatgacg actacattgt aggaacctcc aaaagttgga caaaatgatt
     3541 gaaaggattg gatcctgcat tacacaagat ccaatccttt tcttttactc tagtttttat
     3601 aatatgttga atctagaata gtccactgta gtttctaaaa aattgctgga aatggatgtt
     3661 aagctccaat ttatttgttt atatcttatt tcagtttact
//
LOCUS       DAVKJU010000100         3647 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVKJU010000100 DAVKJU010000000
VERSION     DAVKJU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.100"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            176..1405
                     /gene="arcA"
                     /locus_tag="VW191_001946"
     CDS             176..1405
                     /gene="arcA"
                     /locus_tag="VW191_001946"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEU9624656.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            1463..2479
                     /gene="argF"
                     /locus_tag="VW191_001947"
     CDS             1463..2479
                     /gene="argF"
                     /locus_tag="VW191_001947"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU9624657.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            2644..3591
                     /gene="arcC"
                     /locus_tag="VW191_001948"
     CDS             2644..3591
                     /gene="arcC"
                     /locus_tag="VW191_001948"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU9624658.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         1156 a          713 c          759 g         1019 t
ORIGIN      
        1 tctatgtatt ctatcctatg ttatgtataa taacataaaa ttgtgctaaa catcataatt
       61 tatattattt tccgagaaat tatagccatt gacattacct aggaattgcg ttataacata
      121 catgtaagcg gtacccaaaa tggtgccaag tcaaaatttt taaggaggaa aatacatgtc
      181 ttctcatcca attcaggtct tctcagaaat tgggaaactg aaaaaagtta tgttgcaccg
      241 tccaggcaag gagttagaaa acttgttgcc ggactatctt gaaaggcttc tttttgatga
      301 tattcctttc ttggaagatg ctcaaaaaga acatgatgca tttgcccaag ctcttcgcga
      361 tgaaggaatt gaggttctct acctagaaca actcgctgct gaatcattga cctctccaga
      421 aatccgcgat caatttatcg aggaatactt agacgaagcc aacatccgtg atcgtcaaac
      481 caaggttgct attcgtgaat tgcttcacgg catcaaggac aaccaagaat tggttgaaaa
      541 aacaatggct gggattcaaa aagttgaatt gccagaaatt cctgacgaag ctaaagatct
      601 aactgactta gttgaatcag agtatccatt tgcaattgac ccgatgccaa acctctattt
      661 cactcgcgac ccatttgcaa caattggaaa cgccgtatcg cttaaccaca tgtttgcaga
      721 tactcgtaac cgtgaaacac tctacggtaa gtatatcttc aaataccacc caatctatgg
      781 cggaaaagtg gatttggtct acaaccgtga agaagatacg cgtatcgaag gtggagacga
      841 gctaattctt tctaaagacg tccttgcagt aggtatctct caacgtacag acgcagcttc
      901 tatcgaaaaa cttttggtca acatcttcaa gaaaaatgtt ggcttcaaga aagttttggc
      961 ctttgaattt gctaacaacc gtaaattcat gcacttggat actgtcttca ctatggtaga
     1021 ctatgacaag ttcactattc acccagaaat cgaaggcgac cttcacgttt actcagttac
     1081 ttacgaaaac gaaaaactta aaatcgttga agagaaaggt gacttagctg aacttcttgc
     1141 tcaaaacctt ggtgtagaaa aagttcattt gattcgttgc ggtggtggca atatcgtagc
     1201 agctgcgcgt gaacaatgga acgacggttc taacactttg accatcgcac ctggtgtggt
     1261 agttgtttat gaccgcaata ccgtgaccaa taagattttg gaagaatacg ggcttcgctt
     1321 gattaagatt cgcggaagtg aattggttcg gggccgtggt ggacctcgtt gtatgtctat
     1381 gccatttgaa cgtgaagaag tgtaatcgct gttcggcatt cgtcaataga aaatgtaaaa
     1441 aatagaaaga ggaaataata aaatgacaaa ttcagtattc caaggacgca gcttcttagc
     1501 agaaaaagac tttacccgtg cagagttaga ataccttatt ggtctttcag ctcacttgaa
     1561 agatttgaaa aaacgcaata ttcaacacca ctaccttgct ggcaagaata tcgctctcct
     1621 atttgaaaaa acatctactc gtactcgtgc agcctttaca actgcggcta tcgaccttgg
     1681 tgctcaccca gaatacctcg gagcaaatga tattcagttg ggtaaaaaag aatctactga
     1741 agatactgct aaagtattgg gacgtatgtt tgacgggatt gaattccgcg gattcagcca
     1801 acgtatggtt gaagaattgg cagaattctc aggcgttcca gtatggaacg gtctaactga
     1861 cgaatggcac ccaactcaaa tgctcgctga ctacttgact gttcaagaaa acttcggtcg
     1921 cttggaaggc ttgacattgg tatactgtgg tgatggacgt aacaacgttg ccaacagctt
     1981 gctcgtaaca ggtgctatcc ttggtgtcaa tgttcacatc ttctcaccaa aagaactctt
     2041 cccagaaaaa gaaatcgttg aattggcaga aggatttgct aaagaaagtg gcgcacatgt
     2101 tctcatcact gaagatgctg atgaagcagt taaagatgca gacgttcttt acacagacgt
     2161 ttgggtatca atgggtgaag aagacaaatt cgcagaacgt gtagctcttc ttaaacctta
     2221 ccaagtcaat atggacttag ttaaaaaagc aggcaatgaa aacttgatct tcctacactg
     2281 cttgccagca ttccacgata ctcacactgt ttatggtaaa gacgttgctg aaaaatttgg
     2341 tgtagaagaa atggaagtaa cagacgaagt cttccgcagc aagtacgctc gccacttcga
     2401 tcaagcagaa aaccgtatgc acactatcaa agctgttatg gctgcgacac ttggtaacct
     2461 ttatattcct aaagtataat tttagataat aaaccgtcta ccaacagcta tgagggctgc
     2521 gactaatagc tttagtccgg tcctctttta tataatggta atctattatt tcttataaaa
     2581 tatgtgaaaa atcattaaat tgaaatctaa acgcattcta ttgagtgtga taaaggagaa
     2641 tttatggcaa atcgtaaaat tgtagtagct ttgggaggaa atgcgattct ttcttctgac
     2701 ccatcagcaa aggctcaaca agaagcttta gttgaaacag ctaagcatct tgtaaaattg
     2761 attaaaaatg gagatgattt gattatcact cacggtaatg gacctcaagt tgggaatctc
     2821 ttgctccaac atttggcatc agactctgaa aagaaccctg ccttcccact cgactcactt
     2881 gtcgctatga cagaaggtag catcggtttc tggttgaaaa atgctttgca aaatgctctc
     2941 ttggatgaag gcatcgaaaa aaatgttgcc tctgttgtaa cgcaagttat cgtagataaa
     3001 aatgatccag cttttgttaa cttgagtaaa ccaatcggtc ctttctattc agaagaagaa
     3061 gcaaaagcag aagccgaaaa gagcggagcg actttcaagg aagatgctgg ccgtggctgg
     3121 cgtaaggtcg ttgcctcacc aaaacctgtt gacatcaaag aaattgaaac catccgtact
     3181 cttttaaata atggtcaagt cgtcgtagct gcaggtggtg gcggtattcc cgtcgtcaaa
     3241 gaaaacaatg gacatttgac tggtgtcgaa gcggttattg ataaagactt cgcttcccaa
     3301 cgtttggcag aattggttga tgcagacctc ttcatcgttt tgacaggtgt agattatgta
     3361 tttgttaact acaacaagcc aaaccaggaa aaattggaac atgtgaatgt tgcccagctg
     3421 gaagaatata tcaaacaaga tcagtttgca ccaggtagca tgcttccaaa agtagaagca
     3481 gctatcgctt ttgtcaatgg tcgtccagaa ggaaaagcag ttattacttc ccttgaaaat
     3541 ctaggcgcct tgattgaatc tgaaagcgga acaattattg aaaaaggata agttgtttta
     3601 ctaataagat gtattctatt tctagtatct ttatatcaaa ttagaaa
//
LOCUS       DAVKJU010000101         3614 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVKJU010000101 DAVKJU010000000
VERSION     DAVKJU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.101"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            217..480
                     /locus_tag="VW191_001949"
     CDS             217..480
                     /locus_tag="VW191_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEU9624659.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            486..2219
                     /gene="ptsP"
                     /locus_tag="VW191_001950"
     CDS             486..2219
                     /gene="ptsP"
                     /locus_tag="VW191_001950"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU9624660.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            2474..>3614
                     /locus_tag="VW191_001951"
     CDS             2474..>3614
                     /locus_tag="VW191_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEU9624661.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRENLSNLRTYRRQNSDNTPRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLIFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPS"
BASE COUNT         1190 a          708 c          777 g          939 t
ORIGIN      
        1 tgaaatagca atgattatcc tataaattgt ttttgaagaa actatatcct gtgttttgtt
       61 atctaggata gaagattttc agcaagttat ctgaaaggaa attgaaggaa aatccagaaa
      121 atgcttgaaa aaaatcctag aagatggtat aatactaaat tgtaagggtt atcacatata
      181 actcaaaaaa agaaagaaca aaaggagagt caaactatgg cttctaaaga tttccacgta
      241 gtggcagaaa caggtattca cgcacgtcca gcaacattgt tggtacaaac tgctagcaaa
      301 tttgcttcag atatcactct tgagtacaaa ggtaaatcag ttaaccttaa atcaattatg
      361 ggtgttatga gtcttggtgt tggtcaaggt gctgacgtaa ctatctcagc tgaaggtgca
      421 gatgcagatg acgctatcgc tgcaatctca gaaacaatgg aaaaagaagg attggcataa
      481 gggaaatgac agaaatgctt aaaggaatcg cagcatctga cggtgttgca gttgcaaaag
      541 catatctact cgttcagccg gatttgtcat ttgagactat tacagtcgaa gatacaaacg
      601 cagaagaagc tcgccttgat gccgctctac aggcatcaca agacgagctt tctgttattc
      661 gcgagaaagc agtaggtacg ctcggtgaag aagcagctca agtttttgat gctcacttaa
      721 tggttcttgc tgacccagaa atgatcagcc aaatcaagga aactatccgt gcgaagaaag
      781 tgaatgcaga agcaggtctg aaagaagtta cagatatgtt tatcactatc tttgaaggca
      841 tggaagacaa cccatacatg caagaacgcg cagcggatat ccgcgacgtg acaaaacgtg
      901 tattggcaaa ccttcttggt aaaaaattgc caaacccagc ttctatcaat gaagaagtga
      961 ttgtgattgc gcatgacttg actccttcag atacagctca attggacaaa aactttgtaa
     1021 aagcttttgt aaccaacatt ggtggacgta caagccactc agctatcatg gcacgtacac
     1081 ttgaaattgc tgctgtatta ggtacaaaca acatcactga aatcgttaaa gacggtgaca
     1141 tccttgctgt taacgggatc actggagaag tgattatcaa cccaacagat gaacaagcgg
     1201 cagaatttaa agcagctggt gaagcctatg cgaaacaaaa agctgaatgg gcacttttga
     1261 aagatgctca aacagtgact gctgacggta aacacttcga gttggctgcc aatattggta
     1321 ctccaaaaga cgttgaaggt gttaacaaca acggtgcaga agctgttgga ctttaccgta
     1381 cagagttctt gtacatggat tctcaagact tcccaactga agatgagcag tatgaagcat
     1441 acaaggctgt tcttgaagga atgaacggta aacctgttgt cgttcgtaca atggatatcg
     1501 gtggagataa ggaacttcct tacttcgata tgcctcacga aatgaaccca ttccttggat
     1561 tccgtgctct tcgtatctct atctctgaga ctggagatgc tatgttccgc acacaaatcc
     1621 gtgctcttct tcgtgcgtct gttcacggtc aattgcgtat catgttccca atggttgcgc
     1681 tcttgaaaga attccgtgca gcgaaagcag tctttgatga agaaaaagca aaccttcttg
     1741 ctgaaggtgt tgcagttgcg gataacattc aagttggtat catgatcgag attcctgcag
     1801 cggctatgct tgcagaccaa tttgctaaag aagttgactt cttctcaatt ggtacaaatg
     1861 acttgatcca atatacaatg gcagcagacc gtatgaacga acaagtttca tacctttacc
     1921 aaccatacaa cccatcaatc ctacgcttga ttaacaatgt gatcaaagca gctcacgctg
     1981 aaggtaaatg ggctggtatg tgtggtgaga tggctggtga ccaacaagct gttccacttc
     2041 ttgtcggaat gggcttggat gagttctcta tgtcagcaac atctgtactt cgtacacgca
     2101 gcttgatgaa gaaactcgac acagctaaga tggaagagta cgcaaaccgt gcccttacag
     2161 aatgctcaac aatggaagaa gttcttgaac ttcaaaaaga atacgttaat tttgattaat
     2221 cgaaaagtcc ctgcaactca gttacaggga ctttttttga tattttaaaa agaattttca
     2281 agaaaatctt tcttatagaa agtccaacct tgaaaaagta gtggtcagaa caaaaaatac
     2341 ttaaatggtt cataaaattc ttgacaagtt ggagatttag gagtaaacta ttaaccagtt
     2401 aagtaataga gaggagtttc tgcaatttag aaatgaattg caactagaaa tatcaaatag
     2461 aaagagagtt tcgatgaaaa ttaataagaa ataccttgtt ggttctgcgg cagctttgat
     2521 tttaagtgtt tgttcttacg agttgggact gtatcaagct agaacggtta aggaaaataa
     2581 tcgtgtttcc tatatagatg gaaaacaagc gacgcaaaaa acggagaatt tgactcctga
     2641 tgaggttagc aagcgtgaag gaatcaatgc tgagcaaatc gtcatcaaga taacagacca
     2701 aggctatgtc acttcacatg gggaccacta tcattattac aatgggaagg ttccttatga
     2761 cgctatcatc agtgaagaat tgctgatgaa ggatccaaac taccagctaa aggacgagga
     2821 tattatcagt gaaatcaagg gtggttatgt tatcaaggta gatggaaaat actatgttta
     2881 ccttaaggat gcagctcatg cggataatgt ccgtacaaaa gaagaaatca atcggcaaaa
     2941 acaagaacat agtcagcatc gtgaaggagg gacttcagca aacgatggtg cggtagcctt
     3001 tgcacgttca cagggacgct acaccacaga tgatgggtat atcttcaatg catctgatat
     3061 cattgaggac acgggtgatg cttatatcgt tcctcacggc gaccattacc attacattcc
     3121 taagaatgag ttatcagcta gcgagttggc tgctgcagaa gccttcctat ctggtcggga
     3181 aaatctgtca aatttaagaa cctatcgccg acaaaatagc gataacactc caagaacaaa
     3241 ctgggtacct tctgtaagca atccaggaac tacaaatact aacacaagca acaacagcaa
     3301 cactaacagt caagcaagtc aaagtaatga cattgatagt ctcttgaaac agctctacaa
     3361 actgcctttg agtcaacgcc atgtggaatc tgatggcctt attttcgacc cagcgcaaat
     3421 cacaagtcga accgccagag gtgtagctgt ccctcatggt aaccattacc actttatccc
     3481 ttatgaacaa atgtctgaat tggaaaaacg aattgctcgt attattcccc ttcgttatcg
     3541 ttcaaaccat tgggtaccag attcaagacc agaacaacca agtccacaat cgactccgga
     3601 acctagtcca agtc
//
LOCUS       DAVKJU010000102         3315 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVKJU010000102 DAVKJU010000000
VERSION     DAVKJU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.102"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            44..>3315
                     /locus_tag="VW191_001952"
     CDS             44..>3315
                     /locus_tag="VW191_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEU9624662.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIEIKIDFQEEI
                     QENPDLVEGIVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALSKAVITDKG
                     ETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVE
                     PEKVEAPKEYTGKIEQPSAEDTKPENGASSTNGESERPKDKIKEEKQVDKKLELRNVS
                     NVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVY
                     KIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGA
                     TMDAREVELPDDQESYVKNEFYGKLIGKNNGKYYAIYNLKKPLFKTLNTATIQNLSIK
                     EANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGR
                     ITNTYDTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSA
                     TIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAIAG
                     YNFNGIKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDADKVKEKKVELVSKH
                     STKVEDFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRETIVKYGNLVEDNSD
                     LFTKKLLSVVPMKNNEVITDINKNKQEINKLLLHFEGNKSRVLNIAYKNDFSKVAEYD
                     IA"
BASE COUNT         1287 a          472 c          649 g          907 t
ORIGIN      
        1 aaggtctttg taacatatat aaattttatt tcaaggagga ataatggaaa agtattttgg
       61 tgaaaaacaa gagcgttttt catttagaaa attatcagta ggacttgtat ctgcaacgat
      121 ttcaagttta ttttttatgt ctgtattagc tagttcatct gtggatgctc aagaaactgc
      181 gggagttcac tataaatatg tggcagattc agagctatca tcagaagaaa agaagcagct
      241 tgtctatgat attccgacat acgtggagaa tgatgatgaa acttattatc ttgtttataa
      301 gttaaattct caaaatcaac tggcggaatt accaaatact ggaagcaaga atgagaggca
      361 agccctagtt gctggtgcta gcttagctgc tctgggaatt ttaatttttg ctgtttccaa
      421 gaaaaaggtt aagaataaaa cggtattaca tttagtattg gttgcgggaa taggaaatgg
      481 tgtcttagtt tcagtccatg ctttagaaaa tcatcttttg ctaaattaca atacggacta
      541 tgaattgacc tctggagaaa aattacctct tcctaaagag atttcaggtt acacttatat
      601 tggatatatc aaagagggaa aaacgacttc tgattttgaa gtaagtaatc aagaaaaatc
      661 agcagccact cctacaaaac aacaaaaggt ggattataat gttacaccaa attttgtaga
      721 ccatccatca acagtacaag ctattcagga acaaacacct gtttcttcaa ctaagccgac
      781 agaagttcaa gtagttgaaa aacctttctc tactgaatta atcaatccaa gaaaagaaga
      841 gaaacaatct tcagattctc aagaacaatt agccgaacat aagaatctag aaacgaagaa
      901 agaggagaag atttctccaa aagaaaagac tggggtaaat acattaaatc cacaggatga
      961 agttttatca ggtcaattga acaaacctga actcttatat cgtgaggaaa ctatagagat
     1021 aaaaatagat tttcaagaag aaattcaaga aaatcctgat ttagttgaag gaattgtaag
     1081 agtaaaacaa gaaggtaaat taggtaagaa agttgaaatc gtcagaatat tctctgtaaa
     1141 caaggaagaa gtttcgcgag aaattgtttc aacttcaacg actgcgccta ttccaagaat
     1201 agtcgaaaaa ggtactaaaa aaactcaagt tataaaggaa caacctgaga ctggtgtaga
     1261 acataaggac gtacagtctg gagctattgt tgaacccgca attcagcctg agttgcccga
     1321 agctgtagta agtgacaaag gcgtaccaga agttcaacca gcgttgtcca aagcagttat
     1381 aaccgacaaa ggtgagactg aggttcaacc agagtcgcca gatactgtgg taagtgataa
     1441 aggtgaacct aaacaggtag caccgcttcc agaatataca ggaccacaag ccagcgctat
     1501 tgttgaacca gagcaggtag caccactacc agaatacaca ggggtacaag ccggatcgat
     1561 agtcgagcca gagaaagtag aagcaccgaa agaatatact ggtaaaatcg aacagcctag
     1621 tgcagaagat actaaacctg aaaatggggc aagctctaca aatggggaat cggaacgtcc
     1681 taaagataaa attaaagaag aaaaacaagt cgacaagaaa ctagaactta gaaatgtttc
     1741 aaacgtggaa ttatatacgg tagaaaataa taaatatcga catattacgg ctgtagatgg
     1801 tgctttagat agttcattga agtatttcat gaaggtaaaa tcagaaaatt ttaaagatat
     1861 aatgttgcct gttactaaaa ttgaaagtac aacaaaaaac aataaagaag tatacaaaat
     1921 tgtggctcat gcagaaaatc taattcaaca cgaaaacaat gttatttcga atgactatac
     1981 atattattta ccaaaaactc agcaaagtga aactggcgtc tatacatcat ttaaaaattt
     2041 agttgatgca atgaatagtg accctaatgg aacattccat ttaggtgcaa ctatggatgc
     2101 tcgtgaagtt gaacttccgg atgatcaaga aagttatgta aaaaatgaat tttacggaaa
     2161 attaataggt aaaaataatg gtaagtatta tgctatttat aacttgaaaa aacctttatt
     2221 taaaactcta aacaccgcta ctattcagaa tctttcaata aaagaagcga atgtttcttc
     2281 taaagaagat gctgctacaa tatcgaaaga agcaaaatac aatactctta tagataatgt
     2341 tcattctgat ggtattatag ctggtgaacg tggaattggt ggattggttt caaaagtaga
     2401 taactcgaga atttctaata gtagttttac aggcagaata actaacacat atgatacaac
     2461 tgcagggtat gaaattggtg gtttagtagg taaactttct ggttcgcttg cttctataga
     2521 gaaatcaata gcatcgattg acatagcctc taatgcaaaa tcaggagatc aaattgttgg
     2581 tggtatagcc ggagttgttg agaaaagtgc gacaattaag tatagctatg ttgaaggaaa
     2641 tgttaacaat gtgcgacact ttggtaaagt tgggggagtt gcaggtaatc tttgggatag
     2701 agattcacaa gatgtaagta aatcaggtaa actatcatat gttttaagtg atgtcaatgt
     2761 tacgaatggt aatgctatag ctggctataa tttcaatgga ataaaaacga ttgaaacata
     2821 tagcaataaa aataataagg tagtaaacgt agtacaagaa gatgatgagg ttgtgactaa
     2881 agattcagat gtacaaagag gtacggtatt agatgctgat aaagtaaaag agaaaaaggt
     2941 agaattggtt tctaaacaca gcactaaagt agaagatttt gattttactt caagatacaa
     3001 tactaattac aatgaagtaa caggttacca acagtcgaga gaacaagtat ataaaaatat
     3061 tgaaaaatta cttcctttct ataacagaga aacaatagtt aagtatggta atttagtaga
     3121 ggataacagt gacttattta ctaaaaaact tctatcagtt gttcctatga agaacaatga
     3181 agtaataact gatattaata aaaataaaca agaaataaac aaattgttat tacattttga
     3241 aggtaacaaa tcacgagtat taaatattgc atataagaat gatttctcta aagttgctga
     3301 gtatgatata gctaa
//
LOCUS       DAVKJU010000103         2946 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVKJU010000103 DAVKJU010000000
VERSION     DAVKJU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.103"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VW191_001953"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VW191_001953"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEU9624663.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VW191_001954"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VW191_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEU9624664.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLSQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VW191_001955"
     CDS             2285..2818
                     /locus_tag="VW191_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEU9624665.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          944 a          540 c          529 g          933 t
ORIGIN      
        1 cttttcaaac tttgtcaact acttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gatacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tcgctctcta ccacttatcc
     1621 tttaaagcac gcactgaaaa agaagtcaga gaatatctga aaaaatacga tattgataaa
     1681 aacatcgttt ctcaagttat tgctaatctt aaagaagata agtggattaa tgatggtcag
     1741 tacgcttatg ctatcatcaa taccaatcaa ctttcaggag acaagggtcc ttatgtgctg
     1801 actcagaaac tatctcaaaa agggatttca aaatctacta tagaagagaa cttgaaagaa
     1861 tttgattttt ctgaagttgc tcaacgtgta gccaataaac tattgaaaaa atatgaggga
     1921 aaacttccag ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattctg atgctaaaat tgcctttgac gacttagata gccaagttga ccaagaaacg
     2041 actcaagaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc tcgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttaggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc tttttttggt agtataaaat aaattgtgta
     2881 aacacaaaaa ggaataaatc tattatagca gagttacaac acattactag aaagagattt
     2941 atgcaa
//
LOCUS       DAVKJU010000104         2922 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVKJU010000104 DAVKJU010000000
VERSION     DAVKJU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.104"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(105..2363)
                     /locus_tag="VW191_001956"
     CDS             complement(105..2363)
                     /locus_tag="VW191_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU9624666.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLKEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2592..2822)
                     /locus_tag="VW191_001957"
     CDS             complement(2592..2822)
                     /locus_tag="VW191_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEU9624667.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
BASE COUNT          788 a          603 c          606 g          925 t
ORIGIN      
        1 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
       61 aaagaagcag aaaaatttca ttttctactc cttttctata ggatttattt ttctttttca
      121 gaacttttaa cttcagcttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      181 actgctttga gatctttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      241 atatagtctt gaatagtccg acgaagtggg cgtgctccca tttttggatc ataacctagg
      301 tcaaccaact tttccttgac cttatcagtt acatccaaac gaatgttgtt gctagagagg
      361 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      421 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      481 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      541 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      601 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      661 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      721 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg ggcgccgacc
      781 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      841 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
      901 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
      961 cggcgaatag ccttggcaat cttatcgact gcatcatctt ggccaataac atgagacttg
     1021 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1081 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1141 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1201 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1261 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1321 gcttcatcta agaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1381 gaaagagttg cagctgcttc aatcgcagca tcggtatatt gaacgtggtg gtaatcttcg
     1441 tatttctttt gaatcccttt gagaatagtg attgtttcat ccaccgttgg ttcatcgact
     1501 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1561 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1621 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1681 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1741 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1801 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1861 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     1921 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     1981 tctccacgac gggcaatttc agttacatta ataccaaatt ctttcagcag gcctttttct
     2041 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2101 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2161 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2221 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2281 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2341 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2401 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2461 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2521 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2581 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2641 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2701 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2761 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2821 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     2881 tagaaaaaac taagatttct cgctaattct gctagtttaa ag
//
LOCUS       DAVKJU010000105         2917 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVKJU010000105 DAVKJU010000000
VERSION     DAVKJU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.105"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..881
                     /locus_tag="VW191_001958"
     CDS             <1..881
                     /locus_tag="VW191_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9624668.1"
                     /translation="YKFKEFLLQECLNKKHQIIITTHSTQLIKDFPREAVKLLVKNGE
                     KVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSENLKQNLVVRY
                     IPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLENGVVISDKIPE
                     SDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKYVSYLPFPTPE
                     FFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQEQRRAVSKIQP
                     ESSMFQCIKEKVEALF"
     gene            1261..2064
                     /locus_tag="VW191_001959"
     CDS             1261..2064
                     /locus_tag="VW191_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEU9624669.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(2180..2917)
                     /locus_tag="VW191_001960"
                     /pseudo
     CDS             complement(2180..2917)
                     /locus_tag="VW191_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          942 a          466 c          528 g          981 t
ORIGIN      
        1 tctataaatt taaagagttt ttacttcaag agtgtttaaa taaaaaacat caaattatta
       61 tcactacaca ttctacacaa cttataaaag attttcctag agaagccgtg aaacttttag
      121 tgaaaaacgg agaaaaggta gatgttattg aaaatattga ttatcaggat gcattctttg
      181 aattaggtga tgtgtatcat tctaggaaga tgatttatgt tgaagataga ctagctaaat
      241 atattctaga gtttgttatc actcattcag gtagtgagaa tcttaaacag aatttagtag
      301 tgagatatat tcctggtgga gcaaatcaaa taatttgtaa taatatttta aactcatcgt
      361 atttagattc cgataaccat tatttttggc ttgatggaga tcaaaacact aatgttagtg
      421 aatcaaataa tttaatgaac tatcttgaaa atggtgttgt tatatcagat aaaattcctg
      481 aatcagataa taaaaatctt gatgatatta taaaattgat aacgggatgt ccaattaaat
      541 ttaatgtttc aggtaataaa gggcaaaaaa ataatattga attaattgcg aaacaaagaa
      601 gctttataga ttattgggct aaatacgttt cttatcttcc tttcccaacc ccagagtttt
      661 ttttagctaa tttgtgtaac agcgtagata gggaaggtta tgatttttct aaagatggca
      721 acggaaaaga atattttaga aaaaaaactc aagtagcgtt aggaatagag aatataactt
      781 ctgaagatat tttccaggag caaagaagag ctgtttcaaa aatacagcca gaatcatcaa
      841 tgtttcagtg tattaaagaa aaagtagagg cattgttcta attccttaga ttaggtacta
      901 cataacaaat tttttaaatt caaacccgca ccaaaaacaa cattgcaact ttcttctcag
      961 gtaatgtgtt gggatttgaa cagaccaacg agttcataga ggtgtatgcc aatgagtttg
     1021 ataagtatgc gcgtcagact tattagttca tttatccaga tatatctcga taatcatatt
     1081 atttagtcaa agaacatccc atatgagggc atgaaatgct aagtttagct tgctcactat
     1141 taagatgaag tatatatatt tcatagaact tattgtgaag gatttttata attttcgcca
     1201 attccatacc catttcacca tttaccgatg acacagaaaa acaattgaaa ggatatttct
     1261 atgcaaccaa cttacaacat tgacaatcca aacttgtctt atgaagcgaa gcgtgattta
     1321 tggcggatag gttttggtct gcagaaagtt gacaatctag tgccatcagc gtatatggaa
     1381 tctttggctg agaaacagtc ccggggagaa ctgacttatg agcaggttta tgaggatgca
     1441 acggcttatc accatactat tgatgcaagt acggaagagg cagacttggt ttctctacgt
     1501 attgtagaac tattgtctcg aagaggcttt agctttagtc ctgcgacctt acttgctatt
     1561 cataaggagt tgtttcaaga tatatttgaa ccctctattc cggtggggca atttcgtcag
     1621 accaatatca caaagaatga acctgttttg aatggtgaaa gtgttgtgta ctctgattac
     1681 tccatgattc aaatgacctt ggattatgat tttaatcagg aaaaacaagt tgcatatgcg
     1741 acactaactc aggcggatgt ggttaaacaa atccagcatt ttatttcagg aatctggcag
     1801 attcatccat ttcgcgaagg caacactcgg acagtaacgg tctttttgat tcagtatctt
     1861 cgtgagtttg gttttgatat tgataatata ccatttcagc aacattccaa gtattttcgt
     1921 gatgccttgg tattagataa tgcaaagatt ttacagcgac gtcctgagtt tttaacagct
     1981 ttttttgaaa atctcttgct cggtggtcaa aatgatttgt cttcagaaaa aatgtatcta
     2041 gatttagacc ttgatttttc ataatccaaa tactgagtaa acattgaatt ttaggccaaa
     2101 atgaagtaaa tattctcaca agaaaacgta tatcatcaaa gttttaggtg tactgatccc
     2161 aaacagttag acaattaatt tatccgaagg atttagttct gtattgcaca gggataagtc
     2221 cttttagttt taccttaatt cgtttattgt tgtagtaatc aatatagtct acaatagttt
     2281 gttccaattg cttaagcgac tgaaacgact tctcataacc gtaaaacatt tccgatttca
     2341 gaatcccaaa gaaagattcc atcataccgt tgtcttggct gtttcccttg cgtgacatag
     2401 atgcttgaat tcccttaccc tcaagaaact gatgataaga atcgtgttga tattgccagc
     2461 cttggtcact atggagaatc gtattctcgt agtgcttctc tgtgaatgcc tgttccaaca
     2521 ttgtttgtac ttgttctaag ttgggtgaag ttgaaagatt ataggcgata atttcgctat
     2581 taaagccatc taaaactggt gataagtaaa gcttttgagt acttgctgga atggcaaatt
     2641 ctgtcacatc tgtgtagcac tgttccattg ttttagagcc ttcaaattgg ccttgaatga
     2701 gattctctgc cttcttgcca acgtctcctt tatgagaaga atattttcgt ttctgtcgca
     2761 ttttagcttg taaattgagt acttcatcaa gtgttgaact cttttatgat ttaccagata
     2821 accacgattt cttagttcta aataaatccg acgataacca taatttccct tgtgttcgat
     2881 aaaaatggat tgaatttcag ctttaagctc ttggtcc
//
LOCUS       DAVKJU010000106         2897 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVKJU010000106 DAVKJU010000000
VERSION     DAVKJU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.106"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            365..841
                     /locus_tag="VW191_001961"
     CDS             365..841
                     /locus_tag="VW191_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU9624670.1"
                     /translation="MIGKNIKSLRKTHDLTQHEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            841..1617
                     /locus_tag="VW191_001962"
     CDS             841..1617
                     /locus_tag="VW191_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEU9624671.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQSIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQENGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNPARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAAEVLQEYLFGKWSKVEEEMLKVGEERMRDMSKSNGKDA"
     gene            1601..2410
                     /locus_tag="VW191_001963"
     CDS             1601..2410
                     /locus_tag="VW191_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HEU9624672.1"
                     /translation="MEKMHSYIKYLTQEDPIITDEGKQIEILHLDIQDDPEIFEDWAK
                     QFRRNYCSDDELAEMIGFMNISPKEYLENFKLPSDSGIGLSTMSGDFGEILVSDYLQY
                     IEEYVVPRTRYDSKVNKNTSTQGSDVLGYKQDSSNVSNDEVVVIEVKSSASDSSSSKA
                     KNKLQEAINHSDKDFERFSTSIVASYLRLKKSNSDQANVVERFLNITDKPFNVIYGAV
                     AVYSNQSYDIDVIKTVVSKNHRDFQKLRLLVIHSNELMKFIRELYSKASEV"
     gene            2407..>2897
                     /locus_tag="VW191_001964"
     CDS             2407..>2897
                     /locus_tag="VW191_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence associated protein"
                     /protein_id="HEU9624673.1"
                     /translation="MIEFGRRSLYFSKLVRSKAKMIEFDIPLESHLPISEDAQKSFLG
                     ALAIVADTARKYFEVYINRKSFNLQLKNQLHNAAEYFDALLVSGLGNSAEYQDYIAIL
                     GTTAYYLCDYNGSSRVMINYISDDIQLLEDCMTLIKVFIDVVTDELFLNHTPIEGKYS
                     SEL"
BASE COUNT         1044 a          387 c          563 g          903 t
ORIGIN      
        1 agtaaaaaca acactttatt gaggtattgg atacgctttg ctaatagcct aataatcaca
       61 tgtggagtgt tgctacaacg aaaaaggtga taatccttga tttcaagcta ttttataagc
      121 attttgtctt tgtagataaa ggcaattttg acaataaaaa tcctaaaagg tgaatcgtta
      181 tagatgtatt tgtagatatc gtttgcgcat cgaaaaaatt aatacaagaa taaatattta
      241 tggctcttta ggtgactttt atagaagtaa agtttaggat agaaaaacag tcaataacgc
      301 accatttttg gtgcgttatg cttttttatg ctataatgga tttataaaaa taaaggagtt
      361 tgccatgatt ggaaaaaaca taaaatcctt acgtaaaaca catgacttaa cacaacacga
      421 atttgcacga attataggaa tttctcgaaa tagcttgagt cgttatgaaa atggaactag
      481 ttcagtctct acggaattaa ttgacatcat ttgtcagaag tttaatgtat cttatgtcga
      541 tattgtagga gaagataaaa tgctcaatcc tgttgaagat tatgaattga ctttgaaaat
      601 tgaaattgtg aaagaaagag gtgctaatct attatctcga ctctatcgtt atcaagatag
      661 tcagggaatt agcattgatg atgaatctaa tccttggatt ttaatgagtg atgatctatc
      721 tgacttgatt cagacaaata tctatttagt agaaaatttt gatgaaatag agagatatag
      781 cggctatttg gatggaattg aacgtatgtt agagatatct gaaaagcgga tggtagctta
      841 atggaaatcc aagattatac tgatagtgaa ttcaaacatg ctttagcaag gaatcttcgt
      901 tcactgacaa gaggaaaaaa gtccagtaaa caatctatag cgattttgct tggaggtcaa
      961 agtggtgccg gtaagactac aattcatcgt attaaacaga aagaatttca aggaaatatt
     1021 gttatcatag atggtgatag ttttcgttct cagcatccac actatttaga actgcagcaa
     1081 gaaaatggca aagatagcgt tgaatacacc aaagattttg cagggaaaat ggtagagtct
     1141 ttagtaaccg aattgagtca tttgggatac aatcttttga tagagggaac tttacgaact
     1201 attgatgttc caaagaaaac agcacaacta ttaaaaaata aggggtatga ggtacaatta
     1261 gccttaattg caaccaagcc cgaattgtcc tatctgagca cccttatccg atacgaagaa
     1321 ctgtatgcta tcaatccaaa tccagcacgc gcaactccaa aagaacatca tgatttcatt
     1381 gtaaatcatc tagttgataa tacacgacaa ttggaagaac tagctatttt tgaaagaatt
     1441 caaatttacc aacgagatag aagttgtgta tatgattcaa aagaaaatac aacttcagca
     1501 gcagaagtgc tacaagaata cctcttcgga aaatggagca aggtagagga agagatgttg
     1561 aaggtgggag aagaacggat gagggatatg agtaaaagta atggaaaaga tgcatagtta
     1621 tataaagtat ctaactcaag aagatcctat tattactgat gaaggtaagc agattgagat
     1681 tcttcactta gatattcaag atgacccaga aatttttgaa gattgggcaa aacaattcag
     1741 aagaaattat tgttctgatg atgaacttgc tgaaatgata ggttttatga atatctcccc
     1801 taaagagtat cttgaaaatt ttaaattacc ctcggatagt ggtattggcc tttcaacgat
     1861 gtcaggagat tttggagaaa tattagtatc tgattatctt cagtatattg aagaatatgt
     1921 ggttcctagg actcgttatg atagtaaagt caataaaaat acttcaactc aaggaagtga
     1981 tgttctagga tataagcaag attcttcgaa tgtgtcgaat gatgaggttg tagttattga
     2041 agtaaaatca tctgcatcag attctagtag ctctaaagcg aaaaataagc ttcaagaagc
     2101 tattaatcat tctgataaag attttgaacg attttcaacc tcaattgtag ccagctattt
     2161 gaggttaaag aaatctaata gtgatcaagc aaatgtagta gaaagatttt taaacataac
     2221 ggataaaccg tttaatgtaa tctatggagc tgttgcagtc tactccaatc aatcatatga
     2281 tattgatgtt atcaagacag tagtttcaaa aaatcacaga gattttcaga aattacgact
     2341 attggtcatt cattctaatg aattaatgaa atttattaga gaactctatt cgaaagcgag
     2401 tgaggtatga tagaatttgg gagaaggtca ttatattttt caaaattagt gcgttcaaag
     2461 gcaaagatga ttgaatttga tattccttta gaaagtcatt taccaatttc agaggatgct
     2521 caaaaatcgt ttcttggggc actagcaatt gttgcagata ctgctaggaa gtattttgaa
     2581 gtttatatca atcgtaagag ttttaattta caattaaaaa atcaattaca caatgctgct
     2641 gaatattttg atgcattgct ggtgtcagga ttaggaaatt cagctgaata tcaagactat
     2701 attgcaatat tgggtaccac agcttattat ctatgcgatt ataatggtag ttcgagagtt
     2761 atgattaact atatatcgga tgatatccag ttattagaag actgtatgac tcttataaaa
     2821 gtatttattg acgttgtaac ggatgaactc tttttgaatc atacgccaat agaaggtaaa
     2881 tactctagtg agttaaa
//
LOCUS       DAVKJU010000107         2893 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVKJU010000107 DAVKJU010000000
VERSION     DAVKJU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.107"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(85..1026)
                     /locus_tag="VW191_001965"
     CDS             complement(85..1026)
                     /locus_tag="VW191_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEU9624674.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VW191_001966"
     CDS             complement(1004..2767)
                     /gene="aspS"
                     /locus_tag="VW191_001966"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU9624675.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
BASE COUNT          844 a          677 c          583 g          789 t
ORIGIN      
        1 agatagaact gacgaagtca gtaacataca tacggtaagg cgacgctgac gtggtttgaa
       61 gagattttcg aagagtatta atcactaatt atctatctca acaaatcttc ctagaatatg
      121 aacattttcc gagacagaga caaaggagct tggatccact tgtgtcataa tctgtttaaa
      181 ttcattaaac tctgcacgtg taatgacagt gattaaaact gcctttctct cgtgattata
      241 ggttccttct gcatcgtgga tcatggttgc tccgcggtgc aattttttat ggattttttc
      301 aattaccttc tctggatgat ttgtcacaat catggcctgc atacgctttt gcttagtaaa
      361 gactgcgtct gtcacacggc tagagacaaa gatggtaatc atagaataaa gagcgtattt
      421 ccaaccaaag gtcaaacctg ctatcagcat gatagttcca tttaccaaga aagaaatact
      481 accgacattc ttacccgttt tcttgcgaat agtcaggctg acgatatccg tcccaccact
      541 ggaaatattg tttcgaagag caaaaccaat ccccaaaccc ataacaacac ccccaaaaag
      601 ggaattgata atgggatcct ccgtcaaggt tgccacaggg acaaactgga taaagaagga
      661 actcatagat accgtgataa aggtaaagac ggtgaactta tggccaatct gataccaagc
      721 taagaccatc aaagggaagt taatggcgta gaagcttagc gaaatcggaa tatgaaaacc
      781 aaaccagtga ttactcaagg cagagataat ctgtgccaga cctgttgcac cactcgaata
      841 cacatgccct ggttggaaaa agaaattaac tgctactgct gataaaaaac catagaccag
      901 agaggccgaa atcttctcat catacttctc tcgagagata ctttgtaaga cacgtaaaat
      961 ttttatctga taagcaaagc ggcgcagata atagcgccac cgcttaattc gttttgtttg
     1021 tttcatcttc ttctacttgt aagctgagtt cctctagttg tttgagagcg actgttgatg
     1081 gagcttgtgt cattgggtca gttgccttgt tgttcttagg aaaggcaatg acttcacgga
     1141 tattttcttc tccagcaagc aacatgacaa aacggtcaag cccgatagcc aaaccaccgt
     1201 gtggtgggaa accatagtcc atggcttcaa gaaggaaacc aaactggtca ttggcttctt
     1261 cagttgagaa accaagagcc ttgaacatgc gttcttgaag gtctttttgg ttgatacgaa
     1321 ggctaccacc accaagctca taaccgttca agacgatatc gtaagcaatg gcacgaacct
     1381 tagccaaatc accttctaat tcgtgagcag tctcttcctg tggaagtgtg aatggatggt
     1441 gggcgctcat gtagcggcct tcttcttcag accattcaaa catcggccag tcaaccaccc
     1501 aaaggaagtt gaacttatca ttatcaatca agccaagctc tttagcaata cgtccacgaa
     1561 gggcacccag tgttgcatta gccacttcaa gcgtatccgc cacaaagaga accaagtcct
     1621 tatcttcaag agcaagcgct gttgtcaatt cttcttggat accagtcaag aacttggcaa
     1681 ctggtccgtt taattctcca tcaaccacct tgacccaagc aagacctttg gcaccatact
     1741 gtttggctac ttccgtcatc ttgtcgatgt ctttacgtga atagttgtcc gcagctcctt
     1801 tgaccacaat cgcttttaca gcaggtgctt ctgaaaagac tttaaagtct acacctttga
     1861 ccacttctgt caagtcctga agcaacatgt caaaacgagt atctggcttg tcagaaccgt
     1921 aaagagccat agcatcatcg tatttcatac gagggaacgg tagcgttact tcgatgcctt
     1981 ttgtttcctt catcacgcgc gcgatcaagc cttctgtaat atcttggatt tcttgctcag
     2041 taaggaagga cgtttccaag tcgacctgag taaattcagg ctggcggtct ccacgcaagt
     2101 cttcgtcacg gaaacattta acgatttggt agtaacggtc aaaaccagca ttcatcaaga
     2161 gctgtttcgt gatttgtgga ctttgaggaa gagcgtaaaa atgcccctta ttaacacgag
     2221 acggcactaa ataatcacgc gccccttcag gcgttgactt agaaaggaat ggtgtctcca
     2281 cgtcgataaa ctccaactca tccaagtagt tgcggataga gtgggtcacc ttggcacgaa
     2341 gtttaagatt ttccaacatt tctggacgac gaaggtcaag gtaacggtaa cgcaaacgtg
     2401 tatcgtcatt tgcctcaatg ccatccttaa tctcaaatgg tgttgtctta gctgtgttaa
     2461 gcacaataag agctgtcacg tttaactcaa ccgcaccagt tggcaactta tcattggctt
     2521 gctcacgcgc agcgacctga ccagtcacct caataacaaa ttcgctacga aggctttcag
     2581 ctgttgccat aacctctgca gatacttttt cagggttgat aaccaactgc atgattcctt
     2641 cacggtcacg aagatcgata aagatcaaac caccaaggtc acgacgacgg ccaacccatc
     2701 ctttcaaggt tatttcttgt ccgatgtgtt cctcacgaac acgaccagca tacatactac
     2761 gtttcattat ttctctcctc ttttattctg ttactatttt accataaaag cgcaggctct
     2821 tcatgaaaat catcagaaaa gtttgccagt ctttaaaagt caggtgaaag ccctaaaaat
     2881 tagcgctaat act
//
LOCUS       DAVKJU010000108         2770 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVKJU010000108 DAVKJU010000000
VERSION     DAVKJU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.108"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..870
                     /locus_tag="VW191_001967"
     CDS             <1..870
                     /locus_tag="VW191_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9624676.1"
                     /translation="IKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKF
                     DIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQ
                     ILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLAN
                     CTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNE
                     KKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKN
                     LIEKTKMAEEFIY"
     gene            881..1465
                     /locus_tag="VW191_001968"
     CDS             881..1465
                     /locus_tag="VW191_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEU9624677.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLKKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1465..2160
                     /locus_tag="VW191_001969"
     CDS             1465..2160
                     /locus_tag="VW191_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEU9624678.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2148..>2770
                     /locus_tag="VW191_001970"
     CDS             2148..>2770
                     /locus_tag="VW191_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624679.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDR"
BASE COUNT          997 a          359 c          533 g          881 t
ORIGIN      
        1 ataaaagact ctttagatgc atctaaagag ggcttgatgg aaatatttta tttatcgccc
       61 aaaatagaaa gattaaaaga aattcaaaat caagatttac aagaaggcga tgactatagc
      121 ttaaaaaaat ttgatattga tctaaaagat gttgagtttg cctacaataa agacgcaaaa
      181 gttttaaatg gtgtaagttt taaagctaag cagggagagg tcactgcttt ggtaggtgca
      241 agtggctgcg gtaaaacaac tatcttgaaa cttatatcaa gactttatga ttatgacaag
      301 ggacaaatct taatcgatgg caaagatata aaagaaatat caacagaatc tctttttgat
      361 aaggtctcta tagttttcca agatgtggtt ctctttaatc aaagcattat ggaaaatatt
      421 agaatcggaa agcaagatgc aagtgacgaa gaggttaaaa gagcagcaaa acttgcaaat
      481 tgcacagatt ttatagaaaa aatggataaa ggtttcgata cagttattgg tgagaacgga
      541 gctgagctat caggaggaga aagacaaaga ttatcaatag ccagagcctt cttaaaagat
      601 gcgccgatat tgatcttaga tgagataaca gcaagccttg atgttaacaa cgagaaaaag
      661 attcaagagt ctttaaataa tttagttaaa gataaaactg ttgtaatcat ttcacataga
      721 atgaaatcca tagaaaatgc agacaagata gtagttcttc aaaacggaag agtagaaagc
      781 gaaggtaagc atgaagagct tttacaaaaa tcaaaaattt acaaaaattt aatagaaaag
      841 acaaaaatgg cagaagaatt tatttattag gaggactaca atggataata aaaaattaaa
      901 agtaaaagat ttagtaagta tcggtgtttt tggcgtaatt tattttgcct tcatgtttgg
      961 agttggtatg atgggcttga ttccaatatt gttcttaata tacccgacag tattagccat
     1021 agttgcagga actgttgtta tgttatttat ggctaaggtt caaaagccat gggcactatt
     1081 tatatttggt atgatatcac cacttgtgat gtttgcagct ggtcatacct acgtagttgt
     1141 ggttttatca cttatagtaa tgataatagc agaattaatt agaaagattg gtaattataa
     1201 ttcatttaaa tacaatatgc tttcttatgc aatcttcagc acatggatat gtagctcttt
     1261 aatgcaaatg cttttagcaa aagaaaaata tatggagtgg tctttgatga cgatgggaaa
     1321 agattatgtt gatgtattaa aaaagttaat aacttatcct cacatggctt tagtagcctt
     1381 aggtgctttc ttaggaggaa ttcttggagc atatataggc aaggctctat tgaaaaaaca
     1441 ctttgaaaaa gcaggcattg tataatgcct actttttctt caaatgctcc ctttaatccc
     1501 aatccgatct ctaagatatt agtcgtattt atcacaggct taactgttat gcatagcata
     1561 aacatccgtt ttgagctggc aattgtttgt cttattggta ttttgcttta tttgaatgga
     1621 tacaaaaaaa cacttttcaa atggattata ttgtgtggga tactttactc cttgcctaat
     1681 tttatggtgt tatctgaatt aaaccctata gttaagatgt ttttgagtat acctattgtt
     1741 attagaatgt ttattttacc atttatggca gcaagcttta tgataaagac ctcggatgta
     1801 ggcgcaataa tttcatcgat ggataagctt aagatttcaa agaatgtatc catacctatt
     1861 gcggttatgt ttagattctt cccatctttt aaggaggaga agaaaaacat caaaatggct
     1921 atgagagtaa gagggataag ttttaaaaac ccagtcaaat atcttgaata tgtttctatg
     1981 ccactactca ttatatcatc taatatatca gatgacattg caaaagcggc agaaacaaag
     2041 gcaatagaaa atccaattgc caagaccaga tacattcgcg taaagataca gctaattgat
     2101 tttgtttatg ttttagcggt tgctggactt attgtgggag gcttaatatg gttgaaataa
     2161 aaaatttaag tcttgattat ggtgaagagc atatattaga tgatatatca ctatccatag
     2221 ccgagggaga gtgcgtgcta tttacaggaa aaagtggaaa tggtaagtca tctttaataa
     2281 attcaatcaa tggactagct gtaaggtatg ataacgcaaa gacaaagggc gaaataatta
     2341 ttgatggtaa gaatataaaa aatttggaac tttatcaaat ctcaatgctt gtttcaactg
     2401 tttttcaaaa tcctaagaca tattttttta atgtcaatac gacattagaa ttattatttt
     2461 atttggaaaa tatcggtctt gcaagagaag agatggacag gcgtttgaag gatatgctta
     2521 agatattccc gataaaaaat cttttgaaca gaaatatatt taatctatcc ggcggtgaaa
     2581 aacaaattct ttgcattgca gcttcttata tagcaggtac aaagattata gttatggatg
     2641 agccttcatc gaatttagat attaaaagca taagtgtttt ggcaaagatg ctaaagatat
     2701 taaaagagaa aggcataagc ataattgttg cagagcatag aatttattat ttgatggaca
     2761 tagttgaccg
//
LOCUS       DAVKJU010000109         2744 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVKJU010000109 DAVKJU010000000
VERSION     DAVKJU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.109"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            93..1007
                     /locus_tag="VW191_001971"
     CDS             93..1007
                     /locus_tag="VW191_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin formation protein"
                     /protein_id="HEU9624680.1"
                     /translation="MYRGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKT
                     TFGLTGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLP
                     LINTLVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKN
                     IYKVTRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTF
                     CHGSMAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGV
                     TGIAYTFLRYIALNQNDKKIVIPSLLEI"
     gene            1357..2061
                     /locus_tag="VW191_001972"
     CDS             1357..2061
                     /locus_tag="VW191_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEU9624681.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            2042..>2744
                     /locus_tag="VW191_001973"
     CDS             2042..>2744
                     /locus_tag="VW191_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEU9624682.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDS"
BASE COUNT          970 a          329 c          480 g          965 t
ORIGIN      
        1 ttattaataa tatttatgac gaatatccaa tttggagtat acctgttaat tttaaaggtt
       61 ctaaatggtg tcttgaacaa acgggtaatg atatgtatag gggtagagct ggaatattac
      121 tgtttatgta ctatgtaaaa ttattgaaag ataaatcatc atatactcaa ctgtacaata
      181 aaatgttatc tgcaattccg ccatctgttt ctttatctaa gaccacattt ggtttaactg
      241 gggatatagg atatttcatt gttctatcca ttatagagga ttacgataca aatactctcg
      301 catgcttaaa ttatataaag agtgttttaa ctgagcttga aaagactgat tttgcaagtg
      361 tttcaaacaa gtctgactat attaatggtc ttctaccttt aattaataca ctggtaagat
      421 attataggag aggaatagag aaggaacgtg ttctaaggct agtattaagt ttaggtgata
      481 cgctatataa tgatgtaagc gagagagata atagtaattt tggatatagt tttggccatg
      541 gactatcagg tgtaattttt acattaaaaa atatttataa agtgacaaga cttaaaaaat
      601 ataaagaatt aattttacaa ctagttccta gggagaaaat caaaattcat tctttgaagt
      661 ggtgtagtgg agaatttggg acattagtta atcatcccca aagtgttcca gagggaattg
      721 tagctagttt agataatgat acattttgcc atggaagtat ggccataata aatttttaca
      781 tcgactacat gaaagaaatg gatactagta tggaattgga gtctttattg tgtagtgttg
      841 taatgaataa atcaaaagaa ggagagtatc gtatcgtcca aacaagcaat ttcccagatt
      901 tttctttgta tacaggtgtg actggtattg cttatacttt tttaaggtat atcgcactga
      961 atcagaatga caaaaagata gttatcccct cattacttga aatctagctt aatttatagt
     1021 tttgtttcta aaatagaggt gtccactcat taagcaatat aaaaatacca agattgacta
     1081 gtttaatatg taattctata aaatattttt aatagtgtag cttttcgttg agataataat
     1141 ataataaaga aaagttctat tctaattgct aaacggcttg aggtatatgc tactgttata
     1201 gaaatgattt taaactaaaa tcaaaattac tccaaaaata gtattgattt tggtttaata
     1261 caaaacatat gtaataatgt gagtcaacaa aagtctgttt ttgtaccata ctatcttgaa
     1321 aaatctacta aattcaaatt gagaggatga attaaaatga ataaaatatt tatatatgca
     1381 ggagtgcgaa atcataactc taagacttta gagtatacta agagactttc aagtatcatt
     1441 tctagtagaa ataatgttga tatatctttc aggacacctt tcaattcaga gctagaaata
     1501 tctaattctg attcagaaga gttgtttaag aaaggaattg ataggcaaag taatgctgat
     1561 gatggaggtg ttataaaaaa agagttatta gagtctgata ttataattat ctcttctcct
     1621 gtatatttac aaaacgtatc tgtggacaca aaaaacttta ttgaaaggat tggggggtgg
     1681 agccatcttt ttcgattagc tggaaagttt gttgtaacat tagatgttgc tgagagtaac
     1741 ggctctgata atgtgtcaga atatcttaga gatatatttt catatatggg aggacagata
     1801 ttacaccaag tttcaattac caattcactc aaagatattg cagaggctca gttgatggag
     1861 gcaacttata aaattgaaga tgtacttgaa gggaaaatta agtacaaaac aacggattat
     1921 caagaaagag cttaccagac attaaaatta attttagaaa attatgattc cgaacatttt
     1981 gagaagatgt attgggagaa aaaacgcctt tttgaagcta atagtttaga ggaatggtat
     2041 tatgttgaaa atattaaata actctgtcat atatttatgt ttgattccgt tattgttcct
     2101 attattgata atttttccaa atgattcatt aatctattat tttcgattga ttttaatttc
     2161 gttaatatca attacattac atgaactagg tcatttttta gtgggaagat gtttatctta
     2221 taaactagag atgcttgcta ctcccttttt cttctatttc agaaaaaaaa tatatttcaa
     2281 atttccagtg ttattagcat tcggttattg tcaaatgagt aatagaaaca ttactaatga
     2341 aaaaaatagt gataggaatt tggtgttcta cttctttggg ggaggtggag ctaacctaat
     2401 tgtcgcaata ttagcacttt tgggatttgt gccgtacgca tcagaatttt ttattttgaa
     2461 tataattctt tttctagtta cggtatgttt accaattgat ggaacagatg gcaatgctat
     2521 tagagagata gttttgtatt caaaagattc aaaaacatat cagagattct ttgcgaacag
     2581 tctctataat aatccttata taacaatcga cgattttgca aaattaacct ctaaagaaaa
     2641 atcttttttt tcaaaatttg aaaaatgttt gataaacctt tactttgaag tgaaaggtga
     2701 agggtcagca tttacaattg ctaatcatga agtttttgat tcaa
//
LOCUS       DAVKJU010000110         2588 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVKJU010000110 DAVKJU010000000
VERSION     DAVKJU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.110"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            83..1837
                     /locus_tag="VW191_001974"
     CDS             83..1837
                     /locus_tag="VW191_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624683.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            1821..>2588
                     /locus_tag="VW191_001975"
     CDS             1821..>2588
                     /locus_tag="VW191_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU9624684.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAV"
BASE COUNT          926 a          345 c          474 g          843 t
ORIGIN      
        1 gtataataga aaagcaatga tagatattat caattcagcg aatttatatc taaaagggat
       61 attaaagaaa ggagatatgc ttatgaagat ttacaaaaaa ctatttgctt atgtccaaga
      121 taagaaatat cttggggttt tggccataat tttttctgct atatctgctg cacttacagt
      181 atatggatat tatttaatct acaaatttct agataagtta ataattaatt caaacttatc
      241 cggtgcagag agtatagcat taaaatctgt tattacacta acaagtggag cgatatttta
      301 ttttgtctca ggaatgtttt cacatatctt gggatttagg cttgaaacaa atttaagaaa
      361 aaggggaatc gatggtctgg aaaaagcaag ttttaggttc tttgacttaa atccatctgg
      421 tcaaataaga aagatcatag atgataatgc agcacaaact catcaggtgg tagctcacat
      481 gattccagat agctctcagg caatagtcac acctatactt gtccttgtac taggctttat
      541 agtaagtata agggttggta taactttgct tgctcttact ataattggcg gcttaatttt
      601 aggggcaatg atgggcgagc aagaatttat gaagatatac caagaatccc tatctaaact
      661 aagtgctgaa actgttgagt acgtgagagg aatgcaagtt gtaaaaatat ttaaagcaaa
      721 tgtagagtca tttaaaagct tttacaaggc tataaaagat tactcaaagt atgcttatga
      781 ttattcccta tcttgtaaaa ggccttatgt tttgtatcaa tggttatttt ttggactgat
      841 tgcaatttta attattccta tagtttattt tatgactagc ttagctagcg caaaggtgat
      901 tttacttgag cttatcatga ttttattttt atcaggagtt ctctttgttt cattcatgag
      961 aatgatgtgg tactccatgt atatttctca aggaaattat gcagtagata ctttagaggc
     1021 gctttacgaa gatatgcaaa aagacaaatt agtgcatggt aatgtcaata attttaaaaa
     1081 ctataatata gaatttgaga atgttagctt tgcttataat gataaagctg tcattgaaaa
     1141 tttatccttt aatttagaag aaggaaagtc ctacgcactt gtcggttcat ctggatcagg
     1201 caaatcaaca gtagcaaaac ttatatcagg tttttacaat gttaataaag gaagcataaa
     1261 gataggcggg atagcaataa gtgaatattc tgacgaagcc ttaattaaag ccatttcctt
     1321 tgtttttcaa gattcaaaat tatttaagaa gagcatttat gataatgtag ctttagctaa
     1381 taaagatgcg acgaaagatg acgttatgag agccttaaaa ttagcaggat gcgatttaat
     1441 attagacaaa ttcccagaaa gagaaaatac aatcataggc tcaaaaggtg tttatttatc
     1501 cggtggagaa aaacaaagaa ttgcaattgc tagagcaatt ttaaaggatt ccaaaattat
     1561 tattatggat gaagcatcag catctattga cccagataac gagtttgaat tgcaaaaagc
     1621 ttttaaaaat cttatgaagg ataaaacagt tatcatgatt gcacacaggc tatctacaat
     1681 taaagacctt gatgaaatta ttgtcatgga tagtggaaaa attatagaaa gagggtctga
     1741 caaagaatta atgtcaaaag atacaaggta taagagcctg caagagatgt ttaacagtgc
     1801 gaatgaatgg agggtttcaa atgaaagagt tttataaaaa aagatttgct cttacagatg
     1861 gaggagcaag aaatttaagt aaagcaacac tggcttcatt tttcgtttat tgtataaaca
     1921 tgcttcctgc catattactt atgatttttg ctcaggaagt tttggaaaat atgggcaaaa
     1981 gcaatggctt ttatatagta ttctcagttt tgactttgat agcaatgtat attttgcttt
     2041 ctatcgaata cgataaatta tataacacaa cctatcaaga aagtgcagat ttaagaataa
     2101 ggacagcgga gaatttatca aaattacctc tatcttactt ttctaaacat gacatttccg
     2161 acatttcaca aacaatcatg gctgatattg aaggcataga gcatgcaatg agccactcaa
     2221 taccaaaggt gggcggcatg gtactgtttt tcccattaat atctgtaatg atgctagcgg
     2281 gcaatgtcaa gatgggttta gctgtaatta ttccatctat tttaagcttt atatttatac
     2341 ctttatctaa aaaatatcag gttaatggac agaatagata ttatgatgtc ttaagaaaaa
     2401 actcagaaag ctttcaagaa aatatcgaaa tgcaaatgga gattaaagca tataatttat
     2461 cgaaggatat taaagatgac ttatataaaa aaatggaaga tagtgagaga gtacacttaa
     2521 aggcagaagt aactacaatt ttaactttgt ctatatcttc aatatttagc tttatatctc
     2581 ttgctgtt
//
LOCUS       DAVKJU010000111         2537 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVKJU010000111 DAVKJU010000000
VERSION     DAVKJU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.111"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     misc_feature    67..202
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            241..741
                     /locus_tag="VW191_001976"
     CDS             241..741
                     /locus_tag="VW191_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU9624685.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEALASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            817..1185
                     /gene="rplL"
                     /locus_tag="VW191_001977"
     CDS             817..1185
                     /gene="rplL"
                     /locus_tag="VW191_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU9624686.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            1432..>2537
                     /locus_tag="VW191_001978"
     CDS             1432..>2537
                     /locus_tag="VW191_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9624687.1"
                     /translation="MSLRNKIEQHIKELEGGKFQKLGDAYLSRKYNFNIVSLGSQEGT
                     DKTTKGIPDSYAVENGKYVYIMYGTHKSVISKLEGDIQSVKKKILEENIAEDKVGRLI
                     CCHTSSNITIKQKEDLEKMAEPYHLELIGINEIANDLTKIDFQYLAKEYLSISESTEQ
                     VWSINDFIRIHDESKTNAPISNDYIGDVSEIINTIKSSEKRIFLISAKPGTGKTRLAI
                     EICSLLDRNKYNIICVKSNNQDIYQDVKRNLNLHKENIVFIDDVNTTQNYISTLGLLN
                     TTSNIRFILTVRDYAKKDVINNIKVYGYNNIEPELIKDDNFKELLNQFSRNDFTNQEI
                     EHIKTISKSNPRIAVIAAKLSSSQDLTNFNDEID"
BASE COUNT          904 a          381 c          482 g          770 t
ORIGIN      
        1 tacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt ctttttttga ctatgctata
       61 cttatatacg gtttaaaaaa actgcctaag acagtagggg agctcgactc ataaatatcc
      121 taccgaggac aaaacgtatc atgtaaaaag aagcgtattg tactttcgtg tctaggtttg
      181 ggcgcgtttt tctttttgaa aaattcccca agcaaaataa ttacggaggt gaacacacta
      241 atgagtgaag caattattgc taaaaaagcg gaactagttg acgtagtagc tgaaaaaatg
      301 aaagctgctg catctatcgt cgttgtagac gctcgtggtt tgacagttga gcaagataca
      361 gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata aagttattaa aaactcaatc
      421 ttgcgtcgtg cagctgaaaa agctggtctt gaagctcttg catctgtatt tgttggacca
      481 tctgcagtag cattttctaa tgaagatgtt atcgcaccag cgaaaatctt gaacgacttt
      541 tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa tcgaaggcgc tgtcgcatct
      601 aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg aaggacttct ttctatgctc
      661 ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg cagtcaaagc ggttgcagaa
      721 agcaaagaag acgcggctta atcttaagct acacagcgta gcctagctac gaaaaaaact
      781 attataaaat ttaaaactta tttggaggaa ataacaatgg cattgaacat tgaaaacatt
      841 attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg accttgtaaa agctatcgaa
      901 gaagaatttg gtgtaactgc agctgctcct gtagctgttg ctgcagctga tgcagctgat
      961 gctggtgctg ctaaagattc attcgacgtt gaattgacat ctgcaggcga caaaaaagtt
     1021 ggcgttatca aagttgtacg tgaaatcact ggtcttggtc ttaaagaagc taaagaactt
     1081 gttgacggtg caccagcact tgttaaagaa ggcgttgcaa ctgcagaagc tgaagaaatc
     1141 aaagctaaat tggaagaagc tggagcttca gttactctta aataatagag caactacatt
     1201 agtagcttaa aaacatgatt aaaccgctat tcttaggagt agcggttttt ctttttgttt
     1261 aaaaaggggc aaacttttta tatagttgtt taggaaataa ataatttttg tataattggt
     1321 ttactactct aaattaacgt agtacaagag tttgttaatt aaactatacc ttatggtttt
     1381 gactagtgtg atataattat gttgaaatac ttatctggaa aggtttttat aatgagttta
     1441 agaaataaaa ttgagcaaca tattaaagag ctagagggag ggaagtttca aaaactaggt
     1501 gacgcgtacc taagtagaaa atacaatttt aatattgttt cattaggctc acaggaagga
     1561 acagataaaa caactaaagg gatcccagat tcttacgctg tcgaaaatgg taaatatgtt
     1621 tatattatgt atggcaccca taagtctgta atatctaaat tagaaggaga cattcagtca
     1681 gttaagaaaa aaattttaga ggaaaatatt gctgaagata aagttgggag acttatttgt
     1741 tgtcatactt cttcaaatat aacaattaaa caaaaagaag atctagaaaa aatggctgag
     1801 ccttatcatc tagaattgat tggaattaat gaaattgcta atgatcttac aaagatagat
     1861 tttcaatatt tagcaaaaga gtatctatca atcagtgaat ctacagagca agtttggtct
     1921 atcaatgact tcataagaat tcatgatgag agtaaaacta atgctcctat atcaaatgac
     1981 tatattggag atgtaagtga aattataaat accattaaat caagtgaaaa acgaatcttc
     2041 ttaatatctg ctaagcctgg aacagggaaa actagattag caattgaaat ttgctctttg
     2101 ctggatcgaa ataaatataa cattatttgt gtcaagagca ataatcagga tatttaccaa
     2161 gatgtaaaac gcaatctaaa tttacacaaa gaaaatattg tttttattga tgatgttaat
     2221 actacacaaa attatatttc tacacttggg ctactgaaca cgactagtaa tattcggttt
     2281 attcttacgg tacgtgatta tgctaaaaaa gatgtaatca ataatattaa agtttatggg
     2341 tacaataata ttgagccaga acttataaaa gatgataatt ttaaggaatt actcaatcaa
     2401 ttttctcgaa acgattttac caatcaagaa attgaacata taaaaacgat atctaaaagc
     2461 aatccgcgta ttgcagtcat tgcagcaaag ttatctagct ctcaggattt gactaatttt
     2521 aatgatgaga ttgatat
//
LOCUS       DAVKJU010000112         2484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVKJU010000112 DAVKJU010000000
VERSION     DAVKJU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.112"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            46..522
                     /locus_tag="VW191_001979"
     CDS             46..522
                     /locus_tag="VW191_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU9624688.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAETTA"
     gene            560..865
                     /locus_tag="VW191_001980"
     CDS             560..865
                     /locus_tag="VW191_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU9624689.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            913..2388
                     /locus_tag="VW191_001981"
     CDS             913..2388
                     /locus_tag="VW191_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEU9624690.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          739 a          479 c          494 g          772 t
ORIGIN      
        1 gtgttatact tatggcataa agttaatgga aagaagggaa aacttatggt attagattat
       61 ttctttgaca aaaaccttgt gttttgctta gaagcggata atcaagaaca actctttgat
      121 caggttgcaa ctttattgga agaacgagaa attgtcactc caacttatcg tgaagccttg
      181 atcacgcgtg aaaagtcatt tccaactggt ttagatatgg aatttctagg aaaggacttg
      241 ccaaatgtag cgattcctca tacagatatt gttcataatc tagctgagaa agtggtggtt
      301 gttcgattag aaaaaccagt aacttttcac aatatgatag ctcctgataa ggaagtagaa
      361 gtatccttgc tcttctttat catcaataac tcaagttcaa gtcaaacaaa tattctggct
      421 cagttgatgg actttttcac aggaaatgga catcttgaag acctatcaaa aatttccgaa
      481 ccagaaaaac tttatgctta cattgctgaa acaaccgctt aatcttgtct attaaaaaat
      541 aaaatcggag gaaatctaaa tgattaaaat tcttgctgcc tgcggtgcag gtgttaactc
      601 aagtcaccaa attaaaagtg ctctagaaga agaactttca aaccgtggtt atgatgttca
      661 ctgtgatgca gtcatggtga aagatgtaaa cgaagacctt atgaaaggtt acgatatctt
      721 tacaccaatc gctgcaacag accttggttt tgaaccaggt atcccagtta tcgaagctgg
      781 accaatctta ttccgtatcc cagcaatgag cgctccagta tttgacaata ttgaagcagc
      841 tattaaagaa catggattaa gctaattcta atttgttaac attcatataa cataaaaaag
      901 ggaggaactg ttatggatgc aatctttgac ctaatcggaa aggttttcaa tcccatctta
      961 gaaatgggtg gacctgtcat catgttgatc attttgacag tattggcttt actttttgga
     1021 gtgaaattct ccaaagcgct tgaaggtggt atcaaacttg ccatcgctct tacaggtatc
     1081 ggtgctatca tcggtatgct aaatggtgct ttctcagcat cacttgcaaa attcgttgaa
     1141 aacactggta tccaattgag tattacagac gttggttggg caccactcgc aacaatcact
     1201 tggggttctg cttggacact atacttcttg ctcatcatgt tgattgtcaa cgtagtgatg
     1261 cttgctatga agaaaaccga tacacttgat gtcgatatct ttgatatctg gcacttgtct
     1321 atcacaggtc tcttgattaa atggtatgct gataacaatg gtgtgagtca aggggtttca
     1381 ctctttattg ctacagcagc tatcgtcctt gtcggtgtgt tgaaaattat caactctgac
     1441 ttgatgaaac ctacatttga tgaccttctt aacgccccaa gttcatcacc aatgacatca
     1501 actcacatga actacatgat gaacccagtt atcatggttt tggataagat ttttgaaaaa
     1561 ttcttcccag gccttgataa atatgacttt gatgctgcta aattgaacaa gaaaatcggt
     1621 ttctggggat ctaaattctt catcggtttc atccttggta tcgttatcgg tattatggga
     1681 actccacatc caattgcagg tgttgcagat gcagataaat ggcgtcttgt tatcaaagga
     1741 tggttgtctc ttggtttgac tgccggtgta tctttggaac tcttctcact tatcggttca
     1801 tggttcatcg cagccgtaga accactatca caaggtatta caaacgttgc tactaaacgt
     1861 cttcaaggac gtaaattcaa tatcggtctt gactggccat tcatcgctgg tcgtgctgaa
     1921 atctgggctt gtgccaacgt acttgcacca atcatgttga ttgaagcagt gcttctttca
     1981 aaagttggaa atggtatctt gccacttgca ggtatcatcg ctatgggtgt tactccagct
     2041 ctcttggttg taactcgtgg taaattgctc cgtatgatta tcttcggaac actcttgttg
     2101 ccactcttcc ttctttcagg tacacttatt gcaccatttg caacagaact tgctaaaggt
     2161 gtaggtgcct tcccagaagg tgtgagccaa actcaattga ttactcactc tactcttgaa
     2221 ggaccaatcg aaaaacttct tggttggaca attggtaaca ctacaactgg tgatatcaaa
     2281 gcaatccttg gtgcagtagt cttccttgta ttctatatcg gtatctttgc ttggtacaga
     2341 aaacaaatga tcaaacgtaa cgaagagtac gcagcaaaag caaaataatg cgctcctata
     2401 aaatataaac ttaatgaaaa tcaaagagca aactaggaag ctagccgcag gttgctcaaa
     2461 acacagtttt gaggttgtag ataa
//
LOCUS       DAVKJU010000113         2433 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVKJU010000113 DAVKJU010000000
VERSION     DAVKJU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.113"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..391)
                     /locus_tag="VW191_001982"
     CDS             complement(<1..391)
                     /locus_tag="VW191_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624691.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCMGDFPHDFFDIYLDHVAKYAYE"
     gene            complement(405..1118)
                     /locus_tag="VW191_001983"
     CDS             complement(405..1118)
                     /locus_tag="VW191_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEU9624692.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1608..1715)
                     /locus_tag="VW191_001984"
                     /pseudo
     CDS             complement(1608..1715)
                     /locus_tag="VW191_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2137..>2433)
                     /locus_tag="VW191_001985"
     CDS             complement(2137..>2433)
                     /locus_tag="VW191_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEU9624693.1"
                     /translation="TPREEKPQSEKPESPKPTEEPEEESPEESEEPQVETEKVKEKLR
                     EAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLKENK"
BASE COUNT          766 a          461 c          381 g          825 t
ORIGIN      
        1 gttcgtaggc atattttgct acatgatcta ggtatatatc aaaaaagtca tgaggaaaat
       61 ctcccataca gccattacga ttcatattga ggctatattt tcgattagta agataattcg
      121 gaaatatatt tcctattact ctaagattat ctacttgtga atcccttctc ccaatataat
      181 ccccttcttt gactaatgat ggagatttcc aagtaaaaat taaatcgcca gagaaatttt
      241 tcttatcaaa aaacttaaaa agagattgat aaatactatt tgctaattga caagtgtcag
      301 atgtaaattc ataattaaca gcttcattaa aatcaacttc agacatttca atgtactctt
      361 caatagtaat attagctttt tttgtagtca ttagctaatc tcctttaaaa tgtgatcttt
      421 tcaagaatat ttttatctaa acaatccagc aagtcttggt aagaatagac ttcgtaagtc
      481 ggctgggctt gtgtgtgatt ttcgaggtga tgaggattat accagatagt gtcaatcccc
      541 gcattattgc caccttgaat gtcggcggtt agagaatctc caatcatcag cgtcttttct
      601 ttactaaatc cagcaatttg ctggccaatc ttttcataaa agagagcatc cggcttttga
      661 gtttgcaact gttctgagat aaagacttga ttgaaataag gtactagacc agattgagcc
      721 aaacgtcctg tctgaatggc agtaatgcca tttgtcgcag catacaagtc ataatcacgc
      781 tcaatgaggc tgtccaagag atcatgagcg cccgatagtg tttgtccctg ctgagcgaga
      841 taaaattggt aacgctgggc aagaaaacta ccgtcttttt cctgtccaaa atgagaaaat
      901 aaacgagaaa agcgcgtgtt aaccagctct tgtttactga ttttcttcag ctccaagtct
      961 ttccagagag ccttgttcat aggaacgtaa taatctttat aagcctgaat atccgcaact
     1021 ccttcttctt ttagaagttg ggtcaaagcc acatcctcag cagcatcaaa atcaagaaga
     1081 gtgtggtcga ggtcgaagag tagaaatttg tagaacaatt tgaggttttc ctttctgaaa
     1141 attcattaag aacattatat cataaagcac ctcatacaat taactaattt aatcacttaa
     1201 aaaaaattcg aacactttct atacaactga cagctcaaat ctttcagaat agaacaatac
     1261 taactatcga gcaccccgtc ttcataaata tatgtgtaat tctagaccta gaattcctat
     1321 aaactaaatg ctttcatact cttccaagta attgattgcc ttaaatttta atttttgaag
     1381 gattctaaag ctagaatagc cccatcacaa tcagttttga ttgattcaca atttagaaac
     1441 actatagttt cactcctgtt aaaataaaaa ggaactgcat aaagcaatcc ctttctgatt
     1501 ttgaaatcat ttacttaaca ttttatagtt gagataatca atagcttacc tataaaaaga
     1561 gttatagtaa aattccttat ttattgattt caagctccgc taactgtata agtaatcgga
     1621 acatctttag ctactttact aatcagttca acaacacctt ctgcaatgtg tttggaatgt
     1681 gaaacgataa caagaccaat accaatactt tccatcaaac caccccagtt tctaaaatag
     1741 cagtaaagag taatcctgat gagaatgatc caggatcaat atgtccaaga aaccacatgt
     1801 tcctaagaca agagctaaca gactggccgt caataatagt attgttcttt ttttcatcat
     1861 tactccttaa ctagtgttta actgattaat tagccagtaa atagtttatc tttatttaca
     1921 ctatctgtta agatatagta aaatgaaata agaacaggac agtcaaatcg atttctaaca
     1981 atgttttaga agtagaggta tactattcta atttcaatct actatatttt gcacattttc
     2041 ataaaaaaaa tgagaactag aactcacatt ctgctctcat ttttcgtttt cccgttctcc
     2101 tatcctgttt ttaggagtta gaaaatgctg ctacctttac ttattctcct ttaataaagc
     2161 caatagtttt tcagcttctg ccataatagt attgttgtcc tgggtgccaa atagtaaatt
     2221 attttttaat cctgtgagag tctctttggc attggacttg ataattggat cctggatttt
     2281 tccaagtaaa tcttcagcct ctctcagttt ttctttaacc ttttcagtct cgacctgagg
     2341 ttcttctgat tcctctggtg attcttcttc tggttcctct gttggttttg gagactctgg
     2401 tttctcgctt tgcggtttct cttctcgagg ggt
//
LOCUS       DAVKJU010000114         2404 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVKJU010000114 DAVKJU010000000
VERSION     DAVKJU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.114"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..246
                     /locus_tag="VW191_001986"
     CDS             <1..246
                     /locus_tag="VW191_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624694.1"
                     /translation="GIIYDNEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVV
                     INEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIKG"
     gene            560..1537
                     /locus_tag="VW191_001987"
     CDS             560..1537
                     /locus_tag="VW191_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624695.1"
                     /translation="MKNKHYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            1914..2288
                     /locus_tag="VW191_001988"
     CDS             1914..2288
                     /locus_tag="VW191_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HEU9624696.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
BASE COUNT          815 a          311 c          428 g          850 t
ORIGIN      
        1 ggaatcattt acgataatga agcattaaga gatttgtcaa aacaatattt aaacaaaaat
       61 atttcggaat tacaagatgg ttctttcgag ggcggtatac aagatgctgt tgcctctaaa
      121 caaatagtag ttataaatga atttatagct tgtttaaatt ccagatgcgt gccatcccaa
      181 tatgatagat ttttttataa agatagacct tatatttttt ccagaaagag tcctattaag
      241 gggtgaaaat ttgttacatt tggattaaca agtgtcctag ggattagaaa taactctagt
      301 atagctagat atttttgagt aactagaaat atcattagtc tacccatatg attgccagaa
      361 aagatggctg atgaatctta tttatactgt tgcagtaggc gttgtctagg ataaataagc
      421 tatcttgatg ggggagattg gaataatttt agtagaaaca ttaaaggatt atggacaagt
      481 agcgcaggat taggtgatgc aatgtgctaa gttggcttgg tgttagcagt agtattattt
      541 aacaatggaa agtagactaa tgaaaaataa gcattatttt tttgacacaa ttttaataat
      601 tttactgctt ataagcacga tattttgtgt cagcccagta tttataaaat tggacatctt
      661 aggaactccc tctcatgcca tcttaacctt tgtattggcg ataccgttat tctatatttt
      721 gtctcaatgc ttacatactt tgctattact agtttcctca atcttttgta aattaagacc
      781 aatttatttt tattttatat ttgtgatcat tataggagct aggaaatact atcgaattct
      841 ctttcaccaa ctaatgggat ttagtccagg aatagcagtg ttttataagg agagtcaaac
      901 aactaagaat ctgtttaaat tttactactt cttgtacttt acaacactaa tttcatacta
      961 ttttttcttt acttttgtgt atgacaaacc gttgcttttg cctcttattt cattttctat
     1021 tatcatagca ttagtacaaa aactttatag aattgagaat caacaattat ttctgttaaa
     1081 atcaaaggta ctaacaatac tggaatctaa gaagaattgt gagtttaatc ttcaggatta
     1141 tcatgaaatt tggaaattgc aatcaaaatc tgagttgcca tgcgtggctt tatcttatat
     1201 cagtttaatc aagccatatc tttcagagag tgtgcgtgaa caaattgatt tattagaagt
     1261 taagcgattt aaaaaaataa atcatccaat atcactttat ggtatgttgg atgttataaa
     1321 attaaatcta tatttacggc attataatga aaaaaataaa tatgaatcaa tgttaaagaa
     1381 aattttagag gtaagaccag actttgtttt aatcgagcag aatattgatg atagtttaaa
     1441 tagctcgcaa ccattatctt tatctttagc catttcagaa atccaacttt tgcttgaagt
     1501 gtatatgggg atcaaacatg tttcaattag gagataaaac cagaataata tttgccatta
     1561 taggggcaag ttgcatatta gtcagtatgt atcttattcc acttattaaa actgagaata
     1621 aattttggga aatactttta tttatattag gtgtattgct aattggaatt gcgttattaa
     1681 gtcaaaaaaa ataaattttg aaatagtatt agtgagttcg caatcattta ttgctagtgg
     1741 tttagaactg ttaagtttca aacaataatt gattttaaac cttaggtaac ctataaaaac
     1801 aaataaggta gagaaaggat atcttatcta ggtagctaag tgataaaaac acagtcgtct
     1861 atcacttgtt aggaaactat cctaactatg cttaataaaa aaggagaaaa taaatggaac
     1921 tcgtattacc aaataattat gttgttcttg agcaagaaga gatgatgtat cttgatgggg
     1981 gattttctat tccgagatgg cctgttgcaa cagccattaa tatagctttt aatggtgttt
     2041 taggtggagg agcaatcagt ctagttagaa attatattcg taattatggt ttgcggcgag
     2101 ttacaagcgc aattgctgga gcagctgcaa gatatgttgg ggtacgagtt gcaaatagag
     2161 tggcaggatt tgcactgtct gctattaatg gatttgcagc ttggatgtca attggcaatg
     2221 ctattacaac aatctgggcc aataatgatg taaataggag agacccaaat ttaaacgcct
     2281 tgtggtaact agatatgggt attaaaatgg aaaaaatttt tgttattatt ttttttgtat
     2341 gtctatttat atcatccata acttttttag cctatgattt tgttagcgaa gaactaaaaa
     2401 agtt
//
LOCUS       DAVKJU010000115         1999 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVKJU010000115 DAVKJU010000000
VERSION     DAVKJU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.115"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..155)
                     /locus_tag="VW191_001989"
     CDS             complement(<1..155)
                     /locus_tag="VW191_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU9624697.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEASHVFQISRNTIYGWLKLKEK
                     TGELNHQV"
     gene            281..688
                     /locus_tag="VW191_001990"
     CDS             281..688
                     /locus_tag="VW191_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU9624698.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQTDEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            853..1152
                     /locus_tag="VW191_001991"
     CDS             853..1152
                     /locus_tag="VW191_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEU9624699.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1261..>1999)
                     /locus_tag="VW191_001992"
     CDS             complement(1261..>1999)
                     /locus_tag="VW191_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEU9624700.1"
                     /translation="DQELKAEIQSIFIEHKGNYAYRCIHLELRNRGYLVNHKRVQGLI
                     KVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQK
                     LYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDS
                     YHQFLEGKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSLKQLEQAIVDY
                     IDYYNNKRIKVKLKGLSPVQYRTKSFE"
BASE COUNT          631 a          383 c          351 g          634 t
ORIGIN      
        1 acttggtggt ttagctctcc tgttttctct tttagcttta accagccata aatggtatta
       61 cgtgagattt ggaaaacgtg tgatgcttct gttatactac ctgttcgctc acaataagag
      121 agaacttttt tacgaaaatc tattgaatat gccataagaa gattatacca cattgtgtac
      181 tatttttggt tcattttact ataataataa cgatactaga taatgaggaa aatgcaatgg
      241 caaacccaac tctcggagaa aaaaagcgaa tacagactat attgctcgct atggcgtata
      301 tgcagttatc cctaatcctg aacaaaaaca aattgttctt gttcaagaac caaatggtgc
      361 ttggttccta ccatgtggag aaattgaagc aggtgaaaat catcaggaag ccctaaagcg
      421 tgagttgatt gaagagcttg gtttcacagc agaaattggt acctattacg gacaaactga
      481 cgaatatttc tattctcgtc atcgtgatac ctactactac aatcctgcct acctctatga
      541 agcaacttct ttcaaagaag tacaaaagcc actagaaaac tttaatcata ttgcctggtt
      601 ccctattgac gaggctatcc aaaaccttaa acgtggtagc cataaatggg ccattgaatc
      661 ttggaaaaaa cagcataaga ttggctaata ctcttcgaaa ataaaattca aaccacgtca
      721 gcgtcgcctt accgtactca agtatagcct gcggctagct tcctagtttg ctctttgatt
      781 ttcattgagt ataaatcaag cgactttatt caaaaagtgc tataatagaa ttagaaaatc
      841 ggaggaaata ttatgaagct tatcaatacg acaaactcac actcgcaact tgtaaaaagt
      901 caactagaaa gtaccgatgc aacccttgtt gaggtttatt ctgcagggaa tacagatgtt
      961 atttttaccc aagctccgct tcactatgaa atcctcatct caaacaaaca ccgtgctatt
     1021 cgtgagcctg aaatcgaaac cattcaagaa tttttcttga aacgtaaaat tgataaggcc
     1081 tctgttgatg aagccaatat caagacactc tactcagaga aattaattgg aatttcaatc
     1141 ccaatcaagt aagaaaattg agtaaaaact cgatttgaga gaaagtccaa taagtctttc
     1201 aataataaaa cgcatagtag caagagtttt gtactgaccc caaaaagtta gacaattaat
     1261 ttattcgaag gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat
     1321 tcgtttattg ttgtagtaat caatatagtc tacaatggct tgttccaatt gcttaagcga
     1381 ctgaaacgac ttctcataac cgtaaaacat ttccgatttc agaatcccaa agaaggactc
     1441 catcatacca ttgtctgggc tgttaccctt acgtgacatg gatgcttgaa ttcccttacc
     1501 ctcaagaaac tgatgataag aatcgtgttg atattgccag ccttggtcac tatggagaat
     1561 cgtattctcg tagtgcttct ctgtgaatgc ctgttccaac attgtttgta cttgttctaa
     1621 gttgggtgaa gttgaaagat tataggcgat aatttcgcta ttaaagccat ctaaaactgg
     1681 tgataagtaa agcttttgag tacttgctgg aatggcaaat tctgtcacat ctgtgtagca
     1741 cttttccatt gttttagagc cttcaaattg gccttgaatg agattctctg ctttcttacc
     1801 aacgtctcct ttatgagaag aatattttcg tttttgtcgc attttagctt gtaaattgag
     1861 tacttttatc aagccttgaa ctcttttatg atttaccaga taaccacgat ttcttagttc
     1921 taaatgaata cagcgataag cataatttcc cttgtgttcg ataaaaatgg attgaatttc
     1981 agctttaagc tcttggtcc
//
LOCUS       DAVKJU010000116         1919 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVKJU010000116 DAVKJU010000000
VERSION     DAVKJU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.116"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..165
                     /locus_tag="VW191_001993"
     CDS             <1..165
                     /locus_tag="VW191_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU9624701.1"
                     /translation="VSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSGVTLITG
                     KNGTGKSTLL"
     gene            complement(531..1841)
                     /locus_tag="VW191_001994"
     CDS             complement(531..1841)
                     /locus_tag="VW191_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU9624702.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVAITNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
BASE COUNT          605 a          318 c          453 g          543 t
ORIGIN      
        1 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
       61 gtggatcatc gtatattgtt tgataatatt tcttttgata cttcgagttc aggcgtgaca
      121 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat tgaaactaga
      181 atagtacaca tctacttcta aaatattgtt agaaatcgat ttgactatcc tgatctattt
      241 gtcctgttct tatttcattt cactatatct caaattgagt atgacgaagt gcgctcccat
      301 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
      361 tgggatgaat ttttaaagtt gaactaatta tttttacagg atgaggttta cagcagagag
      421 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
      481 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataatgaat ctaacttaag
      541 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca
      601 cccagaataa tttgcttttt gaaggcaagg ccgcgttcct ctatattggg aagtgagagt
      661 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
      721 gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat gatagcaatg
      781 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcactccg
      841 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt catgaggtgc
      901 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
      961 agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt ccctaatttg
     1021 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgtttttcga
     1081 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
     1141 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
     1201 ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtggct gtaacaggat
     1261 aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt
     1321 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
     1381 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacggcttg agctccaatc
     1441 aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata
     1501 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
     1561 tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
     1621 gtaccgttag ttattgcaac gttataaaag agagagacgc taaaaatggt aaaaaagagt
     1681 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
     1741 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
     1801 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
     1861 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggta
//
LOCUS       DAVKJU010000117         1870 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVKJU010000117 DAVKJU010000000
VERSION     DAVKJU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.117"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..1049
                     /locus_tag="VW191_001995"
     CDS             <1..1049
                     /locus_tag="VW191_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEU9624703.1"
                     /translation="KKRRVLLKSYNAETISCNPKYIYNELKKINRYELVWVLKEPEKY
                     PDVKAVKYMSLKWLYYIVTSQYVISNTGFNFWLPKKKKQIYINTWHGGGAYKDSDNLN
                     NISKAQVKQIKKSAELEDYFISSCAVFTKEYAKKRVGFKGKILEIGTPRNDFLIRNQA
                     DDGLKNSIKNRLGIDDNCSIVIYAPTWRDDASAIEEIDYESLLESLRETYPKVSILVR
                     GHHLQKNLIDIKNSNIVNVSDYDDMQELLLISDLLITDYSSTIWDMIHGGKKVLLYTP
                     DLDEYLKYRGFHVDIKEWNIPYFKTNEELIAYISSNYFKNMEEMIENHKIRFGSFEHG
                     NATQKIVELLEEGN"
     gene            1050..>1870
                     /locus_tag="VW191_001996"
     CDS             1050..>1870
                     /locus_tag="VW191_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU9624704.1"
                     /translation="MKVSVIIPVYNVEKYLRRCLDSVVNQTYKDIEVILVNDGSPDNS
                     KEICEEYVAKYSNIQLINQKNAGLGAARNTGLQYITGNAVTFVDSDDWLELDAIEYYV
                     ASMKKSDADIVVTQMIRKKEYFSNEGTNGTTIKEEVLNQEQFAKKYFKIDGNNIEYYA
                     CAKLYKREIAREVKYPVGLFAEDVPAAFGYIIRSQKIFYSTKVTYNYFFNDNSLTAKF
                     TDKDFDLEKIWDLVVEEAKVYGNEDYILYAKVNRYRIDFNLLCRIALSENKSDIE"
BASE COUNT          738 a          190 c          355 g          587 t
ORIGIN      
        1 taaaaaaacg ccgagttctg ctgaaaagtt ataatgcaga gactatttct tgcaatccaa
       61 agtatattta taacgaactt aagaaaatta atcgttatga attagtgtgg gtgttgaagg
      121 agccagaaaa atatcctgat gtaaaagcag taaagtatat gtcgttaaaa tggttgtatt
      181 atatagttac ttctcaatac gtaatatcaa atacgggatt taacttttgg cttcctaaaa
      241 agaaaaaaca gatatatata aatacatggc atggaggtgg agcatacaaa gatagtgaca
      301 atttaaacaa tattagtaaa gcgcaggtca agcaaattaa aaagagtgca gagttggaag
      361 attattttat atcgagttgt gcagtcttta caaaggaata tgcaaagaaa agagtaggat
      421 ttaaaggtaa aatactagag atagggacac ctcgaaatga ttttttaatt cgtaatcaag
      481 cagatgatgg tctaaaaaat agtataaaaa atcgacttgg aatagatgat aattgttcta
      541 tagtaattta tgctccaaca tggcgagatg atgcttcagc tattgaagag atagattatg
      601 aaagcttact agaaagttta agagagacat atccaaaagt gagtatttta gtacgtggtc
      661 atcatcttca aaagaatttg attgatataa agaattcaaa tattgttaat gtatctgact
      721 atgatgatat gcaagaatta ttattaattt cagatttatt aattactgat tactcttcaa
      781 caatatggga tatgattcat ggagggaaaa aagtattatt atatactcct gatttagatg
      841 aatatttaaa atatcgagga tttcatgtag atataaaaga atggaacatt ccatacttta
      901 aaactaacga agagttgatt gcatatatct cttcaaatta ttttaaaaat atggaagaaa
      961 tgatagagaa ccataagatt agatttggaa gttttgaaca cggaaatgca actcaaaaaa
     1021 tagtggaatt attagaagaa ggtaattaaa tgaaagtaag tgtaataata cctgtttata
     1081 atgttgaaaa atatttaaga aggtgtttgg attccgttgt taatcaaaca tacaaagata
     1141 ttgaagtgat tttggtaaat gatggttctc cagataattc aaaagaaata tgtgaagaat
     1201 acgttgctaa atattccaat attcaattaa taaatcaaaa gaatgcaggg ctaggtgcag
     1261 caagaaatac tggtctacag tatataacag gaaatgcggt aacttttgtc gattctgatg
     1321 attggttaga gttagatgca attgaatatt atgttgcatc tatgaaaaag agtgacgcag
     1381 atattgtagt tactcaaatg attagaaaaa aagaatattt ttctaatgaa ggaactaatg
     1441 ggacgactat aaaagaagag gtcctaaatc aggaacagtt tgcaaaaaaa tattttaaaa
     1501 tagatggtaa taatatagag tattatgctt gtgcaaaact ttataagaga gagattgcta
     1561 gagaggttaa ataccctgtt ggtttatttg cagaagacgt ccctgcagca tttggttata
     1621 ttatacgttc acaaaaaatt ttttatagta caaaagtaac gtataattat ttctttaatg
     1681 ataatagttt aacggctaaa tttacagata aagattttga tttggaaaag atatgggatc
     1741 tagtcgtaga agaagcaaag gtttatggga acgaggatta tattttatat gccaaggtga
     1801 atagatatag aatcgatttt aatcttttgt gcagaattgc tttatcggaa aataaatctg
     1861 atatagaaaa
//
LOCUS       DAVKJU010000118         1591 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVKJU010000118 DAVKJU010000000
VERSION     DAVKJU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.118"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>1591
                     /locus_tag="VW191_001997"
     CDS             <1..>1591
                     /locus_tag="VW191_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU9624705.1"
                     /translation="VYRNGSDFSVIIVNCLLAVVCKKINSSSTKLLPEFSGLDFSLWQ
                     DYIQSTGSIKELWPSQIELGKQGIFSGKSGIVQMPTSSGKTASINLTLRSAFYSNRID
                     NALIVAPFRALCREIYRDINAHFVDENNVIVSEVFDLPEIPQDFSIFNDGKKRVFILT
                     PEKLLFLLRNHQSFIDEIGLCIFDEAHLFDDPSRGTNFELLLSTVKQIFPKGIQKILI
                     SAVIPNSEAINRWFNEDGVIVSNNSIKTTEKRVAFSDLNGSNEQLYFIDPITFEEEFF
                     VPRTVSVSELEQLGKERKQKVFPELTNANDISIYYGTKLINNGGVGIFCGRKDTVNVI
                     LRRFIDLNNRNYDLTDFLKNSDRSEVEKIGNLIGQNLGYDSVEYTCSQLGVFSHHSGI
                     PMGIRIAIEYAFSKSKINNVVCTSTLAQGINLPIKYLIISSVYQAGDAIKVRDFQNLI
                     GRAGRAGKYTEGTIILTEPNIYKSPKNKWKKQNYKALLNPINTEGCQSNILSIIQFKS
                     VVPTDYRFAPIKFDFWNLIKER"
BASE COUNT          586 a          189 c          292 g          524 t
ORIGIN      
        1 agtatatagg aatggaagcg atttttctgt aattattgta aattgtctct tagcagttgt
       61 atgtaaaaaa ataaatagtt catcaacgaa attattacct gaattttcag gattagattt
      121 ttctttatgg caggattata tacaatcaac aggaagcata aaggagcttt ggccatctca
      181 aatcgaatta gggaagcaag gaattttttc aggtaaatct ggaatcgttc agatgccaac
      241 aagttcgggt aaaacagcct caatcaatct aactttaaga tcagcatttt attcaaatag
      301 aattgataat gcattgattg ttgcaccttt tagagcatta tgtagagaaa tatatcgaga
      361 tattaatgca catttcgttg atgagaataa tgtgattgtt agtgaagttt ttgatttacc
      421 ggagattcca caggatttct caatttttaa tgatggtaaa aaaagagttt ttatacttac
      481 tccagagaaa ttgttgtttt tattgagaaa tcatcaaagt tttattgatg aaataggact
      541 ttgtatattt gatgaagcac atctttttga tgatccaagt cgtggaacca attttgaatt
      601 actgttgtca acggttaaac aaattttccc aaaaggaata cagaagattt taatatctgc
      661 tgtaatacca aatagtgaag ctataaatcg gtggtttaac gaagatggag taattgtttc
      721 taataattcg attaaaacaa cagagaaaag agttgcgttt agtgatttaa acgggagtaa
      781 tgagcaatta tatttcatag atcctattac atttgaagaa gaattttttg ttccacgtac
      841 tgttagtgtt agtgaattag agcagcttgg aaaagaacgc aaacaaaaag tttttccaga
      901 actgacaaac gcaaatgata taagtattta ttatgggact aagctaatta ataatggtgg
      961 agtaggaatt ttttgtggaa gaaaagacac ggtcaatgtt attttacgaa gatttataga
     1021 cttaaataat agaaattatg atctaactga tttcttaaaa aactcagata ggtctgaagt
     1081 tgaaaaaata ggaaatttaa ttggtcaaaa tttaggatat gatagtgttg aatatacatg
     1141 tagtcagcta ggagtttttt cacatcattc aggtatacct atggggatac gcattgctat
     1201 agagtatgct ttttcaaaaa gtaaaataaa taatgtagta tgtacttcaa ccttagcaca
     1261 aggtataaac ttacctatta aatacttgat tatctcaagt gtgtatcaag caggagatgc
     1321 aataaaagtt cgtgattttc agaatttgat aggtagagca ggaagggctg gaaaatatac
     1381 agaaggaaca ataattttaa cagagcccaa tatttataaa tcaccaaaaa ataagtggaa
     1441 aaaacaaaac tataaagcat tgttaaatcc tattaataca gagggttgtc aaagtaatat
     1501 tctcagtatt atacagttta aatcagtagt tccaactgat tatcgattcg ctcctataaa
     1561 atttgatttt tggaatttga ttaaagagag a
//
LOCUS       DAVKJU010000119         1566 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVKJU010000119 DAVKJU010000000
VERSION     DAVKJU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.119"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..267)
                     /locus_tag="VW191_001998"
     CDS             complement(<1..267)
                     /locus_tag="VW191_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624706.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVL"
     gene            complement(264..617)
                     /locus_tag="VW191_001999"
     CDS             complement(264..617)
                     /locus_tag="VW191_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU9624707.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAEKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(673..1545)
                     /locus_tag="VW191_002000"
     CDS             complement(673..1545)
                     /locus_tag="VW191_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU9624708.1"
                     /translation="MLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSLTFFVLILSKI
                     VLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSPDDLRSRIIWS
                     FILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQNKWQKEDSFES
                     DLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKIEDSHNTLLCT
                     GLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNFDFCMNREYHK
                     MKSRKLLLSNKKIV"
BASE COUNT          574 a          277 c          210 g          505 t
ORIGIN      
        1 taatacggta taagtcaaaa aactttctgg gaaaaactta ttgattaaaa tggggaaact
       61 gattaacaga atcgaattta tggctagaca gatagatact attttatttt tcacagaata
      121 tagaggtagt gatactattg agtagataaa agctaaaaaa ataagcaaaa aatactccgt
      181 atcatttata atcgccctga atgataaaaa tatatacacc caacacacga tgatgtgtat
      241 ttttcgaatc ttatctacat tactcatctt actgttgcag atatgaatcc atcccaagga
      301 taagggttcc cgttaatatc acaacctcta ttaagaacac catatccaat acagtaagct
      361 attttttctg ctgctgtagc caatacacct gctgctgcac caataagcca gcctccaaga
      421 cctccaaact taatatagtt tattagtttt ttggttaaaa ctgcagctcc agctaatgct
      481 ataaaagttc ctgaactcat agctaaagct gcgcaaattc cttgacaagc acgcttgctt
      541 aaataagctc ccccatcaag atacatcatt acttcttcat caatcacaac ataattattt
      601 ggtaatacaa gttccatttg ttttctcctc cttagttgaa attgtttcat ttgttttaca
      661 aataggacga acttaaacta tctttttatt actcagtaat aatttccgag atttcatttt
      721 atgatactct ctattcatac agaaatcaaa attttgcctt aaccaacttg tataatagaa
      781 aaaatctaat tgattaatct tgataagctc tatcctaatt ttatcaatct tttctgacca
      841 attatgattc ttactaatag aaatttcata cagcagtaac tcgacaagac ctgtacacag
      901 aagggtgtta tgagaatctt cgattttttt caaaatatcc tcaaaaagtt cactatccgt
      961 taaaaaggtg gattggttca tcgcatcata gagaagaagg gacattaagt cagaatagct
     1021 taattctgat ttttgtatct tatcctttaa cgttttcgtc aaatcacttt caaacgaatc
     1081 ttctttttgc catttgtttt gattaaaaag aaattccgtc ttattaaaaa cgagatgatt
     1141 gatatccatt atagtccaga aaaatattgt agtaagcatg atgattgcaa aaggaatttt
     1201 taaaataaat actaggataa tagcaatttc aagaataaaa ctccatataa tcctagatct
     1261 taaatcatca ggcgacaaac ttttacgatg gaatgaaaaa tagggcgaga acctcacatt
     1321 tactttcttc ctgcctataa aaataaaagg aaaaatcgta ataagagctt ctttcttgta
     1381 caaatctttc aatgcgtata gtatgtctaa aactattttt gataatatca aaacaaaaaa
     1441 agtaagactc gtactaacca caaaatcaaa gggaatttct agattcaatt gcaagttact
     1501 cactaacaaa tgcaactcta gccacaagac aaaaagaggg agcattaata aaaaaatgat
     1561 tagttt
//
LOCUS       DAVKJU010000120         1562 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVKJU010000120 DAVKJU010000000
VERSION     DAVKJU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.120"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..653
                     /locus_tag="VW191_002001"
     CDS             <1..653
                     /locus_tag="VW191_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9624709.1"
                     /translation="EKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWV
                     RLNDITSYIQRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLL
                     RDGDLMWNSTGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSS
                     PIVQSVIEEKASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            710..1507
                     /gene="psrA"
                     /locus_tag="VW191_002002"
     CDS             710..1507
                     /gene="psrA"
                     /locus_tag="VW191_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEU9624710.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          546 a          223 c          284 g          509 t
ORIGIN      
        1 cagaaaaaca aaaactcttt gaagaaggca agattaaaaa gaaagatttg gacatttcta
       61 ttgtttccca aggagatgat aactcttatt atgaggaagt accttgtgaa ataccagaaa
      121 gttgggaatg ggtgaggtta aacgatataa cctcctacat tcaaagaggg aagtctccaa
      181 aatattcaaa tattcctata taccctgtga ttgctcagaa atgtaatcaa tggtctggct
      241 tttctattga cttagcaaga tttattgatc ccgaaacagt tcatagttac cagaaagaaa
      301 gattactaag ggatggtgat ttaatgtgga attcaacagg tctaggtacg ttaggacgat
      361 tagctattta ccatgaaaat aaaaatcctt atgcctgggc tgttgcagat agccatgtta
      421 cagttataag agtcttatcg ggggttatta attgccattt catctataat tttctttcct
      481 ctcctattgt tcagtcagtt attgaagaaa aagcgtcggg tagtacaaaa caaaaagaat
      541 tattaacgaa aactataaaa gaatatttaa tccccctccc accccttcct gaacaatcca
      601 gaatcgttga taaaatcgaa caatttttcg cccatattga cgcactaatt tagtataata
      661 gttttacgtg cggtggaatt tctatcaaac ataagatagg agttttacgt tgaaagattt
      721 tgaattacat ttgaagaaag ctggtttagc tgaaaatact gtgcgttcgt acctttatgg
      781 tgttcgattt ttcttggaga attacgaact caaaatggaa gatttatttg aatacaaagg
      841 atatttatta gacaatttta aacctaaaac tgtcaatttg cgactacaag gagtaaataa
      901 atatctggct tttattggtc atgatgattt gaaattaaaa tttgtcaaag tacagcaaaa
      961 accgttttta gaagatgtca ttagccatgc tgattacctt tttcttaaac gtagtttgaa
     1021 aaaagatggt attttaaaat ggcattttgt cgtttggttt ttaggtgcaa ctggggctcg
     1081 tgtaagtgaa cttattaaac taaaagtaga gcatgtagaa atcggttatt ttgacattta
     1141 ctcaaaaggt ggtaaaattc gtagactgta cattcctaaa aaattaagaa atagttgtct
     1201 tagttggtta gaatcagaaa atcgccgaag tggttattta tttttgaata aatttaacga
     1261 accaattaca gcaagaggag ttgctcaaca gttaaaaaat tatgctgata aatacaaaat
     1321 gaatcctaaa gtaatttacc ctcattcttt taggcattta tttgctaaga attttttagc
     1381 gaagtataat gatattgcct tgcttgcaga tttgatggga cacgaaagta tagaaactac
     1441 tcgaatttat ctaaggaaaa cagctactga acaacaaaat attgtagata aaattgttaa
     1501 ttggtaaaaa ataacaggtg gtcaaactga ctacctgcta tttttgtgat tatggctctt
     1561 at
//
LOCUS       DAVKJU010000121         1484 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVKJU010000121 DAVKJU010000000
VERSION     DAVKJU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.121"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            27..1373
                     /gene="gdhA"
                     /locus_tag="VW191_002003"
     CDS             27..1373
                     /gene="gdhA"
                     /locus_tag="VW191_002003"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEU9624711.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          447 a          303 c          317 g          417 t
ORIGIN      
        1 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
       61 ttgaaactgt aaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      121 ttttcaacac tttggaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      181 tggcacgtat tactgagcct gagcgcgtga tttctttccg tgttccttgg gttgaccgtg
      241 atggaaaagt tcaagtaaac cgcggctacc gtgttcaatt caactcagct gttggaccat
      301 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      361 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      421 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      481 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      541 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      601 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      661 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
      721 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatacgct cttcaaaaag
      781 caaccgaact tggtgcaaca gttatctctg tttctgactc aaacggctat gttatcgatg
      841 aaaacggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgc gctcgtttga
      901 ctgagtatgc agctgagaaa gcaactgcta cttatcatga aggttctgta tggacttacg
      961 ctggaaacta tgacattgcg cttccatgtg ctactcaaaa cgaaatcaac ggtgaagcag
     1021 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccaagca
     1081 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gccaaagctg
     1141 ccaacgctgg tggtgtagcc gtttcagccc ttgaaatgag ccaaaacagc cttcgcctct
     1201 catggactcg tgaagaagtt gatggacgtc tcaaagacat catgaccaac atctttaaca
     1261 cagctaaaac aacttcagaa acatatggtc ttgataaaga ctaccttgca ggagctaaca
     1321 ttgctgcctt tgaaaatgta gcaaacgcta tgattgcaca aggcgtggta taaaaaaaaa
     1381 ttacggaaac tgactaccca gacgggtagc cagttttttc aattcctaaa atttaagatt
     1441 ctaataccct ttatgcaaaa atatagaatt ctaccttttt cttt
//
LOCUS       DAVKJU010000122         1470 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVKJU010000122 DAVKJU010000000
VERSION     DAVKJU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.122"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            36..>1470
                     /gene="pcpA"
                     /locus_tag="VW191_002004"
     CDS             36..>1470
                     /gene="pcpA"
                     /locus_tag="VW191_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEU9624712.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNES"
BASE COUNT          576 a          200 c          239 g          455 t
ORIGIN      
        1 tatctattag aaaataaata tgtaaggaaa tttaaatgaa aaaaactaca atattatcat
       61 taactacagc tgcggttatt ttagcagcat atgtccctaa tgaaccaatc ctagcagata
      121 ctcctagttc ggaagtaatc aaagagacta aagttggaag tattattcaa caaaataata
      181 tcaaatataa ggttctaact gtagaaggta acataggaac tgttcaagtg ggtaatggag
      241 ttactcctgt agagtttgaa gctggtcaag atggaaaacc attcacgatt cctacaaaaa
      301 tcacagtagg tgataaagta tttaccgtta ctgaagtagc tagtcaagct tttagttatt
      361 atccagatga aacaggtaga attgtctact atcctagctc tattactatc ccatcaagca
      421 taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc taaaactatt atttttgaca
      481 aaggcagtca gctggagaaa attgaagata gagcttttga tttttctgaa ttagaagaga
      541 ttgaattgcc tgcatctcta gaatatattg gaacaagtgc attttctttt agtcaaaaat
      601 tgaaaaagct aaccttttcc tcaagttcaa aattagaatt aatatcacat gaggcttttg
      661 ctaatttatc aaatttagag aaactaacat taccaaaatc ggttaaaaca ttaggaagta
      721 atctatttag actcactact agcttaaaac atgttgatgt tgaagaagga aatgaatcgt
      781 ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac tcaattaatt tattatccaa
      841 gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggagac aaaagaactt gcatcatatt
      901 cgtttaataa aaattcttac ttgaaaaaac tcgaattgaa tgaaggttta gaaaaaatcg
      961 gtacttttgc atttgcggat gcgattaaac ttgaagaaat tagcttacca aatagtttag
     1021 aaactattga acgtttagcc ttttacggta atttagaatt aaaagaactt atattaccag
     1081 ataatgttaa aaattttggt aaacacgtta tgaacggttt accaaaatta aaaagtttaa
     1141 caattggtaa taatatcaac tcattgccgt ccttcttcct aagtggcgtc ttagattcat
     1201 taaaggaaat tcatattaag aataaaagta cagagttttc tgtgaaaaaa gatacatttg
     1261 caattcctga aactgttaag ttctatgtaa catcagaaca tataaaagat gttcttaaat
     1321 caaatttatc tactagtaat gatatcattg ttgaaaaagt agataatata aaacaagaaa
     1381 ctgatgtagc taaacctaaa aagaattcta atcagggagt agttggttgg gttaaagaca
     1441 aaggtttatg gtattactta aacgaatcag
//
LOCUS       DAVKJU010000123         1286 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVKJU010000123 DAVKJU010000000
VERSION     DAVKJU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.123"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..>1286)
                     /gene="lanM"
                     /locus_tag="VW191_002005"
     CDS             complement(<1..>1286)
                     /gene="lanM"
                     /locus_tag="VW191_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 2 lantipeptide synthetase LanM"
                     /protein_id="HEU9624713.1"
                     /translation="DKNGNIFSSLVFYLEDLIYPWIVKPLVLEINSLREKGLLEGESE
                     QQRYKYFITLFDKEENILNFYNKYPVLLRQISESCLRFYTYFIEILSNLENDFSVLEE
                     ELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKIVYKPKNLIINNSLNTIAEYIRKV
                     DEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKNLPEYYYNFGVLLAFIYLFNGSDIHF
                     ENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDTLFHRVTRTLLLPTEGVKREDGLD
                     VEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKIDFEGGKNLPVRDGDIEFDKYIKD
                     FKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTRVLFRDTNSYASVLSFLYHPDFYE
                     EMLDREKALENLWSNKFSNQGIVASECEQMRLLDIP"
BASE COUNT          438 a          247 c          144 g          457 t
ORIGIN      
        1 tcggaatatc taataacctc atttgttcac attcgcttgc tacaatacct tgattagaaa
       61 acttattgct ccagagattt tccaatgctt tttctctatc taacatttct tcataaaaat
      121 caggatgata taaaaaagat agtactgaag catagctatt tgtgtctcta aaaagtaccc
      181 ttgtctttaa accatacaag tttgctttta atagcatttt aaattcttct gttttattta
      241 actcttcaaa tatcagataa aaatccctaa aacctttttt gaaatctttt atatacttat
      301 caaattctat atcaccatcc cgaacaggca ggtttttccc accttcaaaa tcaattttcc
      361 caatatcaaa ctttacctta tcagtattta aattaattaa aacttgacca ttaaaagcat
      421 ccttcttaaa atttcctgta agagcactca tctcaacatc aagaccatcc tcccttttta
      481 ctccctctgt tggaagaagc aaagttcgag tcaccctgtg aaacagggta tccagcaaac
      541 tctgaccagt tttatcatca aatagaggtt gctgtaacat tgtttcaaag tctattatta
      601 caggcatatc tccataggaa attaaatttt caaaatgtat atcactccca ttaaataaat
      661 atataaatgc taaaagcaca ccaaaattat aataatattc aggtaaattt ttcttttgct
      721 ctagaggtag ataatcaata aattcttcat agctgtgatc cgaataagca atagttcgag
      781 gtattcttat cctaattttt tcatcaacct ttcggatata ctcagcaata gtatttagtg
      841 agttattgat tattaaattt ttaggcttgt aaacaatttt cgcgtcatca aagaagagta
      901 tcaaaacagt ttttccttgg ctgtgtgtat caccctttcc aaattttata tcatttaatt
      961 tcccccttag ccctaattct tcttctagca cactaaaatc attttctaaa tttgataaaa
     1021 tttctataaa ataagtatag aaccgaagac aagactccga tatttgcctc agtaaaacgg
     1081 gatatttgtt ataaaaattt aatatattct cttccttgtc aaacaatgtt ataaaatatt
     1141 tgtaccgttg ctgctccgat tccccttcaa gtaaaccttt ttcacgcaat gaatttatct
     1201 ctaaaaccaa aggtttaaca atccaaggat agattaaatc ttctaaataa aatactagag
     1261 aggaaaaaat atttccattc ttgtca
//
LOCUS       DAVKJU010000124         1182 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVKJU010000124 DAVKJU010000000
VERSION     DAVKJU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.124"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            231..>1182
                     /locus_tag="VW191_002006"
     CDS             231..>1182
                     /locus_tag="VW191_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9624714.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          411 a          206 c          248 g          317 t
ORIGIN      
        1 taccttagtc aagaattaga tgtttcacta tgtttgagta agttgatgat ttcatttgat
       61 aacaggtttg aaactgttag gctaggtggc caaggctaat catagccttg gtttagctga
      121 aaaaacaggt tcaagggttc ctgttgtcaa atgaaatgtg atttaaggta taagaaaaca
      181 cctctgtgct atacttgttg ttcaccacaa acacaaggaa aggcacagag atgcaagaac
      241 attatacccc aaaagggaaa catttgacaa tagataaccg tcgcttgatt gagcggtgga
      301 agaatgaaaa taagtccaat cgtgaaattg caggcttgtt aggaaaggcg cctcaaacga
      361 ttcataatga agtcaaaaga ggtacaactt tacaacaagt gagaaaaggg ctatacaaaa
      421 aggtctattc tgccgattac gcacaaactg tttaccaatt caatcgaaaa cggtcggtga
      481 aaaagttaat tttaacaaag gaaatcagag agaagatctt acactatcat aagcaaaaat
      541 tttcgcctga aatgatggtt aacaagaagc aagtgaaagt tggtatttca accatctact
      601 actggtttca taatggtcat ttaggattga cgaaggccga catgctttat cccagaaaaa
      661 ggaaaggtgt caagaagcaa gctagtccga actttaagcc ggcaggtaaa tctatcgaag
      721 aacgtcctga cgttattaat cttcgcttgg aaaatggtca ttatgaaatt gataccgtcc
      781 tactgactaa gataaaaaat tattgcctgt tagtcttaac cgaccggcgg agcagacacc
      841 aaattataag gttaattcca aataaaactg ctgaatctgt caatcaggcg cttacgttac
      901 tattagggga gcatcgtatt ctgtccatta ctgcagataa tggttcggag ttcaaacgat
      961 tgtctgaggt atttcctgag gaacatatct actacgcaca tgcttactct tcatgggaga
     1021 gaggttcaaa tgaaaatcat aatcgattaa ttcggagatg gttacctaaa ggaaccaaga
     1081 aaacgactcc gaaagaagta gcttttatcg aaaattggat taacaactac cctaaaaaat
     1141 gcttggacta caagtcgcca agtgaatttc ttttgggtgg ct
//
LOCUS       DAVKJU010000125         1151 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVKJU010000125 DAVKJU010000000
VERSION     DAVKJU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.125"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            2..730
                     /locus_tag="VW191_002007"
     CDS             2..730
                     /locus_tag="VW191_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU9624715.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGTLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
BASE COUNT          385 a          178 c          197 g          391 t
ORIGIN      
        1 aatgagtaca aaatatttat ttatttacaa tgagattcgt gaaaagattc tttgtaataa
       61 atataccatg aacgaacaat tgcctgatga aatgacatta gctaaacagt ttgcctgtag
      121 tcgaatgacg atcaaaaaag ctttagactt gttagtttct gagggcttaa tttttagaaa
      181 acgtgggcag ggaacctttg ttctctctcg tggcagctca aaaagaaaat taatcgttcc
      241 agaaagagat atccggggac tgacaaaaat atctgaagat gctcattcta caattgactc
      301 gaggattatt cacttcaaat tagaatttgc aaatgaattt ttagcagaaa aactacaggt
      361 cgctttgcag agtccagttt ataatattta ccgcctgcgt attattgacg gtaaacctta
      421 tgttctggaa caaacttata tgagtaccga tgttattcca ggtattactg aagatatttt
      481 acaaaaatcg atttacaatt acattgaagg aacgttagga ttgcatattg ccagtgctac
      541 aaaaatctta cgagcttctt ctagttcaga aaatgagcaa cattacttgc agctccttcc
      601 aacggaaccg gtatttgaag tagaacaagt ggcttatttg gataacggaa ctccgtttga
      661 gtactcgatt agtcgtcatc gctatgattt atttgaattt aattcttttg cattacgaca
      721 ttcctcctag gagaaaatgt gaaaatgaag ccaatctttt acagactcta gtttaagaaa
      781 aatttaaaac agggcaagaa ggtcccatct atgcttaaat ggtttctctt ttctaaataa
      841 gatggcttta aaagagtgat cgttttatcc atcatgttga aaaatatctt cgtatagctt
      901 atagagtagg tactgaaatt gttcacctga tctacttctt atagttattt agttttaaat
      961 agtgtttcaa atattcttac actgacgaga agtttttgag tcttttcttg taacacatat
     1021 agtatactga ggttagaata gtagactgtg acttctaaca aattgctaga aatgaatttc
     1081 aatctcccaa tttatttgtt caaatattgt tttaatatat tgaataaatt ctgaatcata
     1141 atcatttaaa a
//
LOCUS       DAVKJU010000126         1121 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVKJU010000126 DAVKJU010000000
VERSION     DAVKJU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.126"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            168..1040
                     /locus_tag="VW191_002008"
     CDS             168..1040
                     /locus_tag="VW191_002008"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU9624716.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          315 a          194 c          246 g          366 t
ORIGIN      
        1 ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac
       61 acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagta
      121 attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt
      181 caactgattt aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg
      241 ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt
      301 cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa agggttttta
      361 ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt
      421 tgactagtgg cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg
      481 aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt
      541 atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta
      601 atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta
      661 atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa
      721 aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg
      781 aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata
      841 atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg
      901 aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt
      961 acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc atgaagggtt
     1021 taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc
     1081 tagaacgagt tttatgtcag atagtttaaa cgcctcttca a
//
LOCUS       DAVKJU010000127         1116 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVKJU010000127 DAVKJU010000000
VERSION     DAVKJU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.127"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>1116
                     /locus_tag="VW191_002009"
     CDS             <1..>1116
                     /locus_tag="VW191_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEU9624717.1"
                     /translation="GTESLFKALEGYRKVFLPTISNNEWFKKQTKAYIVEEKSTIEEG
                     REKQGKEGTKYSIGGYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDR
                     YRSSEYPKGEKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLYRSILVYDAFKF
                     GTDKDKDKVTHQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKD
                     GAVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYE
                     DSENSTRLQVADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVANLETNQKYELLR
                     KIENKFDLDQDGNNVYATNVVRRLTMDEVNKLNSFDS"
BASE COUNT          450 a          137 c          206 g          323 t
ORIGIN      
        1 atggaactga aagtttattt aaagcattag aaggatatag aaaagtattc ttaccaacaa
       61 tttctaacaa tgagtggttt aaaaaacaaa caaaagcata tattgtagaa gaaaaatcta
      121 caattgaaga gggaagagaa aaacaaggaa aagagggaac taaatattct attgggggat
      181 atgatagatt aactaatcca tcttggaaat atcaaagtat ggtcttacca ctattaactt
      241 tacctgaaga aaaaacagtg tttatgatag cgaatatttc tactattgga tttggagcct
      301 atgacagata tagaagttca gaatatccta agggagaaaa gttaaacaaa tttgtagaag
      361 ataacgccaa agaagcggct aaacgattta gagatcatta tgattattgg tataagatat
      421 tagataacga taataaagaa aaattatata gaagtattct agtatatgat gctttcaaat
      481 ttggtactga caaagataaa gacaaagtaa ctcatcaagc tacttttgaa actgatcatc
      541 cagctataaa atacttcttt ggacctgcgg gaaataatgt tgttcataat ggacacggag
      601 catatgctac cggagatgca ttctattata tggcatatcg tatgttggat aaagatggag
      661 ctgtaacata tactcatgaa atgacacata actcagatag agagatttat cttggaggat
      721 atggaagaag aagcggatta ggacctgagt tttatgcaaa aggattatta caagctccgg
      781 atcatccata tgatccaaca ataacaatta actctgtctt gaaatatgag gattcagaaa
      841 attcaacaag acttcaagta gctgatccaa ctcaacgatt taatagtgca gaagacctac
      901 ataattatat gcataatatg tttgatgtaa tttatatgtt agaatattta gaaggaaaag
      961 ctgtagctaa tttagaaaca aatcaaaaat atgagttact aagaaaaatt gaaaataaat
     1021 ttgatcttga tcaagacggt aataatgtat acgcgacaaa tgttgttcgc agattaacga
     1081 tggatgaagt taataaatta aattcttttg atagtt
//
LOCUS       DAVKJU010000128         1086 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVKJU010000128 DAVKJU010000000
VERSION     DAVKJU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.128"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..160)
                     /locus_tag="VW191_002010"
     CDS             complement(<1..160)
                     /locus_tag="VW191_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEU9624718.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKF"
     gene            complement(173..922)
                     /locus_tag="VW191_002011"
     CDS             complement(173..922)
                     /locus_tag="VW191_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU9624719.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          378 a          211 c          115 g          382 t
ORIGIN      
        1 aaaacttcat aagctcttca tttttagatg gaagaatgcc cttttttaaa ttatttattt
       61 ttacagaatc tatatcatat gcaacaactt tatatttaga tgcaaatagt aacgcgtagg
      121 ccagcccaac atgccccaaa ccaattactg ctatattcat aaaactactt ccttatttct
      181 taatccaaaa tctaatagaa taagctgccc cattccttaa atacaactct ttaatattgt
      241 ttaaaagttt ttcaactgat ttccagatta tcaaaatctg agatttatag cacaatatcg
      301 atgatattct atcaatataa tgtttttcat caagttcctc ttgatacatt tttaattctt
      361 tagtttttcc catataacta accatactac tatcacttac atatgggaag tcctcataat
      421 atattacttt ataacgcata aattcaagcg cccttccaat actattcaca aaaacatgat
      481 caacatggtc accaagtgaa agcggacaat atacgacaca tttgtcgtct aaatgcatta
      541 acagctcttt tatgatatca ttctttaatg tgtcctcatt ttttaattca ctatagatat
      601 gacggtatag aaaattgcca tttctatctt tcctatagag acattcatag tacgataagt
      661 gtctaaaatc acattgtaga cgttcacaag ctaacctgtc ttctttcttc ctttcttcaa
      721 tcggatattt cccaaggtta cacaacttat gaaattgctt agcagagggc tgtagctgtt
      781 ggctcaaagg gtaaccagaa aatatagtaa taacaagtac aatttctcct tctgaagtta
      841 attttgaaat ataatcacca caggaaaaaa ttgcgtcatc taaatgtgga gataaaaaga
      901 tatacttagt attgttactc ataaccattc cctctacaat ttatctaaaa actcactaag
      961 tgtctgatta aattccacat catcaaaaaa attcacctta ttcttaataa tgaatatttc
     1021 gttaaataaa catatatata aatatttcaa tatcctttca atatcatcct ctaaattctc
     1081 ctcaat
//
LOCUS       DAVKJU010000129         1026 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVKJU010000129 DAVKJU010000000
VERSION     DAVKJU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.129"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>1026
                     /gene="phtB"
                     /locus_tag="VW191_002012"
     CDS             <1..>1026
                     /gene="phtB"
                     /locus_tag="VW191_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEU9624720.1"
                     /translation="PAPSNPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETA
                     AGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDLLDNKGRQVDFEA
                     LDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQVAKLAGKY
                     TTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKEKGLTPPS
                     TDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNI
                     KFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQAD
                     TNQTEKP"
BASE COUNT          340 a          202 c          226 g          258 t
ORIGIN      
        1 accagctcca agcaatccaa ttgatgagaa attggtcaaa gaagctgttc gaaaagtagg
       61 cgatggttat gtctttgagg agaatggagt ttctcgttat atcccagcca aggatctttc
      121 agcagaaaca gcagcaggca ttgatagcaa actggccaag caggaaagtt tatctcataa
      181 gctaggagct aagaaaactg acctcccatc tagtgataga gaattttaca ataaggctta
      241 tgacttacta gcaagaattc atcaagattt acttgataat aaaggtcgac aagttgattt
      301 tgaggctttg gataacctgt tggaacgact caaggatgtc tcaagtgata aagtcaagtt
      361 agtggatgat attcttgcct tcttagctcc gattcgtcat ccagaacgtt taggaaaacc
      421 aaatgcgcaa attacctaca ctgatgatga gattcaagta gccaagttgg caggcaagta
      481 cacaacagaa gacggttata tctttgatcc tcgtgatata accagtgatg agggggatgc
      541 ctatgtaact ccacatatga cccatagcca ctggattaaa aaagatagtt tgtctgaagc
      601 tgagagagcg gcagcccagg cttatgctaa agagaaaggt ttgacccctc cttcgacaga
      661 ccatcaggat tcaggaaata ctgaggcaaa aggagcagaa gctatctaca accgcgtgaa
      721 agcagctaag aaggtgccac ttgatcgtat gccttacaat cttcaatata ctgtagaagt
      781 caaaaacggt agtttaatca tacctcatta tgaccattac cataacatca aatttgagtg
      841 gtttgacgaa ggcctttatg aggcacctaa ggggtatact cttgaggatc ttttggcgac
      901 tgtcaagtac tatgtcgaac atccaaacga acgtccgcat tcagataatg gttttggtaa
      961 cgctagcgac catgttcaaa gaaacaaaaa tggtcaagct gataccaatc aaacggagaa
     1021 acctca
//
LOCUS       DAVKJU010000130         1025 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVKJU010000130 DAVKJU010000000
VERSION     DAVKJU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.130"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..559
                     /locus_tag="VW191_002013"
     CDS             <1..559
                     /locus_tag="VW191_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9624721.1"
                     /translation="NKDETTSYPIYEIPESWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(507..>1025)
                     /locus_tag="VW191_002014"
     CDS             complement(507..>1025)
                     /locus_tag="VW191_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9624722.1"
                     /translation="DIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKPLEFSLLDNDYY
                     IDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIFQLTPFESSE
                     IISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFEEQELITQKV
                     EKLFEKVNQLWK"
BASE COUNT          369 a          162 c          152 g          342 t
ORIGIN      
        1 gaataaagac gaaacaacct cttatcctat atatgaaatc ccagaatcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cttgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagttttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgcttagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcgttatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt cattgaaaaa
     1021 atatc
//
LOCUS       DAVKJU010000131          801 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVKJU010000131 DAVKJU010000000
VERSION     DAVKJU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.131"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            343..567
                     /gene="pneA1"
                     /locus_tag="VW191_002015"
     CDS             343..567
                     /gene="pneA1"
                     /locus_tag="VW191_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HEU9624723.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            574..762
                     /gene="pneA2"
                     /locus_tag="VW191_002016"
     CDS             574..762
                     /gene="pneA2"
                     /locus_tag="VW191_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HEU9624724.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
BASE COUNT          288 a           99 c          159 g          255 t
ORIGIN      
        1 aaaaatactt gactaaataa aatatatctg ttagaataaa aacaaggaaa aagaaagggg
       61 tttcattgca tgagaaaaaa acgtggaatt aaaaaattag ttacatttgc attgctaggt
      121 gtttttatgt ttagtaatac aattccttac caacagttta ttcagaagaa taaactgttg
      181 gagattcgag tgcaatcgca aaagaagtcc aatggtcttg atgttgggaa ggcggattaa
      241 aacccgatat tttgcacgag aatgagagaa ttataattta tctgaaaaaa cgaaaaatat
      301 agagcaactt atttaacttt ttataaggag gagttattac ttatgaccaa ctttaattca
      361 aacgaaaaat tttgtggaaa aagtttaaaa tcattgtctg ctgatgaaat gagtttaatt
      421 tatggtgcat ctgatggtgc agagccacgt tggacgccta ctcctattat tttgaaatca
      481 gctgcagcta gctcgaaagt atgcattagt gcagctgttt ctggtattgg ggggctagtt
      541 agctacaata atgattgttt ggggtaaatt aaaatgaaaa atgattttgt aatcggaaag
      601 agtttgaaag aattatctct tgaagaaatg caattggttt atggtggcac agatggtgct
      661 gaccctagat caacaattat atgttctgct acattaagtt ttattgcatc atatttgggg
      721 tcagctcaga ctcgttgtgg caaagacaac aagaaaaaat aaaatattca attttaaaaa
      781 taaaagttgc tctttatttt c
//
LOCUS       DAVKJU010000132          794 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVKJU010000132 DAVKJU010000000
VERSION     DAVKJU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.132"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..612)
                     /locus_tag="VW191_002017"
     CDS             complement(<1..612)
                     /locus_tag="VW191_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEU9624725.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKK"
BASE COUNT          236 a          145 c          128 g          285 t
ORIGIN      
        1 ttttttatta gaattattta aagcgatgcg ttgagcctct ccctcttcct cgctaaaagt
       61 agctagagct ccgttttgaa taccattaca gctaacgaaa gctttagaaa attggagatt
      121 agagagattt tgtagggtca atgtaccaac aaaagcacct gtaatatcgc gataatttcc
      181 acctattaaa atcaaatctg ttaattttcg ttcgcttaaa atcagaaaaa caggtagact
      241 gttggttacg acgcggatat tgtcaatagg caactcacgc gcaaaaaact ctaatgttgt
      301 tcctggtcca atgaaaatag tttctctttc ttctactaga ctgcctgcaa aatgggctat
      361 ttcttgtttt tctgccgttt ggagggcttg tttttcaata tttgatcgct cattagtcaa
      421 aagggagttg gttcgaagtt tttcagctcc accatgcaca cgaatcagca aatctttatc
      481 agctaattcc tgtaaatagc gccttgcagt catatctgaa acggctattt cgtccataat
      541 ctgtttaact gttatggttc ctttactatt tactatctct aaaattttgg ttaatttttc
      601 ttgtttgagc atattatcac ctcgtttcct actactatct taccataaac tagctcatca
      661 ttcaaataca aaaacaacaa aataaaacaa aaacaaaaat atcgaggttt gttttcaaaa
      721 ctttcgatat ttttattaag ttattatttt gttgtttcta gtttactttt tgatggttaa
      781 gagtggtgga gaat
//
LOCUS       DAVKJU010000133          720 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVKJU010000133 DAVKJU010000000
VERSION     DAVKJU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.133"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..720
                     /locus_tag="VW191_002018"
                     /pseudo
     CDS             1..720
                     /locus_tag="VW191_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="catalyzes the formation of UDP-glucuronate from
                     UDP-glucose; frameshifted; incomplete; missing N-terminus
                     and C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
BASE COUNT          265 a           89 c          131 g          235 t
ORIGIN      
        1 cgcttccgac agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa
       61 cggtatcgaa gattctgagg gtaaaaccta atggaaagat tattttaaag tcaatagttc
      121 cgatcggctt ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc
      181 ctgaattttt gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg
      241 ttggagatga gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc
      301 atagtactgc caatattaaa aatgttatgt tagtttctcc tactgaagca gaagcaatta
      361 agcttttttc taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt
      421 ttgctgagag gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa
      481 gaattggaaa tttttataat aatccttctt ttggatttgg aggatattgt cttcccaaag
      541 atactaaaca attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg
      601 atatttcaaa cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa
      661 agtatagtag gaatatataa attggggatg aaatataatt cagataatta caaagagtca
//
LOCUS       DAVKJU010000134          568 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVKJU010000134 DAVKJU010000000
VERSION     DAVKJU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.134"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            1..568
                     /locus_tag="VW191_002019"
                     /pseudo
     CDS             1..568
                     /locus_tag="VW191_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          189 a          108 c          105 g          166 t
ORIGIN      
        1 aatccagatg cttatttgac tgaaatagct tctgaatttg gctgtcatcc aactaccatc
       61 cactatgcgc tcaaagctat gggctacact cgaaaaaaag aaccacacct actatgaaca
      121 agacccagaa aaagtagcca tatttcttaa aaattttaat agtttaaagc acctagcacc
      181 tgtttagatt gacgaaacag gattcgatac ttatttttat cgagaatatg gtcgctcatt
      241 aaaaggtcag ttaataagag gtaaagtatc tggaagaaga tatcagagga tttctttggt
      301 tgcaggtcga acaaatagtg agttaatcgc tccaatgact tacgaagaga tgatgacgag
      361 cgactttttt gaagcttggt ttcagaagtt tttcttacca acattaacca caccatcggt
      421 tattattatg gataatgtaa gattccatag aatggggaag ctagaacttt tatgcgaaga
      481 gtttgggcat aaacttttac ctcttcctcc ctactcgcct gagtacaatc ttattgagaa
      541 aacatgggct catatcaaaa agcacctc
//
LOCUS       DAVKJU010000135          566 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVKJU010000135 DAVKJU010000000
VERSION     DAVKJU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.135"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(<1..>566)
                     /locus_tag="VW191_002020"
     CDS             complement(<1..>566)
                     /locus_tag="VW191_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU9624726.1"
                     /translation="YCSQILQLLLKYFDKNPNKISEVYSILEANYGLVTEREYIDYTL
                     AENTISELANLDLTKSYNQELIVTILKQFLKIEIERTETHEEKFTFGRYKVPDSEKLK
                     QYHRSILKLLANLYNIGSCETRFYIEKMLYDYRRKILTYSESHRNTIFGDLRNIRKLF
                     FNDIKNLSMIGEKIVYALHKAEVKENLP"
BASE COUNT          186 a           95 c           63 g          222 t
ORIGIN      
        1 tcggtaaatt ttctttcact tcggctttat gcaaagcata aactattttt tctccaatca
       61 tagataagtt tttaatgtca ttaaaaaata atttcctaat atttctcaaa tctccaaaga
      121 tagtattacg atgactttct gaataggtca aaatttttct tctgtaatca tatagcattt
      181 tctcaatata aaatctagtt tcacaactcc ctatattata caaattcgct aaaagtttta
      241 gtatacttct atgatactgt tttaattttt cactatccgg gactttatag cgcccaaaag
      301 taaatttctc ttcatgagtc tctgttcttt cgatttcaat tttaagaaac tgcttcaaaa
      361 ttgtcacgat taattcttgg ttataacttt tcgtcaaatc aagattagca agttcagaaa
      421 tggtattttc agctaaggta taatcaatat attctctttc tgtaaccaac ccgtagtttg
      481 cttcaagaat actataaacc tcagaaattt tattaggatt tttatcaaag tactttaaca
      541 ataattggag aatttgacta caatat
//
LOCUS       DAVKJU010000136          535 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVKJU010000136 DAVKJU010000000
VERSION     DAVKJU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.136"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>535
                     /locus_tag="VW191_002021"
     CDS             <1..>535
                     /locus_tag="VW191_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU9624727.1"
                     /translation="PYLKEGGEYQIKNLSYKFTDDECLSLKDISFKLGKIYGIIGSNG
                     RGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELR
                     LGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFIFFDEPS
                     SGMDYSNMIKISELINKY"
BASE COUNT          209 a           65 c          103 g          158 t
ORIGIN      
        1 ccttatttaa aagaaggtgg agagtatcag ataaaaaatc ttagctacaa atttactgat
       61 gatgagtgtt taagcttaaa agatatttca ttcaagcttg ggaaaattta tggcataata
      121 ggatccaacg gacgaggaaa atcaacgctt ttaagatgtt taataggtct tgagaaaaaa
      181 tcaaaagaag aaatttattt taagggagag aagctatcta aaaaagaaag actcaaaaac
      241 tcttcacttg ttatgcaaga tgtaaatcat caattattca cagatgaagt attcaacgag
      301 cttagattag gagtaaagaa ttttgatgaa gaaaaggcga aaatcatttt aaaagattta
      361 ggcctggacg aatttattga aaggcatccg atgagtttat caggagggca aaagcaaagg
      421 cttgcaatag catctgttat gtgcaagaat tctccattta tcttttttga cgaaccttca
      481 agtggtatgg attattccaa tatgataaaa atatctgaac tgattaataa gtata
//
LOCUS       DAVKJU010000137          434 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVKJU010000137 DAVKJU010000000
VERSION     DAVKJU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.137"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>434
                     /locus_tag="VW191_002022"
     CDS             <1..>434
                     /locus_tag="VW191_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HEU9624728.1"
                     /translation="DAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRA
                     NGTQTYKATVKVYGNKDGKADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDV
                     PAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLLKASD"
BASE COUNT          151 a           93 c           90 g          100 t
ORIGIN      
        1 gacgctaaaa acccagccct atctccacta ggtgaaaacg tgaagaccaa aggtcaatac
       61 ttctatcaag tagccttgga cggaaatgta gctggcaaag aaaaacaagc gctcattgac
      121 cagttccgag caaatggtac tcaaacttat aaagctactg ttaaagttta cggaaataaa
      181 gacggtaaag ctgacttgac taatctagtt gctactaaaa atgtagacat caacatcaat
      241 ggattagttg ctaaagaaac agttcaaaaa gccgttgcag acaacgttaa agacagtatc
      301 gatgttccag cagcctactt agaaaaagcc aagggtgaag gtccattcac agcaggtgtc
      361 aaccatgtga ttccatacga actcttcgca ggtgatggca tgttgactcg tctcttgctc
      421 aaggcatctg acaa
//
LOCUS       DAVKJU010000138          402 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVKJU010000138 DAVKJU010000000
VERSION     DAVKJU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.138"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>402
                     /locus_tag="VW191_002023"
     CDS             <1..>402
                     /locus_tag="VW191_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEU9624729.1"
                     /translation="FKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTD
                     RKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYA
                     HPYASWERGTNENHNRLIRRWLPKGTKKMTP"
BASE COUNT          126 a           85 c           86 g          105 t
ORIGIN      
        1 ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa gctatcaatc ttcgcttgga
       61 gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact actgcttgct
      121 tgtcttgacg gatcgaaaga gtagacatca gatcatccga ttgattccaa ataaaagtgc
      181 tgaggtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc tttccatcac
      241 ggcagataat ggaacggaat tcaatcgctt gtttgatgta ttttctgagg agcacatcta
      301 ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca acaggctcat
      361 tcgtagatgg ttacctaagg gaaccaagaa aatgactccc aa
//
LOCUS       DAVKJU010000139          350 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVKJU010000139 DAVKJU010000000
VERSION     DAVKJU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.139"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            <1..>350
                     /locus_tag="VW191_002024"
     CDS             <1..>350
                     /locus_tag="VW191_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEU9624730.1"
                     /translation="DVPYDIPDTWEWVRIKSIYWNFGQNKPEKSFRYIDTSSIDRKKN
                     IINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIV
                     LDTLLNETYLKYYL"
BASE COUNT          125 a           48 c           59 g          118 t
ORIGIN      
        1 ttgatgttcc ttatgatatt cctgatactt gggagtgggt gaggataaaa tcaatttatt
       61 ggaattttgg gcaaaataag ccagagaaat cctttaggta tatagatacg tctagtattg
      121 atagaaaaaa gaacataatc aactacaaaa atctacaata tctttcacct gaacaagcgc
      181 cttcccgtgc tagaaaatta gtttcgcaga atagtgtctt attttcaaca gttagaccat
      241 atctaaaaaa tattgctgta gttagagaac ttaaagagta tttgatagct agtacagcat
      301 ttattgtttt ggatacttta cttaacgaaa catatttgaa atattattta
//
LOCUS       DAVKJU010000140          344 bp    DNA     linear   BCT 18-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00074823
            SAMN33606210-rid17942883.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVKJU010000140 DAVKJU010000000
VERSION     DAVKJU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33606210
            Sequence Read Archive: SRR23727419
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001722461.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001722461.1
            Genome Coverage       :: 283x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 00:26:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,025
            CDSs (total)                      :: 1,978
            Genes (coding)                    :: 1,875
            CDSs (with protein)               :: 1,875
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 103
            CDSs (without protein)            :: 103
            Pseudo Genes (ambiguous residues) :: 0 of 103
            Pseudo Genes (frameshifted)       :: 56 of 103
            Pseudo Genes (incomplete)         :: 44 of 103
            Pseudo Genes (internal stop)      :: 35 of 103
            Pseudo Genes (multiple problems)  :: 26 of 103
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33606210-rid17942883.denovo.140"
                     /strain="AUSMDU00074823"
                     /serotype="35B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST558"
     gene            complement(90..>344)
                     /locus_tag="VW191_002025"
     CDS             complement(90..>344)
                     /locus_tag="VW191_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEU9624731.1"
                     /translation="ANGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGA
                     MKASQWFKVSDKWYYVNGLGALAVNTTVDGYKVNANGEWV"
BASE COUNT          100 a           81 c           53 g          110 t
ORIGIN      
        1 aatacgaaca taaatctatt caatcgaacc ttatctttgt ttcaaaatta aaatgtcaaa
       61 tgttcttaac atgatttaat ttaatcggct taaacccatt caccattggc attgacttta
      121 tagccatcta cagttgtgtt gactgcaagg gcacctaaac cattgacata gtaccattta
      181 tctgatactt tgaaccattg gcttgctttc atagcacctg atgcttcaag atagtaccag
      241 gtatctccat ctttcaccca acctgttgcc attgaaccgt tagcgttgag gtagtaccat
      301 gaaccgttga ctttagccca acctgtagcc atagcaccgt tagc
//
